-- dump date 20120504_133510 -- class Genbank::CDS -- table cds -- table main -- field 1 id -- field 2 GI -- field 3 GeneID -- field 4 chrom_position -- field 5 chromosome -- field 6 codon_start -- field 7 contig -- field 8 description -- field 9 end_pos -- field 10 gene -- field 11 gene_id -- field 12 name -- field 13 organism -- field 14 product -- field 15 protein_id -- field 16 start_pos -- field 17 strand -- field 18 taxid -- field 19 type -- header -- id GI GeneID chrom_position chromosome codon_start contig description end_pos gene gene_id name organism product protein_id start_pos strand taxid type YP_931506.1 119896293 4610202 1..1443 1 NC_008702.1 binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.; chromosomal replication initiation protein 1443 dnaA 4610202 dnaA Azoarcus sp. BH72 chromosomal replication initiation protein YP_931506.1 1 D 62928 CDS YP_931507.1 119896294 4608630 1502..2611 1 NC_008702.1 DNA polymerase III beta chain (EC 2.7.7.7). DNA polymerase III is a complex multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3 to 5 exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA It slides freely (bidirectional and ATP- independent) along duplex DNA (By similarity). InterPro: DNA polymerase III beta chain dnan: DNA polymerase III beta subunit; High confidence in function and specificity; DNA-directed DNA polymerase 2611 dnaN 4608630 dnaN Azoarcus sp. BH72 DNA-directed DNA polymerase YP_931507.1 1502 D 62928 CDS YP_931508.1 119896295 4608638 2821..5310 1 NC_008702.1 negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit B 5310 gyrB 4608638 gyrB Azoarcus sp. BH72 DNA gyrase subunit B YP_931508.1 2821 D 62928 CDS YP_931509.1 119896296 4608976 5505..7346 1 NC_008702.1 METHYLATION OF SPECIFIC ADENINE RESIDUES; REQUIRED FOR BOTH RESTRICTION AND MODIFICATION ACTIVITIES (BY SIMILARITY), TREMBL:Q7VCN2 (55% identity). InterPro (IPR002296): N6 adenine-specific DNA methyltransferase,N12 class. InterPro (IPR003356): N-6 DNA methylase. InterPro (IPR003665): Type I restriction-modification system M subunit. Pfam (PF02384): N-6 DNA Methylase. Pfam (PF02506): Type I restriction modification system, M protein.; High confidence in function and specificity; site-specific DNA-methyltransferase 7346 hsdM 4608976 hsdM Azoarcus sp. BH72 site-specific DNA-methyltransferase YP_931509.1 5505 D 62928 CDS YP_931510.1 119896297 4609031 7453..8910 1 NC_008702.1 Putative restriction modification gene,; Function unclear; putative restriction modification gene 8910 mloB 4609031 mloB Azoarcus sp. BH72 putative restriction modification gene YP_931510.1 7453 D 62928 CDS YP_931511.1 119896298 4609226 8919..9038 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to the data bank no domains predicted no signal peptide no TMHS; hypothetical protein 9038 4609226 azo0006 Azoarcus sp. BH72 hypothetical protein YP_931511.1 8919 D 62928 CDS YP_931512.1 119896299 4606149 9035..10384 1 NC_008702.1 S subunit, DNA SPECIFICITY SUBUNIT REQUIRED FOR RESTRICTION AND MODIFICATION, TREMBL:Q9AAH7 (31% identity); TREMBL:Q8PIJ8 (28% identity). Pfam (PF01420): Type I restriction modification DNA specificity domain. InterPro (IPR000055): Restriction modification system DNA specificity domain.; High confidence in function and specificity; Type I site-specific deoxyribonuclease 10384 hsdS 4606149 hsdS Azoarcus sp. BH72 Type I site-specific deoxyribonuclease YP_931512.1 9035 D 62928 CDS YP_931513.1 119896300 4609033 10381..11256 1 NC_008702.1 Conserved hypothetical protein. Homology to RB11641 of R.baltica of 42% (tremble:Q7TTG8). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 11256 4609033 azo0008 Azoarcus sp. BH72 hypothetical protein YP_931513.1 10381 D 62928 CDS YP_931514.1 119896301 4606150 11468..11794 1 NC_008702.1 Transcriptional regulatory protein, 35% identity to TrEMBL;Q89JK0. Has Prosite, PS01117; HTH_MARR_1 domain; The marR-type HTH domain is a DNA-binding, winged helix-turn-helix (wHTH)domain of about 135 amino acids present in transcription regulators of the marR/slyA family,involved in the development of antibiotic resistance; Function unclear; transcriptional regulator 11794 4606150 azo0009 Azoarcus sp. BH72 transcriptional regulator YP_931514.1 11468 D 62928 CDS YP_931515.1 119896302 4606151 11948..15061 1 NC_008702.1 SUBUNIT R IS REQUIRED FOR BOTH NUCLEASE AND ATPASE ACTIVITIES BUT NOT FOR MODIFICATION (BY SIMILARITY),TREMBL:Q9AAH5 (64% identity); TREMBL:Q8FUH0 (40% identity). InterPro (IPR001410): DEAD/DEAH box helicase TIGRFAM (TIGR00348): Type I site-specific deoxyribonuclease, HsdR family.; High confidence in function and specificity; Type I site-specific deoxyribonuclease 15061 hsdR 4606151 hsdR Azoarcus sp. BH72 Type I site-specific deoxyribonuclease YP_931515.1 11948 D 62928 CDS YP_931516.1 119896303 4609032 complement(15231..16085) 1 NC_008702.1 The short-chain dehydrogenases/reductases family is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases, similarity to SWISSPROT:P37959 (35% identity); TREMBL:Q930L8 (44% identity); TREMBL:Q9I3W5 (41% identity). Pfam (PF00106): Short chain dehydrogenase. InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase.; Specificity unclear; short-chain dehydrogenase 16085 4609032 azo0011 Azoarcus sp. BH72 short-chain dehydrogenase YP_931516.1 15231 R 62928 CDS YP_931517.1 119896304 4606152 16203..17150 1 NC_008702.1 Transcriptional regulator, LysR family,; High confidence in function and specificity; LysR family transcriptional regulator 17150 bdhR 4606152 bdhR Azoarcus sp. BH72 LysR family transcriptional regulator YP_931517.1 16203 D 62928 CDS YP_931518.1 119896305 4608384 17232..17876 1 NC_008702.1 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs .; Family membership; pseudouridine synthase A 17876 rluA1 4608384 rluA1 Azoarcus sp. BH72 pseudouridine synthase A YP_931518.1 17232 D 62928 CDS YP_931519.1 119896306 4609731 complement(18010..18567) 1 NC_008702.1 An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group; flavodoxin FldA 18567 nifF1 4609731 nifF1 Azoarcus sp. BH72 flavodoxin FldA YP_931519.1 18010 R 62928 CDS YP_931520.1 119896307 4609361 complement(18971..19501) 1 NC_008702.1 Conserved hypothetical protein, 50% identitcal(67% similarity) to TrEMBL;Q888U7.TrEMBL;Q747X0(33% identity). Has PF03473, MOSC domain;IPR005302; The MOSC (MOCO sulfurase C-terminal) domain is a superfamily of beta-strand-rich domains identified in the molybdenum cofactor sulfurase and several other proteins from both prokaryotes and eukaryotes. These MOSC domains contain an absolutely conserved cysteine and occur either as stand-alone forms such as P32157, or fused to other domains such as NifS-like catalytic domain in Molybdenum cofactor sulfurase. The MOSC domain is predicted to be a sulfur-carrier domain that receives sulfur abstracted by the pyridoxal phosphate-dependent NifS-like enzymes, on its conserved cysteine,and delivers it for the formation of diverse sulfur-metal clusters.; Function unclear; hypothetical protein 19501 4609361 azo0015 Azoarcus sp. BH72 hypothetical protein YP_931520.1 18971 R 62928 CDS YP_931521.1 119896308 4606153 complement(19505..19855) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03002995 of Dechloromonas aromatica of 34% (ZP_00348572.1). No signal peptide present. TMHMM reporting one transmembrane helix. No domains predicted.; Conserved hypothetical protein; hypothetical protein 19855 4606153 azo0016 Azoarcus sp. BH72 hypothetical protein YP_931521.1 19505 R 62928 CDS YP_931522.1 119896309 4606154 complement(19882..20355) 1 NC_008702.1 Hypothetical protein, 28% identity to TrEMBL;Q8E8U4. Has PF07049, Protein of unknown function (DUF1332); IPR010755;This family consists of several hypothetical bacterial proteins of around 165 residues in length. The function of this family is unknown.; Function unclear; hypothetical protein 20355 4606154 azo0017 Azoarcus sp. BH72 hypothetical protein YP_931522.1 19882 R 62928 CDS YP_931523.1 119896310 4606155 complement(20480..21061) 1 NC_008702.1 InterPro (IPR002818): ThiJ/PfpI. Pfam (PF01965): DJ-1/PfpI family.; Specificity unclear; ThiJ/PfpI family protein 21061 4606155 azo0018 Azoarcus sp. BH72 ThiJ/PfpI family protein YP_931523.1 20480 R 62928 CDS YP_931524.1 119896311 4606156 21263..22402 1 NC_008702.1 This family includes extracellular ligand binding domains of a wide range of receptors and it also includes the bacterial amino acid binding proteins of known structure. Similar to trembl|Q8XVG7 (38%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Function unclear; extracellular ligand binding protein 22402 4606156 azo0019 Azoarcus sp. BH72 extracellular ligand binding protein YP_931524.1 21263 D 62928 CDS YP_931525.1 119896312 4606157 complement(22404..23309) 1 NC_008702.1 Probable transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 23309 4606157 azo0020 Azoarcus sp. BH72 LysR family transcriptional regulator YP_931525.1 22404 R 62928 CDS YP_931526.1 119896313 4606158 23470..23928 1 NC_008702.1 Probable isoquinoline 1-oxidoreductase, alpha subunit Homology to iroA of B. diminuta of 58% (sprot|IORA_BREDI). InterPro: [2Fe-2S] binding domain (IPR002888); Ferredoxin (IPR001041) Pfam: 2FE-2S iron-sulfur cluster binding domein, [2Fe-2S] binding domain no signal peptide no TMHs; Specificity unclear; isoquinoline 1-oxidoreductase subunit alpha 23928 iorA 4606158 iorA Azoarcus sp. BH72 isoquinoline 1-oxidoreductase subunit alpha YP_931526.1 23470 D 62928 CDS YP_931527.1 119896314 4609085 23939..26152 1 NC_008702.1 Putative isoquinoline 1-oxidoreductase, beta subunit Homology to iorB of B. diminuta of 34% (sprot|IORB_BREDI). InterPro: Aldehyde oxidase and xanthine dehydrogenase C terminus (IPR000674) Pfam: Aldehyde oxidase and xanthine dehydrogenase signal peptide no TMHs; Family membership; putative isoquinoline 1-oxidoreductase subunit beta 26152 iorB 4609085 iorB Azoarcus sp. BH72 putative isoquinoline 1-oxidoreductase subunit beta YP_931527.1 23939 D 62928 CDS YP_931528.1 119896315 4609086 26175..27197 1 NC_008702.1 Conserved hypothetical protein (Xanthine like-dehydrogenase accessory factor). Homology to Rsc1464 (hyp. protein) of R. solanacearum of 66% (CAD15165). InterPro: DUF182 Pfam: uncharacterized BCR, COG1975 no signal peptide no TMHs; hypothetical protein 27197 4609086 azo0023 Azoarcus sp. BH72 hypothetical protein YP_931528.1 26175 D 62928 CDS YP_931529.1 119896316 4606159 27194..27763 1 NC_008702.1 Purine catabolism protein pucB. TREMBL:Q8XZ0: 40% identity, 47% similarity Required for xanthine dehydrogenase activity. Could be involved in formation of the molybdenum cofactor required by xanthine dehydrogenase. FUNCTION: Links a guanosine 5'-phosphate to molydopterin (MPT) forming molybdopterin guanine dinucleotide (MGD) (By similarity). PATHWAY: Molybdenum cofactor biosynthesis. SUBCELLULAR LOCATION: Cytoplasmic (By similarity). SIMILARITY: Belongs to the mobA family. Pfam:UPF0007:Uncharacterized protein family UPF000 ispD: 4-diphosphocytidyl-2C-methyl-D-er Non-secretory protein with signal peptide probability: 0 No TMH's (TMHMM predicted).; Function unclear; hypothetical protein 27763 4606159 azo0024 Azoarcus sp. BH72 hypothetical protein YP_931529.1 27194 D 62928 CDS YP_931530.1 119896317 4606160 28052..28618 1 NC_008702.1 Hypothetical protein yddH. TREMBL:Q7NTI1:66% identity, 76% similarity InterPro:IPR002563; Flavin_Reduct. Pfam:PF01613; Flavin_Reduct; InterPro: Flavin reductase-like domain Non-secretory protein with no signal peptide. No transmembrane helices TIGR00357: PilB-related protein; High confidence in function and specificity; hypothetical protein 28618 4606160 azo0025 Azoarcus sp. BH72 hypothetical protein YP_931530.1 28052 D 62928 CDS YP_931531.1 119896318 4606161 complement(28631..29569) 1 NC_008702.1 dTDP-glucose 4,6-dehydratase, AcbB,probably involved in the biosynthesis of the acarviose moiety of the alpha-glucosidase inhibitor acarbose. Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily. 30% dTDP_gluc_dehyt.IPR001509; Epimerase_Dh. Pfam:PF01370; Epimerase; 1. TIGRFAMs:TIGR01181; dTDP_gluc_dehyt; 1.; High confidence in function and specificity; putative dTDP-glucose 4-6-dehydratase 29569 acbB 4606161 acbB Azoarcus sp. BH72 putative dTDP-glucose 4-6-dehydratase YP_931531.1 28631 R 62928 CDS YP_931532.1 119896319 4608253 29590..30477 1 NC_008702.1 Putative glycosyl transferase. Weak Homology with hits spanning the entire length of protein. 24% identitcal to TrEMBL;Q8F209.TrEMBL; Q89D69(33% identity) Has PF00535,Glycosyl transferase:IPR001173;Diverse family,transferring sugar from UDP-glucose, UDP-N-acetyl-galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids. The biosynthesis of disaccharides,oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These are enzymes that catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules,forming glycosidic bonds.; Family membership; glycosyltransferase 30477 exoU 4608253 exoU Azoarcus sp. BH72 glycosyltransferase YP_931532.1 29590 D 62928 CDS YP_931533.1 119896320 4608706 complement(30502..30732) 1 NC_008702.1 Conserved hypothetical membrane protein Homology to cv0580 of C. violaceum of 41% (trembl|Q7P0I7(SRS)) no domains predicted no signal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 30732 4608706 azo0028 Azoarcus sp. BH72 hypothetical protein YP_931533.1 30502 R 62928 CDS YP_931534.1 119896321 4606162 30906..31676 1 NC_008702.1 Similar to a tellurium resistance protein TerC. Belongs to the terC family. This family contains a number of integral membrane proteins that also contains the TerC protein. TerC has been implicated in resistance to tellurium. Similar to a putative membrane protein TerC,80% identity to TrEMBL; Q5P410 from Azoarcus sp EnN1. 50% RNA_rec_mot.IPR005496; TerC. Pfam:PF03741; TerC; 1. TIGR:SPTO2691. TMHelix:5.; Conserved hypothetical protein; hypothetical protein 31676 4606162 azo0029 Azoarcus sp. BH72 hypothetical protein YP_931534.1 30906 D 62928 CDS YP_931535.1 119896322 4606163 complement(31735..32625) 1 NC_008702.1 HTH-type transcriptional regulator gltR. POSITIVE REGULATOR OF GLUTAMATE BIOSYNTHESIS (GLTAB GENES). NEGATIVELY REGULATES ITS OWN EXPRESSION. Similar to SWISSPROT: sprot|GLTR_BACSU (33% Bacillus subtilis, HTH-type transcriptional regulator GltR) InterPro: IPR000847 HTH_LysR. IPR009058 Winged helix DNA-binding. Pfam: PF00126 Bacterial regulatory helix-turn-helix protein,lysR family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; LysR family transcriptional regulator 32625 gltR 4606163 gltR Azoarcus sp. BH72 LysR family transcriptional regulator YP_931535.1 31735 R 62928 CDS YP_931536.1 119896323 4608917 32784..34019 1 NC_008702.1 Permease member of the Major Facilitator Superfamily (MFS).MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. 25% MFS.IPR005828; Sub_transporter. Pfam:PF00083; sugar_tr; 1. TMHelix: 10.; High confidence in function and specificity; putative MFS transporter permease 34019 4608917 azo0031 Azoarcus sp. BH72 putative MFS transporter permease YP_931536.1 32784 D 62928 CDS YP_931537.1 119896324 4606164 complement(34326..34814) 1 NC_008702.1 Conserved hypothetical protein. Homology to nfa21510 of Nocardia farcinica of 31% (trembl:Q5YXU4). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 34814 4606164 azo0032 Azoarcus sp. BH72 hypothetical protein YP_931537.1 34326 R 62928 CDS YP_931538.1 119896325 4606165 35113..35601 1 NC_008702.1 Conserved hypothetical protein. Homology to nfa21510 of Nocardia farcinica of 42% (trembl:Q5YXU4). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 35601 4606165 azo0033 Azoarcus sp. BH72 hypothetical protein YP_931538.1 35113 D 62928 CDS YP_931539.1 119896326 4606166 35640..37502 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2042 of P. putida of 34% (TrEMBL;Q88L93) Has PF06980:(IPR010727)Protein of unknown function (DUF1302);This family contains a number of hypothetical bacterial proteins of unknown function that are approximately 600 residues long. Most family members seem to be from Pseudomonas. No TMHs signal peptide present.; Conserved hypothetical protein; hypothetical protein 37502 4606166 azo0034 Azoarcus sp. BH72 hypothetical protein YP_931539.1 35640 D 62928 CDS YP_931540.1 119896327 4606167 37514..38869 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA3421 of P. aeruginosa of 50% (TrEMBL;Q9HYI4) Has (IPR010752)PF07044:Protein of unknown function (DUF1329);This family consists of several hypothetical bacterial proteins of around 475 residues in length. The majority of family members are from Pseudomonas species but the family also contains sequences from Shewanella oneidensis and Thauera aromatica. No TMHs signal peptide present; Conserved hypothetical protein; hypothetical protein 38869 4606167 azo0035 Azoarcus sp. BH72 hypothetical protein YP_931540.1 37514 D 62928 CDS YP_931541.1 119896328 4606168 38945..40102 1 NC_008702.1 Conserved hypothetical BNR domain protein. Homology to pp3201 of P. putida of 40% (trembl|Q88I01). InterPro: BNR repeat (IPR002860) Pfam: BNR repeat BNR repeats are short repeats never found closer than 40 residues together, which suggests that the repeat is structurally longer. These repeats are found in many glycosyl hydrolases as well as other extracellular proteins of unknown function. singal peptide no TMHs; Function unclear; hypothetical protein 40102 4606168 azo0036 Azoarcus sp. BH72 hypothetical protein YP_931541.1 38945 D 62928 CDS YP_931542.1 119896329 4606169 40095..42521 1 NC_008702.1 Putative membrane protein MJ1562. TREMBL:Q88LB6: 47% identity, 63% similarity InterPro:IPR000731; SSD_5TM InterPro: HMGCR/Patched 5TM box Pfam:Patched:Patched family 2A067: efflux transporter putative Signal peptide absent. Presence of 12 transmembrane helices; Family membership; RND efflux transporter 42521 4606169 azo0037 Azoarcus sp. BH72 RND efflux transporter YP_931542.1 40095 D 62928 CDS YP_931543.1 119896330 4606170 42527..43336 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases. EntA,a 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase enzyme,is involved in the third stage of enterobactin biosynthesis and converts isochorismate to 2,3-dihydroxybenzoic acid (DHBA), SWISSPROT:Q10855 (37% identity); TREMBL:Q89M15 (36% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. InterPro (IPR003560): 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 43336 4606170 azo0038 Azoarcus sp. BH72 short-chain dehydrogenase YP_931543.1 42527 D 62928 CDS YP_931544.1 119896331 4606171 43466..45670 1 NC_008702.1 Conservd hypothetical protein, 79% identity(85% similarity) to TrEMBL;Q88GU7. Has PF02566, OsmC-like protein; IPR003718; Osmotically inducible protein C (OsmC) (P23929) is a stress -induced protein found in E. Coli. This family also contains a organic hydroperoxide detoxification protein (O68390) that has a novel pattern of oxidative stress regulation. Has PF02624, YcaO-like fatty acid binding protein;IPR003776, DUF181;This is a family of proteins of unknown function.; hypothetical protein 45670 4606171 azo0039 Azoarcus sp. BH72 hypothetical protein YP_931544.1 43466 D 62928 CDS YP_931545.1 119896332 4606172 complement(45701..46612) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 46612 4606172 azo0040 Azoarcus sp. BH72 AraC family transcriptional regulator YP_931545.1 45701 R 62928 CDS YP_931546.1 119896333 4606173 46800..47561 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q9I376 (49% identity); SWISSPROT:P50197 (44% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 47561 4606173 azo0041 Azoarcus sp. BH72 short-chain dehydrogenase YP_931546.1 46800 D 62928 CDS YP_931547.1 119896334 4606174 47901..48494 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bll2189 of B. japonicum of 48% (trembl|Q89T61). Has PF06577, Protein of unknown function (DUF1134);IPR008325,UCP033924; This family consists of several hypothetical bacterial proteins of unknown function. Signal peptide. TMH present in signal peptide.; Conserved hypothetical protein; hypothetical protein 48494 4606174 azo0042 Azoarcus sp. BH72 hypothetical protein YP_931547.1 47901 D 62928 CDS YP_931548.1 119896335 4606175 complement(48804..49574) 1 NC_008702.1 Hypothetical UPF0271 protein PYRAB09930. This family includes LamB. The lam locus of Aspergillus nidulans consists of two divergently transcribed genes,lamA and lamB, involved in the utilization of lactams such as 2-pyrrolidinone. Both genes are under the control of the positive regulatory gene amdR and are subject to carbon and nitrogen metabolite repression [1]. The exact molecular function of the proteins in this family is unknown. SPROT:Q89LH6: 47% identity, 62% similarity InterPro:IPR005501; LamB_YcsF. Pfam:PF03746; LamB_YcsF urease_gam: urease gamma subunit Nonsecretory protein with Signal peptide probability 0 No transmembrane helices; Family membership; hypothetical protein 49574 4606175 azo0043 Azoarcus sp. BH72 hypothetical protein YP_931548.1 48804 R 62928 CDS YP_931549.1 119896336 4606176 complement(49571..50671) 1 NC_008702.1 Conserved hypothetical urea amidolyase-related protein. Homology to ybgK of E. coli of 36% (sprot|YBGK_ECOLI). InterPro: DUF183 (IPR003778) Uncharacterized domain in proteins of unknown function. This domain is found in a multifunctional enzyme, urea amidolyase, from yeast. Pfam: DFU182, uncharcterized ACR,COG1984 no signal peptide no TMHs; Function unclear; hypothetical protein 50671 4606176 azo0044 Azoarcus sp. BH72 hypothetical protein YP_931549.1 49571 R 62928 CDS YP_931550.1 119896337 4606177 complement(50668..51462) 1 NC_008702.1 Conserved hypothetical protein ybgJ. Homology to ybgJ of E. coli of 41% (sprot|YBGJ_ECOLI) InterPro: DUF213 (IPR003833) Pfam: DUF213, uncharacterized ACR, COG2049 no signal peptide no TMHs; Function unclear; hypothetical protein 51462 4606177 azo0045 Azoarcus sp. BH72 hypothetical protein YP_931550.1 50668 R 62928 CDS YP_931551.1 119896338 4606178 complement(51590..52516) 1 NC_008702.1 Hypothetical haloacetate dehalogenase H-1 (EC 3.8.1.3). Haloacetate + H(2)O = glycolate + halide TREMBL:Q8Y2S9: 58% identity, 67% similarity Gene name:dehH from Ralstonia solanacearum genome project. InterPro: Alpha/beta hydrolase fold InterPro:IPR000073; A/b_hydrolase. IPR000379: Ser_estrs. Pfam:PF00561; Abhydrolase_1 nadp_idh_euk: isocitrate dehydrogenase No TMH present absence of signal peptide.; Family membership; haloacetate dehalogenase 52516 dehH 4606178 dehH Azoarcus sp. BH72 haloacetate dehalogenase YP_931551.1 51590 R 62928 CDS YP_931552.1 119896339 4608618 complement(52582..53337) 1 NC_008702.1 Conserved hypothetical secreted protein Homology to pa4487 of P. aeruginosa of 52% (trembl|Q9HVT4(SRS)) no domains predicted signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 53337 4608618 azo0047 Azoarcus sp. BH72 hypothetical protein YP_931552.1 52582 R 62928 CDS YP_931553.1 119896340 4606179 complement(53315..54151) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc3028 of R. solanacearum of 57% (trembl|Q8XV04(SRS)) no domains predicted signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 54151 4606179 azo0048 Azoarcus sp. BH72 hypothetical protein YP_931553.1 53315 R 62928 CDS YP_931554.1 119896341 4606180 complement(54285..56000) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA4488 of P. aeruginosa of 47% (trembl|Q9HVT3(SRS)) No domains predicted. No TMHs. Singal Peptide Present.; Conserved hypothetical protein; hypothetical protein 56000 4606180 azo0049 Azoarcus sp. BH72 hypothetical protein YP_931554.1 54285 R 62928 CDS YP_931555.1 119896342 4606181 complement(56050..57162) 1 NC_008702.1 This protein degrades water-insoluble and water-soluble PHB to monomeric D(-)-3-hydroxybutyrate,TREMBL:Q9LBN6 (35% identity); TREMBL:Q46334 (34% identity). SignalP predicting signal peptide.; High confidence in function and specificity; Poly (3-hydroxybutyrate) depolymerase 57162 phaZ 4606181 phaZ Azoarcus sp. BH72 Poly (3-hydroxybutyrate) depolymerase YP_931555.1 56050 R 62928 CDS YP_931556.1 119896343 4609538 complement(57268..58020) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA624 of Azoarcus sp. EbN1 of 52% (gnl|keqq|eba:ebA624(KEGG)) . no domains predicted. no signal peptide. 3 TMHs; hypothetical protein 58020 4609538 azo0051 Azoarcus sp. BH72 hypothetical protein YP_931556.1 57268 R 62928 CDS YP_931557.1 119896344 4606182 58066..59952 1 NC_008702.1 Hypothetical secreted protein. No homology of the entire protein to the data bank. InterPro:Esterase/lipase/thioesterase family active site Pfam:Putative serine esterase (DUF676) This family of proteins are probably serine esterase type enzymes. Signal peptide present (Signal P predicted) No transmembrane helices.; hypothetical protein 59952 4606182 azo0052 Azoarcus sp. BH72 hypothetical protein YP_931557.1 58066 D 62928 CDS YP_931558.1 119896345 4606183 complement(59974..61287) 1 NC_008702.1 Probable two component sensor histidine kinase,; Specificity unclear; sensor histidine kinase 61287 rstB 4606183 rstB Azoarcus sp. BH72 sensor histidine kinase YP_931558.1 59974 R 62928 CDS YP_931559.1 119896346 4609829 complement(61284..62054) 1 NC_008702.1 Two component response regulator,; Specificity unclear; two-component response regulator 62054 rstA 4609829 rstA Azoarcus sp. BH72 two-component response regulator YP_931559.1 61284 R 62928 CDS YP_931560.1 119896347 4609828 62241..62975 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:O54136 (41% identity); TREMBL:Q8RCZ7 (40% identity). Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; ABC transporter ATP-binding protein 62975 4609828 azo0055 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931560.1 62241 D 62928 CDS YP_931561.1 119896348 4606184 62972..64369 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv3942 of C. violaceum of 30% (trembl|Q7NR42) InterPro: DUF214 (IPR003838) Pfam: Predicted permease This is a family of predicted permeases and hypothetical transmembrane proteins. One member has been shown to transport lipids targeted to the outer membrane across the inner membrane, a step which requires ATP. Signal peptide 3 TMHs; Conserved hypothetical protein; hypothetical protein 64369 4606184 azo0056 Azoarcus sp. BH72 hypothetical protein YP_931561.1 62972 D 62928 CDS YP_931562.1 119896349 4606185 64362..65171 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BPSS2323 of Burkholderia pseudomallei of 45% (trembl:Q63HU7). no domains predicted. singal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 65171 4606185 azo0057 Azoarcus sp. BH72 hypothetical protein YP_931562.1 64362 D 62928 CDS YP_931563.1 119896350 4606186 65168..66349 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. signal peptide TMHs inside of the signal peptide.; hypothetical protein 66349 4606186 azo0058 Azoarcus sp. BH72 hypothetical protein YP_931563.1 65168 D 62928 CDS YP_931564.1 119896351 4606187 complement(66440..71053) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc3030 of R. solanacearum of 48% (sprot|YU30_RALSO) Pfam: Alpha-2-macroglobulin family Nterminal region (PF01835, PF07703) signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 71053 4606187 azo0059 Azoarcus sp. BH72 hypothetical protein YP_931564.1 66440 R 62928 CDS YP_931565.1 119896352 4606188 complement(71064..71747) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS04726 of R. solanacearum of 55% (trembl|Q8XV01(SRS)) Has PF06672;(IPR009558)Protein of unknown function (DUF1175):This family consists of several hypothetical bacterial proteins of around 210 residues in length. The function of this family is unknown. No TMHs signal peptide present.; Conserved hypothetical protein; hypothetical protein 71747 4606188 azo0060 Azoarcus sp. BH72 hypothetical protein YP_931565.1 71064 R 62928 CDS YP_931566.1 119896353 4606189 complement(71744..73438) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS04725 of Ralstonia solanacearum of 43% (trembl|Q8XV00(SRS)) No domains predicted. Signal Peptide Present. NO TMH reported present.; Conserved hypothetical protein; hypothetical protein 73438 4606189 azo0061 Azoarcus sp. BH72 hypothetical protein YP_931566.1 71744 R 62928 CDS YP_931567.1 119896354 4606190 73627..75072 1 NC_008702.1 Conserved hypothetical protein. Homology to MCA0287 of Methylococcus capsulatus of 48% (trembl:Q60C24). no domains predicted. no signal peptide. no TMHs; hypothetical protein 75072 4606190 azo0062 Azoarcus sp. BH72 hypothetical protein YP_931567.1 73627 D 62928 CDS YP_931568.1 119896355 4606191 75089..75535 1 NC_008702.1 Hypothetical protein. No good homology to the data bank. No domains predicted. No TMHs Probable signal peptide.; hypothetical protein 75535 4606191 azo0063 Azoarcus sp. BH72 hypothetical protein YP_931568.1 75089 D 62928 CDS YP_931569.1 119896356 4606192 complement(75546..76649) 1 NC_008702.1 Hypothetical protein ydiK.Integral membrane protein (potential). similarity:belongs to the upf0118 (perm) family TREMBL:Q7WQ94: 68% identity; 81% similarity. PIR:AD2790; AD2790.C97569; C97569. InterPro:IPR000585; Hemopexin. IPR002549; UPF0118. Pfam: PF01594; UPF0118; 2A0604s01: protein-export membrane pro Pfam: presence of DUF20 domain and branched chain amino acid transport. TIGRFAM: general nitrate transport. Signal P predicted signal peptide and TMHMM predicted transmembrane helices.; Function unclear; permease 76649 4606192 azo0064 Azoarcus sp. BH72 permease YP_931569.1 75546 R 62928 CDS YP_931570.1 119896357 4606193 complement(76751..77287) 1 NC_008702.1 64% similarity with ABC-type multidrug transport system, ATPase and permease components [Dechloromonas aromatica RCB]. and; hypothetical protein 77287 4606193 azo0065 Azoarcus sp. BH72 hypothetical protein YP_931570.1 76751 R 62928 CDS YP_931571.1 119896358 4606194 complement(77284..78402) 1 NC_008702.1 Enoyl-CoA hydratase and 3-2trans-enoyl-CoA isomerase are two enzymes involved in fatty acid metabolism. ECH catalyzes the hydratation of 2-trans-enoyl-CoA into 3-hydroxyacyl-CoA and ECI shifts the 3- double bond of the intermediates of unsaturated fatty acid oxidation to the 2-trans position.In Escherichia coli (gene fadB) and Pseudomonas fragi (gene faoA), ECH and ECI are also part of a multifunctional enzyme which contains both a HCDH and a 3-hydroxybutyryl-CoA epimerase domain. InterPro: Enoyl-CoA hydratase/isomerase Entry name TREMBL:Q8PMW0 Prim. accession # Q8PMW0 InterPro IPR001753; EnCoA_hydrtse. Pfam PF00378; ECH; 1 Identities = 169/379 (44%) Number of predicted TMHs: 0 Signal peptide probability: 0.000; Family membership; enoyl-CoA hydratase/isomerase 78402 4606194 azo0066 Azoarcus sp. BH72 enoyl-CoA hydratase/isomerase YP_931571.1 77284 R 62928 CDS YP_931572.1 119896359 4606195 78461..79156 1 NC_008702.1 DNA polymerase III polC-type (EC 2.7.7.7) (PolIII). Required for replicative DNA synthesis. This DNA polymerase also exhibits 3 to 5 exonuclease activity (By similarity). InterPro: Exonuclease dnaq: DNA polymerase III epsilon subun; High confidence in function and specificity; DNA-directed DNA polymerase 79156 polC 4606195 polC Azoarcus sp. BH72 DNA-directed DNA polymerase YP_931572.1 78461 D 62928 CDS YP_931573.1 119896360 4609597 complement(79164..79916) 1 NC_008702.1 Hypothetical protein. No good homology with any hits of this length protein. No Domains/Features/motifs/signal peptide present.; hypothetical protein 79916 4609597 azo0068 Azoarcus sp. BH72 hypothetical protein YP_931573.1 79164 R 62928 CDS YP_931574.1 119896361 4606196 80013..80393 1 NC_008702.1 Conserved hypothetical globin-like protein. Homology to rpa2719 of R. palustris of 46% (tremblnew|CAE28161). Interpro: IPR009050 Globin-like. Globins are heme-containing proteins involved in binding and/or transporting oxygen. This family of heme binding proteins are found mainly in bacteria. No signal peptide. No TMHs; Function unclear; globin-like protein 80393 4606196 azo0069 Azoarcus sp. BH72 globin-like protein YP_931574.1 80013 D 62928 CDS YP_931575.1 119896362 4606197 complement(80397..80645) 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted.signal peptide. 1 TMHs; hypothetical protein 80645 4606197 azo0070 Azoarcus sp. BH72 hypothetical protein YP_931575.1 80397 R 62928 CDS YP_931576.1 119896363 4606198 80784..82139 1 NC_008702.1 Sigma-54 dependent response regulator,; Specificity unclear; sigma-54 dependent response regulator 82139 4606198 azo0071 Azoarcus sp. BH72 sigma-54 dependent response regulator YP_931576.1 80784 D 62928 CDS YP_931577.1 119896364 4606199 82158..84344 1 NC_008702.1 Putative two component sensor histidine kinase,; Specificity unclear; putative sensor histidine kinase 84344 4606199 azo0072 Azoarcus sp. BH72 putative sensor histidine kinase YP_931577.1 82158 D 62928 CDS YP_931578.1 119896365 4606200 84372..85583 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. Probable sugar-proton symporter transmembrane protein ProP proline/betaine transporter. Low homology to the hypothetical protein ycxA (ORF5)involved in the biosynthesis of the pepitde antibiotic Bacitracin in B. subtilis. Signal peptide; Specificity unclear; putative MFS permease 85583 4606200 azo0073 Azoarcus sp. BH72 putative MFS permease YP_931578.1 84372 D 62928 CDS YP_931579.1 119896366 4606201 85691..87382 1 NC_008702.1 Hypothetical protein yfbK. The von Willebrand factor is a large multimeric glycoprotein found in blood plasma. Mutant forms are involved in the aetiology of bleeding disorders [1]. In von Willebrand factor, the type A domain (vWF) is the prototype for a protein superfamily. trembl:Q8P6Q2: 55% identity; 66% similarity InterPro:IPR002016; Peroxidase. IPR002035; VWF_A. Pfam: PF00092; VWA; 1. SMART: SM00327; VWA; 1. cdhD: CO dehydrogenase/acetyl-CoA syn Signal peptide present (0.927 probability); SignalP predicted. No transmembrane helices (TMHMM predicted).; Function unclear; hypothetical protein 87382 4606201 azo0074 Azoarcus sp. BH72 hypothetical protein YP_931579.1 85691 D 62928 CDS YP_931580.1 119896367 4606202 87379..87978 1 NC_008702.1 Bacteria have multiple sigma factors which are active under specific conditions; the sigma factor binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; RNA polymerase sigma factor 87978 rpoE 4606202 rpoE Azoarcus sp. BH72 RNA polymerase sigma factor YP_931580.1 87379 D 62928 CDS YP_931581.1 119896368 4609798 87975..88751 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BB3269 of Bordetella bronchiseptica of 31% (trembl|Q7WHD9(SRS)). No domains predicted. no signal peptide 1 TMH; Conserved hypothetical protein; hypothetical protein 88751 4609798 azo0076 Azoarcus sp. BH72 hypothetical protein YP_931581.1 87975 D 62928 CDS YP_931582.1 119896369 4606203 complement(88772..88993) 1 NC_008702.1 Hypothetical protein. No Good homologs. No Domains/Features/Motifs reported present.; hypothetical protein 88993 4606203 azo0077 Azoarcus sp. BH72 hypothetical protein YP_931582.1 88772 R 62928 CDS YP_931583.1 119896370 4606204 complement(89004..89966) 1 NC_008702.1 Hypothetical protein. Weak homology with hits over entire length. PS50821; PAZ domain present(Prosite); hypothetical protein 89966 4606204 azo0078 Azoarcus sp. BH72 hypothetical protein YP_931583.1 89004 R 62928 CDS YP_931584.1 119896371 4606205 complement(90144..91352) 1 NC_008702.1 rtcB protein, 57% identical(70% similairty) to TrEMBL;Q7NZ85 TrEMBL;Q8FCS7(56% identity). Has IPR001233;UPF0027(PF01139, Uncharacterized protein family):A number of uncharacterized proteins including Escherichia coli rtcB, Mycobacterium tuberculosis MtCY441.01., Caenorhabditis elegans F16A11.2 and Methanococcus jannaschii MJ0682 belong to this family. Has no Signal peptide or TMH present.; High confidence in function and specificity; rtcB protein 91352 rtcB 4606205 rtcB Azoarcus sp. BH72 rtcB protein YP_931584.1 90144 R 62928 CDS YP_931585.1 119896372 4609831 91812..93434 1 NC_008702.1 stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein; peptide chain release factor 3 93434 prfC 4609831 prfC Azoarcus sp. BH72 peptide chain release factor 3 YP_931585.1 91812 D 62928 CDS YP_931586.1 119896373 4609625 93454..93981 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to pa4965 ao P. aeruginosa of 50% (trembl|Q9HUK0) Pfam: Retroviral aspartyl protease no signal peptide 1 TMHs; Conserved hypothetical protein; hypothetical protein 93981 4609625 azo0081 Azoarcus sp. BH72 hypothetical protein YP_931586.1 93454 D 62928 CDS YP_931587.1 119896374 4606206 complement(94431..95405) 1 NC_008702.1 Required for efficient pilin antigenic variation; recombination associated protein 95405 rdgC 4606206 rdgC Azoarcus sp. BH72 recombination associated protein YP_931587.1 94431 R 62928 CDS YP_931588.1 119896375 4609699 complement(95476..95943) 1 NC_008702.1 hypothetical protein 95943 4609699 azo0083 Azoarcus sp. BH72 hypothetical protein YP_931588.1 95476 R 62928 CDS YP_931589.1 119896376 4606207 96022..96768 1 NC_008702.1 Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70) (16S pseudouridylate 516 synthase) (16S pseudouridine 516 synthase) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA.; Family membership; pseudouridylate synthase 96768 rsuA 4606207 rsuA Azoarcus sp. BH72 pseudouridylate synthase YP_931589.1 96022 D 62928 CDS YP_931590.1 119896377 4609830 97020..98186 1 NC_008702.1 Conserved hypothetical protein. Homology to RS04117 of R.solanacearum of 52% (tremble:Q8Y0H4) No domains predicted. No signal peptide. No TMHs; hypothetical protein 98186 4609830 azo0085 Azoarcus sp. BH72 hypothetical protein YP_931590.1 97020 D 62928 CDS YP_931591.1 119896378 4606208 98247..98804 1 NC_008702.1 Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA; elongation factor P 98804 efp 4606208 efp Azoarcus sp. BH72 elongation factor P YP_931591.1 98247 D 62928 CDS YP_931592.1 119896379 4608662 98928..99359 1 NC_008702.1 Hypothetical secreted protein. Homology to RS00193 of R. solanacearum of 26% (tremble:Q8XVC9) PF02987, Late embryogenesis abundant protein;IPR004238;Different types of LEA proteins are expressed at different stages of late embryogenesis in higher plant seed embryos and under conditions of dehydration stress. The function of these proteins is unknown. No TMHs Signal peptide present; hypothetical protein 99359 4608662 azo0087 Azoarcus sp. BH72 hypothetical protein YP_931592.1 98928 D 62928 CDS YP_931593.1 119896380 4606209 99435..105044 1 NC_008702.1 UvrABC system protein A (UvrA protein) (Excinuclease ABC subunit A). The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB the uvrA molecules dissociate (By similarity). InterPro: Excinuclease ABC A subunit; High confidence in function and specificity; putative excinuclease ABC subunit 105044 uvrA1 4606209 uvrA1 Azoarcus sp. BH72 putative excinuclease ABC subunit YP_931593.1 99435 D 62928 CDS YP_931594.1 119896381 4610080 105090..105788 1 NC_008702.1 Conserved hypothetical hydrogenase cytochrome b-type subunit. Homology to C. vinosum of 56% (trembl|Q46471). Involved in electron transfer from hydrogen to oxygen. probable signal peptide probable 4 TMHs; Family membership; hydrogenase cytochrome b-type subunit 105788 4610080 azo0089 Azoarcus sp. BH72 hydrogenase cytochrome b-type subunit YP_931594.1 105090 D 62928 CDS YP_931595.1 119896382 4606210 complement(105850..106314) 1 NC_008702.1 Probable cytochrome c'. Homology to cycA of C. vinosum of 61% (sprot|CYCP_CHRVI). CYTOCHROME C IS THE MOST WIDELY OCCURRING BACTERIAL C-TYPE CYTOCHROME. CYTOCHROMES C ARE HIGH-SPIN PROTEINS AND THE HEME HAS NO SIXTH LIGAND. THEIR EXACT FUNCTION IS NOT KNOWN. Pfam: Cytochrome c. signal peptide no TMHs; Function unclear; cytochrome c' 106314 cycA1 4606210 cycA1 Azoarcus sp. BH72 cytochrome c' YP_931595.1 105850 R 62928 CDS YP_931596.1 119896383 4608548 106574..108199 1 NC_008702.1 Methyl-accepting chemotaxis protein,; Specificity unclear; methyl-accepting chemotaxis protein 108199 4608548 azo0091 Azoarcus sp. BH72 methyl-accepting chemotaxis protein YP_931596.1 106574 D 62928 CDS YP_931597.1 119896384 4606211 complement(108207..109646) 1 NC_008702.1 Probable serine protease. Homology to mucD of P. aeruginosa of 55% (AAC43718). Pfam: Trypsin; PDZ domain (also known as DHR or GLGF) signal peptide no TMHs; High confidence in function and specificity; serine protease MucD 109646 mucD1 4606211 mucD1 Azoarcus sp. BH72 serine protease MucD YP_931597.1 108207 R 62928 CDS YP_931598.1 119896385 4609283 complement(109778..111763) 1 NC_008702.1 23% TonB_boxC. Pfam:PF00593; TonB_dep_Rec; 1.; High confidence in function and specificity; putative TonB-dependent receptor 111763 irgA1 4609283 irgA1 Azoarcus sp. BH72 putative TonB-dependent receptor YP_931598.1 109778 R 62928 CDS YP_931599.1 119896386 4609087 111975..112913 1 NC_008702.1 oxyR, Hydrogen peroxide-inducible genes activator. BELONGS TO THE LYSR FAMILY OF TRANSCRIPTIONAL REGULATORS REQUIRED FOR THE INDUCTION OF A REGULON OF HYDROGEN PEROXIDE INDUCIBLE GENES SUCH AS CATALASE GLUTATHIONE-REDUCTASE ETC. (BY SIMILARITY). mreB: cell shape determining protein M 51% HTH_LysR. IPR005119; LysR_subst. IPR009058; Wing_hlx_DNA_bnd. PF00126; HTH_1; 1. PF03466; LysR_substrate; 1. HTH reporting nucleic acid binding motif; High confidence in function and specificity; putative oxyR 112913 oxyR 4609087 oxyR Azoarcus sp. BH72 putative oxyR YP_931599.1 111975 D 62928 CDS YP_931600.1 119896387 4609464 complement(112981..115554) 1 NC_008702.1 decatenates replicating daughter chromosomes; DNA topoisomerase III 115554 4609464 azo0095 Azoarcus sp. BH72 DNA topoisomerase III YP_931600.1 112981 R 62928 CDS YP_931601.1 119896388 4606212 complement(115666..116133) 1 NC_008702.1 Protein smg homolog, 45% identity (65% similarity) to SwissProt;P30853 43% identity to SwissProt;Q8X8F6,E.coli smg protein. Has PF04361(IPR007456):Protein of unknown function (DUF494);Members of this family of uncharacterised proteins are often named Smg. No signal peptide or TMH reported present.; hypothetical protein 116133 4606212 azo0096 Azoarcus sp. BH72 hypothetical protein YP_931601.1 115666 R 62928 CDS YP_931602.1 119896389 4606213 complement(116194..117312) 1 NC_008702.1 Putative DNA processing protein DrpA (Smf protein). Homology to drpA of H. influcenzae The SMF family (DNA processing chain A, dprA) are a group of bacterial proteins. In Helicobacter pylori, dprA is required for natural chromosomal and plasmid transformation InterPro: SMF family Tigerfam: dprA: DNA processing protein DprA,putative Pfam SMF family no signal peptide no TMHs; Family membership; putative DNA processing protein DrpA 117312 drpA 4606213 drpA Azoarcus sp. BH72 putative DNA processing protein DrpA YP_931602.1 116194 R 62928 CDS YP_931603.1 119896390 4608652 complement(117316..118503) 1 NC_008702.1 Conseved hypothetical membrane protein. Homology to RSO2271 of R. solanacearum of 36% (trembl|Q8Y3B1(SRS)). Pfam: Lysin motif (SM00257) This domain is about 40 residues long and is found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function. No signal peptide 1 TMH; Conserved hypothetical protein; hypothetical protein 118503 4608652 azo0098 Azoarcus sp. BH72 hypothetical protein YP_931603.1 117316 R 62928 CDS YP_931604.1 119896391 4606214 118530..119033 1 NC_008702.1 cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+); peptide deformylase 119033 def 4606214 def Azoarcus sp. BH72 peptide deformylase YP_931604.1 118530 D 62928 CDS YP_931605.1 119896392 4608616 119079..120044 1 NC_008702.1 modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) which is important in translation initiation; inactivation of this gene in Escherichia coli severely impairs growth; methionyl-tRNA formyltransferase 120044 fmt 4608616 fmt Azoarcus sp. BH72 methionyl-tRNA formyltransferase YP_931605.1 119079 D 62928 CDS YP_931606.1 119896393 4608815 120104..120946 1 NC_008702.1 Putative protease HtpX Homolog to htpX of E. coli of 30% (AAA62779) InterPro: Peptidase family M48 (IPR001915) Pfam: Peptidase family M48 signal peptide TMHs3; Family membership; M48 family peptidase 120946 htpX 4608815 htpX Azoarcus sp. BH72 M48 family peptidase YP_931606.1 120104 D 62928 CDS YP_931607.1 119896394 4609040 120960..121487 1 NC_008702.1 Putative thiol:disulfide oxidoreductase. Homology to helX of R. casulata of 35% Periplasmic protein thiol:disulphide oxidoreductase is involved in the biogenesis of c-type cytochromes as well as in disulphide bond formation in some periplasmic proteins. Tigrfam: dsbE: periplasmic protein thiol:disulfide signal peptide no TMHs; High confidence in function and specificity; putative thiol:disulfide oxidoreductase 121487 helX 4609040 helX Azoarcus sp. BH72 putative thiol:disulfide oxidoreductase YP_931607.1 120960 D 62928 CDS YP_931608.1 119896395 4608979 121484..122596 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to SMa0025 of S. meliloti of 46% pir|D95263(SRS). Pfam: Transglutaminase_like Superfamily signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 122596 4608979 azo0103 Azoarcus sp. BH72 hypothetical protein YP_931608.1 121484 D 62928 CDS YP_931609.1 119896396 4606215 122689..123570 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA582 of Azoacrus sp EbN1 (trembl:Q5P8D6). No domains predicted. SignalP reporting signal peptid. no TMHs; Conserved hypothetical protein; hypothetical protein 123570 4606215 azo0104 Azoarcus sp. BH72 hypothetical protein YP_931609.1 122689 D 62928 CDS YP_931610.1 119896397 4606216 123626..124126 1 NC_008702.1 Probable thiol peroxidase. Homology to E. coli of 62% (sprot:TPX_ECOLI) Has antioxidant activity. Could remove peroxides or H(2)O(2) (By similarity). Pfam: AhpC/TSA family no signal peptide no TMHs; High confidence in function and specificity; thiol peroxidase 124126 tpx 4606216 tpx Azoarcus sp. BH72 thiol peroxidase YP_931610.1 123626 D 62928 CDS YP_931611.1 119896398 4609965 124218..125105 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to an orf of R. palustris (tremblnew|CAE28417). Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Tranporter InterPro: Integral membrane protein DUF6 Pfam: Integral membrane protein DUF6 no signal peptide probable 10 TMHs; Conserved hypothetical protein; hypothetical protein 125105 4609965 azo0106 Azoarcus sp. BH72 hypothetical protein YP_931611.1 124218 D 62928 CDS YP_931612.1 119896399 4606217 complement(125118..125834) 1 NC_008702.1 conserved hypothetical membrane proteins, 42% identity (59% similarity) to TrEMBL;Q73PF0 Has PF02592;Uncharacterized ACR, YhhQ family COG1738;IPR003744:This is a family of uncharacterized proteins. Conserved regions of hydrophobicity suggest that all members of the family may be integral membrane proteins. no signal peptide. 4 TMH.; Conserved hypothetical protein; hypothetical protein 125834 4606217 azo0107 Azoarcus sp. BH72 hypothetical protein YP_931612.1 125118 R 62928 CDS YP_931613.1 119896400 4606218 126088..127293 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to XCC0740 of Xanthomonas campestris of 33% (trembl|Q8PCI6(SRS) Pfam: Glycosyl hydrolases family 2,TIM barrel domain This family contains beta-galactosidase,beta-mannosidase and beta-glucuronidase activities. No TMHs Signal peptide: present.; Conserved hypothetical protein; hypothetical protein 127293 4606218 azo0108 Azoarcus sp. BH72 hypothetical protein YP_931613.1 126088 D 62928 CDS YP_931614.1 119896401 4606219 127357..127812 1 NC_008702.1 hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); possible defense mechanism against a harmful effect of D-tyrosine; D-tyrosyl-tRNA(Tyr) deacylase 127812 yihZ 4606219 yihZ Azoarcus sp. BH72 D-tyrosyl-tRNA(Tyr) deacylase YP_931614.1 127357 D 62928 CDS YP_931615.1 119896402 4610186 complement(127825..128703) 1 NC_008702.1 Pirin-like protein. TREMBL:Q8P799: 52% identity,65% similarity. InterPro: DUF209 his region is found the C-terminal half of the Pirin protein. The function of Pirin is unknown but the gene coding for this protein is known to be expressed in all tissues in the human body although it is expressed most strongly in the liver and heart. Pirin is known to be a nuclear protein, exclusively localised within the nucleoplasma and predominantly concentrated within dot-like subnuclear structures. A tomato homologue of human Pirin has been found to be induced during programmed cell death. Human Pirin interacts with Bcl-3 and NFI and hence is probably involved in the regulation of DNA transcription and replication. It appears to be an Fe(II)-containing member of the Cupin superfamil InterPro:IPR007113; Cupin_sup. IPR008778; Pirin_C. IPR003829; Pirin_N. Pfam PF02678; Pirin; 1. PF05726; Pirin_C molyb_syn: molybdenum cofactor synthesis Absence of transmembrane helices (TMHMM predicted); Function unclear; pirin-related protein 128703 4610186 azo0110 Azoarcus sp. BH72 pirin-related protein YP_931615.1 127825 R 62928 CDS YP_931616.1 119896403 4606220 128838..129824 1 NC_008702.1 Putative Alcohol dehydrogenase. Homology to adh-HT of B. stearothermophilus (sprot|ADH3_BACST) THERMOSTABLE AND THERMOPHILIC NAD(+)-DEPENDENT ALCOHOL DEHYDROGENASE. BEARS MAINLY AN ETHANOL-DEHYDROGENASE ACTIVITY. CATALYTIC ACTIVITY:An alcohol + NAD(+) = an aldehyde or ketone + NADH. COFACTOR: Binds 2 zinc ions per subunit (By similarity). Pfam: Zinc-binding dehydrogenase Tirgfam: tdh: L-threonine 3-dehydrogenase no signal peptide no TMHs; Family membership; putative alcohol dehydrogenase 129824 adhA 4606220 adhA Azoarcus sp. BH72 putative alcohol dehydrogenase YP_931616.1 128838 D 62928 CDS YP_931617.1 119896404 4608279 complement(129841..130737) 1 NC_008702.1 Putative transcriptional regulator. Homology to gstR of R. leguminosarum of 37% (tremble:Q52827). Has PF00126, HTH_1,Bacterial regulatory helix-turn-helix protein, lysR family.IPR000847, HTH_LysR; Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif. These proteins are very diverse, but for convenience may be grouped into subfamilies on the basis of sequence similarity. One such family, the lysR family, groups together a range of proteins, including ampR, catM, catR, cynR, cysB, gltC,iciA, ilvY, irgB, lysR, metR, mkaC, mleR, nahR, nhaR,nodD, nolR, oxyR, pssR, rbcR, syrM, tcbR, tfdS and trpI. The majority of these proteins appear to be transcription activators and most are known to negatively regulate their own expression. All possess a potential HTH DNA-binding motif towards their N-termini. Has PF03466, LysR substrate binding domain;IPR005119, LysR_subst; The structure of this domain is known and is; High confidence in function and specificity; putative transcriptional regulator 130737 gstR1 4608279 gstR1 Azoarcus sp. BH72 putative transcriptional regulator YP_931617.1 129841 R 62928 CDS YP_931618.1 119896405 4608959 130842..131489 1 NC_008702.1 Probabe glutathione transferase. Homology to gstA of R. leguminosarum of 58% (sprot:GSTA_RHILE) Conjugation of reduced glutathione to a variety of targets. Pfam: PF02798, GST_N, Glutathione S-transferase, N-terminal domain; PF00043, GST_C, Glutathione S-transferase, C-terminal domain Interpro: IPR004045, GST_Nterm; IPR004046,GST_Cterm; no signal peptide no TMHs; High confidence in function and specificity; glutathione transferase 131489 gstA 4608959 gstA Azoarcus sp. BH72 glutathione transferase YP_931618.1 130842 D 62928 CDS YP_931619.1 119896406 4608957 131492..132490 1 NC_008702.1 Conserved hypothetical protein. Homology to bp2124 of B. pertussis of 48% (trembl|Q7VWS5). Pfam: Pyridoxamine 5-phosphate oxidase no signal peptide no TMHs; hypothetical protein 132490 4608957 azo0114 Azoarcus sp. BH72 hypothetical protein YP_931619.1 131492 D 62928 CDS YP_931620.1 119896407 4606221 complement(132910..133359) 1 NC_008702.1 MaoC protein (Phenylacetic acid degradation protein paaZ). Entry name :- TREMBL:Q8XXS0 Prim. accession # Q8XXS0 InterPro:-IPR002539; MaoC_dehydratas. Pfam:-PF01575; MaoC_dehydratas; 1. Identities = 77/145 (53%) Number of predicted TMHs: 0; hypothetical protein 133359 4606221 azo0115 Azoarcus sp. BH72 hypothetical protein YP_931620.1 132910 R 62928 CDS YP_931621.1 119896408 4606222 complement(133377..134873) 1 NC_008702.1 Catalyzes the hydrolysis of AMP to form adenine and ribose 5-phosphate using water as the nucleophile; AMP nucleosidase 134873 amn 4606222 amn Azoarcus sp. BH72 AMP nucleosidase YP_931621.1 133377 R 62928 CDS YP_931622.1 119896409 4608311 135086..135853 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XU49 (72%) and to sprot|BRAF_PSEAE (40%). Pfam: ABC transporter Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 135853 braF 4608311 braF Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931622.1 135086 D 62928 CDS YP_931623.1 119896410 4608405 135870..136592 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q7W5F6 (63%) and to trembl|Q8U8G7 (59%). Pfam (PF00005): ABC transporter Smart (SM00382): ATPase superfamily; Specificity unclear; ABC transporter ATP-binding protein 136592 4608405 azo0118 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931623.1 135870 D 62928 CDS YP_931624.1 119896411 4606223 136627..137841 1 NC_008702.1 ABC transporter substrate binding proteins count to the family of extracellular ligand binding proteins. It is a component of the high affinity amino acid transport system. Similar to trembl|Q8XU51 (58%) and to tremblnew|CAE27314 (45%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; ABC transporter substrate-binding protein 137841 4606223 azo0119 Azoarcus sp. BH72 ABC transporter substrate-binding protein YP_931624.1 136627 D 62928 CDS YP_931625.1 119896412 4606224 137898..138782 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q92LQ5 (71%) and to trembl|Q8XU52 (69%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting eight Tmhelix.; Specificity unclear; ABC transporter permease 138782 4606224 azo0120 Azoarcus sp. BH72 ABC transporter permease YP_931625.1 137898 D 62928 CDS YP_931626.1 119896413 4606225 138807..139763 1 NC_008702.1 Putative branched-chain amino acid transport permease. Homology to livM of S. typhimurium of 25%. Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. Pfam: branch-chain amino acid transport system probable signal peptide probable 6 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 139763 livM1 4606225 livM1 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_931626.1 138807 D 62928 CDS YP_931627.1 119896414 4609158 complement(139919..141091) 1 NC_008702.1 23S rRNA m2A2503 methyltransferase; methylates the C2 position of the A2530 nucleotide in 23S rRNA; may be involved in antibiotic resistance; ribosomal RNA large subunit methyltransferase N 141091 4609158 azo0122 Azoarcus sp. BH72 ribosomal RNA large subunit methyltransferase N YP_931627.1 139919 R 62928 CDS YP_931628.1 119896415 4606226 141038..141463 1 NC_008702.1 putative threonine efflux protein 141463 4606226 azo0123 Azoarcus sp. BH72 putative threonine efflux protein YP_931628.1 141038 D 62928 CDS YP_931629.1 119896416 4606227 141533..141742 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted signal peptide no TMHs; hypothetical protein 141742 4606227 azo0124 Azoarcus sp. BH72 hypothetical protein YP_931629.1 141533 D 62928 CDS YP_931630.1 119896417 4606228 141717..142061 1 NC_008702.1 Conserved hypothetical secreted protein,; Conserved hypothetical protein; hypothetical protein 142061 4606228 azo0125 Azoarcus sp. BH72 hypothetical protein YP_931630.1 141717 D 62928 CDS YP_931631.1 119896418 4606229 142061..142369 1 NC_008702.1 Conserved hypothetical protein with 74% similarity(57% identity)to Conserved hypothetical protein [Sinorhizobium meliloti]. No Signal peptide reported to be present. No TMH reported to be present. Has PF07045,Protein of unknown function (DUF1330)This family consists of several hypothetical bacterial proteins of around 90 residues in length. The function of this family is unknown. TIGRFAM:acolac_sm: acetolactate synthase,small s; Family membership; hypothetical protein 142369 4606229 azo0126 Azoarcus sp. BH72 hypothetical protein YP_931631.1 142061 D 62928 CDS YP_931632.1 119896419 4606230 142468..142659 1 NC_008702.1 Hypothetical protein, 40% identity to TrEMBL;Q73S36 Weak Homology with most protein hits in the database. No Domains/Features/Motifs predicted present.; hypothetical protein 142659 4606230 azo0127 Azoarcus sp. BH72 hypothetical protein YP_931632.1 142468 D 62928 CDS YP_931633.1 119896420 4606231 complement(142690..143106) 1 NC_008702.1 Conserved hypothetical membrane protein, 50% identity (62% similarity) to TrEMBL;Q7NQ67. No domains predicted TMHMM2 reporting 1 TMH's present. No signal peptide reported present.; Conserved hypothetical protein; hypothetical protein 143106 4606231 azo0128 Azoarcus sp. BH72 hypothetical protein YP_931633.1 142690 R 62928 CDS YP_931634.1 119896421 4606232 143294..144907 1 NC_008702.1 The Tap (taxis toward peptides) receptor and the periplasmic dipeptide-binding protein (DBP) of Escherichia coli together mediate chemotactic responses to dipeptides. Similar trembl|Q7P0E0 (32%) and to SWISSPROT:MCPC_SALTY (23%). Pfam(PF00015): Bacterial chemotaxis sensory transducer Pfam (PF00672):HAMP domain TMHMM reporting two TMH. SignalP reporting Signalpeptid.; Specificity unclear; putative dipeptide chemoreceptor 144907 tap 4606232 tap Azoarcus sp. BH72 putative dipeptide chemoreceptor YP_931634.1 143294 D 62928 CDS YP_931635.1 119896422 4609915 145152..146765 1 NC_008702.1 The ability of bacteria to recognize and swim toward aromatic hydrocarbons is possibly an important prelude to their degradation. In Pseudomonas putida G7,NahY is the receptor required for the chemotaxis to naphthalene. Similar to trembl|Q7NU44 (38%) and to trembl|Q7NY58 (33%). Smart: HAMP domain Pfam (PF00015): Methyl-accepting chemotaxis protein (MCP) signalling domain SignalP reporting Signal Peptide.; Specificity unclear; putative aromatic hydrocarbon chemotaxis transducer 146765 nahY 4609915 nahY Azoarcus sp. BH72 putative aromatic hydrocarbon chemotaxis transducer YP_931635.1 145152 D 62928 CDS YP_931636.1 119896423 4609326 146889..147092 1 NC_008702.1 Probable tautomerase lin2709 (EC 5.3.2.2). TREMBL:Q7UE84: 67% identity, 86% similarity InterPro:IPR004370; Taut. ProDom: PD404143 InterPro: 4-oxalocrotonate tautomerase taut: 4-oxalocrotonate tautomerase No signal peptide no TMH's; High confidence in function and specificity; 4-oxalocrotonate tautomerase 147092 dmpI 4609326 dmpI Azoarcus sp. BH72 4-oxalocrotonate tautomerase YP_931636.1 146889 D 62928 CDS YP_931637.1 119896424 4608628 147273..148154 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 148154 4608628 azo0132 Azoarcus sp. BH72 LysR family transcriptional regulator YP_931637.1 147273 D 62928 CDS YP_931638.1 119896425 4606233 148401..149321 1 NC_008702.1 Branched-chain amino acid aminotransferase (EC 2.6.1.42) (BCAT). Acts on leucine isoleucine and valine (By similarity). InterPro: Aminotransferases class-IV; High confidence in function and specificity; branched-chain-amino-acid transaminase 149321 ilvE1 4606233 ilvE1 Azoarcus sp. BH72 branched-chain-amino-acid transaminase YP_931638.1 148401 D 62928 CDS YP_931639.1 119896426 4609072 complement(149318..150751) 1 NC_008702.1 Hypothetical response regulator, TREMBL: trembl|Q7MSI2 (24% Wolinella succinogenes, hypothetical protein ws0414) InterPro: IPR001610 PAC. IPR000014 PAS. IPR001789 Response_reg. IPR000160 GGDEF. Pfam: PF00990 GGDEF domain. PF00072 Response_reg. PF00785 PAC. PF00989 PAS. TIGRFAM: TIGR00254 putative diguanylate cyclase (GGDEF) domain. TIGR00229 PAS domain S-box.; Conserved hypothetical protein; putative response regulator 150751 ydaM 4609072 ydaM Azoarcus sp. BH72 putative response regulator YP_931639.1 149318 R 62928 CDS YP_931640.1 119896427 4610163 complement(150748..151149) 1 NC_008702.1 Putative response regulator; putative response regulator 151149 4610163 azo0135 Azoarcus sp. BH72 putative response regulator YP_931640.1 150748 R 62928 CDS YP_931641.1 119896428 4606234 complement(151184..153406) 1 NC_008702.1 Putative hybrid sensor and regulator protein; putative hybrid sensor and regulator protein 153406 4606234 azo0136 Azoarcus sp. BH72 putative hybrid sensor and regulator protein YP_931641.1 151184 R 62928 CDS YP_931642.1 119896429 4606235 complement(153403..154701) 1 NC_008702.1 Aliphatic amidase expression-regulating protein,AmiC regulates the expression of the inducible aliphatic amidase activity in Pseudomonas aeruginosa. Similar to sprot|AMIC_PSEAE (31%) and to trembl|P74390 (50%).Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative aliphatic amidase expression-regulating protein 154701 amiC1 4606235 amiC1 Azoarcus sp. BH72 putative aliphatic amidase expression-regulating protein YP_931642.1 153403 R 62928 CDS YP_931643.1 119896430 4608308 154860..155846 1 NC_008702.1 Conserved hypothetical protein.Similarity to b.subtilis bmru and to synechocystis pcc 6803 sll0036. Identities = 29/85 (34%) Entry name SWISSPROT:YEGS_ECOLI Prim. accession # P76407 InterPro IPR005218; Cons_hypoth147. IPR001206; DAGKc. Pfam PF00781; DAGKc; 1. Prediction: Non-secretory protein Signal peptide probability: 0.00 Number of predicted TMHs: 0; hypothetical protein 155846 4608308 azo0138 Azoarcus sp. BH72 hypothetical protein YP_931643.1 154860 D 62928 CDS YP_931644.1 119896431 4606236 155860..156708 1 NC_008702.1 Serine/threonine (S/T) phosphatases catalyse the dephosphorylation of phosphoserine and phosphothreonine residues. In mammalian tissues four different types of PP have been identified and are known as PP1, PP2A, PP2B and PP2C. Except for PP2C, these enzymes are evolutionary related. The catalytic regions of the proteins are well conserved TREMBL:Q7VZQ9: 41% identity, 50% similarity InterPro:IPR004843; M-ppestrase. Pfam:PF00149; Metallophos InterPro: Serine/threonine specific protein phosphatase No transmembrane helices sbcd: exonuclease SbcD; High confidence in function and specificity; phosphodiesterase 156708 cpdA 4606236 cpdA Azoarcus sp. BH72 phosphodiesterase YP_931644.1 155860 D 62928 CDS YP_931645.1 119896432 4608525 complement(156692..157861) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Ralstonia eutropha of 61% (ZP_00167955). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 157861 4608525 azo0140 Azoarcus sp. BH72 hypothetical protein YP_931645.1 156692 R 62928 CDS YP_931646.1 119896433 4606237 158045..159949 1 NC_008702.1 GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs; tRNA uridine 5-carboxymethylaminomethyl modification protein GidA 159949 gidA 4606237 gidA Azoarcus sp. BH72 tRNA uridine 5-carboxymethylaminomethyl modification protein GidA YP_931646.1 158045 D 62928 CDS YP_931647.1 119896434 4608876 159946..160584 1 NC_008702.1 glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA; 16S rRNA methyltransferase GidB 160584 gidB 4608876 gidB Azoarcus sp. BH72 16S rRNA methyltransferase GidB YP_931647.1 159946 D 62928 CDS YP_931648.1 119896435 4608877 160716..161486 1 NC_008702.1 putative chromosome partitioning protein; High confidence in function and specificity; ParA family protein 161486 parA1 4608877 parA1 Azoarcus sp. BH72 ParA family protein YP_931648.1 160716 D 62928 CDS YP_931649.1 119896436 4609497 161496..161789 1 NC_008702.1 Hypothetical protein MJ0435. trembl:Q8DGT1:51% identity, 64% similarity InterPro: IPR002934; NTP_transf. Pfam: PF01909; NTP_transf_2 No signal peptide (Signal P predicted) No transmembrane helices (TMHMM predicted). hstdl_phs_rel: histidinol phosphatase-r; Specificity unclear; nucleotidyltransferase 161789 4609497 azo0144 Azoarcus sp. BH72 nucleotidyltransferase YP_931649.1 161496 D 62928 CDS YP_931650.1 119896437 4606238 161786..162148 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to TLL2231 of Thermosynechococcus elongatus of 34% (tremble:Q8DGT2). Signal peptide present. No TMH reported present. Has (IPR008201)PF01934 Protein of unknown function DUF86;The function of members of this family is unknown.; Conserved hypothetical protein; hypothetical protein 162148 4606238 azo0145 Azoarcus sp. BH72 hypothetical protein YP_931650.1 161786 D 62928 CDS YP_931651.1 119896438 4606239 162150..163013 1 NC_008702.1 Probable chromosome partitioning protein,; High confidence in function and specificity; chromosome partitioning protein ParB 163013 parB 4606239 parB Azoarcus sp. BH72 chromosome partitioning protein ParB YP_931651.1 162150 D 62928 CDS YP_931652.1 119896439 4609500 complement(163114..165879) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS05051 of Ralstonia solanacearum of 45% (trembl|Q8Y1A4(SRS)). TMHMM2 reporting the presence of 26 TMH's. Signal P reporting Signal Peptide present. Coils2 program reporting presence of Coiled coil.; Conserved hypothetical protein; hypothetical protein 165879 4609500 azo0147 Azoarcus sp. BH72 hypothetical protein YP_931652.1 163114 R 62928 CDS YP_931653.1 119896440 4606240 complement(165901..167784) 1 NC_008702.1 Similar to the Aas bifunctional protein [includes: 2-acylglycerophosphoethanolamine acyltransferase (2-acyl-gpe acyltransferase); acyl-acyl carrier protein synthetase (acyl-acp synthetase)].Plays a role in lysophospholipid acylation. 25% Acyltransferase.IPR000873; AMP-bind. Pfam:PF01553; Acyltransferase; 1.PF00501; AMP-binding; 1. TMhelix:10; Function unclear; putative acyltransferase family protein 167784 4606240 azo0148 Azoarcus sp. BH72 putative acyltransferase family protein YP_931653.1 165901 R 62928 CDS YP_931654.1 119896441 4606241 complement(167781..169031) 1 NC_008702.1 tremblnew|AAR36422:35% identity, 46% similarity EMBL:AE017218; AAR36422.1; TIGR:GSU3030; This Fe2+-requiring enzyme plays a role in D-glucuronate catabolism in Escherichia coli. Mannonate dehydratase converts D-mannonate to 2-dehydro-3-deoxy-D-gluconate. An apparent equivalog is found in a glucuronate utilization operon in Bacillus stearothermophilus T-6. Interpro:IPR004628;Man_dehyd SignalP predicted signal peptide present. No. of transmembrane helices: 1 (TMHMM predicted) pyrC_dimer: dihydroorotase homodimeric; Specificity unclear; putative mannonate dehydratase 169031 uxuA 4606241 uxuA Azoarcus sp. BH72 putative mannonate dehydratase YP_931654.1 167781 R 62928 CDS YP_931655.1 119896442 4610086 complement(169028..171166) 1 NC_008702.1 AAS bifunctional protein [Includes: 2-acylglycerophosphoethanolamine acyltransferase (2-acyl-GPE acyltransferase); Acyl-acyl carrier protein synthetase (Acyl-ACP synthetase)]. PLAYS A ROLE IN LYSOPHOSPHOLIPID ACYLATION. TRANSFERS FATTY ACIDS TO THE 1-POSITION VIA AN ENZYME-BOUND ACYL-ACP INTERMEDIATE IN THE PRESENCE OF ATP AND MG(2+). ITS PHYSIOLOGICAL FUNCTION IS TO REGENERATE PTDETN FROM 2-ACYL-GPE FORMED BY TRANSACYLATION REACTIONS OR DEGRADATION BY PHOSPHOLIPASE A1, TREMBL:Q8FEA6 (52% identity); SWISSPROT:P31119 (52% identity). Pfam (PF00501): AMP-binding enzyme. Pfam (PF01553): Acyltransferase. TIGRFAM (TIGR00530): 1-acyl-sn-glycerol-3-phosphate acyltransferases. SignalP reporting signal peptide.; High confidence in function and specificity; bifunctional Aas protein 171166 aas 4610086 aas Azoarcus sp. BH72 bifunctional Aas protein YP_931655.1 169028 R 62928 CDS YP_931656.1 119896443 4608243 complement(171247..171558) 1 NC_008702.1 Putative transcriptional repressor,; High confidence in function and specificity; putative transcriptional repressor 171558 smtB 4608243 smtB Azoarcus sp. BH72 putative transcriptional repressor YP_931656.1 171247 R 62928 CDS YP_931657.1 119896444 4609878 171735..172085 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BB4612 of Bordetella bronchiseptica of 37% (trembl|Q7WEM2(SRS)). No domains predicted. signal peptide 3 TMHs; Conserved hypothetical protein; hypothetical protein 172085 4609878 azo0152 Azoarcus sp. BH72 hypothetical protein YP_931657.1 171735 D 62928 CDS YP_931658.1 119896445 4606242 172088..172936 1 NC_008702.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0; F0F1 ATP synthase subunit A 172936 atpB 4606242 atpB Azoarcus sp. BH72 F0F1 ATP synthase subunit A YP_931658.1 172088 D 62928 CDS YP_931659.1 119896446 4608367 172992..173237 1 NC_008702.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0; F0F1 ATP synthase subunit C 173237 atpE 4608367 atpE Azoarcus sp. BH72 F0F1 ATP synthase subunit C YP_931659.1 172992 D 62928 CDS YP_931660.1 119896447 4608370 173279..173752 1 NC_008702.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.; F0F1 ATP synthase subunit B 173752 atpF 4608370 atpF Azoarcus sp. BH72 F0F1 ATP synthase subunit B YP_931660.1 173279 D 62928 CDS YP_931661.1 119896448 4608371 173756..174289 1 NC_008702.1 putive ATP synthase delta chain. Homology to atpH of E. coli of 32% (sprot|ATPD_ECOLI). Key component of the F-type ATPases (EC 3.6.3.14). The ATPase has 2 components,CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha, beta,gamma, delta, epsilon. CF(0) has three main subunits: a, b and c. Subunit delta seems to be part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) into CF(1) or is implicated in proton conduction. InterPro: ATP synthase delta (OSCP) subunit (IPR000711) Pfam: ATP synthase delta (OSCP) subunit no signal peptide no TMHs; High confidence in function and specificity; putative ATP synthase delta chain 174289 atpH 4608371 atpH Azoarcus sp. BH72 putative ATP synthase delta chain YP_931661.1 173756 D 62928 CDS YP_931662.1 119896449 4608373 174303..175841 1 NC_008702.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit; F0F1 ATP synthase subunit alpha 175841 atpA 4608373 atpA Azoarcus sp. BH72 F0F1 ATP synthase subunit alpha YP_931662.1 174303 D 62928 CDS YP_931663.1 119896450 4608366 175863..176732 1 NC_008702.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit; F0F1 ATP synthase subunit gamma 176732 atpG 4608366 atpG Azoarcus sp. BH72 F0F1 ATP synthase subunit gamma YP_931663.1 175863 D 62928 CDS YP_931664.1 119896451 4608372 176757..178157 1 NC_008702.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit; F0F1 ATP synthase subunit beta 178157 atpD 4608372 atpD Azoarcus sp. BH72 F0F1 ATP synthase subunit beta YP_931664.1 176757 D 62928 CDS YP_931665.1 119896452 4608369 178210..178635 1 NC_008702.1 part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane; F0F1 ATP synthase subunit epsilon 178635 atpC 4608369 atpC Azoarcus sp. BH72 F0F1 ATP synthase subunit epsilon YP_931665.1 178210 D 62928 CDS YP_931666.1 119896453 4608368 complement(178705..179283) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc0460 of R. solanacearum of 39% (trembl|Q8Y276(SRS) Pfam: DUF1243 This family consists of a number of hypothetical bacterial proteins of around 200 residues in length. The function of this family is unknown. no signal peptide no TMHs; hypothetical protein 179283 4608368 azo0161 Azoarcus sp. BH72 hypothetical protein YP_931666.1 178705 R 62928 CDS YP_931667.1 119896454 4606243 complement(179327..180064) 1 NC_008702.1 Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (EC 2.1.1.-). Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-14-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-14-benzoquinol (DMQH2) (By similarity). InterPro: ubiE/COQ5 methyltransferase hemK_rel_arch: methylase putative; High confidence in function and specificity; ubiquinone/menaquinone biosynthesis methyltransferase 180064 ubiE1 4606243 ubiE1 Azoarcus sp. BH72 ubiquinone/menaquinone biosynthesis methyltransferase YP_931667.1 179327 R 62928 CDS YP_931668.1 119896455 4610058 complement(180072..180494) 1 NC_008702.1 Conserved hypothetical protein. Homology to rs04443 of R. solanacearum of 69% (trembl|Q8Y279). Pfam: PF06155,Protein of unknown function (DUF971) Interpro: IPR010376; This family consists of several short bacterial proteins and one sequence (Q8RZ62) from Oryza sativa. The function of this family is unknown. No Signal Peptide. No TMH present.; hypothetical protein 180494 4610058 azo0163 Azoarcus sp. BH72 hypothetical protein YP_931668.1 180072 R 62928 CDS YP_931669.1 119896456 4606244 180644..181354 1 NC_008702.1 Phosphate regulon transcriptional regulatory protein,; High confidence in function and specificity; phosphate regulon transcriptional regulatory protein 181354 phoB 4606244 phoB Azoarcus sp. BH72 phosphate regulon transcriptional regulatory protein YP_931669.1 180644 D 62928 CDS YP_931670.1 119896457 4609550 181446..182717 1 NC_008702.1 Phosphate regulon sensor protein, 3 Signal P reporting signal peptide TMHMM reporting 1 transmembrane helices. MEMBER OF THE TWO-COMPONENT REGULATORY SYSTEM PHOR/PHOB INVOLVED IN THE PHOSPHATE REGULON GENES EXPRESSION. PHOR MAY FUNCTION AS A MEMBRANE-ASSOCIATED PROTEIN KINASE THAT PHOSPHORYLATES PHOB IN RESPONSE TO ENVIRONMENTAL SIGNALS.; High confidence in function and specificity; phosphate regulon sensor protein 182717 phoR 4609550 phoR Azoarcus sp. BH72 phosphate regulon sensor protein YP_931670.1 181446 D 62928 CDS YP_931671.1 119896458 4609551 complement(182683..184287) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3023 of Azoarcus sp. EbN1 of 61% (trembl:Q5P4D4). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 184287 4609551 azo0166 Azoarcus sp. BH72 hypothetical protein YP_931671.1 182683 R 62928 CDS YP_931672.1 119896459 4606245 complement(184317..184733) 1 NC_008702.1 55% HIT. Pfam:PF01230; HIT; 1. The Histidine Triad (HIT) motif, His-phi-His-phi-His-phi-phi (phi, a hydrophobic amino acid) was identified as being highly conserved in a variety of organisms.; hypothetical protein 184733 4606245 azo0167 Azoarcus sp. BH72 hypothetical protein YP_931672.1 184317 R 62928 CDS YP_931673.1 119896460 4606246 complement(184801..185625) 1 NC_008702.1 Conserved hypothetical glutamine amidotransferases class-II (Gn-AT), YafJ-type. Homology to ebA3026 of Azoarcus sp. EbN1 of 75% (gnl|keqq|eba:ebA3026(KEGG)). Pfam: Glutamine amidotransferases class-II. YafJ is a glutamine amidotransferase-like protein of unknown function found in prokaryotes, eukaryotes and archaea. YafJ has an Ntn hydrolase; Conserved hypothetical protein; glutamine amidotransferases class-II-like protein 185625 4606246 azo0168 Azoarcus sp. BH72 glutamine amidotransferases class-II-like protein YP_931673.1 184801 R 62928 CDS YP_931674.1 119896461 4606247 complement(185689..186207) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bll7463 of B. japonicum of 30% (tremble:Q89DH6). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 186207 4606247 azo0169 Azoarcus sp. BH72 hypothetical protein YP_931674.1 185689 R 62928 CDS YP_931675.1 119896462 4606248 complement(186300..187760) 1 NC_008702.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit B 187760 gatB 4606248 gatB Azoarcus sp. BH72 aspartyl/glutamyl-tRNA amidotransferase subunit B YP_931675.1 186300 R 62928 CDS YP_931676.1 119896463 4608862 complement(187818..188330) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to an orf of Dechloromonas aromatica of 41% (gi|46141061|ref|ZP_00152875.2|(NBCI ENTREZ)). No domains predicted. Signal P reporting signal peptide present. NO TMH present.; Conserved hypothetical protein; hypothetical protein 188330 4608862 azo0171 Azoarcus sp. BH72 hypothetical protein YP_931676.1 187818 R 62928 CDS YP_931677.1 119896464 4606249 complement(188336..189799) 1 NC_008702.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit A 189799 gatA 4606249 gatA Azoarcus sp. BH72 aspartyl/glutamyl-tRNA amidotransferase subunit A YP_931677.1 188336 R 62928 CDS YP_931678.1 119896465 4608861 complement(189852..190139) 1 NC_008702.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; some Mycoplasma proteins contain an N-terminal fusion to an unknown domain; aspartyl/glutamyl-tRNA amidotransferase subunit C 190139 gatC 4608861 gatC Azoarcus sp. BH72 aspartyl/glutamyl-tRNA amidotransferase subunit C YP_931678.1 189852 R 62928 CDS YP_931679.1 119896466 4608863 190256..191299 1 NC_008702.1 functions in MreBCD complex in some organisms; rod shape-determining protein MreB 191299 mreB 4608863 mreB Azoarcus sp. BH72 rod shape-determining protein MreB YP_931679.1 190256 D 62928 CDS YP_931680.1 119896467 4609272 191345..192262 1 NC_008702.1 in some organisms this protein is a transmembrane protein while in others it is periplasmic; involved in some organisms with other components of the MreBCD complex and with penicillin binding proteins in the periplasm or cell wall; rod shape-determining protein MreC 192262 mreC 4609272 mreC Azoarcus sp. BH72 rod shape-determining protein MreC YP_931680.1 191345 D 62928 CDS YP_931681.1 119896468 4609273 192266..192787 1 NC_008702.1 Rod shape-determining protein mreD. Involved in formation of the rod shape of the cell. May also contribute to regulation of formation of penicillin-binding proteins.; High confidence in function and specificity; rod shape-determining protein 192787 mreD 4609273 mreD Azoarcus sp. BH72 rod shape-determining protein YP_931681.1 192266 D 62928 CDS YP_931682.1 119896469 4609274 192797..194686 1 NC_008702.1 Penicillin-binding protein 2 (PBP-2). CELL WALL FORMATION; PBP2 IS RESPONSIBLE FOR THE DETERMINATION OF THE ROD SHAPE OF THE CELL. ITS SYNTHESIZE CROSS- LINKED PEPTIDOGLYCAN FROM THE LIPID INTERMEDIATES. InterPro: Penicillin binding protein transpeptidase domain; High confidence in function and specificity; penicillin-binding protein 194686 pbpA 4609274 pbpA Azoarcus sp. BH72 penicillin-binding protein YP_931682.1 192797 D 62928 CDS YP_931683.1 119896470 4609502 194683..195819 1 NC_008702.1 Rod shape-determining protein rodA. THIS IS ONE OF THE PROTEINS RESPONSIBLE FOR THE ROD SHAPE OF THE CELL. IT IS REQUIRED FOR THE EXPRESSION OF THE ENZYMATIC ACTIVITY OF PBP2 WHICH IS THOUGHT TO SYNTHESIZE PEPTIDOGLYCAN IN THE STEP OF INITIATION OF CELL ELONGATION.; High confidence in function and specificity; rod shape-determining protein 195819 rodA 4609502 rodA Azoarcus sp. BH72 rod shape-determining protein YP_931683.1 194683 D 62928 CDS YP_931684.1 119896471 4609756 195800..196840 1 NC_008702.1 RlpA-like protein precursor.; Function unclear; lipoprotein 196840 rlpA 4609756 rlpA Azoarcus sp. BH72 lipoprotein YP_931684.1 195800 D 62928 CDS YP_931685.1 119896472 4609729 196966..198096 1 NC_008702.1 Probable D-alanyl-D-alanine carboxypeptidase. Homology to dacD of E. coli of 41% (sprot|DACD_ECOLI) Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Pfam: D-alanyl-D-alanine carboxypeptidase signal peptide no TMHs; High confidence in function and specificity; D-alanyl-D-alanine carboxypeptidase 198096 dacC 4609729 dacC Azoarcus sp. BH72 D-alanyl-D-alanine carboxypeptidase YP_931685.1 196966 D 62928 CDS YP_931686.1 119896473 4608577 198136..198993 1 NC_008702.1 D-alanine aminotransferase (EC 2.6.1.21) (D-aspartate aminotransferase) (D-amino acid aminotransferase) (D-amino acid transaminase) (DAAT). Acts on the D-isomers of alanine Leucine aspartate glutamate aminobutyrate norvaline and asparagine (By similarity). InterPro: Aminotransferases class-IV; High confidence in function and specificity; D-alanine transaminase 198993 daaA 4608577 daaA Azoarcus sp. BH72 D-alanine transaminase YP_931686.1 198136 D 62928 CDS YP_931687.1 119896474 4608575 198986..199267 1 NC_008702.1 Conserved hypothetical protein. Homology to RSC0326 of Ralstonia solanacearum of 53% (tremble:Q8Y2K9) Has PF04359;Protein of unknown function (DUF493);This family includes several proteins of uncharacterised function. InterPro;IPR007454 No Signal Peptdie or TMH present.; hypothetical protein 199267 4608575 azo0182 Azoarcus sp. BH72 hypothetical protein YP_931687.1 198986 D 62928 CDS YP_931688.1 119896475 4606250 199264..199947 1 NC_008702.1 lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein; lipoate-protein ligase B 199947 lipB 4606250 lipB Azoarcus sp. BH72 lipoate-protein ligase B YP_931688.1 199264 D 62928 CDS YP_931689.1 119896476 4609147 199989..200936 1 NC_008702.1 catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group; lipoyl synthase 200936 lipA 4609147 lipA Azoarcus sp. BH72 lipoyl synthase YP_931689.1 199989 D 62928 CDS YP_931690.1 119896477 4609146 201005..201682 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP1518 of P. putida of 54% (trembl|Q88MQ2(SRS) No domains predicted Signal P reporting signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 201682 4609146 azo0185 Azoarcus sp. BH72 hypothetical protein YP_931690.1 201005 D 62928 CDS YP_931691.1 119896478 4606251 201756..203750 1 NC_008702.1 ATP-dependent DNA helicase rep (EC 3.6.1.-). REP HELICASE IS A SINGLE-STRANDED DNA-DEPENDENT ATPASE INVOLVED IN DNA REPLICATION; IT CAN INITIATE UNWINDING AT A NICK IN THE DNA. IT BINDS TO THE SINGLE-STRANDED DNA AND ACTS IN A PROGRESSIVE FASHION ALONG THE DNA IN THE 3 TO 5 DIRECTION (BY SIMILARITY). InterPro: UvrD/REP helicase.; High confidence in function and specificity; ATP-dependent DNA helicase 203750 rep 4606251 rep Azoarcus sp. BH72 ATP-dependent DNA helicase YP_931691.1 201756 D 62928 CDS YP_931692.1 119896479 4609713 complement(203837..204298) 1 NC_008702.1 Conserved hypothetical cytochrome c5. Homology to vc0168 of V. cloreae of 38% (tremble: Q9KVH8). This basic c-type monoheme cytochrome has an unusally low redox potential compaired with mitochaondrial cytochrome C. It is reactive with cytochrome c oxidase but not with reductase. Pfam: Cytrochrome C probable signal peptide no TMHs; Family membership; cytochrome c5 204298 4609713 azo0187 Azoarcus sp. BH72 cytochrome c5 YP_931692.1 203837 R 62928 CDS YP_931693.1 119896480 4608231 complement(210014..210739) 1 NC_008702.1 Putative benzil reductase. Homology to yueD of B. cereus of 32% (trembl|Q8RJ14). Belongs to the short-chain dehydrogenases/reductases (SDR) family. Transform benzil to (S)-benzion. Interpro: short-chain dehydrogenase/reductase SDR (IPR002198) Pfam: short chain dehydrogenase (PF00106) no signal peptide no TMHs; Family membership; short chain dehydrogenase 210739 yueD 4608231 yueD Azoarcus sp. BH72 short chain dehydrogenase YP_931693.1 210014 R 62928 CDS YP_931694.1 119896481 4610199 210838..211857 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of R. oxalatica of 31% (trembl|Q84ES1). Pfam: 37-kD nucleoid-associaed bacterial protein The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type Escherichia coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. no signal peptide no TMHs; hypothetical protein 211857 4610199 azo0190 Azoarcus sp. BH72 hypothetical protein YP_931694.1 210838 D 62928 CDS YP_931695.1 119896482 4606253 211933..212409 1 NC_008702.1 Entry name TREMBL:Q988L8 Prim. accession # Q988L8 InterPro IPR002539; MaoC_dehydratas. Identities = 63/131 (48%) Pfam PF01575; MaoC_dehydratas; 1. Number of predicted TMHs: 0 Probable MaoC protein (Phenylacetic acid degradation protein paaZ).; hypothetical protein 212409 4606253 azo0191 Azoarcus sp. BH72 hypothetical protein YP_931695.1 211933 D 62928 CDS YP_931696.1 119896483 4606254 212402..213313 1 NC_008702.1 Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid (By similarity). apbA_panE: 2-dehydropantoate 2-reductase; Function unclear; 2-dehydropantoate 2-reductase 213313 apbA1 4606254 apbA1 Azoarcus sp. BH72 2-dehydropantoate 2-reductase YP_931696.1 212402 D 62928 CDS YP_931697.1 119896484 4608325 complement(213319..213780) 1 NC_008702.1 Probable protein-disulfide reductase. Homology with trxC of E. coli of 45% (AAB88587). Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. InterPro: Thioredoxin Tigrfam: redox_disulf_1: redox-active disulfide Pfam: Thioredoxin no TMHs no signal peptide; Family membership; protein-disulfide reductase 213780 trxC1 4608325 trxC1 Azoarcus sp. BH72 protein-disulfide reductase YP_931697.1 213319 R 62928 CDS YP_931698.1 119896485 4610043 213935..214333 1 NC_008702.1 Putative trans-acting regulatory protein. Homology to hvrA of R. capsulatus of 27% (sprot|HVRA_RHOCA). The histone-like nucleoid-structuring (H-NS) protein belongs to a family of bacterial proteins that play a role in the formation of nucleoid structure and affect gene expression under certain conditions (e.g. light). Interpro: Histone -like nucleotide-structuring protein H-Ns (IPR001801) Pfam: H-NS histone family no signal peptide no TMHs; Family membership; putative trans-acting regulatory protein HvrA 214333 hvrA1 4610043 hvrA1 Azoarcus sp. BH72 putative trans-acting regulatory protein HvrA YP_931698.1 213935 D 62928 CDS YP_931699.1 119896486 4609055 214378..214626 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV3728 of Chromobacterium violaceum of 57% (tremble:Q7NRQ2) No domains predicted. No TMHs signal peptide present; Conserved hypothetical protein; hypothetical protein 214626 4609055 azo0195 Azoarcus sp. BH72 hypothetical protein YP_931699.1 214378 D 62928 CDS YP_931700.1 119896487 4606255 complement(214688..215722) 1 NC_008702.1 Conserved hypothetical transcription factor,; Conserved hypothetical protein; hypothetical protein 215722 4606255 azo0196 Azoarcus sp. BH72 hypothetical protein YP_931700.1 214688 R 62928 CDS YP_931701.1 119896488 4606256 215834..216118 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. Signal peptide. 1 TMHs; hypothetical protein 216118 4606256 azo0197 Azoarcus sp. BH72 hypothetical protein YP_931701.1 215834 D 62928 CDS YP_931702.1 119896489 4606257 complement(216106..217965) 1 NC_008702.1 Para-aminobenzoate synthase component I (EC 4.1.3.-) (ADC synthase). CATALYZES THE BIOSYNTHESIS OF 4-AMINO-4-DEOXYCHORISMATE (ADC) FROM CHORISMATE AND GLUTAMINE. InterPro: Anthranilate synthase component I and chorismate binding enzyme pabB: para-aminobenzoate synthase component I; Specificity unclear; para-aminobenzoate synthase component I 217965 pabB 4606257 pabB Azoarcus sp. BH72 para-aminobenzoate synthase component I YP_931702.1 216106 R 62928 CDS YP_931703.1 119896490 4609487 218051..219196 1 NC_008702.1 Exonuclease sbcD homolog. Probably involved in genetic recombination. InterPro: DNA repair exonuclease. sbcd: exonuclease SbcD.; High confidence in function and specificity; exonuclease SbcD 219196 sbcD 4609487 sbcD Azoarcus sp. BH72 exonuclease SbcD YP_931703.1 218051 D 62928 CDS YP_931704.1 119896491 4609840 219193..222264 1 NC_008702.1 Nuclease sbcCD subunit C. SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3->5 double strand exonuclease that can open hairpins. It also has a 5 single-strand endonuclease activity (By similarity). sbcc: exonuclease SbcC.; High confidence in function and specificity; exonuclease SbcC 222264 sbcC 4609840 sbcC Azoarcus sp. BH72 exonuclease SbcC YP_931704.1 219193 D 62928 CDS YP_931705.1 119896492 4609839 complement(222387..223202) 1 NC_008702.1 Arylesterase (EC 3.1.1.2) (Aryl-ester hydrolase) pir:C75401:39% identity, 52% similarity:hydrolase-related protein - Deinococcus radiodurans . IS A BIFUNCTIONAL ENZYME CAPABLE OF BOTH ESTER HYDROLYSIS AND HALOGENATION. ACTS ON MANY PHENOLIC ESTERS. Pfam: PF00561: alpha/beta hydrolase fold (74-271) InterPro: IPR000073: Alpha/beta hydrolase fold IPR000379: Esterase/lipase/thioesterase Pfam:Zn binding dehydrogenase mobB: molybdopterin-guanine dinucleotid; Family membership; aryl-ester hydrolase 223202 4609839 azo0201 Azoarcus sp. BH72 aryl-ester hydrolase YP_931705.1 222387 R 62928 CDS YP_931706.1 119896493 4606258 223457..224176 1 NC_008702.1 Conserved hypothetical protein. Homology only to r01975 of S. meliloti of 31% (trembl|Q92P21). no signal peptide no TMHs; hypothetical protein 224176 4606258 azo0202 Azoarcus sp. BH72 hypothetical protein YP_931706.1 223457 D 62928 CDS YP_931707.1 119896494 4606259 224493..225563 1 NC_008702.1 TREMBL:Q82XH5: 33% identity, 44% similarity InterPro:IPR007404; DUF457. Pfam: PF04307; DUF457 fnt: formate/nitrite transporter Non-secretory protein with signal peptide probability of 0.391 TMHMM predicted 4 transmembrane helices; Conserved hypothetical protein; hypothetical protein 225563 4606259 azo0203 Azoarcus sp. BH72 hypothetical protein YP_931707.1 224493 D 62928 CDS YP_931708.1 119896495 4606260 complement(225594..226691) 1 NC_008702.1 Description:abc transporter periplasmic binding protein.This is a family of basic membrane lipoproteins from Borrelia and various putative lipoproteins from other bacteria. All of these proteins are outer membrane proteins and are thus antigenic in nature when possessed by the pathogenic members of the family Probable CD4+ T cell-stimulating antigen precursor. TREMBL: Q8XXD8: 64% identity, 79% similarity InterPro:IPR003760; Bmp. Pfam: PF02608; Bmp Secretory protein with high signal peptide probablity (Signal P predicted). No. of transmembrane helices: 1 (TMHMM predicted) prok_nadp_idh: isocitrate dehydrogenas; Specificity unclear; lipoprotein 226691 bmpA 4606260 bmpA Azoarcus sp. BH72 lipoprotein YP_931708.1 225594 R 62928 CDS YP_931709.1 119896496 4608400 complement(226835..228079) 1 NC_008702.1 Hippurate hydrolase (EC 3.5.1.32) (Benzoylglycine amidohydrolase) (Hippuricase). TREMBL:Q7VUP2: 43% identity, 57% similarity InterPro; IPR002933; Peptidase_M20. InterPro; IPR010168; Pept_M20D_amidh. Pfam; PF01546; Peptidase_M20; 1. TIGRFAMs; TIGR01891; amidohydrolases TIGR00003: copper-ion-binding protein No signal peptide (Signal P predicted) No transmembrane helices present; High confidence in function and specificity; hippurate hydrolase 228079 hipO1 4608400 hipO1 Azoarcus sp. BH72 hippurate hydrolase YP_931709.1 226835 R 62928 CDS YP_931710.1 119896497 4608997 complement(228076..228660) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 228660 4608997 azo0206 Azoarcus sp. BH72 AraC family transcriptional regulator YP_931710.1 228076 R 62928 CDS YP_931711.1 119896498 4606261 228700..229056 1 NC_008702.1 Conserved hypothetical protein. Homology to bb1472 of B. bronchiseptica of 46% (trembl|Q7WMC0). Interpro: Protein of unknown function DUF861 (IPR008579). Pfam: Cupin_3 (former DUF861) (PFO5899). This family consists of several proteins which seem to be specific to plants and bacteria. The function of this family is unknown. No signal peptide. No TMHs; hypothetical protein 229056 4606261 azo0207 Azoarcus sp. BH72 hypothetical protein YP_931711.1 228700 D 62928 CDS YP_931712.1 119896499 4606262 complement(229064..229267) 1 NC_008702.1 Hypothetical protein, 45% identity to TrEMBL;Q7U8P5. Weak Homology with hits. No domains,repeats, motifs or features predicted Present.; Function unclear; hypothetical protein 229267 4606262 azo0208 Azoarcus sp. BH72 hypothetical protein YP_931712.1 229064 R 62928 CDS YP_931713.1 119896500 4606263 complement(229633..230862) 1 NC_008702.1 Sarcosine oxidase,subunit beta counts to the FAD dependent oxidoreductases. Similar to trembl|Q88AX8 (49%) and to trembl|Q987J9 (43%). Pfam (PF01266): FAD dependent oxidoreductase Pfam (PF01494): FAD binding domain Pfam (PF00070): Pyridine nucleotide-disulphide oxidoreductase; Function unclear; sarcosine oxidase subunit beta 230862 agaE 4606263 agaE Azoarcus sp. BH72 sarcosine oxidase subunit beta YP_931713.1 229633 R 62928 CDS YP_931714.1 119896501 4608285 complement(230859..232241) 1 NC_008702.1 Putative opine oxidase subunit A. Homology to ooxA of A. tumefaciens of 37% (sprot|OOXA_AGRT4). OXIDATIVE CLEAVAGE OF OCTOPINE INTO L-ARGININE AND PYRUVATE (BY SIMILARITY). InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327), NAD-bining site (IPR000205) Pfam: Pyridine nucleotide-disulphide oxidoreductase no signal peptide no TMHs; Family membership; putative opine oxidase subunit A 232241 ooxA 4608285 ooxA Azoarcus sp. BH72 putative opine oxidase subunit A YP_931714.1 230859 R 62928 CDS YP_931715.1 119896502 4609450 complement(232223..232552) 1 NC_008702.1 hypothetical protein 232552 4609450 azo0211 Azoarcus sp. BH72 hypothetical protein YP_931715.1 232223 R 62928 CDS YP_931716.1 119896503 4606264 complement(232564..233067) 1 NC_008702.1 Probable proline dehydrogenase transcriptional activator. Similar to sprot|PUTR_AGRTU (31% Agrobacterium tumefaciens, proline dehydrogenase transcriptional activator prp or putR) InterPro: IPR000485 HTH_AsnC_lrp. Pfam: PF01037 AsnC family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; proline dehydrogenase transcriptional activator 233067 prp 4606264 prp Azoarcus sp. BH72 proline dehydrogenase transcriptional activator YP_931716.1 232564 R 62928 CDS YP_931717.1 119896504 4609635 complement(233142..234524) 1 NC_008702.1 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) (78-diamino-pelargonic acid aminotransferase) (DAPA aminotransferase). bioA: adenosylmethionine--8-amino-7-oxononanoate aminotransferase; Specificity unclear; hypothetical protein 234524 4609635 azo0213 Azoarcus sp. BH72 hypothetical protein YP_931717.1 233142 R 62928 CDS YP_931718.1 119896505 4606265 complement(235059..235934) 1 NC_008702.1 Conserved hypothetical acetyltransferase. Homology to putative acetaltransferase (GNAT family) of P. syringae of 42% (trembl|Q88AY8). Pfam: Acetyltransferase (GNAT) family no signal peptide no TMHs; Family membership; acetyltransferase 235934 4606265 azo0214 Azoarcus sp. BH72 acetyltransferase YP_931718.1 235059 R 62928 CDS YP_931719.1 119896506 4606266 236153..237637 1 NC_008702.1 Putative aldehyde dehydrogenase (NAD+) (EC 1.2.1.3). Homology to adlH of E. coli of 35% (sprot|DHAL_ECOLI). Aldehyde dehydrogenases (EC: 1.2.1.3 and EC: 1.2.1.5) are enzymes which oxidize a wide variety of aliphatic and aromatic aldehydes using NADP as a cofactor. InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; High confidence in function and specificity; putative aldehyde dehydrogenase (NAD+) 237637 aldH 4606266 aldH Azoarcus sp. BH72 putative aldehyde dehydrogenase (NAD+) YP_931719.1 236153 D 62928 CDS YP_931720.1 119896507 4608295 237835..238500 1 NC_008702.1 Probable 2-haloalkanoic acid dehalogenase (EC 3.8.1.2) (L-2-haloacid dehalogenase) (Halocarboxylic acid halidohydrolase). TREMBL:Q92Y68: 68% identity, 82% similarity These proteins catalyze the hydrolytic dehalogenation of small L-2-haloalkanoic acids to yield the corresponding D-2-hydroxyalkanoic acids [1]. They belong to the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases InterPro:IPR006328; HAD_II. IPR006388; HAD_SF_IA_v2. IPR005834; Hydrolase. Pfam PF00702; Hydrolase rpiB_lacA_lacB: sugar-phosphate isomer No signal peptide No transmembrane helices; High confidence in function and specificity; haloalkanoic acid dehalogenase 238500 hadL 4608295 hadL Azoarcus sp. BH72 haloalkanoic acid dehalogenase YP_931720.1 237835 D 62928 CDS YP_931721.1 119896508 4608977 238541..239833 1 NC_008702.1 This family includes various FAD dependent oxidoreductases: Glycerol-3-phosphate dehydrogenase,Sarcosine oxidase beta subunit, D-alanine oxidase, D-aspartate oxidase. Similar to trembl|Q88AY5 (61%) and to sprot|ORDL_ECOLI (32%). Pfam (PF01266): D-amino acid oxidase ProSite (PS50205): NAD binding site; Family membership; FAD dependent oxidoreductase family protein 239833 ordL 4608977 ordL Azoarcus sp. BH72 FAD dependent oxidoreductase family protein YP_931721.1 238541 D 62928 CDS YP_931722.1 119896509 4609456 239850..241457 1 NC_008702.1 Part of the ABC transporter complex dppABCD involved in dipeptide import. Similar to the dipeptide periplasmic-binding protein dppA in E.coli. DIPEPTIDE-BINDING PROTEIN OF AN OSMOTIC-SHOCKABLE TRANSPORT SYSTEM. DPPA IS ALSO REQUIRED FOR PEPTIDE CHEMOTAXIS. InterPro: Bacterial extracellular solute-binding protein family 5 Signal peptide; Specificity unclear; putative dipeptide transport system periplasmic-binding protein 241457 dppA 4609456 dppA Azoarcus sp. BH72 putative dipeptide transport system periplasmic-binding protein YP_931722.1 239850 D 62928 CDS YP_931723.1 119896510 4608643 241462..242418 1 NC_008702.1 Part of the ABC transporter complex dppABCD involved in dipeptide import. Probably responsible for the translocation of the substrate across the membrane. Similar to the dipeptide permease, DppB in E.coli.; Specificity unclear; putative dipeptide transport system permease 242418 dppB 4608643 dppB Azoarcus sp. BH72 putative dipeptide transport system permease YP_931723.1 241462 D 62928 CDS YP_931724.1 119896511 4608644 242415..243290 1 NC_008702.1 Part of the ABC transporter complex dppABCD involved in dipeptide import. Probably responsible for the translocation of the substrate across the membrane. Similar to the dipeptide permease, DppC in E.coli.; Specificity unclear; ABC transporter permease 243290 dppC 4608644 dppC Azoarcus sp. BH72 ABC transporter permease YP_931724.1 242415 D 62928 CDS YP_931725.1 119896512 4608645 243283..245109 1 NC_008702.1 Part of the ABC transporter complex dppABCD involved in dipeptide import. Similar to the dipeptide transport membrane protein, DppD in E.coli Probably responsible for energy coupling to the transport system.; Specificity unclear; putative dipeptide transport system ATP-binding protein 245109 dppD 4608645 dppD Azoarcus sp. BH72 putative dipeptide transport system ATP-binding protein YP_931725.1 243283 D 62928 CDS YP_931726.1 119896513 4608646 245317..246789 1 NC_008702.1 Probable succinate-semialdehyde dehydrogenase [NAD(P)+]. Homology to gabD of R. eutropha of 68% (AAF19796). Catalysis of the reaction: succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H + H+. Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; High confidence in function and specificity; succinate semialdehyde dehydrogenase 246789 gabD1 4608646 gabD1 Azoarcus sp. BH72 succinate semialdehyde dehydrogenase YP_931726.1 245317 D 62928 CDS YP_931727.1 119896514 4608851 247001..247369 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to an orf of Polaromonas sp. JS666 of 34% (ZP_00360285) . No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 247369 4608851 azo0223 Azoarcus sp. BH72 hypothetical protein YP_931727.1 247001 D 62928 CDS YP_931728.1 119896515 4606267 247500..248717 1 NC_008702.1 Hypotethical outer membrane efflux protein. Homology to PA2525 of P. aeruginosa of 25%. signal peptide. no TMHs; outer membrane efflux protein 248717 4606267 azo0224 Azoarcus sp. BH72 outer membrane efflux protein YP_931728.1 247500 D 62928 CDS YP_931729.1 119896516 4606268 248714..249310 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank no domains predicted signal peptide no TMHs; hypothetical protein 249310 4606268 azo0225 Azoarcus sp. BH72 hypothetical protein YP_931729.1 248714 D 62928 CDS YP_931730.1 119896517 4606269 249307..250452 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to cc1787 of C. crescentus of 30% (trembl|Q9A7D6) no domains predicted signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 250452 4606269 azo0226 Azoarcus sp. BH72 hypothetical protein YP_931730.1 249307 D 62928 CDS YP_931731.1 119896518 4606270 250466..253597 1 NC_008702.1 Cobalt-zinc-cadmium resistance protein CzcA (cation efflux system protein CzcA). In A.eutrophus has a low cation transport activity for cobalt, it is essential for the expression of cobalt, zinc, and cadmium resistance.Czca and Czcb together would act in zinc efflux nearly as effectively as the complete czc efflux system (czcABC).Belongs to the acrb/acrd/acrf family. 41% similarity to the A.eutrophus CzcA protein. SWISSPROT:CZCA_ALCEU:P13511 InterPro: Heavy metal efflux pump CzcA. InterPro:IPR001036; Acrflvin_res.IPR004763: CzcA. Pfam:PF00873; ACR_tran; 1. InterPro:TIGR00914:Heavy metal efflux pump CzcA InterPro:PF00873:Acriflavin resistance protein Signal peptide: present.; High confidence in function and specificity; putative cation efflux system protein 253597 czcA1 4606270 czcA1 Azoarcus sp. BH72 putative cation efflux system protein YP_931731.1 250466 D 62928 CDS YP_931732.1 119896519 4608569 253594..254088 1 NC_008702.1 Hypothetical protein, 32% identity to TrEMBL;Q82T66 No Signal Peptide/Features/Domains/TMH detected.; hypothetical protein 254088 4608569 azo0228 Azoarcus sp. BH72 hypothetical protein YP_931732.1 253594 D 62928 CDS YP_931733.1 119896520 4606271 254346..256517 1 NC_008702.1 In E.coli this receptor binds the ferrichrome-iron ligand. It interacts with the tonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin. Signal peptide.; Specificity unclear; ferrichrome-iron TonB-dependent receptor 256517 fhuA1 4606271 fhuA1 Azoarcus sp. BH72 ferrichrome-iron TonB-dependent receptor YP_931733.1 254346 D 62928 CDS YP_931734.1 119896521 4608760 256540..257289 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RPA1846 of R.palustris of 39% (trembl:Q6N8Q7). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 257289 4608760 azo0230 Azoarcus sp. BH72 hypothetical protein YP_931734.1 256540 D 62928 CDS YP_931735.1 119896522 4606272 257937..259160 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to SO1157 of Shewanella oneidensis of 50% (trembl|Q8EHR0(SRS)). No domains predicted. Signal peptide. 7 TMHs; Conserved hypothetical protein; hypothetical protein 259160 4606272 azo0231 Azoarcus sp. BH72 hypothetical protein YP_931735.1 257937 D 62928 CDS YP_931736.1 119896523 4606273 259217..260059 1 NC_008702.1 Putative biopolymer transport protein ExbB. Homology to exbB of B. pertussis of 35%. ExbB is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. PFam: MotA/TolQ/ExbB proton channel family signals peptide most probable 4 TMHs; Family membership; putative biopolymer transport protein ExbB 260059 exbB1 4606273 exbB1 Azoarcus sp. BH72 putative biopolymer transport protein ExbB YP_931736.1 259217 D 62928 CDS YP_931737.1 119896524 4608696 260085..260513 1 NC_008702.1 putative biopolymer transport protein ExbD. ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton grandient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; Family membership; putative biopolymer transport protein ExbD 260513 exbD1 4608696 exbD1 Azoarcus sp. BH72 putative biopolymer transport protein ExbD YP_931737.1 260085 D 62928 CDS YP_931738.1 119896525 4608700 260535..260960 1 NC_008702.1 Putative biopolymer transport protein ExbD. Homology to exbD2 of X. campestris of 36% ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; Family membership; putative biopolymer transport protein ExbD 260960 exbD2 4608700 exbD2 Azoarcus sp. BH72 putative biopolymer transport protein ExbD YP_931738.1 260535 D 62928 CDS YP_931739.1 119896526 4608701 260989..261636 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to an orf of Rubrivivax gelatinosus of 55% (gi|47575633|ref|ZP_00245668.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 261636 4608701 azo0235 Azoarcus sp. BH72 hypothetical protein YP_931739.1 260989 D 62928 CDS YP_931740.1 119896527 4606274 complement(261880..262902) 1 NC_008702.1 Adenosine deaminase (Adenosine aminohydrolase). InterPro: Adenosine and AMP deaminase; High confidence in function and specificity; adenosine deaminase 262902 add 4606274 add Azoarcus sp. BH72 adenosine deaminase YP_931740.1 261880 R 62928 CDS YP_931741.1 119896528 4608278 complement(262974..263831) 1 NC_008702.1 Putative NAD-dependent deacetylase (EC 3.5.1.-) (Regulatory protein SIR2 homolog). Modulates the activities of several enzymes which are inactive in their acetylated form. Similar to SWISSPROT: sprot|NPD_AERPE (27% Aeropyrum pernix, NpdA or aq_2170) Pfam: PF02146 Sir2 family.; High confidence in function and specificity; putative NAD-dependent deacetylase 263831 npd1 4608278 npd1 Azoarcus sp. BH72 putative NAD-dependent deacetylase YP_931741.1 262974 R 62928 CDS YP_931742.1 119896529 4609408 complement(263828..264958) 1 NC_008702.1 Hypothetical membrane protein no homology to the data bank no domains predicted no signal predicted 7 TMHs; hypothetical protein 264958 4609408 azo0238 Azoarcus sp. BH72 hypothetical protein YP_931742.1 263828 R 62928 CDS YP_931743.1 119896530 4606275 complement(264970..266250) 1 NC_008702.1 Hypothetical protein, showing only very low similarity to known proteins (SWISSPROT: sprot|FRAH_ANASP; >10% Anabaena sp., FraH). Important Domains are: Pfam: PF00034 Cytochrome c. SMART: SM00438 ZnF_NFX TMHMM reporting 1 transmembrane helices.; hypothetical protein 266250 4606275 azo0239 Azoarcus sp. BH72 hypothetical protein YP_931743.1 264970 R 62928 CDS YP_931744.1 119896531 4606276 266442..267473 1 NC_008702.1 Putative ABC transporter periplasmic binding protein, DctP: TRAP dicarboxylate transporter.Binds C4-dicarboxylates; part of the binding-protein- dependent transport system for uptake of c4-dicarboxylates. 28% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide:present. TMhelix:1; Specificity unclear; putative C4-dicarboxylate-binding periplasmic protein 267473 dctP1 4606276 dctP1 Azoarcus sp. BH72 putative C4-dicarboxylate-binding periplasmic protein YP_931744.1 266442 D 62928 CDS YP_931745.1 119896532 4608602 267570..268058 1 NC_008702.1 C4-dicarboxylate transport system,permease small protein,DctQ. The Dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 22% DctQ. Pfam:PF04290; DctQ; 1. TMHelix:4.; High confidence in function and specificity; DctQ1 protein 268058 dctQ1 4608602 dctQ1 Azoarcus sp. BH72 DctQ1 protein YP_931745.1 267570 D 62928 CDS YP_931746.1 119896533 4608608 268055..269338 1 NC_008702.1 TRAP-type C4-dicarboxylate transport system, large permease component, DctM.The Dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 34% DctM.IPR000252; DedA.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1. Signal peptide: present. TMhelix:11.; Specificity unclear; TRAP-type C4-dicarboxylate transport system large permease 269338 dctM1 4608608 dctM1 Azoarcus sp. BH72 TRAP-type C4-dicarboxylate transport system large permease YP_931746.1 268055 D 62928 CDS YP_931747.1 119896534 4608597 complement(269367..270011) 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 270011 uidR 4608597 uidR Azoarcus sp. BH72 TetR family transcriptional regulator YP_931747.1 269367 R 62928 CDS YP_931748.1 119896535 4610067 270224..271426 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family, TREMBL:Q8XRL2 (55% identity); SWISSPROT:P31223 (53% identity). Pfam (PF00529): HlyD family secretion protein. SignalP predicting signal peptide. TC (8.A.1): The Membrane Fusion Protein (MFP) Family.; Family membership; membrane fusion protein 271426 4610067 azo0244 Azoarcus sp. BH72 membrane fusion protein YP_931748.1 270224 D 62928 CDS YP_931749.1 119896536 4606277 271438..274596 1 NC_008702.1 Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q8E8H2 (65% identity); SWISSPROT:P31224 (63% identity). InterPro (IPR001036): Acriflavin resistance protein. InterPro (IPR004764): Hydrophobe/amphiphile efflux-1 HAE1. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. Pfam (PF00873): AcrB/AcrD/AcrF family. TMHMM reporting 12 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 274596 4606277 azo0245 Azoarcus sp. BH72 RND efflux transporter permease YP_931749.1 271438 D 62928 CDS YP_931750.1 119896537 4606278 274611..276014 1 NC_008702.1 Probable outer membrane efflux protein. Homology to oprM of P. aeruginosa of 57%. Component of an efflux system that confers multidrug or multible antibiotic resistence. Pfam: Outer membrane efflux protein signal peptide no TMHS; Family membership; outer membrane efflux protein 276014 oprM1 4606278 oprM1 Azoarcus sp. BH72 outer membrane efflux protein YP_931750.1 274611 D 62928 CDS YP_931751.1 119896538 4609453 276172..276657 1 NC_008702.1 Bacterioferritin (BFR) (Cytochrome B-1) (Cytochrome B-557). In E. coli is an iron-storage protein consisting of 24 identical subunits that pack together to form a highly symmetrical, nearly spherical shell surrounding a central cavity of about 8 nm diameter.X-ray crystallographic studies have revealed a close structural similarity between BFR and the ferritins, a family of iron-storage proteins found in both eukaryota and prokaryota. Key role in iron homeostasis. InterPro: Bacterioferritin. Non-secretory protein; High confidence in function and specificity; putative bacterioferritin 276657 bfr1 4609453 bfr1 Azoarcus sp. BH72 putative bacterioferritin YP_931751.1 276172 D 62928 CDS YP_931752.1 119896539 4608386 276847..277365 1 NC_008702.1 Probable dnaK suppressor protein,; High confidence in function and specificity; dnaK suppressor protein 277365 dksA1 4608386 dksA1 Azoarcus sp. BH72 dnaK suppressor protein YP_931752.1 276847 D 62928 CDS YP_931753.1 119896540 4608624 277409..277567 1 NC_008702.1 Hypothetical protein. No homology to the data bank. No domains predicted. No signal peptide. No TMHS; hypothetical protein 277567 4608624 azo0249 Azoarcus sp. BH72 hypothetical protein YP_931753.1 277409 D 62928 CDS YP_931754.1 119896541 4606279 complement(277561..278289) 1 NC_008702.1 Hypothetical flavodoxin reductase. Homology to the N-terminal part of bb4317 of B. bronchisptica of 40% (trembl|Q7WFF6). InterPro: Oxidoreductase FAD and NAD(P)-binding domain ((IPR001433); NADH: cytochrome b5 reductase (CBR) (IPR001834) Pfam: Oxidorectase FAD-binding domain; Oxidoreductase NAD-binding domain no signal peptide no TMHs; flavodoxin reductase 278289 4606279 azo0250 Azoarcus sp. BH72 flavodoxin reductase YP_931754.1 277561 R 62928 CDS YP_931755.1 119896542 4606280 278417..279895 1 NC_008702.1 Conserved hypothetical membrane protein, 38% similarity to trEMBL;Q7WDT1,Putative membrane protein [BB4906] [Bordetella bronchiseptica (Alcaligenes bronchisepticus)]. TMHMM2 predicts 6 TMH's present. No signal peptide present.; Family membership; hypothetical protein 279895 4606280 azo0251 Azoarcus sp. BH72 hypothetical protein YP_931755.1 278417 D 62928 CDS YP_931756.1 119896543 4606281 279963..281318 1 NC_008702.1 conserved hypothetical protein, 54% identity to TrEMBL;Q9JYD1 Hypothetical protein NMB1644 [NMB1644] [Neisseria meningitidis(serogroup B)]. Has PF00009:Elongation factor Tu GTP binding domain:IPR000795 ProtSyn_GTPbind: This domain contains a P-loop motif, also found in several other families such as Ras, Arf and Myosin_head. Elongation factor Tu consists of three structural domains, this plus two C-terminal beta barrel domains. Elongation factors belong to a family of proteins that promote the GTP-dependent binding of aminoacyl tRNA to the A site of ribosomes during protein biosynthesis,and catalyse the translocation of the synthesised protein chain from the A to the P site. The proteins are all relatively similar in the vicinity of their C-termini, and are also highly; hypothetical protein 281318 4606281 azo0252 Azoarcus sp. BH72 hypothetical protein YP_931756.1 279963 D 62928 CDS YP_931757.1 119896544 4606282 complement(281341..282051) 1 NC_008702.1 Conserved hypothetical protein. Homology to MCA0921 of Methylococcus capsulatus of 54% (trembl:Q60AE3). N domains predicted. No TMHs. NO signal peptide.; hypothetical protein 282051 4606282 azo0253 Azoarcus sp. BH72 hypothetical protein YP_931757.1 281341 R 62928 CDS YP_931758.1 119896545 4606283 complement(282048..282746) 1 NC_008702.1 Conserved polysaccharide deacetylase. Homology to rs03397 of R. solanacearum of 62% (trembl|Q8Y2A8). Interpro: Polysaccharide deacetylase (IPR002509). Pfam: Polysaccharide deacetylase (PF01522). The family of polysaccharide deacetylases includes NodB (nodulation protein B from Rhizobium) which is a chitooligosaccharide deacetylase. It also includes chitin deacetylase from yeast, and endoxylanases which hydrolyses glucosidic bonds in xylan. No signal peptide. No TMHs; Conserved hypothetical protein; polysaccharide deacetylase 282746 4606283 azo0254 Azoarcus sp. BH72 polysaccharide deacetylase YP_931758.1 282048 R 62928 CDS YP_931759.1 119896546 4606284 complement(282948..283649) 1 NC_008702.1 Hypothetical protein. No good homology to the data bank. No domains predicted. No TMHs. No signal peptide.; hypothetical protein 283649 4606284 azo0255 Azoarcus sp. BH72 hypothetical protein YP_931759.1 282948 R 62928 CDS YP_931760.1 119896547 4606285 complement(283646..286057) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc0429 of R. solanacearum of 52% (trembl|Q8Y2A7). Tigrfam: 2A0604s01: protein-export membrane protein signal peptide 10 TMHs; Function unclear; hypothetical protein 286057 4606285 azo0256 Azoarcus sp. BH72 hypothetical protein YP_931760.1 283646 R 62928 CDS YP_931761.1 119896548 4606286 complement(286054..286632) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS03399 of R. solanacearum of 47% (trembl|Q8Y2A6(SRS)) No domains predicted. Signal Peptide present. No TMH reported.; Conserved hypothetical protein; hypothetical protein 286632 4606286 azo0257 Azoarcus sp. BH72 hypothetical protein YP_931761.1 286054 R 62928 CDS YP_931762.1 119896549 4606287 complement(286629..287561) 1 NC_008702.1 Conserved hypothetical acetyltransferase. Homology to rsc0431 of R. solanacearum of 61% (trembl|Q8Y2A5) Pfam: Bacterial lipid A biosynthesis acetyltransferase no signal peptide no TMHs; Conserved hypothetical protein; acetyltransferase 287561 4606287 azo0258 Azoarcus sp. BH72 acetyltransferase YP_931762.1 286629 R 62928 CDS YP_931763.1 119896550 4606288 complement(287558..287869) 1 NC_008702.1 Conserved hypothetical protein. Homology to xac 4098 of X. axonapodis of 36% (trembl|Q8PF87). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 287869 4606288 azo0259 Azoarcus sp. BH72 hypothetical protein YP_931763.1 287558 R 62928 CDS YP_931764.1 119896551 4606289 complement(287866..289215) 1 NC_008702.1 Entry name:- TREMBL:Q87EI7 InterPro:- IPR000873; AMP-bind. Pfam:- PF00501; AMP-binding; 2. Identity :- 45% Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; hypothetical protein 289215 4606289 azo0260 Azoarcus sp. BH72 hypothetical protein YP_931764.1 287866 R 62928 CDS YP_931765.1 119896552 4606290 complement(289232..289885) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc3402 of R. solanacearum of 46% (trembl|Q8Y2A3(SRS)) no domains predicted signal peptide 5 TMHs; Conserved hypothetical protein; hypothetical protein 289885 4606290 azo0261 Azoarcus sp. BH72 hypothetical protein YP_931765.1 289232 R 62928 CDS YP_931766.1 119896553 4606291 complement(289885..290169) 1 NC_008702.1 Similar to TREMBL:Q9PFA7 (61% identity); TREMBL:Q87AD9 (61% identity); SWISSPROT:Q7VKH6 (34% identity).; Function unclear; ORF1; hypothetical protein 290169 4606291 azo0262 Azoarcus sp. BH72 hypothetical protein YP_931766.1 289885 R 62928 CDS YP_931767.1 119896554 4606292 complement(290292..291683) 1 NC_008702.1 Probable sensor protein,; High confidence in function and specificity; two-component system histidine kinase 291683 4606292 azo0263 Azoarcus sp. BH72 two-component system histidine kinase YP_931767.1 290292 R 62928 CDS YP_931768.1 119896555 4606293 complement(291680..292336) 1 NC_008702.1 Transcriptional regulatory protein,; High confidence in function and specificity; transcriptional regulator 292336 qseB1 4606293 qseB1 Azoarcus sp. BH72 transcriptional regulator YP_931768.1 291680 R 62928 CDS YP_931769.1 119896556 4609686 292491..292763 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bd0091 of B. bacteriovorus of 52% (tremblnew|CAE77770(SRS)). no domains predicted. signal peptide. no TMHs.; Conserved hypothetical protein; hypothetical protein 292763 4609686 azo0265 Azoarcus sp. BH72 hypothetical protein YP_931769.1 292491 D 62928 CDS YP_931770.1 119896557 4606294 292777..293298 1 NC_008702.1 Conserved hypothetical cytochrome b561 family protein. Homology to rsc2354 of R. solanacearum of 43% (trembl|Q8XWW6). Involved in electron transfer from hydrogen to oxygene. Pfam: Nickel-dependen hydrogenase b-type cytochrome no signal peptide probable 4 TMHs; Family membership; cytochrome b561 family protein 293298 4606294 azo0266 Azoarcus sp. BH72 cytochrome b561 family protein YP_931770.1 292777 D 62928 CDS YP_931771.1 119896558 4606295 293415..294473 1 NC_008702.1 Putative membrane fusion protein. Homology to cnrb of A. eutrophus of 30% (pir|F47056). Proteins of the MFP family function as auxiliary proteins or 'adaptors',connecting a primary porter in the cytoplasmic membrane of a Gram-negative bacterium with an outer membrane factor protein that serves a porin or channel function in the outer membrane. In A. eutrophus is this protein involved in cobalt and nickel resistance. No domains predicted. No signal peptide No TMHs; Family membership; putative membrane fusion protein 294473 czcB 4606295 czcB Azoarcus sp. BH72 putative membrane fusion protein YP_931771.1 293415 D 62928 CDS YP_931772.1 119896559 4608571 294470..297574 1 NC_008702.1 Cobalt-zinc-cadmium resistance protein CzcA (cation efflux system protein CzcA). In A.eutrophus has a low cation transport activity for cobalt, it is essential for the expression of cobalt, zinc, and cadmium resistance.Czca and Czcb together would act in zinc efflux nearly as effectively as the complete czc efflux system (czcABC).Belongs to the acrb/acrd/acrf family. 41% similarity to the A.eutrophus CzcA protein. SWISSPROT:CZCA_ALCEU:P13511 InterPro: Heavy metal efflux pump CzcA. InterPro:IPR001036; Acrflvin_res.IPR004763: CzcA. Pfam:PF00873; ACR_tran; 1. InterPro:TIGR00914:Heavy metal efflux pump CzcA InterPro:PF00873:Acriflavin resistance protein Signal peptide: present.; High confidence in function and specificity; putative cation efflux system protein 297574 czcA2 4608571 czcA2 Azoarcus sp. BH72 putative cation efflux system protein YP_931772.1 294470 D 62928 CDS YP_931773.1 119896560 4608570 297571..297885 1 NC_008702.1 Conserved hypothetical protein.30% identical to TrEMBL; Q89MS9. Signal Peptide present.; hypothetical protein 297885 4608570 azo0269 Azoarcus sp. BH72 hypothetical protein YP_931773.1 297571 D 62928 CDS YP_931774.1 119896561 4606296 297885..299159 1 NC_008702.1 Hypothetical secreted protein. Homology to cnfc of Ralstonia sp CH34 of 28% (tremblnew|CAB82451(SRS). Pfam: outer membrane protein. Interpro: Type I antifreeze protein (IPR000104). singal peptide. no TMHs; hypothetical protein 299159 4606296 azo0270 Azoarcus sp. BH72 hypothetical protein YP_931774.1 297885 D 62928 CDS YP_931775.1 119896562 4606297 complement(299301..300887) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03000974 of Dechloromonas aromatica of 68% (gi|46140980|ref|ZP_00152750.2|(NBCI ENTREZ)). Pfam: Elongator protein 3, MiaB family, Radical SAM. This superfamily contains MoaA, NifB, PqqE, coproporphyrinogen III oxidase, biotin synthase and MiaB families, and includes a representative in the eukaryotic elongator subunit, Elp-3. Some members of the family are methyltransferases; Pfam: B12 binding domain. This domain binds to B12 (adenosylcobamide), it is found in several enzymes, such as glutamate mutase Q05488, methionine synthase Q99707 and methylmalonyl-CoA mutase. no signal peptide. no TMHs; hypothetical protein 300887 4606297 azo0271 Azoarcus sp. BH72 hypothetical protein YP_931775.1 299301 R 62928 CDS YP_931776.1 119896563 4606298 complement(301090..301368) 1 NC_008702.1 Hypothetical protein. No homology to protein of similar size in the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 301368 4606298 azo0272 Azoarcus sp. BH72 hypothetical protein YP_931776.1 301090 R 62928 CDS YP_931777.1 119896564 4606299 complement(301346..301972) 1 NC_008702.1 Putative RNA polymerase sigma factor, Myxococcus xanthus carQ; Alcaligenes eutrophus plasmid pMOL28-encoded cnrH; Escherichia coli fecI; Pseudomonas syringae hrpL; rpoE from Escherichia coli, Salmonella typhimurium and Haemophilus influenzae; Streptomyces coelicolor sigE; and Bacillus subtilis sigma factors sigV, sigX, sigY and sigZ.; High confidence in function and specificity; ECF family RNA polymerase sigma factor 301972 sigW 4606299 sigW Azoarcus sp. BH72 ECF family RNA polymerase sigma factor YP_931777.1 301346 R 62928 CDS YP_931778.1 119896565 4609868 complement(301969..302409) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to XCC0845 of X. campestris of 30% (trembl|Q8PC89). No domains predicted. Signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 302409 4609868 azo0274 Azoarcus sp. BH72 hypothetical protein YP_931778.1 301969 R 62928 CDS YP_931779.1 119896566 4606300 302583..302924 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CC3220 of Caulobacter crescentus of 34% (trembl|Q9A3I4(SRS)) No domains predicted. Signal Peptide present. NO TMH present.; Conserved hypothetical protein; hypothetical protein 302924 4606300 azo0275 Azoarcus sp. BH72 hypothetical protein YP_931779.1 302583 D 62928 CDS YP_931780.1 119896567 4606301 303050..303640 1 NC_008702.1 Putative Hypothetical protein. No Good homologous hits in the DB. No domains/features/signal peptide or motifs present.; hypothetical protein 303640 4606301 azo0276 Azoarcus sp. BH72 hypothetical protein YP_931780.1 303050 D 62928 CDS YP_931781.1 119896568 4606302 303727..304134 1 NC_008702.1 Hypothetical protein. Weak Homology. 39% identity to TrEMBL;Q8EWG7. No domains, repeats, motifs or features present.; hypothetical protein 304134 4606302 azo0277 Azoarcus sp. BH72 hypothetical protein YP_931781.1 303727 D 62928 CDS YP_931782.1 119896569 4606303 complement(304110..304865) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. Signal peptide. 1 TMHs; hypothetical protein 304865 4606303 azo0278 Azoarcus sp. BH72 hypothetical protein YP_931782.1 304110 R 62928 CDS YP_931783.1 119896570 4606304 305070..305825 1 NC_008702.1 Conserved hypothetical protein. Homology to BB1132 of B.bronchiseptica of 42% (tremble:Q7WNA8). No domains predicted. No Signal peptide or TMH present.; hypothetical protein 305825 4606304 azo0279 Azoarcus sp. BH72 hypothetical protein YP_931783.1 305070 D 62928 CDS YP_931784.1 119896571 4606305 305877..308081 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs03062 of R. solanacearum of 48% (tremble:Q8XQ05) no domains predicted signal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 308081 4606305 azo0280 Azoarcus sp. BH72 hypothetical protein YP_931784.1 305877 D 62928 CDS YP_931785.1 119896572 4606306 308084..308728 1 NC_008702.1 Outer membrane protein A precursor. Homology with hypothetical transmembrane protein and probable outer membrane protein of 56% as well as with motB (flagellar motor protein B) from amino acid 84 of 34%. Pfam: OmpA no signal peptide probable 1 TMH; Conserved hypothetical protein; hypothetical protein 308728 4606306 azo0281 Azoarcus sp. BH72 hypothetical protein YP_931785.1 308084 D 62928 CDS YP_931786.1 119896573 4606307 308721..309476 1 NC_008702.1 Conserved hypothetical protein. Homology to RS03060 of R.solanacearum of 35% (tremble:Q8XQ03) No domains predicted. No signal peptide or TMH present.; hypothetical protein 309476 4606307 azo0282 Azoarcus sp. BH72 hypothetical protein YP_931786.1 308721 D 62928 CDS YP_931787.1 119896574 4606308 complement(309473..310132) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no TMHs. no signal peptide; hypothetical protein 310132 4606308 azo0283 Azoarcus sp. BH72 hypothetical protein YP_931787.1 309473 R 62928 CDS YP_931788.1 119896575 4606309 complement(310129..311193) 1 NC_008702.1 Pfam (PF02801): Beta-ketoacyl synthase, C-terminal domain. Pfam (PF00109): Beta-ketoacyl synthase, N-terminal domain.; Function unclear; putative Beta-ketoacyl synthase family protein 311193 4606309 azo0284 Azoarcus sp. BH72 putative Beta-ketoacyl synthase family protein YP_931788.1 310129 R 62928 CDS YP_931789.1 119896576 4606310 complement(311190..311462) 1 NC_008702.1 Conserved hypothetical acyl carrier protein. Homology to psptO5093 of P. syringae of 44% (trembl|Q87V48). Interpro: Phosphopanteteine-binding (IPR00613). Pfam: Phosphopantetheine attachment site (PF00550) Phosphopantetheine (or pantetheine 4' phosphate) is the prosthetic group of acyl carrier proteins (ACP) in some multienzyme complexes where it serves as a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. Tigrfam: acyl_carrier: acyl carrier protein. no signal peptide. no TMHs; Conserved hypothetical protein; acyl carrier protein 311462 4606310 azo0285 Azoarcus sp. BH72 acyl carrier protein YP_931789.1 311190 R 62928 CDS YP_931790.1 119896577 4606311 complement(311480..312718) 1 NC_008702.1 Similar to TREMBL:Q7NFQ1 (27% identity); TREMBL:Q97E48 (20% identity); TREMBL:Q8FWI9 (21% identity). TMHMM predicting six transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; putative ABC transporter permease 312718 4606311 azo0286 Azoarcus sp. BH72 putative ABC transporter permease YP_931790.1 311480 R 62928 CDS YP_931791.1 119896578 4606312 complement(312733..313155) 1 NC_008702.1 Probable DNA mismatch repair protein mutS. TREMBL:Q92SQ4:42% identity, 55% similarity. InterPro:IPR002716; PIN. Pfam: PF01850; PIN Absence of signal peptide Absence of transmembrane helices L17: ribosomal protein L17; Function unclear; hypothetical protein 313155 4606312 azo0287 Azoarcus sp. BH72 hypothetical protein YP_931791.1 312733 R 62928 CDS YP_931792.1 119896579 4606313 complement(313152..313379) 1 NC_008702.1 no significant homologies; hypothetical protein 313379 4606313 azo0288 Azoarcus sp. BH72 hypothetical protein YP_931792.1 313152 R 62928 CDS YP_931793.1 119896580 4606314 complement(313482..314405) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q8R788 (35% identity); TREMBL:Q81S77 (37% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR003593): AAA ATPase. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; ABC transporter ATP-binding protein 314405 4606314 azo0289 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931793.1 313482 R 62928 CDS YP_931794.1 119896581 4606315 complement(314413..315417) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001929 of Dechloromonas aromatica of 68% (gi|53730629|ref|ZP_00348881.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 315417 4606315 azo0290 Azoarcus sp. BH72 hypothetical protein YP_931794.1 314413 R 62928 CDS YP_931795.1 119896582 4606316 complement(315418..315813) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001928 of Dechloromonas aromatica of 59% (gi|41724574|ref|ZP_00151411.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 315813 4606316 azo0291 Azoarcus sp. BH72 hypothetical protein YP_931795.1 315418 R 62928 CDS YP_931796.1 119896583 4606317 complement(315826..316980) 1 NC_008702.1 FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; this condensing enzyme differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs; 3-oxoacyl-ACP synthase 316980 darB 4606317 darB Azoarcus sp. BH72 3-oxoacyl-ACP synthase YP_931796.1 315826 R 62928 CDS YP_931797.1 119896584 4608587 complement(316973..317914) 1 NC_008702.1 Probable dialkylrecorsinol condensing enzyme. Homology to darA of P. aurantiaca of 56% (trembl|Q84H30(SRS)) no domains predicted no signal peptide 1 TMHs; Family membership; dialkylrecorsinol condensing protein 317914 darA 4608587 darA Azoarcus sp. BH72 dialkylrecorsinol condensing protein YP_931797.1 316973 R 62928 CDS YP_931798.1 119896585 4608586 complement(317928..319742) 1 NC_008702.1 INVOLVED IN CELL WALL BIOSYNTHESIS AND MAY ALSO ACT AS A SENSOR OF EXTERNAL PENICILLINS, TREMBL:Q7UMP8 (30% identity); TREMBL:Q988N4 (27% identity).; Family membership; putative penicillin-binding protein 319742 4608586 azo0294 Azoarcus sp. BH72 putative penicillin-binding protein YP_931798.1 317928 R 62928 CDS YP_931799.1 119896586 4606318 320022..320456 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2374 of C.violaceum of 31% (tremble:Q7NVG9). No domains predicted. No signal peptide or TMH present.; hypothetical protein 320456 4606318 azo0295 Azoarcus sp. BH72 hypothetical protein YP_931799.1 320022 D 62928 CDS YP_931800.1 119896587 4606319 complement(320512..321492) 1 NC_008702.1 InterPro:IPR001279- Metallo-beta-lactamase superfamily,glyoxalase II family, that catalyse the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid. TREMBL:Q7WEK1-55% Identity. Pfam-Presence of phosphoribosyltransferase, tissue factor, and GMP reductaseC like domains Signal P predicting signal peptide and TMHMM predicting transmembrane helices. ccoP: cytochrome c oxidase cbb3-type s; High confidence in function and specificity; metallo-beta-lactamase superfamily protein 321492 4606319 azo0296 Azoarcus sp. BH72 metallo-beta-lactamase superfamily protein YP_931800.1 320512 R 62928 CDS YP_931801.1 119896588 4606320 complement(321614..321958) 1 NC_008702.1 Conserved hypothetical protein, 66% similarity to TrEMBL; Q6NA86. Signal peptide present. No TMH reported present.; hypothetical protein 321958 4606320 azo0297 Azoarcus sp. BH72 hypothetical protein YP_931801.1 321614 R 62928 CDS YP_931802.1 119896589 4606321 complement(321964..322572) 1 NC_008702.1 pathway:carnitine metabolism (conversion of carnitine to gamma-butyrobetaine). TREMBL:Q9F9V3: 71% identity, 83% similarity similarity:belongs to the transferase hexapeptide repeat family Carnitine operon protein caiE. InterPro:IPR001451; Hexapep_transf. Pfam:PF00132; Hexapep InterPro: Bacterial transferase hexapeptide repeat TIGR00094: conserved hypothetical protein; High confidence in function and specificity; phenyl acetic acid degradation protein 322572 paaY 4606321 paaY Azoarcus sp. BH72 phenyl acetic acid degradation protein YP_931802.1 321964 R 62928 CDS YP_931803.1 119896590 4609485 complement(322766..324430) 1 NC_008702.1 Conserved hypothetical dehydrogenase. Homology to paaZ of A. evansii of 83% (trembl|Q9F9V2). Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; Family membership; dehydrogenase 324430 paaZ 4609485 paaZ Azoarcus sp. BH72 dehydrogenase YP_931803.1 322766 R 62928 CDS YP_931804.1 119896591 4609486 324719..325522 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 325522 paaG1 4609486 paaG1 Azoarcus sp. BH72 enoyl-CoA hydratase YP_931804.1 324719 D 62928 CDS YP_931805.1 119896592 4609472 325526..327073 1 NC_008702.1 converts 3-hydroxyadipyl-CoA to beta-ketoadipyl-CoA in phenylacetate degradation; 3-hydroxyacyl-CoA dehydrogenase 327073 paaH1 4609472 paaH1 Azoarcus sp. BH72 3-hydroxyacyl-CoA dehydrogenase YP_931805.1 325526 D 62928 CDS YP_931806.1 119896593 4609478 327066..327536 1 NC_008702.1 Phenylacetic acid aerobic catabolism. Similar to TREMBL:Q9F9V0 (74% identity); SWISSPROT:P76084 (48% identity). InterPro (IPR003736): Phenylacetic acid degradation-related protein. Pfam (DUF157): Uncharacterized protein PaaI, COG2050; High confidence in function and specificity; phenylacetic acid degradation protein PaaI 327536 paaI 4609478 paaI Azoarcus sp. BH72 phenylacetic acid degradation protein PaaI YP_931806.1 327066 D 62928 CDS YP_931807.1 119896594 4609480 327612..328934 1 NC_008702.1 Phenylacetate-coenzyme A ligase (EC 6.2.1.30) (Phenylacetyl-CoA ligase) (PA-CoA ligase). catalyzes the activation of phenylacetic acid to phenylacetyl-coA; High confidence in function and specificity; phenylacetate--CoA ligase 328934 paaK 4609480 paaK Azoarcus sp. BH72 phenylacetate--CoA ligase YP_931807.1 327612 D 62928 CDS YP_931808.1 119896595 4609483 328992..329987 1 NC_008702.1 with PaaBCDE catalyzes the hydroxylation of phenylacetyl-CoA; phenylacetate-CoA oxygenase subunit PaaA 329987 paaA 4609483 paaA Azoarcus sp. BH72 phenylacetate-CoA oxygenase subunit PaaA YP_931808.1 328992 D 62928 CDS YP_931809.1 119896596 4609465 330089..330376 1 NC_008702.1 with PaaBCDE catalyzes the hydroxylation of phenylacetyl-CoA; involved in phenylacetate degradation; phenylacetate-CoA oxygenase subunit PaaB 330376 paaB 4609465 paaB Azoarcus sp. BH72 phenylacetate-CoA oxygenase subunit PaaB YP_931809.1 330089 D 62928 CDS YP_931810.1 119896597 4609466 330388..331158 1 NC_008702.1 Probable phenylacetic acid degradation protein PaaC. Homology to paaI of P. putida of 46% (trembl|O84983). Part of a catabolic pathway of phenylacetic acid. These proteins forms part of a dioxygenase complex. Interpro: Phenylacetic acid catabolic (IPR007814) Pfam: Phenylacetic acid catabolic protein (PF05138) no signal peptide no TMHs; High confidence in function and specificity; phenylacetic acid degradation protein PaaC 331158 paaC 4609466 paaC Azoarcus sp. BH72 phenylacetic acid degradation protein PaaC YP_931810.1 330388 D 62928 CDS YP_931811.1 119896598 4609467 331218..331709 1 NC_008702.1 Probable pheylacetic acid degradation protein PaaD. Homology to phaH of P. putida of 54% (trembl|Q88HS8) Interpro: Protein of unknown function DUF59 (IPR002744) Pfam: Domain of unknoen function DUF59 (PF01883). Domain of unknown function DUF59;IPR002744; This family includes prokaryotic proteins of unknown function. The family also includes PhaH O84984 from Pseudomonas putida. PhaH forms a complex with PhaF O84982, PhaG O84983 and PhaI O84985,which hydroxylates phenylacetic acid to 2-hydroxyphenylacetic acid. So members of this family may all be components of ring hydroxylating complexes. no signal peptide no TMHs; High confidence in function and specificity; phenylacetic acid degradation protein PaaD 331709 paaD 4609467 paaD Azoarcus sp. BH72 phenylacetic acid degradation protein PaaD YP_931811.1 331218 D 62928 CDS YP_931812.1 119896599 4609468 331742..332809 1 NC_008702.1 Probable phenylacetic acid degradation NADH oxidoreductase paaE. Homology to paaE of E. coli of 50% (sprot|PAAE_ECOLI). Probable PART OF A MULTICOMPONENT OXYGENASE involved in aerobic degradation of phenylacetic acid. InterPro: Oxidoreductase FAD and NAD(P)-binding domain(IPR001433); 2Fe-2S Ferredoxin (IPR006057); Ferredoxin (IPR001041); NADH: cytochrome b5 reductase (CBR) (IPR001834) Pfam: Oxidoreductase FAD-binding domain; Oxidoreductase NAD-binding domain; 2Fe-2S iorn-sulfur cluster binding domain no signal peptide no TMHs; High confidence in function and specificity; phenylacetic acid degradation NADH oxidoreductase PaaE 332809 paaE 4609468 paaE Azoarcus sp. BH72 phenylacetic acid degradation NADH oxidoreductase PaaE YP_931812.1 331742 D 62928 CDS YP_931813.1 119896600 4609469 332869..333513 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 333513 paaR 4609469 paaR Azoarcus sp. BH72 TetR family transcriptional regulator YP_931813.1 332869 D 62928 CDS YP_931814.1 119896601 4609484 333560..334762 1 NC_008702.1 catalyzes the thiolytic cleavage of beta-ketoadipyl-CoA to succinate and acetyl-CoA; beta-ketoadipyl CoA thiolase 334762 paaJ1 4609484 paaJ1 Azoarcus sp. BH72 beta-ketoadipyl CoA thiolase YP_931814.1 333560 D 62928 CDS YP_931815.1 119896602 4609481 334888..336033 1 NC_008702.1 ABC transporter substrate binding proteins count to the family of extracellular ligand binding proteins. It is a component of the high affinity amino acid transport system. Similar to trembl|Q8XU64 (71%) and to sprot|LIVJ_ECOLI (16%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; ABC transporter substrate-binding protein 336033 4609481 azo0311 Azoarcus sp. BH72 ABC transporter substrate-binding protein YP_931815.1 334888 D 62928 CDS YP_931816.1 119896603 4606322 336157..337194 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q7W5E8 (62%), to sprot|BRAD_PSEAE (25%) and to sprot|LIVH_ECOLI (26%). Pfam (PF02653): Branched-chain amino acid transport system / permease component TMHMM reporting nine Tmhelix.; Specificity unclear; ABC transporter permease 337194 4606322 azo0312 Azoarcus sp. BH72 ABC transporter permease YP_931816.1 336157 D 62928 CDS YP_931817.1 119896604 4606323 337260..339053 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. The integral inner-membrane proteins translocate the substrate across the membrane. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XRX5 (54%) and to trembl|Q8UFI6 (47%). Pfam: ABC transporter Pfam (PF02653): Branched-chain amino acid transport system / permease component Smart : AAA ATPase SignalP reporting Signal peptide. TMHMM reporting nine Tmhelix.; Specificity unclear; ABC transporter ATP-binding protein/permease 339053 4606323 azo0313 Azoarcus sp. BH72 ABC transporter ATP-binding protein/permease YP_931817.1 337260 D 62928 CDS YP_931818.1 119896605 4606324 339053..339814 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XRX4 (68%) and to trembl|Q7WCY5 (63%). Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 339814 4606324 azo0314 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931818.1 339053 D 62928 CDS YP_931819.1 119896606 4606325 340402..340677 1 NC_008702.1 DNA-binding protein HU (DNA-binding protein II) (HB). Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it and thus to prevent its denaturation under extreme environmental conditions. InterPro: Bacterial histone-like DNA-binding protein; High confidence in function and specificity; DNA-binding protein HU 340677 hupB 4606325 hupB Azoarcus sp. BH72 DNA-binding protein HU YP_931819.1 340402 D 62928 CDS YP_931820.1 119896607 4609042 341121..342464 1 NC_008702.1 ATP-dependent RNA helicase; Family membership; putative ATP-dependent RNA helicase 342464 rhlE1 4609042 rhlE1 Azoarcus sp. BH72 putative ATP-dependent RNA helicase YP_931820.1 341121 D 62928 CDS YP_931821.1 119896608 4609716 complement(342549..343016) 1 NC_008702.1 Regulates rRNA biosynthesis by transcriptional antitermination; transcription antitermination protein NusB 343016 nusB 4609716 nusB Azoarcus sp. BH72 transcription antitermination protein NusB YP_931821.1 342549 R 62928 CDS YP_931822.1 119896609 4609434 complement(343013..343483) 1 NC_008702.1 RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; beta subunit of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not; 6,7-dimethyl-8-ribityllumazine synthase 343483 ribH 4609434 ribH Azoarcus sp. BH72 6,7-dimethyl-8-ribityllumazine synthase YP_931822.1 343013 R 62928 CDS YP_931823.1 119896610 4609725 complement(343535..344626) 1 NC_008702.1 bifunctional enzyme DHBP synthase/GTP cyclohydrolase II-like protein; functions in riboflavin synthesis; bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II-like protein 344626 ribAB 4609725 ribAB Azoarcus sp. BH72 bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II-like protein YP_931823.1 343535 R 62928 CDS YP_931824.1 119896611 4609721 complement(344623..345237) 1 NC_008702.1 catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine; riboflavin synthase subunit alpha 345237 ribE 4609721 ribE Azoarcus sp. BH72 riboflavin synthase subunit alpha YP_931824.1 344623 R 62928 CDS YP_931825.1 119896612 4609723 complement(345296..347116) 1 NC_008702.1 Putative Protein disulfide-isomerase (EC 5.3.4.1). Homology to dsbD of E. coli of 37% (sprot|DSBD_ECOLI). Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps (By similarity). Pfam: Cytochrom C biogenesis protein transporter signal peptide probable 8 TMHs; Family membership; putative protein disulfide-isomerase 347116 cutA2 4609723 cutA2 Azoarcus sp. BH72 putative protein disulfide-isomerase YP_931825.1 345296 R 62928 CDS YP_931826.1 119896613 4608545 complement(347169..347492) 1 NC_008702.1 Periplasmic divalent cation tolerance protein .36% similarity to E.coli CutA1 protein involved in copper tolerance. InterPro:IPR004323; CutA1. Pfam:PF03091; CutA1; 1.; High confidence in function and specificity; divalent cation tolerance protein 347492 cutA1 4608545 cutA1 Azoarcus sp. BH72 divalent cation tolerance protein YP_931826.1 347169 R 62928 CDS YP_931827.1 119896614 4608544 complement(347536..348780) 1 NC_008702.1 TREMBL:Q7MBF3: 80% identity, 89% similarity InterPro:IPR004136; 2nprop_dioxygen. IPR003009; FMN_enzyme. Pfam: PF03060; NPD tim: triosephosphate isomerase Non-secretory protein with no signal peptide. TMHMM predicted no transmembrane helices.; High confidence in function and specificity; 2-nitropropane dioxygenase 348780 naoA 4608544 naoA Azoarcus sp. BH72 2-nitropropane dioxygenase YP_931827.1 347536 R 62928 CDS YP_931828.1 119896615 4609327 348990..349301 1 NC_008702.1 Conserved hypothetical protein. Homology to GSU0176 of G.sulfurreducens of 42% (tremble:Q74GR9) No domains predicted. No signal peptide or TMH reported present.; hypothetical protein 349301 4609327 azo0324 Azoarcus sp. BH72 hypothetical protein YP_931828.1 348990 D 62928 CDS YP_931829.1 119896616 4606326 349421..350749 1 NC_008702.1 Putative flavocytochrome protein. 48% FAD_binding_6.IPR001433; Oxred_FAD/NAD(P). Pfam:PF00970; FAD_binding_6; 1.PF00175; NAD_binding_1; 1. TMhelix: 6. Siganl peptide: present.; Function unclear; putative flavocytochrome protein 350749 4606326 azo0325 Azoarcus sp. BH72 putative flavocytochrome protein YP_931829.1 349421 D 62928 CDS YP_931830.1 119896617 4606327 350997..351800 1 NC_008702.1 conserved hypothetical protein. Homology to cv0811 of C. violaceum of 43% (trembl|Q7NPT0(SRS) no domains predicted no signal peptide no TMHs; hypothetical protein 351800 4606327 azo0326 Azoarcus sp. BH72 hypothetical protein YP_931830.1 350997 D 62928 CDS YP_931831.1 119896618 4606328 complement(351835..352428) 1 NC_008702.1 Putative TetR family transcriptional regulator,; Conserved hypothetical protein; TetR family transcriptional regulator 352428 4606328 azo0327 Azoarcus sp. BH72 TetR family transcriptional regulator YP_931831.1 351835 R 62928 CDS YP_931832.1 119896619 4606329 352603..353745 1 NC_008702.1 In enteric bacteria such as E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar to sprot|LIVK_SALTY (23%) and to trembl|Q8XUX2 (46%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative leucine-specific binding protein 353745 livK1 4606329 livK1 Azoarcus sp. BH72 putative leucine-specific binding protein YP_931832.1 352603 D 62928 CDS YP_931833.1 119896620 4609153 complement(353800..354273) 1 NC_008702.1 Conserved hypothetical protein. Homology to SCO5300 of Streptomyces coelicolor of 46% (tremble:Q9XAE9). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 354273 4609153 azo0329 Azoarcus sp. BH72 hypothetical protein YP_931833.1 353800 R 62928 CDS YP_931834.1 119896621 4606330 complement(354344..355339) 1 NC_008702.1 HTH-type transcriptional regulator blaA (Beta-lactamase regulatory protein blaA). Positive regulator of the expression of the gene (blab) for beta-lactamase. Similar to SWISSPROT: sprot|BLAA_PROVU (32% Proteus vulgaris, HTH-type transcriptional regulator BlaA) InterPro: IPR000847 HTH_LysR. IPR009058 Winged helix DNA-binding. Pfam: PF00126 Bacterial regulatory helix-turn-helix protein, lysR family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; LysR family transcriptional regulator 355339 blaA 4606330 blaA Azoarcus sp. BH72 LysR family transcriptional regulator YP_931834.1 354344 R 62928 CDS YP_931835.1 119896622 4608398 355556..356023 1 NC_008702.1 Conserved hypothetical protein, 41% similarity to TrEMBL;Q88JC7. Has IPR005544 DUF_HHE:PF03794;Domain of Unknown function:This domain normally occurs as tandem repeats and is found in bacteria, yeast and plants. It contains two fully conserved histidines and one glutamate residue. Members of the family include DnrN, NorA and ScdA, which have been implicated in NO response and cell wall physiology. Has no Signal peptide or TMH reported present.; hypothetical protein 356023 4608398 azo0331 Azoarcus sp. BH72 hypothetical protein YP_931835.1 355556 D 62928 CDS YP_931836.1 119896623 4606331 complement(356048..357376) 1 NC_008702.1 Hypothetical protein yqhO. This family consists of various patatin glycoproteins from the total soluble protein in potato tubers.Patatin is a storage protein but it also has the enzymatic activity of lipid acyl hydrolase, catalysing the cleavage of fatty acids from membrane lipids SPROT:P54513: 23% identity, 33% similarity SubtiList: BG11703; yqhO. InterPro:IPR002641; Patatin. Pfam:PF01734; Patatin No signal peptide TMHMM predicted 2 transmembrane helices mobB: molybdopterin-guanine dinucleot; Family membership; putative esterase 357376 4606331 azo0332 Azoarcus sp. BH72 putative esterase YP_931836.1 356048 R 62928 CDS YP_931837.1 119896624 4606332 357462..358568 1 NC_008702.1 Hypothetical protein Rv1063c/MT1093/Mb1092c. TREMBL:Q89EP5: 29% identity, 42% similarity InterPro:IPR002641; Patatin. Pfam:PF01734; Patatin 2A0108: nitrate transporter Absence of signal peptide No. of TMH's: 2 (TMHMM predicted).; Family membership; putative esterase 358568 4606332 azo0333 Azoarcus sp. BH72 putative esterase YP_931837.1 357462 D 62928 CDS YP_931838.1 119896625 4606333 complement(358579..359157) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BPP2140 of Bordetella parapertussis 0f 44% (trembl|Q7W8J3(SRS)) No domains predicted. Signal peptide present. No TMH present. 6PTHBS: 6-pyruvoyl tetrahydrobiopterin; Conserved hypothetical protein; hypothetical protein 359157 4606333 azo0334 Azoarcus sp. BH72 hypothetical protein YP_931838.1 358579 R 62928 CDS YP_931839.1 119896626 4606334 359461..360240 1 NC_008702.1 EAL-domain containing protein, ):This domain is found in diverse bacterial signaling proteins. It is called EAL after its conserved residues. The EAL domain is a good candidate for a diguanylate phosphodiesterase function [1]. The domain contains many conserved acidic residues that could participate in metal binding and might form the phosphodiesterase active site.; Conserved hypothetical protein; EAL-domain-containing protein 360240 4606334 azo0335 Azoarcus sp. BH72 EAL-domain-containing protein YP_931839.1 359461 D 62928 CDS YP_931840.1 119896627 4606335 360237..361499 1 NC_008702.1 PAS/PAC/GGDEF-domain containing protein, suggesting involvement into signalling processes. Similarity to SWISSPROT: sprot|YDAM_ECOLI (24% Escherichia coli, hyp. protein) / TREMBL: trembl|Q887F0 (59% Pseudomonas syringae, hyp. protein) Pfam: PF00990 GGDEF domain. This domain is found linked to a wide range of non-homologous domains in a variety of bacteria. The function of this domain is unknown, however it has been shown to be homologous to the adenylyl cyclase catalytic domain. This prediction correlates with the functional information available on two GGDEF-containing proteins, namely diguanylate cyclase and phosphodiesterase A of Acetobacter xylinum, both of which regulate the turnover of cyclic diguanosine monophosphate. TIGRFAM:TIGR00254 putative diguanylate cyclase (GGDEF) domain. TIGR00229 PAS domain S-box.; Conserved hypothetical protein; PAS/PAC/GGDEF-domain-containing protein 361499 4606335 azo0336 Azoarcus sp. BH72 PAS/PAC/GGDEF-domain-containing protein YP_931840.1 360237 D 62928 CDS YP_931841.1 119896628 4606336 complement(361535..362107) 1 NC_008702.1 Probable hypothetical Protein. Extremely weak homology with hits in the PDB. No Motif/Features/TMH/ or Signal peptide present.; hypothetical protein 362107 4606336 azo0337 Azoarcus sp. BH72 hypothetical protein YP_931841.1 361535 R 62928 CDS YP_931842.1 119896629 4606337 362400..363443 1 NC_008702.1 38% Peri-phosph.IPR006059; SBP_bac_1. Pfam:PF01547; SBP_bac_1; 1. TIGRFAMs:TIGR00975; 3a0107s03; 1. Signal peptide present.; High confidence in function and specificity; putative phosphate-binding periplasmic protein 363443 pstS1 4606337 pstS1 Azoarcus sp. BH72 putative phosphate-binding periplasmic protein YP_931842.1 362400 D 62928 CDS YP_931843.1 119896630 4609643 complement(363495..363989) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to an orf of Acinetobacter sp. (strain ADP1) of 43% (tremble:Q6F6W4). no domains predicted. signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 363989 4609643 azo0339 Azoarcus sp. BH72 hypothetical protein YP_931843.1 363495 R 62928 CDS YP_931844.1 119896631 4606338 complement(364232..364546) 1 NC_008702.1 Conserved hypothetical membrane protein. No homology to the data bank No domains predicted No signal peptide 2 TMHs; hypothetical protein 364546 4606338 azo0340 Azoarcus sp. BH72 hypothetical protein YP_931844.1 364232 R 62928 CDS YP_931845.1 119896632 4606339 364615..365061 1 NC_008702.1 Putative cytochrome c'. Homology to cytochrome c of R. gelatineosus (sprot|CYCP_RHOGE). CYTOCHROME C IS THE MOST WIDELY OCCURRING BACTERIAL C-TYPE CYTOCHROME. CYTOCHROMES C ARE HIGH-SPIN PROTEINS AND THE HEME HAS NO SIXTH LIGAND. THEIR EXACT FUNCTION IS NOT KNOWN. InterPro: Cytochrome c class II (IPR002321) Pfam: Cytochrome C probable signal peptide no TMHs; Function unclear; putative cytochrome c' 365061 4606339 azo0341 Azoarcus sp. BH72 putative cytochrome c' YP_931845.1 364615 D 62928 CDS YP_931846.1 119896633 4606340 complement(365077..365901) 1 NC_008702.1 Belongs to the arylamine n-acetyltransferase family. Arylamine N-acetyltransferase is a cytosolic enzyme of approximately 30kDa. It facilitates the transfer of an acetyl group from Acetyl Coenzyme A on to a wide range of arylamine, N-hydroxyarylamines and hydrazines. Similar to TREMBL:Q98D42 (47% identity); TREMBL:Q8FHI6 (39% identity); SWISSPROT:P77567 (39% identity). InterPro (IPR001447): N-acetyltransferase Pfam (PF00797): N-acetyltransferase.; High confidence in function and specificity; N-hydroxyarylamine O-acetyltransferase 365901 nhoA 4606340 nhoA Azoarcus sp. BH72 N-hydroxyarylamine O-acetyltransferase YP_931846.1 365077 R 62928 CDS YP_931847.1 119896634 4609356 366042..366704 1 NC_008702.1 Sporulation initiation inhibitor protein soj. INHIBITS THE INITIATION OF SPORULATION SPO0J ANTAGONIZES THIS INHIBITION. SOJ ULTIMATELY INHIBITS THE ACTIVATION (PHOSPHORYLATION) OF SPO0A. IT IS NOT REQUIRED FOR CHROMOSOME PARTITIONING. InterPro: ParA family ATPase; Function unclear; ParA family protein 366704 parA2 4609356 parA2 Azoarcus sp. BH72 ParA family protein YP_931847.1 366042 D 62928 CDS YP_931848.1 119896635 4609498 366705..367574 1 NC_008702.1 Probable Hypothetical Protein. No domains,features,motifs, or signal peptide present.; hypothetical protein 367574 4609498 azo0344 Azoarcus sp. BH72 hypothetical protein YP_931848.1 366705 D 62928 CDS YP_931849.1 119896636 4606341 complement(367763..369865) 1 NC_008702.1 ATP-dependent DNA helicase pcrA (EC 3.6.1.-). InterPro: UvrD/REP helicase; Specificity unclear; ATP-dependent DNA helicase 369865 4606341 azo0345 Azoarcus sp. BH72 ATP-dependent DNA helicase YP_931849.1 367763 R 62928 CDS YP_931850.1 119896637 4606342 369965..371605 1 NC_008702.1 42% Na_H_porter.IPR004705; NaHantiport_bac.Bacterial Na+/H+ antiporter Pfam:PF00999; Na_H_Exchanger; 1. TIGRFAMs:TIGR00831; a_cpa1; 1. Signal peptide present TMHelix: 12; High confidence in function and specificity; putative Na+/H+ antiporter 371605 4606342 azo0346 Azoarcus sp. BH72 putative Na+/H+ antiporter YP_931850.1 369965 D 62928 CDS YP_931851.1 119896638 4606343 371653..372096 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. Signal P reporting Signal Peptide present. No TMH reported Present.; hypothetical protein 372096 4606343 azo0347 Azoarcus sp. BH72 hypothetical protein YP_931851.1 371653 D 62928 CDS YP_931852.1 119896639 4606344 372236..373684 1 NC_008702.1 Prenyltransferase family protein. InterPro: UbiA prenyltransferase; Specificity unclear; hypothetical protein 373684 4606344 azo0348 Azoarcus sp. BH72 hypothetical protein YP_931852.1 372236 D 62928 CDS YP_931853.1 119896640 4606345 373681..374046 1 NC_008702.1 Putative Small Multi-Drug resistant(SMR)family protein, 30% identical to TrEMBL;Q8XZS2. Has PF00893,Small Multidrug Resistance protein;IPR000390, Smr:This family is the Small Multidrug Resistance (SMR) family. Several members have been shown to export a range of toxins, including ethidium bromide and quaternary ammonium compounds, through coupling with proton influx.; putative small multi-drug resistant family protein 374046 4606345 azo0349 Azoarcus sp. BH72 putative small multi-drug resistant family protein YP_931853.1 373681 D 62928 CDS YP_931854.1 119896641 4606346 complement(374102..375397) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to gll2146 of G. violaceus of 44% (trembl|Q7NIN7(SRS)) no domains predicted signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 375397 4606346 azo0350 Azoarcus sp. BH72 hypothetical protein YP_931854.1 374102 R 62928 CDS YP_931855.1 119896642 4606347 complement(375522..376307) 1 NC_008702.1 Forms with NodI a membrane transport complex involved in the nodulation process. it probably exports a modified beta-1,4-linked n-acetylglucosamine oligosaccharide. Belongs to the ABC-2 integral membrane protein family, TREMBL:Q7WHD1 (49% identity); SWISSPROT:O52619 (49% identity). InterPro (IPR000412): ABC transporter family 2. Pfam (PF01061): ABC-2 type transporter. TMHMM reporting six transmembrane helices. TC (3.A.1.102): The Lipooligosaccharide Exporter (LOSE) Family.; High confidence in function and specificity; ABC transporter permease NodJ 376307 nodJ 4606347 nodJ Azoarcus sp. BH72 ABC transporter permease NodJ YP_931855.1 375522 R 62928 CDS YP_931856.1 119896643 4609391 complement(376345..376629) 1 NC_008702.1 Conserved hypothetical protein. Homology to mvpT of E. carotovora of 44% (trembl:Q6D393) No domains predicted. No TMHs No signal peptide; hypothetical protein 376629 4609391 azo0352 Azoarcus sp. BH72 hypothetical protein YP_931856.1 376345 R 62928 CDS YP_931857.1 119896644 4606348 complement(376663..376758) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to the data bank. No domains predicted. No signal peptide No TMHs; hypothetical protein 376758 4606348 azo0353 Azoarcus sp. BH72 hypothetical protein YP_931857.1 376663 R 62928 CDS YP_931858.1 119896645 4606349 377176..378156 1 NC_008702.1 Region start changed from 377491 to 377176 (315 bases); putative methylase 378156 4606349 azo0354 Azoarcus sp. BH72 putative methylase YP_931858.1 377176 D 62928 CDS YP_931859.1 119896646 4606350 complement(378157..379323) 1 NC_008702.1 Conserved hypothetical protein. Homology to lpg1234 of L.pneumophila of 42% (gnl|keqq|lpn:lpg1234(KEGG)). Has PF04471, Restriction endonuclease;IPR007560, Mrr_cat; Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA. No TMHs. No signal peptide.; hypothetical protein 379323 4606350 azo0355 Azoarcus sp. BH72 hypothetical protein YP_931859.1 378157 R 62928 CDS YP_931860.1 119896647 4606351 complement(379320..379709) 1 NC_008702.1 Putative hypothetical very short patch repair endonuclease. Homology to vrs of E. coli of 29% (sprot|VSR_ECOLI) All proteins in this family for which functions are known are G:T mismatch endonucleases that function in a specialized mismatch repair process used usually to repair G:T mismatches in specific sections of the genome. Interpro: DNA mismatch endonuclease vsr (IPR004603) Pfam: DNA mismatch endonuclease Vsr Tigrfam: vsr: DNA mismatch endonuclease no signal peptide no TMHs; Family membership; very short patch repair endonuclease 379709 vsr 4606351 vsr Azoarcus sp. BH72 very short patch repair endonuclease YP_931860.1 379320 R 62928 CDS YP_931861.1 119896648 4610095 complement(379781..380920) 1 NC_008702.1 hypothetical protein 380920 4610095 azo0357 Azoarcus sp. BH72 hypothetical protein YP_931861.1 379781 R 62928 CDS YP_931862.1 119896649 4606352 complement(380934..383189) 1 NC_008702.1 Region start changed from 382943 to 383189 (246 bases); putative two-component sensor kinase 383189 4606352 azo0358 Azoarcus sp. BH72 putative two-component sensor kinase YP_931862.1 380934 R 62928 CDS YP_931863.1 119896650 4606353 complement(383349..384059) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to MS2110 of M.succiniciproducens of 40% (gi|52426165|ref|YP_089302.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. 1 TMHs; Family membership; hypothetical protein 384059 4606353 azo0359 Azoarcus sp. BH72 hypothetical protein YP_931863.1 383349 R 62928 CDS YP_931864.1 119896651 4606354 complement(384192..385178) 1 NC_008702.1 Nod factor export ATP-binding protein. Part of the ABC transporter complex nodIJ (TC 3.A.1.102.1) involved in the export of LCO (lipo-chitin oligosaccharide) and a modified beta-14-linked N- acetylglucosamine oligosaccharide. Responsible for energy coupling to the transport system, TREMBL:Q7W9D3 (58% identity); SWISSPROT:O52618 (53% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003593): AAA ATPase. Pfam (PF00005): ABC transporter. TC (3.A.1.102) The Lipooligosaccharide Exporter (LOSE) Family.; High confidence in function and specificity; ABC transporter ATP-binding protein 385178 nodI 4606354 nodI Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_931864.1 384192 R 62928 CDS YP_931865.1 119896652 4609390 complement(385249..385866) 1 NC_008702.1 Conserved hypothetical glutathione S-transferase. Homology to cv3306 of C. violaceum of 50% (trembl|Q7NSW3). Conjugation of reduced glutathione to a variety of targets. Pfam: Glutathione S-transferase, N-terminal domain; Glutathione S-transferase, C-terminal domain. no signal peptide. no TMHs; Conserved hypothetical protein; glutathione S-transferase 385866 4609390 azo0361 Azoarcus sp. BH72 glutathione S-transferase YP_931865.1 385249 R 62928 CDS YP_931866.1 119896653 4606355 complement(385891..386562) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc0328 of R. solanacearum of 59% (trembl|Q8Y2K7). Pfam: Prolyl oligopeptidas family no signal peptide no TMHs; hypothetical protein 386562 4606355 azo0362 Azoarcus sp. BH72 hypothetical protein YP_931866.1 385891 R 62928 CDS YP_931867.1 119896654 4606356 complement(386572..386892) 1 NC_008702.1 Putative ferredoxin 2fe-2s protein. Homology to fer2 of C. pasteruianum of 35% (sprot|FER2_CLOPA). Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. no signal peptide no TMHs; Family membership; putative ferredoxin 2Fe-2S protein 386892 fer21 4606356 fer21 Azoarcus sp. BH72 putative ferredoxin 2Fe-2S protein YP_931867.1 386572 R 62928 CDS YP_931868.1 119896655 4608757 complement(386915..388000) 1 NC_008702.1 Conserved hypothtical membrane protein. Homology to rc03284 of R. solanacearum of 35% (trembl|Q8Y2L8(SRS) Has a weak hit to PF04892(IPR006976;vanZ)in Smart:This family contains several examples of the VanZ protein, but also contains examples of phosphotransbutyrylases. VanZ confers low-level resistance to the glycopeptide antibiotic teicoplanin (Te). Analysis of cytoplasmic peptidoglycan precursors, accumulated in the presence of ramoplanin,showed that VanZ-mediated Te resistance does not involve incorporation of a substituent of D-alanine into the peptidoglycan precursors . no signal peptide 10 TMHs; Conserved hypothetical protein; hypothetical protein 388000 4608757 azo0364 Azoarcus sp. BH72 hypothetical protein YP_931868.1 386915 R 62928 CDS YP_931869.1 119896656 4606357 388715..389227 1 NC_008702.1 Conserved Hypothetical protein, Q82Y48,Rhodanese type protein. Has SMART SM00450 Rhodanese Homology Domain(RHOD)starting at position 47-153aa;Rhodanese, a sulfurtransferase involved in cyanide detoxification (see IPR001307) shares evolutionary relationship with a large family of proteins. No Signal peptide or TMH reported Present.; Family membership; hypothetical protein 389227 4606357 azo0365 Azoarcus sp. BH72 hypothetical protein YP_931869.1 388715 D 62928 CDS YP_931870.1 119896657 4606358 389292..390254 1 NC_008702.1 Probable serine O-acetyltransferase plasmid (EC 2.3.1.30) (SAT). Homology to srpH of Synechococcus of 55% (sprot|SRPH_SYNP7) no signal peptide no TMHs; High confidence in function and specificity; serine O-acetyltransferase 390254 srpH 4606358 srpH Azoarcus sp. BH72 serine O-acetyltransferase YP_931870.1 389292 D 62928 CDS YP_931871.1 119896658 4609891 390251..390484 1 NC_008702.1 SgrAlc control protein,; High confidence in function and specificity; SgrAlc control protein 390484 4609891 azo0367 Azoarcus sp. BH72 SgrAlc control protein YP_931871.1 390251 D 62928 CDS YP_931872.1 119896659 4606359 390602..391528 1 NC_008702.1 Probable immunodominant 35 kDa protein. Homology to mmpI of Mycobacterium avium of 69% (gi|2498566|sp|Q48899|MMP1_MYCAV(SwissProt (ExPASy)). No domains predicted. No signal peptide. No TMHs.,; High confidence in function and specificity; immunodominant 35kDa protein 391528 mmpI 4606359 mmpI Azoarcus sp. BH72 immunodominant 35kDa protein YP_931872.1 390602 D 62928 CDS YP_931873.1 119896660 4609230 391503..393335 1 NC_008702.1 Cysteine desulphurases required for the mobilization of sulphur from cysteine. They are present in all organisms, where they are involved in iron-sulphur (Fe-S) cluster biosynthesis. Similar to sprot|CSD1_MYCLE (54%), to trembl|Q82WT8 (55%) and to tremblnew|BAB21542 (34%). Pfam (PF00266): Aminotransferase class-V Pfam (PF01041): DegT/DnrJ/EryC1/StrS aminotransferase family; Function unclear; cysteine desulfurase 393335 csdB 4609230 csdB Azoarcus sp. BH72 cysteine desulfurase YP_931873.1 391503 D 62928 CDS YP_931874.1 119896661 4608537 393595..394170 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE2493 of Nitrosomonas europaea of 67% (trembl|Q82S65(SRS)). Has PF01169, Uncharacterized protein family UPF0016;IPR001727; This family contains integral membrane proteins of unknown function. Most members of the family contain two copies of a region that contains an EXGD motif. No signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 394170 4608537 azo0370 Azoarcus sp. BH72 hypothetical protein YP_931874.1 393595 D 62928 CDS YP_931875.1 119896662 4606360 394317..395381 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 395381 4606360 azo0371 Azoarcus sp. BH72 hypothetical protein YP_931875.1 394317 D 62928 CDS YP_931876.1 119896663 4606361 complement(395413..395931) 1 NC_008702.1 Entry name SWISSPROT:YHBT_ECOLI Prim. accession # P45474 InterPro IPR003033; SCP2. Pfam PF02036; SCP2; 1. Prediction: Non-secretory protein Signal peptide probability: 0.001 Number of predicted TMHs: 0 Identities = 46/107 (42%); hypothetical protein 395931 4606361 azo0372 Azoarcus sp. BH72 hypothetical protein YP_931876.1 395413 R 62928 CDS YP_931877.1 119896664 4606362 complement(395946..396842) 1 NC_008702.1 Conserved hypothetical peptidase. Homology to Rsp0969 of R. solanacearum of 51% (trembl|Q8XR90). Pfam: Peptidas family U32 no signal peptide no TMHs; Family membership; peptidase 396842 4606362 azo0373 Azoarcus sp. BH72 peptidase YP_931877.1 395946 R 62928 CDS YP_931878.1 119896665 4606363 complement(396857..397870) 1 NC_008702.1 Conserved hypothetical peptidase. Homology to cv4084 of C. violaceum of 64% (trembl|Q7NQQ39. Pfam: Peptidas family U32 no signal peptide no TMHs; Family membership; peptidase 397870 4606363 azo0374 Azoarcus sp. BH72 peptidase YP_931878.1 396857 R 62928 CDS YP_931879.1 119896666 4606364 complement(398061..399524) 1 NC_008702.1 TREMBL:Q8Y273: 59% identity, 72% similarity Osmoregulated proline transporter (Sodium/proline symporter). CATALYZES THE SODIUM-DEPENDENT UPTAKE OF EXTRACELLULAR PROLINE. InterPro: Sodium:solute symporter family InterPro:IPR001734; Na/solut_symport. Pfam:PF00474; SSF; TMHMM predicted transmembrane helices sss: SSS sodium solute transporter sup; Function unclear; sodium/solute symporter transmembrane protein 399524 4606364 azo0375 Azoarcus sp. BH72 sodium/solute symporter transmembrane protein YP_931879.1 398061 R 62928 CDS YP_931880.1 119896667 4606365 complement(399721..401841) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3609 of Azoarcus sp. EbN1 of 62% (gnl|keqq|eba:ebA3609(KEGG)). InterPro: Aminotransferase class-III (IPR005814), Arginase family (IPR00594). Pfam: Aminotransferase class-III,Arginase family. Tigrfam: argD: acetylornithine and succinylornithine aminotransferase, GABAtrnsam:4-aminobutyrate aminotransferase. no signal peptide. no TMHs; hypothetical protein 401841 4606365 azo0376 Azoarcus sp. BH72 hypothetical protein YP_931880.1 399721 R 62928 CDS YP_931881.1 119896668 4606366 complement(401938..403467) 1 NC_008702.1 an Escherichia coli mutant results in accumulation of octaprenylphenol and no ubiquinone; functions in the formation of 2-octaprenyl-6-hydroxy-phenol from 2-octaprenylphenol in ubiquinone (coenzyme Q) biosynthesis; similar to eukaryotic proteins that exhibit kinase functions; putative ubiquinone biosynthesis protein UbiB 403467 ubiB 4606366 ubiB Azoarcus sp. BH72 putative ubiquinone biosynthesis protein UbiB YP_931881.1 401938 R 62928 CDS YP_931882.1 119896669 4606367 complement(403712..405787) 1 NC_008702.1 Putative TonB-dependent receptor. Homology to bpp0186 ao B. parapertussis of 54% (trembl|Q7W206) The TonB protein interacts with outer membrane receptor proteins that carry out high-affinity binding and energy-dependent uptake of specific substrates into the periplasmic space. These substrates are either poorly permeable through the porin channels or are encountered at very low concentrations. In the absence of TonB these receptors bind their substrates but do not carry out active transport. Interpro: TonB-dependent receptor (IPR000531) Pfam: TonB-dependent receptor (PF00593) signal Peptide no TMHs; Family membership; putative TonB-dependent receptor 405787 4606367 azo0378 Azoarcus sp. BH72 putative TonB-dependent receptor YP_931882.1 403712 R 62928 CDS YP_931883.1 119896670 4606368 complement(405969..406301) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. signal peptide. No TMHs; hypothetical protein 406301 4606368 azo0379 Azoarcus sp. BH72 hypothetical protein YP_931883.1 405969 R 62928 CDS YP_931884.1 119896671 4606369 406538..408301 1 NC_008702.1 catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase; arginyl-tRNA synthetase 408301 argS 4606369 argS Azoarcus sp. BH72 arginyl-tRNA synthetase YP_931884.1 406538 D 62928 CDS YP_931885.1 119896672 4608341 408318..408902 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV3999 of C.violaceum of 34% (trembl|Q7NQY6(SRS)). Pfam: Sporulation related repeat This 35 residue repeat is found in proteins involved in sporulation and cell division such as FtsN, DedD, and CwlM. This repeat might be involved in binding peptidoglycan (Bateman A pers obs). No TMHs. Signal peptide present; Conserved hypothetical protein; hypothetical protein 408902 4608341 azo0381 Azoarcus sp. BH72 hypothetical protein YP_931885.1 408318 D 62928 CDS YP_931886.1 119896673 4606370 408982..409629 1 NC_008702.1 Putative protein disulfide-isomerase. Homology to dsbA of P. flourescens of 34%. Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins. Tigrfam: redox_disulf_1: redox-active disulfid Pfam: DSBA Oxidoreductase signal peptide no TMHS; Family membership; putative protein disulfide-isomerase 409629 dsbA 4606370 dsbA Azoarcus sp. BH72 putative protein disulfide-isomerase YP_931886.1 408982 D 62928 CDS YP_931887.1 119896674 4608653 409658..410446 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8Y2Q1 (42% identity); SWISSPROT:P25970 (34% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short chain dehydrogenase 410446 4608653 azo0383 Azoarcus sp. BH72 short chain dehydrogenase YP_931887.1 409658 D 62928 CDS YP_931888.1 119896675 4606371 410530..410949 1 NC_008702.1 Hypothetical protein. No Good homologs in the DB matching the length of the protein. No Significant domains,features,motifs present.; hypothetical protein 410949 4606371 azo0384 Azoarcus sp. BH72 hypothetical protein YP_931888.1 410530 D 62928 CDS YP_931889.1 119896676 4606372 410991..412268 1 NC_008702.1 Hypothetical membrane protein. Homology to vng2292h of Halobacterium sp. of 25% (trembl|Q9HN16(SRS). no domains predicted .no signal peptide. 10 TMHs; hypothetical protein 412268 4606372 azo0385 Azoarcus sp. BH72 hypothetical protein YP_931889.1 410991 D 62928 CDS YP_931890.1 119896677 4606373 412265..413521 1 NC_008702.1 Hypothetical membrane protein. No good homology to a protein of similar length in the data bank. no domains predicted no singal peptide 9 TMHs; hypothetical protein 413521 4606373 azo0386 Azoarcus sp. BH72 hypothetical protein YP_931890.1 412265 D 62928 CDS YP_931891.1 119896678 4606374 413530..414396 1 NC_008702.1 Conserved hypothetical periplasmic binding proten. Homology to rs03250 of R. solanacearum of 53% (trembl|Q8Y2Q2). Interpro: Periplasmic binding protein (IPR002491). Pfam: Periplasmic binding protein (PF01497). This family includes bacterial periplasmic binding proteins. Several of which are involved in iron transport. no signal peptide. no TMHs.; Conserved hypothetical protein; periplasmic binding protein 414396 4606374 azo0387 Azoarcus sp. BH72 periplasmic binding protein YP_931891.1 413530 D 62928 CDS YP_931892.1 119896679 4606375 complement(414428..414877) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3626 of Azoarcus sp. EbN1 of 59% (gnl|keqq|eba:ebA3626(KEGG)). InterPro: Protein of unknown function DUF132 InterPro:IPR002850; DUF132. IPR002716; PIN. IPR006596; PINc. Pfam:PF01850; PIN; SMART:SM00670; PINc; TIGRFAMs:TIGR00305; DUF132 TIGR00305: conserved hypothetical protein T. Non-secretory protein with very low signalpeptide probability (0.003) (Signal P predicted). Absence of transmembrane helices; hypothetical protein 414877 4606375 azo0388 Azoarcus sp. BH72 hypothetical protein YP_931892.1 414428 R 62928 CDS YP_931893.1 119896680 4606376 complement(414946..416775) 1 NC_008702.1 Function:-Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation. Entry name TREMBL:Q8KXD5 Prim. accession # Q8KXD5 Identities = 354/607 (58%) InterPro IPR000073; A/b_hydrolase. IPR010941; PhaC_N. Pfam PF00561; Abhydrolase_1; 1. PF07167; PhaC_N; 1. Number of predicted TMHs: 0; High confidence in function and specificity; poly-beta-hydroxybutyrate synthase 416775 phbC1 4606376 phbC1 Azoarcus sp. BH72 poly-beta-hydroxybutyrate synthase YP_931893.1 414946 R 62928 CDS YP_931894.1 119896681 4609541 complement(416816..417844) 1 NC_008702.1 conserved hypothetical protein. TREMBL:Q8Y2Q4-47% identity, 61% similarity. Pfam: Lactamase_B,aminotransferase III, AP2 domain. TMHMM predicted transmembrane helices.; Specificity unclear; hypothetical protein 417844 4609541 azo0390 Azoarcus sp. BH72 hypothetical protein YP_931894.1 416816 R 62928 CDS YP_931895.1 119896682 4606377 417987..418430 1 NC_008702.1 Putative MerR-family transcriptional regulator,copper efflux regulator / copper export regulator) InterPro: IPR000551 HTH_MerR. HTH reporting nucleic acid binding motif.; Family membership; MerR family transcriptional regulator 418430 4606377 azo0391 Azoarcus sp. BH72 MerR family transcriptional regulator YP_931895.1 417987 D 62928 CDS YP_931896.1 119896683 4606378 418560..419027 1 NC_008702.1 Similar to TREMBL:Q8YBL0 (47% identity); TREMBL:Q987R7 (50% identity); TREMBL:Q7WA35 (55% identity). InterPro (IPR003736): Phenylacetic acid degradation-related protein. Pfam (DUF157): Uncharacterized protein PaaI, COG2050.; Function unclear; hypothetical protein 419027 4606378 azo0392 Azoarcus sp. BH72 hypothetical protein YP_931896.1 418560 D 62928 CDS YP_931897.1 119896684 4606379 419104..420315 1 NC_008702.1 Probable acyl-CoA thiolase. Homology to fadAx of P. putida of 67% (gnl|keqq|ppu:PP2215(KEGG)). IPR002155; Thiolase. Pfam PF02803; Thiolase_C; 1. PF00108; Thiolase_N; 1. Two different types of thiolase are found both in eukaryotes and in prokaryotes: acetoacetyl-CoA thiolase (EC:2.3.1.9) and 3-ketoacyl-CoA thiolase (EC:2.3.1.16). 3-ketoacyl-CoA thiolase (also called thiolase I) has a broad chain-length specificity for its substrates and is involved in degradative pathways such as fatty acid -oxidation. Acetoacetyl-CoA thiolase (also called thiolase II) is specific for the thiolysis of acetoacetyl-CoA and involved in biosynthetic pathways such as poly -hydroxybutyrate synthesis or steroid biogenesis.Prediction: Non-secretory protein Signal peptide probability: 0.002 Number of predicted TMHs: 0; Specificity unclear; acyl-CoA thiolase 420315 fadAx 4606379 fadAx Azoarcus sp. BH72 acyl-CoA thiolase YP_931897.1 419104 D 62928 CDS YP_931898.1 119896685 4608719 420508..421641 1 NC_008702.1 Activity:- Catalysis of the reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Entry name TREMBL:Q9AHX9 Prim. accession # Q9AHX9 InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; 1. Identities = 244/375 (65%) Number of predicted TMHs: 0; Family membership; acyl-CoA dehydrogenase 421641 fadfX 4608719 fadfX Azoarcus sp. BH72 acyl-CoA dehydrogenase YP_931898.1 420508 D 62928 CDS YP_931899.1 119896686 4608727 421777..422394 1 NC_008702.1 Similar to TREMBL:Q8Y2R2 (42% identity); TREMBL:Q7WEK4 (30% identity); TREMBL:Q88IW7 (27% identity). Pfam (HCCA_isomerase): 2-hydroxychromene-2-carboxylate isomerase.; Family membership; 2-hydroxychromene-2-carboxylate isomerase 422394 4608727 azo0395 Azoarcus sp. BH72 2-hydroxychromene-2-carboxylate isomerase YP_931899.1 421777 D 62928 CDS YP_931900.1 119896687 4606380 422445..422642 1 NC_008702.1 Conserved hypothetical protein. Homology to cc1308 of C. crescentus of 35% (trembl|Q9A8P5). Pfam:DUF1289 This family consists of a number of hypothetical bacterial proteins. The aligned region spans around 56 residues and contains 4 highly conserved cysteine residues towards the N-terminus. The function of this family is unknown. no signal peptide no TMHs; hypothetical protein 422642 4606380 azo0396 Azoarcus sp. BH72 hypothetical protein YP_931900.1 422445 D 62928 CDS YP_931901.1 119896688 4606381 422639..422824 1 NC_008702.1 Hypothetical protein, 54% identity to TrEMBl;Q8YGQ7. Has PF06945, Protein of unknown function (DUF1289);IPR010710;This family consists of a number of hypothetical bacterial proteins. The aligned region spans around 56 residues and contains 4 highly conserved cysteine residues towards the N-terminus. The function of this family is unknown.; hypothetical protein 422824 4606381 azo0397 Azoarcus sp. BH72 hypothetical protein YP_931901.1 422639 D 62928 CDS YP_931902.1 119896689 4606382 422821..423738 1 NC_008702.1 Probable Hypothetical protein ycbL. TREMBL:Q8Y2S7-43% identity,57% similarity. TIGRFAM:2A0115-Benzoate transport proteins belong to this group. Benzyl alcohol,benzaldehyde, benzoate, and anthranilate are metabolized via catechol, cis,cis-muconate, and the beta-ketoadipate pathway in some bacteria InterPro: Metallo-beta-lactamase superfamily Pfam:Metallo Beta lactamase superfamily, Phage lysozyme TMHMM predicted transmembrane heleces ftsZ: cell division protein FtsZ; Function unclear; putative beta lactamase 423738 4606382 azo0398 Azoarcus sp. BH72 putative beta lactamase YP_931902.1 422821 D 62928 CDS YP_931903.1 119896690 4606383 423811..424689 1 NC_008702.1 Hypothetical UPF0042 protein YPO3586/Y0158. SWISPROT:Q82TN5: 6i% identity, 78% similarity This is a family of putative P-loop ATPases. Many of the proteins in this family are hypothetical and kinase activity has been proposed for some family members InterPro:IPR005337; UPF0042. Pfam: PF03668; ATP_bind_2 No Signal peptide (Signal P predicted) No transmembrane helices (TMHMM predicted) cmk: cytidylate kinase; Function unclear; hypothetical protein 424689 4606383 azo0399 Azoarcus sp. BH72 hypothetical protein YP_931903.1 423811 D 62928 CDS YP_931904.1 119896691 4606384 424686..425081 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to TP1032 of Treponema pallidum of 32% (sprot|YA32_TREPA(SRS)). Has PF07009(IPR010739):Protein of unknown function (DUF1312);This family consists of several bacterial proteins of around 120 residues in length. The function of this family is unknown. No singal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 425081 4606384 azo0400 Azoarcus sp. BH72 hypothetical protein YP_931904.1 424686 D 62928 CDS YP_931905.1 119896692 4606385 425062..425604 1 NC_008702.1 Heptaprenyl diphosphate synthase component I, 23% identity (45% similarity) to TrEMBL;Q896H9. Has PF07456,Heptaprenyl diphosphate synthase component I;IPR010898,Hpre_diP_synt_I: This family contains component I of bacterial heptaprenyl diphosphate synthase (approximately 170 residues long). This is one of the two dissociable subunits that form the enzyme, both of which are required for the catalysis of the biosynthesis of the side chain of menaquinone-7.; heptaprenyl diphosphate synthase component I 425604 4606385 azo0401 Azoarcus sp. BH72 heptaprenyl diphosphate synthase component I YP_931905.1 425062 D 62928 CDS YP_931906.1 119896693 4606386 complement(425612..426709) 1 NC_008702.1 regulates chemotaxis by demethylation of methyl-accepting chemotaxis proteins; chemotaxis-specific methylesterase 426709 cheB1 4606386 cheB1 Azoarcus sp. BH72 chemotaxis-specific methylesterase YP_931906.1 425612 R 62928 CDS YP_931907.1 119896694 4608467 complement(426736..427560) 1 NC_008702.1 Probable chemotaxis protein methyltransferase,CheR_Metranf. IPR001601; Methyltransf. IPR000051; SAM_bind. Pfam: PF01739; CheR. PF03705; CheR_N. SMART: SM00138; MeTrc. Chemotaxis protein methyltransferase (EC 2.1.1.80). METHYLATION OF THE MEMBRANE-BOUND METHYL-ACCEPTING CHEMOTAXIS PROTEINS (MCP) TO FORM GAMMA-GLUTAMYL METHYL ESTER RESIDUES IN MCP.Annotation derived from meta auto annotator.; High confidence in function and specificity; chemotaxis protein methyltransferase 427560 cheR1 4608467 cheR1 Azoarcus sp. BH72 chemotaxis protein methyltransferase YP_931907.1 426736 R 62928 CDS YP_931908.1 119896695 4608470 complement(427566..428156) 1 NC_008702.1 Conserved hypothetical chemotaxis protein CheD. Homology to cheD of S. oneidensis of 41% (trembl|Q8EF62). Interpro: CheD (IPR005659). Pfam: CheD (PF03975). This chemotaxis protein stimulates methylation of MCP proteins. no signal peptide. no TMHs; Conserved hypothetical protein; chemotaxis protein CheD 428156 cheD 4608470 cheD Azoarcus sp. BH72 chemotaxis protein CheD YP_931908.1 427566 R 62928 CDS YP_931909.1 119896696 4608469 complement(428223..428765) 1 NC_008702.1 Probable positive regulator of CheA protein activity, CheW. Pfam: PF01584; CheW. SMART: SM00260; CheW. Chemotaxis protein cheW.Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges chea to the mcps (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to cheY and cheB.; High confidence in function and specificity; positive regulator of CheA protein activity 428765 cheW1 4608469 cheW1 Azoarcus sp. BH72 positive regulator of CheA protein activity YP_931909.1 428223 R 62928 CDS YP_931910.1 119896697 4608474 complement(428787..431225) 1 NC_008702.1 Methyl-accepting chemotaxis protein, only very low similarity to SWISSPROT: sprot|Y4FA_RHISN (14% Rhizobium sp. (strain NGR234). InterPro: IPR004090; Me_chemotaxis. IPR004089; Chmtaxis_transd. IPR003660; HAMP. Pfam: PF00672; HAMP. PF00015; MCPsignal. SMART: SM00304; HAMP. SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Family membership; putative methyl-accepting chemotaxis protein 431225 4608474 azo0406 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_931910.1 428787 R 62928 CDS YP_931911.1 119896698 4606387 complement(431253..433700) 1 NC_008702.1 Methyl-accepting chemotaxis protein, only very low similarity to SWISSPROT: sprot|Y4FA_RHISN (14% Rhizobium sp. (strain NGR234). InterPro: IPR004090; Me_chemotaxis. IPR004089; Chmtaxis_transd. IPR003660; HAMP. Pfam: PF00672; HAMP. PF00015; MCPsignal. SMART: SM00304; HAMP. SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 1 transmembrane helices.; Family membership; putative methyl-accepting chemotaxis protein 433700 4606387 azo0407 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_931911.1 431253 R 62928 CDS YP_931912.1 119896699 4606388 complement(433738..435933) 1 NC_008702.1 Putative chemotaxis histidine kinase protein,ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR002545; CheW. IPR004105; H-kinase_dim. IPR005467; His_kinase. IPR008207; Hpt. IPR008208; Hpt_N. Pfam: PF01584; CheW. PF02895; H-kinase_dim. PF02518; HATPase_c. PF01627; Hpt. SMART: SM00260; CheW. SM00387; HATPase_c. SM00073; HPT. Chemotaxis protein cheA (EC 2.7.3.-). INVOLVED IN THE TRANSMISSION OF SENSORY SIGNALS FROM THE CHEMORECEPTORS TO THE FLAGELLAR MOTORS. CHEA IS AUTOPHOSPHORYLATED; IT CAN TRANSFER ITS PHOSPHATE GROUP TO EITHER CHEB OR CHEY.; High confidence in function and specificity; putative chemotaxis protein histidine kinase 435933 cheA1 4606388 cheA1 Azoarcus sp. BH72 putative chemotaxis protein histidine kinase YP_931912.1 433738 R 62928 CDS YP_931913.1 119896700 4608464 complement(435947..436255) 1 NC_008702.1 Putative anti-sigma factor antagonists (STAS domain protein), STAS. Pfam: PF01740; STAS. The STAS (Sulphate Transporter and AntiSigma factor antagonist) domain is found in the C-terminal region of sulphate transporters and bacterial anti-sigma factor antagonists. It has been suggested that this domain may have a general NTP binding function.; Function unclear; STAS domain-containing protein 436255 4608464 azo0409 Azoarcus sp. BH72 STAS domain-containing protein YP_931913.1 435947 R 62928 CDS YP_931914.1 119896701 4606389 complement(436258..437526) 1 NC_008702.1 Putative methyl-accepting chemotaxis protein,Chmtaxis_transd. Pfam: PF00015; MCPsignal. SMART: SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Function unclear; putative methyl-accepting chemotaxis protein 437526 4606389 azo0410 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_931914.1 436258 R 62928 CDS YP_931915.1 119896702 4606390 complement(437534..437899) 1 NC_008702.1 Probable chemotaxis response regulator,; High confidence in function and specificity; chemotaxis response regulator 437899 cheY1 4606390 cheY1 Azoarcus sp. BH72 chemotaxis response regulator YP_931915.1 437534 R 62928 CDS YP_931916.1 119896703 4610008 complement(438349..439440) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc0739 of R. solanacearum of 52% (trembl|Q8Y1F1). Pfam: DUF395 This family includes YeeE and YedE from E. coli. These proteins are integral membrane proteins of unknown function. Many of these proteins contain two homologous regions that are represented by this family. This region contains several conserved glycines and an invariant cysteine that is probably an important functional residue. signal peptide 8 TMHs; Conserved hypothetical protein; hypothetical protein 439440 4610008 azo0412 Azoarcus sp. BH72 hypothetical protein YP_931916.1 438349 R 62928 CDS YP_931917.1 119896704 4606391 complement(439455..440141) 1 NC_008702.1 Conserved hypothetical ExsB protein. Homology to exsB of P. syringae of 70% (trembl|Q87Y44). This protein family is represented by a single member in nearly every completed large (> 1000 genes) prokaryotic genome. In Rhizobium meliloti, a species in which the exo genes make succinoglycan, a symbiotically important exopolysaccharide, exsB is located nearby and affects succinoglycan levels, probably through polar effects on exsA expression or the same polycistronic mRNA. Interpro: ExsB (IPR004479). Pfam: ExsB (PF06508). Tigrfam: TIGR00364: exsB protein. Has signal peptide present. No TMHs; Family membership; ExsB protein 440141 exsB 4606391 exsB Azoarcus sp. BH72 ExsB protein YP_931917.1 439455 R 62928 CDS YP_931918.1 119896705 4608707 complement(440170..440832) 1 NC_008702.1 Conserved hypothetical radical activating enzyme. Homology to xac3139 of X. axonopodis of 60% (trembl|Q8PHW0). InterPro: Radical activating enzymes (IPR001989) Pfam: Radical activating enzyme no signal peptide no TMHs; Family membership; putative radical activating enzyme 440832 4608707 azo0414 Azoarcus sp. BH72 putative radical activating enzyme YP_931918.1 440170 R 62928 CDS YP_931919.1 119896706 4606392 complement(440843..441562) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS05116 of R.solanacearum of 38% (trembl|Q8Y1F3(SRS)) No domains predicted. No TMHs Has signal peptide.; Conserved hypothetical protein; hypothetical protein 441562 4606392 azo0415 Azoarcus sp. BH72 hypothetical protein YP_931919.1 440843 R 62928 CDS YP_931920.1 119896707 4606393 complement(441562..442080) 1 NC_008702.1 Putative peptidoglycan-associated lipoprotein. Homology to pal of E. coli of 36% (sprot|PAL_ECOLI). Thought to play a role in bacterial envelope integrity. Very strongly associated with the peptidoglycan. Pfam: ompA family signal peptide no TMHs; Family membership; putative peptidoglycan-associated lipoprotein 442080 pal 4606393 pal Azoarcus sp. BH72 putative peptidoglycan-associated lipoprotein YP_931920.1 441562 R 62928 CDS YP_931921.1 119896708 4609491 complement(442120..443403) 1 NC_008702.1 forms dimers; may be involved in cell envelope integrity; interacts with outer membrane proteins and with the C-terminal domain of inner membrane protein TolA; translocation protein TolB 443403 tolB 4609491 tolB Azoarcus sp. BH72 translocation protein TolB YP_931921.1 442120 R 62928 CDS YP_931922.1 119896709 4609958 complement(443450..444292) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to tolA of N. europaea of 39% (trembl|Q82XP0). no domains predicted InterPro: Proline-rich region (IPR000694) no signal peptide 1 TMHs; Conserved hypothetical protein; hypothetical protein 444292 tolA 4609958 tolA Azoarcus sp. BH72 hypothetical protein YP_931922.1 443450 R 62928 CDS YP_931923.1 119896710 4609957 complement(444289..444702) 1 NC_008702.1 Putative translocation protein TolR. Holomology to tolR of P. aeruginosa of 30% (sprot|TOLR_PSEAE) Involved in eth TonB-independent uptake of proteins. no signal peptide probable 1 TMH; Family membership; putative translocation protein TolR 444702 tolR 4609957 tolR Azoarcus sp. BH72 putative translocation protein TolR YP_931923.1 444289 R 62928 CDS YP_931924.1 119896711 4609961 complement(444721..445395) 1 NC_008702.1 Translocation protein TolQ. Homology to tolQ of R. solanacearum of 52%. Involved in the tonB-independent uptake of proteins. InterPro: MotA/TolQ/ExbB proton channel family Pfam: MotA/TolQ/ExbB proton channel family no signal peptide probable 3 TMHs; High confidence in function and specificity; translocation protein TolQ 445395 tolQ 4609961 tolQ Azoarcus sp. BH72 translocation protein TolQ YP_931924.1 444721 R 62928 CDS YP_931925.1 119896712 4609960 complement(445392..445859) 1 NC_008702.1 Conserved hypothetical thioesterase. Homology to bb4233 of B. bronchiseptica of 41% (trembl|Q7WFN8). Tigrfam: TIGR00051: conserved hypothetical protein .Pfam: 4-hydroxybenzol-CoA thioesterase (PF0361). Interpro: 4-hydroxybenzol-CoA thioesterase (IPR006684); thioesterase superfamily (IPR006683) This family contains a wide variety of enzymes, principally thioesterases. This family includes 4HBT which catalyses the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3. This family includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyse the hydrolysis of other long chain fatty acyl-CoA thioesters. no signal peptide. no TMHs; Family membership; putative thioesterase 445859 4609960 azo0421 Azoarcus sp. BH72 putative thioesterase YP_931925.1 445392 R 62928 CDS YP_931926.1 119896713 4606394 445887..447230 1 NC_008702.1 SPROT:P37718: 26% identity, 43% similarity Cellulose synthase operon protein C precursor. Required for maximal bacterial cellulose synthesis. InterPro: STAS domain. The STAS (Sulphate Transporter and AntiSigma factor antagonist) domain is found in the C-terminal region of sulphate transporters and bacterial anti-sigma factor antagonists. It has been suggested that this domain may have a general NTP binding function. InterPro:IPR008410; BCSC_C. IPR003921; Cell_synth_C. Pfam:PF05420; BCSC_C; 1. ant_ant_sig: anti-anti-sigma factor No signal peptide present SignalP predicted). No transmembrane helices present (TMHMM predicted); Function unclear; hypothetical protein 447230 4606394 azo0422 Azoarcus sp. BH72 hypothetical protein YP_931926.1 445887 D 62928 CDS YP_931927.1 119896714 4606395 447262..447798 1 NC_008702.1 heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease peptidase subunit 447798 hslV 4606395 hslV Azoarcus sp. BH72 ATP-dependent protease peptidase subunit YP_931927.1 447262 D 62928 CDS YP_931928.1 119896715 4609037 447833..449164 1 NC_008702.1 heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease ATP-binding subunit HslU 449164 hslU 4609037 hslU Azoarcus sp. BH72 ATP-dependent protease ATP-binding subunit HslU YP_931928.1 447833 D 62928 CDS YP_931929.1 119896716 4609036 449266..450138 1 NC_008702.1 Putative malonate transporter. subcellular location:integral membrane protein (potential). similarity: belongs to the auxin efflux carrier (tc 2.a.69) family. SPROT:P56948:24% identity, 41% similarity. InterPro: IPR004776; Auxin_eff. Pfam:PF03547; Auxin_eff 2a69: Auxin Efflux Carrier; Specificity unclear; putative malonate transporter 450138 mdcF1 4609036 mdcF1 Azoarcus sp. BH72 putative malonate transporter YP_931929.1 449266 D 62928 CDS YP_931930.1 119896717 4609201 complement(450142..452085) 1 NC_008702.1 GGDEF/PAS-domain containing protein; diguanylate cyclase 452085 4609201 azo0426 Azoarcus sp. BH72 diguanylate cyclase YP_931930.1 450142 R 62928 CDS YP_931931.1 119896718 4606396 complement(452338..452742) 1 NC_008702.1 Heat shock protein 15 homolog (HSP15). INVOLVED IN THE RECYCLING OF FREE 50S RIBOSOMAL SUBUNITS THAT STILL CARRY A NASCENT CHAIN. BINDS RNA MORE SPECIFICALLY THAN DNA. BINDS WITH VERY HIGH AFFINITY TO THE FREE 50S RIBOSOMAL SUBUNIT. DOES NOT BIND IT WHEN IT IS PART OF THE 70S RIBOSOME; Conserved hypothetical protein; heat shock protein 452742 hslR 4606396 hslR Azoarcus sp. BH72 heat shock protein YP_931931.1 452338 R 62928 CDS YP_931932.1 119896719 4609035 complement(452792..454072) 1 NC_008702.1 55% Adenylylsulfate kinase (EC 2.7.1.25) (APS kinase) (ATP adenosine-5-phosphosulfate 3-phosphotransferase)], from Pseudomonas aeruginosa. FUNCTION:ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity (By similarity). FUNCTION:APS kinase catalyzes the synthesis of activated sulfate (By similarity). CATALYTIC ACTIVITY:ATP + sulfate = diphosphate + adenylylsulfate. CATALYTIC ACTIVITY:ATP + adenylylsulfate=ADP +3'-phosphoadenylylsulfate. PATHWAY: Sulfate activation; cysteine biosynthesis reductive branch; first step. PATHWAY:Sulfate activation; cysteine biosynthesis reductive branch; second step. SUBUNIT:Heterodimer composed of cysD, the smaller subunit,and cysN (By similarity). SIMILARITY:In the N-terminal section; belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily. SIMILARITY:In the C-terminal section; Belongs to the APS kinase family. SWISSPROT:CYSN_PSEAE:O50274. InterPro:IPR002891;APS_kinase.IPR004161;EFTU_D2.IPR009001; Elong_init_C.IPR000795; ProtSyn_GTPbind.IPR005225; Small_GTP.IPR009000; Translat_factor. Pfam:PF01583; APS_kinase; 1.PF00009; GTP_EFTU; 1.PF03144; GTP_EFTU_D2;1. TIGRFAMs:TIGR00455; apsK; 1.TIGR00231; small_GTP; 1.; High confidence in function and specificity; putative sulfate adenylyltransferase subunit 1/adenylylsulfate kinase 454072 cysN/C 4609035 cysN/C Azoarcus sp. BH72 putative sulfate adenylyltransferase subunit 1/adenylylsulfate kinase YP_931932.1 452792 R 62928 CDS YP_931933.1 119896720 4608562 complement(454492..455406) 1 NC_008702.1 with CysN catalyzes the formation of adenylylsulfate from sulfate and ATP; sulfate adenylyltransferase subunit 2 455406 cysD 4608562 cysD Azoarcus sp. BH72 sulfate adenylyltransferase subunit 2 YP_931933.1 454492 R 62928 CDS YP_931934.1 119896721 4608556 complement(455534..456304) 1 NC_008702.1 catalyzes the reduction of 3'-phosphoadenylyl sulfate into sulfite; phosphoadenosine phosphosulfate reductase 456304 cysH 4608556 cysH Azoarcus sp. BH72 phosphoadenosine phosphosulfate reductase YP_931934.1 455534 R 62928 CDS YP_931935.1 119896722 4608558 complement(456301..456831) 1 NC_008702.1 Conserved hypothetical protein. Homology to PA1837 of P.aeruginosa of 50% (tremble:Q9I2Q8). Has PF06073,Bacterial protein of unknown function (DUF934);IPR008318 UCP030820: This family consists of several bacterial proteins of unknown function. One of the members of this family Q8YEW3 is thought to be an oxidoreductase. No signal peptide. No TMHs; hypothetical protein 456831 4608558 azo0431 Azoarcus sp. BH72 hypothetical protein YP_931935.1 456301 R 62928 CDS YP_931936.1 119896723 4606397 complement(456824..458506) 1 NC_008702.1 49% Fd-NiR.IPR011255; NiR_SiRalpha_1/3.IPR006067; Nir_Sir_4Fe4S.IPR005117; NiR_SiR_beta_fer. Pfam:PF01077; NIR_SIR; 2.PF03460; NIR_SIR_ferr; 2.; High confidence in function and specificity; putative sulfite reductase 458506 cysI 4606397 cysI Azoarcus sp. BH72 putative sulfite reductase YP_931936.1 456824 R 62928 CDS YP_931937.1 119896724 4608559 complement(458559..459338) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to bll1489 of B. japonicum of 58% (trembl|Q89UC8(SRS)) InterPro:IPR002781; DUF81. Pfam:PF01925; DUF81 Presence of 8 transmembrane helices (TMHMM predicted) probable transmembrane protein of unknown function) no signal peptide; Conserved hypothetical protein; hypothetical protein 459338 4608559 azo0433 Azoarcus sp. BH72 hypothetical protein YP_931937.1 458559 R 62928 CDS YP_931938.1 119896725 4606398 459444..460385 1 NC_008702.1 HTH-type transcriptional regulator cysB (Cys regulon transcriptional activator). this protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of l-cysteine from inorganic sulfate. the inducer for cysb is n-acetylserine. cysb inhibits its own transcription.; High confidence in function and specificity; transcriptional regulator CysB-like protein 460385 cysB 4606398 cysB Azoarcus sp. BH72 transcriptional regulator CysB-like protein YP_931938.1 459444 D 62928 CDS YP_931939.1 119896726 4608555 460530..461414 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. Signal peptide present. No TMHs; hypothetical protein 461414 4608555 azo0435 Azoarcus sp. BH72 hypothetical protein YP_931939.1 460530 D 62928 CDS YP_931940.1 119896727 4606399 complement(461424..462398) 1 NC_008702.1 L-asparaginase precursor (EC 3.5.1.1) (L-asparagine amidohydrolase) (L-ASNase).; Family membership; L-asparaginase II 462398 4606399 azo0436 Azoarcus sp. BH72 L-asparaginase II YP_931940.1 461424 R 62928 CDS YP_931941.1 119896728 4606400 complement(462427..463827) 1 NC_008702.1 Region start changed from 463959 to 463827 (-132 bases); aspartate ammonia-lyase 463827 4606400 azo0437 Azoarcus sp. BH72 aspartate ammonia-lyase YP_931941.1 462427 R 62928 CDS YP_931942.1 119896729 4606401 complement(463964..465061) 1 NC_008702.1 L-asparaginase precursor (EC 3.5.1.1) (L-asparagine amidohydrolase) (L-ASNase).; High confidence in function and specificity; periplasmic L-asparaginase II 465061 ansB2 4606401 ansB2 Azoarcus sp. BH72 periplasmic L-asparaginase II YP_931942.1 463964 R 62928 CDS YP_931943.1 119896730 4608321 complement(465187..465915) 1 NC_008702.1 Glutamine transport ATP-binding protein glnQ. Homology with glnQ of B. stearothermophilus of 56% (sprot|GLNQ_BACST). PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR GLUTAMINE. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. InterPro: AAA ATPase superfamily (IPR003593), ABC_transporter (IPR003439), ATP/GTP_binding site motif A (P-loop) (IPR001687) Pfam: ABC transporter no signal peptide no TMHs; High confidence in function and specificity; glutamine transport ATP-binding protein 465915 glnQ1 4608321 glnQ1 Azoarcus sp. BH72 glutamine transport ATP-binding protein YP_931943.1 465187 R 62928 CDS YP_931944.1 119896731 4608902 complement(465947..466627) 1 NC_008702.1 Putative glutamine transport permease. Homology to glnM of B. subtilis of 30% (TREMBL:O34671) Probably part of the binding-protein-dependent transport system of amino acids. Probably responsible for the translocation of the substrate across the membrane InterPro: Binding-protein-dependent transport systems inner membrane component (IPR000515) Pfam: Binding-protein-dependent transport systems inner membrane component no signal peptide probable 5 TMHs; Family membership; putative glutamine transport permease 466627 glnM 4608902 glnM Azoarcus sp. BH72 putative glutamine transport permease YP_931944.1 465947 R 62928 CDS YP_931945.1 119896732 4608900 complement(466629..467366) 1 NC_008702.1 Glutamate/aspartate transport system permease gltJ. Homology to glnP of B. subtilis of 23% (CAA93320). PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR AMINO ACID; PROBABLY RESPONSIBLE FOR THE TRANSLOCATION OF THE SUBSTRATE ACROSS THE MEMBRANE. InterPro: Binding-protein-dependent transport systems inner membrane component (IPR000515) Pfam: Binding-protein-dependent transport systems inner membrane component HTH-motif probable 4 TMHs no signal peptide; Family membership; putative glutamine transport permease 467366 glnP 4608900 glnP Azoarcus sp. BH72 putative glutamine transport permease YP_931945.1 466629 R 62928 CDS YP_931946.1 119896733 4608901 complement(467763..468662) 1 NC_008702.1 Putative glutamine-binding protein. Homology to glnH of B. stearothermophilus of 28% (sprot|GLNH_BACST). Involved in glutamine-transport system. Interacts with the glutamine-transport system glnPQM. Pfam: Bacterial extracellular solute binding protein no TMHs signal peptide; Family membership; putative glutamine-binding protein 468662 glnH 4608901 glnH Azoarcus sp. BH72 putative glutamine-binding protein YP_931946.1 467763 R 62928 CDS YP_931947.1 119896734 4608898 468959..470002 1 NC_008702.1 Conserved Hypothetical protein(Metallo-beta-lactamase superfamily). Has PF00753, Metallo-beta-lactamase superfamily; IPR001279, Blactmase-like;These proteins include thiolesterases, members of the glyoxalase II family, that catalyse the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid and a competence protein that is essential for natural transformation in Neisseria gonorrhoeae and could be a transporter involved in DNA uptake. Except for the competence protein these proteins bind two zinc ions per molecule as cofactor.; Family membership; beta-lactamase 470002 4608898 azo0443 Azoarcus sp. BH72 beta-lactamase YP_931947.1 468959 D 62928 CDS YP_931948.1 119896735 4606402 469999..471642 1 NC_008702.1 catalyzes the ATP-dependent formation of a phosphodiester at the site of a single strand break in duplex DNA; ATP-dependent DNA ligase 471642 lig2 4606402 lig2 Azoarcus sp. BH72 ATP-dependent DNA ligase YP_931948.1 469999 D 62928 CDS YP_931949.1 119896736 4609138 471695..474442 1 NC_008702.1 Putative ATP-dependent helicase MTH1802. TREMBL:Q88NV1: 57% identity, 66% similarity InterPro:IPR001410; DEAD/DEAH box helicase IPR001650; Helicase_C. Pfam: PF00270; DEAD; 1. PF00271; Helicase_C; 1. SMART: SM00487; DEXDc; 1. SM00490: HELICc livcs: branched-chain amino acid trans Non secretory protein with low probablity of cleavage site (0.167): Signal P predicted. No transmembrane helices (TMHMM predicted); High confidence in function and specificity; ATP-dependent helicase 474442 4609138 azo0445 Azoarcus sp. BH72 ATP-dependent helicase YP_931949.1 471695 D 62928 CDS YP_931950.1 119896737 4606403 474445..475113 1 NC_008702.1 Probable Hypothetical protein MJ0037. Members of this uncharacterized family share a motif approximating DXH(X25)GDXXD(X25)GNHD as found in several phosphoesterases, including the nucleases SbcD and Mre11. SbcD is a subunit of the SbcCD nuclease of Escherichia coli that can cleave DNA hairpins to unblock stalled DNA replication. All members of this family are archaeal TREMBL:Q88NV2: 46% identity, 59% similarity InterPro:IPR004843; M-ppestrase. Pfam: PF00149; Metallophos; No transmembrane helices TIGR00024: conserved hypothetical prot; Function unclear; hypothetical protein 475113 4606403 azo0446 Azoarcus sp. BH72 hypothetical protein YP_931950.1 474445 D 62928 CDS YP_931951.1 119896738 4606404 complement(475122..476456) 1 NC_008702.1 C4-dicarboxylate transport transcriptional regulatory protein,; High confidence in function and specificity; transcriptional regulator 476456 dctD1 4606404 dctD1 Azoarcus sp. BH72 transcriptional regulator YP_931951.1 475122 R 62928 CDS YP_931952.1 119896739 4608595 complement(476453..478414) 1 NC_008702.1 C4-dicarboxylate transport sensor protein,; High confidence in function and specificity; sensor histidine kinase 478414 dctB1 4608595 dctB1 Azoarcus sp. BH72 sensor histidine kinase YP_931952.1 476453 R 62928 CDS YP_931953.1 119896740 4608593 complement(478380..480146) 1 NC_008702.1 Glutathione-regulated potassium-efflux system protein kefB,(K+/H+ antiporter).Transport system that facilitates potassium-efflux possibly by potassium-proton antiport. 32% K_eff.IPR006153; Na_H_porter.IPR006036;TrkA_Kuptake. IPR003148; TrkA_N. Pfam:PF00999; Na_H_Exchanger; 1.PF02254; TrkA-N; 1. TIGRFAMs:TIGR00932; 2a37; 1. TMHelix:12. Belongs to the monovalent cation:proton antiporter 2 (cpa2) transporter (TC 2.A.37) family. KefB subfamily.; High confidence in function and specificity; putative glutathione-regulated potassium-efflux system protein 480146 kefB 4608593 kefB Azoarcus sp. BH72 putative glutathione-regulated potassium-efflux system protein YP_931953.1 478380 R 62928 CDS YP_931954.1 119896741 4609105 480291..482036 1 NC_008702.1 PAS/PAC/GGDEF-domain containing protein,implicating involvement into signalling processes. Similarity to TREMBL: trembl|Q98JA6 (30% Rhizobium loti,hypothetical protein mlr2027) InterPro: IPR000160 GGDEF. IPR001610 PAC. IPR000700 PAS-assoc_C. IPR000014 PAS_domain. Pfam: PF00990 GGDEF. SMART: SM00086 PAC. SM00091 PAS. TIGRFAM: TIGR00254 GGDEF. TIGR00229 sensory_box.; PAS/PAC/GGDEF-domain-containing protein 482036 4609105 azo0450 Azoarcus sp. BH72 PAS/PAC/GGDEF-domain-containing protein YP_931954.1 480291 D 62928 CDS YP_931955.1 119896742 4606405 complement(482053..482613) 1 NC_008702.1 Hypothetical secreted protein. No homology with hits in the Database. No domains predicted. Signal peptide present. No TMHs; hypothetical protein 482613 4606405 azo0451 Azoarcus sp. BH72 hypothetical protein YP_931955.1 482053 R 62928 CDS YP_931956.1 119896743 4606406 complement(482675..483571) 1 NC_008702.1 Putative thioredoxin reductase. Homology to trxB of S. coelicolor of 25% (sprot|TRXB_STRCO). Catalyse the reaction: thioredoxin + nadp(+) = thioredoxin disulfide + nadph. InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327) Pfam: Pyridine nucleotide-disulphide oxidoreductase no signal peptide no TMHs; Family membership; putative thioredoxin-disulfide reductase 483571 trxB1 4606406 trxB1 Azoarcus sp. BH72 putative thioredoxin-disulfide reductase YP_931956.1 482675 R 62928 CDS YP_931957.1 119896744 4610041 complement(483653..485464) 1 NC_008702.1 Probable composite transport ATP-binding permease protein. Homology to amt1 of S. cereviciae of 48% (sprot|ATM1_YEAST). COULD BE INVOLVED IN THE TRANSPORT OF yet unknow substrates (probable HEME) FROM THE MITOCHONDRIA TO THE CYTOSOL. InterPro: ABC transporter transmembrane region (IPR001140), ATP/GTP-binding site motif A (P loop) (IPR001687), ABC transporter (IPR003439),AAA_ATPase sperfamily (IPR003593) Pfam: ATP transporterABC transporter transmembrane region no signal peptide probable 6 TMHs; Specificity unclear; composite transport ATP-binding permase 485464 atm1 4610041 atm1 Azoarcus sp. BH72 composite transport ATP-binding permase YP_931957.1 483653 R 62928 CDS YP_931958.1 119896745 4608364 complement(485487..486122) 1 NC_008702.1 Conserved hypothetical threonine efflux protein. Homology to rsc0148 of R. solanaxearum of 52% (trembl|Q8Y2B8). InterPro: Lysine exporter protein (LYSE/YGGA) (IPR001123) Pfam: LysE type translocator Tigrfam: 2A76: Homoserine/Threonine efflux protein signal peptide Probable 6 TMHs; Family membership; putative threonine efflux protein 486122 4608364 azo0454 Azoarcus sp. BH72 putative threonine efflux protein YP_931958.1 485487 R 62928 CDS YP_931959.1 119896746 4606407 486445..487725 1 NC_008702.1 This family of proteins contains P47K, a Pseudomonas chlororaphis protein needed for nitrile hydratase expression, and the cobW gene product, which may be involved in cobalamin biosynthesis in Pseudomonas denitrificans [1]. TREMBL:Q8YJP6: 40% identity, 54% similarity. Pfam:PF02492; Cobalmine synthesis protein; pfkB family carbohydrate kinase; FAD binding domain. TIGRFAM:proC: pyrroline 5 carboxylate reductase. mobB: molybdopterin-guanine dinucleotide; Family membership; putative cobalmin snthesis protein 487725 4606407 azo0455 Azoarcus sp. BH72 putative cobalmin snthesis protein YP_931959.1 486445 D 62928 CDS YP_931960.1 119896747 4606408 487845..488045 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. signal peptide. no TMHs; hypothetical protein 488045 4606408 azo0456 Azoarcus sp. BH72 hypothetical protein YP_931960.1 487845 D 62928 CDS YP_931961.1 119896748 4606409 488198..488884 1 NC_008702.1 Response regulator,; High confidence in function and specificity; two-component response regulator 488884 ragA 4606409 ragA Azoarcus sp. BH72 two-component response regulator YP_931961.1 488198 D 62928 CDS YP_931962.1 119896749 4609693 488874..490262 1 NC_008702.1 Putative sensor kinase,; High confidence in function and specificity; putative two component sensor kinase 490262 ragB 4609693 ragB Azoarcus sp. BH72 putative two component sensor kinase YP_931962.1 488874 D 62928 CDS YP_931963.1 119896750 4609694 490448..491650 1 NC_008702.1 Putative ammonium transporter MJ1343. A number of evolutionarily-related proteins have been found to be involved in the transport of ammonium ions across membranes.Represent a family of high affinity ammonium transporters. These proteins are highly hydrophobic and seem to contain from 10 to 12 transmembrane domain. TREMBL:Q88L29: 73% identity, 84% similarity InterPro: Ammonium transporter family InterPro: IPR001905; Ammonium_transpt. IPR010256; RH_like_transpt. Pfam: PF00909; Ammonium_transp; 1. PROSITE: PS01219; AMMONIUM_TRANSP amt: ammonium transporter Non secretory protein with signal peptide probability of 0.174 (SignalP predicted) TMHMM predicted 11 TMH's; High confidence in function and specificity; ammonia permease 491650 amtD 4609694 amtD Azoarcus sp. BH72 ammonia permease YP_931963.1 490448 D 62928 CDS YP_931964.1 119896751 4608317 complement(491666..492280) 1 NC_008702.1 Putative Dnr-like transcriptional activator. Similar to SWISSPROT: sprot|FIXK_RHIME (28% Rhizobium meliloti, FixK) InterPro: IPR000595 cNMP_binding. IPR001808 HTH_Crp. Pfam: PF00027 cNMP_binding. HTH reporting nucleic acid binding motif.; Family membership; putative Dnr-like transcriptional activator 492280 dnr 4608317 dnr Azoarcus sp. BH72 putative Dnr-like transcriptional activator YP_931964.1 491666 R 62928 CDS YP_931965.1 119896752 4608641 492369..492854 1 NC_008702.1 Hypthetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Has signal peptide.; hypothetical protein 492854 4608641 azo0461 Azoarcus sp. BH72 hypothetical protein YP_931965.1 492369 D 62928 CDS YP_931966.1 119896753 4606410 complement(492897..495704) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to an orf of Trichodesmium erythraeum of 45% (gi|48892210|ref|ZP_00325608.1|(NBCI ENTREZ)). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 495704 4606410 azo0462 Azoarcus sp. BH72 hypothetical protein YP_931966.1 492897 R 62928 CDS YP_931967.1 119896754 4606411 495962..498433 1 NC_008702.1 functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; acyl-CoA dehydrogenase 498433 fadE 4606411 fadE Azoarcus sp. BH72 acyl-CoA dehydrogenase YP_931967.1 495962 D 62928 CDS YP_931968.1 119896755 4608726 498430..499902 1 NC_008702.1 Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 499902 fadA1 4608726 fadA1 Azoarcus sp. BH72 acetyl-CoA acetyltransferase YP_931968.1 498430 D 62928 CDS YP_931969.1 119896756 4608717 499899..501935 1 NC_008702.1 alpha-subunit of fatty acid oxidation complex. Entry name TREMBL:Q8G968 Prim. accession # Q8G968 Identities = 373/642 (58%) InterPro IPR006108; 3HCDH_C. IPR006176; 3HCDH_N. Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0 IPR008927; 6DGDH_C_like. IPR001753; EnCoA_hydrtse. Pfam PF00725; 3HCDH; 1. PF02737; 3HCDH_N; 1. PF00378; ECH; 1.; Family membership; enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase 501935 fadB1 4608717 fadB1 Azoarcus sp. BH72 enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase YP_931969.1 499899 D 62928 CDS YP_931970.1 119896757 4608720 501932..502336 1 NC_008702.1 Conserved hypothetical acyl-CoA thioesterase. Homology to an orf of Azoarcus sp. EbN1 (trembl|Q8G9D6). Pfam: cytosolic long-chain acyl-CoA thioesterase Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyse the hydrolysis of other long chain fatty acyl-CoA thioesters. Interpro: Thioesterase superfamily (IPR006683) no signal peptide no. TMHs; Family membership; acyl-CoA thioesterase 502336 4608720 azo0466 Azoarcus sp. BH72 acyl-CoA thioesterase YP_931970.1 501932 D 62928 CDS YP_931971.1 119896758 4606412 502438..503721 1 NC_008702.1 Long-chain fatty acid transport protein precursor (Outer membrane fadL protein) (Outer membrane flp protein). Involved in translocation of long-chain fatty acids across the outer membrane. FadL may form a specific channel (By similarity). Entry name:- Q8G9D7 Primary accession number:- Q8G9D7 InterPro:- IPR005017; Toluene_X. Pfam:- PF03349; Toluene_X; 1. identity:- 66% Prediction: Signal peptide Signal peptide probability: 1.0 Number of predicted TMHs: 0; High confidence in function and specificity; long-chain fatty acid ABC transporter 503721 fadL 4606412 fadL Azoarcus sp. BH72 long-chain fatty acid ABC transporter YP_931971.1 502438 D 62928 CDS YP_931972.1 119896759 4608728 503770..506154 1 NC_008702.1 Short-chain enoyl-CoA hydratase activity . Activity:- 3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O Entry name TREMBL:Q8P986 Prim. accession # Q8P986 InterPro IPR006108; 3HCDH_C. IPR006176; 3HCDH_N. IPR008927; 6DGDH_C_like. IPR001753; EnCoA_hydrtse. IPR000205; NAD_BS. Pfam PF00725; 3HCDH; 1. PF02737; 3HCDH_N; 1. PF00378; ECH; 1. Identities = 468/796 (58%) Prediction: Signal peptide Signal peptide probability: 0.980 Number of predicted TMHs: 0; Family membership; 3-hydroxyacyl-CoA dehydrogenase 506154 fadB2 4608728 fadB2 Azoarcus sp. BH72 3-hydroxyacyl-CoA dehydrogenase YP_931972.1 503770 D 62928 CDS YP_931973.1 119896760 4608721 506636..507868 1 NC_008702.1 Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 507868 fadA2 4608721 fadA2 Azoarcus sp. BH72 acetyl-CoA acetyltransferase YP_931973.1 506636 D 62928 CDS YP_931974.1 119896761 4608718 507945..508745 1 NC_008702.1 Function:- COULD POSSIBLY OXIDIZES FATTY ACIDS USING SPECIFIC COMPONENTS (BY SIMILARITY). CATALYTIC ACTIVITY:-(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O. Entry name SWISSPROT:PAAG_ECOLI Prim. accession # P77467 InterPro:- IPR001753; EnCoA_hydrtse. Pfam:-PF00378; ECH; 1. Identities = 92/265 (34%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; enoyl-CoA hydratase 508745 paaG2 4608718 paaG2 Azoarcus sp. BH72 enoyl-CoA hydratase YP_931974.1 507945 D 62928 CDS YP_931975.1 119896762 4609473 complement(508774..509625) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv1508 of C. violaceum of 37% (trembl|Q7NXW8). Pfam: uncharacterized ACR, COG1434. no signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 509625 4609473 azo0471 Azoarcus sp. BH72 hypothetical protein YP_931975.1 508774 R 62928 CDS YP_931976.1 119896763 4606413 509624..510913 1 NC_008702.1 Probable O-acetylhomoserine aminocarboxypropyltransferase. Homology to cysD of A. nidulans of 54% (sprot|CYSD_EMENI). Transforms O-acetylhomoserine into homocysteine. InterPro: Cys/Met metabolism pyridoxal-phosphate-dependent enzymes (IPR000277) Pfam: Cys/Met metabolism PLP-dependent enzymes no signal peptide no TMHs; High confidence in function and specificity; O-acetylhomoserine aminocarboxypropyltransferase 510913 4606413 azo0472 Azoarcus sp. BH72 O-acetylhomoserine aminocarboxypropyltransferase YP_931976.1 509624 D 62928 CDS YP_931977.1 119896764 4606414 510910..511497 1 NC_008702.1 Hypothetical membrane protein. No good homology to the data bank. No domains predicted. 1 TMHs No signal peptide present.; hypothetical protein 511497 4606414 azo0473 Azoarcus sp. BH72 hypothetical protein YP_931977.1 510910 D 62928 CDS YP_931978.1 119896765 4606415 511494..512357 1 NC_008702.1 Exported protein with sporulation related repeat. Pfam: Sporulation related repeat. InterPro: Proline-rich region; Function unclear; peptidoglycan-binding protein 512357 4606415 azo0474 Azoarcus sp. BH72 peptidoglycan-binding protein YP_931978.1 511494 D 62928 CDS YP_931979.1 119896766 4606416 512441..512881 1 NC_008702.1 binds to flagellin and appears to stabilize flagellin during flagella assembly; flagellar assembly protein FliW 512881 4606416 azo0475 Azoarcus sp. BH72 flagellar assembly protein FliW YP_931979.1 512441 D 62928 CDS YP_931980.1 119896767 4606417 512981..513361 1 NC_008702.1 Conserved hypothetical endoribonuclase L-PSP. Homology to orf1 of A. vineladii of 74% (sprot|YVN1_AZOVI(SRS)) This protein was described initially as an inhibitor of protein synthesis intiation but is now viewed as an endoribonuclease active on single-stranded mRNA. The cleavage of mRNA is responsible for the inhibition of protein synthesis. A role in purine regulation has also been suggested. Tigrfam: Endoribonuclease L-PSP, putative Pfam: Endoribonuclease L-PSP (=YipgF family) no TMHs weak indication for signal peptide; Conserved hypothetical protein; endoribonuclease L-PSP 513361 4606417 azo0476 Azoarcus sp. BH72 endoribonuclease L-PSP YP_931980.1 512981 D 62928 CDS YP_931981.1 119896768 4606418 513369..515408 1 NC_008702.1 catalyzes branch migration in Holliday junction intermediates; ATP-dependent DNA helicase RecG 515408 recG 4606418 recG Azoarcus sp. BH72 ATP-dependent DNA helicase RecG YP_931981.1 513369 D 62928 CDS YP_931982.1 119896769 4609706 515463..516032 1 NC_008702.1 Chorismate:pyruvate lyase (EC 4.-.-.-). catalytic activity: chorismate = 4-hydroxybenzoate + pyruvate. pathway: ubiquinone biosynthesis; first step.; Specificity unclear; putative chorismate:pyruvate lyase 516032 ubiC 4609706 ubiC Azoarcus sp. BH72 putative chorismate:pyruvate lyase YP_931982.1 515463 D 62928 CDS YP_931983.1 119896770 4610057 516019..516882 1 NC_008702.1 catalyzes the conversion of 4-Hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate, as part of the ubiquinone biosynthesis pathway; 4-hydroxybenzoate octaprenyltransferase 516882 ubiA 4610057 ubiA Azoarcus sp. BH72 4-hydroxybenzoate octaprenyltransferase YP_931983.1 516019 D 62928 CDS YP_931984.1 119896771 4610056 complement(516980..520849) 1 NC_008702.1 Conserved hypothetical irons-sulfur binding oxidase. Homology to cv4235 of C. violaceum of 70% (trembl|Q7NQA5) Pfam: FAD binding domain; FAD linked oxidase, Cterminal domain Tigrfam: glcD: glycolate oxidase subunit GlcD no signal peptide no TMHs; Family membership; iron-sulfur binding oxidase 520849 4610056 azo0480 Azoarcus sp. BH72 iron-sulfur binding oxidase YP_931984.1 516980 R 62928 CDS YP_931985.1 119896772 4606419 complement(520972..521325) 1 NC_008702.1 hypothetical protein 521325 4606419 azo0481 Azoarcus sp. BH72 hypothetical protein YP_931985.1 520972 R 62928 CDS YP_931986.1 119896773 4606420 complement(521370..521783) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to yqaA of Nitrosomonas europaea of 50% (trembl|Q82TH7(SRS)) No domains predicted. Signal peptide present. TMHMM2 reporting presence of 2 TMH's.; Conserved hypothetical protein; hypothetical protein 521783 4606420 azo0482 Azoarcus sp. BH72 hypothetical protein YP_931986.1 521370 R 62928 CDS YP_931987.1 119896774 4606421 521919..522353 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA4874 of P.aeruginosa of 36% (trembl|Q9HUT9(SRS)) No domains predicted. Signal petide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 522353 4606421 azo0483 Azoarcus sp. BH72 hypothetical protein YP_931987.1 521919 D 62928 CDS YP_931988.1 119896775 4606422 complement(522433..523299) 1 NC_008702.1 FHA domain containing protein. Best similarity to SWISSPROT: sprot|REPB_AGRRH (9% Agrobacterium rhizogenes,possible replication protein b) Pfam: PF00211 Adenylate and Guanylate cyclase catalytic domain. SMART: SM00240 FHA Forkhead associated domain.; FHA domain-containing protein 523299 4606422 azo0484 Azoarcus sp. BH72 FHA domain-containing protein YP_931988.1 522433 R 62928 CDS YP_931989.1 119896776 4606423 complement(523396..524316) 1 NC_008702.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 524316 4606423 azo0485 Azoarcus sp. BH72 C32 tRNA thiolase YP_931989.1 523396 R 62928 CDS YP_931990.1 119896777 4606424 complement(524389..525135) 1 NC_008702.1 Involved in pteridine salvage and antifolate resistance; pteridine reductase 525135 4606424 azo0486 Azoarcus sp. BH72 pteridine reductase YP_931990.1 524389 R 62928 CDS YP_931991.1 119896778 4606425 525247..526419 1 NC_008702.1 Conserved hypothetical protein. Homology to NE1166 of N.europaea of 49% (tremble:Q82VC8) Has PF02636,Uncharacterized ACR, COG1565;IPR003788 ,DUF185; This family contains several uncharacterised proteins. Q8YI87 has been described as an ATP synthase beta subunit transcription termination factor rho protein. No signal peptide. No TMHs.; hypothetical protein 526419 4606425 azo0487 Azoarcus sp. BH72 hypothetical protein YP_931991.1 525247 D 62928 CDS YP_931992.1 119896779 4606426 complement(526459..526677) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3372 of Azoarcus sp. EbN1 of 47% (gnl|keqq|eba:ebA3372(KEGG)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 526677 4606426 azo0488 Azoarcus sp. BH72 hypothetical protein YP_931992.1 526459 R 62928 CDS YP_931993.1 119896780 4606427 526664..526792 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 526792 4606427 azo0489 Azoarcus sp. BH72 hypothetical protein YP_931993.1 526664 D 62928 CDS YP_931994.1 119896781 4606428 complement(526887..527693) 1 NC_008702.1 TREMBL:Q8F3A6: 43% identity, 60% similarity Non-heme chloroperoxidase (EC 1.11.1.10) (Chloride peroxidase) (CPO-P) (Chloroperoxidase P). CHLORINATES AND BROMINATES SUITABLE ORGANIC COMPOUNDS. INVOLVED IN THE BIOSYNTHESIS OF THE ANTIBIOTIC PYRROLNITRIN. InterPro: Alpha/beta hydrolase fold InterPro:IPR000073; A/b_hydrolase. IPR003089: AB_hydrolase. IPR000379: Ser_estrs. Pfam:PF00561; Abhydrolase_1; 1 TIGRFAM:dsbE: periplasmic protein thiol:disulf; Family membership; putative acetone-cyanohydrin lyase 527693 4606428 azo0490 Azoarcus sp. BH72 putative acetone-cyanohydrin lyase YP_931994.1 526887 R 62928 CDS YP_931995.1 119896782 4606429 complement(527690..528310) 1 NC_008702.1 Probable glutathione S-transferase. Homology to isoJ of Rhodococcus sp AD45 of 48% (trembl|Q9RBP3). Catalysis of the reaction: R-X + glutathione = H-X + R-S-glutathione. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Pfam: Glutathione S-transferase, N-terminal domain; Glutathione S-transferase, C-terminal domain no signal peptide no TMHs; High confidence in function and specificity; glutathione S-transferase 528310 isoJ 4606429 isoJ Azoarcus sp. BH72 glutathione S-transferase YP_931995.1 527690 R 62928 CDS YP_931996.1 119896783 4609093 528392..528589 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BH1226 of Bacillus halodurans of 40% (pir|B83803) no domains predicted. signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 528589 4609093 azo0492 Azoarcus sp. BH72 hypothetical protein YP_931996.1 528392 D 62928 CDS YP_931997.1 119896784 4606430 complement(528605..529843) 1 NC_008702.1 catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template; phosphohydrolase activities include hydrolysis of pyrophosphate, 5'-nucleoside tri- and diphosphates, NADP, and 2'-AMP with the production of Pi, metal-dependent phosphodiesterase activity for 2',3'-cAMP, 2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic substrates with the formation of 2'-nucleotides and 3'-nucleotides; these phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases; multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase 529843 cca 4606430 cca Azoarcus sp. BH72 multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase YP_931997.1 528605 R 62928 CDS YP_931998.1 119896785 4608441 complement(529843..530487) 1 NC_008702.1 Putative stringent starvation protein A. Homology to sspA of E. coli of 26% (sprot|SSPA_ECOLI). FORMS AN EQUIMOLAR COMPLEX WITH THE RNA POLYMERASE HOLOENZYME (RNAP) BUT NOT WITH THE CORE ENZYME. IT IS SYNTHESIZED PREDOMINANTLY WHEN CELLS ARE EXPOSED TO AMINO ACID STARVATION AT WHICH TIME IT ACCOUNTS FOR OVER 50% OF THE TOTAL PROTEIN SYNTHESIZED. InterPro: Glutathione S-transferase N terminus (IPR001045) Pfam: Glutathione S-transferase, N-terminal domaine no signal peptide no TMHs; Family membership; putative stringent starvation protein A 530487 sspA1 4608441 sspA1 Azoarcus sp. BH72 putative stringent starvation protein A YP_931998.1 529843 R 62928 CDS YP_931999.1 119896786 4609895 complement(530568..531536) 1 NC_008702.1 33%; Specificity unclear; putative NADH dehydrogenase 531536 4609895 azo0495 Azoarcus sp. BH72 putative NADH dehydrogenase YP_931999.1 530568 R 62928 CDS YP_932000.1 119896787 4606431 complement(531552..533480) 1 NC_008702.1 Putative soluble lytic murein transglycosylase. Homology to slt of E. coli of 24% (sprot|SLT_ECOLI). Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division. InterPro: SLT domain (IPR000189) Pfam: Transglycosylase SLT domain singal peptide no TMHs; High confidence in function and specificity; putative soulbe lytic murein transglycosylase 533480 sltY 4606431 sltY Azoarcus sp. BH72 putative soulbe lytic murein transglycosylase YP_932000.1 531552 R 62928 CDS YP_932001.1 119896788 4609874 533521..534114 1 NC_008702.1 5-formyltetrahydrofolate cyclo-ligase InterPro: 5-formyltetrahydrofolate cyclo-ligase; Specificity unclear; hypothetical protein 534114 4609874 azo0497 Azoarcus sp. BH72 hypothetical protein YP_932001.1 533521 D 62928 CDS YP_932002.1 119896789 4606432 complement(534121..536826) 1 NC_008702.1 GGDEF/PAS/PAC-domain containing protein. Similarity to SWISSPROT: sprot|Y4LL_RHISN (13% Rhizobium sp. (strain NGR234), hypothetical 91.8 kda protein y4ll) / TREMBL: trembl|Q55955 (14% Synechocystis sp. (strain PCC 6803),sll0779) Pfam: PF00990 GGDEF domain. PF00989 PAS. PF00785 PAC. TIGRFAM: TIGR00254 putative diguanylate cyclase (GGDEF) domain. TIGR00229 PAS domain S-box. TMHMM reporting 2 transmembrane helices.; diguanylate cyclase 536826 4606432 azo0498 Azoarcus sp. BH72 diguanylate cyclase YP_932002.1 534121 R 62928 CDS YP_932003.1 119896790 4606433 complement(536846..537652) 1 NC_008702.1 Putative cyclohexadienyl dehydratase precursor. Homology to pheC of P. aeruginosa of 36% (sprot|PHEC_PSEAE) FORMS ALTERNATIVE PATHWAY FOR PHENYLALANINE BIOSYNTHESIS. CAN CATALYZE TWO REACTIONS: PREPHENATE DEHYDRATASE AND AROGENATE DEHYDRATASE. MAY HAVE A ROLE IN CHEMOTAXIS OR TRANSPORT. InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR001638), solute-binding protein/glutamate receptor (IPR001311) Pfam: Bacterial extracellular solute-binding proteins signal peptide no TMHs; Function unclear; putative cyclohexadienyl dehydratase 537652 pheC 4606433 pheC Azoarcus sp. BH72 putative cyclohexadienyl dehydratase YP_932003.1 536846 R 62928 CDS YP_932004.1 119896791 4609545 complement(537808..539328) 1 NC_008702.1 threonine deaminase; threonine dehydratase; in Escherichia coli, IlvA is part of the isoleucine biosynthetic pathway; threonine dehydratase 539328 ilvA 4609545 ilvA Azoarcus sp. BH72 threonine dehydratase YP_932004.1 537808 R 62928 CDS YP_932005.1 119896792 4609068 complement(539453..540394) 1 NC_008702.1 catalyzes the phosphorylation of the phosphocarrier protein HPr of the bacterial phosphotransferase system; HPr kinase/phosphorylase 540394 hprK 4609068 hprK Azoarcus sp. BH72 HPr kinase/phosphorylase YP_932005.1 539453 R 62928 CDS YP_932006.1 119896793 4609026 complement(540363..540848) 1 NC_008702.1 Probable nitrogen regulatory IIA protein,; High confidence in function and specificity; protein-Npi-phosphohistidine-sugar phosphotransferase 540848 ptsN 4609026 ptsN Azoarcus sp. BH72 protein-Npi-phosphohistidine-sugar phosphotransferase YP_932006.1 540363 R 62928 CDS YP_932007.1 119896794 4609651 complement(540982..541305) 1 NC_008702.1 Probable sigma 54 modulation protein,; High confidence in function and specificity; sigma-54 modulation protein 541305 rpoX 4609651 rpoX Azoarcus sp. BH72 sigma-54 modulation protein YP_932007.1 540982 R 62928 CDS YP_932008.1 119896795 4609803 complement(541320..542780) 1 NC_008702.1 sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma 54 factor is responsible for the expression of enzymes involved in nitrogen assimilation and metabolism; the rhizobia often have 2 copies of this sigma factor; in Rhizobium etli RpoN1 shown to be involved in the assimilation of several nitrogen and carbon sources during free-living aerobic growth and RpoN2 is involved in symbiotic nitrogen fixation; in Bradyrhizobium both RpoN1 and N2 are functional in free-living and symbiotic conditions, rpoN1 gene was regulated in response to oxygen; RNA polymerase factor sigma-54 542780 rpoN1 4609803 rpoN1 Azoarcus sp. BH72 RNA polymerase factor sigma-54 YP_932008.1 541320 R 62928 CDS YP_932009.1 119896796 4609800 complement(542790..543512) 1 NC_008702.1 Probable ABC transporter ATP-binding protein HI1148. ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. TREMBL:Q7NST9: 64% identity,75% similarity InterPro: IPR003593; AAA_ATPase. IPR003439; ABC_transporter. IPR000767; Disease_resist. IPR003016; Lipoyl_BS. Pfam PF00005; ABC_tran; 1 mobB: molybdopterin-guanine dinucleoti TMH's present 0; High confidence in function and specificity; ABC transporter ATPase 543512 yhbG 4609800 yhbG Azoarcus sp. BH72 ABC transporter ATPase YP_932009.1 542790 R 62928 CDS YP_932010.1 119896797 4610180 complement(543499..543948) 1 NC_008702.1 Regulatory protein recX. Modulates recA activity (By similarity). TREMBL: Q7WKM9: 45% identity, 63% similarity InterPro: RecX regulatory protein InterPro; IPR003783; RecX. Pfam: PF02631; RecX PPR: pentatricopeptide repeat domain No transmembrane helices present; Family membership; regulatory protein 543948 recX 4610180 recX Azoarcus sp. BH72 regulatory protein YP_932010.1 543499 R 62928 CDS YP_932011.1 119896798 4609711 complement(543971..545005) 1 NC_008702.1 catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs; recombinase A 545005 recA 4609711 recA Azoarcus sp. BH72 recombinase A YP_932011.1 543971 R 62928 CDS YP_932012.1 119896799 4609702 complement(545130..545615) 1 NC_008702.1 Hypothetical 17.2 kDa protein 1 in recA 5region. TREMBL:Q9KUH9: 54% identity, 64% similarity CinA is the first gene in the competence-inducible (cin) operon, and is thought to be specifically required at some stage in the process of transformation [1]. This is a C-terminal region of putative competence-damaged proteins from the cin operon InterPro:IPR008136; CinA_C. Pfam: PF02464; CinA cinA_cterm: competence/damage-inducible p No signal peptide. No transmembrane helices; High confidence in function and specificity; CinA-like protein 545615 ygaD1 4609702 ygaD1 Azoarcus sp. BH72 CinA-like protein YP_932012.1 545130 R 62928 CDS YP_932013.1 119896800 4610175 complement(545618..546100) 1 NC_008702.1 Region start changed from 546007 to 546100 (93 bases); putative phosphatidylglycerophosphatase A 546100 4610175 azo0509 Azoarcus sp. BH72 putative phosphatidylglycerophosphatase A YP_932013.1 545618 R 62928 CDS YP_932014.1 119896801 4606434 complement(546104..547114) 1 NC_008702.1 Thiamine-phosphate kinase. catalytic activity: atp + thiamine phosphate = adp + thiamine diphosphate. pathway: thiamine biosynthesis.; High confidence in function and specificity; thiamine-phosphate kinase 547114 thiL 4606434 thiL Azoarcus sp. BH72 thiamine-phosphate kinase YP_932014.1 546104 R 62928 CDS YP_932015.1 119896802 4609938 complement(547249..547530) 1 NC_008702.1 hypothetical protein 547530 4609938 azo0511 Azoarcus sp. BH72 hypothetical protein YP_932015.1 547249 R 62928 CDS YP_932016.1 119896803 4606435 complement(547551..548285) 1 NC_008702.1 in Excherichia coli RsxABCDEG reduces SoxR which turns off induction of SoxS transcription factor in the absence of oxidizing agents; similar to the rnfABCDGE operon in Rhodobacter capsulatus involved in transferring electrons to nitrogenase; electron transport complex subunit RsxE 548285 rnfE1 4606435 rnfE1 Azoarcus sp. BH72 electron transport complex subunit RsxE YP_932016.1 547551 R 62928 CDS YP_932017.1 119896804 4609748 complement(548296..548922) 1 NC_008702.1 Probable electron transport complex protein rnfG. Homolog to rnfG of R. capsulata of 51% (sprot|RNFG_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. signal peptide no TMHs; High confidence in function and specificity; electron transport complex protein RnfG 548922 rnfG1 4609748 rnfG1 Azoarcus sp. BH72 electron transport complex protein RnfG YP_932017.1 548296 R 62928 CDS YP_932018.1 119896805 4609750 complement(548939..549994) 1 NC_008702.1 Probable electron transport complex protein rnfD. Homology to rnfD of R. capsulatus of 42% (sprot|RNFD_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase (By similarity). InterPro: NQR2 and RnfD/E related proteins (IPR004338) Pfam: NQR2, RnfD, RnfE family no signal peptide probable 7 TMHs; High confidence in function and specificity; electron transport complex protein RnfD 549994 rnfD1 4609750 rnfD1 Azoarcus sp. BH72 electron transport complex protein RnfD YP_932018.1 548939 R 62928 CDS YP_932019.1 119896806 4609746 complement(549991..551550) 1 NC_008702.1 Probable electron transport complex protein RnfC. Homology to rnfC of R. capsulatus of 48% (sprot|RNFC_RHOCA) Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB. InterPro: Respiratory-chain NADH dehydrogenase 51 Kd subunit (IPR001949); 4Fe-4S ferredoxin, iron-sulfur bidning domain (IPR001450) Pfam: Respiratory-chain NADH dehydrogenase; 4Fe-4S binding domain no signal peptide no TMHs; High confidence in function and specificity; electron transport complex protein RnfC 551550 rnfC1 4609746 rnfC1 Azoarcus sp. BH72 electron transport complex protein RnfC YP_932019.1 549991 R 62928 CDS YP_932020.1 119896807 4609744 complement(551561..552088) 1 NC_008702.1 Probable electron transport complex protein rnfB. Homology to rnfB of R. capsulatus of 48% (sprot|RNFB_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfC. Pfam: 4Fe-4S binding domain signal peptide no TMHs; High confidence in function and specificity; electron transport complex protein RnfB 552088 rnfB1 4609744 rnfB1 Azoarcus sp. BH72 electron transport complex protein RnfB YP_932020.1 551561 R 62928 CDS YP_932021.1 119896808 4609742 complement(552106..552687) 1 NC_008702.1 Probable electron transport complex protein RnfA. Homology to rnfA of R. capsultatus of 66% (RNFA_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Required for stable existence of rnfB and rnfC. InterPro: RnfA-Nqr electron transport subunit (IPR003667) Pfam: Rnf-Nqr subunit, membrane protein no signal peptide 6 TMHs; High confidence in function and specificity; electron transport complex protein RnfA 552687 rnfA1 4609742 rnfA1 Azoarcus sp. BH72 electron transport complex protein RnfA YP_932021.1 552106 R 62928 CDS YP_932022.1 119896809 4609740 553036..554607 1 NC_008702.1 Nitrogen fixation regulatory protein (EC 2.7.3.-). Required for the inhibition of nifA activity in response to oxygen and low level of fixed nitrogen. Similar to SWISSPROT: sprot|NIFL_AZOVI (41% Azotobacter vinelandii,nitrogen fixation regulatory protein (EC 2.7.3.-), NifL) InterPro: IPR003661 His_kinA_N. The histidine kinase A (phosphoacceptor) N-terminal domain is a dimerisation and phosphoacceptor domain of histidine kinases. It has been found in bacterial sensor protein/histidine kinases. IPR000014 PAS. PAS domains are involved in many signalling proteins where they are used as a signal sensor domain. IPR000700 PAS-assoc_C. IPR004358 Bact_sens_pr_C. IPR003594 ATPbind_ATPase. Pfam: PF00512 HisKA. PF00989 PAS. PF00785 PAC. TIGRFAM: TIGR00229 PAS domain S-box.; High confidence in function and specificity; nitrogen fixation regulatory protein 554607 nifL 4609740 nifL Azoarcus sp. BH72 nitrogen fixation regulatory protein YP_932022.1 553036 D 62928 CDS YP_932023.1 119896810 4609365 554604..556157 1 NC_008702.1 Nif-specific regulatory protein. NIFA A TRANSCRIPTIONAL ACTIVATOR IS REQUIRED FOR ACTIVATION OF MOST NIF OPERONS WHICH ARE DIRECTLY INVOLVED IN NITROGEN FIXATION. NIFA INTERACTS WITH SIGMA-54. InterPro: Sigma-54 factor interaction domain; High confidence in function and specificity; nif-specific regulatory protein 556157 nifA 4609365 nifA Azoarcus sp. BH72 nif-specific regulatory protein YP_932023.1 554604 D 62928 CDS YP_932024.1 119896811 4609357 complement(556202..557770) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Gmet02000900 of Geobacter metallireducens of 45% (gi|48846570|ref|ZP_00300831.1|(NBCI ENTREZ)). No domains predicted. Signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 557770 4609357 azo0520 Azoarcus sp. BH72 hypothetical protein YP_932024.1 556202 R 62928 CDS YP_932025.1 119896812 4606436 complement(557796..558329) 1 NC_008702.1 Hypothetical secreted protein. No Hits in the PDB. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 558329 4606436 azo0521 Azoarcus sp. BH72 hypothetical protein YP_932025.1 557796 R 62928 CDS YP_932026.1 119896813 4606437 complement(558326..558853) 1 NC_008702.1 Superoxide dismutase [Cu-Zn]precursor (EC 1.15.1.1), sodC.Destroys radicals which are normally produced within the cells and which are toxic to biological systems. May function against extracytoplasmic toxic oxygen species. 45% SOD_CU_ZN.Copper/Zinc superoxide dismutase. Pfam: PF00080; sodcu; 1. TIGR:CC1579. Signal peptide: present.; High confidence in function and specificity; superoxide dismutase 558853 sodC 4606437 sodC Azoarcus sp. BH72 superoxide dismutase YP_932026.1 558326 R 62928 CDS YP_932027.1 119896814 4609880 559180..560676 1 NC_008702.1 FeMo cofactor biosynthesis protein nifB. PROBABLY INVOLVED IN THE SYNTHESIS OF THE FE-MO COFACTOR.; High confidence in function and specificity; nitrogen fixation protein 560676 nifB 4609880 nifB Azoarcus sp. BH72 nitrogen fixation protein YP_932027.1 559180 D 62928 CDS YP_932028.1 119896815 4609358 560794..561084 1 NC_008702.1 Conserved hypothetical ferredoxin. Homology to fdx of P. stuzeri of 57% (trembl|Q93JV5). Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450) Pfam: 4FE-4S binding domain no signal peptide no TMHs; Family membership; ferredoxin 561084 4609358 azo0524 Azoarcus sp. BH72 ferredoxin YP_932028.1 560794 D 62928 CDS YP_932029.1 119896816 4606438 561181..561630 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of P. stuzeri of 49% (trembl|Q93JV4). Tigrfam: arsC: arsenate reductase. Pfam: ArsC family (PF03960) no signal peptide. no TMHs; hypothetical protein 561630 4606438 azo0525 Azoarcus sp. BH72 hypothetical protein YP_932029.1 561181 D 62928 CDS YP_932030.1 119896817 4606439 561627..562916 1 NC_008702.1 Probable type A flavoprotein fprA . Homology to frpA of R. capsulatus of 58% (sprot|FPRA_RHOCA) Low-potential electron donor to a number of redox enzymes (Potential). InterPro: Metallo-beta-lactamase superfamily (IPR001279); Flavodoxin (IPR001226) Pfam: Metallo-beta-lactamase superfamily; Flavodoxin no singal peptide no TMHs; Family membership; type A flavoprotein 562916 fprA 4606439 fprA Azoarcus sp. BH72 type A flavoprotein YP_932030.1 561627 D 62928 CDS YP_932031.1 119896818 4608826 562926..563213 1 NC_008702.1 Ferredoxin IV (FdIV) (Ferredoxin plant-type). Homology to fdxC of R. capsulatus of 62% (FER4_RHOCA). FERREDOXINS ARE IRON-SULFUR PROTEINS THAT TRANSFER ELECTRONS IN A WIDE VARIETY OF METABOLIC REACTIONS. THIS FERREDOXIN PROBABLY PARTICIPATES IN NITROGEN FIXATION. Pfam: 2Fe-2S iron-sulfur clauster binding domain no signal peptide no TMHs; High confidence in function and specificity; ferredoxin IV 563213 fdxC 4608826 fdxC Azoarcus sp. BH72 ferredoxin IV YP_932031.1 562926 D 62928 CDS YP_932032.1 119896819 4608749 563265..563615 1 NC_008702.1 Ferredoxin V (FdV) (Ferredoxin plant-type). FERREDOXINS ARE IRON-SULFUR PROTEINS THAT TRANSFER ELECTRONS IN A WIDE VARIETY OF METABOLIC REACTIONS. THIS FERREDOXIN PROBABLY PARTICIPATES IN NITROGEN FIXATION. InterPro: Ferredoxin; High confidence in function and specificity; ferredoxin V 563615 fdxD 4608749 fdxD Azoarcus sp. BH72 ferredoxin V YP_932032.1 563265 D 62928 CDS YP_932033.1 119896820 4608750 563680..564255 1 NC_008702.1 Putative NifQ protein. Homology to nifQ of A. vinelandii of 33% (sprot|NIFQ_AZOVI(SRS) NifQ is involved in early stages of the biosynthesis of the iron-molybdenum cofactor (FeMo-co), which is an integral part of the active site of dinitrogenase [2]. The conserved C-terminal cysteine residues may be involved in metal binding. Pfam: NifQ no signal peptide no TMHs; Family membership; putative NifQ protein 564255 nifQ 4608750 nifQ Azoarcus sp. BH72 putative NifQ protein YP_932033.1 563680 D 62928 CDS YP_932034.1 119896821 4609369 564301..565239 1 NC_008702.1 Probable ADP-ribosyl-[dinitrogen reductase] hydrolase. Homology to draG of R. rubrum of 44% (sprot|DRAG_RHORU) Involved in the regulation of the nitrogen fixation activity by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex. The ADP-ribosyltransferase (DraT) transfers the ADP-ribose group from NAD to dinitrogenase reductase. The ADP-ribose group is removed through the action of the ADP-ribosylglycohydrolase (DraG). Pfam: ADP-ribosylglycohydrolase no signal peptide no TMHs; ADP-ribosyl-[dinitrogen reductase] hydrolase 565239 draG1 4609369 draG1 Azoarcus sp. BH72 ADP-ribosyl-[dinitrogen reductase] hydrolase YP_932034.1 564301 D 62928 CDS YP_932035.1 119896822 4608648 complement(565267..566196) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA2778 of P.aeruginosa of 39% (trembl|Q9I065(SRS)) Has Signal Peptide. No TMH present. Has PF03412:Peptidase C39 family;Lantibiotic and non-lantibiotic bacteriocins are synthesised as precursor peptides containing N-terminal extensions (leader peptides) which are cleaved off during maturation. Most non-lantibiotics and also some lantibiotics have leader peptides of the so-called double-glycine type. These leader peptides share consensus sequences and also a common processing site with two conserved glycine residues in positions -1 and -2. The double- glycine-type leader peptides are unrelated to the N-terminal signal sequences which direct proteins across the cytoplasmic membrane via the sec pathway. Their processing sites are also different from typical signal peptidase cleavage sites, suggesting that a different processing enzyme is involved. Peptide bacteriocins are exported across the cytoplasmic membrane by a dedicated ATP-binding cassette (ABC) transporter. The ABC transporter is the maturation protease and its proteolytic domain resides in the N-terminal part of the protein. This peptidase domain is found in a wide range of ABC transporters, however the presumed catalytic cysteine and histidine are not conserved in all members of this family.; Conserved hypothetical protein; hypothetical protein 566196 4608648 azo0531 Azoarcus sp. BH72 hypothetical protein YP_932035.1 565267 R 62928 CDS YP_932036.1 119896823 4606440 complement(566198..566773) 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein with the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 566773 4606440 azo0532 Azoarcus sp. BH72 hypothetical protein YP_932036.1 566198 R 62928 CDS YP_932037.1 119896824 4606441 complement(566665..567276) 1 NC_008702.1 Hypothetical protein yjdF,44% identity (56% similarity) to TrEMBL;Q8XDT2. SwissProt;P39270. Signal P reporting Signal peptide present. TMHMM2 reporting 3 TMH's present.; hypothetical protein 567276 yjdF 4606441 yjdF Azoarcus sp. BH72 hypothetical protein YP_932037.1 566665 R 62928 CDS YP_932038.1 119896825 4610187 complement(567329..568462) 1 NC_008702.1 Hypothetical membrane protein. TREMBL:Q83LX6: 27% identity, 42% similarity. similarity to E.coli ybeT. some,to yeast skt5 and s.pombe spac24b11.10c InterPro; IPR006597; Sel_like. InterPro; IPR001440; TPR. InterPro; IPR008941; TPR-like Pfam:DsbD:Cytochrome C biogenesis protein trans SMART:SM00671; SEL1 Signal peptide present (Signal P) transmembrane helices: 5 (TMHMM predicted).; Function unclear; hypothetical protein 568462 4610187 azo0534 Azoarcus sp. BH72 hypothetical protein YP_932038.1 567329 R 62928 CDS YP_932039.1 119896826 4606442 complement(568662..569072) 1 NC_008702.1 DcrH: hemerythrin protein,is a transmembrane methyl-accepting protein probably involved in bacterial chemotaxis. 36% Hemerythrin. Hemerythrin family non-heme Pfam:PF01814; Hemerythrin; 1.; High confidence in function and specificity; putative hemerythrin-like protein 569072 dcrH1 4606442 dcrH1 Azoarcus sp. BH72 putative hemerythrin-like protein YP_932039.1 568662 R 62928 CDS YP_932040.1 119896827 4608590 complement(569083..569934) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Pseudomonas stutzeri of 52% (tremble:Q9EVM9). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 569934 4608590 azo0536 Azoarcus sp. BH72 hypothetical protein YP_932040.1 569083 R 62928 CDS YP_932041.1 119896828 4606443 complement(570131..570973) 1 NC_008702.1 Putative NAD(+)-dinitrogen-reductase ADP-D-ribosyltransferase (EC 2.4.2.37) (ADP-ribosyltransferase). Homology to draT of R. rubrum of 39% (sprot|DRAT_RHORU(SRS) Involved in the regulation of the nitrogen fixation activity by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex. The ADP-ribosyltransferase transfers the ADP-ribose group from NAD to dinitrogenase reductase. The ADP-ribose group is removed through the action of the ADP-ribosylglycohydrolase (DraG). Pfam: DRAT no signal peptide no TMHs; High confidence in function and specificity; putative NAD(+)-dinitrogen-reductase ADP-D-ribosyltransferase 570973 draT 4606443 draT Azoarcus sp. BH72 putative NAD(+)-dinitrogen-reductase ADP-D-ribosyltransferase YP_932041.1 570131 R 62928 CDS YP_932042.1 119896829 4608650 571215..572108 1 NC_008702.1 88% NifH.IPR000392; NitrogenaseII. Pfam:PF00142; Fer4_NifH; 1. TIGRFAMs:TIGR02016; BchX; 1.TIGR01287; nifH; 1.; High confidence in function and specificity; putative nitrogenase iron protein 572108 nifH 4608650 nifH Azoarcus sp. BH72 putative nitrogenase iron protein YP_932042.1 571215 D 62928 CDS YP_932043.1 119896830 4609363 572230..573705 1 NC_008702.1 Nitrogenase molybdenum-iron protein alpha chain (EC 1.18.6.1) (Nitrogenase component I) (Dinitrogenase). The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex which has 2 components: the iron protein and the molybdenum-iron protein.; High confidence in function and specificity; nitrogenase molybdenum-iron protein subunit alpha 573705 nifD 4609363 nifD Azoarcus sp. BH72 nitrogenase molybdenum-iron protein subunit alpha YP_932043.1 572230 D 62928 CDS YP_932044.1 119896831 4609359 573809..575377 1 NC_008702.1 Nitrogenase molybdenum-iron protein beta chain (EC 1.18.6.1) (Nitrogenase component I) (Dinitrogenase). The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex which has 2 components: the iron protein and the molybdenum-iron protein. InterPro: Oxidoreductase nitrogenase component 1 araD: L-ribulose-5-phosphate 4-epimer; High confidence in function and specificity; nitrogenase molybdenum-iron protein subunit beta 575377 nifK 4609359 nifK Azoarcus sp. BH72 nitrogenase molybdenum-iron protein subunit beta YP_932044.1 573809 D 62928 CDS YP_932045.1 119896832 4609364 575484..575708 1 NC_008702.1 Nitrogen fixation protein [nifT],51% identity(72% Similarity) to SwissProt;P09427, TrEMBL;Q93JU8,57% identity. Has PF06988:NifT/FixU protein(IPR009727);This family consists of several NifT and FixU bacterial proteins. The function of NifT is unknown although it is thought that the protein may be involved in biosynthesis of the FeMo cofactor of nitrogenase although perturbation of nifT expression in K. pneumoniae has only a limited effect on nitrogen fixation. No Signal peptide or TMH present.; High confidence in function and specificity; nitrogen fixation protein NifT 575708 nifT 4609364 nifT Azoarcus sp. BH72 nitrogen fixation protein NifT YP_932045.1 575484 D 62928 CDS YP_932046.1 119896833 4609371 575735..575938 1 NC_008702.1 Ferredoxin. Pubilication: Egner et al., 2001, J Bacteriol 183, 3752-3760 (trembl|Q9F0V6) Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450) Pfam: 4Fe-4S binding daimain no signal peptide no TMHs; High confidence in function and specificity; FdxN protein 575938 fdxN 4609371 fdxN Azoarcus sp. BH72 FdxN protein YP_932046.1 575735 D 62928 CDS YP_932047.1 119896834 4608751 576021..576743 1 NC_008702.1 NifY protein. Homology to nifY of Azoarcus sp. BH72 of 99% (gi|11493644|gb|AF200742.1|AF200742(NBCI ENTREZ)). Pfam: Dinitrogenase iron-molybdenum cofactor. This family contains several NIF (B, Y and X) proteins which are involved in the synthesis of an iron-molybdenum cofactors (FeMo-co) in the dinitrogenase enzyme which catalyses the reduction of dinitrogen to ammonium. No signal peptide predicted. No TMHs.; Function unclear; NifY protein 576743 nifY1 4608751 nifY1 Azoarcus sp. BH72 NifY protein YP_932047.1 576021 D 62928 CDS YP_932048.1 119896835 4609376 576740..577012 1 NC_008702.1 ORF1; Conserved hypothetical protein. Homology to orf1 of Azoarcus sp. BH72 of 100% (gi|11493650|gb|AAG35591.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 577012 4609376 azo0544 Azoarcus sp. BH72 hypothetical protein YP_932048.1 576740 D 62928 CDS YP_932049.1 119896836 4606444 577211..578842 1 NC_008702.1 Putative methyl-accepting chemotaxis protein,Chmtaxis_transd. Pfam: PF00015; MCPsignal. SMART: SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Specificity unclear; putative methyl-accepting chemotaxis protein 578842 4606444 azo0545 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_932049.1 577211 D 62928 CDS YP_932050.1 119896837 4606445 complement(578928..579785) 1 NC_008702.1 Probable peptidylprolyl isomerase. Homology with nifM of A. chroococcum of 43% (sprot|NIFM_AZOCH) REQUIRED FOR THE ACTIVATION AND STABILIZATION OF THE IRON-COMPONENT (NIFH) OF NITROGENASE. PROBABLE PPIASE. InterPro: PpiC-type peptidyl-prolyl cis-trans isomerase (IPR000297) Pfam: PPIC-type PPIASE domain no signal peptide no TMHs hydrog_prot: hydrogenase maturation pro; High confidence in function and specificity; peptidyl-prolyl isomerase 579785 nifM 4606445 nifM Azoarcus sp. BH72 peptidyl-prolyl isomerase YP_932050.1 578928 R 62928 CDS YP_932051.1 119896838 4609366 complement(579782..580273) 1 NC_008702.1 Nitrogen Fixation protein[nifZ], 51% identity (67% similarity) SwissProt;P14889,66% similarity to SwissProt;P23124. Has PF04319:NifZ domain;(IPR007415)This short protein is found in the nif (nitrogen fixation) operon. Its function is unknown but is probably involved in nitrogen fixation or regulating some component of this process. This 75 residue region is presumed to be a domain. It is found in isolation in some members and in the amino terminal half of the longer NifZ proteins. No Signal Peptide Or TMH reported Present.; High confidence in function and specificity; nitrogen fixation protein 580273 nifZ 4609366 nifZ Azoarcus sp. BH72 nitrogen fixation protein YP_932051.1 579782 R 62928 CDS YP_932052.1 119896839 4609378 complement(580294..580614) 1 NC_008702.1 Nitrogenase stabilizing/protective protein nifW,50% identity (68% similarity) to SwissProt;P14888.TrEMBL; Q6US88(60% similarity). Has PF03206:Nitrogen fixation protein NifW;(IPR004893)Nitrogenase is a complex metalloenzyme composed of two proteins designated the Fe-protein and the MoFe-protein. Apart from these two proteins, a number of accessory proteins are essential for the maturation and assembly of nitrogenase. Even though experimental evidence suggests that these accessory proteins are required for nitrogenase activity, the exact roles played by many of these proteins in the functions of nitrogenase are unclear. Using yeast two-hybrid screening it has been shown that NifW can interact with itself as well as NifZ. No Signal Peptide or TMH present.; High confidence in function and specificity; nitrogen fixation protein NifW 580614 nifW 4609378 nifW Azoarcus sp. BH72 nitrogen fixation protein NifW YP_932052.1 580294 R 62928 CDS YP_932053.1 119896840 4609374 complement(580638..581177) 1 NC_008702.1 Conserved hypothetical protein. Homology to orf8 of Azotobacter vinelandii of 36% (tremble:Q44542). No domains predicted. No Signal peptide or TMH present.; hypothetical protein 581177 4609374 azo0549 Azoarcus sp. BH72 hypothetical protein YP_932053.1 580638 R 62928 CDS YP_932054.1 119896841 4606446 complement(581177..582025) 1 NC_008702.1 Serine acetyltransferase (EC 2.3.1.30). Homology to nifP of A. chrooccum of 58% (pir|D43706). PROBABLE SERINE ACETYLTRANSFERASE REQUIRED FOR OPTIMIZING THE EXPRESSION OF NITROGENASE ACTIVITY. NIFP MAY BE REQUIRED TO BOOST RATES OF SYNTHESIS OR INTRACELLULAR CONCENTRATIONS OF CYSTEINE OR METHIONINE. InterPro: Bacterial transferase hexapeptide repeat (IPR001451) no signal peptide no TMHs; High confidence in function and specificity; serine O-acetyltransferase 582025 nifP 4606446 nifP Azoarcus sp. BH72 serine O-acetyltransferase YP_932054.1 581177 R 62928 CDS YP_932055.1 119896842 4609368 complement(582028..583218) 1 NC_008702.1 Homocitrate synthase is involved in the biosynthesis of the iron-molybdenum cofactor of nitrogenase and catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate into homocitrate. Similar to sprot|NIFV_AZOVI (51%) and to sprot|NIV1_ANASP (42%). Pfam (PF00682): HMG-CoA Lyase-like family InterPro (PS00815): Alpha-isopropylmalate and homocitrate synthase; High confidence in function and specificity; homocitrate synthase 583218 nifV 4609368 nifV Azoarcus sp. BH72 homocitrate synthase YP_932055.1 582028 R 62928 CDS YP_932056.1 119896843 4609373 complement(583307..584530) 1 NC_008702.1 Cysteine desulfurase(Nitrogenase metalloclusters biosynthesis protein nifS). Homology to nifS of A. chroococcum of 71% (sprot|NIFS_AZOCH) CATALYZES THE REMOVAL OF ELEMENTAL SULFUR ATOMS FROM CYSTEINE TO PRODUCE ALANINE. SEEMS TO PARTICIPATE IN THE BIOSYNTHESIS OF THE NITROGENASE METALLOCLUSTERS BY PROVIDING THE INORGANIC SULFUR REQUIRED FOR THE FE-S CORE FORMATION. Pfam: aminotransferase class-V no signal peptide no TMHs; High confidence in function and specificity; cysteine desulfurase 584530 nifS 4609373 nifS Azoarcus sp. BH72 cysteine desulfurase YP_932056.1 583307 R 62928 CDS YP_932057.1 119896844 4609370 complement(584713..585603) 1 NC_008702.1 Probable nitrogen fixation protein NifU. Homology to nifU of A. vinelandii of 67% (sprot|NIFU_AZOVI). INVOLVED IN THE FORMATION OR REPAIR OF [FE-S] CLUSTERS PRESENT IN IRON-SULFUR PROTEINS. Pfam: NifU-like N-terminal domain, NifU-like domain no signal peptide no TMHs; High confidence in function and specificity; nitrogen fixation protein NifU 585603 nifU 4609370 nifU Azoarcus sp. BH72 nitrogen fixation protein NifU YP_932057.1 584713 R 62928 CDS YP_932058.1 119896845 4609372 complement(585625..585948) 1 NC_008702.1 HesB/yadR/yfhF family protein, 45% identity to TrEMBL;Q9KJL2,Q6HRJ0. SwissProt;P37026(41% identity) Has PF01521, HesB-like domain;IPR000361, HesB_yadR_yfhF;This family includes HesB which may be involved in nitrogen fixation; the hesB gene is expressed only under nitrogen fixation conditions. Other members of this family include various hypothetical proteins of which P46847 and NP31774 also contains NifU-like domains NifU which is also involved in nitrogen fixation. In the gram-negative soil bacterium Rhizobium etli, the hesB-like gene iscN is required for nitrogen fixation.; High confidence in function and specificity; HesB/yadR/yfhF family protein 585948 hesB 4609372 hesB Azoarcus sp. BH72 HesB/yadR/yfhF family protein YP_932058.1 585625 R 62928 CDS YP_932059.1 119896846 4608991 complement(586209..586601) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03002610 of Dechloromonas aromatica of 39% (gi|41724013|ref|ZP_00150903.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 586601 4608991 azo0555 Azoarcus sp. BH72 hypothetical protein YP_932059.1 586209 R 62928 CDS YP_932060.1 119896847 4606447 complement(586611..586898) 1 NC_008702.1 Probable ferredoxin. Homology to fdxB of R. capsulatus of 45% (sprot|FER3_RHOCA) Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450) Pfam: 4Fe-4S binding domain no signal peptide no TMHs; Family membership; ferredoxin 586898 fdxB 4606447 fdxB Azoarcus sp. BH72 ferredoxin YP_932060.1 586611 R 62928 CDS YP_932061.1 119896848 4608748 complement(587039..587254) 1 NC_008702.1 Conserved hypothetical protein Shows similarity to hypothetical 7.7 kDa protein in fixX 3'region (ORF1) of Azorhizobium caulinodans. TIGR00162: conserved hypothetical protein; hypothetical protein 587254 4608748 azo0557 Azoarcus sp. BH72 hypothetical protein YP_932061.1 587039 R 62928 CDS YP_932062.1 119896849 4606448 complement(587265..587750) 1 NC_008702.1 Conserved hypothetical protein(Has probable Nif-associated protein function)41% identity(62% similarity) to TrEMBL;Q9KJL5. Has PF03270(IPR004952):Protein of unknown function, DUF269;Members of this family may be involved in nitrogen fixation, since they are found within nitrogen fixation operons. NO Signal Peptide or TMH present.; Family membership; hypothetical protein 587750 4606448 azo0558 Azoarcus sp. BH72 hypothetical protein YP_932062.1 587265 R 62928 CDS YP_932063.1 119896850 4606449 complement(587769..588176) 1 NC_008702.1 Hypothetical sigma-E factor regulatory protein,best similarity to SWISSPROT: sprot|Y850_HAEIN (Haemophilus influenzae, hypothetical protein hi0850) / sprot|RSEC_ECOLI (23% Escherichia coli, sigma-e factor regulatory protein RseC). TMHMM reporting 2 transmembrane helices.; sigma-E factor regulatory protein 588176 4606449 azo0559 Azoarcus sp. BH72 sigma-E factor regulatory protein YP_932063.1 587769 R 62928 CDS YP_932064.1 119896851 4606450 complement(588173..588700) 1 NC_008702.1 Conserved hypothetical NifX protein. Homology to nifX of Azotobacter vinelandii of 47% (gi|128328|sp|P14887|NIFX_AZOVI(NBCI ENTREZ)). Pfam: Dinitrogenase iron-molybdenum cofactor. This family contains several NIF (B, Y and X) proteins which are involved in the synthesis of an iron-molybdenum cofactors (FeMo-co) in the dinitrogenase enzyme which catalyses the reduction of dinitrogen to ammonium. No signal peptide predicted. No TMHs.; Conserved hypothetical protein; NifX protein 588700 nifX 4606450 nifX Azoarcus sp. BH72 NifX protein YP_932064.1 588173 R 62928 CDS YP_932065.1 119896852 4609375 complement(588618..590012) 1 NC_008702.1 functions with NifE to assemble FeMo cofactor; functions in assembly of nitrogenase MoFe; nitrogenase molybdenum-cofactor biosynthesis protein NifN 590012 nifN 4609375 nifN Azoarcus sp. BH72 nitrogenase molybdenum-cofactor biosynthesis protein NifN YP_932065.1 588618 R 62928 CDS YP_932066.1 119896853 4609367 complement(590023..591525) 1 NC_008702.1 Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE. Homology to nifE of A. vielandii of 70% (sprot|NIFE_AZOVI). This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor). InterPro: Oxidoreductase nitrogenase component 1 (IPR000510) Pfam: Nitrogenease component 1 type oxidoreductase no signal peptide no TMHs; High confidence in function and specificity; nitrogenase iron-molybdenum cofactor biosynthesis protein NifE 591525 nifE 4609367 nifE Azoarcus sp. BH72 nitrogenase iron-molybdenum cofactor biosynthesis protein NifE YP_932066.1 590023 R 62928 CDS YP_932067.1 119896854 4609360 complement(591714..592715) 1 NC_008702.1 Probale Hypothetical protein. Very Weak homology spanning the entire length of protein in the data base. No Significant domains,Features,TMH's or Signal Peptide present.; Function unclear; hypothetical protein 592715 4609360 azo0563 Azoarcus sp. BH72 hypothetical protein YP_932067.1 591714 R 62928 CDS YP_932068.1 119896855 4606451 complement(592764..593639) 1 NC_008702.1 Conserved hypothetical protein. Has PF05484:LRV protein FeS4 cluster(IPR008665);This Iron sulphur cluster is found at the N-terminus of some proteins containing LRV repeats. Has 6,(IPR004830) PF01816:Leucine rich repeat variant;The function of this repeat is unknown. It has an unusual structure of two helices. One is an alpha helix,the other is the much rarer 3-10 helix.; Function unclear; hypothetical protein 593639 4606451 azo0564 Azoarcus sp. BH72 hypothetical protein YP_932068.1 592764 R 62928 CDS YP_932069.1 119896856 4606452 593892..597104 1 NC_008702.1 GGDEF/EAL/PAS/PAC/GAF-domain containing protein; diguanylate cyclase 597104 morA 4606452 morA Azoarcus sp. BH72 diguanylate cyclase YP_932069.1 593892 D 62928 CDS YP_932070.1 119896857 4609260 597165..597449 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 597449 4609260 azo0566 Azoarcus sp. BH72 hypothetical protein YP_932070.1 597165 D 62928 CDS YP_932071.1 119896858 4606453 complement(597475..598143) 1 NC_008702.1 Similar to TREMBL:Q82SF8 (45% identity); TREMBL:Q7P0W9 (43% identity); TREMBL:Q8XV73 (40% identity).; hypothetical protein 598143 4606453 azo0567 Azoarcus sp. BH72 hypothetical protein YP_932071.1 597475 R 62928 CDS YP_932072.1 119896859 4606454 598310..599704 1 NC_008702.1 Putative sensory box histidine kinase,; High confidence in function and specificity; putative sensory box histidine kinase 599704 vsrA 4606454 vsrA Azoarcus sp. BH72 putative sensory box histidine kinase YP_932072.1 598310 D 62928 CDS YP_932073.1 119896860 4610096 complement(599710..600345) 1 NC_008702.1 DNA-binding response regulator,; High confidence in function and specificity; DNA-binding response regulator 600345 vsrD1 4610096 vsrD1 Azoarcus sp. BH72 DNA-binding response regulator YP_932073.1 599710 R 62928 CDS YP_932074.1 119896861 4610098 complement(600348..601409) 1 NC_008702.1 Similar to TREMBL:Q89KB0 (34% identity); TREMBL:Q8XXG5 (34% identity).; hypothetical protein 601409 4610098 azo0570 Azoarcus sp. BH72 hypothetical protein YP_932074.1 600348 R 62928 CDS YP_932075.1 119896862 4610009 complement(601871..602422) 1 NC_008702.1 endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity; Holliday junction resolvase 602422 ruvC 4610009 ruvC Azoarcus sp. BH72 Holliday junction resolvase YP_932075.1 601871 R 62928 CDS YP_932076.1 119896863 4609837 complement(602457..602777) 1 NC_008702.1 Conserved hypothetical protein, ybjQ, 65% identity to SwissProt;P75819. SwiisProt;Q8XGV2(66% identity). TrEMBL;Q83LS2( 65% identity) Has PF01906, Domain of unknown function DUF74;IPR002765; Members of this protein family have no known function. The domain is about 100 amino acids long and found in prokaryotes.; High confidence in function and specificity; hypothetical protein 602777 ybjQ 4609837 ybjQ Azoarcus sp. BH72 hypothetical protein YP_932076.1 602457 R 62928 CDS YP_932077.1 119896864 4610151 complement(602808..603686) 1 NC_008702.1 Conserved hypothetical membrane protein. Hamology to PA3358 of P. aeruginosa of 56% InterPro: Integral membrane protein DUF6 Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transporter Pfam: Integral membrane protein DUF6 no signal peptide probable 10 TMHs; Conserved hypothetical protein; hypothetical protein 603686 4610151 azo0573 Azoarcus sp. BH72 hypothetical protein YP_932077.1 602808 R 62928 CDS YP_932078.1 119896865 4606456 complement(603781..604506) 1 NC_008702.1 Conserved hypothetical protein,yebC,48% identity (63% similarity) to SwissProt;Q8FGR1,P24237,Q8XFD4. TrEMBL;Q9S4S9. Has PF01709, Domain of unknown function DUF28;IPR002876; This domain is found in bacterial and yeast proteins it compromises the entire length or central region of most of the proteins in the family, all of which are hypothetical with no known function. The average length of this domain is approximately 230 amino acids long.; High confidence in function and specificity; hypothetical protein 604506 yebC 4606456 yebC Azoarcus sp. BH72 hypothetical protein YP_932078.1 603781 R 62928 CDS YP_932079.1 119896866 4610169 604637..605281 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide Present.; hypothetical protein 605281 4610169 azo0575 Azoarcus sp. BH72 hypothetical protein YP_932079.1 604637 D 62928 CDS YP_932080.1 119896867 4606457 605320..606831 1 NC_008702.1 Conserved hypothetical protein. Homology to yjgR of E. coli of 66% (sprot|YJGR_ECOLI). no signal peptide no TMHs; Family membership; hypothetical protein 606831 4606457 azo0576 Azoarcus sp. BH72 hypothetical protein YP_932080.1 605320 D 62928 CDS YP_932081.1 119896868 4606458 complement(606869..607759) 1 NC_008702.1 Probable transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 607759 gstR2 4606458 gstR2 Azoarcus sp. BH72 LysR family transcriptional regulator YP_932081.1 606869 R 62928 CDS YP_932082.1 119896869 4608960 607889..609352 1 NC_008702.1 Multidrug resistance protein B homolog. TRANSLOCASE THAT CONFERS RESISTANCE TO SUBSTANCES OF HIGH HYDROPHOBICITY (BY SIMILARITY). Presence of signal peptide and 14 transmembrane helices. Presence of DUF domains,CbiM, and bacterial cytochrome ubiquinol oxidase like domains efflux_EmrB: drug resistance transport; High confidence in function and specificity; multidrug resistence transmembrane protein 609352 emrY 4608960 emrY Azoarcus sp. BH72 multidrug resistence transmembrane protein YP_932082.1 607889 D 62928 CDS YP_932083.1 119896870 4608667 609429..610385 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RSc2187 of R. solanacerarum of 44%. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transporter Pfam: Intergral membrane protein DUF6 no signal peptide probable 6 TMHs; Conserved hypothetical protein; hypothetical protein 610385 4608667 azo0579 Azoarcus sp. BH72 hypothetical protein YP_932083.1 609429 D 62928 CDS YP_932084.1 119896871 4606459 610477..612138 1 NC_008702.1 Putative methyl-accepting chemotaxis transducer,Chmtaxis_transd. Pfam: PF00015; MCPsignal. SMART: SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 1 transmembrane helices. Methyl-accepting chemotaxis serine transducer. CHEMOTACTIC-SIGNAL TRANSDUCERS RESPOND TO CHANGES IN THE CONCENTRATION OF ATTRACTANTS AND REPELLENTS IN THE ENVIRONMENT TRANSDUCE A SIGNAL FROM THE OUTSIDE TO THE INSIDE OF THE CELL AND FACILITATE SENSORY ADAPTATION THROUGH THE VARIATION OF THE LEVEL OF METHYLATION. ATTRACTANTS INCREASE THE LEVEL OF METHYLATION WHILE REPELLENTS DECREASE THE LEVEL OF METHYLATION THE METHYL GROUPS ARE ADDED BY THE METHYLTRANSFERASE CHER AND REMOVED BY THE METHYLESTERASE CHEB. recN: DNA repair protein RecN; Specificity unclear; putative methyl-accepting chemotaxis transducer 612138 4606459 azo0580 Azoarcus sp. BH72 putative methyl-accepting chemotaxis transducer YP_932084.1 610477 D 62928 CDS YP_932085.1 119896872 4606460 complement(612146..613330) 1 NC_008702.1 May be involved in either the transport or processing of arabinose polymers.Belongs to the major facilitator superfamily. 36% MFS.IPR005828; Sub_transporter. TMhelix:12.; High confidence in function and specificity; putative transmembrane efflux protein 613330 araJ 4606460 araJ Azoarcus sp. BH72 putative transmembrane efflux protein YP_932085.1 612146 R 62928 CDS YP_932086.1 119896873 4608330 complement(613443..614654) 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. 25% MFS.IPR005828; Sub_transporter.IPR005829; Sug_transporter.InterPro: General substrate transporters Pfam:PF00083; sugar_tr; 1. TMHelix: 12; Specificity unclear; major facilitator superfamily permease 614654 4608330 azo0582 Azoarcus sp. BH72 major facilitator superfamily permease YP_932086.1 613443 R 62928 CDS YP_932087.1 119896874 4606461 614751..615716 1 NC_008702.1 Putative transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 615716 lysR 4606461 lysR Azoarcus sp. BH72 LysR family transcriptional regulator YP_932087.1 614751 D 62928 CDS YP_932088.1 119896875 4609187 complement(615741..616574) 1 NC_008702.1 510-methylenetetrahydrofolate reductase InterPro: Methylenetetrahydrofolate reductase fadh2: 510-methylenetetrahydrofolate reductase; High confidence in function and specificity; 5,10-methylenetetrahydrofolate reductase 616574 metF 4609187 metF Azoarcus sp. BH72 5,10-methylenetetrahydrofolate reductase YP_932088.1 615741 R 62928 CDS YP_932089.1 119896876 4609208 complement(616561..617238) 1 NC_008702.1 Probable hemolysin.46% identity to TrEMBL;Q7NQ39. Has PF01728, FtsJ-like methyltransferase; IPR002877,RrmJFtsJ_mtfrase; This family consists of FtsJ from various bacterial and archaeal sources FtsJ is a methyltransferase, but actually has no effect on cell division. FtsJ's substrate is the 23S rRNA. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. This family also includes the N terminus of flaviviral NS5 protein. It has been hypothesised that the N-terminal domain of NS5 is a methyltransferase involved in viral RNA capping.; High confidence in function and specificity; hypothetical protein 617238 tlyA 4609208 tlyA Azoarcus sp. BH72 hypothetical protein YP_932089.1 616561 R 62928 CDS YP_932090.1 119896877 4609952 complement(617454..617696) 1 NC_008702.1 Hypothetical protein. Weak Homology with hits in the Database. No domains, repeats, motifs or features could be predicted above threshold value.; hypothetical protein 617696 4609952 azo0586 Azoarcus sp. BH72 hypothetical protein YP_932090.1 617454 R 62928 CDS YP_932091.1 119896878 4606462 complement(617790..619199) 1 NC_008702.1 catalyzes the formation of L-homocysteine from S-adenosyl-L-homocysteine; S-adenosyl-L-homocysteine hydrolase 619199 acyH 4606462 acyH Azoarcus sp. BH72 S-adenosyl-L-homocysteine hydrolase YP_932091.1 617790 R 62928 CDS YP_932092.1 119896879 4608276 619475..620047 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA0776 of Pseudomonas aeruginosa of 30% (trembl|Q9I5G2(SRS)) No domains predicted Signal Peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 620047 4608276 azo0588 Azoarcus sp. BH72 hypothetical protein YP_932092.1 619475 D 62928 CDS YP_932093.1 119896880 4606463 complement(620087..621253) 1 NC_008702.1 catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase; S-adenosylmethionine synthetase 621253 metK 4606463 metK Azoarcus sp. BH72 S-adenosylmethionine synthetase YP_932093.1 620087 R 62928 CDS YP_932094.1 119896881 4609210 621534..622355 1 NC_008702.1 Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA; lipid A biosynthesis lauroyl acyltransferase 622355 htrB 4609210 htrB Azoarcus sp. BH72 lipid A biosynthesis lauroyl acyltransferase YP_932094.1 621534 D 62928 CDS YP_932095.1 119896882 4609041 622384..623160 1 NC_008702.1 Putative Lipid A biosynthesis acyltransferase Homology to msbB of of E. coli of 27% (AAA24181). Homology only with the N-terminus. no signal peptide. 1 TMH. Tigrfam: lipid_A_htrB, lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase. Pfam: Lip_A_acyltrans,Bacterial lipid A biosynthesis acyltransferase.; Family membership; putative lipid A biosynthesis acyltransferase,truncated 623160 msbBa 4609041 msbBa Azoarcus sp. BH72 putative lipid A biosynthesis acyltransferase,truncated YP_932095.1 622384 D 62928 CDS YP_932096.1 119896883 4609277 623157..623261 1 NC_008702.1 Putative Lipid A biosynthesis acyltransferase Homology to.msbB of of E. coli of 27% (AAA24181). Homology only with the N-terminus. no signal peptide. 1 TMH. Tigrfam: lipid_A_htrB, lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase. Pfam: Lip_A_acyltrans,Bacterial lipid A biosynthesis acyltransferase.; Function unclear; putative lipid A biosynthesis acyltransferase,truncated 623261 msbBb 4609277 msbBb Azoarcus sp. BH72 putative lipid A biosynthesis acyltransferase,truncated YP_932096.1 623157 D 62928 CDS YP_932097.1 119896884 4609278 complement(623255..623521) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03000819 of Dechloromonas aromatica of 32% (gi|46140899|ref|ZP_00152594.2|(NBCI ENTREZ)). No domains predicted. signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 623521 4609278 azo0593 Azoarcus sp. BH72 hypothetical protein YP_932097.1 623255 R 62928 CDS YP_932098.1 119896885 4606464 623683..624516 1 NC_008702.1 involved in lysine biosynthesis; DAP epimerase; produces DL-diaminopimelate from LL-diaminopimelate; diaminopimelate epimerase 624516 dapF 4606464 dapF Azoarcus sp. BH72 diaminopimelate epimerase YP_932098.1 623683 D 62928 CDS YP_932099.1 119896886 4608585 624557..625210 1 NC_008702.1 Conserved hypothetical protein. Coils2 Program reporting presence of 1 Coiled-Coil. Has SMART;SM00065,GAF domain;This domain is present in phytochromes and cGMP-specific phosphodiesterases. cGMP-dependent 3',5'-cyclic phosphodiesterase catalyses the conversion of guanosine 3',5'-cyclic phosphate to guanosine 5'-phosphate. A phytochrome is a regulatory photoreceptor which exists in 2 forms that are reversibly interconvertible by light, the PR form that absorbs maximally in the red region of the spectrum, and the PFR form that absorbs maximally in the far-red region. This domain is also found in NifA, a transcriptional activator which is required for activation of most Nif operons which are directly involved in nitrogen fixation. NifA interacts with sigma-54.; Specificity unclear; 3',5'-cyclic-nucleotide phosphodiesterase 625210 4608585 azo0595 Azoarcus sp. BH72 3',5'-cyclic-nucleotide phosphodiesterase YP_932099.1 624557 D 62928 CDS YP_932100.1 119896887 4606465 625290..626201 1 NC_008702.1 Tyrosine recombinase xerC. Site-specific tyrosine recombinase which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from xerD binding sites by a short central region forming the heterotetrameric xerC-xerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids at ColE1 xer (or cer) and pSC101 (or psi) sites. In the complex xerC specifically exchanges the top DNA strands (By similarity).; High confidence in function and specificity; site-specific recombinase 626201 xerC 4606465 xerC Azoarcus sp. BH72 site-specific recombinase YP_932100.1 625290 D 62928 CDS YP_932101.1 119896888 4610127 626345..626848 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3954 of Azoarcus sp. EbN1 of 56% (gnl|keqq|eba:ebA3954(KEGG)). No domains predictied. No signal peptide. No TMHs. helix-turn-helix present.; hypothetical protein 626848 4610127 azo0597 Azoarcus sp. BH72 hypothetical protein YP_932101.1 626345 D 62928 CDS YP_932102.1 119896889 4606466 626914..628101 1 NC_008702.1 Hypothetical protein yccW TREMBL:Q8Y3C9: 52% identity, 62% similarity. InterPro; IPR002478; PUA. InterPro; IPR000051; SAM_bind. Pfam:Met_1:Met-10+ like-protein SMART; SM00359 TIGR00095: conserved hypothetical protein No transmembrane helices; High confidence in function and specificity; SAM dependent methyl transferase 628101 4606466 azo0598 Azoarcus sp. BH72 SAM dependent methyl transferase YP_932102.1 626914 D 62928 CDS YP_932103.1 119896890 4606467 complement(628098..628448) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA3957 of Azoarcus sp. EbN1 of 38% (gnl|keqq|eba:ebA3957(KEGG)). No domains predicted. signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 628448 4606467 azo0599 Azoarcus sp. BH72 hypothetical protein YP_932103.1 628098 R 62928 CDS YP_932104.1 119896891 4606468 complement(628448..628666) 1 NC_008702.1 Hypothetical secreted protein. no homology of the entire protein to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 628666 4606468 azo0600 Azoarcus sp. BH72 hypothetical protein YP_932104.1 628448 R 62928 CDS YP_932105.1 119896892 4606469 complement(628671..629729) 1 NC_008702.1 promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration; Holliday junction DNA helicase RuvB 629729 ruvB 4606469 ruvB Azoarcus sp. BH72 Holliday junction DNA helicase RuvB YP_932105.1 628671 R 62928 CDS YP_932106.1 119896893 4609836 complement(629785..630384) 1 NC_008702.1 plays an essential role in ATP-dependent branch migration of the Holliday junction; Holliday junction DNA helicase RuvA 630384 ruvA 4609836 ruvA Azoarcus sp. BH72 Holliday junction DNA helicase RuvA YP_932106.1 629785 R 62928 CDS YP_932107.1 119896894 4609835 630563..631267 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BP3397 of B. pertussis of 41% (bpe:BP3397(KEGG)). Tigrfam: azlC: azlC protein (azlC). 6 TMHs no signal peptide; Conserved hypothetical protein; hypothetical protein 631267 4609835 azo0603 Azoarcus sp. BH72 hypothetical protein YP_932107.1 630563 D 62928 CDS YP_932108.1 119896895 4606470 631264..631590 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS04896 of Ralstonia solanacearum o 43% (trembl|Q8Y1W8) Has PF06063, Domain of unknown function (DUF931);IPR010337;Family of transmembrane proteins with undetermined function. singal peptide. 2 THMs; Conserved hypothetical protein; hypothetical protein 631590 4606470 azo0604 Azoarcus sp. BH72 hypothetical protein YP_932108.1 631264 D 62928 CDS YP_932109.1 119896896 4606471 complement(631597..631851) 1 NC_008702.1 Hypothetical protein. Homology to Daro03002672 of Dechloromonas aromatica of 51% (gi|41723577|ref|ZP_00150487.1|(NBCI ENTREZ)). Has PF04023, FeoA domain;IPR007167; This family includes FeoA a small protein, probably involved in Fe2+ transport. This presumed short domain is also found at the C-terminus of a variety of metal dependent transcriptional regulators. This suggests that this domain may be metal-binding. In most cases this is likely to be either iron or manganese. no signal peptide. no TMHs; hypothetical protein 631851 4606471 azo0605 Azoarcus sp. BH72 hypothetical protein YP_932109.1 631597 R 62928 CDS YP_932110.1 119896897 4606472 complement(631860..633851) 1 NC_008702.1 Ferrous iron transport protein B, FeoB.Probable GTP-driven transporter of ferrous ion.Invovled in iron II uptake. 29% FeoB.IPR005289; GTP-bindding_dom.IPR005225; Small_GTP. Pfam:PF02421; FeoB; 1.TIGRFAMs TIGR00437; feoB; 1.TIGR00650; MG442; 1. TIGR00231; small_GTP; 1. TMHelix:12 Siganl pepetide present.; High confidence in function and specificity; putative ferrous iron transport protein B 633851 feoB 4606472 feoB Azoarcus sp. BH72 putative ferrous iron transport protein B YP_932110.1 631860 R 62928 CDS YP_932111.1 119896898 4608756 634049..636247 1 NC_008702.1 Outer membrane TonB-dependent, hydroxamate-type ferrisiderophore receptor. Probably involved in iron transport. 30% TONB_C.IPR000531; TonB_receptor.IPR010105; TonB_siderophor. InterPro: TonB-dependent receptor protein Pfam:PF00593; TonB_dep_Rec; 1. TIGRFAMs:TIGR01783; TonB-siderophor; 1. Signal peptide present.; High confidence in function and specificity; putative TonB-dependent receptor 636247 4608756 azo0607 Azoarcus sp. BH72 putative TonB-dependent receptor YP_932111.1 634049 D 62928 CDS YP_932112.1 119896899 4606473 636324..637007 1 NC_008702.1 Putative PKHD-type hydroxylase, 69% Identity(82% simialrity) to SwissProt;P59727,Q8FJM69, (48% identity) Has SMART;SM00702, P4Hc,Prolyl 4-hydroxylase alpha subunit homologues: IPR006620; Pro_4_hyd_alph; Mammalian enzymes catalyse hydroxylation of collagen, for example. Prokaryotic enzymes might catalyse hydroxylation of antibiotic peptides. These are 2-oxoglutarate-dependent dioxygenases, requiring 2-oxoglutarate and dioxygen as cosubstrates and ferrous iron as a cofactor. Has PF03171,2OG-Fe(II) oxygenase superfamily;IPR005123;This family contains members of the 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily. This family includes the C-terminal of prolyl 4-hydroxylase alpha subunit. The holoenzyme has the activity catalysing the reaction: Procollagen L-proline + 2-oxoglutarate + O2 <=> procollagen trans- 4-hydroxy-L-proline + succinate + CO2. The full enzyme consists of a alpha2 beta2 complex with the alpha subunit contributing most of the parts of the active site. The family also includes lysyl hydrolases,isopenicillin synthases and AlkB.; High confidence in function and specificity; putative hydroxylase 637007 ybiX 4606473 ybiX Azoarcus sp. BH72 putative hydroxylase YP_932112.1 636324 D 62928 CDS YP_932113.1 119896900 4610150 637000..638121 1 NC_008702.1 Probable (S)-2-hydroxy-acid oxidase. Homology to gox of S. oleracea (spinach) of 42% (sprot|GOX_SPIOL). CATALYTIC ACTIVITY: (S)-2-hydroxy-acid + O(2) = 2-oxo acid + H(2)O(2). InterPro: FMN-dependent alpha-hydroxy acid dehydrogenases (IPR000262); Protein binding FMN and related compounds core region (IPR003009) Pfam: FMN-dependent dehydrogenase no signal peptide no TMHs guaA_Cterm: GMP synthase C-terminal d; Family membership; (S)-2-hydroxy-acid oxidase 638121 gox 4610150 gox Azoarcus sp. BH72 (S)-2-hydroxy-acid oxidase YP_932113.1 637000 D 62928 CDS YP_932114.1 119896901 4608931 complement(638132..639553) 1 NC_008702.1 Putative outer membrane efflux protein. Homology to orpM in P. aeruginosa of 36%. Component of an efflux system that confers multidrug or multible antibiotic resistence. Interpro: Outer membran efflux protein Pfam: Outer membran efflux protein signal peptide no TMHs; Family membership; putative outer membrane efflux protein 639553 oprM2 4608931 oprM2 Azoarcus sp. BH72 putative outer membrane efflux protein YP_932114.1 638132 R 62928 CDS YP_932115.1 119896902 4609454 complement(639568..641538) 1 NC_008702.1 Putative efflux transporter for macrolide antibiotics, TREMBL:Q92NU9 (57% identity); TREMBL:Q884D4 (49% identity). Pfam (PF00005): ABC transporter. Pfam (DUF214): Predicted permease. TMHMM reporting four transmembrane helices. TC (3.A.1.122): The Macrolide Exporter Family.; Specificity unclear; ABC transporter ATP-binding protein/permease 641538 macB 4609454 macB Azoarcus sp. BH72 ABC transporter ATP-binding protein/permease YP_932115.1 639568 R 62928 CDS YP_932116.1 119896903 4609191 complement(641535..642686) 1 NC_008702.1 Macrolide-specific efflux protein macA precursor. Efflux transporter for macrolide antibiotics (By similarity). Membrane Fusion Protein (MFP) family.; High confidence in function and specificity; periplasmic protein of efflux system 642686 macA 4609191 macA Azoarcus sp. BH72 periplasmic protein of efflux system YP_932116.1 641535 R 62928 CDS YP_932117.1 119896904 4609190 complement(642997..643413) 1 NC_008702.1 Probable fiopolymer transport protein ExbD. Homology to exbD1 of X. campestris of 43%. ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; High confidence in function and specificity; biopolymer transport protein ExbD 643413 exbD3 4609190 exbD3 Azoarcus sp. BH72 biopolymer transport protein ExbD YP_932117.1 642997 R 62928 CDS YP_932118.1 119896905 4608702 complement(643413..644174) 1 NC_008702.1 Probable biopolymer transport protein ExbB. Homology to exbB of B. pertussis of 50% ExbB is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: MotA/TolQ/ExbB proton channel family Pfam: MotA/TolQ/ExbB proton channel family no signal peptide no TMHs; Family membership; biopolymer transport protein ExbB 644174 exbB2 4608702 exbB2 Azoarcus sp. BH72 biopolymer transport protein ExbB YP_932118.1 643413 R 62928 CDS YP_932119.1 119896906 4608697 complement(644177..644926) 1 NC_008702.1 Putative TonB protein. Homology to tonb2 of P. aeruginosa of 39% (TREMBL:Q9RMT3). To complete transport of bound iron across the inner membrane, a second receptor complex is needed. The major component of this is tonB, a 27kDa protein that facilitates energy transfer from the proton motive force to outer receptors Pfam: Gram-negative bacterial TonB protein no signal peptide 1 TMH; Function unclear; putative TonB protein 644926 tonB1 4608697 tonB1 Azoarcus sp. BH72 putative TonB protein YP_932119.1 644177 R 62928 CDS YP_932120.1 119896907 4609962 complement(645213..645689) 1 NC_008702.1 Bacterioferritin (BFR)(Cytochrome B-1)(Cytochrome B-557).May perform analogous functions in iron detoxification and storage to that of animal ferritins.Key role in iron homeostasis. 71% Bacterioferritin.IPR008331; Ferritin_Dps.IPR009040; Ferritin_like. Pfam:PF00210; ferritin; 1. TIGRFAMs:TIGR00754; bfr; 1.; High confidence in function and specificity; putative bacterioferritin 645689 bfr2 4609962 bfr2 Azoarcus sp. BH72 putative bacterioferritin YP_932120.1 645213 R 62928 CDS YP_932121.1 119896908 4608387 complement(645686..645928) 1 NC_008702.1 Bacterioferritin-associated ferredoxin. Homology to psto4159 of P. syringae of 33% (trembl|Q87XL9). SEEMS TO ASSOCIATE WITH BFR; COULD BE A GENERAL REDOX AND/OR REGULATORY COMPONENT PARTICIPATING IN THE IRON STORAGE MOBILIZATION FUNCTIONS OF BFR. COULD PARTICIPATE IN THE RELEASE OR THE DELIVERY OF IRON FROM/TO BACTERIOFERRITIN (OR OTHER IRON COMPLEXES). Pfam: BFD-like [2Fe-2S] binding domain no signal peptide no TMHs; Conserved hypothetical protein; bacterioferritin-associated ferredoxin 645928 bfd 4608387 bfd Azoarcus sp. BH72 bacterioferritin-associated ferredoxin YP_932121.1 645686 R 62928 CDS YP_932122.1 119896909 4608385 complement(645969..646178) 1 NC_008702.1 47% DUF1008. Pfam:PF06228; DUF1008; 1.; High confidence in function and specificity; putative hemin uptake protein 646178 4608385 azo0618 Azoarcus sp. BH72 putative hemin uptake protein YP_932122.1 645969 R 62928 CDS YP_932123.1 119896910 4606474 646348..647070 1 NC_008702.1 Similar to TREMBL:Q8Y2F3 (57% identity); TREMBL:Q7VVV4 (55% identity); TREMBL:Q9I111 (53% identity). Pfam (FAA_hydrolase): Fumarylacetoacetate (FAA) hydrolase family.; hypothetical protein 647070 4606474 azo0619 Azoarcus sp. BH72 hypothetical protein YP_932123.1 646348 D 62928 CDS YP_932124.1 119896911 4606475 complement(647080..647529) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to an orf of G. sulfurreducens of 34% (tremblnew|AAR35905(SRS)). No domains predicted. Signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 647529 4606475 azo0620 Azoarcus sp. BH72 hypothetical protein YP_932124.1 647080 R 62928 CDS YP_932125.1 119896912 4606476 complement(647516..648724) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA1833 of Azoarcus sp. EbN1 of 48% (gnl|keqq|eba:ebA1833(KEGG)). No domains predicted. Signal peptide Present. No TMH present.; Conserved hypothetical protein; hypothetical protein 648724 4606476 azo0621 Azoarcus sp. BH72 hypothetical protein YP_932125.1 647516 R 62928 CDS YP_932126.1 119896913 4606477 complement(648721..651168) 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to ebA1830 of Azoarcus sp. EbN1 of 63% (gnl|keqq|eba:ebA1830(KEGG)). Pfam: PF00990 GGDEF domain. PF00989 PAS domain. PF00785 PAC motif. PF00563 EAL domain. PF00672 HAMP. TIGRFAM:TIGR00229 PAS domain S-box. TIGR00254 putative diguanylate cyclase (GGDEF) domain. TMHMM reporting 2 transmembrane helices of which one is in the signal peptide. Signal peptide present.; Conserved hypothetical protein; putative signaling protein 651168 4606477 azo0622 Azoarcus sp. BH72 putative signaling protein YP_932126.1 648721 R 62928 CDS YP_932127.1 119896914 4606478 complement(651269..652651) 1 NC_008702.1 Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. 40% Malonyl_CoA_deC. Pfam:PF05292; MCD; 1.; High confidence in function and specificity; putative malonyl-CoA decarboxylase 652651 matA 4606478 matA Azoarcus sp. BH72 putative malonyl-CoA decarboxylase YP_932127.1 651269 R 62928 CDS YP_932128.1 119896915 4609195 652780..653454 1 NC_008702.1 PAS/PAC-domain containing protein, shows good similarity only to parts of other proteins (mostly sensor kinases). This suggests that there normally should be a kinase domain and therefore the protein is not complete. Also the downstream response regulator gives a hint for this suggestion. InterPro: IPR000014 PAS_domain. IPR001610 PAC motif. TIGRFAM: TIGR00229 PAS domain S-box. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; PAS/PAC domain-containing protein 653454 4609195 azo0624 Azoarcus sp. BH72 PAS/PAC domain-containing protein YP_932128.1 652780 D 62928 CDS YP_932129.1 119896916 4606479 653521..653943 1 NC_008702.1 Hypothetical two-component system response regulator, very low similarity to a part of YehT: SWISSPROT: sprot|YEHT_ECOLI (16% Escherichia coli, YehT) Pfam: PF00072 Response_reg.; two-component system response regulator 653943 4606479 azo0625 Azoarcus sp. BH72 two-component system response regulator YP_932129.1 653521 D 62928 CDS YP_932130.1 119896917 4606480 complement(653963..654760) 1 NC_008702.1 transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein; prolipoprotein diacylglyceryl transferase 654760 lgt 4606480 lgt Azoarcus sp. BH72 prolipoprotein diacylglyceryl transferase YP_932130.1 653963 R 62928 CDS YP_932131.1 119896918 4609135 complement(654774..655454) 1 NC_008702.1 Conserved hypothetical protein, 47% identity (59% similarity)to TrEMBL;Q8FIR0.TrEMBL;Q83LI7 Has PF04337,Protein of unknown function, DUF480;IPR007432;This family consists of several proteins of uncharacterised function. Coils2 program reporting presence of Coiled-Coil.; High confidence in function and specificity; hypothetical protein 655454 yceH 4609135 yceH Azoarcus sp. BH72 hypothetical protein YP_932131.1 654774 R 62928 CDS YP_932132.1 119896919 4610157 655581..657689 1 NC_008702.1 Putative adenylate/guanylate cyclase,; Conserved hypothetical protein; putative adenylate/guanylate cyclase 657689 4610157 azo0628 Azoarcus sp. BH72 putative adenylate/guanylate cyclase YP_932132.1 655581 D 62928 CDS YP_932133.1 119896920 4606481 complement(657725..658321) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03000403 of Dechloromonas aromatica of 37% (gi|46140621|ref|ZP_00152222.2|(NBCI ENTREZ)). InterPro: Bacterial outer membrane protein (IPR006664). Pfam: OmpA family. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 658321 4606481 azo0629 Azoarcus sp. BH72 hypothetical protein YP_932133.1 657725 R 62928 CDS YP_932134.1 119896921 4606482 complement(658318..658761) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PJS6w01004783 of Polaromonas sp. JS666 of 45% (gi|54028512|ref|ZP_00360661.1|(NBCI ENTREZ)). No domains predicted. signal peptide. TMH in signal peptide.; Conserved hypothetical protein; hypothetical protein 658761 4606482 azo0630 Azoarcus sp. BH72 hypothetical protein YP_932134.1 658318 R 62928 CDS YP_932135.1 119896922 4606483 658869..661868 1 NC_008702.1 HD-domain containing protein; HD-domain-containing protein 661868 4606483 azo0631 Azoarcus sp. BH72 HD-domain-containing protein YP_932135.1 658869 D 62928 CDS YP_932136.1 119896923 4606484 661937..663787 1 NC_008702.1 catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis; dihydroxy-acid dehydratase 663787 ilvD 4606484 ilvD Azoarcus sp. BH72 dihydroxy-acid dehydratase YP_932136.1 661937 D 62928 CDS YP_932137.1 119896924 4609071 663873..664067 1 NC_008702.1 Conserved hypothetical protein. Homology to Mflag03001107 of Methylobacillus flagellatus of 42% (gi|53759956|ref|ZP_00350342.1|(NBCI ENTREZ)). No Signal peptide or TMH present. No domains predicted.; hypothetical protein 664067 4609071 azo0633 Azoarcus sp. BH72 hypothetical protein YP_932137.1 663873 D 62928 CDS YP_932138.1 119896925 4606485 664076..664372 1 NC_008702.1 Conserved hypothetical protein. Homology to NE1890 of N.europaea of 35% (tremble:Q82TJ7) Has PF05164, Family of unknown function (DUF710);IPR007838;Family of eubacterial hypothetical proteins. No signal peptid. No TMHs; hypothetical protein 664372 4606485 azo0634 Azoarcus sp. BH72 hypothetical protein YP_932138.1 664076 D 62928 CDS YP_932139.1 119896926 4606486 664722..665201 1 NC_008702.1 Hypothetical protein, 72% identity(81% similarity) to TrEMBL;Q82TJ8. TrEMBL;Q8XWR7(62% identity). Has PF04543, Family of unknown function (DUF589);IPR007628 ;Family of uncharacterised proteins; Function unclear; hypothetical protein 665201 4606486 azo0635 Azoarcus sp. BH72 hypothetical protein YP_932139.1 664722 D 62928 CDS YP_932140.1 119896927 4606487 665191..665997 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q82TJ9: 43% identity, 59% similarity InterPro:IPR002781; DUF81. Pfam:PF01925; DUF81 no signal peptide. 8 TMHs; Conserved hypothetical protein; hypothetical protein 665997 4606487 azo0636 Azoarcus sp. BH72 hypothetical protein YP_932140.1 665191 D 62928 CDS YP_932141.1 119896928 4606488 666063..666431 1 NC_008702.1 Hypothetical protein Extremely poor homology with hits in the DB. NO features/signal Peptide/Domains present.; hypothetical protein 666431 4606488 azo0637 Azoarcus sp. BH72 hypothetical protein YP_932141.1 666063 D 62928 CDS YP_932142.1 119896929 4606489 666529..667926 1 NC_008702.1 Conserved hypothtecial protein. Homology to Avin02002811 of Azotobacter vinelandii of 50% (gi|23103464|ref|ZP_00089946.1|(NBCI ENTREZ)). InterPro: Domain of unknown function DUF224 (IPR004017). no signal peptide. no TMHs; hypothetical protein 667926 4606489 azo0638 Azoarcus sp. BH72 hypothetical protein YP_932142.1 666529 D 62928 CDS YP_932143.1 119896930 4606490 668039..668347 1 NC_008702.1 Hypothetical protein. 22% identity to SwissProt;P45905 No domains, repeats, motifs or features present.; hypothetical protein 668347 4606490 azo0639 Azoarcus sp. BH72 hypothetical protein YP_932143.1 668039 D 62928 CDS YP_932144.1 119896931 4606491 complement(668355..669284) 1 NC_008702.1 Part of the ABC transporter complex mntABC involved in manganese uptake. 41% Similar to the putative periplasmic-binding protein MntC precursor in E.coli. The MntC protein is also involved in the resistance to oxidative stress in N gonorrhoaeae. TREMBL E.coli:Q9F4F6. InterPro:IPR006128; Lipoprotein_4.IPR006127; SBP_bac_9. Pfam:PF01297; SBP_bac_9; 1. Signal peptide present.TMHelix:1 This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system periplasmic-binding protein 669284 mntC1 4606491 mntC1 Azoarcus sp. BH72 putative manganese transport system periplasmic-binding protein YP_932144.1 668355 R 62928 CDS YP_932145.1 119896932 4609239 complement(669287..670171) 1 NC_008702.1 Part of the ABC transporter complex mntABC involved in manganese import. Probably responsible for the translocation of the substrate across the membrane. Similar to the permease, MntB in Synechocystis 6803. 30% IPR001626; ABC_transpt3. Pfam; PF00950; ABC-3; 1. This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system permease 670171 mntB1 4609239 mntB1 Azoarcus sp. BH72 putative manganese transport system permease YP_932145.1 669287 R 62928 CDS YP_932146.1 119896933 4609236 complement(670168..670938) 1 NC_008702.1 Manganese transport system ATP-binding protein mntA. This protein is probably a component of a manganese permease a binding protein-dependent ATP-driven transport system (mntABC). Probably responsible for energy coupling to the transport system. 36% AAA_ATPase.IPR003439,AAA ATPase superfamily; ABC_transporter. Pfam: PF00005; ABC_tran; 1. This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system ATP-asa protein 670938 mntA1 4609236 mntA1 Azoarcus sp. BH72 putative manganese transport system ATP-asa protein YP_932146.1 670168 R 62928 CDS YP_932147.1 119896934 4609234 complement(670947..671174) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA1811 of Azoarcus sp. EbN1 of 38% (gi|56476415|ref|YP_158004.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 671174 4609234 azo0643 Azoarcus sp. BH72 hypothetical protein YP_932147.1 670947 R 62928 CDS YP_932148.1 119896935 4606492 complement(671152..671676) 1 NC_008702.1 Putative regulator protein, probably involved in the regulation of cation uptake systems. 32% FUR.IPR009058; Wing_hlx_DNA_bnd. Pfam:PF01475; FUR; 1.Fur family protein 30%; Function unclear; putative regulatory protein 671676 4606492 azo0644 Azoarcus sp. BH72 putative regulatory protein YP_932148.1 671152 R 62928 CDS YP_932149.1 119896936 4606493 complement(671714..672076) 1 NC_008702.1 Conserved hypothetical protein. Homology to PSPTO4639 of P.syringae of 41% (tremble:Q87WB4) No domains predicted. No TMHs. No signal peptide; hypothetical protein 672076 4606493 azo0645 Azoarcus sp. BH72 hypothetical protein YP_932149.1 671714 R 62928 CDS YP_932150.1 119896937 4606494 672312..674498 1 NC_008702.1 HlyB-family protein. Similar to TREMBL:Q7WBM7 (50% identity); TREMBL:Q9KKL9 (41% identity); SWISSPROT:P18770 (28% identity). Pfam (PF00005): ABC transporter. Pfam (PF00664): ABC transporter transmembrane region. TMHMM reporting four transmembrane helices. TC (3.A.1.109.3): LapA adhesin protein exporter, LapB.; High confidence in function and specificity; toxin secretion ABC transporter permease and ATP-binding protein 674498 4606494 azo0646 Azoarcus sp. BH72 toxin secretion ABC transporter permease and ATP-binding protein YP_932150.1 672312 D 62928 CDS YP_932151.1 119896938 4606495 674498..675913 1 NC_008702.1 Putative HylD family secretion protein. Homology to hylD of E. coli of 24% (sprot|HLY4_ECOLI) Involved in the transport of hemolysin A. InterPro: HlyD family secretion protein; Gram-negative bacteril RTX secretion protein D Pfam: HylD family secretion protein no signal peptide no TMHs; Family membership; HlyD family secretion protein 675913 hylD 4606495 hylD Azoarcus sp. BH72 HlyD family secretion protein YP_932151.1 674498 D 62928 CDS YP_932152.1 119896939 4609057 675910..676521 1 NC_008702.1 Putative DNA-binding response regulator, LuxR family,; Function unclear; LuxR family transcriptional regulator 676521 4609057 azo0648 Azoarcus sp. BH72 LuxR family transcriptional regulator YP_932152.1 675910 D 62928 CDS YP_932153.1 119896940 4606496 676646..679489 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA1795 of Azoarcus sp. EbN1 of 46% (gnl|keqq|eba:ebA1795(KEGG)). No domains predicted. No TMHs.; hypothetical protein 679489 4606496 azo0649 Azoarcus sp. BH72 hypothetical protein YP_932153.1 676646 D 62928 CDS YP_932154.1 119896941 4606497 679569..680795 1 NC_008702.1 Conserved hypothetical glycosyltransferase. Homology to gsu0991 of G. sulfurreducens of 30% (tremblnew|AAR34318) Pfam: Glycosyl transferase group 1 no signal peptide no TMHs; Conserved hypothetical protein; glycosyltransferase 680795 4606497 azo0650 Azoarcus sp. BH72 glycosyltransferase YP_932154.1 679569 D 62928 CDS YP_932155.1 119896942 4606498 680822..681160 1 NC_008702.1 Conserved hypothetical protein. Homology to ebB52 Azoarcus sp. EbN1 of 40% (gnl|keqq|eba:ebB52(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 681160 4606498 azo0651 Azoarcus sp. BH72 hypothetical protein YP_932155.1 680822 D 62928 CDS YP_932156.1 119896943 4606499 complement(681161..683074) 1 NC_008702.1 Conserved hypothetical GGDF domain protein. Homology to CV0310 of Chromobacterium violaceum of 34% (trembl|Q7P1A3). Has IPR003660_HAMP,SMART;SM00304:This domain is known as the HAMP domain for histidine kinases,adenylyl cyclases, methyl binding proteins and phosphatases. It is found in bacterial sensor and chemotaxis proteins and in eukaryotic histidine kinases. The bacterial proteins are usually integral membrane proteins and part of a two-component signal transduction pathway. Has IPR000160_GGDEF(SMART;SM00267);This domain is found linked to a wide range of non-homologous domains in a variety of bacteria. The function of this domain is unknown, however it has been shown to be homologous to the adenylyl cyclase catalytic domain. This prediction correlates with the functional information available on two GGDEF-containing proteins, namely diguanylate cyclase and phosphodiesterase A of Acetobacter xylinum, both of which regulate the turnover of cyclic diguanosine monophosphate. Has DUF2: This domain is found in diverse bacterial signaling proteins. It is called EAL after its conserved residues. The EAL domain is a good candidate for a diguanylate phosphodiesterase function. The domain contains many conserved acidic residues that could participate in metal binding and might form the phosphodiesterase active site. It often but not always occurs along with PAS IPR000014 and DUF9 IPR000160 domains that are also found in many signalling proteins. signal peptide 1 TMH; Conserved hypothetical protein; hypothetical protein 683074 4606499 azo0652 Azoarcus sp. BH72 hypothetical protein YP_932156.1 681161 R 62928 CDS YP_932157.1 119896944 4606500 complement(683109..683831) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BB1185 of Bordetella bronchiseptica of 49% (trembl|Q7WN55(SRS)) Signal peptide present. No TMHs Has PF06035, Bacterial protein of unknown function (DUF920);IPR010319; This family consists of several hypothetical bacterial proteins of unknown function.; Conserved hypothetical protein; hypothetical protein 683831 4606500 azo0653 Azoarcus sp. BH72 hypothetical protein YP_932157.1 683109 R 62928 CDS YP_932158.1 119896945 4606501 684035..685477 1 NC_008702.1 Putative outer membrane efflux protein. Homology to aggA from P. putida of 35%. The OEP family (outer membrane efflux protein) allow export of a variety of substrates in Gram negative bacteria. InterPro: Outer membrane efflux protein Pfam: Outer membrane efflux protein signal peptide no TMHs; Function unclear; putative outer membrane efflux protein 685477 aggA 4606501 aggA Azoarcus sp. BH72 putative outer membrane efflux protein YP_932158.1 684035 D 62928 CDS YP_932159.1 119896946 4608286 685558..689061 1 NC_008702.1 Putative two-component sensor histidine kinase. TrEMBL; Q87XU6( 47% identity, 64% similarity),Q6F9J5(43% identity,61% similarity),Q6NAD6(44% Identity,61% similarity). TMHMM2 reporting 2 TMH's present. No Signal peptide Present. Has PF02133;Permease for cytosine/purines, uracil, thiamine, allantoin: IPR001248;Cyt_pur_permease:The Nucleobase Cation Symporter-1 (NCS1) family consists of bacterial and yeast transporters for nucleobases including purines and pyrimidines. Members of this family possess twelve putative transmembrane a-helical spanners (TMSs). At least some of them have been shown to function in uptake by substrate:H+ symport mechanism. Has PF07578:Lipid A Biosynthesis N-terminal domain;This family is found at the N-terminus of a group of Chlamydial Lipid A biosynthesis proteins. It is also found by itself in a family of proteins of unknown function. Has SMART;SM00388:(IPR003661)Dimerisation and phosphoacceptor domain of histidine kinases.The histidine kinase A (phosphoacceptor) N-terminal domain is a dimerisation and phosphoacceptor domain of histidine kinases. It has been found in bacterial sensor protein/histidine kinases. IPR003594;ATPbind_ATPase;(SM00387)This domain is found in several ATP-binding proteins for example: histidine kinase, DNA gyrase B, topoisomerases, heat shock protein HSP90, phytochrome-like ATPases and DNA mismatch repair proteins. SM00448;IPR001789;Response_reg:This domain receives the signal from the sensor partner in bacterial two-component systems. It is usually found N-terminal to a DNA binding effector domain.; High confidence in function and specificity; putative two-component sensor histidine kinase 689061 4608286 azo0655 Azoarcus sp. BH72 putative two-component sensor histidine kinase YP_932159.1 685558 D 62928 CDS YP_932160.1 119896947 4606502 689058..690014 1 NC_008702.1 Putative transcriptional regulator, LuxR family,Response_reg. IPR000792; HTH_LuxR. Pfam: PF00072; response_reg. PF00196; GerE. SMART: SM00448; REC. SM00421; HTH_LUXR. HTH reporting reporting nucleic acid binding motif.; Family membership; LuxR family transcriptional regulator 690014 4606502 azo0656 Azoarcus sp. BH72 LuxR family transcriptional regulator YP_932160.1 689058 D 62928 CDS YP_932161.1 119896948 4606503 691405..692367 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Raeut03005995 of Ralstonia eutropha of 34% (gi|53760601|ref|ZP_00350538.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. 10 TMHs; Conserved hypothetical protein; hypothetical protein 692367 4606503 azo0657 Azoarcus sp. BH72 hypothetical protein YP_932161.1 691405 D 62928 CDS YP_932162.1 119896949 4606504 692364..693995 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein to the data bank. No domains predicted. No signal peptide. 12 TMHs; hypothetical protein 693995 4606504 azo0658 Azoarcus sp. BH72 hypothetical protein YP_932162.1 692364 D 62928 CDS YP_932163.1 119896950 4606505 complement(694005..695342) 1 NC_008702.1 Probable glutathione-disulfide reductase. Homology to gor of E. coli of 53% (sprot|GSHR_ECOLI). Maintain high levels of reduced glutathione in the cytosol. InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327); NAD binding site (IPR000205); Pyridine nuclotide-disulfide oxidoreductase dimerisation doamin (IPR004099); Pyridine nucleotide-disulphide oxidoreductase, class I (IPR001100) Pfam: Pyridine nuccleotide-dusulfphide oxidoreductase; Pyridine nucleotide-disulphide oxidoreductase dimerisation domain no signal peptide no TMHs; High confidence in function and specificity; glutathione-disulfide reductase 695342 gor 4606505 gor Azoarcus sp. BH72 glutathione-disulfide reductase YP_932163.1 694005 R 62928 CDS YP_932164.1 119896951 4608930 695491..697395 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs. Similar to TREMBL:Q87G83 (52% identity); TREMBL:Q9K1A3 (61% identity); SWISSPROT:Q57180 (60% identity). Pfam (PF00005): ABC transporter. TMHMM reporting five transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Specificity unclear; ABC transporter permease and ATP-binding protein 697395 4608930 azo0660 Azoarcus sp. BH72 ABC transporter permease and ATP-binding protein YP_932164.1 695491 D 62928 CDS YP_932165.1 119896952 4606506 697403..697951 1 NC_008702.1 Hypothetical protein ygjP. TREMBL:Q83JJ6: 60% identity,74% similarity (probable metal dependent hydrolase) Members of this family are found in some archaebacteria, as well as Helicobacter pylori. The proteins are 190-240 amino acids long, with the C terminus being the most conserved region, containing three conserved histidines InterPro:IPR002725; DUF45. Pfam:PF01863; DUF45 Non secretory protein with probability of signal peptide being 0.243 No transmembrane helices gmhA: phosphoheptose isomerase; High confidence in function and specificity; hypothetical protein 697951 4606506 azo0661 Azoarcus sp. BH72 hypothetical protein YP_932165.1 697403 D 62928 CDS YP_932166.1 119896953 4606507 complement(697992..698543) 1 NC_008702.1 conserved hypothetical secreted protein. Homology to bA4595 of Azoarcus sp. EbN1 of 52% (gnl|keqq|eba:ebA4595(KEGG)) . No domains predicted. No TMHs. Signal Peptide present.; Conserved hypothetical protein; hypothetical protein 698543 4606507 azo0662 Azoarcus sp. BH72 hypothetical protein YP_932166.1 697992 R 62928 CDS YP_932167.1 119896954 4606508 698767..699378 1 NC_008702.1 Catalysis of the reaction: CDP + alcohol = CMP + phosphatidyl alcohol. Entry name Q8PQ18 Primary accession number: Q8PQ18 InterPro IPR000462; CDP-OH_P_trans. Pfam PF01066; CDP-OH_P_transf; 1. Identities = 114/203 (56%),Prediction: Signal peptide Signal peptide probability: 0.988 Number of predicted TMHs: 3; Family membership; CDP-alcohol phosphatidyltransferase 699378 4606508 azo0663 Azoarcus sp. BH72 CDP-alcohol phosphatidyltransferase YP_932167.1 698767 D 62928 CDS YP_932168.1 119896955 4606509 699386..701152 1 NC_008702.1 Similar to TREMBL:Q87TY0 (64% identity); TREMBL:Q8PQ17 (61% identity); SWISSPROT:P76092 (55% identity).; hypothetical protein 701152 4606509 azo0664 Azoarcus sp. BH72 hypothetical protein YP_932168.1 699386 D 62928 CDS YP_932169.1 119896956 4606510 701149..702507 1 NC_008702.1 Entry name:- Q9I0U5 Primary accession number:-Q9I0U5 InterPro:- IPR008934; AcPase_VanPerase. IPR000340; DS_phosphatase. IPR000326; PA_PTPase. Number of predicted TMHs: 8 Prediction: Non-secretory protein Signal peptide probability: 0.061 IPR000387; TYR_phosphatase. Pfam PF00782; DSPc; 1. PF01569; PAP2; 1. Identity:- 58%; hypothetical protein 702507 4606510 azo0665 Azoarcus sp. BH72 hypothetical protein YP_932169.1 701149 D 62928 CDS YP_932170.1 119896957 4606511 702500..702943 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA2538 of Pseudomonas aeruginosa of 36% (trembl|Q9I0U6). No domains predicted. No signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 702943 4606511 azo0666 Azoarcus sp. BH72 hypothetical protein YP_932170.1 702500 D 62928 CDS YP_932171.1 119896958 4606512 702973..703596 1 NC_008702.1 FUNCTION: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the 2 position. This enzyme can utilize either acyl-CoA or acyl-acyl carrier protein as the fatty acyl donor. CATALYTIC ACTIVITY: Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Entry name:-SWISSPROT:PLSC_ECOLI Prim. accession # P26647 InterPro:-IPR002123; Acyltransferase. IPR004552; AGP_acyltrn. Pfam:-PF01553; Acyltransferase; 1. Identities = 42/127 (33%) Prediction: Non-secretory protein Signal peptide probability: 0.014 Number of predicted TMHs: 0; Family membership; putative acyltransferase 703596 4606512 azo0667 Azoarcus sp. BH72 putative acyltransferase YP_932171.1 702973 D 62928 CDS YP_932172.1 119896959 4606513 703602..704546 1 NC_008702.1 Conserved hypothetical phoshpatidate cytidyltransferase. Homology to pa2536 of P. aeruginosa (trembl|Q9I0U8). Phosphatidate cytidylyltransferase is the enzyme that catalyzes the synthesis of CDP-diacylglycerol from CTP and phosphatidate (PA): CTP + phosphatidate = diphosphate + CDP-diacylglycerol Pfam: Phospatidate cytidyltransferase signal peptide 7 TMHs InterPro: Phosphatidate cytidylyltransferase stp: serine transporter; Family membership; phosphatidate cytidylyltransferase 704546 4606513 azo0668 Azoarcus sp. BH72 phosphatidate cytidylyltransferase YP_932172.1 703602 D 62928 CDS YP_932173.1 119896960 4606514 complement(704566..705237) 1 NC_008702.1 Probable cytochrome c-type protein NapC. Homology to napC of r. sphaeroides of 67% (sprot|NAPC_RHOSH). MEDIATES ELECTRON FLOW FROM QUINONES TO THE NAPAB COMPLEX. signal peptide no TMHs; High confidence in function and specificity; cytochrome C-type protein NapC 705237 napC1 4606514 napC1 Azoarcus sp. BH72 cytochrome C-type protein NapC YP_932173.1 704566 R 62928 CDS YP_932174.1 119896961 4609332 complement(705250..705717) 1 NC_008702.1 probable diheme cytochrome c. Homology to napB of A. eutrophus of 51% (sprot|NAPB_ALCEU). SMALL SUBUNIT OF THE PERIPLASMIC NITRATE REDUCTASE (NAP). ONLY EXPRESSED AT HIGH LEVELS DURING AEROBIC GROWTH. NAPAB COMPLEX RECEIVES ELECTRONS FROM THE MEMBRANE-ANCHORED TETRAHEME NAPC PROTEIN THUS ALLOWING ELECTRON FLOW BETWEEN MEMBRANE AND PERIPLASM. ESSENTIAL FUNCTION FOR NITRATE ASSIMILATION AND MAY HAVE A ROLE IN ANAEROBIC METABOLISM. signal peptide no TMHs; High confidence in function and specificity; diheme cytochrome C 705717 napB1 4609332 napB1 Azoarcus sp. BH72 diheme cytochrome C YP_932174.1 705250 R 62928 CDS YP_932175.1 119896962 4609330 complement(705728..708220) 1 NC_008702.1 periplasmic; catalytic subunit; with NapBC catalyzes the reduction of nitrate to nitrite; NapAB receives electrons from NapC; nitrate reductase catalytic subunit 708220 napA1 4609330 napA1 Azoarcus sp. BH72 nitrate reductase catalytic subunit YP_932175.1 705728 R 62928 CDS YP_932176.1 119896963 4609328 complement(708192..708494) 1 NC_008702.1 Putative NapD protein, component of of periplasmic nitrate reductase system. Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme. Homology to napD of Rhodobacter sphaeroides of 26% (gi|3345484|dbj|AB016290.1|). InterPro:IPR005623; NapD. Pfam:PF03927; NapD; 1. no signal peptide. No TMHs.; Function unclear; putative NapD protein 708494 napD1 4609328 napD1 Azoarcus sp. BH72 putative NapD protein YP_932176.1 708192 R 62928 CDS YP_932177.1 119896964 4609334 complement(708506..708664) 1 NC_008702.1 Putative periplasmic nitrate reductase accessory protein NapE. Homology to napE of R. sphaeroides of 36% (trembl|O88159). Pfam: Periplasmic nitrate reductase NapE (PF06796) This family consists of several bacterial periplasmic nitrate reductase NapE proteins. NapE is thought to be a transmembrane protein of the reductase. Interpro: Periplasmic nitrate reductase NapE (IPR010649) no signal peptide 1 TMH; Family membership; putative periplasmic nitrate reductase accessory protein NapE 708664 napE 4609334 napE Azoarcus sp. BH72 putative periplasmic nitrate reductase accessory protein NapE YP_932177.1 708506 R 62928 CDS YP_932178.1 119896965 4609336 709010..710266 1 NC_008702.1 AmiC regulates the expression of the inducible aliphatic amidase activity in Pseudomonas aeruginosa. Similar to trembl|Q87VQ0 (80%) and to sprot|AMIC_PSEAE (27%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative aliphatic amidase expression-regulating protein 710266 amiC2 4609336 amiC2 Azoarcus sp. BH72 putative aliphatic amidase expression-regulating protein YP_932178.1 709010 D 62928 CDS YP_932179.1 119896966 4608309 710535..712166 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q87VP9 (50%)and to pir|AB0146 (48%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting nine Tmhelix. SignalP reporting Signal peptide.; Specificity unclear; ABC transporter permease 712166 4608309 azo0675 Azoarcus sp. BH72 ABC transporter permease YP_932179.1 710535 D 62928 CDS YP_932180.1 119896967 4606515 712360..713553 1 NC_008702.1 Probable ABC transporter membrane spanning protein. Homology to urtC of Anabaena sp. of 41% (involved in urea transport). Part of the binding-protein-dependent transport system. Probably responsible for the translocation of the substrates across the membrane. InterPro: Binding-system dependent bacterial transporters (araH livH/limM families) Pfam: Branched-chain amino acid transport system signal peptide probable 9 TMHs; Function unclear; ABC transporter membrane protein 713553 urtC 4606515 urtC Azoarcus sp. BH72 ABC transporter membrane protein YP_932180.1 712360 D 62928 CDS YP_932181.1 119896968 4610076 713550..714407 1 NC_008702.1 Conserved hypothetical ABC transporter ATP binding protein. Homology to pp4844 of P. putida of 70% (trembl|Q88DI1). Probable component of a branched-chain amino-acid transport system. Pfam: ABC-Transporter no signal peptide no TMHs; Family membership; ABC transporter ATP-binding protein 714407 urtD 4610076 urtD Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932181.1 713550 D 62928 CDS YP_932182.1 119896969 4610077 714834..715526 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q88DI0 (59%) and to sprot|BRAG_PSEAE (35%). Pfam: ABC transporter Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 715526 urtE 4610077 urtE Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932182.1 714834 D 62928 CDS YP_932183.1 119896970 4610078 715560..716513 1 NC_008702.1 Probable nodulation protein,; Function unclear; nodulation protein 716513 nodD 4610078 nodD Azoarcus sp. BH72 nodulation protein YP_932183.1 715560 D 62928 CDS YP_932184.1 119896971 4609389 complement(716527..717309) 1 NC_008702.1 Function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotobetainyl-CoA. Entry name CAID_ECO57 Primary accession number Q8XA35 InterPro IPR001753; EnCoA_hydrtse. Pfam PF00378; ECH; 1. Identities = 30% Prediction: Non-secretory protein Signal peptide probability: 0.003 Number of predicted TMHs: 0; Family membership; putative carnitinyl-CoA dehydratase 717309 caiD 4609389 caiD Azoarcus sp. BH72 putative carnitinyl-CoA dehydratase YP_932184.1 716527 R 62928 CDS YP_932185.1 119896972 4608411 717556..717876 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PP2796 of P. putida of 44% (trembl|Q88J53) PF04341,Protein of unknown function, DUF485;IPR007436;This family includes several putative integral membrane proteins. signal peptide 1 TMHs; Conserved hypothetical protein; hypothetical protein 717876 4608411 azo0681 Azoarcus sp. BH72 hypothetical protein YP_932185.1 717556 D 62928 CDS YP_932186.1 119896973 4606516 717876..719555 1 NC_008702.1 member of the sodium:solute symporter family; cotranscribed with the acs gene which encodes acetyl coenzyme A synthase; mutations affect acetate uptake; acetate permease 719555 actP 4606516 actP Azoarcus sp. BH72 acetate permease YP_932186.1 717876 D 62928 CDS YP_932187.1 119896974 4606517 719709..721004 1 NC_008702.1 Hypothetical protein ytfL. TREMBL:Q7NQ98: 61% identity, 78% similarity InterPro IPR002550; CBS. IPR000644; CBS_domain. IPR005170; CorC_transpt-asc. Pfam: PF00571; CBS; 2. PF03471; CorC_HlyC; PF01595; DUF21; gntP: gluconate transporter Signal peptide present (Signal P predicted) Transmembrane helices 4 (TMHMM predicted); High confidence in function and specificity; hemolysin 721004 ytfL1 4606517 ytfL1 Azoarcus sp. BH72 hemolysin YP_932187.1 719709 D 62928 CDS YP_932188.1 119896975 4610197 complement(721389..722060) 1 NC_008702.1 Transcriptional regulator, GntR-family,; High confidence in function and specificity; GntR family transcriptional regulator 722060 mdcY 4610197 mdcY Azoarcus sp. BH72 GntR family transcriptional regulator YP_932188.1 721389 R 62928 CDS YP_932189.1 119896976 4609205 722387..724552 1 NC_008702.1 MDM; functions in conversion of succinate to propionate; methylmalonyl-CoA mutase 724552 mutB 4609205 mutB Azoarcus sp. BH72 methylmalonyl-CoA mutase YP_932189.1 722387 D 62928 CDS YP_932190.1 119896977 4609295 724619..725632 1 NC_008702.1 functions in transport of arginine/ornithine; inner membrane ATPase that cleaves ATP and phosphorylates two periplasmic proteins that function as two distinct transport systems, the AO (arginine and ornithine) and LAO (lysine, arginine, and ornithine) periplasmic binding proteins; arginine/ornithine transport system ATPase 725632 argK 4609295 argK Azoarcus sp. BH72 arginine/ornithine transport system ATPase YP_932190.1 724619 D 62928 CDS YP_932191.1 119896978 4608339 725698..727233 1 NC_008702.1 Activity:- ATP + propanoyl-CoA + HCO3- = ADP + phosphate + (S)-methylmalonyl-CoA Entry name:-TREMBL:Q8U9Y9 Prim. accession # Q8U9Y9 InterPro:-IPR000438; ACoACC_transB. IPR000022; Carboxyl_trans. Pfam:- PF01039; Carboxyl_trans; 1. Identities = 363/511 (71%) Number of predicted TMHs: 0; Family membership; propionyl-coa carboxylase subunit beta 727233 pccB 4608339 pccB Azoarcus sp. BH72 propionyl-coa carboxylase subunit beta YP_932191.1 725698 D 62928 CDS YP_932192.1 119896979 4609507 727372..729378 1 NC_008702.1 Activity:- ATP + propanoyl-CoA + HCO3- = ADP + phosphate + (S)-methylmalonyl-CoA Entry name :- Q8U9Z4 Primary accession number:- Q8U9Z4 InterPro:- IPR001882; Biotin_BS. IPR005482; Biotin_carb_C. IPR000089; Biotin_lipoyl. IPR005481; CPase_L_N. IPR005479; Cphp_synth_L_D2. Pfam:- PF02785; Biotin_carb_C; 1. PF00364; Biotin_lipoyl; 1. PF00289; CPSase_L_chain; 1. PF02786; CPSase_L_D2; 1. Identities = 400/673 (59%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; propionyl-CoA carboxylase subunit alpha 729378 pccA 4609507 pccA Azoarcus sp. BH72 propionyl-CoA carboxylase subunit alpha YP_932192.1 727372 D 62928 CDS YP_932193.1 119896980 4609506 729561..732017 1 NC_008702.1 GGEF/EAL/PAS/PAC-domain containing protein, sym; pNGR234a) / TREMBL: trembl|Q7NYX4 (40% Chromobacterium violaceum, cv1148) InterPro: IPR000160 GGDEF. IPR000014 PAS. IPR000700 PAS-assoc_C. IPR001633 EAL. Pfam: PF00990 GGDEF domain. PF00989 PAS domain. PF00785 PAC motif. PF00563 EAL domain. TIGRFAM:TIGR00229 PAS domain S-box. TIGR00254 putative diguanylate cyclase (GGDEF) domain.; GGEF/EAL/PAS/PAC-domain-containing protein 732017 4609506 azo0689 Azoarcus sp. BH72 GGEF/EAL/PAS/PAC-domain-containing protein YP_932193.1 729561 D 62928 CDS YP_932194.1 119896981 4606518 732026..733909 1 NC_008702.1 Conserved hypothetical protein. Homology to PA4929 of P.aeruginosa of 32% (trembl|Q9HUN5(SRS)). Signal P reporting presence of signal peptide. 7 TMHs Has PF07696: 7TMR-DISM extracellular 2; This entry represents one of two distinct types of extracellular domain found in the 7TM-DISM (7TM Receptors with Diverse Intracellular Signalling Modules) bacterial transmembrane proteins. It is possible that this domain adopts a jelly roll fold and acts as a receptor for carbohydrates and their derivatives. Has PF07695:7TM diverse intracellular signalling domain;This entry represents the transmembrane region of the 7TM-DISM (7TM Receptors with Diverse Intracellular Signalling Modules). Has IPR000160;GGDEF:(SM00267)This domain is found linked to a wide range of non-homologous domains. The function of this domain is unknown This domain is found in proteins which contain bacterial signaling domains. Has PF07696:7TMR-DISM extracellular 2 domain;This entry represents one of two distinct types of extracellular domain found in the 7TM-DISM (7TM Receptors with Diverse Intracellular Signalling Modules) bacterial transmembrane proteins. It is possible that this domain adopts a jelly roll fold and acts as a receptor for carbohydrates and their derivatives.; hypothetical protein 733909 4606518 azo0690 Azoarcus sp. BH72 hypothetical protein YP_932194.1 732026 D 62928 CDS YP_932195.1 119896982 4606519 734005..734865 1 NC_008702.1 Conserved hypothetical metallopeptidase. Homology to bpp2257 of B. parapertussis of 63% (trembl|Q7W887). Members of this family of bacterial proteins are described as hypothetical proteins or zinc metallopeptidases. The majority have a HExxH zinc-binding motif characteristic of neutral zinc metallopeptidases, however there is no evidence to support their function as metallopeptidases. Pfam: putaive neutral zinc metallopeptidase no signal peptide 1 TMH; Specificity unclear; metallopeptidase 734865 4606519 azo0691 Azoarcus sp. BH72 metallopeptidase YP_932195.1 734005 D 62928 CDS YP_932196.1 119896983 4606520 complement(734887..735363) 1 NC_008702.1 Glyoxalase I (lactoylglutathione lyase) catalyzes the first step of the glyoxal pathway in the following reaction: glutathione + methylglyoxal = (R)-S-lactoylglutathione,; Function unclear; lactoylglutathione lyase 735363 gloA1 4606520 gloA1 Azoarcus sp. BH72 lactoylglutathione lyase YP_932196.1 734887 R 62928 CDS YP_932197.1 119896984 4608907 complement(735949..736680) 1 NC_008702.1 Probable DNA-binding response regulator,Response_reg. IPR001867; Trans_reg_C. Pfam: PF00072; response_reg. PF00486; trans_reg_C. SMART: SM00448; REC.; Specificity unclear; DNA-binding response regulator 736680 virG 4608907 virG Azoarcus sp. BH72 DNA-binding response regulator YP_932197.1 735949 R 62928 CDS YP_932198.1 119896985 4610094 complement(736677..738734) 1 NC_008702.1 Putative hybrid sensor and regulator protein,; Specificity unclear; putative hybrid sensor and regulator protein 738734 4610094 azo0694 Azoarcus sp. BH72 putative hybrid sensor and regulator protein YP_932198.1 736677 R 62928 CDS YP_932199.1 119896986 4606521 complement(738888..739898) 1 NC_008702.1 Putative ornithine utilization regulator,; Specificity unclear; putative ornithine utilization regulator 739898 oruR1 4606521 oruR1 Azoarcus sp. BH72 putative ornithine utilization regulator YP_932199.1 738888 R 62928 CDS YP_932200.1 119896987 4609458 740204..743470 1 NC_008702.1 Methylmalonyl-CoA mutase mitochondrial precursor (EC 5.4.99.2) (MCM). INVOLVED IN MAN IN THE DEGRADATION OF SEVERAL AMINO ACIDS ODD-CHAIN FATTY ACIDS AND CHOLESTEROL VIA PROPIONYL-COA TO THE TRICARBOXYLIC ACID CYCLE. MCM HAS DIFFERENT FUNCTIONS IN OTHER SPECIES. Activity:- (R)-2-Methylmalonyl-CoA = succinyl-CoA Entry name :-TREMBL:Q8Y2U5 Prim. accession # Q8Y2U5 Identities = 797/1103 (72%) InterPro:- IPR006159; Acid_CoA_mut_C. IPR005129; ArgK. IPR006158; B12-binding. IPR006099; MMCoA_mutase. IPR006098; MMCoA_mutase_N. Pfam:- PF03308; ArgK; 1. PF02310; B12-binding; 1. PF01642; MM_CoA_mutase; 1. Prediction: Non-secretory protein Signal peptide probability: 0.005 Number of predicted TMHs: 0; Family membership; methylmalonyl-coa mutase 743470 4609458 azo0696 Azoarcus sp. BH72 methylmalonyl-coa mutase YP_932200.1 740204 D 62928 CDS YP_932201.1 119896988 4606522 complement(743567..743848) 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 743848 4606522 azo0697 Azoarcus sp. BH72 hypothetical protein YP_932201.1 743567 R 62928 CDS YP_932202.1 119896989 4606523 complement(743845..745224) 1 NC_008702.1 class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle; fumarate hydratase 745224 fumC 4606523 fumC Azoarcus sp. BH72 fumarate hydratase YP_932202.1 743845 R 62928 CDS YP_932203.1 119896990 4608846 complement(745304..746044) 1 NC_008702.1 Hypothetical secreted protein. No significant homology to the data bank. no domains pedicted. signal peptide. TMH in signal peptide; hypothetical protein 746044 4608846 azo0699 Azoarcus sp. BH72 hypothetical protein YP_932203.1 745304 R 62928 CDS YP_932204.1 119896991 4606524 complement(746188..747486) 1 NC_008702.1 Hypothetical protein, 52% identity to TrEMBL;Q8XYG9. Has PF03781, Domain of unknown function (DUF323);IPR005532 ;This presumed domain is found in bacterial proteins. In some cases these proteins also contain a protein kinase domain. The function of this domain is unknown. The domain has also been found in a eukaryotic protein, required for post-translational sulphatase modification.; Function unclear; hypothetical protein 747486 4606524 azo0700 Azoarcus sp. BH72 hypothetical protein YP_932204.1 746188 R 62928 CDS YP_932205.1 119896992 4606525 complement(747598..748953) 1 NC_008702.1 (N-acetyl-beta-glucosaminidase)(Beta-N- acetylhexosaminidase). Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides (By similarity). Hydrolyzes rapidly p-nitrophenyl-N-acetyl-beta-D-glucosaminide (PNP-beta-GlcNAc) and 4-methylumbelliferyl-beta-GlcNAc, and slightly active on p-nitrophenyl-beta-GalNAc. May play a role in signal transduction between host and organism. 34% Glyco_hydro_3N. Pfam:PF00933; Glyco_hydro_3; 1. TMhelix:2.; High confidence in function and specificity; putative beta-hexosaminidase 748953 nagZ1 4606525 nagZ1 Azoarcus sp. BH72 putative beta-hexosaminidase YP_932205.1 747598 R 62928 CDS YP_932206.1 119896993 4609315 complement(748950..750281) 1 NC_008702.1 CreD confers tolerance to colicin E2. Similar to SWISSPROT:P08369 (35% identity). TMHMM reporting six transmembrane helices.; High confidence in function and specificity; hypothetical protein 750281 creD 4609315 creD Azoarcus sp. BH72 hypothetical protein YP_932206.1 748950 R 62928 CDS YP_932207.1 119896994 4608532 complement(750394..751824) 1 NC_008702.1 part of a two-component regulatory system with CreB or PhoB; involved in catabolic regulation; sensory histidine kinase CreC 751824 creC 4608532 creC Azoarcus sp. BH72 sensory histidine kinase CreC YP_932207.1 750394 R 62928 CDS YP_932208.1 119896995 4608531 complement(751848..752555) 1 NC_008702.1 response regulator in two-component regulatory system with CreC; CreB protein is phosphorylated by sensor protein phospho-CreC; involved in catabolic regulation; DNA-binding response regulator CreB 752555 creB 4608531 creB Azoarcus sp. BH72 DNA-binding response regulator CreB YP_932208.1 751848 R 62928 CDS YP_932209.1 119896996 4608530 complement(752570..754012) 1 NC_008702.1 Probable magnesium transporter MgtE. Members of this family probably transport Mg2+ or other divalent cations into the cell. 26% CBS_domain.IPR006667; MgtE_integrmembr. Pfam:PF00571; CBS; 2.PF01769; MgtE; 1. TMHleix: 3.; Function unclear; putative magnesium transport protein 754012 mgtE 4608530 mgtE Azoarcus sp. BH72 putative magnesium transport protein YP_932209.1 752570 R 62928 CDS YP_932210.1 119896997 4609216 complement(754167..754862) 1 NC_008702.1 glycosyltransferase; polymerizes glycan strands in the peptidoglycan; monofunctional biosynthetic peptidoglycan transglycosylase 754862 mtgA 4609216 mtgA Azoarcus sp. BH72 monofunctional biosynthetic peptidoglycan transglycosylase YP_932210.1 754167 R 62928 CDS YP_932211.1 119896998 4609282 complement(754859..755704) 1 NC_008702.1 AroE; catalyzes the conversion of shikimate to 3-dehydroshikimate; shikimate 5-dehydrogenase 755704 aroE 4609282 aroE Azoarcus sp. BH72 shikimate 5-dehydrogenase YP_932211.1 754859 R 62928 CDS YP_932212.1 119896999 4608345 complement(755718..756620) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ne1626 of N. europaea of 46% (trembl|Q82U75) no domains predicted signal peptide TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 756620 4608345 azo0708 Azoarcus sp. BH72 hypothetical protein YP_932212.1 755718 R 62928 CDS YP_932213.1 119897000 4606526 complement(756666..758567) 1 NC_008702.1 Putative exoribonuclease II (EC: 3.1.13.1), RNase R / VacB protein) / TREMBL: trembl|Q7P1X0 (50% Chromobacterium violaceum, probable ribonuclease ll,cv0092) InterPro: IPR001900 Ribonuclease_II. Pfam: PF00773 RNB-like protein.; Conserved hypothetical protein; putative exoribonuclease II 758567 4606526 azo0709 Azoarcus sp. BH72 putative exoribonuclease II YP_932213.1 756666 R 62928 CDS YP_932214.1 119897001 4606527 complement(758634..759479) 1 NC_008702.1 Similar to TREMBL:Q8KNG7 (29% identity); TREMBL:Q8UEX8 (27% identity). Pfam (PF01209): ubiE/COQ5 methyltransferase family.; Function unclear; putative methyltransferase 759479 4606527 azo0710 Azoarcus sp. BH72 putative methyltransferase YP_932214.1 758634 R 62928 CDS YP_932215.1 119897002 4606528 complement(759510..760103) 1 NC_008702.1 Conserrved hypothetical protein. Homology to rsc2781 of R. solanacearum of 51% (trembl|Q8XVP9) Pfam: UPF0227 Despite being classed as uncharacterised proteins,the members of this family are almost certainly enzymes that are distantly related to the Abhydrolase_1. no signal peptide no TMHs; hypothetical protein 760103 4606528 azo0711 Azoarcus sp. BH72 hypothetical protein YP_932215.1 759510 R 62928 CDS YP_932216.1 119897003 4606529 complement(760137..760961) 1 NC_008702.1 BacA; phosphatase activity in Escherichia coli not kinase; involved in bacitracin resistance as bacitracin supposedly sequesters undecaprenyl disphosphate which reduces the pool of lipid carrier available to the cell; undecaprenyl pyrophosphate phosphatase 760961 uppP 4606529 uppP Azoarcus sp. BH72 undecaprenyl pyrophosphate phosphatase YP_932216.1 760137 R 62928 CDS YP_932217.1 119897004 4610069 complement(761053..761304) 1 NC_008702.1 Hypothetical protein PA1578.1. TREMBL:Q7W9P0:69% identity, 78% similarity TREMBL: Q8PDJ2: 69% identity, 74% similarity (predicted proteinase inhibitor)) Probable Fe-S cluster bearing oxidoreductase function. InterPro:IPR005358; UPF0153. Pfam:PF03692; UPF0153; High confidence in function and specificity; hypothetical protein 761304 4610069 azo0713 Azoarcus sp. BH72 hypothetical protein YP_932217.1 761053 R 62928 CDS YP_932218.1 119897005 4606530 complement(761309..761842) 1 NC_008702.1 Conserved hypothetical protein. Homology to RPA1001 of R.palustris of 37% (tremble:Q6NB29). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 761842 4606530 azo0714 Azoarcus sp. BH72 hypothetical protein YP_932218.1 761309 R 62928 CDS YP_932219.1 119897006 4606531 complement(761844..762548) 1 NC_008702.1 Conserved hypothetical protein. Homology to DP0001 of Desulfotalea psychrophila of 43% (gnl|keqq|dps:DP0001(KEGG)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 762548 4606531 azo0715 Azoarcus sp. BH72 hypothetical protein YP_932219.1 761844 R 62928 CDS YP_932220.1 119897007 4606532 762691..766773 1 NC_008702.1 ATP-dependent helicase hrpA. Not yet known. InterPro: DEAD/DEAH box helicase; High confidence in function and specificity; putative ATP-dependent helicase 766773 hrpA 4606532 hrpA Azoarcus sp. BH72 putative ATP-dependent helicase YP_932220.1 762691 D 62928 CDS YP_932221.1 119897008 4609029 complement(766798..767121) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA3912 of Azoarcus sp. EbN1 of 45% (gi|56477657|ref|YP_159246.1|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 767121 4609029 azo0717 Azoarcus sp. BH72 hypothetical protein YP_932221.1 766798 R 62928 CDS YP_932222.1 119897009 4606533 767369..767752 1 NC_008702.1 binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21; 30S ribosomal protein S6 767752 rpsF 4606533 rpsF Azoarcus sp. BH72 30S ribosomal protein S6 YP_932222.1 767369 D 62928 CDS YP_932223.1 119897010 4609809 767768..768079 1 NC_008702.1 Putative primosomal replication protein N Homology to priB of E. coli of 31% (sprot|PRIB_ECOLI) Component of the preprimosomal complex composed of priA, priB, priC,dnaB and dnaT. Upon transient interaction with dnaG it forms the primosome. Binds single-stranded DNA. Pfam: Single-stranded binding protein family (PF00436) Interpro: Single stranded binding (IPR010913) no signal peptide no TMHs; Family membership; putative primosomal replication proteinN 768079 priB 4609809 priB Azoarcus sp. BH72 putative primosomal replication proteinN YP_932223.1 767768 D 62928 CDS YP_932224.1 119897011 4609627 768081..768356 1 NC_008702.1 binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 768356 rpsR 4609627 rpsR Azoarcus sp. BH72 30S ribosomal protein S18 YP_932224.1 768081 D 62928 CDS YP_932225.1 119897012 4609821 768375..768830 1 NC_008702.1 in Escherichia coli this protein is wrapped around the base of the L1 stalk; 50S ribosomal protein L9 768830 rplI 4609821 rplI Azoarcus sp. BH72 50S ribosomal protein L9 YP_932225.1 768375 D 62928 CDS YP_932226.1 119897013 4609768 768968..770377 1 NC_008702.1 Replicative DNA helicase (EC 3.6.1.-). PARTICIPATES IN INITIATION AND ELONGATION DURING CHROMOSOME REPLICATION; IT EXHIBITS DNA-DEPENDENT ATPASE ACTIVITY AND CONTAINS DISTINCT ACTIVE SITES FOR ATP BINDING DNA BINDING AND INTERACTION WITH DNAC PROTEIN PRIMASE AND OTHER PREPRIMING PROTEINS. InterPro: DnaB helicase; High confidence in function and specificity; DnaB protein 770377 dnaB 4609768 dnaB Azoarcus sp. BH72 DnaB protein YP_932226.1 768968 D 62928 CDS YP_932227.1 119897014 4608237 776049..777212 1 NC_008702.1 Putative monoxygenase. Pfam: Monoxygenase InterPro: Aromatic-ring hydroxylase (flavoprotein monoxygenase); High confidence in function and specificity; ubiquinone biosynthesis hydroxylase family protein 777212 4608237 azo0723 Azoarcus sp. BH72 ubiquinone biosynthesis hydroxylase family protein YP_932227.1 776049 D 62928 CDS YP_932228.1 119897015 4606534 777226..777951 1 NC_008702.1 Putative protein disulfide-isomerase. Homology to dsbC of E. coli of 29% (sprot|DSBC_ECOLI) REQUIRED FOR DISULFIDE BOND FORMATION IN SOME PERIPLASMIC PROTEINS. ACTS BY TRANSFERRING ITS DISULFIDE BOND TO OTHER PROTEINS AND IS REDUCED IN THE PROCESS. DSBC IS REOXIDIZED BY A YET UNCHARACTERIZED PROTEIN. ALSO ACTS AS A DISULFIDE ISOMERASE. signal peptide no TMHs; Family membership; putative protein disulfide-isomerase 777951 dsbC 4606534 dsbC Azoarcus sp. BH72 putative protein disulfide-isomerase YP_932228.1 777226 D 62928 CDS YP_932229.1 119897016 4608655 complement(777974..779038) 1 NC_008702.1 Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-) (Murein hydrolase A) (Mlt38). MUREIN-DEGRADING ENZYME. MAY PLAY A ROLE IN RECYCLING OF MUROPEPTIDES DURING CELL ELONGATION AND/OR CELL DIVISION. OPTIMAL ACTIVITY IS BETWEEN PH 4.0 AND 4.5; LOSES ITS ACTIVITY RAPIDLY AT TEMPERATURES ABOVE 30 DEGREES CELSIUS. DEGRADES MUREIN GLYCAN STRANDS AND INSOLUBLE HIGH-MOLECULAR WEIGHT MUREIN SACCULI. ssl1: transcription factor ssl1; High confidence in function and specificity; membrane-bound transglycosylase 779038 mltA 4608655 mltA Azoarcus sp. BH72 membrane-bound transglycosylase YP_932229.1 777974 R 62928 CDS YP_932230.1 119897017 4609227 779280..779393 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 779393 4609227 azo0726 Azoarcus sp. BH72 hypothetical protein YP_932230.1 779280 D 62928 CDS YP_932231.1 119897018 4606535 779386..780249 1 NC_008702.1 Conserved hypothetical ATPase family protein. Homology to bb4427 of B. bronchiseptica of 65% (trembl|Q7WF53). Pfam: ATPase family associated with various cellular activities This large family has the key feature that they share a conserved region of about 220 amino acids that contains an ATP-binding site. no signal peptide no TMHs; Conserved hypothetical protein; ATPase family protein 780249 4606535 azo0727 Azoarcus sp. BH72 ATPase family protein YP_932231.1 779386 D 62928 CDS YP_932232.1 119897019 4606536 780242..781198 1 NC_008702.1 NUDIX hydrolase/thiamine phosphate synthase Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) (By similarity). InterPro: NUDIX hydrolase TIGRFAM: mutt: mutator mutT protein; High confidence in function and specificity; hypothetical protein 781198 4606536 azo0728 Azoarcus sp. BH72 hypothetical protein YP_932232.1 780242 D 62928 CDS YP_932233.1 119897020 4606537 complement(781195..781395) 1 NC_008702.1 Conserved hypothetical protein. Homology to RSc2830 of Ralstonia solanacearum of 53% (trembl:Q8XVK0). Has PF03884, Domain of unknown function (DUF329);IPR005584;The function of this short domain is unknown it contains four conserved cysteines and may therefore be involved in zinc binding. no TMHs. No signal peptide.; hypothetical protein 781395 4606537 azo0729 Azoarcus sp. BH72 hypothetical protein YP_932233.1 781195 R 62928 CDS YP_932234.1 119897021 4606538 complement(781398..782153) 1 NC_008702.1 Hypothetical protein, yacF, 32% identitcal to SwissProt;P36680,Q8FL56. Has PF07072, Protein of unknown function (DUF1342);IPR009777 ;This family consists of several hypothetical bacterial proteins of around 250 residues in length. Members of this family are often known as YacF after the Escherichia coli protein P36680. The function of this family is unknown. cobA: cob(I)alamin adenosyltransferase; hypothetical protein 782153 yacF 4606538 yacF Azoarcus sp. BH72 hypothetical protein YP_932234.1 781398 R 62928 CDS YP_932235.1 119897022 4610135 complement(782235..782855) 1 NC_008702.1 catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis; dephospho-CoA kinase 782855 coaE 4610135 coaE Azoarcus sp. BH72 dephospho-CoA kinase YP_932235.1 782235 R 62928 CDS YP_932236.1 119897023 4608496 complement(782852..783562) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rs03277 of R. solanacearum of 30% (TrEMBL:Q8Y2M5). No domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 783562 4608496 azo0732 Azoarcus sp. BH72 hypothetical protein YP_932236.1 782852 R 62928 CDS YP_932237.1 119897024 4606539 complement(783572..784363) 1 NC_008702.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase 784363 baf 4606539 baf Azoarcus sp. BH72 pantothenate kinase YP_932237.1 783572 R 62928 CDS YP_932238.1 119897025 4608375 complement(784360..785088) 1 NC_008702.1 BirA bifunctional protein [Includes: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase (EC 6.3.4.15) (Biotin--protein ligase)]. birA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-coa:carbon- dioxide ligase. this protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins. InterPro: Biotin--acetyl-CoA-carboxylase ligase birA_ligase: biotin--acetyl-CoA-carboxyla; Specificity unclear; biotin--[acetyl-CoA-carboxylase] ligase 785088 birA 4608375 birA Azoarcus sp. BH72 biotin--[acetyl-CoA-carboxylase] ligase YP_932238.1 784360 R 62928 CDS YP_932239.1 119897026 4608396 complement(785250..786680) 1 NC_008702.1 Nitrogen regulation protein NR(I),; High confidence in function and specificity; nitrogen regulation protein NR(I) 786680 ntrC 4608396 ntrC Azoarcus sp. BH72 nitrogen regulation protein NR(I) YP_932239.1 785250 R 62928 CDS YP_932240.1 119897027 4609414 complement(786724..787797) 1 NC_008702.1 Nitrogen regulation protein NR(II),; High confidence in function and specificity; nitrogen regulation protein NR(II) 787797 ntrB 4609414 ntrB Azoarcus sp. BH72 nitrogen regulation protein NR(II) YP_932240.1 786724 R 62928 CDS YP_932241.1 119897028 4609413 complement(787859..788335) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to cv3590 of C. violaceum of 39% (trembl|Q7NS37(SRS)) no domains predicted signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 788335 4609413 azo0737 Azoarcus sp. BH72 hypothetical protein YP_932241.1 787859 R 62928 CDS YP_932242.1 119897029 4606540 complement(788464..789873) 1 NC_008702.1 Glutamine synthetase I (GS) plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. Similar to pir|G83005 (70%) and to pir|AJECQ (66%). Pfam (PF00120): Glutamine synthetase, catalytic domain Pfam (PF03951): Glutamine synthetase, beta-Grasp domain; High confidence in function and specificity; glutamine synthetase 789873 glnA 4606540 glnA Azoarcus sp. BH72 glutamine synthetase YP_932242.1 788464 R 62928 CDS YP_932243.1 119897030 4608894 complement(790055..791269) 1 NC_008702.1 CzcD: Members of this family (TC 2.A.4) are integral membrane proteins that are found to increase tolerance to divalent metal ions such as cadmium, zinc,and cobalt. These proteins are thought to be efflux pumps that remove these ions from cells. (Slc30a subfamily). Similar to the cobalt-zinc-cadmium resistance protein CzcD (cation efflux system protein) from Alcaligenes eutrophus (Ralstonia eutropha). SWISSPROT:CZCD_ALCEU.P13512. InterPro:IPR002524; Cation_efflux.InterPro: Cation efflux family. Pfam:PF01545; Cation_efflux; 1. TIGRFAMs:TIGR01297; CDF; 1. TMHelix: 4.; High confidence in function and specificity; putative cobalt-zinc-cadmium resistance protein 791269 czcD1 4608894 czcD1 Azoarcus sp. BH72 putative cobalt-zinc-cadmium resistance protein YP_932243.1 790055 R 62928 CDS YP_932244.1 119897031 4608573 791287..791739 1 NC_008702.1 Similar to Rhodanese domain protein, a sulphurtransferase involved in cyanide detoxification. InterPro: Rhodanese/cdc25 fold; Specificity unclear; putative sulfurtransferase 791739 4608573 azo0740 Azoarcus sp. BH72 putative sulfurtransferase YP_932244.1 791287 D 62928 CDS YP_932245.1 119897032 4606541 791814..792914 1 NC_008702.1 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate; quinolinate synthetase 792914 nadA 4606541 nadA Azoarcus sp. BH72 quinolinate synthetase YP_932245.1 791814 D 62928 CDS YP_932246.1 119897033 4609302 792936..793685 1 NC_008702.1 Conserved hypothetical protein. Homology to rs04427 of R. solanacearum of 55% (trembl|Q8Y267). Pfam: Endonuclease/Exonuclease/phosphatase family (PF03372). Interpro: Endonuclease/Exonuclease/phosphatase family (IPR005135). no signal peptide. no TMHs; hypothetical protein 793685 4609302 azo0742 Azoarcus sp. BH72 hypothetical protein YP_932246.1 792936 D 62928 CDS YP_932247.1 119897034 4606542 793682..794839 1 NC_008702.1 Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Affects resistance to the gyrase inhibitor novobiocin. Entry name: CLS_ECOLI Primary accession number P31071 InterPro IPR001736; PLD. Pfam PF00614; PLDc; 2 Identity:- 28% Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; putative cardiolipin synthetase 794839 cls1 4606542 cls1 Azoarcus sp. BH72 putative cardiolipin synthetase YP_932247.1 793682 D 62928 CDS YP_932248.1 119897035 4608492 complement(794846..795355) 1 NC_008702.1 Protein yccU. TREMBL:Q982W3: 50% identity, 70% similarity. This domain has a Rossmann fold and is found in a number of proteins including succinyl CoA synthetases, malate and ATP-citrate ligases. InterPro:IPR003781; CoA_binding. Pfam: PF02629; CoA_binding FRU: PTS enzyme-IIB fructose permease No signal peptide. No transmembrane helices present.; Function unclear; hypothetical protein 795355 4608492 azo0744 Azoarcus sp. BH72 hypothetical protein YP_932248.1 794846 R 62928 CDS YP_932249.1 119897036 4606543 complement(795366..796367) 1 NC_008702.1 Putative glutamyl-tRNA synthetase-related protein,58% identity to TrEMBl; Q5P2J9,47% identity to TrEMBL; Q7NSJ1. Has PF00749, tRNA synthetases class I (E and Q),catalytic domain.Other tRNA synthetase sub-families are too dis; Specificity unclear; glutamate-tRNA ligase 796367 4606543 azo0745 Azoarcus sp. BH72 glutamate-tRNA ligase YP_932249.1 795366 R 62928 CDS YP_932250.1 119897037 4606544 796481..798106 1 NC_008702.1 Hypothetical membrane protein. Homology to la0834 of L. interrogans of 25% (trembl|Q8F7U9). InterPro: Dolichyl-phosphate-mannose-protein mannosyltransferase (IPR003342). Dolichyl-phosphate-mannose-protein mannosyltransferases belong to the glycosyltransferase family 39 and are responsible for O-linked glycosylation of proteins. They catalyse the reaction: Dolichyl phosphate D-mannose + protein -> dolichyl phosphate + O-D-mannosyl-protein. Pfam: Dolichyl-phosphate-mannose-protein mannosyltransferase. signal peptide. 10 TMHs; hypothetical protein 798106 4606544 azo0746 Azoarcus sp. BH72 hypothetical protein YP_932250.1 796481 D 62928 CDS YP_932251.1 119897038 4606545 798162..799442 1 NC_008702.1 40% GH_BNR. Pfam:PF02012; BNR; 2. TMHelix:1. Signal peptide: present.; Function unclear; hypothetical protein 799442 4606545 azo0747 Azoarcus sp. BH72 hypothetical protein YP_932251.1 798162 D 62928 CDS YP_932252.1 119897039 4606546 complement(799936..800856) 1 NC_008702.1 Probable cellobiose phosphorylase, 31% identity to TrEMBL;Q7NYW4. Has PF04794, YdjC-like protein;IPR006879; Family of YdjC-like proteins. This region is possibly involved in the the cleavage of cellobiose-phosphate.; Function unclear; cellobiose phosphorylase 800856 4606546 azo0748 Azoarcus sp. BH72 cellobiose phosphorylase YP_932252.1 799936 R 62928 CDS YP_932253.1 119897040 4606547 800954..801982 1 NC_008702.1 Conserved hypothetical glycosyl transferase. Homology to cc2889 of. C. crescentus (trembl|Q9A4E5). Pfam: Glycosyl transferase family 2(PF00535). Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl-galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids. Interpro: Glycosyl transferase family 2 (IPR001173). no signal peptide. 2 TMHs.; Conserved hypothetical protein; glycosyl transferase family protein 801982 4606547 azo0749 Azoarcus sp. BH72 glycosyl transferase family protein YP_932253.1 800954 D 62928 CDS YP_932254.1 119897041 4606548 802057..803655 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q89QQ8: 34% identity This is a subfamily of bacterial binding-protein-dependent transport systems family, and includes transport system permeases involved in the transport across the membrane of several compounds. This entry contains the inner components of this multicomponent transport system InterPro: IPR000522; FecD. Pfam: PF01032; FecCD ncs1: NCS1 nucleoside transporter fam Nonsecretory protein with signal peptide probability 0.203 (Signal P predicted). TMHMM predicted 9 transmembrane helices.; Function unclear; hypothetical protein 803655 4606548 azo0750 Azoarcus sp. BH72 hypothetical protein YP_932254.1 802057 D 62928 CDS YP_932255.1 119897042 4606549 803652..804053 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA4820 of P. aeruginosa of 38% (trembl|Q9HUZ0(SRS)) Has PF04138, GtrA-like protein;IPR007267;Members of this family are predicted to be integral membrane proteins with three or four transmembrane spans. They are involved in the synthesis of cell surface polysaccharides. The GtrA family are a subset of this family. GtrA is predicted to be an integral membrane protein with 4 transmembrane spans. It is involved is in O antigen modification by Shigella flexneri bacteriophage X (SfX), but does not determine the specificity of glucosylation. Its function remains unknown, but it may play a role in translocation of undecaprenyl phosphate linked glucose (UndP-Glc) across the cytoplasmic membrane. no signal peptide 4 TMHs; Conserved hypothetical protein; hypothetical protein 804053 4606549 azo0751 Azoarcus sp. BH72 hypothetical protein YP_932255.1 803652 D 62928 CDS YP_932256.1 119897043 4606550 complement(804105..805196) 1 NC_008702.1 translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1; GTP-dependent nucleic acid-binding protein EngD 805196 ychF 4606550 ychF Azoarcus sp. BH72 GTP-dependent nucleic acid-binding protein EngD YP_932256.1 804105 R 62928 CDS YP_932257.1 119897044 4610159 complement(805193..805849) 1 NC_008702.1 Enables the recycling of peptidyl-tRNAs produced at termination of translation; peptidyl-tRNA hydrolase 805849 pth 4610159 pth Azoarcus sp. BH72 peptidyl-tRNA hydrolase YP_932257.1 805193 R 62928 CDS YP_932258.1 119897045 4609646 complement(805889..806491) 1 NC_008702.1 the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response; 50S ribosomal protein L25/general stress protein Ctc 806491 rplY 4609646 rplY Azoarcus sp. BH72 50S ribosomal protein L25/general stress protein Ctc YP_932258.1 805889 R 62928 CDS YP_932259.1 119897046 4609784 complement(806573..807523) 1 NC_008702.1 Ribose-phosphate pyrophosphokinase (RPPK) (Phosphoribosyl pyrophosphate synthetase) (P-Rib-PP synthetase) (PRPP synthetase); High confidence in function and specificity; ribose-phosphate diphosphokinase 807523 prsA 4609784 prsA Azoarcus sp. BH72 ribose-phosphate diphosphokinase YP_932259.1 806573 R 62928 CDS YP_932260.1 119897047 4609990 complement(807698..808546) 1 NC_008702.1 An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 808546 ipk 4609990 ipk Azoarcus sp. BH72 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase YP_932260.1 807698 R 62928 CDS YP_932261.1 119897048 4609096 complement(808543..809124) 1 NC_008702.1 conserved hypothetical outer-membrane lipoprotein. Homology to lolB of C. violaceum of 32% (sprot|LOLB_CHRVO) Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein (By similarity). Tigrfam: lolB: outer membrane lipoprotein LolB Pfam: outer membrane lipoprotein LolB signal peptide no TMHs; Family membership; outer membrane lipoprotein 809124 lolB 4609096 lolB Azoarcus sp. BH72 outer membrane lipoprotein YP_932261.1 808543 R 62928 CDS YP_932262.1 119897049 4609169 complement(809121..810836) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to cv4061 of C. violaceum of 37% (trembl|Q7NQS6). InterPro: TPR repeat (IPR001440) signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 810836 4609169 azo0758 Azoarcus sp. BH72 hypothetical protein YP_932262.1 809121 R 62928 CDS YP_932263.1 119897050 4606551 810989..811807 1 NC_008702.1 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases; formamidopyrimidine-DNA glycosylase 811807 mutM 4606551 mutM Azoarcus sp. BH72 formamidopyrimidine-DNA glycosylase YP_932263.1 810989 D 62928 CDS YP_932264.1 119897051 4609297 811931..813898 1 NC_008702.1 TREMBL:Q8Y2D6:42% identity, 59% similarity Hypothetical protein in xynA 3region (ORF6) (Fragment). Pfam:dynamin_2 :Dynamin central region; FH2:Formin Homology 2 Domain surE: stationary-phase survival prote No transmembrane helices(TMHMM predicted); Function unclear; hypothetical protein 813898 4609297 azo0760 Azoarcus sp. BH72 hypothetical protein YP_932264.1 811931 D 62928 CDS YP_932265.1 119897052 4606552 complement(813966..814223) 1 NC_008702.1 Probable ferredoxin. Homology to fdx of C. vinosum of 67% (sprot|FER_CHRVI). Ferredons are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Pfam: 4Fe-4S binding domain no signal peptide no TMHs; Family membership; ferredoxin 814223 fdx1 4606552 fdx1 Azoarcus sp. BH72 ferredoxin YP_932265.1 813966 R 62928 CDS YP_932266.1 119897053 4608745 complement(814240..814731) 1 NC_008702.1 Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA; phosphopantetheine adenylyltransferase 814731 coaD 4608745 coaD Azoarcus sp. BH72 phosphopantetheine adenylyltransferase YP_932266.1 814240 R 62928 CDS YP_932267.1 119897054 4608495 complement(814728..815249) 1 NC_008702.1 Putative methylase; Family membership; putative methylase 815249 4608495 azo0763 Azoarcus sp. BH72 putative methylase YP_932267.1 814728 R 62928 CDS YP_932268.1 119897055 4606553 complement(815264..816616) 1 NC_008702.1 Hypothetical zinc protease-like protein y4wB. TREMBL:Q8Y2E8: 43% identity; 57% similarity. InterPro: Insulinase family (Peptidase family M16) InterPro:IPR001431; Peptidase_M16. IPR007863: Peptidase_M16_C. Pfam:PF00675; Peptidase_M16 (Insulinase); homoserine_dh; aldehyde dehydrogenase; PF05193:Peptidase_M16_C; precor6x_red: precorrin-6x reductase TIGRFAM:folate/biopterin transporter SignalP predicted signal peptide and TMHMM predicted transmembrane helix; Specificity unclear; Zn dependent peptidase 816616 4606553 azo0764 Azoarcus sp. BH72 Zn dependent peptidase YP_932268.1 815264 R 62928 CDS YP_932269.1 119897056 4606554 complement(816645..818111) 1 NC_008702.1 TREMBL:Q8Y2E9: 55% identity, 68% similarity. Hypothetical zinc protease y4wA. InterPro:IPR001431; Peptidase_M16. IPR007863: Peptidase_M16_C. cofactor:binds 1 zinc ion per subunit (by similarity). belongs to peptidase family m16. similarity:to y4wb. Pfam:PF00675; Peptidase_M16; 1. PF05193:Peptidase_M16_C TIGRFAM: uxuA SignalP predicted signal peptide and TMHMM predicted transmembrane helices menD: 2-succinyl-6-hydroxy-24-cyclohex; Specificity unclear; Zn dependent peptidase 818111 4606554 azo0765 Azoarcus sp. BH72 Zn dependent peptidase YP_932269.1 816645 R 62928 CDS YP_932270.1 119897057 4606555 818232..819365 1 NC_008702.1 Probable signal recognition particle-docing protein FtsY Homology to ftsY of C. violaceum of 65% (trembl|Q7NQD7) In E.coli, ftsY is an essential gene located in an operon with cell division genes ftsE and ftsX, but its apparent function is as the signal recognition particle docking protein. Pfam: SRP54-type protein, helical bundle domain (PF02881); SRP54-type protein, GTPase domain Tigrfam: ftsY: signal recognition particale-docking protein FtsY no signal peptide no TMHs; High confidence in function and specificity; signal recognition particle-docing protein FtsY 819365 FtsY 4606555 FtsY Azoarcus sp. BH72 signal recognition particle-docing protein FtsY YP_932270.1 818232 D 62928 CDS YP_932271.1 119897058 4608842 819362..820021 1 NC_008702.1 High confidence in function and specificity; cell division ATP-binding protein FtsE 820021 FtsE 4608842 FtsE Azoarcus sp. BH72 cell division ATP-binding protein FtsE YP_932271.1 819362 D 62928 CDS YP_932272.1 119897059 4608833 820018..820914 1 NC_008702.1 Putative cell division protein FtsX. Homology to ftsX of E. coli of 33% (sprot|FTSX_ECOLI). FtsX is an integral membrane protein encoded in the same operon as signal recognition particle docking protein FtsY and FtsE. Might be a permease. Tigrfam: ftsX: cell division ABC transporter protein probable signal peptide probable 4 TMHs; Family membership; cell division protein FtsX 820914 FtsX 4608833 FtsX Azoarcus sp. BH72 cell division protein FtsX YP_932272.1 820018 D 62928 CDS YP_932273.1 119897060 4608841 821109..821672 1 NC_008702.1 Alkyl hydroperoxide reductase subunit C. Homology to aphC of X. campestris of 84% (trembl|O06464). Directly reduces organic hydroperoxides in its reduced dithiol form. InterPro: Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (IPR000866) Pfam: AhpC/TSA family no signal peptide no TMHs; High confidence in function and specificity; alkyl hydroperoxide reductase subunit C 821672 ahpC 4608841 ahpC Azoarcus sp. BH72 alkyl hydroperoxide reductase subunit C YP_932273.1 821109 D 62928 CDS YP_932274.1 119897061 4608287 821802..823355 1 NC_008702.1 Alkyl hydroperoxide reductase subunit F. Homology to aphF of X. campestris of 71% (sprot|AHPF_XANCH) Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AHPC protein. Pfam: Pyridine nucleotide-disulphide oxidareductase no TMHs; High confidence in function and specificity; alkyl hydroperoxide reductase subunit F 823355 ahpF 4608287 ahpF Azoarcus sp. BH72 alkyl hydroperoxide reductase subunit F YP_932274.1 821802 D 62928 CDS YP_932275.1 119897062 4608288 complement(824754..826058) 1 NC_008702.1 Probable C-5 cytosine-specific DNA methylase InterPro: C-5 cytosine-specific DNA methylase; C-5 cytosine-specific DNA methylase 826058 4608288 azo0771 Azoarcus sp. BH72 C-5 cytosine-specific DNA methylase YP_932275.1 824754 R 62928 CDS YP_932276.1 119897063 4606556 complement(826594..827043) 1 NC_008702.1 Ribonuclease H (EC 3.1.26.4) (RNase H). This enzyme is an endonuclease that degrades the RNA of RNA-DNA hybrids specifically (By similarity). InterPro: RNase H; High confidence in function and specificity; RnhA1 protein 827043 rnhA1 4606556 rnhA1 Azoarcus sp. BH72 RnhA1 protein YP_932276.1 826594 R 62928 CDS YP_932277.1 119897064 4609752 complement(827040..827819) 1 NC_008702.1 Hypothetical protein , 32% identity to TrEMBL;Q8NZP4 No domains, repeats, motifs or features present.; hypothetical protein 827819 4609752 azo0773 Azoarcus sp. BH72 hypothetical protein YP_932277.1 827040 R 62928 CDS YP_932278.1 119897065 4606557 complement(828127..828822) 1 NC_008702.1 hypothetical protein 828822 4606557 azo0774 Azoarcus sp. BH72 hypothetical protein YP_932278.1 828127 R 62928 CDS YP_932279.1 119897066 4606558 complement(829733..830302) 1 NC_008702.1 Putative DNA-invertase from lambdoid prophage Rac. InterPro: Site-specific recombinase Pfam: Resolvase; Family membership; putative resolvase 830302 4606558 azo0775 Azoarcus sp. BH72 putative resolvase YP_932279.1 829733 R 62928 CDS YP_932280.1 119897067 4606559 complement(830685..832355) 1 NC_008702.1 Family membership; putative integrase 832355 4606559 azo0776 Azoarcus sp. BH72 putative integrase YP_932280.1 830685 R 62928 CDS YP_932281.1 119897068 4610010 832729..833076 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 833076 4610010 azo0777 Azoarcus sp. BH72 hypothetical protein YP_932281.1 832729 D 62928 CDS YP_932282.1 119897069 4606561 833091..834440 1 NC_008702.1 catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A); (dimethylallyl)adenosine tRNA methylthiotransferase 834440 4606561 azo0778 Azoarcus sp. BH72 (dimethylallyl)adenosine tRNA methylthiotransferase YP_932282.1 833091 D 62928 CDS YP_932283.1 119897070 4606562 834440..835423 1 NC_008702.1 PhoH-like protein,; High confidence in function and specificity; PhoH-like protein 835423 ybeZ 4606562 ybeZ Azoarcus sp. BH72 PhoH-like protein YP_932283.1 834440 D 62928 CDS YP_932284.2 228906475 4610147 835359..836174 1 NC_008702.1 contains unknown N-terminal domain/putative metalloprotease C-terminal domain; unkown domain/metalloprotease fusion protein 836174 4610147 azo0780 Azoarcus sp. BH72 unkown domain/metalloprotease fusion protein YP_932284.2 835359 D 62928 CDS YP_932285.1 119897072 4606563 836270..837115 1 NC_008702.1 Magnesium and cobalt efflux protein corC. Plays a role in the transport of magnesium and cobalt ions. Also mediates transport of cobalt and nickel. 43% CBS_domain.IPR005170; CorC_transpt-asc. Pfam:PF00571; CBS; 2.PF03471; CorC_HlyC; 1. Belongs to the UPF0053 family.Contains 2 cbs domains.; High confidence in function and specificity; putative magnesium and cobalt efflux protein 837115 corC 4606563 corC Azoarcus sp. BH72 putative magnesium and cobalt efflux protein YP_932285.1 836270 D 62928 CDS YP_932286.1 119897073 4608519 837115..838629 1 NC_008702.1 Putative apolipoprotein N-acyltransferase. Homology to cutE of E. coli of 36% (sprot|LNT_ECOLI). Transfers the fatty acyl group on membrane lipoproteins. InterPro: Apolipoprotein N-acyltransferase (IPR004563); Carbon-nitrogen hydrolase (IPR003010) Tigrfam: lnt: apolipoprotein N-acyltransferase Pfam: Carbon-nitrogen hydrolase signal peptide 6 TMHs; High confidence in function and specificity; putative apolipoprotein N-acyltransferase 838629 lnt 4608519 lnt Azoarcus sp. BH72 putative apolipoprotein N-acyltransferase YP_932286.1 837115 D 62928 CDS YP_932287.1 119897074 4609167 838754..839893 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family, TREMBL:Q7NHP0 (27% identity); SignalP predicting signal peptide. TC (8.A.1): The Membrane Fusion Protein (MFP) Family.; Family membership; membrane fusion protein 839893 4609167 azo0783 Azoarcus sp. BH72 membrane fusion protein YP_932287.1 838754 D 62928 CDS YP_932288.1 119897075 4606564 839904..842957 1 NC_008702.1 AcrB/AcrD/AcrF family protein. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q9A3K6 (38% identity); TREMBL:Q89XF8 (40% identity). InterPro (IPR001036): Acriflavin resistance protein. Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TMHMM predicting 12 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 842957 4606564 azo0784 Azoarcus sp. BH72 RND efflux transporter permease YP_932288.1 839904 D 62928 CDS YP_932289.1 119897076 4606565 843084..844010 1 NC_008702.1 glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase subunit alpha 844010 glyQ 4606565 glyQ Azoarcus sp. BH72 glycyl-tRNA synthetase subunit alpha YP_932289.1 843084 D 62928 CDS YP_932290.1 119897077 4606566 844007..846094 1 NC_008702.1 Glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Glycine--tRNA ligase beta chain) (GlyRS).; High confidence in function and specificity; glycyl-tRNA synthetase subunit beta 846094 glyS 4606566 glyS Azoarcus sp. BH72 glycyl-tRNA synthetase subunit beta YP_932290.1 844007 D 62928 CDS YP_932291.1 119897078 4608925 846144..846677 1 NC_008702.1 Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate; D,D-heptose 1,7-bisphosphate phosphatase 846677 4608925 azo0787 Azoarcus sp. BH72 D,D-heptose 1,7-bisphosphate phosphatase YP_932291.1 846144 D 62928 CDS YP_932292.1 119897079 4606567 846695..847453 1 NC_008702.1 Acyl-[acyl-carrier protein] can also acts as acyl donor.The animal enzyme is specific for the transfer of unsaturated fatty acyl groups. Catalytic Activity:- Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Entry name TREMBL:Q82VB2 Prim. accession # Q82VB2 InterPro:- IPR002123; Acyltransferase. Pfam:- PF01553; Acyltransferase; 1. Identity:- 56% Prediction: Signal peptide Signal peptide probability: 0.907 Number of predicted TMHs: 1; Family membership; acyltransferase 847453 4606567 azo0788 Azoarcus sp. BH72 acyltransferase YP_932292.1 846695 D 62928 CDS YP_932293.1 119897080 4606568 complement(847542..848102) 1 NC_008702.1 Probable phasin. Homology to p8 of Sphingomonas sp. A1 of 69% (gi|51773776|dbj|BAD38885.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; Family membership; phasin 848102 4606568 azo0789 Azoarcus sp. BH72 phasin YP_932293.1 847542 R 62928 CDS YP_932294.1 119897081 4606569 complement(848399..849175) 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 849175 paaF1 4606569 paaF1 Azoarcus sp. BH72 enoyl-CoA hydratase YP_932294.1 848399 R 62928 CDS YP_932295.1 119897082 4609470 complement(849202..849774) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc0410 of R. solanacearum of 42% (trembl|Q8Y2C6(SRS)) Pfam: OstA-like protein This family of proteins are mostly uncharacterised. However the family does include E. coli OstA P31554 that has been characterised as an organic solvent tolerance protein. signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 849774 4609470 azo0791 Azoarcus sp. BH72 hypothetical protein YP_932295.1 849202 R 62928 CDS YP_932296.1 119897083 4606570 complement(849771..850349) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA1318 of Azoarcus sp. EbN1 of 47% (gnl|keqq|eba:ebA1318(KEGG)). Has PF06835, Protein of unknown function (DUF1239);IPR010664;This family consists of several hypothetical bacterial proteins of around 190 residues in length. The function of this family is unknown. No TMHs. Signal peptide.; Conserved hypothetical protein; hypothetical protein 850349 4606570 azo0792 Azoarcus sp. BH72 hypothetical protein YP_932296.1 849771 R 62928 CDS YP_932297.1 119897084 4606571 complement(850359..850880) 1 NC_008702.1 Catalyzes the hydrolysis of KDO 8-P to KDO and inorganic phosphate TREMBL:Q9JSU3: 43% identity, 59% similarity InterPro: IPR006549; HAD-SF-IIIA. IPR005834; Hydrolase. IPR008230; Sugar_Ptase. IPR010023; YrbI_phosphatas. Pfam: PF00702; Hydrolase TIGR00099: conserved hypothetical prote Absence of transmembrane helices.; Specificity unclear; phosphatase 850880 4606571 azo0793 Azoarcus sp. BH72 phosphatase YP_932297.1 850359 R 62928 CDS YP_932298.1 119897085 4606572 complement(850880..851875) 1 NC_008702.1 Arabinose 5-phosphate isomerase (EC 5.3.1.13). Catalyzes the interconversion of D-arabinose 5-phosphate and D-ribulose 5-phosphate (By similarity). InterPro: KpsF/GutQ family protein kpsF: KpsF/GutQ family protein; High confidence in function and specificity; sugar-phosphate isomerase 851875 4606572 azo0794 Azoarcus sp. BH72 sugar-phosphate isomerase YP_932298.1 850880 R 62928 CDS YP_932299.1 119897086 4606573 852075..854042 1 NC_008702.1 Probable glutathione-regulated potassium-efflux system protein (K(+)/H(+) antiporter). Transport system that facilitate potassium-efflux possibly by potassium-proton antiport. 50% Na_H_porter.IPR006037; TrkAC.IPR003148; TrkA_N. Pfam: PF00999; Na_H_Exchanger; 1.PF02080; TrkA_C; 1.PF02254; TrkA_N; 1. TMhelix: 11.; High confidence in function and specificity; putative glutathione-regulated potassium-efflux system protein 854042 4606573 azo0795 Azoarcus sp. BH72 putative glutathione-regulated potassium-efflux system protein YP_932299.1 852075 D 62928 CDS YP_932300.1 119897087 4606574 complement(854050..854697) 1 NC_008702.1 Conserved hypothetical membrabe protein. Homology to Mflag03002444 of Methylobacillus flagellatus of 48% (gi|46120631|ref|ZP_00201765.1|(NBCI ENTREZ)). no domains predicted. No signal peptide. 1 TMHs; hypothetical protein 854697 4606574 azo0796 Azoarcus sp. BH72 hypothetical protein YP_932300.1 854050 R 62928 CDS YP_932301.1 119897088 4606575 complement(854732..855430) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002886 of Dechloromonas aromatica of 31% (gi|53729825|ref|ZP_00150246.2|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide.; hypothetical protein 855430 4606575 azo0797 Azoarcus sp. BH72 hypothetical protein YP_932301.1 854732 R 62928 CDS YP_932302.1 119897089 4606576 complement(855418..856656) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 856656 4606576 azo0798 Azoarcus sp. BH72 hypothetical protein YP_932302.1 855418 R 62928 CDS YP_932303.1 119897090 4606577 complement(856671..857057) 1 NC_008702.1 Hypothetical secreted protein. no homology with the data bank. no domains predicted. signal peptide. no TMHS; hypothetical protein 857057 4606577 azo0799 Azoarcus sp. BH72 hypothetical protein YP_932303.1 856671 R 62928 CDS YP_932304.1 119897091 4606578 complement(857054..857644) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 857644 4606578 azo0800 Azoarcus sp. BH72 hypothetical protein YP_932304.1 857054 R 62928 CDS YP_932305.1 119897092 4606579 complement(857641..859065) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. Signal peptide present. 1 TMH present.; hypothetical protein 859065 4606579 azo0801 Azoarcus sp. BH72 hypothetical protein YP_932305.1 857641 R 62928 CDS YP_932306.1 119897093 4606580 complement(859072..860277) 1 NC_008702.1 Type II secretory pathway component F,; Specificity unclear; general secretion pathway protein F 860277 gspF1 4606580 gspF1 Azoarcus sp. BH72 general secretion pathway protein F YP_932306.1 859072 R 62928 CDS YP_932307.1 119897094 4608952 complement(860274..861974) 1 NC_008702.1 Bacterial type II secretion system protein E,; Specificity unclear; general secretion pathway protein E 861974 gspE1 4608952 gspE1 Azoarcus sp. BH72 general secretion pathway protein E YP_932307.1 860274 R 62928 CDS YP_932308.1 119897095 4608949 complement(861980..862615) 1 NC_008702.1 Hypothetical protein. No homology to a protein of similar size. No domains predicted. No signal peptide. No TMHs; hypothetical protein 862615 4608949 azo0804 Azoarcus sp. BH72 hypothetical protein YP_932308.1 861980 R 62928 CDS YP_932309.1 119897096 4606581 complement(862681..864423) 1 NC_008702.1 Type II secretory pathway component D, weak similarity to SWISSPROT:HOFQ_ECOLI (18%). A number of proteins are involved in the general secretion pathway (GSP); one of these is known as protein D (GSPD protein). Protein D is involved in the type II general secretion pathway within Gram-negative bacteria, a signal sequence-dependent process responsible for protein export. InterPro (PF00263): General (type II) secretion pathway (GSP) D protein; Specificity unclear; general secretion pathway protein D 864423 gspD1 4606581 gspD1 Azoarcus sp. BH72 general secretion pathway protein D YP_932309.1 862681 R 62928 CDS YP_932310.1 119897097 4608947 864497..865330 1 NC_008702.1 Conserved hypothetical ABC transporter ATP-binding protein. Homology to rs01335 of R. solanacearum of 67% (trembl|Q8XV69). ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. They are minimally composed of four domains, with two transmembrane domains (TMDs) responsible for allocrite binding and transport and two nucleotide-binding domains (NBDs) responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs. Both NBDs are capable of ATP hydrolysis. Pfam: ABC transporter (PF00005). No signal peptide. No TMHs; Family membership; ABC transporter ATP-binding protein 865330 4608947 azo0806 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932310.1 864497 D 62928 CDS YP_932311.1 119897098 4606582 865327..866118 1 NC_008702.1 Conserved hypothetical membrane protein Similar to TREMBL:Q7P0W7 (65% identity); TREMBL:Q82SF6 (65% identity); TREMBL:Q7VSZ9 (57% identity). InterPro (IPR003453): Domain of unknown function DUF140. Pfam (PF02405): Domain of unknown function DUF140. TIGRFAM (TIGR00056): Conserved hypothetical protein. TMHMM reporting five transmembrane helices.; hypothetical protein 866118 4606582 azo0807 Azoarcus sp. BH72 hypothetical protein YP_932311.1 865327 D 62928 CDS YP_932312.1 119897099 4606583 866118..866591 1 NC_008702.1 Conserved hypothetical secreted protein. Similar to TREMBL:Q8XV71 (59% identity); TREMBL:Q82SF7 (55% identity); TREMBL:Q7VSZ8 (55% identity). Pfam (PF02470): mce related protein. This family of proteins contains the mce (mycobacterial cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within,the macrophage. This family contains proteins of unknown function from other bacteria. SignalP reoprting signal peptide. TMH in signal peptide; Family membership; hypothetical protein 866591 4606583 azo0808 Azoarcus sp. BH72 hypothetical protein YP_932312.1 866118 D 62928 CDS YP_932313.1 119897100 4606584 866632..867492 1 NC_008702.1 Putative surface lipoprotein. Homology to vacJ of S. flexneri of 26% (sprot|VACJ_SHIFL) Required for intercellular spreading. Pfam: VacJ like lipoprotein signal peptide no TMHs; Family membership; putative surface lipoprotein 867492 vacJ 4606584 vacJ Azoarcus sp. BH72 putative surface lipoprotein YP_932313.1 866632 D 62928 CDS YP_932314.1 119897101 4610088 867498..868145 1 NC_008702.1 Conserved hypothetical secreted protein. Similar to TREMBL:Q82SF8 (54% identity); TREMBL:Q8XV73 (42% identity); TREMBL:Q7VSZ6 (40% identity). SignalP reporting signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 868145 4610088 azo0810 Azoarcus sp. BH72 hypothetical protein YP_932314.1 867498 D 62928 CDS YP_932315.1 119897102 4606585 868216..868497 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc2957 of R. solanacearum of 42% (trembl|Q8XV74). Pfam: STAS domain The STAS (after Sulphate Transporter and AntiSigma factor antagonist) domain is found in the C terminal region of Sulphate transporters and bacterial antisigma factor antagonists. It has been suggested that this domain may have a general NTP binding function. no signal peptide no TMHs; hypothetical protein 868497 4606585 azo0811 Azoarcus sp. BH72 hypothetical protein YP_932315.1 868216 D 62928 CDS YP_932316.1 119897103 4606586 868505..869401 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q7P0X8 (62% identity); TREMBL:Q82SG0 (58% identity). InterPro (IPR003593): AAA ATPase. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003439): ABC transporter. Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; ABC transporter ATP-binding protein 869401 4606586 azo0812 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932316.1 868505 D 62928 CDS YP_932317.1 119897104 4606587 869394..870194 1 NC_008702.1 Similar to TREMBL:Q8XV76 (69% identity); TREMBL:Q7P0X7 (66% identity); TREMBL:Q82SG1 (64% identity). InterPro (IPR000412): ABC transporter family 2. Pfam (PF01061): ABC-2 type transporter. TMHMM reporting seven transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Specificity unclear; ABC-2 type transporter permease 870194 4606587 azo0813 Azoarcus sp. BH72 ABC-2 type transporter permease YP_932317.1 869394 D 62928 CDS YP_932318.1 119897105 4606588 870223..870480 1 NC_008702.1 Conserved hypothetical BolA-like protein. Homology to ne2377 of N. europaea of 47% (trembl|Q82SG2). InterPro: BolA-like protein This family consist of the morphoprotein BolA from E. coli and its various homologues. In E. coli over expression of this protein causes round morphology and may be involved in switching the cell between elongation and septation systems during cell division. The expression of BolA is growth rate regulated and is induced during the transition into the the stationary phase. BolA is also induced by stress during early stages of growth and may have a general role in stress response. It has also been suggested that BolA can induce the transcription of penicillin binding proteins 6 and 5. Pfam: BolA-like binding protein no signal peptide no TMHs; Function unclear; BolA-like protein 870480 4606588 azo0814 Azoarcus sp. BH72 BolA-like protein YP_932318.1 870223 D 62928 CDS YP_932319.1 119897106 4606589 870517..871767 1 NC_008702.1 adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active; UDP-N-acetylglucosamine 1-carboxyvinyltransferase 871767 murA 4606589 murA Azoarcus sp. BH72 UDP-N-acetylglucosamine 1-carboxyvinyltransferase YP_932319.1 870517 D 62928 CDS YP_932320.1 119897107 4609287 complement(871799..872206) 1 NC_008702.1 Regulator of nucleoside diphosphate kinase. RNK AND SSPA CAN FUNCTIONALLY REPLACE P.AERUGINOSA ALGINATE REGULATORY GENE ALGR2. 49% 1.; High confidence in function and specificity; putative regulator of nucleoside diphosphate kinase 872206 rnk 4609287 rnk Azoarcus sp. BH72 putative regulator of nucleoside diphosphate kinase YP_932320.1 871799 R 62928 CDS YP_932321.1 119897108 4609755 872316..872990 1 NC_008702.1 short form of enzyme; requires HisZ for function; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms heteromultimer of HisG and HisZ; ATP phosphoribosyltransferase catalytic subunit 872990 hisG 4609755 hisG Azoarcus sp. BH72 ATP phosphoribosyltransferase catalytic subunit YP_932321.1 872316 D 62928 CDS YP_932322.1 119897109 4609006 872987..874297 1 NC_008702.1 catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer; histidinol dehydrogenase 874297 hisD 4609006 hisD Azoarcus sp. BH72 histidinol dehydrogenase YP_932322.1 872987 D 62928 CDS YP_932323.1 119897110 4609003 complement(874352..875542) 1 NC_008702.1 Aminotransferases share certain mechanistic features with other pyridoxal-phosphate dependent enzymes,such as the covalent binding of the pyridoxal-phosphate group to a lysine residue. On the basis of sequence similarity, these various enzymes can be grouped into subfamilies; these sequences are defined by the aminotransferases class-I pyridoxal-phosphate attachment site signature, which contains the lysine residue involved in pyridoxal-phosphate binding. Similar to pir|D83057 (55%) and to trembl|Q8P544 (55%). Pfam (PF00155): Aminotransferases class-I pyridoxal-phosphate-binding site HTH reporting nucleic acid binding motif.; Specificity unclear; hypothetical protein 875542 aspC 4609003 aspC Azoarcus sp. BH72 hypothetical protein YP_932323.1 874352 R 62928 CDS YP_932324.1 119897111 4608361 complement(875655..877499) 1 NC_008702.1 catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using GTP; phosphoenolpyruvate carboxykinase 877499 pckG 4608361 pckG Azoarcus sp. BH72 phosphoenolpyruvate carboxykinase YP_932324.1 875655 R 62928 CDS YP_932325.1 119897112 4609509 877831..880107 1 NC_008702.1 NAD-dependent; catalyzes the oxidative decarboxylation of malate to form pyruvate; does not decarboxylate oxaloacetate; malic enzyme 880107 maeB1 4609509 maeB1 Azoarcus sp. BH72 malic enzyme YP_932325.1 877831 D 62928 CDS YP_932326.1 119897113 4609192 880162..881298 1 NC_008702.1 Probable Hypothetical UPF0028 family protein YML059C. TREMBL:Q82SM1: 50% identity, 68% similariry InterPro:IPR002641; Patatin. Pfam:PF01734; Patatin bioF: 8-amino-7-oxononanoate synthase Signal peptide present (SinalP predicted) TMHMM predicted TMH's; Function unclear; hypothetical protein 881298 4609192 azo0822 Azoarcus sp. BH72 hypothetical protein YP_932326.1 880162 D 62928 CDS YP_932327.1 119897114 4606590 881699..882541 1 NC_008702.1 Penicillin-binding protein 5 precursor (PBP-5) (D-alanyl-D-alanine- endopeptidase) (DD-endopeptidase).; High confidence in function and specificity; D-alanyl-D-alanine carboxypeptidase 882541 pbpG 4606590 pbpG Azoarcus sp. BH72 D-alanyl-D-alanine carboxypeptidase YP_932327.1 881699 D 62928 CDS YP_932328.1 119897115 4609503 complement(882604..883407) 1 NC_008702.1 Probable negative regulator of allantoin and glyoxylate utilization operons,; High confidence in function and specificity; negative regulator of allantoin and glyoxylate utilization operons 883407 allR 4609503 allR Azoarcus sp. BH72 negative regulator of allantoin and glyoxylate utilization operons YP_932328.1 882604 R 62928 CDS YP_932329.1 119897116 4608305 883506..883727 1 NC_008702.1 Hypothetical protein. No homology to the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 883727 4608305 azo0825 Azoarcus sp. BH72 hypothetical protein YP_932329.1 883506 D 62928 CDS YP_932330.1 119897117 4606591 883724..884674 1 NC_008702.1 Glycerate dehydrogenase (EC 1.1.1.29) (NADH-dependent hydroxypyruvate reductase) (HPR) (GDH) (Hydroxypyruvate dehydrogenase) (Glyoxylate reductase) (HPR-A). PLAYS A CENTRAL ROLE IN ASSIMILATION OF CARBON. IT CONVERTS HYDROXYPYRUVATE TO GLYCERATE AS A KEY STEP IN THE SERINE CYCLE AND MAY ALSO PLAY AN IMPORTANT ROLE IN C2 REACTIONS BY INTERCONVERTING GLYOXYLATE AND GLYCOLATE.; Specificity unclear; glycerate dehydrogenase 884674 4606591 azo0826 Azoarcus sp. BH72 glycerate dehydrogenase YP_932330.1 883724 D 62928 CDS YP_932331.1 119897118 4606592 884876..886135 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ORF428 of Roseateles depolymerans of 64% (tremble:BAB1967) Has PF07399:(IPR009978)Protein of unknown function (DUF1504); This family consists of several hypothetical bacterial proteins of around 440 residues in length. The function of this family is unknown.; Conserved hypothetical protein; hypothetical protein 886135 4606592 azo0827 Azoarcus sp. BH72 hypothetical protein YP_932331.1 884876 D 62928 CDS YP_932332.1 119897119 4606593 886288..887790 1 NC_008702.1 Putative RNA pseudouridylate synthase. Homology with hits in the database only for the first half of protein. Has PF00849, RNA pseudouridylate synthase; IPR006145, PseudoU_synth; Members of this family are involved in modifying bases in RNA molecules. They carry out the conversion of uracil bases to pseudouridine. This family includes RluD P33643, a pseudouridylate synthase that converts specific uracils to pseudouridine in 23S rRNA. RluA from E. coli converts bases in both rRNA and tRNA. Has SMART;SM00363, S4 RNA-binding domain;IPR002942;The S4 domain is a small domain consisting of 60-65 amino acid residues that was detected in the bacterial ribosomal protein S4, eukaryotic ribosomal S9, two families of pseudouridine synthases, a novel family of predicted RNA methylases, a yeast protein containing a pseudouridine synthetase and a deaminase domain, bacterial tyrosyl-tRNA synthetases, and a number of uncharacterized, small proteins that may be involved in translation regulation. The S4 domain probably mediates binding to RNA.; Family membership; pseudouridylate synthase 887790 4606593 azo0828 Azoarcus sp. BH72 pseudouridylate synthase YP_932332.1 886288 D 62928 CDS YP_932333.1 119897120 4606594 complement(887858..888337) 1 NC_008702.1 Conderved hypothetical NADH-quinone oxidoreductase chain B. Homology to nuoB of C. violaceum of 77% (trembl|Q7NUE5). NDH-1 shuttles electrons from NADH via FMN and iron- sulfur (Fe-S) centers to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred four hydrogen ions are translocated across the cytoplasmic membrane) and thus conserves the redox energy in a proton gradient (By similarity). Pfam: NADH ubiquinone oxidoreductase, 20 kd no signal peptide no TMHs; Family membership; NADH-quinone oxidoreductase subunit B 888337 nuoB1 4606594 nuoB1 Azoarcus sp. BH72 NADH-quinone oxidoreductase subunit B YP_932333.1 887858 R 62928 CDS YP_932334.1 119897121 4609419 complement(888370..889254) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Conserved hypothetical protein; AraC family transcriptional regulator 889254 4609419 azo0830 Azoarcus sp. BH72 AraC family transcriptional regulator YP_932334.1 888370 R 62928 CDS YP_932335.1 119897122 4606595 complement(889332..889562) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03000467 of Dechloromonas aromatica of 50% (gi|41725519|ref|ZP_00152277.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 889562 4606595 azo0831 Azoarcus sp. BH72 hypothetical protein YP_932335.1 889332 R 62928 CDS YP_932336.1 119897123 4606596 complement(890060..891259) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q8DJ46 (29% identity); TREMBL:Q8YZK5 (29% identity). InterPro (IPR003838): Protein of unknown function DUF214. Pfam (DUF214): Predicted permease. TMHMM reporting four transmembrane helices. TC (3.A.1.122.): The Macrolide Exporter (MacB) Family.; Specificity unclear; ABC transporter permease 891259 4606596 azo0832 Azoarcus sp. BH72 ABC transporter permease YP_932336.1 890060 R 62928 CDS YP_932337.1 119897124 4606597 complement(891269..892477) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q8YZK5 (28% identity); TREMBL:Q8DJ46 (29% identity). Pfam (DUF214): Predicted permease. TMHMM reporting five transmembrane helices. TC (3.A.1.122.): The Macrolide Exporter (MacB) Family.; Specificity unclear; ABC transporter permease 892477 4606597 azo0833 Azoarcus sp. BH72 ABC transporter permease YP_932337.1 891269 R 62928 CDS YP_932338.1 119897125 4606598 complement(892474..893181) 1 NC_008702.1 Putative efflux transporter for macrolide antibiotics (MacB-family). Acts in conjunction with MacA. Similar to TREMBL:Q82W68 (59% identity); TREMBL:Q8RAL4 (46% identity); SWISSPROT:P75831 (41% identity). InterPro (IPR003593): AAA ATPase. InterPro (IPR003439): ABC transporter. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). Pfam (PF00005): ABC transporter. TC (3.A.1.122): The Macrolide Exporter (MacB) Family.; Family membership; ABC transporter ATP-binding protein 893181 4606598 azo0834 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932338.1 892474 R 62928 CDS YP_932339.1 119897126 4606599 complement(893195..894343) 1 NC_008702.1 The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family,; Family membership; membrane fusion protein 894343 4606599 azo0835 Azoarcus sp. BH72 membrane fusion protein YP_932339.1 893195 R 62928 CDS YP_932340.1 119897127 4606600 complement(894555..896288) 1 NC_008702.1 catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; glutaminyl-tRNA synthetase 896288 glnS 4606600 glnS Azoarcus sp. BH72 glutaminyl-tRNA synthetase YP_932340.1 894555 R 62928 CDS YP_932341.1 119897128 4608904 896453..896836 1 NC_008702.1 may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling; camphor resistance protein CrcB 896836 4608904 azo0837 Azoarcus sp. BH72 camphor resistance protein CrcB YP_932341.1 896453 D 62928 CDS YP_932342.1 119897129 4606601 896956..897630 1 NC_008702.1 Conserved hypothetical puter membrane protein Homology to cv1891 of c. violaceum of 64% (trembl|Q7NWT8) InterPro: Bacterial outer membrane protein (IPR006664) Pfam: OmpA family signal peptide 2 TMHs; Family membership; outer membrane protein 897630 4606601 azo0838 Azoarcus sp. BH72 outer membrane protein YP_932342.1 896956 D 62928 CDS YP_932343.1 119897130 4606602 complement(897685..900981) 1 NC_008702.1 SWI/SNF family helicase Pfam: Helicase conserved C-terminal domain; Specificity unclear; SWI/SNF family helicase 900981 4606602 azo0839 Azoarcus sp. BH72 SWI/SNF family helicase YP_932343.1 897685 R 62928 CDS YP_932344.1 119897131 4606603 complement(901163..903079) 1 NC_008702.1 required for the synthesis of the hydromethylpyrimidine moiety of thiamine; thiamine biosynthesis protein ThiC 903079 thiC 4606603 thiC Azoarcus sp. BH72 thiamine biosynthesis protein ThiC YP_932344.1 901163 R 62928 CDS YP_932345.1 119897132 4609934 complement(903169..904023) 1 NC_008702.1 Phosphomethylpyrimidine kinase (EC 2.7.4.7) (HMP-phosphate kinase) (HMP-P kinase). CATALYZES THE PHOSPHORYLATION OF HMP-P TO HMP-PP. TIGRFAM: HMP-P_kinase: phosphomethylpyrimidine kinase; High confidence in function and specificity; phosphomethylpyrimidine kinase 904023 thiD1 4609934 thiD1 Azoarcus sp. BH72 phosphomethylpyrimidine kinase YP_932345.1 903169 R 62928 CDS YP_932346.1 119897133 4609935 904277..905893 1 NC_008702.1 Carbonic anhydrase precursor (EC 4.2.1.1) (Carbonate dehydratase).Involved in the reversible hydration of carbon dioxide. 40% Euk_COanhd.IPR000437; Prok_lipoprot_S.IPR006311;Tat. InterPro: belongs to the eukaryotic-type carbonic anhydrase family. Pfam:PF00194; carb_anhydrase; 1. TIGRFAMs:TIGR01409; TAT_signal_seq; 1. Signal peptide present.; High confidence in function and specificity; putative carbonic anhydrase 905893 ecaA 4609935 ecaA Azoarcus sp. BH72 putative carbonic anhydrase YP_932346.1 904277 D 62928 CDS YP_932347.1 119897134 4608661 complement(905906..907309) 1 NC_008702.1 Conserved hypothetical transcriptional regulator. Homology to rsc0989 of r. solanacearum of 47% (CAD14691). Pfam: Bacterial regulatory proteins, gntR family; Aminotransferase class I and II no signal peptide no TMHs bioF: 8-amino-7-oxononanoate synthase; Specificity unclear; putative transcriptional regulator 907309 4608661 azo0843 Azoarcus sp. BH72 putative transcriptional regulator YP_932347.1 905906 R 62928 CDS YP_932348.1 119897135 4606604 907471..907926 1 NC_008702.1 Hypothetical secreted protein. No homology to the data base. No domain predicted. No TMHs. Signal peptide.; hypothetical protein 907926 4606604 azo0844 Azoarcus sp. BH72 hypothetical protein YP_932348.1 907471 D 62928 CDS YP_932349.1 119897136 4606605 908009..908686 1 NC_008702.1 Conserved hypothetical transcriptional regulator. Homology to RSc0698 of alstonia solanacearum of 48% (gnl|keqq|rso:RS01603(KEGG)). InterPro: IPR001647 HTH_TetR. Pfam: PF00440 Bacterial regulatory proteins,tetR family. HTH reporting nucleic acid binding motif. No signal peptide. No TMHs.; Conserved hypothetical protein; putative transcriptional regulator 908686 4606605 azo0845 Azoarcus sp. BH72 putative transcriptional regulator YP_932349.1 908009 D 62928 CDS YP_932350.1 119897137 4606606 908683..909771 1 NC_008702.1 Similar to TREMBL:Q7NR61 (35% identity); TREMBL:Q8XYV3 (33% identity); TREMBL:Q88MQ4 (32% identity). InterPro (IPR006143): Secretion protein HlyD. SignalP predicting signal peptide. TC (2.A.6.1): The Heavy Metal Efflux (HME) Family.; Family membership; membrane fusion protein 909771 4606606 azo0846 Azoarcus sp. BH72 membrane fusion protein YP_932350.1 908683 D 62928 CDS YP_932351.1 119897138 4606607 909768..912986 1 NC_008702.1 AcrB/AcrD/AcrF family. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q9A7D5 (35% identity); TREMBL:Q9HXW4 (55% identity). Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TMHMM predicting 12 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 912986 4606607 azo0847 Azoarcus sp. BH72 RND efflux transporter permease YP_932351.1 909768 D 62928 CDS YP_932352.1 119897139 4606608 913062..913715 1 NC_008702.1 Putative protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins (By similarity). InterPro: Protein-L-isoaspartate(D-aspartate) O-methyltransferase (IPR000682), SAM binding motif (IPR000051) Tigrfam: pimt: protein-L-isoaspartate O-methyltransfase Pfam: Protein-L-isoaspartate (D-aspartate) O-methyltransferase no signal peptide no TMHs; High confidence in function and specificity; putative protein-L-isoaspartate O-methyltransferase 913715 pcm1 4606608 pcm1 Azoarcus sp. BH72 putative protein-L-isoaspartate O-methyltransferase YP_932352.1 913062 D 62928 CDS YP_932353.1 119897140 4609510 913724..914047 1 NC_008702.1 Putative rhodanese-related sulfurtransferase. TIGR:NMB1884. InterPro:IPR001763; Rhodanese-like. InterPro: Rhodanese/cdc25 fold. Pfam:PF00581; Rhodanese; 1.; Function unclear; putative sulfurtransferase 914047 4609510 azo0849 Azoarcus sp. BH72 putative sulfurtransferase YP_932353.1 913724 D 62928 CDS YP_932354.1 119897141 4606609 914066..915382 1 NC_008702.1 Putative outer membrane effluxe protein protein. Homology to tolC from E. coli of 33%. The OEP family (outer membreane effluxe protein) allow export of a variety of substrates in Gram negative bacteria. TolC is believed to be a membre af an ABC transporter system for protein secretion without cleavage of a signal sequence. Pfam: Outer membrane efflux protein Signal peptide no TMHs; Family membership; putative outer membrane efflux protein 915382 tolC 4606609 tolC Azoarcus sp. BH72 putative outer membrane efflux protein YP_932354.1 914066 D 62928 CDS YP_932355.1 119897142 4609959 complement(915379..916623) 1 NC_008702.1 catalyzes the transfer of 2-keto-3-deoxy-D-manno-octulosonic acid to lipid A; 3-deoxy-D-manno-octulosonic-acid transferase 916623 waaA 4609959 waaA Azoarcus sp. BH72 3-deoxy-D-manno-octulosonic-acid transferase YP_932355.1 915379 R 62928 CDS YP_932356.1 119897143 4610100 916809..917816 1 NC_008702.1 Probably involved in the biosynthesis of exopolysaccharides. 68% Epimerase_Dh.IPR008089; Nuc_sugar_epim. Pfam:PF01370; Epimerase; 1.; High confidence in function and specificity; putative nucleotide sugar epimerase 917816 wbnF 4610100 wbnF Azoarcus sp. BH72 putative nucleotide sugar epimerase YP_932356.1 916809 D 62928 CDS YP_932357.1 119897144 4610110 917838..918635 1 NC_008702.1 hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP; diadenosine tetraphosphatase 918635 apaH 4610110 apaH Azoarcus sp. BH72 diadenosine tetraphosphatase YP_932357.1 917838 D 62928 CDS YP_932358.1 119897145 4608324 complement(918645..919436) 1 NC_008702.1 Function:-Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position (By similarity). CATALYTIC ACTIVITY: Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Entry name:- PLSC_NEIGO Primary accession number :- Q59601 InterPro IPR002123; Acyltransferase. IPR004552; AGP_acyltrn. Pfam PF01553; Acyltransferase; 1. Identities = 68/182 (37%) Prediction: Non-secretory protein Signal peptide probability: 0.096 Number of predicted TMHs: 0; Family membership; putative 1-acyl-sn-glycerol-3-phosphate acyltransferase 919436 plsC 4608324 plsC Azoarcus sp. BH72 putative 1-acyl-sn-glycerol-3-phosphate acyltransferase YP_932358.1 918645 R 62928 CDS YP_932359.1 119897146 4609584 complement(919508..920266) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv0660 of C. violaceum of 59% (trembl|Q7P0A7) no domains predicted no singal peptide no TMHs; hypothetical protein 920266 4609584 azo0855 Azoarcus sp. BH72 hypothetical protein YP_932359.1 919508 R 62928 CDS YP_932360.1 119897147 4606610 920383..921654 1 NC_008702.1 Similar to amylovoran biosynthesis glycosyl transferase amsK (EC 2.-.-.-). INVOLVED IN THE BIOSYNTHESIS OF AMYLOVORAN WHICH FUNCTIONS AS A VIRULENCE FACTOR. Similar to protein annotated as sulfolipid sulfoquinovosyldiacylglycerol biosynthesis proteins.; Family membership; glycosyltransferase 921654 4606610 azo0856 Azoarcus sp. BH72 glycosyltransferase YP_932360.1 920383 D 62928 CDS YP_932361.1 119897148 4606611 921718..922653 1 NC_008702.1 38% PfkB.Family of carbohydrate kinases Pfam:PF00294; PfkB; 1.; Specificity unclear; putative carbohydrate kinase 922653 4606611 azo0857 Azoarcus sp. BH72 putative carbohydrate kinase YP_932361.1 921718 D 62928 CDS YP_932362.1 119897149 4606612 922647..923120 1 NC_008702.1 Outer membrane lipoprotein SlyB. Homology to slyB of E. coli of 32% (sprot|SLYB_ECOLI) no domains predicted signal peptide no TMHs; Family membership; putative outer membrane protein SlyB 923120 slyB 4606612 slyB Azoarcus sp. BH72 putative outer membrane protein SlyB YP_932362.1 922647 D 62928 CDS YP_932363.1 119897150 4609875 complement(923122..923514) 1 NC_008702.1 Probable Diacylglycerol kinase. Homology to dgkA of E. coli of 40% (sprot|KDGL_ECOLI) RECYCLING OF DIACYLGLYCEROL PRODUCED DURING THE TURNOVER OF MEMBRANE PHOSPHOLIPID (BY SIMILARITY). Pfam: prokaryotic diacylglycerol kinase no signal peptide 2 TMHs; High confidence in function and specificity; diacylglycerol kinase 923514 dgkA 4609875 dgkA Azoarcus sp. BH72 diacylglycerol kinase YP_932363.1 923122 R 62928 CDS YP_932364.1 119897151 4608620 complement(923623..924912) 1 NC_008702.1 Hypothetical protein. No Domains,Features,Signal Pepetide or TMH reported Present. Most top hits suggest the possibillity of Transmembrane protein, but due to non existance of any TMH's domains it cant be considered as a choice.; Specificity unclear; hypothetical protein 924912 4608620 azo0860 Azoarcus sp. BH72 hypothetical protein YP_932364.1 923623 R 62928 CDS YP_932365.1 119897152 4606613 complement(924919..925803) 1 NC_008702.1 methylates ribosomal protein L11 at multiple amino acid positions; mutations of these genes in Escherichia coli or Thermus thermophilus has no apparent phenotype; 50S ribosomal protein L11 methyltransferase 925803 prmA 4606613 prmA Azoarcus sp. BH72 50S ribosomal protein L11 methyltransferase YP_932365.1 924919 R 62928 CDS YP_932366.1 119897153 4609631 complement(925814..927175) 1 NC_008702.1 an AccC homodimer forms the biotin carboxylase subunit of the acetyl CoA carboxylase, an enzyme that catalyzes the formation of malonyl-CoA, which in turn controls the rate of fatty acid metabolism; acetyl-CoA carboxylase biotin carboxylase subunit 927175 accC1 4609631 accC1 Azoarcus sp. BH72 acetyl-CoA carboxylase biotin carboxylase subunit YP_932366.1 925814 R 62928 CDS YP_932367.1 119897154 4608256 complement(927168..927296) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 927296 4608256 azo0863 Azoarcus sp. BH72 hypothetical protein YP_932367.1 927168 R 62928 CDS YP_932368.1 119897155 4606614 complement(927305..927757) 1 NC_008702.1 Function:-Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP). THIS PROTEIN IS A COMPONENT OF THE ACETYL COENZYME A CARBOXYLASE COMPLEX; FIRST BIOTIN CARBOXYLASE CATALYZES THE CARBOXYLATION OF THE CARRIER PROTEIN AND THEN THE TRANSCARBOXYLASE TRANSFERS THE CARBOXYL GROUP TO FORM MALONYL-COA. Entry name:-SWISSPROT:BCCP_ECOLI Prim. accession # P02905 InterPro :-IPR001249; AcCoA_biotinCC. IPR001882; Biotin_BS. IPR000089; Biotin_lipoyl. Pfam:- PF00364; Biotin_lipoyl; 1. Number of predicted TMHs: 0 Identity:- 94/157 (59%); High confidence in function and specificity; biotin carboxyl carrier protein of acetyl-CoA carboxylase 927757 accB 4606614 accB Azoarcus sp. BH72 biotin carboxyl carrier protein of acetyl-CoA carboxylase YP_932368.1 927305 R 62928 CDS YP_932369.1 119897156 4608255 complement(927813..928310) 1 NC_008702.1 catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis; 3-dehydroquinate dehydratase 928310 aroQ 4608255 aroQ Azoarcus sp. BH72 3-dehydroquinate dehydratase YP_932369.1 927813 R 62928 CDS YP_932370.1 119897157 4608349 complement(928372..928908) 1 NC_008702.1 Hypothetical thioredoxin. Homology with BPP3919 of B. parapertussis of 38%. Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. Tigrfam: dsbE: periplasmic protein thiol:disulfi signal peptide probable 1 TMHs; Family membership; putative thioredoxin 928908 4608349 azo0866 Azoarcus sp. BH72 putative thioredoxin YP_932370.1 928372 R 62928 CDS YP_932371.1 119897158 4606615 complement(928905..929594) 1 NC_008702.1 Conserved hypothetical protein, 39% identity to TrEMBL;Q8XVP7; Signal Peptide present. Coils2 program reporting presence of coiled coil. No TMH present. TIGR00004: endoribonuclease L-PSP putat; hypothetical protein 929594 4606615 azo0867 Azoarcus sp. BH72 hypothetical protein YP_932371.1 928905 R 62928 CDS YP_932372.1 119897159 4606616 929650..930996 1 NC_008702.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso- diaminopimelate ligase (EC 6.3.2.-) (Murein peptide ligase). REUTILIZES THE INTACT TRIPEPTIDE L-ALANYL-GAMMA-D- GLUTAMYL-MESO-DIAMINOPIMELATE BY LINKING IT TO UDP-N-ACETYLMURAMIC ACID. InterPro: Cytoplasmic peptidoglycan synthetases N-terminal ispD: 4-diphosphocytidyl-2C-methyl-D-er; High confidence in function and specificity; UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase 930996 mpl 4606616 mpl Azoarcus sp. BH72 UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase YP_932372.1 929650 D 62928 CDS YP_932373.1 119897160 4609267 complement(931018..931668) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc3264 of R. solanacearum (trembl|Q8XUC7). Smart: Bacterial OsmY and nodulation domain (BON) The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins; outside of this group the distribution is more disparate. signal peptide TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 931668 4609267 azo0869 Azoarcus sp. BH72 hypothetical protein YP_932373.1 931018 R 62928 CDS YP_932374.1 119897161 4606617 complement(931665..932258) 1 NC_008702.1 catalyzes the isomerization of sedoheptulose 7-phosphate to D-glycero-D-manno-heptose 7-phosphate; phosphoheptose isomerase 932258 gmhA 4606617 gmhA Azoarcus sp. BH72 phosphoheptose isomerase YP_932374.1 931665 R 62928 CDS YP_932375.1 119897162 4608927 complement(932374..932787) 1 NC_008702.1 Conserved hypothetical protein. Homology to pa4424 of P. aeruginosa of 44% (pir|H83091). Pfam: Uncharacterised protein family UPF0102 (PF02021). Interpro: Protein of unkown function UPF0102 (IPR003509). no signal peptide. no TMHs.; hypothetical protein 932787 4608927 azo0871 Azoarcus sp. BH72 hypothetical protein YP_932375.1 932374 R 62928 CDS YP_932376.1 119897163 4606618 932786..933673 1 NC_008702.1 Conserved hypothetical protein. Homology to pp1326 of P. putida of 56% ((AAN66950). InterPro: Uncharacterized protein family UPF0011 (IPR008189) TIGR00096: conserved hypothetical protein Pfam: Tetropyrrole (Crrin/Porphyrin) methyltransferase no signal peptide no TMHs; hypothetical protein 933673 4606618 azo0872 Azoarcus sp. BH72 hypothetical protein YP_932376.1 932786 D 62928 CDS YP_932377.1 119897164 4606619 933728..934762 1 NC_008702.1 catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis; dihydroorotase 934762 pyrC 4606619 pyrC Azoarcus sp. BH72 dihydroorotase YP_932377.1 933728 D 62928 CDS YP_932378.1 119897165 4609669 934759..935190 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03003273 of Dechloromonas aromatica of 35% (gi|41723286|ref|ZP_00150229.1|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 935190 4609669 azo0874 Azoarcus sp. BH72 hypothetical protein YP_932378.1 934759 D 62928 CDS YP_932379.1 119897166 4606620 935993..936436 1 NC_008702.1 MraZ; UPF0040; crystal structure shows similarity to AbrB; cell division protein MraZ 936436 mraZ 4606620 mraZ Azoarcus sp. BH72 cell division protein MraZ YP_932379.1 935993 D 62928 CDS YP_932380.1 119897167 4609270 936433..937371 1 NC_008702.1 S-adenosyl-methyltransferase mraW (EC 2.1.1.-). Exhibits a S-adenosyl-dependent methyltransferase activity (By similarity). TIGR00006: conserved hypothetical prote; High confidence in function and specificity; S-adenosyl-methyltransferase MraW 937371 mraW 4609270 mraW Azoarcus sp. BH72 S-adenosyl-methyltransferase MraW YP_932380.1 936433 D 62928 CDS YP_932381.1 119897168 4609268 937368..937631 1 NC_008702.1 Putative cell division protein FtsL. Homology to ftsL of E. coli of 32% (sprot|FTSL_ECOLI). Protein involved in cell division and cell growth. May play some role in coupling cell division and peptidoglycan physiology. Pfam: Cell division protein FtsL signal peptide no TMHs; Family membership; putative cell division protein FtsL 937631 FtsL 4609268 FtsL Azoarcus sp. BH72 putative cell division protein FtsL YP_932381.1 937368 D 62928 CDS YP_932382.1 119897169 4608838 937628..939388 1 NC_008702.1 Probable peptidoglycan glycosyltransferase. Homology to ftsI of E. coli of 43% (sprot|FTSI_ECOLI) Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates. InterPro: Penicillin binding protein transpeptidase domain (IPR001460) Pfam: Penicillin-binding protein dimerisation domain; Penicillin binding proetin transpeptidas domain signal peptide no TMHs; High confidence in function and specificity; peptidoglycan glycosyltransferase 939388 FtsI 4608838 FtsI Azoarcus sp. BH72 peptidoglycan glycosyltransferase YP_932382.1 937628 D 62928 CDS YP_932383.1 119897170 4608835 939385..940890 1 NC_008702.1 Probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase Homology to murE of E. coli of 40% (sprot|MURE_ECOLI) Cell wall formation. Diaminopimelic acid adding enzyme (By similarity). Pfam: Mur ligase family, catalytic domain; Mur ligase family, glutamate ligase no signal peptide no TMHs; High confidence in function and specificity; UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase 940890 murE 4608835 murE Azoarcus sp. BH72 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase YP_932383.1 939385 D 62928 CDS YP_932384.1 119897171 4609291 940863..942299 1 NC_008702.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (EC 6.3.2.10) (UDP-MurNAc-pentapeptide synthetase) (D-alanyl-D- alanine-adding enzyme). Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide the precursor of murein (By similarity). InterPro: Cytoplasmic peptidoglycan synthetases N-terminal mobB: molybdopterin-guanine dinucleot; High confidence in function and specificity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase 942299 murF 4609291 murF Azoarcus sp. BH72 UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase YP_932384.1 940863 D 62928 CDS YP_932385.1 119897172 4609292 942301..943389 1 NC_008702.1 First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; phospho-N-acetylmuramoyl-pentapeptide- transferase 943389 mraY 4609292 mraY Azoarcus sp. BH72 phospho-N-acetylmuramoyl-pentapeptide- transferase YP_932385.1 942301 D 62928 CDS YP_932386.1 119897173 4609269 943386..944804 1 NC_008702.1 UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase 944804 murD 4609269 murD Azoarcus sp. BH72 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase YP_932386.1 943386 D 62928 CDS YP_932387.1 119897174 4609290 944801..946033 1 NC_008702.1 This is a septum-peptidoglycan biosynthetic protein involved in cell wall formation. Plays a role in the stabilization of the ftsZ ring during cell division.; High confidence in function and specificity; cell division protein FtsW 946033 FtsW 4609290 FtsW Azoarcus sp. BH72 cell division protein FtsW YP_932387.1 944801 D 62928 CDS YP_932388.1 119897175 4608840 946030..947100 1 NC_008702.1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis; undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase 947100 murG 4608840 murG Azoarcus sp. BH72 undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase YP_932388.1 946030 D 62928 CDS YP_932389.1 119897176 4609293 947097..948500 1 NC_008702.1 Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis; UDP-N-acetylmuramate--L-alanine ligase 948500 murC 4609293 murC Azoarcus sp. BH72 UDP-N-acetylmuramate--L-alanine ligase YP_932389.1 947097 D 62928 CDS YP_932390.1 119897177 4609289 948497..949411 1 NC_008702.1 D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanine--D-alanine ligase 949411 ddl 4609289 ddl Azoarcus sp. BH72 D-alanine--D-alanine ligase YP_932390.1 948497 D 62928 CDS YP_932391.1 119897178 4608613 949404..950234 1 NC_008702.1 Putative cell division protein FtsQ. Homology to ftsQ of E. coli of 23% (Sprot:FTSQ_ECOLI) This protein may be involved in septum formation. Pfam: Cell division protein FtsQ no signal peptide 1 TMHs; Family membership; putative cell division protein FtsQ 950234 FtsQ 4608613 FtsQ Azoarcus sp. BH72 putative cell division protein FtsQ YP_932391.1 949404 D 62928 CDS YP_932392.1 119897179 4608839 950231..951460 1 NC_008702.1 This protein may be involved in anomalous filament growth. May be a component of the septum. It may interact with ftsZ. InterPro: Cell division protein FtsA; High confidence in function and specificity; cell division protein FtsA 951460 FtsA 4608839 FtsA Azoarcus sp. BH72 cell division protein FtsA YP_932392.1 950231 D 62928 CDS YP_932393.1 119897180 4608832 951602..952744 1 NC_008702.1 GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function; cell division protein FtsZ 952744 FtsZ 4608832 FtsZ Azoarcus sp. BH72 cell division protein FtsZ YP_932393.1 951602 D 62928 CDS YP_932394.1 119897181 4608843 952910..953833 1 NC_008702.1 zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase 953833 lpxC 4608843 lpxC Azoarcus sp. BH72 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase YP_932394.1 952910 D 62928 CDS YP_932395.1 119897182 4609176 953868..954056 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. signal peptide. 1 TMH; hypothetical protein 954056 4609176 azo0891 Azoarcus sp. BH72 hypothetical protein YP_932395.1 953868 D 62928 CDS YP_932396.1 119897183 4606621 complement(954061..954489) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV4285 of C.violaceum of 32% (tremble:Q7NQ55). No domains predicted. No TMHs No signal peptide.; hypothetical protein 954489 4606621 azo0892 Azoarcus sp. BH72 hypothetical protein YP_932396.1 954061 R 62928 CDS YP_932397.1 119897184 4606622 954595..957324 1 NC_008702.1 functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA 957324 secA 4606622 secA Azoarcus sp. BH72 preprotein translocase subunit SecA YP_932397.1 954595 D 62928 CDS YP_932398.1 119897185 4609857 957401..958882 1 NC_008702.1 GAF-domain containing protein,; Conserved hypothetical protein; GAF-domain-containing protein 958882 4609857 azo0894 Azoarcus sp. BH72 GAF-domain-containing protein YP_932398.1 957401 D 62928 CDS YP_932399.1 119897186 4606623 958968..960209 1 NC_008702.1 bifunctional arginine biosynthesis protein ArgJ; functions at the 1st and 5th steps in arginine biosynthesis; involved in synthesis of acetylglutamate from glutamate and acetyl-CoA and ornithine by transacetylation between acetylornithine and glutmate; bifunctional ornithine acetyltransferase/N-acetylglutamate synthase 960209 argJ 4606623 argJ Azoarcus sp. BH72 bifunctional ornithine acetyltransferase/N-acetylglutamate synthase YP_932399.1 958968 D 62928 CDS YP_932400.1 119897187 4608338 960324..961154 1 NC_008702.1 Putative phosphoprotein phosphatase,; Conserved hypothetical protein; putative phosphoprotein phosphatase 961154 4608338 azo0896 Azoarcus sp. BH72 putative phosphoprotein phosphatase YP_932400.1 960324 D 62928 CDS YP_932401.1 119897188 4606624 961167..962186 1 NC_008702.1 Hypothetical secreted protein. Homology to pp3428 of P. putida of 26% (tremble:Q88HD2). Domain structure: 111 aa - 157 aa LysM; 232 aa - 313 aa TRP. Pfam: LysM domain; TRP. signal peptide. no TMHs; hypothetical protein 962186 4606624 azo0897 Azoarcus sp. BH72 hypothetical protein YP_932401.1 961167 D 62928 CDS YP_932402.1 119897189 4606625 complement(962191..963855) 1 NC_008702.1 Putative serine/threonine protein kinase,; Family membership; putative serine/threonine protein kinase 963855 4606625 azo0898 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_932402.1 962191 R 62928 CDS YP_932403.1 119897190 4606626 complement(963863..964384) 1 NC_008702.1 Hypothetical membrane protein. No good homology to a protein of similar size of the data bank. Pfam: FHA domain (PF00498). The forkhead-associated (FHA) domain is a putative nuclear signalling domain found in a variety of otherwise unrelated proteins. The FHA domain comprise approximately 55 to 75 amino acids and contains three highly conserved blocks separated by divergent spacer regions.To date, genes encoding FHA-containing proteins have been identified in eubacterial and eukaryotic but not archaeal genomes. The domain is present in a diverse range of proteins, such as kinases, phosphatases, kinesins,transcription factors, RNA-binding proteins and metabolic enzymes which partake in many different cellular processes - DNA repair, signal transduction, vesicular transport and protein degradation are just a few examples. Interpro: Forkhead-associated (IPR000253). no signal peptide. 1 TMHs; hypothetical protein 964384 4606626 azo0899 Azoarcus sp. BH72 hypothetical protein YP_932403.1 963863 R 62928 CDS YP_932404.1 119897191 4606627 complement(964488..964871) 1 NC_008702.1 protein associated with Co2+ and Mg2+ efflux; ApaG protein 964871 apaG 4606627 apaG Azoarcus sp. BH72 ApaG protein YP_932404.1 964488 R 62928 CDS YP_932405.1 119897192 4608323 965057..966433 1 NC_008702.1 Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; adenylosuccinate lyase 966433 purB 4608323 purB Azoarcus sp. BH72 adenylosuccinate lyase YP_932405.1 965057 D 62928 CDS YP_932406.1 119897193 4609655 966512..966829 1 NC_008702.1 Conserved hypothetical protein. Homology to RS03757 of R.solanacearum of 44% (tremble:Q8XS71). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 966829 4609655 azo0902 Azoarcus sp. BH72 hypothetical protein YP_932406.1 966512 D 62928 CDS YP_932407.1 119897194 4606628 complement(966923..967855) 1 NC_008702.1 forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF; preprotein translocase subunit SecF 967855 secF 4606628 secF Azoarcus sp. BH72 preprotein translocase subunit SecF YP_932407.1 966923 R 62928 CDS YP_932408.1 119897195 4609861 complement(967876..969750) 1 NC_008702.1 part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; preprotein translocase subunit SecD 969750 secD 4609861 secD Azoarcus sp. BH72 preprotein translocase subunit SecD YP_932408.1 967876 R 62928 CDS YP_932409.1 119897196 4609859 complement(969851..970180) 1 NC_008702.1 member of preprotein translocase; forms a heterotrimer with SecD and SecF; links the SecD/SecF/YajC/YidC complex with the SecY/SecE/SecG complex; preprotein translocase subunit YajC 970180 yajC 4609859 yajC Azoarcus sp. BH72 preprotein translocase subunit YajC YP_932409.1 969851 R 62928 CDS YP_932410.1 119897197 4610141 970362..971201 1 NC_008702.1 Conserved hypothetical signal transduction protein. Homology to Daro03002984 of Dechloromonas aromatica of 39% (gi|53729872|ref|ZP_00150342.2|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs; Conserved hypothetical protein; putative signal transduction protein 971201 4610141 azo0906 Azoarcus sp. BH72 putative signal transduction protein YP_932410.1 970362 D 62928 CDS YP_932411.1 119897198 4606629 complement(971214..972326) 1 NC_008702.1 Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr); queuine tRNA-ribosyltransferase 972326 tgt 4606629 tgt Azoarcus sp. BH72 queuine tRNA-ribosyltransferase YP_932411.1 971214 R 62928 CDS YP_932412.1 119897199 4609932 complement(972316..973353) 1 NC_008702.1 Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step; S-adenosylmethionine--tRNA ribosyltransferase-isomerase 973353 queA 4609932 queA Azoarcus sp. BH72 S-adenosylmethionine--tRNA ribosyltransferase-isomerase YP_932412.1 972316 R 62928 CDS YP_932413.1 119897200 4610002 complement(973554..974405) 1 NC_008702.1 Transcriptional activator protein nhaR (Na+/H+ antiporter regulatory protein). Plays a role in the positive regulation of nhaA. Similar to SWISSPROT: sprot|NHAR_ECOLI (34% Escherichia coli, and Shigella flexneri, transcriptional activator protein NhaR (na+/h+ antiporter regulatory protein)) InterPro: IPR000847 HTH_LysR. IPR009058 Winged helix DNA-binding. Pfam: PF00126 Bacterial regulatory helix-turn-helix protein,lysR family.; High confidence in function and specificity; LysR family transcriptional regulator 974405 nhaR 4610002 nhaR Azoarcus sp. BH72 LysR family transcriptional regulator YP_932413.1 973554 R 62928 CDS YP_932414.1 119897201 4609355 974584..974925 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD81 Azoarcus sp. EbN1 of 64% (gnl|keqq|eba:ebD81(KEGG)). No domains predicted. No signal peptid. No TMHs; hypothetical protein 974925 4609355 azo0910 Azoarcus sp. BH72 hypothetical protein YP_932414.1 974584 D 62928 CDS YP_932415.1 119897202 4606630 974987..975652 1 NC_008702.1 Hypothetical protein PA2604. pir:D85624: 57% identity.73% similarity SIMILARITY:Belongs to the BI1 family. InterPro:Uncharacterized protein family UPF0005 IPR006213; Bax_inhbtr1. IPR006214; UPF0005. Pfam:ZIP: Zinc transporter Probable glutamatereceptor Signal peptide present (SignalP predicted) and transmembrane helices 7 (TMHMM predicted) Pfam:PF01027; UPF0005; InterPro: Uncharacterized protein family UPF0005 2_A_01_02: Multidrug resistance protein; Specificity unclear; carrier/transport protein 975652 yccA 4606630 yccA Azoarcus sp. BH72 carrier/transport protein YP_932415.1 974987 D 62928 CDS YP_932416.1 119897203 4610155 975796..977517 1 NC_008702.1 Probable potassium antiporter, rosB. 38% HPr_SerP_S.IPR004771; K_eff.IPR006153; Na_H_porter. IPR003148; TrkA_N. Pfam:PF00999; Na_H_Exchanger; 1.PF02254; TrkA_N; 1. TIGRFAMs:TIGR00932; 2a37; 1. TMhelix: 12.; High confidence in function and specificity; putative potassium/proton antiporter 977517 rosB 4610155 rosB Azoarcus sp. BH72 putative potassium/proton antiporter YP_932416.1 975796 D 62928 CDS YP_932417.1 119897204 4609757 complement(977524..978858) 1 NC_008702.1 Hypothetical protein sll0260. CBS domains are found in the intracellular regions of a number of different integral membrane proteins. Two CBS domains are found in intracellular loops of several voltage gated chloride channels. A family of magnesium transporters also contain CBS domains. TREMBL:Q8KEZ1: 61% identity, 74% similarity InterPro: Domain of unknown function DUF21 InterPro:IPR002550; CBS. IPR000644: CBS_domain. IPR005170: CorC_transpt-asc. Pfam: PF00571; CBS; 2. PF03471: CorC_HlyC; 1. PF01595: DUF21; gntP: gluconate transporter Signal peptide present Transmembrane helices 3; Specificity unclear; hemolysin 978858 ytfL2 4609757 ytfL2 Azoarcus sp. BH72 hemolysin YP_932417.1 977524 R 62928 CDS YP_932418.1 119897205 4610198 979395..980357 1 NC_008702.1 Tellurium resistance protein terC. Could conceivably alter the intracellular level of tellurium in a manner leading to resistance. Alternatively its presence in the membrane may provide a barrier to entry of the tellurium ions.32% TerC. Pfam: PF03741; TerC; 1. TMHelix:9.; High confidence in function and specificity; putative tellurium resistance protein 980357 terC 4610198 terC Azoarcus sp. BH72 putative tellurium resistance protein YP_932418.1 979395 D 62928 CDS YP_932419.1 119897206 4609927 980435..981400 1 NC_008702.1 catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein; acetyl-CoA carboxylase carboxyltransferase subunit alpha 981400 accA 4609927 accA Azoarcus sp. BH72 acetyl-CoA carboxylase carboxyltransferase subunit alpha YP_932419.1 980435 D 62928 CDS YP_932420.1 119897207 4608254 981412..982758 1 NC_008702.1 Putative tRNA(Ile)-lysidine synthetase. Homology to tils of E.coli of 34% (gnl|keqq|eco:b0188(KEGG)). Pfam: PP-loop family. Tigrfam: tRNA(Ile)-lysidine synthetase. The only examples in which the wobble position of a tRNA must discriminate between G and A of mRNA are AUA (Ile) vs. AUG (Met) and UGA (stop) vs. UGG (Trp). In all bacteria, the wobble position of the tRNA(Ile) recognizing AUA is lysidine, a lysine derivative of cytidine. This family describes a protein domain found, apparently, in all bacteria in a single copy. Eukaryotic sequences appear to be organellar. The domain archictecture of this protein family is variable; some, including characterized proteins of E. coli and B. subtilis known to be tRNA(Ile)-lysidine synthetase, include a conserved 50-residue domain that many other members lack. This protein belongs to the ATP-binding PP-loop family ( PF01171). It appears in the literature and protein databases as TilS, YacA, and putative cell cycle protein MesJ (a misnomer). No signal peptide. No TMHs.; Family membership; putative tRNA(Ile)-lysidine synthetase 982758 tilS 4608254 tilS Azoarcus sp. BH72 putative tRNA(Ile)-lysidine synthetase YP_932420.1 981412 D 62928 CDS YP_932421.1 119897208 4609948 complement(982714..983319) 1 NC_008702.1 C4-dicarboxylate transport transcriptional regulatory protein,; High confidence in function and specificity; two-component response regulator 983319 4609948 azo0917 Azoarcus sp. BH72 two-component response regulator YP_932421.1 982714 R 62928 CDS YP_932422.1 119897209 4606631 complement(983320..985311) 1 NC_008702.1 C4-dicarboxylate transport sensor protein,; High confidence in function and specificity; two-component sensor kinase 985311 dctS 4606631 dctS Azoarcus sp. BH72 two-component sensor kinase YP_932422.1 983320 R 62928 CDS YP_932423.1 119897210 4608612 985540..986544 1 NC_008702.1 TRAP-dicarboxylate transporter. Binds c4-dicarboxylates; part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. 70% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide: present. TMhelix:1; High confidence in function and specificity; c4-dicarboxylate-binding periplasmic protein 986544 dctP2 4608612 dctP2 Azoarcus sp. BH72 c4-dicarboxylate-binding periplasmic protein YP_932423.1 985540 D 62928 CDS YP_932424.1 119897211 4608603 986612..987310 1 NC_008702.1 The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 45% DctQ. Pfam:PF04290; DctQ; 1. TMhelix:4.; High confidence in function and specificity; putative TRAP-type C4-dicarboxylate transport system small permease 987310 dctQ2 4608603 dctQ2 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system small permease YP_932424.1 986612 D 62928 CDS YP_932425.1 119897212 4608609 987325..988611 1 NC_008702.1 The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 60% DctM.IPR000252; DedA.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1. TMhelix:13. Signal peptide: present.; High confidence in function and specificity; putative TRAP-type C4-dicarboxylate transport system large permease 988611 dctM2 4608609 dctM2 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system large permease YP_932425.1 987325 D 62928 CDS YP_932426.1 119897213 4608598 988762..989409 1 NC_008702.1 DedA family protein, 57% identity(75% similarity) to TrEMBL;Q88QF4. TrEMBl;Q889M5(57% identity). SwissProt;P09548(55% identity) Has PF00597, DedA family;IPR000252;This family combines the DedA related proteins and YIAN/YGIK family. Members of this family are not functionally characterised. These proteins contain multiple predicted transmembrane regions.; High confidence in function and specificity; DedA protein 989409 dedA 4608598 dedA Azoarcus sp. BH72 DedA protein YP_932426.1 988762 D 62928 CDS YP_932427.1 119897214 4608615 989496..989921 1 NC_008702.1 catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate; nucleoside diphosphate kinase 989921 ndk 4608615 ndk Azoarcus sp. BH72 nucleoside diphosphate kinase YP_932427.1 989496 D 62928 CDS YP_932428.1 119897215 4609350 990009..991136 1 NC_008702.1 Hypothetical protein yfgB. putative fe-s-cluster redox enzyme TREMBL:Q7NS85: 70% identity; 80% similarity. InterPro:IPR004383; Cons_hypoth48. IPR007197: Radical_SAM. Pfam:PF04055; Radical_SAM; No signal peptide. No transmembrane helices. TIGR00048: conserved hypothetical prote; Function unclear; hypothetical protein 991136 4609350 azo0924 Azoarcus sp. BH72 hypothetical protein YP_932428.1 990009 D 62928 CDS YP_932429.1 119897216 4606632 991136..991951 1 NC_008702.1 Type 4 pilus biogenesis protein,; Function unclear; putative type 4 pilus biogenesis protein 991951 pilF 4606632 pilF Azoarcus sp. BH72 putative type 4 pilus biogenesis protein YP_932429.1 991136 D 62928 CDS YP_932430.1 119897217 4609560 991948..992856 1 NC_008702.1 Transcriptional regulator , 36% identity to TrEMBL;Q88PJ8. Weak homology with other proteins spanning entire length. Prosite,PS50943; HTH_CROC1; The cro/C1-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 50-60 residues present in transcriptional regulators. The domain is named after the transcriptional repressors cro and C1 of temperate bacteriophages 434 and lambda, respectively.; Specificity unclear; transcriptional regulator 992856 4609560 azo0926 Azoarcus sp. BH72 transcriptional regulator YP_932430.1 991948 D 62928 CDS YP_932431.1 119897218 4606633 992856..994103 1 NC_008702.1 catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 994103 ispG 4606633 ispG Azoarcus sp. BH72 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase YP_932431.1 992856 D 62928 CDS YP_932432.1 119897219 4608868 994138..995439 1 NC_008702.1 catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG; histidyl-tRNA synthetase 995439 hisS 4608868 hisS Azoarcus sp. BH72 histidyl-tRNA synthetase YP_932432.1 994138 D 62928 CDS YP_932433.1 119897220 4609009 995426..996070 1 NC_008702.1 Putative membrane protein, 32% identity to TrEMBL;Q7WHN2. Signal Peptide present.; Specificity unclear; hypothetical protein 996070 4609009 azo0929 Azoarcus sp. BH72 hypothetical protein YP_932433.1 995426 D 62928 CDS YP_932434.1 119897221 4606634 996074..997216 1 NC_008702.1 PQQ enzyme repeat protein, 40% identical(60% similarity)to TrEMBL;Q82XU7. Signal Peptide present. Has 6 copies SMART;SM00564, PQQ, beta-propeller repeat;IPR002372; Beta-propeller repeat occurrs in enzymes with pyrrolo-quinoline quinone (PQQ) as cofactor, in Ire1p-like Ser/Thr kinases, and in prokaryotic dehydrogenases.; Specificity unclear; hypothetical protein 997216 4606634 azo0930 Azoarcus sp. BH72 hypothetical protein YP_932434.1 996074 D 62928 CDS YP_932435.1 119897222 4606635 997232..998560 1 NC_008702.1 EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains; GTP-binding protein EngA 998560 engA 4606635 engA Azoarcus sp. BH72 GTP-binding protein EngA YP_932435.1 997232 D 62928 CDS YP_932436.1 119897223 4608668 998667..998909 1 NC_008702.1 Stimulates the elongation of poly(A) tails; RNA-binding protein Hfq 998909 hfq 4608668 hfq Azoarcus sp. BH72 RNA-binding protein Hfq YP_932436.1 998667 D 62928 CDS YP_932437.1 119897224 4608996 998991..1000130 1 NC_008702.1 GTP-binding protein hflX. trembl:Q7NS94: 66% identity; 78% similarity. InterPro:IPR006073; GTP1_OBG. These proteins contain GTP-binding motifs and are GTP1OBG Pfam:MMR_HSR1:GTPase of unknown function thdF: tRNA modification GTPase TrmE No Signal peptide present (SignalP predicted) Absence of transmembrane helices (TMHMM predicted); Family membership; GTP-binding subunit of protease specific for phage lambda 1000130 hflX1 4608996 hflX1 Azoarcus sp. BH72 GTP-binding subunit of protease specific for phage lambda YP_932437.1 998991 D 62928 CDS YP_932438.1 119897225 4608994 1000148..1001389 1 NC_008702.1 Putative HflK protein. Homology to hflK of E. coli of 40% (sprot|HFLK_ECOLI). hflc and hflk govern the stability of phage lambda cii protein and have been proposed to encode or regulate a cii- specific protease. Pfam: SPFH domain/band 7 family no signal peptide 1 TMH; Family membership; putative Hflk protein 1001389 hflK 4608994 hflK Azoarcus sp. BH72 putative Hflk protein YP_932438.1 1000148 D 62928 CDS YP_932439.1 119897226 4608993 1001389..1002270 1 NC_008702.1 Conserved hypothetical protein. Homology to hflC of E. coli of 37% (sprot|HFLC_ECOLI). hflc and hflk govern the stability of phage lambda cii protein and have been proposed to encode or regulate a cii- specific protease. Pfam: SPFH domain/ Band 7 family signal peptide; Family membership; hypothetical protein 1002270 hflC 4608993 hflC Azoarcus sp. BH72 hypothetical protein YP_932439.1 1001389 D 62928 CDS YP_932440.1 119897227 4608992 1002278..1002463 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE1283 of Nitrosomonas europaea of 60% (trembl|Q82V27). No domains predicted. Signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 1002463 4608992 azo0936 Azoarcus sp. BH72 hypothetical protein YP_932440.1 1002278 D 62928 CDS YP_932441.1 119897228 4606636 1002471..1003625 1 NC_008702.1 Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine; ATP phosphoribosyltransferase regulatory subunit 1003625 hisZ 4606636 hisZ Azoarcus sp. BH72 ATP phosphoribosyltransferase regulatory subunit YP_932441.1 1002471 D 62928 CDS YP_932442.1 119897229 4609010 1003629..1004939 1 NC_008702.1 catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis; adenylosuccinate synthetase 1004939 purA 4609010 purA Azoarcus sp. BH72 adenylosuccinate synthetase YP_932442.1 1003629 D 62928 CDS YP_932443.1 119897230 4610004 1005674..1008655 1 NC_008702.1 Ribonuclease R (EC 3.1.-.-) (RNase R) (VacB protein homolog). 3-exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A) poly(U) and ribosomal RNAs. Similar to SWISSPROT: sprot|RNR_ECOLI (38% Escherichia coli, ribonuclease R; RNase R / VacB protein) InterPro: IPR004476 3_prime_RNase. IPR003029 RNA binding S1. Pfam: PF00773 RNB-like protein. TIGRFAM: TIGR00358 VacB and RNase II family 3'-5' exoribonucleases.; High confidence in function and specificity; exoribonuclease II 1008655 vacB 4610004 vacB Azoarcus sp. BH72 exoribonuclease II YP_932443.1 1005674 D 62928 CDS YP_932444.1 119897231 4610087 1008648..1009412 1 NC_008702.1 Specificity unclear; tRNA/rRNA methyltransferase 1009412 4610087 azo0940 Azoarcus sp. BH72 tRNA/rRNA methyltransferase YP_932444.1 1008648 D 62928 CDS YP_932445.1 119897232 4606637 1009579..1010880 1 NC_008702.1 Probable nitrate regulatory protein,; High confidence in function and specificity; nitrate regulatory protein 1010880 4606637 azo0941 Azoarcus sp. BH72 nitrate regulatory protein YP_932445.1 1009579 D 62928 CDS YP_932446.1 119897233 4606638 1011215..1012474 1 NC_008702.1 Part of the ABC transporter complex NasFED involved in nitrate import. 59% Similar to the putative periplasmic-binding protein NasF in Klebsiella oxytoca. TREMBL:Q48466 InterPro:IPR010067; SsuA_fam.IPR006311; Tat. TIGRFAMs:TIGR01728; SsuA_fam; 1.TIGR01409; TAT_signal_seq; 1. Signal peptide present.; High confidence in function and specificity; nitrate transport system periplasmic-binding protein 1012474 nasF 4606638 nasF Azoarcus sp. BH72 nitrate transport system periplasmic-binding protein YP_932446.1 1011215 D 62928 CDS YP_932447.1 119897234 4609345 1012491..1013423 1 NC_008702.1 Nitrate transport permease nasE. 41% Similar to the nitrate permease, nrtB in Synechococcus sp.Part of the ABC transporter complex nrtBCD involved in nitrate import.Probably responsible for the translocation of the substrate across the membrane. SWISSPROT:NRTB_SYNP7.P38044. InterPro:IPR000515; BPD_transp.IPR005889; NtrB. Pfam:PF00528; BPD_transp; 1. TIGRFAMs:TIGR01183; ntrB; 1. TMHelix: 4.; High confidence in function and specificity; putative nitrate transport system permease 1013423 nasE 4609345 nasE Azoarcus sp. BH72 putative nitrate transport system permease YP_932447.1 1012491 D 62928 CDS YP_932448.1 119897235 4609344 1013436..1014230 1 NC_008702.1 Part of the ABC transporter complex nasFED involved in nitrate import. 64% Similar to the nitrate transport membrane protein, nasD in Klebsella oxytoca. Probably responsible for energy coupling to the transport system. SWISSPROT:NASD_KLEOX.P39459. InterPro:IPR003593; AAA_ATPase.IPR003439; ABC_transporter.IPR005890; NtrCD. Pfam:PF00005; ABC_tran; 1. TIGRFAMs:TIGR01184; ntrCD; 1.; High confidence in function and specificity; putative nitrate transport system ATP-binding protein 1014230 nasD 4609344 nasD Azoarcus sp. BH72 putative nitrate transport system ATP-binding protein YP_932448.1 1013436 D 62928 CDS YP_932449.1 119897236 4609343 1014601..1015365 1 NC_008702.1 Uroporphyrin-III C-methyltransferase (EC 2.1.1.107) (Urogen III methylase) (SUMT) (Uroporphyrinogen III methylase) (UROM). CATALYZES BOTH METHYLATIONS AT C-2 AND C-7 OF UROGEN III LEADING TO PRECORRIN-1 AND PRECORRIN-2; THEIR OXIDATIVE ESTERIFICATION GIVES RESPECTIVELY FACTOR I OCTAMETHYL ESTER AND SIROHYDROCHLORIN (BY SIMILARITY). dph5: diphthine synthase; High confidence in function and specificity; uroporphyrin-III C-methyltransferase 1015365 cobA1 4609343 cobA1 Azoarcus sp. BH72 uroporphyrin-III C-methyltransferase YP_932449.1 1014601 D 62928 CDS YP_932450.1 119897237 4608497 1015380..1016627 1 NC_008702.1 Putative nitrate reducatse small subunit. Homology to nasC of K. oxytoca of 38% (trembl|Q48467). NasC probably mediates electron transfer from NADH to NasA, the nitrate reductase. InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327) Pfam: Pyridine nucleotide-disulfide oxidoreductase no signal peptide no TMHs; Family membership; putative nitrate reductase small subunit 1016627 nasC 4608497 nasC Azoarcus sp. BH72 putative nitrate reductase small subunit YP_932450.1 1015380 D 62928 CDS YP_932451.1 119897238 4609342 1016664..1019438 1 NC_008702.1 Probable Nitrate reductase. Homology to nasA of K. oxytoca of 41% (sprot|NASA_KLEOX). Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants fungi and bacteria. InterPro: Prokaryotic molybdopterin oxidoreductases (IPR006655) Pfam: Molybdopterin oxidoreductase; Molydopterin dinucleotide binding domain no signal peptide no TMH Helixturnhelix motif; High confidence in function and specificity; nitrate reductase 1019438 nasA 4609342 nasA Azoarcus sp. BH72 nitrate reductase YP_932451.1 1016664 D 62928 CDS YP_932452.1 119897239 4609341 1019477..1020664 1 NC_008702.1 Putative methyl-accepting chemotaxis protein,; Specificity unclear; putative methyl-accepting chemotaxis protein 1020664 4609341 azo0948 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_932452.1 1019477 D 62928 CDS YP_932453.1 119897240 4606639 1020736..1021629 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002329 of Dechloromonas aromatica of 40% (gi|53730441|ref|ZP_00348795.1|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 1021629 4606639 azo0949 Azoarcus sp. BH72 hypothetical protein YP_932453.1 1020736 D 62928 CDS YP_932454.1 119897241 4606640 1021632..1023464 1 NC_008702.1 GGEF/EAL/PAS/PAC-domain containing protein; GGEF/EAL/PAS/PAC-domain-containing protein 1023464 4606640 azo0950 Azoarcus sp. BH72 GGEF/EAL/PAS/PAC-domain-containing protein YP_932454.1 1021632 D 62928 CDS YP_932455.1 119897242 4606641 1023516..1023875 1 NC_008702.1 50%Cupin.IPR007113; Cupin_sup. Pfam:PF00190; Cupin; 1. 28%; phosphomannose protein 1023875 4606641 azo0951 Azoarcus sp. BH72 phosphomannose protein YP_932455.1 1023516 D 62928 CDS YP_932456.1 119897243 4606642 1024028..1024933 1 NC_008702.1 Conserved hypothetical protein, 38% identity (50% similarity) to TrEMBL;Q7WXG4 Signal Peptide Present.TMH not Present. Has PF04471:Restriction endonuclease;(IPR007560:Mrr_cat):Prokaryotic family found in type II restriction enzymes containing the hallmark (D/E)-(D/E)XK active site. Presence of catalytic residues implicates this region in the enzymatic cleavage of DNA. PF01396:Topoisomerase DNA binding C4 zinc finger;(IPR000380 DNA_tpisomrase):Prokaryotic topoisomerase I , otherwise known as relaxing enzyme,untwisting enzyme or swivelase, catalyses the ATP-independent breakage of single- stranded DNA, followed by passage and rejoining of another single-stranded DNA region [4]. This reaction brings about the conversion of one topological isomer of DNA into another: e.g.,relaxation of superhelical turns; interconversion of simple and knotted rings of single-stranded DNA; and intertwisting of single-stranded rings of complementary sequences.; Family membership; hypothetical protein 1024933 4606642 azo0952 Azoarcus sp. BH72 hypothetical protein YP_932456.1 1024028 D 62928 CDS YP_932457.1 119897244 4606643 complement(1024961..1025785) 1 NC_008702.1 Putative oxygen-insensitive NADPH nitroreductase. Homology to snrA of S. typhimurium of 37% (sprot|NFSA_SALTY) REDUCTION OF NITROAROMATIC COMPOUNDS USING NADH. REDUCES NITROFURAZONE BY A PING-PONG BI-BI MECHANISM POSSIBLY TO GENERATE A TWO-ELECTRON TRANSFER PRODUCT. MAJOR COMPONENT OF THE OXYGEN- INSENSITIVE NITROREDUCTASE ACTIVITY IN E.COLI. Pfam: Nitroreductase family no signal peptide no TMHs; Family membership; putative oxygen-insentive NADPH nitroreductase 1025785 nfsA 4606643 nfsA Azoarcus sp. BH72 putative oxygen-insentive NADPH nitroreductase YP_932457.1 1024961 R 62928 CDS YP_932458.1 119897245 4609354 complement(1025891..1026424) 1 NC_008702.1 Probable flavodoxin. Homology to isiB of Synechococcus sp. of 45% (pir|B47673) Flavodoxins are electron-transfer proteins that function in various electron transport systems. Flavodoxins bind one FMN molecule, which serves as a redox-active prosthetic group and are functionally interchangeable with ferredoxins. Pfam: Flavodoxin no signal peptide no TMHs; High confidence in function and specificity; flavodoxin 1026424 isiB 4609354 isiB Azoarcus sp. BH72 flavodoxin YP_932458.1 1025891 R 62928 CDS YP_932459.1 119897246 4609092 complement(1026770..1027678) 1 NC_008702.1 Putative GufA protein. 31% Zn_transpt_Zip.InterPro: ZIP Zinc transporter Pfam: PF02535; Zip; 1. TMHelix: 9. Signal peptide present.; Specificity unclear; putative cation transporter protein 1027678 gufA 4609092 gufA Azoarcus sp. BH72 putative cation transporter protein YP_932459.1 1026770 R 62928 CDS YP_932460.1 119897247 4608964 complement(1027781..1029013) 1 NC_008702.1 catalyzes the transfer of a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge; cyclopropane-fatty-acyl-phospholipid synthase 1029013 cfa1 4608964 cfa1 Azoarcus sp. BH72 cyclopropane-fatty-acyl-phospholipid synthase YP_932460.1 1027781 R 62928 CDS YP_932461.1 119897248 4608462 1029315..1031303 1 NC_008702.1 DNA polymerase III subunit tau (EC 2.7.7.7) [Contains: DNA polymerase III subunit gamma]. The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA. InterPro: AAA-protein (ATPases associated with various cellular activities); High confidence in function and specificity; DNA polymerase III subunit tau 1031303 dnaX 4608462 dnaX Azoarcus sp. BH72 DNA polymerase III subunit tau YP_932461.1 1029315 D 62928 CDS YP_932462.1 119897249 4608640 1031349..1031678 1 NC_008702.1 conserved hypothetical protein Pfam: Uncharacterized BCR, YbaB family; hypothetical protein 1031678 4608640 azo0958 Azoarcus sp. BH72 hypothetical protein YP_932462.1 1031349 D 62928 CDS YP_932463.1 119897250 4606644 1031759..1032358 1 NC_008702.1 involved in a recombinational process of DNA repair, independent of the recBC complex; recombination protein RecR 1032358 recR 4606644 recR Azoarcus sp. BH72 recombination protein RecR YP_932463.1 1031759 D 62928 CDS YP_932464.1 119897251 4606645 1032540..1033133 1 NC_008702.1 Probable ubiquinol-cytochrome c reductase iron-sulfur protein (Rieske iron-sulfur protein) (RISP). Homology to petA of R. gelantinosus of 63% (trembl|Q93SY7). Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Pfam: Rieske [2Fe-2S] domain singal peptide no TMHs; High confidence in function and specificity; ubiquinol-cytochrome c reductase iron-sulfur protein 1033133 petA1 4606645 petA1 Azoarcus sp. BH72 ubiquinol-cytochrome c reductase iron-sulfur protein YP_932464.1 1032540 D 62928 CDS YP_932465.1 119897252 4609524 1033137..1034405 1 NC_008702.1 Ubiquinol-cytochrome c reductase cytochrome b protein. Homology to petB of R. gelatinosus of 72% (trembl|Q93SY6). Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity). Pfam: Cytochrome b (N-terminal)/b6/petB; cytochrome b (C-terminal)/b6/petD no signal peptide 10 TMHs; High confidence in function and specificity; ubiquinol-cytochrome c reductase cytochrome b protein 1034405 petB 4609524 petB Azoarcus sp. BH72 ubiquinol-cytochrome c reductase cytochrome b protein YP_932465.1 1033137 D 62928 CDS YP_932466.1 119897253 4609526 1034402..1035157 1 NC_008702.1 Conserved hypothetical ubiquinol-cytochrome c reductase cytochrome c1 protein. Homology to petC of R. solanacearum of 52% (trembl|Q8XVA4). Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. c1 functions as an electron donor to cytochrome c. InterPro: Cytochrome c1 (IPR002326) Pfam: Cytochrome_c1 signal peptide 1 TMH; Conserved hypothetical protein; putative ubiquinol-cytochrome c reductase cytochrome c1 protein 1035157 petC 4609526 petC Azoarcus sp. BH72 putative ubiquinol-cytochrome c reductase cytochrome c1 protein YP_932466.1 1034402 D 62928 CDS YP_932467.1 119897254 4609527 1035265..1035861 1 NC_008702.1 Probable Stringent starvation protein A. Homology to sspA of E. coli of 43% (sprot|SSPA_ECOLI). FORMS AN EQUIMOLAR COMPLEX WITH THE RNA POLYMERASE HOLOENZYME (RNAP) BUT NOT WITH THE CORE ENZYME. IT IS SYNTHESIZED PREDOMINANTLY WHEN CELLS ARE EXPOSED TO AMINO ACID STARVATION AT WHICH TIME IT ACCOUNTS FOR OVER 50% OF THE TOTAL PROTEIN SYNTHESIZED. Pfam: Glutatione S-transferase,N-terminale domaine no TMHs; Family membership; stringent starvation protein A 1035861 sspA2 4609527 sspA2 Azoarcus sp. BH72 stringent starvation protein A YP_932467.1 1035265 D 62928 CDS YP_932468.1 119897255 4609896 1035915..1036355 1 NC_008702.1 Putative stringent starvation protein B. Homology to sspB of E. coli of 38% (sprot|SSPB_ECOLI) Seems to act in concert with sspA in the regulation of several proteins during exponential and stationary-phase growth. The exact function of sspB is not yet known. Pfam: Stringent starvation protein B no signal peptide no TMHs; ClpXP protease specificity-enhancing factor 1036355 sspB 4609896 sspB Azoarcus sp. BH72 ClpXP protease specificity-enhancing factor YP_932468.1 1035915 D 62928 CDS YP_932469.1 119897256 4609897 complement(1036339..1037562) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Family membership; glycosyltransferase 1037562 4609897 azo0965 Azoarcus sp. BH72 glycosyltransferase YP_932469.1 1036339 R 62928 CDS YP_932470.1 119897257 4606646 1037729..1038217 1 NC_008702.1 Hemerythrin-like protein, 49% Identity to SProt;Q8Y1B3. Has PF01814, Hemerythrin HHE cation binding domain; IPR002063, Hemerythrin; Iteration of the HHE family found it to be related to Hemerythrin. It also demonstrated that what has been described as a single domain in fact consists of two cation binding domains. Members of this family occur all across nature and are involved in a variety of processes. For instance, in Nereis diversicolor P80255 binds Cadmium so as to protect the organism from toxicity . However Hemerythrin is classically described as Oxygen-binding through two attached Fe2+ ions. And the bacterial Q7WX96 is a regulator of response to NO, which suggests yet another set-up for its metal ligands. In Staphylococcus aureus P72360 has been noted to be important when the organism switches to living in environments with low oxygen concentrations; perhaps this protein acts as an oxygen store or scavenger.; cation-binding hemerythrin HHE family protein 1038217 4606646 azo0966 Azoarcus sp. BH72 cation-binding hemerythrin HHE family protein YP_932470.1 1037729 D 62928 CDS YP_932471.1 119897258 4606647 complement(1038235..1039485) 1 NC_008702.1 The L-alanine catabolic pathway proceeds in two steps: racemization of the L-isomer to D-alanine by alanine racemase and oxidative deamination of D-alanine to pyruvate and ammonia by D- amino acid dehydrogenase. Similar to trembl|Q7NRT8 (52%) and to sprot|DADA_ECOLI (40%). Pfam (PF01266): D-amino acid oxidase; Specificity unclear; D-amino acid dehydrogenase small subunit 1039485 dadA1 4606647 dadA1 Azoarcus sp. BH72 D-amino acid dehydrogenase small subunit YP_932471.1 1038235 R 62928 CDS YP_932472.1 119897259 4608578 1039663..1040664 1 NC_008702.1 catalyzes the formation of D-fructose 6-phosphate from fructose-1,6-bisphosphate; fructose-1,6-bisphosphatase 1040664 cbbF 4608578 cbbF Azoarcus sp. BH72 fructose-1,6-bisphosphatase YP_932472.1 1039663 D 62928 CDS YP_932473.1 119897260 4608421 1040676..1042403 1 NC_008702.1 conserved hypothetical outer membrane protein. Homology to ne0930 of N. europaea of 44% (trembl|Q82VX0) Pfam: Bacterial surface protein signal peptide no TMHs; Family membership; outer membrane protein 1042403 4608421 azo0969 Azoarcus sp. BH72 outer membrane protein YP_932473.1 1040676 D 62928 CDS YP_932474.1 119897261 4606648 1042491..1043462 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bb3356 of B. bronchiseptica of 83% (trembl|Q7WH54). Pfam: DUF534 This is a family of putative secreted proteins of unknown function. signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 1043462 4606648 azo0970 Azoarcus sp. BH72 hypothetical protein YP_932474.1 1042491 D 62928 CDS YP_932475.1 119897262 4606649 1043538..1044428 1 NC_008702.1 Conserved hypothetical ABC transporter permease. PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM. PROBABLY RESPONSIBLE FOR THE TRANSLOCATION OF THE SUBSTRATE ACROSS THE MEMBRANE. InterPro: Binding-system dependent bacterial transporters (araH livH/limM families) (IPR001851) Pfam: Branched-chain amino acid transport system/permease component no signal peptide 8 TMHs 2A78: Carboxylate/Amino Acid/Amine Tran; Specificity unclear; ABC transporter permease 1044428 4606649 azo0971 Azoarcus sp. BH72 ABC transporter permease YP_932475.1 1043538 D 62928 CDS YP_932476.1 119897263 4606650 1044430..1045224 1 NC_008702.1 Thiamine transport ATP-binding protein thiQ. PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM TBPA-THIPQ FOR THIAMINE AND TPP. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. TREMBL:Q7WH56: 84% identity, 92% similarity Description:putative abc-transporter atp-binding component InterPro: IPR003593; AAA_ATPase. IPR003439; ABC_transporter. Pfam:PF00005; ABC_tran; ProDom PD000006; ABC_transporter; 1. SMART SM00382; AAA recf: DNA replication and repair protein NO transmembrane helices; Function unclear; ABC transporter ATP-binding protein 1045224 4606650 azo0972 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932476.1 1044430 D 62928 CDS YP_932477.1 119897264 4606651 1045436..1045726 1 NC_008702.1 Chaperonin GroES. Homolgy to groES of B. japonicum of 61% (sprot|CH11_BRAJA) Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. InterPro: Chaperonins cpn10 (10 Kd subunit)(IPR001476) Pfam: Chaperonin 10 kd subunit no signal peptide no TMH; High confidence in function and specificity; chaperonin GroES 1045726 groES1 4606651 groES1 Azoarcus sp. BH72 chaperonin GroES YP_932477.1 1045436 D 62928 CDS YP_932478.1 119897265 4608940 1045775..1047424 1 NC_008702.1 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; chaperonin GroEL 1047424 groEL 4608940 groEL Azoarcus sp. BH72 chaperonin GroEL YP_932478.1 1045775 D 62928 CDS YP_932479.1 119897266 4608937 1047710..1048396 1 NC_008702.1 Probable two-component response regulatory protein,Response_reg. IPR001867; Trans_reg_C. Pfam: PF00072; response_reg. PF00486; trans_reg_C. SMART: SM00448; REC.; Specificity unclear; two-component response regulatory protein 1048396 basR 4608937 basR Azoarcus sp. BH72 two-component response regulatory protein YP_932479.1 1047710 D 62928 CDS YP_932480.1 119897267 4608377 1048371..1049858 1 NC_008702.1 Putative two-component system histidine kinase,; Specificity unclear; putative two-component system histidine kinase 1049858 basS 4608377 basS Azoarcus sp. BH72 putative two-component system histidine kinase YP_932480.1 1048371 D 62928 CDS YP_932481.1 119897268 4608378 1050037..1050345 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to XCC4058 of Xanthomonas campestris of 55% (trembl|Q8P3L3) Has PF04341, Protein of unknown function,DUF485; IPR007436;This family includes several putative integral membrane proteins. no signal peptide 1 TMH; Conserved hypothetical protein; hypothetical protein 1050345 4608378 azo0977 Azoarcus sp. BH72 hypothetical protein YP_932481.1 1050037 D 62928 CDS YP_932482.1 119897269 4606652 1050342..1052060 1 NC_008702.1 member of the sodium:solute symporter family; cotranscribed with the acs gene which encodes acetyl coenzyme A synthase; mutations affect acetate uptake; acetate permease 1052060 actP 4606652 actP Azoarcus sp. BH72 acetate permease YP_932482.1 1050342 D 62928 CDS YP_932483.1 119897270 4606653 complement(1052174..1052845) 1 NC_008702.1 Probable transcriptional regulatory protein,Response_reg. IPR001867; Trans_reg_C. Pfam: PF00072; response_reg. PF00486; trans_reg_C. SMART: SM00448; REC. Transcriptional regulatory protein tctD. TRANSCRIPTIONAL ACTIVATOR OF THE TCTI TRICARBOXYLATE TRANSPORT SYSTEM OPERON.; Specificity unclear; transcriptional regulator 1052845 tctD 4606653 tctD Azoarcus sp. BH72 transcriptional regulator YP_932483.1 1052174 R 62928 CDS YP_932484.1 119897271 4610012 1053183..1054436 1 NC_008702.1 catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins; glutamyl-tRNA reductase 1054436 hemA 4610012 hemA Azoarcus sp. BH72 glutamyl-tRNA reductase YP_932484.1 1053183 D 62928 CDS YP_932485.1 119897272 4606654 1054469..1055554 1 NC_008702.1 recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2; peptide chain release factor 1 1055554 prfA 4606654 prfA Azoarcus sp. BH72 peptide chain release factor 1 YP_932485.1 1054469 D 62928 CDS YP_932486.1 119897273 4609623 1055551..1056387 1 NC_008702.1 Protein methyltransferase hemK (EC 2.1.1.-) (Protein-glutamine N- methyltransferase hemK) (Protein-(glutamine-N5) MTase hemK) (M.StyLTHemKP). Methylates the translation termination release factor RF1 on Gln-235 and RF2 on Gln-252.; Family membership; HemK protein 1056387 hemK 4609623 hemK Azoarcus sp. BH72 HemK protein YP_932486.1 1055551 D 62928 CDS YP_932487.1 119897274 4608986 1056474..1056797 1 NC_008702.1 Conserved hypothetical protein. Homology to ne1911 of N. europaea of 67%. InterPro: Glutaredoxin-related protein (IPR004480) TIGR00365: glutaredoxin-related protein no signal peptide no TMHs; hypothetical protein 1056797 4608986 azo0983 Azoarcus sp. BH72 hypothetical protein YP_932487.1 1056474 D 62928 CDS YP_932488.1 119897275 4606655 complement(1056828..1057925) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NE1634 of Nitrosomonas europaea of 34% (trembl|Q82U71(SRS)) No domains predicted. No TMHs Signal peptide present.; Conserved hypothetical protein; hypothetical protein 1057925 4606655 azo0984 Azoarcus sp. BH72 hypothetical protein YP_932488.1 1056828 R 62928 CDS YP_932489.1 119897276 4606656 complement(1057916..1058776) 1 NC_008702.1 Conserved hypothetical protein, 35% identity (56% similarity) to TrEMBL;Q82U70. Signal peptide Present. No TMH reported Present.; hypothetical protein 1058776 4606656 azo0985 Azoarcus sp. BH72 hypothetical protein YP_932489.1 1057916 R 62928 CDS YP_932490.1 119897277 4606657 complement(1058789..1059091) 1 NC_008702.1 Hypothetical Protein. No Good functional or similar proteins matching in the database. No domains, repeats,motifs or features present.; hypothetical protein 1059091 4606657 azo0986 Azoarcus sp. BH72 hypothetical protein YP_932490.1 1058789 R 62928 CDS YP_932491.1 119897278 4606658 complement(1059121..1059837) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 1059837 4606658 azo0987 Azoarcus sp. BH72 hypothetical protein YP_932491.1 1059121 R 62928 CDS YP_932492.1 119897279 4606659 complement(1060017..1061303) 1 NC_008702.1 Putative serine/threonine protein kinase,; Family membership; putative serine/threonine protein kinase 1061303 4606659 azo0988 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_932492.1 1060017 R 62928 CDS YP_932493.1 119897280 4606660 complement(1061310..1062752) 1 NC_008702.1 catalyzes the formation of arginine from (N-L-arginino)succinate; argininosuccinate lyase 1062752 argH 4606660 argH Azoarcus sp. BH72 argininosuccinate lyase YP_932493.1 1061310 R 62928 CDS YP_932494.1 119897281 4608337 1062841..1063866 1 NC_008702.1 Putative alginate biosynthesis protein AlgZ/FimS,; High confidence in function and specificity; putative alginate biosynthesis protein AlgZ/FimS 1063866 algZ 4608337 algZ Azoarcus sp. BH72 putative alginate biosynthesis protein AlgZ/FimS YP_932494.1 1062841 D 62928 CDS YP_932495.1 119897282 4608301 1063863..1064624 1 NC_008702.1 Probable alginate biosynthesis regulatory protein AlgR,; High confidence in function and specificity; alginate biosynthesis regulatory protein AlgR 1064624 algR 4608301 algR Azoarcus sp. BH72 alginate biosynthesis regulatory protein AlgR YP_932495.1 1063863 D 62928 CDS YP_932496.1 119897283 4608298 complement(1064654..1067407) 1 NC_008702.1 catalyzes the formation of oxaloacetate from phosphoenolpyruvate; phosphoenolpyruvate carboxylase 1067407 ppc 4608298 ppc Azoarcus sp. BH72 phosphoenolpyruvate carboxylase YP_932496.1 1064654 R 62928 CDS YP_932497.1 119897284 4609608 1067713..1068663 1 NC_008702.1 transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis; porphobilinogen deaminase 1068663 hemC 4609608 hemC Azoarcus sp. BH72 porphobilinogen deaminase YP_932497.1 1067713 D 62928 CDS YP_932498.1 119897285 4608981 1068679..1069476 1 NC_008702.1 Uroporphyrinogen-III synthase (Uroporphyrinogen-III cosynthetase) (Hydroxymethylbilane hydrolyase [cyclizing]). catalytic activity: hydroxymethylbilane = uroporphyrinogen-iii + h(2)o. pathway:porphyrin biosynthesis; fourth step. ribH: riboflavin synthase beta subunit; Specificity unclear; HemD protein 1069476 hemD 4608981 hemD Azoarcus sp. BH72 HemD protein YP_932498.1 1068679 D 62928 CDS YP_932499.1 119897286 4608982 1069486..1070682 1 NC_008702.1 Putative uroporphyrin-III C-methyltransferase. catalytic activity: 2 s-adenosyl-l-methionine + uroporphyrin iii = 2 s-adenosyl-l-homocysteine + sirohydrochlorin. pathway: siroheme and cobalamin biosynthesis.; Specificity unclear; uroporphyrin-III C-methyltransferase 1070682 hemX 4608982 hemX Azoarcus sp. BH72 uroporphyrin-III C-methyltransferase YP_932499.1 1069486 D 62928 CDS YP_932500.1 119897287 4608989 1070695..1071894 1 NC_008702.1 HemY protein. involved in a late step of protoheme ix synthesis.; High confidence in function and specificity; HemY protein 1071894 hemY 4608989 hemY Azoarcus sp. BH72 HemY protein YP_932500.1 1070695 D 62928 CDS YP_932501.1 119897288 4608990 1072050..1072769 1 NC_008702.1 Glc operon transcriptional activator,; High confidence in function and specificity; glc operon transcriptional activator 1072769 glcC 4608990 glcC Azoarcus sp. BH72 glc operon transcriptional activator YP_932501.1 1072050 D 62928 CDS YP_932502.1 119897289 4608879 1072863..1074362 1 NC_008702.1 Probable glycolate oxidase subunit glcD. Homology to glcD of E. coli of 64% (sprot|GLCD_ECOLI) InterPro: FAD linked oxidase C-terminal (IPR004113); FAD linked oxidase N-terminal (IPR006093) Pfam: FAD binding domain; FAD linked oxidase, C-terminal domain Tigrfam: glcD: glycolate oxidase subunit GlcD no signal peptide no TMHs; High confidence in function and specificity; glycolate oxidase subunit GlcD 1074362 glcD1 4608879 glcD1 Azoarcus sp. BH72 glycolate oxidase subunit GlcD YP_932502.1 1072863 D 62928 CDS YP_932503.1 119897290 4608880 1074367..1074513 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal pepitde. 1 TMH; hypothetical protein 1074513 4608880 azo0999 Azoarcus sp. BH72 hypothetical protein YP_932503.1 1074367 D 62928 CDS YP_932504.1 119897291 4606661 1074510..1075571 1 NC_008702.1 Probable glycolate oxidase subunit GlcE. Homology to glcE of E. coli of 47% (sprot|GLCE_ECOLI) InterPro: FAD linked oxidase N-terminal (IPR006093) Pfam: FAD binding domain Tigrfam: glcD: glycolate oxidase subunit GlcD no singla peptide no TMHs; High confidence in function and specificity; glycolate oxidase FAD binding subunit 1075571 glcE 4606661 glcE Azoarcus sp. BH72 glycolate oxidase FAD binding subunit YP_932504.1 1074510 D 62928 CDS YP_932505.1 119897292 4608882 1075575..1076819 1 NC_008702.1 Probable glycolate oxidase iron-sulfur subunit. Homology to glcF of E. coli of 56% (sprot|GLCF_ECOLI) Pfam: Domain of unknown function (DUF224) (192-256 aa; 329-391 aa) no signal peptide no TMHs; High confidence in function and specificity; glycolate oxidase iron-sulfur subunit 1076819 glcF 4608882 glcF Azoarcus sp. BH72 glycolate oxidase iron-sulfur subunit YP_932505.1 1075575 D 62928 CDS YP_932506.1 119897293 4608883 1076945..1077721 1 NC_008702.1 Putative phosphoprotein phosphatase,; Conserved hypothetical protein; putative phosphoprotein phosphatase 1077721 4608883 azo1002 Azoarcus sp. BH72 putative phosphoprotein phosphatase YP_932506.1 1076945 D 62928 CDS YP_932507.1 119897294 4606662 1077900..1079021 1 NC_008702.1 Probable NAD(P) transhydrogenase, subunit alpha part 1. Homology to pntAA of R. rubrum of 44% (sprot|PNAA_RHORU). The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. Tirfam: pntA: NAD(P) transhydrogenase alpha subunit Pfam: Alanine dehydrogenase/pyridine nucleotide dehydrogenase no signal peptide no TMHs; High confidence in function and specificity; NAD(P) transhydrogenase subunit alpha part 1 1079021 pntAA 4606662 pntAA Azoarcus sp. BH72 NAD(P) transhydrogenase subunit alpha part 1 YP_932507.1 1077900 D 62928 CDS YP_932508.1 119897295 4609590 1079035..1079343 1 NC_008702.1 Conserved hypothetical NAD(P) transhydrogenase,subunit alpha part 2. Homology to pntA of X. axonopodis of 74% (trembl|Q8PNW6). The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. 3 TMHs no signal peptide; High confidence in function and specificity; NAD(P) transhydrogenase subunit alpha part 2 1079343 pntAB 4609590 pntAB Azoarcus sp. BH72 NAD(P) transhydrogenase subunit alpha part 2 YP_932508.1 1079035 D 62928 CDS YP_932509.1 119897296 4609591 1079340..1080719 1 NC_008702.1 Probable NAD(P) transhydrogenase, subunit beta. Homology to pntB of R. rubrum of 46% (sprot|PNTB_RHORU) The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. Pfam: NAD(P) transhydrogenase beta subunit signal peptide 7 TMHs; High confidence in function and specificity; NAD(P) transhydrogenase subunit beta 1080719 pntB 4609591 pntB Azoarcus sp. BH72 NAD(P) transhydrogenase subunit beta YP_932509.1 1079340 D 62928 CDS YP_932510.1 119897297 4609592 1080834..1081184 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 1081184 4609592 azo1006 Azoarcus sp. BH72 hypothetical protein YP_932510.1 1080834 D 62928 CDS YP_932511.1 119897298 4606663 complement(1081178..1083613) 1 NC_008702.1 Hypothetical signaling protein; signaling protein 1083613 4606663 azo1007 Azoarcus sp. BH72 signaling protein YP_932511.1 1081178 R 62928 CDS YP_932512.1 119897299 4606664 complement(1083711..1085195) 1 NC_008702.1 Putative ATP-dependent RNA helicase rhlE.; Family membership; putative ATP-dependent RNA helicase 1085195 rhlE2 4606664 rhlE2 Azoarcus sp. BH72 putative ATP-dependent RNA helicase YP_932512.1 1083711 R 62928 CDS YP_932513.2 228906474 4609717 complement(1085393..1086454) 1 NC_008702.1 Conserved hypothetical protein. Homology to xcc0816 of X. campestris of 49% (trembl|Q8PCB8) Pfam: FtsJ-like methyltransferase no signal peptide no TMHs; putative RNA 2'-O-ribose methyltransferase 1086454 4609717 azo1009 Azoarcus sp. BH72 putative RNA 2'-O-ribose methyltransferase YP_932513.2 1085393 R 62928 CDS YP_932514.1 119897301 4606665 1086606..1087658 1 NC_008702.1 Probable oxidoreductase Tas. Homology to tas of E. coli of 51% (sprot|TAS_ECOLI). InterPro: Aldo/keto reductase family (IPR001395) Pfam: Aldo/keto reductase family no signal peptide no TMHs; Function unclear; oxidoreductase Tas 1087658 tas 4606665 tas Azoarcus sp. BH72 oxidoreductase Tas YP_932514.1 1086606 D 62928 CDS YP_932515.1 119897302 4609917 complement(1087666..1088040) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Rgel02001790 of Rubrivivax gelatinosus of 45% (gi|47573892|ref|ZP_00243929.1|(NBCI ENTREZ)) . No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 1088040 4609917 azo1011 Azoarcus sp. BH72 hypothetical protein YP_932515.1 1087666 R 62928 CDS YP_932516.1 119897303 4606666 1088233..1089531 1 NC_008702.1 Glycerol-3-phosphate-binding periplasmic protein precursor. SN-glycerol-3-phosphate and glycerophosphoryl diester-binding protein interacts with the binding protein-dependent transport system ugpACE. 30% SBP_bac_1.IPR006061; SBP_dom1. Pfam:PF01547; SBP_bac_1; 1. Signal peptide:present.; High confidence in function and specificity; putative glycerol-3-phosphate-binding periplasmic protein 1089531 ugpB 4606666 ugpB Azoarcus sp. BH72 putative glycerol-3-phosphate-binding periplasmic protein YP_932516.1 1088233 D 62928 CDS YP_932517.1 119897304 4610065 1089536..1090285 1 NC_008702.1 hydrolyzes diesters during transport at the inner face of the cytoplasmic membrane to glycerol-3-phosphate and alcohol; induced when cells are starved for inorganic phosphate; glycerophosphodiester phosphodiesterase 1090285 ugpQ 4610065 ugpQ Azoarcus sp. BH72 glycerophosphodiester phosphodiesterase YP_932517.1 1089536 D 62928 CDS YP_932518.1 119897305 4610066 1090402..1091427 1 NC_008702.1 Polyprenyl synthetase,82% identity to TrEMBL;Q5P1Y1. Has Pfam;PF00348, Polyprenyl synthetase.(IPR000092,Polyprenyl_synt)A variety of isoprenoid compounds are synthesized by various organisms. For example in eukaryotes the isoprenoid biosynthetic pathway is responsible for the synthesis of a variety of end products including cholesterol, dolichol, ubiquinone or coenzyme Q. In bacteria this pathway leads to the synthesis of isopentenyl tRNA, isoprenoid quinones, and sugar carrier lipids. Among the enzymes that participate in that pathway, are a number of polyprenyl synthetase enzymes which catalyze a 1'4-condensation between 5 carbon isoprene units. It has been shown that all the above enzymes share some regions of sequence similarity. Two of these regions are rich in aspartic-acid residues and could be involved in the catalytic mechanism and/or the binding of the substrates.; High confidence in function and specificity; octaprenyl-diphosphate synthase 1091427 ispB 4610066 ispB Azoarcus sp. BH72 octaprenyl-diphosphate synthase YP_932518.1 1090402 D 62928 CDS YP_932519.1 119897306 4610013 1091631..1092566 1 NC_008702.1 Probable transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 1092566 4610013 azo1015 Azoarcus sp. BH72 LysR family transcriptional regulator YP_932519.1 1091631 D 62928 CDS YP_932520.1 119897307 4606667 1092645..1093793 1 NC_008702.1 L-lactate dehydrogenase. Homology to lldA of N. meningitidis of 72% (trembl|Q51135). InterPro: FMN-dependent alpha-hydroxy acid dehydrogenases (IPR000262); Proteins binding FMN and related compounds core region (IPR003009 Pfam: FMN-dependent dehydrogenase no signal peptide no TMHs; High confidence in function and specificity; L-lactate dehydrogenase 1093793 lldA 4606667 lldA Azoarcus sp. BH72 L-lactate dehydrogenase YP_932520.1 1092645 D 62928 CDS YP_932521.1 119897308 4609164 complement(1093840..1094649) 1 NC_008702.1 probable competence lipoprotein comL precursor. Homology to comL of N. gonorrhoeae of 47% (sprot|COML_NEIGO(SRS)) REQUIRED FOR EFFICIENT TRANSFORMATION OF NEISSERIA MENINGITIDIS BY SPECIES-RELATED DNA (By similarity). InterPro: TPR repeat (IPR001440) no signal peptide no TMH ccoS: cytochrome oxidase maturation pro; High confidence in function and specificity; competence lipoprotein 1094649 comL 4609164 comL Azoarcus sp. BH72 competence lipoprotein YP_932521.1 1093840 R 62928 CDS YP_932522.1 119897309 4608512 1094663..1095637 1 NC_008702.1 Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil at positions 1911 1915 and 1917 in 23S ribosomal RNA.; Family membership; pseudouridylate synthase 1095637 rluD 4608512 rluD Azoarcus sp. BH72 pseudouridylate synthase YP_932522.1 1094663 D 62928 CDS YP_932523.1 119897310 4609734 1095700..1096395 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2191 of C.violaceum of 64% (tremble:Q7NVZ9). Has PF02578,Uncharacterised ACR, YfiH family COG1496. InterPro;IPR003730. No signal peptide. No TMHs. TIGR00726: conserved hypothetical protein; hypothetical protein 1096395 4609734 azo1019 Azoarcus sp. BH72 hypothetical protein YP_932523.1 1095700 D 62928 CDS YP_932524.1 119897311 4606668 complement(1096434..1096655) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS03993 of R.solanacearum of 48% (trembl|Q8XYX6(SRS)). No domains. No signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 1096655 4606668 azo1020 Azoarcus sp. BH72 hypothetical protein YP_932524.1 1096434 R 62928 CDS YP_932525.1 119897312 4606669 1096743..1098485 1 NC_008702.1 Entry name :- TREMBL:Q9ZB54 Prim. accession # Q9ZB54 Identities = 290/560 (51%) InterPro:- IPR000073; A/b_hydrolase. IPR010941; PhaC_N. Pfam:- PF00561; Abhydrolase_1; 1. PF07167; PhaC_N; 1. Prediction: Signal peptide Signal peptide probability: 0.999 Number of predicted TMHs: 0; Family membership; polyhydroxyalkanoate synthase 1098485 phaC 4606669 phaC Azoarcus sp. BH72 polyhydroxyalkanoate synthase YP_932525.1 1096743 D 62928 CDS YP_932526.1 119897313 4609535 1098596..1099336 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,SWISSPROT:P14697 (60% identity); TREMBL:Q8XYX3 (59% identity). Pfam (PF00106): Short chain dehydrogenase.; High confidence in function and specificity; acetoacetyl-CoA reductase 1099336 phbB1 4609535 phbB1 Azoarcus sp. BH72 acetoacetyl-CoA reductase YP_932526.1 1098596 D 62928 CDS YP_932527.1 119897314 4609539 1099528..1100268 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,SWISSPROT:P14697 (62% identity); TREMBL:Q8XYX3 (62% identiy). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase. SignalP reporting signal peptide.; High confidence in function and specificity; acetoacetyl-CoA reductase 1100268 phbB2 4609539 phbB2 Azoarcus sp. BH72 acetoacetyl-CoA reductase YP_932527.1 1099528 D 62928 CDS YP_932528.1 119897315 4609540 1100389..1101018 1 NC_008702.1 phbF protein, 57% identity to TrEMBL;Q7NYA7. Has 2 copies of PF05233, PHB accumulation regulatory domain;IPR007897, PHB_accumulat: The proteins this domain is found in are typically involved in regulating polymer accumulation in bacteria, particularly poly-beta-hydroxybutyrate (PHB); High confidence in function and specificity; hypothetical protein 1101018 phbF 4609540 phbF Azoarcus sp. BH72 hypothetical protein YP_932528.1 1100389 D 62928 CDS YP_932529.1 119897316 4609543 1101080..1102411 1 NC_008702.1 catalyzes the methylthiolation of an aspartic acid residue of the S12 protein of the 30S ribosomal subunit; ribosomal protein S12 methylthiotransferase 1102411 rimO 4609543 rimO Azoarcus sp. BH72 ribosomal protein S12 methylthiotransferase YP_932529.1 1101080 D 62928 CDS YP_932530.1 119897317 4606670 1102525..1103373 1 NC_008702.1 Hypothetical protein ychK. TREMBL:Q89IT4: 42% identity, 58% similarity InterPro: Uncharacterized protein family UPF0028 InterPro:IPR002641; Patatin. Pfam:PF01734; Patatin hypA: hydrogenase expression/formation Non-secretory protein with signalpeptide probability:0.141 (SignalP predicted) Absence of TMH's; Family membership; esterase 1103373 ychK 4606670 ychK Azoarcus sp. BH72 esterase YP_932530.1 1102525 D 62928 CDS YP_932531.1 119897318 4610160 1103397..1104803 1 NC_008702.1 Putative glycolate oxidase subunit GlcD. Homology to glcD of E. coli of 32% (sprot|GLCD_ECOLI). InterPro: FAD linked oxidase C-terminal (IPR004113);m FAD linked oxidas, N-terminal (IPR006093) Pfam: FAD binding domain; FAD linked oxidase, C-terminal domain Tigrfam: glcD: glycolate oxidase subunit GlcD no signal peptide no TMHs; Family membership; putative glycolate oxidase subunit GlcD 1104803 glcD2 4610160 glcD2 Azoarcus sp. BH72 putative glycolate oxidase subunit GlcD YP_932531.1 1103397 D 62928 CDS YP_932532.1 119897319 4609988 1105799..1106650 1 NC_008702.1 converts (S)-3-hydroxybutanoyl-CoA to 3-acetoacetyl-CoA; 3-hydroxybutyryl-CoA dehydrogenase 1106650 hbdA 4609988 hbdA Azoarcus sp. BH72 3-hydroxybutyryl-CoA dehydrogenase YP_932532.1 1105799 D 62928 CDS YP_932533.1 119897320 4608978 complement(1106727..1108178) 1 NC_008702.1 An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes; transcription termination factor Rho 1108178 rho 4608978 rho Azoarcus sp. BH72 transcription termination factor Rho YP_932533.1 1106727 R 62928 CDS YP_932534.1 119897321 4609719 complement(1108162..1108488) 1 NC_008702.1 Thioredoxin-disulfide reductase. Homology to trxA of T. ferrooxidans of 69% (THIO_THIFE). Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide. InterPro: Thioredoxin (IPR006662) Pfam: Thioredoxin no signal peptide no TMHs; Specificity unclear; thioredoxin-disulfide reductase 1108488 trxA1 4609719 trxA1 Azoarcus sp. BH72 thioredoxin-disulfide reductase YP_932534.1 1108162 R 62928 CDS YP_932535.1 119897322 4610039 1108784..1109107 1 NC_008702.1 Probable ferredoxin. Homology to fdxA of A. vineladii of 66% (sprot|FER1_AZOVI(SRS)). FERREDOXINS ARE IRON-SULFUR PROTEINS THAT TRANSFER ELECTRONS IN A WIDE VARIETY OF METABOLIC REACTIONS. THIS FERREDOXIN COULD PLAY A ROLE IN REGULATING GENE EXPRESSION BY INTERACTING DIRECTLY WITH DNA. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain IPR001450); 7Fe ferredoxin (IPR000813) Pfam: 4Fe-4S binding domain no signal peptide no TMHs; High confidence in function and specificity; ferredoxin 1109107 fdxA 4610039 fdxA Azoarcus sp. BH72 ferredoxin YP_932535.1 1108784 D 62928 CDS YP_932536.1 119897323 4608747 1109179..1109628 1 NC_008702.1 TREMBL:Q8XZ43; 52% identity, 70% similarity. TREMBL:Q8F5Y8; 30% identity, 56% similarity. Protein At5g10860 mitochondrial precursor. IPR000644: CBS domain Pfam:PF00571 - GARS_N TIGRFAM: rrf2 family, KpsF/GutQ family (by similarity) kpsF: KpsF/GutQ family protein; Specificity unclear; hypothetical protein 1109628 4608747 azo1032 Azoarcus sp. BH72 hypothetical protein YP_932536.1 1109179 D 62928 CDS YP_932537.1 119897324 4606671 1109767..1111458 1 NC_008702.1 Esterification concomitant with transport of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids, TREMBL:Q7NY16 (61% identity); TREMBL:Q7WLG0 (63% identity). InterPro (IPR000873): AMP-dependent synthetase and ligase Pfam (PF00501): AMP-binding enzyme.; High confidence in function and specificity; long-chain-fatty-acid-CoA ligase 1111458 fadD1 4606671 fadD1 Azoarcus sp. BH72 long-chain-fatty-acid-CoA ligase YP_932537.1 1109767 D 62928 CDS YP_932538.1 119897325 4608722 1111730..1113397 1 NC_008702.1 Acetolactate synthase isozyme I large subunit (AHAS-I) (Acetohydroxy-acid synthase I large subunit) (ALS-I). InterPro: Acetolactate synthase large subunit biosynthetic type acolac_lg: acetolactate synthase large subunit,biosynthetic type; High confidence in function and specificity; acetolactate synthase catalytic subunit 1113397 ilvB 4608722 ilvB Azoarcus sp. BH72 acetolactate synthase catalytic subunit YP_932538.1 1111730 D 62928 CDS YP_932539.1 119897326 4609069 1113400..1113714 1 NC_008702.1 with IlvB catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase small subunit; acetolactate synthase 1 regulatory subunit 1113714 ilvN 4609069 ilvN Azoarcus sp. BH72 acetolactate synthase 1 regulatory subunit YP_932539.1 1113400 D 62928 CDS YP_932540.1 119897327 4609076 1113825..1114961 1 NC_008702.1 catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis; chorismate synthase 1114961 aroC 4609076 aroC Azoarcus sp. BH72 chorismate synthase YP_932540.1 1113825 D 62928 CDS YP_932541.1 119897328 4608344 1114958..1116148 1 NC_008702.1 Putative transport protein. Similarity to sugar transporters (lacY): lactose permease 2A0105.Similar to POT family of proteins with DUF domains and 12 transmembrane helices. 31% identity and 47% similarity to putative MFS metabolite transporter from Pseudomonas aeruginosa PA01; Family membership; putative MFS metabolite transporter 1116148 4608344 azo1037 Azoarcus sp. BH72 putative MFS metabolite transporter YP_932541.1 1114958 D 62928 CDS YP_932542.1 119897329 4606672 1116163..1116984 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bb2112 of B. bronchiseptica of 55% (trembl|Q7WKJ4). Pfam: Peptidase family M48 signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 1116984 4606672 azo1038 Azoarcus sp. BH72 hypothetical protein YP_932542.1 1116163 D 62928 CDS YP_932543.1 119897330 4606673 1117079..1118488 1 NC_008702.1 dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate; isopropylmalate isomerase large subunit 1118488 leuC 4606673 leuC Azoarcus sp. BH72 isopropylmalate isomerase large subunit YP_932543.1 1117079 D 62928 CDS YP_932544.1 119897331 4609132 1118505..1119143 1 NC_008702.1 catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D; isopropylmalate isomerase small subunit 1119143 leuD 4609132 leuD Azoarcus sp. BH72 isopropylmalate isomerase small subunit YP_932544.1 1118505 D 62928 CDS YP_932545.1 119897332 4609133 1119180..1120244 1 NC_008702.1 catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis; 3-isopropylmalate dehydrogenase 1120244 leuB 4609133 leuB Azoarcus sp. BH72 3-isopropylmalate dehydrogenase YP_932545.1 1119180 D 62928 CDS YP_932546.1 119897333 4609131 1120327..1121427 1 NC_008702.1 catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde; aspartate-semialdehyde dehydrogenase 1121427 asd 4609131 asd Azoarcus sp. BH72 aspartate-semialdehyde dehydrogenase YP_932546.1 1120327 D 62928 CDS YP_932547.1 119897334 4608357 1121643..1124558 1 NC_008702.1 In Pseudomonas aeruginosa, FimV ist probable involved in remodelling of the peptidoglycan layer to enable assembly of the type IV fimbrial structure and machinery. And it is also required for twitching motility. Similar to trembl|O87015 (28%). Pfam (PF01476): LysM domain Pfam (PF04102): SlyX, may be a coiled-coil structure SignalP reporting Signal peptide; Function unclear; putative type 4 pilus biogenesis 1124558 fimV1 4608357 fimV1 Azoarcus sp. BH72 putative type 4 pilus biogenesis YP_932547.1 1121643 D 62928 CDS YP_932548.1 119897335 4608764 1124621..1125232 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA4769 of Azoarcus sp. EbN1 of 53% (gnl|keqq|eba:ebA4769(KEGG)). Pfam: Cobalt transport protein. signal peptide. 3 TMHs; Family membership; hypothetical protein 1125232 4608764 azo1044 Azoarcus sp. BH72 hypothetical protein YP_932548.1 1124621 D 62928 CDS YP_932549.1 119897336 4606674 1125226..1126023 1 NC_008702.1 mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability; tRNA pseudouridine synthase A 1126023 truA 4606674 truA Azoarcus sp. BH72 tRNA pseudouridine synthase A YP_932549.1 1125226 D 62928 CDS YP_932550.1 119897337 4606675 1126024..1126647 1 NC_008702.1 catalyzes the formation of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate from N-(5-phospho-beta-D-ribosyl)-anthranilate in tryptophan biosynthesis; N-(5'-phosphoribosyl)anthranilate isomerase 1126647 trpF 4606675 trpF Azoarcus sp. BH72 N-(5'-phosphoribosyl)anthranilate isomerase YP_932550.1 1126024 D 62928 CDS YP_932551.1 119897338 4610034 1126595..1127848 1 NC_008702.1 catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate; tryptophan synthase subunit beta 1127848 trpB 4610034 trpB Azoarcus sp. BH72 tryptophan synthase subunit beta YP_932551.1 1126595 D 62928 CDS YP_932552.1 119897339 4610030 1127891..1128706 1 NC_008702.1 catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis; tryptophan synthase subunit alpha 1128706 trpA 4610030 trpA Azoarcus sp. BH72 tryptophan synthase subunit alpha YP_932552.1 1127891 D 62928 CDS YP_932553.1 119897340 4610029 1128703..1129575 1 NC_008702.1 Function:-THIS PROTEIN IS A COMPONENT OF THE ACETYL COENZYME A CARBOXYLASE COMPLEX; FIRST BIOTIN CARBOXYLASE CATALYZES THE CARBOXYLATION OF THE CARRIER PROTEIN AND THEN THE TRANSCARBOXYLASE TRANSFERS THE CARBOXYL GROUP TO FORM MALONYL-COA (BY SIMILARITY). Entry name :-SWISSPROT:ACCD_ECOLI Prim. accession # P08193 Identities = 178/287 (62%) InterPro:- IPR000438; ACoACC_transB. IPR000022; Carboxyl_trans. Pfam:- PF01039; Carboxyl_trans; 1. Signal peptide probability: 0.000 Number of predicted TMHs: 0; High confidence in function and specificity; acetyl-coenzyme A carboxylase carboxyl transferase subunit b 1129575 accD 4610029 accD Azoarcus sp. BH72 acetyl-coenzyme A carboxylase carboxyl transferase subunit b YP_932553.1 1128703 D 62928 CDS YP_932554.1 119897341 4608258 1129592..1130890 1 NC_008702.1 FolC bifunctional protein [Includes: Folylpolyglutamate synthase (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS); Dihydrofolate synthase (EC 6.3.2.12)]. Conversion of folates to polyglutamate derivatives.; High confidence in function and specificity; dihydrofolate synthase/tetrahydrofolylpolyglutamate synthase 1130890 folC 4608258 folC Azoarcus sp. BH72 dihydrofolate synthase/tetrahydrofolylpolyglutamate synthase YP_932554.1 1129592 D 62928 CDS YP_932555.1 119897342 4608819 1130902..1131543 1 NC_008702.1 Conserved hypothetical membrane proteine. Homology to BB2598 of Bordetella bronchiseptica of 37% (trembl|Q7WJA1(SRS)). Has 2 copies of PF05036, Sporulation related repeat;IPR007730, SPOR; This 35 residue repeat is found in proteins involved in sporulation and cell division such as FtsN, DedD, and CwlM. This repeat might be involved in binding peptidoglycan (Bateman A pers obs). FtsN is an essential cell division protein with a simple bitopic topology, a short N-terminal cytoplasmic segment fused to a large carboxy periplasmic domain through a single transmembrane domain. These repeats lay at the periplasmic C-terminus. FtsN localises to the septum ring complex. The CwlM gene is a cell wall hydrolase so this repeat may help localise the protein to the cell wall. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 1131543 4608819 azo1051 Azoarcus sp. BH72 hypothetical protein YP_932555.1 1130902 D 62928 CDS YP_932556.1 119897343 4606676 1131554..1132045 1 NC_008702.1 Colicin V production protein (dedE protein) (Pur regulon 18 kDa protein). REQUIRED FOR COLICIN V PRODUCTION FROM PLASMID INCFI COLV3-K30. TREMBL:Q82WH7: 29% identity,38% similarity InterPro:IPR003825; Colicin_V. Pfam:PF02674; Colicin_V TIGR00304: conserved hypothetical protei Signal peptide present (Signal P predicted) TMH's 4 (TMHMM predicted); High confidence in function and specificity; putative colicin V production protein 1132045 cvpA 4606676 cvpA Azoarcus sp. BH72 putative colicin V production protein YP_932556.1 1131554 D 62928 CDS YP_932557.1 119897344 4608546 1132057..1133586 1 NC_008702.1 Catalyzes first step of the de novo purine nucleotide biosynthetic pathway; amidophosphoribosyltransferase 1133586 purF 4608546 purF Azoarcus sp. BH72 amidophosphoribosyltransferase YP_932557.1 1132057 D 62928 CDS YP_932558.1 119897345 4609659 1133594..1134763 1 NC_008702.1 catalyzes the conversion of O-succinylhomoserine into homocysteine; O-succinylhomoserine sulfhydrylase 1134763 metZ 4609659 metZ Azoarcus sp. BH72 O-succinylhomoserine sulfhydrylase YP_932558.1 1133594 D 62928 CDS YP_932559.1 119897346 4609213 complement(1134854..1135654) 1 NC_008702.1 catalyzes the formation of 2,3=diacylglucosamine 1-phosphate from UDP-2,3=diacylglucosamine; UDP-2,3-diacylglucosamine hydrolase 1135654 lpxH 4609213 lpxH Azoarcus sp. BH72 UDP-2,3-diacylglucosamine hydrolase YP_932559.1 1134854 R 62928 CDS YP_932560.1 119897347 4609178 complement(1135647..1136141) 1 NC_008702.1 Peptidyl-prolyl cis-trans isomerase B. Homology to ppiB of E.coli of 71% (sprot|PPIB_ECOLI). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. InterPro: Cyclophilin-type peptidyl-prolyl cis-trans isomerase(IPR002130) Pfam: Cyclophilin-type peptidyl-prolyl cis-trans isomerase no signal peptide no TMH; High confidence in function and specificity; peptidyl-prolyl cis-trans isomerase B 1136141 ppiB 4609178 ppiB Azoarcus sp. BH72 peptidyl-prolyl cis-trans isomerase B YP_932560.1 1135647 R 62928 CDS YP_932561.1 119897348 4609610 complement(1136158..1136739) 1 NC_008702.1 Probable peptidyl-prolyl cis-trans isomerase. Homology to ppiA of E. coli of 56% (SWISSPROT:PPIA_ECOLI) PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. InterPro: Cyclophilin-type peptidyl-prolyl cis-trans isomerase (IPR002130) Pfam: Cyclophilin-type peptidyl-prolyl cis-trans isomerase Signal peptide no TMHs; High confidence in function and specificity; peptidyl-prolyl cis-trans isomerase A 1136739 ppiA 4609610 ppiA Azoarcus sp. BH72 peptidyl-prolyl cis-trans isomerase A YP_932561.1 1136158 R 62928 CDS YP_932562.1 119897349 4609609 complement(1136784..1137980) 1 NC_008702.1 Conserved hypothetical secreted proteins. Homology to of Dechloromonas aromatica of 56% (gi|41723709|ref|ZP_00150619.1|(NBCI ENTREZ)). Signal Peptide Present. No TMH present. Has PF03734:(IPR005490)ErfK/YbiS/YcfS/YnhG;This family of proteins are found in a range of bacteria. The conserved region contains a conserved histidine and cysteine,suggesting that these proteins have an enzymatic activity. Several members of this family contain peptidoglycan binding domains. So these proteins may use peptidoglycan or a precursor as a substrate.; Conserved hypothetical protein; hypothetical protein 1137980 4609609 azo1058 Azoarcus sp. BH72 hypothetical protein YP_932562.1 1136784 R 62928 CDS YP_932563.1 119897350 4606677 complement(1137983..1139239) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002816 of Dechloromonas aromatica of 42% (gi|53730051|ref|ZP_00348646.1|(NBCI ENTREZ)). domain structure: 59 aa - 92 aa TRP; 93 aa- 126 aa TRP; 127 aa -155 aa TRP. InterPro: TPR repeat (IPR001440); Type I antifreeze protein (IPR000104). Pfam: TRP Domain. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 1139239 4606677 azo1059 Azoarcus sp. BH72 hypothetical protein YP_932563.1 1137983 R 62928 CDS YP_932564.1 119897351 4606678 1139410..1140792 1 NC_008702.1 catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase 1140792 cysS 4606678 cysS Azoarcus sp. BH72 cysteinyl-tRNA synthetase YP_932564.1 1139410 D 62928 CDS YP_932565.1 119897352 4608564 complement(1140857..1142011) 1 NC_008702.1 This family includes extracellular ligand binding domains of a wide range of receptors and it also includes the bacterial amino acid binding proteins of known structure. Similar to trembl|Q8XQ39 (40%) and to trembl|Q89G00 (32%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Family membership; extracellular ligand binding protein 1142011 4608564 azo1061 Azoarcus sp. BH72 extracellular ligand binding protein YP_932565.1 1140857 R 62928 CDS YP_932566.1 119897353 4606679 complement(1142201..1143328) 1 NC_008702.1 chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; chaperone protein DnaJ 1143328 dnaJ1 4606679 dnaJ1 Azoarcus sp. BH72 chaperone protein DnaJ YP_932566.1 1142201 R 62928 CDS YP_932567.1 119897354 4608634 complement(1143424..1145349) 1 NC_008702.1 heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria; molecular chaperone DnaK 1145349 dnaK 4608634 dnaK Azoarcus sp. BH72 molecular chaperone DnaK YP_932567.1 1143424 R 62928 CDS YP_932568.1 119897355 4608636 complement(1145464..1146030) 1 NC_008702.1 with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor; heat shock protein GrpE 1146030 grpE 4608636 grpE Azoarcus sp. BH72 heat shock protein GrpE YP_932568.1 1145464 R 62928 CDS YP_932569.1 119897356 4608942 1146167..1146412 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Polaromonas sp. JS666 of 43% ( ZP_00361754). No domains predicted. No singal peptide. No TMHs; hypothetical protein 1146412 4608942 azo1065 Azoarcus sp. BH72 hypothetical protein YP_932569.1 1146167 D 62928 CDS YP_932570.1 119897357 4606680 1146520..1149153 1 NC_008702.1 negatively supercoils closed circular double-stranded DNA; DNA gyrase subunit A 1149153 gyrA 4606680 gyrA Azoarcus sp. BH72 DNA gyrase subunit A YP_932570.1 1146520 D 62928 CDS YP_932571.1 119897358 4608975 1149150..1150247 1 NC_008702.1 catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine; phosphoserine aminotransferase 1150247 serC 4608975 serC Azoarcus sp. BH72 phosphoserine aminotransferase YP_932571.1 1149150 D 62928 CDS YP_932572.1 119897359 4609867 1150262..1151326 1 NC_008702.1 Bifunctional chorismate mutase/prephenate dehydratase P-protein, pheA,; High confidence in function and specificity; chorismate mutase/prephenate dehydratase 1151326 pheA 4609867 pheA Azoarcus sp. BH72 chorismate mutase/prephenate dehydratase YP_932572.1 1150262 D 62928 CDS YP_932573.1 119897360 4609544 1151344..1152441 1 NC_008702.1 catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis; histidinol-phosphate aminotransferase 1152441 hisC1 4609544 hisC1 Azoarcus sp. BH72 histidinol-phosphate aminotransferase YP_932573.1 1151344 D 62928 CDS YP_932574.1 119897361 4609001 1152457..1153347 1 NC_008702.1 Prephenate dehydrogenases are involved in tyrosine biosynthesis. Similar to trembl|Q7NSL5 (54%) and to trembl|Q82XD9 (47%). Pfam: PDH, Prephenate dehydrogenase. TMHMM reporting one Tmhelix. SignalP reporting Signal peptide.; Specificity unclear; prephenate dehydrogenase 1153347 tyrA 4609001 tyrA Azoarcus sp. BH72 prephenate dehydrogenase YP_932574.1 1152457 D 62928 CDS YP_932575.1 119897362 4610054 1153344..1155305 1 NC_008702.1 catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis; catalyzes CMP phosphorylation; bifunctional 3-phosphoshikimate 1-carboxyvinyltransferase/cytidine monophosphate kinase 1155305 aroA 4610054 aroA Azoarcus sp. BH72 bifunctional 3-phosphoshikimate 1-carboxyvinyltransferase/cytidine monophosphate kinase YP_932575.1 1153344 D 62928 CDS YP_932576.1 119897363 4608342 1155409..1157115 1 NC_008702.1 in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins; 30S ribosomal protein S1 1157115 rpsA 4608342 rpsA Azoarcus sp. BH72 30S ribosomal protein S1 YP_932576.1 1155409 D 62928 CDS YP_932577.1 119897364 4609805 1157126..1157410 1 NC_008702.1 This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control; integration host factor subunit beta 1157410 ihfB 4609805 ihfB Azoarcus sp. BH72 integration host factor subunit beta YP_932577.1 1157126 D 62928 CDS YP_932578.1 119897365 4609067 1157501..1157806 1 NC_008702.1 38% identity with Hypothetical protein from Chromobacterium violaceum TrEMBL:Q7NTL0,Genename CV3044 Signal Peptide present TMHMM reporting for transmembrane helices:2 present.; Conserved hypothetical protein; hypothetical protein 1157806 4609067 azo1074 Azoarcus sp. BH72 hypothetical protein YP_932578.1 1157501 D 62928 CDS YP_932579.1 119897366 4606681 1157814..1158989 1 NC_008702.1 Putative N-acetylglucosaminyl transferase. Enzyme that is involved in the catalysis of the reaction: UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N''-diacetylchitobiosyldiphosphodolichol. 36% Cas_TM1810_C.IPR001574; RIP.IPR001440; TPR.IPR008941; TPR-like.InterPro: TPR repeat TIGRFAMs:TIGR01870; cas_TM1810; 1. TMHx:1; Specificity unclear; tetratricopeptide repeat protein 1158989 4606681 azo1075 Azoarcus sp. BH72 tetratricopeptide repeat protein YP_932579.1 1157814 D 62928 CDS YP_932580.1 119897367 4606682 1159107..1160429 1 NC_008702.1 UDP-glucose 6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase) (UDP-GlcDH) (UDPGDH).; High confidence in function and specificity; UDP-glucose 6-dehydrogenase 1160429 ugd 4606682 ugd Azoarcus sp. BH72 UDP-glucose 6-dehydrogenase YP_932580.1 1159107 D 62928 CDS YP_932581.1 119897368 4610064 1160583..1161473 1 NC_008702.1 catalyzes the formation of cysteine from 3-O-acetyl-L-serine and hydrogen sulfide; cysteine synthase B 1161473 cysM 4610064 cysM Azoarcus sp. BH72 cysteine synthase B YP_932581.1 1160583 D 62928 CDS YP_932582.1 119897369 4608561 1161512..1162306 1 NC_008702.1 Probable DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A). TIGR00099: conserved hypothetical protein.; Function unclear; hypothetical protein 1162306 4608561 azo1078 Azoarcus sp. BH72 hypothetical protein YP_932582.1 1161512 D 62928 CDS YP_932583.1 119897370 4610025 1162660..1164576 1 NC_008702.1 catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); threonyl-tRNA synthetase 1164576 thrS 4610025 thrS Azoarcus sp. BH72 threonyl-tRNA synthetase YP_932583.1 1162660 D 62928 CDS YP_932584.1 119897371 4606684 1164657..1165127 1 NC_008702.1 IF-3 has several functions that are required and promote translation initiation including; preventing association of 70S by binding to 30S; monitoring codon-anticodon interactions by promoting disassociation of fMet-tRNA(fMet) from initiation complexes formed on leaderless mRNAs or incorrectly bound noninitiatior tRNAs and complexes with noncanonical start sites; stimulates codon-anticodon interactions at P-site; involved in moving mRNA to the P-site; and in recycling subunits; translation initiation factor IF-3 1165127 infC 4606684 infC Azoarcus sp. BH72 translation initiation factor IF-3 YP_932584.1 1164657 D 62928 CDS YP_932585.1 119897372 4609084 1165275..1165472 1 NC_008702.1 50S ribosomal protein L35.; Family membership; 50S ribosomal protein L35 1165472 rpmI 4609084 rpmI Azoarcus sp. BH72 50S ribosomal protein L35 YP_932585.1 1165275 D 62928 CDS YP_932586.1 119897373 4609792 1165485..1165844 1 NC_008702.1 binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit; 50S ribosomal protein L20 1165844 rplT 4609792 rplT Azoarcus sp. BH72 50S ribosomal protein L20 YP_932586.1 1165485 D 62928 CDS YP_932587.1 119897374 4609779 1165911..1166942 1 NC_008702.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily; phenylalanyl-tRNA synthetase subunit alpha 1166942 pheS 4609779 pheS Azoarcus sp. BH72 phenylalanyl-tRNA synthetase subunit alpha YP_932587.1 1165911 D 62928 CDS YP_932588.1 119897375 4609546 1166949..1169339 1 NC_008702.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily; phenylalanyl-tRNA synthetase subunit beta 1169339 pheT 4609546 pheT Azoarcus sp. BH72 phenylalanyl-tRNA synthetase subunit beta YP_932588.1 1166949 D 62928 CDS YP_932589.1 119897376 4609547 1169336..1169647 1 NC_008702.1 This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control; integration host factor subunit alpha 1169647 ihfA 4609547 ihfA Azoarcus sp. BH72 integration host factor subunit alpha YP_932589.1 1169336 D 62928 CDS YP_932590.1 119897377 4609066 1169628..1170008 1 NC_008702.1 Putative MerR-family transcriptional regulator,similar toTREMBL: trembl|Q83C17 (51% Coxiella burnetii,cbu1319) Pfam: PF00376 MerR family regulatory protein. HTH reporting nucleic acid binding motif.; MerR family transcriptional regulator 1170008 4609066 azo1086 Azoarcus sp. BH72 MerR family transcriptional regulator YP_932590.1 1169628 D 62928 CDS YP_932591.1 119897378 4610014 1170324..1171067 1 NC_008702.1 catalyzes the conversion of a phosphate monoester to an alcohol and a phosphate; stationary phase survival protein SurE 1171067 surE 4610014 surE Azoarcus sp. BH72 stationary phase survival protein SurE YP_932591.1 1170324 D 62928 CDS YP_932592.1 119897379 4609911 1171112..1171717 1 NC_008702.1 Protein-L-isoaspartate O-methyltransferase, 56% Identity to TrEMBL;Q7VXN1.51% Identity to TrEMBL;Q82VW0,Q7NRV0. SProt;Q886L4(52% Idnetity), Q87LQ6(51%),P24206(47%) Has PF01135, Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT);IPR000682, PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) MEDLINE: (which is also known as L-isoaspartyl protein carboxyl methyltransferase) is an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to the free carboxyl groups of D-aspartyl or L-isoaspartyl residues in a variety of peptides and proteins. The enzyme does not act on normal L-aspartyl residues L-isoaspartyl and D-aspartyl are the products of the spontaneous deamidation and/or isomerization of normal L-aspartyl and L- asparaginyl residues in proteins. PCMT plays a role in the repair and/or degradation of these damaged proteins; the enzymatic methyl esterification of the abnormal residues can lead to their conversion to normal L-aspartyl residues.; protein-L-isoaspartate (D-aspartate) O-methyltransferase 1171717 pcm2 4609911 pcm2 Azoarcus sp. BH72 protein-L-isoaspartate (D-aspartate) O-methyltransferase YP_932592.1 1171112 D 62928 CDS YP_932593.1 119897380 4609511 1171714..1172622 1 NC_008702.1 Conserved hypothetical lipoprotein. Homology to nlpD of R. gelatinosus of 43% (trembl|Q9JP90) InterPro: Peptidase family M23/M37 (IPR002886); LysM motif (IPR002482) Pfam: LysM domain; Peptidase family m23/M27 signal peptide no TMHs; Conserved hypothetical protein; lipoprotein 1172622 nlpD 4609511 nlpD Azoarcus sp. BH72 lipoprotein YP_932593.1 1171714 D 62928 CDS YP_932594.1 119897381 4609384 1172633..1173568 1 NC_008702.1 sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor controls a regulon of genes required for protection against external stresses; RNA polymerase sigma factor RpoS 1173568 rpoS 4609384 rpoS Azoarcus sp. BH72 RNA polymerase sigma factor RpoS YP_932594.1 1172633 D 62928 CDS YP_932595.1 119897382 4609802 complement(1173565..1174575) 1 NC_008702.1 Putative membrane-bound lytic murein transglycosylase. homology to mltb of E. coli of 38% (sprot|MLTB_ECOLI) Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division. no domains prediced signal peptide no TMHs; Family membership; putative membrane-bound lytic murein transglycosylase 1174575 mltB 4609802 mltB Azoarcus sp. BH72 putative membrane-bound lytic murein transglycosylase YP_932595.1 1173565 R 62928 CDS YP_932596.1 119897383 4609228 complement(1174572..1176551) 1 NC_008702.1 Conserved hypotetical membrane protein. Homology to RSp0111 of R. solanacearum of 41% (trembl|Q8XTJ7(SRS)) Pfam: Transglutaminase-like superfamily signal peptide probable 4 TMHs; Conserved hypothetical protein; hypothetical protein 1176551 4609228 azo1092 Azoarcus sp. BH72 hypothetical protein YP_932596.1 1174572 R 62928 CDS YP_932597.1 119897384 4606686 complement(1176544..1177527) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS03012 of Ralstonia solanacearum of 40% (trembl|Q8XTJ8) InterPro: Protein of unknown function DUF58 This domain is found in a family of prokaryotic proteins that have no known function. Proteins belonging to this family include hypothetical proteins from eubacteria and archaebacteria. Some of these proteins also contain the Von Willebrand factor, type A domain InterterPro: IPR002881; DUF58. Pfam: PF01882; DUF58; Non-secretory with low signal peptide probability (Signal P predicted). TMHMM predicted 2 transmembrane helices.; Conserved hypothetical protein; hypothetical protein 1177527 4606686 azo1093 Azoarcus sp. BH72 hypothetical protein YP_932597.1 1176544 R 62928 CDS YP_932598.1 119897385 4606687 complement(1177527..1178444) 1 NC_008702.1 MoxR protein (MxaR protein). MAY BE INVOLVED IN THE REGULATION OF FORMATION OF ACTIVE METHANOL DEHYDROGENASE. TREMBL:Q883V0: 60% identity, 73% similarity SMART: SM00382; AAA InterPro: IPR003593; AAA_ATPase. IPR001270; Chaprnin_clpA/B. IPR003442; UPF0079. Pfam: PF02367; UPF0079; Pfam: Mg_chelatase:Magnesium chelatase, subunit ChlI ruvB: Holliday junction DNA helicase Ru No signal peptide and transmembrane helices; High confidence in function and specificity; MoxR protein 1178444 moxR1 4606687 moxR1 Azoarcus sp. BH72 MoxR protein YP_932598.1 1177527 R 62928 CDS YP_932599.1 119897386 4609264 1178599..1179525 1 NC_008702.1 Histone deacetylases, acetoin utilization proteins and acetylpolyamine amidohydrolases are all members of an ancient protein superfamily, TREMBL:Q7VZF1 (58% identity); TREMBL:Q7NRU4 (57% identity). InterPro (IPR000286): Histone deacetylase family Pfam (PF00850): Histone deacetylase domain.; Family membership; histone deacetylase family protein 1179525 4609264 azo1095 Azoarcus sp. BH72 histone deacetylase family protein YP_932599.1 1178599 D 62928 CDS YP_932600.1 119897387 4606688 1179632..1180510 1 NC_008702.1 catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate synthase 1180510 dapA 4606688 dapA Azoarcus sp. BH72 dihydrodipicolinate synthase YP_932600.1 1179632 D 62928 CDS YP_932601.1 119897388 4608580 1180537..1181676 1 NC_008702.1 Putative lipoprotein. Homology to nlpB of E. coli of 20% (sprot|NLPB_ECOLI) This family consists of a number of bacterial lipoproteins often known as NlpB or DapX. This lipoprotein is detected in outer membrane vesicles in Escherichia coli and appears to be nonessential. Pfam: NlpB/DapX lipoprotein singal peptide no TMHs; Family membership; putative lipoprotein 1181676 nlpB 4608580 nlpB Azoarcus sp. BH72 putative lipoprotein YP_932601.1 1180537 D 62928 CDS YP_932602.1 119897389 4609383 complement(1181789..1182073) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NMB1523 of N.meningitidis of 50% (tremble:Q9JYL7) No domains predicted. No TMHs Signal Peptide present.; Conserved hypothetical protein; hypothetical protein 1182073 4609383 azo1098 Azoarcus sp. BH72 hypothetical protein YP_932602.1 1181789 R 62928 CDS YP_932603.1 119897390 4606689 complement(1182332..1183447) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3581 of C.violaceum of 48% (tremble:Q7NS46) SM00558;JmjC:Probable enzymes, but of unknown functions,that regulate chromatin reorganisation processes. No signal peptide or TMH present. lytR_cpsA_psr: cell envelope-related fu; hypothetical protein 1183447 4606689 azo1099 Azoarcus sp. BH72 hypothetical protein YP_932603.1 1182332 R 62928 CDS YP_932604.1 119897391 4606690 1183539..1184840 1 NC_008702.1 in Escherichia coli this enzyme catalyzes the SAM-dependent methylation of U1939 in the 23S ribomal RNA; binds an iron-sulfur cluster [4Fe-4S]; 23S rRNA 5-methyluridine methyltransferase 1184840 rumA 4606690 rumA Azoarcus sp. BH72 23S rRNA 5-methyluridine methyltransferase YP_932604.1 1183539 D 62928 CDS YP_932605.1 119897392 4610016 1185090..1185695 1 NC_008702.1 Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA; Family membership; pseudouridylate synthase 1185695 4610016 azo1101 Azoarcus sp. BH72 pseudouridylate synthase YP_932605.1 1185090 D 62928 CDS YP_932606.1 119897393 4606692 1185730..1186185 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc2494 of R. solanacearum of 48% (trembl|Q8XWI0(SRS)). Pfam: DUF192 signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 1186185 4606692 azo1102 Azoarcus sp. BH72 hypothetical protein YP_932606.1 1185730 D 62928 CDS YP_932607.1 119897394 4606693 1186321..1187466 1 NC_008702.1 membrane protein responsible for substrate specificity switching from rod/hook-type export to filament-type export; flagellar biosynthesis protein FlhB 1187466 flhB 4606693 flhB Azoarcus sp. BH72 flagellar biosynthesis protein FlhB YP_932607.1 1186321 D 62928 CDS YP_932608.1 119897395 4608790 1187481..1189580 1 NC_008702.1 membrane protein involved in the flagellar export apparatus; flagellar biosynthesis protein FlhA 1189580 flhA 4608790 flhA Azoarcus sp. BH72 flagellar biosynthesis protein FlhA YP_932608.1 1187481 D 62928 CDS YP_932609.1 119897396 4608789 1189724..1191256 1 NC_008702.1 positive regulator of class III flagellar genes; flagellar biosynthesis regulator FlhF 1191256 flhF 4608789 flhF Azoarcus sp. BH72 flagellar biosynthesis regulator FlhF YP_932609.1 1189724 D 62928 CDS YP_932610.1 119897397 4608793 1191259..1192041 1 NC_008702.1 Hypothetical flagellar related protein FleN. Homology to fleN of Pseudomonas fluorescens of 27% (AAN03366). No domains predicted. No signal peptide. No TMHs; flagellar-like protein FleN 1192041 fleN 4608793 fleN Azoarcus sp. BH72 flagellar-like protein FleN YP_932610.1 1191259 D 62928 CDS YP_932611.1 119897398 4608773 1192166..1192936 1 NC_008702.1 RNA polymerase sigma factor for flagellar operon (Sigma-F factor) (Sigma-27) (Sigma-28). THE SIGMA FACTOR IS AN INITIATION FACTOR THAT PROMOTES ATTACHMENT OF THE RNA POLYMERASE TO SPECIFIC INITIATION SITES AND THEN IS RELEASED. THIS ALTERNATIVE SIGMA FACTOR IS SPECIFIC FOR CLASS 3 FLAGELLAR OPERONS. Similar to SWISSPROT: sprot|FLIA_PSEAE (46% Pseudomonas aeruginosa, RNA polymerase sigma factor for flagellar operon (sigma-f factor) (sigma-28)). InterPro: IPR000943 Sigma_70. Pfam: PF00140 Sigma-70 factor, region 1.2. HTH reporting nucleic acid binding motif. gatB_rel: aspartyl-tRNA(Asn) amidotrans; High confidence in function and specificity; RNA polymerase sigma factor 1192936 fliA 4608773 fliA Azoarcus sp. BH72 RNA polymerase sigma factor YP_932611.1 1192166 D 62928 CDS YP_932612.1 119897399 4608794 1193004..1193744 1 NC_008702.1 homologous to MotA; this protein with a related protein (a MotB homolog) forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine; either MotAB or MotCD is sufficient for swimming, but both are necessary for swarming motility; these organisms have both MotA and MotC; flagellar motor protein 1193744 motC 4608794 motC Azoarcus sp. BH72 flagellar motor protein YP_932612.1 1193004 D 62928 CDS YP_932613.1 119897400 4606694 complement(1193801..1195465) 1 NC_008702.1 E. coli uses five MCP-family receptors to promote chemotactic movements toward different attractant compounds: Tar is the aspartate and maltose chemoreceptor. Methyl-accepting chemotaxis protein,; Specificity unclear; putative aspartate chemoreceptor protein 1195465 tar 4606694 tar Azoarcus sp. BH72 putative aspartate chemoreceptor protein YP_932613.1 1193801 R 62928 CDS YP_932614.1 119897401 4609916 1195645..1196484 1 NC_008702.1 Homologous to MotB. These organism have both MotB and MotD. With MotC (a MotA homolog) forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine. Either MotAB or MotCD is sufficient for swimming, but both are necessary for swarming motility; flagellar motor protein MotD 1196484 motB1 4609916 motB1 Azoarcus sp. BH72 flagellar motor protein MotD YP_932614.1 1195645 D 62928 CDS YP_932615.1 119897402 4609262 1196592..1197074 1 NC_008702.1 DcrH:hemerythrin protein, is transmembrane methyl-accepting protein probably involved in bacterial chemotaxis . 31% Hemerythrin. Pfam:PF01814; Hemerythrin; 1.; High confidence in function and specificity; putative hemerythrin-like protein 1197074 dcrH2 4609262 dcrH2 Azoarcus sp. BH72 putative hemerythrin-like protein YP_932615.1 1196592 D 62928 CDS YP_932616.1 119897403 4608591 complement(1197195..1199600) 1 NC_008702.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit A 1199600 4608591 azo1112 Azoarcus sp. BH72 DNA topoisomerase IV subunit A YP_932616.1 1197195 R 62928 CDS YP_932617.1 119897404 4606695 complement(1199800..1200315) 1 NC_008702.1 Conserved hypothetical protein. Homology to Bucepa03000705 of Burkholderia cepacia of 33% (gi|46323973|ref|ZP_00224335.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1200315 4606695 azo1113 Azoarcus sp. BH72 hypothetical protein YP_932617.1 1199800 R 62928 CDS YP_932618.1 119897405 4606696 complement(1200393..1201181) 1 NC_008702.1 Phospholysine phosphohistidine inorganic pyrophosphate phosphatase. Enzyme that hydrolyzes not only oxygen-phosphorus bonds in inorganic pyrophosphate but also nitrogen-phosphorus bonds in phospholysine,phosphohistidine and imidodiphosphate in vitro. 38% HAD_SF_IIA.IPR006355; HAD_SF_IIA_hyp2.IPR005834; Hydrolase. Pfam:PF00702;Hydrolase; 1. TIGRFAMs:TIGR01460; HAD-SF-IIA; 1.TIGR01458; HAD-SF-IIA-hyp3; 1. TMHelix:1; High confidence in function and specificity; putative inorganic pyrophosphate phosphatase 1201181 lhpp 4606696 lhpp Azoarcus sp. BH72 putative inorganic pyrophosphate phosphatase YP_932618.1 1200393 R 62928 CDS YP_932619.1 119897406 4609137 complement(1201275..1203248) 1 NC_008702.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit B 1203248 parE 4609137 parE Azoarcus sp. BH72 DNA topoisomerase IV subunit B YP_932619.1 1201275 R 62928 CDS YP_932620.1 119897407 4609501 complement(1203341..1203955) 1 NC_008702.1 Conserved hypothetical membrane protein,TrEMBL;Q8NSJ9(49% identity,73% similarity),TrEMBL;Q7VSN7(53% identity, 72% similarity). Has 2 PF03458(IPR005115 ):UPF0126 domain;Domain always found as pair in bacterial membrane proteins of unknown function. This domain contains three transmembrane helices. The conserved glycines are suggestive of an ion channel (C. Yeats unpublished obs.). signal peptide 6 TMHs; Conserved hypothetical protein; hypothetical protein 1203955 4609501 azo1116 Azoarcus sp. BH72 hypothetical protein YP_932620.1 1203341 R 62928 CDS YP_932621.1 119897408 4606697 complement(1204519..1205832) 1 NC_008702.1 Isocitrate lyase. Homology to aceA of E. coli of 73% (sprot|ACEA_ECOLI). Isocitrate lyase is an enzyme that catalyzes the conversion of isocitrate to succinate and glyoxylate. This is the first step in the glyoxylate bypass, an alternative to the tricarboxylic acid cycle in bacteria, fungi and plants. InterPro: Isocitrate lyase (IPR000918) Pfam: Isocitrate lyase family no signal peptide no TMHs; High confidence in function and specificity; AceA protein 1205832 aceA 4606697 aceA Azoarcus sp. BH72 AceA protein YP_932621.1 1204519 R 62928 CDS YP_932622.1 119897409 4608259 1206325..1207365 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 1207365 4608259 azo1118 Azoarcus sp. BH72 AraC family transcriptional regulator YP_932622.1 1206325 D 62928 CDS YP_932623.1 119897410 4606698 1207554..1209134 1 NC_008702.1 Phytoene dehydrogenase (EC 1.14.99.-) (Phytoene desaturase). Phytoene dehydrogenase (phytoene desaturase) is an enzyme of carotenoid biosynthesis that converts phytoene into zeta-carotene via the symmetrical introduction of two double bonds at the C-11 and C-11' positions of phytoene, TREMBL:Q8XYC4 (50% identity); SWISSPROT:P06108 (26% identity). InterPro (IPR008150): Bacterial-type phytoene dehydrogenase Pfam (Phytoene_dh): Phytoene dehydrogenase related enzyme.; High confidence in function and specificity; phytoene dehydrogenase 1209134 crtI 4606698 crtI Azoarcus sp. BH72 phytoene dehydrogenase YP_932623.1 1207554 D 62928 CDS YP_932624.1 119897411 4608536 complement(1209318..1210130) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8XYC0 (36% identity); TREMBL:Q9EX74 (34% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short chain dehydrogenase 1210130 4608536 azo1120 Azoarcus sp. BH72 short chain dehydrogenase YP_932624.1 1209318 R 62928 CDS YP_932625.1 119897412 4606699 complement(1210127..1210732) 1 NC_008702.1 This is a family of hydrolase enzymes. Isochorismatase, also known as 2,3 dihydro-2,3 dihydroxybenzoatesynthase catalyses the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate, TREMBL:Q9HZE2 (34% identity); TREMBL:Q8XSM9 (39% identity). Pfam (PF00857): Isochorismatase family.; Family membership; isochorismatase family protein 1210732 4606699 azo1121 Azoarcus sp. BH72 isochorismatase family protein YP_932625.1 1210127 R 62928 CDS YP_932626.1 119897413 4606700 complement(1210885..1211421) 1 NC_008702.1 Ring hydroxylating dioxygenases are multicomponent 1,2-dioxygenase complexes that convert closed-ring structures to non-aromatic cis-diols. The beta subunit is may be responsible for the substrate specificity of the enzyme. Putative 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase, CarBa. Involved in the aerobic degradation of carbazole by P.resinovorans sp. strain CA10. XlyY: Benzene 12-dioxygenase beta subunit (EC 1.14.12.3), involved in benzoate degradation.Degradation of benzoate to 2-hydro-12-dihydroxybenzoate (dhb).; Conserved hypothetical protein; putative dioxygenase, hydroxylase small subunit 1211421 carBa1 4606700 carBa1 Azoarcus sp. BH72 putative dioxygenase, hydroxylase small subunit YP_932626.1 1210885 R 62928 CDS YP_932627.1 119897414 4608416 complement(1211408..1212763) 1 NC_008702.1 Ring hydroxylating dioxygenases are multicomponent 1,2-dioxygenase complexes that convert closed-ring structures to non-aromatic cis-diols. Hydroxylase large component of 1,2-dioxygenase complex, involved in aromatic compounds degradation. 81% simialr to a Putative 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase, CarBb. Involved in the aerobic degradation of carbazole by Pseudomonas resinovorans sp. strain CA10. TREMBL:Q8GI28 InterPro:IPR005806; Rieske_reg.IPR001663; Ring_hydroxyl_A. Pfam:PF00355; Rieske; 1. PF00848; Ring_hydroxyl_A; 1.; High confidence in function and specificity; putative dioxygenase hydroxylase large subunit 1212763 carBb1 4608416 carBb1 Azoarcus sp. BH72 putative dioxygenase hydroxylase large subunit YP_932627.1 1211408 R 62928 CDS YP_932628.1 119897415 4608418 complement(1212783..1213730) 1 NC_008702.1 Probable vanillate O-demethylase oxidoreductase (Vanillate degradation ferredoxin-like protein). Homology to vanB of P. sp HR199 of 40% (sprot|VANB_PSEUH). The vanillate demethylase (EC:1.14.13.82) consists of two proteins: an oxygenase and an oxygenase reductase (VanA and VanB). This enzyme is involved in the vanillate degradation, a key intermediate in the degradation of lignin. InterPro: NADH:cytochrome b5 reductase (CBR)(IPR001834); Ferredoxin (IPR001041); Oxidoreductase FAD and NAD(P) binding domain (IPR001433) Pfam: Oxidoredutase FAD-binding domain; Oxidoreductase NAD-binding domain; 2Fe-2S iron-sulfur cluster binding domain no signal peptide no TMHs; Family membership; vanillate O-demethylase oxidoreductase 1213730 vanB1 4608418 vanB1 Azoarcus sp. BH72 vanillate O-demethylase oxidoreductase YP_932628.1 1212783 R 62928 CDS YP_932629.1 119897416 4610090 1214284..1215204 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q7W060 (64%) and to sprot|LIVH_ECOLI (24%). Pfam (PF02653): Branched-chain amino acid transport system / permease component TMHMM reporting eight Tmhelix.; Specificity unclear; ABC transporter permease 1215204 4610090 azo1125 Azoarcus sp. BH72 ABC transporter permease YP_932629.1 1214284 D 62928 CDS YP_932630.1 119897417 4606701 1215207..1216199 1 NC_008702.1 Putative branched-chain amino acid transport permease Homology to livM of S. typhimurium of 28%. Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. InterPro: Binding-system dependent bacterial transporters (araH livH/limM families) Pfam: Branched-chain amino acid transport system signal peptide probable 9 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 1216199 livM2 4606701 livM2 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_932630.1 1215207 D 62928 CDS YP_932631.1 119897418 4609159 1216196..1216945 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP binding domains are responsible for coupling the energy of ATP hydrolysis to conformational changes in the ransmembrane domains. Similar to trembl|Q7W058 (49%) and to sprot|LIVG_ECOLI (39%). Pfam (PF00005): ABC transporter Smart (SM00382): AAA ATPase superfamily; Specificity unclear; ABC transporter ATP-binding protein 1216945 4609159 azo1127 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932631.1 1216196 D 62928 CDS YP_932632.1 119897419 4606702 1216942..1217658 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q7W057 (65%) and to trembl|Q84HI4 (49%). Smart (SM00382): ATPase superfamily Pfam (PF00005): ABC transporter ProSite (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; ABC transporter ATP-binding protein 1217658 4606702 azo1128 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_932632.1 1216942 D 62928 CDS YP_932633.1 119897420 4606703 1217770..1218957 1 NC_008702.1 ABC transporter substrate binding proteins counts to the family of extracellular ligand binding proteins. It is a component of the high affinity amino acid transport system. Similar to trembl|Q83V39 (51%) and to trembl|Q7WF58 (49%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Function unclear; ABC transporter substrate-binding protein 1218957 4606703 azo1129 Azoarcus sp. BH72 ABC transporter substrate-binding protein YP_932633.1 1217770 D 62928 CDS YP_932634.1 119897421 4606704 complement(1219045..1219530) 1 NC_008702.1 regulator of RNase E; increases half-life and abundance of RNAs; interacts with RNase E possibly inhibiting catalytic activity; ribonuclease activity regulator protein RraA 1219530 4606704 azo1130 Azoarcus sp. BH72 ribonuclease activity regulator protein RraA YP_932634.1 1219045 R 62928 CDS YP_932635.1 119897422 4606705 complement(1219713..1220327) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001685 of Dechloromonas aromatica of 46% (gi|41725028|ref|ZP_00151838.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1220327 4606705 azo1131 Azoarcus sp. BH72 hypothetical protein YP_932635.1 1219713 R 62928 CDS YP_932636.1 119897423 4606706 complement(1220421..1222703) 1 NC_008702.1 ATP-dependent Clp protease ATP-binding subunit clpA. Homology to clpA of E. coli of 63% (sprot|CLPA_ECOLI). ATP-dependent specificity component of the clpP protease. It directs the protease to specific substrates. The primary function of the clpA-clpP complex appears to be the degradation of unfolded or abnormal proteins. InterPro: AAA-protein (ATPases associated with various cellular activities)(IPR003959); AAA ATPase superfamily (IPR003593); Clp amino terminus (IPR004176); chaperonins clpA/B (IPR001270); Bacterial typeII secretion system protein E (IPR001482) Pfam: Clp amino ternimal domain; ATPase family associated with cellular activites no signal peptide no TMHs; High confidence in function and specificity; ATP-dependent Clp protease ATP-binding subunit clpA 1222703 clpA 4606706 clpA Azoarcus sp. BH72 ATP-dependent Clp protease ATP-binding subunit clpA YP_932636.1 1220421 R 62928 CDS YP_932637.1 119897424 4608486 complement(1222704..1223012) 1 NC_008702.1 involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation; binds to the N-terminal domain of the chaperone ClpA; ATP-dependent Clp protease adaptor protein ClpS 1223012 clpS 4608486 clpS Azoarcus sp. BH72 ATP-dependent Clp protease adaptor protein ClpS YP_932637.1 1222704 R 62928 CDS YP_932638.1 119897425 4608490 1223446..1223649 1 NC_008702.1 Cold-shock protein cspE, (When Escherichia coli is exposed to a temperature drop from 37 to 10 degrees centigrade, a 4-5 hour lag phase occurs, after which growth is resumed at a reduced rate. During the lag phase,the expression of around 13 proteins, which contain specific DNA-binding regions, is increased 2-10 fold. These so-called 'cold shock' proteins are thought to help the cell to survive in temperatures lower than optimum growth temperature.); High confidence in function and specificity; cold-shock protein 1223649 cspE 4608490 cspE Azoarcus sp. BH72 cold-shock protein YP_932638.1 1223446 D 62928 CDS YP_932639.1 119897426 4608539 complement(1223918..1224085) 1 NC_008702.1 in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif; 50S ribosomal protein L33 1224085 rpmG 4608539 rpmG Azoarcus sp. BH72 50S ribosomal protein L33 YP_932639.1 1223918 R 62928 CDS YP_932640.1 119897427 4609791 complement(1224147..1224383) 1 NC_008702.1 required for 70S ribosome assembly; 50S ribosomal protein L28 1224383 rpmB 4609791 rpmB Azoarcus sp. BH72 50S ribosomal protein L28 YP_932640.1 1224147 R 62928 CDS YP_932641.1 119897428 4609786 complement(1224488..1225165) 1 NC_008702.1 Involved in DNA double-strand break repair and recombination. Promotes the annealing of complementary single-stranded DNA and by simulation of RAD51 recombinase; DNA repair protein RadC 1225165 radC 4609786 radC Azoarcus sp. BH72 DNA repair protein RadC YP_932641.1 1224488 R 62928 CDS YP_932642.1 119897429 4609691 1225220..1226419 1 NC_008702.1 catalyzes the conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, which is then decarboxylated to form 4'-phosphopantotheine; bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase 1226419 dfp 4609691 dfp Azoarcus sp. BH72 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase YP_932642.1 1225220 D 62928 CDS YP_932643.1 119897430 4608619 1226436..1226885 1 NC_008702.1 catalyzes the formation of dUMP from dUTP; deoxyuridine 5'-triphosphate nucleotidohydrolase 1226885 dut 4608619 dut Azoarcus sp. BH72 deoxyuridine 5'-triphosphate nucleotidohydrolase YP_932643.1 1226436 D 62928 CDS YP_932644.1 119897431 4608657 1226887..1227312 1 NC_008702.1 Conserved hypothetical MutT family protein. Homology to AGR_C_3368 of Agrobacterium tumefaciens of 33% (tremble:Q7CYF2). Pfam: NUDIX domain MutT is a small bacterial protein (~12-15Kd) involved in the GO system MEDLINE:1328155 responsible for removing an oxidatively damaged form of guanine (8-hydroxy- guanine or 7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with near equal efficiency, leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate, with the concomitant release of pyrophosphate. A short conserved N-terminal region of mutT (designated the MutT domain) is also found in a variety of other prokaryotic, viral and eukaryotic proteins. No signal peptide. No TMH; Family membership; mutT family protein 1227312 4608657 azo1140 Azoarcus sp. BH72 mutT family protein YP_932644.1 1226887 D 62928 CDS YP_932645.1 119897432 4606707 complement(1227315..1228592) 1 NC_008702.1 Probable sulfide dehydrogenase, falvoprotein subunit (Flavocytochrome C flavoprotein subunit). Homology to fccB of C. vinosum of 40% (sprot|DHSU_CHRVI). Pfam: Pyridine nucleotide-disulphide oxidoreductase signal peptide no TMHs; Family membership; sulfide dehydrogenase, flavoprtein subunit 1228592 soxF 4606707 soxF Azoarcus sp. BH72 sulfide dehydrogenase, flavoprtein subunit YP_932645.1 1227315 R 62928 CDS YP_932646.1 119897433 4609883 complement(1228607..1228906) 1 NC_008702.1 Putative sulfide dehydrogenase, cytochrome subunit. Homology to fccA of C. limicola of 26% (sprot|CYSD_CHLLT(SRS)) InterPro: Cytochrome c class I (IPR003088); cytochrome c class IC (IPR008168) Pfam: Cytochrome C signal peptide no TMHs; putative sulfide dehydrogenase, cytochrome subunit 1228906 soxE 4609883 soxE Azoarcus sp. BH72 putative sulfide dehydrogenase, cytochrome subunit YP_932646.1 1228607 R 62928 CDS YP_932647.1 119897434 4609882 1229108..1229551 1 NC_008702.1 sulfur covalently-binding protein 1229551 soxY 4609882 soxY Azoarcus sp. BH72 sulfur covalently-binding protein YP_932647.1 1229108 D 62928 CDS YP_932648.1 119897435 4609885 1229562..1229873 1 NC_008702.1 Gene Function; High confidence in function and specificity; phosphodiesterase I 1229873 soxZ 4609885 soxZ Azoarcus sp. BH72 phosphodiesterase I YP_932648.1 1229562 D 62928 CDS YP_932649.1 119897436 4609886 complement(1229883..1231652) 1 NC_008702.1 catalyzes the phosphorylation/dephosphorylation of the enzyme isocitrate dehydrogenase on a specific serine which regulates activity; unphosphorylated IDH is fully active when cells are grown on glucose while the enzyme becomes phosphorylated and inactive in the presence of acetate or ethanol; bifunctional isocitrate dehydrogenase kinase/phosphatase 1231652 aceK 4609886 aceK Azoarcus sp. BH72 bifunctional isocitrate dehydrogenase kinase/phosphatase YP_932649.1 1229883 R 62928 CDS YP_932650.1 119897437 4608261 1232133..1234370 1 NC_008702.1 Isocitrate dehydrogenase [NADP]. Homology to icd2 of A. eutrophus of 80% (trembl|Q8KLU4) This monomeric type of isocitrate dehydrogenase is NADP-specific. It is an important enzyme of the TCA. Interpro: Isocitrate dehydrogenase NADP-dependent, monomeric type (IPR004436) Tigrfam: monomer-idh: isocitrate dehydrogenase NADP-dependent no singal peptide no TMHs; High confidence in function and specificity; isocitrate dehydrogenase 1234370 icd2 4608261 icd2 Azoarcus sp. BH72 isocitrate dehydrogenase YP_932650.1 1232133 D 62928 CDS YP_932651.1 119897438 4609065 1234547..1235824 1 NC_008702.1 Isocitrate dehydrogenase [NADP]. Homology to icd1 of A. eutropus of 75% (trembl|Q8KLU5) Isocitrate dehydrogenase is an important enzyme of the TCA which catalyzes the reaction: isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+ Pfam: Isocitrate/isopropylmalate dehydrogenase Tigrfam: prok_nadp_idh: isocitrate dehydrogenase no signal peptide no TMHs; High confidence in function and specificity; isocitrate dehydrogenase 1235824 icd1 4609065 icd1 Azoarcus sp. BH72 isocitrate dehydrogenase YP_932651.1 1234547 D 62928 CDS YP_932652.1 119897439 4609064 1236048..1236302 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ws0602 of W. succinogenes of 63% (trembl|Q7MSB5(SRS)) no domains predicted no singal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 1236302 4609064 azo1148 Azoarcus sp. BH72 hypothetical protein YP_932652.1 1236048 D 62928 CDS YP_932653.1 119897440 4606708 1236315..1238120 1 NC_008702.1 Conserved hypothetical sodium:solute symporter. Homology to to ws0601 of W. succinogenes of 68% (trembl|Q7M9X9). Sodium/substrate symport (or co-transport) is a widespread mechanism of solute transport across cytoplasmic membranes of pro- and eukaryotic cells. Thereby the energy stored in an inwardly directed electrochemical sodium gradient (sodium motive force, SMF) is used to drive solute accumulation against a concentration gradient. The solutes transported may be sugars, amino acids, nucleosides, inositols, vitamins,urea or anions, depending on the system. InterPro: Sodium:solute symporter family (IPR001734) Tigrfam: sss: SSS sodium solute transporter superfamily no signal peptide 12 TMHs; Family membership; putative sodium:solute symporter 1238120 4606708 azo1149 Azoarcus sp. BH72 putative sodium:solute symporter YP_932653.1 1236315 D 62928 CDS YP_932654.1 119897441 4606709 1238249..1238617 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No singal peptide 3 TMHs; hypothetical protein 1238617 4606709 azo1150 Azoarcus sp. BH72 hypothetical protein YP_932654.1 1238249 D 62928 CDS YP_932655.1 119897442 4606710 complement(1238632..1238925) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to vv11244 of V. vulnificus of 42% (trembl|Q8DCZ3(SRS)) no domains predicted no signal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 1238925 4606710 azo1151 Azoarcus sp. BH72 hypothetical protein YP_932655.1 1238632 R 62928 CDS YP_932656.1 119897443 4606711 complement(1238922..1240967) 1 NC_008702.1 EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 1240967 fusA1 4606711 fusA1 Azoarcus sp. BH72 elongation factor G YP_932656.1 1238922 R 62928 CDS YP_932657.1 119897444 4608848 complement(1241063..1242703) 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. 61%; Function unclear; major facilitator superfamily permease 1242703 4608848 azo1153 Azoarcus sp. BH72 major facilitator superfamily permease YP_932657.1 1241063 R 62928 CDS YP_932658.1 119897445 4606712 complement(1243088..1243822) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc2670 of R. solanacearum of 64% (tremble: Q8XWO5) Pfam: LrgB-like family The two products of the lrgAB operon are potential membrane proteins, and LrgA and LrgB are both thought to control of murein hydrolase activity and penicillin tolerance. no signal peptide 6 TMHs; Conserved hypothetical protein; hypothetical protein 1243822 4606712 azo1154 Azoarcus sp. BH72 hypothetical protein YP_932658.1 1243088 R 62928 CDS YP_932659.1 119897446 4606713 complement(1243819..1244181) 1 NC_008702.1 Conserved hypothetical membrane protein. This family is uncharacterised. It contains the protein LrgA that has been hypothesised to export murein hydrolases TREMBL:Q8XW06: 56% identity, 73% similarity InterPro:IPR005538; LrgA. Pfam: PF03788; LrgA; 1. ProDom: PD009239; LrgA Signal peptide present (Signal P predicted) TMH's 3 (TMHMM predicted); Conserved hypothetical protein; hypothetical protein 1244181 4606713 azo1155 Azoarcus sp. BH72 hypothetical protein YP_932659.1 1243819 R 62928 CDS YP_932660.1 119897447 4606714 complement(1244251..1245189) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03002578 of Dechloromonas aromatica of 75% (gi|41723981|ref|ZP_00150871.1|(NBCI ENTREZ)). Has PF07670, Nucleoside recognition; This region in the nucleoside transporter proteins are responsible for determining nucleoside specificity in the human CNT1 and CNT2 proteins. In the FeoB proteins, which are believed to be Fe2+ transporters, it includes the membrane pore region, so the function of this region is likely to be more general than just nucleoside specificity. This family may represent the pore and gate, with a wide potential range of specificity. Hence its name 'Gate'. no signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 1245189 4606714 azo1156 Azoarcus sp. BH72 hypothetical protein YP_932660.1 1244251 R 62928 CDS YP_932661.1 119897448 4606715 complement(1245583..1245789) 1 NC_008702.1 Hypothetical secreted protein. Signal Peptide present. No good homolgous hits in the PDB. No TMHs No domains predicted.; hypothetical protein 1245789 4606715 azo1157 Azoarcus sp. BH72 hypothetical protein YP_932661.1 1245583 R 62928 CDS YP_932662.1 119897449 4606716 1246197..1247258 1 NC_008702.1 TREMBL:Q9HWH9: 45% identity, 52% similarity 2-nitropropane dioxygenase (EC 1.13.11.32) (Nitroalkane oxidase) (2-NPD). CATALYZES THE OXYGENATIVE DENITRIFICATION OF VARIOUS ANIONIC NITROALKANES. InterPro: 2-nitropropane dioxygenase InterPro:IPR004136; 2nprop_dioxygen. IPR003009; FMN_enzyme. Pfam PF03060; NPD thiE: thiamine-phosphate pyrophosphoryl No transmembrane helices (TMHMM predicted); High confidence in function and specificity; 2-nitropropane dioxygenase 1247258 ncd2 4606716 ncd2 Azoarcus sp. BH72 2-nitropropane dioxygenase YP_932662.1 1246197 D 62928 CDS YP_932663.1 119897450 4609346 complement(1247367..1248971) 1 NC_008702.1 Catalyzes the aldol condensation of glyoxylate with acetyl-CoA to form malate as part of the second step of the glyoxylate bypass and an alternative to the tricarboxylic acid cycle; malate synthase 1248971 aceB 4609346 aceB Azoarcus sp. BH72 malate synthase YP_932663.1 1247367 R 62928 CDS YP_932664.1 119897451 4608260 complement(1248990..1249772) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to mlr4286 of M. loti of 43% (trembl|Q98EE2) InterPro: IPR002781; DUF81.This domain is found in integral membrane proteins of prokaryotes which are uncharacterized Pfam:PF01925; DUF81 Signal peptide present and presence of 6 transmembrane helices; Conserved hypothetical protein; hypothetical protein 1249772 4608260 azo1160 Azoarcus sp. BH72 hypothetical protein YP_932664.1 1248990 R 62928 CDS YP_932665.1 119897452 4606717 1249955..1250920 1 NC_008702.1 Transcriptional regulator, LysR family This protein activates the transcription of the lysA gene encoding diaminopimelate decarboxylase. LysR is also a negative regulator of its own expression. 29% 1 helixturnhelix PF03466 LysR_substrate; 1. HTH reporting nucleic acid binding motif; Specificity unclear; LysR family transcriptional regulator 1250920 4606717 azo1161 Azoarcus sp. BH72 LysR family transcriptional regulator YP_932665.1 1249955 D 62928 CDS YP_932666.1 119897453 4606718 complement(1250865..1252127) 1 NC_008702.1 Putative hydroxypyruvate reductase. Degrades an unidentified toxic product from the first step of tartrate degradation. Involved in the second step of the tartrate degradation pathway. 59% MOFRL.IPR005346; UPF0125. Pfam:PF05161; MOFRL; 1.PF03658; UPF0125; 1. Signal peptide present.; High confidence in function and specificity; hydroxypyruvate reductase 1252127 ttuD 4606718 ttuD Azoarcus sp. BH72 hydroxypyruvate reductase YP_932666.1 1250865 R 62928 CDS YP_932667.1 119897454 4610047 complement(1252153..1252941) 1 NC_008702.1 Hydroxypyruvate isomerase (EC 5.3.1.22).Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).Belongs to the hyi family. 52%; High confidence in function and specificity; putative hydroxypyruvate isomerase 1252941 gip 4610047 gip Azoarcus sp. BH72 putative hydroxypyruvate isomerase YP_932667.1 1252153 R 62928 CDS YP_932668.1 119897455 4608878 1253717..1254214 1 NC_008702.1 Conserved hypothetical protein. Homology to ORF153 of Rubrivivax gelatinosus of 48% (gi|55832787|gb|AAV66902.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1254214 4608878 azo1164 Azoarcus sp. BH72 hypothetical protein YP_932668.1 1253717 D 62928 CDS YP_932669.1 119897456 4606719 complement(1254523..1256169) 1 NC_008702.1 L-lactate permease. Homology to lctP of E. coli of 72% (sprot|LLDP_ECOLI) Transports L-lactate across the membrane. Can also transport D-lactate and glycolate. Seems to be driven by a proton motive force (By similarity). Tigrfam: lctP: L-lactate transport Pfam: L-lactate permease signal peptide 12 TMHs; High confidence in function and specificity; LctP protein 1256169 lctP 4606719 lctP Azoarcus sp. BH72 LctP protein YP_932669.1 1254523 R 62928 CDS YP_932670.1 119897457 4609124 complement(1256528..1259305) 1 NC_008702.1 Conserved hypothetical iron-sulfur binding oxidase. Homology to pa4772 of P. aeruginosa of 53% (pir|H83050) InterPro: FAD linked oxidase C-terminal (IPR004413) Pfam: FAD binding domain ; Fad linked oxidase, C-terminal domain Tigrfam: glcD: glycolate oxidase subunit GlcD no signal peptide no TMHs; Family membership; iron-sulfur binding oxidase 1259305 4609124 azo1166 Azoarcus sp. BH72 iron-sulfur binding oxidase YP_932670.1 1256528 R 62928 CDS YP_932671.1 119897458 4606720 complement(1259517..1260968) 1 NC_008702.1 Conserved hypothetical iron-sulfur protein. Homology to cv3028 of C. violaceum of 68% (trembl|Q7NTM6) Tigrfam: TIGR00273: iron-sulfur cluster binding protein Pfam: 4Fe-4S binding domain no TMHs no signal peptide; Conserved hypothetical protein; iron-sulfur protein 1260968 4606720 azo1167 Azoarcus sp. BH72 iron-sulfur protein YP_932671.1 1259517 R 62928 CDS YP_932672.1 119897459 4606721 complement(1260965..1261639) 1 NC_008702.1 Conserved hypothetical protein. Homology to DVU1781 of D.vulgaris of 45% (tremble:Q72B57). Has PF02589 DUF162 domain:Uncharacterized ACR, YkgG family. No signal peptide or TMH reported present.; hypothetical protein 1261639 4606721 azo1168 Azoarcus sp. BH72 hypothetical protein YP_932672.1 1260965 R 62928 CDS YP_932673.1 119897460 4606722 complement(1261636..1262442) 1 NC_008702.1 Conserved hypothetical protein. Homologyt to nma1648 of N. meiningitidis of 55% (trembl|Q825P5) InterPro: Domain of unknown function DUF224 (IPR004017) Pfam: Domain of unknown function no signal no TMHS; hypothetical protein 1262442 4606722 azo1169 Azoarcus sp. BH72 hypothetical protein YP_932673.1 1261636 R 62928 CDS YP_932674.1 119897461 4606723 1262710..1263492 1 NC_008702.1 Putative L-lactate dehydrogenase operon regulatory protein,; High confidence in function and specificity; L-lactate dehydrogenase operon regulatory protein 1263492 lldR 4606723 lldR Azoarcus sp. BH72 L-lactate dehydrogenase operon regulatory protein YP_932674.1 1262710 D 62928 CDS YP_932675.1 119897462 4609166 1264770..1265240 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. signal peptide. no TMH; hypothetical protein 1265240 4609166 azo1171 Azoarcus sp. BH72 hypothetical protein YP_932675.1 1264770 D 62928 CDS YP_932676.1 119897463 4606724 1265498..1265653 1 NC_008702.1 Conserved hypothetical protein. Homology to the N-terminus of NE0717 of Nitrosomonas europaea of 56% (tremble:Q82WG3). Pfam: Cold shock protein domain RNA-binding domain that functions as a RNA-chaperone in bacteria and is involved in regulating translation in eukaryotes. Contains sub-family of RNA-binding domains in the Rho transcription termination factor. No signal peptide. No TMHs; hypothetical protein 1265653 4606724 azo1172 Azoarcus sp. BH72 hypothetical protein YP_932676.1 1265498 D 62928 CDS YP_932677.1 119897464 4606725 1265650..1266129 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to the C-terminus of ne0717 of N. europaea of 57% (trembl|Q82WG3). Pfam: Protein of unknown function (DUF1294). No signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 1266129 4606725 azo1173 Azoarcus sp. BH72 hypothetical protein YP_932677.1 1265650 D 62928 CDS YP_932678.1 119897465 4606726 1266287..1266538 1 NC_008702.1 Hypothetical protein. No homology to the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 1266538 4606726 azo1174 Azoarcus sp. BH72 hypothetical protein YP_932678.1 1266287 D 62928 CDS YP_932679.1 119897466 4606727 1266596..1269127 1 NC_008702.1 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4). 76% EGF_like.IPR001327; FAD_pyr_redox. IPR007419;Fer2_BFD.IPR006066; Nir_Si.IPR006067; Nir_Sir_4Fe4S. IPR005117; NiR_SiR_beta_fer. IPR001100; Pyr_redox. Pfam: PF04324; Fer2_BFD; 1.PF01077; NIR_SIR; 1.PF03460; NIR_SIR_ferr; 1. PF00070; Pyr_redox; 1. In E.coli, the NADH-dependent nitrite reductase catalyzes the six-electron reduction of nitrite to ammonia and also catalyzes the two-electron reduction of hydroxylamine to ammonia. The enzyme is a FAD-flavoprotein, and also contains a siroheme and one 2Fe-2S center.; High confidence in function and specificity; nitrite reductase (NAD(P)H) large subunit 1269127 nirB 4606727 nirB Azoarcus sp. BH72 nitrite reductase (NAD(P)H) large subunit YP_932679.1 1266596 D 62928 CDS YP_932680.1 119897467 4609380 1269149..1269487 1 NC_008702.1 Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4). REQUIRED FOR ACTIVITY OF THE REDUCTASE. 66% simialr to the nirD protein from R. solanacearum,a probable nitrite reductase NADPH (small subunit) oxidoreductase [EC:1.7.1.4]. TREMBL:Q8XQK3. InterPro: Rieske iron-sulfur protein 2Fe-2S subunit In E.coli, the NADH-dependent nitrite reductase catalyzes the six-electron reduction of nitrite to ammonia and also catalyzes the two-electron reduction of hydroxylamine to ammonia. The enzyme is a FAD-flavoprotein, and also contains a siroheme and one 2Fe-2S center.; Conserved hypothetical protein; nitrite reductase small subunit 1269487 nirD 4609380 nirD Azoarcus sp. BH72 nitrite reductase small subunit YP_932680.1 1269149 D 62928 CDS YP_932681.1 119897468 4609382 1269519..1270331 1 NC_008702.1 76% For/Nit_transpt. Pfam: PF01226; Form_Nir_trans; 1. In E. coli, NirC is a putative nitrite transporter which is a member of the FNT family of formate and nitrite transporters. The nirC gene is located in the nir operon which codes for a NADH-dependent nitrite reductase. NirC may function to import nitrite as a substrate for this enzyme complex. The nir operon is anaerobically expressed and is repressed by oxygen. TMhelix:6; High confidence in function and specificity; putative nitrite transporter 1270331 nirC 4609382 nirC Azoarcus sp. BH72 putative nitrite transporter YP_932681.1 1269519 D 62928 CDS YP_932682.1 119897469 4609381 1270372..1272093 1 NC_008702.1 Putative serin/threonin protein kinase,; Family membership; putative serine/threonine protein kinase 1272093 4609381 azo1178 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_932682.1 1270372 D 62928 CDS YP_932683.1 119897470 4606728 complement(1272191..1272715) 1 NC_008702.1 High confidence in function and specificity; ureidoglycolate hydrolase 1272715 allA 4606728 allA Azoarcus sp. BH72 ureidoglycolate hydrolase YP_932683.1 1272191 R 62928 CDS YP_932684.1 119897471 4608304 complement(1272715..1273755) 1 NC_008702.1 catalyzes the formation of S-ureidoglycolate and urea from allantoate; allantoicase 1273755 alc 4608304 alc Azoarcus sp. BH72 allantoicase YP_932684.1 1272715 R 62928 CDS YP_932685.1 119897472 4608292 complement(1274116..1275063) 1 NC_008702.1 Polysaccharide deacetylase family protein.This family of polysaccharide deacetylases includes NodB(nodulation protein B from Rhizobium) which is a chitooligosaccharide deacetylase.It also includes chitin deacetylase from yeast,and endoxylanases which hydrolyses glucosidic bonds in xylan. 62% Polysac_deacet. Pfam:PF01522; Polysacc_deac_1. TIGR:PP4286.; Function unclear; deacetylase 1275063 4608292 azo1181 Azoarcus sp. BH72 deacetylase YP_932685.1 1274116 R 62928 CDS YP_932686.1 119897473 4606729 complement(1275084..1275611) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS01483 of R.solanacearum of 55% (tremble:Q8XXJ2). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1275611 4606729 azo1182 Azoarcus sp. BH72 hypothetical protein YP_932686.1 1275084 R 62928 CDS YP_932687.1 119897474 4606730 complement(1275648..1276844) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS01489 of Ralstonia solanacearum of 50% (trembl|Q8XXJ8(SRS)) Has PF06181:Protein of unknown function (DUF989);This family consists of several hypothetical bacterial proteins of unknown function. no signal peptide 8 TMHs; Conserved hypothetical protein; hypothetical protein 1276844 4606730 azo1183 Azoarcus sp. BH72 hypothetical protein YP_932687.1 1275648 R 62928 CDS YP_932688.1 119897475 4606731 complement(1276863..1277213) 1 NC_008702.1 Conserved hypothetical transthyretin. Homology to pa1518 of P. aeruniosa of 62% (sprot|YF18_PSEAE) InterPro: Transthyretin precursor (IPR000895) Pfam: Transthyretin precursor no signal peptide no TMHs; Conserved hypothetical protein; putative transthyretin 1277213 4606731 azo1184 Azoarcus sp. BH72 putative transthyretin YP_932688.1 1276863 R 62928 CDS YP_932689.1 119897476 4606732 complement(1277327..1278304) 1 NC_008702.1 TREMBL:Q8PC98: 42% identity, 54% similarity. Proline iminopeptidase (EC 3.4.11.5) (Prolyl aminopeptidase) (PAP). SPECIFICALLY CATALYZES THE REMOVAL OF N-TERMINAL PROLINE RESIDUES FROM PEPTIDES (BY SIMILARITY). InterPro:IPR000073; A/b_hydrolase. IPR003089; AB_hydrolase. IPR002410; Peptidase_S33. IPR005944; Pept_S33. IPR000379; Ser_estrs. Pfam PF00561; Abhydrolase_1; TIGRFAMs: TIGR01249; pro_imino_pep_1 TIGR00149: conserved hypothetical protein; Specificity unclear; proline iminopeptidase 1278304 pip 4606732 pip Azoarcus sp. BH72 proline iminopeptidase YP_932689.1 1277327 R 62928 CDS YP_932690.1 119897477 4609582 1278781..1279233 1 NC_008702.1 hypothetical protein 1279233 4609582 azo1186 Azoarcus sp. BH72 hypothetical protein YP_932690.1 1278781 D 62928 CDS YP_932691.1 119897478 4606733 1279714..1280628 1 NC_008702.1 possibly involved in transport of pyrroloquinoline quinone transport; pyrroloquinoline quinone biosynthesis protein PqqB 1280628 pqqB 4606733 pqqB Azoarcus sp. BH72 pyrroloquinoline quinone biosynthesis protein PqqB YP_932691.1 1279714 D 62928 CDS YP_932692.1 119897479 4609618 1280664..1281455 1 NC_008702.1 Required in the synthesis of PPQ, but its exact function is unknown; pyrroloquinoline quinone biosynthesis protein PqqC 1281455 pqqC 4609618 pqqC Azoarcus sp. BH72 pyrroloquinoline quinone biosynthesis protein PqqC YP_932692.1 1280664 D 62928 CDS YP_932693.1 119897480 4609619 1281634..1281900 1 NC_008702.1 Coenzyme PQQ synthesis protein D .This family contains several bacterial coenzyme PQQ synthesis protein D (PqqD) sequences. This protein is required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis. 65% similarity with Klebsiella pneumoniae and 80%; Family membership; PqqD protein 1281900 pqqD 4609619 pqqD Azoarcus sp. BH72 PqqD protein YP_932693.1 1281634 D 62928 CDS YP_932694.1 119897481 4609620 1281902..1283056 1 NC_008702.1 Coenzyme PQQ synthesis protein E (Pyrroloquinoline quinone biosynthesis protein E).PART OF THE PQQ OPERON. Q88QV8: 58% identity, 74% similarity with Klebsiella pneumoniae InterProIPR000385, InterProIPR007197,PfamPF04055, SMARTSM00729, InterProIPR006638 cofactor:iron-sulfur cluster (potential). pathway:pyrroloquinoline quinone (pqq) biosynthesis. similarity: belongs to the radical sam superfamily, pqqE family TIGR PP0376; HAMAP MF_00660; Pfam PF04055; Radical_SAM; PROSITE: PS01305; MOAA_NIFB_PQQE; High confidence in function and specificity; pyrroloquinoline quinone biosynthesis protein PqqE 1283056 pqqE 4609620 pqqE Azoarcus sp. BH72 pyrroloquinoline quinone biosynthesis protein PqqE YP_932694.1 1281902 D 62928 CDS YP_932695.1 119897482 4610020 complement(1283387..1283563) 1 NC_008702.1 Hypothetical protein. Has very bad homology with hits in the database. No Signficant motifs, domains or Signal peptide known to be present.; hypothetical protein 1283563 4610020 azo1191 Azoarcus sp. BH72 hypothetical protein YP_932695.1 1283387 R 62928 CDS YP_932696.1 119897483 4606734 complement(1283668..1284468) 1 NC_008702.1 Conserved hypothetical protein. Homology to XAC0112 of X.axonopodis of 44% (trembl:Q8PR54). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1284468 4606734 azo1192 Azoarcus sp. BH72 hypothetical protein YP_932696.1 1283668 R 62928 CDS YP_932697.1 119897484 4606735 complement(1284477..1285598) 1 NC_008702.1 Probable 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (4HPPD) (HPD) (HPPDase). Homology to hppD of Pseudomonas sp. of 56% (pir|S21209) Catalysis of the reaction: 4-hydroxyphenylpyruvate + O2 = homogentisate + CO2. Pfam: Glaxolase/Bleomycin resistance protein no signal peptide no TMHs; Family membership; 4-hydroxyphenylpyruvate dioxygenase 1285598 hppD 4606735 hppD Azoarcus sp. BH72 4-hydroxyphenylpyruvate dioxygenase YP_932697.1 1284477 R 62928 CDS YP_932698.1 119897485 4609025 1286022..1287257 1 NC_008702.1 Permease member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. 2A0121: H+ Antiporter protein 24% similarity to NorA who confer relatively high resistance to hydrophilic quinolones such as norfloxacin, enoxacin, ofloxacin, and ciprofloxacin in S. aureus. 20% similarity to TapA a multidrug efflux pump wich confers low level of resistance to aminoglycosides and tetracycline in Mycobaterium fortuitum and M. tuberculosis.; Specificity unclear; putative integral membrane efflux protein 1287257 4609025 azo1194 Azoarcus sp. BH72 putative integral membrane efflux protein YP_932698.1 1286022 D 62928 CDS YP_932699.1 119897486 4606736 1287319..1288497 1 NC_008702.1 DapATase; functions in arginine biosynthetic pathway; catalyzes the formation of N-acetyl-L-glutamate 5-semialdehyde from 2-oxoglutarate and N(2)-acetyl-L-ornithine; acetylornithine transaminase 1288497 argD 4606736 argD Azoarcus sp. BH72 acetylornithine transaminase YP_932699.1 1287319 D 62928 CDS YP_932700.1 119897487 4608340 complement(1288769..1289806) 1 NC_008702.1 Conserved hypothetical protein. Homology to ypo3484 of y. pestis of 60% (trembl|Q8ZBD2). Pfam: Luciferase-like monooxygenase. no signla peptide no TMHs; hypothetical protein 1289806 4608340 azo1196 Azoarcus sp. BH72 hypothetical protein YP_932700.1 1288769 R 62928 CDS YP_932701.1 119897488 4606737 complement(1289811..1290620) 1 NC_008702.1 similar protein in Methanocaldococcus converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate as the first step in methanopterin biosynthesis; putative GTP cyclohydrolase 1290620 4606737 azo1197 Azoarcus sp. BH72 putative GTP cyclohydrolase YP_932701.1 1289811 R 62928 CDS YP_932702.1 119897489 4606738 complement(1290707..1292566) 1 NC_008702.1 catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate; 1-deoxy-D-xylulose-5-phosphate synthase 1292566 dxs 4606738 dxs Azoarcus sp. BH72 1-deoxy-D-xylulose-5-phosphate synthase YP_932702.1 1290707 R 62928 CDS YP_932703.1 119897490 4608659 complement(1292594..1293496) 1 NC_008702.1 Geranylgeranyl pyrophosphate synthetase chloroplast precursor (GGPP synthetase) (GGPS) [Includes: Dimethylallyltransferase (EC 2.5.1.1); Geranyltranstransferase (EC 2.5.1.10); Farnesyltranstransferase (EC 2.5.1.29)]. catalyzes the trans-addition of the three molecules of ipp onto dmapp to form geranylgeranyl pyrophosphate. InterPro: Polyprenyl synthetase; High confidence in function and specificity; geranyltranstransferase 1293496 ispA 4608659 ispA Azoarcus sp. BH72 geranyltranstransferase YP_932703.1 1292594 R 62928 CDS YP_932704.1 119897491 4609094 complement(1293493..1293747) 1 NC_008702.1 Probable Probable exodeoxyribonuclease VII small subunit . InterPro: Exonuclease VII small subunit.; Function unclear; exodeoxyribonuclease VII 1293747 xseB 4609094 xseB Azoarcus sp. BH72 exodeoxyribonuclease VII YP_932704.1 1293493 R 62928 CDS YP_932705.1 119897492 4610130 1293877..1294977 1 NC_008702.1 AlcE: iron-sulfur-containing dioxygenases, probably involved in alcaligin biosynthesis pathway in Bordetella species. Putative iron-sulfure protein involved in aromatic compounds degradations. Choline monooxygenase chloroplast precursor (EC 1.14.15.7). Catalyzes the first step of the osmoprotectant glycine betaine synthesis. InterPro: Rieske iron-sulfur protein 2Fe-2S subunit hypA: hydrogenase expression/formation pr; Function unclear; putative iron-sulfur protein 1294977 alcE 4610130 alcE Azoarcus sp. BH72 putative iron-sulfur protein YP_932705.1 1293877 D 62928 CDS YP_932706.1 119897493 4608293 complement(1295352..1296290) 1 NC_008702.1 catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1296290 ispH 4608293 ispH Azoarcus sp. BH72 4-hydroxy-3-methylbut-2-enyl diphosphate reductase YP_932706.1 1295352 R 62928 CDS YP_932707.1 119897494 4609189 complement(1296320..1296748) 1 NC_008702.1 Putative peptidyl-prolyl cis-trans isomerase. Homology to slpA of E. coli of 39% (sprot|FKBX_ECOLI) Accelerate protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. InterPro: FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase)(IPR001179) Pfam: FKBP-type peptidyl-prolyl cis-trans isomerase no signal peptide no TMHs; Family membership; putative peptidyl-prolyl cis-trans isomerase 1296748 slpA 4609189 slpA Azoarcus sp. BH72 putative peptidyl-prolyl cis-trans isomerase YP_932707.1 1296320 R 62928 CDS YP_932708.1 119897495 4609872 complement(1296745..1297275) 1 NC_008702.1 lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis; lipoprotein signal peptidase 1297275 lspA 4609872 lspA Azoarcus sp. BH72 lipoprotein signal peptidase YP_932708.1 1296745 R 62928 CDS YP_932709.1 119897496 4609182 complement(1297268..1300066) 1 NC_008702.1 IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme; isoleucyl-tRNA synthetase 1300066 ileS 4609182 ileS Azoarcus sp. BH72 isoleucyl-tRNA synthetase YP_932709.1 1297268 R 62928 CDS YP_932710.1 119897497 4606739 complement(1300081..1301079) 1 NC_008702.1 Riboflavin biosynthesis protein ribF [Includes: Riboflavin kinase (EC 2.7.1.26) (Flavokinase); FMN adenylyltransferase (EC 2.7.7.2) (FAD pyrophosphorylase) (FAD synthetase)]. ribF: riboflavin biosynthesis protein R; High confidence in function and specificity; riboflavin kinase/FMN adenylyltransferase 1301079 ribF 4606739 ribF Azoarcus sp. BH72 riboflavin kinase/FMN adenylyltransferase YP_932710.1 1300081 R 62928 CDS YP_932711.1 119897498 4609724 1301459..1301782 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 1301782 4609724 azo1207 Azoarcus sp. BH72 hypothetical protein YP_932711.1 1301459 D 62928 CDS YP_932712.1 119897499 4606740 1301779..1302555 1 NC_008702.1 Hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Glyoxalase II) (Glx II). Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Zn dependent Hydrolase belonging to glyoxalase II family of proteins. Presence of KOW motif,and PA domain.Presence of signal peptide. probably functioning as Carbamoylphosphate synthase L chain. 50% identity and 60% similarity to Bordetella pertussis hydrolase. InterPro: Metallo-beta-lactamase superfamily gidA: glucose-inhibited division protein; Family membership; hydroxyacylglutathione hydrolase 1302555 gloB1 4606740 gloB1 Azoarcus sp. BH72 hydroxyacylglutathione hydrolase YP_932712.1 1301779 D 62928 CDS YP_932713.1 119897500 4608909 1302969..1303301 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to the data bank. No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1303301 4608909 azo1209 Azoarcus sp. BH72 hypothetical protein YP_932713.1 1302969 D 62928 CDS YP_932714.1 119897501 4606741 1303298..1304290 1 NC_008702.1 TREMBLnew:CAE26305: 46% identity, 65% similarity. sprot:YJIA_ECOLI, 33% identity, 55% similarity Hypothetical protein. Pfam:cobW: Cobalmine synthesis protein; ABC_tran TIGRFAM: MMR_HSR1- GTPASE of unknown function InterPro: Cobalamin synthesis protein/P47K mobB: molybdopterin-guanine dinucleotid; Conserved hypothetical protein; putative GTPase 1304290 4606741 azo1210 Azoarcus sp. BH72 putative GTPase YP_932714.1 1303298 D 62928 CDS YP_932715.1 119897502 4606742 1304287..1305360 1 NC_008702.1 Conserved hypothetical protein. Homology bll7767 of B. japonicum of 30% (trembl|Q89CN0) Pfam: WD domain, G-beta repeat no signal peptide no TMHs; hypothetical protein 1305360 4606742 azo1211 Azoarcus sp. BH72 hypothetical protein YP_932715.1 1304287 D 62928 CDS YP_932716.1 119897503 4606743 1305397..1305612 1 NC_008702.1 Conserved hypothetical protein. Homology to Rsph03000178 of Rhodobacter sphaeroides of 37%. No domains predicted. No signal peptide. No TMHs; hypothetical protein 1305612 4606743 azo1212 Azoarcus sp. BH72 hypothetical protein YP_932716.1 1305397 D 62928 CDS YP_932717.1 119897504 4606744 1305750..1306688 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to qbdB of Pseudomonas putida of 50% (gi|22779360|dbj|BAC15558.1|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 1306688 qbdB1 4606744 qbdB1 Azoarcus sp. BH72 hypothetical protein YP_932717.1 1305750 D 62928 CDS YP_932718.1 119897505 4609677 1306817..1307347 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Rgel02003151 of Rubrivivax gelatinosus of 37% (gi|47572524|ref|ZP_00242567.1|(NBCI ENTREZ)). PF06496,Protein of unknown function (DUF1097);IPR009476;This family consists of several bacterial putative membrane proteins. signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 1307347 4609677 azo1214 Azoarcus sp. BH72 hypothetical protein YP_932718.1 1306817 D 62928 CDS YP_932719.1 119897506 4606745 1307593..1308540 1 NC_008702.1 Part of the ABC transporter complex mntABC involved in manganese uptake. 28% Similar to the putative periplasmic-binding protein MntC precursor in N gonorrhoaeae, also involved in the resistance to oxidative stress. TREMBL:Q9F4F6 InterPro:IPR006128; Lipoprotein_4.IPR006127; SBP_bac_9. Pfam:PF01297; SBP_bac_9; 1. Signal peptide present.TMHelix:1 This operon probably is also involved in other cations uptake like Fe,Cu and Zn.This protein also could act as an adhesin which is involved on adherence to extracellular matrix.; High confidence in function and specificity; putative periplasmic solute binding protein 1308540 mntC2 4606745 mntC2 Azoarcus sp. BH72 putative periplasmic solute binding protein YP_932719.1 1307593 D 62928 CDS YP_932720.1 119897507 4609240 1308560..1309357 1 NC_008702.1 Manganese transport system ATP-binding protein mntA. This protein is probably a component of a manganese permease a binding protein-dependent ATP-driven transport system (mntABC). Probably responsible for energy coupling to the transport system. 40% AAA_ATPase.IPR003439,AAA ATPase superfamily; ABC_transporter. Pfam: PF00005; ABC_tran; 1. This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system ATP-binding protein 1309357 mntA2 4609240 mntA2 Azoarcus sp. BH72 putative manganese transport system ATP-binding protein YP_932720.1 1308560 D 62928 CDS YP_932721.1 119897508 4609235 1309369..1310265 1 NC_008702.1 Part of the ABC transporter complex mntABC involved in manganese import. Probably responsible for the translocation of the substrate across the membrane. 28% IPR001626; ABC_transpt3. Pfam; PF00950; ABC-3; 1. TmHelix:8 This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system permease 1310265 mntB2 4609235 mntB2 Azoarcus sp. BH72 putative manganese transport system permease YP_932721.1 1309369 D 62928 CDS YP_932722.1 119897509 4609237 1310262..1311221 1 NC_008702.1 Part of the ABC transporter complex mntABC involved in manganese import. Probably responsible for the translocation of the substrate across the membrane. Similar to the permease, MntB in Synechocystis 6803. 30% IPR001626; ABC_transpt3. Pfam; PF00950; ABC-3; 1. This operon probably is also involved in other cations uptake like Fe,Cu and Zn.; High confidence in function and specificity; putative manganese transport system permease 1311221 mntB3 4609237 mntB3 Azoarcus sp. BH72 putative manganese transport system permease YP_932722.1 1310262 D 62928 CDS YP_932723.1 119897510 4609238 1311744..1313414 1 NC_008702.1 Conserved hypothetical aromatic/alkene monooxygenase, subunit alpha Homology to blr3677 of B. japonicum (trembl|Q89P06(SRS) Bacterial aromatic/alkene monooxygenase is a multicomponent enzyme that catabolises phenol and some of its methylated derivatives (like methane and toulene). InterPro:IPR003430 Pfam: Methane/Phenol/Toluene hydroxylase (PF02332) no signal peptide no TMHs; Specificity unclear; aromatic/alkene monooxygenase subunit alpha 1313414 4609238 azo1219 Azoarcus sp. BH72 aromatic/alkene monooxygenase subunit alpha YP_932723.1 1311744 D 62928 CDS YP_932724.1 119897511 4606746 1313496..1314539 1 NC_008702.1 Conserved hypothetical aromatic/alkene monooxygenase, subunit gamma. Homology to blr3678 of B. japonicum of 60% (trembl|Q89P05(SRS) Bacterial aromatic/alkene monooxygenase is a multicomponent enzyme that catabolises phenol and some of its methylated derivatives (like methane and toulene). Interpro: Phenol hydroxylase reductase family (IPR001221); Oxidoredutase FAD and NAD(P)-binding domain (IPR000134); NADH:cytochrome b5 reductase (CBR) (IPR000134) Pfam: 2Fe-2S iron-sulfur cluster binding domain; oxidoreductase FAD-binding domain; Oxidoreductase NAD-binding domain no signal peptide no TMHs; Specificity unclear; aromatic/alkene monooxygenase subunit gamma 1314539 4606746 azo1220 Azoarcus sp. BH72 aromatic/alkene monooxygenase subunit gamma YP_932724.1 1313496 D 62928 CDS YP_932725.1 119897512 4606747 1314604..1315695 1 NC_008702.1 Conserved hypothetical aromatic/alkene monooxygenase, subunit beta Homology to blr3679 of B. japonicum of 53% (trembl|Q89P04(SRS) Bacterial aromatic/alkene monooxygenase is a multicomponent enzyme that catabolises phenol and some of its methylated derivatives (like methane and toulene). Interpro: Methane/Penol/Toluene hydroxylase (IPR003430) Pfam: Methane/Penol/Toluene hydroxylase (PF02332) no signal peptide no TMHs; Specificity unclear; aromatic/alkene monooxygenase subunit beta 1315695 4606747 azo1221 Azoarcus sp. BH72 aromatic/alkene monooxygenase subunit beta YP_932725.1 1314604 D 62928 CDS YP_932726.1 119897513 4606748 1315744..1316100 1 NC_008702.1 Phenol hydroxylase P2 protein (Phenol 2-monooxygenase P2 component)68% similarity to SWISSPROT:P19731,Pseudomonas sp DmpM or Phenol hydroxylase component P2, this protein lacks redox co-factors and is required for optimal turnover of Phenol hydroxylase . Phenol hydroxylase catabolises phenol and some of its methylated derivatives in the first step of phenol biodegradation, and is required for growth on phenol. The multicomponent enzyme is made up of P0, P1, P2, P3, P4 and P5 polypeptides. Pfam:PF02406, InterPro:IPR003454 91% similarity with hypothetical protein Rgel01002820 [Rubrivivax gelatinosus PM1] NO Signal Peptide No TMH's. MmoB/DmpM family; High confidence in function and specificity; phenol 2-monooxygenase 1316100 dmpM 4606748 dmpM Azoarcus sp. BH72 phenol 2-monooxygenase YP_932726.1 1315744 D 62928 CDS YP_932727.1 119897514 4608629 1316323..1317960 1 NC_008702.1 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is not essential for growth; chaperonin GroEL 1317960 groEL 4608629 groEL Azoarcus sp. BH72 chaperonin GroEL YP_932727.1 1316323 D 62928 CDS YP_932728.1 119897515 4608938 1318051..1318197 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 1318197 4608938 azo1224 Azoarcus sp. BH72 hypothetical protein YP_932728.1 1318051 D 62928 CDS YP_932729.1 119897516 4606749 1318385..1320445 1 NC_008702.1 Some bacterial regulatory proteins activate the expression of genes from promoters recognized by core RNA polymerase associated with the alternative sigma-54 factor. These have a conserved domain of about 230 residues involved in the ATP-dependent interaction with sigma-54, TREMBL:Q8RM05 (41% identity); TREMBL:Q880V5 (32% identity). Pfam (PF02954): Bacterial regulatory protein,Fis family. Pfam (PF00158): Sigma-54 interaction domain. HTH reporting nucleic acid binding motif.; Family membership; sigma-54 dependent transcriptional regulator 1320445 4606749 azo1225 Azoarcus sp. BH72 sigma-54 dependent transcriptional regulator YP_932729.1 1318385 D 62928 CDS YP_932730.1 119897517 4606750 1320918..1321319 1 NC_008702.1 Glyoxalase I catalyzes the first step of the glyoxal pathway. S-lactoylglutathione is then converted by glyoxalase II to lactic acid. Similar to trembl|Q8YEJ0 (61%), to trembl|Q8DB12 (58%) and to trembl|Q8UCM7 (52%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase; Specificity unclear; lactoylglutathione lyase 1321319 lguL 4606750 lguL Azoarcus sp. BH72 lactoylglutathione lyase YP_932730.1 1320918 D 62928 CDS YP_932731.1 119897518 4609136 1321373..1321966 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to blr7490 of B. japonicum of 31% (trembl|Q89DE9(SRS)). No domains predicted. No TMHs Signal peptide present; Conserved hypothetical protein; hypothetical protein 1321966 4609136 azo1227 Azoarcus sp. BH72 hypothetical protein YP_932731.1 1321373 D 62928 CDS YP_932732.1 119897519 4606751 1321992..1323557 1 NC_008702.1 Putative glucose dehydrogenase alpha subunit. Homology to gdhAlpha of B. cepacia of 29% (trembl|Q8GQE7) InterPro: NAD binding site (IPR000205) Pfam: GMC oxidoreductase no signal peptide no TMHs; Function unclear; putative glucose dehydrogenase subunit alpha 1323557 gdhAlpha1 4606751 gdhAlpha1 Azoarcus sp. BH72 putative glucose dehydrogenase subunit alpha YP_932732.1 1321992 D 62928 CDS YP_932733.1 119897520 4608873 1323688..1324407 1 NC_008702.1 Hypothetical secreted protein. No good homology with hits in the database. Signal peptide present. No TMHs. No domains predicted; hypothetical protein 1324407 4608873 azo1229 Azoarcus sp. BH72 hypothetical protein YP_932733.1 1323688 D 62928 CDS YP_932734.1 119897521 4606752 complement(1324442..1324726) 1 NC_008702.1 Conserved hypothetical membrane protein, 40% identity (49% Similarity) to TrEMBL;Q6D7F7. Signa P reporting Signal peptide present. TMHMM2 reporting 2 TMH's present.; Conserved hypothetical protein; hypothetical protein 1324726 4606752 azo1230 Azoarcus sp. BH72 hypothetical protein YP_932734.1 1324442 R 62928 CDS YP_932735.1 119897522 4606753 complement(1324874..1326016) 1 NC_008702.1 Cytochrome D ubiquinol oxidase subunit II (EC 1.10.3.-) (Cytochrome BD-I oxidase subunit II). Cytochrome bd type terminal oxidases catalyse quinol dependent, Na+ independent oxygen uptake. Members of this family are integral membrane proteins and contain a protoheame IX center B558. It may play an important role in microaerobic nitrogen fixation. Tigrfam: cydB: cytochrome d ubiquinol oxidase subunit II Pfam: cytochrome oxidase subunit II no signal peptide 8 TMHs; High confidence in function and specificity; cytochrome bd type terminal oxidase subunit II 1326016 cydB 4606753 cydB Azoarcus sp. BH72 cytochrome bd type terminal oxidase subunit II YP_932735.1 1324874 R 62928 CDS YP_932736.1 119897523 4608553 complement(1326028..1327596) 1 NC_008702.1 Probable cytochrome bd type terminal oxidase,subunit I. Homology to cydA of A. vinelandii of 67% (sprot|CYDA_AZOVI). Cytochrome bd type terminal oxidases catalyse quinol dependent, Na+ independent oxygen uptake. Members of this family are integral membrane proteins and contain a protoheame IX center B558. It may play an important role in microaerobic nitrogen fixation. InterPro: Cytochrome bd ubiquinol oxidase subunit I (IPR002585) Pfam: Bacterial cytochrome ubiquinol oxidase no singal peptide 9 TMHs; High confidence in function and specificity; cytochrome bd type terminal oxidase subunit I 1327596 cydA 4608553 cydA Azoarcus sp. BH72 cytochrome bd type terminal oxidase subunit I YP_932736.1 1326028 R 62928 CDS YP_932737.1 119897524 4608552 complement(1327586..1327813) 1 NC_008702.1 Hypothetical membrane protein. no homology to to the data bank. no domains predicted. no signal peptide. 1 TMHs; hypothetical protein 1327813 4608552 azo1233 Azoarcus sp. BH72 hypothetical protein YP_932737.1 1327586 R 62928 CDS YP_932738.1 119897525 4606754 1327954..1329348 1 NC_008702.1 Probable ranscriptional regulator,; Specificity unclear; transcriptional regulator 1329348 4606754 azo1234 Azoarcus sp. BH72 transcriptional regulator YP_932738.1 1327954 D 62928 CDS YP_932739.1 119897526 4606755 1329607..1330194 1 NC_008702.1 Ubiquinol-cytochrome C reductase iron-sulfur protein. Homology to petA of R. gelantinosus of 60% (trembl|Q93SY7). Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Pfam: Riedke [2Fe-2S] domain no TMHs signal peptide; High confidence in function and specificity; ubiquinol-cytochrome C reductase iron-sulfur protein 1330194 petA2 4606755 petA2 Azoarcus sp. BH72 ubiquinol-cytochrome C reductase iron-sulfur protein YP_932739.1 1329607 D 62928 CDS YP_932740.1 119897527 4609525 1330265..1332022 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs. Similar to TREMBL:Q88H94 (52% identity); TREMBL:Q9RXZ1 (29% identity); SWISSPROT:Q9WYC4 (25% identity). Pfam (PF00664): ABC transporter transmembrane region. Pfam (PF00005): ABC transporter. TMHMM reporting six transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; ABC transporter permease and ATP-binding protein 1332022 4609525 azo1236 Azoarcus sp. BH72 ABC transporter permease and ATP-binding protein YP_932740.1 1330265 D 62928 CDS YP_932741.1 119897528 4606756 1332019..1332723 1 NC_008702.1 Subtilisin Savinase precursor, 36% identity to SwissProt; P29600. Has PF00082, Subtilase family;IPR000209, Pept_S8_S53;Subtilases are a family of serine proteases. They appear to have independently and convergently evolved an Asp/Ser/His catalytic triad, like that found in the trypsin serine proteases (see Trypsin). Structure is an alpha/beta fold containing a 7-stranded parallel beta sheet, order 2314567. PROSITE;) PS00136; SUBTILASE_ASP; 1. PS00137; SUBTILASE_HIS; 1. PS00138; SUBTILASE_SER; 1.; subtilisin 1332723 subC 4606756 subC Azoarcus sp. BH72 subtilisin YP_932741.1 1332019 D 62928 CDS YP_932742.1 119897529 4609903 1332713..1334038 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA2217 of Azoarcus sp. EbN1 of 32% (gnl|keqq|eba:ebA2217(KEGG)). C-terminus is not homolog to COG0644. signal peptide present. no TMHS.; Conserved hypothetical protein; hypothetical protein 1334038 4609903 azo1238 Azoarcus sp. BH72 hypothetical protein YP_932742.1 1332713 D 62928 CDS YP_932743.1 119897530 4606757 1334490..1336076 1 NC_008702.1 Probable quinohemoprotein amine dehydrogenase,alpha subunit. Homology to qhpA of P.putida of 50% (gi|18655859|pdb|1JMZ|A(PDB (RCSB))). Quinoproteins are a class of amine-oxidising enzymes that catalyse the oxidation of biological amines, using quinone as a redox cofactor to store reducing equivalents. Quinohemoprotein amine dehydrogenase (QH-AmDH) from bacteria represents a new class of quinoproteins that contains both quinone and one or two hemes as redox active groups. The presence of extra redox active groups allows for intramolecular electron transfer. QH-AmDH is a heterotrimeric enzyme containing alpha, beta and gamma chains encoded by separate genes. The alpha chain makes contact with both the beta and gamma chains. The small gamma chain forms three intra-chain cross-links via thioester bonds between cysteine and aspartic or glutamic acid residues, thereby encaging the cysteine tryptophylquinone cofactor. The largest chain, alpha, contains two heme c groups. The alpha chain is folded into four domains, domain 1 forming a diheme cytochrome. Domains 2, 3 and 4 are antiparallel beta-barrel structures. Has PF00034, Cytochromes c (cytC). Interpro (IPR009111) Quinohemoprotein amine dehydrogenase, alpha chain, domain 3. Signal peptide present. No TMHs.; High confidence in function and specificity; quinohemoprotein amine dehydrogenase subunit alpha 1336076 qhpA 4606757 qhpA Azoarcus sp. BH72 quinohemoprotein amine dehydrogenase subunit alpha YP_932743.1 1334490 D 62928 CDS YP_932744.1 119897531 4609679 1336144..1337574 1 NC_008702.1 Conserved hypothetical qinohemoprotein amine dehydrogenase, unknown subunit. Homology to P. putida of 71% (gi|16950513|dbj|BAB72009.1|(NBCI ENTREZ)). InterPro:IPR000385; MoaA_NifB_PqqE. IPR007197; Radical_SAM. Pfam:PF04055; Radical_SAM. No signal peptide. No TMHs; Family membership; putative qinohemoprotein amine dehydrogenase 1337574 qhpX 4609679 qhpX Azoarcus sp. BH72 putative qinohemoprotein amine dehydrogenase YP_932744.1 1336144 D 62928 CDS YP_932745.1 119897532 4609682 1337576..1337902 1 NC_008702.1 Probable quinohemoprotein amine dehydrogenase,gamma subunit. Homology to qhnDH of P. putida of 54% (sprot|QADG_PSEPK(SRS). Quinoproteins are a class of amine-oxidising enzymes that catalyse the oxidation of biological amines, using quinone as a redox cofactor to store reducing equivalents. Quinohemoprotein amine dehydrogenase (QH-AmDH) from bacteria represents a new class of quinoproteins that contains both quinone and one or two hemes as redox active groups. The presence of extra redox active groups allows for intramolecular electron transfer. QH-AmDH is a heterotrimeric enzyme containing alpha, beta and gamma chains encoded by separate genes. The alpha chain makes contact with both the beta and gamma chains. The small gamma chain forms three intra-chain cross-links via thioester bonds between cysteine and aspartic or glutamic acid residues, thereby encaging the cysteine tryptophylquinone cofactor. The largest chain,alpha, contains two heme c groups. The alpha chain is folded into four domains, domain 1 forming a diheme cytochrome. Domains 2, 3 and 4 are antiparallel beta-barrel structures. no domains predicted. no signal peptide. no TMHs; High confidence in function and specificity; quinohemoprotein amine dehydrogenase subunit gamma 1337902 qhpC 4609682 qhpC Azoarcus sp. BH72 quinohemoprotein amine dehydrogenase subunit gamma YP_932745.1 1337576 D 62928 CDS YP_932746.1 119897533 4609681 1337920..1339065 1 NC_008702.1 Probable quinohemoprotein amine dehydrogenase,alpha subunit. Homology to qhpB of P.putida of 50% (gi|34809688|pdb|1PBY|B(PDB (RCSB))). Quinoproteins are a class of amine-oxidising enzymes that catalyse the oxidation of biological amines, using quinone as a redox cofactor to store reducing equivalents. Quinohemoprotein amine dehydrogenase (QH-AmDH) from bacteria represents a new class of quinoproteins that contains both quinone and one or two hemes as redox active groups. The presence of extra redox active groups allows for intramolecular electron transfer. QH-AmDH is a heterotrimeric enzyme containing alpha, beta and gamma chains encoded by separate genes. The alpha chain makes contact with both the beta and gamma chains. The small gamma chain forms three intra-chain cross-links via thioester bonds between cysteine and aspartic or glutamic acid residues, thereby encaging the cysteine tryptophylquinone cofactor. The largest chain, alpha, contains two heme c groups. The alpha chain is folded into four domains, domain 1 forming a diheme cytochrome. Domains 2, 3 and 4 are antiparallel beta-barrel structures. Interpro (IPR0011044) Quinoprotein amine dehydrogenase, beta chain-like. Signal peptide present. No TMHs.,; High confidence in function and specificity; quinohemoprotein amine dehydrogease subunit beta 1339065 qhpB 4609681 qhpB Azoarcus sp. BH72 quinohemoprotein amine dehydrogease subunit beta YP_932746.1 1337920 D 62928 CDS YP_932747.1 119897534 4609680 complement(1339138..1340523) 1 NC_008702.1 Conserved hypothetical N-acetylmuramoyl-L-alanine amidase. Homology to amiC of R. solanaceraum of 50% (trembl|Q8XWD5) CELL-WALL HYDROLASE PROBABLY INVOLVED IN CELL-WALL HYDROLYSIS SEPTATION OR RECYCLING (BY SIMILARITY). Pfam: N-acetylmuramoyl-L-alanine amidase no signal peptide no TMHs; Family membership; N-acetylmuramoyl-L-alanine amidase 1340523 4609680 azo1243 Azoarcus sp. BH72 N-acetylmuramoyl-L-alanine amidase YP_932747.1 1339138 R 62928 CDS YP_932748.1 119897535 4606758 complement(1340466..1340987) 1 NC_008702.1 Conserved hypothetical protein having uncharacterised P-loop hydrolase, 47% Identity to TrEMBL;Q82WL4,Q63WN1. Has PF02367, Uncharacterised P-loop hydrolase UPF0079;IPR003442; This signature is found in a family of bacterial proteins, which contain a P-loop.; hypothetical protein 1340987 4606758 azo1244 Azoarcus sp. BH72 hypothetical protein YP_932748.1 1340466 R 62928 CDS YP_932749.1 119897536 4606759 1341000..1342058 1 NC_008702.1 Conserved hypothetical electron transport protein. Homology to pa4950 of P. aeruginosa of 63% (trembl|Q9HUL4). Pfam: 4Fe-4S- binding domain Tigrfam: TIG00276: iron-sulfur cluster binding protein, putative no TMHs no signal peptide TIGR00276: iron-sulfur cluster binding pr; Conserved hypothetical protein; electron transport protein 1342058 4606759 azo1245 Azoarcus sp. BH72 electron transport protein YP_932749.1 1341000 D 62928 CDS YP_932750.1 119897537 4606760 complement(1342145..1343311) 1 NC_008702.1 Conserved hypothetical mandelate racemase. Homology to bll6730 of B. japonicum of 76% (trembl|Q89FH0) Mandelate racemase and muconate lactonizing enzyme are two bacterial enzymes involved in aromatic acid catabolism. They catalyze mechanistically distinct reactions yet they are related at the level of their primary, quaternary (homooctamer) and tertiary structures. InterPro: Mandelate racemase/muconate lactonizing enzyme family (IPR001354) Pfam: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain; Mandelate racemase/muconate lactonizing enzyme, C-terminal domain; Specificity unclear; mandelate racemase 1343311 4606760 azo1246 Azoarcus sp. BH72 mandelate racemase YP_932750.1 1342145 R 62928 CDS YP_932751.1 119897538 4606761 1343604..1344530 1 NC_008702.1 Transcriptional regulator, LysR family This protein activates the transcription of the lysA gene encoding diaminopimelate decarboxylase. LysR is also a negative regulator of its own expression. 29% 1 helixturnhelix PF03466 LysR_substrate; 1. HTH reporting nucleic acid binding motif; Specificity unclear; LysR family transcriptional regulator 1344530 4606761 azo1247 Azoarcus sp. BH72 LysR family transcriptional regulator YP_932751.1 1343604 D 62928 CDS YP_932752.1 119897539 4606762 1344671..1345414 1 NC_008702.1 NifY-like protein. Homology to Daro03003760 of Dechloromonas aromatica of 65% (gi|46140358|ref|ZP_00203574.1|(NBCI ENTREZ)). Pfam: Dinitrogenase iron-molybdenum cofactor. This family contains several NIF (B, Y and X) proteins which are involved in the synthesis of an iron-molybdenum cofactors (FeMo-co) in the dinitrogenase enzyme which catalyses the reduction of dinitrogen to ammonium. No signal peptide predicted. No TMHs.; Family membership; NifY protein 1345414 nifY2 4606762 nifY2 Azoarcus sp. BH72 NifY protein YP_932752.1 1344671 D 62928 CDS YP_932753.1 119897540 4609377 1345528..1345794 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology to the data bank. No domains predicted. No signal peptide No TMHs; hypothetical protein 1345794 4609377 azo1249 Azoarcus sp. BH72 hypothetical protein YP_932753.1 1345528 D 62928 CDS YP_932754.1 119897541 4606763 1345875..1346084 1 NC_008702.1 Conserved Hypotheitcal protein. Very strong consideration to be a homolog for SlyX protein. TrEMBL: Q7WMX4, 32% identity. Has PF04102|SlyX;(IPR007236):The SlyX protein has no known function. It is short less than 80 amino acids and is found close to the slyD gene. The SlyX protein has a conserved PPH(Y/W) motif at its C-terminus. The protein may be a coiled-coil structure. No Signal peptide present. No TMH present.; Family membership; hypothetical protein 1346084 4606763 azo1250 Azoarcus sp. BH72 hypothetical protein YP_932754.1 1345875 D 62928 CDS YP_932755.1 119897542 4606764 complement(1346107..1348047) 1 NC_008702.1 Conserved hypothetical peptidase. Homology to cv0266 of C. violaceum of 58% (cvi:CV0266). InterPro: Peptidase family U32 Pfam: Peptidase family U32 no signal peptide no TMHs; Family membership; peptidase 1348047 4606764 azo1251 Azoarcus sp. BH72 peptidase YP_932755.1 1346107 R 62928 CDS YP_932756.1 119897543 4606765 complement(1348202..1348510) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to XCC3430 of Xanthomonas campestris of 38% (trembl:Q8P5B1). Has PF04892, VanZ like family;IPR006976 ; This family contains several examples of the VanZ protein,but also contains examples of phosphotransbutyrylases. VanZ confers low-level resistance to the glycopeptide antibiotic teicoplanin (Te). Analysis of cytoplasmic peptidoglycan precursors, accumulated in the presence of ramoplanin, showed that VanZ-mediated Te resistance does not involve incorporation of a substituent of D-alanine into the peptidoglycan precursors. no signal peptide. 2 TMHs.; Conserved hypothetical protein; hypothetical protein 1348510 4606765 azo1252 Azoarcus sp. BH72 hypothetical protein YP_932756.1 1348202 R 62928 CDS YP_932757.1 119897544 4606766 1348736..1349905 1 NC_008702.1 Similar to the GdhB protein, a putative glucose dehydrogenase-B, periplasmic protein [EC:1.1.5.2], from Synechocystis. SPTR: P73001. Signal peptide:present; hypothetical protein 1349905 4606766 azo1253 Azoarcus sp. BH72 hypothetical protein YP_932757.1 1348736 D 62928 CDS YP_932758.1 119897545 4606767 complement(1349902..1351638) 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to Bcep02001865 of Burkholderia fungorum of 44% (gi|48787085|ref|ZP_00283167.1|(NBCI ENTREZ)). InterPro: IPR003660 HAMP. IPR000160 GGDEF. Pfam: PF00672 HAMP. PF00990 GGDEF domain. TIGRFAM:TIGR00254 putative diguanylate cyclase (GGDEF) domain. Signal P reporting signal peptide. TMHMM reporting 1 transmembrane helices (in signal peptide).; Conserved hypothetical protein; putative signaling protein 1351638 4606767 azo1254 Azoarcus sp. BH72 putative signaling protein YP_932758.1 1349902 R 62928 CDS YP_932759.1 119897546 4606768 complement(1351635..1352795) 1 NC_008702.1 Bacterial high affinity transport systems are involved in active transport of solutes across the cytoplasmic membrane. Similar to trembl|Q986V8 (37%). Pfam (PF01547): Bacterial extracellular solute-binding protein; Family membership; extracellular solute-binding protein 1352795 potD 4606768 potD Azoarcus sp. BH72 extracellular solute-binding protein YP_932759.1 1351635 R 62928 CDS YP_932760.1 119897547 4609598 1352950..1355322 1 NC_008702.1 Putative serin/threonine protein kinase, only very low similarity to SWISSPROT: sprot|PKSC_STRCO (13% Streptomyces coelicolor, serine/threonine protein kinase PksC (EC 2.7.11.1)) / TREMBL: trembl|Q9S478 (13% Myxococcus xanthus, Pkn4). Pfam: PF00069 Pkinase. SMART:SM00221 STYKc (Protein kinase; unclassified specificity). SM00065 GAF (Domain present in phytochromes and cGMP-specific phosphodiesterases).; Family membership; putative serine/threonine protein kinase 1355322 4609598 azo1256 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_932760.1 1352950 D 62928 CDS YP_932761.1 119897548 4606769 1355479..1356123 1 NC_008702.1 Conserved hypothetical protein. Homology to CV1784 of C.violaceum of 40% (tremble:Q7NX45). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1356123 4606769 azo1257 Azoarcus sp. BH72 hypothetical protein YP_932761.1 1355479 D 62928 CDS YP_932762.1 119897549 4606770 1356239..1358347 1 NC_008702.1 hypothetical membrane protein no homology to entire proteins seven transmembrane domains predicted, no signal peptide predicted; Family membership; hypothetical protein 1358347 4606770 azo1258 Azoarcus sp. BH72 hypothetical protein YP_932762.1 1356239 D 62928 CDS YP_932763.1 119897550 4606771 1358370..1359830 1 NC_008702.1 Probable aminoacyl-histidine dipeptidase (EC 3.4.13.3) (Xaa-His dipeptidase) (X-His dipeptidase) (Beta-alanyl-histidine dipeptidase) (Carnosinase) (Peptidase D). Homology to pepD of E. coli of 44%. THIS DIPEPTIDASE HAS SPECIFICITY FOR THE UNUSUAL DIPEPTIDE BETA-ALANYL-L-HISTIDINE. InterPro: Peptidase family M20/M25/M40 (IPR002933) Pfam: Peptidase family M20/M25/M40 no signal peptide no TMHs; High confidence in function and specificity; aminoacyl-histidine dipeptidase 1359830 pepD 4606771 pepD Azoarcus sp. BH72 aminoacyl-histidine dipeptidase YP_932763.1 1358370 D 62928 CDS YP_932764.1 119897551 4609520 complement(1359827..1360354) 1 NC_008702.1 Conserved hypothetical protein. Homology to RPA1575 of R.palustris of 41% (tremble:Q6N9H4). Has PF04536:(IPR007621)Domain of unknown function (DUF477);This is a family of uncharacterised proteins. They are found in both eukarya and eubacteria. No TMHs. No signal peptide.; hypothetical protein 1360354 4609520 azo1260 Azoarcus sp. BH72 hypothetical protein YP_932764.1 1359827 R 62928 CDS YP_932765.1 119897552 4606772 complement(1360403..1361335) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Bll7266 of B. japonicum of 45% (trembel:Q89E21) InterPro:IPR007621; DUF477. Pfam:PF04536; DUF477 Signal peptide present (Signal P predicted). Presence of 2 transmembrane helices.; Conserved hypothetical protein; hypothetical protein 1361335 4606772 azo1261 Azoarcus sp. BH72 hypothetical protein YP_932765.1 1360403 R 62928 CDS YP_932766.1 119897553 4606773 complement(1361353..1361979) 1 NC_008702.1 Putative lipoprotein [lemA],57 % identity (75% similarity) to TrEMBL;Q72TG3,TrEMBL;Q6N9H6(60% identity). No signl peptide or TMH present. Has PF04011:LemA family(IPR007156,lemA);The members of this family are related to the LemA protein. LemA contains an amino terminal predicted transmembrane helix. It has been predicted that the small amino terminus is extracellular [1]. The exact molecular function of this protein is uncertain.; Conserved hypothetical protein; LemA family protein 1361979 lemA1 4606773 lemA1 Azoarcus sp. BH72 LemA family protein YP_932766.1 1361353 R 62928 CDS YP_932767.1 119897554 4609125 1362162..1362374 1 NC_008702.1 Conserved yhpothetical protein. Homology to BP1565 of B.pertussis of 66% (tremble:Q7VY02) Has PF07311:Protein of unknown function (DUF1458)(IPR009923 );This family consists of several hypothetical bacterial proteins as well as one archaeal sequence Q9HPW4. Members of this family are typically of around 70 residues in length. The function of this family is unknown. No signal peptide or TMH present.; hypothetical protein 1362374 4609125 azo1263 Azoarcus sp. BH72 hypothetical protein YP_932767.1 1362162 D 62928 CDS YP_932768.1 119897555 4606774 1362410..1363681 1 NC_008702.1 Putative transmembrane sensor protein, no similarity to other bacterial proteins. Best hits to mus musculus and homo sapiens. Domains (LCCL_dom and TIR) are found in proteins (animal and plants) involved in protection against bacterial infection. InterPro: IPR004043 LCCL_dom. IPR000157 TIR. TMHMM reporting 1 transmembrane helices.; putative transmembrane sensor protein 1363681 4606774 azo1264 Azoarcus sp. BH72 putative transmembrane sensor protein YP_932768.1 1362410 D 62928 CDS YP_932769.1 119897556 4606775 complement(1363688..1363981) 1 NC_008702.1 Hypothetical protein, 26% identity(49% similarity) to TrEMBL;Q8XT66. TrEMBL;Q7P1L8(34% identity). No domains,repeats, motifs or features present.; hypothetical protein 1363981 4606775 azo1265 Azoarcus sp. BH72 hypothetical protein YP_932769.1 1363688 R 62928 CDS YP_932770.1 119897557 4606776 complement(1364049..1365062) 1 NC_008702.1 Putative chaperone protein dnaJ. homology to dnaJ of E. coli of 32% (sprot|DNAJ_ECOLI) Acts as a co-chaperone. Stimulates jointly with grpE the ATPase activity of dnaK (By similarity). Pfam: DnaJ domain, DnaJ central domain (4 repeats), DnaJ Cterminal region no singal peptide no TMHs; Family membership; putative chaperone protein DnaJ 1365062 dnaJ2 4606776 dnaJ2 Azoarcus sp. BH72 putative chaperone protein DnaJ YP_932770.1 1364049 R 62928 CDS YP_932771.1 119897558 4608635 1365220..1365705 1 NC_008702.1 Entry name TREMBL:Q7W380 Prim. accession # Q7W380 Identities = 111/150 (74%) InterPro IPR006683; Thioestr_supf. Pfam PF03061; 4HBT; 1. Prediction: Non-secretory protein Signal peptide probability: 0.179 Number of predicted TMHs: 0; Family membership; acyl-CoA thioester hydrolase 1365705 4608635 azo1267 Azoarcus sp. BH72 acyl-CoA thioester hydrolase YP_932771.1 1365220 D 62928 CDS YP_932772.1 119897559 4606777 1365702..1365947 1 NC_008702.1 Conserved hypothetical protein. Homology to PBPRB0126 of Photobacterium profundum of 54% (trembl:Q6LL06). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1365947 4606777 azo1268 Azoarcus sp. BH72 hypothetical protein YP_932772.1 1365702 D 62928 CDS YP_932773.1 119897560 4606778 complement(1365959..1367140) 1 NC_008702.1 non-folate utilizing enzyme, catalyzes the production of beta-formyl glycinamide ribonucleotide from formate, ATP, and beta-GAR and a side reaction producing acetyl phosphate and ADP from acetate and ATP; involved in de novo purine biosynthesis; phosphoribosylglycinamide formyltransferase 2 1367140 purT 4606778 purT Azoarcus sp. BH72 phosphoribosylglycinamide formyltransferase 2 YP_932773.1 1365959 R 62928 CDS YP_932774.1 119897561 4609664 1367335..1368981 1 NC_008702.1 Probable Branched-chain amino acid aminotransferase (EC 2.6.1.42) (Transaminase B) (BCAT). thrC: threonine synthase.; Function unclear; hypothetical protein 1368981 4609664 azo1270 Azoarcus sp. BH72 hypothetical protein YP_932774.1 1367335 D 62928 CDS YP_932775.1 119897562 4606779 complement(1368995..1369639) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pa3214 of P. aeruginosa of 52% (trembl|Q9HZ26). Pfam: DUF330 signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 1369639 4606779 azo1271 Azoarcus sp. BH72 hypothetical protein YP_932775.1 1368995 R 62928 CDS YP_932776.1 119897563 4606780 complement(1369636..1370577) 1 NC_008702.1 Conserved hypothetical secreted protein. Similar to TREMBL:Q9HZ27 (53% identity); TREMBL:Q88RI9 (46% identity); TREMBL:Q8PGP4 (33% identity). Pfam (PF02470): mce related protein. SignalP reporting signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 1370577 4606780 azo1272 Azoarcus sp. BH72 hypothetical protein YP_932776.1 1369636 R 62928 CDS YP_932777.1 119897564 4606781 complement(1370579..1371361) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q8PGP5 (60% identity); TREMBL:Q9PDS7 (57% identity). InterPro (IPR003593): AAA ATPase. InterPro (IPR003439): ABC transporter. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Specificity unclear; putative ABC transporter ATP-binding protein 1371361 4606781 azo1273 Azoarcus sp. BH72 putative ABC transporter ATP-binding protein YP_932777.1 1370579 R 62928 CDS YP_932778.1 119897565 4606782 complement(1371358..1372500) 1 NC_008702.1 TREMBL:Q88RI7 (56% identity); TREMBL:Q8PGP6 (42% identity). Pfam (PF02405): Domain of unknown function DUF140. TIGRFAM (TIGR00056): Conserved hypothetical protein. TMHMM reporting six transmembrane helices.; Specificity unclear; putative ABC transporter permease 1372500 4606782 azo1274 Azoarcus sp. BH72 putative ABC transporter permease YP_932778.1 1371358 R 62928 CDS YP_932779.1 119897566 4606783 complement(1372520..1374184) 1 NC_008702.1 ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence; putative ABC transporter ATP-binding protein 1374184 yjjK 4606783 yjjK Azoarcus sp. BH72 putative ABC transporter ATP-binding protein YP_932779.1 1372520 R 62928 CDS YP_932780.1 119897567 4610190 1374585..1375484 1 NC_008702.1 Putative methyltransferase Rv0089/MT0098/Mb0092 (EC 2.1.1.-). Methyl transfer from the ubiquitous S-adenosyl-L-methionine (AdoMet) to either nitrogen, oxygen or carbon atoms is frequently employed in diverse organisms TREMBL:AAS95087-36%identity,50% similarity InterPro: SAM (and some other nucleotide) binding motif rrmJ: ribosomal RNA large subunit methylation TIGRFAM:Transcription regulator, AsR family, Ubi/COQ family.; Family membership; putative O-methyltransferase 1375484 4610190 azo1276 Azoarcus sp. BH72 putative O-methyltransferase YP_932780.1 1374585 D 62928 CDS YP_932781.1 119897568 4606784 complement(1375505..1375996) 1 NC_008702.1 conserved hypothetical glutathione peroxidase. Homolgy to pp1874 pf P. putida of 66% (trembl|Q88LQ5) Glutathione peroxidase (GSHPx), an enzyme whose principal function is to protect against damage from endogenously-formed hydroxyperoxides, catalyses the reduction of hydroxyperoxides by glutathione Pfam: Glutatione peroxidase no signal peptide no TMHs; Family membership; glutathione peroxidase 1375996 4606784 azo1277 Azoarcus sp. BH72 glutathione peroxidase YP_932781.1 1375505 R 62928 CDS YP_932782.1 119897569 4606785 complement(1376095..1378737) 1 NC_008702.1 EAL/GGDEF/PAS/PAC-domain containing signalling protein,; Conserved hypothetical protein; EAL/GGDEF/PAS/PAC-domain-containing signalling protein 1378737 4606785 azo1278 Azoarcus sp. BH72 EAL/GGDEF/PAS/PAC-domain-containing signalling protein YP_932782.1 1376095 R 62928 CDS YP_932783.1 119897570 4606786 complement(1378734..1379822) 1 NC_008702.1 Probable cytochrome c peroxidase (EC 1.11.1.5), an electron-transfer proteins having one or several haem c groups, bound to the protein by one or, more generally,two thioether bonds involving sulphydryl groups of cysteine residues. CytC possess a wide range of properties and function in a large number of different redox processes. Similar to yhjA from E.coli.; High confidence in function and specificity; putative cytochrome C peroxidase 1379822 4606786 azo1279 Azoarcus sp. BH72 putative cytochrome C peroxidase YP_932783.1 1378734 R 62928 CDS YP_932784.1 119897571 4606787 complement(1379865..1380641) 1 NC_008702.1 Ferredoxin-NADP+ reductase (EC 1.18.1.2) Homology to fpr of A. vinelandii of 79% (sprot|FENR_AZOVI). CATALYTIC ACTIVITY: Reduced ferredoxin + NADP(+) = oxidized ferredoxin + NADPH. Pfam: Oxidoreductase FAD-binding doamin; Oxidoreductase NAD-binding domain no signal peptide no TMHs; High confidence in function and specificity; ferredoxin-NADP+ reductase 1380641 fpr1 4606787 fpr1 Azoarcus sp. BH72 ferredoxin-NADP+ reductase YP_932784.1 1379865 R 62928 CDS YP_932785.1 119897572 4608824 1380741..1381667 1 NC_008702.1 Probable transcriptional regulator, LysR family proteins.57% Identity to TrEMBL;Q9HYK6, Q87XZ5, Q88MD6. Has PF03466, LysR substrate binding domain;IPR005119,LysR_subst; The structure of this domain is known and is IPR000847, HTH_LysR; Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif. These proteins are very diverse, but for convenience may be grouped into subfamilies on the basis of sequence similarity. One such family, the lysR family,groups together a range of proteins, including ampR, catM,catR, cynR, cysB, gltC, iciA, ilvY, irgB, lysR, metR,mkaC, mleR, nahR, nhaR, nodD, nolR, oxyR, pssR, rbcR,syrM, tcbR, tfdS and trpI. The majority of these proteins appear to be transcription activators and most are known to negatively regulate their own expression. All possess a potential HTH DNA-binding motif towards their N-termini.; High confidence in function and specificity; LysR family transcriptional regulator 1381667 4608824 azo1281 Azoarcus sp. BH72 LysR family transcriptional regulator YP_932785.1 1380741 D 62928 CDS YP_932786.1 119897573 4606788 complement(1381825..1384443) 1 NC_008702.1 Beta-(1-3)-glucosyl transferase, involved in the synthesis of the cyclic beta-(1,3),(1,6)-D-glucan. 36% Glyco_trans_2. Pfam:PF00535; Glycos_transf_2. Signal peptide: present. TMhelix:9.; Function unclear; putative Beta-(1-3)-glucosyl transferase 1384443 ndvB 4606788 ndvB Azoarcus sp. BH72 putative Beta-(1-3)-glucosyl transferase YP_932786.1 1381825 R 62928 CDS YP_932787.1 119897574 4609351 1384729..1385121 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PP1739 of P. putida of 30% (trembl|Q8EA80) No domains predicted. signal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 1385121 4609351 azo1283 Azoarcus sp. BH72 hypothetical protein YP_932787.1 1384729 D 62928 CDS YP_932788.1 119897575 4606789 complement(1385065..1386660) 1 NC_008702.1 Glucan 1-3-beta-glucosidase precursor.(Exo-13-beta-glucanase).Glucanases possibly play a role in cell expansion during growth in cell-cell fusion during mating and in spore release during sporulation. This enzyme may be involved in beta- glucan degradation and also function biosynthetically as a transglycosylase. 47%; Function unclear; putative glucan 1,3-beta-glucosidase 1386660 ndvC 4606789 ndvC Azoarcus sp. BH72 putative glucan 1,3-beta-glucosidase YP_932788.1 1385065 R 62928 CDS YP_932789.1 119897576 4609352 complement(1386740..1389619) 1 NC_008702.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; glycine dehydrogenase 1389619 gcvP 4609352 gcvP Azoarcus sp. BH72 glycine dehydrogenase YP_932789.1 1386740 R 62928 CDS YP_932790.1 119897577 4608871 complement(1389681..1390064) 1 NC_008702.1 part of multienzyme complex composed of H, L, P, and T proteins which catalyzes oxidation of glycine to yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a reduced pyridine nucleotide; protein H is involved in transfer of methylamine group from the P to T protein; covalently bound to a lipoyl cofactor; glycine cleavage system protein H 1390064 gcvH 4608871 gcvH Azoarcus sp. BH72 glycine cleavage system protein H YP_932790.1 1389681 R 62928 CDS YP_932791.2 228906473 4608870 complement(1390115..1391206) 1 NC_008702.1 catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein; glycine cleavage system aminomethyltransferase T 1391206 gcvT 4608870 gcvT Azoarcus sp. BH72 glycine cleavage system aminomethyltransferase T YP_932791.2 1390115 R 62928 CDS YP_932792.1 119897579 4608235 1397768..1398982 1 NC_008702.1 Probable nitrate transporters are involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. NarK is polytopic membrane protein with 12 transmembrane domains which is involved in nitrate uptake and nitrite excretion and is thought to function as a nitrate/nitrite antiporter. At low concentrations of nitrate, NarK mediates the electrogenic excretion of nitrite rather than nitrate/nitrite exchange., 90% similarity to a probable nitrate transporter protein in Azoarcus sp. EbN1., InterPRo:04737: nitrate transporterSignal peptide no present, TMHx:12 ,; High confidence in function and specificity; putative nitrate transporter 1398982 narK 4608235 narK Azoarcus sp. BH72 putative nitrate transporter YP_932792.1 1397768 D 62928 CDS YP_932793.1 119897580 4609340 1399088..1400770 1 NC_008702.1 Putative serine/threonine protein kinase,; Family membership; putative serine/threonine protein kinase 1400770 4609340 azo1289 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_932793.1 1399088 D 62928 CDS YP_932794.1 119897581 4606790 complement(1400786..1401718) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA98 of Azoarcus sp. EbN1 of 67% (gnl|keqq|eba:ebA983(KEGG)). No domains predicted. No signal peptide. No TMHs; hypothetical protein 1401718 4606790 azo1290 Azoarcus sp. BH72 hypothetical protein YP_932794.1 1400786 R 62928 CDS YP_932795.1 119897582 4606791 complement(1401860..1402312) 1 NC_008702.1 Putative phosphohistidine phosphatase, SixA. TIGRFAM: TIGR00249; sixA. Phosphohistidine phosphatase sixA (EC 3.1.3.-) (RX6). EXHIBITS PHOSPHOHISTIDINE PHOSPHATASE ACTIVITY TOWARDS THE HPT DOMAIN OF THE ARCB SENSOR INVOLVED IN THE MULTISTEP HIS- ASP PHOSPHORELAY.; Specificity unclear; putative phosphohistidine phosphatase 1402312 sixA 4606791 sixA Azoarcus sp. BH72 putative phosphohistidine phosphatase YP_932795.1 1401860 R 62928 CDS YP_932796.1 119897583 4609871 complement(1402375..1403868) 1 NC_008702.1 Conserved hypothetical protein,40% identity to TrEMBL;Q88AR4 Has PF01928|CYTH domain(IPR008172);These sequences are functionally identified as members of the adenylate cyclase family, which catalyses the conversion of ATP to 3',5'-cyclic AMP and pyrophosphate. Has PF05235:CHAD domain(IPR007899);The CHAD domain is an alpha-helical domain functionally associated with the CYTH domains. It has conserved histidines that may chelate metals.; adenylate cyclase 1403868 4609871 azo1292 Azoarcus sp. BH72 adenylate cyclase YP_932796.1 1402375 R 62928 CDS YP_932797.1 119897584 4606792 complement(1403946..1406627) 1 NC_008702.1 catalyzes the ATP-dependent addition of AMP to a subunit of glutamine synthetase; also catalyzes the reverse reaction - deadenylation; adenylation/deadenylation of glutamine synthetase subunits is important for the regulation of this enzyme; bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase 1406627 glnE 4606792 glnE Azoarcus sp. BH72 bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase YP_932797.1 1403946 R 62928 CDS YP_932798.1 119897585 4608897 1406776..1410699 1 NC_008702.1 Conserved hypothetical protein. Homology to cv2096 of C. violaceum of 32% (trembl|Q7NW94(SRS) no domains predicted no signal peptide 1 TMHs; hypothetical protein 1410699 4608897 azo1294 Azoarcus sp. BH72 hypothetical protein YP_932798.1 1406776 D 62928 CDS YP_932799.1 119897586 4606793 1410696..1411553 1 NC_008702.1 conserved hypothetical carbon-nitrogen hydrolase. Homolog to bb1933 of B. bronchiseptica (trembl|Q7WL17). InterPro: Carbon-nitrogen hydrolase (IPR003010) Pfam: Carbon-nitorgen hydrolase no signal peptide no TMHs; Conserved hypothetical protein; carbon-nitrogen hydrolase 1411553 4606793 azo1295 Azoarcus sp. BH72 carbon-nitrogen hydrolase YP_932799.1 1410696 D 62928 CDS YP_932800.1 119897587 4606794 1411624..1413069 1 NC_008702.1 Probable TldD protein. Homology to tldD of E. coli of 59% (sprot|TLDD_ECOLI). Suppresses the inhibitory activity of the carbon storage regulator (csrA). Pfam: putative modulator of DNA gyrase no signal peptide no TMHs; Function unclear; TldD protein 1413069 tldD 4606794 tldD Azoarcus sp. BH72 TldD protein YP_932800.1 1411624 D 62928 CDS YP_932801.1 119897588 4609951 complement(1413077..1413520) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3985 of C.violaceum of 42% (tremble:Q7NR00). Has PF07025:(IPR010745)Protein of unknown function (DUF1316);This family consists of several hypothetical bacterial proteins of around 150 residues in length. The function of this family is unknown. No signal peptide or TMH present.; hypothetical protein 1413520 4609951 azo1297 Azoarcus sp. BH72 hypothetical protein YP_932801.1 1413077 R 62928 CDS YP_932802.1 119897589 4606795 complement(1413533..1414231) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv3984 of C. violaceum of 47% (trembl|Q7NR01(SRS)) no domains predicted no signal peptide 1 TMH; Conserved hypothetical protein; hypothetical protein 1414231 4606795 azo1298 Azoarcus sp. BH72 hypothetical protein YP_932802.1 1413533 R 62928 CDS YP_932803.1 119897590 4606796 complement(1414245..1415591) 1 NC_008702.1 Hypothetical protein,50% identity (67% similarity)to TrEMBL;Q7NR02. TrEMBL;Q88IA6(39%),Q8XRU1(37%). Has PF05936(IPR010263):Bacterial protein of unknown function (DUF876);This family consists of a series of hypothetical bacterial sequences of unknown function. No Signal Peptide or TMH reported present.; Function unclear; hypothetical protein 1415591 4606796 azo1299 Azoarcus sp. BH72 hypothetical protein YP_932803.1 1414245 R 62928 CDS YP_932804.1 119897591 4606797 complement(1415603..1416217) 1 NC_008702.1 Hypothetical protein,34% identity (52% similarity) to TrEMBL;Q7NR03. No domains, repeats, motifs or features were predicted with confidence.; Function unclear; hypothetical protein 1416217 4606797 azo1300 Azoarcus sp. BH72 hypothetical protein YP_932804.1 1415603 R 62928 CDS YP_932805.1 119897592 4606798 1416388..1417059 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein with the data bank. Pfam: D-alanyl-D-alanine carboxypeptidase (101-209 aa) no signal peptide 1 TMHs; hypothetical protein 1417059 4606798 azo1301 Azoarcus sp. BH72 hypothetical protein YP_932805.1 1416388 D 62928 CDS YP_932806.1 119897593 4606799 1417056..1420748 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV3712 of Chromobacterium violaceum of 35% (trembl|Q7NR05) Signal P reporting Signal Peptide Present. TMHMM2 reporting 2 TMH's present. Has PF06761:(IPR009612)ImcF-related;This family represents a conserved region within several bacterial proteins that resemble ImcF, which has been proposed [1] to be involved in Vibrio cholerae cell surface reorganisation, resulting in increased adherence to epithelial cells and increased conjugation frequency. Note that many family members are hypothetical proteins. Has PF06744,(IPR010623)Protein of unknown function (DUF1215);This family represents a conserved region situated towards the C-terminal end of several hypothetical bacterial proteins of unknown function. A few members resemble the ImcF protein, which has been proposed to be involved in Vibrio cholerae cell surface reorganisation that results in increased adherence to epithelial cells line and increased conjugation frequency.; Conserved hypothetical protein; hypothetical protein 1420748 4606799 azo1302 Azoarcus sp. BH72 hypothetical protein YP_932806.1 1417056 D 62928 CDS YP_932807.1 119897594 4606800 1420836..1421360 1 NC_008702.1 Hypothetical protein,50% identity (66% Similarity) to TrEMBL;Q8XRT8. TrEMBL;Q7NR06(60% identity,74% similarity). Has PF05591;Protein of unknown function (DUF770)(IPR008312)This family consists of several proteins of unknown function from various bacterial species. No Signal peptide or TMH present.; Function unclear; hypothetical protein 1421360 4606800 azo1303 Azoarcus sp. BH72 hypothetical protein YP_932807.1 1420836 D 62928 CDS YP_932808.1 119897595 4606801 1421344..1422837 1 NC_008702.1 Hypothetical protein,63% identity (81% similarity) to TrEMBL;Q8XRT7. TrEMBL;Q7NR07(73% identity,86% similarity). Has (IPR010269 )PF05943:Protein of unknown function (DUF877);This family consists of a number of uncharacterised bacterial proteins. The function of this family is unknown. No Signal peptide or TMH present.; Function unclear; hypothetical protein 1422837 4606801 azo1304 Azoarcus sp. BH72 hypothetical protein YP_932808.1 1421344 D 62928 CDS YP_932809.1 119897596 4606802 1422881..1423378 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3977 of C.violaceum of 77% (tremble:Q7NR08). Has PF05638:(IPR008514)Protein of unknown function (DUF796);This family consists of several bacterial proteins of unknown function. No signal peptide or TMH present.; hypothetical protein 1423378 4606802 azo1305 Azoarcus sp. BH72 hypothetical protein YP_932809.1 1422881 D 62928 CDS YP_932810.1 119897597 4606803 1423378..1423854 1 NC_008702.1 Hypothetical secreted protein. Homology to CV3976 of Chromobacterium violaceum of 26% (trembl|Q7NR09(SRS)). No TMHs Signal Peptide Present. Has PF00691:OmpA family;(IPR006665 OmpA/MotB):The Pfam entry also includes MotB and related proteins which are not included in the Prosite family.; hypothetical protein 1423854 4606803 azo1306 Azoarcus sp. BH72 hypothetical protein YP_932810.1 1423378 D 62928 CDS YP_932811.1 119897598 4606804 1423911..1426628 1 NC_008702.1 Probable vgr-related protein,41% identity (55% similarity) to TrEMBL;Q7NY43. TrEMBL;Q8XSF8 Has (IPR006533 Rhs_Vgr)PF04524:Protein of unknown function, DUF586;This family contains a conserved region in several bacterial proteins of unknown function. Coils2 program predicts presence of a coiled coil.; Vgr related protein 1426628 4606804 azo1307 Azoarcus sp. BH72 Vgr related protein YP_932811.1 1423911 D 62928 CDS YP_932812.1 119897599 4606805 1426635..1428473 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3969 of C.violaceum of 56% (tremble: Q7NR16). Has (IPR010272)PF05947:Bacterial protein of unknown function (DUF879);This family consists of several hypothetical bacterial proteins of unknown function. No signal peptide or TMH present.; hypothetical protein 1428473 4606805 azo1308 Azoarcus sp. BH72 hypothetical protein YP_932812.1 1426635 D 62928 CDS YP_932813.1 119897600 4606806 1428437..1429453 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3967 of C.violaceum of 45% (tremble:Q7NR18) Has (IPR010732)PF06996:Protein of unknown function (DUF1305);This family consists of several hypothetical bacterial proteins of around 300 residues in length. The function of this family is unknown although one member (Q93IT4) from Salmonella enterica is thought to be involved in virulence. No signal peptide or TMH reported present.; hypothetical protein 1429453 4606806 azo1309 Azoarcus sp. BH72 hypothetical protein YP_932813.1 1428437 D 62928 CDS YP_932814.1 119897601 4606807 complement(1429450..1430565) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3963 of C.violaceum of 37% (tremble:Q7NR22). Has (IPR01065)PF06812:ImpA-related N-terminal;This family represents a conserved region located towards the N-terminal end of ImpA and related proteins. ImpA is an inner membrane protein, which has been suggested to be involved with proteins that are exported and associated with colony variations in Actinobacillus actinomycetemcomitans. Note that many family members are hypothetical proteins. No signal peptide present. No TMH present.; hypothetical protein 1430565 4606807 azo1310 Azoarcus sp. BH72 hypothetical protein YP_932814.1 1429450 R 62928 CDS YP_932815.1 119897602 4606808 complement(1430572..1430835) 1 NC_008702.1 conserved hypothetical protein Has 2 PF05488 PAAR motif(IPR008727);This motif is found usually in pairs in a family of bacterial membrane proteins. It is also found as a triplet of tandem repeats comprising the entire length in a another family of hypothetical proteins.; Function unclear; hypothetical protein 1430835 4606808 azo1311 Azoarcus sp. BH72 hypothetical protein YP_932815.1 1430572 R 62928 CDS YP_932816.1 119897603 4606809 1431039..1432133 1 NC_008702.1 catalyzes the formation of 5-methyl-uridine at position 54 in all tRNAs; tRNA (uracil-5-)-methyltransferase 1432133 4606809 azo1312 Azoarcus sp. BH72 tRNA (uracil-5-)-methyltransferase YP_932816.1 1431039 D 62928 CDS YP_932817.1 119897604 4606810 1432168..1432506 1 NC_008702.1 PhnA protein: Phosphonoacetate hydrolase is a novel carbon-phosphorus bond cleavage enzyme.The phnA gene is part of a large operon in Escherichia coli associated with alkylphosphonate uptake and carbon-phosphorus bond cleavage. PhnA is found in both gram positive 69% PhnA.PhnA protein Pfam:PF03831; PhnA; 1. TIGRFAMs:TIGR00686; phnA; 1.; High confidence in function and specificity; putative phosphonoacetate hydrolase 1432506 phnA 4606810 phnA Azoarcus sp. BH72 putative phosphonoacetate hydrolase YP_932817.1 1432168 D 62928 CDS YP_932818.1 119897605 4609549 complement(1432522..1432893) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA3072 Azoarcus sp. EbN1 of 61% (gnl|keqq|eba:ebA3072(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1432893 4609549 azo1314 Azoarcus sp. BH72 hypothetical protein YP_932818.1 1432522 R 62928 CDS YP_932819.1 119897606 4606811 complement(1432952..1433749) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to blr8071 of B. japonicum of 56% (trembl|Q89BS7). No domains predicted signal peptide probable 6 TMHs; Conserved hypothetical protein; hypothetical protein 1433749 4606811 azo1315 Azoarcus sp. BH72 hypothetical protein YP_932819.1 1432952 R 62928 CDS YP_932820.1 119897607 4606812 complement(1433746..1434351) 1 NC_008702.1 47% AAA_ATPase.IPR003439; ABC_transporter.IPR008995. Pfam:PF00005; ABC_tran; 1. SMART:SM00382; AAA; 1. non-secretory protein; Function unclear; putative ATP binding protein 1434351 4606812 azo1316 Azoarcus sp. BH72 putative ATP binding protein YP_932820.1 1433746 R 62928 CDS YP_932821.1 119897608 4606813 1434635..1435387 1 NC_008702.1 Conserved hypothetical protein. Homology to CCO1214 of Campylobacter coli of 38% (gi|57168023|ref|ZP_00367162.1|(NBCI ENTREZ)). Has PF07021, Methionine biosynthesis protein MetW;IPR010743; This family consists of several bacterial and one archaeal methionine biosynthesis MetW proteins. Biosynthesis of methionine from homoserine in Pseudomonas putida takes place in three steps. The first step is the acylation of homoserine to yield an acyl-L-homoserine. This reaction is catalysed by the products of the metXW genes and is equivalent to the first step in enterobacteria, gram-positive bacteria and fungi, except that in these microorganisms the reaction is catalysed by a single polypeptide (the product of the metA gene in Escherichia coli and the met5 gene product in Neurospora crassa). In Pseudomonas putida, as in gram-positive bacteria and certain fungi, the second and third steps are a direct sulfhydrylation that converts the O-acyl-L-homoserine into homocysteine and further methylation to yield methionine. The latter reaction can be mediated by either of the two methionine synthetases present in the cells. No signal peptide. No TMHs.; hypothetical protein 1435387 4606813 azo1317 Azoarcus sp. BH72 hypothetical protein YP_932821.1 1434635 D 62928 CDS YP_932822.1 119897609 4606814 complement(1435529..1436443) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS04440 of Ralstonia solanacearum of 40% (trembl|Q8Y277) TMHMM2 reporting 2 TMH's present. Signal peptide Present. Has PF04280;Tim44-like domain(IPR007379):Tim44 is an essential component of the machinery that mediates the translocation of nuclear-encoded proteins across the mitochondrial inner membrane. Tim44 is thought to bind phospholipids of the mitochondrial inner membrane both by electrostatic interactions and by penetrating the polar head group region. This family includes the C-terminal region of Tim44 that has been shown to form a stable proteolytic fragment in yeast. This region is also found in a set of smaller bacterial proteins. The molecular function of the bacterial members of this family is unknown but transport seems likely.; Conserved hypothetical protein; hypothetical protein 1436443 4606814 azo1318 Azoarcus sp. BH72 hypothetical protein YP_932822.1 1435529 R 62928 CDS YP_932823.1 119897610 4606815 complement(1436614..1437468) 1 NC_008702.1 Hypothetical protein Rv1403c/MT1447/Mb1438c precursor. TREMBL:Q92U99_Putative methyl transferase, S-adenosyl-L-methionine (SAM)-29% identity, 42% similarity. InterPro: ubiE/COQ5 methyltransferase pimt: protein-L-isoaspartate O-methyltr Pfam: ubiE/COQ5 methyl transferase family, Dihydrodipicolinate synthetase family, TerD (Bacterial stress protein domains). HTH_1 predicted regulatory helix turn helix. TIGRFAM: moaC, met p-daseII; Specificity unclear; putative methyltransferase 1437468 4606815 azo1319 Azoarcus sp. BH72 putative methyltransferase YP_932823.1 1436614 R 62928 CDS YP_932824.1 119897611 4606816 1437635..1438639 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 1438639 4606816 azo1320 Azoarcus sp. BH72 AraC family transcriptional regulator YP_932824.1 1437635 D 62928 CDS YP_932825.1 119897612 4606817 1438850..1439548 1 NC_008702.1 Conserved hypothetical protein. Homology to Psyc03001842 of Psychrobacter sp. 273-4 of 57% (gi|52853589|ref|ZP_00145853.2|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1439548 4606817 azo1321 Azoarcus sp. BH72 hypothetical protein YP_932825.1 1438850 D 62928 CDS YP_932826.1 119897613 4606818 1439761..1440165 1 NC_008702.1 hypothetical protein 1440165 4606818 azo1322 Azoarcus sp. BH72 hypothetical protein YP_932826.1 1439761 D 62928 CDS YP_932827.1 119897614 4606819 1440382..1441617 1 NC_008702.1 Probable protein kinase InterPro: Protein kinase,Protein kinase-like; protein kinase 1441617 4606819 azo1323 Azoarcus sp. BH72 protein kinase YP_932827.1 1440382 D 62928 CDS YP_932828.1 119897615 4606820 complement(1441791..1442165) 1 NC_008702.1 C-terminal part of GTP-binding protein hflX. TREMBL:Q7NU63: 66% identity; 83% similarity ( the protein of query matches only with C-terminal part (124 aminoacids) of putative GTP binding proteins in the database) InterPro:IPR006073; GTP1_OBG. PRINTS:PR00326; GTP1OBG Pfam:Sigma54_activat: Sigma-54 interaction domain Absence of transmembrane helices (TMHMM predicted); Function unclear; GTP binding protein 1442165 4606820 azo1324 Azoarcus sp. BH72 GTP binding protein YP_932828.1 1441791 R 62928 CDS YP_932829.1 119897616 4606821 complement(1442281..1443378) 1 NC_008702.1 Region start changed from 1443402 to 1443378 (-24 bases), , Changed start codon from att to next atg; hypothetical protein 1443378 4606821 azo1325 Azoarcus sp. BH72 hypothetical protein YP_932829.1 1442281 R 62928 CDS YP_932830.1 119897617 4606822 1443478..1443669 1 NC_008702.1 Hypothetical Protein. No domains, repeats, motifs or features could be predicted above threshold scores.; hypothetical protein 1443669 4606822 azo1326 Azoarcus sp. BH72 hypothetical protein YP_932830.1 1443478 D 62928 CDS YP_932831.1 119897618 4606823 complement(1443843..1445237) 1 NC_008702.1 GTP-binding protein hflX. trembl:Q7NU63:77% identity, 83% similarity The proteins contain GTP-binding motifs and are GTP1_OBG. PRINTS:PR00326; GTP1OBG Pfam:MMR_HSR1:GTPase of unknown function thdF: tRNA modification GTPase TrmE No signal peptide present (SignalP predicted). No transmembrane helices present (TMHMM predicted); Family membership; GTP-binding protein HflX 1445237 hflX2 4606823 hflX2 Azoarcus sp. BH72 GTP-binding protein HflX YP_932831.1 1443843 R 62928 CDS YP_932832.1 119897619 4610017 1445591..1446979 1 NC_008702.1 Conserved hypothetical protein. Fusion Protein of bacterial extracellular solute-binding protein and transglycosylase Homology to pp1036 of P. putida of 37% (trembl|Q88P17) InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR001638); solute binding protein/glutamate receptor (IPR001311); SLT domain (IPR000189) Pfam: Bacterial extracellular solute-binding protein; Transglycosylase SLT domain signal peptide no TMHs recN: DNA repair protein RecN; hypothetical protein 1446979 4610017 azo1328 Azoarcus sp. BH72 hypothetical protein YP_932832.1 1445591 D 62928 CDS YP_932833.1 119897620 4606824 complement(1446976..1448064) 1 NC_008702.1 Putative amino-acid ABC transporter permease. Homology to aapM of R. leguminosarum of 38% (sprot|AAPM_RHILV) PROBABLY PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM YDHWXYZ FOR AN AMINO ACID; PROBABLY RESPONSIBLE FOR THE TRANSLOCATION OF THE SUBSTRATE ACROSS THE MEMBRANE. Pfam: binding-protein-dependent transport system signal peptide probable 8 TMHs; Family membership; putative amino acid permease 1448064 aapM 4606824 aapM Azoarcus sp. BH72 putative amino acid permease YP_932833.1 1446976 R 62928 CDS YP_932834.1 119897621 4608241 complement(1448061..1449080) 1 NC_008702.1 Putative amino acid permease. Homology to aapQ of R. leguminosarum of 35% (sprot|AAPQ_RHILVast). PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR L-AMINO ACIDS. AFFECTS THE UPTAKE AS WELL AS EFFLUX OF THESE AMINO ACIDS. PROBABLY RESPONSIBLE FOR THE TRANSLOCATION OF THE SUBSTRATE ACROSS THE MEMBRANE. InterPro: Binding-protein-dependent transport systems inner membrane component (IPR000515) Pfam: Binding-protein -dependent transport system 1 HTH no signal peptide probable 4 TMHs; Family membership; putative amino acid permease 1449080 aapQ 4608241 aapQ Azoarcus sp. BH72 putative amino acid permease YP_932834.1 1448061 R 62928 CDS YP_932835.1 119897622 4608242 complement(1449038..1450072) 1 NC_008702.1 Putative amino acid ABC transporter periplasmic binding protein. Homology to aapJ of R. leguminosarum of 52% (sprot|AAPJ_RHILV). PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR L-AMINO ACIDS AFFECTS THE UPTAKE AS WELL AS EFFLUX OF THESE AMINO ACIDS. InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR001638); solute binding protein/glutamate receptor (IPR001311) Pfam: Bacterial extracellular solute-binding proteins signal peptide no TMHs TIGR00045: conserved hypothetical prote; High confidence in function and specificity; putative amino acid-binding protein 1450072 aapJ 4608242 aapJ Azoarcus sp. BH72 putative amino acid-binding protein YP_932835.1 1449038 R 62928 CDS YP_932836.1 119897623 4608240 complement(1450197..1451132) 1 NC_008702.1 HTH-type transcriptional regulator cbl (Cys regulon transcriptional activator) Belongs to the LysR family of transcriptional regulators. THIS PROTEIN IS A POSITIVE REGULATOR OF GENE EXPRESSION FOR THE CYSTEINE REGULON. THE INDUCER FOR CYSB IS N-ACETYLSERINE (BY SIMILARITY). ribD_Cterm: riboflavin-specific deami 55% similarity to the HTH-type transcriptional regulator cbl, E. coli SWISSPROT:CBL_ECOLI IPR000847; HTH_LysR. IPR005119; LysR_subst. PF00126; HTH_1; 1. helix-turn-helix PF03466; LysR_substrate; 1. HTH reporting nucleic acid binding motif; High confidence in function and specificity; transcriptional regulator CysB-like protein 1451132 cbl 4608240 cbl Azoarcus sp. BH72 transcriptional regulator CysB-like protein YP_932836.1 1450197 R 62928 CDS YP_932837.1 119897624 4608436 complement(1451135..1452220) 1 NC_008702.1 Sulfate/thiosulfate import ATP-binding protein cysA (EC 3.6.3.25) (Sulfate-transporting ATPase). Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system (By similarity). InterPro: AAA ATPase superfamily ruvB: Holliday junction DNA helicase Ru; High confidence in function and specificity; putative sulfate transport ATP-binding protein 1452220 cysA 4608436 cysA Azoarcus sp. BH72 putative sulfate transport ATP-binding protein YP_932837.1 1451135 R 62928 CDS YP_932838.1 119897625 4608554 complement(1452232..1453119) 1 NC_008702.1 Sulfate transport system permease cysW. Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane. InterPro: Binding-protein-dependent transport systems inner membrane component uppS: undecaprenyl diphosphate synthase; High confidence in function and specificity; putative sulfate transport system permease 1453119 cysW 4608554 cysW Azoarcus sp. BH72 putative sulfate transport system permease YP_932838.1 1452232 R 62928 CDS YP_932839.1 119897626 4608566 complement(1453119..1453973) 1 NC_008702.1 Probable sulfate transport system permease cysT. Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane (By similarity). InterPro: Binding-protein-dependent transport systems inner membrane component gntP: gluconate transporter; High confidence in function and specificity; sulfate transporter permease 1453973 cysT 4608566 cysT Azoarcus sp. BH72 sulfate transporter permease YP_932839.1 1453119 R 62928 CDS YP_932840.1 119897627 4608565 complement(1453973..1454983) 1 NC_008702.1 CysP: sulfate/thiosulfate periplasmic binding protein. Sulfate-binding protein precursor (Sulfate starvation-induced protein 2) (SSI2). This protein specifically binds sulfate and is involved in its transmembrane transport. mazG: MazG family protein; High confidence in function and specificity; putative sulfate transport system substrate-binding protein 1454983 cysP 4608565 cysP Azoarcus sp. BH72 putative sulfate transport system substrate-binding protein YP_932840.1 1453973 R 62928 CDS YP_932841.1 119897628 4608563 1455298..1456512 1 NC_008702.1 Conserved hypothetical peptidoglycan binding protein. Homology to bb3579 of b. bronchiseptica of 69% (trembl|Q7WGL0) InterPro: Putative peptidoglycan binding domain 1 (IPR002477) Pfam: Putative peptidoglycan binding domain This domain is composed of three alpha helices. This domain is found at the N or C terminus of a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function. signal peptide no TMHs; Family membership; peptidoglycan-binding protein 1456512 4608563 azo1337 Azoarcus sp. BH72 peptidoglycan-binding protein YP_932841.1 1455298 D 62928 CDS YP_932842.1 119897629 4606825 1456606..1457373 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8FWK3 (58% identity); TREMBL:Q9AHY1 (58% identity). Pfam (PF00106): Short chain dehydrogenase.; Family membership; short-chain dehydrogenase 1457373 4606825 azo1338 Azoarcus sp. BH72 short-chain dehydrogenase YP_932842.1 1456606 D 62928 CDS YP_932843.1 119897630 4606826 1457532..1460015 1 NC_008702.1 36% ATPase-IB1_Cu.IPR006416; ATPase-IB_hvy.IPR001757; ATPase_E1-E2.IPR005834; Dehal_like_hydro.IPR008250; E1-E2_ATPase_reg. IPR006121; HeavyMe_transpt.IPR000695; H_ATPase.IPR006191; Metal_bind. Pfam:PF00122; E1-E2_ATPase; 1.PF00403; HMA; 1.PF00702; Hydrolase; 1. TMHs:8; Specificity unclear; putative cation-transporting ATPase 1460015 ccoI 4606826 ccoI Azoarcus sp. BH72 putative cation-transporting ATPase YP_932843.1 1457532 D 62928 CDS YP_932844.1 119897631 4608453 1460020..1460229 1 NC_008702.1 Conserved hypothetical cytochrome oxidase maturation protein. Homology to cco of P.Putida of 42% (gnl|keqq|ppu:PP4262(KEGG)). Pfam: Cytochrome oxidase maturation protein cbb3-type. Genes encoding a cytochrome cbb3 oxidase were initially designated fixNOQP (ccoNOQP),the ccoNOQP operon is always found close to a second gene cluster, known as fixGHIS (ccoGHIS) whose expression is necessary for the assembly of a functional cbb3 oxidase. On the basis of their derived amino acid sequences each of the four proteins encoded by the ccoGHIS operon are thought to be membrane-bound. It has been suggested that they may function in concert as a multi-subunit complex,possibly playing a role in the uptake and metabolism of copper required for the assembly of the binuclear centre of cytochrome cbb3 oxidase. Interpro: IPR004714 Cytochrome oxidase maturation protein cbb3-type. No signal peptide. 1 TMHs; Conserved hypothetical protein; cytochrome oxidase maturation protein 1460229 ccoS 4608453 ccoS Azoarcus sp. BH72 cytochrome oxidase maturation protein YP_932844.1 1460020 D 62928 CDS YP_932845.1 119897632 4608458 1460596..1462020 1 NC_008702.1 CcoN; FixN; cbb3-type cytochrome c oxidase subunit I 1462020 ccoN 4608458 ccoN Azoarcus sp. BH72 cbb3-type cytochrome c oxidase subunit I YP_932845.1 1460596 D 62928 CDS YP_932846.1 119897633 4608454 1462036..1462671 1 NC_008702.1 CcoO; FixO; cbb3-type cytochrome c oxidase subunit II 1462671 ccoO 4608454 ccoO Azoarcus sp. BH72 cbb3-type cytochrome c oxidase subunit II YP_932846.1 1462036 D 62928 CDS YP_932847.1 119897634 4608455 1462691..1462864 1 NC_008702.1 Cbb3-type cytochrome oxidase, subunit ccoQ , 44% Identity to TrEMBL;Q8D9I3, Q7MKV5,Q9KS21. Has PF05545,Cbb3-type cytochrome oxidase component FixQ; IPR008621;This family consists of several Cbb3-type cytochrome oxidase components (FixQ/CcoQ). FixQ is found in nitrogen fixing bacteria. Since nitrogen fixation is an energy-consuming process, effective symbioses depend on operation of a respiratory chain with a high affinity for O2, closely coupled to ATP production. This requirement is fulfilled by a special three-subunit terminal oxidase (cytochrome terminal oxidase cbb3), which was first identified in Bradyrhizobium japonicum as the product of the fixNOQP operon.; Cbb3-type cytochrome oxidase subunit 1462864 ccoQ 4608455 ccoQ Azoarcus sp. BH72 Cbb3-type cytochrome oxidase subunit YP_932847.1 1462691 D 62928 CDS YP_932848.1 119897635 4608457 1462868..1463761 1 NC_008702.1 Probable cytochrome C oxidase subunit III. Homology to ccoP of P. stutzeri of 46% (trembl|Q8KS19) CYTOCHROME C OXIDASE IS THE COMPONENT OF THE RESPIRATORY CHAIN THAT CATALYZES THE REDUCTION OF OXYGEN TO WATER. SUBUNITS 1- 3 FORM THE FUNCTIONAL CORE OF THE ENZYME COMPLEX. CO I IS THE CATALYTIC SUBUNIT OF THE ENZYME. ELECTRONS ORIGINATING IN CYTOCHROME C OR A QUINOL ARE TRANSFERRED TO THE BIMETALLIC CENTER FORMED BY A HIGH-SPIN HEME AND COPPER B. Tigrfam: ccoP: cytochrome c oxidase cbb3-type Pfam: cytochrome c no signal peptide probable 2 TMHs; High confidence in function and specificity; cytochrome c oxidase, cbb3-type subunit III 1463761 ccoP 4608457 ccoP Azoarcus sp. BH72 cytochrome c oxidase, cbb3-type subunit III YP_932848.1 1462868 D 62928 CDS YP_932849.1 119897636 4608456 1463836..1465260 1 NC_008702.1 Putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein. Homology to fixG of R. meliloti of 36% (FIXG_RHIME). Ferredoxins are iron-sulphur proteins that mediate electron transfer in a range of metabolic reactions; they fall into several subgroups according to the nature of their iron-sulphur cluster(s). One group,originally found in bacteria, has been termed ''bacterial-type'', in which the active centre is a 4Fe-4S cluster. 4Fe-4S ferredoxins may in turn be subdivided into further groups, based on their sequence properties. Most contain at least one conserved domain, including four Cys residues that bind to a 4Fe-4S centre. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450) probable 5 TMHs . No signal peptide predicted.; Family membership; putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein 1465260 ccoG 4608456 ccoG Azoarcus sp. BH72 putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein YP_932849.1 1463836 D 62928 CDS YP_932850.1 119897637 4608451 1465265..1465780 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA5136 Azoarcus sp. EbN1 of 51% (gnl|keqq|eba:ebA5136(KEGG)). Has PF05751, FixH;IPR008620; This family consists of several Rhizobium FixH like proteins. It has been suggested that suggested that the four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalysed by FixG. No signal peptide predicted. 1 TMHs; Conserved hypothetical protein; hypothetical protein 1465780 ccoH 4608451 ccoH Azoarcus sp. BH72 hypothetical protein YP_932850.1 1465265 D 62928 CDS YP_932851.1 119897638 4608452 1465806..1465955 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domain predicted. No signal peptide 1 TMH; hypothetical protein 1465955 4608452 azo1347 Azoarcus sp. BH72 hypothetical protein YP_932851.1 1465806 D 62928 CDS YP_932852.1 119897639 4606827 1465986..1466222 1 NC_008702.1 Probable transmemebrane Protein, 38% Idneity to TrEMBL;Q8XZW3 Signal P reporting Signal peptide present. TMHMM2 reporting 1 TMH present.; transmembrane protein 1466222 4606827 azo1348 Azoarcus sp. BH72 transmembrane protein YP_932852.1 1465986 D 62928 CDS YP_932853.1 119897640 4609982 complement(1466484..1466927) 1 NC_008702.1 Putative universal stress protein F,; Conserved hypothetical protein; putative universal stress protein f 1466927 4609982 azo1349 Azoarcus sp. BH72 putative universal stress protein f YP_932853.1 1466484 R 62928 CDS YP_932854.1 119897641 4606829 1467151..1468884 1 NC_008702.1 FUNCTION: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation. Entry name :-PHBC_AZOCA Primary accession number:- O66392 Identity:-41% InterPro :- IPR000073; A/b_hydrolase. Pfam:- PF07167; PhaC_N; 1. Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; poly-beta-hydroxybutyrate synthase 1468884 phbC2 4606829 phbC2 Azoarcus sp. BH72 poly-beta-hydroxybutyrate synthase YP_932854.1 1467151 D 62928 CDS YP_932855.1 119897642 4609542 complement(1468907..1469707) 1 NC_008702.1 Fumarate and nitrate reduction regulatory protein. Global transcription factor that controls the expression of over 100 target genes in response to anoxia. It facilitates the adaptation to anaerobic growth conditions by regulating the expression of gene products that are involved in anaerobic energy metabolism. When the terminal electron acceptor O(2) is no longer available it represses the synthesis of enzymes involved in aerobic respiration and increases the synthesis of enzymes required for anaerobic respiration. Similar to SWISSPROT: sprot|FNR_ECOLI (39% Escherichia coli, fumarate and nitrate reduction regulatory protein Fnr) / sprot|ANR_PSEAE (40% Pseudomonas aeruginosa,transcriptional activator protein Anr) InterPro: IPR000595 cNMP_binding. IPR001808 HTH_Crp. Pfam: PF00027 cNMP_binding. PF00325 Crp. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; fumarate and nitrate reduction regulatory protein 1469707 fnr 4609542 fnr Azoarcus sp. BH72 fumarate and nitrate reduction regulatory protein YP_932855.1 1468907 R 62928 CDS YP_932856.1 119897643 4608816 1469756..1471147 1 NC_008702.1 catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III; coproporphyrinogen III oxidase 1471147 hemN 4608816 hemN Azoarcus sp. BH72 coproporphyrinogen III oxidase YP_932856.1 1469756 D 62928 CDS YP_932857.1 119897644 4608988 1471235..1471951 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA5153 of Azoarcus sp. EbN1 of 79% (gnl|keqq|eba:ebA5153(KEGG)). Has PF02683;Cytochrome C biogenesis protein transmembrane region (IPR003834 Ctytoch_TM): This family consists of the transmembrane (i.e. non-catalytic) region of Cytochrome C biogenesis proteins also known as disulphide interchange proteins. These proteins posses a protein disulphide isomerase like domain that is not found within the aligned region of this family. No signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 1471951 4608988 azo1353 Azoarcus sp. BH72 hypothetical protein YP_932857.1 1471235 D 62928 CDS YP_932858.1 119897645 4606830 complement(1471968..1472363) 1 NC_008702.1 Heavy metal dependent transcription regulator 2. TRANSCRIPTIONAL REGULATOR INVOLVED IN ACID TOLERANCE. BINDS COPPER (By similarity). It contains a n-terminal dna binding region and a c- terminal metal binding region (by similarity). 35% HTH_MerR.IPR009061; Putativ_DNA_bind. Pfam:PF00376; MerR; 1. TIGR00372: conserved hypothetical protei; High confidence in function and specificity; putative regulatory protein 1472363 copY 4606830 copY Azoarcus sp. BH72 putative regulatory protein YP_932858.1 1471968 R 62928 CDS YP_932859.1 119897646 4608516 1472442..1474877 1 NC_008702.1 CopA: copper transporting P-type ATPase,involved in the uptake and metabolism of copper. 44% ATPase-IB1_Cu.IPR006416; ATPase-IB_hvy.IPR001757; ATPase_E1-E2.IPR001756; Cu_ATPase.IPR005834; Dehal_like_hydro.IPR008250; E1-E2_ATPase_reg.IPR006121; HeavyMe_transpt.IPR006191; Metal_bind. Pfam:PF00122; E1-E2_ATPase; 1.PF00403; HMA; 1.PF00702; Hydrolase; 1. TIGR00003: copper-ion-binding protein. TMHs:8.; High confidence in function and specificity; putative Cu2+ transporting ATPase 1474877 copA 4608516 copA Azoarcus sp. BH72 putative Cu2+ transporting ATPase YP_932859.1 1472442 D 62928 CDS YP_932860.1 119897647 4608513 1474902..1475111 1 NC_008702.1 Conserved hypothetical copper chaperon. Homology to copZ of copZ Azoarcus sp. EbN1 of 66%. Pfam: Heavy-metal-associated domain. Proteins that transport heavy metals in micro-organisms and mammals share similarities in their sequences and structures. Tigrfam: TIGR00003: copper-ion-binding protein. No signal peptide. No TMHs.; Conserved hypothetical protein; copper chaperon 1475111 copZ 4608513 copZ Azoarcus sp. BH72 copper chaperon YP_932860.1 1474902 D 62928 CDS YP_932861.1 119897648 4608517 complement(1475126..1477969) 1 NC_008702.1 EAL/GGDEF/PAS/PAC-domain containing signalling protein,; Conserved hypothetical protein; EAL/GGDEF/PAS/PAC-domain-containing signalling protein 1477969 4608517 azo1357 Azoarcus sp. BH72 EAL/GGDEF/PAS/PAC-domain-containing signalling protein YP_932861.1 1475126 R 62928 CDS YP_932862.1 119897649 4606831 1478241..1478768 1 NC_008702.1 Probable inorganic pyrophosphatase. Homology to ppa of E. coli of 66% (sprot|IPYR_ECOLI). CATALYTIC ACTIVITY Diphosphate + H(2)O = 2 phosphate. Pfam: Inorganic pyrophospatease no signal peptide no TMHs; High confidence in function and specificity; inorganic pyrophosphatase 1478768 ppa 4606831 ppa Azoarcus sp. BH72 inorganic pyrophosphatase YP_932862.1 1478241 D 62928 CDS YP_932863.1 119897650 4609607 complement(1478841..1479977) 1 NC_008702.1 Conserved hypothetical protein. Homology to BB3581 of B.bronchiseptica of 53% (tremble:Q7WGK8). Has PF04339:(IPR00743)Protein of unknown function, DUF482;This family contains several proteins of uncharacterised function. NO signal peptide or TMH present.; hypothetical protein 1479977 4609607 azo1359 Azoarcus sp. BH72 hypothetical protein YP_932863.1 1478841 R 62928 CDS YP_932864.1 119897651 4606832 1480139..1481755 1 NC_008702.1 NH(3)-dependent; catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NAAD) using either ammonia or glutamine as the amide donor and ATP; ammonia-utilizing enzymes include the ones from Bacillus and Escherichia coli while glutamine-utilizing enzymes include the Mycobacterial one; forms homodimers; NAD synthetase 1481755 nadE 4606832 nadE Azoarcus sp. BH72 NAD synthetase YP_932864.1 1480139 D 62928 CDS YP_932865.1 119897652 4609306 1481805..1482143 1 NC_008702.1 PII-like signal transmitter proteins are involved in the regulation of ammonium assimilation and nitrogen fixation. The PII-like proteins differed from each other in details of N-sensing. They were covalently modified by uridylylation upon nitrogen limitation. Similar to trembl|Q9EZQ2 (100%) and to trembl|Q8XWX5 (82%). Pfam: Nitrogen regulatory protein P-II; High confidence in function and specificity; PII-like signal transmitter protein GlnB 1482143 glnB 4609306 glnB Azoarcus sp. BH72 PII-like signal transmitter protein GlnB YP_932865.1 1481805 D 62928 CDS YP_932866.1 119897653 4608895 complement(1482173..1483225) 1 NC_008702.1 Hypothetical protein. no homology of the entire protein with the data bank. Pfam: Smr domain InterPro: Smr domain This family includes the Smr (Small MutS Related) proteins, and the C-terminal region of the MutS2 protein. It has been suggested that this domain interacts with the MutS1 protein in the case of Smr proteins and with the N-terminal MutS related region of MutS2. no signal peptide no TMHs; hypothetical protein 1483225 4608895 azo1362 Azoarcus sp. BH72 hypothetical protein YP_932866.1 1482173 R 62928 CDS YP_932867.1 119897654 4606833 complement(1483241..1484191) 1 NC_008702.1 Thioredoxin-disulfide reductase(EC 1.8.1.9). Homology to trxB of E. coli of 71% (sprot|TRXB_ECOLI). Catalyse the reaction: thioredoxin + nadp(+) = thioredoxin disulfide + nadph Pfam: Pyridine nucleotide-disulphide oxidoreductase signal peptide no TMHs; High confidence in function and specificity; thioredoxin-disulfide reductase 1484191 trxB2 4606833 trxB2 Azoarcus sp. BH72 thioredoxin-disulfide reductase YP_932867.1 1483241 R 62928 CDS YP_932868.1 119897655 4610042 1484423..1485094 1 NC_008702.1 Probable cyclic amp receptor-like protein,; High confidence in function and specificity; cyclic AMP receptor protein 1485094 vfr 4610042 vfr Azoarcus sp. BH72 cyclic AMP receptor protein YP_932868.1 1484423 D 62928 CDS YP_932869.1 119897656 4610092 1485243..1487531 1 NC_008702.1 DNA translocase ftsK 2. InterPro: FtsK/SpoIIIE family; High confidence in function and specificity; putative cell division protein 1487531 FtsK 4610092 FtsK Azoarcus sp. BH72 putative cell division protein YP_932869.1 1485243 D 62928 CDS YP_932870.1 119897657 4608837 1487528..1488205 1 NC_008702.1 Putative outer-membrane lipoprotein carrier protein. Homology to lolA of E. coli of 25% (sprot|LOLA_ECOLI) Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane) (By similarity). Pfam: outer membrane lipoprotein carrier protein LolA Tigrfam: lolA: outer membrane lipoprotein carrier singal peptide no TMHs; Family membership; putative outer membrane lipoprotein carrier protein 1488205 lolA 4608837 lolA Azoarcus sp. BH72 putative outer membrane lipoprotein carrier protein YP_932870.1 1487528 D 62928 CDS YP_932871.1 119897658 4609168 1488217..1489554 1 NC_008702.1 Hypothetical protein CBU1189. InterPro: AAA-protein (ATPases associated with various cellular activities) ruvB: Holliday junction DNA helicase R.; Specificity unclear; recombination factor protein RarA 1489554 4609168 azo1367 Azoarcus sp. BH72 recombination factor protein RarA YP_932871.1 1488217 D 62928 CDS YP_932872.1 119897659 4606834 complement(1489593..1489856) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA7035 of Azoarcus sp. EbN1 of 79% (gnl|keqq|eba:ebA7035(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1489856 4606834 azo1368 Azoarcus sp. BH72 hypothetical protein YP_932872.1 1489593 R 62928 CDS YP_932873.1 119897660 4606835 1490125..1491417 1 NC_008702.1 catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; seryl-tRNA synthetase 1491417 4606835 azo1369 Azoarcus sp. BH72 seryl-tRNA synthetase YP_932873.1 1490125 D 62928 CDS YP_932874.1 119897661 4606836 1491592..1493208 1 NC_008702.1 Aerotaxis receptor protein,; Specificity unclear; putative aerotaxis receptor protein 1493208 4606836 azo1370 Azoarcus sp. BH72 putative aerotaxis receptor protein YP_932874.1 1491592 D 62928 CDS YP_932875.1 119897662 4606837 complement(1493285..1495105) 1 NC_008702.1 Dihydrolipoamide dehydrogenase. Homology to lpdA of N. meningitides of 70% (trembl|Q59099). THE BRANCHED-CHAIN ALPHA-KETO DEHYDROGENASE COMPLEX CATALYZES THE OVERALL CONVERSION OF ALPHA-KETO ACIDS TO ACYL-COA AND CO(2). IT CONTAINS MULTIPLE COPIES OF 3 ENZYMATIC COMPONENTS: BRANCHED-CHAIN ALPHA-KETO ACID DECARBOXYLASE (E1) LIPOAMIDE ACYLTRANSFERASE (E2) AND LIPOAMIDE DEHYDROGENASE (E3) (BY SIMILARITY). InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327); Pyridine nucleotide-disulphide oxidoreductase, classI (IPR001100); biotin/Lipoyl attachment (IPR000089); Pyridine nucleotide-disulphide oxidoreductase dimersiation domain (IPR004099); NAD binding site (IPR000205); 2-oxo acid dehydrogenase acetyltransferase, classI (IPR003016) Pfam: Biotin-requiring enzyme; Pyridine nucleotide-disulphide oxidoreductase; Pyridine nucleotide oxidoreductase dimersation domain no TMHs no signal peptide; High confidence in function and specificity; dihydrolipoamide dehydrogenase 1495105 lpdA 4606837 lpdA Azoarcus sp. BH72 dihydrolipoamide dehydrogenase YP_932875.1 1493285 R 62928 CDS YP_932876.1 119897663 4609173 complement(1495116..1496804) 1 NC_008702.1 Probable dihydrolipoamide acetyltransferase. Homology to pdhB of A. eutrophus of 62% (sprot|ODP2_ALCEU) THE PYRUVATE DEHYDROGENASE COMPLEX CATALYZES THE OVERALL CONVERSION OF PYRUVATE TO ACETYL-COA & CO(2). IT CONTAINS MULTIPLE COPIES OF THREE ENZYMATIC COMPONENTS: PYRUVATE DEHYDROGENASE (E1) DIHYDROLIPOAMIDE ACETYLTRANSFERASE (E2) & LIPOAMIDE DEHYDROGENASE (E3) (BY SIMILARITY). InterPro: 2-Oxo acid dehydrogenase acyltransferase catalytic domain (IPR001078); Type I antifreeze protein (IPR000104); Biotin/Lipoyl attachment (IPR000089); E3 binding domain (IPR004167); 2-Oxo acid dehydrogenase acyltransferase component lipoyl binding site (IPR003016) Pfam: Biotin-requiring enzyme; E3 binding domain; 2-oxo dehydrogenase acyltransferase Tigrfam: BCCP: acetyl-CoA carboxylase biotin carboxyl carrier protein no TMHs no signal peptide; High confidence in function and specificity; dihydrolipoamide acetyltransferase 1496804 pdhB 4609173 pdhB Azoarcus sp. BH72 dihydrolipoamide acetyltransferase YP_932876.1 1495116 R 62928 CDS YP_932877.1 119897664 4609514 complement(1496825..1499506) 1 NC_008702.1 E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC; pyruvate dehydrogenase subunit E1 1499506 aceE 4609514 aceE Azoarcus sp. BH72 pyruvate dehydrogenase subunit E1 YP_932877.1 1496825 R 62928 CDS YP_932878.1 119897665 4609513 complement(1499913..1501118) 1 NC_008702.1 This family includes extracellular ligand binding domains of a wide range of receptors and it also includes the bacterial amino acid binding proteins of known structure. Similar to tremblnew|AAR35111 (24%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Family membership; extracellular ligand binding protein 1501118 4609513 azo1374 Azoarcus sp. BH72 extracellular ligand binding protein YP_932878.1 1499913 R 62928 CDS YP_932879.1 119897666 4606838 1501285..1503999 1 NC_008702.1 Putative two-component sensor kinase,; Specificity unclear; putative two-component sensor kinase 1503999 4606838 azo1375 Azoarcus sp. BH72 putative two-component sensor kinase YP_932879.1 1501285 D 62928 CDS YP_932880.1 119897667 4606839 1503974..1504612 1 NC_008702.1 Probable transcriptional regulatory protein,; Specificity unclear; transcriptional regulator 1504612 fixJ2 4606839 fixJ2 Azoarcus sp. BH72 transcriptional regulator YP_932880.1 1503974 D 62928 CDS YP_932881.1 119897668 4608767 1504697..1505572 1 NC_008702.1 catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate; bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase 1505572 folD 4608767 folD Azoarcus sp. BH72 bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase YP_932881.1 1504697 D 62928 CDS YP_932882.1 119897669 4608820 1505580..1506092 1 NC_008702.1 Phosphoribosylaminoimidazole carboxylase(AIR carboxylase) (AIRC). InterPro: 1-(5-Phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase; High confidence in function and specificity; phosphoribosylaminoimidazole carboxylase 1506092 purE 4608820 purE Azoarcus sp. BH72 phosphoribosylaminoimidazole carboxylase YP_932882.1 1505580 D 62928 CDS YP_932883.1 119897670 4609658 1506220..1507401 1 NC_008702.1 With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway; phosphoribosylaminoimidazole carboxylase ATPase subunit 1507401 purK 4609658 purK Azoarcus sp. BH72 phosphoribosylaminoimidazole carboxylase ATPase subunit YP_932883.1 1506220 D 62928 CDS YP_932884.1 119897671 4609661 1507398..1508429 1 NC_008702.1 Hypothetical protein ywlC. TIGR00057: Sua5/YciO/YrdC/YwlC family; Specificity unclear; hypothetical protein 1508429 4609661 azo1380 Azoarcus sp. BH72 hypothetical protein YP_932884.1 1507398 D 62928 CDS YP_932885.1 119897672 4609979 1508792..1509934 1 NC_008702.1 catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers; carbamoyl phosphate synthase small subunit 1509934 carA 4609979 carA Azoarcus sp. BH72 carbamoyl phosphate synthase small subunit YP_932885.1 1508792 D 62928 CDS YP_932886.1 119897673 4608414 1509989..1513216 1 NC_008702.1 four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity; carbamoyl phosphate synthase large subunit 1513216 carB 4608414 carB Azoarcus sp. BH72 carbamoyl phosphate synthase large subunit YP_932886.1 1509989 D 62928 CDS YP_932887.1 119897674 4608415 1513213..1513689 1 NC_008702.1 Transcription elongation factor GreA Transcription elongation factor greA (Transcript cleavage factor greA). Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as greA or greB allows the resumption of elongation from the new 3terminus. GreA releases sequences of 2 to 3 nucleotides (By similarity). Belongs to the greA/greB family. 58% 1. PF03449 GreA_GreB_N; 1. TIGFRAM TIGR01462; greA; 1. PurN: phosphoribosylglycinamide formylt; High confidence in function and specificity; putative transcription elongation factor 1513689 greA 4608415 greA Azoarcus sp. BH72 putative transcription elongation factor YP_932887.1 1513213 D 62928 CDS YP_932888.1 119897675 4608935 1513701..1514171 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS03781 of Ralstonia solanacearum of 39% (trembl|Q8XZ81(SRS)). no domains predicted. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 1514171 4608935 azo1384 Azoarcus sp. BH72 hypothetical protein YP_932888.1 1513701 D 62928 CDS YP_932889.1 119897676 4606841 complement(1514202..1514663) 1 NC_008702.1 hypothetical protein 1514663 4606841 azo1385 Azoarcus sp. BH72 hypothetical protein YP_932889.1 1514202 R 62928 CDS YP_932890.1 119897677 4606842 1514707..1515324 1 NC_008702.1 Ribosomal RNA large subunit methyltransferase J (EC 2.1.1.-) (rRNA (uridine-2-O-)-methyltransferase) (23S rRNA m2U2552 methyltransferase) (Cell division protein ftsJ homolog). Specifically methylates the uridine in position 2552 of 23S rRNA in the 50S particle; High confidence in function and specificity; ribosomal RNA large subunit methyltransferase J 1515324 FtsJ 4606842 FtsJ Azoarcus sp. BH72 ribosomal RNA large subunit methyltransferase J YP_932890.1 1514707 D 62928 CDS YP_932891.1 119897678 4608836 1515440..1517332 1 NC_008702.1 Cell division protease ftsH. Homology to ftsH of E. coli of 64% (SWISSPROT:FTSH_ECOLI) SEEMS TO ACT AS AN ATP-DEPENDENT ZINC METALLOPEPTIDASE. INVOLVED IN THE DEGRADATION OF SIGMA-32. DEGRADES CARBOXY- TERMINAL-TAGGED CYTOPLASMIC PROTEINS. THESE PROTEINS ARE TAGGED WITH AN 11-AMINO-ACID NONPOLAR DESTABILIZING TAIL VIA A MECHANISM INVOLVING THE 10SA (SSRA) STABLE RNA (By similarity). Pfam: ATPase family associated with various celluar activities;Peptidase family M41 probable signal peptide probable 1 TMH; High confidence in function and specificity; cell division protein FtsH 1517332 FtsH 4608836 FtsH Azoarcus sp. BH72 cell division protein FtsH YP_932891.1 1515440 D 62928 CDS YP_932892.1 119897679 4608834 1517394..1518215 1 NC_008702.1 Dihydropteroate synthase (EC 2.5.1.15) (DHPS) (Dihydropteroate pyrophosphorylase). DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide (By similarity).; High confidence in function and specificity; dihydropteroate synthase 1518215 folP 4608834 folP Azoarcus sp. BH72 dihydropteroate synthase YP_932892.1 1517394 D 62928 CDS YP_932893.1 119897680 4608823 1518249..1519604 1 NC_008702.1 catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate; phosphoglucosamine mutase 1519604 glmM 4608823 glmM Azoarcus sp. BH72 phosphoglucosamine mutase YP_932893.1 1518249 D 62928 CDS YP_932894.1 119897681 4608891 1519717..1520757 1 NC_008702.1 61% Peri-phosph.IPR006059; SBP_bac_1. Pfam:PF01547; SBP_bac_1; 1. TIGRFAMs:TIGR00975; 3a0107s03; 1. Signal peptide present.; High confidence in function and specificity; phosphate-binding periplasmic protein 1520757 pstS2 4608891 pstS2 Azoarcus sp. BH72 phosphate-binding periplasmic protein YP_932894.1 1519717 D 62928 CDS YP_932895.1 119897682 4609644 1520867..1521829 1 NC_008702.1 part of the ATP-dependent phosphate uptake system PstABCS responsible for inorganic phosphate (Pi) uptake under Pi starvation conditions; phosphate transporter permease subunit PstC 1521829 pstC 4609644 pstC Azoarcus sp. BH72 phosphate transporter permease subunit PstC YP_932895.1 1520867 D 62928 CDS YP_932896.1 119897683 4609642 1521902..1522681 1 NC_008702.1 Region start changed from 1521840 to 1521903 (-63 bases); phosphate ABC transporter permease 1522681 4609642 azo1392 Azoarcus sp. BH72 phosphate ABC transporter permease YP_932896.1 1521902 D 62928 CDS YP_932897.1 119897684 4606843 1522714..1523478 1 NC_008702.1 Phosphate import ATP-binding protein pstB (EC 3.6.3.27) (Phosphate- transporting ATPase) (ABC phosphate transporter) (Peripheral membrane protein B). Part of the ABC transporter complex pstSACB (TC 3.A.1.7.1) involved in phosphate import. Responsible for energy coupling to the transport system. mobB: molybdopterin-guanine dinucleotide; High confidence in function and specificity; phosphate ABC transporter ATP-binding protein 1523478 pstB 4606843 pstB Azoarcus sp. BH72 phosphate ABC transporter ATP-binding protein YP_932897.1 1522714 D 62928 CDS YP_932898.1 119897685 4609641 1523592..1524320 1 NC_008702.1 Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate; triosephosphate isomerase 1524320 tpiA 4609641 tpiA Azoarcus sp. BH72 triosephosphate isomerase YP_932898.1 1523592 D 62928 CDS YP_932899.1 119897686 4609949 1524363..1524725 1 NC_008702.1 Protein-export membrane protein secG. Involved in protein export. Participates in an early event of protein translocation (By similarity). Together with SecY and SecG, SecE forms a multimeric channel through which preproteins are translocated, using both proton motive forces and ATP-driven secretion. pfam: preprotein translocase tigerfam: preprotein translocase most propable TMH: 2 no signal peptide InterPro: Preprotein translocase SecG subunit Pfam: secG: preprotein translocase SecG subuni; High confidence in function and specificity; preprotein translocase subunit SecG 1524725 secG 4609949 secG Azoarcus sp. BH72 preprotein translocase subunit SecG YP_932899.1 1524363 D 62928 CDS YP_932900.1 119897687 4610005 1524978..1525352 1 NC_008702.1 NADH-ubiquinone oxidoreductase chain 3 (EC 1.6.5.3). Ndh-1 shuttles electrons from nadh, via fmn and iron- sulfur (fe-s) centers, to quinones in the respiratory chain. the immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (by similarity).; High confidence in function and specificity; putative NADH-ubiquinone oxidoreductase subunit A 1525352 nuoA 4610005 nuoA Azoarcus sp. BH72 putative NADH-ubiquinone oxidoreductase subunit A YP_932900.1 1524978 D 62928 CDS YP_932901.1 119897688 4609418 1525357..1525833 1 NC_008702.1 The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen; NADH dehydrogenase subunit B 1525833 nuoB2 4609418 nuoB2 Azoarcus sp. BH72 NADH dehydrogenase subunit B YP_932901.1 1525357 D 62928 CDS YP_932902.1 119897689 4609420 1525851..1526456 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit C 1526456 nuoC 4609420 nuoC Azoarcus sp. BH72 NADH dehydrogenase subunit C YP_932902.1 1525851 D 62928 CDS YP_932903.1 119897690 4609421 1526449..1527702 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit D 1527702 nuoD 4609421 nuoD Azoarcus sp. BH72 NADH dehydrogenase subunit D YP_932903.1 1526449 D 62928 CDS YP_932904.1 119897691 4609422 1527783..1528262 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit E 1528262 nuoE 4609422 nuoE Azoarcus sp. BH72 NADH dehydrogenase subunit E YP_932904.1 1527783 D 62928 CDS YP_932905.1 119897692 4609423 1528259..1529578 1 NC_008702.1 NADH-quinone oxidoreductase chain F (EC 1.6.99.5) (NADH dehydrogenase I chain F) (NDH-1 chain F). NDH-1 shuttles electrons from NADH via FMN and iron- sulfur (Fe-S) centers to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred four hydrogen ions are translocated across the cytoplasmic membrane) and thus conserves the redox energy in a proton gradient. InterPro: Respiratory-chain NADH dehydrogenase 51 Kd subunit gnd_rel: 6-phosphogluconate dehydrogenas; High confidence in function and specificity; putative NADH-ubiquinone oxidoreductase subunit F 1529578 nuoF 4609423 nuoF Azoarcus sp. BH72 putative NADH-ubiquinone oxidoreductase subunit F YP_932905.1 1528259 D 62928 CDS YP_932906.1 119897693 4609424 1529572..1531887 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to ubiquinone; NADH dehydrogenase subunit G 1531887 nuoG 4609424 nuoG Azoarcus sp. BH72 NADH dehydrogenase subunit G YP_932906.1 1529572 D 62928 CDS YP_932907.1 119897694 4609425 1531887..1532936 1 NC_008702.1 NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH dehydrogenase I chain H) (NDH-1 chain H). NDH-1 shuttles electrons from NADH via FMN and iron- sulfur (Fe-S) centers to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred four hydrogen ions are translocated across the cytoplasmic membrane) and thus conserves the redox energy in a proton gradient (By similarity). InterPro: Respiratory-chain NADH dehydrogenase subunit 1 nup: nucleoside transporter; High confidence in function and specificity; putative NADH-ubiquinone oxidoreductase subunit H 1532936 nuoH 4609425 nuoH Azoarcus sp. BH72 putative NADH-ubiquinone oxidoreductase subunit H YP_932907.1 1531887 D 62928 CDS YP_932908.1 119897695 4609426 1532947..1533432 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit I 1533432 nuoI 4609426 nuoI Azoarcus sp. BH72 NADH dehydrogenase subunit I YP_932908.1 1532947 D 62928 CDS YP_932909.1 119897696 4609427 1533469..1534071 1 NC_008702.1 NADH-quinone oxidoreductase chain J (EC 1.6.99.5) (NADH dehydrogenase I chain J) (NDH-1 chain J). NDH-1 shuttles electrons from NADH via FMN and iron- sulfur (Fe-S) centers to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred four hydrogen ions are translocated across the cytoplasmic membrane) and thus conserves the redox energy in a proton gradient. MIP: MIP family channel proteins; High confidence in function and specificity; NADH-ubiquinone oxidoreductase subunit J 1534071 nuoJ 4609427 nuoJ Azoarcus sp. BH72 NADH-ubiquinone oxidoreductase subunit J YP_932909.1 1533469 D 62928 CDS YP_932910.1 119897697 4609428 1534128..1534433 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit K 1534433 nuoK 4609428 nuoK Azoarcus sp. BH72 NADH dehydrogenase subunit K YP_932910.1 1534128 D 62928 CDS YP_932911.1 119897698 4609429 1534446..1536470 1 NC_008702.1 NADH-quinone oxidoreductase chain L (EC 1.6.99.5) (NADH dehydrogenase I chain L) (NDH-1 chain L). NDH-1 shuttles electrons from NADH via FMN and iron- sulfur (Fe-S) centers to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred four hydrogen ions are translocated across the cytoplasmic membrane) and thus conserves the redox energy in a proton gradient (By similarity). 2A0310: amino acid permease (yeast); High confidence in function and specificity; NADH-ubiquinone oxidoreductase subunit L 1536470 nuoL 4609429 nuoL Azoarcus sp. BH72 NADH-ubiquinone oxidoreductase subunit L YP_932911.1 1534446 D 62928 CDS YP_932912.1 119897699 4609430 1536546..1538027 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit M 1538027 nuoM 4609430 nuoM Azoarcus sp. BH72 NADH dehydrogenase subunit M YP_932912.1 1536546 D 62928 CDS YP_932913.1 119897700 4609431 1538065..1539540 1 NC_008702.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit N 1539540 nuoN 4609431 nuoN Azoarcus sp. BH72 NADH dehydrogenase subunit N YP_932913.1 1538065 D 62928 CDS YP_932914.1 119897701 4609432 1539628..1540179 1 NC_008702.1 ADPribose diphosphatase. ACTIVE ON ADENOSINE(5)TRIPHOSPHO(5)ADENOSINE (AP3A) ADP-RIBOSE NADH ADENOSINE(5)DIPHOSPHO(5)ADENOSINE (AP2A). InterPro: NUDIX hydrolase TIGR00052: conserved hypothetical protein.; High confidence in function and specificity; ADP-ribose diphosphatase 1540179 4609432 azo1410 Azoarcus sp. BH72 ADP-ribose diphosphatase YP_932914.1 1539628 D 62928 CDS YP_932915.1 119897702 4606844 complement(1540276..1543152) 1 NC_008702.1 Conserved hypothetical ribonucleoside reductase. Homology to nrdE of C. violaceum of 62% (trembl|Q7NY29). Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides precursors that are necessary for DNA synthesis (By similarity). InterPro: Ribonucleotide reductase large subunit (IPR000788) Pfam: Ribonucleotide reductase, all-alpha domein; Ribonucleotide reductase, barrel domain no signal peptide no TMHs; High confidence in function and specificity; putative ribonucleoside reductase 1543152 4606844 azo1411 Azoarcus sp. BH72 putative ribonucleoside reductase YP_932915.1 1540276 R 62928 CDS YP_932916.1 119897703 4606845 1543481..1545289 1 NC_008702.1 Probable hydrogen dehydrogenase, alpha subunit. Homology to hoxF of A. eutrophus of 41% (sprot|HOXF_ALCEU). SUBUNITS ALPHA AND GAMMA OF HOXS CONSTITUTE AN NADH-OXIDOREDUCTASE. InterPro: Respiratory-chain NADH dehydrogenase 51 Kd subunit (IPR001949); Respiratory-chain NADH dehydrogenase 24 Kd subunit (IPR002023) Pfam: Respiratory-chain NADH dehydrogenase 24 Kd subunit; Respiratory-chain NADH dehydrogenase 51 Kd subunit no signal peptide no TMHs; High confidence in function and specificity; hydrogen dehydrogenase subunit alpha 1545289 hoxF 4606845 hoxF Azoarcus sp. BH72 hydrogen dehydrogenase subunit alpha YP_932916.1 1543481 D 62928 CDS YP_932917.1 119897704 4609019 1545286..1545990 1 NC_008702.1 Probable hydrogen dehydrogenase, gamma subunit. Homology to hoxU of A. eutrophus of 40% (pir|B35385(PIR). Binds 3 4Fe-4S clusters per subunit (Potential). Pfam: 2Fe-2S iron-sulfur cluster binding domain no signal peptide no TMHs; High confidence in function and specificity; hydrogen dehydrogenase subunit gamma 1545990 hoxU 4609019 hoxU Azoarcus sp. BH72 hydrogen dehydrogenase subunit gamma YP_932917.1 1545286 D 62928 CDS YP_932918.1 119897705 4609022 1545987..1546559 1 NC_008702.1 Probable hydrogen dehydrogenase, delta subunit. Homology to hoxY of a. eutrophus of 45% (sprot|HOXY_ALCEU). InterPro: Respiratory-chain NADH dehydrogenase 20 Kd subunit Pfam: NADH ubiquinone oxidoreductase, 20 kD subunit no signal peptie no TMHs; High confidence in function and specificity; hydrogen dehydrogenase subunit delta 1546559 hoxY 4609022 hoxY Azoarcus sp. BH72 hydrogen dehydrogenase subunit delta YP_932918.1 1545987 D 62928 CDS YP_932919.1 119897706 4609023 1546567..1548030 1 NC_008702.1 Probable hydrogen dehydrogenase, beta subunit. Homology to hoxH of a. eutrophus of 48% (sprot|HOXH_ALCEU). Pfam: Nickel-dependent hydrogenase no signal peptide no TMHs; High confidence in function and specificity; hydrogen dehydrogenase subunit beta 1548030 hoxH 4609023 hoxH Azoarcus sp. BH72 hydrogen dehydrogenase subunit beta YP_932919.1 1546567 D 62928 CDS YP_932920.1 119897707 4609020 1548062..1548553 1 NC_008702.1 conserved hypothetical protein. Homology to an orf of R. capsulata of 42% (trembl|Q9XBW7). Tigrfam: hycI: hydrogenase maturation protease no signal peptide no TMHs; hypothetical protein 1548553 4609020 azo1416 Azoarcus sp. BH72 hypothetical protein YP_932920.1 1548062 D 62928 CDS YP_932921.1 119897708 4606846 complement(1548563..1549420) 1 NC_008702.1 Conserved hypothetical protein. Homology to GSU0904 of Geobacter sulfurreducens of 31% (gnl|keqq|gsu:GSU0904(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1549420 4606846 azo1417 Azoarcus sp. BH72 hypothetical protein YP_932921.1 1548563 R 62928 CDS YP_932922.1 119897709 4606847 complement(1549449..1550681) 1 NC_008702.1 CzcD: Members of this family are integral membrane proteins that are found to increase tolerance to divalent metal ions such as cadmium, zinc, and cobalt. These proteins are thought to be efflux pumps that remove these ions from cells. 41% Cation_efflux. Pfam:PF01545; Cation_efflux; 1. TMhelix:6.; High confidence in function and specificity; putative cobalt-zinc-cadmium resistance protein 1550681 czcD2 4606847 czcD2 Azoarcus sp. BH72 putative cobalt-zinc-cadmium resistance protein YP_932922.1 1549449 R 62928 CDS YP_932923.1 119897710 4608574 1550659..1551510 1 NC_008702.1 Conserved hypothetical protein. Homology to Rsc1208 of R. solanacearum of 45% (trembl|Q8Y036). InterPro: AP endonucleases family 1 (IPR000097) tigrfam: xth: exodeoxyribonuclease III (xth) All proteins in this family for which functions are known are 5' AP endonucleases that function in base excision repair and the repair of abasic sites in DNA. Pfam: AP endonuclease family 1 no signal peptide no TMHs; hypothetical protein 1551510 4608574 azo1419 Azoarcus sp. BH72 hypothetical protein YP_932923.1 1550659 D 62928 CDS YP_932924.1 119897711 4606848 complement(1551523..1552323) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV3790 of Chromobacterium violaceum of 42% (trembl|Q7NRJ1). No domains predicted. no signal peptide 5 TMHs; Conserved hypothetical protein; hypothetical protein 1552323 4606848 azo1420 Azoarcus sp. BH72 hypothetical protein YP_932924.1 1551523 R 62928 CDS YP_932925.1 119897712 4606849 complement(1552425..1553234) 1 NC_008702.1 TREMBLNEW:CAE27009: Probable carboxylesterase, 50% identity, 60% similarity. Dihydrolipoamide acetyltransferase component of acetoin cleaving system (EC 2.3.1.12) (Acetoin dehydrogenase E2 component). InterPro: Alpha/beta hydrolase fold: IPR000073; A/b_hydrolase. IPR003089; AB_hydrolase. IPR000379; Ser_estrs. Pfam PF00561; Abhydrolase_1 InterPro: Alpha/beta hydrolase fold kdgT: 2-keto-3-deoxygluconate permease; Family membership; carboxylesterase 1553234 4606849 azo1421 Azoarcus sp. BH72 carboxylesterase YP_932925.1 1552425 R 62928 CDS YP_932926.1 119897713 4606850 complement(1553238..1554542) 1 NC_008702.1 catalyzes the formation of L-methionine and acetate from O-acetyl-L-homoserine and methanethiol; O-acetylhomoserine aminocarboxypropyltransferase 1554542 4606850 azo1422 Azoarcus sp. BH72 O-acetylhomoserine aminocarboxypropyltransferase YP_932926.1 1553238 R 62928 CDS YP_932927.1 119897714 4606851 complement(1554602..1554817) 1 NC_008702.1 Conserved hypothetical protein. Homology to bsr7110 of B.japonicum of 48% (tremble:Q89EH5). Pfam:Protein of unknown function (DUF465) Family members are found in small bacterial proteins, and also in the heavy chains of eukaryotic myosin and kinesin, C terminal of the motor domain (Myosin Myosin_head, Kinesin Kinesin). Members of this family may form coiled coil structures. No signal peptide present. No TMH; hypothetical protein 1554817 4606851 azo1423 Azoarcus sp. BH72 hypothetical protein YP_932927.1 1554602 R 62928 CDS YP_932928.1 119897715 4606852 1554874..1555542 1 NC_008702.1 necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreB 1555542 greB 4606852 greB Azoarcus sp. BH72 transcription elongation factor GreB YP_932928.1 1554874 D 62928 CDS YP_932929.1 119897716 4608936 1555626..1557557 1 NC_008702.1 molecular chaperone; heat shock protein 90 1557557 htpG 4608936 htpG Azoarcus sp. BH72 heat shock protein 90 YP_932929.1 1555626 D 62928 CDS YP_932930.1 119897717 4609039 complement(1557627..1558949) 1 NC_008702.1 Chloride channel protein, yadQ.In E.coli the product of yadQ gene is named EriC.This family of ion channels contains 10 or 12 transmembrane helices. Each protein forms a single pore. It has been shown that some members of this family form homodimers. These proteins contain two CBS domains. Voltage-gated ClC-type chloride channel clcA. Probably acts as an electrical shunt for an outwardly- directed proton pump that is linked to amino acid decarboxylation as part of the extreme acid resistance (XAR) response (By similarity).; High confidence in function and specificity; putative chloride channel protein 1558949 yadQ 4609039 yadQ Azoarcus sp. BH72 putative chloride channel protein YP_932930.1 1557627 R 62928 CDS YP_932931.1 119897718 4610136 1559286..1559798 1 NC_008702.1 Putative MarR-family transcriptional regulator,; Family membership; MarR family transcriptional regulator 1559798 mexR 4610136 mexR Azoarcus sp. BH72 MarR family transcriptional regulator YP_932931.1 1559286 D 62928 CDS YP_932932.1 119897719 4609214 1560019..1560789 1 NC_008702.1 Conserved hypothetical flavoprotein. Homology to rsc2212 of P. solanacearum of 59% (trembl|Q8XXA4). Pfam: Methallo-beta-lactamase no signal peptide no TMHs; Conserved hypothetical protein; flavoprotein 1560789 4609214 azo1428 Azoarcus sp. BH72 flavoprotein YP_932932.1 1560019 D 62928 CDS YP_932933.1 119897720 4606853 complement(1560826..1562004) 1 NC_008702.1 Conserved hypothetical protein, 63% Identitcal (77% similarity) to TrEMBL;Q8Y0P7,65% identity to TrEMBL;Q7WCK6. Most good homologous hits do not have the same VWA domain represented by this. Has PF05762(IPR008912):VWA domain containing CoxE-like protein;This family is annotated by SMART as containing a VWA type domain. The exact function of this family is unknown. It is found as part of a CO oxidising (Cox) system operon is several bacteria. No Signal peptide or TMH reported present.; Function unclear; hypothetical protein 1562004 4606853 azo1429 Azoarcus sp. BH72 hypothetical protein YP_932933.1 1560826 R 62928 CDS YP_932934.1 119897721 4606854 complement(1562015..1563343) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to TdenA01001626 of Thiobacillus denitrificans of 32% (gi|52006684|ref|ZP_00334063.1|(NBCI ENTREZ)). No domains predicted. Signal P reporting signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 1563343 4606854 azo1430 Azoarcus sp. BH72 hypothetical protein YP_932934.1 1562015 R 62928 CDS YP_932935.1 119897722 4606855 complement(1563353..1564201) 1 NC_008702.1 TREMBL: Q8Y0P5 : 81% identity, 90% similarity. Probable Cell division protein ftsH homolog (EC 3.4.24.-). InterPro:IPR003593; AAA_ATPase. IPR003959; AAA_ATPase_centr. Pfam: PF00004; AAA; ATPase family associated with various SMART: SM00382; AAA; Absence of transmembrane helices (TMHMM predicted) NON secretory protein ruvB: Holliday junction DNA helicase Ru; Specificity unclear; hypothetical protein 1564201 4606855 azo1431 Azoarcus sp. BH72 hypothetical protein YP_932935.1 1563353 R 62928 CDS YP_932936.1 119897723 4606856 1564276..1564599 1 NC_008702.1 Conserved hypothetical cytochrome c-type protein. Homology to rsc0999 of R. solanacearum of 62% (trembl|Q8Y0P4). Pfam: Cytochrome c signal peptide no TMHs; Conserved hypothetical protein; cytochrome c-type protein 1564599 4606856 azo1432 Azoarcus sp. BH72 cytochrome c-type protein YP_932936.1 1564276 D 62928 CDS YP_932937.1 119897724 4606857 1564596..1564937 1 NC_008702.1 Conserved hypothetical cytochrome c-type protein. Homology to ebA4874 of Azoarcus_EbN1 of 60% (gnl|keqq|eba:ebA4874(KEGG)). Interpro: cytochrome c,classI (IPRoo3088); cytochrome c, class IC (IPR008168). Pfam: cytochrome C. singal peptide. no TMHs; Conserved hypothetical protein; cytochrome c-type protein 1564937 4606857 azo1433 Azoarcus sp. BH72 cytochrome c-type protein YP_932937.1 1564596 D 62928 CDS YP_932938.1 119897725 4606858 complement(1565036..1565467) 1 NC_008702.1 Hypothetical protein similar by 78% to RSc1001 [RS04291] [Ralstonia solanacearum(Pseudomonas solanacearum)].TrEMBL Q8Y0P2. No Signal Peptide Reported No TMH Reported.; hypothetical protein 1565467 4606858 azo1434 Azoarcus sp. BH72 hypothetical protein YP_932938.1 1565036 R 62928 CDS YP_932939.1 119897726 4606859 complement(1565496..1566137) 1 NC_008702.1 Endonuclease III . Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines thymines and guanines. Acts on a damaged strand 5 from the damaged site (By similarity). TIGRFAM: comE: comEA protein; High confidence in function and specificity; DNA-(apurinic or apyrimidinic site) lyase 1566137 nth 4606859 nth Azoarcus sp. BH72 DNA-(apurinic or apyrimidinic site) lyase YP_932939.1 1565496 R 62928 CDS YP_932940.1 119897727 4609412 complement(1566139..1566852) 1 NC_008702.1 in Excherichia coli RsxABCDEG reduces SoxR which turns off induction of SoxS transcription factor in the absence of oxidizing agents; similar to the rnfABCDGE operon in Rhodobacter capsulatus involved in transferring electrons to nitrogenase; electron transport complex subunit RsxE 1566852 rnfE2 4609412 rnfE2 Azoarcus sp. BH72 electron transport complex subunit RsxE YP_932940.1 1566139 R 62928 CDS YP_932941.1 119897728 4609749 complement(1566849..1567523) 1 NC_008702.1 Putative electron transport complex protein rnfG. Homolog to rnfG of R. capsulata of 36% (sprot|RNFG_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. signal peptide no TMHs; High confidence in function and specificity; putative electron transport complex protein RnfG 1567523 rnfG2 4609749 rnfG2 Azoarcus sp. BH72 putative electron transport complex protein RnfG YP_932941.1 1566849 R 62928 CDS YP_932942.1 119897729 4609751 complement(1567520..1568527) 1 NC_008702.1 Putative electron transport complex protein rnfD. Homology to rnfD of R. capsulatus of 33% (sprot|RNFD_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase (By similarity). InterPro: NQR2 and RnfD/E related proteins (IPR004338) Pfam: NQR2, RnfD, RnfE family signal peptide probable 6 TMHs; Family membership; putative electron transport complex protein RnfD 1568527 rnfD2 4609751 rnfD2 Azoarcus sp. BH72 putative electron transport complex protein RnfD YP_932942.1 1567520 R 62928 CDS YP_932943.1 119897730 4609747 complement(1568524..1570182) 1 NC_008702.1 part of membrane-bound complex thought to be involved in electron transport to nitrogen; electron transport complex protein RnfC 1570182 rnfC2 4609747 rnfC2 Azoarcus sp. BH72 electron transport complex protein RnfC YP_932943.1 1568524 R 62928 CDS YP_932944.1 119897731 4609745 complement(1570179..1570730) 1 NC_008702.1 part of membrane-bound complex thought to be involved in electron transport to nitrogen; electron transport complex protein RnfB 1570730 rnfB2 4609745 rnfB2 Azoarcus sp. BH72 electron transport complex protein RnfB YP_932944.1 1570179 R 62928 CDS YP_932945.1 119897732 4609743 complement(1570739..1571323) 1 NC_008702.1 Probable electron transport complex protein RnfA. Homology to rnfA of R. capsultatus of 57% (RNFA_RHOCA). Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Required for stable existence of rnfB and rnfC. InterPro: RnfA-Nqr electron transport subunit (IPR003667) Pfam: Rnf-Nqr subunit, membrane protein no signal peptide 6 TMHs; High confidence in function and specificity; electron transport complex promplex protein RnfA 1571323 rnfA2 4609743 rnfA2 Azoarcus sp. BH72 electron transport complex promplex protein RnfA YP_932945.1 1570739 R 62928 CDS YP_932946.1 119897733 4609741 complement(1571407..1572036) 1 NC_008702.1 HNH family of nucleases is found in bacteria,viruses, eukaryotes and includes yeast intron 1 protein,MutS, and bacterial colicins and pyocins. InterPro (IPR003615): HNH nuclease InterPro (IPR002711): HNH endonuclease. Pfam (PF01844): HNH endonuclease.; Family membership; putative nuclease 1572036 4609741 azo1442 Azoarcus sp. BH72 putative nuclease YP_932946.1 1571407 R 62928 CDS YP_932947.1 119897734 4606860 complement(1572157..1572723) 1 NC_008702.1 Conserved hypothetical NUDIX hydrolase. Homology to ne1158 of N. europaea of 58% (trembl|Q82VD6) The generic name 'NUDIX hydrolases' (NUcleoside DIphosphate linked to some other moiety X) has been coined for this domain family. The family can be divided into a number of subgroups, of which MutT anti-mutagenic activity represents only one type; most of the rest hydrolyse diverse nucleoside diphosphate derivatives (including ADP-ribose, GDP- mannose, TDP-glucose, NADH, UDP-sugars, dNTP and NTP). InterPro: NUDIX hydrolase (IPR000086) Pfam: MutT-like domain no signal peptide no TMHs; Function unclear; NUDIX hydrolase 1572723 4606860 azo1443 Azoarcus sp. BH72 NUDIX hydrolase YP_932947.1 1572157 R 62928 CDS YP_932948.1 119897735 4606861 1572893..1574863 1 NC_008702.1 Probable response regulator,; Family membership; response regulator 1574863 mmoQ 4606861 mmoQ Azoarcus sp. BH72 response regulator YP_932948.1 1572893 D 62928 CDS YP_932949.1 119897736 4609229 1574860..1576590 1 NC_008702.1 EAL/GGDEF-domain containing signaling protein,; Conserved hypothetical protein; EAL/GGDEF domain-containing protein 1576590 4609229 azo1445 Azoarcus sp. BH72 EAL/GGDEF domain-containing protein YP_932949.1 1574860 D 62928 CDS YP_932950.1 119897737 4606862 1576751..1577074 1 NC_008702.1 Flagellar transcriptional activator,flhD. This family consists of several bacterial flagellar transcriptional activator proteins. FlhD combines with FlhC to form a regulatory complex in Escherichia coli. This complex has been shown to be a global regulator involved in many cellular processes as well as a flagellar transcriptional activator TrEMBL: Q82SD3 InterPro:IPR007911 Pfam:PF05247 FUNCTION: Transcriptional activator. Involved in the regulation of a number of genetic systems (By similarity). SUBUNIT: Homodimer; disulfide-linked. Forms a heterotetrameric complex with two subunits of flhC (By similarity). SUBCELLULAR LOCATION: Cytoplasmic (By similarity). Belongs to the flhD family. No Signal Peptide. No TMH Present.; Family membership; flagellar transcriptional activator FlhD 1577074 flhD 4606862 flhD Azoarcus sp. BH72 flagellar transcriptional activator FlhD YP_932950.1 1576751 D 62928 CDS YP_932951.1 119897738 4608792 1577079..1577633 1 NC_008702.1 with FlhD is involved in the activation of class 2 flagellar genes and as well as a number of other genetic systems; transcriptional activator FlhC 1577633 flhC 4608792 flhC Azoarcus sp. BH72 transcriptional activator FlhC YP_932951.1 1577079 D 62928 CDS YP_932952.1 119897739 4608791 1577773..1578627 1 NC_008702.1 Flagellar motor protein MotA (Chemotaxis motA protein). Required for rotation of the flagellar motor. Probable transmembrane proton channel (By similarity). signal peptide probable 4 TMHs; High confidence in function and specificity; flagellar motor protein MotA 1578627 motA2 4608791 motA2 Azoarcus sp. BH72 flagellar motor protein MotA YP_932952.1 1577773 D 62928 CDS YP_932953.1 119897740 4609261 1578688..1579761 1 NC_008702.1 Flagellar motor protein MotB (Chemotaxis motB protein. Required for the rotation of the flagellar motor. Might be a linker that fastens the torque-generating machinery to the cell wall. Pfam: OmpA family no signal peptide probable 1 TMHs; High confidence in function and specificity; flagellar motor protein MotB 1579761 motB2 4609261 motB2 Azoarcus sp. BH72 flagellar motor protein MotB YP_932953.1 1578688 D 62928 CDS YP_932954.1 119897741 4609263 1579813..1580229 1 NC_008702.1 Putative chemotaxis response regulator,; Family membership; putative chemotaxis response regulator 1580229 cheY2 4609263 cheY2 Azoarcus sp. BH72 putative chemotaxis response regulator YP_932954.1 1579813 D 62928 CDS YP_932955.1 119897742 4608477 1580242..1582332 1 NC_008702.1 Chemotaxis protein cheA; INVOLVED IN THE TRANSMISSION OF SENSORY SIGNALS FROM THE CHEMORECEPTORS TO THE FLAGELLAR MOTORS. CHEA IS AUTOPHOSPHORYLATED; IT CAN TRANSFER ITS PHOSPHATE GROUP TO EITHER CHEB OR CHEY. EMBL AE005411; AAG56878.1; -. AP002559; BAB36021.1; -. PIR B85802; B85802. F90953; F90953. InterPro IPR003594; ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR002545; CheW. IPR000629; DEAD_box. IPR004105; H-kinase_dim. IPR005467; His_kinase. IPR009082; His_kin_homodim. IPR008207; Hpt. IPR008208; Hpt_N. Pfam PF01584; CheW; 1. PF02895; H-kinase_dim; 1. PF02518; HATPase_c; 1. PF01627; Hpt; 1. PRINTS PR00344; BCTRLSENSOR.; High confidence in function and specificity; chemotaxis protein cheA 1582332 cheA2 4608477 cheA2 Azoarcus sp. BH72 chemotaxis protein cheA YP_932955.1 1580242 D 62928 CDS YP_932956.1 119897743 4608465 1582399..1582908 1 NC_008702.1 Positive regulator of CheA protein activity,; High confidence in function and specificity; positive regulator of CheA protein activity 1582908 cheW2 4608465 cheW2 Azoarcus sp. BH72 positive regulator of CheA protein activity YP_932956.1 1582399 D 62928 CDS YP_932957.1 119897744 4608475 1582905..1584779 1 NC_008702.1 The Escherichia coli chemoreceptor Tsr mediates tactic responses to serine, repellents, and changes in temperature. Similar to sprot|MCP1_ECOLI (31%)and to trembl|Q7NSI6 (36%). Bacterial chemotactic-signal transducers are proteins that respond to changes in the concentration of attractants and repellents in the environment, and transduce a signal from the outside to the inside of the cell. These proteins undergo two covalent modifications: deamidation and reversible methylation. Attractants increase the level of methylation while repellents decrease it. The methyl groups are added by the methyl-transferase cheR and are removed by the methylesterase cheB. Pfam (PF00015): MCPsignal TMHMM reporting two TMH.; Specificity unclear; putative serine chemoreceptor protein 1584779 tsr 4608475 tsr Azoarcus sp. BH72 putative serine chemoreceptor protein YP_932957.1 1582905 D 62928 CDS YP_932958.1 119897745 4610046 1584776..1587298 1 NC_008702.1 Salmonella typhimurium shows an attractant response to citrate and a repellent response to phenol, and a chemoreceptor mediating these responses has been identified and named Tcp (taxis to citrate and away from phenol). Similar to sprot|MCPC_SALTY (32%)and to trembl|Q82TM2. InterPro (IPR004089): Bacterial chemotaxis sensory transducer InterPro (IPR003660): HAMP InterPro (IPR004090): Methyl-accepting chemotaxis protein InterPro (IPR001610): PAC InterPro (IPR000014): PAS_domain Pfam (PF00672): HAMP Pfam (PF00015): MCPsignal Pfam (PF00785): PAC Pfam (PF00989):PAS TIGRFAMs (TIGR00229): sensory_box TMHMM reporting two TMH.; Specificity unclear; putative citrate chemoreceptor protein 1587298 tcp 4610046 tcp Azoarcus sp. BH72 putative citrate chemoreceptor protein YP_932958.1 1584776 D 62928 CDS YP_932959.1 119897746 4609924 1587301..1588254 1 NC_008702.1 Methylase of chemotaxis methyl-accepting proteins,; High confidence in function and specificity; chemotaxis protein methyltransferase 1588254 cheR2 4609924 cheR2 Azoarcus sp. BH72 chemotaxis protein methyltransferase YP_932959.1 1587301 D 62928 CDS YP_932960.1 119897747 4608471 1588263..1589384 1 NC_008702.1 Chemotaxis response regulator protein-glutamate methylesterase, catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR.; High confidence in function and specificity; protein glutamate-methylesterase 1589384 cheB2 4608471 cheB2 Azoarcus sp. BH72 protein glutamate-methylesterase YP_932960.1 1588263 D 62928 CDS YP_932961.1 119897748 4608468 1589532..1591208 1 NC_008702.1 Methyl-accepting chemotaxis protein,; Specificity unclear; methyl accepting chemotaxis protein 1591208 4608468 azo1457 Azoarcus sp. BH72 methyl accepting chemotaxis protein YP_932961.1 1589532 D 62928 CDS YP_932962.1 119897749 4606863 1591411..1592376 1 NC_008702.1 Probable chemotaxis protein, CheW. IPR001789; Response_reg. Pfam: PF01584; CheW. PF00072; Response_reg. SMART: SM00260; CheW. SM00448; REC. Chemotaxis protein cheV. CHEMOTAXIS INVOLVES BOTH A PHOSPHORYLATION-DEPENDENT EXCITATION AND A METHYLATION-DEPENDENT ADAPTATION. CHEV AND CHEW FUNCTION TOGETHER TO COUPLE CHEA ACTIVATION TO METHYL-ACCEPTING CHEMOTAXIS PROTEIN RECEPTOR STATUS AND POSSIBLE CHEA-DEPENDENT PHOSPHORYLATION OF CHEV CONTRIBUTES TO ADAPTATION.; High confidence in function and specificity; chemotaxis protein CheV-like 1592376 cheV1 4606863 cheV1 Azoarcus sp. BH72 chemotaxis protein CheV-like YP_932962.1 1591411 D 62928 CDS YP_932963.1 119897750 4608472 1592386..1593342 1 NC_008702.1 Probable chemotaxis protein, CheW. IPR001789; Response_reg. Pfam: PF01584; CheW. PF00072; Response_reg. SMART: SM00260; CheW. SM00448; REC. Chemotaxis protein cheV. CHEMOTAXIS INVOLVES BOTH A PHOSPHORYLATION-DEPENDENT EXCITATION AND A METHYLATION-DEPENDENT ADAPTATION. CHEV AND CHEW FUNCTION TOGETHER TO COUPLE CHEA ACTIVATION TO METHYL-ACCEPTING CHEMOTAXIS PROTEIN RECEPTOR STATUS AND POSSIBLE CHEA-DEPENDENT PHOSPHORYLATION OF CHEV CONTRIBUTES TO ADAPTATION.; High confidence in function and specificity; chemotaxis protein CheV-like 1593342 cheV2 4608472 cheV2 Azoarcus sp. BH72 chemotaxis protein CheV-like YP_932963.1 1592386 D 62928 CDS YP_932964.1 119897751 4608473 1593373..1593774 1 NC_008702.1 Chemotaxis response regulator, Response_reg. Pfam: PF00072; Response_reg. SMART: SM00448; REC. Chemotaxis protein cheY. Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form cheY exhibits enhanced binding to a switch component fliM at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation (By similarity).; High confidence in function and specificity; chemotaxis response regulator 1593774 cheY3 4608473 cheY3 Azoarcus sp. BH72 chemotaxis response regulator YP_932964.1 1593373 D 62928 CDS YP_932965.1 119897752 4608478 1593809..1594702 1 NC_008702.1 cytosolic phosphatase which functions in the chemotaxis signal transduction complex by controlling the level of phosphorylated CheY through dephosphorylation; chemotaxis regulator CheZ 1594702 cheZ 4608478 cheZ Azoarcus sp. BH72 chemotaxis regulator CheZ YP_932965.1 1593809 D 62928 CDS YP_932966.1 119897753 4608479 1594779..1596674 1 NC_008702.1 Chemotaxis histidine kinase,ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR002545; CheW. IPR004105; H-kinase_dim. IPR005467; His_kinase. IPR008207; Hpt. IPR008208; Hpt_N. Pfam: PF01584; CheW. PF02895; H-kinase_dim. PF02518; HATPase_c. PF01627; Hpt. SMART: SM00260; CheW. SM00387; HATPase_c. SM00073; HPT. Chemotaxis protein cheA (EC 2.7.3.-). INVOLVED IN THE TRANSMISSION OF SENSORY SIGNALS FROM THE CHEMORECEPTORS TO THE FLAGELLAR MOTORS. CHEA IS AUTOPHOSPHORYLATED; IT CAN TRANSFER ITS PHOSPHATE GROUP TO EITHER CHEB OR CHEY.; High confidence in function and specificity; chemotaxis protein cheA 1596674 cheA3 4608479 cheA3 Azoarcus sp. BH72 chemotaxis protein cheA YP_932966.1 1594779 D 62928 CDS YP_932967.1 119897754 4608466 1596794..1597105 1 NC_008702.1 Hypothetical protein, 31% identity (47% similarity) to TrEMBL:Q8KLF6 Coils2 program reports the presence of coiled-coil.; hypothetical protein 1597105 4608466 azo1463 Azoarcus sp. BH72 hypothetical protein YP_932967.1 1596794 D 62928 CDS YP_932968.1 119897755 4606864 complement(1597121..1598236) 1 NC_008702.1 catalyzes the reversible formation of D-erythrose 4-phosphate and D-fructose 6-phosphate from sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate; transaldolase 1598236 tal 4606864 tal Azoarcus sp. BH72 transaldolase YP_932968.1 1597121 R 62928 CDS YP_932969.1 119897756 4609913 complement(1598208..1598990) 1 NC_008702.1 tRNA pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil-65 in transfer RNAs; High confidence in function and specificity; ORF3; hypothetical protein 1598990 4609913 azo1465 Azoarcus sp. BH72 hypothetical protein YP_932969.1 1598208 R 62928 CDS YP_932970.1 119897757 4606865 complement(1599073..1599657) 1 NC_008702.1 SodB: Superoxide dismutase [Fe] (EC 1.15.1.1).Catalyse the conversion of superoxide radicals to molecular oxygen. Their function is to destroy the radicals that are normally produced within cells and are toxic to biological systems. 76% SODismutase. Pfam:PF02777; Sod_Fe_C; 1. PF00081; Sod_Fe_N; 1.; High confidence in function and specificity; putative superoxide dismutase 1599657 4606865 azo1466 Azoarcus sp. BH72 putative superoxide dismutase YP_932970.1 1599073 R 62928 CDS YP_932971.1 119897758 4606866 complement(1599814..1601193) 1 NC_008702.1 Probable exodeoxyribonuclease VII large subunit . Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides which are then degraded further into small acid-soluble oligonucleotides (By similarity). TIGRFAM: xseA: exodeoxyribonuclease VII large.; High confidence in function and specificity; exodeoxyribonuclease VII 1601193 xseA 4606866 xseA Azoarcus sp. BH72 exodeoxyribonuclease VII YP_932971.1 1599814 R 62928 CDS YP_932972.1 119897759 4610129 1601484..1602092 1 NC_008702.1 conserved hypothetical biopolymer transport protein ExbB. Homology to exbB of B. pertussis. ExbB is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. Pfam: MotA/TolQ/ExbB proton channel family no signal peptide probable 3 TMHS; Family membership; biopolymer transport protein ExbB 1602092 exbB3 4610129 exbB3 Azoarcus sp. BH72 biopolymer transport protein ExbB YP_932972.1 1601484 D 62928 CDS YP_932973.1 119897760 4608698 1602104..1602526 1 NC_008702.1 Putative biopolymer transport exbD2 protein. Homology to exbD2 of V. choloera of 31% ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; Family membership; putative biopolymer transport protein ExbD 1602526 exbD4 4608698 exbD4 Azoarcus sp. BH72 putative biopolymer transport protein ExbD YP_932973.1 1602104 D 62928 CDS YP_932974.1 119897761 4608703 1602530..1603555 1 NC_008702.1 transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate; tetraacyldisaccharide 4'-kinase 1603555 lpxK 4608703 lpxK Azoarcus sp. BH72 tetraacyldisaccharide 4'-kinase YP_932974.1 1602530 D 62928 CDS YP_932975.1 119897762 4609179 1603536..1603724 1 NC_008702.1 Putative inner membrane protein [ycaR], 66% identity (85% similarity) to SwissProt;P75844(E.coli) and 65% identity to TrEMBL;Q7CQT7. Has PF03966:Protein of unknown function (DUF343);This family of short proteins have no known function. The bacterial members are about 60-70 amino acids in length and the eukaryotic examples are about 120 amino acids in length. The C terminus contains the strongest conservation. Signal Peptide or TMH not present.; Function unclear; hypothetical protein 1603724 ycaR 4609179 ycaR Azoarcus sp. BH72 hypothetical protein YP_932975.1 1603536 D 62928 CDS YP_932976.1 119897763 4610152 1603733..1604506 1 NC_008702.1 CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS; 3-deoxy-manno-octulosonate cytidylyltransferase 1604506 kdsB 4610152 kdsB Azoarcus sp. BH72 3-deoxy-manno-octulosonate cytidylyltransferase YP_932976.1 1603733 D 62928 CDS YP_932977.1 119897764 4609104 1604626..1605279 1 NC_008702.1 essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP; adenylate kinase 1605279 adk 4609104 adk Azoarcus sp. BH72 adenylate kinase YP_932977.1 1604626 D 62928 CDS YP_932978.1 119897765 4608282 1605395..1606660 1 NC_008702.1 catalyzes the formation of D-fructose 1,6-bisphosphate from D-fructose 6-phosphate in glycolysis; 6-phosphofructokinase 1606660 pfkA 4608282 pfkA Azoarcus sp. BH72 6-phosphofructokinase YP_932978.1 1605395 D 62928 CDS YP_932979.1 119897766 4609528 1606678..1608399 1 NC_008702.1 Gamma-glutamyltranspeptidase catalyzes the transfer of the gamma-glutamyl moiety of glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione and drug and xenobiotic detoxification. Similar to trembl|Q88H30 (43%)and to trembl|Q8UJH0 (36%). TIGRfam (TIGR00066): Gamma-glutamyltranspeptidase Pfam (PF01019): Gamma-glutamyltranspeptidase SignalP reporting Signal peptide.; Specificity unclear; Ggt protein 1608399 ggt 4609528 ggt Azoarcus sp. BH72 Ggt protein YP_932979.1 1606678 D 62928 CDS YP_932980.1 119897767 4608875 complement(1608393..1610963) 1 NC_008702.1 Conserved hypothetical membrane protein,; Conserved hypothetical protein; hypothetical protein 1610963 4608875 azo1476 Azoarcus sp. BH72 hypothetical protein YP_932980.1 1608393 R 62928 CDS YP_932981.1 119897768 4606867 complement(1611123..1611704) 1 NC_008702.1 Putative NAD(P)H oxidoreductase HI1544 (EC 1.6.5.2 ). trembl:Q88J60: 64% identity; 80% similarity This family consists of bacterial and eukaryotic NAD(P)H dehydrogenase (quinone) EC: 1.6.5.2 that catalyses the NAD(P)H-dependent two-electron reductions of quinones and protect cells against damage by free radicals and reactive oxygen species [1]. This enzyme uses a FAD co-factor InterPro:IPR003680; NADHdh_2. Pfam:PF02525; Flavodoxin_2 SignalP predicted signal peptide present No transmembrane helices (TMHMM predicted); High confidence in function and specificity; NAD(P)H quinone oxidoreductase 1611704 drgA 4606867 drgA Azoarcus sp. BH72 NAD(P)H quinone oxidoreductase YP_932981.1 1611123 R 62928 CDS YP_932982.1 119897769 4608651 complement(1611764..1612186) 1 NC_008702.1 Probable MerR-family transcriptional regulator,; Family membership; MerR family transcriptional regulator 1612186 4608651 azo1478 Azoarcus sp. BH72 MerR family transcriptional regulator YP_932982.1 1611764 R 62928 CDS YP_932983.1 119897770 4606868 1612279..1613682 1 NC_008702.1 Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation; glutamyl-tRNA synthetase 1613682 gltX 4606868 gltX Azoarcus sp. BH72 glutamyl-tRNA synthetase YP_932983.1 1612279 D 62928 CDS YP_932984.1 119897771 4608918 complement(1613749..1614060) 1 NC_008702.1 Probable Nitrogen regulatory protein P-II 1. Could be involved in the regulation of nitrogen fixation.Belongs to the P(II) protein family.55% similarity with Methanothermobacter thermautotrophicus,TrEMBL:O26758 Pfam:PF00543, InterPro:IPR002332,IPR002187 No Signal peptide present. No TMH reported.; nitrogen regulatory protein P-II 1614060 4608918 azo1480 Azoarcus sp. BH72 nitrogen regulatory protein P-II YP_932984.1 1613749 R 62928 CDS YP_932985.1 119897772 4606869 complement(1614063..1615001) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ttc139 of T. termophilus of 49% (trembl|Q72LC0). no domains no singal peptide 8 TMHs; Conserved hypothetical protein; hypothetical protein 1615001 4606869 azo1481 Azoarcus sp. BH72 hypothetical protein YP_932985.1 1614063 R 62928 CDS YP_932986.1 119897773 4606870 complement(1615045..1616952) 1 NC_008702.1 catalyzes the formation of 5-methylaminomethyl-2-thiouridine in position 34 of the anticodon of tRNA molecules; 5-methylaminomethyl-2-thiouridine methyltransferase 1616952 mnmC 4606870 mnmC Azoarcus sp. BH72 5-methylaminomethyl-2-thiouridine methyltransferase YP_932986.1 1615045 R 62928 CDS YP_932987.1 119897774 4610171 complement(1617116..1619113) 1 NC_008702.1 Putative 5'-nucleotidase Has Signal Peptide. Has PF02872, 5'-nucleotidase, C-terminal domain; IPR008334, 5'-Nucleotdase_C; 5'-nucleotidases are enzymes that catalyze the hydrolysis ofphosphate esterified at carbon 5' of the ribose and deoxyribose portions of nucleotide molecules. 5'-nucleotidase is a ubiquitous enzyme found in a wide variety of species and which occurs in different cellular locations. The extracellular 5'-nucleotidase from mammals and electric ray isozyme is a homodimeric disulphide-bonded glycoprotein attached to the membrane by a GPI-anchor, and requires zinc for its activity. Has PF00149,Calcineurin-like phosphoesterase; IPR004843, M-pesterase; This family includes a diverse range of phosphoesterases,including protein phosphoserine phosphatases,nucleotidases, sphingomyelin phosphodiesterases and 2'-3' cAMP phosphodiesterases as well as nucleases such as bacterial SbcD P13457 or yeast MRE11 P32829. The most conserved regions in this superfamily centre around the metal chelating residues.; High confidence in function and specificity; 5'-nucleotidase 1619113 4610171 azo1483 Azoarcus sp. BH72 5'-nucleotidase YP_932987.1 1617116 R 62928 CDS YP_932988.1 119897775 4606871 complement(1619180..1622011) 1 NC_008702.1 Putative extracellular nuclease. Homology to nucH of A. hydrophila of 33% (trembl|Q44070) Tigrfam: xth: exodeoxyribonuclease III (xth) Pfam: Endonuclease/ Exonuclease/ Phoshatase family singal peptide no TMHs; Family membership; putative extracellular nuclease 1622011 nucH 4606871 nucH Azoarcus sp. BH72 putative extracellular nuclease YP_932988.1 1619180 R 62928 CDS YP_932989.1 119897776 4609417 1622218..1622907 1 NC_008702.1 Conserved hypothetical membrane protein. Homology ebA4071 of Azoarcus sp. EbN1 of 61% (gnl|keqq|eba:ebA4071(KEGG)). Has PF06930, Protein of unknown function (DUF1282); IPR009698; This family consists of several hypothetical proteins of around 200 residues in length. The function of this family is unknown although a number of family members are thought to be putative membrane proteins. no signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 1622907 4609417 azo1485 Azoarcus sp. BH72 hypothetical protein YP_932989.1 1622218 D 62928 CDS YP_932990.1 119897777 4606872 complement(1622923..1624122) 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to TdenA01000705 of Thiobacillus denitrificans of 61% (gi|52008401|ref|ZP_00335778.1|(NBCI ENTREZ)). Pfam: PF01590 GAF domain. PF00563 EAL domain. No signal peptide. No TMHs.; Conserved hypothetical protein; putative signaling protein 1624122 4606872 azo1486 Azoarcus sp. BH72 putative signaling protein YP_932990.1 1622923 R 62928 CDS YP_932991.1 119897778 4606873 complement(1624335..1625837) 1 NC_008702.1 class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1; lysyl-tRNA synthetase 1625837 lysS 4606873 lysS Azoarcus sp. BH72 lysyl-tRNA synthetase YP_932991.1 1624335 R 62928 CDS YP_932992.1 119897779 4609188 1625995..1626372 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003756 of Dechloromonas aromatica of 53% (gi|41722684|ref|ZP_00149673.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 1626372 4609188 azo1488 Azoarcus sp. BH72 hypothetical protein YP_932992.1 1625995 D 62928 CDS YP_932993.1 119897780 4606874 complement(1626384..1626902) 1 NC_008702.1 Hypothetical protein CT2219. SPROT:Q8KAE4: 65% identity; 78% similarity. TREMBL:Q7NS63: 60% identity, 72% similarity putative ACR related to the C-terminal domain of histone macroH2A1 This domain is found in a number of protein associated with DNA and/or RNA unwinding InterPro; IPR002589; A1pp. Pfam; PF01661; A1pp; 1. SMART; SM00506; A1pp non-secretory protein with no signal peptide (SignalP predicted). No transmembrane helices (TMHMM predicted); Function unclear; hypothetical protein 1626902 4606874 azo1489 Azoarcus sp. BH72 hypothetical protein YP_932993.1 1626384 R 62928 CDS YP_932994.1 119897781 4606875 complement(1627018..1630287) 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to ebA6472 Azoarcus sp. EbN1 of 36% (gnl|keqq|eba:ebA6472(KEGG)). InterPro: IPR000160 GGDEF. IPR000014 PAS. IPR000700 PAS-assoc_C. IPR001789 Response_reg. Pfam: PF00990 GGDEF domain. PF00989 PAS domain. PF00785 PAC motif. PF01590 GAF domain. PF00563 EAL domain. PF00072 Response_reg. TIGRFAM:TIGR00229 PAS domain S-box. TIGR00254 putative diguanylate cyclase (GGDEF) domain. No signal peptide. No TMHs; Conserved hypothetical protein; putative signaling protein 1630287 4606875 azo1490 Azoarcus sp. BH72 putative signaling protein YP_932994.1 1627018 R 62928 CDS YP_932995.1 119897782 4606876 complement(1630292..1630687) 1 NC_008702.1 Probable polar differentiation response regulator,; Family membership; polar differentiation response regulator 1630687 divK 4606876 divK Azoarcus sp. BH72 polar differentiation response regulator YP_932995.1 1630292 R 62928 CDS YP_932996.1 119897783 4608623 complement(1630684..1632525) 1 NC_008702.1 Putative histidine protein kinase,; Specificity unclear; putative histidine protein kinase 1632525 divJ 4608623 divJ Azoarcus sp. BH72 putative histidine protein kinase YP_932996.1 1630684 R 62928 CDS YP_932997.1 119897784 4608622 complement(1632830..1634692) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Avar03001212 of Anabaena variabilis of 34% (gi|53765559|ref|ZP_00161954.2|(NBCI ENTREZ)). Pfam: Peptidase family M48. 2 TMHs. no signal peptide; Conserved hypothetical protein; hypothetical protein 1634692 4608622 azo1493 Azoarcus sp. BH72 hypothetical protein YP_932997.1 1632830 R 62928 CDS YP_932998.1 119897785 4606877 complement(1634819..1637611) 1 NC_008702.1 Similarity to hypothetical protein in C. violaceum.; hypothetical protein 1637611 4606877 azo1494 Azoarcus sp. BH72 hypothetical protein YP_932998.1 1634819 R 62928 CDS YP_932999.1 119897786 4606878 complement(1637617..1638315) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV0860 of Chromobacterium violaceum of 50% (trembl: Q7NZR1). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1638315 4606878 azo1495 Azoarcus sp. BH72 hypothetical protein YP_932999.1 1637617 R 62928 CDS YP_933000.1 119897787 4606879 complement(1638326..1639639) 1 NC_008702.1 Conserved Hypothetical protein Signal Peptide present. Has Prosite PS00380; RHODANESE_1; Rhodanese (thiosulfate sulfurtransferase) (EC 2.8.1.1)is an enzyme which catalyzes the transfer of the sulfane atom of thiosulfate to cyanide, to form sulfite and thiocyanate. In vertebrates, rhodanese is a mitochondrial enzyme of about 300 amino-acid residues involved in forming iron-sulfur complexes and cyanide detoxification. A cysteine residue takes part in the catalytic mechanism.; hypothetical protein 1639639 4606879 azo1496 Azoarcus sp. BH72 hypothetical protein YP_933000.1 1638326 R 62928 CDS YP_933001.1 119897788 4606880 complement(1639694..1640815) 1 NC_008702.1 recognizes the termination signals UGA and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF2; in some organisms control of PrfB protein levels is maintained through a +1 ribosomal frameshifting mechanism; this protein is similar to release factor 1; peptide chain release factor 2 1640815 prfB 4606880 prfB Azoarcus sp. BH72 peptide chain release factor 2 YP_933001.1 1639694 R 62928 CDS YP_933002.1 119897789 4609624 1640905..1641321 1 NC_008702.1 Conserved hypothetical membrane protein Homology to an orf of C. violaceum of 41% (trembl|Q7NSF3(SRS)) No domains predicted Signal Peptide present. TMHMM2 reporting 2 TMH's present.; Conserved hypothetical protein; hypothetical protein 1641321 4609624 azo1498 Azoarcus sp. BH72 hypothetical protein YP_933002.1 1640905 D 62928 CDS YP_933003.1 119897790 4606881 complement(1641332..1641760) 1 NC_008702.1 TREMBL:Q8XSIO: 58% identity, 73% similarity Hypothetical HIT-like protein Rv0759c/MT0784/Mb0782c. InterPro:IPR001310; HIT. Pfam: PF01230; HIT; 1. PRINTS: PR00332; HISTRIAD InterPro: HIT (Histidine triad) family hupD: hydrogenase expression/formation pr; Family membership; HIT family protein 1641760 4606881 azo1499 Azoarcus sp. BH72 HIT family protein YP_933003.1 1641332 R 62928 CDS YP_933004.1 119897791 4606882 1641939..1642796 1 NC_008702.1 Hypothetical secreted protein. Weak homology with hits in the database. Signal Peptide present. No domains predicted. No TMHs; hypothetical protein 1642796 4606882 azo1500 Azoarcus sp. BH72 hypothetical protein YP_933004.1 1641939 D 62928 CDS YP_933005.1 119897792 4606883 1642934..1643425 1 NC_008702.1 Hypothetical protein. No homology to the data bank. Has 2 copies of SMART;SM00671, SEL1, Sel1-like repeats.These represent a subfamily of TPR (tetratricopeptide repeat) sequences. No TMHs. No signal peptide.; hypothetical protein 1643425 4606883 azo1501 Azoarcus sp. BH72 hypothetical protein YP_933005.1 1642934 D 62928 CDS YP_933006.1 119897793 4606884 1643422..1643817 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. signal peptide. 3 TMHs; hypothetical protein 1643817 4606884 azo1502 Azoarcus sp. BH72 hypothetical protein YP_933006.1 1643422 D 62928 CDS YP_933007.1 119897794 4606885 complement(1644105..1644740) 1 NC_008702.1 Putative two-component response regulator,; Family membership; putative two-component response regulator 1644740 4606885 azo1503 Azoarcus sp. BH72 putative two-component response regulator YP_933007.1 1644105 R 62928 CDS YP_933008.1 119897795 4606886 complement(1644737..1646212) 1 NC_008702.1 Putative two-component sensor histidine kinase,; Specificity unclear; putative two-component sensor histidine kinase 1646212 moxY 4606886 moxY Azoarcus sp. BH72 putative two-component sensor histidine kinase YP_933008.1 1644737 R 62928 CDS YP_933009.1 119897796 4609266 complement(1646272..1647963) 1 NC_008702.1 Single-stranded-DNA-specific exonuclease recJ (EC 3.1.-.-). SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE. REQUIRED FOR MANY TYPES OF RECOMBINATIONAL EVENTS ALTHOUGH THE STRINGENCY OF THE REQUIREMENT FOR RECJ APPEARS TO VARY WITH THE TYPE OF RECOMBINATIONAL EVENT MONITORED AND THE OTHER RECOMBINATION GENE PRODUCTS WHICH ARE AVAILABLE. InterPro: Single-stranded-DNA-specific exonuclease RecJ. recJ: single-stranded-DNA-specific exo.; High confidence in function and specificity; single-stranded-DNA-specific exonuclease 1647963 recJ 4609266 recJ Azoarcus sp. BH72 single-stranded-DNA-specific exonuclease YP_933009.1 1646272 R 62928 CDS YP_933010.1 119897797 4609707 complement(1647960..1649036) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2476 of C.violaceum of 38% (tremble:Q7NV68) No domains predicted. No signal peptide or TMH present.; hypothetical protein 1649036 4609707 azo1506 Azoarcus sp. BH72 hypothetical protein YP_933010.1 1647960 R 62928 CDS YP_933011.1 119897798 4606887 1649253..1651382 1 NC_008702.1 Conserved hypothetical protein. Homology to cv1738 of C. violaceum (sprot|YH38_CHRVO) InterPro: Uncharacterized protein family UPF0004 (IPR005839) no signal peptide no TMHs TIGR00089: conserved hypothetical prote; hypothetical protein 1651382 4606887 azo1507 Azoarcus sp. BH72 hypothetical protein YP_933011.1 1649253 D 62928 CDS YP_933012.1 119897799 4606888 complement(1651391..1651927) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domaine predicted. No signal peptide. 2 TMHs; hypothetical protein 1651927 4606888 azo1508 Azoarcus sp. BH72 hypothetical protein YP_933012.1 1651391 R 62928 CDS YP_933013.1 119897800 4606889 1652224..1653480 1 NC_008702.1 Putative lipoprotein transporter permease. Homology to lolE of E. coli of 36% (sprot|LOLE_ECOLI). Part of an ATP-dependent transport system responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of lolA (By similarity). Pfam: Predicted permease signal peptide 3 TMHs; High confidence in function and specificity; putative lipoprotein transporter permease 1653480 lolE1 4606889 lolE1 Azoarcus sp. BH72 putative lipoprotein transporter permease YP_933013.1 1652224 D 62928 CDS YP_933014.1 119897801 4609171 1653473..1654180 1 NC_008702.1 Lipoprotein releasing system ATP-binding protein lolD. Part of an ATP-dependent transport system responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of lolA. Similar to TREMBL:Q82VL9; TREMBL:Q8Y0C6; SWISSPROT:P75957 (58% identity). Pfam (PF00005): ABC transporter. TC (3.A.1.125): The Lipoprotein Translocase (LPT) Family.; High confidence in function and specificity; lipoprotein releasing system ATP-binding protein 1654180 lolD 4609171 lolD Azoarcus sp. BH72 lipoprotein releasing system ATP-binding protein YP_933014.1 1653473 D 62928 CDS YP_933015.1 119897802 4609170 complement(1654177..1654722) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ne0829 of N. europaea of 46% (trembl|Q82W66(SRS)) No domains predicted No signal peptide 4 TMHs; Conserved hypothetical protein; hypothetical protein 1654722 4609170 azo1511 Azoarcus sp. BH72 hypothetical protein YP_933015.1 1654177 R 62928 CDS YP_933016.1 119897803 4606890 1654821..1657130 1 NC_008702.1 Competence protein comA. Essential for natural transformation. Could be a transporter involved in DNA uptake. InterPro: DNA internalization-related competence protein ComEC/Rec2 function: transporter activity TREMBL:Q82SD2: 38% identity,50% similarity. InterPro:IPR001279; Blactmase-like. IPR004477; ComEC_N-term. IPR004797; ComEC_Rec2. Pfam PF03772; Competence; 1. PF00753; Lactamase_B; 1. TIGRFAMs:TIGR00360; ComEC_N-term; 1. TIGR00361; ComEC_Rec2; ComEC_Rec2: DNA internalization-relate TMHMM predicted 8 transmembrane helices; High confidence in function and specificity; putative DNA uptake/competence protein 1657130 comA 4606890 comA Azoarcus sp. BH72 putative DNA uptake/competence protein YP_933016.1 1654821 D 62928 CDS YP_933017.1 119897804 4608510 1657199..1658110 1 NC_008702.1 TREMBL:Q8XZ32: 54% identity, 66% similarity Probable Hypothetical protein Rv2715/MT2788/Mb2734. InterPro:IPR000073; A/b_hydrolase. IPR003089; AB_hydrolase. IPR000379; Ser_estrs. Pfam PF00561; Abhydrolase_1; 1 InterPro: Alpha/beta hydrolase fold Mtu_fam_11: Mycobacterium tuberculosis pa; Family membership; hydrolase 1658110 4608510 azo1513 Azoarcus sp. BH72 hydrolase YP_933017.1 1657199 D 62928 CDS YP_933018.1 119897805 4606891 1658292..1659710 1 NC_008702.1 Conserved hypothetical protein. Homology to RS04633 of R.solanacearum of 73% (tremble:Q8Y0C3). Has PF04169(IPR007297);Domain of unknown function (DUF404);This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF403 (IPR007296) and DUF407 (IPR007302). Has PF04174;Domain of unknown function (DUF407);This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF403 (IPR007296) and DUF404 (IPR007297). No signal Peptide or TMH present.; hypothetical protein 1659710 4606891 azo1514 Azoarcus sp. BH72 hypothetical protein YP_933018.1 1658292 D 62928 CDS YP_933019.1 119897806 4606892 1659734..1660678 1 NC_008702.1 Conserved Hypothetical protein, 57% identity to TrEMBL;Q8Y0C2 Has PF04168;(IPR00729)Bacterial domain of unknown function (DUF403):This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF404 (IPR007297) and DUF407 (IPR007302). No Signal peptide or TMH reported Present.; Function unclear; hypothetical protein 1660678 4606892 azo1515 Azoarcus sp. BH72 hypothetical protein YP_933019.1 1659734 D 62928 CDS YP_933020.1 119897807 4606893 complement(1660725..1661654) 1 NC_008702.1 EngC; RsgA; CpgA; circularly permuted GTPase; ribosome small subunit-dependent GTPase A; has the pattern G4-G1-G3 as opposed to other GTPases; interacts strongly with 30S ribosome which stimulates GTPase activity; ribosome-associated GTPase 1661654 yjeQ 4606893 yjeQ Azoarcus sp. BH72 ribosome-associated GTPase YP_933020.1 1660725 R 62928 CDS YP_933021.1 119897808 4610188 complement(1661656..1662909) 1 NC_008702.1 Putative metalloprotease. Homology to ste24 of S. cerevisiae of 32% (sprot|ST24_YEAST). CAAX prenyl protease 1 (EC 3.4.24.84) (Prenyl protein-specific endoprotease 1) (PPSEP 1) (A-factor converting enzyme). Proteolytically removes the C-terminal three residues of farnesylated A-factor mating pheromone. Also acts to cleave the N-terminal extension of the pheromone. probable signal peptide probable 7 TMHs; Family membership; putative metalloprotease 1662909 4610188 azo1517 Azoarcus sp. BH72 putative metalloprotease YP_933021.1 1661656 R 62928 CDS YP_933022.1 119897809 4606894 1663104..1663652 1 NC_008702.1 3'-5' exoribonuclease specific for small oligoribonuclotides; oligoribonuclease 1663652 orn 4606894 orn Azoarcus sp. BH72 oligoribonuclease YP_933022.1 1663104 D 62928 CDS YP_933023.1 119897810 4609457 complement(1663676..1665385) 1 NC_008702.1 functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family; glucose-6-phosphate isomerase 1665385 pgi 4609457 pgi Azoarcus sp. BH72 glucose-6-phosphate isomerase YP_933023.1 1663676 R 62928 CDS YP_933024.1 119897811 4609530 complement(1665304..1666938) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV1748 of C. violaceum of 32% (trembl|Q7NX80(SRS)). No domains predicted. No signal peptide 11 TMHs; Conserved hypothetical protein; hypothetical protein 1666938 4609530 azo1520 Azoarcus sp. BH72 hypothetical protein YP_933024.1 1665304 R 62928 CDS YP_933025.1 119897812 4606895 complement(1667147..1667365) 1 NC_008702.1 RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 1667365 rpmE 4606895 rpmE Azoarcus sp. BH72 50S ribosomal protein L31 YP_933025.1 1667147 R 62928 CDS YP_933026.1 119897813 4609789 1667410..1668510 1 NC_008702.1 putative TIM-barrel protein; Specificity unclear; hypothetical protein 1668510 4609789 azo1522 Azoarcus sp. BH72 hypothetical protein YP_933026.1 1667410 D 62928 CDS YP_933027.1 119897814 4606896 1668539..1669234 1 NC_008702.1 tRNA (guanine-N(7)-)-methyltransferase, 29% identity to SwissProt;Q8EBX8. SwissProt;Q8Y1I7(25% Identity),Q8FE22(25% identity). Has PF02390,Methyltransf_4,Putative methyltransferase;IPR003358; This is a family of putative methyltransferases. The aligned region contains the GXGXG S-AdoMet binding site suggesting a putative methyltransferase activity.; tRNA (guanine-N7-)-methyltransferase 1669234 yggH 4606896 yggH Azoarcus sp. BH72 tRNA (guanine-N7-)-methyltransferase YP_933027.1 1668539 D 62928 CDS YP_933028.1 119897815 4610177 1669231..1669941 1 NC_008702.1 Conserved hypothetical protein. Homology to ct227 of C. tepidum of 32% (trembl|Q8KA93). Pfam: SEC-C motif This motif is predicted to chelate zinc with the CXC and C[HC] pairs that constitute the most conserved feature of the motif. It is predicted to be a potential nucleic acid binding domain. no TMHs; hypothetical protein 1669941 4610177 azo1524 Azoarcus sp. BH72 hypothetical protein YP_933028.1 1669231 D 62928 CDS YP_933029.1 119897816 4606897 1669934..1670281 1 NC_008702.1 Conserved hypothetical protein. Homology to PA3209 of Pseudomonas aeruginosa of 43% (trembl:Q9HZ31). Has PF03692, Uncharacterised protein family (UPF0153); IPR005358;This family of proteins contain 8 conserved cysteines that may form a metal binding site. The function of these proteins is unknown but might be an Fe-S cluster as part of an oxidoreductase complex. No signal peptide. No TMHs; hypothetical protein 1670281 4606897 azo1525 Azoarcus sp. BH72 hypothetical protein YP_933029.1 1669934 D 62928 CDS YP_933030.1 119897817 4606898 1670316..1670681 1 NC_008702.1 TREMBL:Q8XH16: 56% identity, 66% similarity. SPROT:P71300: 53% identity, 67% similarity Hypothetical protein ykgJ; This family of proteins contain 8 conserved cysteines that may form a zinc binding site. The function of these proteins is unknown InterPro:IPR005358; UPF0153. Pfam: PF03692; UPF0153 No signal peptide. No transmembrane helices; High confidence in function and specificity; ferredoxin 1670681 4606898 azo1526 Azoarcus sp. BH72 ferredoxin YP_933030.1 1670316 D 62928 CDS YP_933031.1 119897818 4606899 complement(1670701..1671576) 1 NC_008702.1 Function unclear; putative ATP adenylyltransferase 1671576 4606899 azo1527 Azoarcus sp. BH72 putative ATP adenylyltransferase YP_933031.1 1670701 R 62928 CDS YP_933032.1 119897819 4606900 1671664..1671888 1 NC_008702.1 Conserved hypothetical protein. Homology to XAC2816 of Xanthomonas axonopodis of 52% (trembl:Q8PIS9). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1671888 4606900 azo1528 Azoarcus sp. BH72 hypothetical protein YP_933032.1 1671664 D 62928 CDS YP_933033.1 119897820 4606901 complement(1671902..1673644) 1 NC_008702.1 42% K+channel_pore.IPR003148; TrkA_N. Pfam:PF02254; TrkA_N; 2. TMhelix:3; Function unclear; fused NAD-binding/uncharacterized domain-containing protein 1673644 4606901 azo1529 Azoarcus sp. BH72 fused NAD-binding/uncharacterized domain-containing protein YP_933033.1 1671902 R 62928 CDS YP_933034.1 119897821 4606902 complement(1673655..1674038) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to AQ_302 of Aquifex aeolicus of 38% (trembl|O66649). No domains predicted. No signal peptide 4 TMHs; Conserved hypothetical protein; hypothetical protein 1674038 4606902 azo1530 Azoarcus sp. BH72 hypothetical protein YP_933034.1 1673655 R 62928 CDS YP_933035.1 119897822 4606903 1674393..1675244 1 NC_008702.1 Hypothetical secreted protein. Weak homology with hits in the database. Signal Peptide present. No TMHs. No domains predicted; hypothetical protein 1675244 4606903 azo1531 Azoarcus sp. BH72 hypothetical protein YP_933035.1 1674393 D 62928 CDS YP_933036.1 119897823 4606904 complement(1675220..1675543) 1 NC_008702.1 Hypothetical protein, 65% Identity (77% similarity) to SwissProt; Q7NS67.56% identity to SwissProt;Q8Y2R5. No domains, repeats, motifs or features present.; hypothetical protein 1675543 4606904 azo1532 Azoarcus sp. BH72 hypothetical protein YP_933036.1 1675220 R 62928 CDS YP_933037.1 119897824 4606905 complement(1675840..1678551) 1 NC_008702.1 Catalyzes the conversion of citrate to isocitrate; aconitate hydratase 1678551 acnA 4606905 acnA Azoarcus sp. BH72 aconitate hydratase YP_933037.1 1675840 R 62928 CDS YP_933038.1 119897825 4608263 1678824..1681421 1 NC_008702.1 catalyzes the conversion of citrate to isocitrate and the conversion of 2-methylaconitate to 2-methylisocitrate; bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase 1681421 acnB 4608263 acnB Azoarcus sp. BH72 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase YP_933038.1 1678824 D 62928 CDS YP_933039.1 119897826 4608264 1681615..1684014 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to ebA3785 of Azoarcus sp. EbN1 of 40% (gnl|keqq|eba:ebA3785(KEGG)). Pfam: PF00990 GGDEF domain. PF01590 GAF domain. PF00563 EAL domain. TIGRFAM:TIGR00254 putative diguanylate cyclase (GGDEF) domain. No signal peptide. No TMHs.; Conserved hypothetical protein; putative signaling protein 1684014 4608264 azo1535 Azoarcus sp. BH72 putative signaling protein YP_933039.1 1681615 D 62928 CDS YP_933040.1 119897827 4606906 1684130..1684609 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bd2668 of B. bacteriovorus of 53% (tremblnew|CAE80459). no domains signal peptide no TMHs; Conserved hypothetical protein; hypothetical protein 1684609 4606906 azo1536 Azoarcus sp. BH72 hypothetical protein YP_933040.1 1684130 D 62928 CDS YP_933041.1 119897828 4606907 1684668..1685216 1 NC_008702.1 Conserved hypothetical protein. Homology to Raeut03004638 of Ralstonia eutropha of 37% (gi|45515835|ref|ZP_00167389.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 1685216 4606907 azo1537 Azoarcus sp. BH72 hypothetical protein YP_933041.1 1684668 D 62928 CDS YP_933042.1 119897829 4606908 1685213..1686571 1 NC_008702.1 Putative phosphoenolpyruvate-protein phosphotransferase,; Function unclear; putative phosphoenolpyruvate-protein phosphotransferase 1686571 ptsp 4606908 ptsp Azoarcus sp. BH72 putative phosphoenolpyruvate-protein phosphotransferase YP_933042.1 1685213 D 62928 CDS YP_933043.1 119897830 4609652 1686698..1687660 1 NC_008702.1 Putative acetoin dehydrogenase, alpha subunit. Homology to acoA of C. magnum of 36% (trembl|Q46142) CATALYZES THE 26-DICHLOROPHENOLINDOPHENOL-DEPENDENT CLEAVAGE OF ACETOIN INTO ACETATE AND ACETALDEHYDE IN VITRO. THE ALPHA SUBUNIT IS PROBABLY THE CATALYTIC SUBUNIT OF THE ENZYME. Pfam: Dehydrogenase E1 component no signal peptide no TMHs; Family membership; putative acetion dehydrogenase subunit alpha 1687660 acoA1 4609652 acoA1 Azoarcus sp. BH72 putative acetion dehydrogenase subunit alpha YP_933043.1 1686698 D 62928 CDS YP_933044.1 119897831 4608265 1687671..1688627 1 NC_008702.1 Probable acetoin dehydrogenase, beta subunit. Homology to acoB of A. eutrophus of 41% (sprot|ACOB_ALCEU) Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme. Pfam: Transketolase, pyridine binding; Transketolase, C-terminal domain no signal peptide no TMHs; High confidence in function and specificity; acetoin dehydrogenase subunit beta 1688627 acoB1 4608265 acoB1 Azoarcus sp. BH72 acetoin dehydrogenase subunit beta YP_933044.1 1687671 D 62928 CDS YP_933045.1 119897832 4608267 1688741..1689355 1 NC_008702.1 Putative glutathione transferase. Homology to gst of E. coli of 36% (sprot|GT_ECOLI). Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. InterPro: Glutathione S-transferase C terminus (IPR004046); Glutathione S-transferase N terminus (IPR004045) Pfam: Glutathione S-transferase, N-terminal; Glutathione S-transferase, C-terminal no signal peptide no TMHs; High confidence in function and specificity; putative glutathione transferase 1689355 gst 4608267 gst Azoarcus sp. BH72 putative glutathione transferase YP_933045.1 1688741 D 62928 CDS YP_933046.1 119897833 4608956 complement(1689373..1690617) 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. Homolog to E. coli ynfM, an hypothetical transport protein,probably involved in antibiotic resistance. InterPro: General substrate transporters 2A0108: nitrate transporter; High confidence in function and specificity; putative membrane transport protein 1690617 4608956 azo1542 Azoarcus sp. BH72 putative membrane transport protein YP_933046.1 1689373 R 62928 CDS YP_933047.1 119897834 4606909 1690740..1691627 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 1691627 4606909 azo1543 Azoarcus sp. BH72 LysR family transcriptional regulator YP_933047.1 1690740 D 62928 CDS YP_933048.1 119897835 4606910 1691763..1694249 1 NC_008702.1 GGDEF/EAL/PAC/PAS-domain containing protein, sym; pNGR234a) / TREMBL: trembl|Q88A61 (38% Pseudomonas syringae (pv. tomato), sensory box/ggdef domain/eal domain protein pspto0536) InterPro: IPR000160 GGDEF. IPR000014 PAS. IPR000700 PAS-assoc_C. Pfam: PF00990 GGDEF domain. PF00989 PAS domain. PF00785 PAC motif. PF00563 EAL domain. TIGRFAM:TIGR00229 PAS domain S-box. TIGR00254 putative diguanylate cyclase (GGDEF) domain.; Conserved hypothetical protein; diguanylate cyclase 1694249 4606910 azo1544 Azoarcus sp. BH72 diguanylate cyclase YP_933048.1 1691763 D 62928 CDS YP_933049.1 119897836 4606911 1694263..1695135 1 NC_008702.1 Hypotheical protein. No good homology of the entire protein with the data base. No domains predicted. No THs. No signal peptide.; hypothetical protein 1695135 4606911 azo1545 Azoarcus sp. BH72 hypothetical protein YP_933049.1 1694263 D 62928 CDS YP_933050.1 119897837 4606912 complement(1695143..1696138) 1 NC_008702.1 Probable Citrate lyase beta chain(EC 4.1.3.6)(Citrase)(Citryl-CoA lyase subunit)(EC 4.1.3.34). In citrate-utilising prokaryotes, citrate lyase cleaves intracellular citrate into acetate and oxaloacetate, and is organised as a functional complex consisting of alpha,beta, and gamma subunits.The alpha subunit substitutes citryl for the acetyl group to form citryl-S-ACP. The beta subunit completes the reaction by cleaving the citryl to yield oxaloacetate and (regenerated) acetyl-S-ACP. Similar to the CitE protein from L.mesenteroides where is part of a gene cluster citCDEFG. SWISSPROT:CILB_LEUMC.O53078. InterPro:IPR006475; CitE.IPR001465; Malate_synthase. TIGRFAMs:TIGR01588; citE; 1.; Function unclear; putative citrate lyase subunit beta 1696138 citE 4606912 citE Azoarcus sp. BH72 putative citrate lyase subunit beta YP_933050.1 1695143 R 62928 CDS YP_933051.1 119897838 4608485 complement(1696246..1697238) 1 NC_008702.1 catalyzes the oxidation of malate to oxaloacetate; malate dehydrogenase 1697238 mdh 4608485 mdh Azoarcus sp. BH72 malate dehydrogenase YP_933051.1 1696246 R 62928 CDS YP_933052.1 119897839 4609206 1697454..1698182 1 NC_008702.1 Putative GntR-family transcriptional regulator,; Family membership; GntR family transcriptional regulator 1698182 frlR 4609206 frlR Azoarcus sp. BH72 GntR family transcriptional regulator YP_933052.1 1697454 D 62928 CDS YP_933053.1 119897840 4608829 1698280..1698669 1 NC_008702.1 Succinate dehydrogenase cytochrome b-556 subunit,sdhC.58% Identity to TrEMBL;Q7NZ57.52% to TrEMBL;Q9JRJ0,36% to SwissProt;P10446. Has PF01127,Succinate dehydrogenase cytochrome b subunit;IPR000701 Sdh_cyt; Succinate dehydrogenase (SDH) is a membrane-bound complex of two main components: a membrane-extrinsic component composed of an FAD-binding flavoprotein and an iron-sulphur protein, and a hydrophobic component composed of a cytochrome b and a membrane anchor protein.The cytochrome b component is a mono heme transmembrane protein belonging to a family that includes cytochrome b-556 from bacterial SDH (gene sdhC); cytochrome b560 from the mammalian mitochondrial SDH complex and that encoded in the mitochondrial genome of some algae and in the plant Marchantia polymorpha; cytochrome b from yeast mitochondrial SDH complex (gene SDH3 or CYB3); and protein cyt-1 from Caenorhabditis. These cytochromes are proteins of about 130 residues that comprise three transmembrane regions. There are two conserved histidines which may be involved in binding the heme group.; succinate dehydrogenase 1698669 sdhC 4608829 sdhC Azoarcus sp. BH72 succinate dehydrogenase YP_933053.1 1698280 D 62928 CDS YP_933054.1 119897841 4609855 1698666..1699010 1 NC_008702.1 Succinate dehydrogenase, hydrophobic membrane anchor subunit. Homology to sdhD of C. burnetii of 41% (sprot|DHSD_COXBU). THIS IS THE HYDROPHOBIC COMPONENT OF THE SUCCINATE DEHYDROGENASE COMPLEX. IT IS SUGGESTED THAT IT IS REQUIRED TO ANCHOR THE CATALYTIC COMPONENTS TO THE CYTOPLASMIC MEMBRANE. no signal peptide 3 TMHS; Family membership; succinate dehydrogenase, hydrophobic membrane anchor subunit 1699010 sdhD 4609855 sdhD Azoarcus sp. BH72 succinate dehydrogenase, hydrophobic membrane anchor subunit YP_933054.1 1698666 D 62928 CDS YP_933055.1 119897842 4609856 1699013..1700806 1 NC_008702.1 Probable succinate dehydrogenase, flavoprotein subunit. Homology to sdhA of C. burnetii of 54% (sprot|DHSA_COXBU) Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein,cytochrome b-556 and a hydrophobic protein. CATALYTIC ACTIVITY: Succinate + acceptor = fumarate + reduced acceptor. InterPro: Fumarate reductase/succinate dehydrogenase flavoprotein N-terminal (IPR003953); Fumarate reductase/succinate dehydrogenase FAD-binding site (IPR003952); Fumarate reductase/succinate dehydrogenase, C-terminal (IPR004112) Pfam: FAD-binding domain; Fumarate reductase/succinate dehydrogenase no TMHs no signal peptide gidA: glucose-inhibited division prot; High confidence in function and specificity; succinate dehydrogenase, flavoprotein subunit 1700806 sdhA 4609856 sdhA Azoarcus sp. BH72 succinate dehydrogenase, flavoprotein subunit YP_933055.1 1699013 D 62928 CDS YP_933056.1 119897843 4609853 1700826..1701536 1 NC_008702.1 part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase; succinate dehydrogenase iron-sulfur subunit 1701536 sdhB 4609853 sdhB Azoarcus sp. BH72 succinate dehydrogenase iron-sulfur subunit YP_933056.1 1700826 D 62928 CDS YP_933057.1 119897844 4609854 1701539..1701775 1 NC_008702.1 Conserved hypothetical protein, 39% identity to Swissprot;Q46825, ygfY of Escherichia coli. Has PF03937:(IPR005631, DUF339)TPR repeat region;This family represents a set of three divergent TPR repeats found in a small group of uncharacterised proteins. No Signal Peptide or TMH present.; Family membership; hypothetical protein 1701775 4609854 azo1553 Azoarcus sp. BH72 hypothetical protein YP_933057.1 1701539 D 62928 CDS YP_933058.1 119897845 4606913 1701845..1703140 1 NC_008702.1 Probable citrate synthase. Homology to gltA of s. meliloti of 69% (sprot|CISY_RHIME). Citrate synthase is a member of a small family of enzymes that can directly form a carbon-carbon bond without the presence of metal ion cofactors. It catalyses the first reaction in the Krebs' cycle: Citrate + CoA = acetyl-CoA + H2 O + oxaloacetate InterPro: Citrate synthase (IPR002020) Pfam: Citrate synthase no signal peptide no TMHs; High confidence in function and specificity; citrate synthase 1703140 gltA 4606913 gltA Azoarcus sp. BH72 citrate synthase YP_933058.1 1701845 D 62928 CDS YP_933059.1 119897846 4608914 1703300..1706131 1 NC_008702.1 SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide; 2-oxoglutarate dehydrogenase E1 component 1706131 sucA 4608914 sucA Azoarcus sp. BH72 2-oxoglutarate dehydrogenase E1 component YP_933059.1 1703300 D 62928 CDS YP_933060.1 119897847 4609436 1706183..1707385 1 NC_008702.1 component of 2-oxoglutarate dehydrogenase complex; catalyzes the transfer of succinyl coenzyme A to form succinyl CoA as part of the conversion of 2-oxoglutarate to succinyl-CoA; dihydrolipoamide succinyltransferase 1707385 odhB 4609436 odhB Azoarcus sp. BH72 dihydrolipoamide succinyltransferase YP_933060.1 1706183 D 62928 CDS YP_933061.1 119897848 4609437 1707467..1708891 1 NC_008702.1 E3 component of 2-oxoglutarate dehydrogenase complex; catalyzes the oxidation of dihydrolipoamide to lipoamide; dihydrolipoamide dehydrogenase 1708891 odhL 4609437 odhL Azoarcus sp. BH72 dihydrolipoamide dehydrogenase YP_933061.1 1707467 D 62928 CDS YP_933062.1 119897849 4609438 complement(1708972..1711326) 1 NC_008702.1 conserved hypothetical protein but a part of protein have similarity to potassium-transporting ATPase,A subunit. TIGR Cellular role(s):Transport and binding proteins: Cations Entry name :- SWISSPROT:YACK_RHIME Prim. accession # Q9X447 Identity:-46% interPro :-IPR004623; K_ATPaseA. Pfam PF03814; KdpA; 1. Number of predicted TMHs: 0 Signal peptide probability: 0.082; hypothetical protein 1711326 4609438 azo1558 Azoarcus sp. BH72 hypothetical protein YP_933062.1 1708972 R 62928 CDS YP_933063.1 119897850 4606914 1711473..1712678 1 NC_008702.1 Hypothetical protein yhcM. TREMBL:Q8XZX3: 54% identity, 69% similarity This family of proteins contains a P-loop motif and are predicted to be ATPases InterPro:IPR005654; AFG1_ATPase. Pfam PF03969; AFG1_ATPase No signal peptide No transmembrane helices L9: ribosomal protein L9; Specificity unclear; ATPase 1712678 afg 4606914 afg Azoarcus sp. BH72 ATPase YP_933063.1 1711473 D 62928 CDS YP_933064.1 119897851 4608284 1712686..1714659 1 NC_008702.1 Probable ATP-dependent helicase,; Family membership; ATP-dependent helicase 1714659 yoaA 4608284 yoaA Azoarcus sp. BH72 ATP-dependent helicase YP_933064.1 1712686 D 62928 CDS YP_933065.1 119897852 4610194 1714770..1715264 1 NC_008702.1 Molybdopterin-guanine dinucleotide biosynthesis protein B. may bind the guanine nucleotide required for the synthesis of molybdopterin guanine dinucleotide. mobB: molybdopterin-guanine dinucleotide; High confidence in function and specificity; molybdopterin-guanine dinucleotide biosynthesis protein B 1715264 mobB1 4610194 mobB1 Azoarcus sp. BH72 molybdopterin-guanine dinucleotide biosynthesis protein B YP_933065.1 1714770 D 62928 CDS YP_933066.1 119897853 4609246 1715261..1716466 1 NC_008702.1 Molybdopterin biosynthesis protein moeA. Involved in the biosynthesis of a demolybdo-cofactor (molybdopterin) necessary for molybdo-enzymes. Seems to be involved in a step of activation of molybdenum in the form of thiomolybdenum. InterPro: Molybdenum cofactor biosynthesis protein molyb_syn: molybdenum cofactor synthesis; High confidence in function and specificity; molybdopterin cofactor biosynthesis protein 1716466 moeA1 4609246 moeA1 Azoarcus sp. BH72 molybdopterin cofactor biosynthesis protein YP_933066.1 1715261 D 62928 CDS YP_933067.1 119897854 4609257 1716479..1716733 1 NC_008702.1 Molybdopterin converting factor subunit 1 (MPT synthase subunit 1) (Molybdopterin synthase subunit 1) (Molybdenum cofactor biosynthesis protein D) . Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur atoms are provided by the active form of the small subunit whose activation involves the acquisition of sulfur and the activity of moeB/chlN.; High confidence in function and specificity; molybdopterin converting factor subunit 1716733 moaD 4609257 moaD Azoarcus sp. BH72 molybdopterin converting factor subunit YP_933067.1 1716479 D 62928 CDS YP_933068.1 119897855 4609243 1716738..1717208 1 NC_008702.1 Molybdopterin converting factor subunit 2 (MPT synthase subunit 2) (Molybdopterin synthase subunit 2) . Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group.; High confidence in function and specificity; molybdenum cofactor biosynthesis protein E 1717208 moaE 4609243 moaE Azoarcus sp. BH72 molybdenum cofactor biosynthesis protein E YP_933068.1 1716738 D 62928 CDS YP_933069.1 119897856 4609244 complement(1717276..1717779) 1 NC_008702.1 Putative phasin protein PhaP.A family of small proteins found associated with inclusions in bacterial cells. Most associate with polyhydroxyalkanoate (PHA) inclusions, the most common of which consist of polyhydroxybutyrate (PHB). These are designated granule-associate proteins or phasins. However, the member from Magnetospirillum sp. AMB-1 is called a magnetic particle membrane-specific GTPase. TrEMBL: Q93QF2 InterPro:IPR010127,TIGR01841 NO TMH Present No Signal Peptide Present; Family membership; putative phasin protein 1717779 phaP 4609244 phaP Azoarcus sp. BH72 putative phasin protein YP_933069.1 1717276 R 62928 CDS YP_933070.1 119897857 4609537 1718010..1720592 1 NC_008702.1 Probable ATP-dependent Clp protease, ATP-binding subunit ClpB. Homology to clpB of E. coli of 66% (sprot|CLPB_ECOLI). The protein is thought to be subunits of ATP-dependent proteases which act as chaperones to target the proteases to substrates. Pfam: ATPase family assocciated with various cellular activities; CPL amino terminal domain no signal peptide no TMHs; High confidence in function and specificity; ATP-dependent Clp protease ATP-binding subunit ClpB 1720592 clpB1 4609537 clpB1 Azoarcus sp. BH72 ATP-dependent Clp protease ATP-binding subunit ClpB YP_933070.1 1718010 D 62928 CDS YP_933071.1 119897858 4608487 complement(1720639..1723911) 1 NC_008702.1 GGDEF/EAL-domain containing protein; diguanylate cyclase 1723911 4608487 azo1567 Azoarcus sp. BH72 diguanylate cyclase YP_933071.1 1720639 R 62928 CDS YP_933072.1 119897859 4606915 1724061..1725956 1 NC_008702.1 Putative peptidyl-prolyl cis-trans isomerase. Homology to ppiD of E. coli of 23% (sprot|PPID_ECOLI). PPIASES ACCELERATE THE FOLDING OF PROTEINS. SEEMS TO BE INVOLVED IN THE FOLDING OF OUTER MEMBRANE PROTEINS. InterPro: PpiC-type peptidyl-prolyl cis-trans isomerase (IPR000297) Pfam: PPIC-type PPIASE domain probable 1 TMH no signal peptide; Family membership; putative peptidyl-prolyl cis-trans isomerase 1725956 ppiD 4606915 ppiD Azoarcus sp. BH72 putative peptidyl-prolyl cis-trans isomerase YP_933072.1 1724061 D 62928 CDS YP_933073.1 119897860 4609611 complement(1726037..1726828) 1 NC_008702.1 CATALYTIC ACTIVITY: Acyl-[acyl-carrier protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH Entry name:- SWISSPROT:FABI_PSEAE Prim. accession # Q9ZFE4 Identities = 173/260 (66%) InterPro :- IPR002198; ADH_short. Pfam:- PF00106; adh_short; 1. Prediction: Non-secretory protein Signal peptide probability: 0.044 Number of predicted TMHs: 0; High confidence in function and specificity; enoyl-acyl carrier protein reductase 1726828 fabI1 4609611 fabI1 Azoarcus sp. BH72 enoyl-acyl carrier protein reductase YP_933073.1 1726037 R 62928 CDS YP_933074.1 119897861 4608714 1726995..1727903 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RSC3414 of Ralstonia solanacearum of 48% (sprot|YY14_RALSO(SRS)), Has PF05249:(IPR007913);Uncharacterised protein family (UPF0187);This family of proteins is functionally uncharacterised. signal peptide 2 TMHs; Conserved hypothetical protein; hypothetical protein 1727903 4608714 azo1570 Azoarcus sp. BH72 hypothetical protein YP_933074.1 1726995 D 62928 CDS YP_933075.1 119897862 4606916 1727944..1730145 1 NC_008702.1 Conserved hypothetical protein. Homology to hbpA of N. europaea of 56%. Protein consist of two domains: 1. bacterial extracellular sloute protein with homology to heme-binding protein A which is important for the heme aquisition and 2. a transmembrane helix a the C-terminus (unusual combination). Interpro: Bacterial extracellular solute-binding protein, family 5 (IPR000914) Pfam: Bacterial extracellular solute binding protein Signal peptide one TMH; hypothetical protein 1730145 4606916 azo1571 Azoarcus sp. BH72 hypothetical protein YP_933075.1 1727944 D 62928 CDS YP_933076.1 119897863 4606917 1730147..1731124 1 NC_008702.1 Dipeptide transport system permease dppB. Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the translocation of the substrate across the membrane. InterPro: Binding-protein-dependent transport systems inner membrane component crcB: crcB protein; High confidence in function and specificity; putative oligopeptide ABC transporter permease 1731124 appB 4606917 appB Azoarcus sp. BH72 putative oligopeptide ABC transporter permease YP_933076.1 1730147 D 62928 CDS YP_933077.1 119897864 4608328 1731121..1732533 1 NC_008702.1 Oligopeptide transport system permease appC. This protein is a component of an oligopeptide permease a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence though unlike OPP is incapable of transporting tripeptides. Probably responsible for the translocation of the substrate across the membrane (By similarity). pts-Glc: PTS system maltose and gluco; High confidence in function and specificity; oligopeptide ABC transporter permease 1732533 appC 4608328 appC Azoarcus sp. BH72 oligopeptide ABC transporter permease YP_933077.1 1731121 D 62928 CDS YP_933078.1 119897865 4608329 1732640..1734199 1 NC_008702.1 Conserved hypothetical protein. Homology to XCC2284 of X.campestris of 67% (tremble:Q8P8F8) Has PF02696;Uncharacterized ACR, YdiU/UPF0061 family(Protein of Unknown Function), IPR003846. No signal peptide. No TMHs; hypothetical protein 1734199 4608329 azo1574 Azoarcus sp. BH72 hypothetical protein YP_933078.1 1732640 D 62928 CDS YP_933079.1 119897866 4606918 complement(1734206..1734787) 1 NC_008702.1 Probable NnrU protein, 31% identity to TrEMBL;Q92Z13, Q9AAA0 Has PF07298(IPR009915):NnrU protein;This family consists of several plant and bacterial NnrU proteins. NnrU is thought to be involved in the reduction of nitric oxide. The exact function of NnrU is unclear. It is thought however that NnrU and perhaps NnrT are required for expression of both nirK and nor; NnrU family protein 1734787 nnrU 4606918 nnrU Azoarcus sp. BH72 NnrU family protein YP_933079.1 1734206 R 62928 CDS YP_933080.1 119897867 4609388 complement(1734888..1735334) 1 NC_008702.1 binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation; SsrA-binding protein 1735334 smpB 4609388 smpB Azoarcus sp. BH72 SsrA-binding protein YP_933080.1 1734888 R 62928 CDS YP_933081.1 119897868 4609877 1735447..1735884 1 NC_008702.1 Entry name TREMBL:Q8XZH0 Prim. accession # Q8XZH0 InterPro :- IPR005340; UPF0083. Pfam PF03654; ARPF; 1. Identities = 78/142 (54%), Number of predicted TMHs: 0 Prediction: Non-secretory protein Signal peptide probability: 0.000; hypothetical protein 1735884 4609877 azo1577 Azoarcus sp. BH72 hypothetical protein YP_933081.1 1735447 D 62928 CDS YP_933082.1 119897869 4606919 1735881..1736204 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3462 of C.violaceum of 54% (tremble:Q7NSG4) Has PF03658;Uncharacterised protein family (UPF0125). IPR005346 Has no signal peptide or TMH present.; hypothetical protein 1736204 4606919 azo1578 Azoarcus sp. BH72 hypothetical protein YP_933082.1 1735881 D 62928 CDS YP_933083.1 119897870 4606920 complement(1736223..1736780) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS05265 of R. solanacearum of 30% (trembl|Q8XZG8(SRS)) No domains predicted Signal peptide present No TMH reported.; Conserved hypothetical protein; hypothetical protein 1736780 4606920 azo1579 Azoarcus sp. BH72 hypothetical protein YP_933083.1 1736223 R 62928 CDS YP_933084.1 119897871 4606921 1736897..1738357 1 NC_008702.1 IMP dehydrogenase (Inosine-5'-monophosphate dehydrogenase) (Inosinic acid dehydrogenase) (Inosinate dehydrogenase) (IMP oxidoreductase) (Inosine monophosphate oxidoreductase) kpsF: KpsF/GutQ family protein; High confidence in function and specificity; IMP dehydrogenase 1738357 guaB 4606921 guaB Azoarcus sp. BH72 IMP dehydrogenase YP_933084.1 1736897 D 62928 CDS YP_933085.1 119897872 4608962 1738436..1740001 1 NC_008702.1 contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway; GMP synthase 1740001 guaA 4608962 guaA Azoarcus sp. BH72 GMP synthase YP_933085.1 1738436 D 62928 CDS YP_933086.1 119897873 4608961 complement(1740086..1740970) 1 NC_008702.1 HTH-type transcriptional activator,; Specificity unclear; HTH-type transcriptional activator 1740970 4608961 azo1582 Azoarcus sp. BH72 HTH-type transcriptional activator YP_933086.1 1740086 R 62928 CDS YP_933087.1 119897874 4606922 1741067..1741870 1 NC_008702.1 NAD(P)H dehydrogenase [quinone] 1 (EC 1.6.5.2) (Quinone reductase 1) (QR1) (DT-diaphorase) (DTD) (Azoreductase) (Phylloquinone reductase) (Menadione reductase). The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis (By similarity). trembl:Q9I4B4:72%identity; 81% similarity InterPro: NAD(P)H dehydrogenase (quinone) InterPro:IPR003680; NADHdh_2. Pfam:PF02525; Flavodoxin_2 crcB: crcB protein No signal peptide present Absence of transmembrane helices (TMHMM predicted); High confidence in function and specificity; NAD(P)H dehydrogenase, quinone 1 1741870 nqo1 4606922 nqo1 Azoarcus sp. BH72 NAD(P)H dehydrogenase, quinone 1 YP_933087.1 1741067 D 62928 CDS YP_933088.1 119897875 4609410 complement(1741890..1743509) 1 NC_008702.1 Putative two-component hybrid sensor and regulator ,; Function unclear; putative two-component hybrid sensor and regulator 1743509 4609410 azo1584 Azoarcus sp. BH72 putative two-component hybrid sensor and regulator YP_933088.1 1741890 R 62928 CDS YP_933089.1 119897876 4606923 complement(1743693..1744202) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. signal peptide. 1 TMH; hypothetical protein 1744202 4606923 azo1585 Azoarcus sp. BH72 hypothetical protein YP_933089.1 1743693 R 62928 CDS YP_933090.1 119897877 4606924 complement(1744406..1744909) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Avin02003713 of Azotobacter vinelandii of 35% (gi|53610668|ref|ZP_00089587.2|(NBCI ENTREZ)). No domains predicted. Signal P reporting signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 1744909 4606924 azo1586 Azoarcus sp. BH72 hypothetical protein YP_933090.1 1744406 R 62928 CDS YP_933091.1 119897878 4606925 complement(1744963..1746744) 1 NC_008702.1 Putative two-component system sensor protein,; Function unclear; putative two-component system sensor protein 1746744 4606925 azo1587 Azoarcus sp. BH72 putative two-component system sensor protein YP_933091.1 1744963 R 62928 CDS YP_933092.1 119897879 4606926 1746847..1747311 1 NC_008702.1 Cytidine deaminase (EC: 3.5.4.5) (cytidine aminohydrolase) catalyzes the hydrolysis of cytidine into uridine and ammonia while deoxycytidylate deaminase (EC: 3.5.4.12) (dCMP deaminase) hydrolyzes dCMP into dUMP. Both enzymes are known to bind zinc and to require it for their catalytic activity [1, 2]. These two enzymes do not share any sequence similarity with the exception of a region that contains three conserved histidine and cysteine residues which are thought to be involved in the binding of the catalytic zinc ion.; High confidence in function and specificity; cytidine deaminase 1747311 cumB 4606926 cumB Azoarcus sp. BH72 cytidine deaminase YP_933092.1 1746847 D 62928 CDS YP_933093.1 119897880 4608542 1747313..1747792 1 NC_008702.1 Conserved hypothetical protein. Homology to CBU1080 of C.burnetii of 59% (tremble:Q83CN1) Has PF03734;ErfK/YbiS/YcfS/YnhG;(IPR005490)This family of proteins are found in a range of bacteria. Th conserved region contains a conserved histidine and cysteine,suggesting that these proteins have an enzymatic activity. Several members of this family contain peptidoglycan binding domains. So these proteins may use peptidoglycan or a precursor as a substrate. No TMHs. No signal peptide.; hypothetical protein 1747792 4608542 azo1589 Azoarcus sp. BH72 hypothetical protein YP_933093.1 1747313 D 62928 CDS YP_933094.1 119897881 4606927 1747789..1748220 1 NC_008702.1 Conserved hypothetical acetyltransferase. Homology to glr3404 of G. violaceus of 52% (trembl|Q7NFX1). InterPro: GCN5-related N-acetyltransferase (IPR000182) Pfam: Acetyltransferase (GNAT) family no signal peptide no TMHS; Family membership; acetyltransferase 1748220 4606927 azo1590 Azoarcus sp. BH72 acetyltransferase YP_933094.1 1747789 D 62928 CDS YP_933095.1 119897882 4606928 complement(1748232..1748999) 1 NC_008702.1 Conserved hypothetical protein. Homology to GSU0792 of G.sulfurreducens of 50% (gsu:GSU0792(KEGG)) Has PF05742(IPR008551);Protein of unknown function (DUF833);This family is found in eukaryotes, prokaryotes and viruses and has no known function. P54797 has been found to be expressed during early embryogenesis in mice. No signal peptide or TMH present.; hypothetical protein 1748999 4606928 azo1591 Azoarcus sp. BH72 hypothetical protein YP_933095.1 1748232 R 62928 CDS YP_933096.1 119897883 4606929 complement(1748990..1749307) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD92 of Azoarcus sp. EbN1 of 46% (gnl|keqq|eba:ebD92(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1749307 4606929 azo1592 Azoarcus sp. BH72 hypothetical protein YP_933096.1 1748990 R 62928 CDS YP_933097.1 119897884 4606930 complement(1749321..1750349) 1 NC_008702.1 Conserved hypothetical protein aminomethyltransferase . Homology to ne1514 of N. europaea of 37% (trembl|Q82UH2) InterPro: Glycine cleavage T-protein (aminomethyl transferase) Tigrfam: gcvT: glycine cleavage system T protein Pfam: Glycine cleavage T-protein This is a family of glycine cleavage T-proteins, part of the glycine cleavage multienzyme complex (GCV) which catalyses the catabolism of glycine. The T-protein is an aminomethyl transferase that catalyses the following reaction: (6S)-tetrahydrofolate + S-aminomethyldihydrolipoylprotein = (6R)-5,10-methylenetetrahydrofolate + NH3 + dihydrolipoylprotein; Specificity unclear; aminomethyltransferase 1750349 4606930 azo1593 Azoarcus sp. BH72 aminomethyltransferase YP_933097.1 1749321 R 62928 CDS YP_933098.1 119897885 4606931 1750580..1751584 1 NC_008702.1 Hypothetical protein HI0457. This entry describes proteins of unknown function TREMBL:Q8XYH5: 50% identity,66% similarity InterPro:IPR003770; DUF175. Pfam:PF02618; ADC_lyas Non secretory protein with signal peptide probability 0.977 (Signal P predicted) No. of transmembrane helices: 1 (TMHMM predicted) This entry describes proteins of unknown function 2A0108: nitrate transporter; Function unclear; hypothetical protein 1751584 4606931 azo1594 Azoarcus sp. BH72 hypothetical protein YP_933098.1 1750580 D 62928 CDS YP_933099.1 119897886 4606932 1751590..1752210 1 NC_008702.1 catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP); thymidylate kinase 1752210 tmk 4606932 tmk Azoarcus sp. BH72 thymidylate kinase YP_933099.1 1751590 D 62928 CDS YP_933100.1 119897887 4609953 1752207..1753256 1 NC_008702.1 DNA polymerase III delta subunit. DNA polymerase III is a complex multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3 to 5 exonuclease activity (By similarity). TIGRFAM: holB: DNA polymerase III delta prime.; High confidence in function and specificity; DNA-directed DNA polymerase 1753256 holB 4609953 holB Azoarcus sp. BH72 DNA-directed DNA polymerase YP_933100.1 1752207 D 62928 CDS YP_933101.1 119897888 4609013 1753253..1753624 1 NC_008702.1 Type IV pilus biogenesis protein PilZ, 56% identity to TrEMBL;Q87YH3, Q8PBU4. Has PF07238, Type IV pilus assembly protein PilZ; IPR009875 ;This family consists of several bacterial type IV pilus assembly proteins. PilZ is thought to have a cytoplasmic location and be essential for type 4 fimbrial biogenesis but its exact function is unknown.; type IV pilus assembly protein PilZ 1753624 pilZ 4609013 pilZ Azoarcus sp. BH72 type IV pilus assembly protein PilZ YP_933101.1 1753253 D 62928 CDS YP_933102.1 119897889 4609581 1753650..1754420 1 NC_008702.1 Putative deoxyribonuclease yabD (EC 3.1.21.-). InterPro: Uncharacterized protein family UPF0006. TIGR00010: deoxyribonuclease TatD family.; Specificity unclear; putative deoxyribonuclease 1754420 4609581 azo1598 Azoarcus sp. BH72 putative deoxyribonuclease YP_933102.1 1753650 D 62928 CDS YP_933103.1 119897890 4606933 1754407..1755096 1 NC_008702.1 Hypothetical protein KIAA1223 (Fragment). TREMBL:Q8XYH0:35% identity; TREMBL:Q7VXC9:35% The ankyrin repeat is one of the most common protein-protein interaction motifs in nature. Ankyrin repeats are tandemly repeated modules of about 33 amino acids. They occur in a large number of functionally diverse proteins mainly from eukaryotes.Each Ankyrin repeat folds into a helix-loop-helix structure with a beta-hairpin/loop region projecting out from the helices at a 90 angle. The repeats stack together to form an L-shaped structure InterPro: Ankyrin-repeat Pfam:Phage_integr_N:Phage integrase, N-terminal SAM-like wecB_tagA_cpsF: glycosyl transferase. Transmembrane helices 0 and presence of signal peptide (Signal P)for exporting.(probably signal peptide bearing exported protein); Specificity unclear; ankyrin repeat-containing protein 1755096 ank1 4606933 ank1 Azoarcus sp. BH72 ankyrin repeat-containing protein YP_933103.1 1754407 D 62928 CDS YP_933104.1 119897891 4608320 complement(1755103..1755936) 1 NC_008702.1 Conserved hypothetical protein. Homology to Rgel02003216 of Rubrivivax gelatinosus of 54% (gi|47572589|ref|ZP_00242632.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 1755936 4608320 azo1600 Azoarcus sp. BH72 hypothetical protein YP_933104.1 1755103 R 62928 CDS YP_933105.1 119897892 4606934 complement(1755933..1756850) 1 NC_008702.1 Histone deacetylases catalyse the removal of the acetyl group from histones. Histone deacetylase, acetoin utilization proteins and acetylpolyamine amidohydrolases are all members of an ancient family, TREMBL:Q7NVL6 (58% identity); TREMBL:Q8XZ00 (57% identity). InterPro (IPR000286): Histone deacetylase superfamily Pfam (PF00850): Histone deacetylase domain.; Family membership; histone deacetylase family protein 1756850 4606934 azo1601 Azoarcus sp. BH72 histone deacetylase family protein YP_933105.1 1755933 R 62928 CDS YP_933106.1 119897893 4606935 complement(1756888..1757868) 1 NC_008702.1 Conserved hypothetical protein. Homology to Rv2569c/MT2645/Mb2599c of M. bovis of 38%. Pfam: Transglutaminase-like superfamily. no signal peptide no TMHs; Specificity unclear; hypothetical protein 1757868 4606935 azo1602 Azoarcus sp. BH72 hypothetical protein YP_933106.1 1756888 R 62928 CDS YP_933107.1 119897894 4606936 complement(1757930..1760416) 1 NC_008702.1 Conserved hypothetical protein,52% Identity to TrEMBL;Q9HUN7. Has PF04169;Domain of unknown function (DUF404);(IPR007297)This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF403 (IPR007296) and DUF407 (IPR007302). Has PF04174;Domain of unknown function (DUF407);This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF403 (IPR007296) and DUF404 (IPR007297). Has PF04168;Bacterial domain of unknown function (DUF403);This is a protein of unknown function. It sometimes occurs in combination with two domains of unknown function DUF404 (IPR007297) and DUF407 (IPR007302).; Specificity unclear; hypothetical protein 1760416 4606936 azo1603 Azoarcus sp. BH72 hypothetical protein YP_933107.1 1757930 R 62928 CDS YP_933108.1 119897895 4606937 complement(1760466..1763846) 1 NC_008702.1 Transglutaminase-like superfamily domain protein;67% identity (79% similarity) to TrEMBL;Q87VL0. TrEMBL;Q6N3E0(57% identity). Has SMART;SM00460,TGc;Transglutaminase/protease-like homologues: (IPR002931):Transglutaminases are enzymes that establish covalent links between proteins. A subset of transglutaminase homologues appear to catalyse the reverse reaction, the hydrolysis of peptide bonds. Proteins with this domain are both extracellular and intracellular, and it is likely that the eukaryotic intracellular proteins are involved in signalling events. NO signal peptide or TMH present.; High confidence in function and specificity; hypothetical protein 1763846 4606937 azo1604 Azoarcus sp. BH72 hypothetical protein YP_933108.1 1760466 R 62928 CDS YP_933109.1 119897896 4606938 1764095..1765264 1 NC_008702.1 Hippurate hydrolase (EC 3.5.1.32) (Benzoylglycine amidohydrolase) (Hippuricase). TREMBL:Q7WFU4: 48% identity, 58% similarity InterPro:IPR002933; Peptidase_M20. IPR010168; Pept_M20D_amidh. Pfam: PF01546; Peptidase_M20; 1. TIGRFAMs TIGR01891; amidohydrolases No signal peptide present No transmembrane helices present mreB: cell shape determining protein M; High confidence in function and specificity; hippurate hydrolase 1765264 hipO2 4606938 hipO2 Azoarcus sp. BH72 hippurate hydrolase YP_933109.1 1764095 D 62928 CDS YP_933110.1 119897897 4608998 1765274..1767766 1 NC_008702.1 Similar to TREMBL:Q7WGZ1 (41% identity); TREMBL:Q7W9R7 (41% identity); TREMBL:Q8XXM2 (37% identity). Pfam (DUF214): Predicted permease. TMHMM reporting 10 transmembrane helices.; Specificity unclear; putative inner membrane transport permease 1767766 4608998 azo1606 Azoarcus sp. BH72 putative inner membrane transport permease YP_933110.1 1765274 D 62928 CDS YP_933111.1 119897898 4606939 complement(1767782..1768855) 1 NC_008702.1 together with moaC, is involved in the conversion of a guanosine derivative (GXP) into molybdopterin precursor Z; molybdenum cofactor biosynthesis protein A 1768855 moaA 4606939 moaA Azoarcus sp. BH72 molybdenum cofactor biosynthesis protein A YP_933111.1 1767782 R 62928 CDS YP_933112.1 119897899 4609241 1769067..1769345 1 NC_008702.1 hypothetical protein having 55% similarity with Nitrosomonas europaea No Signal peptide present No TMH reported.; hypothetical protein 1769345 4609241 azo1608 Azoarcus sp. BH72 hypothetical protein YP_933112.1 1769067 D 62928 CDS YP_933113.1 119897900 4606940 1769477..1771894 1 NC_008702.1 Conserved hypothetical ATP-dependent protease. Homology to gsu2433 of G. sulfurreducens of 40% (tremblnew|AAR35806). Tigrfam:lon_rel: ATP-dependent protease puta no signal peptide no TMHs; High confidence in function and specificity; ATP-dependent protease 1771894 4606940 azo1609 Azoarcus sp. BH72 ATP-dependent protease YP_933113.1 1769477 D 62928 CDS YP_933114.1 119897901 4609986 complement(1772287..1773495) 1 NC_008702.1 catalyzes the formation of L-glutamate and an aromatic oxo acid from an aromatic amino acid and 2-oxoglutarate; aromatic amino acid aminotransferase 1773495 tyrB 4609986 tyrB Azoarcus sp. BH72 aromatic amino acid aminotransferase YP_933114.1 1772287 R 62928 CDS YP_933115.1 119897902 4610055 1773639..1775714 1 NC_008702.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion; excinuclease ABC subunit B 1775714 uvrB 4610055 uvrB Azoarcus sp. BH72 excinuclease ABC subunit B YP_933115.1 1773639 D 62928 CDS YP_933116.1 119897903 4610082 1775724..1776188 1 NC_008702.1 Low molecular weight protein-tyrosine-phosphatase,; High confidence in function and specificity; PtpA protein 1776188 ptpA 4610082 ptpA Azoarcus sp. BH72 PtpA protein YP_933116.1 1775724 D 62928 CDS YP_933117.1 119897904 4609647 complement(1776283..1779327) 1 NC_008702.1 Probable ribonuclease E (EC 3.1.4.-) (RNase E). Homology to rne of E. coli of 44% (sprot|RNE_ECOLI) This protein matures 5S rRNA from its precursors from all the rRNA genes. It is the major endoribonuclease participating in mRNA turnover. Interpro: Ribonuclease E and G (IPR004659); S1 RNA binding domain (IPR003029) Pfam: S1 RNA binding domain Tigrfam: RNase EG: ribonuclease,Rne/Rng family no signal peptide no TMHs; Family membership; ribonuclease E 1779327 rne 4609647 rne Azoarcus sp. BH72 ribonuclease E YP_933117.1 1776283 R 62928 CDS YP_933118.1 119897905 4609739 1779739..1780698 1 NC_008702.1 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil at positions 955 2504 and 2580 in 23S ribosomal RNA.; Family membership; ribosomal large subunit pseudouridine synthase C 1780698 rluC 4609739 rluC Azoarcus sp. BH72 ribosomal large subunit pseudouridine synthase C YP_933118.1 1779739 D 62928 CDS YP_933119.1 119897906 4609733 1780691..1781347 1 NC_008702.1 TREMBL:Q7VSFO: Probable hydrolase, 55% identity,63% similarity Phosphoglycolate phosphatase (PGP). InterPro:PR006402; HAD-SF-IA-v3. IPR006439; HAD_SF_A_v1. IPR005834; Hydrolase. Pfam:PF00702; Hydrolase; Haloacid dehaloginase like hydrolase TIGRFAMs:TIGR01549; HAD-SF-IA-v1; TIGR01509; HAD-SF-IA-v3; serB: phosphoserine phosphatase SerB hstdl_phs_rel: histidinol phosphatase-; Function unclear; hydrolase 1781347 4609733 azo1615 Azoarcus sp. BH72 hydrolase YP_933119.1 1780691 D 62928 CDS YP_933120.1 119897907 4606942 1781326..1781682 1 NC_008702.1 Conserved hypothetical ferredoxin subunits of nitrite reductase. Conserved Rieske domain is present. Similar to NirD from E.Coli. InterPro: Rieske iron-sulfur protein 2Fe-2S subunit Non-secretory protein; Function unclear; ferredoxin protein 1781682 4606942 azo1616 Azoarcus sp. BH72 ferredoxin protein YP_933120.1 1781326 D 62928 CDS YP_933121.1 119897908 4606943 1781715..1782626 1 NC_008702.1 Conserved hypothetical peptidase. Haomology to ne1024 of N. europaea of 59% (trembl|Q82VP2) InterPro: Peptidase family U7 (IPR002142) Tigrfam: SppA_dom: signal peptide peptidase SppA Pfam: Peptidase family U7 no signal peptide probable 1 TMH; High confidence in function and specificity; peptidase 1782626 4606943 azo1617 Azoarcus sp. BH72 peptidase YP_933121.1 1781715 D 62928 CDS YP_933122.1 119897909 4606944 complement(1782623..1783348) 1 NC_008702.1 Conserved hypothetical protein. Homology to bpp3312 of B. parapertussis (trembl|Q7WD15). TIGR00096: conserved hypothetical protein Pfam: Tetrapyrrole (Corrin/Porphyrin) Methyltransferase no signal peptide no TMHs; hypothetical protein 1783348 4606944 azo1618 Azoarcus sp. BH72 hypothetical protein YP_933122.1 1782623 R 62928 CDS YP_933123.1 119897910 4606945 complement(1783345..1783923) 1 NC_008702.1 Maf-like protein yceF. InterPro: Maf-like protein; Family membership; Maf-like protein 1783923 yceF 4606945 yceF Azoarcus sp. BH72 Maf-like protein YP_933123.1 1783345 R 62928 CDS YP_933124.1 119897911 4610156 1784046..1784531 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3419 of Chromobacterium violaceum of 42% (trembl:Q7NSK4). Has PF02620, Uncharacterized ACR,IPR003772;This entry describes proteins of unknown function. No signal peptide. No TMHs; hypothetical protein 1784531 4610156 azo1620 Azoarcus sp. BH72 hypothetical protein YP_933124.1 1784046 D 62928 CDS YP_933125.1 119897912 4606946 1784569..1784748 1 NC_008702.1 some L32 proteins have zinc finger motifs consisting of CXXC while others do not; 50S ribosomal protein L32 1784748 rpmF 4606946 rpmF Azoarcus sp. BH72 50S ribosomal protein L32 YP_933125.1 1784569 D 62928 CDS YP_933126.1 119897913 4609790 1784837..1785853 1 NC_008702.1 involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY; putative glycerol-3-phosphate acyltransferase PlsX 1785853 plsX 4609790 plsX Azoarcus sp. BH72 putative glycerol-3-phosphate acyltransferase PlsX YP_933126.1 1784837 D 62928 CDS YP_933127.1 119897914 4609585 1785850..1786815 1 NC_008702.1 FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs; 3-oxoacyl-ACP synthase 1786815 fabH 4609585 fabH Azoarcus sp. BH72 3-oxoacyl-ACP synthase YP_933127.1 1785850 D 62928 CDS YP_933128.1 119897915 4608713 1786843..1787772 1 NC_008702.1 Probable malonyl CoA-acyl carrier protein transacylase. Homology to fabD of E. coli of 54% (sprot|FABD_ECOLI). Is involved in fatty acid biosynthesis and transfers the malonyl moeity from coenzyme A to acyl-carrier protein. InterPro: Acyl transferase domain (IPR001227); Manyl CoA-acyl carrier protein transaylase (IPR004410) Pfam: Acyl transferase domain Tigrfam: fabD: malonyl CoA-acyl carrier protein no signal peptide no TMHs; High confidence in function and specificity; malonyl CoA-acyl carrier protein transacylase 1787772 fabD 4608713 fabD Azoarcus sp. BH72 malonyl CoA-acyl carrier protein transacylase YP_933128.1 1786843 D 62928 CDS YP_933129.1 119897916 4608708 1787775..1788524 1 NC_008702.1 catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 1788524 fabG 4608708 fabG Azoarcus sp. BH72 3-ketoacyl-ACP reductase YP_933129.1 1787775 D 62928 CDS YP_933130.1 119897917 4608711 1788618..1788857 1 NC_008702.1 carries the fatty acid chain in fatty acid biosynthesis; acyl carrier protein 1788857 acpP 4608711 acpP Azoarcus sp. BH72 acyl carrier protein YP_933130.1 1788618 D 62928 CDS YP_933131.1 119897918 4608270 1788940..1790175 1 NC_008702.1 FabF; beta-ketoacyl-ACP synthase II, KASII; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-(acyl carrier protein) synthase II 1790175 fabF1 4608270 fabF1 Azoarcus sp. BH72 3-oxoacyl-(acyl carrier protein) synthase II YP_933131.1 1788940 D 62928 CDS YP_933132.1 119897919 4608709 1790198..1790665 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA5460 of Azoarcus sp. EbN1 of 32% (gnl|keqq|eba:ebA5460(KEGG)). No domains predicted. No signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 1790665 4608709 azo1628 Azoarcus sp. BH72 hypothetical protein YP_933132.1 1790198 D 62928 CDS YP_933133.1 119897920 4606947 complement(1790655..1792244) 1 NC_008702.1 catalyzes the formation of oxaloacetate from L-aspartate; L-aspartate oxidase 1792244 nadB 4606947 nadB Azoarcus sp. BH72 L-aspartate oxidase YP_933133.1 1790655 R 62928 CDS YP_933134.1 119897921 4609303 1792464..1793063 1 NC_008702.1 Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; this sigma factor is involved in heat shock and oxidative stress response; RNA polymerase sigma factor RpoE 1793063 algU 4609303 algU Azoarcus sp. BH72 RNA polymerase sigma factor RpoE YP_933134.1 1792464 D 62928 CDS YP_933135.1 119897922 4608299 1793073..1793618 1 NC_008702.1 Putative sigma-E factor, negative regulatory protein,; High confidence in function and specificity; putative sigma-E factor, negative regulatory protein 1793618 rseA 4608299 rseA Azoarcus sp. BH72 putative sigma-E factor, negative regulatory protein YP_933135.1 1793073 D 62928 CDS YP_933136.1 119897923 4609825 1793615..1794586 1 NC_008702.1 Putative sigma factor regulatory protein,; High confidence in function and specificity; putative sigma factor regulatory protein 1794586 rseB 4609825 rseB Azoarcus sp. BH72 putative sigma factor regulatory protein YP_933136.1 1793615 D 62928 CDS YP_933137.1 119897924 4609826 1794586..1795026 1 NC_008702.1 Putative sigma-E factor regulatory protein RseC,; High confidence in function and specificity; putative sigma-E factor regulatory protein RseC 1795026 rseC 4609826 rseC Azoarcus sp. BH72 putative sigma-E factor regulatory protein RseC YP_933137.1 1794586 D 62928 CDS YP_933138.1 119897925 4609827 1795023..1796441 1 NC_008702.1 Probable serine protease MucD. Homology to mucD of P. aruginosa of 47% (trembl|Q57155) InterPro: Serine proteases trypsin family (IPR001254); PDZ domain (also known as DHR or GLGF) (IPR001478); Chymotrypsin serin protease family (S1) (IPR001314); HtrA/DegQ protease family (IPR001940) Pfam: Trypsin; PDZ domain (also known as DHR or GLGF) signal peptide no TMHs ahpD_dom: alkylhydroperoxidase AhpD fam; High confidence in function and specificity; serine protease MucD 1796441 mucD2 4609827 mucD2 Azoarcus sp. BH72 serine protease MucD YP_933138.1 1795023 D 62928 CDS YP_933139.1 119897926 4609284 1796438..1796695 1 NC_008702.1 Conserved hypothetical thioredoxin. Homology to a putative thioredoxin of N. menigitidis (NMA0966) of 50%. Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. no signal peptide no TMHs; Function unclear; thioredoxin-disulfide reductase 1796695 4609284 azo1635 Azoarcus sp. BH72 thioredoxin-disulfide reductase YP_933139.1 1796438 D 62928 CDS YP_933140.1 119897927 4606948 complement(1796728..1797744) 1 NC_008702.1 HTH-type transcriptional regulator,; Family membership; HTH-type transcriptional regulator 1797744 4606948 azo1636 Azoarcus sp. BH72 HTH-type transcriptional regulator YP_933140.1 1796728 R 62928 CDS YP_933141.1 119897928 4606949 1797863..1798198 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide 3 TMHs; hypothetical protein 1798198 4606949 azo1637 Azoarcus sp. BH72 hypothetical protein YP_933141.1 1797863 D 62928 CDS YP_933142.1 119897929 4606950 1798230..1799195 1 NC_008702.1 Putative exported protein, 39% identity (54% similarity)to TrEMBL;Q7VUI5 TrEMBL;Q7WNJ8. Has PF03401;Bordetella uptake gene (bug) product;(IPR005064)These probable extra-cytoplasmic solute receptors are strongly overrepresented in several beta-proteobacteria. SIgnal Peptide Present. No TMH reported present.; Specificity unclear; hypothetical protein 1799195 4606950 azo1638 Azoarcus sp. BH72 hypothetical protein YP_933142.1 1798230 D 62928 CDS YP_933143.1 119897930 4606951 1799548..1801344 1 NC_008702.1 binds to the ribosome on the universally-conserved alpha-sarcin loop; GTP-binding protein LepA 1801344 lepA 4606951 lepA Azoarcus sp. BH72 GTP-binding protein LepA YP_933143.1 1799548 D 62928 CDS YP_933144.1 119897931 4609127 1801366..1802154 1 NC_008702.1 Signal peptidase I (EC 3.4.21.89) (SPase I) (Leader peptidase I). SPase I is an integral membrane protein that is anchored in the cytoplasmic membrane by one or two N-terminal transmembrane domains, with the main part of the protein protuding in the periplasmic space. SPase I can cleave N-terminal leader sequences of nonlipoprotein that are exported by the SecYEG pathway or the twin arginine translocation (Tat) pathway. Pfam: Peptidase_S26 probable 2 TMH no signal peptide; High confidence in function and specificity; LepB protein 1802154 lepB 4609127 lepB Azoarcus sp. BH72 LepB protein YP_933144.1 1801366 D 62928 CDS YP_933145.1 119897932 4609128 1802170..1802529 1 NC_008702.1 Conserved hypothetical secreted protein. Homology with NE2325 of N.europaea of 41% (tremble:Q82SJ5) No domains predicted. Signal peptide Present. No TMH reported.; Conserved hypothetical protein; hypothetical protein 1802529 4609128 azo1641 Azoarcus sp. BH72 hypothetical protein YP_933145.1 1802170 D 62928 CDS YP_933146.1 119897933 4606952 1802535..1803206 1 NC_008702.1 cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity; ribonuclease III 1803206 rnc 4606952 rnc Azoarcus sp. BH72 ribonuclease III YP_933146.1 1802535 D 62928 CDS YP_933147.1 119897934 4609738 1803203..1804117 1 NC_008702.1 Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome; GTP-binding protein Era 1804117 era 4609738 era Azoarcus sp. BH72 GTP-binding protein Era YP_933147.1 1803203 D 62928 CDS YP_933148.1 119897935 4608674 1804142..1804873 1 NC_008702.1 involved in DNA repair and RecFOR pathway recombination; RecFOR proteins displace ssDNA-binding protein and facilitate the production of RecA-coated ssDNA; DNA repair protein RecO 1804873 recO 4608674 recO Azoarcus sp. BH72 DNA repair protein RecO YP_933148.1 1804142 D 62928 CDS YP_933149.1 119897936 4609709 1804870..1805619 1 NC_008702.1 involved in the de novo synthesis of pyridoxine (Vitamin B6); pyridoxine 5'-phosphate synthase 1805619 pdxJ 4609709 pdxJ Azoarcus sp. BH72 pyridoxine 5'-phosphate synthase YP_933149.1 1804870 D 62928 CDS YP_933150.1 119897937 4609517 1805634..1806011 1 NC_008702.1 Holo-[acyl-carrier-protein] synthase, 50% Identity to TrEMBL;Q7NWB8, Q820I1. SProt;Q9KPB6(49%). Has PF01648,4'-phosphopantetheinyl transferase superfamily; IPR008278 4-PPT_transf; Members of this family transfers the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of PP-binding. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: The ACPS type such as P24224 and the Sfp type such as P39135. The structure of the Sfp type is known which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.; holo-[acyl-carrier-protein] synthase 1806011 acpS 4609517 acpS Azoarcus sp. BH72 holo-[acyl-carrier-protein] synthase YP_933150.1 1805634 D 62928 CDS YP_933151.1 119897938 4608271 1806008..1807141 1 NC_008702.1 hydrolyzes the terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides; beta-hexosaminidase 1807141 nagZ2 4608271 nagZ2 Azoarcus sp. BH72 beta-hexosaminidase YP_933151.1 1806008 D 62928 CDS YP_933152.1 119897939 4609316 1807351..1809171 1 NC_008702.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision; excinuclease ABC subunit C 1809171 uvrC 4609316 uvrC Azoarcus sp. BH72 excinuclease ABC subunit C YP_933152.1 1807351 D 62928 CDS YP_933153.1 119897940 4610083 1809267..1809839 1 NC_008702.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (PGP synthase). It catalyses the conversion of CDP-diacylglycerol and glycerol-3-phosphate to CMP and 3-(3-phosphatidyl)-glycerol 1-phosphate in the commited step to the synthesis of acidic phospholipids. It is an integral membrane protein. A number of related enzymes are quite similar in both sequence and catalytic activity, including Saccharamyces cerevisiae YDL142c, now known to be a cardiolipin synthase. Entry name :-SWISSPROT:PGSA_ECOLI Prim. accession # P06978 Identities = 50% InterPro IPR000462; CDP-OH_P_trans. IPR004570; PgsA. Pfam PF01066; CDP-OH_P_transf; 1. Number of predicted TMHs: 5 Prediction: Non-secretory protein Signal peptide probability: 0.109; Family membership; CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase 1809839 pgsA 4610083 pgsA Azoarcus sp. BH72 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase YP_933153.1 1809267 D 62928 CDS YP_933154.1 119897941 4609994 1810266..1811441 1 NC_008702.1 Probable Serine--pyruvate aminotransferase mitochondrial precursor (EC 2.6.1.51) (SPT) (Alanine--glyoxylate aminotransferase) (EC 2.6.1.44) (AGT). TREMBL:Q7P1E0:36% identity, 45% similarity This family consists of phosphodiesterases, including human plasma-cell membrane glycoprotein PC-1 / alkaline phosphodiesterase I / nucleotide pyrophosphatase (nppase). These enzymes catalyse the cleavage of phosphodiester and phosphosulphate bonds in NAD, deoxynucleotides and nucleotide sugars InterPro:IPR002591; Phosphodiest. Pfam:PF01663; Phosphodiest; bcpB: phosphonopyruvate decarboxylase-rel No transmembrane helices; Function unclear; putative phosphodiesterase 1811441 4609994 azo1650 Azoarcus sp. BH72 putative phosphodiesterase YP_933154.1 1810266 D 62928 CDS YP_933155.1 119897942 4606953 1811528..1811716 1 NC_008702.1 Hypothetical protein. No homologs in the database. Has No domains, repeats, motifs or features.; hypothetical protein 1811716 4606953 azo1651 Azoarcus sp. BH72 hypothetical protein YP_933155.1 1811528 D 62928 CDS YP_933156.1 119897943 4606954 complement(1811729..1812199) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS03611 of R.solanacearum of 40% (trembl:Q8XXR7). Has PF06676(IPR009562):Protein of unknown function (DUF1178);This family consists of several hypothetical bacterial proteins of around 150 residues in length. The function of this family is unknown. No signal peptide or TMH reported present.; hypothetical protein 1812199 4606954 azo1652 Azoarcus sp. BH72 hypothetical protein YP_933156.1 1811729 R 62928 CDS YP_933157.1 119897944 4610006 1812617..1815325 1 NC_008702.1 Putative Autotransporter30% identity to TrEMBL;Q87UU2. Weak homology with hits in the DB. Signal Peptide present. No TMH reported present. Has PF03797;Autotransporter beta-domain;The protein component that mediates secretion through the outer membrane is contained within the secreted protein itself, hence the proteins secreted in this way are called autotransporters. This family corresponds to the presumed integral membrane beta-barrel domain that transports the protein. This domain is found at the C terminus of the proteins it occurs in. The N terminus contains the variable passenger domain that is translocated across the membrane. Once the passenger domain is exported it is cleaved auto-catalytically in some proteins, in others a different protease is used and in some cases no cleavage occurs.; autotransporter 1815325 4610006 azo1653 Azoarcus sp. BH72 autotransporter YP_933157.1 1812617 D 62928 CDS YP_933158.1 119897945 4606956 complement(1815335..1815595) 1 NC_008702.1 Homology to cell division topological specificity factor minE.; Family membership; putative cell division topological specificity factor 1815595 4606956 azo1654 Azoarcus sp. BH72 putative cell division topological specificity factor YP_933158.1 1815335 R 62928 CDS YP_933159.1 119897946 4606957 complement(1815597..1816409) 1 NC_008702.1 Septum site-determining protein minD (Cell division inhibitor minD).; High confidence in function and specificity; putative septum site-determining protein 1816409 minD 4606957 minD Azoarcus sp. BH72 putative septum site-determining protein YP_933159.1 1815597 R 62928 CDS YP_933160.1 119897947 4609224 complement(1816439..1817209) 1 NC_008702.1 Probable septum site-determining protein minC.; Family membership; putative cell division inhibitor 1817209 4609224 azo1656 Azoarcus sp. BH72 putative cell division inhibitor YP_933160.1 1816439 R 62928 CDS YP_933161.1 119897948 4606958 complement(1817301..1818167) 1 NC_008702.1 Putative 33 kDa chaperonin. Homology to hslO of E. coli of 32% (sprot|HSLO_ECOLI) Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress (By similarity). InterPro: Hsp33 protein (IPR000397) Pfam: Hsp33 protein no signal peptide no TMHs; Family membership; putative 33 kDa chaperonin 1818167 hslO 4606958 hslO Azoarcus sp. BH72 putative 33 kDa chaperonin YP_933161.1 1817301 R 62928 CDS YP_933162.1 119897949 4609034 complement(1818164..1818496) 1 NC_008702.1 45% Arsen_reductase.IPR006504; Cons_hypoth_ArsC. Pfam:PF03960; ArsC; 1. ArsC:arsenate reductase, catalyzes the reduction of arsenate to arsenite. Probably involved in arsenic resistance.; High confidence in function and specificity; putative arsenate reductase 1818496 4609034 azo1658 Azoarcus sp. BH72 putative arsenate reductase YP_933162.1 1818164 R 62928 CDS YP_933163.1 119897950 4606959 complement(1818545..1820866) 1 NC_008702.1 Transcription accessory protein (S1-domain containing RNA binding protein),; High confidence in function and specificity; transcription accessory protein 1820866 tex 4606959 tex Azoarcus sp. BH72 transcription accessory protein YP_933163.1 1818545 R 62928 CDS YP_933164.1 119897951 4609930 complement(1820983..1821834) 1 NC_008702.1 Possible aldose 1-epimerase.Aldose 1-epimerase (mutarotase), responsible for the anomeric interconversion of D-glucose and other aldoses between their alpha- and beta-forms. InterPro:IPR008183; Ald1_epimerase. Pfam:PF01263; Aldose_epim; 1.; hypothetical protein 1821834 4609930 azo1660 Azoarcus sp. BH72 hypothetical protein YP_933164.1 1820983 R 62928 CDS YP_933165.1 119897952 4606960 complement(1821839..1824526) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv3676 of C. violaceum of 47% (trembl|Q7NRV4). InterPro: DUF184 (IPR003781). Pfam: CoA binding domain; Acetyltransferase (GNAT) family. No signal peptide. No TMHs; hypothetical protein 1824526 4606960 azo1661 Azoarcus sp. BH72 hypothetical protein YP_933165.1 1821839 R 62928 CDS YP_933166.1 119897953 4606961 complement(1824697..1826376) 1 NC_008702.1 Putative two-component system sensor protein, 3 Signal P reporting signal peptide. TMHMM reporting 1 transmembrane helices.; Function unclear; putative two-component system sensor protein 1826376 4606961 azo1662 Azoarcus sp. BH72 putative two-component system sensor protein YP_933166.1 1824697 R 62928 CDS YP_933167.1 119897954 4606962 1826675..1827214 1 NC_008702.1 TREMBL:Q8EI75-35% identity, 54% similarity TREMBLnew:AAS81097 InterPro: SAM (and some other nucleotide) binding motif tehB: tellurite resistance protein TehB. Toxin production and resistance responsible for potassium tellurite resistance when present in high copy number, probably by increasing the reduction rate of tellurite to metallic tellurium within the bacterium. otherwise, phenotypically silent.cytoplasmic (potential). was originally (ref.1) thought to be plasmid encoded.InterPro IPR000051; SAM_bind. IPR004537; TehB. Pfam PF03848; TehB; 1. TIGRFAMs:TIGR00477; tehB; 1. INDUCES AGGLUTINATION OF NEURAMINIDASE-TREATED ERYTHROCYTES.; Conserved hypothetical protein; putative tellurite resistance protein 1827214 tehB 4606962 tehB Azoarcus sp. BH72 putative tellurite resistance protein YP_933167.1 1826675 D 62928 CDS YP_933168.1 119897955 4609926 complement(1827218..1828321) 1 NC_008702.1 catalyzes the selenophosphate-dependent transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA; tRNA 2-selenouridine synthase 1828321 4609926 azo1664 Azoarcus sp. BH72 tRNA 2-selenouridine synthase YP_933168.1 1827218 R 62928 CDS YP_933169.1 119897956 4606963 1828320..1829360 1 NC_008702.1 catalyzes the formation of selenophosphate from selenide and ATP; selenophosphate synthetase 1829360 4606963 azo1665 Azoarcus sp. BH72 selenophosphate synthetase YP_933169.1 1828320 D 62928 CDS YP_933170.1 119897957 4606964 complement(1829378..1829989) 1 NC_008702.1 Probable acyl-CoA thioesterase. Homology to tesA of E. coli of 42% (sprot|TESA_ECOLI) Hydrolyzes long chain acyl thioesters (c12-c18): 2-lysophosphatidylcholine + h(2)o = glycerophosphocholine + a fatty acid anion. InterPro: GDSL lipolytic enzyme (IPR001087) Pfam: Lipase/Acylhydrolase with GDSL-like motif signal peptide no TMH; Family membership; acyl-CoA thioesterase 1829989 tesA1 4606964 tesA1 Azoarcus sp. BH72 acyl-CoA thioesterase YP_933170.1 1829378 R 62928 CDS YP_933171.1 119897958 4609928 1829973..1830689 1 NC_008702.1 Similar to TREMBL:Q82UM8 (55% identity); TREMBL:Q8XYP0 (59% identity); TREMBL:Q7W685 (59% identity). Pfam (PF00005): ABC transporter.; Specificity unclear; putative ABC transporter ATP-binding protein 1830689 4609928 azo1667 Azoarcus sp. BH72 putative ABC transporter ATP-binding protein YP_933171.1 1829973 D 62928 CDS YP_933172.1 119897959 4606965 complement(1830700..1831530) 1 NC_008702.1 Phosphoserine phosphatase, SerB, catalyzes the final step in serine biosynthesis in E. coli. It hydrolyses the phosphate group from phosphoserine to yield serine. Similar to trembl|Q82X56 (54%) and to sprot|SERB_ECOLI (37%). TIGRFAM: phosphoserine phosphatase SerB Pfam (PF00702): haloacid dehalogenase-like hydrolase; Specificity unclear; phosphoserine phosphatase 1831530 serB 4606965 serB Azoarcus sp. BH72 phosphoserine phosphatase YP_933172.1 1830700 R 62928 CDS YP_933173.1 119897960 4609866 complement(1831527..1832603) 1 NC_008702.1 catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, tyrosine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase 1832603 aroF 4609866 aroF Azoarcus sp. BH72 phospho-2-dehydro-3-deoxyheptonate aldolase YP_933173.1 1831527 R 62928 CDS YP_933174.1 119897961 4608346 1832786..1833313 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA2869 of P.aeruginosa of 32% (trembl:Q9HZX7). No domains predicted. Signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 1833313 4608346 azo1670 Azoarcus sp. BH72 hypothetical protein YP_933174.1 1832786 D 62928 CDS YP_933175.1 119897962 4606966 1833366..1835738 1 NC_008702.1 GGDEF/EAL/PAS/PAC-domain containing protein; diguanylate cyclase 1835738 4606966 azo1671 Azoarcus sp. BH72 diguanylate cyclase YP_933175.1 1833366 D 62928 CDS YP_933176.1 119897963 4606967 complement(1835746..1836711) 1 NC_008702.1 Magnesium and cobalt transport protein CorA. Plays a role in the transport of Magnesium and Cobalt ions. Homolog to E.coli and S.typhimurium, magnesium and cobalt proteins. InterPro:IPR002523; Mg2+_transptCorA. Pfam: PF01544; CorA; 1. THM:2; Function unclear; putative magnesium and cobalt transport protein 1836711 corA 4606967 corA Azoarcus sp. BH72 putative magnesium and cobalt transport protein YP_933176.1 1835746 R 62928 CDS YP_933177.1 119897964 4608518 complement(1836734..1840186) 1 NC_008702.1 Transcription-repair coupling factor (TRCF). NECESSARY FOR STRAND-SPECIFIC REPAIR. A LESION IN THE TEMPLATE STRAND BLOCKS THE RNA POLYMERASE COMPLEX (RNAP). THE RNAP-DNA-RNA COMPLEX IS SPECIFICALLY RECOGNIZED BY TRCF WHICH RELEASES RNAP AND THE TRUNCATED TRANSCRIPT; THE TCRF MAY REPLACE RNAP AT THE LESION SITE AND THEN RECRUIT THE UVRA/B/C REPAIR SYSTEM. mfd: transcription-repair coupling fa; High confidence in function and specificity; transcription-repair coupling factor 1840186 mfd 4608518 mfd Azoarcus sp. BH72 transcription-repair coupling factor YP_933177.1 1836734 R 62928 CDS YP_933178.1 119897965 4609215 complement(1840295..1840948) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV1155 of Chromobacterium violaceum of 35% (gnl|keqq|cvi:CV1155(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1840948 4609215 azo1674 Azoarcus sp. BH72 hypothetical protein YP_933178.1 1840295 R 62928 CDS YP_933179.1 119897966 4606968 complement(1841025..1841771) 1 NC_008702.1 Probable DNA fragmentation factor 40 kDa subunit (EC 3.-.-.-) (DFF-40) (Caspase-activated deoxyribonuclease) (Caspase-activated DNase) (CAD). Members of PRT family are catalytic and regulatory proteins involved in nucleotide synthesis and salvage. The name PRT comes from phosphoribosyltransferase enzymes,which carry out phosphoryl transfer reactions on PRPP, an activated form of ribose-5-phosphate. This family includes a range of diverse phosphoribosyl transferase enzymes TREMBL:Q7WXA5: 29% identity, 47% similarity InterPro:IPR000836; PRtransferase. Pfam: PF00156; Pribosyltran; 1. acid_CoA_mut_C: methylmalonyl-CoA muta Non-secretory protein with low probability of signal peptide (0.001) No transmembrane helices; Function unclear; putative phosphoribosyltransferase 1841771 4606968 azo1675 Azoarcus sp. BH72 putative phosphoribosyltransferase YP_933179.1 1841025 R 62928 CDS YP_933180.1 119897967 4606969 complement(1841802..1842875) 1 NC_008702.1 conserved hypothetical pyruvat formate-lyase activiating enzyme. Homology to pflA of M. tuberculosis of 64% (trembl|P95188). no domains predicted no signal peptide no TMHs; Family membership; putative pyruvate formate-lyase activating enzyme 1842875 pflA 4606969 pflA Azoarcus sp. BH72 putative pyruvate formate-lyase activating enzyme YP_933180.1 1841802 R 62928 CDS YP_933181.1 119897968 4609529 complement(1842957..1843337) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03002649 of Dechloromonas aromatica of 39% (gi|41723559|ref|ZP_00150469.1|(NBCI ENTREZ)). No domains predicted. No TMH. No signal peptide.; hypothetical protein 1843337 4609529 azo1677 Azoarcus sp. BH72 hypothetical protein YP_933181.1 1842957 R 62928 CDS YP_933182.1 119897969 4606970 complement(1843392..1844072) 1 NC_008702.1 Putative cyclic nucleotide-binding protein,; Specificity unclear; putative cyclic nucleotide-binding protein 1844072 4606970 azo1678 Azoarcus sp. BH72 putative cyclic nucleotide-binding protein YP_933182.1 1843392 R 62928 CDS YP_933183.1 119897970 4606971 complement(1844113..1844679) 1 NC_008702.1 Hypothetical protein, 38% identity to SwissProt;O26945 Has PF01871;AMMECR1;(IPR002733, DUF 51)This family consists of several AMMECR1 as well as several uncharacterised proteins. The contiguous gene deletion syndrome AMME is characterised by Alport syndrome, midface hypoplasia, mental retardation and elliptocytosis and is caused by a deletion in Xq22.3,comprising several genes including COL4A5, FACL4 and AMMECR1. This family contains sequences from several eukaryotic species as well as archaebacteria and it has been suggested that the AMMECR1 protein may have a basic cellular function, potentially in either the transcription, replication, repair or translation machinery.; Specificity unclear; hypothetical protein 1844679 4606971 azo1679 Azoarcus sp. BH72 hypothetical protein YP_933183.1 1844113 R 62928 CDS YP_933184.1 119897971 4606972 complement(1844660..1845484) 1 NC_008702.1 Hypothetical UPF0103 protein ST2062. This gene is conserved in several species ranging from Caenorhabditis elegans and yeast to plants and micro-organisms although no function has yet been ascribed to the other proteins in this family. TREMBL:Q843N3: 38% identity,52% similarity InterPro:IPR002733; DUF51. IPR002737; DUF52. Pfam: PF01871; AMMECR1; 1. PF01875; UPF0103 Signal peptide present No transmembrane helices holB: DNA polymerase III delta prime sub; Function unclear; hypothetical protein 1845484 4606972 azo1680 Azoarcus sp. BH72 hypothetical protein YP_933184.1 1844660 R 62928 CDS YP_933185.1 119897972 4606973 complement(1845532..1846800) 1 NC_008702.1 Conserved hypothetical membrane protein. TrEMBL; Q82Y76(48% identity). TMHMM2 reporting 14 TMH's present. Signal Peptide not present. Has PF02308;MgtC family;(IPR003416 MgtCSapB_transpt)The MgtC protein is found in an operon with the Mg2+ transporter protein MgtB. The function of MgtC and its homologues is not known, but it is thought that MgtC may act as an accessory protein for MgtB, thus mediating magnesium influx into the cytosol. Also included in this family are the Bacillus subtilis SapB protein and several hypothetical proteins. Has PF04093 rod shape-determining protein MreD;IPR007227; MreD (murein formation D) is involved in the rod shape determination in E. coli, and more generally in cell shape determination of bacteria whether or not they are rod-shaped.; Conserved hypothetical protein; hypothetical protein 1846800 4606973 azo1681 Azoarcus sp. BH72 hypothetical protein YP_933185.1 1845532 R 62928 CDS YP_933186.1 119897973 4606974 1846917..1847627 1 NC_008702.1 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; forms homodimers; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1847627 ispD 4606974 ispD Azoarcus sp. BH72 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase YP_933186.1 1846917 D 62928 CDS YP_933187.1 119897974 4606975 1847624..1848112 1 NC_008702.1 catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 1848112 ispF 4606975 ispF Azoarcus sp. BH72 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase YP_933187.1 1847624 D 62928 CDS YP_933188.1 119897975 4609097 complement(1848221..1848658) 1 NC_008702.1 Conserved hypothetical protein. Homology to pc0921 of Parachlamydia_UWE25 of 34% (pcu:pc0921(KEGG)). No signal peptide reported to be present. No TMH reported to be present. No domains predicted.; hypothetical protein 1848658 4609097 azo1684 Azoarcus sp. BH72 hypothetical protein YP_933188.1 1848221 R 62928 CDS YP_933189.1 119897976 4606976 complement(1848851..1849654) 1 NC_008702.1 Conserved hypothetical protein. Homology to NE0619 of Nitrosomonas europaea of 44% (trembl:Q82WP5). Has PF06167, IPR010384; Protein of unknown function (DUF980);Family of uncharacterised bacterial sequences. No signal peptide. No TMHs.; hypothetical protein 1849654 4606976 azo1685 Azoarcus sp. BH72 hypothetical protein YP_933189.1 1848851 R 62928 CDS YP_933190.1 119897977 4606977 complement(1849657..1850562) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV2531 of C. violaceum of 42% (tremble:Q7NV15) InterPro: Integral membrane protein DUF6 Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transport Pfam: Integral membrane protein DUF6 no signal peptide probable 10 TMHS; Conserved hypothetical protein; hypothetical protein 1850562 4606977 azo1686 Azoarcus sp. BH72 hypothetical protein YP_933190.1 1849657 R 62928 CDS YP_933191.1 119897978 4606978 complement(1850648..1853737) 1 NC_008702.1 Similar to membrane carboxypeptidases.; Family membership; hypothetical protein 1853737 4606978 azo1687 Azoarcus sp. BH72 hypothetical protein YP_933191.1 1850648 R 62928 CDS YP_933192.1 119897979 4606979 1853847..1856048 1 NC_008702.1 GTP pyrophosphokinase,; High confidence in function and specificity; GTP diphosphokinase 1856048 relA 4606979 relA Azoarcus sp. BH72 GTP diphosphokinase YP_933192.1 1853847 D 62928 CDS YP_933193.1 119897980 4609712 complement(1856085..1858166) 1 NC_008702.1 catalyzes the reversible transfer of the terminal phosphate of ATP to form a long chain polyphosphate; polyphosphate kinase 1858166 ppk 4609712 ppk Azoarcus sp. BH72 polyphosphate kinase YP_933193.1 1856085 R 62928 CDS YP_933194.1 119897981 4609612 1858259..1859809 1 NC_008702.1 45% Ppx_GppA. Pfam:PF02541; Ppx-GppA; 1.; High confidence in function and specificity; putative exopolyphosphatase 1859809 ppx 4609612 ppx Azoarcus sp. BH72 putative exopolyphosphatase YP_933194.1 1858259 D 62928 CDS YP_933195.1 119897982 4609617 1859915..1861012 1 NC_008702.1 Conserved hypothetical membrane protein. Similar to TREMBL:Q8Y2M2 (43% identity). InterPro (IPR003453): Domain of unknown function DUF140. Pfam (PF02405): Domain of unknown function DUF140. TIGRFAM (TIGR00056): Conserved hypothetical protein. TMHMM reporting five transmembrane helices.; hypothetical protein 1861012 4609617 azo1691 Azoarcus sp. BH72 hypothetical protein YP_933195.1 1859915 D 62928 CDS YP_933196.1 119897983 4606980 1861009..1861785 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q7W0L5 (51% identity); TREMBL:Q9A5X8 (46% identity). InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003593): AAA ATPase. InterPro (IPR003439): ABC transporter. Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; putative ABC transporter ATP-binding protein 1861785 4606980 azo1692 Azoarcus sp. BH72 putative ABC transporter ATP-binding protein YP_933196.1 1861009 D 62928 CDS YP_933197.1 119897984 4606981 1861775..1862713 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bb0165 of B. bronchiseptica of 31% (TREMBL:Q7WR07). Pfam: mce related protein This family of proteins contains the mce (mycobacterial cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonisation of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria. Signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 1862713 4606981 azo1693 Azoarcus sp. BH72 hypothetical protein YP_933197.1 1861775 D 62928 CDS YP_933198.1 119897985 4606982 1862703..1863353 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA6519 of Azoarcus sp. EbN1 of 44% (gnl|keqq|eba:ebA6519(KEGG)). no domains predicted. signal peptide present. no TMHs.; hypothetical protein 1863353 4606982 azo1694 Azoarcus sp. BH72 hypothetical protein YP_933198.1 1862703 D 62928 CDS YP_933199.1 119897986 4606983 1863402..1865054 1 NC_008702.1 activates fatty acids by binding to coenzyme A; acyl-CoA synthetase 1865054 4606983 azo1695 Azoarcus sp. BH72 acyl-CoA synthetase YP_933199.1 1863402 D 62928 CDS YP_933200.1 119897987 4606984 1865171..1865971 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 1865971 paaF2 4606984 paaF2 Azoarcus sp. BH72 enoyl-CoA hydratase YP_933200.1 1865171 D 62928 CDS YP_933201.1 119897988 4609471 1865974..1866795 1 NC_008702.1 Putative deoxyribonuclease yjjV (EC 3.1.21.-). InterPro: Uncharacterized protein family UPF0006. TIGR00010: deoxyribonuclease TatD family.; High confidence in function and specificity; putative deoxyribonuclease 1866795 yjjV 4609471 yjjV Azoarcus sp. BH72 putative deoxyribonuclease YP_933201.1 1865974 D 62928 CDS YP_933202.1 119897989 4610191 complement(1866763..1867386) 1 NC_008702.1 Tetracycline repressor protein class G. Similar to SWISSPROT: sprot|TER7_VIBAN (21% Vibrio anguillarum (Listonella anguillarum), tetracycline repressor protein class G). TETR IS THE REPRESSOR OF THE TETRACYCLINE RESISTANCE ELEMENT; ITS AMINO-TERMINAL REGION FORMS A HELIX-TURN-HELIX STRUCTURE AND BINDS DNA. BINDING OF TETRACYCLINE TO TETR REDUCES THE REPRESSOR AFFINITY FOR THE TETRACYCLINE RESISTANCE GENE (TETA) PROMOTER OPERATOR SITES. InterPro: IPR001647 HTH_TetR. Pfam: PF00440 Bacterial regulatory proteins, tetR family. HTH reporting nucleic acid binding motif.; Family membership; TetR family transcriptional regulator 1867386 4610191 azo1698 Azoarcus sp. BH72 TetR family transcriptional regulator YP_933202.1 1866763 R 62928 CDS YP_933203.1 119897990 4606985 1867564..1868313 1 NC_008702.1 Electron transfer flavoprotein, beta subunit. Homology to etfB of B. japonicum of 71% (sprot|ETFB_BRAJA). The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). Pfam: Electron transfer flavoprotein beta subunit no signal peptide no TMHS; High confidence in function and specificity; electron transfer flavoprotein subunit beta 1868313 etfB1 4606985 etfB1 Azoarcus sp. BH72 electron transfer flavoprotein subunit beta YP_933203.1 1867564 D 62928 CDS YP_933204.1 119897991 4608682 1868313..1869248 1 NC_008702.1 Probable electron transfer flavoprotein, alpha subunit. Homology to etfA of B. japonicum of 65% (sprot|ETFA_BRAJA) The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein alpha-subunit (IPR001308) Pfam: Electron transfer flavoprotein alpha subunit no signal peptide no TMHs; High confidence in function and specificity; electron transfer flavoprotein subunit alpha 1869248 etfA1 4608682 etfA1 Azoarcus sp. BH72 electron transfer flavoprotein subunit alpha YP_933204.1 1868313 D 62928 CDS YP_933205.1 119897992 4608678 1869332..1869952 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV1589 of Chromobacterium violaceum of 36% (trembl|Q7NXN7(SRS)). Has PF01891;CbiM;(IPR002751)This integral membrane protein is involved in cobalamin synthesis. No signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 1869952 4608678 azo1701 Azoarcus sp. BH72 hypothetical protein YP_933205.1 1869332 D 62928 CDS YP_933206.1 119897993 4606986 1870013..1871812 1 NC_008702.1 Acyl-CoA dehydrogenase short/branched chain specific mitochondrial precursor (2-methyl branched chain acyl-CoA dehydrogenase) (2-MEBCAD) (2-methylbutyryl-coenzyme A dehydrogenase) (2-methylbutyryl-CoA dehydrogenase). Has greatest activity toward short branched chain acyl- CoA derivative such as (s)-2-methylbutyryl-CoA isobutyryl-CoA and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl- CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent. Entry name TREMBL:Q8Y0W8 Identities = 358/596 (60%) InterPro IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1.; Family membership; acyl-CoA dehydrogenase 1871812 4606986 azo1702 Azoarcus sp. BH72 acyl-CoA dehydrogenase YP_933206.1 1870013 D 62928 CDS YP_933207.1 119897994 4606987 complement(1871903..1872685) 1 NC_008702.1 Cell binding factor 2 precursor (Major antigen peb4A). Homology to cbf2 of C. jejuni of 34% (sprot|CBF2_CAMJE). Belongs to the ppic/parvulin rotamase family InterPro: PpiC-type peptidyl-prolyl cis-trans isomerase (IPR000297) Pfam: PPIC-type PPIASE domain signal peptide no TMH; Family membership; putative cell binding factor 1872685 cbf2 4606987 cbf2 Azoarcus sp. BH72 putative cell binding factor YP_933207.1 1871903 R 62928 CDS YP_933208.1 119897995 4608423 complement(1872682..1872975) 1 NC_008702.1 Region start changed from 1873054 to 1872976 (-78 bases); putative BolA-like protein 1872975 4608423 azo1704 Azoarcus sp. BH72 putative BolA-like protein YP_933208.1 1872682 R 62928 CDS YP_933209.1 119897996 4606988 complement(1872972..1873271) 1 NC_008702.1 unknown function; YciI from Haemophilus influenzae presents crystal structure similarity to a muconolactone isomerase, but does not seem to catalyze any of the predicted reactions based on sequence and structure similarity; YciI-like protein 1873271 yciI 4606988 yciI Azoarcus sp. BH72 YciI-like protein YP_933209.1 1872972 R 62928 CDS YP_933210.1 119897997 4610161 complement(1873271..1873879) 1 NC_008702.1 Involved in cell division; probably involved in intracellular septation; intracellular septation protein A 1873879 ispZ 4610161 ispZ Azoarcus sp. BH72 intracellular septation protein A YP_933210.1 1873271 R 62928 CDS YP_933211.1 119897998 4609098 complement(1873923..1874336) 1 NC_008702.1 Conserved hypothetical globin-like protein. Homology to bd2638 of B. bacteriovorus of 57% (tremblnew|CAE80431). Globins are heme-containing proteins involved in binding and/or transporting oxygen. This family of heme binding proteins are found mainly in bacteria. However they can also be found in some protozoa and plants as well. InterPro: Protozoan/cyanobacterial globins (IPR001486) Pfam: Bacterial-like globin. no signal peptide. no TMHs; Family membership; globin-like protein 1874336 4609098 azo1707 Azoarcus sp. BH72 globin-like protein YP_933211.1 1873923 R 62928 CDS YP_933212.1 119897999 4606989 complement(1874441..1875826) 1 NC_008702.1 Probable osmolarity sensor protein EnvZ,; Specificity unclear; osmolarity sensor protein 1875826 envZ1 4606989 envZ1 Azoarcus sp. BH72 osmolarity sensor protein YP_933212.1 1874441 R 62928 CDS YP_933213.1 119898000 4608671 complement(1875852..1876598) 1 NC_008702.1 part of two-component system EnvZ/OmpR; regulates transcription of outer membrane porin genes ompC/F; under high osmolarity EnvZ functions as kinase/phosphotransferase and phosphorylates OmpR; the result is increased expression of ompC and repression of ompF; also functions in regulation of other genes; forms dimers upon phosphorylation; osmolarity response regulator 1876598 ompR 4608671 ompR Azoarcus sp. BH72 osmolarity response regulator YP_933213.1 1875852 R 62928 CDS YP_933214.1 119898001 4609446 1876813..1877142 1 NC_008702.1 Hypothetical secreted protein. No homologs in the database. Signal Peptide present. No TMHs. No domains predicted; hypothetical protein 1877142 4609446 azo1710 Azoarcus sp. BH72 hypothetical protein YP_933214.1 1876813 D 62928 CDS YP_933215.1 119898002 4606990 1877212..1877901 1 NC_008702.1 Conserved hypothetical glycoprotease. Homology to yeaZ of N. europae of 41% (trembl|Q82US9). InterPro: Glycoprotease (M22) metallo-protease family (IPR000905). Tigrfam: gcp: metalloendopeptidase putative glycoprotease family. Pfam: Glyocprotease family. no signal peptide. no TMHs.; Family membership; glycoprotease 1877901 4606990 azo1711 Azoarcus sp. BH72 glycoprotease YP_933215.1 1877212 D 62928 CDS YP_933216.1 119898003 4606991 1877931..1878353 1 NC_008702.1 Conserved hypothetical ribosomal-protein-alanine acetyltransferase. Homology to ebA6491 of Azoarcus sp. EbN1 of 63% (gnl|keqq|eba:ebA6491(KEGG)). Pfam: Acetyltransferase (GNAT) family. Tigrfam: ribosomal-protein-alanine acetyltransferase. No signal peptide. No TMHs.; Conserved hypothetical protein; putative ribosomal-protein-alanine acetyltransferase 1878353 4606991 azo1712 Azoarcus sp. BH72 putative ribosomal-protein-alanine acetyltransferase YP_933216.1 1877931 D 62928 CDS YP_933217.1 119898004 4606992 1878350..1879201 1 NC_008702.1 Putative DNA polymerase-related protein,bacteriophage-type. TIGRFAM: rad23: UV excision repair protein Rad23; Specificity unclear; DNA-directed DNA polymerase 1879201 4606992 azo1713 Azoarcus sp. BH72 DNA-directed DNA polymerase YP_933217.1 1878350 D 62928 CDS YP_933218.1 119898005 4606993 complement(1879223..1880473) 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. Homolog to the hypothetical protein ygeD, a putative resistence protein from Y. pestis(by similarity). 2A0121: H+ Antiporter protein; High confidence in function and specificity; lysophospholipid transporter LplT 1880473 4606993 azo1714 Azoarcus sp. BH72 lysophospholipid transporter LplT YP_933218.1 1879223 R 62928 CDS YP_933219.1 119898006 4606994 1880629..1881549 1 NC_008702.1 Trans-acting transcriptional regulator of RuBisCO genes expression Putative HTH-type transcriptional regulator Contains 1 HTH lysR-type DNA-binding domain,belonds to the LysR family InterPro: Bacterial regulatory protein LysR family ModE_repress: ModE molybdate transport 32% HTH_LysR. IPR005119; LysR_subst. IPR009058; Wing_hlx_DNA_bnd. PF00126; HTH_1; 1. PF03466; LysR_substrate; 1; High confidence in function and specificity; RuBisCO operon transcriptional regulator (RbcR) 1881549 rbcR 4606994 rbcR Azoarcus sp. BH72 RuBisCO operon transcriptional regulator (RbcR) YP_933219.1 1880629 D 62928 CDS YP_933220.1 119898007 4609695 1881549..1882919 1 NC_008702.1 Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents; DNA repair protein RadA 1882919 radA 4609695 radA Azoarcus sp. BH72 DNA repair protein RadA YP_933220.1 1881549 D 62928 CDS YP_933221.1 119898008 4609690 complement(1882984..1883250) 1 NC_008702.1 Hypothetical protein . Extremely weak homology with any hits in the database. No Features,signal Peptide,motifs are known domains present.; hypothetical protein 1883250 4609690 azo1717 Azoarcus sp. BH72 hypothetical protein YP_933221.1 1882984 R 62928 CDS YP_933222.1 119898009 4606995 1883575..1884198 1 NC_008702.1 Hypothetical protein, has very weak homology with hits in the Database(TrEMBL;Q63I29) Coils2 program reporting presence of coiled-coiled.; hypothetical protein 1884198 4606995 azo1718 Azoarcus sp. BH72 hypothetical protein YP_933222.1 1883575 D 62928 CDS YP_933223.1 119898010 4606996 1884227..1885696 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA7025 of Azoarcus sp. EbN1 of 68% (gnl|keqq|eba:ebA7025(KEGG)). Pfam: Glycos_transf_2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids. No signal peptide. No TMHs; hypothetical protein 1885696 4606996 azo1719 Azoarcus sp. BH72 hypothetical protein YP_933223.1 1884227 D 62928 CDS YP_933224.1 119898011 4606997 1885699..1886832 1 NC_008702.1 Family membership; glycosyltransferase 1886832 4606997 azo1720 Azoarcus sp. BH72 glycosyltransferase YP_933224.1 1885699 D 62928 CDS YP_933225.1 119898012 4606998 complement(1886756..1887814) 1 NC_008702.1 Similar to waaQ.; Family membership; heptosyltransferase 1887814 4606998 azo1721 Azoarcus sp. BH72 heptosyltransferase YP_933225.1 1886756 R 62928 CDS YP_933226.1 119898013 4606999 1887936..1888283 1 NC_008702.1 Hypothetical protein, weak homology with hits. TrEMBL;Q9KPT2(45% identity) No domains, repeats, motifs or features present.; hypothetical protein 1888283 4606999 azo1722 Azoarcus sp. BH72 hypothetical protein YP_933226.1 1887936 D 62928 CDS YP_933227.1 119898014 4607000 1888362..1889357 1 NC_008702.1 Conserved hypothetical protein. Homology to blr5713 of B.japonicum of 45% (tremble:Q89IC6). No domains predicted. No TMHs. SignalP 3.0 predicts no signal peptide.; hypothetical protein 1889357 4607000 azo1723 Azoarcus sp. BH72 hypothetical protein YP_933227.1 1888362 D 62928 CDS YP_933228.1 119898015 4607001 1889404..1889835 1 NC_008702.1 Conserved hypothetical protein. Homology to SMA1058 of S.meliloti of 48% (trembl:Q92ZB7). No domains predicted. No signal peptide. No TMHs; hypothetical protein 1889835 4607001 azo1724 Azoarcus sp. BH72 hypothetical protein YP_933228.1 1889404 D 62928 CDS YP_933229.1 119898016 4607002 1889835..1891748 1 NC_008702.1 catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching protein 1891748 glgB1 4607002 glgB1 Azoarcus sp. BH72 glycogen branching protein YP_933229.1 1889835 D 62928 CDS YP_933230.1 119898017 4608885 1891732..1893780 1 NC_008702.1 O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety.Probable glucanohydrolase Pep1B. Could convert glycogen into units that may be subsequently converted into trehalose.Belongs to family 13 of glycosyl hydrolases. Glge subfamily. 47% Alpha_amyl_cat. Pfam:PF00128; alpha-amylase; 1.; High confidence in function and specificity; putative glucanohydrolase 1893780 pep1 4608885 pep1 Azoarcus sp. BH72 putative glucanohydrolase YP_933230.1 1891732 D 62928 CDS YP_933231.1 119898018 4609518 1893796..1897116 1 NC_008702.1 Trehalose synthase(Maltose alpha-D-glucosyltransferase).Catalyzes the conversion of maltose into alpha,alpha- trehalose by transglucosylation. 54% Alp_amyl_cat_sub.IPR006047; Alpha_amyl_cat.InterPro: Glycoside hydrolase family 13. Pfam:PF00128; alpha-amylase; 1.; High confidence in function and specificity; putative trehalose synthase 1897116 treS 4609518 treS Azoarcus sp. BH72 putative trehalose synthase YP_933231.1 1893796 D 62928 CDS YP_933232.1 119898019 4609966 complement(1897139..1897705) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BPP3320 of B.bronchiseptica of 31% (tremble:Q7W5H1). No domains predicted. Signap P reporting signal peptide. No TMH reported.; Conserved hypothetical protein; hypothetical protein 1897705 4609966 azo1728 Azoarcus sp. BH72 hypothetical protein YP_933232.1 1897139 R 62928 CDS YP_933233.1 119898020 4607003 1897982..1899142 1 NC_008702.1 Conserved hypothetical protein. Homology to mm2747 of M. mazei of 39% (trembl|Q8PTG8(SRS). no domains predicted. no TMHs. no signal peptide.; hypothetical protein 1899142 4607003 azo1729 Azoarcus sp. BH72 hypothetical protein YP_933233.1 1897982 D 62928 CDS YP_933234.1 119898021 4607004 1899198..1899947 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA6994 of Azoarcus sp. EbN1 of 59% (gnl|keqq|eba:ebA6994(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1899947 4607004 azo1730 Azoarcus sp. BH72 hypothetical protein YP_933234.1 1899198 D 62928 CDS YP_933235.1 119898022 4607005 1899978..1900238 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domain prediction. No signal peptide. 1 TMH; hypothetical protein 1900238 4607005 azo1731 Azoarcus sp. BH72 hypothetical protein YP_933235.1 1899978 D 62928 CDS YP_933236.1 119898023 4607006 1900291..1901157 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,; Family membership; short-chain dehydrogenase 1901157 4607006 azo1732 Azoarcus sp. BH72 short-chain dehydrogenase YP_933236.1 1900291 D 62928 CDS YP_933237.1 119898024 4607007 complement(1901147..1901629) 1 NC_008702.1 Hypothetical protein with unknown function. Domain predicted by Pfam, PF01381 Helix-turn-helix.; hypothetical protein 1901629 4607007 azo1733 Azoarcus sp. BH72 hypothetical protein YP_933237.1 1901147 R 62928 CDS YP_933238.1 119898025 4607008 1901759..1904050 1 NC_008702.1 Entry name TREMBL:Q89PM4 Identities = 257/546 (47%) Prediction: Non-secretory protein Signal peptide probability: 0.018 Number of predicted TMHs: 0 pts-lac: PTS system lactose/cellobios; hypothetical protein 1904050 4607008 azo1734 Azoarcus sp. BH72 hypothetical protein YP_933238.1 1901759 D 62928 CDS YP_933239.1 119898026 4607009 1904135..1904476 1 NC_008702.1 Conserved hypothetical protein. Homology to rO1139 of S. meliloti of 48% (trembl|Q92QZ6). No domains predicted. No signal peptide. No TMHs; hypothetical protein 1904476 4607009 azo1735 Azoarcus sp. BH72 hypothetical protein YP_933239.1 1904135 D 62928 CDS YP_933240.1 119898027 4607010 complement(1904512..1905063) 1 NC_008702.1 Hypothetical protein. No good homology with any Hits in the database.; hypothetical protein 1905063 4607010 azo1736 Azoarcus sp. BH72 hypothetical protein YP_933240.1 1904512 R 62928 CDS YP_933241.1 119898028 4607011 1905343..1906143 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA6987 of Azoarcus sp. EbN1 of 62% (gnl|keqq|eba:ebA6987(KEGG)). no domains predicted. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 1906143 4607011 azo1737 Azoarcus sp. BH72 hypothetical protein YP_933241.1 1905343 D 62928 CDS YP_933242.1 119898029 4607012 1906182..1906676 1 NC_008702.1 Hypothetical protein has weak homology to Transglycosylase of penicillin-binding protein 1c(pbpC).TrEMBL:Q8ZN47(56%). ***Contains PRC-barrel domain(pfam05239). The PRC-barrel is an all beta barrel domain found in photosystem reaction centre subunit H of the purple bacteria and RNA metabolism proteins of the RimM group. PRC-barrels are approximately 80 residues long, and found widely represented in bacteria, archaea and plants. This domain is also present at the carboxyl terminus of the pan-bacterial protein RimM, which is involved in ribosomal maturation and processing of 16S rRNA. A family of small proteins conserved in all known euryarchaea are composed entirely of a single stand-alone copy of the domain. No Signal Peptide reported. No TMH's reported.; hypothetical protein 1906676 4607012 azo1738 Azoarcus sp. BH72 hypothetical protein YP_933242.1 1906182 D 62928 CDS YP_933243.1 119898030 4607013 complement(1906742..1907686) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:O32291 (45% identity); SWISSPROT:P54554 (28% identity). Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short chain dehydrogenase 1907686 4607013 azo1739 Azoarcus sp. BH72 short chain dehydrogenase YP_933243.1 1906742 R 62928 CDS YP_933244.1 119898031 4607014 1907834..1908745 1 NC_008702.1 conserved hypothetical protein, 49% identity to TrEMBL;Q7W766. Has SMART;SM00559;Ku78;Ku70 and Ku80 are 70kDa and 80kDa subunits of the Lupus Ku autoantigen;(IPR006164);This is a single stranded DNA- and ATP-depedent helicase that has a role in chromosome translocation. This is a domain of unknown function C-terminal to its von Willebrand factor A domain, that also occurs in bacterial hypothetical proteins.; hypothetical protein 1908745 4607014 azo1740 Azoarcus sp. BH72 hypothetical protein YP_933244.1 1907834 D 62928 CDS YP_933245.1 119898032 4607015 1908782..1911427 1 NC_008702.1 catalyzes the ATP dependent formation of a phosphodiester at the site of a single-strand break in duplex DNA; ATP-dependent DNA ligase 1911427 ligD 4607015 ligD Azoarcus sp. BH72 ATP-dependent DNA ligase YP_933245.1 1908782 D 62928 CDS YP_933246.1 119898033 4607016 1911701..1912390 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. Signal peptide present. No TMHs.; hypothetical protein 1912390 4607016 azo1742 Azoarcus sp. BH72 hypothetical protein YP_933246.1 1911701 D 62928 CDS YP_933247.1 119898034 4607017 complement(1912374..1913198) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA6983 of Azoarcus sp. EbN1 of 50% (gnl|keqq|eba:ebA6983(KEGG)). Pfam: N-formylglutamate amidohydrolase. Formylglutamate amidohydrolase (FGase) catalyses the terminal reaction in the five-step pathway for histidine utilisation in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolysed to produce L-glutamate plus formate. no signal peptide. no TMHs; hypothetical protein 1913198 4607017 azo1743 Azoarcus sp. BH72 hypothetical protein YP_933247.1 1912374 R 62928 CDS YP_933248.1 119898035 4607018 complement(1913153..1914424) 1 NC_008702.1 Hypothetical protein,32% identity (49% similarity) to SwissProt;O27977 Has PF04107;Glutamate-cysteine ligase family 2(GCS2);(IPR006336)Also known as gamma-glutamylcysteine synthetase and gamma-ECS. This enzyme catalyses the first and rate limiting step in de novo glutathione biosynthesis. Members of this family are found in archaea, bacteria and plants. May and Leaver [1] discuss the possible evolutionary origins of glutamate-cysteine ligase enzymes in different organisms and suggest that it evolved independently in different eukaryotes,from an ancestral bacterial enzyme. They also state that Arabidopsis thaliana gamma-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast and Escherichia coli homologues. In plants, there are separate cytosolic and chloroplast forms of the enzyme.; glutamate-cysteine ligase 1914424 4607018 azo1744 Azoarcus sp. BH72 glutamate-cysteine ligase YP_933248.1 1913153 R 62928 CDS YP_933249.1 119898036 4607019 complement(1914421..1915890) 1 NC_008702.1 Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase) rimK_fam: S6 modification enzyme RimK; Specificity unclear; ribosomal protein S6 modification protein-like protein 1915890 rimK 4607019 rimK Azoarcus sp. BH72 ribosomal protein S6 modification protein-like protein YP_933249.1 1914421 R 62928 CDS YP_933250.1 119898037 4609727 complement(1915934..1916671) 1 NC_008702.1 Conserved hypothetical protein. Homology to TdenA01000334 of Thiobacillus denitrificans of 67% (gi|52008038|ref|ZP_00335415.1|(NBCI ENTREZ)). No domains present. No signal peptide. No TMHs.; hypothetical protein 1916671 4609727 azo1746 Azoarcus sp. BH72 hypothetical protein YP_933250.1 1915934 R 62928 CDS YP_933251.1 119898038 4607020 complement(1916675..1917442) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No TMHs Signal Peptide present. Has PS50914; BON;The BON (bacterial OsmY and nodulation) domain is found in the bacterial osmotic-shock-resistance protein OsmY, a family of haemolysins, a group of nodulation specificity proteins and secretory channels, and several hypothetical proteins. It is typically about 60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions.; hypothetical protein 1917442 4607020 azo1747 Azoarcus sp. BH72 hypothetical protein YP_933251.1 1916675 R 62928 CDS YP_933252.1 119898039 4607021 1917567..1918007 1 NC_008702.1 Hypothetical protein yhcV. TREMBL:Q92V69:54%identity, 76% similarity. TREMBL:Q81UY6 Pfam:CBS domain, Anticodon binding domain. TIGRFAM: KpsF/GutQ family proteins. No signal peptide or transmembrane helix reported. CBS domains are small intracellular modules of unknown function. They are mostly found in 2 or four copies within a protein. Pairs of CBS domains dimerise to form a stable globular domain. Two CBS domains are found in inosine-monophosphate dehydrogenase from all species, however the CBS domains are not needed for activity. CBS domains are found attached to a wide range of other protein domains suggesting that CBS domains may play a regulatory role. The region containing the CBS domains in Cystathionine-beta synthase is involved in regulation by S-AdoMet.. A number of disease states are associated with CBS-containing proteins including homocystinuria, Becker's and Thomsen disease. InterPro: CBS domain urease_gam: urease gamma subunit; Function unclear; hypothetical protein 1918007 4607021 azo1748 Azoarcus sp. BH72 hypothetical protein YP_933252.1 1917567 D 62928 CDS YP_933253.1 119898040 4607022 complement(1918070..1918228) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to r02018 of S. meliloti of 72% (trembl|Q92K67(SRS). no damains predicted .signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 1918228 4607022 azo1749 Azoarcus sp. BH72 hypothetical protein YP_933253.1 1918070 R 62928 CDS YP_933254.1 119898041 4607023 1918212..1918685 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein to the data bank. Has DUF1328(Protein of Unknown family) domain. PF07043;IPR009760; This family consists of several hypothetical bacterial proteins of around 50 residues in length. The function of this family is unknown. no signal peptide. 3 TMHs; hypothetical protein 1918685 4607023 azo1750 Azoarcus sp. BH72 hypothetical protein YP_933254.1 1918212 D 62928 CDS YP_933255.1 119898042 4607024 1918699..1919052 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA6968 of Azoarcus sp. EbN1 of 38% (gnl|keqq|eba:ebA6968(KEGG)). Pfam: Putative phospholipid-binding domain This domain is found in a family of osmotic shock protection proteins (e.g. P27291). It is also found in some Secretins and a group of potential haemolysins. Its likely function is attachment to phospholipid membranes. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 1919052 4607024 azo1751 Azoarcus sp. BH72 hypothetical protein YP_933255.1 1918699 D 62928 CDS YP_933256.1 119898043 4607025 1919143..1919466 1 NC_008702.1 Conserved hypothetical secreted protein: Homology to ne0129 of N. europaea of 43% (trembl|Q82XW6). Smart: Bacterial OsmY and nodulation domain (BON). The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 1919466 4607025 azo1752 Azoarcus sp. BH72 hypothetical protein YP_933256.1 1919143 D 62928 CDS YP_933257.1 119898044 4607026 1919438..1920847 1 NC_008702.1 Function:- Cardiolipin synthetase (CL synthase),cls. Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Entry name :-SWISSPROT:CLS_ECOLI Prim. accession # P31071 InterPro:IPR001736; PLD. Identities = 114/379 (30%) Pfam:PF00614; PLDc; 2. TMhelix=1 Signal peptide probability: 1.0; Family membership; putative cardiolipin synthetase 1920847 cls2 4607026 cls2 Azoarcus sp. BH72 putative cardiolipin synthetase YP_933257.1 1919438 D 62928 CDS YP_933258.1 119898045 4608493 1920896..1921315 1 NC_008702.1 Conserved hypothetical membrane protein. Homology Avin02003871 of Azotobacter vinelandii of 47% (gi|53610588|ref|ZP_00089122.2|(NBCI ENTREZ)). Has PF03653, Uncharacterised protein family (UPF0093). Interpro: IPR005265; Cons_hypoth701; It appears the conserved hypothetical integral membrane proteins of this family are found only in gram negative bacteria and their function is unknown. Signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 1921315 4608493 azo1754 Azoarcus sp. BH72 hypothetical protein YP_933258.1 1920896 D 62928 CDS YP_933259.1 119898046 4607027 complement(1921312..1921959) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology ebA159 of Azoarcus sp. EbN1 of 52% (gnl|keqq|eba:ebA159(KEGG)). No domains predicted. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 1921959 4607027 azo1755 Azoarcus sp. BH72 hypothetical protein YP_933259.1 1921312 R 62928 CDS YP_933260.1 119898047 4607028 1922011..1922682 1 NC_008702.1 Probable quniol oxidase, subunit II. Homology to qoxB of Rhodobacter sphaeroides of 41% (gi|4416093|gb|AAD20226.1|(NBCI ENTREZ)). InterPro: Cytochrome c oxidase subunit II (IPR002429); Cu(A) centre of cytochrome c oxidase, subunit II and nitrous oxide reductase (IPR001505). Pfam: Cytochrome c oxidase subunit II, periplasmic. no signal peptide. 2 TMHs; Family membership; quniol oxidase subunit II 1922682 qoxB 4607028 qoxB Azoarcus sp. BH72 quniol oxidase subunit II YP_933260.1 1922011 D 62928 CDS YP_933261.1 119898048 4609685 1922679..1925261 1 NC_008702.1 Probable quinol oxidase, subunit I. Homology to qoxA of R. sphaeroides of 52% (trembl|Q9Z605). InterPro: Cytochrome c oxidase subunit III (IPR000298); Cytochrome coxidase, subunit I (IPR000883) Pfam: Cytochrom C and quinol oxidase poly polypeptide signal peptide 17 TMHs; Family membership; quinol oxidase subunit I 1925261 qoxA 4609685 qoxA Azoarcus sp. BH72 quinol oxidase subunit I YP_933261.1 1922679 D 62928 CDS YP_933262.1 119898049 4609684 1925258..1925599 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted. no signal peptide. 3 TMHs; hypothetical protein 1925599 4609684 azo1758 Azoarcus sp. BH72 hypothetical protein YP_933262.1 1925258 D 62928 CDS YP_933263.1 119898050 4607029 1925590..1926420 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Avin02003353 of Azotobacter vinelandii of 48% (gi|53610930|ref|ZP_00342175.1|(NBCI ENTREZ)). No domains predicted. signal peptide. 7 TMHs; Conserved hypothetical protein; hypothetical protein 1926420 4607029 azo1759 Azoarcus sp. BH72 hypothetical protein YP_933263.1 1925590 D 62928 CDS YP_933264.1 119898051 4607030 1926417..1927421 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8KUH7 (41% identity); SWISSPROT:P14802 (30% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short chain dehydrogenase 1927421 4607030 azo1760 Azoarcus sp. BH72 short chain dehydrogenase YP_933264.1 1926417 D 62928 CDS YP_933265.1 119898052 4607031 complement(1927438..1931043) 1 NC_008702.1 Hypothetical protein, Shares 45% similarity with hypothetical protein [Chromobacterium violaceum]TrEMBL Q7NY28. Signal Peptide present. No TMH's reported. Has pfam05359, DUF748, Domain of Unknown Function (DUF748).; Specificity unclear; hypothetical protein 1931043 4607031 azo1761 Azoarcus sp. BH72 hypothetical protein YP_933265.1 1927438 R 62928 CDS YP_933266.1 119898053 4607032 complement(1931040..1932170) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to XCC0079 of Xanthomonas campestris (trembl|Q8PEA2(SRS)). InterPro:IPR002549; UPF0118. Pfam PF01594; domain of DUF20 present. no signal peptide. 8 TMHs; Conserved hypothetical protein; hypothetical protein 1932170 4607032 azo1762 Azoarcus sp. BH72 hypothetical protein YP_933266.1 1931040 R 62928 CDS YP_933267.1 119898054 4607033 complement(1932167..1933585) 1 NC_008702.1 Probable serine protease MucD. Homology to mucD of P. aeruginosa of 40% (trembl|Q57155). InterPro: Serine proteases trypsin family (IPR001254); Chymotrypsin serine protease family (S1) (IPR001314); HtrA/DegQ protease family (IPR001940); PDZ domain (also known as DHR or GLGF) (IPR001478) Pfam: Trypsin; PDZ domain (also known as DHR or GLGF) signal peptide no TMHs TIGR00292: thiazole biosynthesis enzyme; High confidence in function and specificity; serine protease MucD 1933585 mucD3 4607033 mucD3 Azoarcus sp. BH72 serine protease MucD YP_933267.1 1932167 R 62928 CDS YP_933268.1 119898055 4609285 1933759..1934097 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bb3588 of B. bronchiseptica of 41% (trembl|Q7WGK1). Smart: Bacterial OsmY and nodulation domain (BON). The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins. signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 1934097 4609285 azo1764 Azoarcus sp. BH72 hypothetical protein YP_933268.1 1933759 D 62928 CDS YP_933269.1 119898056 4607034 1934286..1934459 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Atu4467 of Agrobacterium tumefaciens of 58% (trembl|Q8U7I2). Has PF07043, Protein of unknown function (DUF1328);IPR009760;This family consists of several hypothetical bacterial proteins of around 50 residues in length. The function of this family is unknown. signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 1934459 4607034 azo1765 Azoarcus sp. BH72 hypothetical protein YP_933269.1 1934286 D 62928 CDS YP_933270.1 119898057 4607035 complement(1934490..1935986) 1 NC_008702.1 Probable NADH dehydrogenase. Homology to ndh of P. fluorescens of 48% (trembl|Q9KGX0). TRANSFER OF ELECTRONS FROM NADH TO THE RESPIRATORY CHAIN. THE IMMEDIATE ELECTRON ACCEPTOR FOR THE ENZYME IS BELIEVED TO BE UBIQUINONE. DOES NOT COUPLE THE REDOX REACTION TO PROTON TRANSLOCATION. Interpro: FAD-dependent pyridine nucleotide-dislphide oxidoreductase (IPR001327) Pfam: Pyridine nucleotide-disluphide oxidoreductase no signal peptide no TMHs; Family membership; NADH dehydrogenase 1935986 ndh1 4607035 ndh1 Azoarcus sp. BH72 NADH dehydrogenase YP_933270.1 1934490 R 62928 CDS YP_933271.1 119898058 4609348 complement(1936009..1936383) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Polyangium cellulosum of 69% (tremble:Q9L8D1). Has PF07100;(IPR009794)Protein of unknown function (DUF1362);This family consists of several hypothetical bacterial proteins of around 125 resides in length. The function of this family is unknown. No signal peptide or TMH reported present.; hypothetical protein 1936383 4609348 azo1767 Azoarcus sp. BH72 hypothetical protein YP_933271.1 1936009 R 62928 CDS YP_933272.1 119898059 4607036 1936817..1937137 1 NC_008702.1 Hypothetical membrane protein. no homology of the entire protein to the data bank. no domains predicted no signal peptide 1 TMH; hypothetical protein 1937137 4607036 azo1768 Azoarcus sp. BH72 hypothetical protein YP_933272.1 1936817 D 62928 CDS YP_933273.1 119898060 4607037 complement(1937286..1938023) 1 NC_008702.1 Conserved hypothetical protein. Weak homology with hits in the database. Coils2 program predicts presence of coiled coil.; hypothetical protein 1938023 4607037 azo1769 Azoarcus sp. BH72 hypothetical protein YP_933273.1 1937286 R 62928 CDS YP_933274.1 119898061 4607038 1938484..1938891 1 NC_008702.1 Conserved hypothetical DnaK suppressor protein. Homology to BPSS2280 of Burkholderia pseudomallei of 34% (gnl|keqq|bps:BPSS2280(KEGG). Has PF01258, Prokaryotic dksA/traR C4-type zinc finger; IPR000962 Znf_DskA/TraR; The dksA gene product suppresses the temperature-sensitive growth and filamentation of a dnaK deletion mutant of Escherichia coli. Gene knockout and deletion experiments have shown the gene to be non-essential, mutations causing a mild sensitivity to UV light, but not affecting DNA recombination. The protein contains a C-terminal region thought to fold into a 4-cysteine zinc finger. Other proteins found to contain a similar domain include the traR gene product, present on the conjugative plasmid F,and several hypothetical proteins from bacteria and bacteriophages. No TMHs. No signal peptide.; Conserved hypothetical protein; DnaK suppressor protein 1938891 4607038 azo1770 Azoarcus sp. BH72 DnaK suppressor protein YP_933274.1 1938484 D 62928 CDS YP_933275.1 119898062 4607039 1938905..1940323 1 NC_008702.1 Probable cyanide insensitive terminal oxidase,subunit I Homology to cioA of P. aeruginose of 44% (TREMBL:O07440) InterPro: Cytochrome bd ubiquinol oxidase subunit I (IPR002585) Pfam: Bacterial cytochrome ubiquinol oxidase no signal peptide no TMHs; High confidence in function and specificity; cyanide insensitive terminal oxidase subunit I 1940323 cioA 4607039 cioA Azoarcus sp. BH72 cyanide insensitive terminal oxidase subunit I YP_933275.1 1938905 D 62928 CDS YP_933276.1 119898063 4608483 1940316..1941338 1 NC_008702.1 Probable cyanide insensitive terminal oxidase,subunit II. Homology to cioB of P. aeruginosa of 40% (trembl|O07441). Tigrfam: cydB: cytochrome d ubiquinol oxidase, subunit II Pfam: cytochrome oxidase, subunit II no signal peptide 8 TMHs; High confidence in function and specificity; cyanide insensitive terminal oxidase subunit II 1941338 cioB 4608483 cioB Azoarcus sp. BH72 cyanide insensitive terminal oxidase subunit II YP_933276.1 1940316 D 62928 CDS YP_933277.1 119898064 4608484 1941818..1942150 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of P. putida of 58% (trembl|O68643). no TMHs. no signal peptide. No domains predicted.; hypothetical protein 1942150 4608484 azo1773 Azoarcus sp. BH72 hypothetical protein YP_933277.1 1941818 D 62928 CDS YP_933278.1 119898065 4607040 1942244..1943044 1 NC_008702.1 Conserved hypothetical protein. Homology to Avin02003958 of Azotobacter vinelandii of 38% (gi|53610498|ref|ZP_00089565.2|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1943044 4607040 azo1774 Azoarcus sp. BH72 hypothetical protein YP_933278.1 1942244 D 62928 CDS YP_933279.1 119898066 4607041 complement(1943128..1943709) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 1943709 4607041 azo1775 Azoarcus sp. BH72 hypothetical protein YP_933279.1 1943128 R 62928 CDS YP_933280.1 119898067 4607042 1943822..1944037 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 1944037 4607042 azo1776 Azoarcus sp. BH72 hypothetical protein YP_933280.1 1943822 D 62928 CDS YP_933281.1 119898068 4607043 1944084..1945133 1 NC_008702.1 Hypothetical LuxR-family transcriptional regulator. Similarity to other known proteins (dctR or narP or esaR) only in the domain part. Pfam: PF00196 Bacterial regulatory proteins, luxR family. HTH reporting nucleic acid binding motif. The 'helix-turn-helix' DNA-binding motif of this protein is located in the C-terminal section of the sequence.; Conserved hypothetical protein; LuxR family transcriptional regulator 1945133 4607043 azo1777 Azoarcus sp. BH72 LuxR family transcriptional regulator YP_933281.1 1944084 D 62928 CDS YP_933282.1 119898069 4607044 1945382..1945816 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 3 TMHs; hypothetical protein 1945816 4607044 azo1778 Azoarcus sp. BH72 hypothetical protein YP_933282.1 1945382 D 62928 CDS YP_933283.1 119898070 4607045 1945848..1946708 1 NC_008702.1 This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls (preferentially: D-lactyl-L-Ala). InterPro (IPR002502): N-acetylmuramoyl-L-alanine amidase (family 2). Pfam (PF01510): N-acetylmuramoyl-L-alanine amidase.; Family membership; N-acetylmuramoyl-L-alanine amidase 1946708 ampD1 4607045 ampD1 Azoarcus sp. BH72 N-acetylmuramoyl-L-alanine amidase YP_933283.1 1945848 D 62928 CDS YP_933284.1 119898071 4608312 1946826..1947452 1 NC_008702.1 Hypothetical protein. no homology of the entire protein to the data bank InterPro: Putative peptidoglycan binding domain 1 (IPR002477) Pfam: Putative peptidoglycan binding domain 1 no signal peptide no TMHs; hypothetical protein 1947452 4608312 azo1780 Azoarcus sp. BH72 hypothetical protein YP_933284.1 1946826 D 62928 CDS YP_933285.1 119898072 4607046 complement(1948908..1949294) 1 NC_008702.1 Conserved hypothetical protein. Homology to map2952a of M. paratuberculosis of 51% (AAS05269). Pfam: CahB; Rho terminator factor, N-terminal. domain no signal peptide. no TMHs; hypothetical protein 1949294 4607046 azo1781 Azoarcus sp. BH72 hypothetical protein YP_933285.1 1948908 R 62928 CDS YP_933286.1 119898073 4607047 complement(1949308..1950150) 1 NC_008702.1 Conserved hypothetical protein. Homology to mlr2853 of M. loti of 31% (trembl|Q98HI7). Tigrfam: xth: exodeoxyribonuclease III (xth). All proteins in this family for which functions are known are 5' AP endonucleases that function in base excision repair and the repair of abasic sites in DNA. Pfam: AP endonuclease family I. no signal peptide. no TMHs; hypothetical protein 1950150 4607047 azo1782 Azoarcus sp. BH72 hypothetical protein YP_933286.1 1949308 R 62928 CDS YP_933287.1 119898074 4607048 complement(1950122..1952401) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA6946 of Azoarcus sp. EbN1 of 64% (gnl|keqq|eba:ebA6946(KEGG)). Pfam: Pospholipase D, active site motife; DedA family. no signal peptide. 6 TMHs; Conserved hypothetical protein; hypothetical protein 1952401 4607048 azo1783 Azoarcus sp. BH72 hypothetical protein YP_933287.1 1950122 R 62928 CDS YP_933288.1 119898075 4607049 complement(1952414..1952968) 1 NC_008702.1 Bacterioferritin (BFR) (Cytochrome B-1) (Cytochrome B-557). In E. coli is an iron-storage protein consisting of 24 identical subunits that pack together to form a highly symmetrical, nearly spherical shell surrounding a central cavity of about 8 nm diameter.X-ray crystallographic studies have revealed a close structural similarity between BFR and the ferritins, a family of iron-storage proteins found in both eukaryota and prokaryota. Key role in iron homeostasis.InterPro: Bacterioferritin. Non-secretory protein bfr: bacterioferritin; High confidence in function and specificity; putative bacterioferritin 1952968 bfr3 4607049 bfr3 Azoarcus sp. BH72 putative bacterioferritin YP_933288.1 1952414 R 62928 CDS YP_933289.1 119898076 4608388 1953247..1954419 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Family membership; glycosyltransferase 1954419 4608388 azo1785 Azoarcus sp. BH72 glycosyltransferase YP_933289.1 1953247 D 62928 CDS YP_933290.1 119898077 4607050 1954400..1954948 1 NC_008702.1 Conserved hypothetical phosphoesterase family protein. Homology to ebA6940 of Azoarcus sp. EbN1 of 65% (gnl|keqq|eba:ebA6940(KEGG)). InterPro IPR008934; AcPase_VanPerase. IPR000326; PA_PTPase. Pfam PF01569; PAP2; Number of predicted TMHs: 3#. Prediction: Non-secretory protein Signal peptide probability: 0.000; Conserved hypothetical protein; phosphoesterase family protein 1954948 4607050 azo1786 Azoarcus sp. BH72 phosphoesterase family protein YP_933290.1 1954400 D 62928 CDS YP_933291.1 119898078 4607051 complement(1954864..1955775) 1 NC_008702.1 TIGR00093: conserved hypothetical protein; Function unclear; hypothetical protein 1955775 4607051 azo1787 Azoarcus sp. BH72 hypothetical protein YP_933291.1 1954864 R 62928 CDS YP_933292.1 119898079 4607052 complement(1955772..1956905) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Family membership; glycosyltransferase 1956905 4607052 azo1788 Azoarcus sp. BH72 glycosyltransferase YP_933292.1 1955772 R 62928 CDS YP_933293.1 119898080 4607053 complement(1957010..1957564) 1 NC_008702.1 Conserved hypothetical protein. Homology to bp0770 of B. pertussis of 37% (TrEMBL:Q7VZW1). no domains reported. no signal peptide. no TMHs; hypothetical protein 1957564 4607053 azo1789 Azoarcus sp. BH72 hypothetical protein YP_933293.1 1957010 R 62928 CDS YP_933294.1 119898081 4607054 complement(1957602..1959068) 1 NC_008702.1 RNA polymerase sigma-54 factor. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Similar to SWISSPROT: sprot|RP54_ALCEU (46% Alcaligenes eutrophus (Ralstonia eutropha), RpoN or NtrA) / sprot|RP54_ECOLI (41% Escherichia coli, RpoN or NtrA) Pfam:PF00309 Sigma54_AID. PF04963 Sigma54_CBD. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; RNA polymerase sigma-54 factor 1959068 rpoN2 4607054 rpoN2 Azoarcus sp. BH72 RNA polymerase sigma-54 factor YP_933294.1 1957602 R 62928 CDS YP_933295.1 119898082 4609801 1959413..1960084 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA6930 of Azoarcus sp. EbN1 of 37% (gnl|keqq|eba:ebA6930(KEGG)). No domains predicted. Signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 1960084 4609801 azo1791 Azoarcus sp. BH72 hypothetical protein YP_933295.1 1959413 D 62928 CDS YP_933296.1 119898083 4607055 1960085..1961215 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to xcc0923 of X. campestris of 46% (trembl|Q8PC30). InterPro: Uncharacterized protein family UPF0003 (IPR006685). Pfam: Mechanosensitive ion chanell. Mechanosensitive (MS) channels provide protection against hypo-osmotic shock, responding both to stretching of the cell membrane and to membrane depolarisation. no signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 1961215 4607055 azo1792 Azoarcus sp. BH72 hypothetical protein YP_933296.1 1960085 D 62928 CDS YP_933297.1 119898084 4607056 1961212..1962507 1 NC_008702.1 Conserved hypothetical protein.Weak Homology with Hits in the Database. Has IPR000967 Znf_NFX1;(SMART;SM00438)This domain is presumed to be a zinc binding domain. The following pattern describes the zinc finger: C-X(1-6)-H-X-C-X3-C(H/C)-X(3-4)-(H/C)-X(1-10)-C, where X can be any amino acid, and numbers in brackets indicate the number of residues. The two position can be either his or cys. This domain is found in the human transcriptional repressor NK-X1, a repressor of HLA-DRA transcription; the Drosophila shuttle craft protein, which plays an essential role during the late stages of embryonic neurogenesis; and a yeast hypothetical protein YNL023C. No signal peptide or TMH reported present.; hypothetical protein 1962507 4607056 azo1793 Azoarcus sp. BH72 hypothetical protein YP_933297.1 1961212 D 62928 CDS YP_933298.1 119898085 4607057 1962504..1964684 1 NC_008702.1 Glycogen operon protein GlgX homolog.Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen. 46% Alpha_amyl_cat.IPR004193; Glyco_hydro_13N. Pfam:PF00128; alpha-amylase; 1.F02922; isoamylase_N; 1.; High confidence in function and specificity; putative glycogen debranching enzyme 1964684 glgX 4607057 glgX Azoarcus sp. BH72 putative glycogen debranching enzyme YP_933298.1 1962504 D 62928 CDS YP_933299.1 119898086 4608888 1964688..1965785 1 NC_008702.1 DNA topoisomerase I (EC 5.99.1.2). The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. cbiB: cobalamin biosynthesis protein.; Specificity unclear; hypothetical protein 1965785 4608888 azo1795 Azoarcus sp. BH72 hypothetical protein YP_933299.1 1964688 D 62928 CDS YP_933300.1 119898087 4607058 1965782..1967599 1 NC_008702.1 catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching protein 1967599 glgB2 4607058 glgB2 Azoarcus sp. BH72 glycogen branching protein YP_933300.1 1965782 D 62928 CDS YP_933301.1 119898088 4608886 1967706..1968920 1 NC_008702.1 catalyzes the formation of ADP-glucose and diphosphate from ATP and alpha-D-glucose 1-phosphate; glucose-1-phosphate adenylyltransferase 1968920 glgC 4608886 glgC Azoarcus sp. BH72 glucose-1-phosphate adenylyltransferase YP_933301.1 1967706 D 62928 CDS YP_933302.1 119898089 4608887 1968917..1970977 1 NC_008702.1 Malto-oligosyltrehalose trehalohydrolase(MTHase) (4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase) (Maltooligosyl trehalose trehalohydrolase).Involved in the trehalose biosynthesis,hydrolysing alpha-(1->4)-d-glucosidic linkage in 4-alpha-d-{(1->4)-alpha-d-glucanosyl}(n) trehalose to yield trehalose and alpha-(1->4)-d-glucan. 42% Alpha_amyl_cat.IPR004193; Glyco_hydro_13N. Pfam:PF00128; alpha-amylase; 1.PF02922; isoamylase_N; 1.; Specificity unclear; putative malto-oligosyltrehalose trehalohydrolase 1970977 treZ 4608887 treZ Azoarcus sp. BH72 putative malto-oligosyltrehalose trehalohydrolase YP_933302.1 1968917 D 62928 CDS YP_933303.1 119898090 4609967 1971003..1976207 1 NC_008702.1 amylomaltase; acts to release glucose from maltodextrins; putative bifunctional 4-alpha-glucanotransferase/malto-oligosyltrehalose synthase 1976207 4609967 azo1799 Azoarcus sp. BH72 putative bifunctional 4-alpha-glucanotransferase/malto-oligosyltrehalose synthase YP_933303.1 1971003 D 62928 CDS YP_933304.1 119898091 4607059 complement(1976211..1977182) 1 NC_008702.1 Glucokinase (EC 2.7.1.2) (Glucose kinase). Catalytic activity: ATP + d-glucose = ADP+ d-glucose 6-phosphate. 34% Glucokinase. Pfam:PF02685; Glucokinase; 1. TIGRFAMs:TIGR00749; glk; 1.; High confidence in function and specificity; putative glucokinase 1977182 glk1 4607059 glk1 Azoarcus sp. BH72 putative glucokinase YP_933304.1 1976211 R 62928 CDS YP_933305.1 119898092 4608889 1977251..1978927 1 NC_008702.1 Glycogen synthase (Starch [bacterial glycogen] synthase) GlgA. Synthesizes alpha-14-glucan chains using ADP-glucose. 46% Glyco_trans_1. Pfam:PF00534; Glycos_transf_1; 1.; High confidence in function and specificity; putative glycogen synthase 1978927 glgA 4608889 glgA Azoarcus sp. BH72 putative glycogen synthase YP_933305.1 1977251 D 62928 CDS YP_933306.1 119898093 4608884 complement(1978903..1979856) 1 NC_008702.1 67% Epimerase_Dh. Pfam: PF01370; Epimerase; 1. No signal peptide.; Family membership; putative dTDP-glucose 4,6-dehydratase 1979856 4608884 azo1802 Azoarcus sp. BH72 putative dTDP-glucose 4,6-dehydratase YP_933306.1 1978903 R 62928 CDS YP_933307.1 119898094 4607060 complement(1979858..1980823) 1 NC_008702.1 Conserved hypothetical glycosyltransferase. Homology to ebA6914 of Azoarcus sp. EbN1 of 72% (gnl|keqq|eba:ebA6914(KEGG)). Pfam: Glycos_transf_1. Interpro: Glycosyl transferase, group 1. Proteins containign this domain transfer UDP, ADP, GDP or CMP linked sugars to a variety of substrates, including glycogen, fructose-6-phosphate and lipopolysaccharides. The bacterial enzymes are involved in various biosynthetic processes that include exopolysaccharide biosynthesis,lipopolysaccharide core biosynthesis and the biosynthesis of the slime polysaccaride colanic acid. No signal peptide. No TMHs.; Conserved hypothetical protein; glycosyltransferase 1980823 4607060 azo1803 Azoarcus sp. BH72 glycosyltransferase YP_933307.1 1979858 R 62928 CDS YP_933308.1 119898095 4607061 complement(1980849..1981829) 1 NC_008702.1 Hypothetical 55.3 kDa protein in thcA 5region (ORF1).; High confidence in function and specificity; membrane-anchored glycosyltransferase 1981829 4607061 azo1804 Azoarcus sp. BH72 membrane-anchored glycosyltransferase YP_933308.1 1980849 R 62928 CDS YP_933309.1 119898096 4607062 complement(1981826..1983562) 1 NC_008702.1 Hypothetical conserved hypothetical cabamoyltransferase. Homology to sco6480 of S. coelicolor of 49% (trembl|Q8CJN9). Pfam: Carbamyltransferase. no signal peptide. no TMHS; Conserved hypothetical protein; carbamoyltransferase 1983562 4607062 azo1805 Azoarcus sp. BH72 carbamoyltransferase YP_933309.1 1981826 R 62928 CDS YP_933310.1 119898097 4607063 complement(1983567..1984781) 1 NC_008702.1 ADP-heptose--LPS heptosyltransferase II (EC 2.-.-.-). pdxJ: pyridoxal phosphate biosynthetic; High confidence in function and specificity; ADP-heptose--LPS heptosyltransferase II 1984781 waaF1 4607063 waaF1 Azoarcus sp. BH72 ADP-heptose--LPS heptosyltransferase II YP_933310.1 1983567 R 62928 CDS YP_933311.1 119898098 4610105 complement(1984769..1985425) 1 NC_008702.1 Hypothetical protein yaeD.; High confidence in function and specificity; heptose phosphatase 1985425 4610105 azo1807 Azoarcus sp. BH72 heptose phosphatase YP_933311.1 1984769 R 62928 CDS YP_933312.1 119898099 4607064 complement(1985416..1986156) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8YLL2 (35% identity); TREMBL:Q9HWN3 (37% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 1986156 4607064 azo1808 Azoarcus sp. BH72 short-chain dehydrogenase YP_933312.1 1985416 R 62928 CDS YP_933313.1 119898100 4607065 complement(1986153..1987412) 1 NC_008702.1 Hypothetical protein Rv0486/MT0504/Mb0496. InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 1987412 4607065 azo1809 Azoarcus sp. BH72 glycosyltransferase YP_933313.1 1986153 R 62928 CDS YP_933314.1 119898101 4607066 1987667..1988008 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 1988008 4607066 azo1810 Azoarcus sp. BH72 hypothetical protein YP_933314.1 1987667 D 62928 CDS YP_933315.1 119898102 4607067 complement(1987987..1988523) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BPP2658 of B. parapertussis of 48% (trembl|Q7W772(SRS)). 3 TMHs. no signal protein. Has PF04239;Protein of unknown function (DUF421);IPR007353 DUF421; Conserved hypothetical protein; hypothetical protein 1988523 4607067 azo1811 Azoarcus sp. BH72 hypothetical protein YP_933315.1 1987987 R 62928 CDS YP_933316.1 119898103 4607068 1988694..1990121 1 NC_008702.1 Probable transcriptional regulatory protein,; Specificity unclear; transcriptional regulator 1990121 4607068 azo1812 Azoarcus sp. BH72 transcriptional regulator YP_933316.1 1988694 D 62928 CDS YP_933317.1 119898104 4607069 1990267..1990617 1 NC_008702.1 Hypothetical protein. Weak homology with hits.; hypothetical protein 1990617 4607069 azo1813 Azoarcus sp. BH72 hypothetical protein YP_933317.1 1990267 D 62928 CDS YP_933318.1 119898105 4607070 1990819..1991112 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD123 of Azoarcus sp. EbN1 of 50% (gnl|keqq|eba:ebD123(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 1991112 4607070 azo1814 Azoarcus sp. BH72 hypothetical protein YP_933318.1 1990819 D 62928 CDS YP_933319.1 119898106 4607071 1991120..1991503 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein with the data bank. No domains predicted. No signal peptide. 2 TMHs; hypothetical protein 1991503 4607071 azo1815 Azoarcus sp. BH72 hypothetical protein YP_933319.1 1991120 D 62928 CDS YP_933320.1 119898107 4607072 1991500..1991976 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted No signal peptide 2 TMHs; hypothetical protein 1991976 4607072 azo1816 Azoarcus sp. BH72 hypothetical protein YP_933320.1 1991500 D 62928 CDS YP_933321.1 119898108 4607073 1992008..1995052 1 NC_008702.1 Conserved hypothetical two-component hybrid sensor and regulator. Homology to ebA6890 of Azoarcus sp. EbN1 of 56% (gnl|keqq|eba:ebA6890(KEGG)). InterPro: IPR003594 ATPbind_ATPase. IPR004358 Bact_sens_pr_C. IPR005467 His_kinase. IPR003661 His_kinA_N. IPR009082 His_kin_homodim. IPR001610 PAC. IPR000700 PAS-assoc_C. IPR000014 PAS_domain. IPR001789 Response_reg. Pfam: PF02518 HATPase_c. PF00512 HisKA. PF00785 PAC. PF00989 PAS. PF00072 Response_reg. SMART: SM00387 HATPase_c. SM00388 HisKA. SM00086 PAC. SM00091 PAS. SM00448 REC. TIGRFAM: TIGR00229 sensory_box; 3 TMHMM reporting 7 transmembrane helices.; Conserved hypothetical protein; putative two-component hybrid sensor and regulator 1995052 4607073 azo1817 Azoarcus sp. BH72 putative two-component hybrid sensor and regulator YP_933321.1 1992008 D 62928 CDS YP_933322.1 119898109 4607074 1995107..1996027 1 NC_008702.1 Putative ribonuclease RNASE BN (RBN)Protein, 31% identity (44% similarity) to TrEMBL; Q8XPH6. Has PF03631; Ribonuclease BN-like family;IPR004664, RNase_BN;This family contains integral membrane proteins with 5 to 6 predicted transmembrane spans. The family include ribonuclease BN that is involved in tRNA maturation P32146. This family of proteins does not seem to contain any completely conserved polar residues that would be expected in a nuclease enzyme, suggesting that many members of this family may not have this catalytic activity.; Family membership; exoribonuclease II 1996027 4607074 azo1818 Azoarcus sp. BH72 exoribonuclease II YP_933322.1 1995107 D 62928 CDS YP_933323.1 119898110 4607075 1996190..1997581 1 NC_008702.1 Hypothetical protein. Homology to dr0392 of D. radiodurans of 25% (trembl|Q9RXC3) Smart: Bacterial OsmY and nodulation domain (BON). The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins. no signal peptide no TMHs; High confidence in function and specificity; hypothetical protein 1997581 4607075 azo1819 Azoarcus sp. BH72 hypothetical protein YP_933323.1 1996190 D 62928 CDS YP_933324.1 119898111 4607076 1997651..1998100 1 NC_008702.1 Conserved hypothetical protein. Homology to BB2852 of B.bronchiseptica of 53% (tremble:Q7WIJ9). Has pfam05239, PRC, PRC-barrel domain. The PRC-barrel is an all beta barrel domain found in photosystem reaction centre subunit H of the purple bacteria and RNA metabolism proteins of the RimM group. PRC-barrels are approximately 80 residues long, and found widely represented in bacteria, archaea and plants. This domain is also present at the carboxyl terminus of the pan-bacterial protein RimM, which is involved in ribosomal maturation and processing of 16S rRNA. A family of small proteins conserved in all known euryarchaea are composed entirely of a single stand-alone copy of the domain. NO signal peptide reported to be present. No TMH reported to be present.; hypothetical protein 1998100 4607076 azo1820 Azoarcus sp. BH72 hypothetical protein YP_933324.1 1997651 D 62928 CDS YP_933325.1 119898112 4607077 1998158..2000881 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA6882 of Azoarcus sp. EbN1 of 74% (gnl|keqq|eba:ebA6882(KEGG)). Domian structure: 373 aa -599 aa AAA ATPase; 638 aa - 726 aa HATPase_c; 750 aa - 877 aa Rec InterPro: ABC transporter (IPR003439); AAA ATPase superfamily (IPR003593); ABC transporter transmembrane region (IPR001140); ATP/GTP-binding site motif A ((P-loop) (IPR001687); (Response regulator reciver (IPR001789); Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase (IPR003594) Pfam: ABC-transporter ABC transmembrane region; Response regulator reciver domain. no signal peptide. probable 5 TMHs; Conserved hypothetical protein; hypothetical protein 2000881 4607077 azo1821 Azoarcus sp. BH72 hypothetical protein YP_933325.1 1998158 D 62928 CDS YP_933326.1 119898113 4607078 2000904..2001464 1 NC_008702.1 Conserved hypothetical protein. Signal peptide present.; hypothetical protein 2001464 4607078 azo1822 Azoarcus sp. BH72 hypothetical protein YP_933326.1 2000904 D 62928 CDS YP_933327.1 119898114 4607079 2001475..2002209 1 NC_008702.1 Putative Mg(2+) transporter ATPase [attV], 58% identity (77% similarity) to TrEMBL;Q8UKG4, Q9WWC7. TrEMBBL;Q7W456(66% identity). Has PF02308;MgtC family;(IPR003416, MgtCSapB_transpt):The MgtC protein is found in an operon with the Mg2+ transporter protein MgtB. The function of MgtC and its homologues is not known. No Signal peptide or TMH reported present.; Family membership; putative Mg(2+) transporter 2002209 4607079 azo1823 Azoarcus sp. BH72 putative Mg(2+) transporter YP_933327.1 2001475 D 62928 CDS YP_933328.1 119898115 4607080 complement(2002246..2002746) 1 NC_008702.1 Protein ygaD. TREMBL:Q7W761: 58% identity, 68% similarity CinA is a DNA damage- or competence-inducible protein that is polycistronic with recA in a number of species. Several bacterial species have a protein consisting largely of the C-terminal domain of CinA but lacking the N-terminal domain. InterPro: Competence-damaged protein InterPro: IPR008136; CinA_C. Pfam: PF02464 cinA_cterm: competence/damage-inducible No Signal peptide (Signal P predicted) No transmembrane helices; Function unclear; CinA domain-containing protein 2002746 ygaD2 4607080 ygaD2 Azoarcus sp. BH72 CinA domain-containing protein YP_933328.1 2002246 R 62928 CDS YP_933329.1 119898116 4610176 complement(2002713..2003273) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to smb20339 of S. meliloti of 30% (trembl|Q92WL4(SRS)). No domains predicted. signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2003273 4610176 azo1825 Azoarcus sp. BH72 hypothetical protein YP_933329.1 2002713 R 62928 CDS YP_933330.1 119898117 4607081 2003309..2004646 1 NC_008702.1 39%; NADH dehydrogenase 2004646 4607081 azo1826 Azoarcus sp. BH72 NADH dehydrogenase YP_933330.1 2003309 D 62928 CDS YP_933331.1 119898118 4607082 2004654..2005118 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CC2898 of Caulobacter crescentus of 56% (trembl|Q89WY3(SRS)). No domains predicted. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2005118 4607082 azo1827 Azoarcus sp. BH72 hypothetical protein YP_933331.1 2004654 D 62928 CDS YP_933332.1 119898119 4607083 complement(2005129..2006316) 1 NC_008702.1 Hypothetical secreted protein. No significant homology over the entire protein length with the data bank. InterPro: Oxidoreductase FAD and NAD(P)-binding domain (IPR001433); Ferredoxin (IPR001041); NADH: cytochrome b5 reductase (IPR001834). Pfam: Oxidoreductase FAD-binding domain; Oxidoreductase NAD-binding domain; 2Fe-2S iron-sulfur cluster binding domain. signal peptide. no TMHs; hypothetical protein 2006316 4607083 azo1828 Azoarcus sp. BH72 hypothetical protein YP_933332.1 2005129 R 62928 CDS YP_933333.1 119898120 4607084 complement(2006232..2007008) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. Signal peptide. 1 TMHs; hypothetical protein 2007008 4607084 azo1829 Azoarcus sp. BH72 hypothetical protein YP_933333.1 2006232 R 62928 CDS YP_933334.1 119898121 4607085 complement(2007034..2009067) 1 NC_008702.1 Similar to FepA, an iron regulated outer membrane protein. FepA is a receptor for the siderophore complex ferric enterobactin and for colcins B and D. INVOLVED IN THE INITIAL STEP OF IRON UPTAKE BY BINDING FERRIC ENTEROBACTIN THAT ALLOWS E. COLI TO EXTRACT IRON FROM THE ENVIRONMENT. ilvC: ketol-acid reductoisomerase; Function unclear; putative TonB-denpendent receptor 2009067 4607085 azo1830 Azoarcus sp. BH72 putative TonB-denpendent receptor YP_933334.1 2007034 R 62928 CDS YP_933335.1 119898122 4607086 2009546..2010928 1 NC_008702.1 Di-heme cytochrome c peroxidase MauG, required for the synthesis of the tryptophan tryptophylquinone (TTQ) prosthetic group of MADH in P.denitrificans . INVOLVED IN METHYLAMINE METABOLISM. ESSENTIAL FOR THE MATURATION OF THE BETA SUBUNIT OF MADH PRESUMABLY VIA A STEP IN THE BIOSYNTHESIS OF TRYPTOPHAN TRYPTOPHYLQUINONE (TTQ) THE COFACTOR OF MADH. urease_gam: urease gamma subunit; High confidence in function and specificity; putative cytochrome c551 peroxidase 2010928 mauG 4607086 mauG Azoarcus sp. BH72 putative cytochrome c551 peroxidase YP_933335.1 2009546 D 62928 CDS YP_933336.1 119898123 4609200 2010939..2011565 1 NC_008702.1 Conserved hypothetical secreted protein. Homology ebA6930 of Azoarcus EbN1 of 31% (gnl|keqq|eba:ebA6930(KEGG)). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 2011565 4609200 azo1832 Azoarcus sp. BH72 hypothetical protein YP_933336.1 2010939 D 62928 CDS YP_933337.1 119898124 4607087 2011690..2012379 1 NC_008702.1 DNA-3-methyladenine glycosylase 1 (3-methyladenine DNA glycosidase 1) (3MEA DNA glycosylase 1). HYDROLYSIS OF THE DEOXYRIBOSE N-GLYCOSIDIC BOND TO EXCISE 3-METHYLADENINE OR 7-METHYLADENINE FROM THE DAMAGED DNA POLYMER FORMED BY ALKYLATION LESIONS. CAN RELEASE ETHYLATED AND PROPYLATED BASES FROM DNA IN ADDITION TO 3-METHYLADENINE. TIGR00274: glucokinase regulator-related.; High confidence in function and specificity; DNA-3-methyladenine glycosylase II 2012379 alkA 4607087 alkA Azoarcus sp. BH72 DNA-3-methyladenine glycosylase II YP_933337.1 2011690 D 62928 CDS YP_933338.1 119898125 4608302 complement(2012480..2013760) 1 NC_008702.1 Probable NADH dehydrogenase. Homology to ndh of P. fluorescens of 45% (trembl|Q9KGX09 TRANSFER OF ELECTRONS FROM NADH TO THE RESPIRATORY CHAIN. THE IMMEDIATE ELECTRON ACCEPTOR FOR THE ENZYME IS BELIEVED TO BE UBIQUINONE. DOES NOT COUPLE THE REDOX REACTION TO PROTON TRANSLOCATION. InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327) Pfam: Pyridine nucleotide-disulphide oxidoreductase no signal peptide no TMHs; High confidence in function and specificity; NADH dehydrogenase 2013760 ndh2 4608302 ndh2 Azoarcus sp. BH72 NADH dehydrogenase YP_933338.1 2012480 R 62928 CDS YP_933339.1 119898126 4609349 complement(2013981..2014892) 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 2014892 4609349 azo1835 Azoarcus sp. BH72 LysR family transcriptional regulator YP_933339.1 2013981 R 62928 CDS YP_933340.1 119898127 4607088 2015262..2016950 1 NC_008702.1 Hypothetical protein sharing 76% similarity(61% identity) with Hypothetical protein [Thauera aromatica]TrEMBl O87938. Has pfam06980, DUF1302 domain,Protein of unknown function (DUF1302). This family contains a number of hypothetical bacterial proteins of unknown function that are approximately 600 residues long. Most family members seem to be from Pseudomonas.(IPR010727) No Signal peptide or TMH being reported to be present.; Function unclear; hypothetical protein 2016950 4607088 azo1836 Azoarcus sp. BH72 hypothetical protein YP_933340.1 2015262 D 62928 CDS YP_933341.1 119898128 4607089 2016980..2018332 1 NC_008702.1 Conserved hypothetical secreed protein. Homology to PA3081 of P.aeruginosa of 40% (tremble:Q9HZC7). Has DUF1329 domain(PF07044,IPRO10752). Protein of unknown function (DUF1329):This family consists of several hypothetical bacterial proteins of around 475 residues in length. The majority of family members are from Pseudomonas species but the family also contains sequences from Shewanella oneidensis and Thauera aromatica. No TMHs. Signal present.; Conserved hypothetical protein; hypothetical protein 2018332 4607089 azo1837 Azoarcus sp. BH72 hypothetical protein YP_933341.1 2016980 D 62928 CDS YP_933342.1 119898129 4607090 2018425..2019552 1 NC_008702.1 Conserved hypothetical BNR domain protein. Homology to pp3201 of P. putida of 46% (trembl|Q88I01). InterPro: BNR repeat (IPR002860). Pfam: BNR repeat. BNR repeats are short repeats never found closer than 40 residues together, which suggests that the repeat is structurally longer. These repeats are found in many glycosyl hydrolases as well as other extracellular proteins of unknown function. singal peptide. no TMHs; Function unclear; hypothetical protein 2019552 4607090 azo1838 Azoarcus sp. BH72 hypothetical protein YP_933342.1 2018425 D 62928 CDS YP_933343.1 119898130 4607091 2019552..2021942 1 NC_008702.1 Putative membrane protein MJ1562. TREMBL:Q88I00: 66% identity, 51% similarity The sterol-sensing domain (SSD) consists of approximately 180 amino acids organized into a cluster of five consecutive membrane-spanning domains and is found in proteins which have key roles in different aspects of cholesterol homeostasis or cholesterol-linked signalling such as sterol-regulated movement or the trafficking of specific cargoes. InterPro:IPR000731; SSD_5TM Found in :IPR003392: Patched family IPR004816: Hydroxymethylglutaryl-CoA reductase (NADPH Pfam:Patched:Patched family TIGRFAM:2A060602:transmembrane receptor Patch 2A0604s01: protein-export membrane pro TMHMM predicted 12 transmembrane helices; Family membership; transporter 2021942 4607091 azo1839 Azoarcus sp. BH72 transporter YP_933343.1 2019552 D 62928 CDS YP_933344.1 119898131 4607092 2021961..2022410 1 NC_008702.1 Putative universal stress protein,; Conserved hypothetical protein; putative universal stress protein 2022410 4607092 azo1840 Azoarcus sp. BH72 putative universal stress protein YP_933344.1 2021961 D 62928 CDS YP_933345.1 119898132 4607093 2022415..2023890 1 NC_008702.1 Methyl-accepting chemotaxis protein,; Specificity unclear; methyl-accepting chemotaxis transducer 2023890 4607093 azo1841 Azoarcus sp. BH72 methyl-accepting chemotaxis transducer YP_933345.1 2022415 D 62928 CDS YP_933346.1 119898133 4607094 2024002..2025687 1 NC_008702.1 Transcriptional regulatory protein lapR. In Pseudomonas sp. strain KL28 the genes, designated as lap (for long-chain alkylphenols), encoding enzymes for the catabolic 3- and 4-alkylphenol degradation pathway. The lap genes are located in a 13.2 kb region with 14 ORFs in the order lapRBKLMNOPCEHIFG and with the same transcriptional orientation. The lapR gene is transcribed independently and encodes a member of the XylR/DmpR positive transcriptional regulators. Similarity to SWISSPROT: sprot|XYLR_PSEPU (45% Pseudomonas putida, XylR) / TREMBL: trembl|Q7WYF6 (63% Pseudomonas strain KL28,LapR) InterPro: IPR002078 Sig54_interact. IPR004096 V4R. IPR003593 AAA_ATPase. IPR002197 HTH_Fis. Pfam: PF02954 HTH_8. PF00158 Sigma54_activat. PF02830 V4R. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; transcriptional regulator 2025687 lapR 4607094 lapR Azoarcus sp. BH72 transcriptional regulator YP_933346.1 2024002 D 62928 CDS YP_933347.1 119898134 4609123 2026337..2026654 1 NC_008702.1 plant type ferredoxin like protein cofactor: binds 1 2fe-2s cluster (by similarity). Pfam: fer2 2Fe-2S iron-sulfur cluster binding do; Function unclear; plant type ferredoxin like protein 2026654 4609123 azo1843 Azoarcus sp. BH72 plant type ferredoxin like protein YP_933347.1 2026337 D 62928 CDS YP_933348.1 119898135 4607095 2026691..2027626 1 NC_008702.1 In Pseudomonas sp. strain KL28 the genes,designated as lap (for long-chain alkylphenols), encoding enzymes for the catabolic 3- and 4-alkylphenol degradation pathway. The lap genes are located in a 13.2 kb region with 14 ORFs in the order lapRBKLMNOPCEHIFG and with the same transcriptional orientation. Catechol 2,3-dioxygenase LapB,; High confidence in function and specificity; catechol 2,3-dioxygenase 2027626 lapB1 4607095 lapB1 Azoarcus sp. BH72 catechol 2,3-dioxygenase YP_933348.1 2026691 D 62928 CDS YP_933349.1 119898136 4609109 2027628..2027888 1 NC_008702.1 Phenol 2-monooxygenase p0 component,; High confidence in function and specificity; phenol 2-monooxygenase 2027888 lapK 4609109 lapK Azoarcus sp. BH72 phenol 2-monooxygenase YP_933349.1 2027628 D 62928 CDS YP_933350.1 119898137 4609117 2027934..2028926 1 NC_008702.1 Phenol hydroxylase p1 protein. Catabolizes phenol and some of its methylated derivates. P1 is required for growth on phenol and for in vitro phenol hydroxylase activity. Similar to SWISSPROT: sprot|DMPL_PSESP (52% Pseudomonas sp. (strain CF600), DmpL) / TREMBL: trembl|Q7WYF3 (67% Pseudomonas sp. KL28, LapL) InterPro: IPR003430 Phenol_Hydrox. Pfam: PF02332 Methane/Phenol/Toluene Hydroxylase. Part of the lap operon: In Pseudomonas sp. strain KL28 the genes,designated as lap (for long-chain alkylphenols), encoding enzymes for the catabolic 3- and 4-alkylphenol degradation pathway. The lap genes are located in a 13.2 kb region with 14 ORFs in the order lapRBKLMNOPCEHIFG and with the same transcriptional orientation.; High confidence in function and specificity; phenol 2-monooxygenase subunit p1 2028926 lapL 4609117 lapL Azoarcus sp. BH72 phenol 2-monooxygenase subunit p1 YP_933350.1 2027934 D 62928 CDS YP_933351.1 119898138 4609118 2028935..2029204 1 NC_008702.1 Phenol 2-monooxygenase p2 component,; High confidence in function and specificity; phenol 2-monooxygenase subunit p2 2029204 lapM 4609118 lapM Azoarcus sp. BH72 phenol 2-monooxygenase subunit p2 YP_933351.1 2028935 D 62928 CDS YP_933352.1 119898139 4609119 2029221..2030738 1 NC_008702.1 Phenol 2-monooxygenase p3 component,; High confidence in function and specificity; phenol 2-monooxygenase subunit p3 2030738 lapN 4609119 lapN Azoarcus sp. BH72 phenol 2-monooxygenase subunit p3 YP_933352.1 2029221 D 62928 CDS YP_933353.1 119898140 4609120 2030817..2031173 1 NC_008702.1 Phenol 2-monooxygenase p4 component,; High confidence in function and specificity; phenol 2-monooxygenase subunit p4 2031173 lapO 4609120 lapO Azoarcus sp. BH72 phenol 2-monooxygenase subunit p4 YP_933353.1 2030817 D 62928 CDS YP_933354.1 119898141 4609121 2031229..2032290 1 NC_008702.1 Phenol hydroxylase P5 protein (EC 1.14.13.7) (Phenol 2-monooxygenase P5 component). Probable electron transfer from NADPH via FAD and the 2Fe-2S center to the oxygenase activity site of the enzyme.; High confidence in function and specificity; phenol 2-monooxygenase 2032290 lapP 4609121 lapP Azoarcus sp. BH72 phenol 2-monooxygenase YP_933354.1 2031229 D 62928 CDS YP_933355.1 119898142 4609122 complement(2032562..2033650) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Vibrio vulnificus of 41% (gi|51242695|gb|AAT99285.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2033650 4609122 azo1851 Azoarcus sp. BH72 hypothetical protein YP_933355.1 2032562 R 62928 CDS YP_933356.1 119898143 4607096 2034874..2036334 1 NC_008702.1 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase,; High confidence in function and specificity; 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase 2036334 lapC 4607096 lapC Azoarcus sp. BH72 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase YP_933356.1 2034874 D 62928 CDS YP_933357.1 119898144 4609111 2036713..2037510 1 NC_008702.1 Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy-2-oxopentanoate, involved in the bacterial meta-cleavage pathways for degradation of aromatic compounds. Belongs to the todj/xyli/xylj/hpcg family, TREMBL:Q93JW5 (62% identity); SWISSPROT:P23107 (58% identity). Pfam (PF01689): Hydratase/decarboxylase.; High confidence in function and specificity; 2-oxopent-4-enoate hydratase 2037510 lapE 4609111 lapE Azoarcus sp. BH72 2-oxopent-4-enoate hydratase YP_933357.1 2036713 D 62928 CDS YP_933358.1 119898145 4609112 2037507..2038310 1 NC_008702.1 Conversion of 4-oxalocrotonate into 2-oxopent-4-enoate, involved in the bacterial meta-cleavage pathways for degradation of aromatic compounds. Belongs to the todJ/xylI/xylJ/hpcG family, TREMBL:Q8RQD3 (56% identity); SWISSPROT:P23107. Pfam (PF01689): Hydratase/decarboxylase.; High confidence in function and specificity; 4-oxalocrotonate decarboxylase 2038310 lapH 4609112 lapH Azoarcus sp. BH72 4-oxalocrotonate decarboxylase YP_933358.1 2037507 D 62928 CDS YP_933359.1 119898146 4609115 2038330..2038551 1 NC_008702.1 4-oxalocrotonate isomerase,; High confidence in function and specificity; oxaloacetate tautomerase 2038551 lapI 4609115 lapI Azoarcus sp. BH72 oxaloacetate tautomerase YP_933359.1 2038330 D 62928 CDS YP_933360.1 119898147 4609116 2038548..2039462 1 NC_008702.1 catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase 2039462 lapF 4609116 lapF Azoarcus sp. BH72 acetaldehyde dehydrogenase YP_933360.1 2038548 D 62928 CDS YP_933361.1 119898148 4609113 2039462..2040505 1 NC_008702.1 catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase 2040505 lapG 4609113 lapG Azoarcus sp. BH72 4-hydroxy-2-ketovalerate aldolase YP_933361.1 2039462 D 62928 CDS YP_933362.1 119898149 4609114 2041184..2042116 1 NC_008702.1 Catechol 2,3-dioxygenase XylE catalyzes the ring cleavage of catechol and some substituted catechols. Similar to pir|JE0112 (52%), to trembl|Q45459 (48%)and to sprot|XYE2_PSEPU (50%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily ProDom (PD000977): Extradiol ring-cleavage dioxygenase; Specificity unclear; catechol 2,3-dioxygenase 2042116 xylE 4609114 xylE Azoarcus sp. BH72 catechol 2,3-dioxygenase YP_933362.1 2041184 D 62928 CDS YP_933363.1 119898150 4610133 2042142..2042897 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q82N83 (22% identity); TREMBL:Q9F8V0 (21% identity). Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 2042897 4610133 azo1859 Azoarcus sp. BH72 short-chain dehydrogenase YP_933363.1 2042142 D 62928 CDS YP_933364.1 119898151 4607097 2042908..2043327 1 NC_008702.1 Putative tautomerase Weak Homology with others similar proteins. Has 2 copies of PF01361, Tautomerase enzyme;IPR004370, Taut;This family includes the enzyme 4-oxalocrotonate tautomerase Q01468 that catalyses the ketonisation of 2-hydroxymuconate to 2-oxo-3-hexenedioate.; putative tautomerase 2043327 4607097 azo1860 Azoarcus sp. BH72 putative tautomerase YP_933364.1 2042908 D 62928 CDS YP_933365.1 119898152 4607098 complement(2043439..2044947) 1 NC_008702.1 Hypothetical protein. No homology of the entire protein to the data base. Has (IPR002790)PF01936;Protein of unknown function DUF88; This highly conserved bacterial protein has no known function. The alignment contains many conserved aspartates, suggesting an enzymatic function such as an endonuclease or glycosyl hydrolase (Bateman A pers. obs). No Signal peptide or TMH present.; hypothetical protein 2044947 4607098 azo1861 Azoarcus sp. BH72 hypothetical protein YP_933365.1 2043439 R 62928 CDS YP_933366.1 119898153 4607099 2045147..2045857 1 NC_008702.1 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase). Responsible for synthesis of pseudouridine from uracil-746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs.; Family membership; pseudouridine synthase A 2045857 rluA2 4607099 rluA2 Azoarcus sp. BH72 pseudouridine synthase A YP_933366.1 2045147 D 62928 CDS YP_933367.1 119898154 4610007 2046178..2047254 1 NC_008702.1 Integrase. INTEGRASE IS NECESSARY FOR INTEGRATION OF THE PHAGE INTO THE HOST GENOME BY SITE-SPECIFIC RECOMBINATION. IN CONJUNCTION WITH EXCISIONASE INTEGRASE IS ALSO NECESSARY FOR EXCISION OF THE PROPHAGE FROM THE HOST GENOME.; Specificity unclear; phage integrase family site specific recombinase 2047254 4610007 azo1863 Azoarcus sp. BH72 phage integrase family site specific recombinase YP_933367.1 2046178 D 62928 CDS YP_933368.1 119898155 4607100 2047997..2048656 1 NC_008702.1 Hypothetical protein , 27% identity to TrEMBL;Q84EQ6. No domains, repeats, motifs or features present.; hypothetical protein 2048656 4607100 azo1864 Azoarcus sp. BH72 hypothetical protein YP_933368.1 2047997 D 62928 CDS YP_933369.1 119898156 4607101 2048746..2049024 1 NC_008702.1 Hypothetical protein. No matches in the database. No domains, repeats, motifs or features present.; hypothetical protein 2049024 4607101 azo1865 Azoarcus sp. BH72 hypothetical protein YP_933369.1 2048746 D 62928 CDS YP_933370.1 119898157 4607102 2049041..2049337 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2049337 4607102 azo1866 Azoarcus sp. BH72 hypothetical protein YP_933370.1 2049041 D 62928 CDS YP_933371.1 119898158 4607103 2049341..2049763 1 NC_008702.1 Hypothetical protein. Very weak homology with hits in the database. No domains, repeats, motifs or features present.; hypothetical protein 2049763 4607103 azo1867 Azoarcus sp. BH72 hypothetical protein YP_933371.1 2049341 D 62928 CDS YP_933372.1 119898159 4607104 2050193..2050453 1 NC_008702.1 Hypothetical protein, very weak homology with hits in the Database. No Motifs,domains, or Signal peptide present.; hypothetical protein 2050453 4607104 azo1868 Azoarcus sp. BH72 hypothetical protein YP_933372.1 2050193 D 62928 CDS YP_933373.1 119898160 4607105 2050453..2050995 1 NC_008702.1 Putative Hypothetical protein. Very weak homology with hits in the Database. No signficant domains, motifs or Signal Peptide present.; hypothetical protein 2050995 4607105 azo1869 Azoarcus sp. BH72 hypothetical protein YP_933373.1 2050453 D 62928 CDS YP_933374.1 119898161 4607106 2050956..2052833 1 NC_008702.1 Putative protease encoded ATP-dependent Clp protease family protein similar(30 Identity,45% similarity in function) to TrEMBL:Q8X866. IPR001907. Has pfam00574,CLP_protease, Clp protease. The Clp protease has an active site catalytic triad. In E. coli Clp protease, ser-111,his-136 and asp-185 form the catalytic triad. One member has lost all of these active site residues and is therefore inactive. Some members contain one or two large insertions. Peptidase family S49 domain present. Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). PF00574;CLP_protease PR00127;CLPPROTEASEP PS00381;CLP_PROTEASE_SER PS00382;CLP_PROTEASE_HIS TIGR00493;clpP No Signal peptide or TMH being reported as present.; High confidence in function and specificity; endopeptidase Clp 2052833 4607106 azo1870 Azoarcus sp. BH72 endopeptidase Clp YP_933374.1 2050956 D 62928 CDS YP_933375.1 119898162 4607107 2052959..2053276 1 NC_008702.1 hypothetical protein 2053276 4607107 azo1871 Azoarcus sp. BH72 hypothetical protein YP_933375.1 2052959 D 62928 CDS YP_933376.1 119898163 4607108 2053263..2055575 1 NC_008702.1 Hypothetical phage tail tape measure protein. Controls phage tail length no signal peptide. no TMH; phage tail tape measure protein 2055575 4607108 azo1872 Azoarcus sp. BH72 phage tail tape measure protein YP_933376.1 2053263 D 62928 CDS YP_933377.1 119898164 4607109 2055575..2056312 1 NC_008702.1 Hypothetical protein, 26% Identity to TrEMBL;Q9PFZ1. No domains, repeats, motifs or features could be predicted above threshold.; hypothetical protein 2056312 4607109 azo1873 Azoarcus sp. BH72 hypothetical protein YP_933377.1 2055575 D 62928 CDS YP_933378.1 119898165 4607110 complement(2056922..2057467) 1 NC_008702.1 dTDP-4-dehydrorhamnose 35-epimerase (EC 5.1.3.13) (dTDP-4-keto-6- deoxyglucose 35-epimerase) (dTDP-L-rhamnose synthetase). InterPro: dTDP-4-dehydrorhamnose 35-epimerase; High confidence in function and specificity; dTDP-4-dehydrorhamnose 3,5-epimerase 2057467 rmlC 4607110 rmlC Azoarcus sp. BH72 dTDP-4-dehydrorhamnose 3,5-epimerase YP_933378.1 2056922 R 62928 CDS YP_933379.1 119898166 4609737 complement(2057467..2058351) 1 NC_008702.1 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase).; High confidence in function and specificity; glucose-1-phosphate thymidylyltransferase 2058351 rmlA 4609737 rmlA Azoarcus sp. BH72 glucose-1-phosphate thymidylyltransferase YP_933379.1 2057467 R 62928 CDS YP_933380.1 119898167 4609735 complement(2058363..2059253) 1 NC_008702.1 Region start changed from 2059335 to 2059254 (-81 bases); dTDP-4-dehydrorhamnose reductase 2059253 4609735 azo1876 Azoarcus sp. BH72 dTDP-4-dehydrorhamnose reductase YP_933380.1 2058363 R 62928 CDS YP_933381.1 119898168 4607111 complement(2059250..2060326) 1 NC_008702.1 dTDP-glucose 46-dehydratase (EC 4.2.1.46). InterPro: NAD dependent epimerase/dehydratase family; High confidence in function and specificity; dTDP-glucose 4,6-dehydratase 2060326 rmlB 4607111 rmlB Azoarcus sp. BH72 dTDP-glucose 4,6-dehydratase YP_933381.1 2059250 R 62928 CDS YP_933382.1 119898169 4609736 2060555..2061478 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pp4172 of P. putida of 33% (trembl|Q88FC6). Pfam: DUF534 This is a family of putative secreted proteins of unknown function. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2061478 4609736 azo1878 Azoarcus sp. BH72 hypothetical protein YP_933382.1 2060555 D 62928 CDS YP_933383.1 119898170 4607112 2061475..2063547 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to Daro03001110 of Dechloromonas aromatica of 33% (gi|46141071|ref|ZP_00203851.1|(NBCI ENTREZ)). InterPro: IPR003660 HAMP. IPR000160 GGDEF. Pfam: PF00563 EAL domain. PF00672 HAMP. PF00990 GGDEF domain. TIGRFAM:TIGR00254 putative diguanylate cyclase (GGDEF) domain. Signal P reporting signal peptide. TMHMM reporting 3 transmembrane helices.; Conserved hypothetical protein; putative signaling protein 2063547 4607112 azo1879 Azoarcus sp. BH72 putative signaling protein YP_933383.1 2061475 D 62928 CDS YP_933384.1 119898171 4607113 complement(2063554..2064687) 1 NC_008702.1 Conserved hypothetical secreted protein. TREMBL:O86370 (36% identity); TREMBL:Q7U0W8 (36% identity). No domains predicted. SignalP reporting signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 2064687 4607113 azo1880 Azoarcus sp. BH72 hypothetical protein YP_933384.1 2063554 R 62928 CDS YP_933385.1 119898172 4607114 complement(2064687..2067200) 1 NC_008702.1 Similar to TREMBL:Q82US4 (29% identity). Pfam (DUF214): Predicted permease. TMHMM predicting 10 transmembrane helices.; Specificity unclear; ABC transporter permease 2067200 4607114 azo1881 Azoarcus sp. BH72 ABC transporter permease YP_933385.1 2064687 R 62928 CDS YP_933386.1 119898173 4607115 complement(2067197..2067874) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q89X55 (42% identity); SWISSPROT:P31219 (40% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR003593): AAA ATPase. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). Pfam (PF00005): ABC transporter.; Family membership; ABC transporter ATP-binding protein 2067874 4607115 azo1882 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_933386.1 2067197 R 62928 CDS YP_933387.1 119898174 4607116 2067967..2068812 1 NC_008702.1 Putative phytoene synthase. Homology to crtB of Synechococcus sp. of 30% (SWISSPROT:CRTB_SYNP7 Is involved in the synthesis of carotenoids. Catalyzes the reaction from prephytoene diphosphate to phytoene. Pfam: Squalene/phytoene synthase no singal peptide no TMHs; Family membership; putative phytoene synthase 2068812 crtB1 4607116 crtB1 Azoarcus sp. BH72 putative phytoene synthase YP_933387.1 2067967 D 62928 CDS YP_933388.1 119898175 4608534 2068913..2069746 1 NC_008702.1 Putative phytoene synthase. Homology to crtB of Synechococcus sp. of 35% (SWISSPROT:CRTB_SYNP7 Is involved in the synthesis of carotenoids. Catalyzes the reaction from prephytoene diphosphate to phytoene. Pfam: Squalene/phytoene synthase no singal peptide no TMHs; Family membership; putative phytoene synthase 2069746 crtB2 4608534 crtB2 Azoarcus sp. BH72 putative phytoene synthase YP_933388.1 2068913 D 62928 CDS YP_933389.1 119898176 4608535 2069751..2071058 1 NC_008702.1 Putative oxidoreductase precursor, 27% identity (38% similarity) to TrEMBL;Q6N3F3. TrEMBL;Q9RI54. Has PF00070;Pyridine nucleotide-disulphide oxidoreductase; IPR001327 FAD_pyr_redox:This family includes both class I and class II oxidoreductases and also NADH oxidases and peroxidases. This domain is actually a small NADH binding domain within a larger FAD binding domain. Has PF01593,Flavin containing amine oxidoreductase;(IPR002937,Amino_oxidase):This family consists of various amine oxidases, including maze polyamine oxidase (PAO)and various flavin containing monoamine oxidases (MAO). The family also contains phytoene dehydrogenases and related enzymes. In vertebrates MAO plays an important role regulating the intracellular levels of amines via there oxidation; these include various neurotransmitters,neurotoxins and trace amines. In lower eukaryotes such as aspergillus and in bacteria the main role of amine oxidases is to provide a source of ammonium. PAOs in plants, bacteria and protozoa oxidase spermidine and spermine to an aminobutyral, diaminopropane and hydrogen peroxide and are involved in the catabolism of polyamines. Has PF01494, FAD binding domain;(IPR002938 Moxy_FAD_binding);This domain is involved in FAD binding in a number of enzymes.; Family membership; putative oxidoreductase 2071058 4608535 azo1885 Azoarcus sp. BH72 putative oxidoreductase YP_933389.1 2069751 D 62928 CDS YP_933390.1 119898177 4607117 complement(2071071..2072195) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc2008 of R. solanacearum of 59% (trembl|Q8XXV5). no domain predicted. no signal peptide. no TMHs; hypothetical protein 2072195 4607117 azo1886 Azoarcus sp. BH72 hypothetical protein YP_933390.1 2071071 R 62928 CDS YP_933391.1 119898178 4607118 complement(2072225..2073010) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS03577 of R.solanacearum of 66% (tremble:Q8XXV4). Has PF03883,Protein of unknown function (DUF328); IPR005583 ;Members of this family are functionally uncharacterised. They are about 250 amino acids in length. No signal peptide. No TMHs; hypothetical protein 2073010 4607118 azo1887 Azoarcus sp. BH72 hypothetical protein YP_933391.1 2072225 R 62928 CDS YP_933392.1 119898179 4607119 2073125..2074477 1 NC_008702.1 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) (7,8-diamino-pelargonic acid aminotransferase) (DAPA aminotransferase). TGIRFAM: bioA: adenosylmethionine--8-amino-7-oxononanoate transaminase; High confidence in function and specificity; adenosylmethionine-8-amino-7-oxononanoate aminotransferase 2074477 bioA 4607119 bioA Azoarcus sp. BH72 adenosylmethionine-8-amino-7-oxononanoate aminotransferase YP_933392.1 2073125 D 62928 CDS YP_933393.1 119898180 4608389 2074477..2075649 1 NC_008702.1 8-amino-7-oxononanoate synthase (EC 2.3.1.47) (AONS) (8-amino-7- ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase). InterPro: Aminotransferases class-I bioF: 8-amino-7-oxononanoate synthase; High confidence in function and specificity; 8-amino-7-oxononanoate synthase 2075649 bioF 4608389 bioF Azoarcus sp. BH72 8-amino-7-oxononanoate synthase YP_933393.1 2074477 D 62928 CDS YP_933394.1 119898181 4608394 2075642..2076406 1 NC_008702.1 BioH protein.Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily). Seems to be implicated in the early steps of biotin biosynthesis.; Function unclear; BioH protein 2076406 bioH 4608394 bioH Azoarcus sp. BH72 BioH protein YP_933394.1 2075642 D 62928 CDS YP_933395.1 119898182 4608395 2076399..2077193 1 NC_008702.1 Biotin synthesis protein bioC. bioc is involved in an early, but chemically unexplored, step in the conversion of pimelic acid to biotin.; Specificity unclear; biotin synthesis protein 2077193 bioC1 4608395 bioC1 Azoarcus sp. BH72 biotin synthesis protein YP_933395.1 2076399 D 62928 CDS YP_933396.1 119898183 4608391 2077190..2077876 1 NC_008702.1 DTB synthetase; dethiobiotin synthase; involved in production of dethiobiotin from ATP and 7,8-diaminononanoate and carbon dioxide; contains magnesium; dithiobiotin synthetase 2077876 bioD 4608391 bioD Azoarcus sp. BH72 dithiobiotin synthetase YP_933396.1 2077190 D 62928 CDS YP_933397.1 119898184 4608393 complement(2077881..2078672) 1 NC_008702.1 Region start changed from 2079087 to 2078673 (-414 bases); RNA methyltransferase 2078672 4608393 azo1893 Azoarcus sp. BH72 RNA methyltransferase YP_933397.1 2077881 R 62928 CDS YP_933398.1 119898185 4607120 complement(2078681..2079136) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA1044 of P. aeruginosa of 44% (trembl|Q9I4T0). No domains predicted. Signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 2079136 4607120 azo1894 Azoarcus sp. BH72 hypothetical protein YP_933398.1 2078681 R 62928 CDS YP_933399.1 119898186 4607121 complement(2079133..2079747) 1 NC_008702.1 RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids; ribonuclease HII 2079747 rnhB 4607121 rnhB Azoarcus sp. BH72 ribonuclease HII YP_933399.1 2079133 R 62928 CDS YP_933400.1 119898187 4609754 complement(2079720..2080895) 1 NC_008702.1 catalyzes the formation of lipid A disaccharide from UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is a precursor of lipid A that anchors LPS to the OM; lipid-A-disaccharide synthase 2080895 lpxB 4609754 lpxB Azoarcus sp. BH72 lipid-A-disaccharide synthase YP_933400.1 2079720 R 62928 CDS YP_933401.1 119898188 4609175 complement(2080900..2081670) 1 NC_008702.1 catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis; UDP-N-acetylglucosamine acyltransferase 2081670 lpxA 4609175 lpxA Azoarcus sp. BH72 UDP-N-acetylglucosamine acyltransferase YP_933401.1 2080900 R 62928 CDS YP_933402.1 119898189 4609174 complement(2081667..2082104) 1 NC_008702.1 in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP; (3R)-hydroxymyristoyl-ACP dehydratase 2082104 fabZ 4609174 fabZ Azoarcus sp. BH72 (3R)-hydroxymyristoyl-ACP dehydratase YP_933402.1 2081667 R 62928 CDS YP_933403.1 119898190 4608716 complement(2082110..2083135) 1 NC_008702.1 adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase 2083135 lpxD 4608716 lpxD Azoarcus sp. BH72 UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase YP_933403.1 2082110 R 62928 CDS YP_933404.1 119898191 4609177 complement(2083149..2083649) 1 NC_008702.1 Probable outer membrane protein. Homology to ompH of Y. enterocolitica of 30% (sprot|OMPH_YEREN). Pfam: outer membrane protein (OmpH-like) signal peptide no TMHs; High confidence in function and specificity; outer membrane protein 2083649 ompH 4609177 ompH Azoarcus sp. BH72 outer membrane protein YP_933404.1 2083149 R 62928 CDS YP_933405.1 119898192 4609445 complement(2083683..2085962) 1 NC_008702.1 InterPro: Bacterial surface antigen (D15); Function unclear; outer membrane protein 2085962 oma 4609445 oma Azoarcus sp. BH72 outer membrane protein YP_933405.1 2083683 R 62928 CDS YP_933406.1 119898193 4609441 complement(2086001..2087365) 1 NC_008702.1 Conserved hypothetical membrane-associated zinc metalloprotease. Homology to ne1711 of N. europaea of 51% (trembl|Q82U02). Tigrfam: TIGR00054: membrane-associated zinc metalloprotease. Pfam: PDZ domain (Also known as DHR or GLGF). no signal peptide. 4 TMHs; Family membership; membrane-associated zinc metalloprotease 2087365 4609441 azo1902 Azoarcus sp. BH72 membrane-associated zinc metalloprotease YP_933406.1 2086001 R 62928 CDS YP_933407.1 119898194 4607122 complement(2087365..2088564) 1 NC_008702.1 catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate; 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2088564 dxr 4607122 dxr Azoarcus sp. BH72 1-deoxy-D-xylulose 5-phosphate reductoisomerase YP_933407.1 2087365 R 62928 CDS YP_933408.1 119898195 4608658 complement(2088581..2089405) 1 NC_008702.1 Phosphatidate cytidylyltransferase :- catalytic activity ctp + phosphatidate = diphosphate + cdp-diacylglycerol. pathway :-phospholipid biosynthesis. Entry name:- CDSA_ECOLI Primary accession number :- P06466 InterPro:- IPR000374; PC_trans. Identities = 89/252 (35%) Pfam PF01148; CTP_transf_1; 1. Number of predicted TMHs: 7 Prediction: Signal peptide Signal peptide probability: 1.0; Family membership; putative phosphatidate cytidylyltransferase 2089405 cdsA 4608658 cdsA Azoarcus sp. BH72 putative phosphatidate cytidylyltransferase YP_933408.1 2088581 R 62928 CDS YP_933409.1 119898196 4608461 complement(2089398..2090198) 1 NC_008702.1 Undecaprenyl pyrophosphate synthetase :- Generates undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP). UPP is the precursor of the carrier lipid for peptidoglycan synthesis (By similarity). Identities = 124/228 (54%) Entry name SWISSPROT:UPPS_ECOLI Prim. accession # P60472 InterPro :- IPR001441; UPP_synth. Pfam:- PF01255; UPP_synthetase; 1. Number of predicted TMHs: 0 Prediction: Non-secretory protein Signal peptide probability: 0.0; Family membership; undecaprenyl pyrophosphate synthetase 2090198 uppS 4608461 uppS Azoarcus sp. BH72 undecaprenyl pyrophosphate synthetase YP_933409.1 2089398 R 62928 CDS YP_933410.1 119898197 4610070 complement(2090246..2090803) 1 NC_008702.1 Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs; ribosome recycling factor 2090803 frr 4610070 frr Azoarcus sp. BH72 ribosome recycling factor YP_933410.1 2090246 R 62928 CDS YP_933411.1 119898198 4608830 complement(2090814..2091530) 1 NC_008702.1 Catalyzes the phosphorylation of UMP to UDP; uridylate kinase 2091530 pyrH 4608830 pyrH Azoarcus sp. BH72 uridylate kinase YP_933411.1 2090814 R 62928 CDS YP_933412.1 119898199 4609674 complement(2091541..2092434) 1 NC_008702.1 EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu; elongation factor Ts 2092434 tsf 4609674 tsf Azoarcus sp. BH72 elongation factor Ts YP_933412.1 2091541 R 62928 CDS YP_933413.1 119898200 4610045 complement(2092546..2093301) 1 NC_008702.1 one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit; 30S ribosomal protein S2 2093301 rpsB 4610045 rpsB Azoarcus sp. BH72 30S ribosomal protein S2 YP_933413.1 2092546 R 62928 CDS YP_933414.1 119898201 4607123 2093581..2094399 1 NC_008702.1 catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn; methionine aminopeptidase 2094399 map 4607123 map Azoarcus sp. BH72 methionine aminopeptidase YP_933414.1 2093581 D 62928 CDS YP_933415.1 119898202 4609194 2094419..2097004 1 NC_008702.1 catalyzes the uridylylation or deuridylylation of the PII nitrogen regulatory protein; PII uridylyl-transferase 2097004 glnD 4609194 glnD Azoarcus sp. BH72 PII uridylyl-transferase YP_933415.1 2094419 D 62928 CDS YP_933416.1 119898203 4608896 complement(2097065..2097700) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RPA0231 of Rhodopseudomonas palustris of 39% (trembl|Q6ND78). Has PF03350,(IPR005134) Uncharacterized protein family, UPF0114: This conserved hypothetical protein family with four predicted transmembrane regions is found in E. coli, Haemophilus influenzae, and Helicobacter pylori, among completed genomes. No signal peptide. 3 TMHs; hypothetical protein 2097700 4608896 azo1912 Azoarcus sp. BH72 hypothetical protein YP_933416.1 2097065 R 62928 CDS YP_933417.1 119898204 4607124 2097850..2099463 1 NC_008702.1 Conserved hypothetical protein. Homology to pp1134 of P. putida of 77% (trembl|Q88NS1). Pfam: Phytoene dehydrogenase related enzyme. no signal peptide. no TMHs; hypothetical protein 2099463 4607124 azo1913 Azoarcus sp. BH72 hypothetical protein YP_933417.1 2097850 D 62928 CDS YP_933418.1 119898205 4607125 complement(2099467..2100810) 1 NC_008702.1 C4-dicarboxylate transport transcriptional regulatory protein,; High confidence in function and specificity; transcriptional regulator 2100810 dctD2 4607125 dctD2 Azoarcus sp. BH72 transcriptional regulator YP_933418.1 2099467 R 62928 CDS YP_933419.1 119898206 4608596 complement(2100821..2102704) 1 NC_008702.1 C4-dicarboxylate transport sensor protein,; High confidence in function and specificity; sensor histidine kinase 2102704 dctB2 4608596 dctB2 Azoarcus sp. BH72 sensor histidine kinase YP_933419.1 2100821 R 62928 CDS YP_933420.1 119898207 4608594 complement(2102656..2103072) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to blr1303 of B. japonicum of 38% (trembl|Q89UV6(SRS)) No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 2103072 4608594 azo1916 Azoarcus sp. BH72 hypothetical protein YP_933420.1 2102656 R 62928 CDS YP_933421.1 119898208 4607126 complement(2103072..2105108) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to so0455 of S. oneidensis of 65% (trembl|Q8EJK8). Tigrfam: dctM: TRAP dicarboxylate transporter-DctM subunit. Pfam: TRAP C4-dicarboxylate transport (Dct) system permease DctM subunit. This domain represents a conserved region located towards the N terminus of the DctM subunit of the bacterial and archaeal TRAP C4-dicarboxylate transport (Dct) system permease. no signal peptide. 17 TMHs; Conserved hypothetical protein; hypothetical protein 2105108 4607126 azo1917 Azoarcus sp. BH72 hypothetical protein YP_933421.1 2103072 R 62928 CDS YP_933422.1 119898209 4607127 complement(2105234..2106184) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pp3954 pf P. putida of 64% (trembl|Q88FX3). no domains predicted. singal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 2106184 4607127 azo1918 Azoarcus sp. BH72 hypothetical protein YP_933422.1 2105234 R 62928 CDS YP_933423.1 119898210 4607128 2106499..2107608 1 NC_008702.1 Hypothetical protein MG039 homolog (D09_orf384). D-amino acid oxidase (EC: 1.4.3.3) (DAMOX or DAO) is an FAD flavoenzyme that catalyzes the oxidation of neutral and basic D-amino acids into their corresponding keto acids. DAOs have been characterized and sequenced in fungi and vertebrates where they are known to be located in the peroxisomes. InterPro: D-amino acid oxidase Putative conserved exported protein. TREMBL:Q7WG75: 55% identity,65% similarity. TREMBLnew:CAE29609. Pfam:NAD binding FAD dependent oxidoreductase. TIGRFAM:UDP-GALP mutase. Signal P predicted signal peptide and TMHMM predicted transmembrane helices. gidA: glucose-inhibited division prot; Specificity unclear; oxidoreductase 2107608 4607128 azo1919 Azoarcus sp. BH72 oxidoreductase YP_933423.1 2106499 D 62928 CDS YP_933424.1 119898211 4607129 complement(2107621..2109078) 1 NC_008702.1 Probable succinate-semialdehyde dehydrogenase [NAD(P)+]. Homology to gabD of P. aeruginosa of 62% (trembl|Q9RBF6) Catalysis of the reaction: succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H + H+. Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; High confidence in function and specificity; succinate semialdehyde dehydrogenase 2109078 gabD2 4607129 gabD2 Azoarcus sp. BH72 succinate semialdehyde dehydrogenase YP_933424.1 2107621 R 62928 CDS YP_933425.1 119898212 4608852 complement(2109292..2110215) 1 NC_008702.1 Probable electron transfer flavoprotein, alpha subunit. Homology to etfA of B. japonicum of 66% (sprot|ETFA_BRAJA) The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein alpha-subunit (IPR001308) Pfam: Electron transfer flavoprotein alpha subunit no signal peptide no TMHs; High confidence in function and specificity; electron transfer flavoprotein subunit alpha 2110215 etfA2 4608852 etfA2 Azoarcus sp. BH72 electron transfer flavoprotein subunit alpha YP_933425.1 2109292 R 62928 CDS YP_933426.1 119898213 4608679 complement(2110306..2111055) 1 NC_008702.1 Electron transfer flavoprotein, beta subunit. Homology to etfB of B. japonicum of 71% (sprot|ETFB_BRAJA). The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). Pfam: Electron transfer flavoprotein beta subunit no signal peptide no TMHS; High confidence in function and specificity; electron transfer flavoprotein subunit beta 2111055 etfB2 4608679 etfB2 Azoarcus sp. BH72 electron transfer flavoprotein subunit beta YP_933426.1 2110306 R 62928 CDS YP_933427.1 119898214 4608683 complement(2111082..2112305) 1 NC_008702.1 Conserved hypothetical protein. Homology to pa0446 of P. aeruginosa of 72% (trembl|Q9I672). Pfam: CAIB/BAIF family. no signal peptide. no TMHs; hypothetical protein 2112305 4608683 azo1923 Azoarcus sp. BH72 hypothetical protein YP_933427.1 2111082 R 62928 CDS YP_933428.1 119898215 4607130 complement(2112858..2114045) 1 NC_008702.1 CATALYZES THE OXIDATIVE DECARBOXYLATION OF GLUTARYL-COA TO CROTONYL-COA AND CO(2) IN THE DEGRADATIVE PATHWAY OF L-LYSINE L-HYDROXYLYSINE AND L-TRYPTOPHAN METABOLISM. IT USES ELECTRON TRANSFER FLAVOPROTEIN AS ITS ELECTRON ACCEPTOR. Entry name TREMBL:Q98HG5 Prim. accession # Q98HG5 Identities = 253/397 (63%) InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; 1. Number of predicted TMHs: 0 Prediction: Non-secretory protein Signal peptide probability: 0.000; Family membership; glutaryl-CoA dehydrogenase 2114045 gcdH1 4607130 gcdH1 Azoarcus sp. BH72 glutaryl-CoA dehydrogenase YP_933428.1 2112858 R 62928 CDS YP_933429.1 119898216 4608864 2114231..2115175 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 2115175 4608864 azo1925 Azoarcus sp. BH72 LysR family transcriptional regulator YP_933429.1 2114231 D 62928 CDS YP_933430.1 119898217 4607131 2115279..2116043 1 NC_008702.1 Hypothetical protein. no homology over the entire protein. no domain structure no signal peptide no TMHs; hypothetical protein 2116043 4607131 azo1926 Azoarcus sp. BH72 hypothetical protein YP_933430.1 2115279 D 62928 CDS YP_933431.1 119898218 4607132 complement(2116100..2116894) 1 NC_008702.1 Catalysis of the reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or3)-enoyl-CoA + H2O. Entry name TREMBL:Q7VU52 Prim. accession # Q7VU52 Identities = 124/266 (46%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; High confidence in function and specificity; enoyl-CoA hydratase 2116894 4607132 azo1927 Azoarcus sp. BH72 enoyl-CoA hydratase YP_933431.1 2116100 R 62928 CDS YP_933432.1 119898219 4607133 complement(2116903..2117298) 1 NC_008702.1 Conserved hypothetical protein. Homology to orf5 of Azoarcus evansii of 78% (tremble:Q93FB4). Pfam: Endoribonuclease L-PSP. Endoribonuclease active on single-stranded mRNA. Inhibits protein synthesis by cleavage of mRNA. Previously thought to inhibit protein synthesis initiation. This protein may also be involved in the regulation of purine biosynthesis. No signal peptide. No TMHs; hypothetical protein 2117298 abmE 4607133 abmE Azoarcus sp. BH72 hypothetical protein YP_933432.1 2116903 R 62928 CDS YP_933433.1 119898220 4608251 complement(2117291..2117746) 1 NC_008702.1 TREMBL:Q89PR6: 50% identity; 71% similarity Probable 4-hydroxybenzoyl-CoA thioesterase . InterPro:IPR008272; 4HBcoA_thiost_AS Pfam:4HBT:4-hydroxybenzoyl-CoA thioesterase TIGR00051: conserved hypothetical protein No transmembrane helices; Function unclear; 4-hydroxybenzoyl CoA thioesterase 2117746 4608251 azo1929 Azoarcus sp. BH72 4-hydroxybenzoyl CoA thioesterase YP_933433.1 2117291 R 62928 CDS YP_933434.1 119898221 4607134 complement(2117761..2118942) 1 NC_008702.1 Glutaryl-CoA dehydrogenase mitochondrial precursor CATALYZES THE OXIDATIVE DECARBOXYLATION OF GLUTARYL-COA TO CROTONYL-COA AND CO(2) IN THE DEGRADATIVE PATHWAY OF L-LYSINE L-HYDROXYLYSINE AND L-TRYPTOPHAN METABOLISM. IT USES ELECTRON TRANSFER FLAVOPROTEIN AS ITS ELECTRON ACCEPTOR. Entry name TREMBL:Q88RH2 Prim. accession # Q88RH2 InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; 1. Number of predicted TMHs: 0; Family membership; glutaryl-CoA dehydrogenase 2118942 gcdH2 4607134 gcdH2 Azoarcus sp. BH72 glutaryl-CoA dehydrogenase YP_933434.1 2117761 R 62928 CDS YP_933435.1 119898222 4608865 complement(2118989..2120140) 1 NC_008702.1 Catalysis of the reaction:- acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Entry name TREMBL:Q98BT9 Prim. accession # Q98BT9 InterPro IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. Number of predicted TMHs: 0; Family membership; putative acyl-CoA dehydrogenase 2120140 abmD 4608865 abmD Azoarcus sp. BH72 putative acyl-CoA dehydrogenase YP_933435.1 2118989 R 62928 CDS YP_933436.1 119898223 4608250 complement(2120148..2120993) 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 2120993 abmC 4608250 abmC Azoarcus sp. BH72 enoyl-CoA hydratase YP_933436.1 2120148 R 62928 CDS YP_933437.1 119898224 4608249 complement(2121433..2122242) 1 NC_008702.1 Probable beta-hydroxyacyl-CoA dehydrogenase. Homology to abmB of A. evansii of 79%. InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases. InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase. No signal peptide. No TMHs.; Specificity unclear; beta-hydroxyacyl-CoA dehydrogenase 2122242 abmB 4608249 abmB Azoarcus sp. BH72 beta-hydroxyacyl-CoA dehydrogenase YP_933437.1 2121433 R 62928 CDS YP_933438.1 119898225 4608248 complement(2122523..2124838) 1 NC_008702.1 catalyzes the conversion of salicylyl-CoA to gentisyl-CoA; salicylyl-CoA 5-hydroxylase 2124838 abmA 4608248 abmA Azoarcus sp. BH72 salicylyl-CoA 5-hydroxylase YP_933438.1 2122523 R 62928 CDS YP_933439.1 119898226 4608247 complement(2124973..2125479) 1 NC_008702.1 Putative MarR-family transcriptional regulator,; Family membership; MarR family transcriptional regulator 2125479 4608247 azo1935 Azoarcus sp. BH72 MarR family transcriptional regulator YP_933439.1 2124973 R 62928 CDS YP_933440.1 119898227 4607135 2125695..2126702 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2126702 4607135 azo1936 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933440.1 2125695 D 62928 CDS YP_933441.1 119898228 4607136 2126825..2127613 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q9AQN5 (39% identity); TREMBL:Q987X7 (35% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short chain dehydrogenase 2127613 4607136 azo1937 Azoarcus sp. BH72 short chain dehydrogenase YP_933441.1 2126825 D 62928 CDS YP_933442.1 119898229 4607137 2127668..2128966 1 NC_008702.1 Putative styrene monooxygenase. Homology to styA of P. fluorescens of 25% (TREMBL:O06834) Encode a styrene monooxygenase responsible for the transformation of styrene to epoxystyrene Pfam: FAD binding domain no signal peptide no TMHs; Family membership; putative styrene monooxygenase 2128966 styA 4607137 styA Azoarcus sp. BH72 putative styrene monooxygenase YP_933442.1 2127668 D 62928 CDS YP_933443.1 119898230 4609902 2129059..2129544 1 NC_008702.1 Nitrilotriacetate monooxygenase component B (EC 1.14.13.-) (NTA monooxygenase component B) (NTA-MO B). HYDROXYLATION OF NITRILOTRIACETATE. TREMBL:Q93NA5:48% identity, 62% similarity InterPro: IPR002563; Flavin_Reduct. IPR009002; FMN_binding. Pfam:PF01613; Flavin_Reduct Non-secretory protein with low signal peptide probability (0.002) Absence of transmembrane helices; High confidence in function and specificity; monooxygenase component B 2129544 nmoB 4609902 nmoB Azoarcus sp. BH72 monooxygenase component B YP_933443.1 2129059 D 62928 CDS YP_933444.1 119898231 4609385 complement(2129896..2130807) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PP4294 of P. putida of 67% (trembl|Q88F06). Has PF05661, Protein of unknown function (DUF808);IPR008526;This family consists of several bacterial proteins of unknown function. no signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 2130807 4609385 azo1940 Azoarcus sp. BH72 hypothetical protein YP_933444.1 2129896 R 62928 CDS YP_933445.1 119898232 4607138 complement(2130993..2132576) 1 NC_008702.1 Putative glucose dehydrogenase alpha subunit. Homology to gdhAlpha of B. cepacia of 28% (trembl|Q8GQE7). InterPro: NAD binding site (IPR000205); UBA/THIF-type NAD/FAD binding fold (IPR000594), $Fe-4s ferredoxin, ion-sulfur binding domain (IPR001450) Pfam: GMC oxidoreductase no signal peptide no TMHs gid: gid protein; Function unclear; putative glucose dehydrogenase subunit alpha 2132576 gdhAlpha2 4607138 gdhAlpha2 Azoarcus sp. BH72 putative glucose dehydrogenase subunit alpha YP_933445.1 2130993 R 62928 CDS YP_933446.1 119898233 4608874 complement(2132573..2133175) 1 NC_008702.1 Hypothetical secreted protein. No good homology with hits in the database. No domains predicted. Signal peptide present. No TMHs; hypothetical protein 2133175 4608874 azo1942 Azoarcus sp. BH72 hypothetical protein YP_933446.1 2132573 R 62928 CDS YP_933447.1 119898234 4607139 complement(2133386..2133904) 1 NC_008702.1 Putative flavin:NADH reductase ycdH (EC 1.6.8.-). Catalyzes the reduction of free flavins (FMN FAD and riboflavin) by NADH (By similarity). TREMBL:Q8RLG2: 41% identity, 60% similarity InterPro: Flavin reductase-like domain InterPro: IPR002563; Flavin_Reduct. IPR009002; FMN_binding. Pfam PF01613; Flavin_Reduct gmhA: phosphoheptose isomerase No signal peptide Absence of transmembrane helices; Function unclear; HPA reductase 2133904 hpaC 4607139 hpaC Azoarcus sp. BH72 HPA reductase YP_933447.1 2133386 R 62928 CDS YP_933448.1 119898235 4609024 complement(2133901..2135031) 1 NC_008702.1 Conserved hypothetical protein. Homology to mll3445) of M. loti of 71% (trembl|Q98G8). InterPro: Bacterial luciferase (IPR002103). Pfam: Luciferase-like monooxygenase. no signal peptide. no TMHS; hypothetical protein 2135031 4609024 azo1944 Azoarcus sp. BH72 hypothetical protein YP_933448.1 2133901 R 62928 CDS YP_933449.1 119898236 4607140 complement(2135167..2136183) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2136183 4607140 azo1945 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933449.1 2135167 R 62928 CDS YP_933450.1 119898237 4607141 2136465..2139191 1 NC_008702.1 Conserved hypothetical phenylacetyl-CoA:acceptor oxidoreductase. Homology to padB of Azoarcus sp. EbN1 of 58% (gnl|keqq|eba:ebA5393(KEGG)). InterPro: Prokaryotic molybdopterin oxidoreductases (IPR006655). Pfam: Molypdopterin oxidoreductase; Molydopterin dinucleotide binding domain. no signal peptide. no TMHs; Function unclear; phenylacetyl-CoA:acceptor oxidoreductase 2139191 padB 4607141 padB Azoarcus sp. BH72 phenylacetyl-CoA:acceptor oxidoreductase YP_933450.1 2136465 D 62928 CDS YP_933451.1 119898238 4609488 2139188..2139835 1 NC_008702.1 Conserved hypothetical phenylacetyl-CoA:acceptor oxidoreductase. Homology to padC of A. evansiin of 61% (trembl|Q8L3B5). Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes (By similarity). Pfam: 4Fe-4S binding domain. Tigrfam: napF: ferredoxin-type protein NapF. no signal peptide. no TMHs; Family membership; phenylacetyl-CoA:acceptor oxidoreductase 2139835 padC 4609488 padC Azoarcus sp. BH72 phenylacetyl-CoA:acceptor oxidoreductase YP_933451.1 2139188 D 62928 CDS YP_933452.1 119898239 4609489 2139832..2140698 1 NC_008702.1 Conserved hypothetical phenylacetyl-CoA:acceptor oxidoreductase. Homology to padD of A. evansii of 43% (trembl|Q8L3B4(SRS) Pfam: DMSO reductase anchor subunit (DmsC) The terminal electron transfer enzyme Me2SO reductase of Escherichia coli is a heterotrimeric enzyme composed of a membrane extrinsic catalytic dimer (DmsAB) and a membrane intrinsic polytopic anchor subunit (DmsC). no signal peptide. 8 TMHs; Family membership; phenylacetyl-CoA:acceptor oxidoreductase 2140698 padD 4609489 padD Azoarcus sp. BH72 phenylacetyl-CoA:acceptor oxidoreductase YP_933452.1 2139832 D 62928 CDS YP_933453.1 119898240 4609490 complement(2140807..2141832) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2141832 4609490 azo1949 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933453.1 2140807 R 62928 CDS YP_933454.1 119898241 4607142 2142429..2143481 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03003315 of Dechloromonas aromatica of 48% (gi|53729651|ref|ZP_00348492.1|(NBCI ENTREZ)). InterPro: BNR repeat (IPR002860) Pfam: BNR repeat BNR repeats are short repeats never found closer than 40 residues together, which suggests that the repeat is structurally longer. These repeats are found in many glycosyl hydrolases as well as other extracellular proteins of unknown function. no singal peptide. 1 TMHs; hypothetical protein 2143481 4607142 azo1950 Azoarcus sp. BH72 hypothetical protein YP_933454.1 2142429 D 62928 CDS YP_933455.1 119898242 4607143 2143526..2145898 1 NC_008702.1 Probable Putative membrane protein MJ1562. TREMBL:Q8G9A8:32% identity, 52% similarity InterPro:IPR000873; AMP-bind. IPR002114; HPr_SerP_S Pfam:Patched:Patched family 2A067: efflux transporter putative Signal peptide present (Signal P predicted) TMH's 10 (TMHMM predicted); Family membership; RND superfamily exporter 2145898 4607143 azo1951 Azoarcus sp. BH72 RND superfamily exporter YP_933455.1 2143526 D 62928 CDS YP_933456.1 119898243 4607144 2145954..2147552 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03003313 of Dechloromonas aromatica of 60% (gi|53729649|ref|ZP_00149978.2|(NBCI ENTREZ)). Has PF06980, Protein of unknown function (DUF1302);IPR010727,DUF1302;This family contains a number of hypothetical bacterial proteins of unknown function that are approximately 600 residues long. Most family members seem to be from Pseudomonas. Signal peptide present. No TMHs; Conserved hypothetical protein; hypothetical protein 2147552 4607144 azo1952 Azoarcus sp. BH72 hypothetical protein YP_933456.1 2145954 D 62928 CDS YP_933457.1 119898244 4607145 2147569..2148837 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Raeut03000741 of Ralstonia eutropha of 45% (gi|46131405|ref|ZP_00169583.2|(NBCI ENTREZ)). Pfam: Protein of unknown function (DUF1329). This family consists of several hypothetical bacterial proteins of around 475 residues in length. The majority of family members are from Pseudomonas species but the family also contains sequences from Shewanella oneidensis and Thauera aromatica. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 2148837 4607145 azo1953 Azoarcus sp. BH72 hypothetical protein YP_933457.1 2147569 D 62928 CDS YP_933458.1 119898245 4607146 complement(2149426..2150184) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases. InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 2150184 4607146 azo1954 Azoarcus sp. BH72 short-chain dehydrogenase YP_933458.1 2149426 R 62928 CDS YP_933459.1 119898246 4607147 complement(2150473..2151930) 1 NC_008702.1 catalyzes the formation of indole-3-acetic acid from indole-3-acetamide; indole acetimide hydrolase 2151930 tms 4607147 tms Azoarcus sp. BH72 indole acetimide hydrolase YP_933459.1 2150473 R 62928 CDS YP_933460.1 119898247 4609954 complement(2151974..2153371) 1 NC_008702.1 Putative amidase amiD; Family membership; amidase 2153371 amiD 4609954 amiD Azoarcus sp. BH72 amidase YP_933460.1 2151974 R 62928 CDS YP_933461.1 119898248 4608310 complement(2153368..2154240) 1 NC_008702.1 TREMBLNEW:47357: 69% identity, 80% similarity. 2-hydroxy-6-oxo-6-phenylhexa-24-dienoate hydrolase. The alpha/beta hydrolase fold [1] is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The core of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8 strands connected by helices Pfam: Ndr family. TIGR00148: conserved hypothetical protein; High confidence in function and specificity; 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase 2154240 mhpC 4608310 mhpC Azoarcus sp. BH72 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase YP_933461.1 2153368 R 62928 CDS YP_933462.1 119898249 4609219 complement(2154237..2154995) 1 NC_008702.1 Putative Hydantoin racemase,28% Identity toTrEMBL;Q7NTB3,Q885G0,Q6YNI1. SProt;Q00924. Has PF01177,Asp/Glu/Hydantoin racemase;IPR001920, Asp/Glu_race; This family contains aspartate racemase, glutamate racemase,hydantoin racemase and arylmalonate decarboxylase. Aspartate racemase (EC: 5.1.1.13) and glutamate racemase (EC: 5.1.1.3) are two evolutionary related bacterial enzymes that do not seem to require a cofactor for their activity . Glutamate racemase, which interconverts L-glutamate into D-glutamate, is required for the biosynthesis of peptidoglycan and some peptide-based antibiotics such as gramicidin S. In addition to characterized aspartate and glutamate racemases, this family also includes a hypothetical protein from Erwinia carotovora and one from Escherichia coli (ygeA).Two conserved cysteines are present in the sequence of these enzymes. They are expected to play a role in catalytic activity by acting as bases in proton abstraction from the substrate.; putative Hydantoin racemase 2154995 4609219 azo1958 Azoarcus sp. BH72 putative Hydantoin racemase YP_933462.1 2154237 R 62928 CDS YP_933463.1 119898250 4607148 complement(2155368..2156693) 1 NC_008702.1 Hypothetical protein SignalP reporting Non-secretory protein.; hypothetical protein 2156693 4607148 azo1959 Azoarcus sp. BH72 hypothetical protein YP_933463.1 2155368 R 62928 CDS YP_933464.1 119898251 4607149 complement(2156716..2157738) 1 NC_008702.1 Reductase component OxoR, of the 2-oxo-1,2-dihydroquinoline 8 monooxygenase. Enzyme involved in the second step of quinoline degradation by P. putida 86. Involved in bacterial aromatic compounds degradation.; High confidence in function and specificity; putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase reductase subunit 2157738 oxoR 4607149 oxoR Azoarcus sp. BH72 putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase reductase subunit YP_933464.1 2156716 R 62928 CDS YP_933465.1 119898252 4609463 complement(2157971..2159311) 1 NC_008702.1 Oxygenase component OxoO, of the 2-oxo-1,2-dihydroquinoline 8 monooxygenase. Enzyme involved in the second step of quinoline degradation by P. putida 86. Involved in bacterial aromatic compounds degradation.; Function unclear; putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase oxygenase subunit 2159311 oxoO1 4609463 oxoO1 Azoarcus sp. BH72 putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase oxygenase subunit YP_933465.1 2157971 R 62928 CDS YP_933466.1 119898253 4609461 complement(2159507..2160076) 1 NC_008702.1 Conserved hypothetical flavin reductase. Homology to rsc0763 of R. solanacearum of 43% (TREMBL:Q8Y1C7). Pfam: Flavin reductase like domain. This is a flavin reductase family consisting of enzymes known to be flavin reductases as well as various oxidoreductase and monooxygenase components. no signal peptide. no TMHs; Conserved hypothetical protein; flavin reductase 2160076 4609461 azo1962 Azoarcus sp. BH72 flavin reductase YP_933466.1 2159507 R 62928 CDS YP_933467.1 119898254 4607150 complement(2160073..2161302) 1 NC_008702.1 Conserved Hypothetical protein has strong similarity 70% and 51% identity to PUTATIVE OXYGENASE SUBUNIT PROTEIN [Ralstonia solanacearum GMI1000],TrEMBL:Q8XYC1, which has IPR003042; Rng_mnoxygenase and IPR001327; FAD_pyr_redox domain which is absent in this case. No Signal peptide or TMH being reported as present.; Function unclear; hypothetical protein 2161302 4607150 azo1963 Azoarcus sp. BH72 hypothetical protein YP_933467.1 2160073 R 62928 CDS YP_933468.1 119898255 4607151 complement(2161449..2162435) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2162435 4607151 azo1964 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933468.1 2161449 R 62928 CDS YP_933469.1 119898256 4607152 complement(2162520..2163521) 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2163521 4607152 azo1965 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933469.1 2162520 R 62928 CDS YP_933470.1 119898257 4607153 complement(2163648..2164532) 1 NC_008702.1 Methylcatechol 2,3-dioxygenase, TodE, is involved in the degradation of toluene. Similar to sprot|TODE_PSEPU (38%) and to trembl|Q52031 (36%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily; Specificity unclear; 3-methylcatechol 2,3-dioxygenase 2164532 todE 4607153 todE Azoarcus sp. BH72 3-methylcatechol 2,3-dioxygenase YP_933470.1 2163648 R 62928 CDS YP_933471.1 119898258 4609956 complement(2164557..2165000) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003325 of Dechloromonas aromatica of 52% (gi|53729660|ref|ZP_00348498.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 2165000 4609956 azo1967 Azoarcus sp. BH72 hypothetical protein YP_933471.1 2164557 R 62928 CDS YP_933472.1 119898259 4607154 complement(2165047..2165901) 1 NC_008702.1 TREMBL:Q93JW6: 67% identity, 81% similarity. 2-hydroxymuconic semialdehyde hydrolase (HMSH). hydrolysis of C-C bonds in ketonic substances CONVERSION OF 2-HYDROXYMUCONIC ACID SEMIALDEHYDE TO 2-HYDROXYPENT-24-DIENOATE. Pfam: abhydrolase: alpha/Beta hydrolase fold; thioesterase domain Absence of transmembrane helices (TMHMM predicted) prfB: peptide chain release factor 2; High confidence in function and specificity; 2-hydroxy-muconic semialdehyde hydrolase 2165901 xylF 4607154 xylF Azoarcus sp. BH72 2-hydroxy-muconic semialdehyde hydrolase YP_933472.1 2165047 R 62928 CDS YP_933473.1 119898260 4610134 2166074..2166811 1 NC_008702.1 GntR family transcriptional regulator,; High confidence in function and specificity; GntR family transcriptional regulator 2166811 bphS 4610134 bphS Azoarcus sp. BH72 GntR family transcriptional regulator YP_933473.1 2166074 D 62928 CDS YP_933474.1 119898261 4608403 complement(2166833..2167366) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Rhizobium sp. NGR234 of 50% (tremblnew|AAQ87234(SRS)). No domain predicted. No signal peptide or TMH reported present.; hypothetical protein 2167366 4608403 azo1970 Azoarcus sp. BH72 hypothetical protein YP_933474.1 2166833 R 62928 CDS YP_933475.1 119898262 4607155 complement(2167420..2168526) 1 NC_008702.1 SPROT:ADH1_BACST:31% identity; 45% similarity Alcohol dehydrogenase (EC 1.1.1.1) (ADH-T). THERMOSTABLE NAD(+)-DEPENDENT ALCOHOL DEHYDROGENASE. an alcohol + nad(+) = an aldehyde or ketone + nadh. cofactor:binds 2 zinc ions per subunit (by similarity). InterPro: IPR002328; ADH_zinc. IPR002085:Adh_zn_family. Pfam:PF00107; ADH_zinc_N tdh: L-threonine 3-dehydrogenase; Specificity unclear; putative alcohol dehydrogenase 2168526 4607155 azo1971 Azoarcus sp. BH72 putative alcohol dehydrogenase YP_933475.1 2167420 R 62928 CDS YP_933476.1 119898263 4607156 complement(2168585..2169613) 1 NC_008702.1 catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase 2169613 mhpE 4607156 mhpE Azoarcus sp. BH72 4-hydroxy-2-ketovalerate aldolase YP_933476.1 2168585 R 62928 CDS YP_933477.1 119898264 4609221 complement(2169628..2170548) 1 NC_008702.1 catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase 2170548 mhpF 4609221 mhpF Azoarcus sp. BH72 acetaldehyde dehydrogenase YP_933477.1 2169628 R 62928 CDS YP_933478.1 119898265 4609222 complement(2170617..2171411) 1 NC_008702.1 This family consist of various hydratases and 4-oxalocrotonate decarboxylases which are involved in the bacterial meta-cleavage pathways for degradation of aromatic compounds, TREMBL:Q51981 (54% identity); SWISSPROT:P77608 (50% identity). InterPro (IPR002607): Hydratase/decarboxylase Pfam (PF01689): Hydratase/decarboxylase.; High confidence in function and specificity; MhpD protein 2171411 mhpD 4609222 mhpD Azoarcus sp. BH72 MhpD protein YP_933478.1 2170617 R 62928 CDS YP_933479.1 119898266 4609220 complement(2171448..2172401) 1 NC_008702.1 catalyzes the cleavage of 3-(2,3-dihydroxyphenyl) propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate; part of the 3-phenylpropionic acid degradation pathway; member of the protocatechuate 4,5-dioxygenase family; 3-(2,3-dihydroxyphenyl)propionate dioxygenase 2172401 mhpB 4609220 mhpB Azoarcus sp. BH72 3-(2,3-dihydroxyphenyl)propionate dioxygenase YP_933479.1 2171448 R 62928 CDS YP_933480.1 119898267 4609218 complement(2172426..2173322) 1 NC_008702.1 Hypothetical protein Rv2715/MT2788/Mb2734. TREMBL:Q9KH20: 58% identity, 73% similarity 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase; OhpC [Rhodococcus sp.] Catalysis of the reaction: 2-hydroxy-6-keto-nona-2,4-dienedioate + OH- = cis-2-hydroxypenta-2,4-dienoate + succinate. Pfam: alpha/ beta hydrolase; Ndr family; B12 binding domain. No signal peptide (Signal P predicted) or transmembrane helices (TMHMM predited) TIGRFAM: Co A. E activ TIGR00044: conserved hypothetical protein; Family membership; 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase 2173322 ohpC 4609218 ohpC Azoarcus sp. BH72 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase YP_933480.1 2172426 R 62928 CDS YP_933481.1 119898268 4609439 complement(2173724..2175064) 1 NC_008702.1 Oxygenase component OxoO, of the 2-oxo-1,2-dihydroquinoline 8 monooxygenase. Enzyme involved in the second step of quinoline degradation by P. putida 86. Involved in bacterial aromatic compounds degradation. InterPro: Rieske iron-sulfur protein 2Fe-2S subunit arcC: carbamate kinase; Function unclear; putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase oxygenase subunit 2175064 oxoO2 4609439 oxoO2 Azoarcus sp. BH72 putative 2-oxo-1 2-dihydroquinoline 8-monooxygenase oxygenase subunit YP_933481.1 2173724 R 62928 CDS YP_933482.1 119898269 4609462 2175414..2176190 1 NC_008702.1 TREMBL:Q89PP9: 66% identity, 79% similarity. InterPro:IPR007325; Cyclase. Pfam:PF04199; Cyclase No signal peptide present. Absence of trans-membrane helices (TMHMM predicted).; Function unclear; hypothetical protein 2176190 4609462 azo1978 Azoarcus sp. BH72 hypothetical protein YP_933482.1 2175414 D 62928 CDS YP_933483.1 119898270 4607157 2176634..2176756 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2176756 4607157 azo1979 Azoarcus sp. BH72 hypothetical protein YP_933483.1 2176634 D 62928 CDS YP_933484.1 119898271 4607158 2176749..2178953 1 NC_008702.1 Conserved hypothetical indolepyruvate oxidoreductase, alpha subunit. Homology to mlr5448 of M. loti of 69% (trembl|Q98BS4). CATALYZES THE FERREDOXIN-DEPENDENT OXIDATIVE DECARBOXYLATION OF ARYLPYRUVATES (BY SIMILARITY). no signal peptide. no TMHs; Conserved hypothetical protein; indolepyruvate ferredoxin oxidoreductase subunit alpha 2178953 4607158 azo1980 Azoarcus sp. BH72 indolepyruvate ferredoxin oxidoreductase subunit alpha YP_933484.1 2176749 D 62928 CDS YP_933485.1 119898272 4607159 2178974..2180560 1 NC_008702.1 Involved in the incorporation of exogenous aryl acids in the biosynthesis of aromatic amino acids: catalysis of the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.; putative indolepyruvate oxidoreductase subunit B 2180560 4607159 azo1981 Azoarcus sp. BH72 putative indolepyruvate oxidoreductase subunit B YP_933485.1 2178974 D 62928 CDS YP_933486.1 119898273 4607160 2180606..2181583 1 NC_008702.1 Probable vanillate O-demethylase oxidoreductase (Vanillate degradation ferredoxin-like protein). Homology to vanB of P. sp HR199 of 42% (sprot|VANB_PSEUH). The vanillate demethylase (EC:1.14.13.82) consists of two proteins: an oxygenase and an oxygenase reductase (VanA and VanB). This enzyme is involved in the vanillate degradation, a key intermediate in the degradation of lignin. Pfam: Oxidoredutase FAD-binding domain; Oxidoreductase NAD-binding domain; 2Fe-2S iron-sulfur cluster binding domain no signal peptide no TMHs; Family membership; vanillate O-demethylase oxidoreductase 2181583 vanB2 4607160 vanB2 Azoarcus sp. BH72 vanillate O-demethylase oxidoreductase YP_933486.1 2180606 D 62928 CDS YP_933487.1 119898274 4610091 2181600..2182922 1 NC_008702.1 Hydroxylase large component of 1,2-dioxygenase complex, involved in aromatic compounds degradation. Putative 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase, CarBb. Involved in the aerobic degradation of carbazole by P.resinovorans sp. strain CA10. XlyY: Benzoate 12-dioxygenase beta subunit (EC 1.14.12.10). DEGRADATION OF BENZOATE TO 2-HYDRO-12-DIHYDROXYBENZOATE (DHB).; High confidence in function and specificity; putative dioxygenase hydroxylase large subunit 2182922 carBb2 4610091 carBb2 Azoarcus sp. BH72 putative dioxygenase hydroxylase large subunit YP_933487.1 2181600 D 62928 CDS YP_933488.1 119898275 4608419 2183143..2183625 1 NC_008702.1 Hydroxylase large component of 1,2-dioxygenase complex, involved in aromatic compounds degradation. Putative 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase, CarBa. Involved in the aerobic degradation of carbazole by P.resinovorans sp. strain CA10. XlyX: Benzoate 12-dioxygenase alpha subunit (EC 1.14.12.10). DEGRADATION OF BENZOATE TO 2-HYDRO-12-DIHYDROXYBENZOATE (DHB). Benzoate 12-dioxygenase beta subunit (EC 1.14.12.10). DEGRADATION OF BENZOATE TO 2-HYDRO-12-DIHYDROXYBENZOATE (DHB). THE BETA SUBUNIT MAY BE RESPONSIBLE FOR THE SUBSTRATE SPECIFICITY OF THE ENZYME. InterPro: Aromatic-ring-hydroxylating dioxygenase beta subunit; High confidence in function and specificity; putative dioxygenase, hydroxylase small subunit 2183625 carBa2 4608419 carBa2 Azoarcus sp. BH72 putative dioxygenase, hydroxylase small subunit YP_933488.1 2183143 D 62928 CDS YP_933489.1 119898276 4608417 2183639..2185273 1 NC_008702.1 Similar to TREMBL:Q89PS8 (59% identity). InterPro (IPR000873): AMP-dependent synthetase and ligase. Pfam (PF00501): AMP-binding enzyme.; Function unclear; putative AMP-binding protein 2185273 4608417 azo1985 Azoarcus sp. BH72 putative AMP-binding protein YP_933489.1 2183639 D 62928 CDS YP_933490.1 119898277 4607161 2185270..2186448 1 NC_008702.1 Entry name TREMBL:Q89PQ1 Prim. accession # Q89PQ1 Identities = 241/381 (63%) Thiolase, N-terminal domain (Pfam predicted) Prediction: Non-secretory protein Signal peptide probability: 0.00 Number of predicted TMHs: 0; hypothetical protein 2186448 4607161 azo1986 Azoarcus sp. BH72 hypothetical protein YP_933490.1 2185270 D 62928 CDS YP_933491.1 119898278 4607162 2186445..2187704 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,; Specificity unclear; short-chain dehydrogenase 2187704 4607162 azo1987 Azoarcus sp. BH72 short-chain dehydrogenase YP_933491.1 2186445 D 62928 CDS YP_933492.1 119898279 4607163 2187853..2188638 1 NC_008702.1 TREMBL:Q8XYC3: 64% identity, 75% similarity Proteins in this family are thought to be cyclase enzymes. They are found in proteins involved in antibiotic synthesis. However they are also found in organisms that do not make antibiotics pointing to a wider role for these proteins. The proteins contain a conserved motif HXGTHXDXPXH that is likely to form a part of the active site. InterPro:IPR007325; Cyclase. Pfam: PF04199; Cyclase Absence of signal peptide. Absence of transmembrane helices; Function unclear; hypothetical protein 2188638 4607163 azo1988 Azoarcus sp. BH72 hypothetical protein YP_933492.1 2187853 D 62928 CDS YP_933493.1 119898280 4607164 2188649..2189377 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q7WG84 (46% identity); SWISSPROT:P17611 (30% identity). Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 2189377 4607164 azo1989 Azoarcus sp. BH72 short-chain dehydrogenase YP_933493.1 2188649 D 62928 CDS YP_933494.1 119898281 4607165 2189440..2190591 1 NC_008702.1 The transport of branched-chain amino acids in P. aeruginosa is mediated by two distinct systems, the leucine, isoleucine, and valine I and II transports systems. Periplasmic binding proteins are found to participate in the transport of amino acids, sugars, and ions. BraC is the structural gene for periplasmic binding proteins in P. aeruginosa. Similar to trembl|Q9RYP6 (49%) and to sprot|BRAC_PSEAE (22%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative Leu/Ile/Val-binding protein 2190591 braC 4607165 braC Azoarcus sp. BH72 putative Leu/Ile/Val-binding protein YP_933494.1 2189440 D 62928 CDS YP_933495.1 119898282 4608404 2190910..2191947 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q8XRX6 (55%) and to trembl|Q7WCY3 (53%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting nine Tmhelix.; Specificity unclear; ABC transporter permease 2191947 4608404 azo1991 Azoarcus sp. BH72 ABC transporter permease YP_933495.1 2190910 D 62928 CDS YP_933496.1 119898283 4607166 2191944..2193803 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. The integral inner-membrane proteins translocate the substrate across the membrane. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XRX5 (52%) and to trembl|Q89CG4 (45%). Pfam (PF00005): ABC transporter Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) Smart (SM00382): AAA ATPase superfamily ProSite (PS50101): ATP/GTP-binding site motif A (P-loop) TMHMM reporting nine Tmhelix.; Specificity unclear; ABC transporter ATP-binding protein/permease 2193803 4607166 azo1992 Azoarcus sp. BH72 ABC transporter ATP-binding protein/permease YP_933496.1 2191944 D 62928 CDS YP_933497.1 119898284 4607167 2193803..2194564 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XRX4 (66%) and to trembl|Q89PQ7 (62%). Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 2194564 4607167 azo1993 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_933497.1 2193803 D 62928 CDS YP_933498.1 119898285 4607168 2195039..2196049 1 NC_008702.1 Putative 2-dehydropantoate 2-reductase (EC 1.1.1.169) (KPA reductase) (KPR). Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid (By similarity). apbA_panE: 2-dehydropantoate 2-reductase; Specificity unclear; 2-dehydropantoate 2-reductase 2196049 apbA2 4607168 apbA2 Azoarcus sp. BH72 2-dehydropantoate 2-reductase YP_933498.1 2195039 D 62928 CDS YP_933499.1 119898286 4608326 2196116..2196889 1 NC_008702.1 41% Aldolase_II_N. Class II Aldolase and Adducin N-terminal domain Pfam: PF00596; Aldolase_II; 1. fucA: L-fuculose phosphate aldolase; High confidence in function and specificity; hypothetical protein 2196889 4608326 azo1995 Azoarcus sp. BH72 hypothetical protein YP_933499.1 2196116 D 62928 CDS YP_933500.1 119898287 4607169 2197112..2198719 1 NC_008702.1 Acetyl-coenzyme A synthetase enables the cell to use acetate during aerobic growth to generate energy via the tca cycle, and biosynthetic compounds via the glyoxylate shunt. acetylates chey, the response regulator involved in flagellar movement and chemotaxis. Entry name SWISSPROT:ACSA_ECOLI Prim. accession # P27550 Identities = 145/546 (26%) Pfam PF00501; AMP-binding; 1. InterPro IPR000873; AMP-bind. Pfam PF00501; AMP-binding; 1. Number of predicted TMHs: 0; Family membership; putative acetyl-coenzyme a synthetase 2198719 acS1 4607169 acS1 Azoarcus sp. BH72 putative acetyl-coenzyme a synthetase YP_933500.1 2197112 D 62928 CDS YP_933501.1 119898288 4608272 2198864..2199388 1 NC_008702.1 Putative transcriptional activatory protein BadR (Benzoate anaerobic degradation regulator). Similar to SWISSPROT: sprot|BADR_RHOPA (25% Rhodopseudomonas palustris, transcriptional activatory protein BadR (benzoate anaerobic degradation regulator)) InterPro: IPR000835 HTH_MarR. Pfam: PF01047 MarR family.; Family membership; MarR family transcriptional regulator 2199388 badR 4608272 badR Azoarcus sp. BH72 MarR family transcriptional regulator YP_933501.1 2198864 D 62928 CDS YP_933502.1 119898289 4608374 complement(2199402..2200142) 1 NC_008702.1 Catalyzes the phosphorolytic cleavage of 6-oxopurine nucleosides; 5'-methylthioadenosine phosphorylase 2200142 4608374 azo1998 Azoarcus sp. BH72 5'-methylthioadenosine phosphorylase YP_933502.1 2199402 R 62928 CDS YP_933503.1 119898290 4607170 2200267..2202084 1 NC_008702.1 GTP-binding elongation factor,; High confidence in function and specificity; GTP-binding elongation factor 2202084 typA 4607170 typA Azoarcus sp. BH72 GTP-binding elongation factor YP_933503.1 2200267 D 62928 CDS YP_933504.1 119898291 4610053 complement(2202353..2203258) 1 NC_008702.1 UTP--glucose-1-phosphate uridylyltransferase 1 (EC 2.7.7.9) (UDP- glucose pyrophosphorylase 1) (UDPGP 1) (Alpha-D-glucosyl-1-phosphate uridylyltransferase 1) (Uridine diphosphoglucose pyrophosphorylase 1). InterPro: ADP-glucose pyrophosphorylase ispD: 4-diphosphocytidyl-2C-methyl-D-er; High confidence in function and specificity; UTP-glucose-1-phosphate uridylyltransferase 2203258 galU 4610053 galU Azoarcus sp. BH72 UTP-glucose-1-phosphate uridylyltransferase YP_933504.1 2202353 R 62928 CDS YP_933505.1 119898292 4608857 complement(2203255..2205336) 1 NC_008702.1 DNA ligase (Polydeoxyribonucleotide synthase [NAD+]). This protein catalyzes the formation of phosphodiester linkages between 5-phosphoryl and 3-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. TIGRFAM: dnlj: DNA ligase NAD-dependent.; High confidence in function and specificity; DNA ligase 2205336 4608857 azo2001 Azoarcus sp. BH72 DNA ligase YP_933505.1 2203255 R 62928 CDS YP_933506.1 119898293 4607171 complement(2205341..2206462) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ne2283 of N. europaea of 35% (trembl|Q82SM8(SRS)). Pfam: ZipA, C-ternial FtsZ-binding domaine. This family represents the ZipA C-terminal domain. ZipA is involved in septum formation in bacterial cell division. Its C-terminal domain binds FtsZ, a major component of the bacterial septal ring. The structure of this domain is an alpha-beta fold with three alpha helices and a beta sheet of six antiparallel beta strands. The major loops protruding from the beta sheet surface are thought to form a binding site for FtsZ. no singal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2206462 4607171 azo2002 Azoarcus sp. BH72 hypothetical protein YP_933506.1 2205341 R 62928 CDS YP_933507.1 119898294 4607172 complement(2206478..2210014) 1 NC_008702.1 Chromosome partition protein smc. PLAYS AN IMPORTANT ROLE IN CHROMOSOME STRUCTURE AND PARTITIONING. ESSENTIAL FOR CHROMOSOME PARTITION. InterPro: SMC family C-terminal domain; High confidence in function and specificity; chromosome segregation protein 2210014 smc 4607172 smc Azoarcus sp. BH72 chromosome segregation protein YP_933507.1 2206478 R 62928 CDS YP_933508.1 119898295 4609876 2210146..2211420 1 NC_008702.1 catalyzes the formation of succinyldiaminopimelate from N-succinyl-2-amino-6-ketopimelate; succinyldiaminopimelate transaminase 2211420 dapC 4609876 dapC Azoarcus sp. BH72 succinyldiaminopimelate transaminase YP_933508.1 2210146 D 62928 CDS YP_933509.1 119898296 4608582 2211431..2212252 1 NC_008702.1 catalyzes the formation of N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and tetrahydrodipicolinate in the lysine biosynthetic pathway; 2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase 2212252 dapD 4608582 dapD Azoarcus sp. BH72 2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase YP_933509.1 2211431 D 62928 CDS YP_933510.1 119898297 4608583 2212337..2213524 1 NC_008702.1 Tfp pilus retraction protein pilU, probable involved in twitching motility mechanism,; Specificity unclear; twitching motility protein 2213524 4608583 azo2006 Azoarcus sp. BH72 twitching motility protein YP_933510.1 2212337 D 62928 CDS YP_933511.1 119898298 4607173 2213566..2214708 1 NC_008702.1 dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE), catalyzes the hydrolysis of N-succinyl-L,Ldiaminopimelate L,L-SDAP to L,L-diaminopimelate and succinate. It is a metalloprotease containing dinuclear active sites. Its structure is similar to the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 and the aminopeptidase from Aeromonas proteolytica.; succinyl-diaminopimelate desuccinylase 2214708 dapE 4607173 dapE Azoarcus sp. BH72 succinyl-diaminopimelate desuccinylase YP_933511.1 2213566 D 62928 CDS YP_933512.1 119898299 4608584 2214717..2215679 1 NC_008702.1 involved in methylation of ribosomal protein L3; N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 2215679 4608584 azo2008 Azoarcus sp. BH72 N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase YP_933512.1 2214717 D 62928 CDS YP_933513.1 119898300 4607174 2215747..2216205 1 NC_008702.1 Putative Response regulator rcp1. Forms a two-component system with cph1 in which it acts as reciever substrate. Similar to SWISSPROT: sprot|RCP1_SYNY3 (30% Synechocystis sp., Rcp1) Pfam: PF00072 Response_reg.; Function unclear; putative two-component response regulator 2216205 rcp1 4607174 rcp1 Azoarcus sp. BH72 putative two-component response regulator YP_933513.1 2215747 D 62928 CDS YP_933514.1 119898301 4609696 complement(2216245..2216439) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV1087 of C.violaceum of 69% (trembl:Q7NZ35). Has PF04384 ; Protein of unknown function (DUF528);IPR007479: Small bacterial protein of unknown function. No signal peptide or TMH present.; hypothetical protein 2216439 4609696 azo2010 Azoarcus sp. BH72 hypothetical protein YP_933514.1 2216245 R 62928 CDS YP_933515.1 119898302 4607175 complement(2216436..2216780) 1 NC_008702.1 Ferredoxin 2Fe-2S. Homology to fdx of E. coli of 70% (sprot|FER_ECOLI ). Ferredoxin are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Although the function of this ferredoxin is unknown it is probable that it has a role as a cellular electron transfer protein. Involved in the in vivo assembly of the Fe-S clusters in a wide variety of iron- sulfur proteins. InterPro: Ferredoxin (IPR001055); Adrenodoxin (IPR001055) Pfam: 2Fe-2S iorn-sulfur cluster binding domain no signal peptide no TMHs; High confidence in function and specificity; Fdx2 protein 2216780 fdx2 4607175 fdx2 Azoarcus sp. BH72 Fdx2 protein YP_933515.1 2216436 R 62928 CDS YP_933516.1 119898303 4608746 complement(2216777..2218645) 1 NC_008702.1 involved in the maturation of iron-sulfur cluster-containing proteins; chaperone protein HscA 2218645 hscA 4608746 hscA Azoarcus sp. BH72 chaperone protein HscA YP_933516.1 2216777 R 62928 CDS YP_933517.1 119898304 4607176 complement(2218661..2219194) 1 NC_008702.1 J-type co-chaperone that regulates the ATPase and peptide-binding activity of Hsc66 chaperone; may function in biogenesis of iron-sulfur proteins; co-chaperone HscB 2219194 hscB 4607176 hscB Azoarcus sp. BH72 co-chaperone HscB YP_933517.1 2218661 R 62928 CDS YP_933518.1 119898305 4609030 complement(2219199..2219522) 1 NC_008702.1 Putative regulatory Protein yfhF, 72% identity (82% similarity) to TrEMBL;P36539 of E.coli. Has PF01521, HesB-like domain;(IPR000361, HesB_yadR_yfhF); This family includes HesB which may be involved in nitrogen fixation; the hesB gene is expressed only under nitrogen fixation conditions. Other members of this family include various hypothetical proteins of which P46847 and NP31774 also contains NifU-like domains NifU which is also involved in nitrogen fixation. In the gram-negative soil bacterium Rhizobium etli, the hesB-like gene iscN is required for nitrogen fixation.; High confidence in function and specificity; putative regulatory protein 2219522 yfhF 4609030 yfhF Azoarcus sp. BH72 putative regulatory protein YP_933518.1 2219199 R 62928 CDS YP_933519.1 119898306 4610173 complement(2219543..2219926) 1 NC_008702.1 NifU-related protein. Homology to iscU of A. vinelandii of 79% (trembl|O31270). IscU is a homologue of the N-terminal region of NifU, an protein MAY BE INVOLVED IN THE FORMATION OR REPAIR OF [FE-S] CLUSTERS PRESENT IN IRON-SULFUR PROTEINS mostly found in nitrogen-fixing bacteria. InterPro: NifU-like N terminal domain (IPR002871) Pfam: NifU-like N-terminal domain no signal peptide no TMHs; High confidence in function and specificity; scaffold protein 2219926 iscU 4610173 iscU Azoarcus sp. BH72 scaffold protein YP_933519.1 2219543 R 62928 CDS YP_933520.1 119898307 4609091 complement(2219958..2221169) 1 NC_008702.1 Cysteine desulfurase (NifS protein homolog). Homology to iscS of E. coli of 72% (sprot|ISCS_ECOLI). Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). Functions as a sulfur delivery protein for nad, biotin and fe-s cluster synthesis. Pfam: Aminotransferase class V no signal peptide no TMHs; High confidence in function and specificity; cysteine desulfurase 2221169 iscS1 4609091 iscS1 Azoarcus sp. BH72 cysteine desulfurase YP_933520.1 2219958 R 62928 CDS YP_933521.1 119898308 4609089 complement(2221203..2222351) 1 NC_008702.1 Probable cysteine desulfurase (NifS protein homolog). Homology to iscS of M. thermophila of 40% (sprot|ISCS_METTE) Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). InterPro: Aminotransferase class-V (IPR000192) Pfam: aminotransferase class-V no signal peptide no TMHs ahpD: alkylhydroperoxidase AhpD; Family membership; cysteine desulfurase 2222351 iscS2 4609089 iscS2 Azoarcus sp. BH72 cysteine desulfurase YP_933521.1 2221203 R 62928 CDS YP_933522.1 119898309 4609090 complement(2222358..2222840) 1 NC_008702.1 RrF2 family protein. These are small proteins of 12 to 18 kD which seem to contain a signal sequence, and may represent a family of probable transcriptional regulators. Similar to SWISSPROT: sprot|YOR2_AZOVI (50% Azotobacter vinelandii, hypothetical upf0074 protein) / TREMBL: trembl|Q8Y0M5 (72% Ralstonia solanacearum (Pseudomonas solanacearum), hypothetical protein rsc1018) Pfam: PF02082 Transcriptional regulator. TIGRFAM: TIGR00738 rrf2 family protein (Uncharacterized protein family UPF0074 / putative transcriptional regulator). Signal P reporting signal peptide. HTH reporting nucleic acid binding motif.; Family membership; Rrf2 family protein 2222840 4609090 azo2018 Azoarcus sp. BH72 Rrf2 family protein YP_933522.1 2222358 R 62928 CDS YP_933523.1 119898310 4607177 complement(2222961..2223716) 1 NC_008702.1 Probable serine acetyltransferase (EC 2.3.1.30). Homology to cysE of B. subtilis of 48% (sprot|CYSE_BACSU). no signal peptide no TMHs; Family membership; serine O-acetyltransferase 2223716 cysE 4607177 cysE Azoarcus sp. BH72 serine O-acetyltransferase YP_933523.1 2222961 R 62928 CDS YP_933524.1 119898311 4608557 complement(2223803..2224531) 1 NC_008702.1 tRNA/rRNA methyltransferase; Specificity unclear; RNA methyltransferase 2224531 4608557 azo2020 Azoarcus sp. BH72 RNA methyltransferase YP_933524.1 2223803 R 62928 CDS YP_933525.1 119898312 4607178 2224621..2225433 1 NC_008702.1 Inositol-1-monophosphatase (EC 3.1.3.25) (IMPase) (Inositol-1- phosphatase) (I-1-Pase).May act by enhancing the synthesis or degradation of phosphorylated messenger molecules. Catalytic activity: myo-inositol 1-phosphate + h(2)o = myo- inositol + phosphate. 52% Inositol_P. Pfam:PF00459; inositol_P; 1.; High confidence in function and specificity; putative inositol-1-monophosphatase 2225433 suhB1 4607178 suhB1 Azoarcus sp. BH72 putative inositol-1-monophosphatase YP_933525.1 2224621 D 62928 CDS YP_933526.1 119898313 4609906 complement(2225579..2226112) 1 NC_008702.1 Conserved hypotheticalsecreted protein. Homology to bll4707 of B.japonicum of 40% (tremble:Q89L41). No domains predicted. Signal P reporting signal peptide present. No TMH reported to be present.; Conserved hypothetical protein; hypothetical protein 2226112 4609906 azo2022 Azoarcus sp. BH72 hypothetical protein YP_933526.1 2225579 R 62928 CDS YP_933527.1 119898314 4607179 2227005..2227280 1 NC_008702.1 Family membership; hypothetical protein 2227280 4607179 azo2023 Azoarcus sp. BH72 hypothetical protein YP_933527.1 2227005 D 62928 CDS YP_933528.1 119898315 4607180 2227270..2227524 1 NC_008702.1 Conserved Hypothetical protein,[yoeB],69% identical(85% similarity) to SwissPort;P56605. TrEMBL;Q8FG53 Has PF05015, Plasmid maintenance system killer protein (IPR007711, Plasmid_killer) ;Several plasmids with proteic killer gene systems have been reported. All of them encode a stable toxin and an unstable antidote. Upon loss of the plasmid, the less stable inhibitor is inactivated more rapidly than the toxin, allowing the toxin to be activated. The activation of those systems result in cell filamentation and cessation of viable cell production. It has been verified that both the stable killer and the unstable inhibitor of the systems are short polypeptides. This family corresponds to the toxin. Has (IPR009614)PF06769, Protein of unknown function (DUF1224);This family consists of several short, hypothetical bacterial proteins of around 85 residues in length. The function of this family is unknown.; hypothetical protein 2227524 yoeB 4607180 yoeB Azoarcus sp. BH72 hypothetical protein YP_933528.1 2227270 D 62928 CDS YP_933529.1 119898316 4610195 complement(2227858..2228355) 1 NC_008702.1 Conserved hypothetical acetyltransferase. Homology to glr3475 of G. violaceus of 40% (trembl|Q7NFP9). Pfam: Acetyltransferase (GNAT) family. no signal peptide. no TMHs; Family membership; acetyltransferase 2228355 4610195 azo2025 Azoarcus sp. BH72 acetyltransferase YP_933529.1 2227858 R 62928 CDS YP_933530.1 119898317 4607181 complement(2228352..2228645) 1 NC_008702.1 Conserved hypothetical protein. Homology to plu0180 of P.luminescens of 37% (trembl:Q7N9X1). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2228645 4607181 azo2026 Azoarcus sp. BH72 hypothetical protein YP_933530.1 2228352 R 62928 CDS YP_933531.1 119898318 4607182 2229090..2229269 1 NC_008702.1 Conserved hypothetical truncated transposase. Homology to Reut02001235 of Ralstonia metallidurans of 31% (gi|48772472|ref|ZP_00276814.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; Conserved hypothetical protein; putative transposase 2229269 4607182 azo2027 Azoarcus sp. BH72 putative transposase YP_933531.1 2229090 D 62928 CDS YP_933532.1 119898319 4607183 2229309..2229731 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology of the entire protein to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 2229731 4607183 azo2028 Azoarcus sp. BH72 hypothetical protein YP_933532.1 2229309 D 62928 CDS YP_933533.1 119898320 4607184 2229758..2230090 1 NC_008702.1 Conserved hypothetical transposase. Homology to ebB8 of Azoarcus sp. EbN1 of 91% (gnl|keqq|eba:ebB8(KEGG)). Pfam: IS66 Orf2 like protein. This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition. No signal peptide. Probable 1 TMHs; Conserved hypothetical protein; putative transposase 2230090 4607184 azo2029 Azoarcus sp. BH72 putative transposase YP_933533.1 2229758 D 62928 CDS YP_933534.1 119898321 4607185 2230168..2230368 1 NC_008702.1 Hypothetical truncated transposase. Homology to the N-terminus of ebA290 of Azoarcus sp. EbN1 of 92% (gnl|keqq|eba:ebA290(KEGG)). No domains predicted. No signal peptide. No TMHs.; putative transposase 2230368 4607185 azo2030 Azoarcus sp. BH72 putative transposase YP_933534.1 2230168 D 62928 CDS YP_933535.1 119898322 4607186 2230396..2231724 1 NC_008702.1 Hypothetical truncated transposase. Homology to the C-terminus of ebA290 of Azoarcus sp. EbN1 of 67% (gnl|keqq|eba:ebA290(KEGG)). Pfam: Transposase IS66 family. No signal peptide. No TMHs; putative transposase 2231724 4607186 azo2031 Azoarcus sp. BH72 putative transposase YP_933535.1 2230396 D 62928 CDS YP_933536.1 119898323 4607187 2231721..2232221 1 NC_008702.1 Hypothetical protein, very weak homology with hits in the database. Has No domains, repeats, motifs or features detected.; hypothetical protein 2232221 4607187 azo2032 Azoarcus sp. BH72 hypothetical protein YP_933536.1 2231721 D 62928 CDS YP_933537.1 119898324 4607188 complement(2232140..2232754) 1 NC_008702.1 Hypothetical truncated transposase. Homology to the N-terminus of tnp16A of Azoarcus sp. EbN1 of 54% (gnl|keqq|eba:ebA690(KEGG)). No domains predicted. No TMHs. No signal peptide.; putative transposase 2232754 4607188 azo2033 Azoarcus sp. BH72 putative transposase YP_933537.1 2232140 R 62928 CDS YP_933538.1 119898325 4607189 complement(2232808..2233167) 1 NC_008702.1 Conserved hypothetical transposase helper protein. Homology to ebB98 of Azoarcus sp. EbN1 of 74% (gnl|keqq|eba:ebB98(KEGG)). Pfam: IS66 Orf2 like protein. This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition. No signal peptide. No TMHs.; Conserved hypothetical protein; putative transposase helper protein 2233167 4607189 azo2034 Azoarcus sp. BH72 putative transposase helper protein YP_933538.1 2232808 R 62928 CDS YP_933539.1 119898326 4607190 complement(2233167..2233475) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD29 of Azoarcus sp. EbN1 of 55% (gnl|keqq|eba:ebD29(KEGG)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 2233475 4607190 azo2035 Azoarcus sp. BH72 hypothetical protein YP_933539.1 2233167 R 62928 CDS YP_933540.1 119898327 4607191 complement(2234650..2235642) 1 NC_008702.1 hypothetical protein 2235642 4607191 azo2036 Azoarcus sp. BH72 hypothetical protein YP_933540.1 2234650 R 62928 CDS YP_933541.1 119898328 4607192 complement(2236135..2236755) 1 NC_008702.1 Conserved hypothetical protein. Homology to XAC2237 of X.axonopodis of 33% (trembl:Q8PKD8). No domain predicted. No TMHs. No signal peptide.; hypothetical protein 2236755 4607192 azo2037 Azoarcus sp. BH72 hypothetical protein YP_933541.1 2236135 R 62928 CDS YP_933542.1 119898329 4607193 complement(2237096..2237575) 1 NC_008702.1 Conserved hypothetical protein. Homology to RL074 of Pseudomonas aeruginosa of 34% (trembl:Q7WXY6). No domains predicted. No TMHs. No singal peptide.; hypothetical protein 2237575 4607193 azo2038 Azoarcus sp. BH72 hypothetical protein YP_933542.1 2237096 R 62928 CDS YP_933543.1 119898330 4607194 2237967..2238542 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Gritica. No homology to the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 2238542 4607194 azo2039 Azoarcus sp. BH72 hypothetical protein YP_933543.1 2237967 D 62928 CDS YP_933544.1 119898331 4607195 2238649..2239074 1 NC_008702.1 Probable replicase,functional similarity(67%) to TrEMBL Q6I6A6,PF04796;IPR006881 Has pfam04796, RepA_C domain, Plasmid encoded RepA protein. Family of plasmid encoded proteins involved in plasmid replication. The role of RepA in the replication process is not clearly understood. No Signal peptide or TMH are reported to be present.; Specificity unclear; replicase RepA 2239074 repA 4607195 repA Azoarcus sp. BH72 replicase RepA YP_933544.1 2238649 D 62928 CDS YP_933545.1 119898332 4609714 2239144..2239623 1 NC_008702.1 Probable replicase(repA),functional similarity(67%) to TrEMBL Q6I6A6,PF04796;IPR006881 Has pfam04796, RepA_C domain, Plasmid encoded RepA protein. Family of plasmid encoded proteins involved in plasmid replication. The role of RepA in the replication process is not clearly understood. No Signal peptide or TMH are reported to be present.; protein kinase 2239623 4609714 azo2041 Azoarcus sp. BH72 protein kinase YP_933545.1 2239144 D 62928 CDS YP_933546.1 119898333 4607196 2239863..2240681 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA436 of Azoarcus sp. EbN1 of 49% (gnl|keqq|eba:ebA436(KEGG)). No domains predicted. No signal peptide. TMHMM2 reporting presence of 2 TMH's.; Conserved hypothetical protein; hypothetical protein 2240681 4607196 azo2042 Azoarcus sp. BH72 hypothetical protein YP_933546.1 2239863 D 62928 CDS YP_933547.1 119898334 4607197 2240702..2241337 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA439 of Azoarcus sp. EbN1 of 59% (gnl|keqq|eba:ebA439(KEGG)). No domains predicted. No TMHs signal peptide present.; Conserved hypothetical protein; hypothetical protein 2241337 4607197 azo2043 Azoarcus sp. BH72 hypothetical protein YP_933547.1 2240702 D 62928 CDS YP_933548.1 119898335 4607198 2241354..2241563 1 NC_008702.1 Conserved hypothetical protein. No homology to a protein of similar size in the data base. No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2241563 4607198 azo2044 Azoarcus sp. BH72 hypothetical protein YP_933548.1 2241354 D 62928 CDS YP_933549.1 119898336 4607199 2242277..2244052 1 NC_008702.1 Hypothetical protein, 28% identity (45% similarity) to TrEMBL;Q7VHD7. Weak homology with hits in the Database spanning the entire lenghth of Protein. Has PF03235,Protein of unknown function DUF262; Interpro;IPR004919.; Function unclear; hypothetical protein 2244052 4607199 azo2045 Azoarcus sp. BH72 hypothetical protein YP_933549.1 2242277 D 62928 CDS YP_933550.1 119898337 4607200 2244049..2246235 1 NC_008702.1 Hypothetical protein 27% identity to TrEMBL;Q7VHD8. Signal Peptide, or any known features not present.; hypothetical protein 2246235 4607200 azo2046 Azoarcus sp. BH72 hypothetical protein YP_933550.1 2244049 D 62928 CDS YP_933551.1 119898338 4607201 2246568..2247158 1 NC_008702.1 DNA repair protein radC homolog.; High confidence in function and specificity; DNA repair protein 2247158 radC2 4607201 radC2 Azoarcus sp. BH72 DNA repair protein YP_933551.1 2246568 D 62928 CDS YP_933552.1 119898339 4609692 2247594..2248190 1 NC_008702.1 Hypothetical protein yfdR. The HD domain is found in a superfamily of enzymes with a predicted or known phosphohydrolase activity. These enzymes appear to be involved in the nucleic acid metabolism, signal transduction and possibly other functions in bacteria,archaea and eukaryotes TREMBL:Q8HAA3: 41% identity,52% similarity InterPro:IPR003607; Met_phsphohydro. SMART:SM00471; HDc hstdl_phs_rel: histidinol phosphatase-r No signal peptide No transmembrane helices; Function unclear; hypothetical protein 2248190 4609692 azo2048 Azoarcus sp. BH72 hypothetical protein YP_933552.1 2247594 D 62928 CDS YP_933553.1 119898340 4607202 2248683..2248952 1 NC_008702.1 Conserved hypothetical protein. Homology to BTH_I1915 of Burkholderia thailandensis of 45%. Pfam: Prophage CP4-57 regulatory protein (AlpA)., This family consists of several short bacterial and phage proteins which are related to the E. coli protein AlpA. AlpA suppress two phenotypes of a delta lon protease mutant,overproduction of capsular polysaccharide and sensitivity to UV light. Several of the sequences in this family are thought to be DNA-binding proteins. No signal peptide. No TMHs; hypothetical protein 2248952 4607202 azo2049 Azoarcus sp. BH72 hypothetical protein YP_933553.1 2248683 D 62928 CDS YP_933554.1 119898341 4607203 complement(2249016..2250140) 1 NC_008702.1 Putative H repeat-associated protein PF01609 Transposase_11, Transposase DDE domain. Transposases are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4, IS421, IS5377, IS427, IS402, IS1355, IS5, which was original isolated in bacteriophage lambda. SignalP reporting signal Peptide to be present No TMH reproted as present.; High confidence in function and specificity; H repeat-containing protein 2250140 4607203 azo2050 Azoarcus sp. BH72 H repeat-containing protein YP_933554.1 2249016 R 62928 CDS YP_933555.1 119898342 4607204 complement(2250271..2251566) 1 NC_008702.1 Integrase. INTEGRASE IS NECESSARY FOR INTEGRATION OF THE PHAGE INTO THE HOST GENOME BY SITE-SPECIFIC RECOMBINATION. IN CONJUNCTION WITH EXCISIONASE INTEGRASE IS ALSO NECESSARY FOR EXCISION OF THE PROPHAGE FROM THE HOST GENOME. InterPro: Phage integrase; High confidence in function and specificity; phage integrase family site specific recombinase 2251566 4607204 azo2051 Azoarcus sp. BH72 phage integrase family site specific recombinase YP_933555.1 2250271 R 62928 CDS YP_933556.1 119898343 4607205 2251818..2254355 1 NC_008702.1 This protein performs the mismatch recognition step during the DNA repair process; DNA mismatch repair protein MutS 2254355 4607205 azo2052 Azoarcus sp. BH72 DNA mismatch repair protein MutS YP_933556.1 2251818 D 62928 CDS YP_933557.1 119898344 4607206 2254352..2255107 1 NC_008702.1 Spermidine synthase (Putrescine aminopropyltransferase),; Conserved hypothetical protein; putative spermidine synthase 2255107 speE1 4607206 speE1 Azoarcus sp. BH72 putative spermidine synthase YP_933557.1 2254352 D 62928 CDS YP_933558.1 119898345 4609887 complement(2255112..2255831) 1 NC_008702.1 DNA polymerase III epsilon chain. DNA POLYMERASE III IS A COMPLEX MULTICHAIN ENZYME RESPONSIBLE FOR MOST OF THE REPLICATIVE SYNTHESIS IN BACTERIA. THE EPSILON SUBUNIT CONTAIN THE EDITING FUNCTION AND IS A PROOFREADING 3-5 EXONUCLEASE (BY SIMILARITY). InterPro: Exonuclease. TIGRFAM: dnaq: DNA polymerase III epsilon chain.; High confidence in function and specificity; DNA-directed DNA polymerase 2255831 dnaQ 4609887 dnaQ Azoarcus sp. BH72 DNA-directed DNA polymerase YP_933558.1 2255112 R 62928 CDS YP_933559.1 119898346 4608639 complement(2255849..2256307) 1 NC_008702.1 Ribonuclease H (RNase H). This enzyme is an endonuclease that degrades the RNA of RNA-DNA hybrids specifically (By similarity).; High confidence in function and specificity; ribonuclease H 2256307 rnhA2 4608639 rnhA2 Azoarcus sp. BH72 ribonuclease H YP_933559.1 2255849 R 62928 CDS YP_933560.1 119898347 4609753 complement(2256308..2257072) 1 NC_008702.1 Similar to TREMBL:Q82XV9 (41% identity, generic methyl-transferase); TREMBL:Q7NYL9 (45% identity).; hypothetical protein 2257072 4609753 azo2056 Azoarcus sp. BH72 hypothetical protein YP_933560.1 2256308 R 62928 CDS YP_933561.1 119898348 4607207 2257071..2257832 1 NC_008702.1 Probable hydroxyacylglutathione hydrolase (EC 3.1.2.6) (Glyoxalase II) (Glx II). Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid.Similarity to bacterial stress protein TerD. 49% identity and 64% similarity to similar product from Nitrosomonas europaea ATCC InterPro: Metallo-beta-lactamase superfamily CoA_E_activ: CoA-substrate-specific enz; Family membership; hydroxyacylglutathione hydrolase 2257832 gloB2 4607207 gloB2 Azoarcus sp. BH72 hydroxyacylglutathione hydrolase YP_933561.1 2257071 D 62928 CDS YP_933562.1 119898349 4608910 complement(2257836..2258222) 1 NC_008702.1 Probable response regulator,; Specificity unclear; response regulator 2258222 ccdB 4608910 ccdB Azoarcus sp. BH72 response regulator YP_933562.1 2257836 R 62928 CDS YP_933563.1 119898350 4608443 complement(2258225..2259202) 1 NC_008702.1 Probable REC/GGDEF-domain containing protein,; Function unclear; REC/GGDEF-domain-containing protein 2259202 wspR1 4608443 wspR1 Azoarcus sp. BH72 REC/GGDEF-domain-containing protein YP_933563.1 2258225 R 62928 CDS YP_933564.1 119898351 4610115 2259430..2260878 1 NC_008702.1 Region start changed from 2259236 to 2259431 (-195 bases); membrane-bound lytic murein transglycosylase D 2260878 4610115 azo2060 Azoarcus sp. BH72 membrane-bound lytic murein transglycosylase D YP_933564.1 2259430 D 62928 CDS YP_933565.1 119898352 4607208 complement(2260891..2262891) 1 NC_008702.1 Hypothetical ABC transporter ATP-binding protein yliA. PROBABLY PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM YLIABCD. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. TREMBL:Q82S74: 52% identity, 64% similarity. InterPro: IPR003593; AAA_ATPase. No signal peptide No transmembrane helices. IPR003439; ABC_transporter. IPR010066; Oligo_HPY. Pfam:PF00005; ABC_tran; 2. ProDom: PD000006; ABC_transporter; 1. SMART:SM00382; AAA; 2. TIGRFAMs TIGR01727; oligo_HPY mobB: molybdopterin-guanine dinucleotid; High confidence in function and specificity; ABC transporter ATP-binding protein 2262891 dppF 4607208 dppF Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_933565.1 2260891 R 62928 CDS YP_933566.1 119898353 4608647 complement(2262996..2263481) 1 NC_008702.1 Conserved hypothetical peptidyl-prolyl cis-trans isomerase. Homology to slyD of E. coli of 41% (sprot|SLYD_ECOLI) but around 30 aa at the C-terminus are missing. Peptidylprolyl isomerases accelerate protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. InterPro: FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase) (IPR001179). Pfam: FKBP-type peptidyl-prolyl cis-trans isomerase. no signal peptide. no TMHs; Family membership; peptidyl-prolyl cis-trans isomerase 2263481 4608647 azo2062 Azoarcus sp. BH72 peptidyl-prolyl cis-trans isomerase YP_933566.1 2262996 R 62928 CDS YP_933567.1 119898354 4607209 complement(2263441..2263950) 1 NC_008702.1 Putative bacterioferritin comigratory protein. Homology to bcp of E. coli of 37% (srot:BCP_ECOLI) Interpro: Alkyl hydroperoxid reductase/ Thiol specific antioxidant / Mal Alergern (IPR000866) Pfam: AhpcC/TSA family no signal peptide no TMH; Family membership; putative bacterioferritin comigratory protein 2263950 bcp1 4607209 bcp1 Azoarcus sp. BH72 putative bacterioferritin comigratory protein YP_933567.1 2263441 R 62928 CDS YP_933568.1 119898355 4608379 2264217..2264825 1 NC_008702.1 Represses a number of genes involved in the response to DNA damage; LexA repressor 2264825 lexA 4608379 lexA Azoarcus sp. BH72 LexA repressor YP_933568.1 2264217 D 62928 CDS YP_933569.1 119898356 4609134 2264839..2265489 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. No signal peptide. 4 TMHs; hypothetical protein 2265489 4609134 azo2065 Azoarcus sp. BH72 hypothetical protein YP_933569.1 2264839 D 62928 CDS YP_933570.1 119898357 4607210 2265492..2266883 1 NC_008702.1 The MATE family consists of probable efflux proteins including a functionally characterized multi drug efflux system from Vibrio parahaemolyticus, a putative ethionine resistance protein of Saccharomyces cerevisiae,and the functionally uncharacterized DNA damage-inducible protein F (DinF) of E. coli. Functions as a Na(+)/drug antiporter. These proteins have 12 probable TMS,TREMBL:Q7NXN3 (35% identity); SWISSPROT:Q9K015 (32% identity). Pfam (UPF0013): Uncharacterized membrane protein family. TIGRFAM (TIGR00797): matE. TMHMM reporting 11 transmembrane helices. TC (2.A.66.1.): The Multi Antimicrobial Extrusion (MATE) Family.; High confidence in function and specificity; multidrug resistance protein 2266883 norM 4607210 norM Azoarcus sp. BH72 multidrug resistance protein YP_933570.1 2265492 D 62928 CDS YP_933571.1 119898358 4609398 complement(2266889..2267239) 1 NC_008702.1 Arsenate reductase (EC 1.20.4.1) (Arsenical pump modifier). REDUCTION OF ARSENATE [AS(V)] TO ARSENITE [AS(III)]. THIS PROTEIN EXPANDS THE SUBSTRATE SPECIFICITY OF ARSAB PUMP WHICH CAN EXTRUDE ARSENITE AND ANTIMONITE TO ALLOW FOR ARSENATE PUMPING AND RESISTANCE. arsC: arsenate reductase; High confidence in function and specificity; arsenate reductase 2267239 4609398 azo2067 Azoarcus sp. BH72 arsenate reductase YP_933571.1 2266889 R 62928 CDS YP_933572.1 119898359 4607211 complement(2267239..2269050) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc2890 of R. solanacearum of 53% (trembl|Q8XVE1). InterPro: PDZ domain (also known as DHR or GLGF)(IPR001478). Pfam: PDZ domain (also known as DHR or GLGF); M61 glycyl aminopeptidase PDZ domains are found in diverse signaling proteins in bacteria, yeasts, plants, insects and vertebrates. They may function in targeting signalling molecules to sub-membranous sites. Some PDZs have been shown to bind C-terminal polypeptides; others appear to bind internal (non-C-terminal) polypeptides. Different PDZs possess different binding specificities. Glycyl aminopeptidase is an unusual peptidase in that it has a preference for substrates with an N-terminal glycine or alanine. no signal peptide. no TMHs.; Family membership; hypothetical protein 2269050 4607211 azo2068 Azoarcus sp. BH72 hypothetical protein YP_933572.1 2267239 R 62928 CDS YP_933573.1 119898360 4610028 complement(2269613..2271997) 1 NC_008702.1 Region start changed from 2272064 to 2271998 (-66 bases); ATP-dependent protease La 2271997 4610028 azo2069 Azoarcus sp. BH72 ATP-dependent protease La YP_933573.1 2269613 R 62928 CDS YP_933574.1 119898361 4607213 complement(2272107..2273375) 1 NC_008702.1 binds and unfolds substrates as part of the ClpXP protease; ATP-dependent protease ATP-binding subunit ClpX 2273375 clpX 4607213 clpX Azoarcus sp. BH72 ATP-dependent protease ATP-binding subunit ClpX YP_933574.1 2272107 R 62928 CDS YP_933575.1 119898362 4608491 complement(2273393..2274031) 1 NC_008702.1 hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit 2274031 clpP 4608491 clpP Azoarcus sp. BH72 ATP-dependent Clp protease proteolytic subunit YP_933575.1 2273393 R 62928 CDS YP_933576.1 119898363 4608489 complement(2274050..2275363) 1 NC_008702.1 Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer; trigger factor 2275363 tig 4608489 tig Azoarcus sp. BH72 trigger factor YP_933576.1 2274050 R 62928 CDS YP_933577.1 119898364 4609947 2275699..2277621 1 NC_008702.1 Putative serine protein kinase. Homology to prkA of Bacillus subtilis of 32% (gnl|keqq|bsu:BG10804 ). Pfam: PrkA serine kinase. This is a family of PrkA bacterial and archaeal serine kinases approximately 630 residues long. PrkA possesses the A-motif of nucleotide-binding proteins and exhibits distant homology to eukaryotic protein kinases. Note that many family members are hypothetical. Interpro: IPR010650 PrkA serine kinase. No signal peptide. No TMHs.,; Family membership; PrkA protein 2277621 prkA 4609947 prkA Azoarcus sp. BH72 PrkA protein YP_933577.1 2275699 D 62928 CDS YP_933578.1 119898365 4609628 2277682..2278950 1 NC_008702.1 Conserved hypothetical protein,[yeaH]54% identical (71% similarity )to SwissProt;P59349. Has PF04285(IPR006698), Protein of unknown function (DUF444);Bacterial protein of unknown function. One family member (Q97LI1) is predicted to contain a von Willebrand factor (vWF) type A domain (Smart:VWA). No Signal peptide or TMH present.; hypothetical protein 2278950 yeaH 4609628 yeaH Azoarcus sp. BH72 hypothetical protein YP_933578.1 2277682 D 62928 CDS YP_933579.1 119898366 4610165 2278947..2280482 1 NC_008702.1 Putative cytoplasmic protein[ycgB],67% identical (79% similarity) SwissProt;P29013. TrEMBL;Q8XDL3(67% identical)TrEMBL;Q8ZP16. Has PF04293, SpoVR like protein;IPR007390; Family member P37875 is Bacillus subtilis stage V sporulation protein R,which is involved in spore cortex formation. Little is known about cortex biosynthesis, except that it depends on several sigma E controlled genes, including spoVR. No Signal peptide or TMH present.; Family membership; SpoVR family protein 2280482 ycgB 4610165 ycgB Azoarcus sp. BH72 SpoVR family protein YP_933579.1 2278947 D 62928 CDS YP_933580.1 119898367 4610158 2280524..2282053 1 NC_008702.1 38% UPF0031.IPR004443; YjeF_Nterm. Pfam:PF01256; Carb_kinase; 1.PF03853; YjeF_N; 1. TIGRFAMs:TIGR00196; yjeF_cterm; 1.TIGR00197; yjeF_nterm; 1.; Specificity unclear; hypothetical protein 2282053 4610158 azo2076 Azoarcus sp. BH72 hypothetical protein YP_933580.1 2280524 D 62928 CDS YP_933581.1 119898368 4607214 2282131..2283384 1 NC_008702.1 HD-domain containing protein,; Conserved hypothetical protein; HD-domain-containing protein 2283384 4607214 azo2077 Azoarcus sp. BH72 HD-domain-containing protein YP_933581.1 2282131 D 62928 CDS YP_933582.1 119898369 4607215 2283461..2284315 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA0239 pf P. aeruginosa of 41%. InterPro: Integral membrane protein DUF6. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transport. Pfam: Integral membrane protein DUF6. 10 TMHs. no signal peptide; Conserved hypothetical protein; hypothetical protein 2284315 4607215 azo2078 Azoarcus sp. BH72 hypothetical protein YP_933582.1 2283461 D 62928 CDS YP_933583.1 119898370 4607216 2284399..2285247 1 NC_008702.1 Nicotinate-nucleotide pyrophosphorylase [carboxylating] (Quinolinate phosphoribosyltransferase [decarboxylating]) (QAPRTase). TIGRFAM: nadC: nicotinate-nucleotide pyrophosphorylase; High confidence in function and specificity; nicotinate-nucleotide diphosphorylase 2285247 nadC 4607216 nadC Azoarcus sp. BH72 nicotinate-nucleotide diphosphorylase YP_933583.1 2284399 D 62928 CDS YP_933584.1 119898371 4609304 complement(2285402..2286865) 1 NC_008702.1 catalyzes the formation of L-threonine from O-phospho-L-homoserine; threonine synthase 2286865 thrC 4609304 thrC Azoarcus sp. BH72 threonine synthase YP_933584.1 2285402 R 62928 CDS YP_933585.1 119898372 4609944 complement(2287025..2288332) 1 NC_008702.1 catalyzes the formation of L-aspartate 4-semialdehyde from L-homoserine; homoserine dehydrogenase 2288332 hom 4609944 hom Azoarcus sp. BH72 homoserine dehydrogenase YP_933585.1 2287025 R 62928 CDS YP_933586.1 119898373 4609015 complement(2288377..2289705) 1 NC_008702.1 broad specificity; family IV; in Corynebacterium glutamicum this protein can use glutamate, 2-aminobutyrate, and aspartate as amino donors and pyruvate as the acceptor; aminotransferase AlaT 2289705 yfbQ 4609015 yfbQ Azoarcus sp. BH72 aminotransferase AlaT YP_933586.1 2288377 R 62928 CDS YP_933587.1 119898374 4610170 2289870..2290250 1 NC_008702.1 Conserved hypothetical protein. Homology to RS02847 of R.solanacearum of 49% (trembl:Q8XZS0). Has PF04635,Protein of unknown function, DUF598;IPR006729; This family contains several uncharacterised proteins. No signal peptide or TMH present.; hypothetical protein 2290250 4610170 azo2083 Azoarcus sp. BH72 hypothetical protein YP_933587.1 2289870 D 62928 CDS YP_933588.1 119898375 4607217 complement(2290256..2291482) 1 NC_008702.1 Member of the Major Facilitator Superfamily (MFS). MFS transporters are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. Hypothetical transport protein yjjL. InterPro: General substrate transporters 2A0104: phosphoglycerate transporter; Function unclear; putative membrane transport protein 2291482 4607217 azo2084 Azoarcus sp. BH72 putative membrane transport protein YP_933588.1 2290256 R 62928 CDS YP_933589.1 119898376 4607218 complement(2291561..2293009) 1 NC_008702.1 Probable xanthan biosynthesis protein XanB. Homology to xanB of X. campestris of 60% (sprot|XANB_XANCP(SRS)) Xanthan biosynthesis protein XanB is a multifunctional enzyme including Mannose-6-phosphate isomerase (EC 5.3.1.8) (Phosphomannose isomerase) (PMI) (Phosphohexomutase)and Mannose-1-phosphate guanylyl transferase (GDP) (EC 2.7.7.22) (GDP-mannose pyrophosphorylase) (GMP). InterPro: ADP-glucose pyrophosphorylase (IPR005835); Mannose-6-phosphate isomerase type II (IPR001538) Pfam: Nucleotidyl transferase, Mannose-6-phosphate isomerase no signal peptide no TMHs; Family membership; xanthan biosynthesis protein XanB 2293009 xanB 4607218 xanB Azoarcus sp. BH72 xanthan biosynthesis protein XanB YP_933589.1 2291561 R 62928 CDS YP_933590.1 119898377 4610122 complement(2293152..2293907) 1 NC_008702.1 Putative transcriptional activator protein,; Function unclear; putative transcriptional activator protein 2293907 4610122 azo2086 Azoarcus sp. BH72 putative transcriptional activator protein YP_933590.1 2293152 R 62928 CDS YP_933591.1 119898378 4607219 complement(2293904..2294440) 1 NC_008702.1 In Pseudomonas putida strain GB-1, XcmX is involved in a novel Xcp-related system for the transport of manganese-oxidizing enzymes to the cell surface. Similar trembl|Q8KSG3 (33%). TMHMM reporting one TMH. SignalP reporting a signal peptid.; Function unclear; putative Gsp-related pathway protein 2294440 xcmX 4607219 xcmX Azoarcus sp. BH72 putative Gsp-related pathway protein YP_933591.1 2293904 R 62928 CDS YP_933592.1 119898379 4610123 complement(2294427..2294819) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pp3476 of P. putida of 54% (tremble:Q88H85). no domains predicted. TMH in signal peptide. Has Signal Peptide.; Conserved hypothetical protein; hypothetical protein 2294819 4610123 azo2088 Azoarcus sp. BH72 hypothetical protein YP_933592.1 2294427 R 62928 CDS YP_933593.1 119898380 4607220 complement(2294825..2295301) 1 NC_008702.1 General secretion pathway protein G,; Specificity unclear; general secretion pathway protein G 2295301 gspG1 4607220 gspG1 Azoarcus sp. BH72 general secretion pathway protein G YP_933593.1 2294825 R 62928 CDS YP_933594.1 119898381 4608954 complement(2295298..2297325) 1 NC_008702.1 Protein D is involved in the type II general secretion pathway within Gram-negative bacteria, a signal sequence-dependent process responsible for protein export. The most probable location of protein D is the outer membrane. This suggests that protein D constitutes the apparatus of the accessory mechanism, and is thus involved in transporting exoproteins from the periplasm, across the outer membrane, to the extracellular environment. Similar to trembl|Q8XTG8 (39%). Pfam (PF00263): General (type II) secretion pathway (GSP) D protein Pfam (PF07660): Secretin and TonB N terminus short domain Pfam (PF03958): Bacterial type II/III secretion system short domain SignalP reporting Signal peptide.; Specificity unclear; general secretion pathway protein D 2297325 gspD2 4608954 gspD2 Azoarcus sp. BH72 general secretion pathway protein D YP_933594.1 2295298 R 62928 CDS YP_933595.1 119898382 4608948 complement(2297322..2297813) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rs02976 of R. solanacearum of 35% (trembl|Q8XTG7(SRS)). No domains predicted. Signal peptide present. no TMHs; Conserved hypothetical protein; hypothetical protein 2297813 4608948 azo2091 Azoarcus sp. BH72 hypothetical protein YP_933595.1 2297322 R 62928 CDS YP_933596.1 119898383 4607221 complement(2297810..2298373) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA1234 of Azoarcus sp. EbN1 of 38% (gnl|keqq|eba:ebA1234(KEGG)). no domains predicted. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2298373 4607221 azo2092 Azoarcus sp. BH72 hypothetical protein YP_933596.1 2297810 R 62928 CDS YP_933597.1 119898384 4607222 complement(2298370..2298918) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA1229 of Azoarcus sp. EbN1 of 37% (gnl|keqq|eba:ebA1229(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2298918 4607222 azo2093 Azoarcus sp. BH72 hypothetical protein YP_933597.1 2298370 R 62928 CDS YP_933598.1 119898385 4607223 complement(2298915..2299715) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD34 of Azoarcus sp. EbN1 of 33% (gnl|keqq|eba:ebD34(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2299715 4607223 azo2094 Azoarcus sp. BH72 hypothetical protein YP_933598.1 2298915 R 62928 CDS YP_933599.1 119898386 4607224 complement(2299696..2301372) 1 NC_008702.1 General secretion pathway protein E (Type II traffic warden ATPase) INVOLVED IN A GENERAL SECRETION PATHWAY (GSP) FOR THE EXPORT OF PROTEINS. Similar to sprot|PILF_NEIGO (33%) and trembl|Q82U87 (54%). InterPro (PF00437): Bacterial type II secretion system protein E InterPro (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; general secretion pathway protein E 2301372 gspE2 4607224 gspE2 Azoarcus sp. BH72 general secretion pathway protein E YP_933599.1 2299696 R 62928 CDS YP_933600.1 119898387 4608950 complement(2301369..2302541) 1 NC_008702.1 General secretion pathway protein F,similar trembl|Q88HD6 (39%). The general (type II) secretion pathway (GSP) within Gram-negative bacteria is a signal sequence-dependent process responsible for protein export. The process has two stages: exoproteins are first translocated across the inner membrane by the general signal-dependent export pathway (GEP), and then across the outer membrane by a species-specific accessory mechanism. InterPro (IPR001992): Bacterial type II secretion system protein. InterPro (IPR003004): Bacterial general secretion pathway protein F Pfam (PF00482): Bacterial general secretion pathway protein F TMHMM reporting five TMH.; Specificity unclear; general secretion pathway protein F 2302541 gspF2 4608950 gspF2 Azoarcus sp. BH72 general secretion pathway protein F YP_933600.1 2301369 R 62928 CDS YP_933601.1 119898388 4608953 complement(2302565..2303020) 1 NC_008702.1 General secretion pathway GSPG-related transmembrane protein,; Specificity unclear; general secretion pathway protein G 2303020 gspG2 4608953 gspG2 Azoarcus sp. BH72 general secretion pathway protein G YP_933601.1 2302565 R 62928 CDS YP_933602.1 119898389 4608955 2303193..2303888 1 NC_008702.1 Soluble lytic murein transglycosylase precursor (EC 3.2.1.-) (Slt70). Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division (By similarity). flhB_rel: FlhB domain protein; Family membership; soluble lytic murein transglycosylase 2303888 slt 4608955 slt Azoarcus sp. BH72 soluble lytic murein transglycosylase YP_933602.1 2303193 D 62928 CDS YP_933603.1 119898390 4609873 2303995..2305512 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to xac2776 of X. axonopodis of 50% (trembl|Q8PIW9). Pfam: DUF839 This family consists of several bacterial proteins of unknown function. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2305512 4609873 azo2099 Azoarcus sp. BH72 hypothetical protein YP_933603.1 2303995 D 62928 CDS YP_933604.1 119898391 4607225 complement(2305593..2306900) 1 NC_008702.1 catalyzes the formation of pyruvate and beta-alanine from L-alanine and 3-oxopropanoate; beta alanine--pyruvate transaminase 2306900 4607225 azo2100 Azoarcus sp. BH72 beta alanine--pyruvate transaminase YP_933604.1 2305593 R 62928 CDS YP_933605.1 119898392 4607226 complement(2307012..2307671) 1 NC_008702.1 Hypothetical membrane protein. No homology with hits in the database. No domains predicted. Signal peptide present. TMHMM2 reporting 1 TMH present.; hypothetical protein 2307671 4607226 azo2101 Azoarcus sp. BH72 hypothetical protein YP_933605.1 2307012 R 62928 CDS YP_933606.1 119898393 4610015 2308537..2308899 1 NC_008702.1 DnaK suppressor protein,; High confidence in function and specificity; DksA2 protein 2308899 dksA2 4610015 dksA2 Azoarcus sp. BH72 DksA2 protein YP_933606.1 2308537 D 62928 CDS YP_933607.1 119898394 4608625 complement(2308958..2311057) 1 NC_008702.1 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Polynucleotide phosphorylase) (PNPase). Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3 to 5 direction. Involved in the RNA degradosome a multi-enzyme complex important in RNA processing and messenger RNA degradation.; High confidence in function and specificity; polynucleotide phosphorylase/polyadenylase 2311057 pnp 4608625 pnp Azoarcus sp. BH72 polynucleotide phosphorylase/polyadenylase YP_933607.1 2308958 R 62928 CDS YP_933608.1 119898395 4609589 complement(2311255..2311524) 1 NC_008702.1 primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence; 30S ribosomal protein S15 2311524 rpsO 4609589 rpsO Azoarcus sp. BH72 30S ribosomal protein S15 YP_933608.1 2311255 R 62928 CDS YP_933609.1 119898396 4609818 complement(2311618..2312520) 1 NC_008702.1 catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs; tRNA pseudouridine synthase B 2312520 truB 4609818 truB Azoarcus sp. BH72 tRNA pseudouridine synthase B YP_933609.1 2311618 R 62928 CDS YP_933610.1 119898397 4610038 complement(2312537..2312917) 1 NC_008702.1 associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock; ribosome-binding factor A 2312917 rbfA 4610038 rbfA Azoarcus sp. BH72 ribosome-binding factor A YP_933610.1 2312537 R 62928 CDS YP_933611.1 119898398 4607228 complement(2312921..2315743) 1 NC_008702.1 Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; translation initiation factor IF-2 2315743 infB 4607228 infB Azoarcus sp. BH72 translation initiation factor IF-2 YP_933611.1 2312921 R 62928 CDS YP_933612.1 119898399 4609083 complement(2315757..2317232) 1 NC_008702.1 modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination; transcription elongation factor NusA 2317232 nusA 4609083 nusA Azoarcus sp. BH72 transcription elongation factor NusA YP_933612.1 2315757 R 62928 CDS YP_933613.1 119898400 4609433 complement(2317260..2317694) 1 NC_008702.1 in Streptococcus pneumoniae this gene was found to be essential; structure determination of the Streptococcus protein shows that it is similar to a number of other proteins; hypothetical protein 2317694 4609433 azo2109 Azoarcus sp. BH72 hypothetical protein YP_933613.1 2317260 R 62928 CDS YP_933614.1 119898401 4607229 complement(2317918..2318988) 1 NC_008702.1 Conserved hypothetical protein; Family membership; pseudouridylate synthase 2318988 4607229 azo2110 Azoarcus sp. BH72 pseudouridylate synthase YP_933614.1 2317918 R 62928 CDS YP_933615.1 119898402 4607230 complement(2318924..2319532) 1 NC_008702.1 Putative segregation and condensation protein B,; Conserved hypothetical protein; putative segregation and condensation protein B 2319532 scpB 4607230 scpB Azoarcus sp. BH72 putative segregation and condensation protein B YP_933615.1 2318924 R 62928 CDS YP_933616.1 119898403 4609852 complement(2319510..2320340) 1 NC_008702.1 Hypothetical protein, 51% identity(64% similarity) to TrEMBL;Q8XWT4. Has PF02616, Uncharacterised ACR,COG1354;This entry describes proteins of unknown function. InterPro; IPR003768; Specificity unclear; hypothetical protein 2320340 4609852 azo2112 Azoarcus sp. BH72 hypothetical protein YP_933616.1 2319510 R 62928 CDS YP_933617.1 119898404 4607231 complement(2320366..2321025) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE1726 of N.europaea of 61% (trembl:Q82TY8). InterPro: IPR008915; Peptidase_M50. Pfam: PF02163; Peptidase_M50. Non-secretory protein signal peptide probability: 0.235. TMHMM predicted 4 transmembrane helices; Conserved hypothetical protein; hypothetical protein 2321025 4607231 azo2113 Azoarcus sp. BH72 hypothetical protein YP_933617.1 2320366 R 62928 CDS YP_933618.1 119898405 4607232 complement(2321072..2321704) 1 NC_008702.1 InterPro: Sua5/YciO/YrdC/YwlC protein family TIGR00057: Sua5/YciO/YrdC/YwlC family p; High confidence in function and specificity; hypothetical protein 2321704 4607232 azo2114 Azoarcus sp. BH72 hypothetical protein YP_933618.1 2321072 R 62928 CDS YP_933619.1 119898406 4607233 complement(2321712..2322563) 1 NC_008702.1 TREMBL:Q7VZ25: 48% identity; 57% similarity SWISSPROT:TRPH_HAEIN:Protein trpH (38% identity). InterPro:IPR003141; PHP_N.PHP domain C-terminal region. The PHP (Polymerase and Histidinol Phosphatase) domain is a putative phosphoesterase domain. This family is often associated with an N-terminal region Pfam: PF02231; PHP_N; SMART: SM00481; POLIIIAc; thiE: thiamine-phosphate pyrophosphoryl; High confidence in function and specificity; hypothetical protein 2322563 trpH 4607233 trpH Azoarcus sp. BH72 hypothetical protein YP_933619.1 2321712 R 62928 CDS YP_933620.1 119898407 4610036 complement(2322571..2323473) 1 NC_008702.1 Probable Serine hydrolase-like protein (EC 3.1.-.-). TREMBL:Q8Y0A9: 49% identity, 63% similarity. Pfam: abhydrolase; thioesterase TIGRFAM: murB: UDP-N-acetylenolpyruvoylglucosamine No transmembrane helix (predicted by TMHMM); Function unclear; hydrolase or acytransferase 2323473 4610036 azo2116 Azoarcus sp. BH72 hydrolase or acytransferase YP_933620.1 2322571 R 62928 CDS YP_933621.1 119898408 4607234 2323493..2323834 1 NC_008702.1 Hypothetical protein. Very bad homology with hits. Has No domains, repeats, motifs or features detected.; hypothetical protein 2323834 4607234 azo2117 Azoarcus sp. BH72 hypothetical protein YP_933621.1 2323493 D 62928 CDS YP_933622.1 119898409 4607235 2323896..2324195 1 NC_008702.1 Conserved hypothetical protein. Very Bad homology with hits in the database. Has SMART;SM00438;IPR000967,Znf_NFX1;This domain is presumed to be a zinc binding domain. The following pattern describes the zinc finger: C-X(1-6)-H-X-C-X3-C(H/C)-X(3-4)-(H/C)-X(1-10)-C, where X can be any amino acid, and numbers in brackets indicate the number of residues. The two position can be either his or cys. This domain is found in the human transcriptional repressor NK-X1, a repressor of HLA-DRA transcription; the Drosophila shuttle craft protein, which plays an essential role during the late stages of embryonic neurogenesis; and a yeast hypothetical protein YNL023C.; hypothetical protein 2324195 4607235 azo2118 Azoarcus sp. BH72 hypothetical protein YP_933622.1 2323896 D 62928 CDS YP_933623.1 119898410 4607236 2324230..2325735 1 NC_008702.1 probable piperideine-6-carboxylate dehydrogenase. Homology to pcd of F. lutescens of 53% (trembl|Q9F1U8) InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; Family membership; piperideine-6-carboxylate dehydrogenase 2325735 pcD 4607236 pcD Azoarcus sp. BH72 piperideine-6-carboxylate dehydrogenase YP_933623.1 2324230 D 62928 CDS YP_933624.1 119898411 4609508 complement(2325732..2326607) 1 NC_008702.1 Hypothetical protein MG442 homolog (K05_orf271). TREMBL:Q7NR72: 48% identity, 66% similarity InterPro; IPR005289; GTP-bindding_dom. InterPro; IPR002917; MMR_HSR1. Pfam; PF01926; MMR_HSR1; thiE: thiamine-phosphate pyrophosphor NO signal peptide No transmembrane helices; Specificity unclear; GTPase 2326607 ylqF 4609508 ylqF Azoarcus sp. BH72 GTPase YP_933624.1 2325732 R 62928 CDS YP_933625.1 119898412 4610193 2326914..2327123 1 NC_008702.1 Cold shock-like protein,; High confidence in function and specificity; cold shock-like protein 2327123 cspA 4610193 cspA Azoarcus sp. BH72 cold shock-like protein YP_933625.1 2326914 D 62928 CDS YP_933626.1 119898413 4608538 2327305..2329257 1 NC_008702.1 Cold-shock DEAD-box protein A (ATP-dependent RNA helicase deaD). HAS A HELIX-DESTABILIZING ACTIVITY. PLAYS A KEY ROLE IN OPTIMAL CELL GROWTH AT LOW TEMPERATURE AND IS REQUIRED FOR NORMAL CELL DIVISION (BY SIMILARITY).; Family membership; ATP-dependent RNA helicase 2329257 deaD 4608538 deaD Azoarcus sp. BH72 ATP-dependent RNA helicase YP_933626.1 2327305 D 62928 CDS YP_933627.1 119898414 4608614 2329257..2329883 1 NC_008702.1 Putative acyl-CoA thioesterase. Homology to tesA of E. coli of 32% (sprot|TESA_ECOLI) Hydrolyzes long chain acyl thioesters (c12-c18): 2-lysophosphatidylcholine + h(2)o = glycerophosphocholine + a fatty acid anion. InterPro: GDSL lipolytic enzyme (IPR001087) Pfam: Lipase/Acylhydrolase GDSL-like motif signal peptide no TMH; Family membership; putative acyl-CoA thioesterase I 2329883 tesA2 4608614 tesA2 Azoarcus sp. BH72 putative acyl-CoA thioesterase I YP_933627.1 2329257 D 62928 CDS YP_933628.1 119898415 4609929 2329949..2330935 1 NC_008702.1 catalyzes the phosphorylation of protein substrates at serine and threonine residues in vitro; specific substrate in vivo has not been identified yet; plays a role in long-term cell survival and expression of surface appendages; serine/threonine protein kinase 2330935 4609929 azo2124 Azoarcus sp. BH72 serine/threonine protein kinase YP_933628.1 2329949 D 62928 CDS YP_933629.1 119898416 4607237 2331002..2331898 1 NC_008702.1 TREMBL:Q9HT60:67% identity, 80% similarity to putative hydrolase. Hypothetical protein yqjL. InterPro: Alpha/beta hydrolase fold Pfam: abhydrolase; thioesterase domain uppS: undecaprenyl diphosphate synthase; Specificity unclear; hypothetical protein 2331898 4607237 azo2125 Azoarcus sp. BH72 hypothetical protein YP_933629.1 2331002 D 62928 CDS YP_933630.1 119898417 4607238 complement(2331887..2332435) 1 NC_008702.1 Putative cytoplasmic protein [yaeQ],49% identity (64% similarity) to TrEMBL;Q79ID7. TrEMBL;Q8FKZ7(57% identity) SwissProt;P52100. Has PF07152, YaeQ protein;This family consists of several hypothetical bacterial proteins of around 180 residues in length which are often known as YaeQ. YaeQ is homologous to RfaH, a specialised transcription elongation protein. YaeQ is known to compensate for loss of RfaH function. No Signal Peptide or TMH present.; High confidence in function and specificity; putative cytoplasmic protein 2332435 yaeQ 4607238 yaeQ Azoarcus sp. BH72 putative cytoplasmic protein YP_933630.1 2331887 R 62928 CDS YP_933631.1 119898418 4610137 complement(2332443..2333228) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA6182 Azoarcus sp. EbN1 of 55% (gnl|keqq|eba:ebA6182(KEGG)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 2333228 4610137 azo2127 Azoarcus sp. BH72 hypothetical protein YP_933631.1 2332443 R 62928 CDS YP_933632.1 119898419 4607239 2333487..2334902 1 NC_008702.1 GGDEF-domain containing protein,; Conserved hypothetical protein; diguanylate cyclase 2334902 4607239 azo2128 Azoarcus sp. BH72 diguanylate cyclase YP_933632.1 2333487 D 62928 CDS YP_933633.1 119898420 4607240 complement(2334907..2335401) 1 NC_008702.1 Hypothetical protein, 32% identity to TrEMBL;Q6MXJ8. No Signal Peptide, features or Domains present.; hypothetical protein 2335401 4607240 azo2129 Azoarcus sp. BH72 hypothetical protein YP_933633.1 2334907 R 62928 CDS YP_933634.1 119898421 4607241 2335612..2336391 1 NC_008702.1 Catalytic activity :-acyl-[acyl-carrier protein] + nad(+) = trans- 2,3-dehydroacyl-[acyl-carrier protein] + nadh. pathway:- fatty acid biosynthesis pathway; second reduction step. Entry name SWISSPROT:FABI_ECOLI InterPro IPR002198; ADH_short. Identities = 108/252 (42%) Pfam PF00106; adh_short; 1. Prediction: Non-secretory protein Signal peptide probability: 0.00 Number of predicted TMHs: 0; High confidence in function and specificity; enoyl-(acyl carrier protein) reductase 2336391 fabI2 4607241 fabI2 Azoarcus sp. BH72 enoyl-(acyl carrier protein) reductase YP_933634.1 2335612 D 62928 CDS YP_933635.1 119898422 4608715 2336402..2336998 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03002758 of Dechloromonas aromatica of 51% (gi|53730017|ref|ZP_00150562.2|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2336998 4608715 azo2131 Azoarcus sp. BH72 hypothetical protein YP_933635.1 2336402 D 62928 CDS YP_933636.1 119898423 4607242 complement(2337022..2337999) 1 NC_008702.1 Conserved hypothetical ATPase. Homology to Daro03000733 of Dechloromonas aromatica of 64% (gi|41725756|ref|ZP_00152514.1|(NBCI ENTREZ)). Domain structure: 101 aa - 293 aa AAA-protein. InterPro: AAA-protein (ATPases associated with various cellular activities) (IPR003959); AAA ATPase superfamily (IPR003593). Pfam: ATPases associated with various cellular activities. no signal peptide. no TMHS; Conserved hypothetical protein; ATPase 2337999 4607242 azo2132 Azoarcus sp. BH72 ATPase YP_933636.1 2337022 R 62928 CDS YP_933637.1 119898424 4607243 2338181..2338852 1 NC_008702.1 involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer; GTP cyclohydrolase I 2338852 folE 4607243 folE Azoarcus sp. BH72 GTP cyclohydrolase I YP_933637.1 2338181 D 62928 CDS YP_933638.1 119898425 4608821 complement(2338856..2339185) 1 NC_008702.1 Hypothetical protein, has no good homology over the entire length with hits in Database. TrEMBL Q9HT58 Hypothetical protein PA5515 [PA5515] [Pseudomonas aeruginosa. No Signal Peptide or TMH being reported as present.; Family membership; hypothetical protein 2339185 4608821 azo2134 Azoarcus sp. BH72 hypothetical protein YP_933638.1 2338856 R 62928 CDS YP_933639.1 119898426 4607244 complement(2339196..2340776) 1 NC_008702.1 Probable low-affinity phosphate transporter protein.Involved in phosphate transport depending on the proton motive force. InterPro: Phosphate transporter family Similar to PitA a cation-phosphate transporter in E.coli. No signal peptide; High confidence in function and specificity; putative phosphate permease 2340776 4607244 azo2135 Azoarcus sp. BH72 putative phosphate permease YP_933639.1 2339196 R 62928 CDS YP_933640.1 119898427 4607245 complement(2340834..2341187) 1 NC_008702.1 Hypothetical protein ypjD. TREMBL:Q7W7V7: 56% identity, 65% similarity SPROT:YPJD_BACSU:P42979: 27% identity, 49% similarity This group of prokaryotic proteins has no known function. It includes the uncharacterized protein MazG in Escherichia coli. InterPro:IPR004518; MazG. Pfam: PF03819 No transmembrane helices (TMHMM predicted); Specificity unclear; hypothetical protein 2341187 4607245 azo2136 Azoarcus sp. BH72 hypothetical protein YP_933640.1 2340834 R 62928 CDS YP_933641.1 119898428 4607246 complement(2341245..2342090) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv2345 of C. violaceum of 54% (trembl|Q7NVJ7). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 2342090 4607246 azo2137 Azoarcus sp. BH72 hypothetical protein YP_933641.1 2341245 R 62928 CDS YP_933642.1 119898429 4607247 2342306..2342851 1 NC_008702.1 Hypothetical protein PA3753. A variety of bacterial transferases contain a repeat structure composed of tandem repeats of a [LIV]-G-X(4) hexapeptide, which, in the tertiary structure of LpxA (UDP N-acetylglucosamine acyltransferase) [1], has been shown to form a left-handed parallel beta helix TREMBL:Q9A526: 55% identity; 75% similarity bacterial transferase family protein. Superfamily: ferripyochelin binding protein InterPro:IPR001451; Hexapep_transf.Bacterial transferase hexapeptide repeat Pfam:PF00132; Hexapep; presence of DUF99 domain. No signal peptide (Signal P) or transmembrane helices (TMHMM).; Specificity unclear; hypothetical protein 2342851 4607247 azo2138 Azoarcus sp. BH72 hypothetical protein YP_933642.1 2342306 D 62928 CDS YP_933643.1 119898430 4607248 2342971..2343666 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV3305 of C.violaceum of 37% (tremble:Q7NSW4). Has PF04402, Protein of unknown function (DUF541);IPR007497:Members of this family have so far been found in bacteria and mouse SwissProt or TrEMBL entries. However possible family members have also been identified in translated rat (Genbank:AW144450) and human (Genbank:AI478629) ESTs. A mouse family member has been named SIMPL (signalling molecule that associates with mouse pelle-like kinase). SIMPL appears to facilitate and/or regulate complex formation between IRAK/mPLK (IL-1 receptor-associated kinase) and IKK (inhibitor of kappa-B kinase) containing complexes, and thus regulate NF-kappa-B activity. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 2343666 4607248 azo2139 Azoarcus sp. BH72 hypothetical protein YP_933643.1 2342971 D 62928 CDS YP_933644.1 119898431 4607249 2343704..2344009 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebD110 of Azoarcus sp. EbN1 of 46% (gnl|keqq|eba:ebD110(KEGG)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 2344009 4607249 azo2140 Azoarcus sp. BH72 hypothetical protein YP_933644.1 2343704 D 62928 CDS YP_933645.1 119898432 4607250 2344066..2344623 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV2523 of Chromobacterium violaceum of 48% (trembl:Q7NV23). Pfam: NlpC/P60 family. The function of this domain is unknown. It is found in several lipoproteins. Signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 2344623 4607250 azo2141 Azoarcus sp. BH72 hypothetical protein YP_933645.1 2344066 D 62928 CDS YP_933646.1 119898433 4607251 2344733..2345164 1 NC_008702.1 Hypothetical protein MK0525 (OrfX). trembl|Q9V230 (Q9V230) Inosine-5'-monophosphate dehydrogenase related; 34% identity, 55% similarity. InterPro: CBS domain (IPR005857, IPR004840) TIGRFAM: kpsF/GutQ family TMHMM predicted absence of transmembrane helices; Specificity unclear; putative inosine-5'-monophosphate dehydrogenase related protein 2345164 4607251 azo2142 Azoarcus sp. BH72 putative inosine-5'-monophosphate dehydrogenase related protein YP_933646.1 2344733 D 62928 CDS YP_933647.1 119898434 4607252 complement(2345219..2345494) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV3460 of C. violaceum of 54% (trembl|Q7NSG6(SRS)). Pfam: Septum formation initiator DivIC from B. subtilis is necessary for both vegetative and sporulation septum formation. These proteins are mainly composed of an amino terminal coiled-coil. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2345494 4607252 azo2143 Azoarcus sp. BH72 hypothetical protein YP_933647.1 2345219 R 62928 CDS YP_933648.1 119898435 4607253 complement(2345585..2346874) 1 NC_008702.1 enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis; phosphopyruvate hydratase 2346874 eno 4607253 eno Azoarcus sp. BH72 phosphopyruvate hydratase YP_933648.1 2345585 R 62928 CDS YP_933649.1 119898436 4608670 complement(2346959..2347798) 1 NC_008702.1 catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis; 2-dehydro-3-deoxyphosphooctonate aldolase 2347798 kdsA 4608670 kdsA Azoarcus sp. BH72 2-dehydro-3-deoxyphosphooctonate aldolase YP_933649.1 2346959 R 62928 CDS YP_933650.1 119898437 4609103 complement(2347810..2349450) 1 NC_008702.1 CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer; CTP synthetase 2349450 pyrG 4609103 pyrG Azoarcus sp. BH72 CTP synthetase YP_933650.1 2347810 R 62928 CDS YP_933651.1 119898438 4609673 2349604..2350314 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. Signal peptide present. TMHMM2 reporting 3 TMH present.; hypothetical protein 2350314 4609673 azo2147 Azoarcus sp. BH72 hypothetical protein YP_933651.1 2349604 D 62928 CDS YP_933652.1 119898439 4607254 complement(2350311..2351624) 1 NC_008702.1 L-sorbosone dehydrogenase. Converts L-sorbosone to 2-keto-l-gulonic acid (2kga). 63% Peptidase_M14.IPR000595; cNMP_binding. Signal peptide:present.; High confidence in function and specificity; putative L-sorbosone dehydrogenase 2351624 sndH 4607254 sndH Azoarcus sp. BH72 putative L-sorbosone dehydrogenase YP_933652.1 2350311 R 62928 CDS YP_933653.1 119898440 4609879 complement(2351727..2353712) 1 NC_008702.1 AcsA; in Sinorhizobium meliloti this enzyme is required for acetoacetate activation; similar to acetyl-CoA synthase; acetoacetyl-CoA synthetase 2353712 acsA 4609879 acsA Azoarcus sp. BH72 acetoacetyl-CoA synthetase YP_933653.1 2351727 R 62928 CDS YP_933654.1 119898441 4608274 complement(2353748..2354155) 1 NC_008702.1 Probable Hypothetical protein AQ_1494. TREMBL:Q7WIS5: 37% identity; 51% similarity. This family contains a wide variety of enzymes, principally thioesterases. This family includes 4HBT (EC: 3.1.2.23) which catalyses the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3. This family includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyse the hydrolysis of other long chain fatty acyl-CoA thioesters. InterPro:IPR006683; Thioestr_supf. Pfam:PF03061; 4HBT InterPro: 4-hydroxybenzoyl-CoA thioesterase family active site No transmembrane helices TIGR00051: conserved hypothetical protein; Family membership; hypothetical protein 2354155 4608274 azo2150 Azoarcus sp. BH72 hypothetical protein YP_933654.1 2353748 R 62928 CDS YP_933655.1 119898442 4607255 2354316..2355959 1 NC_008702.1 Probable electron transfer flavoprotein-ubiquinone oxidoreductase. Homology to etfdh of H. sapiens of 57% (sprot|ETFD_HUMAN) Accepts electrons and reduces ubiquinone. Pfam: FAD_binding_3; ETF_QO no signal peptide no TMHs; electron transfer flavoprotein-ubiquinone oxidoreductase 2355959 etf1 4607255 etf1 Azoarcus sp. BH72 electron transfer flavoprotein-ubiquinone oxidoreductase YP_933655.1 2354316 D 62928 CDS YP_933656.1 119898443 4608676 complement(2356040..2357077) 1 NC_008702.1 Hypothetical secreted protein. Homology to GSU2347 of G. sulfurreducens of 26% (tremblnew|AAR35721(SRS)). Has PF04338;Protein of unknown function, DUF481; This family includes several proteins of uncharacterised function. Signal P reporting signal peptide present. NO TMH reported to be present.; hypothetical protein 2357077 4608676 azo2152 Azoarcus sp. BH72 hypothetical protein YP_933656.1 2356040 R 62928 CDS YP_933657.1 119898444 4607256 complement(2357201..2357677) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2380 of P. putida of 47% (trembl:Q88KB0). Has PF04314, Protein of unknown function (DUF461);Putative membrane or periplasmic protein. Signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 2357677 4607256 azo2153 Azoarcus sp. BH72 hypothetical protein YP_933657.1 2357201 R 62928 CDS YP_933658.1 119898445 4607257 complement(2357725..2358195) 1 NC_008702.1 Conserved hypothetical secreted protein. 33% similarity to TrEMBL Q8XQF8 PROBABLE SIGNAL PEPTIDE PROTEIN [RS05314] [Ralstonia solanacearum (Pseudomonas solanacearum)]. No domains predicted. Signal P reporting signal peptide present. No TMH reported being present.; Conserved hypothetical protein; hypothetical protein 2358195 4607257 azo2154 Azoarcus sp. BH72 hypothetical protein YP_933658.1 2357725 R 62928 CDS YP_933659.1 119898446 4607258 complement(2358192..2359436) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc1270 of R. solanacearum of 44% (trembl|Q8XQF7(SRS)). no domains predicted .signal peptide present. no TMHs; Conserved hypothetical protein; hypothetical protein 2359436 4607258 azo2155 Azoarcus sp. BH72 hypothetical protein YP_933659.1 2358192 R 62928 CDS YP_933660.1 119898447 4607259 complement(2359411..2361702) 1 NC_008702.1 TonB-dependent outer membrane receptor involved in the first step of iron internalization and metabolism. A short conserved region so called the tonB-box is present in the sequence. This domain is involved in the interaction of the receptor with the TonB protein. Similar to a E. coli iron(III) dicitrate transport protein fecA precursor. InterPro: TonB-dependent receptor protein arcC: carbamate kinase; Function unclear; TonB-dependent receptor 2361702 4607259 azo2156 Azoarcus sp. BH72 TonB-dependent receptor YP_933660.1 2359411 R 62928 CDS YP_933661.1 119898448 4607260 complement(2361956..2362348) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BB1293 of Bordetella bronchiseptica of 38% (trembl:Q7WMU7). No domains predicted. Signal peptide. No TMHs; hypothetical protein 2362348 4607260 azo2157 Azoarcus sp. BH72 hypothetical protein YP_933661.1 2361956 R 62928 CDS YP_933662.1 119898449 4607261 complement(2362426..2366367) 1 NC_008702.1 catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase 2366367 purL 4607261 purL Azoarcus sp. BH72 phosphoribosylformylglycinamidine synthase YP_933662.1 2362426 R 62928 CDS YP_933663.1 119898450 4609662 2366563..2367378 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV3583 of C. violaceum of 30% (trembl|Q7NS44(SRS)). Pfam: PAP2 superfamily no signal peptide 8 TMHs; Conserved hypothetical protein; hypothetical protein 2367378 4609662 azo2159 Azoarcus sp. BH72 hypothetical protein YP_933663.1 2366563 D 62928 CDS YP_933664.1 119898451 4607262 2367375..2368289 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to smc02385 of S. meliloti of 32% (trembl:Q92KI4). No domains predicted. Signal peptide. 6 TMHs; Conserved hypothetical protein; hypothetical protein 2368289 4607262 azo2160 Azoarcus sp. BH72 hypothetical protein YP_933664.1 2367375 D 62928 CDS YP_933665.1 119898452 4607263 2368307..2369353 1 NC_008702.1 Glycosyltransferase,; Specificity unclear; glycosyltransferase 2369353 4607263 azo2161 Azoarcus sp. BH72 glycosyltransferase YP_933665.1 2368307 D 62928 CDS YP_933666.1 119898453 4607264 2369350..2370978 1 NC_008702.1 Hypothetical protein AQ_1704.; Family membership; glycosyltransferases 2370978 4607264 azo2162 Azoarcus sp. BH72 glycosyltransferases YP_933666.1 2369350 D 62928 CDS YP_933667.1 119898454 4607265 2370975..2371727 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03001595 of Dechloromonas aromatica of 38% (gi|53730877|ref|ZP_00348993.1|(NBCI ENTREZ)). InterPro IPR008934; AcPase_VanPerase. IPR000326; PA_PTPase. Pfam PF01569; PAP2; 1. Number of predicted TMHs: 6 Prediction: Non-secretory protein Signal peptide probability: 0.023; Conserved hypothetical protein; hypothetical protein 2371727 4607265 azo2163 Azoarcus sp. BH72 hypothetical protein YP_933667.1 2370975 D 62928 CDS YP_933668.1 119898455 4607266 complement(2371731..2372156) 1 NC_008702.1 Function unclear; hypothetical protein 2372156 4607266 azo2164 Azoarcus sp. BH72 hypothetical protein YP_933668.1 2371731 R 62928 CDS YP_933669.1 119898456 4607267 complement(2372204..2372563) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA6137 of Azoarcus sp. EbN1 of 34% (gnl|keqq|eba:ebA6137(KEGG)). No domains predicted. Signal peptide present. No TMHs Coiled Coil present.; Conserved hypothetical protein; hypothetical protein 2372563 4607267 azo2165 Azoarcus sp. BH72 hypothetical protein YP_933669.1 2372204 R 62928 CDS YP_933670.1 119898457 4607268 complement(2372572..2373516) 1 NC_008702.1 InterPro: Peptidase family M23/M37; Specificity unclear; peptidase 2373516 4607268 azo2166 Azoarcus sp. BH72 peptidase YP_933670.1 2372572 R 62928 CDS YP_933671.1 119898458 4607269 complement(2373743..2376109) 1 NC_008702.1 catalyzes the formation of phosphoenolpyruvate from pyruvate; phosphoenolpyruvate synthase 2376109 ppsA1 4607269 ppsA1 Azoarcus sp. BH72 phosphoenolpyruvate synthase YP_933671.1 2373743 R 62928 CDS YP_933672.1 119898459 4609615 2376269..2377102 1 NC_008702.1 Conserved hypothetical protein. Homolog to RSC1420 of Ralstonia solanacearum of 63% (trembl:Q8XZH5). Has PF03618, Domain of unknown function (DUF299); IPR005177 ; Family of bacterial proteins with no known function. No signal peptide. No TMHs.; hypothetical protein 2377102 4609615 azo2168 Azoarcus sp. BH72 hypothetical protein YP_933672.1 2376269 D 62928 CDS YP_933673.1 119898460 4607270 2377163..2378341 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to BPP2392 of B.parapertussis of 42% (trembl:Q7W7W7). Signal P reporting signal peptide present. No TMH present. No domains predicted; Family membership; hypothetical protein 2378341 4607270 azo2169 Azoarcus sp. BH72 hypothetical protein YP_933673.1 2377163 D 62928 CDS YP_933674.1 119898461 4607271 2378368..2378784 1 NC_008702.1 Conserved hypothetical peptide methionine sulfoxide reductase. Homology to msrB of S. elongatus of 64%. Peptide methionine sulphoxide reductase reverses the inactivation of many proteins due to the oxidation of critical methionine residues by reducing methionine sulphoxide, Met(O), to methionine. InterPro: Domain of unknown function DUF25 (IPR002579). Pfam: Domain of unknown function DUF25. Tigrfam: TIGR00357: PilB-related protein. no TMHs; Family membership; peptide methionine sulfoxide reductase 2378784 msrB 4607271 msrB Azoarcus sp. BH72 peptide methionine sulfoxide reductase YP_933674.1 2378368 D 62928 CDS YP_933675.1 119898462 4609281 2378890..2380518 1 NC_008702.1 The Trg transducer mediates chemotactic response to galactose and ribose by interacting, respectively, with sugar-occupied galactose- and ribose-binding proteins. Adaptation is linked to methylation of specific glutamyl residues of the Trg protein. Similar to sprot|MCP3_ECOLI (27%)and to TREMBL:Q88AC6. InterPro (IPR004089): Bacterial chemotaxis sensory transducer InterPro (IPR003660): HAMP InterPro (IPR004090): Methyl-accepting chemotaxis protein Pfam (PF00672): HAMP Pfam (PF00015): Methyl-accepting chemotaxis protein (MCP) signaling domain Pfam (PF05227): CHASE3 domain TMHMM reporting two TMH.; Specificity unclear; putative ribose and galactose chemoreceptor protein 2380518 trg 4609281 trg Azoarcus sp. BH72 putative ribose and galactose chemoreceptor protein YP_933675.1 2378890 D 62928 CDS YP_933676.1 119898463 4609968 complement(2380606..2381787) 1 NC_008702.1 catalyzes the formation of beta-ketovaleryl-CoA from acetyl-CoA and propionyl-CoA; beta-ketothiolase 2381787 thlA 4609968 thlA Azoarcus sp. BH72 beta-ketothiolase YP_933676.1 2380606 R 62928 CDS YP_933677.1 119898464 4609940 complement(2381808..2382734) 1 NC_008702.1 catalyzes the formation of adenosylcobinamide-phosphate from adenosylcobyric acid; CobD/CbiB family protein 2382734 cobD1 4609940 cobD1 Azoarcus sp. BH72 CobD/CbiB family protein YP_933677.1 2381808 R 62928 CDS YP_933678.1 119898465 4608501 complement(2382839..2383312) 1 NC_008702.1 Region start changed from 2383430 to 2383313 (-117 bases); hypothetical protein 2383312 4608501 azo2174 Azoarcus sp. BH72 hypothetical protein YP_933678.1 2382839 R 62928 CDS YP_933679.1 119898466 4607272 complement(2383583..2386957) 1 NC_008702.1 PilY1 is a large protein with C-terminal homology to the PilC2 protein of Neisseria gonorrhoeae, thought to be a fimbrial tip-associated adhesin, and which, like PilY1, is involved in fimbrial assembly. Similar to trembl|Q8XW00 (31%). Pfam (PF01839): FG-GAP repeat SignalP reporting Signal peptide; Function unclear; putative type 4 pilus biogenesis protein 2386957 pilY1A 4607272 pilY1A Azoarcus sp. BH72 putative type 4 pilus biogenesis protein YP_933679.1 2383583 R 62928 CDS YP_933680.1 119898467 4609579 complement(2386969..2387559) 1 NC_008702.1 Hypothetical secreted protein. Homology to RS00045 of R. solanacearum of 25% (trembl|Q8XVZ9(SRS)) No domains predicted Sinal P reporting singal peptide present. No TMH being reported.; hypothetical protein 2387559 4609579 azo2176 Azoarcus sp. BH72 hypothetical protein YP_933680.1 2386969 R 62928 CDS YP_933681.1 119898468 4607273 complement(2387559..2388581) 1 NC_008702.1 In Pseudomonas aeruginosa, the PilW and PilX proteins are membrane located, possess the hydrophobic N-terminus characteristic of prepilin-like proteins, and appear to belong to the GspJ and GspK group of proteins that are required for protein secretion in a wide range of Gram-negative bacteria. These findings increase the similarities between the fimbrial biogenesis and the Gsp-based protein-secretion supersystems. Similar to trembl|Q8XVZ8 (29%). Smart (SM00438): Repressor of transcription TMHMM reporting one TMH. SignalP reporting Signal peptide.; Function unclear; putative type 4 pilus biogenesis protein 2388581 pilW 4607273 pilW Azoarcus sp. BH72 putative type 4 pilus biogenesis protein YP_933681.1 2387559 R 62928 CDS YP_933682.1 119898469 4609577 complement(2388592..2389143) 1 NC_008702.1 Family membership; type 4 fimbrial biogenesis relatedtransmembrane protein 2389143 4609577 azo2178 Azoarcus sp. BH72 type 4 fimbrial biogenesis relatedtransmembrane protein YP_933682.1 2388592 R 62928 CDS YP_933683.1 119898470 4607274 complement(2389188..2389595) 1 NC_008702.1 Probable type-4 fimbrial pilin related signal peptide protein,32% Identity to TrEMBL;Q8XVZ6,Q7NTE6. Has Signal peptide. Has COG4970, FimT, Tfp pilus assembly protein FimT [Cell motility and secretion / Intracellular trafficking and secretion].; hypothetical protein 2389595 4607274 azo2179 Azoarcus sp. BH72 hypothetical protein YP_933683.1 2389188 R 62928 CDS YP_933684.1 119898471 4607275 complement(2389690..2390103) 1 NC_008702.1 In P. aeruginosa PilE is probable involved in pilus biogenesis and in twitching motility. Similarity to PilE of Ralstonia eutropha (33%) TMHMM reporting one TMhelix. Pfam: pilin domain; Conserved hypothetical protein; hypothetical protein 2390103 4607275 azo2180 Azoarcus sp. BH72 hypothetical protein YP_933684.1 2389690 R 62928 CDS YP_933685.1 119898472 4607276 complement(2390211..2390861) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pa4048 of P. aeruginosa of 33% (trembl|Q9HWY0). no domains. signal peptide. no TMHs.; Conserved hypothetical protein; hypothetical protein 2390861 4607276 azo2181 Azoarcus sp. BH72 hypothetical protein YP_933685.1 2390211 R 62928 CDS YP_933686.1 119898473 4607277 complement(2390978..2391172) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA5103 of Azoarcus sp. EbN1 of 57% (gnl|keqq|eba:ebA5103(KEGG)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 2391172 4607277 azo2182 Azoarcus sp. BH72 hypothetical protein YP_933686.1 2390978 R 62928 CDS YP_933687.1 119898474 4607278 complement(2391193..2391678) 1 NC_008702.1 putative nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription; putative nucleotide-binding protein 2391678 4607278 azo2183 Azoarcus sp. BH72 putative nucleotide-binding protein YP_933687.1 2391193 R 62928 CDS YP_933688.1 119898475 4607279 complement(2391697..2392020) 1 NC_008702.1 Hypothetical protein,64% identical to TrEMBL;Q8XWC2 Has PF06865, Protein of unknown function (DUF1255);IPR009664 :This family consists of several conserved hypothetical bacterial proteins of around 95 residues in length. The function of this family is unknown. No Signal peptide or TMH not present.; High confidence in function and specificity; hypothetical protein 2392020 yaiE 4607279 yaiE Azoarcus sp. BH72 hypothetical protein YP_933688.1 2391697 R 62928 CDS YP_933689.1 119898476 4610139 complement(2392036..2392263) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NMA1807 of Neisseria meningitidis of 41% (trembl|Q9JTE8(SRS)). No domains predicted. TMHMM reporting 2 TMH present. NO Signal Peptide being reported present.; Conserved hypothetical protein; hypothetical protein 2392263 4610139 azo2185 Azoarcus sp. BH72 hypothetical protein YP_933689.1 2392036 R 62928 CDS YP_933690.1 119898477 4607280 complement(2392276..2393505) 1 NC_008702.1 catalyzes the formation of 2-N(omega)-(L-arginino)succinate from L-citrulline and L-aspartate in arginine biosynthesis, AMP-forming; argininosuccinate synthase 2393505 argG 4607280 argG Azoarcus sp. BH72 argininosuccinate synthase YP_933690.1 2392276 R 62928 CDS YP_933691.1 119898478 4608336 complement(2393568..2394503) 1 NC_008702.1 catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation; ornithine carbamoyltransferase 2394503 argF 4608336 argF Azoarcus sp. BH72 ornithine carbamoyltransferase YP_933691.1 2393568 R 62928 CDS YP_933692.1 119898479 4608335 complement(2394503..2395693) 1 NC_008702.1 catalyzes the formation of N-acetyl-l-glutamate 5-semialdehyde from 2-oxoglutarate and N(2)-acetyl-L-ornithine; acetylornithine aminotransferase 2395693 argD 4608335 argD Azoarcus sp. BH72 acetylornithine aminotransferase YP_933692.1 2394503 R 62928 CDS YP_933693.1 119898480 4608334 2395933..2396217 1 NC_008702.1 Conserved hypothetical protein. Homology to bpp2544 of P. pararpertussis of 51% (TREMBL:Q7W7H5). no signal peptide. no TMHs. No domains predicted.; hypothetical protein 2396217 4608334 azo2189 Azoarcus sp. BH72 hypothetical protein YP_933693.1 2395933 D 62928 CDS YP_933694.1 119898481 4607281 complement(2396348..2396614) 1 NC_008702.1 binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase; 30S ribosomal protein S20 2396614 rpsT 4607281 rpsT Azoarcus sp. BH72 30S ribosomal protein S20 YP_933694.1 2396348 R 62928 CDS YP_933695.1 119898482 4609823 2396768..2398306 1 NC_008702.1 TREMBL:Q82SD5:58% identity, 69% similarity The sequencing of a number of pathogenic bacterial genomes has led to novel virulence proteins being discovered that are yet to be biochemically characterised. One example is the MviN family of proteins, first described in Salmonella [1], and conserved across a wide variety of pathogens in both animals and plants. Further work on these proteins of as yet unknown function has revealed they are integral membrane molecules, and are part of an operon essential in at least one species subcellular location:integral membrane protein (potential). similarity:belongs to the mvin family Virulence factor mviN. InterPro:IPR004268; MVIN_like. Pfam:PF03023; MVIN (Virulence factor MVIN) InterPro: Virulence factor MVIN-like TMHMM predicted 13 transmembrane helices matE: MATE efflux family protein; High confidence in function and specificity; virulence factor 2398306 mviN 4609823 mviN Azoarcus sp. BH72 virulence factor YP_933695.1 2396768 D 62928 CDS YP_933696.1 119898483 4609300 complement(2398320..2398643) 1 NC_008702.1 Hypothetical protein, has very bad homology with hits in the database. TrEMBL;Q762H6. Has Signal Peptide.; hypothetical protein 2398643 4609300 azo2192 Azoarcus sp. BH72 hypothetical protein YP_933696.1 2398320 R 62928 CDS YP_933697.1 119898484 4607282 2398921..2400192 1 NC_008702.1 Probable O-acetylhomoserine aminocarboxypropyltransferase. Homology to cysD of A. nidulans of 51% (sprot|CYSD_EMENI). Transforms O-acetylhomoserine into homocysteine. Pfam: Cys/Met metabolism PLP-dependent enzyme no signal peptide no TMHs; High confidence in function and specificity; O-acetylhomoserine aminocarboxypropyltransferase 2400192 4607282 azo2193 Azoarcus sp. BH72 O-acetylhomoserine aminocarboxypropyltransferase YP_933697.1 2398921 D 62928 CDS YP_933698.1 119898485 4607283 complement(2400229..2403828) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs04519 of R. solanacearum of 33% (trembl|Q8Y0Z7(SRS)). no domains predicted. no signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 2403828 4607283 azo2194 Azoarcus sp. BH72 hypothetical protein YP_933698.1 2400229 R 62928 CDS YP_933699.1 119898486 4607284 2403971..2405179 1 NC_008702.1 Hypothetical protein yhiN. FAD flavoproteins belong to the family of pyridine nucleotide-disulphide oxidoreductases TREMBL:Q7MBF1: 58% identity, 69% similarity InterPro: IPR001327; FAD_pyr_redox. IPR004792; HI0933_like. IPR000205; NAD_BS. IPR001100; Pyr_redox InterPro: HI0933-like protein Pfam:PF03486; HI0933_like; 1. TIGR00275: conserved hypothetical pro Non-secretory protein with signal peptide probability of 0.464 Absence of transmembrane helices (TMHMM predicted); Specificity unclear; flavoprotein 2405179 4607284 azo2195 Azoarcus sp. BH72 flavoprotein YP_933699.1 2403971 D 62928 CDS YP_933700.1 119898487 4607285 2405255..2406064 1 NC_008702.1 Metallo-phosphoesterase, 50% identity(67% similarity) to TrEMBL;Q6N2S9 . TrEMBL;Q885N2(53% identity). Has PF00149, Calcineurin-like phosphoesterase;IPR004843,M-pesterase:This family includes a diverse range of phosphoesterases, including protein phosphoserine phosphatases, nucleotidases, sphingomyelin phosphodiesterases and 2'-3' cAMP phosphodiesterases as well as nucleases such as bacterial SbcD P13457 or yeast MRE11 P32829. The most conserved regions in this superfamily centre around the metal chelating residues. The metallo-phosphoesterase motif is found in a large number of proteins invoved in phosphoryation. These include serine/threonine phosphatases, DNA polymerase,exonucleases, and other phosphatases. No Signal Peptide or TMH present.; Specificity unclear; metallo-phosphoesterase 2406064 4607285 azo2196 Azoarcus sp. BH72 metallo-phosphoesterase YP_933700.1 2405255 D 62928 CDS YP_933701.1 119898488 4607286 2406216..2406671 1 NC_008702.1 Putative bacterioferritin comigratory protein. Homology to bcp of E. coli of 37% (sprot|BCP_ECOLI) InterPro: Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (IPR000866) Pfam: AhpC/TSA family no signal peptide no TMHs; Family membership; putative bacterioferritin comigratory protein 2406671 bcp2 4607286 bcp2 Azoarcus sp. BH72 putative bacterioferritin comigratory protein YP_933701.1 2406216 D 62928 CDS YP_933702.1 119898489 4608380 2406695..2408152 1 NC_008702.1 Putative PhoH-related protein,; Conserved hypothetical protein; putative PhoH-related protein 2408152 4608380 azo2198 Azoarcus sp. BH72 putative PhoH-related protein YP_933702.1 2406695 D 62928 CDS YP_933703.1 119898490 4607287 2408503..2410050 1 NC_008702.1 Probable aerotaxis receptor protein,; High confidence in function and specificity; aerotaxis receptor 2410050 aer 4607287 aer Azoarcus sp. BH72 aerotaxis receptor YP_933703.1 2408503 D 62928 CDS YP_933704.1 119898491 4608283 2410091..2411239 1 NC_008702.1 HD-domain containing protein,; Conserved hypothetical protein; HD-domain-containing protein 2411239 4608283 azo2200 Azoarcus sp. BH72 HD-domain-containing protein YP_933704.1 2410091 D 62928 CDS YP_933705.1 119898492 4607288 2411332..2413230 1 NC_008702.1 Putative nucleotidyltransferase,; Conserved hypothetical protein; putative nucleotidyltransferase 2413230 4607288 azo2201 Azoarcus sp. BH72 putative nucleotidyltransferase YP_933705.1 2411332 D 62928 CDS YP_933706.1 119898493 4607289 2413227..2413871 1 NC_008702.1 Probable DNA polymerase III, epsilon subunit,23% Identity to TrEMBL;Q8KDK8. Has EXOIII,SMART;SM00479;IPR006054, DnaQ;This family includes a variety of exonucleases, such as ribonuclease T and the epsilon subunit of DNA polymerase III. Ribonuclease T is responsible for the end-turnover of tRNA,and removes the terminal AMP residue from uncharged tRNA. DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria, and also exhibits 3' to 5' exonuclease activity.; DNA-directed DNA polymerase 2413871 4607289 azo2202 Azoarcus sp. BH72 DNA-directed DNA polymerase YP_933706.1 2413227 D 62928 CDS YP_933707.1 119898494 4607290 2413954..2414493 1 NC_008702.1 InterPro: NLP/P60; Family membership; putative lipoprotein precusor 2414493 4607290 azo2203 Azoarcus sp. BH72 putative lipoprotein precusor YP_933707.1 2413954 D 62928 CDS YP_933708.1 119898495 4607291 complement(2414546..2414887) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2970 of C.violaceum of 42% (trembl:Q7NTT3). No domains predicted. No TMHs. No signal peptide.; Function unclear; hypothetical protein 2414887 4607291 azo2204 Azoarcus sp. BH72 hypothetical protein YP_933708.1 2414546 R 62928 CDS YP_933709.1 119898496 4607292 2415014..2416036 1 NC_008702.1 Iron (III)-transport system, substrate-binding protein FbpA. Part of the ABC transporter complex fbpABC (TC 3.A.1.10.1) involved in ferric cations import.Iron(III)periplasmic binding protein component.; High confidence in function and specificity; putative iron transport system substrate-binding protein 2416036 fbpA1 4607292 fbpA1 Azoarcus sp. BH72 putative iron transport system substrate-binding protein YP_933709.1 2415014 D 62928 CDS YP_933710.1 119898497 4608730 complement(2416091..2417758) 1 NC_008702.1 Iron(III)-transport system permease fbpB 2. Part of the ABC transporter complex fbpABC (TC 3.A.1.10.1) involved in ferric cations import. Probably responsible for the translocation of the substrate across the membrane (By similarity). pts-Glc: PTS system maltose and gluco; High confidence in function and specificity; iron (III) transport system permease 2417758 fbpB1 4608730 fbpB1 Azoarcus sp. BH72 iron (III) transport system permease YP_933710.1 2416091 R 62928 CDS YP_933711.1 119898498 4608732 2417875..2418999 1 NC_008702.1 Ferric cations import ATP-binding protein fbpC 2 (EC 3.6.3.30). Part of the ABC transporter complex fbpABC (TC 3.A.1.10.1) involved in ferric cations import. Responsible for energy coupling to the transport system (By similarity). InterPro: AAA ATPase superfamily apsK: adenylylsulfate kinase; Specificity unclear; putative iron (III) transport system ATP-binding protein 2418999 fbpC1 4608732 fbpC1 Azoarcus sp. BH72 putative iron (III) transport system ATP-binding protein YP_933711.1 2417875 D 62928 CDS YP_933712.1 119898499 4608733 2418999..2419706 1 NC_008702.1 leucyltransferase; phenylalanyltransferse; functions in the N-end rule pathway; transfers Leu, Phe, Met, from aminoacyl-tRNAs to N-terminal of proteins with Arg or Lys; leucyl/phenylalanyl-tRNA--protein transferase 2419706 aat 4608733 aat Azoarcus sp. BH72 leucyl/phenylalanyl-tRNA--protein transferase YP_933712.1 2418999 D 62928 CDS YP_933713.1 119898500 4608244 2419667..2420506 1 NC_008702.1 Conjugates Arg from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate; arginyl-tRNA-protein transferase 2420506 ate 4608244 ate Azoarcus sp. BH72 arginyl-tRNA-protein transferase YP_933713.1 2419667 D 62928 CDS YP_933714.1 119898501 4608363 2420577..2421593 1 NC_008702.1 catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors; dihydroorotate dehydrogenase 2 2421593 pyrD 4608363 pyrD Azoarcus sp. BH72 dihydroorotate dehydrogenase 2 YP_933714.1 2420577 D 62928 CDS YP_933715.1 119898502 4609670 2421804..2423330 1 NC_008702.1 Xanthine dehydrogenase (subunit A) oxidoreductase protein; High confidence in function and specificity; xanthine dehydrogenase 2423330 xdhA 4609670 xdhA Azoarcus sp. BH72 xanthine dehydrogenase YP_933715.1 2421804 D 62928 CDS YP_933716.1 119898503 4610124 2423317..2425656 1 NC_008702.1 Xanthine dehydrogenase (subunit B) InterPro: Aldehyde oxidase and xanthine dehydrogenase C terminus; High confidence in function and specificity; xanthine dehydrogenase 2425656 xdhB 4610124 xdhB Azoarcus sp. BH72 xanthine dehydrogenase YP_933716.1 2423317 D 62928 CDS YP_933717.1 119898504 4610125 2425667..2426761 1 NC_008702.1 xanthine dehydrogenase, probable (chaperone); High confidence in function and specificity; putative xanthine dehydrogenase 2426761 xdhC 4610125 xdhC Azoarcus sp. BH72 putative xanthine dehydrogenase YP_933717.1 2425667 D 62928 CDS YP_933718.1 119898505 4610126 2426758..2428323 1 NC_008702.1 Conserved hypothetical sugar transporter ATP-binding protein. Homology to orf96 of P. sp strain ADP of 65% (TREMBL:Q936X7). PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR sugars. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. Pfam: AAA-ATPase; ABC transporter. no signal peptide. no TMHs; Specificity unclear; hypothetical protein 2428323 4610126 azo2214 Azoarcus sp. BH72 hypothetical protein YP_933718.1 2426758 D 62928 CDS YP_933719.1 119898506 4607293 2428310..2429374 1 NC_008702.1 Conserved hypothetical ABC transporter permease. Homology to orf95 of Pseudomonas sp strain ADP of 57% (trembl|Q936X8). Bacterial binding protein-dependent transport systems are multicomponent systems typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system. InterPro: Binding-system dependent bacterial transporters (araH livH/limM families) (IPR001851). Pfam: Branched-chain ammino acid transporter/permease component. no signal peptide. 9 TMHs; Specificity unclear; ABC transporter permease 2429374 4607293 azo2215 Azoarcus sp. BH72 ABC transporter permease YP_933719.1 2428310 D 62928 CDS YP_933720.1 119898507 4607294 2429377..2430309 1 NC_008702.1 TREMBL:Q8XXD9: 46% identity, 59% similarity Hypothetical protein MG120 homolog (A65_orf517). Bacterial inner-membrane translocator.The integral inner-membrane proteins translocate the substrate across the membrane. It has been shown [3, 4] that most of these proteins contain a conserved region located about 80 to 100 residues from their C-terminal extremity InterPro:IPR001851; Bac_inmem_transp. Pfam:PF02653; BPD_transp_2 gntP: gluconate transporter TMH's 9 (TMHMM predicted); Specificity unclear; ABC transporter permease 2430309 4607294 azo2216 Azoarcus sp. BH72 ABC transporter permease YP_933720.1 2429377 D 62928 CDS YP_933721.1 119898508 4607295 2430582..2432618 1 NC_008702.1 Similar to FecA, ferric-citrate outer membrane receptor in E.coli, involved in the recognition and internalization of ferric-citrate. InterPro: TonB-dependent receptor protein; Function unclear; putative TonB-dependent receptor 2432618 4607295 azo2217 Azoarcus sp. BH72 putative TonB-dependent receptor YP_933721.1 2430582 D 62928 CDS YP_933722.1 119898509 4607296 2432622..2433353 1 NC_008702.1 Part of the ABC transporter complex cbiKMQO involved in cobalt import. Similar to the putative periplasmic-binding protein CbiK precursor in A.pleuropneumoniae. thiE: thiamine-phosphate pyrophosphoryla; High confidence in function and specificity; putative cobalt transport system periplasmic-binding protein 2433353 cbiK 4607296 cbiK Azoarcus sp. BH72 putative cobalt transport system periplasmic-binding protein YP_933722.1 2432622 D 62928 CDS YP_933723.1 119898510 4608430 2433350..2433829 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cbiL of Actinobacillus pleuropneumoniae of 39% (gi|5733705|gb|AAD49725.1|(NBCI ENTREZ)). Signal P reproting signal peptide present. TMHMM reporting TMH present.; Conserved hypothetical protein; hypothetical protein 2433829 4608430 azo2219 Azoarcus sp. BH72 hypothetical protein YP_933723.1 2433350 D 62928 CDS YP_933724.1 119898511 4607297 2433832..2434458 1 NC_008702.1 catalyzes the ATP-dependent transport of cobalt; cobalt transport protein CbiM 2434458 cbiM 4607297 cbiM Azoarcus sp. BH72 cobalt transport protein CbiM YP_933724.1 2433832 D 62928 CDS YP_933725.1 119898512 4608432 2434455..2435144 1 NC_008702.1 Part of the ABC transporter complex cbiKMQO involved in cobalt import. Similar to the cobalt transport membrane protein, CbiQ in A. pleuropneumoniae.; Function unclear; putative cobalt transport system permease 2435144 cbiQ 4608432 cbiQ Azoarcus sp. BH72 putative cobalt transport system permease YP_933725.1 2434455 D 62928 CDS YP_933726.1 119898513 4608434 2435203..2435838 1 NC_008702.1 Part of the ABC transporter complex cbiKMQO involved in cobalt import. Probably responsible for the translocation of the substrate across the membrane. 40% AAA_ATPase.IPR003439; ABC_transporter. Pfam:PF00005; ABC_tran; 1. ProDom; PD000006; ABC_transporter; 1. SMART; SM00382; AAA; 1.; High confidence in function and specificity; putative cobalt transport system ATP-binding protein 2435838 cbiO 4608434 cbiO Azoarcus sp. BH72 putative cobalt transport system ATP-binding protein YP_933726.1 2435203 D 62928 CDS YP_933727.1 119898514 4608433 2435887..2437182 1 NC_008702.1 Catalyzes the deamination of guanine; guanine deaminase 2437182 guaD 4608433 guaD Azoarcus sp. BH72 guanine deaminase YP_933727.1 2435887 D 62928 CDS YP_933728.1 119898515 4608963 2437170..2437643 1 NC_008702.1 Hypothetical protein yaiI,58% identical (75% similar) to SwissProt; P52088, of E.coli.SwissProt;Q8XEM1(61% identity) TrEMBL; Q8ED72. Has PF02639, Uncharacterized BCR, YaiI/YqxD family COG1671;IPR003791. No Signal peptide or TMH present.; YaiI/YqxD family protein 2437643 yaiI 4608963 yaiI Azoarcus sp. BH72 YaiI/YqxD family protein YP_933728.1 2437170 D 62928 CDS YP_933729.1 119898516 4610140 2437796..2439499 1 NC_008702.1 GGDEF/EAL/PAS-domain containing protein; diguanylate cyclase 2439499 4610140 azo2225 Azoarcus sp. BH72 diguanylate cyclase YP_933729.1 2437796 D 62928 CDS YP_933730.1 119898517 4607298 complement(2439509..2440297) 1 NC_008702.1 Probable N-acetylmuramoyl-L-alanine amidase amiA precursor, 28% identity to TrEMBL;Q823N1. 26% identity to SwissProt;P36548 Has SMART;SM00646, Ami_3. Signal Peptide present. Has PF01520; N-acetylmuramoyl-L-alanine amidase ;IPR002508 Amidase_3_hydro; This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.; N-acetylmuramoyl-L-alanine amidase 2440297 4607298 azo2226 Azoarcus sp. BH72 N-acetylmuramoyl-L-alanine amidase YP_933730.1 2439509 R 62928 CDS YP_933731.1 119898518 4607299 complement(2440284..2442953) 1 NC_008702.1 GGDEF/EAL/PAS-domain containing protein; diguanylate cyclase 2442953 4607299 azo2227 Azoarcus sp. BH72 diguanylate cyclase YP_933731.1 2440284 R 62928 CDS YP_933732.1 119898519 4607300 complement(2442950..2443813) 1 NC_008702.1 Conserved hypothetical amino acid-binding protein. Homology to bb2160 of B. bronchiseptica of 39% (trembl|Q7WKE7). PROBABLY PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR AN AMINO ACID. InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR001311). Pfam: Bacterial extracellular solute-binding protein. Signal peptide. no TMHs; Family membership; amino acid-binding protein 2443813 4607300 azo2228 Azoarcus sp. BH72 amino acid-binding protein YP_933732.1 2442950 R 62928 CDS YP_933733.1 119898520 4607301 2443996..2444244 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PSPTO5169 of P.syringae 45% (tremble:Q87UX3). No domains predicted. Signal P reporting Signal Peptide present. No TMH being reported as present.; Conserved hypothetical protein; hypothetical protein 2444244 4607301 azo2229 Azoarcus sp. BH72 hypothetical protein YP_933733.1 2443996 D 62928 CDS YP_933734.1 119898521 4607302 2444384..2444647 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to pp2721 of P. putida of 56% (trembl|Q88JC8(SRS). no domains predicted. no signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 2444647 4607302 azo2230 Azoarcus sp. BH72 hypothetical protein YP_933734.1 2444384 D 62928 CDS YP_933735.1 119898522 4607303 complement(2444681..2445784) 1 NC_008702.1 Mannose-1-phosphate guanyltransferase involved in the reaction:GTP + alpha-D-mannose 1-phosphate = diphosphate + GDPmannose. 51% Hexapep_transf.IPR005835; NTP_transferase. Pfam:PF00132; Hexapep; 2.PF00483; NTP_transferase; 1.; High confidence in function and specificity; putative mannose-1-phosphate guanylyltransferase 2445784 4607303 azo2231 Azoarcus sp. BH72 putative mannose-1-phosphate guanylyltransferase YP_933735.1 2444681 R 62928 CDS YP_933736.1 119898523 4607304 complement(2445816..2447138) 1 NC_008702.1 61% UDPG_MGDP_dh. Pfam:PF00984; UDPG_MGDP_dh; 1.PF03720; UDPG_MGDP_dh_C; 1.PF03721; UDPG_MGDP_dh_N; 1. Signal peptide: present.; High confidence in function and specificity; putative UDP-glucose 6-dehydrogenase 2447138 udgH 4607304 udgH Azoarcus sp. BH72 putative UDP-glucose 6-dehydrogenase YP_933736.1 2445816 R 62928 CDS YP_933737.1 119898524 4610063 complement(2447180..2447938) 1 NC_008702.1 60% WecB_TagA_CpsF. Pfam:PF03808; Glyco_tran_WecB; 1. TIGRFAMs:TIGR00696; wecB_tagA_cpsF; 1. Non-secretory protein.; High confidence in function and specificity; putative glycosyltransferase 2447938 gumM 4610063 gumM Azoarcus sp. BH72 putative glycosyltransferase YP_933737.1 2447180 R 62928 CDS YP_933738.1 119898525 4608973 complement(2447961..2449433) 1 NC_008702.1 25% Polysacc_synt. Pfam:PF01943; Polysacc_synt; 1. TMhelix:14. Non-secretory protein.; Family membership; putative polysaccharide translocase 2449433 gumJ 4608973 gumJ Azoarcus sp. BH72 putative polysaccharide translocase YP_933738.1 2447961 R 62928 CDS YP_933739.1 119898526 4608971 complement(2449543..2450694) 1 NC_008702.1 64%; High confidence in function and specificity; putative glucuronyl transferase 2450694 gumK 4608971 gumK Azoarcus sp. BH72 putative glucuronyl transferase YP_933739.1 2449543 R 62928 CDS YP_933740.1 119898527 4608972 complement(2450726..2451748) 1 NC_008702.1 41% Glyco_hydro_5. Pfam:PF00150; Cellulase; 1. Signal peptide: present.; High confidence in function and specificity; putative endoglucanase 2451748 eglA 4608972 eglA Azoarcus sp. BH72 putative endoglucanase YP_933740.1 2450726 R 62928 CDS YP_933741.1 119898528 4608663 complement(2451745..2452860) 1 NC_008702.1 60% Glyco_trans_1. Pfam:PF00534; Glycos_transf_1; 1.; Specificity unclear; putative glycosyltransferase 2452860 gumH 4608663 gumH Azoarcus sp. BH72 putative glycosyltransferase YP_933741.1 2451745 R 62928 CDS YP_933742.1 119898529 4608970 complement(2452857..2454176) 1 NC_008702.1 26%; Family membership; putative surface polysaccharide polymerase 2454176 gumE 4608970 gumE Azoarcus sp. BH72 putative surface polysaccharide polymerase YP_933742.1 2452857 R 62928 CDS YP_933743.1 119898530 4608968 complement(2454178..2455266) 1 NC_008702.1 44% Acyl_transf_3. Pfam:PF01757; Acyl_transf_3; 1. TMhelix:9. Non-secretory protein.; High confidence in function and specificity; putative acetyltransferase 2455266 gumF 4608968 gumF Azoarcus sp. BH72 putative acetyltransferase YP_933743.1 2454178 R 62928 CDS YP_933744.1 119898531 4608969 complement(2455235..2456101) 1 NC_008702.1 Tyrosine-protein kinase wzc (EC 2.7.10.1). Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium (By similarity). gidA: glucose-inhibited division prot; High confidence in function and specificity; tyrosine-protein kinase 2456101 wzc1 4608969 wzc1 Azoarcus sp. BH72 tyrosine-protein kinase YP_933744.1 2455235 R 62928 CDS YP_933745.1 119898532 4610118 complement(2456144..2457553) 1 NC_008702.1 21% LPS_Wzz_MPA. Pfam:PF02706; Wzz; 1. TMhelix:1. Signal peptide:present.; High confidence in function and specificity; putative exopolysaccharide biosynthesis protein 2457553 gumC 4610118 gumC Azoarcus sp. BH72 putative exopolysaccharide biosynthesis protein YP_933745.1 2456144 R 62928 CDS YP_933746.1 119898533 4608966 complement(2457567..2458376) 1 NC_008702.1 Putative capsule polysaccharide export protein precursor.May be involved in polysaccharide polymerization or transport. 36% Poly_export. Pfam:PF02563; Poly_export; 1. Signal peptide: present.; Family membership; putative polysaccharide export protein 2458376 gumB 4608966 gumB Azoarcus sp. BH72 putative polysaccharide export protein YP_933746.1 2457567 R 62928 CDS YP_933747.1 119898534 4608965 complement(2458429..2459328) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to TdenA01000001 of Thiobacillus denitrificans of 33% (gi|52007706|ref|ZP_00335083.1|(NBCI ENTREZ)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2459328 4608965 azo2243 Azoarcus sp. BH72 hypothetical protein YP_933747.1 2458429 R 62928 CDS YP_933748.1 119898535 4607305 complement(2459330..2459746) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains pedicted. No TMHs signal peptide; hypothetical protein 2459746 4607305 azo2244 Azoarcus sp. BH72 hypothetical protein YP_933748.1 2459330 R 62928 CDS YP_933749.1 119898536 4607306 complement(2459794..2460981) 1 NC_008702.1 Hypothetical secreted protein. No good homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 2460981 4607306 azo2245 Azoarcus sp. BH72 hypothetical protein YP_933749.1 2459794 R 62928 CDS YP_933750.1 119898537 4607307 2461174..2462565 1 NC_008702.1 43% Bact_transf. Pfam:PF02397; Bac_transf; 1. TMhelix:5.; Specificity unclear; putative glycosyltransferase 2462565 gumD 4607307 gumD Azoarcus sp. BH72 putative glycosyltransferase YP_933750.1 2461174 D 62928 CDS YP_933751.1 119898538 4608967 2462824..2464230 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 2464230 4608967 azo2247 Azoarcus sp. BH72 hypothetical protein YP_933751.1 2462824 D 62928 CDS YP_933752.1 119898539 4607308 complement(2464264..2464395) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2464395 4607308 azo2248 Azoarcus sp. BH72 hypothetical protein YP_933752.1 2464264 R 62928 CDS YP_933753.1 119898540 4607309 2464506..2465156 1 NC_008702.1 Region start changed from 2464498 to 2464507 (-9 bases); putative DNA polymerase-like protein 2465156 4607309 azo2249 Azoarcus sp. BH72 putative DNA polymerase-like protein YP_933753.1 2464506 D 62928 CDS YP_933754.1 119898541 4607310 complement(2465256..2466758) 1 NC_008702.1 probable succinate semialdehyde dehydrogenase [NAD(P)+). Homology to thmS of Pseudonocardia sp. K1 of 55% (trembl|Q9F3V7). Is capable of oxidizing substrates using NADP as cofactor. InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; Family membership; succinate semialdehyde dehydrogenase 2466758 thmS1 4607310 thmS1 Azoarcus sp. BH72 succinate semialdehyde dehydrogenase YP_933754.1 2465256 R 62928 CDS YP_933755.1 119898542 4609941 complement(2467221..2467694) 1 NC_008702.1 Hypothetical protein, 30% identiy(50% similarity) to TrEMBL;Q8FYJ1. TrEMBL;Q8YJA6. Has (IPR010755)PF07049,Protein of unknown function (DUF1332);This family consists of several hypothetical bacterial proteins of around 165 residues in length. The function of this family is unknown. No Signal peptide or TMH present.; Function unclear; hypothetical protein 2467694 4609941 azo2251 Azoarcus sp. BH72 hypothetical protein YP_933755.1 2467221 R 62928 CDS YP_933756.1 119898543 4607311 complement(2467705..2468685) 1 NC_008702.1 Conserved hypothetical BNR domain protein. Homology to pa3080 of P. aeruginosa of 38% (trembl|Q9HZC8). InterPro: BNR repeat (IPR002860). Pfam: BNR repeat. BNR repeats are short repeats never found closer than 40 residues together, which suggests that the repeat is structurally longer. These repeats are found in many glycosyl hydrolases as well as other extracellular proteins of unknown function. singal peptide. no TMHs; Function unclear; hypothetical protein 2468685 4607311 azo2252 Azoarcus sp. BH72 hypothetical protein YP_933756.1 2467705 R 62928 CDS YP_933757.1 119898544 4607312 complement(2468697..2471060) 1 NC_008702.1 Putative membrane protein MJ1562. TREMBL:Q88J37: 52% identity, 66% similarity InterPro:IPR001036; Acrflvin_res. IPR000731; SSD_5TM Pfam:Patched:Patched family 2A067: efflux transporter putative Presence of signal peptide (Signal P predicted). Transmembrane helices 12 (TMHMM predicted); Family membership; RND efflux transporter 2471060 4607312 azo2253 Azoarcus sp. BH72 RND efflux transporter YP_933757.1 2468697 R 62928 CDS YP_933758.1 119898545 4607313 complement(2471191..2472987) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2811 of P.putida of 32% (tremble:Q88J38). Has PF06980;Protein of unknown function (DUF1302)domain;IPR010727 This family contains a number of hypothetical bacterial proteins of unknown function that are approximately 600 residues long. Most family members seem to be from Pseudomonas. Signal peptide present. No TMH reported.; Conserved hypothetical protein; hypothetical protein 2472987 4607313 azo2254 Azoarcus sp. BH72 hypothetical protein YP_933758.1 2471191 R 62928 CDS YP_933759.1 119898546 4607314 complement(2473019..2474398) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA3081 of P.aeruginosa of 43% (trembl:Q9HZC7). Has pfam07044, DUF1329, Protein of unknown function (DUF1329). IPR010752; This family consists of several hypothetical bacterial proteins of around 475 residues in length. The majority of family members are from Pseudomonas species but the family also contains sequences from Shewanella oneidensis and Thauera aromatica. Signal Peptide present. No TMH being reported.; Conserved hypothetical protein; hypothetical protein 2474398 4607314 azo2255 Azoarcus sp. BH72 hypothetical protein YP_933759.1 2473019 R 62928 CDS YP_933760.1 119898547 4607315 complement(2474463..2474945) 1 NC_008702.1 Conserved hypothetical cytochrome c-552. Homology to Bcepa03004834 of Burkholderia cepacia of 38% (gi|46313246|ref|ZP_00213837.1|(NBCI ENTREZ)). InterPro: Cytochrome c class I (IPR003088). Pfam: cytochrome c. probable signal peptide. no TMHS; Conserved hypothetical protein; cytochrome c-552 2474945 4607315 azo2256 Azoarcus sp. BH72 cytochrome c-552 YP_933760.1 2474463 R 62928 CDS YP_933761.1 119898548 4607316 complement(2474953..2475486) 1 NC_008702.1 Probable methylamine dehydrogenase. Homology to mauD of P. denifrificans of 45% (CAA67190) carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin. InterPro: Methylamine dehydrogenase L subunit (IPR004229) Pfam: Methylamine dehydrogenase, L chain signal peptide no TMHs; High confidence in function and specificity; methylamine dehydrogenase, L chain 2475486 mauA 4607316 mauA Azoarcus sp. BH72 methylamine dehydrogenase, L chain YP_933761.1 2474953 R 62928 CDS YP_933762.1 119898549 4609196 complement(2475508..2476110) 1 NC_008702.1 Probable methylamine utilization protein. My be specifically involved in the processing transport and/or maturation of the methylamine dehydrogenase beta-subunit. Tigrfam: dsbE: periplasmic protein thiol:disulfide Signal peptide probable 1 TMHs; Family membership; methylamine utilization protein mauD 2476110 mauD 4609196 mauD Azoarcus sp. BH72 methylamine utilization protein mauD YP_933762.1 2475508 R 62928 CDS YP_933763.1 119898550 4609198 complement(2476107..2476658) 1 NC_008702.1 Methylamine utilization protein mauE. Homology to mauE of M. extorquens of 37% (sprot|MAUE_METEX) MAY BE SPECIFICALLY INVOLVED IN THE PROCESSING TRANSPORT AND/OR MATURATION OF THE MADH BETA-SUBUNIT. no signal peptide probable 4 TMHs; Family membership; putative methylamine utilization protein MauE 2476658 mauE 4609198 mauE Azoarcus sp. BH72 putative methylamine utilization protein MauE YP_933763.1 2476107 R 62928 CDS YP_933764.1 119898551 4609199 complement(2476658..2477812) 1 NC_008702.1 Methylamine dehydrogenase heavy chain precursor (MADH):- Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin. Entry name SWISSPROT:DHMH_METEX Prim. accession # Q49124 Identities = 100/339 (29%) Prediction: Signal peptide Signal peptide probability: 1.000 Number of predicted TMHs: 0; Family membership; putative methylamine dehydrogenase heavy chain 2477812 maub 4609199 maub Azoarcus sp. BH72 putative methylamine dehydrogenase heavy chain YP_933764.1 2476658 R 62928 CDS YP_933765.1 119898552 4609197 2478283..2479272 1 NC_008702.1 Putative ethanolamine operon transcriptional regulator,; Family membership; putative ethanolamine operon transcriptional regulator 2479272 eutR 4609197 eutR Azoarcus sp. BH72 putative ethanolamine operon transcriptional regulator YP_933765.1 2478283 D 62928 CDS YP_933766.1 119898553 4608688 2479608..2480288 1 NC_008702.1 Putative pyruvate dehydrogenase complex repressor,; Family membership; putative pyruvate dehydrogenase complex repressor 2480288 pdhR 4608688 pdhR Azoarcus sp. BH72 putative pyruvate dehydrogenase complex repressor YP_933766.1 2479608 D 62928 CDS YP_933767.1 119898554 4609515 2480340..2481158 1 NC_008702.1 Putative amino acid-binding protein. Homology to fliy of E. coli of 26% (AAC43545). PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR amino acids. InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR1638); solute binding protein/glutamte receptor (IPR001311) Pfam: Bacterial extracellular solute-binding protein signal peptide no TMHS hisG: ATP phosphoribosyltransferase; Specificity unclear; putative amino acid-binding protein 2481158 4609515 azo2263 Azoarcus sp. BH72 putative amino acid-binding protein YP_933767.1 2480340 D 62928 CDS YP_933768.1 119898555 4607317 2481229..2481897 1 NC_008702.1 Amino acid ABC transporter permease, 75% Identity to TrEMBL;Q87UQ0, 65% Identity to TrEMBL;Q8XXK9,Q6F6T8. Has PF00528; IPR000515, BPD_transp; Bacterial binding protein-dependent transport systems are multicomponent systems typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system. The integral inner-membrane proteins translocate the substrate across the membrane. It has been shown that most of these proteins contain a conserved region located about 80 to 100 residues from their C-terminal extremity. This region seems to be located in a cytoplasmic loop between two transmembrane domains. Apart from the conserved region,the sequence of these proteins is quite divergent, and they have a variable number of transmembrane helices,however they can be classified into seven families which have been respectively termed: araH, cysTW, fecCD, hisMQ,livHM, malFG and oppBC. Has PROSITE;PS50928; ABC_TM1; amino acid ABC transporter permease 2481897 4607317 azo2264 Azoarcus sp. BH72 amino acid ABC transporter permease YP_933768.1 2481229 D 62928 CDS YP_933769.1 119898556 4607318 2481897..2482550 1 NC_008702.1 Conserved hypothetical amino acid permease. Homology to pspto5247 of P. syringae (trembl|Q87UP9). PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM. PROBABLY RESPONSIBLE FOR THE TRANSLOCATION OF THE SUBSTRATE ACROSS THE MEMBRANE (BY SIMILARITY). Interpro: binding-protein-depenent transport system inner membrane component (IPR000515). Pfam: Binding-protein-dependent transport system. no signal peptide. 3 TMHs; Specificity unclear; amino acid permease 2482550 4607318 azo2265 Azoarcus sp. BH72 amino acid permease YP_933769.1 2481897 D 62928 CDS YP_933770.1 119898557 4607319 2482537..2483265 1 NC_008702.1 Probable glutamine transport ATP-binding protein. Homology with glnQ of B. stearothermophilus of 53% (sprot|GLNQ_BACST). PART OF THE BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR GLUTAMINE. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. InterPro: AAA ATPase superfamily (IPR003593), ABC_Transporter (IPR003439), ATP/GTP_binding site motif A (P-loop) (IPR001687) Pfam: ABC transporter no signal peptide no TMHs mobB: molybdopterin-guanine dinucleotid; High confidence in function and specificity; glutamine transport ATP-binding protein 2483265 glnQ2 4607319 glnQ2 Azoarcus sp. BH72 glutamine transport ATP-binding protein YP_933770.1 2482537 D 62928 CDS YP_933771.1 119898558 4608903 complement(2483290..2483919) 1 NC_008702.1 Probable Glutathione transferase. Homology to gstB of P. mirablilis of 29% (sprot|GT_PROMI). Catalysis of the reaction: R-X + glutathione = H-X + R-S-glutathione. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. InterPro: Glutathione S-transferase N terminus(IPR004045), Glutathione S-transferase C terminus (IPR004046) Pfam: Glutathione S-transferase, N-terminale domaine no signal peptide no TMHs; Family membership; putative glutathione transferase 2483919 gstB 4608903 gstB Azoarcus sp. BH72 putative glutathione transferase YP_933771.1 2483290 R 62928 CDS YP_933772.1 119898559 4608958 2484151..2484591 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to pp4833 of P. putida of 55% (trembl|Q88DJ2). no domains predicted. no signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 2484591 4608958 azo2268 Azoarcus sp. BH72 hypothetical protein YP_933772.1 2484151 D 62928 CDS YP_933773.1 119898560 4607320 2484632..2485495 1 NC_008702.1 Conserved hypothetical band 7 family protein. Homology to pp4834 of P.putida of 67% (trembl|Q88DJ1). InterPro: Band 7 protein (IPR001107). Pfam: SPFH domain / Band 7 family. The band 7 protein is an integral membrane protein which is thought to regulate cation conductance. A variety of proteins belong to this family. These include the prohibitins, cytoplasmic anti-proliferative proteins and stomatin, an erythrocyte membrane protein. Bacterial HflC protein also belongs to this family. probable 1 TMH. probable signal peptide (overlapping with the TMH); Family membership; hypothetical protein 2485495 4607320 azo2269 Azoarcus sp. BH72 hypothetical protein YP_933773.1 2484632 D 62928 CDS YP_933774.1 119898561 4607321 complement(2485527..2487548) 1 NC_008702.1 Methyl-accepting chemotaxis transducer,; Specificity unclear; methyl-accepting chemotaxis transducer 2487548 4607321 azo2270 Azoarcus sp. BH72 methyl-accepting chemotaxis transducer YP_933774.1 2485527 R 62928 CDS YP_933775.1 119898562 4607322 2487857..2488717 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003250 of Dechloromonas aromatica of 32% (gi|41723264|ref|ZP_00150207.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 2488717 4607322 azo2271 Azoarcus sp. BH72 hypothetical protein YP_933775.1 2487857 D 62928 CDS YP_933776.1 119898563 4607323 complement(2488755..2490671) 1 NC_008702.1 Low affinity potassium transport system protein,Kup. In E.coli, Kup has two domains, the first aa residues form an integral membrane domain and the C-terminal residues form a hydrophilic ones located at the citoplasmic side of the membrane. Kup: involved in potassium uptake and metabolism.; High confidence in function and specificity; putative potassium uptake protein 2490671 kup 4607323 kup Azoarcus sp. BH72 putative potassium uptake protein YP_933776.1 2488755 R 62928 CDS YP_933777.1 119898564 4609108 complement(2490788..2491693) 1 NC_008702.1 Conserved hypothetical protein:- Entry name:-TREMBL:Q89MA9 Prim. accession # Q89MA9 InterPro:-IPR000379; Ser_estrs. Identities = 120/294 (40%) Pfam:-Thioesterase domain Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; hypothetical protein 2491693 4609108 azo2273 Azoarcus sp. BH72 hypothetical protein YP_933777.1 2490788 R 62928 CDS YP_933778.1 119898565 4607324 complement(2491893..2492903) 1 NC_008702.1 Sulfate-binding protein precursor, Sbp. E.coli Sulfate starvation-induced protein 2,SSI2. This protein specifically binds sulfate and is involved in its transmembrane transport. InterPro: Prokaryotic sulfate- /thiosulfate-binding protein. In other bacteria Sbp is a periplasmic component of an ABC transport system. TIGR00363: lipoprotein YaeC family Signal peptide; High confidence in function and specificity; putative sulfate-binding protein 2492903 sbp1 4607324 sbp1 Azoarcus sp. BH72 putative sulfate-binding protein YP_933778.1 2491893 R 62928 CDS YP_933779.1 119898566 4609841 complement(2493040..2493828) 1 NC_008702.1 Hypothetical protein ycjL. TREMBL:Q890B8: 38% identity, 53% similarity. Glutamine amidotransferase activity involves the removal of the ammonia group from a glutamate molecule and its subsequent transfer to a pecific substrate, thus creating a new carbon-nitrogen group on the substrate. InterPro:IPR000991; GATase_1 PROSITE PS00442 GATASE_TYPE_I BLOCKS: BL00442 PFAM:PF00117 GATase; PF00310 GATase_2 trpG_papA: glutamine amidotransferase o No signal peptide (SignalP predicted) No transmembrane helices; High confidence in function and specificity; putative glutamine amidotransferase 2493828 4609841 azo2275 Azoarcus sp. BH72 putative glutamine amidotransferase YP_933779.1 2493040 R 62928 CDS YP_933780.1 119898567 4607325 complement(2493825..2494952) 1 NC_008702.1 ATP-dependent; carboxylate-amine ligase with weak glutamate--cysteine ligase activity; carboxylate-amine ligase 2494952 ybdK 4607325 ybdK Azoarcus sp. BH72 carboxylate-amine ligase YP_933780.1 2493825 R 62928 CDS YP_933781.1 119898568 4610145 complement(2494949..2496157) 1 NC_008702.1 Conserved hypothetical Na/H antiporter. Homology to Bucepa03004091 of B.cepacia of 41% (gi|46320868|ref|ZP_00221251.1|(NBCI ENTREZ)). Has PF00999 Sodium/hydrogen exchanger family; IPR006153. Na/H antiporters are key transporters in maintaining the pH of actively metabolising cells. The molecular mechanisms of antiport are unclear. These antiporters contain 10-12 transmembrane regions (M) at the amino-terminus and a large cytoplasmic region at the carboxyl terminus. The transmembrane regions M3-M12 share identity with other members of the family. The M6 and M7 regions are highly conserved. Thus, this is thought to be the region that is involved in the transport of sodium and hydrogen ions. The cytoplasmic region has little similarity throughout the family. No signal peptide. 12 TMHs.; Conserved hypothetical protein; Na/H antiporter 2496157 4610145 azo2277 Azoarcus sp. BH72 Na/H antiporter YP_933781.1 2494949 R 62928 CDS YP_933782.1 119898569 4607326 2496728..2498482 1 NC_008702.1 Conserved hypothetical protein. Homology to pa3943 of P. aeruginosa (TREMBL:Q9HX73). no domians predicted. weak evidence for signal peptide. no TMHs; hypothetical protein 2498482 4607326 azo2278 Azoarcus sp. BH72 hypothetical protein YP_933782.1 2496728 D 62928 CDS YP_933783.1 119898570 4607327 2498525..2499364 1 NC_008702.1 Conserved hypothetical protein. Homology to Avin02000067 of Azotobacter vinelandii of 50% (gi|23105742|ref|ZP_00092196.1|(NBCI ENTREZ)). InterPro: Ankyrin-repeat. The ankyrin repeat is one of the most common protein-protein interaction motifs in nature. Ankyrin repeats are tandemly repeated modules of about 33 amino acids.The ankyrin fold appears to be defined by its structure rather than its function since there is no specific sequence or structure which is universally recognised by it. The conserved fold of the ankyrin repeat unit is known from several crystal and solution structures. Each repeat folds into a helix-loop-helix structure with a beta-hairpin/loop region projecting out from the helices at a 90 angle. The repeats stack together to form an L-shaped structure. No signal peptide. No TMHs.; hypothetical protein 2499364 4607327 azo2279 Azoarcus sp. BH72 hypothetical protein YP_933783.1 2498525 D 62928 CDS YP_933784.1 119898571 4607328 2499372..2499698 1 NC_008702.1 Hypothetical protein 25% Identity to TrEMBL;Q7U749. No domains, repeats, motifs or features present; hypothetical protein 2499698 4607328 azo2280 Azoarcus sp. BH72 hypothetical protein YP_933784.1 2499372 D 62928 CDS YP_933785.1 119898572 4607329 2499793..2500329 1 NC_008702.1 An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group; flavodoxin FldA 2500329 nifF2 4607329 nifF2 Azoarcus sp. BH72 flavodoxin FldA YP_933785.1 2499793 D 62928 CDS YP_933786.1 119898573 4609362 2500478..2503111 1 NC_008702.1 Conserved hypothetical protein. Identity to Daro_1071 of Dechloromonas aromatica of 41% (gi|71846331|gb|AAZ45827.1|). Pfam: CheW-like domain. CheW-like domain proteins are part of the chemotaxis signaling mechanism in bacteria. CheW interacts with the methyl accepting chemotaxis proteins (MCPs) and relays signals to CheY, which affects flageller rotation. This family includes CheW and other related proteins that are involved in chemotaxis. The CheW-like regulatory domain in CheA binds to CheW, suggesting that these domains can interact with each other.; hypothetical protein 2503111 4609362 azo2282 Azoarcus sp. BH72 hypothetical protein YP_933786.1 2500478 D 62928 CDS YP_933787.1 119898574 4607330 2503186..2503836 1 NC_008702.1 Similar to TREMBL:Q92PT6 (40% identical); TREMBL:Q98MM5 (39% identical).; hypothetical protein 2503836 4607330 azo2283 Azoarcus sp. BH72 hypothetical protein YP_933787.1 2503186 D 62928 CDS YP_933788.1 119898575 4607331 complement(2503871..2504842) 1 NC_008702.1 Similar to TREMBL:Q9RBR1 (43% identity); TREMBL:Q8GN85 (42% identity); TREMBL:Q87NF7 (23% identity). SignalP predicting signal peptide.; Specificity unclear; putative periplasmic substrate binding protein 2504842 4607331 azo2284 Azoarcus sp. BH72 putative periplasmic substrate binding protein YP_933788.1 2503871 R 62928 CDS YP_933789.1 119898576 4607332 complement(2504880..2506196) 1 NC_008702.1 The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 38% DctM.IPR000252; DedA.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1. Signal peptide:present. TMhelix:11.; Specificity unclear; putative TRAP-type C4-dicarboxylate transport system large permease 2506196 dctM3 4607332 dctM3 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system large permease YP_933789.1 2504880 R 62928 CDS YP_933790.1 119898577 4608599 complement(2506198..2506716) 1 NC_008702.1 TRAP-type C4-dicarboxylate transport system, small permease component. The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate.40% DctQ. Pfam:PF04290; DctQ; 1. TMHelix:3.; Function unclear; putative TRAP-type C4-dicarboxylate transport system small permease 2506716 dctQ3 4608599 dctQ3 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system small permease YP_933790.1 2506198 R 62928 CDS YP_933791.1 119898578 4608610 complement(2506856..2507242) 1 NC_008702.1 Conserved hypothetical protein. Homology to tll1086 of T.elongatus of 62% (trembl:Q8DJY4). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2507242 4608610 azo2287 Azoarcus sp. BH72 hypothetical protein YP_933791.1 2506856 R 62928 CDS YP_933792.1 119898579 4607333 complement(2507315..2508640) 1 NC_008702.1 HD-domain containing protein,; Conserved hypothetical protein; HD-domain-containing protein 2508640 4607333 azo2288 Azoarcus sp. BH72 HD-domain-containing protein YP_933792.1 2507315 R 62928 CDS YP_933793.1 119898580 4610018 2509010..2509729 1 NC_008702.1 Mg(2+) transport ATPase [mgtC family protein], 35% identity(54% similarity) to TrEMBL;Q7NTN3. Has PF02308,MgtC family;(IPR003416, MgtCSapB_transpt);The MgtC protein is found in an operon with the Mg2+ transporter protein MgtB. The function of MgtC and its homologues is not known, but it is thought that MgtC may act as an accessory protein for MgtB, thus mediating magnesium influx into the cytosol. Also included in this family are the Bacillus subtilis SapB protein and several hypothetical proteins. NO Signal Peptide or TMH present.; Family membership; putative transport ATPase 2509729 4610018 azo2289 Azoarcus sp. BH72 putative transport ATPase YP_933793.1 2509010 D 62928 CDS YP_933794.1 119898581 4607335 2509855..2510652 1 NC_008702.1 Conserved hypothetical protein. Homology to hypothetical protein all0327 of Anabaena sp. of 52%. no signal peptid. no TMHs. No domains predicted.; hypothetical protein 2510652 4607335 azo2290 Azoarcus sp. BH72 hypothetical protein YP_933794.1 2509855 D 62928 CDS YP_933795.1 119898582 4607336 2510662..2511024 1 NC_008702.1 Conserved hypothetical DNA transformation protein,; Conserved hypothetical protein; DNA transformation protein 2511024 tfoX 4607336 tfoX Azoarcus sp. BH72 DNA transformation protein YP_933795.1 2510662 D 62928 CDS YP_933796.1 119898583 4609931 2511136..2512284 1 NC_008702.1 Methylated-DNA--[protein]-cysteine S-methyltransferase (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase) InterPro: Methylated-DNA--protein-cysteine methyltransferase; High confidence in function and specificity; methylated-DNA--[protein]-cysteine S-methyltransferase 2512284 ada 4609931 ada Azoarcus sp. BH72 methylated-DNA--[protein]-cysteine S-methyltransferase YP_933796.1 2511136 D 62928 CDS YP_933797.1 119898584 4608277 2512299..2512964 1 NC_008702.1 Alkylated DNA repair protein.Probably involved in the repair of alkylated DNA. Provides extensive resistance to alkylating agents.; High confidence in function and specificity; DNA-directed DNA polymerase 2512964 alkB 4608277 alkB Azoarcus sp. BH72 DNA-directed DNA polymerase YP_933797.1 2512299 D 62928 CDS YP_933798.1 119898585 4608303 2513048..2514142 1 NC_008702.1 Putative Zinc carboxypeptidase-related protein, 45% identity to TrEMBL;Q6MKH4,Zinc carboxypeptidase-related protein (EC 3.4.17.-) [Bd2418] [Bdellovibrio bacteriovorus] Has pfam00246, Zn_carbOpept, Zinc carboxypeptidase domain.; High confidence in function and specificity; carboxypeptidase A 2514142 4608303 azo2294 Azoarcus sp. BH72 carboxypeptidase A YP_933798.1 2513048 D 62928 CDS YP_933799.1 119898586 4607337 2514180..2514956 1 NC_008702.1 TREMBL:Q8EFJ6: 33% identity, 47% similarity. Probable Dihydrolipoamide acetyltransferase component of acetoin cleaving system (EC 2.3.1.12) (Acetoin dehydrogenase E2 component). Interpro:IPR000073; A/b_hydrolase (alpha/beta fold family). IPR000379; Ser_estrs. Pfam PF00561; Abhydrolase_1; 1 2a75: L-lysine exporter; Function unclear; putative hydrolase 2514956 4607337 azo2295 Azoarcus sp. BH72 putative hydrolase YP_933799.1 2514180 D 62928 CDS YP_933800.1 119898587 4607338 2515151..2515504 1 NC_008702.1 PHS (4-alpha-hydroxy-tetrahydropterin dehydratase) (Pterin carbinolamine dehydratase) (PCD). InterPro: Pterin 4 alpha carbinolamine dehydratase; High confidence in function and specificity; 4a-hydroxytetrahydrobiopterin dehydratase 2515504 phhB 4607338 phhB Azoarcus sp. BH72 4a-hydroxytetrahydrobiopterin dehydratase YP_933800.1 2515151 D 62928 CDS YP_933801.1 119898588 4609548 2515532..2515825 1 NC_008702.1 Conserved hypothetical protein. Homology to RS05664 of R.solanacearum of 53% (trembl:Q8XQC7). No domains predicted. No signal peptide or TMH reported present.; hypothetical protein 2515825 4609548 azo2297 Azoarcus sp. BH72 hypothetical protein YP_933801.1 2515532 D 62928 CDS YP_933802.1 119898589 4607339 2515850..2516959 1 NC_008702.1 Conserved hypothetical protein. Homology to PA1356 of Pseudomonas aeruginosa of 69% (trembl|Q9I3Y8(SRS)). No domains predicted. No signal peptide or TMH reported to be present.; hypothetical protein 2516959 4607339 azo2298 Azoarcus sp. BH72 hypothetical protein YP_933802.1 2515850 D 62928 CDS YP_933803.1 119898590 4607340 2516981..2518624 1 NC_008702.1 Aidb protein:-belongs to the acyl-coa dehydrogenase family. Catalysis of the reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Entry name SWISSPROT:AIDB_ECOLI Prim. accession # P33224 Identities = 257/515 (49%) InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; 1 Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; acyl-CoA dehydrogenase 2518624 aidB1 4607340 aidB1 Azoarcus sp. BH72 acyl-CoA dehydrogenase YP_933803.1 2516981 D 62928 CDS YP_933804.1 119898591 4608289 2519006..2520262 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ORF428 of Roseateles depolymerans of 65% (trembl|Q9F206(SRS)). Pfam: Protein of unknown function (DUF1504). This family consists of several hypothetical bacterial proteins of around 440 residues in length. The function of this family is unknown. signal peptide. 10 TMHs; Conserved hypothetical protein; hypothetical protein 2520262 4608289 azo2300 Azoarcus sp. BH72 hypothetical protein YP_933804.1 2519006 D 62928 CDS YP_933805.1 119898592 4607341 2520561..2522381 1 NC_008702.1 General secretion pathway protein E,; Specificity unclear; general secretory pathway protein E 2522381 gspE3 4607341 gspE3 Azoarcus sp. BH72 general secretory pathway protein E YP_933805.1 2520561 D 62928 CDS YP_933806.1 119898593 4608951 2522542..2523762 1 NC_008702.1 Conserved hypothetical protein. Homology to Mll6421 of M.loti of 37% (trembl:Q989H3). Has PF02661, Fic protein family;IPR003812:This family consists of the Fic (filamentation induced by cAMP) protein and its relatives. The Fic protein is involved in cell division and is suggested to be involved in the synthesis of PAB or folate, indicating that the Fic protein and cAMP are involved in a regulatory mechanism of cell division via folate metabolism. This family contains a central conserved motif HPFXXGNG in most members. The exact molecular function of these proteins is uncertain. No signal peptide or TMH present.; hypothetical protein 2523762 4608951 azo2302 Azoarcus sp. BH72 hypothetical protein YP_933806.1 2522542 D 62928 CDS YP_933807.1 119898594 4607342 complement(2523759..2525189) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Daro03002720 of Dechloromonas aromatica of 55% (gi|41723618|ref|ZP_00150528.1|(NBCI ENTREZ)). Permease,member of the Major Facilitator Superfamiliy (MFS)transporters.MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. Similar to proteins involved in tetracycline resistance. Hypothetical transport protein yhcA, from B.subtilis. InterPro: Drug resistance transporter EmrB/QacA subfamily efflux_EmrB: drug resistance transport. Signal peptide predicted. 13 TMHs; Function unclear; hypothetical protein 2525189 4607342 azo2303 Azoarcus sp. BH72 hypothetical protein YP_933807.1 2523759 R 62928 CDS YP_933808.1 119898595 4607343 complement(2525577..2526161) 1 NC_008702.1 Transcriptional regulator, MerR-family InterPro: Bacterial regulatory protein, MerR, Putative DNA binding HTH reporting nucleic acid binding motif; MerR family transcriptional regulator 2526161 4607343 azo2304 Azoarcus sp. BH72 MerR family transcriptional regulator YP_933808.1 2525577 R 62928 CDS YP_933809.1 119898596 4607344 complement(2526158..2526574) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV1954 of Chromobacterium violaceum of 33% (trembl:Q7NWM9). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2526574 4607344 azo2305 Azoarcus sp. BH72 hypothetical protein YP_933809.1 2526158 R 62928 CDS YP_933810.1 119898597 4607345 2526840..2527454 1 NC_008702.1 Tyrosine recombinase xerD. Site-specific tyrosine recombinase which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from xerC binding sites by a short central region forming the heterotetrameric xerC-xerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. In the complex xerD specifically exchanges the bottom DNA strands (By similarity). InterPro: Phage integrase; Specificity unclear; putative tyrosine recombinase xerD 2527454 4607345 azo2306 Azoarcus sp. BH72 putative tyrosine recombinase xerD YP_933810.1 2526840 D 62928 CDS YP_933811.1 119898598 4607346 2527848..2531426 1 NC_008702.1 Hypothetical protein, 39% similarity to TrEMBL;Q8Z0Z1. No Signal Peptide or TMH present. TIGR00090: iojap-related protein; hypothetical protein 2531426 4607346 azo2307 Azoarcus sp. BH72 hypothetical protein YP_933811.1 2527848 D 62928 CDS YP_933812.1 119898599 4607347 2531423..2537752 1 NC_008702.1 Hypothetical protein yjiV. TREMBL:Q8XC75: 33% identity, 49% similarity InterPro: IPR001410;DEAD/DEAH box helicase IPR001650; Helicase_C. Pfam: PF00270; DEAD; 1. PF00271; Helicase_C; 1. SMART: SM00487; DEXDc; 1. SM00490; HELICc L18_bact: ribosomal protein L18 No signal peptide No transmembrane helices; High confidence in function and specificity; putative helicase related protein 2537752 4607347 azo2308 Azoarcus sp. BH72 putative helicase related protein YP_933812.1 2531423 D 62928 CDS YP_933813.1 119898600 4607348 2537814..2538605 1 NC_008702.1 Conserved hypothetical protein. 61% identity to hypothetical protein Bucepa02000624 [Burkholderia cepacia R1808] (gi|46324486|ref|ZP_00224847.1|(NBCI ENTREZ)). NO Signal peptide or TMH reported present. no domains predicted; hypothetical protein 2538605 4607348 azo2309 Azoarcus sp. BH72 hypothetical protein YP_933813.1 2537814 D 62928 CDS YP_933814.1 119898601 4607349 complement(2538956..2540617) 1 NC_008702.1 Alpha -D-1,4-glucosidase, PalZ. Involved in the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. 55% Alpha_amyl_cat.IPR006589; Alp_amyl_cat_sub.IPR006046; Glyco_hydro_13. Pfam:PF00128; Alpha-amylase;1.; High confidence in function and specificity; putative alpha-D-1,4-glucosidase 2540617 palZ 4607349 palZ Azoarcus sp. BH72 putative alpha-D-1,4-glucosidase YP_933814.1 2538956 R 62928 CDS YP_933815.1 119898602 4609492 2540718..2541824 1 NC_008702.1 Glucan 13-beta-glucosidase precursor (EC 3.2.1.58) (Exo-13-beta- glucanase).BETA-GLUCANASES PARTICIPATE IN THE METABOLISM OF BETA-GLUCAN THE MAIN STRUCTURAL COMPONENT OF THE CELL WALL. IT COULD ALSO FUNCTION BIOSYNTHETICALLY AS A TRANSGLYCOSYLASE. 33% Glyco_hydro_5. Pfam:PF00150; Cellulase; 1.; High confidence in function and specificity; putative glucan 1,3-beta-glucosidase 2541824 spr1 4609492 spr1 Azoarcus sp. BH72 putative glucan 1,3-beta-glucosidase YP_933815.1 2540718 D 62928 CDS YP_933816.1 119898603 4609890 complement(2541810..2543588) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Avin02002177 of Azotobacter vinelandii of 61% (gi|53611929|ref|ZP_00090985.2|(NBCI ENTREZ)). InterPro: ABC transporter (IPR003439). Pfam: ABC transporter. no signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 2543588 4609890 azo2312 Azoarcus sp. BH72 hypothetical protein YP_933816.1 2541810 R 62928 CDS YP_933817.1 119898604 4607350 complement(2543591..2544544) 1 NC_008702.1 Glucokinase (EC 2.7.1.2) (Glucose kinase). Catalytic activity: ATP + d-glucose = ADP+ d-glucose 6-phosphate. 24% Glucokinase. Pfam:PF02685; Glucokinase; 1. TIGRFAMs:TIGR00749; glk; 1.; High confidence in function and specificity; putative glucokinase 2544544 glk2 4607350 glk2 Azoarcus sp. BH72 putative glucokinase YP_933817.1 2543591 R 62928 CDS YP_933818.1 119898605 4608890 2544700..2546202 1 NC_008702.1 Family membership; glycosyltransferase 2546202 4608890 azo2314 Azoarcus sp. BH72 glycosyltransferase YP_933818.1 2544700 D 62928 CDS YP_933819.1 119898606 4607351 2546172..2546876 1 NC_008702.1 Conserved hypothetical tyrosine/serine phosphatase,; Conserved hypothetical protein; putative tyrosine/serine phosphatase 2546876 4607351 azo2315 Azoarcus sp. BH72 putative tyrosine/serine phosphatase YP_933819.1 2546172 D 62928 CDS YP_933820.1 119898607 4607352 2546873..2548639 1 NC_008702.1 Lipid A export ATP-binding/permease msbA. MsbA (TC 3.A.1.106.1) is involved in lipid A and possibly also glycerophospholipids export. The transmembrane domain (TMD) forms a pore in the inner membrane and the ATP-binding domain (NBD) is responible for energy generation. Similar to TREMBL:Q8ZGA9; TREMBL:Q8XXB6; SWISSPROT:P60752 (37% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003593): AAA ATPase. InterPro (IPR001140): ABC transporter transmembrane region. Pfam (PF00664): ABC transporter transmembrane region. Pfam (PF00005): ABC transporter. TMHMM reporting five transmembrane helices. TC (3.A.1.106): The Lipid Exporter (LipidE) Family.; High confidence in function and specificity; Lipid A export ATP-binding/permease 2548639 msbA1 4607352 msbA1 Azoarcus sp. BH72 Lipid A export ATP-binding/permease YP_933820.1 2546873 D 62928 CDS YP_933821.1 119898608 4609275 2548636..2549829 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology of the entire protein to the data bank. no dmains predicted. no signal peptide. no TMHs; hypothetical protein 2549829 4609275 azo2317 Azoarcus sp. BH72 hypothetical protein YP_933821.1 2548636 D 62928 CDS YP_933822.1 119898609 4607353 2549826..2551112 1 NC_008702.1 Hypothetical membrane protein. homology to rsc2204 of R.solanacearum of 28% (trembl|Q8XXB2(SRS)). no domains predicted. no signal peptide. 10 TMHs; hypothetical protein 2551112 4607353 azo2318 Azoarcus sp. BH72 hypothetical protein YP_933822.1 2549826 D 62928 CDS YP_933823.1 119898610 4607354 2551109..2551732 1 NC_008702.1 CMP-N-acetylneuraminate-beta-galactosamide-alpha- 23- sialyltransferase (EC 2.4.99.4) (Beta-galactoside alpha-23-sialyltransferase) (Alpha 23-ST) (Gal-NAc6S) (Gal-beta-13-GalNAc-alpha-23-sialyltransferase) (ST3GalIA) (ST3O) (ST3GalA.1) (SIAT4-A) (ST3Gal I). IT MAY BE RESPONSIBLE FOR THE SYNTHESIS OF THE SEQUENCE NEUAC-ALPHA-23-GAL-BETA-13-GALNAC- FOUND ON SUGAR CHAINS O-LINKED TO THR OR SER AND ALSO AS A TERMINAL SEQUENCE ON CERTAIN GANGLIOSIDES. SIAT4-A AND SIAT4-B SIALYLATE THE SAME ACCEPTOR SUBSTRATES BUT EXHIBIT DIFFERENT KM VALUES.; Specificity unclear; glycosyltransferase 2551732 4607354 azo2319 Azoarcus sp. BH72 glycosyltransferase YP_933823.1 2551109 D 62928 CDS YP_933824.1 119898611 4607355 2551737..2552735 1 NC_008702.1 Conserved hypothetical protein. Homology to Rsph03001342 of Rhodobacter sphaeroides of 39% (gi|22957735|ref|ZP_00005426.1|(NBCI ENTREZ)). Has PF04230, Polysaccharide pyruvyl transferase;IPR007345 PS_pyruv_trans; Pyruvyl-transferases involved in peptidoglycan-associated polymer biosynthesis. CsaB in Bacillus anthracis is necessary for the non-covalent anchoring of proteins containing an SLH (S-layer homology) domain to peptidoglycan-associated pyruvylated polysaccharides. WcaK and AmsJ are involved in the biosynthesis of colanic acid in Escherichia coli and of amylovoran in Erwinia amylovora. No signal peptide. No TMHs; hypothetical protein 2552735 4607355 azo2320 Azoarcus sp. BH72 hypothetical protein YP_933824.1 2551737 D 62928 CDS YP_933825.1 119898612 4607356 2552955..2554031 1 NC_008702.1 High confidence in function and specificity; lipopolysaccharide heptosyltransferase I 2554031 waaC1 4607356 waaC1 Azoarcus sp. BH72 lipopolysaccharide heptosyltransferase I YP_933825.1 2552955 D 62928 CDS YP_933826.1 119898613 4610101 2554028..2555143 1 NC_008702.1 Hypothetical glycosyl transferase MJ1607 (EC 2.-.-.-). InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 2555143 4610101 azo2322 Azoarcus sp. BH72 glycosyltransferase YP_933826.1 2554028 D 62928 CDS YP_933827.1 119898614 4607357 2555172..2556242 1 NC_008702.1 Lipopolysaccharide core biosynthesis glycosyl transferase lpsE (EC 2.-.-.-).; Specificity unclear; glycosyltransferase 2556242 4607357 azo2323 Azoarcus sp. BH72 glycosyltransferase YP_933827.1 2555172 D 62928 CDS YP_933828.1 119898615 4607358 2556244..2557083 1 NC_008702.1 29% Polysac_deacet. Pfam:PF01522; Polysacc_deac_1; 1.; High confidence in function and specificity; putative polysaccharide deacetylase 2557083 4607358 azo2324 Azoarcus sp. BH72 putative polysaccharide deacetylase YP_933828.1 2556244 D 62928 CDS YP_933829.1 119898616 4607359 2557095..2557580 1 NC_008702.1 Conserved hypothetical protein. Homology to CV1439 of Chromobacterium violaceum of 58% (trembl:Q7NY36). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2557580 4607359 azo2325 Azoarcus sp. BH72 hypothetical protein YP_933829.1 2557095 D 62928 CDS YP_933830.1 119898617 4607360 2557664..2559307 1 NC_008702.1 Conserved hypothetical signaling protein,; Conserved hypothetical protein; putative signaling protein 2559307 4607360 azo2326 Azoarcus sp. BH72 putative signaling protein YP_933830.1 2557664 D 62928 CDS YP_933831.1 119898618 4607361 complement(2559320..2560276) 1 NC_008702.1 HTH-type transcriptional regulator cbl (Cys regulon transcriptional activator) Belongs to the LysR family of transcriptional regulators. THIS PROTEIN IS A POSITIVE REGULATOR OF GENE EXPRESSION FOR THE CYSTEINE REGULON. THE INDUCER FOR CYSB IS N-ACETYLSERINE (BY SIMILARITY). ribD_Cterm: riboflavin-specific deami 42% similarity to the HTH-type transcriptional regulator cbl, E. coli SWISSPROT:CBL_ECOLI IPR000847; HTH_LysR. IPR005119; LysR_subst. PF00126; HTH_1; 1. helix-turn-helix PF03466; LysR_substrate; 1. HTH reporting nucleic acid binding motif; High confidence in function and specificity; transcriptional regulator CysB-like protein 2560276 cbl 4607361 cbl Azoarcus sp. BH72 transcriptional regulator CysB-like protein YP_933831.1 2559320 R 62928 CDS YP_933832.1 119898619 4608437 2560607..2561695 1 NC_008702.1 Probable transcriptional regulator,; Specificity unclear; transcriptional regulator 2561695 pspF 4608437 pspF Azoarcus sp. BH72 transcriptional regulator YP_933832.1 2560607 D 62928 CDS YP_933833.1 119898620 4609639 2561964..2562611 1 NC_008702.1 For the transport of bound iron across the inner membrane, a receptor complex is needed. The major component of this is tonB, a 27kDa protein that facilitates energy transfer from the proton motive force to outer membrane receptors. InterPro: Proline-rich region; Function unclear; periplasmic biopolymer transport protein linking inner and outer membranes 2562611 tonB2 4609639 tonB2 Azoarcus sp. BH72 periplasmic biopolymer transport protein linking inner and outer membranes YP_933833.1 2561964 D 62928 CDS YP_933834.1 119898621 4609963 2562648..2563469 1 NC_008702.1 Putative biopolymer transport protein ExbB. Homology to exbB of B. pertussis of 36%. ExbB is part of the TonB-dependent transduction complex. The tonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. Pfam: MotA/TolQ/ExbB proton channel family signal peptide most probable 4TMHs; Family membership; putative biopolymer transport protein ExbB 2563469 exbB4 4609963 exbB4 Azoarcus sp. BH72 putative biopolymer transport protein ExbB YP_933834.1 2562648 D 62928 CDS YP_933835.1 119898622 4608699 2563476..2563895 1 NC_008702.1 Putative biopolymer transport protein exbD. Homology to exbD of E. coli of 34%. ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; Family membership; putative biopolymer transport protein ExbD 2563895 exbD5 4608699 exbD5 Azoarcus sp. BH72 putative biopolymer transport protein ExbD YP_933835.1 2563476 D 62928 CDS YP_933836.1 119898623 4608704 2563914..2564342 1 NC_008702.1 Putative biopolymer transport protein. Homology to exbD1 of X. campestris of 31%. ExbD is part of the TonB-dependent transduction complex. The TonB complex uses the proton gradient across the inner bacterial membrane to transport large molecules across the outer bacterial membrane. InterPro: Biopolymer transport protein ExbD/TolR Pfam: Biopolymer transport protein ExbD/TolR no signal peptide probable 1 TMH; Family membership; putative biopolymer transport protein ExbD 2564342 exbD6 4608704 exbD6 Azoarcus sp. BH72 putative biopolymer transport protein ExbD YP_933836.1 2563914 D 62928 CDS YP_933837.1 119898624 4608705 2564464..2565738 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family, TREMBL:Q7WF48 (49% identity); TREMBL:Q881X8 (49% identity). Pfam (PF00529): HlyD family secretion protein. TMHMM reporting one transmembrane helix. TC (8.A.1): The Membrane Fusion Protein (MFP) Family.; Family membership; membrane fusion protein 2565738 4608705 azo2333 Azoarcus sp. BH72 membrane fusion protein YP_933837.1 2564464 D 62928 CDS YP_933838.1 119898625 4607362 2565735..2568839 1 NC_008702.1 AcrB/AcrD/AcrF family protein. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer,SWISSPROT:P76399 (44% identity). Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TMHMM reporting 10 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; High confidence in function and specificity; RND efflux transporter permease 2568839 4607362 azo2334 Azoarcus sp. BH72 RND efflux transporter permease YP_933838.1 2565735 D 62928 CDS YP_933839.1 119898626 4607363 2568839..2571973 1 NC_008702.1 AcrB/AcrD/AcrF family member. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q881X7 (44% identity); SWISSPROT:P76399 (48% identity). InterPro (IPR001036): Acriflavin resistance protein Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TMHMM reporting 10 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family. HTH reporting nucleic acid binding motif.; Specificity unclear; RND efflux transporter permease 2571973 4607363 azo2335 Azoarcus sp. BH72 RND efflux transporter permease YP_933839.1 2568839 D 62928 CDS YP_933840.1 119898627 4607364 2572063..2573526 1 NC_008702.1 Putative outer membrane efflux protein. Homology to opcM of B. cepacia of 36%. Component of an efflux system that confers multiple antibiotic resistence. Pfam: outer membrane efflux protein signal peptide no TMHs; Family membership; putative outer membrane efflux protein 2573526 opcM1 4607364 opcM1 Azoarcus sp. BH72 putative outer membrane efflux protein YP_933840.1 2572063 D 62928 CDS YP_933841.1 119898628 4609451 2573541..2574254 1 NC_008702.1 Conserved hypothetical hydrogenase cytochrone b-type subunit. Homology to C. vinosum of 48% (trembl|Q46471). Involved in electron transfer from hydrogen to oxygen. Pfam: Nickel-dependent hydrogenase b-type cytochrome. probable signal peptide. probable 4 TMHs; Family membership; hydrogenase cytochrome b-type subunit 2574254 4609451 azo2337 Azoarcus sp. BH72 hydrogenase cytochrome b-type subunit YP_933841.1 2573541 D 62928 CDS YP_933842.1 119898629 4607365 2574305..2576401 1 NC_008702.1 Tail-specific protease precursor (EC 3.4.21.102) (Protease Re) (PRC protein) (C-terminal processing peptidase). prc: carboxyl-terminal protease; High confidence in function and specificity; tail-specific penicillin-binding protein protease 2576401 prc 4607365 prc Azoarcus sp. BH72 tail-specific penicillin-binding protein protease YP_933842.1 2574305 D 62928 CDS YP_933843.1 119898630 4609622 complement(2577133..2577597) 1 NC_008702.1 Probable cytochrome c'. Homology to cycA of C. vinosum of 48% (sprot|CYCP_CHRVI). CYTOCHROME C IS THE MOST WIDELY OCCURRING BACTERIAL C-TYPE CYTOCHROME. CYTOCHROMES C ARE HIGH-SPIN PROTEINS AND THE HEME HAS NO SIXTH LIGAND. THEIR EXACT FUNCTION IS NOT KNOWN. InterPro: Cytochrome c class II (IPR002321) Pfam: Cytochrome c singal peptide no TMHs; Function unclear; cytochrome c' 2577597 cycA2 4609622 cycA2 Azoarcus sp. BH72 cytochrome c' YP_933843.1 2577133 R 62928 CDS YP_933844.1 119898631 4608549 complement(2577644..2578648) 1 NC_008702.1 Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway, TREMBL:Q8XBV0 (48% identity); TREMBL:Q8EI44 (45% identity). InterPro (IPR002925): Dienelactone hydrolase. InterPro (IPR000379): Esterase/lipase/thioesterase. Pfam (PF01738): Dienelactone hydrolase family. SignalP predicting signal peptide.; Family membership; dienelactone hydrolase family protein 2578648 4608549 azo2340 Azoarcus sp. BH72 dienelactone hydrolase family protein YP_933844.1 2577644 R 62928 CDS YP_933845.1 119898632 4607366 complement(2578692..2579669) 1 NC_008702.1 Gluconolactonase precursor (D-glucono-delta-lactone lactonohydrolase).HYDROLYZES THE GLUCONOLACTONE FORMED BY GLUCOSE-FRUCTOSE OXIDOREDUCTASE AND THAT FORMED IN AEROBIC CONDITIONS BY THE GLUCOSE DEHYDROGENASE PRESENT. 30% SMP-30. Pfam:PF03758; SMP-30; 1. Signal peptide: present.; High confidence in function and specificity; putative gluconolactonase 2579669 gnl 4607366 gnl Azoarcus sp. BH72 putative gluconolactonase YP_933845.1 2578692 R 62928 CDS YP_933846.1 119898633 4608929 complement(2579886..2580566) 1 NC_008702.1 Putative cytochrome c4. Homology to cc4 of A. vinelandii of 34% (sprot|CYC4_AZOVI). Diheme, high potential cytochrome c believed to be an intermediate electron donor to terminal oxidation systems. Pfam: cytochrome C signal peptide no TMHs; Family membership; putative cytochrome c4 2580566 cc41 4608929 cc41 Azoarcus sp. BH72 putative cytochrome c4 YP_933846.1 2579886 R 62928 CDS YP_933847.1 119898634 4608438 complement(2580648..2581226) 1 NC_008702.1 Conserved hypothetical bacterioferritin comigratory protein homolog. Homology to bp1307 of B. pertussis of 43% (trembl|Q7VYL2). Pfam: AhpC/TSA family. signal peptide. no TMHs; Family membership; bacterioferritin comigratory protein 2581226 bcp3 4608438 bcp3 Azoarcus sp. BH72 bacterioferritin comigratory protein YP_933847.1 2580648 R 62928 CDS YP_933848.1 119898635 4608381 complement(2581238..2581618) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 2581618 4608381 azo2344 Azoarcus sp. BH72 hypothetical protein YP_933848.1 2581238 R 62928 CDS YP_933849.1 119898636 4607367 complement(2581721..2583178) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RB5328 of Rhodopirellula baltica of 47% (trembl|Q7US09(SRS)). Signal Peptide present. No TMH reported Present. Has PF07394, Protein of unknown function (DUF1501);IPR010869;This family contains a number of hypothetical bacterial proteins of unknown function approximately 400 residues long.; Conserved hypothetical protein; hypothetical protein 2583178 4607367 azo2345 Azoarcus sp. BH72 hypothetical protein YP_933849.1 2581721 R 62928 CDS YP_933850.1 119898637 4607368 complement(2583205..2585517) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RB6288 of Rhodopirellula baltica of 30% (trembl|Q7UQJ4(SRS)). No domains predicted. No TMHs. Signal P reporting signal peptide.; Conserved hypothetical protein; hypothetical protein 2585517 4607368 azo2346 Azoarcus sp. BH72 hypothetical protein YP_933850.1 2583205 R 62928 CDS YP_933851.1 119898638 4607369 2586159..2588936 1 NC_008702.1 Yersinia enterocolitica,iron-repressible outer membrane protein, FyuA. FyuA:RECEPTOR FOR THE BACTERIOCIN PESTICIN AND FOR THE SIDEROPHORE YERSINIABACTIN. TonB-dependent outer membrane receptor.; Function unclear; putative TonB-dependent receptor 2588936 fyuA 4607369 fyuA Azoarcus sp. BH72 putative TonB-dependent receptor YP_933851.1 2586159 D 62928 CDS YP_933852.1 119898639 4608850 complement(2588979..2589458) 1 NC_008702.1 Similar to a Rhodanese-related sulfurtransferase,PspE. , PspE:Thiosulphate sulphurtransferase ( 2.8.1.1) is an enzyme which catalyzes the transfer of the sulphane atom of thiosulphate to cyanide, to form sulphite and thiocyanate.Involved in cyanide detoxification., Signal peptide present.,; Function unclear; putative rhodanese sulfurtransferase 2589458 4608850 azo2348 Azoarcus sp. BH72 putative rhodanese sulfurtransferase YP_933852.1 2588979 R 62928 CDS YP_933853.1 119898640 4607370 2589804..2591366 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA0435 of Pseudomonas aeruginosa of 35% (trembl|Q9I682(SRS)). No domains predicted. Signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 2591366 4607370 azo2349 Azoarcus sp. BH72 hypothetical protein YP_933853.1 2589804 D 62928 CDS YP_933854.1 119898641 4607371 complement(2591415..2591855) 1 NC_008702.1 Conserved hypothetical protein. Homology to orf of Desulfovibrio vulgaris of 41% (tremblnew|AAS97780(SRS)). No domain predicted. No TMHs. No signal peptide.; hypothetical protein 2591855 4607371 azo2350 Azoarcus sp. BH72 hypothetical protein YP_933854.1 2591415 R 62928 CDS YP_933855.1 119898642 4607372 2592005..2592502 1 NC_008702.1 Putative arsenate reductase (EC 1.20.4.1) (Arsenical pump modifier),; Family membership; putative arsenate reductase (glutaredoxin) 2592502 arsC1 4607372 arsC1 Azoarcus sp. BH72 putative arsenate reductase (glutaredoxin) YP_933855.1 2592005 D 62928 CDS YP_933856.1 119898643 4608352 2592559..2592918 1 NC_008702.1 Putative regulatory protein arsR-family arsenical resistance operon repressor, smtB from Synechococcus PCC 7942, which acts as a transcriptional repressor of the smtA gene that codes for a metallothionein; cadC, a protein required for cadmium-resistance; and hypothetical protein yqcJ from Bacillus subtilis.; Family membership; ArsR family regulatory protein 2592918 arsR 4608352 arsR Azoarcus sp. BH72 ArsR family regulatory protein YP_933856.1 2592559 D 62928 CDS YP_933857.1 119898644 4608355 2592915..2593328 1 NC_008702.1 Putative arsenate reductase,; Family membership; putative arsenate reductase (glutaredoxin) 2593328 arsC2 4608355 arsC2 Azoarcus sp. BH72 putative arsenate reductase (glutaredoxin) YP_933857.1 2592915 D 62928 CDS YP_933858.1 119898645 4608353 2593338..2594402 1 NC_008702.1 Putative arsenical-resistance protein arsB,; Family membership; putative arsenical-resistance protein 2594402 arsB 4608353 arsB Azoarcus sp. BH72 putative arsenical-resistance protein YP_933858.1 2593338 D 62928 CDS YP_933859.1 119898646 4608351 2594417..2594788 1 NC_008702.1 Arsenical resistance operon trans-acting repressor ArsD,; Family membership; arsenical resistance operon trans-acting repressor 2594788 arsD 4608351 arsD Azoarcus sp. BH72 arsenical resistance operon trans-acting repressor YP_933859.1 2594417 D 62928 CDS YP_933860.1 119898647 4608354 2594802..2596550 1 NC_008702.1 Arsenite-transporting ATPase,; High confidence in function and specificity; arsenite-transporting ATPase 2596550 arsA 4608354 arsA Azoarcus sp. BH72 arsenite-transporting ATPase YP_933860.1 2594802 D 62928 CDS YP_933861.1 119898648 4608350 complement(2596593..2598086) 1 NC_008702.1 Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.-) (Glu-ADT subunit A). Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).; Family membership; amidase 2598086 4608350 azo2357 Azoarcus sp. BH72 amidase YP_933861.1 2596593 R 62928 CDS YP_933862.1 119898649 4607373 complement(2598201..2599088) 1 NC_008702.1 Hypothetical AraC-family transcriptional regulator; AraC family transcriptional regulator 2599088 4607373 azo2358 Azoarcus sp. BH72 AraC family transcriptional regulator YP_933862.1 2598201 R 62928 CDS YP_933863.1 119898650 4607374 complement(2599193..2599573) 1 NC_008702.1 oxaloacetate tautomerase,41% identity to TrEMBL;O32183;YusQ protein [yusQ] [Bacillus subtilis subsp. subtilis str. 168]. Has PF01361;(IPR004370);Tautomerase enzyme;This family includes the enzyme 4-oxalocrotonate tautomerase Q01468 that catalyses the ketonisation of 2-hydroxymuconate to 2-oxo-3-hexenedioate. No Signal peptide or TMH reported present.; Conserved hypothetical protein; oxaloacetate tautomerase 2599573 4607374 azo2359 Azoarcus sp. BH72 oxaloacetate tautomerase YP_933863.1 2599193 R 62928 CDS YP_933864.1 119898651 4607375 2599708..2600517 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 2600517 4607375 azo2360 Azoarcus sp. BH72 LysR family transcriptional regulator YP_933864.1 2599708 D 62928 CDS YP_933865.1 119898652 4607376 complement(2600495..2601667) 1 NC_008702.1 Acetyl esterase:-has an esterase activity. triacetyl glycerol (triacetin) is a substrate of the enzyme. Entry name SWISSPROT:AES_ECOLI Prim. accession # P23872 Identities = 57/229 (24%) InterPro IPR002168; Lipolytic_enzyme. IPR000379; Ser_estrs. Pfam :-Thioesterase Prediction: Non-secretory protein Signal peptide probability: 0.233 Number of predicted TMHs: 0; Family membership; putative acetyl esterase 2601667 4607376 azo2361 Azoarcus sp. BH72 putative acetyl esterase YP_933865.1 2600495 R 62928 CDS YP_933866.1 119898653 4607377 complement(2601814..2603754) 1 NC_008702.1 Putative sensor kinase/response regulator hybrid protein,; Specificity unclear; putative sensor kinase/response regulator hybrid protein 2603754 4607377 azo2362 Azoarcus sp. BH72 putative sensor kinase/response regulator hybrid protein YP_933866.1 2601814 R 62928 CDS YP_933867.1 119898654 4607378 complement(2604007..2605017) 1 NC_008702.1 Sulfate-binding protein precursor, Sbp. 69% Sulphate_bind. IPR005669; Thiosulph_bind.InterPro: Prokaryotic sulfate-/thiosulfate-binding protein. TIGRFAMs:TIGR00971; 3a0106s03; 1. Belongs to the prokaryotic sulfate binding protein family. In other bacteria Sbp is a periplasmic component of an ABC transport system.; High confidence in function and specificity; putative periplasmic sulfate-binding protein 2605017 sbp2 4607378 sbp2 Azoarcus sp. BH72 putative periplasmic sulfate-binding protein YP_933867.1 2604007 R 62928 CDS YP_933868.1 119898655 4609842 complement(2605073..2605756) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bll5801 of B. japonicum of 57% (trembl|Q89I38(SRS)). No domains predicted. Signal P reporting signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 2605756 4609842 azo2364 Azoarcus sp. BH72 hypothetical protein YP_933868.1 2605073 R 62928 CDS YP_933869.1 119898656 4607379 2606215..2606502 1 NC_008702.1 Chaperonin GroES. Homology to groES of B. japonicum of 58% (sprot|CH11_BRAJA). Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. InterPro: Chaperonins cpn10 (10 Kd subunit)(IPR001476) Pfam: chaperonin 10 Kd subunit no signal peptide no TMH; High confidence in function and specificity; chaperonin GroES 2606502 groES2 4607379 groES2 Azoarcus sp. BH72 chaperonin GroES YP_933869.1 2606215 D 62928 CDS YP_933870.1 119898657 4608941 2606556..2608199 1 NC_008702.1 Chaperonin GroEL. Homology to groEL of B. japonicum of 65% (SWISSPROT:CH61_BRAJA). Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). Pfam: TCP-1/cpn60 chaperonin family no TMHs; High confidence in function and specificity; chaperonin GroEL 2608199 groEL3 4608941 groEL3 Azoarcus sp. BH72 chaperonin GroEL YP_933870.1 2606556 D 62928 CDS YP_933871.1 119898658 4608939 complement(2608274..2609170) 1 NC_008702.1 Transcriptional regulator, LysR family,; Function unclear; LysR family transcriptional regulator 2609170 4608939 azo2367 Azoarcus sp. BH72 LysR family transcriptional regulator YP_933871.1 2608274 R 62928 CDS YP_933872.1 119898659 4607380 2609291..2610286 1 NC_008702.1 Conserved hypothetical protein. Homology to RSc3335 of Ralstonia solanacearum of 52% (gnl|keqq|rso:RS02569(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2610286 4607380 azo2368 Azoarcus sp. BH72 hypothetical protein YP_933872.1 2609291 D 62928 CDS YP_933873.1 119898660 4607381 2610283..2610723 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Pflu02000368 of Pseudomonas fluorescens of 34% (gi|48732319|ref|ZP_00266062.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2610723 4607381 azo2369 Azoarcus sp. BH72 hypothetical protein YP_933873.1 2610283 D 62928 CDS YP_933874.1 119898661 4607382 2610763..2611167 1 NC_008702.1 Hypothetical secreted protein. No domains predicted. No TMHs. Signal Peptide present. Weak homology with hits in the database.; hypothetical protein 2611167 4607382 azo2370 Azoarcus sp. BH72 hypothetical protein YP_933874.1 2610763 D 62928 CDS YP_933875.1 119898662 4607383 complement(2611545..2611892) 1 NC_008702.1 30S ribosomal protein S4, truncated. Homology of aa 1 to 108 with rpsD of N. europaea of 67% (sprot|RS4B_NITEU(SRS)). With S5 and S12 plays an important role in translational accuracy (By similarity). Pfam: Ribosomal protein S4/S9 n-terminal domain. no signal peptide. no TMHs; Family membership; partiell 30S ribosomal protein S4, truncated 2611892 4607383 azo2371 Azoarcus sp. BH72 partiell 30S ribosomal protein S4, truncated YP_933875.1 2611545 R 62928 CDS YP_933876.1 119898663 4607384 complement(2611911..2612165) 1 NC_008702.1 Hypothetical secreted protein. No homology with the data bank. No domains predicted. Signal peptide present. No TMHs; hypothetical protein 2612165 4607384 azo2372 Azoarcus sp. BH72 hypothetical protein YP_933876.1 2611911 R 62928 CDS YP_933877.1 119898664 4607385 2612445..2612945 1 NC_008702.1 FecI: belongs to the extracytoplasmic-function (ECF) sigma factor, which represent a subgroup of the sigma 70 family. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Involved in iron metabolism.; High confidence in function and specificity; RNA polymerase ECF-subfamily sigma-70 factor 2612945 fecI 4607385 fecI Azoarcus sp. BH72 RNA polymerase ECF-subfamily sigma-70 factor YP_933877.1 2612445 D 62928 CDS YP_933878.1 119898665 4608754 2612948..2613934 1 NC_008702.1 FecR is involved in regulation of iron dicitrate transport. In the absence of citrate FecR inactivates FecI. FecR is probably a sensor that recognizes iron dicitrate in the periplasm. Involved in iron metabolism.; High confidence in function and specificity; putative transmembrane sensor 2613934 fecR 4608754 fecR Azoarcus sp. BH72 putative transmembrane sensor YP_933878.1 2612948 D 62928 CDS YP_933879.1 119898666 4608755 2614041..2616434 1 NC_008702.1 Ferric-citrate outer membrane receptor,FecA.Involved in the recognition and internalization of ferric-citrate in E.coli. SPECIFIC RECEPTOR FOR THE SIDEROPHORE FERRIC PSEUDOBACTIN 358. InterPro: TonB-dependent receptor protein; High confidence in function and specificity; putative ferric-citrate TonB-dependent receptor 2616434 fecA 4608755 fecA Azoarcus sp. BH72 putative ferric-citrate TonB-dependent receptor YP_933879.1 2614041 D 62928 CDS YP_933880.1 119898667 4608753 2616506..2617735 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE0537 of Nitrosomonas europaea of 40% (trembl|Q82WX0(SRS)). no domains predicted. signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 2617735 4608753 azo2376 Azoarcus sp. BH72 hypothetical protein YP_933880.1 2616506 D 62928 CDS YP_933881.1 119898668 4607386 complement(2618005..2618457) 1 NC_008702.1 Similar to Rhodanese, a sulphurtransferase involved in cyanide detoxification. Non-secretory protein. Probable Hypothetical protein RP600.; Function unclear; hypothetical protein 2618457 4607386 azo2377 Azoarcus sp. BH72 hypothetical protein YP_933881.1 2618005 R 62928 CDS YP_933882.1 119898669 4607387 2618632..2619291 1 NC_008702.1 Hypothetical protein, 38% identity (50% similarity) to TrEMBL;Q87X35 Has PF04336, Protein of unknown function,DUF479;IPR007431,This family includes several bacterial proteins of uncharacterised function.; Function unclear; hypothetical protein 2619291 4607387 azo2378 Azoarcus sp. BH72 hypothetical protein YP_933882.1 2618632 D 62928 CDS YP_933883.1 119898670 4607388 complement(2619273..2620175) 1 NC_008702.1 Hypothetical protein, 44% identity (54% similarity) to TrEMBL;Q82TM9. Has PF01636;Phosphotransferase enzyme family;IPR002575,APH_trans;This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include:-aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. Has PF03881,Fructosamine kinase;This family includes eukaryotic fructosamine-3-kinase enzymes. The family also includes bacterial members that have not been characterised but probably have a similar or identical function.; Specificity unclear; hypothetical protein 2620175 4607388 azo2379 Azoarcus sp. BH72 hypothetical protein YP_933883.1 2619273 R 62928 CDS YP_933884.1 119898671 4607389 2620360..2621466 1 NC_008702.1 Glycosyltransferase; Specificity unclear; glycosyltransferase 2621466 4607389 azo2380 Azoarcus sp. BH72 glycosyltransferase YP_933884.1 2620360 D 62928 CDS YP_933885.1 119898672 4607390 2621450..2622997 1 NC_008702.1 Conserved hypothetical protein. Extremely weak homology with hits in the Database spanning the entire length of protein. Has PF02366, Dolichyl-phosphate-mannose-protein mannosyltransferase; IPR003342,Glyco_trans_39:This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferases. These proteins are responsible for O-linked glycosylation of proteins, they catalyse the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Q94891 Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to STT3. TMHMM2 reporting 2 TMH's present. Signal peptide not present.; Function unclear; dolichyl-phosphate-mannose-protein mannosyltransferase 2622997 4607390 azo2381 Azoarcus sp. BH72 dolichyl-phosphate-mannose-protein mannosyltransferase YP_933885.1 2621450 D 62928 CDS YP_933886.1 119898673 4607391 2623216..2624124 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to TdenA01002104 of Thiobacillus denitrificans of 56% (gi|52006510|ref|ZP_00333890.1|(NBCI ENTREZ)). No domains predicted. Signal P repoting a signal peptide present. NO TMH being present.; Conserved hypothetical protein; hypothetical protein 2624124 4607391 azo2382 Azoarcus sp. BH72 hypothetical protein YP_933886.1 2623216 D 62928 CDS YP_933887.1 119898674 4607392 2624136..2624690 1 NC_008702.1 Conserved hypothetical protein. Homology to rsp0720 of R. solanacearum of 43% (trembl|Q8XRW1(SRS)). Pfam: FMN_binding domain. This conserved region includes the FMN-binding site of the NqrC protein as well as the NosR and NirI regulatory proteins. Siganl peptide. no TMHs; hypothetical protein 2624690 4607392 azo2383 Azoarcus sp. BH72 hypothetical protein YP_933887.1 2624136 D 62928 CDS YP_933888.1 119898675 4607393 2624734..2625561 1 NC_008702.1 Conserved hypothetical ApbE family protein. Homology to so1090 of S. oneidensis of 43% (trembl|Q8EHX0). Pfam: ApbE family. This prokaryotic family of lipoproteins are related to ApbE from Salmonella typhimurium. ApbE is involved in thiamine synthesis. no signal. peptide no TMH; Family membership; ApbE family protein 2625561 4607393 azo2384 Azoarcus sp. BH72 ApbE family protein YP_933888.1 2624734 D 62928 CDS YP_933889.1 119898676 4607394 2625563..2626036 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein to the data bank. No domains predicted. signal peptide. 3 TMHs; hypothetical protein 2626036 4607394 azo2385 Azoarcus sp. BH72 hypothetical protein YP_933889.1 2625563 D 62928 CDS YP_933890.1 119898677 4607395 2626263..2626706 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BB4221 of Bordetella bronchiseptica of 32% (trembl|Q7WFQ0). Has PF07331, Protein of unknown function (DUF1468);IPR009936;This family consists of several hypothetical bacterial proteins of around 150 residues in length. The function of this family is unknown. Signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 2626706 4607395 azo2386 Azoarcus sp. BH72 hypothetical protein YP_933890.1 2626263 D 62928 CDS YP_933891.1 119898678 4607396 2626714..2628201 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to an orf of R. palustris of 63% (tremblnew|CAE27761(SRS)). Has PF01970, Integral membrane protein DUF112;IPR002823:Members of this prokaryotic family have no known function. no signal peptide. 10 TMHs; Conserved hypothetical protein; hypothetical protein 2628201 4607396 azo2387 Azoarcus sp. BH72 hypothetical protein YP_933891.1 2626714 D 62928 CDS YP_933892.1 119898679 4607397 2628235..2628873 1 NC_008702.1 Hypothetical membrane protein. no homology of the entire protein to the data bank. no domain predicted. no signal peptide. 4 TMHs; hypothetical protein 2628873 4607397 azo2388 Azoarcus sp. BH72 hypothetical protein YP_933892.1 2628235 D 62928 CDS YP_933893.1 119898680 4607398 complement(2628910..2629125) 1 NC_008702.1 Probable Molybdenum-pterin binding protein II. Organosulfonate utilization protein SsuF InterPro: Molybdenum-pterin binding domain TIGRFAM: Mop: molybdenum-pterin binding domain; High confidence in function and specificity; molybdenum-pterin-binding protein 2629125 ssuF 4607398 ssuF Azoarcus sp. BH72 molybdenum-pterin-binding protein YP_933893.1 2628910 R 62928 CDS YP_933894.1 119898681 4609900 complement(2629358..2629729) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001711 of Dechloromonas aromatica of 36% (gi|53730477|ref|ZP_00348804.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2629729 4609900 azo2390 Azoarcus sp. BH72 hypothetical protein YP_933894.1 2629358 R 62928 CDS YP_933895.1 119898682 4607399 2630041..2631066 1 NC_008702.1 Part of the ABC transporter complex tauABC involved in taurine as a sulfur source import. Similar to the putative aliphatic sulfonates periplasmic-binding protein tauA in E.coli. Probably also involved in nitrate and bicarbonate transport.; Function unclear; putative sulfonate transport system periplasmic component 2631066 tauA 4607399 tauA Azoarcus sp. BH72 putative sulfonate transport system periplasmic component YP_933895.1 2630041 D 62928 CDS YP_933896.1 119898683 4609921 2631101..2631892 1 NC_008702.1 Part of the ABC transporter complex tauABC involved in taurine import as a sulfur source.Probably responsible for energy coupling to the transport system.Similar to the aliphatic sulfonate transport membrane protein, tauB in E.coli. Probably also involved in nitrate and bicarbonate transport.; High confidence in function and specificity; putative sulfonate transport system ATP-binding protein 2631892 tauB 4609921 tauB Azoarcus sp. BH72 putative sulfonate transport system ATP-binding protein YP_933896.1 2631101 D 62928 CDS YP_933897.1 119898684 4609922 2632087..2632899 1 NC_008702.1 Part of the ABC transporter complex tauABC involved in taurine import as sulfur source. Probably responsible for the translocation of the substrate across the membrane.Similar to the aliphatic sulfonate permease, tauC in E.coli. Probably also involved in nitrate and bicarbonate transport.; High confidence in function and specificity; putative sulfonate transport system permease 2632899 tauC 4609922 tauC Azoarcus sp. BH72 putative sulfonate transport system permease YP_933897.1 2632087 D 62928 CDS YP_933898.1 119898685 4609923 2632917..2633645 1 NC_008702.1 Hypothetical membrane protein. Homology to COG2143 from 114 aa to 228 aa. InterPro: Thioredoxin (IPR006662). no signal peptide. 1 TMH; hypothetical protein 2633645 4609923 azo2394 Azoarcus sp. BH72 hypothetical protein YP_933898.1 2632917 D 62928 CDS YP_933899.1 119898686 4607400 2633703..2635040 1 NC_008702.1 Phenylacetate-coenzyme A ligase (PA-CoA ligase).Coenzyme F390 synthase. CATALYZES THE ACTIVATION OF PHENYLACETIC ACID TO PHENYLACETYL-COA. InterPro: AMP-dependent synthetase and ligase; High confidence in function and specificity; phenylacetate--CoA ligase 2635040 phaE 4607400 phaE Azoarcus sp. BH72 phenylacetate--CoA ligase YP_933899.1 2633703 D 62928 CDS YP_933900.1 119898687 4609536 2635527..2637713 1 NC_008702.1 Putative outer membrane TonB-dependent receptor protein. 43% TonB_receptor. Pfam:PF00593; TonB_dep_Rec; 1.; Function unclear; putative TonB-dependent receptor 2637713 4609536 azo2396 Azoarcus sp. BH72 putative TonB-dependent receptor YP_933900.1 2635527 D 62928 CDS YP_933901.1 119898688 4607401 complement(2637720..2638544) 1 NC_008702.1 Conserved hypothetical signaling protein,; Conserved hypothetical protein; putative signaling protein 2638544 4607401 azo2397 Azoarcus sp. BH72 putative signaling protein YP_933901.1 2637720 R 62928 CDS YP_933902.1 119898689 4607402 2638757..2639365 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc1344 of R. solanacearum of 42% (trembl|Q8XZQ1). no domains predicted. no signal peptide. no TMHs; hypothetical protein 2639365 4607402 azo2398 Azoarcus sp. BH72 hypothetical protein YP_933902.1 2638757 D 62928 CDS YP_933903.1 119898690 4607403 2639458..2640438 1 NC_008702.1 Cysteine synthase A,; High confidence in function and specificity; cysteine synthase A 2640438 cysK 4607403 cysK Azoarcus sp. BH72 cysteine synthase A YP_933903.1 2639458 D 62928 CDS YP_933904.1 119898691 4608560 2640828..2643044 1 NC_008702.1 Putative TonB outer membrane receptor protein.31% TonB_boxC. Pfam:PF00593; TonB_dep_Rec; 1. Signal peptide present.; Function unclear; putative TonB-dependent receptor protein 2643044 4608560 azo2400 Azoarcus sp. BH72 putative TonB-dependent receptor protein YP_933904.1 2640828 D 62928 CDS YP_933905.1 119898692 4607404 2643054..2643623 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsp0812 of R. solanacearum of 30% (trembl|Q8XRL9). no damians predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2643623 4607404 azo2401 Azoarcus sp. BH72 hypothetical protein YP_933905.1 2643054 D 62928 CDS YP_933906.1 119898693 4607405 2643620..2646658 1 NC_008702.1 Putative sensor-regulator protein,; Specificity unclear; putative sensor-regulator protein 2646658 barA 4607405 barA Azoarcus sp. BH72 putative sensor-regulator protein YP_933906.1 2643620 D 62928 CDS YP_933907.1 119898694 4608376 2646655..2647560 1 NC_008702.1 Probable two-component response regulator,; High confidence in function and specificity; two-component response regulator 2647560 wspR2 4608376 wspR2 Azoarcus sp. BH72 two-component response regulator YP_933907.1 2646655 D 62928 CDS YP_933908.1 119898695 4610116 2647703..2649856 1 NC_008702.1 Hypothetical secreted protein. no homology of the entire protein to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 2649856 4610116 azo2404 Azoarcus sp. BH72 hypothetical protein YP_933908.1 2647703 D 62928 CDS YP_933909.1 119898696 4607406 complement(2649868..2650305) 1 NC_008702.1 Probable organic hydroperoxide resistance protein. Homology to ohr of X. campestris of 50% (SWISSPROT:OHR_XANAC). Organic hydroperoxide detoxification protein. Confers increased resistance to tert-butyl hydroperoxide killing. InterPro: OsmC-like protein (IPR003718) Pfam: OsmC-like protein no signal peptide no TMH; High confidence in function and specificity; organic hydroperoxide resistance protein 2650305 ohr 4607406 ohr Azoarcus sp. BH72 organic hydroperoxide resistance protein YP_933909.1 2649868 R 62928 CDS YP_933910.1 119898697 4609440 complement(2650430..2654014) 1 NC_008702.1 catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates; 2-oxoacid ferredoxin oxidoreductase 2654014 4609440 azo2406 Azoarcus sp. BH72 2-oxoacid ferredoxin oxidoreductase YP_933910.1 2650430 R 62928 CDS YP_933911.1 119898698 4607407 2654233..2654715 1 NC_008702.1 Bkd operon transcriptional regulator, similarity to SWISSPROT: (38% Pseudomonas putida, bkd operon transcriptional regulator BdkR) InterPro: IPR000485 HTH_AsnC_lrp. Pfam: PF01037 AsnC family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; bkd operon transcriptional regulator 2654715 bkdR 4607407 bkdR Azoarcus sp. BH72 bkd operon transcriptional regulator YP_933911.1 2654233 D 62928 CDS YP_933912.1 119898699 4608397 2654882..2657899 1 NC_008702.1 Hypothetical sensor protein,; Conserved hypothetical protein; sensor protein 2657899 4608397 azo2408 Azoarcus sp. BH72 sensor protein YP_933912.1 2654882 D 62928 CDS YP_933913.1 119898700 4607408 complement(2657913..2659973) 1 NC_008702.1 Conserved hypothetical sodium:solute sympoter. Homology to bb3915 of B. bronchiseptica of 57% (trembl|Q7WCM0). Sodium/substrate symport (or co-transport) is a widespread mechanism of solute transport across cytoplasmic membranes of pro- and eukaryotic cells. Thereby the energy stored in an inwardly directed electrochemical sodium gradient (sodium motive force, SMF) is used to drive solute accumulation against a concentration gradient. The solutes transported may be sugars, amino acids, nucleosides, inositols, vitamins,urea or anions, depending on the system. Tigrfam: sss: SSS sodium solute transporter superfamily. Pfam: Sodium:solute symporter family. signal peptide. 13 TMHS; Family membership; putative sodium:solute sympoter 2659973 4607408 azo2409 Azoarcus sp. BH72 putative sodium:solute sympoter YP_933913.1 2657913 R 62928 CDS YP_933914.1 119898701 4607409 complement(2660014..2662218) 1 NC_008702.1 DNA polymerase III epsilon chain-like protein (EC 2.7.7.7). DNA POLYMERASE III IS A COMPLEX MULTICHAIN ENZYME RESPONSIBLE FOR MOST OF THE REPLICATIVE SYNTHESIS IN BACTERIA. THE EPSILON SUBUNIT CONTAIN THE EDITING FUNCTION AND IS A PROOFREADING 3-5 EXONUCLEASE (BY SIMILARITY). dnaq: DNA polymerase III epsilon sub; Family membership; DNA polymerase III subunit epsilon 2662218 4607409 azo2410 Azoarcus sp. BH72 DNA polymerase III subunit epsilon YP_933914.1 2660014 R 62928 CDS YP_933915.1 119898702 4607410 complement(2662346..2662720) 1 NC_008702.1 Transcriptional regulatory protein yycF. Member of the two-component regulatory system yycG/yycF involved in the regulation of the ftsAZ operon. Binds to the ftsAZ P1 promoter sequence. Similar to SWISSPROT: sprot|YYCF_BACSU (50% Bacillus subtilis, transcriptional regulatory protein YycF) / sprot|PHOP_BACSU (47% Bacillus subtilis, alkaline phosphatase synthesis transcriptional regulatory protein PhoP) InterPro: IPR001789 Response_reg. Pfam: PF00072 Response_reg.; Specificity unclear; transcriptional regulator 2662720 yycF 4607410 yycF Azoarcus sp. BH72 transcriptional regulator YP_933915.1 2662346 R 62928 CDS YP_933916.1 119898703 4610200 complement(2662752..2665499) 1 NC_008702.1 Sensor histidine kinase mtrB (EC 2.7.3.-). Member of the two-component regulatory system mtrA/mtrB. Seems to function as a membrane-associated protein kinase that phosphorylates mtrA in response to environmental signals (By similarity). TREMBLnew:AAS80811; TREMBL:Q8PEZ5, 42% identity, 59% similarity; Pfam: Histidine kinase _,Phosphoacceptor domain. TIGRFAM: sss: sodium solute transporter sup. TMHMM predicted transmembrane helices. sss: SSS sodium solute transporter sup; Specificity unclear; two-component system sensor protein 2665499 4610200 azo2412 Azoarcus sp. BH72 two-component system sensor protein YP_933916.1 2662752 R 62928 CDS YP_933917.1 119898704 4607411 complement(2665499..2665696) 1 NC_008702.1 Conserved hypothetical membrane protein. Homolgy to ttc0464 of T. thermophilus of 46% (tremble:Q72K63). no domains predicted. singal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2665696 4607411 azo2413 Azoarcus sp. BH72 hypothetical protein YP_933917.1 2665499 R 62928 CDS YP_933918.1 119898705 4607412 complement(2665962..2667926) 1 NC_008702.1 Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA; acetyl-CoA synthetase 2667926 acsB 4607412 acsB Azoarcus sp. BH72 acetyl-CoA synthetase YP_933918.1 2665962 R 62928 CDS YP_933919.1 119898706 4608275 2668208..2669899 1 NC_008702.1 Putative fumarate hydratase. Homology to fumB of E. coli of 25% (sprot|FUMB_ECOLI). It functions in the generation of fumarate for use as an anaerobic electron acceptor. Tigrfam: ttdA_fumA_fumB: hydro-lyases Fe-S type,tartrate/fumarate subfamily, alpha region; ttdB_fumA_fumB: hydro-lyases Fe-S type, tartrate/fumarate subfamily, beta region no signal peptide no TMHs; Family membership; putative fumarate hydratase 2669899 fumB 4608275 fumB Azoarcus sp. BH72 putative fumarate hydratase YP_933919.1 2668208 D 62928 CDS YP_933920.1 119898707 4608845 complement(2669865..2670155) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2670155 4608845 azo2416 Azoarcus sp. BH72 hypothetical protein YP_933920.1 2669865 R 62928 CDS YP_933921.1 119898708 4607413 complement(2670306..2671496) 1 NC_008702.1 NnrS is a putative heme-Cu protein and a member of the short-chain dehydrogenase family, probably involved in the nitric oxide metabolism. Genes encoding putative orthologues of NnrS are sometimes but not always found in bacteria encoding nitrite and/or nitric oxide reductase. 40% NnrS. TMhelix:10 Pfam:PF05940; NnrS; 1.; Function unclear; putative heme-Cu-containing membrane protein 2671496 nnrS 4607413 nnrS Azoarcus sp. BH72 putative heme-Cu-containing membrane protein YP_933921.1 2670306 R 62928 CDS YP_933922.1 119898709 4609387 2671716..2672762 1 NC_008702.1 D-erythrose 4-phosphate dehydrogenase(E4PDH), GapB protein. Could play a role in pyridoxal 5-phosphate synthesis.Involved in the reaction: d-erythrose 4-phosphate + nad(+) = 4-phosphoerythronate + nadh. 51% E4PD_g-proteo.IPR000173; GAP_dhdrogenase. Pfam:PF00044; gpdh; 1.PF02800; gpdh_C; 1. TIGR00274: glucokinase regulator-relate. TIGRFAMs:TIGR01532; E4PD_g-proteo; 1.; High confidence in function and specificity; putative D-erythrose 4-phosphate dehydrogenase 2672762 gapB 4609387 gapB Azoarcus sp. BH72 putative D-erythrose 4-phosphate dehydrogenase YP_933922.1 2671716 D 62928 CDS YP_933923.1 119898710 4608859 complement(2672763..2673671) 1 NC_008702.1 LysR-type transcriptional regulator NahR. Regulates the expression of the naphthalene (nahA-F) and salicylate (nahG-M) metabolism genes. Belongs to the LysR family of transcriptional regulators. Similar to SWISSPROT: sprot|NAHR_PSEPU (40% Pseudomonas putida, hth-type transcriptional activator NahR) Pfam: PF00126 Bacterial regulatory helix-turn-helix protein, lysR family. HTH reporting nucleic acid binding motif.; Specificity unclear; putative LysR-type transcriptional regulator NahR 2673671 nahR1 4608859 nahR1 Azoarcus sp. BH72 putative LysR-type transcriptional regulator NahR YP_933923.1 2672763 R 62928 CDS YP_933924.1 119898711 4609324 2673841..2674878 1 NC_008702.1 Involved in catabolism of naphthalene via gentisate to fumarate and pyruvate. NagI is a gentisate 1,2-dioxygenase which converts gentisate to maleylpyruvate. Similar to TREMBL:O86041 (66% identity); TREMBL:Q8X655 (30% identity); TREMBL:Q8Y0F7 (33% identity).; High confidence in function and specificity; gentisate 1,2-dioxygenase 2674878 nagI 4609324 nagI Azoarcus sp. BH72 gentisate 1,2-dioxygenase YP_933924.1 2673841 D 62928 CDS YP_933925.1 119898712 4609311 2674933..2675643 1 NC_008702.1 Involved in catabolism of naphthalene via gentisate to fumarate and pyruvate. NagK is a fumarylpyruvate hydrolase which hydrolyzes fumarylpyruvate to fumarate and pyruvate. Similar to TREMBL:O86042 (67% identity); TREMBL:O07877 (63% identity); TREMBL:Q8G387 (50% identity). Pfam (PF01557): Fumarylacetoacetate (FAA) hydrolase family.; High confidence in function and specificity; fumarylpyruvate hydrolase 2675643 nagK 4609311 nagK Azoarcus sp. BH72 fumarylpyruvate hydrolase YP_933925.1 2674933 D 62928 CDS YP_933926.1 119898713 4609312 2675686..2676879 1 NC_008702.1 catalyzes the formation of catechol from salicylate; salicylate hydroxylase 2676879 nahG 4609312 nahG Azoarcus sp. BH72 salicylate hydroxylase YP_933926.1 2675686 D 62928 CDS YP_933927.1 119898714 4609317 2676985..2677626 1 NC_008702.1 maleylpyruvate isomerase. Homology to nogL of Ralstonia sp U of 73% (AAD12621) NagL is a reduced glutathione-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate. InterPro: Glutathione S-transferase N terminus (IPR004045) Pfam: Glutathione S-transferase, N-terminale domaine no signal peptide no TMHs; High confidence in function and specificity; maleylpyruvate isomerase 2677626 nagL1 4609317 nagL1 Azoarcus sp. BH72 maleylpyruvate isomerase YP_933927.1 2676985 D 62928 CDS YP_933928.1 119898715 4609313 2677699..2678685 1 NC_008702.1 TRAP-dicarboxylate transporter. Binds c4-dicarboxylates; part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. 23% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide: present.; Specificity unclear; putative C4-dicarboxylate-binding periplasmic protein 2678685 dctP3 4609313 dctP3 Azoarcus sp. BH72 putative C4-dicarboxylate-binding periplasmic protein YP_933928.1 2677699 D 62928 CDS YP_933929.1 119898716 4608604 2679037..2679540 1 NC_008702.1 TRAP-type C4-dicarboxylate transport system, small permease component. The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 22% DctM.IPR007387; DctQ.IPR000252; DedA.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF04290; DctQ; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1.; Specificity unclear; putative TRAP-type C4-dicarboxylate transport system small permease 2679540 dctQ4 4608604 dctQ4 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system small permease YP_933929.1 2679037 D 62928 CDS YP_933930.1 119898717 4608611 2679537..2680814 1 NC_008702.1 Similar to a putative large integral C4-dicarboxylate membrane transport protein from W. succinogenes.DctM: TRAP dicarboxylate transporter. TREMBL:Q9ZEJ2 InterPro:IPR010656; DctM.IPR000252; DedA.IPR000524; HTH_GntR.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1. TMHelix: 12. Signal peptide:present.; Specificity unclear; putative TRAP-type C4-dicarboxylate transport system large permease 2680814 dctM4 4608611 dctM4 Azoarcus sp. BH72 putative TRAP-type C4-dicarboxylate transport system large permease YP_933930.1 2679537 D 62928 CDS YP_933931.1 119898718 4608600 complement(2680831..2681421) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BB1720 of Bordetella bronchiseptica of 32% (trembl|Q7WLM5). Has PF03992, Antibiotic biosynthesis monooxygenase;IPR007138;This domain is found in monooxygenases involved in the biosynthesis of several antibiotics by Streptomyces species. It's occurrence as a repeat in Streptomyces coelicolor SCO1909 (Q9X9W3) is suggestive that the other proteins function as multimers. There is also a conserved histidine which is likely to be an active site residue. TMHMM2 reporting 2 TMH's Present. No Signal Peptide present.; Conserved hypothetical protein; hypothetical protein 2681421 4608600 azo2427 Azoarcus sp. BH72 hypothetical protein YP_933931.1 2680831 R 62928 CDS YP_933932.1 119898719 4607414 complement(2681551..2682474) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to an orf of T. aromatica of 48% (trembl|Q7B0A2). no domains. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2682474 4607414 azo2428 Azoarcus sp. BH72 hypothetical protein YP_933932.1 2681551 R 62928 CDS YP_933933.1 119898720 4607415 complement(2682655..2682846) 1 NC_008702.1 4-oxalocrotonate tautomerase (EC 5.3.2.-) (4-OT). Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate. SPROT:Q9RHM8: 80% identity; 88% similarity InterPro: 4-oxalocrotonate tautomerase InterPro:IPR004370; Taut. ProDom: PD404143; Taut Pfam; PF01361; Tautomerase; 1. taut: 4-oxalocrotonate tautomerase helixturnhelix: reporting nucleic acid binding motif No transmembrane helices; High confidence in function and specificity; 4-oxalocrotonate tautomerase 2682846 nahJ 4607415 nahJ Azoarcus sp. BH72 4-oxalocrotonate tautomerase YP_933933.1 2682655 R 62928 CDS YP_933934.1 119898721 4609319 complement(2683055..2683843) 1 NC_008702.1 Similar to TREMBL:Q93JW1 (67% identity); TREMBL:Q9RHN0 (67% identity); SWISSPROT:P49155 (59% identity). Pfam (PF01689): Hydratase/decarboxylase.; High confidence in function and specificity; 4-oxalocrotonate decarboxylase 2683843 nahK 4609319 nahK Azoarcus sp. BH72 4-oxalocrotonate decarboxylase YP_933934.1 2683055 R 62928 CDS YP_933935.1 119898722 4609320 complement(2683840..2684880) 1 NC_008702.1 catalyzes the formation of pyruvate and acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved in the degradation of phenylpropionate; 4-hydroxy-2-ketovalerate aldolase 2684880 nahM 4609320 nahM Azoarcus sp. BH72 4-hydroxy-2-ketovalerate aldolase YP_933935.1 2683840 R 62928 CDS YP_933936.1 119898723 4609322 complement(2684895..2685815) 1 NC_008702.1 catalyzes the formation of acetyl-CoA from acetalaldehyde; acetaldehyde dehydrogenase 2685815 nahO 4609322 nahO Azoarcus sp. BH72 acetaldehyde dehydrogenase YP_933936.1 2684895 R 62928 CDS YP_933937.1 119898724 4609323 complement(2685848..2686642) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q8XUP8 (61% identity); TREMBL:Q9I400 (57% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR) superfamily. InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 2686642 4609323 azo2433 Azoarcus sp. BH72 short-chain dehydrogenase YP_933937.1 2685848 R 62928 CDS YP_933938.1 119898725 4607416 complement(2686737..2687519) 1 NC_008702.1 Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy-2-oxopentanoate. Meta-cleavage pathway for the degradation of phenols, cresols and catechol. Belongs to the todj/xyli/xylj/hpcg family. Similar to TREMBL:Q93JW5 (75% identity); TREMBL:Q9ZI58 (65% identity); SWISSPROT:P23107 (73% identity). InterPro (IPR002607): Hydratase/decarboxylase Pfam (PF01689): Hydratase/decarboxylase.; High confidence in function and specificity; 2-oxopent-4-enoate hydratase 2687519 nahL 4607416 nahL Azoarcus sp. BH72 2-oxopent-4-enoate hydratase YP_933938.1 2686737 R 62928 CDS YP_933939.1 119898726 4609321 complement(2687635..2689089) 1 NC_008702.1 2-hydroxymuconic semialdehyde dehydrogenase (HMSD). Homology to nahI of P. stutzeri of 73% (TREMBL:Q9S602) 2-HYDROXYMUCONIC ACID SEMIALDEHYDE CAN BE CONVERTED TO 2-HYDROXYPENT-24-DIENOATE EITHER DIRECTLY BY THE ACTION OF 2-HYDROXYMUCONIC SEMIALDEHYDE HYDROLASE (HMSH) OR BY THE ACTION OF THREE SEQUENTIAL ENZYMES THE FIRST OF WHICH IS HMSD. InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs proA: gamma-glutamyl phosphate reductas; 2-hydroxymuconic semialdehyde dehydrogenase 2689089 nahI 4609321 nahI Azoarcus sp. BH72 2-hydroxymuconic semialdehyde dehydrogenase YP_933939.1 2687635 R 62928 CDS YP_933940.1 119898727 4609318 complement(2689143..2689580) 1 NC_008702.1 Conserved hypothetical protein. Homology to orf4 of P. putida of 59% (trembl|Q93JW8). Pfam: DUF336. This family contains uncharacterised sequences, including several GlcG proteins. The alignment contains many conserved motifs that are suggestive of cofactor binding and enzymatic activity. no signal peptide. no TMHs; hypothetical protein 2689580 4609318 azo2436 Azoarcus sp. BH72 hypothetical protein YP_933940.1 2689143 R 62928 CDS YP_933941.1 119898728 4607417 2689738..2690457 1 NC_008702.1 Transcriptional regulator,; High confidence in function and specificity; transcriptional regulator 2690457 aphS 4607417 aphS Azoarcus sp. BH72 transcriptional regulator YP_933941.1 2689738 D 62928 CDS YP_933942.1 119898729 4608327 complement(2690810..2691739) 1 NC_008702.1 In Pseudomonas sp. strain KL28 the genes,designated as lap (for long-chain alkylphenols), encoding enzymes for the catabolic 3- and 4-alkylphenol degradation pathway. Catechol 2,3-dioxygenase LapB,; High confidence in function and specificity; catechol 2,3-dioxygenase 2691739 lapB2 4608327 lapB2 Azoarcus sp. BH72 catechol 2,3-dioxygenase YP_933942.1 2690810 R 62928 CDS YP_933943.1 119898730 4609110 complement(2691869..2692924) 1 NC_008702.1 Probable phenol hydroxylase (EC 1.14.13.7) (Phenol 2-monooxygenase P5 component). Probable electron transfer from NADPH via FAD and the 2Fe-2S center to the oxygenase activity site of the enzyme. InterPro: Oxidoreductase FAD and NAD(P)-binding domain hisT_truA: tRNA pseudouridine synthase A; Specificity unclear; phenol hydroxylase subunit P5 2692924 poxF 4609110 poxF Azoarcus sp. BH72 phenol hydroxylase subunit P5 YP_933943.1 2691869 R 62928 CDS YP_933944.1 119898731 4609604 complement(2693134..2693493) 1 NC_008702.1 Pseudomonas sp. phenol hydroxylase P4 protein (EC 1.14.13.7, (phenol 2-monooxygenase P4 component). CATABOLIZES PHENOL AND SOME OF ITS METHYLATED DERIVATIVES. P4 IS REQUIRED FOR GROWTH ON PHENOL AND FOR IN VITRO PHENOL HYDROXYLASE ACTIVITY.; High confidence in function and specificity; phenol hydroxylase subunit P4 2693493 poxE 4609604 poxE Azoarcus sp. BH72 phenol hydroxylase subunit P4 YP_933944.1 2693134 R 62928 CDS YP_933945.1 119898732 4609603 complement(2693966..2695519) 1 NC_008702.1 Probable phenol hydroxylase subunit,68% identity(84% similarity) to trEMBL:Q9RAF6,Phenol hydroxylase component [phyC] [Ralstonia sp. KN1]. TrEMBL;Q9ZNP4 Has PF02332:Methane/Phenol/Toluene Hydroxylase;IPR003430;Bacterial phenol hydroxylase (EC: 1.14.13.7) is a multicomponent enzyme that catabolises phenol and some of its methylated derivatives. This family contains both the P1 and P3 polypeptides of phenol hydroxlase and the alpha and beta chain of methane hydroxylase A. Methane hydroxylase A (EC: 1.14.13.25) is responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyses the monohydroxylation of a variety of unactivated alkenes,alicyclic, aromatic and heterocyclic compounds. Also included in this family is toluene-4-monooxygenase system protein A (EC: 1.14.13.-), which hydroxylates toluene to form P-cresol. PF04945:IPR007029; YHS domain; This short presumed domain is about 50 amino acid residues long. It often contains two cysteines that may be functionally important. This domain is found in copper transporting ATPases, some phenol hydroxylases and in a set of uncharacterised membrane proteins including Q9CNI0. This domain is named after three of the most conserved amino acids it contains. The domain may be metal binding,possibly copper ions. This domain is duplicated in some copper transporting ATPases.; High confidence in function and specificity; phenol hydroxylase subunit P3 2695519 poxD 4609603 poxD Azoarcus sp. BH72 phenol hydroxylase subunit P3 YP_933945.1 2693966 R 62928 CDS YP_933946.1 119898733 4609602 complement(2695548..2695817) 1 NC_008702.1 Probable phenol hydrolase, subunit P2. Catabolizes phenol and some of its methylated derivatives. p2 is required for growth on phenol, and for in vitro phenol hydroxylase activity. Similar to SWISSPROT:P19731 (47% similarity,71% identity). Pfam (PF02406): MmoB/DmpM family.; High confidence in function and specificity; phenol hydrolase subunit P2 2695817 poxC 4609602 poxC Azoarcus sp. BH72 phenol hydrolase subunit P2 YP_933946.1 2695548 R 62928 CDS YP_933947.1 119898734 4609601 complement(2695828..2696823) 1 NC_008702.1 Probable Phenol hydroxylase, subunit P1,65% identity(69% similarity) to TrEMBL;Q7WYF3 LapL [lapL] [Pseudomonas sp. KL28]. Has PF02332;Methane/Phenol/Toluene Hydroxylase domain;IPR003430: Bacterial phenol hydroxylase (EC: 1.14.13.7) is a multicomponent enzyme that catabolises phenol and some of its methylated derivatives. This family contains both the P1 and P3 polypeptides of phenol hydroxlase and the alpha and beta chain of methane hydroxylase A. Methane hydroxylase A (EC: 1.14.13.25) is responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyses the monohydroxylation of a variety of unactivated alkenes,alicyclic, aromatic and heterocyclic compounds. Also included in this family is toluene-4-monooxygenase system protein A (EC: 1.14.13.-), which hydroxylates toluene to form P-cresol. No signal peptide or TMH reported to be present.; Family membership; phenol hydroxylase subunit P1 2696823 poxB 4609601 poxB Azoarcus sp. BH72 phenol hydroxylase subunit P1 YP_933947.1 2695828 R 62928 CDS YP_933948.1 119898735 4609600 complement(2696900..2697163) 1 NC_008702.1 Putative phenol hydroxylase, subunit P0. Homology to poxA of R. eutropha of 34% (trembl:O84958) This family consists of several bacterial phenol hydroxylase subunit proteins which are part of a multicomponent phenol hydroxylase. Some bacteria can utilise phenol or some of its methylated derivatives as their sole source of carbon and energy. The first step in this process is the conversion of phenol into catechol. Catechol is then further metabolised via the meta-cleavage pathway into TCA cycle intermediates. Pfam: Phenol hydrolase subunit no signal peptide no TMHs; Family membership; putative phenol hydrolase subunit P0 2697163 poxA 4609600 poxA Azoarcus sp. BH72 putative phenol hydrolase subunit P0 YP_933948.1 2696900 R 62928 CDS YP_933949.1 119898736 4609599 complement(2697463..2697780) 1 NC_008702.1 Probable ferredoxin-like protein. Homology to poxG of R. eutropha of 56% (trembl|O84964) The ferredoxins are iron-sulphur proteins that transfer electrons in a wide variety of metabolic reactions. Pfam: 2Fe-2S iron-sulfur cluster binding domain no signal peptide no TMHs; Family membership; ferredoxin-like protein 2697780 poxG 4609599 poxG Azoarcus sp. BH72 ferredoxin-like protein YP_933949.1 2697463 R 62928 CDS YP_933950.1 119898737 4609605 2698080..2699780 1 NC_008702.1 Transcriptional regulatory protein probable involved into the phenol degradation pathway,; High confidence in function and specificity; positive regulator of phenol-degratives genes 2699780 poxR 4609605 poxR Azoarcus sp. BH72 positive regulator of phenol-degratives genes YP_933950.1 2698080 D 62928 CDS YP_933951.1 119898738 4609606 complement(2699777..2700655) 1 NC_008702.1 pobR. Transcriptional regulator AraC family. InterPro: AraC type helix-turn-helix pdxH: pyridoxamine 5-phosphate oxidase; Function unclear; PobR regulator 2700655 pobR1 4609606 pobR1 Azoarcus sp. BH72 PobR regulator YP_933951.1 2699777 R 62928 CDS YP_933952.1 119898739 4609594 2700822..2702009 1 NC_008702.1 catalyzes the formation of protocatechuate from 4-hydroxybenzoate; 4-hydroxybenzoate 3-monooxygenase 2702009 pobA 4609594 pobA Azoarcus sp. BH72 4-hydroxybenzoate 3-monooxygenase YP_933952.1 2700822 D 62928 CDS YP_933953.1 119898740 4609593 complement(2702082..2703143) 1 NC_008702.1 Putative two component response regulator,; Conserved hypothetical protein; putative two component response regulator 2703143 4609593 azo2449 Azoarcus sp. BH72 putative two component response regulator YP_933953.1 2702082 R 62928 CDS YP_933954.1 119898741 4607418 complement(2703151..2705901) 1 NC_008702.1 Putative two-component system sensor protein,; Function unclear; putative two-component system sensor protein 2705901 4607418 azo2450 Azoarcus sp. BH72 putative two-component system sensor protein YP_933954.1 2703151 R 62928 CDS YP_933955.1 119898742 4607419 2706261..2706971 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q98J95 (48% identity); TREMBL:Q8YZV3 (43% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. PFAM (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 2706971 4607419 azo2451 Azoarcus sp. BH72 short-chain dehydrogenase YP_933955.1 2706261 D 62928 CDS YP_933956.1 119898743 4607420 2707060..2707500 1 NC_008702.1 Conserved hypothetical protein, 31% identity (51% similarity) to TrEMBL;Q6NAA7. Has PF02637, GatB/Yqey domain;IPR003789:This domain is found in GatB and proteins related to bacterial Yqey P54464. It is about 140 amino acid residues long. This domain is found at the C terminus of GatB O30509 which transamidates Glu-tRNA to Gln-tRNA. The function of this domain is uncertain. It does however suggest that Yqey and its relatives have a role in tRNA metabolism. No Signal peptide or TMH present.; Function unclear; hypothetical protein 2707500 4607420 azo2452 Azoarcus sp. BH72 hypothetical protein YP_933956.1 2707060 D 62928 CDS YP_933957.1 119898744 4607421 complement(2707510..2708151) 1 NC_008702.1 Probable negative transcriptional regulator,; Family membership; negative transcriptional regulator 2708151 4607421 azo2453 Azoarcus sp. BH72 negative transcriptional regulator YP_933957.1 2707510 R 62928 CDS YP_933958.1 119898745 4607422 2708500..2709174 1 NC_008702.1 Hypothetical secreted protein no homology of the entire protein to the data bank no domains predicted signal peptide no TMHs; hypothetical protein 2709174 4607422 azo2454 Azoarcus sp. BH72 hypothetical protein YP_933958.1 2708500 D 62928 CDS YP_933959.1 119898746 4607423 complement(2709188..2709628) 1 NC_008702.1 Putative two-component response regulator,; Specificity unclear; putative two-component response regulator 2709628 rcp2 4607423 rcp2 Azoarcus sp. BH72 putative two-component response regulator YP_933959.1 2709188 R 62928 CDS YP_933960.1 119898747 4609697 complement(2709625..2711352) 1 NC_008702.1 Putative two-component system sensor protein,; Function unclear; putative two-component system sensor protein 2711352 cph1 4609697 cph1 Azoarcus sp. BH72 putative two-component system sensor protein YP_933960.1 2709625 R 62928 CDS YP_933961.1 119898748 4608526 complement(2711355..2713823) 1 NC_008702.1 Putative sensory box histidine kinase,; Function unclear; putative sensory box histidine kinase 2713823 4608526 azo2457 Azoarcus sp. BH72 putative sensory box histidine kinase YP_933961.1 2711355 R 62928 CDS YP_933962.1 119898749 4607424 complement(2714039..2714560) 1 NC_008702.1 Putative transcriptional regulator, LuxR family,very low similarity to SWISSPROT: sprot|NARP_ECOLI (16% Escherichia coli, NarP) InterPro: IPR000792 HTH_LuxR. Pfam: PF00196 GerE. Signal P reporting signal peptide. HTH reporting nucleic acid binding motif.; Function unclear; LuxR family transcriptional regulator 2714560 4607424 azo2458 Azoarcus sp. BH72 LuxR family transcriptional regulator YP_933962.1 2714039 R 62928 CDS YP_933963.1 119898750 4607425 2715013..2716176 1 NC_008702.1 Hypothetical protein, has very weak homolgy with hits in the database. NO motifs,domains, signal peptide or TMH present.; hypothetical protein 2716176 4607425 azo2459 Azoarcus sp. BH72 hypothetical protein YP_933963.1 2715013 D 62928 CDS YP_933964.1 119898751 4607426 2716200..2717258 1 NC_008702.1 Conserved hypothetical protein. Homology to mll6891 of Mesorhizobium loti of 37% (gi|14026496|dbj|BAB53093.1|). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2717258 4607426 azo2460 Azoarcus sp. BH72 hypothetical protein YP_933964.1 2716200 D 62928 CDS YP_933965.1 119898752 4607427 2717280..2718590 1 NC_008702.1 Conserved hypothetical protein. Homology to mll6889 of M.loti. of 58% (trembl|Q987V8(SRS)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2718590 4607427 azo2461 Azoarcus sp. BH72 hypothetical protein YP_933965.1 2717280 D 62928 CDS YP_933966.1 119898753 4607428 2718645..2720618 1 NC_008702.1 Hypothetical secreted protein. No homology of the entire protein to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 2720618 4607428 azo2462 Azoarcus sp. BH72 hypothetical protein YP_933966.1 2718645 D 62928 CDS YP_933967.1 119898754 4607429 2720645..2721274 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 2721274 4607429 azo2463 Azoarcus sp. BH72 hypothetical protein YP_933967.1 2720645 D 62928 CDS YP_933968.1 119898755 4607430 2721255..2722364 1 NC_008702.1 Conserved hypothetical protein. Homology to mll6883 of M.loti of 58% (trembl|Q987W1(SRS)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2722364 4607430 azo2464 Azoarcus sp. BH72 hypothetical protein YP_933968.1 2721255 D 62928 CDS YP_933969.1 119898756 4607431 2722381..2723358 1 NC_008702.1 Hypothetical protein. No homology to the data base. No domains predicted. No signal peptide. No TMHs.; hypothetical protein 2723358 4607431 azo2465 Azoarcus sp. BH72 hypothetical protein YP_933969.1 2722381 D 62928 CDS YP_933970.1 119898757 4607432 2723377..2724144 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2724144 4607432 azo2466 Azoarcus sp. BH72 hypothetical protein YP_933970.1 2723377 D 62928 CDS YP_933971.1 119898758 4607433 2724141..2725190 1 NC_008702.1 Conserved hypothetical protein. Homology to mll6881 of M.loti of 49% (trembl|Q987W3(SRS)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2725190 4607433 azo2467 Azoarcus sp. BH72 hypothetical protein YP_933971.1 2724141 D 62928 CDS YP_933972.1 119898759 4607434 2725351..2726754 1 NC_008702.1 Conserved hypothetical transcriptional regulator. Homology to pp3544 of P. putida of 56% (trembl|Q88H21). InterPro: Aminotransferases class-I (IPR004838); Bacterial regulatory proteins, GntR family (IPR000524). Pfam: Bacterial regulatory proteins, gntR family; Aminotransferase class I and II. no signal peptide. no TMHs; Family membership; putative transcriptional regulator 2726754 4607434 azo2468 Azoarcus sp. BH72 putative transcriptional regulator YP_933972.1 2725351 D 62928 CDS YP_933973.1 119898760 4607435 complement(2726765..2727181) 1 NC_008702.1 Conserved hypothetical protein. Homology to bb1298 of B. brochiseptica of 64% (trembl|Q7WMU2). Pfam: Glyoxalase/Bleomycin restiant protein. no signal peptide. no TMHs; hypothetical protein 2727181 4607435 azo2469 Azoarcus sp. BH72 hypothetical protein YP_933973.1 2726765 R 62928 CDS YP_933974.1 119898761 4607436 complement(2727286..2728407) 1 NC_008702.1 Conserved hypothetical L-lactate dehydrogenase (cytochrome). Homology to lldD of B. bronchisptica of 51% (tremble:Q7WND1). InterPro: FMN-dependent alpha-hydroxy acid dehydrogenases (IPR000262); Protein binding FMN and related compounds core region (IPR003009). Pfam: FMN-dependent dehydrognease. no signal peptide. no TMHs; Family membership; L-lactate dehydrogenase 2728407 lldD 4607436 lldD Azoarcus sp. BH72 L-lactate dehydrogenase YP_933974.1 2727286 R 62928 CDS YP_933975.1 119898762 4609165 complement(2728444..2729406) 1 NC_008702.1 Ketopantoate reductase (KPA reductase) (KPR). Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid (By similarity). TIGRFAM: apbA_panE: 2-dehydropantoate 2-reductase Pfam: ApbA: Ketopantoate reductase PanE/ApbA; High confidence in function and specificity; 2-dehydropantoate 2-reductase 2729406 panE1 4609165 panE1 Azoarcus sp. BH72 2-dehydropantoate 2-reductase YP_933975.1 2728444 R 62928 CDS YP_933976.1 119898763 4609495 complement(2729490..2730527) 1 NC_008702.1 TRAP-dicarboxylate transporter. Binds c4-dicarboxylates; part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. 22% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide: present. TMHelix:1; High confidence in function and specificity; putative C4-dicarboxylate-binding periplasmic protein 2730527 dctP4 4609495 dctP4 Azoarcus sp. BH72 putative C4-dicarboxylate-binding periplasmic protein YP_933976.1 2729490 R 62928 CDS YP_933977.1 119898764 4608605 complement(2730630..2732087) 1 NC_008702.1 Probable benzaldehyde dehydrogenase [NAD+] (EC 1.2.1.28). Homology to xylC of P. putida of 53% (sprot|XYLC_PSEPU). CATALYTIC ACTIVITY: Benzaldehyde + NAD(+) + H(2)O = benzoate + NADH. Interpro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs proA: gamma-glutamyl phosphate reductas; Family membership; benzaldehyde dehydrogenase 2732087 xylC 4608605 xylC Azoarcus sp. BH72 benzaldehyde dehydrogenase YP_933977.1 2730630 R 62928 CDS YP_933978.1 119898765 4610132 complement(2732140..2733846) 1 NC_008702.1 catalyzes the formation of 2-acetolactate from pyruvate; also known as acetolactate synthase large subunit; acetolactate synthase catalytic subunit 2733846 alsS 4610132 alsS Azoarcus sp. BH72 acetolactate synthase catalytic subunit YP_933978.1 2732140 R 62928 CDS YP_933979.1 119898766 4608307 complement(2733998..2734894) 1 NC_008702.1 Transcriptional regulator,; High confidence in function and specificity; LysR family transcriptional regulator 2734894 dntR 4608307 dntR Azoarcus sp. BH72 LysR family transcriptional regulator YP_933979.1 2733998 R 62928 CDS YP_933980.1 119898767 4608642 2735125..2736588 1 NC_008702.1 Putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein. Homology to rdxA of R. sphaeroides of 37% (SWISSPROT:RDXA_RHOSH) Involved in a membrane generated redox signal. Pfam: 4Fe-4S binding domain Probable 5 TMHs no signal peptide; Family membership; putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein 2736588 rdxA 4608642 rdxA Azoarcus sp. BH72 putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein YP_933980.1 2735125 D 62928 CDS YP_933981.1 119898768 4609700 complement(2737170..2737286) 1 NC_008702.1 Putative hypothetical protein. Has extremely weak homologs in the the database. No signaficant known doamins,motifs or signal peptide present.; hypothetical protein 2737286 4609700 azo2477 Azoarcus sp. BH72 hypothetical protein YP_933981.1 2737170 R 62928 CDS YP_933982.1 119898769 4607437 2737435..2738619 1 NC_008702.1 Permease,member of the Major Facilitator Superfamiliy (MFS)transporters. MFS are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients. 34% MFS.IPR005829; Sug_transporter.IPR010916;TONB_Box_N. TMHelix:9.; Function unclear; putative MFS transporter permease 2738619 4607437 azo2478 Azoarcus sp. BH72 putative MFS transporter permease YP_933982.1 2737435 D 62928 CDS YP_933983.1 119898770 4607438 complement(2738628..2739335) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to VV12384 of Vibrio vulnificus of 60% (trembl|Q8DA26). No domains predicted. TMHMM2 reporting 1 TMH present. No signal peptide present.; Conserved hypothetical protein; hypothetical protein 2739335 4607438 azo2479 Azoarcus sp. BH72 hypothetical protein YP_933983.1 2738628 R 62928 CDS YP_933984.1 119898771 4607439 complement(2739453..2739998) 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 2739998 4607439 azo2480 Azoarcus sp. BH72 TetR family transcriptional regulator YP_933984.1 2739453 R 62928 CDS YP_933985.1 119898772 4607440 2740029..2740652 1 NC_008702.1 Conserved hypothetical membrane protein,; Conserved hypothetical protein; hypothetical protein 2740652 4607440 azo2481 Azoarcus sp. BH72 hypothetical protein YP_933985.1 2740029 D 62928 CDS YP_933986.1 119898773 4607441 complement(2740640..2745688) 1 NC_008702.1 Gene function unknown, no significant homology to proteins of known function. Typical domains for hybrid sensor and regulator are found. InterPro: IPR003594 ATPbind_ATPase. IPR004358 Bact_sens_pr_C. IPR005467 His_kinase. IPR003661 His_kinA_N. IPR001789 Response_reg. IPR008207 Hpt. IPR000014 PAS_domain. IPR001610 PAC. Pfam: PF00072 Response_reg. PF02518 HATPase_c. PF00512 HisKA. PF00989 PAS. PF00785 PAC. SMART: SM00387 HATPase_c. SM00388 HisKA. SM00448 REC. SM00091 PAS SM00086 PAC. TIGRFAM: TIGR00229 PAS domain S-box. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Conserved hypothetical protein; putative hybrid sensor and regulator 2745688 4607441 azo2482 Azoarcus sp. BH72 putative hybrid sensor and regulator YP_933986.1 2740640 R 62928 CDS YP_933987.1 119898774 4607442 complement(2745706..2746377) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Magn03007875 of Magnetospirillum magnetotacticum of 31% (gi|46202369|ref|ZP_00208493.1|(NBCI ENTREZ)). No domains predicted. Signal Peptide is reported to be Present, by Signal P program. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 2746377 4607442 azo2483 Azoarcus sp. BH72 hypothetical protein YP_933987.1 2745706 R 62928 CDS YP_933988.1 119898775 4607443 complement(2746361..2747515) 1 NC_008702.1 Putative response regulator,; Family membership; putative response regulator 2747515 4607443 azo2484 Azoarcus sp. BH72 putative response regulator YP_933988.1 2746361 R 62928 CDS YP_933989.1 119898776 4607444 complement(2747681..2747938) 1 NC_008702.1 Conserved hypothetical protein. Homology to eca2717 of E. carotovora of 35% (tremble:Q6D3M7). no domains predicted. no signal peptide. no TMHs; hypothetical protein 2747938 4607444 azo2485 Azoarcus sp. BH72 hypothetical protein YP_933989.1 2747681 R 62928 CDS YP_933990.1 119898777 4607445 complement(2747955..2750558) 1 NC_008702.1 Phosphoenolpyruvate synthase(Pyruvatewater dikinase)(PEP synthase). Essential step in gluconeogenesis when pyruvate and lactate are used as a carbon source.Catalytic activity:atp + pyruvate + h(2)o = amp + phosphoenolpyruvate + phosphate. 25% PEP_mobile.IPR006319; PEP_synth.IPR000121;PEP_utilizers.IPR002192; PPDK_N_term. Pfam:PF00391; PEP-utilizers; 1.PF02896; PEP-utilizers_C; 1. PF01326; PPDK_N; 1. TIGRFAMs:TIGR01418; PEP_synth; 1.; High confidence in function and specificity; putative phosphoenolpyruvate synthase 2750558 ppsA2 4607445 ppsA2 Azoarcus sp. BH72 putative phosphoenolpyruvate synthase YP_933990.1 2747955 R 62928 CDS YP_933991.1 119898778 4609616 complement(2750555..2751166) 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 2751166 4609616 azo2487 Azoarcus sp. BH72 TetR family transcriptional regulator YP_933991.1 2750555 R 62928 CDS YP_933992.1 119898779 4607446 complement(2751342..2751662) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2751662 4607446 azo2488 Azoarcus sp. BH72 hypothetical protein YP_933992.1 2751342 R 62928 CDS YP_933993.1 119898780 4607447 complement(2751828..2753672) 1 NC_008702.1 Integral membrane proteins that mediate the intake of a wide variety of molecules with the concomitant uptake of sodium ions(sodium symporters). Hypothetical E.coli protein yfbS. Similar to TrkA. The NAD+ -binding protein TrkA is a component of a low-affinity K+ uptake system in Escherichia coli. This protein has the TrkA-C and TrkA-N domains. dass: anion transporter; High confidence in function and specificity; putative transport protein 2753672 4607447 azo2489 Azoarcus sp. BH72 putative transport protein YP_933993.1 2751828 R 62928 CDS YP_933994.1 119898781 4607448 complement(2753758..2754147) 1 NC_008702.1 Putative Thioredoxin. Homology to txn (THIO_ICTPU) of I. punctatus of 36%. Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide. InterPro: Thioredoxin (IPR0006662) Tigrfam: dsbE: periplasmic protein thiol:disulfie Oxidoreductase, DSB subfamily Pfam: Thioredoxin no signal peptide no TMHs; Family membership; thioredoxin-disulfide reductase 2754147 txn 4607448 txn Azoarcus sp. BH72 thioredoxin-disulfide reductase YP_933994.1 2753758 R 62928 CDS YP_933995.1 119898782 4610052 complement(2754465..2755391) 1 NC_008702.1 Probable electron transfer flavoprotein, alpha subunit. Homology to etfA of B. japonicum of 65% (sprot|ETFA_BRAJA) The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein alpha-subunit (IPR001308) Pfam: Electron transfer flavoprotein alpha subunit no signal peptide no TMHs; High confidence in function and specificity; electron transfer flavoprotein subunit alpha 2755391 etfA3 4610052 etfA3 Azoarcus sp. BH72 electron transfer flavoprotein subunit alpha YP_933995.1 2754465 R 62928 CDS YP_933996.1 119898783 4608680 complement(2755391..2756140) 1 NC_008702.1 Electron transfer flavoprotein, beta subunit. Homology to etfB of B. japonicum of 71% (sprot|ETFB_BRAJA). The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein beta-subunit (IPR000049) Pfam: Electron transfer flavoprotein beta subunit no signal peptide no TMHS; High confidence in function and specificity; electron transfer flavoprotein subunit beta 2756140 etfB3 4608680 etfB3 Azoarcus sp. BH72 electron transfer flavoprotein subunit beta YP_933996.1 2755391 R 62928 CDS YP_933997.1 119898784 4608684 complement(2756182..2756715) 1 NC_008702.1 Entry name:- TREMBL:Q8XXS1 InterPro IPR002539; MaoC_dehydratas. Pfam PF01575; MaoC_dehydratas; 1. Identities = 74/154 (48%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; hypothetical protein 2756715 4608684 azo2493 Azoarcus sp. BH72 hypothetical protein YP_933997.1 2756182 R 62928 CDS YP_933998.1 119898785 4607449 complement(2756715..2758502) 1 NC_008702.1 The aidB gene encodes a protein of ca. 60 kDa that is homologous to several mammalian acyl coenzyme A dehydrogenases. Reaction:- acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor Entry name SWISSPROT:AIDB_ECOLI InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; 1. Identities = 85/289 (29%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; putative acyl-CoA dehydrogenase 2758502 aidB2 4607449 aidB2 Azoarcus sp. BH72 putative acyl-CoA dehydrogenase YP_933998.1 2756715 R 62928 CDS YP_933999.1 119898786 4608290 complement(2758926..2759345) 1 NC_008702.1 4-hydroxybenzoyl-CoA thioesterase (EC 3.1.2.23). This family contains a wide variety of enzymes,principally thioesterases. This family includes 4HBT which catalyses the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3 InterPro:IPR008272; 4HBcoA_thiost_AS. IPR006683; Thioestr_supf. Pfam:PF03061; 4HBT InterPro: 4-hydroxybenzoyl-CoA thioesterase family active site No signal peptide No transmembrane helices TIGR00051: conserved hypothetical protein; High confidence in function and specificity; thiesterase 2759345 fcbC1 4608290 fcbC1 Azoarcus sp. BH72 thiesterase YP_933999.1 2758926 R 62928 CDS YP_934000.1 119898787 4608735 complement(2759364..2760131) 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,; Specificity unclear; short-chain dehydrogenase 2760131 4608735 azo2496 Azoarcus sp. BH72 short-chain dehydrogenase YP_934000.1 2759364 R 62928 CDS YP_934001.1 119898788 4607450 complement(2760328..2761587) 1 NC_008702.1 Thiolytic cleavage of beta-ketoadipyl-CoA to succinate and acetyl-CoA (By similarity). Entry name PAAJ_ECOLI Primary accession number P77525 Identity: 150/410 (36%) InterPro IPR002155; Thiolase. Pfam PF02803; Thiolase_C; 1. Number of predicted TMHs: 0 Prediction: Non-secretory protein Signal peptide probability: 0.000; Family membership; putative beta-ketoadipyl CoA thiolase 2761587 paaJ2 4607450 paaJ2 Azoarcus sp. BH72 putative beta-ketoadipyl CoA thiolase YP_934001.1 2760328 R 62928 CDS YP_934002.1 119898789 4609482 complement(2761584..2762369) 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 2762369 paaG3 4609482 paaG3 Azoarcus sp. BH72 enoyl-CoA hydratase YP_934002.1 2761584 R 62928 CDS YP_934003.1 119898790 4609474 complement(2762390..2764258) 1 NC_008702.1 catalyzes the conversion of ferulic acid to feruloyl-CoA; feruloyl-CoA synthase 2764258 fadD2 4609474 fadD2 Azoarcus sp. BH72 feruloyl-CoA synthase YP_934003.1 2762390 R 62928 CDS YP_934004.1 119898791 4608723 complement(2764287..2765432) 1 NC_008702.1 In enteric bacteria such as E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar sprot|LIVK_ECOLI (21%) and to trembl|Q9RYP6 (50%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative leucine-specific binding protein 2765432 livK2 4608723 livK2 Azoarcus sp. BH72 putative leucine-specific binding protein YP_934004.1 2764287 R 62928 CDS YP_934005.1 119898792 4609154 complement(2765867..2766628) 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q8XRX4 (66%) and to trembl|Q89PQ7 (62%). Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 2766628 4609154 azo2501 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934005.1 2765867 R 62928 CDS YP_934006.1 119898793 4607451 complement(2766628..2768421) 1 NC_008702.1 Probable branched-chain amino acid ABC transporterATP binding protein,; Specificity unclear; branched-chain amino acid ABC transporter 2768421 4607451 azo2502 Azoarcus sp. BH72 branched-chain amino acid ABC transporter YP_934006.1 2766628 R 62928 CDS YP_934007.1 119898794 4607452 complement(2768487..2769524) 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q7W5E8 (62%), to sprot|BRAD_PSEAE (25%) and to sprot|LIVH_ECOLI (26%). Pfam (PF02653): Branched-chain amino acid transport system / permease component TMHMM reporting nine Tmhelix.; Specificity unclear; ABC transporter permease 2769524 4607452 azo2503 Azoarcus sp. BH72 ABC transporter permease YP_934007.1 2768487 R 62928 CDS YP_934008.1 119898795 4607453 2769967..2770881 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 2770881 4607453 azo2504 Azoarcus sp. BH72 TetR family transcriptional regulator YP_934008.1 2769967 D 62928 CDS YP_934009.1 119898796 4607454 2771064..2771702 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 2771702 4607454 azo2505 Azoarcus sp. BH72 TetR family transcriptional regulator YP_934009.1 2771064 D 62928 CDS YP_934010.1 119898797 4607455 2771799..2772377 1 NC_008702.1 Conserved hypothetical protein. The highly identitcal hits such as Hypothetical Proteins of TrEMBL;Q8XXB7,Q6N0V6(80% identity) do not share all the domains as this Protein. Has Carboxymuconolactone decarboxylase family;IPR003779, CMD:The catechol and protocatechuate branches of the 3-oxoadipate pathway,which areimportant for the bacterial degradation of aromatic compounds, converge at the common intermediate 3-oxoadipate enol-lactone. Carboxymuconolactone decarboxylase is involved in protocatechuate catabolism. In some bacteria a gene fusion event leads to expression of CMD with a hydrolase involved in the same pathway.; Specificity unclear; 4-carboxymuconolactone decarboxylase 2772377 cmd 4607455 cmd Azoarcus sp. BH72 4-carboxymuconolactone decarboxylase YP_934010.1 2771799 D 62928 CDS YP_934011.1 119898798 4608494 complement(2772486..2772884) 1 NC_008702.1 Hypothetical membrane protein. no homology of the entire protein to the data bank. no domains predicted. no signal peptide. 2 TMHS; hypothetical protein 2772884 4608494 azo2507 Azoarcus sp. BH72 hypothetical protein YP_934011.1 2772486 R 62928 CDS YP_934012.1 119898799 4607456 complement(2772945..2773253) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003856 of Dechloromonas aromatica of 41% (gi|46140330|ref|ZP_00203564.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 2773253 4607456 azo2508 Azoarcus sp. BH72 hypothetical protein YP_934012.1 2772945 R 62928 CDS YP_934013.1 119898800 4607457 complement(2773297..2775222) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv0086 of C. violaceum of 76% (trembl|Q7P1X6). no signal peptide. no TMHs. No domains reported.; hypothetical protein 2775222 4607457 azo2509 Azoarcus sp. BH72 hypothetical protein YP_934013.1 2773297 R 62928 CDS YP_934014.1 119898801 4607458 complement(2775497..2775781) 1 NC_008702.1 Bacterial ring hydroxylating dioxygenase,small subunit. Similar to AlcE: iron-sulfur-containing dioxygenases, probably involved in alcaligin biosynthesis pathway in Bordetella species. Putative iron-sulfure protein involved in aromatic compounds degradations. Rieske [2Fe-2S] domain present. Probable Choline monooxygenase chloroplast precursor (EC 1.14.15.7). nadp_idh_euk: isocitrate dehydrogenase; Function unclear; putative iron-sulfur protein 2775781 4607458 azo2510 Azoarcus sp. BH72 putative iron-sulfur protein YP_934014.1 2775497 R 62928 CDS YP_934015.1 119898802 4607459 complement(2776065..2778491) 1 NC_008702.1 Iron-regulated outer membrane protein. TonB-dependent receptor protein. Homolog to fpvA, a ferripyoverdine receptor precursor in P. aeruginosa. Similar to PupA protein of P. putida WCS,this protein is a receptor for the iron-bound form of pesudobactin, a compound structurally very; High confidence in function and specificity; putative TonB-dependent receptor 2778491 fpvA1 4607459 fpvA1 Azoarcus sp. BH72 putative TonB-dependent receptor YP_934015.1 2776065 R 62928 CDS YP_934016.1 119898803 4608827 complement(2778614..2779630) 1 NC_008702.1 Probable FecR-like transmembrane sensor involved in iron metabolism. FecR is involved in regulation of iron dicitrate transport. In the absence of citrate FecR inactivates FecI. FecR is probably a sensor that recognizes iron dicitrate in the periplasm.; High confidence in function and specificity; putative transmembrane sensor 2779630 4608827 azo2512 Azoarcus sp. BH72 putative transmembrane sensor YP_934016.1 2778614 R 62928 CDS YP_934017.1 119898804 4607460 complement(2779714..2780268) 1 NC_008702.1 Probable fecI-like RNA polymerase sigma factor. THE SIGMA FACTOR IS AN INITIATION FACTOR THAT PROMOTES ATTACHMENT OF THE RNA POLYMERASE TO SPECIFIC INITIATION SITES AND THEN IS RELEASED. THIS SIGMA FACTOR REGULATES THE FEC GENES FOR IRON DICITRATE TRANSPORT (PROBABLE). InterPro: Sigma factor ECF subfamily; Specificity unclear; RNA polymerase ECF-subfamily sigma factor 2780268 4607460 azo2513 Azoarcus sp. BH72 RNA polymerase ECF-subfamily sigma factor YP_934017.1 2779714 R 62928 CDS YP_934018.1 119898805 4607461 complement(2780947..2781264) 1 NC_008702.1 Naphthalene 12-dioxygenase system ferredoxin component. COMPONENT OF NAPHTHALENE DIOXYGENASE (NDO) MULTICOMPONENT ENZYME SYSTEM WHICH CATALYZES THE INCORPORATION OF BOTH ATOMS OF MOLECULAR OXYGEN INTO NAPHTHALENE TO FORM CIS- NAPHTHALENE DIHYDRODIOL. THIS SUBUNIT IS A 2FE-2S FERREDOXIN THAT TRANSFERS ELECTRONS TO IRON SULFUR PROTEIN COMPONENTS (ISP).; High confidence in function and specificity; putative ferredoxin 2781264 nagAb 4607461 nagAb Azoarcus sp. BH72 putative ferredoxin YP_934018.1 2780947 R 62928 CDS YP_934019.1 119898806 4609308 complement(2781315..2781818) 1 NC_008702.1 NagH: probable salicylate-5-hydroxylase small oxygenase component oxidoreductase. Involved in conversion of Naphthalene to gentisate. Aromatic compounds degradation.; Family membership; putative ring hydroxylating subunit beta 2781818 nagH 4609308 nagH Azoarcus sp. BH72 putative ring hydroxylating subunit beta YP_934019.1 2781315 R 62928 CDS YP_934020.1 119898807 4609310 complement(2781815..2783131) 1 NC_008702.1 nagG,RSc1090:putative salicylate-5-hydroxylase large oxygenase component oxidoreductase [EC:1.-.-.-]. Rieske-type iron-sulfur center homologous to the large subunits of dihydroxylating dioxygenases. Involved in conversion of Naphthalene to gentisate. Aromatic compounds degradation. Biphenyl dioxygenase alpha subunit (EC 1.14.12.18) (Biphenyl 23- dioxygenase).; High confidence in function and specificity; putative ring hydroxylating large subunit 2783131 nagG 4609310 nagG Azoarcus sp. BH72 putative ring hydroxylating large subunit YP_934020.1 2781815 R 62928 CDS YP_934021.1 119898808 4609309 complement(2783542..2784528) 1 NC_008702.1 Naphthalene 12-dioxygenase system ferredoxin--NAD(+) reductase component COMPONENT OF NAPHTHALENE DIOXYGENASE (NDO) MULTICOMPONENT ENZYME SYSTEM WHICH CATALYZES THE INCORPORATION OF BOTH ATOMS OF MOLECULAR OXYGEN INTO NAPHTHALENE TO FORM CIS- NAPHTHALENE DIHYDRODIOL. TRANSFERS ELECTRONS FROM FERREDOXIN (NDOA) TO NADH.; High confidence in function and specificity; naphthalene 1,2-dioxygenase/ferredoxin--NAD(+) reductase 2784528 nagAa 4609309 nagAa Azoarcus sp. BH72 naphthalene 1,2-dioxygenase/ferredoxin--NAD(+) reductase YP_934021.1 2783542 R 62928 CDS YP_934022.1 119898809 4609307 2784640..2785581 1 NC_008702.1 LysR-type transcriptional regulator NahR Regulates the expression of the naphthalene (nahA-F) and salicylate (nahG-M) metabolism genes. Similar to SWISSPROT: sprot|NAHR_PSEPU (61% Pseudomonas putida, nahR) Pfam: PF00126 Bacterial regulatory helix-turn-helix protein,lysR family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; LysR family transcriptional regulator 2785581 nahR2 4609307 nahR2 Azoarcus sp. BH72 LysR family transcriptional regulator YP_934022.1 2784640 D 62928 CDS YP_934023.1 119898810 4609325 complement(2785687..2787012) 1 NC_008702.1 TRAP-type C4-dicarboxylate transport system, large permease component,DctM. The dct locus encodes a high-affinity transport system for the C4-dicarboxylates malate,succinate, and fumarate. 25% DctM.IPR000252; DedA.IPR000524; HTH_GntR.IPR004681; TRAP_transptDctM. Pfam:PF06808; DctM; 1.PF00597; DedA; 1. TIGRFAMs:TIGR00786; dctM; 1. TMHelix: 11.; Specificity unclear; TRAP-type C4 dicarboxylate transport system large permease 2787012 dctM5 4609325 dctM5 Azoarcus sp. BH72 TRAP-type C4 dicarboxylate transport system large permease YP_934023.1 2785687 R 62928 CDS YP_934024.1 119898811 4608601 complement(2787009..2787533) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PM1526 of Pasteurella multocida of 32% (trembl|Q9CKT0(SRS)). Pfam: PF04290;IPR007387;Tripartite ATP-independent periplasmic transporters, DctQ component; The function of the members of this family is unknown, but DctQ homologues are invariably found in the tripartite ATP-independent periplasmic transporters. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2787533 4608601 azo2520 Azoarcus sp. BH72 hypothetical protein YP_934024.1 2787009 R 62928 CDS YP_934025.1 119898812 4607462 complement(2787622..2788647) 1 NC_008702.1 TRAP-dicarboxylate transporter. Binds c4-dicarboxylates; part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. 23% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide: present.; Specificity unclear; putative C4-dicarboxylate-binding periplasmic protein 2788647 dctP5 4607462 dctP5 Azoarcus sp. BH72 putative C4-dicarboxylate-binding periplasmic protein YP_934025.1 2787622 R 62928 CDS YP_934026.1 119898813 4608606 complement(2788910..2789752) 1 NC_008702.1 extradiol catechol dioxygenase that catalyzes the oxidative cleavage of substituted catechols; part of the bacterial aromatic compound degradation pathway; protocatechuate 4,5-dioxygenase subunit beta 2789752 ligB1 4608606 ligB1 Azoarcus sp. BH72 protocatechuate 4,5-dioxygenase subunit beta YP_934026.1 2788910 R 62928 CDS YP_934027.1 119898814 4609141 complement(2789756..2790106) 1 NC_008702.1 extradiol catechol dioxygenase that catalyzes the oxidative cleavage of substituted catechols; part of the bacterial aromatic compound degradation pathway; protocatechuate 4,5-dioxygenase subunit alpha 2790106 ligA1 4609141 ligA1 Azoarcus sp. BH72 protocatechuate 4,5-dioxygenase subunit alpha YP_934027.1 2789756 R 62928 CDS YP_934028.1 119898815 4609139 2790194..2791156 1 NC_008702.1 Transcriptional regulator, LysR family,; Specificity unclear; LysR family transcriptional regulator 2791156 pnbR 4609139 pnbR Azoarcus sp. BH72 LysR family transcriptional regulator YP_934028.1 2790194 D 62928 CDS YP_934029.1 119898816 4609588 2791543..2793033 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. Pfam: Endo/excinuclease amino terminal domain. no signal peptide. no TMHs. HTH predicted; hypothetical protein 2793033 4609588 azo2525 Azoarcus sp. BH72 hypothetical protein YP_934029.1 2791543 D 62928 CDS YP_934030.1 119898817 4607463 complement(2793435..2794322) 1 NC_008702.1 Transcriptional regulator, AraC family,; High confidence in function and specificity; AraC family transcriptional regulator 2794322 pobR2 4607463 pobR2 Azoarcus sp. BH72 AraC family transcriptional regulator YP_934030.1 2793435 R 62928 CDS YP_934031.1 119898818 4609595 2794548..2795711 1 NC_008702.1 Cytochrome P450s are involved in the oxidative degradation of various compounds. Particularly well known for their role in the degradation of environmental toxins and mutagens. Similar to SWISSPROT:P77902 (30% identity); TREMBL:Q59910 (32% identity); SWISSPROT:O34374 (28% identity). Pfam (PF00067): Cytochrome P450.; Family membership; cytochrome P450 family protein 2795711 4609595 azo2527 Azoarcus sp. BH72 cytochrome P450 family protein YP_934031.1 2794548 D 62928 CDS YP_934032.1 119898819 4607464 2795797..2796120 1 NC_008702.1 Probable ferredoxin. Homology to fdxP of C. crescentus of 51% (sprot|FER2_CAUCR). Ferredons are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. InterPro: Ferredoxin (IPR001041),Adrenodoxin (IPR001055) Pfam: 2Fe-2S iron-sulfur cluster binding domain no signal peptide no TMHs; High confidence in function and specificity; ferrodoxin 2796120 fdxP 4607464 fdxP Azoarcus sp. BH72 ferrodoxin YP_934032.1 2795797 D 62928 CDS YP_934033.1 119898820 4608752 2796181..2797398 1 NC_008702.1 Probable putidaredoxin reductase (EC 1.18.1.-). Homology to camA of P. putida of 48% (sprot|CAMA_PSEPU). The oxidation of camphor by cytochrome p450-cam requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from nadh to p450 for oxygen activation. Interpro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IRP001327), NAD binding site (IPR000205) Pfam: Pyridine nucleotide-disulphide oxidoreductase no signal peptide no TMHs; Family membership; putidaredoxin reductase 2797398 camA 4608752 camA Azoarcus sp. BH72 putidaredoxin reductase YP_934033.1 2796181 D 62928 CDS YP_934034.1 119898821 4608412 2797558..2798433 1 NC_008702.1 Hypothetical protein yqgX. 65% identity, 75% similarity to conserved hypothetical protein (TREMBL:Q7P219) InterPro; TREMBL:Q7WQV2; Metallo-beta-lactamase superfamily. Pfam: Aminotransferase class III; helper component proteinase TREMBL:Q9DCMO-RIKEN cDNA; TREMBL:Q9A911 - GlyoxlaseII superfamily protein. TIGR00162: conserved hypothetical protein. Signal peptide-nonsecretory protein; Transmembrane helices present.; Function unclear; hypothetical protein 2798433 4608412 azo2530 Azoarcus sp. BH72 hypothetical protein YP_934034.1 2797558 D 62928 CDS YP_934035.1 119898822 4607465 complement(2798436..2799140) 1 NC_008702.1 Catalyzes the transfer of the ammonia group from glutamine to a new carbon-nitrogen group; glutamine amidotransferase 2799140 4607465 azo2531 Azoarcus sp. BH72 glutamine amidotransferase YP_934035.1 2798436 R 62928 CDS YP_934036.1 119898823 4607466 2799319..2799741 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2799741 4607466 azo2532 Azoarcus sp. BH72 hypothetical protein YP_934036.1 2799319 D 62928 CDS YP_934037.1 119898824 4607467 2799878..2801560 1 NC_008702.1 Flagellin (Phase-1-D flagellin). Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. InterPro: Flagellin N-terminus,Flagellin C-terminus Pfam: Bacterial flagellin N-terminus,bacterial flagellin C-terminus no signal peptide no TMH; Family membership; putative flagellin 2801560 fliC1 4607467 fliC1 Azoarcus sp. BH72 putative flagellin YP_934037.1 2799878 D 62928 CDS YP_934038.1 119898825 4608795 complement(2801557..2802510) 1 NC_008702.1 Putative malonate transporter. TREMBL:Q7ML40: 38% identity, 55% similarity SPROT:P56949: 27% identity, 44% similarity subcellular location:integral membrane protein (potential). similarity:belongs to the auxin efflux carrier (tc 2.a.69) family. InterPro:IPR004776; Auxin_eff. Pfam: PF03547; Auxin_eff; GntP_permease;GntP family permease (Pfam) 2a69: Auxin Efflux Carrier Signal P predicted signal peptide and TMHMM predicted 10 transmembrane helices; Specificity unclear; putative malonate transporter 2802510 mdcF2 4608795 mdcF2 Azoarcus sp. BH72 putative malonate transporter YP_934038.1 2801557 R 62928 CDS YP_934039.1 119898826 4609202 complement(2802611..2803606) 1 NC_008702.1 Probable aldo-keto reductase. Homology to yakC of S.pombe of 43% (sprot|YAKC_SCHPO). Catalyze the reduction of 2-nitorbenzaldehyde, pyridine-2-aldehyde and 2-phthaladehyde. Pfam: Aldo/Keto reductase signal peptide no TMHs; Family membership; aldo-keto reductase 2803606 yakC 4609202 yakC Azoarcus sp. BH72 aldo-keto reductase YP_934039.1 2802611 R 62928 CDS YP_934040.1 119898827 4610142 complement(2804153..2805112) 1 NC_008702.1 Hypothetical oxidoreductase yrbE (EC 1.-.-.-). TREMBL:Q93PS4:90% identity; 93% similarity. This group of enzymes utilise NADP or NAD, and is known as the GFO/IDH/MOCA family in Swiss-Prot. GFO is a glucose--fructose oxidoreductase, which converts D-glucose and D-fructose into D-gluconolactone and D-glucitol in the sorbitol-gluconate pathway. MOCA is a rhizopine catabolism protein which may catalyze the NADH-dependent dehydrogenase reaction involved in rhizopine catabolism. Other proteins belonging to this family include Gal80, a negative regulator for the expression of lactose and galactose metabolic genes; and several hypothetical proteins from yeast, Escherichia coli and Bacillus subtilis. InterPro:IPR000683; GFO_IDH_MocA. IPR004104; GFO_IDH_MocA_C. Pfam: PF01408; GFO_IDH_MocA; 1. PF02894; GFO_IDH_MocA_C; presence of DUF206 Probably nonsecretory protein (SignalP predicted) Number of predicted TMHs: 0 (TMHMM predicted) InterPro: Oxidoreductase N-terminal ubiX: phenylacrylic acid decarboxylase 3; High confidence in function and specificity; oxidoreductase 2805112 ligC 4610142 ligC Azoarcus sp. BH72 oxidoreductase YP_934040.1 2804153 R 62928 CDS YP_934041.1 119898828 4609143 complement(2805209..2806063) 1 NC_008702.1 extradiol catechol dioxygenase that catalyzes the oxidative cleavage of substituted catechols; part of the bacterial aromatic compound degradation pathway; protocatechuate 4,5-dioxygenase subunit beta 2806063 ligB2 4609143 ligB2 Azoarcus sp. BH72 protocatechuate 4,5-dioxygenase subunit beta YP_934041.1 2805209 R 62928 CDS YP_934042.1 119898829 4609142 complement(2806066..2806506) 1 NC_008702.1 extradiol catechol dioxygenase that catalyzes the oxidative cleavage of substituted catechols; part of the bacterial aromatic compound degradation pathway; protocatechuate 4,5-dioxygenase subunit alpha 2806506 ligA2 4609142 ligA2 Azoarcus sp. BH72 protocatechuate 4,5-dioxygenase subunit alpha YP_934042.1 2806066 R 62928 CDS YP_934043.1 119898830 4609140 complement(2807012..2807926) 1 NC_008702.1 Probable 2-pyrone-4,6-dicarboxylate hydrolase. Homology to ligI of S. paucimobilis of 55% (tremble:O87170) Pfam: Amidohydrolase no signal peptide no TMHs; High confidence in function and specificity; 2-pyrone-4,6-dicarboxylate hydrolase 2807926 ligI 4609140 ligI Azoarcus sp. BH72 2-pyrone-4,6-dicarboxylate hydrolase YP_934043.1 2807012 R 62928 CDS YP_934044.1 119898831 4609144 complement(2807967..2808902) 1 NC_008702.1 Activity:- 3-hydroxy-2-methylpropanoate + NAD = 2-methyl-3-oxopropanoate + NADH2 Entry name SWISSPROT:MMSB_PSEAE InterPro IPR002204; 3hydroxisobut_dh. IPR006115; 6PGD_NAD. Pfam PF03446; NAD_binding_2; 1. Identities = 48/218 (22%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; putative 3-hydroxyisobutyrate dehydrogenase 2808902 mmsB 4609144 mmsB Azoarcus sp. BH72 putative 3-hydroxyisobutyrate dehydrogenase YP_934044.1 2807967 R 62928 CDS YP_934045.1 119898832 4609231 complement(2808905..2809594) 1 NC_008702.1 Possible acyl transferase.; Family membership; hypothetical protein 2809594 4609231 azo2541 Azoarcus sp. BH72 hypothetical protein YP_934045.1 2808905 R 62928 CDS YP_934046.1 119898833 4607468 complement(2809613..2810638) 1 NC_008702.1 4-oxalomesaconate hydratase These proteins are related to the metal-dependent hydrolase superfamily tremblnew|CAE30137: 86% identity, 93% similarity InterPro:IPR006992; Amidohydro_2. Pfam:PF04909; Amidohydro_2 Absence of traansmembrane helices (TMHMM predicted); High confidence in function and specificity; 4-oxalomesaconate hydratase 2810638 fldW 4607468 fldW Azoarcus sp. BH72 4-oxalomesaconate hydratase YP_934046.1 2809613 R 62928 CDS YP_934047.1 119898834 4608772 complement(2810683..2812047) 1 NC_008702.1 PcaK:4-hydroxybenzoate transporter. TRANSPORTER FOR 4-HYDROXYBENZOATE ALSO REQUIRED FOR CHEMOTAXIS TO AROMATIC ACIDS. Membership of the major facilitator superfamily of transport proteins. 2A0115: benzoate transport; High confidence in function and specificity; putative 4-hydroxybenzoate transporter protein 2812047 pcaK 4608772 pcaK Azoarcus sp. BH72 putative 4-hydroxybenzoate transporter protein YP_934047.1 2810683 R 62928 CDS YP_934048.1 119898835 4609505 complement(2812166..2813248) 1 NC_008702.1 Hypothetical protein FldA, 78% identity (86% similarity) to TrEMBL;Q6N0R5,Q8PF30(47% identity). 45% identity to ybhH of SwissProt;P75762(E.coli) Has PF04303,Protein of unknown function (DUF453);IPR007400; FldA (Q9L3A0) is thought to be involved in the degradation of the polyaromatic hydrocarbon fluorene by Sphingomonas sp. LB126. Has PF01678, Diaminopimelate epimerase;IPR001653 DAP_epimerase: Diaminopimelate epimerase contains two domains of the same alpha/beta fold, both contained in this family.Diaminopimelate epimerase (EC:5.1.1.7) catalyzes the isomeriazation of L,L- to D,L-meso-diaminopimelate in the biosynthetic pathway leading from aspartate to lysine. This enzyme is a protein of about 30 kDa. No signal peptide or TMH present.; Family membership; hypothetical protein 2813248 fldA 4609505 fldA Azoarcus sp. BH72 hypothetical protein YP_934048.1 2812166 R 62928 CDS YP_934049.1 119898836 4608771 complement(2813370..2814611) 1 NC_008702.1 Bicyclomycin resistance protein homolog. Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase (By similarity). InterPro: General substrate transporters efflux_Bcr_CflA: drug resistance tran Also show similarity to bssH of bssDCABEFGH operon-genes involved in anaerobic degradation of toulene in denitryfing bacterium, Azoarcus strain EbN1; High confidence in function and specificity; putative bicyclomycin resistance protein 2814611 bcr1 4608771 bcr1 Azoarcus sp. BH72 putative bicyclomycin resistance protein YP_934049.1 2813370 R 62928 CDS YP_934050.1 119898837 4608382 2814613..2815779 1 NC_008702.1 Nitrogen assimilation regulatory protein nac (Nitrogen assimilation control protein). TRANSCRIPTIONAL ACTIVATOR FOR THE HUT PUT AND URE OPERONS AND REPRESSOR FOR THE GDH AND GLTB OPERONS IN RESPONSE TO NITROGEN LIMITATION. NEGATIVE REGULATOR OF ITS OWN EXPRESSION. Similar to SWISSPROT: sprot|NAC_KLEAE (30% Klebsiella aerogenes, nitrogen assimilation regulatory protein nac (nitrogen assimilation control protein)) Pfam: PF00126 Bacterial regulatory helix-turn-helix protein, lysR family. HTH reporting nucleic acid binding motif.; Specificity unclear; putative nitrogen assimilation regulatory protein 2815779 nac 4608382 nac Azoarcus sp. BH72 putative nitrogen assimilation regulatory protein YP_934050.1 2814613 D 62928 CDS YP_934051.1 119898838 4609301 2815984..2817636 1 NC_008702.1 Probable methyl-accepting chemotaxis protein,; Specificity unclear; methyl-accepting chemotaxis protein 2817636 4609301 azo2547 Azoarcus sp. BH72 methyl-accepting chemotaxis protein YP_934051.1 2815984 D 62928 CDS YP_934052.1 119898839 4607469 complement(2817706..2818587) 1 NC_008702.1 Getrichum candidum Dec1,Dye-decolorizing peroxidase,DyP, that lacks a typical heme-binding region. Responsible for the decoloration of dyes. DyP degraded phenolic compounds, such as 2,6-dimethoxyphenol and guaiacol. Hypothetical protein yfeX. ccoO: cytochrome c oxidase cbb3-type su; High confidence in function and specificity; putative iron-dependent peroxidase 2818587 dyp 4607469 dyp Azoarcus sp. BH72 putative iron-dependent peroxidase YP_934052.1 2817706 R 62928 CDS YP_934053.1 119898840 4608660 complement(2818660..2819562) 1 NC_008702.1 tRNA nucleotidyltransferase (EC 2.7.7.25) (tRNA adenylyltransferase) (tRNA CCA-pyrophosphorylase) (CCA-adding enzyme). This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.; Family membership; tRNA nucleotidyltransferase 2819562 cca2 4608660 cca2 Azoarcus sp. BH72 tRNA nucleotidyltransferase YP_934053.1 2818660 R 62928 CDS YP_934054.1 119898841 4608442 2819724..2820674 1 NC_008702.1 Putative malonate transporter. TREMBL:Q8ZCU0: 38% identity, 55% similarity SPROT:P56949:23% identity, 41% similarity InterPro:IPR004776; Auxin_eff. Pfam:PF03547; Auxin_ef Signal P predicted nonsecretory protein and TMHMM predicted 9 transmembrane helices. 2a69: Auxin Efflux Carrier; Specificity unclear; putative malonate transporter 2820674 mdcF3 4608442 mdcF3 Azoarcus sp. BH72 putative malonate transporter YP_934054.1 2819724 D 62928 CDS YP_934055.1 119898842 4609203 2821091..2821981 1 NC_008702.1 Conserved hypothetical membrane protein. Homology with PA3897 of P. aeruginosa of 30%. InterPro: Integral membrane protein DUF6. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transport. Pfam: Integral membrane protein DUF6. probable 10 TMHs. no signal peptide; Conserved hypothetical protein; hypothetical protein 2821981 4609203 azo2551 Azoarcus sp. BH72 hypothetical protein YP_934055.1 2821091 D 62928 CDS YP_934056.1 119898843 4607470 2822144..2823451 1 NC_008702.1 Tfp pilus retraction protein PilU, probable involved in the twitching motility mechanism,; High confidence in function and specificity; twitching motility protein 2823451 pilU1 4607470 pilU1 Azoarcus sp. BH72 twitching motility protein YP_934056.1 2822144 D 62928 CDS YP_934057.1 119898844 4609574 2823562..2824104 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. TMH in signal peptide; hypothetical protein 2824104 4609574 azo2553 Azoarcus sp. BH72 hypothetical protein YP_934057.1 2823562 D 62928 CDS YP_934058.1 119898845 4607471 2824434..2825978 1 NC_008702.1 Putative outer membrane efflux protein. Homology to nodT from Rhizobium leguminosarum of 20% over 432 aa. The OEP family (Outer membrane efflux protein)allow export of a variety of substrates in Gram negative bacteria. Most members of NodT family are likely to export primarily small molecules rather than proteins. InterPro: Outer membrane efflux protein. Pfam: Outer membrane efflux protein Signal peptide no TMHs; Family membership; putative outer membrane efflux protein 2825978 nodT 4607471 nodT Azoarcus sp. BH72 putative outer membrane efflux protein YP_934058.1 2824434 D 62928 CDS YP_934059.1 119898846 4609392 2825984..2826748 1 NC_008702.1 Specificity unclear; HlyD family secretion protein 2826748 4609392 azo2555 Azoarcus sp. BH72 HlyD family secretion protein YP_934059.1 2825984 D 62928 CDS YP_934060.1 119898847 4607472 2826820..2832576 1 NC_008702.1 No Good Homology with any hits in the DB. Conserved Hypothetical Protein,34% similarity to TrEMBL;Q8E9W3. Has PF03160:IPR003644:Calx_beta;Na-Ca exchanger/integrin-beta4:This domain has been found in Na-Ca exchangers and integrin subunit beta4, as well as some cyanobacterial proteins. Has 3 CA(Cadherin repeats)domains;SMART:SM00112;IPR002126:Cadherins are glycoproteins involved in Ca2+-mediated cell-cell adhesion. Cadherin domains occur as repeats in the extracellular regions which are thought to mediate cell-cell contact when bound to calcium. Has 1 CADG,Dystroglycan-type cadherin-like domains(SMART;SM00736).Cadherin-homologous domains present in metazoan dystroglycans and alpha/epsilon sarcoglycans,yeast Axl2p and in a very large protein from magnetotactic bacteria. Likely to bind calcium ions.; Family membership; hypothetical protein 2832576 4607472 azo2556 Azoarcus sp. BH72 hypothetical protein YP_934060.1 2826820 D 62928 CDS YP_934061.1 119898848 4607473 2832576..2833937 1 NC_008702.1 Similar to MacA.; Family membership; HlyD family secretion protein 2833937 4607473 azo2557 Azoarcus sp. BH72 HlyD family secretion protein YP_934061.1 2832576 D 62928 CDS YP_934062.1 119898849 4607474 2833940..2836051 1 NC_008702.1 Family membership; HlyD domain-containing protein 2836051 4607474 azo2558 Azoarcus sp. BH72 HlyD domain-containing protein YP_934062.1 2833940 D 62928 CDS YP_934063.1 119898850 4607475 2836162..2836581 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Mflag03001539 of Methylobacillus flagellatus of 38% (gi|45521149|ref|ZP_00172671.1|(NBCI ENTREZ)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2836581 4607475 azo2559 Azoarcus sp. BH72 hypothetical protein YP_934063.1 2836162 D 62928 CDS YP_934064.1 119898851 4607476 2836767..2837564 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc1124 of R. solanacearum of 59% (trembl|Q8Y0C0). no signal peptide. no TMHs. no domains reported.; hypothetical protein 2837564 4607476 azo2560 Azoarcus sp. BH72 hypothetical protein YP_934064.1 2836767 D 62928 CDS YP_934065.1 119898852 4607477 complement(2837663..2839060) 1 NC_008702.1 Conserved hypothetical protein. Homology to dr0392 of D. radiodurans of 33% (trembl|Q9RXC3). Pfam: Collagen triple helix repeat (20 copies). Members of this family belong to the collagen superfamily. Collagens are generally extracellular structural proteins involved in formation of connective tissue structure. Smart: Bacterial OsmY and nodulation domain (BON). The BON domain is typically ~60 residues long and has an alpha/beta predicted fold. There is a conserved glycine residue and several hydrophobic regions. This pattern of conservation is more suggestive of a binding or structural function rather than a catalytic function. Most proteobacteria seem to possess one or two BON-containing proteins, typically of the OsmY-type proteins. no signal peptide. no TMHs; hypothetical protein 2839060 4607477 azo2561 Azoarcus sp. BH72 hypothetical protein YP_934065.1 2837663 R 62928 CDS YP_934066.1 119898853 4607478 2839343..2840350 1 NC_008702.1 Cytochrome c peroxidase (EC 1.11.1.5), an electron-transfer proteins having one or several haem c groups,bound to the protein by one or, more generally, two thioether bonds involving sulphydryl groups of cysteine residues. The fifth haem iron ligand is always provided by a histidine residue. CytC possess a wide range of properties and function in a large number of different redox processes. Signal peptide.; High confidence in function and specificity; putative cytochrome c peroxidase 2840350 ccp 4607478 ccp Azoarcus sp. BH72 putative cytochrome c peroxidase YP_934066.1 2839343 D 62928 CDS YP_934067.1 119898854 4608459 2840530..2840916 1 NC_008702.1 Conserved hypothetical secreted secreted protein. Homology to bb1301 of B. bronchiseptica of 59% (trembl|Q7WMT9). Pfam: Lipoprotein_15. This family occurs as tandem repeats in a set of lipoproteins. The alignment contains a Y-X4-D motif. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2840916 4608459 azo2563 Azoarcus sp. BH72 hypothetical protein YP_934067.1 2840530 D 62928 CDS YP_934068.1 119898855 4607479 2840924..2841424 1 NC_008702.1 Putative RNA polymerase sigma factor,57% Identity to TrEMBL;Q7VV01, 49% Identity to TrEMBL;Q8XV48,53% to Q63LJ3. Has PF04542, Sigma-70 region 2; IPR007627,Sigma70_r2; Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA polymerase binding determinant. The core binding helix, interacts with the clamp domain of the largest polymerase subunit,beta prime. The aromatic residues of the recognition helix, found at the C-terminus of this domain are though to mediate strand separation, thereby allowing transcription initiation. Has PF04545, Sigma-70, region 4;IPR007630, Sigma70_r4; Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix families. Therefore there are many false negatives.; putative RNA polymerase sigma factor 2841424 4607479 azo2564 Azoarcus sp. BH72 putative RNA polymerase sigma factor YP_934068.1 2840924 D 62928 CDS YP_934069.1 119898856 4607480 2841421..2842239 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to BB1303 of Bordetella bronchiseptica of 50% (trembl|Q7WMT7). No domains predicted. TMHMM2 reporting 1 TMH present. No signal peptide present.; Conserved hypothetical protein; hypothetical protein 2842239 4607480 azo2565 Azoarcus sp. BH72 hypothetical protein YP_934069.1 2841421 D 62928 CDS YP_934070.1 119898857 4607481 complement(2842255..2843214) 1 NC_008702.1 Hypothetical protein,53% identity to TrEMBL:Q8YF53,Hypothetical protein BMEI1674 [BMEI1674] [Brucella melitensis] Has IPR000157:TIR domain.; Family membership; ORF6; hypothetical protein 2843214 4607481 azo2566 Azoarcus sp. BH72 hypothetical protein YP_934070.1 2842255 R 62928 CDS YP_934071.1 119898858 4607482 complement(2843306..2844313) 1 NC_008702.1 Putative LysR-family transcriptional regulator protein.62% identity to TrEMBL;Q63NK9,Q8XQV8,Q62B59,Q8VL17. PF00126, Bacterial regulatory helix-turn-helix protein, lysR family;IPR000847, HTH_LysR; Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif. These proteins are very diverse,but for convenience may be grouped into subfamilies on the basis of sequence similarity. One such family, the lysR family, groups together a range of proteins, including ampR, catM, catR, cynR, cysB, gltC, iciA, ilvY, irgB,lysR, metR, mkaC, mleR, nahR, nhaR, nodD, nolR, oxyR,pssR, rbcR, syrM, tcbR, tfdS and trpI. The majority of these proteins appear to be transcription activators and most are known to negatively regulate their own expression. All possess a potential HTH DNA-binding motif towards their N-termini. Has PF03466, LysR substrate binding domain; IPR005119, LysR_subst; The structure of this domain is known and is; LysR family transcriptional regulator 2844313 4607482 azo2567 Azoarcus sp. BH72 LysR family transcriptional regulator YP_934071.1 2843306 R 62928 CDS YP_934072.1 119898859 4607483 2844563..2845765 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family,; Family membership; membrane fusion protein 2845765 4607483 azo2568 Azoarcus sp. BH72 membrane fusion protein YP_934072.1 2844563 D 62928 CDS YP_934073.1 119898860 4607484 2845776..2848967 1 NC_008702.1 AcrB/AcrD/AcrF family protein. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q92U15 (38% identity); TREMBL:Q7NE92 (38% identity). InterPro (IPR000731): Sterol-sensing 5TM box. InterPro (IPR004764): Hydrophobe/amphiphile efflux-1 HAE1. InterPro (IPR001036): Acriflavin resistance protein. Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TMHMM predicting 12 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 2848967 4607484 azo2569 Azoarcus sp. BH72 RND efflux transporter permease YP_934073.1 2845776 D 62928 CDS YP_934074.1 119898861 4607485 2848984..2850510 1 NC_008702.1 Probable outer membrane efflux protein. Homology to opcM from B. cepacia of 40% Component of an efflux system that confers mutiple antibiotic resistence. InterPro: Outer membrane efflux protein Pfam: Outer membrane efflux protein signal peptide no TMHS; Family membership; outer membrane efflux protein 2850510 opcM2 4607485 opcM2 Azoarcus sp. BH72 outer membrane efflux protein YP_934074.1 2848984 D 62928 CDS YP_934075.1 119898862 4609452 complement(2850539..2851069) 1 NC_008702.1 Conserved hypothetical protein. Homology to VV1824 of V.vulnificus of 37% (trembl:Q7MKG9). No domains predicted. No signal peptide or TMH reported present.; hypothetical protein 2851069 4609452 azo2571 Azoarcus sp. BH72 hypothetical protein YP_934075.1 2850539 R 62928 CDS YP_934076.1 119898863 4607486 complement(2851066..2851710) 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted. no signal peptide. 1 TMHs; hypothetical protein 2851710 4607486 azo2572 Azoarcus sp. BH72 hypothetical protein YP_934076.1 2851066 R 62928 CDS YP_934077.1 119898864 4607487 complement(2851794..2853032) 1 NC_008702.1 A gene conferring resistance to fosmidomycin (Fs) encode a putative polypeptide of 406 amino acids (aa) with a molecular weight of 43303 in E.coli. 23% similarity to B. japonicum fsr. A comparison between the aa sequence of Fsr and sequences in a protein database revealed 18% homology to the bacterial drug-export proteins that mediate resistance to tetracycline and chloramphenicol. Hydropathy analysis of the Fsr protein revealed twelve putative transmembrane segments. Fsr does not have any direct effect on the biosynthesis of isoprenoid in E. coli, and that the mechanism for FsR involves the efflux of the drug by a process that is facilitated by Fsr. (Ref: Fujisaki,S. et al., 1996); High confidence in function and specificity; putative fosmidomycin resistance protein 2853032 fsr 4607487 fsr Azoarcus sp. BH72 putative fosmidomycin resistance protein YP_934077.1 2851794 R 62928 CDS YP_934078.1 119898865 4608831 2853125..2853910 1 NC_008702.1 Putative AraC-family transcriptional regulator,; Family membership; AraC family transcriptional regulator 2853910 4608831 azo2574 Azoarcus sp. BH72 AraC family transcriptional regulator YP_934078.1 2853125 D 62928 CDS YP_934079.1 119898866 4607488 complement(2853962..2856457) 1 NC_008702.1 Putative two-component hybrid sensor and regulator,; Family membership; putative two-component hybrid sensor and regulator 2856457 gacS 4607488 gacS Azoarcus sp. BH72 putative two-component hybrid sensor and regulator YP_934079.1 2853962 R 62928 CDS YP_934080.1 119898867 4608854 complement(2856467..2857276) 1 NC_008702.1 catalyzes the reduction of 2,3-dihydrodipicolinate to 2,3,4,5-tetrahydrodipicolinate in lysine and diaminopimelate biosynthesis; dihydrodipicolinate reductase 2857276 dapB 4608854 dapB Azoarcus sp. BH72 dihydrodipicolinate reductase YP_934080.1 2856467 R 62928 CDS YP_934081.1 119898868 4608581 complement(2857314..2857823) 1 NC_008702.1 Putative outer membrane lipoprotein OmlA precursor. Homology to omlA of P. aeruginosa of 33% (sprot|OMLA_PSEAE) MAY HAVE A STRUCTURAL ROLE IN MAINTAINING THE CELL ENVELOPE INTEGRITY. Pfam: SmpA/OmlA family no signal protein 1 TMH; Conserved hypothetical protein; putative outer membrane lipoprotein OmlA 2857823 omlA 4608581 omlA Azoarcus sp. BH72 putative outer membrane lipoprotein OmlA YP_934081.1 2857314 R 62928 CDS YP_934082.1 119898869 4609442 2857844..2858281 1 NC_008702.1 Ferric uptake regulation protein (Ferric uptake regulator)Fur. Acts as a global negative controlling element employing Fe(2+) as a cofactor to bind the operator of the repressed genes. Regulator for biosynthesis of the siderophore alcaligin E. InterPro: Ferric uptake regulator family TIGR00011: conserved hypothetical protein; High confidence in function and specificity; putative ferric uptake regulation protein 2858281 fur 4609442 fur Azoarcus sp. BH72 putative ferric uptake regulation protein YP_934082.1 2857844 D 62928 CDS YP_934083.1 119898870 4608847 complement(2858307..2859974) 1 NC_008702.1 DNA repair protein recN (Recombination protein N). MAY BE INVOLVED IN RECOMBINATIONAL REPAIR OF DAMAGED DNA. recN: DNA repair protein RecN; High confidence in function and specificity; DNA repair protein 2859974 recN 4608847 recN Azoarcus sp. BH72 DNA repair protein YP_934083.1 2858307 R 62928 CDS YP_934084.1 119898871 4609708 complement(2860012..2860896) 1 NC_008702.1 Probable inorganic polyphosphate/ATP-NAD kinase(Poly(P)/ATP NAD kinase). Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. 34% ATP_NADK.InterPro: Domain of unknown function DUF15 Pfam:PF01513; NAD_kinase; 1. TIGR:VC0853.; High confidence in function and specificity; inorganic polyphosphate/ATP-NAD kinase 2860896 ppnK 4609708 ppnK Azoarcus sp. BH72 inorganic polyphosphate/ATP-NAD kinase YP_934084.1 2860012 R 62928 CDS YP_934085.1 119898872 4609613 2861000..2862028 1 NC_008702.1 Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons; heat-inducible transcription repressor 2862028 hrcA 4609613 hrcA Azoarcus sp. BH72 heat-inducible transcription repressor YP_934085.1 2861000 D 62928 CDS YP_934086.1 119898873 4609028 2862075..2863166 1 NC_008702.1 protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic; ferrochelatase 2863166 hemH 4609028 hemH Azoarcus sp. BH72 ferrochelatase YP_934086.1 2862075 D 62928 CDS YP_934087.1 119898874 4608985 2863166..2863522 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs02279 of R. solanacearum of 53% (trembl|Q8Y388(SRS)). No domains predicted. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2863522 4608985 azo2583 Azoarcus sp. BH72 hypothetical protein YP_934087.1 2863166 D 62928 CDS YP_934088.1 119898875 4607489 2863580..2864590 1 NC_008702.1 Similar to TREMBL:Q7NTT5 (32% identity).; hypothetical protein 2864590 4607489 azo2584 Azoarcus sp. BH72 hypothetical protein YP_934088.1 2863580 D 62928 CDS YP_934089.1 119898876 4607490 complement(2864625..2865989) 1 NC_008702.1 Putative serine/threonine kinase,; Family membership; putative protein kinase 2865989 yloP 4607490 yloP Azoarcus sp. BH72 putative protein kinase YP_934089.1 2864625 R 62928 CDS YP_934090.1 119898877 4610192 2866089..2866193 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2866193 4610192 azo2586 Azoarcus sp. BH72 hypothetical protein YP_934090.1 2866089 D 62928 CDS YP_934091.1 119898878 4607491 2866190..2867509 1 NC_008702.1 Catalyzes the hydrolytic cleavage of a carbon-halogen bond in N-ethylammeline; N-ethylammeline chlorohydrolase 2867509 4607491 azo2587 Azoarcus sp. BH72 N-ethylammeline chlorohydrolase YP_934091.1 2866190 D 62928 CDS YP_934092.1 119898879 4607492 2867629..2868345 1 NC_008702.1 Outer membrane protein P.III precursor (Gonococcal protein III) (PIII). InterPro: Bacterial outer membrane protein; High confidence in function and specificity; outer membrane protein A 2868345 ompA1 4607492 ompA1 Azoarcus sp. BH72 outer membrane protein A YP_934092.1 2867629 D 62928 CDS YP_934093.1 119898880 4609443 2868451..2869152 1 NC_008702.1 Involved in ubiquinone biosynthesis; 3-demethylubiquinone-9 3-methyltransferase 2869152 ubiG 4609443 ubiG Azoarcus sp. BH72 3-demethylubiquinone-9 3-methyltransferase YP_934093.1 2868451 D 62928 CDS YP_934094.1 119898881 4610011 complement(2869577..2870845) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA5327 of Azoarcus sp. EbN1 of 44% (gnl|keqq|eba:ebA5327(KEGG)). Pfam: Polysaccharide pyruvyl transferase Pyruvyl-transferases involved in peptidoglycan-associated polymer biosynthesis. no signal peptide. no TMHs; hypothetical protein 2870845 4610011 azo2590 Azoarcus sp. BH72 hypothetical protein YP_934094.1 2869577 R 62928 CDS YP_934095.1 119898882 4607493 2871202..2873805 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002418 of Dechloromonas aromatica of 58% (gi|53730135|ref|ZP_00150721.2|(NBCI ENTREZ)). No domains predicted. No TMHs. signal peptide present.; hypothetical protein 2873805 4607493 azo2591 Azoarcus sp. BH72 hypothetical protein YP_934095.1 2871202 D 62928 CDS YP_934096.1 119898883 4607494 2873818..2874735 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03002419 of Dechloromonas aromatica of 51% (gi|41723832|ref|ZP_00150722.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 2874735 4607494 azo2592 Azoarcus sp. BH72 hypothetical protein YP_934096.1 2873818 D 62928 CDS YP_934097.1 119898884 4607495 2874754..2876877 1 NC_008702.1 conserved hypothetical secreted protein. Homology to Daro03002420 of Dechloromonas aromatica of 39% (gi|41723833|ref|ZP_00150723.1|(NBCI ENTREZ)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2876877 4607495 azo2593 Azoarcus sp. BH72 hypothetical protein YP_934097.1 2874754 D 62928 CDS YP_934098.1 119898885 4607496 2876874..2878430 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV2219 of Chromobacterium violaceum of 36% (trembl|Q7NVX2(SRS)). no domains predicted. no signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 2878430 4607496 azo2594 Azoarcus sp. BH72 hypothetical protein YP_934098.1 2876874 D 62928 CDS YP_934099.1 119898886 4607497 2878452..2879951 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002422 of Dechloromonas aromatica of 63% (gi|41723835|ref|ZP_00150725.1|(NBCI ENTREZ)). No domains predicted. No TMHs. signal peptide present.; Conserved hypothetical protein; hypothetical protein 2879951 4607497 azo2595 Azoarcus sp. BH72 hypothetical protein YP_934099.1 2878452 D 62928 CDS YP_934100.1 119898887 4607498 2879974..2880957 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 2880957 4607498 azo2596 Azoarcus sp. BH72 hypothetical protein YP_934100.1 2879974 D 62928 CDS YP_934101.1 119898888 4607499 2880971..2882566 1 NC_008702.1 Hpyothetical protein with TIR domain. no homology to the data bank. Interpro: TIR domain Pfam: TIR doamin. Site directed mutagenesis and deletion analysis have shown that the TIR domain is essential for Toll and IL-1R activities. Sequence analysis have revealed the presence of three highly conserved regions among the different members of the family: box 1 (FDAFISY), box 2 (GYKLC-RD-PG), and box 3 (a conserved W surrounded by basic residues). It has been proposed that boxes 1 and 2 are involved in the binding of proteins involved in signaling,whereas box 3 is primarily involved in directing localization of receptor, perhaps through interactions with cytoskeletal elements. no signal. peptide 2 TMHs; hypothetical protein 2882566 4607499 azo2597 Azoarcus sp. BH72 hypothetical protein YP_934101.1 2880971 D 62928 CDS YP_934102.1 119898889 4607500 2882757..2884553 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to SMb20495 of S.meliloti of 45% (tremble:Q92W68). Has 3 TPR repeats(IPR001440)(SMART:SM00028):The tetratrico peptide repeat (TPR) is a structural motif present in a wide range of proteins. It mediates protein-protein interactions and the assembly of multiprotein complexes. The TPR motif consists of 3 tandem-repeats of 34 amino acids residues, although individual TPR motifs can be dispersed in the protein sequence. Sequence alignment of the TPR domains reveals a consensus sequence defined by a pattern of small and large amino acids. TPR motifs have been identified in various different organisms, ranging from bacteria to humans. Proteins containing TPRs are involved in a variety of biological processes, such as cell cycle regulation, transcriptional control,mitochondrial and peroxisomal protein transport,neurogenesis and protein folding. No TMH being reported present. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 2884553 4607500 azo2598 Azoarcus sp. BH72 hypothetical protein YP_934102.1 2882757 D 62928 CDS YP_934103.1 119898890 4607501 complement(2884563..2884940) 1 NC_008702.1 Auxin-binding protein 1 precursor(ABP).This is probably a receptor for the plant hormone auxin. 34% Auxin_BP.IPR007113; Cupin_sup.IPR000886;ER_target_S. Pfam:PF02041; Auxin_BP; 1.; Function unclear; putative auxin-binding protein 1 2884940 abp1 4607501 abp1 Azoarcus sp. BH72 putative auxin-binding protein 1 YP_934103.1 2884563 R 62928 CDS YP_934104.1 119898891 4608252 2885097..2885993 1 NC_008702.1 Transcriptional regulator, LysR family This protein activates the transcription of the lysA gene encoding diaminopimelate decarboxylase. LysR is also a negative regulator of its own expression. 37% 1 helixturnhelix PF03466 LysR_substrate; 1. HTH reporting nucleic acid binding motif; Specificity unclear; LysR family transcriptional regulator 2885993 4608252 azo2600 Azoarcus sp. BH72 LysR family transcriptional regulator YP_934104.1 2885097 D 62928 CDS YP_934105.1 119898892 4607502 complement(2886006..2886314) 1 NC_008702.1 Ferredoxin subunits of nitrite reductase. THIS SUBUNIT IS A 2FE-2S FERREDOXIN THAT TRANSFERS ELECTRONS TO IRON SULFUR PROTEIN COMPONENTS (ISP). Similar to nasE,from S.aureus_N315 a naphthalene 12-dioxygenase system ferredoxin component.; High confidence in function and specificity; putative ferredoxin subunit of nitrite reductase 2886314 4607502 azo2601 Azoarcus sp. BH72 putative ferredoxin subunit of nitrite reductase YP_934105.1 2886006 R 62928 CDS YP_934106.1 119898893 4607503 complement(2886484..2886741) 1 NC_008702.1 Translation initiation factor IF-1. No specific function has so far been attributed to this initiation factor; however it seems to stimulate more or less all the activities of the other two initiation factors IF-2 and IF-3.; Family membership; translation initiation factor IF-1 2886741 infA1 4607503 infA1 Azoarcus sp. BH72 translation initiation factor IF-1 YP_934106.1 2886484 R 62928 CDS YP_934107.1 119898894 4609081 complement(2886879..2887082) 1 NC_008702.1 Cold shock-like protein,; High confidence in function and specificity; cold shock-like protein 2887082 4609081 azo2603 Azoarcus sp. BH72 cold shock-like protein YP_934107.1 2886879 R 62928 CDS YP_934108.1 119898895 4607504 2887497..2888339 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ORF62 of Pseudomonas of 60% (trembl|Q936Z8(SRS)). InterPro: Integral membrane protein DUF6. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transporter. Pfam: Integral membrane protein DUF6. probable 9 TMHs. signal peptide; Conserved hypothetical protein; hypothetical protein 2888339 4607504 azo2604 Azoarcus sp. BH72 hypothetical protein YP_934108.1 2887497 D 62928 CDS YP_934109.1 119898896 4607505 complement(2888355..2888876) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cp98 of P. aeruginosa of 33% (tremblnew|AAP22587). no domains predicted. no signal peptide. 2 TMHs.; Conserved hypothetical protein; hypothetical protein 2888876 4607505 azo2605 Azoarcus sp. BH72 hypothetical protein YP_934109.1 2888355 R 62928 CDS YP_934110.1 119898897 4607506 2889090..2889527 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to gsu0250 of G. sulfurreducens of 46% (tremblnew|AAR33584). no domains predicted. no signal peptide. 3 TMHs.; Conserved hypothetical protein; hypothetical protein 2889527 4607506 azo2606 Azoarcus sp. BH72 hypothetical protein YP_934110.1 2889090 D 62928 CDS YP_934111.1 119898898 4607507 complement(2889532..2890509) 1 NC_008702.1 ketopantoate reductase; catalyzes the NADPH reduction of ketopantoate to pantoate; functions in pantothenate (vitamin B5) biosynthesis; 2-dehydropantoate 2-reductase 2890509 panE2 4607507 panE2 Azoarcus sp. BH72 2-dehydropantoate 2-reductase YP_934111.1 2889532 R 62928 CDS YP_934112.1 119898899 4609496 complement(2890560..2891330) 1 NC_008702.1 42% Aldolase_II_N.Class II Aldolase and Adducin N-terminal domain. Pfam: PF00596; Aldolase_II; 1. TIGR: PP2871.; High confidence in function and specificity; aldolase II superfamily protein 2891330 4609496 azo2608 Azoarcus sp. BH72 aldolase II superfamily protein YP_934112.1 2890560 R 62928 CDS YP_934113.1 119898900 4607508 complement(2891520..2892491) 1 NC_008702.1 Conserved hypothetical endonuclease/exonuclease/phosphatase family protein. Homology to RPBDRAFT_0804 of Rhodopseudomonas palustris HaA2 of 315. Pfam: Exo_endo_phos. Endonuclease/Exonuclease/phosphatase family. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signalling. This family includes: AP endonuclease proteins EC:4.2.99.18 e.g APEX1_HUMAN, DNase I proteins EC:3.1.21.1 e.g. DNAS1_HUMAN, Synaptojanin an inositol-1,4,5-trisphosphate phosphatase EC:3.1.3.56 SYNJ1_HUMAN, Sphingomyelinase EC:3.1.4.12 PHL2_BACCE and Nocturnin NOCT_MOUSE. no TMHs. no signal peptide.; Conserved hypothetical protein; endonuclease/exonuclease/phosphatase family protein 2892491 4607508 azo2609 Azoarcus sp. BH72 endonuclease/exonuclease/phosphatase family protein YP_934113.1 2891520 R 62928 CDS YP_934114.1 119898901 4607509 complement(2892605..2894734) 1 NC_008702.1 In E.coli this receptor binds the ferrichrome-iron ligand. It interacts with the tonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin. Signal peptide.; Specificity unclear; putative ferrichrome-iron TonB-dependent receptor 2894734 fhuA2 4607509 fhuA2 Azoarcus sp. BH72 putative ferrichrome-iron TonB-dependent receptor YP_934114.1 2892605 R 62928 CDS YP_934115.1 119898902 4608761 complement(2894837..2895472) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs00511 of R. solanacearum of 32% (trembl|Q8XUW6(SRS)). No domains predicted. singal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 2895472 4608761 azo2611 Azoarcus sp. BH72 hypothetical protein YP_934115.1 2894837 R 62928 CDS YP_934116.1 119898903 4607510 complement(2895707..2896642) 1 NC_008702.1 GAF/GGDEF-domain containing protein,; Conserved hypothetical protein; GAF/GGDEF-domain-containing protein 2896642 4607510 azo2612 Azoarcus sp. BH72 GAF/GGDEF-domain-containing protein YP_934116.1 2895707 R 62928 CDS YP_934117.1 119898904 4607511 complement(2896686..2897087) 1 NC_008702.1 Hypothetical protein, 50% identity (59% simialrity)to TrEMBL;Q92TJ5. Has PF04828, Protein of unknown function (DUF636);IPR006913, GFA;This family of proteins has no known function, but several strongly conserved cysteine residues. Glutathione-dependent formaldehyde-activating enzymes catalyze the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione. All known members of this family contain 5 strongly conserved cysteine residues. NO Signal peptide or TMH present.; Function unclear; hypothetical protein 2897087 4607511 azo2613 Azoarcus sp. BH72 hypothetical protein YP_934117.1 2896686 R 62928 CDS YP_934118.1 119898905 4607512 complement(2897216..2897596) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Saro02001549 of Novosphingobium aromaticivorans of 37% (gi|48849438|ref|ZP_00303681.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2897596 4607512 azo2614 Azoarcus sp. BH72 hypothetical protein YP_934118.1 2897216 R 62928 CDS YP_934119.1 119898906 4607513 complement(2897663..2898382) 1 NC_008702.1 Hypothetical protein,45% similarity to TrEMBL;Q6LHM7. No Good homology over entire length in the DB. No signal Peptide or TMH present.; Function unclear; hypothetical protein 2898382 4607513 azo2615 Azoarcus sp. BH72 hypothetical protein YP_934119.1 2897663 R 62928 CDS YP_934120.1 119898907 4607514 complement(2898514..2898939) 1 NC_008702.1 Puatative Glyoxalase sub unit, 37% identitcal to TrEMBL;Q89MF5 Has PF00903, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily;IPR004360, Gly_bleo_diox: Glyoxalase I, catalyzes the first step of the glyoxal pathway. S-lactoylglutathione is then converted by glyoxalase II to lactic acid . The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric. The sequence of glyoxalase I is well conserved. This domain is found in other related proteins including the Bleomycin resistance protein and dioxygenases eg. 4-hydroxyphenylpyruvate dioxygenase. No SIgnal Peptide or TMH reported present.; Family membership; lactoylglutathione lyase 2898939 4607514 azo2616 Azoarcus sp. BH72 lactoylglutathione lyase YP_934120.1 2898514 R 62928 CDS YP_934121.1 119898908 4607515 complement(2898954..2899577) 1 NC_008702.1 TREMBL:Q89L44: 48% identity.64% similarity. probable haloacid dehalogenase-like hydrolase. InterPro:IPR005834; Hydrolase. Pfam:PF00702; Hydrolase; Phosphoglycolate phosphatase(PGP). TIGR00099: conserved hypothetical prot; Function unclear; HAD superfamily hydrolase 2899577 4607515 azo2617 Azoarcus sp. BH72 HAD superfamily hydrolase YP_934121.1 2898954 R 62928 CDS YP_934122.1 119898909 4607516 complement(2899574..2900011) 1 NC_008702.1 Probable redox-sensitive transcriptional activator,; High confidence in function and specificity; redox-sensitive transcriptional activator 2900011 soxR 4607516 soxR Azoarcus sp. BH72 redox-sensitive transcriptional activator YP_934122.1 2899574 R 62928 CDS YP_934123.1 119898910 4609884 2900083..2900940 1 NC_008702.1 Similar to TREMBL:Q98BY2 (39% identity); TREMBL:Q9A2R1 (40% identity); TREMBL:Q89KG6 (34% identity). Pfam (PF01209): ubiE/COQ5 methyltransferase family.; Function unclear; putative methyltransferase 2900940 4609884 azo2619 Azoarcus sp. BH72 putative methyltransferase YP_934123.1 2900083 D 62928 CDS YP_934124.1 119898911 4607517 complement(2900950..2901330) 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted signal peptide. 3 TMHs; hypothetical protein 2901330 4607517 azo2620 Azoarcus sp. BH72 hypothetical protein YP_934124.1 2900950 R 62928 CDS YP_934125.1 119898912 4607518 complement(2901512..2902048) 1 NC_008702.1 Conserved hypothetical protein. Homology to VPA0111 of V.parahaemolyticus of 62% (trembl:Q87JY7). Has (IPR010323)PF06041, Bacterial protein of unknown function (DUF924);This family consists of several hypothetical bacterial proteins of unknown function. NO signal peptide or TMH present.; hypothetical protein 2902048 4607518 azo2621 Azoarcus sp. BH72 hypothetical protein YP_934125.1 2901512 R 62928 CDS YP_934126.1 119898913 4607519 complement(2902117..2903265) 1 NC_008702.1 Conserved hypothetical membrane protein,; Conserved hypothetical protein; hypothetical protein 2903265 4607519 azo2622 Azoarcus sp. BH72 hypothetical protein YP_934126.1 2902117 R 62928 CDS YP_934127.1 119898914 4607520 complement(2903667..2904227) 1 NC_008702.1 hypothetical protein 2904227 4607520 azo2623 Azoarcus sp. BH72 hypothetical protein YP_934127.1 2903667 R 62928 CDS YP_934128.1 119898915 4607521 2904323..2904757 1 NC_008702.1 Hypothetical truncated transposase. Homology to tnp23 of Azoarcus sp. EbN1 of 86% (gnl|keqq|eba:ebA2478(KEGG)). No domains predicted. No signal peptide. No TMHs.; putative transposase 2904757 4607521 azo2624 Azoarcus sp. BH72 putative transposase YP_934128.1 2904323 D 62928 CDS YP_934129.1 119898916 4607522 2904840..2904950 1 NC_008702.1 Hypothetical truncated transposase. Homology to tnp23 of Azoarcus sp. EbN1 of 87% (gnl|keqq|eba:ebA2478(KEGG)). No domains predicted. No signal peptide. No TMHs.; putative transposase 2904950 4607522 azo2625 Azoarcus sp. BH72 putative transposase YP_934129.1 2904840 D 62928 CDS YP_934130.1 119898917 4607523 2904985..2905281 1 NC_008702.1 Hypothetical truncated transposase. Homology to tnp23 of Azoarcus sp. EbN1 of 86% (gnl|keqq|eba:ebA2478(KEGG)). No domains predicted. No signal peptide. No TMHs.,; Family membership; putative transposase 2905281 4607523 azo2626 Azoarcus sp. BH72 putative transposase YP_934130.1 2904985 D 62928 CDS YP_934131.1 119898918 4607524 complement(2905435..2906151) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Polaromanas sp JS666 of 41% No domain predicted. No signal peptide No TMHs; hypothetical protein 2906151 4607524 azo2627 Azoarcus sp. BH72 hypothetical protein YP_934131.1 2905435 R 62928 CDS YP_934132.1 119898919 4607525 complement(2906296..2906745) 1 NC_008702.1 Hypothetical membrane protein. Homology to yptb3870 of Y. pseudotuberculosis of 29% (tremble:Q664A2). No domains predicted. No signal peptide. 1 TMHs; hypothetical protein 2906745 4607525 azo2628 Azoarcus sp. BH72 hypothetical protein YP_934132.1 2906296 R 62928 CDS YP_934133.1 119898920 4607526 complement(2907060..2907413) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of Exiguobacterium sp. 255-15 (ZP_00355791). No domains predicted. No signal peptide. 2 TMHs; hypothetical protein 2907413 4607526 azo2629 Azoarcus sp. BH72 hypothetical protein YP_934133.1 2907060 R 62928 CDS YP_934134.1 119898921 4607527 complement(2907867..2911886) 1 NC_008702.1 with CobST catalyzes the formation of cobyrinic acid a,c-diamide from hydrogenobyrinic acid a,c-diamide in an ATP-dependent manner; involved in porphyrin and chlorophyll metabolism; vitamin B12 metabolism; cobaltochelatase subunit CobN 2911886 cobN 4607527 cobN Azoarcus sp. BH72 cobaltochelatase subunit CobN YP_934134.1 2907867 R 62928 CDS YP_934135.1 119898922 4607528 complement(2911883..2912239) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE0632 of Nitrosomonas europaea of 42% (trembl|Q82WN6(SRS)). no domains predicted. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 2912239 4607528 azo2631 Azoarcus sp. BH72 hypothetical protein YP_934135.1 2911883 R 62928 CDS YP_934136.1 119898923 4607529 complement(2912250..2912711) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA1924 of P. aeruginosa of 43% (trembl|Q9I2I0(SRS)). No domains predicted. no signal peptide. probable 3TMHS; Conserved hypothetical protein; hypothetical protein 2912711 4607529 azo2632 Azoarcus sp. BH72 hypothetical protein YP_934136.1 2912250 R 62928 CDS YP_934137.1 119898924 4607530 2913014..2913775 1 NC_008702.1 Probable trans-aconitate 2-methyltransferase. Homology to tam of E. coli of 48% (sprot|TAM_ECOLI). Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate (By similarity). Pfam: PTS system, Lactose/Cellobiose specific no signal peptide no TMHs; High confidence in function and specificity; trans-aconitate 2-methyltransferase 2913775 tam 4607530 tam Azoarcus sp. BH72 trans-aconitate 2-methyltransferase YP_934137.1 2913014 D 62928 CDS YP_934138.1 119898925 4609914 complement(2913806..2914276) 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted. 1 TMHs. no signal peptide; hypothetical protein 2914276 4609914 azo2634 Azoarcus sp. BH72 hypothetical protein YP_934138.1 2913806 R 62928 CDS YP_934139.1 119898926 4607531 complement(2914273..2914899) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2914899 4607531 azo2635 Azoarcus sp. BH72 hypothetical protein YP_934139.1 2914273 R 62928 CDS YP_934140.1 119898927 4607532 complement(2915051..2915770) 1 NC_008702.1 TREMBL:Q7MF75: 35% identity; 50% similarity. IPR006323; Phosphonoacetaldehyde hydrolase IPR010976; Beta-phosphoglucomutase hydrolase This group of sequences represent part of one structural subfamily of the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases (IPR005834). The superfamily is defined by the presence of three short catalytic motifs. Hypothetical protein yhcW. InterPro:IPR006402; HAD-SF-IA-v3. IPR005833; Halogease/hydrolase. Pfam: PF00702; Hydrolase Absence of signal peptide (Signal P predicted) and transmembrane helices (TMHMM predicted) IPR005834; Hydrolase. TIGR00099: conserved hypothetical protein; Family membership; putative CbbY family protein 2915770 4607532 azo2636 Azoarcus sp. BH72 putative CbbY family protein YP_934140.1 2915051 R 62928 CDS YP_934141.1 119898928 4607533 complement(2915888..2916406) 1 NC_008702.1 Conserved hypothetical protein. Homology to RSc0334 of R. solanacearum of 59% (sprot:Y334_RALSO). Pfam: Appr-1-p processing enzyme. No signal peptide. No TMHs.; hypothetical protein 2916406 4607533 azo2637 Azoarcus sp. BH72 hypothetical protein YP_934141.1 2915888 R 62928 CDS YP_934142.1 119898929 4607534 complement(2916466..2918697) 1 NC_008702.1 Conserved hypothetical ATPase. Homology to rsc1961 of R. solanacearum of 37% (trembl|Q8XY02). Pfam: Atpase familly associated with various cellular activities (domaine 1 from 300 aa to 499 aa, domaine 2 from 543 to 723 aa). no signal peptide. no TMHs; Conserved hypothetical protein; ATPase 2918697 4607534 azo2638 Azoarcus sp. BH72 ATPase YP_934142.1 2916466 R 62928 CDS YP_934143.1 119898930 4607535 complement(2918694..2919161) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2919161 4607535 azo2639 Azoarcus sp. BH72 hypothetical protein YP_934143.1 2918694 R 62928 CDS YP_934144.1 119898931 4607536 2919238..2920236 1 NC_008702.1 Putative transcriptional factor; putative transcriptional factor 2920236 mdcH 4607536 mdcH Azoarcus sp. BH72 putative transcriptional factor YP_934144.1 2919238 D 62928 CDS YP_934145.1 119898932 4609204 complement(2920329..2921015) 1 NC_008702.1 Hypothetical protein. No homology to the data base. No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2921015 4609204 azo2641 Azoarcus sp. BH72 hypothetical protein YP_934145.1 2920329 R 62928 CDS YP_934146.1 119898933 4607537 complement(2921150..2921314) 1 NC_008702.1 Hypothetical protein, very weak homology with any hits in the database. Has , No domains, repeats, motifs or features could be predicted with confidence above threshold scores.; hypothetical protein 2921314 4607537 azo2642 Azoarcus sp. BH72 hypothetical protein YP_934146.1 2921150 R 62928 CDS YP_934147.1 119898934 4607538 complement(2921344..2921907) 1 NC_008702.1 Putative dual specificity protein phosphatase,; Function unclear; putative dual specificity protein phosphatase 2921907 4607538 azo2643 Azoarcus sp. BH72 putative dual specificity protein phosphatase YP_934147.1 2921344 R 62928 CDS YP_934148.1 119898935 4607539 2922087..2923211 1 NC_008702.1 Putative ADP-ribosyl-[dinitrogen reductase] hydrolase. Homology to draG of R. rubrum of 24% (sprot|DRAG_RHORU) Involved in the regulation of the nitrogen fixation activity by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex. Pfam: ADP-ribosylglycohydrolase no signal peptide no TMHs; High confidence in function and specificity; putative ADP-ribosyl-[dinitrogen reductase] hydrolase 2923211 draG2 4607539 draG2 Azoarcus sp. BH72 putative ADP-ribosyl-[dinitrogen reductase] hydrolase YP_934148.1 2922087 D 62928 CDS YP_934149.1 119898936 4608649 2923248..2923661 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 2923661 4608649 azo2645 Azoarcus sp. BH72 hypothetical protein YP_934149.1 2923248 D 62928 CDS YP_934150.1 119898937 4607540 2923798..2924244 1 NC_008702.1 Conserved hypothetical protein. Homology to Bucepa03000629 of Burkholderia cepacia of 47% (gi|46324491|ref|ZP_00224852.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 2924244 4607540 azo2646 Azoarcus sp. BH72 hypothetical protein YP_934150.1 2923798 D 62928 CDS YP_934151.1 119898938 4607541 complement(2924331..2925149) 1 NC_008702.1 Similar to SWISSPROT:P71505 (50% identity),SWISSPROT:Q8X8L4 (49% identity). Pfam (PF01738): Dienelactone hydrolase family.; High confidence in function and specificity; putative carboxymethylenebutenolidase 2925149 dlhH 4607541 dlhH Azoarcus sp. BH72 putative carboxymethylenebutenolidase YP_934151.1 2924331 R 62928 CDS YP_934152.1 119898939 4608627 complement(2925146..2926453) 1 NC_008702.1 Region start changed from 2926444 to 2926426 (-18 bases); MFS permease 2926453 4608627 azo2648 Azoarcus sp. BH72 MFS permease YP_934152.1 2925146 R 62928 CDS YP_934153.1 119898940 4607542 complement(2926624..2928333) 1 NC_008702.1 GGDEF family protein,; Conserved hypothetical protein; diguanylate cyclase 2928333 4607542 azo2649 Azoarcus sp. BH72 diguanylate cyclase YP_934153.1 2926624 R 62928 CDS YP_934154.1 119898941 4607543 complement(2928487..2928744) 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. TMH in signal peptide; hypothetical protein 2928744 4607543 azo2650 Azoarcus sp. BH72 hypothetical protein YP_934154.1 2928487 R 62928 CDS YP_934155.1 119898942 4607544 complement(2928823..2929257) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD23 of Azoarcus sp. EbN1 of 44% (gnl|keqq|eba:ebD23(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2929257 4607544 azo2651 Azoarcus sp. BH72 hypothetical protein YP_934155.1 2928823 R 62928 CDS YP_934156.1 119898943 4607545 2929286..2929984 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CC0717 of Caulobacter crescentus of 66% (trembl|Q9AA89(SRS)). Also homology to noduline 21 of G. max of 33% (sprot|NO21_SOYBN(SRS)). Has PF01988,(IPR008217)Integral membrane protein DUF125;This family of predicted integral membrane proteins has no known function. However it does include P47818, that may have a role in regulating calcium levels. no signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 2929984 4607545 azo2652 Azoarcus sp. BH72 hypothetical protein YP_934156.1 2929286 D 62928 CDS YP_934157.1 119898944 4607546 2930124..2931599 1 NC_008702.1 Probable low-affinity phosphate transporter protein, PitA. Involved in phosphate transport depending on the proton motive force in E.coli. Similar to Pho-4, a cation-phosphate symporter in N. crassa. InterPro: Phosphate transporter family Signal peptide.; High confidence in function and specificity; putative phosphate transporter 2931599 pitA 4607546 pitA Azoarcus sp. BH72 putative phosphate transporter YP_934157.1 2930124 D 62928 CDS YP_934158.1 119898945 4609583 complement(2931633..2932103) 1 NC_008702.1 Glyoxalase family protein, 54% identity (67% similarity) to TrEMBL;Q88HB6. Has PF00903,Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily;IPR004360, Gly_bleo_diox: Glyoxalase I catalyzes the first step of the glyoxal pathway. S-lactoylglutathione is then converted by glyoxalase II to lactic acid. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric. The sequence of glyoxalase I is well conserved. This domain is found in other related proteins including the Bleomycin resistance protein and dioxygenases eg. 4-hydroxyphenylpyruvate dioxygenase.; Family membership; lactoylglutathione lyase 2932103 4609583 azo2654 Azoarcus sp. BH72 lactoylglutathione lyase YP_934158.1 2931633 R 62928 CDS YP_934159.1 119898946 4607547 2932255..2933628 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein to a protein of similar length. no domains predicted. singal peptie. 6 TMHs; hypothetical protein 2933628 4607547 azo2655 Azoarcus sp. BH72 hypothetical protein YP_934159.1 2932255 D 62928 CDS YP_934160.1 119898947 4607548 2933625..2933957 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to S1719 of Photobacterium profundum of 73% (tremblnew|CAG22368). Has PF02694, Uncharacterized BCR,YnfA/UPF0060 family;IPR003844;This entry describes integral membrane proteins of unknown function. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2933957 4607548 azo2656 Azoarcus sp. BH72 hypothetical protein YP_934160.1 2933625 D 62928 CDS YP_934161.1 119898948 4607549 2934063..2934839 1 NC_008702.1 Ferredoxin-NADP+ reductase (EC 1.18.1.2) Homology to fpr of A. vinelandii of 75% (sprot|FENR_AZOVI). CATALYTIC ACTIVITY: Reduced ferredoxin + NADP(+) = oxidized ferredoxin + NADPH. Interpro: Oxidoreductase FAD and NAD8P)-binding domain (IPR001433); NADH: cytochrome b5 reductase (CBR) (IPR001834) Pfam: Oxidoreductase FAD-binding doamin; Oxidoreductase NAD-binding domain no signal peptide no TMHs; High confidence in function and specificity; ferredoxin-NADP+ reductase 2934839 fpr2 4607549 fpr2 Azoarcus sp. BH72 ferredoxin-NADP+ reductase YP_934161.1 2934063 D 62928 CDS YP_934162.1 119898949 4608825 2934938..2935267 1 NC_008702.1 Putative thioredoxin-disulfide reductase. Homology to trxA (ECs4714) of E. coli of 27%. Participates in various redox reactions through the reversible oxidation of the active center dithiol to a dislufide. InterPro: Thioredoxin (IPR006662) no TMHs; Family membership; putative thioredoxin-disulfide reductase 2935267 trxA2 4608825 trxA2 Azoarcus sp. BH72 putative thioredoxin-disulfide reductase YP_934162.1 2934938 D 62928 CDS YP_934163.1 119898950 4610040 2935396..2935866 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PJS6w01001981 of Polaromonas sp. JS666 of 70% (gi|54030965|ref|ZP_00363100.1|(NBCI ENTREZ)). No domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2935866 4610040 azo2659 Azoarcus sp. BH72 hypothetical protein YP_934163.1 2935396 D 62928 CDS YP_934164.1 119898951 4607550 2935880..2936356 1 NC_008702.1 Hypothetical protein TC0109. TREMBL:Q8XTTO: 64% identity, 75% similarity. This entry describes a family of conserved hypothetical proteins with no known function. InterPro:IPR005247; Cons_hypoth481. IPR008914; PEBP. Pfam: PF01161; PBP Absence of signal peptide. Absence of TMH's; Function unclear; hypothetical protein 2936356 ybcL 4607550 ybcL Azoarcus sp. BH72 hypothetical protein YP_934164.1 2935880 D 62928 CDS YP_934165.1 119898952 4610144 2936540..2937082 1 NC_008702.1 Hypothetical protein which carries at the C-terminus a DnaJ N-terminal domain. InterPro: DnaJ N-terminal domain (IPR001623) Pfam: DnaJ domain no signal peptide no TMHs; hypothetical protein 2937082 4610144 azo2661 Azoarcus sp. BH72 hypothetical protein YP_934165.1 2936540 D 62928 CDS YP_934166.1 119898953 4607551 2937338..2938783 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA2897 of Azoarcus sp. EbN1 of 61% (gnl|keqq|eba:ebA2897(KEGG)). Has PF01936, Protein of unknown function DUF88;(IPR002790). This highly conserved bacterial protein has no known function. The alignment contains many conserved aspartates, suggesting an enzymatic function such as an endonuclease or glycosyl hydrolase (Bateman A pers. obs). Coils2 Program predcit presence of Coiled Coil. No Signal Peptide or TMH present.; hypothetical protein 2938783 4607551 azo2662 Azoarcus sp. BH72 hypothetical protein YP_934166.1 2937338 D 62928 CDS YP_934167.1 119898954 4607552 complement(2938853..2939512) 1 NC_008702.1 Conserve peroxiredoxin. Homology to mll2432 of M. loti of 74% (trembl|Q98IF0). Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic fatty acid and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Pfam: AhpC/TSA family no TMHs. No signal peptide.; Family membership; peroxiredoxin 2939512 4607552 azo2663 Azoarcus sp. BH72 peroxiredoxin YP_934167.1 2938853 R 62928 CDS YP_934168.1 119898955 4607553 2939583..2939930 1 NC_008702.1 Conserved hypothetical protein. Homology to TdenA01001041 of Thiobacillus denitrificans of 39% (gi|52007163|ref|ZP_00334541.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 2939930 4607553 azo2664 Azoarcus sp. BH72 hypothetical protein YP_934168.1 2939583 D 62928 CDS YP_934169.1 119898956 4607554 complement(2939940..2940662) 1 NC_008702.1 Hypothetical secreted protein. No homology of the entire protein to the data bank. no domains predicted signal peptide no TMHs; hypothetical protein 2940662 4607554 azo2665 Azoarcus sp. BH72 hypothetical protein YP_934169.1 2939940 R 62928 CDS YP_934170.1 119898957 4607555 complement(2940920..2941666) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Signal peptide present.; hypothetical protein 2941666 4607555 azo2666 Azoarcus sp. BH72 hypothetical protein YP_934170.1 2940920 R 62928 CDS YP_934171.1 119898958 4607556 2941903..2942196 1 NC_008702.1 Putative transcriptional repressor,; High confidence in function and specificity; putative transcriptional repressor 2942196 nolR 4607556 nolR Azoarcus sp. BH72 putative transcriptional repressor YP_934171.1 2941903 D 62928 CDS YP_934172.1 119898959 4609393 2942246..2943358 1 NC_008702.1 Probable glycerol trinitrate reductase. Homology to nerA of A. radiobacter of 66% (trembl|O31246) InterPro: NADH:flavin oxidoreductase/NADH oxidase (IPR001155) Pfam: NADH:flavin oxidoreductase/NADH oxidase no signal peptide no TMHs; High confidence in function and specificity; glycerol trinitrate reductase 2943358 nerA 4609393 nerA Azoarcus sp. BH72 glycerol trinitrate reductase YP_934172.1 2942246 D 62928 CDS YP_934173.1 119898960 4609353 complement(2943448..2944503) 1 NC_008702.1 Conserved hypothetical cytochrome c family protein. Homology to bra0353 of B. suis of 50% (trembl|Q8FWU2). InterPro: Cytochrome c class I (IPR003088). Pfam: cytochrome c. signal peptide. no TMHS; Function unclear; cytochrome c family protein 2944503 4609353 azo2669 Azoarcus sp. BH72 cytochrome c family protein YP_934173.1 2943448 R 62928 CDS YP_934174.1 119898961 4607557 2944870..2945280 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to gsu0710 of G. sulfurreducens of 66% (tremblnew|AAR34040). no doamins predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 2945280 4607557 azo2670 Azoarcus sp. BH72 hypothetical protein YP_934174.1 2944870 D 62928 CDS YP_934175.1 119898962 4607558 complement(2945296..2945943) 1 NC_008702.1 Transcriptional regulatory protein,; Specificity unclear; two-component response regulator 2945943 fixJ1 4607558 fixJ1 Azoarcus sp. BH72 two-component response regulator YP_934175.1 2945296 R 62928 CDS YP_934176.1 119898963 4608766 complement(2945927..2947732) 1 NC_008702.1 Conserved hypothetical sensor histidine kinase,MODULATES THE ACTIVITY OF FIXJ A TRANSCRIPTIONAL ACTIVATOR OF NITROGEN FIXATION FIXK GENE. FIXL PROBABLY ACTS AS A KINASE THAT PHOSPHORYLATES FIXJ.; Specificity unclear; putative sensor histidine kinase 2947732 4608766 azo2672 Azoarcus sp. BH72 putative sensor histidine kinase YP_934176.1 2945927 R 62928 CDS YP_934177.1 119898964 4607559 2947962..2948441 1 NC_008702.1 Hypothetical protein,69% Identity (75% similarity) to TrEMBl;Q8XS84 Has PF06983, 3-demethylubiquinone-9 3-methyltransferase;IPR009725 3-dmu-93-mtfrase;This family represents a conserved region approximately 100 residues long within a number of bacterial and archaeal 3-demethylubiquinone-9 3-methyltransferases (EC:2.1.1.64). Note that some family members contain more than one copy of this region, and that many members are hypothetical proteins.; Function unclear; hypothetical protein 2948441 4607559 azo2673 Azoarcus sp. BH72 hypothetical protein YP_934177.1 2947962 D 62928 CDS YP_934178.1 119898965 4607560 complement(2949219..2950808) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to Bcep02002160 of Burkholderia fungorum of 35% (gi|48787369|ref|ZP_00283451.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. 6 TMHs; Conserved hypothetical protein; hypothetical protein 2950808 4607560 azo2674 Azoarcus sp. BH72 hypothetical protein YP_934178.1 2949219 R 62928 CDS YP_934179.1 119898966 4607561 complement(2950840..2951982) 1 NC_008702.1 catalyzes the formation of dTDP-D-fucosamine from dTDP-4-oxo-6-deoxy-D-glucose in enterobacterial common antigen biosynthesis; TDP-4-oxo-6-deoxy-D-glucose transaminase 2951982 4607561 azo2675 Azoarcus sp. BH72 TDP-4-oxo-6-deoxy-D-glucose transaminase YP_934179.1 2950840 R 62928 CDS YP_934180.1 119898967 4607562 complement(2952049..2952921) 1 NC_008702.1 Similar to TREMBL:Q82SX7 (48% identity, 66% similarity, generic methyl transferase). Pfam (PF01209): ubiE/COQ5 methyltransferase family. TIGRFAM (TIGR00091): conserved hypothetical protein. TMHMM reporting one transmembrane helix.; Function unclear; putative methyltransferase 2952921 4607562 azo2676 Azoarcus sp. BH72 putative methyltransferase YP_934180.1 2952049 R 62928 CDS YP_934181.1 119898968 4607563 complement(2952918..2953337) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE2170 of Nitrosomonas europaea of 44% (trembl|Q82SX6(SRS)). Pfam: GtrA-like protein. Members of this family are predicted to be integral membrane proteins with three or four transmembrane spans. They are involved in the synthesis of cell surface polysaccharides. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 2953337 4607563 azo2677 Azoarcus sp. BH72 hypothetical protein YP_934181.1 2952918 R 62928 CDS YP_934182.1 119898969 4607564 complement(2953334..2954254) 1 NC_008702.1 hypothetical protein 2954254 4607564 azo2678 Azoarcus sp. BH72 hypothetical protein YP_934182.1 2953334 R 62928 CDS YP_934183.1 119898970 4607565 complement(2954285..2954947) 1 NC_008702.1 InterPro: Bacterial transferase hexapeptide repeat; Function unclear; acyltransferase 2954947 4607565 azo2679 Azoarcus sp. BH72 acyltransferase YP_934183.1 2954285 R 62928 CDS YP_934184.1 119898971 4607566 complement(2954944..2955885) 1 NC_008702.1 Bactoprenol glucosyl transferase (EC 2.4.1.-). Involved in O antigen modification. Catalyzes the transfer of the glucose residue from UDP-glucose to a lipid carrier (By similarity).; Specificity unclear; glycosyltransferase 2955885 4607566 azo2680 Azoarcus sp. BH72 glycosyltransferase YP_934184.1 2954944 R 62928 CDS YP_934185.1 119898972 4607567 complement(2955882..2956574) 1 NC_008702.1 High confidence in function and specificity; hypothetical protein 2956574 4607567 azo2681 Azoarcus sp. BH72 hypothetical protein YP_934185.1 2955882 R 62928 CDS YP_934186.1 119898973 4607568 2957374..2957898 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 2957898 4607568 azo2682 Azoarcus sp. BH72 hypothetical protein YP_934186.1 2957374 D 62928 CDS YP_934187.1 119898974 4607569 2957907..2958251 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 2958251 4607569 azo2683 Azoarcus sp. BH72 hypothetical protein YP_934187.1 2957907 D 62928 CDS YP_934188.1 119898975 4607570 complement(2958597..2959409) 1 NC_008702.1 putative prophage integrase; Family membership; putative integrase 2959409 4607570 azo2684 Azoarcus sp. BH72 putative integrase YP_934188.1 2958597 R 62928 CDS YP_934189.1 119898976 4608238 2960329..2962404 1 NC_008702.1 GGDEF/EAL/PAS-domain containing protein; diguanylate cyclase 2962404 4608238 azo2685 Azoarcus sp. BH72 diguanylate cyclase YP_934189.1 2960329 D 62928 CDS YP_934190.1 119898977 4610021 2962857..2963009 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide present. TMH in signal peptide.; hypothetical protein 2963009 4610021 azo2687 Azoarcus sp. BH72 hypothetical protein YP_934190.1 2962857 D 62928 CDS YP_934191.1 119898978 4607573 complement(2963024..2963503) 1 NC_008702.1 Putative periplasmic protein [creA],59% identity (77% similarity) to SwissProt;P08367. TrEMBL; Q8XZD0(59% identity). Has PF05981, CreA protein;IPR010292, CreA: This family consists of several bacterial CreA proteins, the function of which is unknown.; High confidence in function and specificity; hypothetical protein 2963503 creA 4607573 creA Azoarcus sp. BH72 hypothetical protein YP_934191.1 2963024 R 62928 CDS YP_934192.1 119898979 4608529 complement(2963500..2963913) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001159 of Dechloromonas aromatica of 50% (gi|41726181|ref|ZP_00152939.1|(NBCI ENTREZ)). Pfam: ProQ activator of osmoprotectant transpoter ProP. This family includes ProQ, which is required for full activation of the osmoprotectant transporter ProQ, in Escherichia coli. no signal. peptide no TMHs; hypothetical protein 2963913 4608529 azo2689 Azoarcus sp. BH72 hypothetical protein YP_934192.1 2963500 R 62928 CDS YP_934193.1 119898980 4607574 2964056..2965018 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA7139 of Azoarcus sp. EbN1 of 64% (gnl|keqq|eba:ebA7139(KEGG)). No domains predicted. No signal peptide. No TMH present.; hypothetical protein 2965018 4607574 azo2690 Azoarcus sp. BH72 hypothetical protein YP_934193.1 2964056 D 62928 CDS YP_934194.1 119898981 4607575 2965210..2967042 1 NC_008702.1 GGDEF family protein,; Conserved hypothetical protein; diguanylate cyclase 2967042 4607575 azo2691 Azoarcus sp. BH72 diguanylate cyclase YP_934194.1 2965210 D 62928 CDS YP_934195.1 119898982 4607576 2967068..2968882 1 NC_008702.1 GGDEF family protein; diguanylate cyclase 2968882 4607576 azo2692 Azoarcus sp. BH72 diguanylate cyclase YP_934195.1 2967068 D 62928 CDS YP_934196.1 119898983 4607577 2969110..2970591 1 NC_008702.1 Flagellin. Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. InterPro: Flagellin N-terminus, Flagellin C-terminus Pfam: Bacterial flagellin N-terminus, bacterial flagellin C-terminus no signal peptide no TMH; High confidence in function and specificity; flagellin 2970591 fliC2 4607577 fliC2 Azoarcus sp. BH72 flagellin YP_934196.1 2969110 D 62928 CDS YP_934197.1 119898984 4608796 2970739..2971869 1 NC_008702.1 InterPro: DegT/DnrJ/EryC1/StrS family; Specificity unclear; nucleotide sugar aminotransferase 2971869 4608796 azo2694 Azoarcus sp. BH72 nucleotide sugar aminotransferase YP_934197.1 2970739 D 62928 CDS YP_934198.1 119898985 4607578 2971926..2973140 1 NC_008702.1 Conserved hypothetical protein. Homology to BF2580 of Bacteroides fragilis of 47% (gnl|keqq|bfr:BF2580(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2973140 4607578 azo2695 Azoarcus sp. BH72 hypothetical protein YP_934198.1 2971926 D 62928 CDS YP_934199.1 119898986 4607579 2973203..2974009 1 NC_008702.1 Conserved hypothetical protein. Homology to Mmc102000919 of Magnetococcus sp. MC-1 of 48% (gi|48833619|ref|ZP_00290637.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 2974009 4607579 azo2696 Azoarcus sp. BH72 hypothetical protein YP_934199.1 2973203 D 62928 CDS YP_934200.1 119898987 4607580 2974127..2975602 1 NC_008702.1 Hypothetical protein. No homology of the entire protein with the data base. InterPro: Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose,dolichol phosphate and teichoic acids. No signal peptide. No TMHs; hypothetical protein 2975602 4607580 azo2697 Azoarcus sp. BH72 hypothetical protein YP_934200.1 2974127 D 62928 CDS YP_934201.1 119898988 4607581 2975648..2976952 1 NC_008702.1 Phosphoenolpyruvate phosphomutase precursor(Phosphoenolpyruvate mutase) (PEP mutase) (PEP phosphomutase).FORMATION OF A CARBON-PHOSPHORUS BOND BY CONVERTING PHOSPHOENOLPYRUVATE (PEP) TO PHOSPHONOPYRUVATE (P-PYR). 34% Isocit_lyase_ph.; High confidence in function and specificity; putative phosphoenolpyruvate phosphomutase 2976952 pepM 4607581 pepM Azoarcus sp. BH72 putative phosphoenolpyruvate phosphomutase YP_934201.1 2975648 D 62928 CDS YP_934202.1 119898989 4609521 2976949..2978073 1 NC_008702.1 3-phosphonopyruvate decarboxylase (EC 4.1.1.-). InterPro: Thiamine pyrophosphate dependent enzyme acolac_lg: acetolactate synthase large subunit,biosynthetic type; Family membership; phosphonopyruvate decarboxylase 2978073 4609521 azo2699 Azoarcus sp. BH72 phosphonopyruvate decarboxylase YP_934202.1 2976949 D 62928 CDS YP_934203.1 119898990 4607582 2978078..2979187 1 NC_008702.1 Conserved hypothetical alcohol dehydrogenase. Homology to bp2820 of B. pertussis of 29% (TREMBL:Q7VV69). InterPro: Iron-containing alcohol dehydrogenase (IPR001670). Pfam: Iron-containing alcohol dehydrogenase. no signal peptide. no TMHs.; Family membership; alcohol dehydrogenase 2979187 4607582 azo2700 Azoarcus sp. BH72 alcohol dehydrogenase YP_934203.1 2978078 D 62928 CDS YP_934204.1 119898991 4607583 2979203..2980453 1 NC_008702.1 Putative CDP-glycerol:glycerophosphate glycerophosphotransferase tarF (EC 2.7.8.-). Catalyzes the addition of a second single glycerol-P residue to the prenolpyrophosphate-linked dissacharide to complete the linkage unit (Potential). PurN: phosphoribosylglycinamide formy; Family membership; teichoic acid biosynthesis protein B 2980453 4607583 azo2701 Azoarcus sp. BH72 teichoic acid biosynthesis protein B YP_934204.1 2979203 D 62928 CDS YP_934205.1 119898992 4607584 2980491..2984042 1 NC_008702.1 InterPro: Glycosyl transferase family 2; Family membership; glycosyltransferase 2984042 4607584 azo2702 Azoarcus sp. BH72 glycosyltransferase YP_934205.1 2980491 D 62928 CDS YP_934206.1 119898993 4607585 complement(2984043..2986367) 1 NC_008702.1 Conserved hypothetical protein. Homology to magn028431 of M. magnetotacticum of 33% (ZP 00053827). Domain structur: 76 aa - 144 aa TPR; 201 aa - 316 aa TPR; 408 aa - 761 aa COG3914. InterPro: TPR repeat (IPR001440). no signal peptide no TMH.; hypothetical protein 2986367 4607585 azo2703 Azoarcus sp. BH72 hypothetical protein YP_934206.1 2984043 R 62928 CDS YP_934207.1 119898994 4607586 2986580..2988061 1 NC_008702.1 Flagellin. Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. InterPro: Flagellin N-terminus, Flagellin C-terminus Pfam: Bacterial flagellin N-terminus, bacterial flagellin C-terminus no TMH no signal peptide; High confidence in function and specificity; flagellin 2988061 fliC3 4607586 fliC3 Azoarcus sp. BH72 flagellin YP_934207.1 2986580 D 62928 CDS YP_934208.1 119898995 4608797 2988140..2988544 1 NC_008702.1 Protein flaG. Although these proteins are known to be important for flagellar their exact function is unknown TREMBL:Q82UA2: 56% identity, 79% similarity. InterPro:IPR005186; FlaG. Pfam:PF03646; FlaG L12: ribosomal protein L7/L12 Nonsecretory protein with low signal peptide probability (0.041): SignalP predicted Transmembrane helices 0; High confidence in function and specificity; flagellar protein 2988544 flaG 4608797 flaG Azoarcus sp. BH72 flagellar protein YP_934208.1 2988140 D 62928 CDS YP_934209.1 119898996 4608769 2988571..2989992 1 NC_008702.1 Flagellar hook-associated protein 2 (HAP2) (Filament cap protein) (Flagellar cap protein). The flagellar hook-associated protein 2 (AHP2 or FilD) is a capping protein for the flagella and forms the distal end of the flagella. The protein plays a role in mucin specific adhesion of the bacteria. InterPro: Flagellar hook-associated protein 2 no signal-peptide no TMHs; High confidence in function and specificity; flagellar hook-associated protein 2989992 fliD 4608769 fliD Azoarcus sp. BH72 flagellar hook-associated protein YP_934209.1 2988571 D 62928 CDS YP_934210.1 119898997 4608798 2990004..2990414 1 NC_008702.1 Flagellar protein fliS. The function of this protein in flagellar biosynthesis is unknown, but appears to be regulatory. Might be a chaperon for Flic. InterPro: Flagellar protein FliS fliS: flagellar protein FliS Pfam: Flagellar protein FliS no signal peptide no TMHs; Family membership; flagellar protein FliS 2990414 fliS 4608798 fliS Azoarcus sp. BH72 flagellar protein FliS YP_934210.1 2990004 D 62928 CDS YP_934211.1 119898998 4608812 2990427..2990744 1 NC_008702.1 Hypothetical lagellar related protein FliT. Homology to fliT of R. solanacearum of 27% (trembl|Q8XST2). no domains predicted. no signal peptide. no TMHs.; flagellar-like protein FliT 2990744 fliT 4608812 fliT Azoarcus sp. BH72 flagellar-like protein FliT YP_934211.1 2990427 D 62928 CDS YP_934212.1 119898999 4608813 2990776..2991891 1 NC_008702.1 Hypothetical protein,42% similarity with TrEMBL;Q82T52. No Signal Peptide or TMH reported present.; Family membership; hypothetical protein 2991891 4608813 azo2709 Azoarcus sp. BH72 hypothetical protein YP_934212.1 2990776 D 62928 CDS YP_934213.1 119899000 4607587 2991881..2992258 1 NC_008702.1 Conserved hypothetical flagellar related protein. Homology to NE2078 of Nitrosomonas europaea of 48% (CAD85989). Tirgfam: flhB_rel(TIGR). This group describes a short protein (80-93 residues) homologous to the C-terminus of the flagellar biosynthetic protein FlhB. It is found so far only in species that also have FlhB. In a phylogenetic tree based on alignment of both this family and the homologous region of FlhB and its homologs, the members of this family form a monophyletic set. No TMHs. No signal peptide.; Conserved hypothetical protein; flagellar related protein 2992258 4607587 azo2710 Azoarcus sp. BH72 flagellar related protein YP_934213.1 2991881 D 62928 CDS YP_934214.1 119899001 4607588 2992357..2993136 1 NC_008702.1 Conserved hypothetical protein,55% similarity to TrEMBL;Q82T50. Has IPR009926;PF07317:YcgR protein;This family consists of several hypothetical YcgR proteins. YcgR may be involved in the flagellar motor function and may be a new member of the flagellar regulon. No signal peptide or TMH reported present.; Function unclear; hypothetical protein 2993136 4607588 azo2711 Azoarcus sp. BH72 hypothetical protein YP_934214.1 2992357 D 62928 CDS YP_934215.1 119899002 4607589 2993380..2997147 1 NC_008702.1 GGDEF/PAS/PAC-domain containing protein,; Conserved hypothetical protein; diguanylate cyclase 2997147 4607589 azo2712 Azoarcus sp. BH72 diguanylate cyclase YP_934215.1 2993380 D 62928 CDS YP_934216.1 119899003 4607590 complement(2997226..2997522) 1 NC_008702.1 Region start changed from 2997519 to 2997495 (-24 bases); flagellar hook-basal body complex protein FliE 2997522 4607590 azo2713 Azoarcus sp. BH72 flagellar hook-basal body complex protein FliE YP_934216.1 2997226 R 62928 CDS YP_934217.1 119899004 4607591 complement(2997698..2999176) 1 NC_008702.1 Sigma-54 dependent response regulator,; High confidence in function and specificity; sigma-54 dependent response regulator 2999176 fleR 4607591 fleR Azoarcus sp. BH72 sigma-54 dependent response regulator YP_934217.1 2997698 R 62928 CDS YP_934218.1 119899005 4608774 complement(2999173..3000354) 1 NC_008702.1 Putative sensory box sensor histidine kinase,; High confidence in function and specificity; putative sensory box sensor histidine kinase 3000354 fleS 4608774 fleS Azoarcus sp. BH72 putative sensory box sensor histidine kinase YP_934218.1 2999173 R 62928 CDS YP_934219.1 119899006 4608775 3000514..3002202 1 NC_008702.1 the MS-ring anchors the flagellum to the cytoplasmic membrane; part of the flagellar basal body which consists of four rings L, P, S, and M mounted on a central rod; flagellar MS-ring protein 3002202 fliF 4608775 fliF Azoarcus sp. BH72 flagellar MS-ring protein YP_934219.1 3000514 D 62928 CDS YP_934220.1 119899007 4608799 3002199..3003197 1 NC_008702.1 Flagellar motor switch protein fliG. FLIG IS ONE OF THREE PROTEINS (FLIG FLIN FLIM) THAT FORM A SWITCH COMPLEX THAT IS PROPOSED TO BE LOCATED AT THE BASE OF THE BASAL BODY. THIS COMPLEX INTERACTS WITH THE CHEY AND CHEZ CHEMOTAXIS PROTEINS IN ADDITION TO CONTACTING COMPONENTS OF THE MOTOR THAT DETERMINE THE DIRECTION OF FLAGELLAR ROTATION (BY SIMILARITY). fliG: flagellar motor switch protein Pfam: fliG C-terminal domain no signal peptide no TMHs; High confidence in function and specificity; flagellar motor switch protein FliG 3003197 fliG 4608799 fliG Azoarcus sp. BH72 flagellar motor switch protein FliG YP_934220.1 3002199 D 62928 CDS YP_934221.1 119899008 4608800 3003231..3003977 1 NC_008702.1 Probable Flagellar assembly protein FliH, 38% identity to TrEMBL;Q7VYE7, Q7WJB3, Q8XSS5. Has PF02108,Flagellar assembly protein FliH;IPR000563, Flag_FliH; Many flagellar proteins are exported by a flagellum-specific export pathway. Attempts have been made to characterise the apparatus responsible for this process, by designing assays to screen for mutants with export defects. Experiments involving filament removal from temperature-sensitive flagellar mutants of Salmonella typhimurium have shown that, while most mutants were able to regrow filaments, flhA, fliH, fliI and fliN mutants showed no or greatly reduced regrowth. This suggests that the corresponding gene products are involved in the process of flagellum-specific export. The sequence of fliH has been deduced and shown to encode a protein of molecular mass of 25,782 Da.; flagellar assembly protein FliH 3003977 fliH 4608800 fliH Azoarcus sp. BH72 flagellar assembly protein FliH YP_934221.1 3003231 D 62928 CDS YP_934222.1 119899009 4608801 3003991..3005397 1 NC_008702.1 ATP synthase (EC 3.6.3.14). Probable catalytic subunit of a protein translocase for flagellum-specific export or a proton translocase involved in local circuits at the flagellum. May be involved in a specialized protein export pathway that proceeds without signal peptide cleavage. InterPro: ATP synthase alpha and beta subunit central region Pfam: ATP synthase alpha/beta family no signal peptide no TMHs; High confidence in function and specificity; ATP synthase 3005397 fliI 4608801 fliI Azoarcus sp. BH72 ATP synthase YP_934222.1 3003991 D 62928 CDS YP_934223.1 119899010 4608802 3005493..3005945 1 NC_008702.1 Flagellar fliJ protein. FLAGELLAR PROTEIN THAT AFFECTS CHEMOTACTIC EVENTS. Might have a chaperon-like activity InterPro: Flagellar FliJ protein no signal peptide no TMHs; Family membership; putative flagellar protein FliJ 3005945 fliJ 4608802 fliJ Azoarcus sp. BH72 putative flagellar protein FliJ YP_934223.1 3005493 D 62928 CDS YP_934224.1 119899011 4608803 3006109..3007209 1 NC_008702.1 Flagellar hook-length control protein. Controls the length of the flagellar hook. no TMHs no signal peptide; Family membership; putative flagellar hook-length control protein FliK 3007209 fliK 4608803 fliK Azoarcus sp. BH72 putative flagellar hook-length control protein FliK YP_934224.1 3006109 D 62928 CDS YP_934225.1 119899012 4608804 3007300..3007881 1 NC_008702.1 putative flagellar basal body-associated protein FliL Controls the rotational direction of flagella during chemotaxis. no signal peptide no TMHs; Family membership; putative flagellar basal body-associated protein FliL 3007881 fliL 4608804 fliL Azoarcus sp. BH72 putative flagellar basal body-associated protein FliL YP_934225.1 3007300 D 62928 CDS YP_934226.1 119899013 4608805 3007896..3008906 1 NC_008702.1 with FliG and FliN makes up the switch complex which is involved in switching the direction of the flagella rotation; flagellar motor switch protein FliM 3008906 fliM 4608805 fliM Azoarcus sp. BH72 flagellar motor switch protein FliM YP_934226.1 3007896 D 62928 CDS YP_934227.1 119899014 4608806 3008899..3009378 1 NC_008702.1 Flagellar motor switch protein fliN (Flagellar motor switch protein mopA) (Fragment). FliN is one of three proteins (FliG FliN FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins in addition to contacting components of the motor that determine the dirction of flagellar rotation (by similarity). InterPro: Surface presentation of antigens (SPOA) protein, Flagellar motor switch FliN protein Pfam: Surface presentation of antigens (SPOA) protein no signal peptide no TMHs; High confidence in function and specificity; flagellar motor switch FliN 3009378 fliN 4608806 fliN Azoarcus sp. BH72 flagellar motor switch FliN YP_934227.1 3008899 D 62928 CDS YP_934228.1 119899015 4608807 3009540..3009863 1 NC_008702.1 Flagellar biosynthesis protein,FliO, 26% identity to TrEMBL;Q8XBA2, Q83ML3. SProt;P22586 Has Signal peptide. Has PF04347, Flagellar biosynthesis protein,FliO;IPR007442 ; FliO is an essential component of the flagellum-specific protein export apparatus. It is an integral membrane protein. Its precise molecular function is unknown.; flagellar biosynthesis protein FliO 3009863 fliO 4608807 fliO Azoarcus sp. BH72 flagellar biosynthesis protein FliO YP_934228.1 3009540 D 62928 CDS YP_934229.1 119899016 4608808 3009853..3010629 1 NC_008702.1 FliP, with proteins FliQ and FliR, forms the core of the central channel in the flagella export apparatus; flagellar biosynthesis protein FliP 3010629 fliP 4608808 fliP Azoarcus sp. BH72 flagellar biosynthesis protein FliP YP_934229.1 3009853 D 62928 CDS YP_934230.1 119899017 4608809 3010643..3010912 1 NC_008702.1 with proteins FliP and FliR forms the core of the central channel in the flagella export apparatus; flagellar biosynthesis protein FliQ 3010912 fliQ 4608809 fliQ Azoarcus sp. BH72 flagellar biosynthesis protein FliQ YP_934230.1 3010643 D 62928 CDS YP_934231.1 119899018 4608810 3010935..3011747 1 NC_008702.1 Flagellar biosynthesis protein fliR. Role in flagellar biosynthesis. InterPro: Bacterial export protein family 1 PFam: Bacterial export proteins, family 1 no signal peptide most probable 8 TMHs; High confidence in function and specificity; flagellar biosynthesis protein FliR 3011747 fliR 4608810 fliR Azoarcus sp. BH72 flagellar biosynthesis protein FliR YP_934231.1 3010935 D 62928 CDS YP_934232.1 119899019 4608811 complement(3011751..3012236) 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted signal peptide no TMHs; hypothetical protein 3012236 4608811 azo2729 Azoarcus sp. BH72 hypothetical protein YP_934232.1 3011751 R 62928 CDS YP_934233.1 119899020 4607592 complement(3012247..3013509) 1 NC_008702.1 Flagellar hook-associated protein 3 (HAP3) (Hook-filament junction protein). no signal peptide no TMHs; High confidence in function and specificity; flagellar hook-filament junction protein 3 3013509 flgL 4607592 flgL Azoarcus sp. BH72 flagellar hook-filament junction protein 3 YP_934233.1 3012247 R 62928 CDS YP_934234.1 119899021 4608787 complement(3013521..3015512) 1 NC_008702.1 flagellar hook-filament junction protein 1 (HAP1). no signal peptide no TMHs; High confidence in function and specificity; flagellar hook-filament junction protein 1 3015512 flgK 4608787 flgK Azoarcus sp. BH72 flagellar hook-filament junction protein 1 YP_934234.1 3013521 R 62928 CDS YP_934235.1 119899022 4608786 complement(3015668..3016735) 1 NC_008702.1 Peptidoglycan hydrolase flgJ (EC 3.2.1.-) (Muramidase flgJ). Flagellum-specific muramidase which hydrolyses the peptidoglycan layer to assemble the rod structure in the periplasmic space (by similarity). InterPro: Mannosyl-glycoprotein endo-beta-N-acetylglucosamidases Pfam: Mannosyl-glycoprotein endo-beta-N-acetylglucosamidases no signal peptide no TMHs; High confidence in function and specificity; peptidoglycan hydrolase 3016735 flgJ 4608786 flgJ Azoarcus sp. BH72 peptidoglycan hydrolase YP_934235.1 3015668 R 62928 CDS YP_934236.1 119899023 4608785 complement(3016818..3017930) 1 NC_008702.1 part of the basal body which consists of four rings L, P, S, and M mounted on a central rod; Vibrio parahaemolyticus, Yersinia, Bradyrhizobium and other bacteria have two copies of this and other flagellar genes; the V. parahaemolyticus protein is associated with the polar flagella and the Bradyrhizobium protein is associated with the thick flagellum; flagellar basal body P-ring protein 3017930 flgI 4608785 flgI Azoarcus sp. BH72 flagellar basal body P-ring protein YP_934236.1 3016818 R 62928 CDS YP_934237.1 119899024 4608784 complement(3017950..3018627) 1 NC_008702.1 Flagellar L-ring protein precursor (Basal body L-ring protein). Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation. The L ring reside in the outer membrane. FlgH, which is exported across the cell membrane to their destinations in the outer membrane, has a typical N-terminal cleaved signal-peptide sequences and is predicted to have an extensive -sheet structure, in keeping with other outer membrane proteins. Pfam: Flagellar L-ring protein signal peptide no TMHs; High confidence in function and specificity; flagellar L-ring protein 3018627 flgH 4608784 flgH Azoarcus sp. BH72 flagellar L-ring protein YP_934237.1 3017950 R 62928 CDS YP_934238.1 119899025 4608783 complement(3018650..3019432) 1 NC_008702.1 makes up the distal portion of the flagellar basal body rod; flagellar basal body rod protein FlgG 3019432 flgG 4608783 flgG Azoarcus sp. BH72 flagellar basal body rod protein FlgG YP_934238.1 3018650 R 62928 CDS YP_934239.1 119899026 4608782 complement(3019571..3020308) 1 NC_008702.1 Flagellar basal-body rod protein flgF (Putative proximal rod protein). The rod has been shown to consist of four different, yet evolutionary related proteins: in the distal portion of the rod there are about 26 subunits of protein flgG and in the proximal portion there are about six subunits each of proteins flgB, flgC, and flgF. These four proteins contain a highly conserved asparagine-rich domain at their N terminus. InterPro: Flagella basal body rod protein no signal peptide no TMHs; High confidence in function and specificity; flagellar basal-body rod protein FlgF 3020308 flgF 4608782 flgF Azoarcus sp. BH72 flagellar basal-body rod protein FlgF YP_934239.1 3019571 R 62928 CDS YP_934240.1 119899027 4608781 complement(3020334..3021671) 1 NC_008702.1 Flagellar hook protein flgE. InterPro: Flagella basal body rod protein no signal peptide no TMHs; High confidence in function and specificity; FlgE protein 3021671 flgE 4608781 flgE Azoarcus sp. BH72 FlgE protein YP_934240.1 3020334 R 62928 CDS YP_934241.1 119899028 4608780 complement(3021687..3022361) 1 NC_008702.1 Basal-body rod modification protein flgD. FlgD is known to be absolutely required for hook assembly, yet it has not been detected in the mature flagellum. It appears to act as a hook-capping protein to enable assembly of hook protein subunits. no signal peptide no TMHs; Family membership; basal-body rod modification protein FlgD 3022361 flgD 4608780 flgD Azoarcus sp. BH72 basal-body rod modification protein FlgD YP_934241.1 3021687 R 62928 CDS YP_934242.1 119899029 4608779 complement(3022403..3022843) 1 NC_008702.1 Flagellar basal-body rod protein flgC. The rod has been shown to consist of four different, yet evolutionary related proteins: in the distal portion of the rod there are about 26 subunits of protein flgG and in the proximal portion there are about six subunits each of proteins flgB, flgC, and flgF. These four proteins contain a highly conserved asparagine-rich domain at their N terminus. InterPro: Flagella basal body rod protein no signal peptide no TMHs; High confidence in function and specificity; flagellar basal-body rod protein FlgC 3022843 flgC 4608779 flgC Azoarcus sp. BH72 flagellar basal-body rod protein FlgC YP_934242.1 3022403 R 62928 CDS YP_934243.1 119899030 4608778 complement(3022865..3023266) 1 NC_008702.1 Flagellar basal-body rod protein flgB (Putative proximal rod protein). The rod has been shown to consist of four different, yet evolutionary related proteins: in the distal portion of the rod there are about 26 subunits of protein flgG and in the proximal portion there are about six subunits each of proteins flgB, flgC, and flgF. These four proteins contain a highly conserved asparagine-rich domain at their N terminus. no signal peptide no TMHs; High confidence in function and specificity; flagellar basal-body rod protein FlgB 3023266 flgB 4608778 flgB Azoarcus sp. BH72 flagellar basal-body rod protein FlgB YP_934243.1 3022865 R 62928 CDS YP_934244.1 119899031 4608777 3023519..3024289 1 NC_008702.1 Flagella basal body P-ring formation protein flgA precursor. Involved in the assembly process of the P-ring formation. It may associate with FlgF on the rod constituting a structure essential for the P-ring assembly or may act as a chaperon protein for the P-ring assembly (by similarity). signal peptide no TMHs; High confidence in function and specificity; chaperon for flagellar basal body P-ring formation 3024289 flgA 4608777 flgA Azoarcus sp. BH72 chaperon for flagellar basal body P-ring formation YP_934244.1 3023519 D 62928 CDS YP_934245.1 119899032 4608776 3024385..3024681 1 NC_008702.1 Region start changed from 3024385 to 3024358 (27 bases); putative negative regulator of flagellin synthesis FlgM 3024681 4608776 azo2742 Azoarcus sp. BH72 putative negative regulator of flagellin synthesis FlgM YP_934245.1 3024385 D 62928 CDS YP_934246.1 119899033 4607593 3024695..3025174 1 NC_008702.1 Putative chaperon of flagella synthesis. FlgN is an export chaperon of FlgM and FlgK involved in flagellar synthesis. no signal peptide no TMHs; Family membership; putative flagellar synthesis chaperone 3025174 flgN 4607593 flgN Azoarcus sp. BH72 putative flagellar synthesis chaperone YP_934246.1 3024695 D 62928 CDS YP_934247.1 119899034 4608788 complement(3025253..3025792) 1 NC_008702.1 Hypothetical secreted protein. Homology to cv0285 of C. violaceum of 25% (trembl|Q7P1C8) no domains predicted signal peptide TMH in signal peptide; hypothetical protein 3025792 4608788 azo2744 Azoarcus sp. BH72 hypothetical protein YP_934247.1 3025253 R 62928 CDS YP_934248.1 119899035 4607594 3025977..3026735 1 NC_008702.1 Probable DNA-binding response regulator, ITS CARBOXYL-TERMINAL MOIETY MEDIATES THE MULTIMERIZATION OF THE OMPR PROTEIN. AS A MULTIMER IT TURNS ON THE EXPRESSION OF THE OMPC GENE; AS A MONOMER IT TURNS ON THE EXPRESSION OF THE OMPF GENE.; High confidence in function and specificity; DNA-binding response regulator 3026735 ompR2 4607594 ompR2 Azoarcus sp. BH72 DNA-binding response regulator YP_934248.1 3025977 D 62928 CDS YP_934249.1 119899036 4609447 3026732..3028066 1 NC_008702.1 Putative sensor histidine kinase,; High confidence in function and specificity; putative sensor histidine kinase 3028066 envZ2 4609447 envZ2 Azoarcus sp. BH72 putative sensor histidine kinase YP_934249.1 3026732 D 62928 CDS YP_934250.1 119899037 4608672 complement(3028173..3028706) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA6852 of Azoarcus sp. EbN1 of 47% (gnl|keqq|eba:ebA6852(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3028706 4608672 azo2747 Azoarcus sp. BH72 hypothetical protein YP_934250.1 3028173 R 62928 CDS YP_934251.1 119899038 4607595 3029233..3031296 1 NC_008702.1 Putative sensory box histidine kinase, very low similarity to TREMBL: trembl|Q9FD00 (12% Erwinia stewartii, HrpX) InterPro: IPR005467 His_kinase. IPR003661 His_kinA_N. IPR000014 PAS_domain. IPR001610 PAC Pfam: PF02518 HATPase_c. PF00989 PAS. PF00785 PAC. TIGRFAM:TIGR00229 PAS domain S-box.; Function unclear; putative sensory box histidine kinase 3031296 4607595 azo2748 Azoarcus sp. BH72 putative sensory box histidine kinase YP_934251.1 3029233 D 62928 CDS YP_934252.1 119899039 4607596 3031290..3031919 1 NC_008702.1 Probable transcriptional regulator, LuxR family,; Specificity unclear; LuxR family transcriptional regulator 3031919 vsrD2 4607596 vsrD2 Azoarcus sp. BH72 LuxR family transcriptional regulator YP_934252.1 3031290 D 62928 CDS YP_934253.1 119899040 4610099 complement(3031885..3033474) 1 NC_008702.1 Putative glycerol-3-phosphate dehydrogenase. Homology to glpA of B. hermsir of 38% (trembl|Q84I21) FAD-dependent glycerol-3-phosphate dehydrogenase catalyzes the conversion of glycerol-3-phosphate into dihydroxyacetone phosphate: sn-glycerol-3-phosphate + acceptor = glycerone phosphate + reduced acceptor. InterPro: D-amino acid oxidase (IPR000927) Pfam: FAD dependent oxidoreductase no signal peptide no TMHs gidA: glucose-inhibited division prot; Family membership; putative glycerol-3-phosphate dehydrogenase 3033474 glpA 4610099 glpA Azoarcus sp. BH72 putative glycerol-3-phosphate dehydrogenase YP_934253.1 3031885 R 62928 CDS YP_934254.1 119899041 4608911 complement(3033471..3034961) 1 NC_008702.1 Probable glycerol kinase. Homology to glpK of T. aquaticus of 53% (sprot|GLPK_THEAQ). Key enzyme in the regulation of glycerol uptake and metabolism. InterPro: Carbohydrate kinase FGGY family (IPR000577) Pfam: FGGY family of carobhydrates kinases, N-terminus; FGGY family of carobhydrates kinases, C-terminus no signal peptide no TMHs; High confidence in function and specificity; glycerol kinase 3034961 glpK 4608911 glpK Azoarcus sp. BH72 glycerol kinase YP_934254.1 3033471 R 62928 CDS YP_934255.1 119899042 4608912 3035084..3035953 1 NC_008702.1 Conserved hypothetical membrane protein. Homology with CV1535 of C. violaceum of 46%. 2A78: Carboxylate/Amino Acid/Amine Tranporter. Pfam: Intergral membrane protein DUF6. probable 9 TMHs. signal peptide; Conserved hypothetical protein; hypothetical protein 3035953 4608912 azo2752 Azoarcus sp. BH72 hypothetical protein YP_934255.1 3035084 D 62928 CDS YP_934256.1 119899043 4607597 3036044..3036406 1 NC_008702.1 Conserved hypothetical protein. Homology to NE1501 of N.europaea of 47% (trembl:Q82UI4). No domains predicted. Signal peptide present. No TMH reported present.; hypothetical protein 3036406 4607597 azo2753 Azoarcus sp. BH72 hypothetical protein YP_934256.1 3036044 D 62928 CDS YP_934257.1 119899044 4607598 3036403..3037257 1 NC_008702.1 SseA: putative thiosulfate sulfurtransferase (rhodanese-like protein) [EC:2.8.1.1]. This protein transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity (130-fold lower). Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity. Probable 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2) (Rhodanese- like protein) (MST). InterPro: Rhodanese/cdc25 fold; High confidence in function and specificity; putative thiosulfate sulfurtransferase 3037257 sseA 4607598 sseA Azoarcus sp. BH72 putative thiosulfate sulfurtransferase YP_934257.1 3036403 D 62928 CDS YP_934258.1 119899045 4609893 complement(3037292..3038107) 1 NC_008702.1 converts L-glutamate to D-glutamate, a component of peptidoglycan; glutamate racemase 3038107 murI 4609893 murI Azoarcus sp. BH72 glutamate racemase YP_934258.1 3037292 R 62928 CDS YP_934259.1 119899046 4609294 complement(3038104..3039303) 1 NC_008702.1 catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr); tyrosyl-tRNA synthetase 3039303 4609294 azo2756 Azoarcus sp. BH72 tyrosyl-tRNA synthetase YP_934259.1 3038104 R 62928 CDS YP_934260.1 119899047 4607599 3039381..3040709 1 NC_008702.1 Hypothetical metalloprotease yebA precursor (EC 3.4.24.-).; Specificity unclear; metalloprotease 3040709 4607599 azo2757 Azoarcus sp. BH72 metalloprotease YP_934260.1 3039381 D 62928 CDS YP_934261.1 119899048 4607600 complement(3040778..3041128) 1 NC_008702.1 essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors; iron-sulfur cluster insertion protein ErpA 3041128 4607600 azo2758 Azoarcus sp. BH72 iron-sulfur cluster insertion protein ErpA YP_934261.1 3040778 R 62928 CDS YP_934262.1 119899049 4607601 complement(3041369..3041761) 1 NC_008702.1 forms a direct contact with the tRNA during translation; 30S ribosomal protein S9 3041761 rpsI 4607601 rpsI Azoarcus sp. BH72 30S ribosomal protein S9 YP_934262.1 3041369 R 62928 CDS YP_934263.1 119899050 4609812 complement(3041775..3042203) 1 NC_008702.1 in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit; 50S ribosomal protein L13 3042203 rplM 4609812 rplM Azoarcus sp. BH72 50S ribosomal protein L13 YP_934263.1 3041775 R 62928 CDS YP_934264.1 119899051 4609772 3042732..3043124 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc0489 of R. solanacearum of 73% (trembl|Q8Y247). no signal peptide. no TMH. No domains predicted.; Family membership; hypothetical protein 3043124 4609772 azo2761 Azoarcus sp. BH72 hypothetical protein YP_934264.1 3042732 D 62928 CDS YP_934265.1 119899052 4607602 complement(3043258..3043878) 1 NC_008702.1 Probable Ubiquinone biosynthesis protein COQ7 homolog (Coenzyme Q biosynthesis protein 7 homolog) (Timing protein clk-1 homolog) (Fragment).; Function unclear; ubiquinone biosynthesis protein 3043878 4607602 azo2762 Azoarcus sp. BH72 ubiquinone biosynthesis protein YP_934265.1 3043258 R 62928 CDS YP_934266.1 119899053 4607603 complement(3043881..3044411) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA945 of Azoarcus sp. EbN1 of 47% (gnl|keqq|eba:ebA945(KEGG)). No domains predicted. Signal P reporting signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3044411 4607603 azo2763 Azoarcus sp. BH72 hypothetical protein YP_934266.1 3043881 R 62928 CDS YP_934267.1 119899054 4607604 complement(3044471..3044998) 1 NC_008702.1 hydrolyzes diadenosine polyphosphate; dinucleoside polyphosphate hydrolase 3044998 4607604 azo2764 Azoarcus sp. BH72 dinucleoside polyphosphate hydrolase YP_934267.1 3044471 R 62928 CDS YP_934268.1 119899055 4607605 3045152..3046897 1 NC_008702.1 catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro); prolyl-tRNA synthetase 3046897 4607605 azo2765 Azoarcus sp. BH72 prolyl-tRNA synthetase YP_934268.1 3045152 D 62928 CDS YP_934269.1 119899056 4607606 3046921..3047592 1 NC_008702.1 Probable Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-) (Murein hydrolase C).; Function unclear; soluble lytic murein transglycosylase 3047592 4607606 azo2766 Azoarcus sp. BH72 soluble lytic murein transglycosylase YP_934269.1 3046921 D 62928 CDS YP_934270.1 119899057 4607607 complement(3047582..3048178) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03000304 of Dechloromonas aromatica of 54% (gi|46140561|ref|ZP_00152132.2|(NBCI ENTREZ)). Has PF04519; Protein of unknown function, DUF583:This family contains several uncharacterised hypothetical proteins. No Signal peptide or TMH reported as present.; hypothetical protein 3048178 4607607 azo2767 Azoarcus sp. BH72 hypothetical protein YP_934270.1 3047582 R 62928 CDS YP_934271.1 119899058 4607608 3048521..3049915 1 NC_008702.1 Putative two-component system sensor kinase,; Family membership; putative two-component system sensor kinase 3049915 yfhK 4607608 yfhK Azoarcus sp. BH72 putative two-component system sensor kinase YP_934271.1 3048521 D 62928 CDS YP_934272.1 119899059 4610174 3049908..3050480 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA964 of Azoarcus sp. EbN1 of 33% (gnl|keqq|eba:ebA964(KEGG)). No domains predicted. Signal P reporting signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 3050480 4610174 azo2769 Azoarcus sp. BH72 hypothetical protein YP_934272.1 3049908 D 62928 CDS YP_934273.1 119899060 4607609 3050477..3051874 1 NC_008702.1 Putative two component transcriptional regulator,; Conserved hypothetical protein; putative two component transcriptional regulator 3051874 yfhA 4607609 yfhA Azoarcus sp. BH72 putative two component transcriptional regulator YP_934273.1 3050477 D 62928 CDS YP_934274.1 119899061 4610172 3051907..3053010 1 NC_008702.1 hypothetical protein 3053010 4610172 azo2771 Azoarcus sp. BH72 hypothetical protein YP_934274.1 3051907 D 62928 CDS YP_934275.1 119899062 4607610 complement(3053030..3054127) 1 NC_008702.1 catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; tRNA-specific 2-thiouridylase MnmA 3054127 mnmA 4607610 mnmA Azoarcus sp. BH72 tRNA-specific 2-thiouridylase MnmA YP_934275.1 3053030 R 62928 CDS YP_934276.1 119899063 4607611 complement(3054144..3054806) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA988 of Azoarcus sp. EbN1 of 43% (gnl|keqq|eba:ebA988(KEGG)). no domains predicted. signal peptie. no TMHs; Conserved hypothetical protein; hypothetical protein 3054806 4607611 azo2773 Azoarcus sp. BH72 hypothetical protein YP_934276.1 3054144 R 62928 CDS YP_934277.1 119899064 4607612 complement(3054806..3055288) 1 NC_008702.1 Putative Nudix hydrolase ymfB (EC 3.6.-.-). mutt: mutator mutT protein; Specificity unclear; hypothetical protein 3055288 4607612 azo2774 Azoarcus sp. BH72 hypothetical protein YP_934277.1 3054806 R 62928 CDS YP_934278.1 119899065 4607613 3055412..3056332 1 NC_008702.1 catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids; branched-chain amino acid aminotransferase 3056332 ilvE2 4607613 ilvE2 Azoarcus sp. BH72 branched-chain amino acid aminotransferase YP_934278.1 3055412 D 62928 CDS YP_934279.1 119899066 4609073 3056343..3056543 1 NC_008702.1 Hypothetical protein , 47% identity to TrEMBL;Q7NW97,TrEMBL;Q7VTV4(56% identity). No domains,repeats, motifs or features present.; Function unclear; hypothetical protein 3056543 4609073 azo2776 Azoarcus sp. BH72 hypothetical protein YP_934279.1 3056343 D 62928 CDS YP_934280.1 119899067 4607614 3056655..3057674 1 NC_008702.1 Conserved hypothetical protein. Homology to ne1289 of N. europaea of 49% (trembl|Q82V21). InterPro: Esterase/lipase/thioesterase family active site (IPR000379). no signal peptide. no TMH.; hypothetical protein 3057674 4607614 azo2777 Azoarcus sp. BH72 hypothetical protein YP_934280.1 3056655 D 62928 CDS YP_934281.1 119899068 4607615 3057734..3059125 1 NC_008702.1 Phosphomannomutase/phosphoglucomutase (PMM / PGM). The phosphomannomutase activity produces a precursor for alginate polymerization. This enzyme participates in both the breakdown and synthesis of glucose.Catalytic activity: Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate. 58% PG/PMM_mutase.IPR005844; PG_PMM_ABAI.IPR005845; PG_PMM_ABAII.IPR005846; PG_PMM_ABAIII.IPR005843; PG_PMM_C. Pfam:PF02878; PGM_PMM_I; 1.PF02879; PGM_PMM_II; 1.PF02880; PGM_PMM_III. PF00408; PGM_PMM_IV; 1.; High confidence in function and specificity; putative phosphoglucomutase 3059125 pgm 4607615 pgm Azoarcus sp. BH72 putative phosphoglucomutase YP_934281.1 3057734 D 62928 CDS YP_934282.1 119899069 4609532 3059222..3060082 1 NC_008702.1 Conserved hypothetical protein with unknown function. No domains predicted. Similarity to TREMBL: trembl|Q9I6D7 (33% Pseudomonas aeruginosa, hypothetical protein pa0356) / trembl|Q88CQ4 (30% Pseudomonas putida (strain KT2440), pp5126); hypothetical protein 3060082 4609532 azo2779 Azoarcus sp. BH72 hypothetical protein YP_934282.1 3059222 D 62928 CDS YP_934283.1 119899070 4607616 complement(3060174..3062831) 1 NC_008702.1 Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; alanyl-tRNA synthetase 3062831 alaS 4607616 alaS Azoarcus sp. BH72 alanyl-tRNA synthetase YP_934283.1 3060174 R 62928 CDS YP_934284.1 119899071 4608291 3062977..3063528 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA1022 of Azoarcus sp. EbN1 of 86% (gnl|keqq|eba:ebA1022(KEGG)). Has PF01923, Cobalamin adenosyltransferase;IPR002779,DUF80; This family contains the gene products of PduO and EutT which are both cobalamin adenosyltransferases. PduO is a protein with ATP:cob(I)alamin adenosyltransferase activity. The main role of this protein is the conversion of inactive cobalamins to AdoCbl for 1,2-propanediol degradation. The EutT enzyme appears to be an adenosyl transferase, converting CNB12 to AdoB12. No signal peptide or TMH present.; hypothetical protein 3063528 4608291 azo2781 Azoarcus sp. BH72 hypothetical protein YP_934284.1 3062977 D 62928 CDS YP_934285.1 119899072 4607617 complement(3063558..3063926) 1 NC_008702.1 Conserved hypothetical protein. Homology to am orf of Azotobacter vinelandii of 51% (gi|67087339|gb|EAM06806.1). Pfam: Dinitrogenase iron-molybdenum cofactor. This family contains several NIF (B,Y and X) proteins which are iron-molybdenum cofactors (FeMo-co) in the dinitrogenase enzyme which catalyses the reduction of dinitrogen to ammonium. No signal peptide. No TMHs.; hypothetical protein 3063926 4607617 azo2782 Azoarcus sp. BH72 hypothetical protein YP_934285.1 3063558 R 62928 CDS YP_934286.1 119899073 4607618 3063993..3064577 1 NC_008702.1 Probable RNA-directed RNA polymerase (EC 2.7.7.48) (RNA replicase) (216.5 kDa protein) (ORF1). alkb: alkylated DNA repair protein AlkB.; Function unclear; DNA repair system specific for alkylated DNA 3064577 4607618 azo2783 Azoarcus sp. BH72 DNA repair system specific for alkylated DNA YP_934286.1 3063993 D 62928 CDS YP_934287.1 119899074 4607619 3064764..3065855 1 NC_008702.1 Conserved hypothetical monoamine oxidase. Homology to rsp0431 of R. solanacearum of 34% (trembl|Q8XSN6). In lower eukaryotes such as aspergillus and in bacteria the main role of amine oxidases is to provide a source of ammonium. InterPro: Amine oxidase (IPR002937). Pfam: Flavin containing amine oxidase. probable signal peptide. no TMH.; Family membership; monoamine oxidase 3065855 4607619 azo2784 Azoarcus sp. BH72 monoamine oxidase YP_934287.1 3064764 D 62928 CDS YP_934288.1 119899075 4607620 complement(3065910..3067784) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to SC3D11.05C of Streptomyces coelicolor of 33% (trembl|Q9L1F7(SRS)). Pfam (PF00005): ABC transporter. Pfam (PF00664): ABC transporter transmembrane region. TMHMM reporting three transmembrane helices. No signal peptide.; Conserved hypothetical protein; hypothetical protein 3067784 4607620 azo2785 Azoarcus sp. BH72 hypothetical protein YP_934288.1 3065910 R 62928 CDS YP_934289.1 119899076 4607621 complement(3067852..3068925) 1 NC_008702.1 involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function; DNA polymerase IV 3068925 dinP 4607621 dinP Azoarcus sp. BH72 DNA polymerase IV YP_934289.1 3067852 R 62928 CDS YP_934290.1 119899077 4608621 complement(3068980..3070395) 1 NC_008702.1 Conserved hypothetical RumC homolog. Homology to rumC of P.aeruginosa of 43% (gi|20139744|sp|Q9I4U3|RMUC_PSEAE). Pfam: RmuC family. This family contains several bacterial RmuC DNA recombination proteins. The function of the RMUC protein is unknown but it is suspected that it is either a structural protein that protects DNA against nuclease action, or is itself involved in DNA cleavage at the regions of DNA secondary structures. Signal peptide predicted. 2 TMHs.,; Conserved hypothetical protein; putative RumC-like protein 3070395 rumC 4608621 rumC Azoarcus sp. BH72 putative RumC-like protein YP_934290.1 3068980 R 62928 CDS YP_934291.1 119899078 4609834 complement(3070509..3071594) 1 NC_008702.1 catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase 3071594 aroG 4609834 aroG Azoarcus sp. BH72 phospho-2-dehydro-3-deoxyheptonate aldolase YP_934291.1 3070509 R 62928 CDS YP_934292.1 119899079 4608347 3071733..3072989 1 NC_008702.1 Glutathione-regulated potassium-efflux system protein kefC (K(+)/H(+) antiporter). Transport system that facilitates potassium-efflux possibly by potassium-proton antiport.Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KefC subfamily. 23% K_eff.IPR006153; Na_H_porter. Pfam:PF00999; Na_H_Exchanger; 1. TIGRFAMs:TIGR00932; 2a37; 1. TMHelix:11; High confidence in function and specificity; putative cation/proton antiporter 3072989 kefC 4608347 kefC Azoarcus sp. BH72 putative cation/proton antiporter YP_934292.1 3071733 D 62928 CDS YP_934293.1 119899080 4609106 3073104..3074060 1 NC_008702.1 in Escherichia coli this periplasmic enzyme was found to encode the periplasmic catalytic subunit of an oxidoreductase; sulfite oxidase activity not demonstrated; requires inner membrane anchor protein YedZ; putative sulfite oxidase subunit YedY 3074060 4609106 azo2790 Azoarcus sp. BH72 putative sulfite oxidase subunit YedY YP_934293.1 3073104 D 62928 CDS YP_934294.1 119899081 4607622 3074075..3074752 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv0790 of C. violaceum of 50% (tremble:Q7NZX9). Has PF05252, Uncharacterised protein family (UPF0191);IPR007916 ; This family of proteins is functionally uncharacterised. signal peptide. 5 TMHs; Conserved hypothetical protein; hypothetical protein 3074752 4607622 azo2791 Azoarcus sp. BH72 hypothetical protein YP_934294.1 3074075 D 62928 CDS YP_934295.1 119899082 4607623 complement(3074783..3075376) 1 NC_008702.1 forms a trimer; related to eukaryotic protein gephyrin; functions during molybdenum cofactor biosynthesis; molybdenum cofactor biosynthesis protein MogA 3075376 mogA 4607623 mogA Azoarcus sp. BH72 molybdenum cofactor biosynthesis protein MogA YP_934295.1 3074783 R 62928 CDS YP_934296.1 119899083 4609259 complement(3075373..3075936) 1 NC_008702.1 Hypothetical protein yjgA, 46% identity(59% similarity) to SwissProt;Q8FAF3.SwissProt;Q83P53 Has PF04751, Protein of unknown function (DUF615);IPR006839;This family of bacterial proteins has no known function. NO Signal Peptide or TMH reported present.; Function unclear; hypothetical protein 3075936 yjgA 4609259 yjgA Azoarcus sp. BH72 hypothetical protein YP_934296.1 3075373 R 62928 CDS YP_934297.1 119899084 4610189 3076001..3077341 1 NC_008702.1 Probable PmbA protein. Homology to pmbA of E. coli of 48% (sprot|PMBA_ECOLI). MAY FACILITATE THE SECRETION OF THE PEPTIDE ANTIBIOTIC MICROCIN B17 (MCCB17) BY COMPLETING ITS MATURATION. SUPPRESSES THE INHIBITORY ACTIVITY OF THE CARBON STORAGE REGULATOR (CSRA). Pfam: Putative modulator of DNA gyrase no signal peptide no TMHs; High confidence in function and specificity; PmbA protein 3077341 pmbA 4610189 pmbA Azoarcus sp. BH72 PmbA protein YP_934297.1 3076001 D 62928 CDS YP_934298.1 119899085 4609587 3077588..3078736 1 NC_008702.1 Similar to TREMBL:Q8G2H7 (58% identity); TREMBL:Q8UK55 (57% identity); TREMBL:Q89NT9 (58% identity). SignalP reporting signal peptide.; Specificity unclear; putative periplasmic substrate binding protein 3078736 4609587 azo2795 Azoarcus sp. BH72 putative periplasmic substrate binding protein YP_934298.1 3077588 D 62928 CDS YP_934299.1 119899086 4607624 complement(3078912..3080594) 1 NC_008702.1 Similar to TREMBL:Q7WQP2 (61% identity); TREMBL:Q7W1R6 (61% identity); TREMBL:Q8G2H8 (50% identity). TIGRFAM (TIGR00786): TRAP transporter DctM subunit. TMHMM predicting 14 transmembrane helices.; Specificity unclear; putative large permease of TRAP-type transporter 3080594 4607624 azo2796 Azoarcus sp. BH72 putative large permease of TRAP-type transporter YP_934299.1 3078912 R 62928 CDS YP_934300.1 119899087 4607625 complement(3080591..3081184) 1 NC_008702.1 Similar to TREMBL:Q7WQP3 52% identity); TREMBL:Q92P73 (54% identity); TREMBL:Q8UK53 (48% identity). TMHMM reporting four transmembrane helices.; Specificity unclear; putative small permease of TRAP-type transporter 3081184 4607625 azo2797 Azoarcus sp. BH72 putative small permease of TRAP-type transporter YP_934300.1 3080591 R 62928 CDS YP_934301.1 119899088 4607626 3081308..3081982 1 NC_008702.1 Probable phosphoglycerate mutase GpmB (phosphoglyceromutase) 0(Pgam). Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate 31% PG/BPGM_mutase. Pfam:PF00300; PGAM; 1.; High confidence in function and specificity; phosphoglycerate mutase 3081982 gpmB 4607626 gpmB Azoarcus sp. BH72 phosphoglycerate mutase YP_934301.1 3081308 D 62928 CDS YP_934302.1 119899089 4608933 3082083..3083501 1 NC_008702.1 Probable two component sensor protein,; High confidence in function and specificity; two component sensor protein 3083501 kdpD1 4608933 kdpD1 Azoarcus sp. BH72 two component sensor protein YP_934302.1 3082083 D 62928 CDS YP_934303.1 119899090 4609100 3083510..3084190 1 NC_008702.1 Transcriptional regulatory protein KdpE,; High confidence in function and specificity; two component system transcriptional regulatory protein 3084190 kdpE 4609100 kdpE Azoarcus sp. BH72 two component system transcriptional regulatory protein YP_934303.1 3083510 D 62928 CDS YP_934304.1 119899091 4609102 3084401..3085651 1 NC_008702.1 catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase 3085651 glyA 4609102 glyA Azoarcus sp. BH72 serine hydroxymethyltransferase YP_934304.1 3084401 D 62928 CDS YP_934305.1 119899092 4608924 3085693..3086208 1 NC_008702.1 Transcriptional regulator,; Conserved hypothetical protein; transcriptional regulator NrdR 3086208 nrdR 4608924 nrdR Azoarcus sp. BH72 transcriptional regulator NrdR YP_934305.1 3085693 D 62928 CDS YP_934306.1 119899093 4609726 3086189..3087280 1 NC_008702.1 Riboflavin biosynthesis protein ribD [Includes: Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) (Riboflavin-specific deaminase); 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) (HTP reductase)]. CONVERTS 25-DIAMINO-6-(RIBOSYLAMINO)-4(3H)-PYRIMIDINONE 5-PHOSPHATE INTO 5-AMINO-6-(RIBOSYLAMINO)-24(1H3H)- PYRIMIDINEDIONE 5-PHOSPHATE. InterPro: Riboflavin biosynthesis protein RibD TIGRFAM: eubact_ribD: riboflavin biosynthesis protein; High confidence in function and specificity; AMP deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase 3087280 ribD 4609726 ribD Azoarcus sp. BH72 AMP deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase YP_934306.1 3086189 D 62928 CDS YP_934307.1 119899094 4609722 complement(3087294..3088043) 1 NC_008702.1 Dinucleotide-utilizing enzyme involved in molybdopterin and/or thiamine biosynthesis Belongs to the hesA/moeB/thiF family, by similarity. TIGR00292: thiazole biosynthesis enzyme; High confidence in function and specificity; adenylyltransferase 3088043 4609722 azo2804 Azoarcus sp. BH72 adenylyltransferase YP_934307.1 3087294 R 62928 CDS YP_934308.1 119899095 4607627 complement(3088140..3088598) 1 NC_008702.1 Conserved hypothetical protein. Homlogy to ebA1048 Azoarcus sp. EbN1 of 65% (gnl|keqq|eba:ebA1048(KEGG)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3088598 4607627 azo2805 Azoarcus sp. BH72 hypothetical protein YP_934308.1 3088140 R 62928 CDS YP_934309.1 119899096 4607628 complement(3088619..3089998) 1 NC_008702.1 Carboxy-terminal processing protease precursor (EC 3.4.21.102) (C- terminal processing protease). prc: carboxyl-terminal protease; High confidence in function and specificity; carboxy-terminal processing protease 3089998 ctpa 4607628 ctpa Azoarcus sp. BH72 carboxy-terminal processing protease YP_934309.1 3088619 R 62928 CDS YP_934310.1 119899097 4608541 complement(3090060..3091394) 1 NC_008702.1 Homology to peptidase, M23/M37 family InterPro: Peptidase family M23/M37; hypothetical protein 3091394 4608541 azo2807 Azoarcus sp. BH72 hypothetical protein YP_934310.1 3090060 R 62928 CDS YP_934311.1 119899098 4607629 complement(3091417..3092166) 1 NC_008702.1 2,3-bisphosphoglycerate-dependent; catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate; phosphoglyceromutase 3092166 gpmA 4607629 gpmA Azoarcus sp. BH72 phosphoglyceromutase YP_934311.1 3091417 R 62928 CDS YP_934312.1 119899099 4608932 3092165..3092641 1 NC_008702.1 Putative transcriptional activator, ArsR family,; High confidence in function and specificity; ArsR family transcriptional regulator 3092641 hlyU 4608932 hlyU Azoarcus sp. BH72 ArsR family transcriptional regulator YP_934312.1 3092165 D 62928 CDS YP_934313.1 119899100 4609011 3092677..3093093 1 NC_008702.1 Similar to Rhodanese domain protein, a sulphurtransferase involved in cyanide detoxification. Hypothetical protein slr1261. InterPro: Rhodanese/cdc25 fold; Function unclear; hypothetical protein 3093093 4609011 azo2810 Azoarcus sp. BH72 hypothetical protein YP_934313.1 3092677 D 62928 CDS YP_934314.1 119899101 4607630 3093090..3093362 1 NC_008702.1 Probable glutaredoxin. Homology to grx3 of E. coli. of 53% (SWISSPROT:GLR3_ECOLI). The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing some disulfides in a coupled system with glutathione reductase. Pfam: Glutaredoxin Tigrfam: TIGR00365: glutaredoxin-related protein no signal peptide no TMHs; High confidence in function and specificity; glutaredoxin 3093362 grxC 4607630 grxC Azoarcus sp. BH72 glutaredoxin YP_934314.1 3093090 D 62928 CDS YP_934315.1 119899102 4608943 3093432..3093887 1 NC_008702.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 3093887 secB 4608943 secB Azoarcus sp. BH72 preprotein translocase subunit SecB YP_934315.1 3093432 D 62928 CDS YP_934316.1 119899103 4609858 3093892..3094338 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV1128 of Chromobacterium violaceum of 43% (trembl:Q7NYZ4). Has Signal Peptide. Has PF06347, Protein of unknown function (DUF1058);IPR010466 ;This family consists of several hypothetical bacterial proteins of unknown function. No TMHs; Conserved hypothetical protein; hypothetical protein 3094338 4609858 azo2813 Azoarcus sp. BH72 hypothetical protein YP_934316.1 3093892 D 62928 CDS YP_934317.1 119899104 4607631 3094335..3095324 1 NC_008702.1 catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 3095324 gpsA 4607631 gpsA Azoarcus sp. BH72 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase YP_934317.1 3094335 D 62928 CDS YP_934318.1 119899105 4608934 complement(3095339..3095872) 1 NC_008702.1 Region start changed from 3095806 to 3095845 (39 bases); tRNA/rRNA methylase 3095872 4608934 azo2815 Azoarcus sp. BH72 tRNA/rRNA methylase YP_934318.1 3095339 R 62928 CDS YP_934319.1 119899106 4607632 complement(3095879..3096571) 1 NC_008702.1 Hypothetical protein yhgH. Members of PRT family are catalytic and regulatory proteins involved in nucleotide synthesis and salvage. The name PRT comes from phosphoribosyltransferase enzymes, which carry out phosphoryl transfer reactions on PRPP, an activated form of ribose-5-phosphate SPROT:P46846: 33% identity, 51% similarity InterPro; IPR000836; PRtransferase. Pfam; PF00156; Pribosyltran; 1 Absence of Signal peptide and Transmembrane helices PPR: pentatricopeptide repeat domain; High confidence in function and specificity; putative competence protein F 3096571 comF 4607632 comF Azoarcus sp. BH72 putative competence protein F YP_934319.1 3095879 R 62928 CDS YP_934320.1 119899107 4608511 3096650..3097630 1 NC_008702.1 Biotin synthase (Biotin synthetase). catalytic activity: dethiobiotin + sulfur = biotin. cofactor: binds a 4fe-4s cluster coordinated with 3 cysteines and an exchangeable s-adenosyl-l-methionine, and a 2fe-2s cluster coordinated with 3 cysteines and 1 arginine. pathway: biotin biosynthesis; last step. similarity: belongs to the biotin and lipoic acid synthetases family. TIGRFAM: bioB: biotin synthetase; High confidence in function and specificity; biotin synthase 3097630 bioB 4608511 bioB Azoarcus sp. BH72 biotin synthase YP_934320.1 3096650 D 62928 CDS YP_934321.1 119899108 4608390 3097630..3098547 1 NC_008702.1 FUNCTION:BioC is involved in an early, but chemically unexplored, step in the conversion of pimelic acid to biotin. PATHWAY:Biotin biosynthesis. SIMILARITY:Belongs to the methyltransferase superfamily TREMBL:Q7NPW6 - 46% identity,61% similarity to Chromobacterium bioC. TREMBL:Q82SL9 InterPro (IPR000051): SAM (and some other nucleotide) binding motif. Pfam (PF01209): ubiE/COQ5 methyltransferase family.; Family membership; putative biotin synthesis protein 3098547 bioC2 4608390 bioC2 Azoarcus sp. BH72 putative biotin synthesis protein YP_934321.1 3097630 D 62928 CDS YP_934322.1 119899109 4608392 3098555..3099211 1 NC_008702.1 TREMBL:Q7NQN9: 48% identity; 66% similarity SPROT:P53078: 28% identity, 47% similarity InterPro :IPR006402; HAD-SF-IA-v3. IPR005833: Hlgnase/hydrlase. IPR005834: Hydrolase. IPR010237; Pyr-5-nucltdase. Pfam PF00702; Hydrolase; 1. PRINTS PR00413; HADHALOGNASE. TIGRFAMs TIGR01509; HAD-SF-IA-v3; 1. TIGR01993; Pyr-5-nucltdase; SSM1 protein. No signal peptide. No transmembrane helices. Could be an enzyme that inactivates 6-azauracil by modifying it. InterPro: Haloacid dehalogenase/epoxide hydrolase family otsB: trehalose-phosphatase; Family membership; putative hydrolase 3099211 ssm 4608392 ssm Azoarcus sp. BH72 putative hydrolase YP_934322.1 3098555 D 62928 CDS YP_934323.1 119899110 4609894 complement(3099203..3099697) 1 NC_008702.1 Putative acetyltransferase,42% identity to TrEMBL;Q7WFK1, Putative acetyltransferase [BB4270] [Bordetella bronchiseptica(Alcaligenes bronchisepticus)]. Has PF00583:IPR000182;Acetyltransferase (GNAT) family;This family contains proteins with N-acetyltransferase functions.Histone acetylation is carried out by a class of enzymes known as histone acetyltransferases (HATs), which catalyze the transfer of an acetyl group from acetyl-CoA to the lysine E -amino groups on the N-terminal tails of histones. Early indication that HATs were involved in transcription came from the observation that in actively transcribed regions of chromatin, histones tend to be hyperacetylated, whereas in transcriptionally silent regions histones are hypoacetylated. The histone acetyltransferases are divided into five families.; putative acetyltransferase 3099697 4609894 azo2820 Azoarcus sp. BH72 putative acetyltransferase YP_934323.1 3099203 R 62928 CDS YP_934324.1 119899111 4607633 complement(3099739..3100743) 1 NC_008702.1 catalyzes the formation of porphobilinogen from 5-aminolevulinate; delta-aminolevulinic acid dehydratase 3100743 hemB 4607633 hemB Azoarcus sp. BH72 delta-aminolevulinic acid dehydratase YP_934324.1 3099739 R 62928 CDS YP_934325.1 119899112 4608980 complement(3100989..3101618) 1 NC_008702.1 binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential; ribosome biogenesis GTP-binding protein YsxC 3101618 engB 4608980 engB Azoarcus sp. BH72 ribosome biogenesis GTP-binding protein YsxC YP_934325.1 3100989 R 62928 CDS YP_934326.1 119899113 4608669 3101743..3102369 1 NC_008702.1 Probable cytochrome c4. Homology to cc4 of A. vinelandii of 42% (sprot|CYC4_AZOVI). Diheme, high potential cytochrome c believed to be an intermediate electron donor to terminal oxidation systems. Pfam: cytochrome C signal peptide no TMHs; High confidence in function and specificity; cytochrome C4 3102369 cc42 4608669 cc42 Azoarcus sp. BH72 cytochrome C4 YP_934326.1 3101743 D 62928 CDS YP_934327.1 119899114 4608439 complement(3102434..3103303) 1 NC_008702.1 Hypothetical protein, 51% identity (59% similarity) to TrEMBL;Q8Y0B0. TrEMBL; Q7NWN1(53% identity). Has PF04305, Protein of unknown function (DUF455).IPR007402. Has no signal Peptide or TMH present.; Function unclear; hypothetical protein 3103303 4608439 azo2824 Azoarcus sp. BH72 hypothetical protein YP_934327.1 3102434 R 62928 CDS YP_934328.1 119899115 4607634 3103351..3104595 1 NC_008702.1 Molybdopterin biosynthesis protein moeA. Involved in the biosynthesis of a demolybdo-cofactor (molybdopterin) necessary for molybdo-enzymes. Seems to be involved in a step of activation of molybdenum in the form of thiomolybdenum. InterPro: Molybdenum cofactor biosynthesis protein molyb_syn: molybdenum cofactor synthesis; High confidence in function and specificity; molybdopterin biosynthesis protein 3104595 moeA2 4607634 moeA2 Azoarcus sp. BH72 molybdopterin biosynthesis protein YP_934328.1 3103351 D 62928 CDS YP_934329.1 119899116 4609258 3104643..3105311 1 NC_008702.1 Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway; ribose-5-phosphate isomerase A 3105311 rpiA 4609258 rpiA Azoarcus sp. BH72 ribose-5-phosphate isomerase A YP_934329.1 3104643 D 62928 CDS YP_934330.1 119899117 4609761 3105356..3106069 1 NC_008702.1 Phosphate transport system protein phoU. Probably involved in phosphate transport and metabolism. In P aeruginosa PhoU is involved in the regulation of alkaline phosphatase expression and in the induction of Pi taxis.; High confidence in function and specificity; phosphate uptake regulator 3106069 phoU 4609761 phoU Azoarcus sp. BH72 phosphate uptake regulator YP_934330.1 3105356 D 62928 CDS YP_934331.1 119899118 4609552 complement(3106151..3106423) 1 NC_008702.1 Hpyothetical Protein, 69% identity (84% simialarity) to SwissProt; Q8Y010. Has PF04362, Protein of unknown function (DUF495);IPR007457;Methionine start codon is known to be cleaved from Escherichia coli protein YggX (P52065). YggX is also known to be highly abundant in E. coli K-12. No Signal peptide or TMH present.; hypothetical protein 3106423 4609552 azo2828 Azoarcus sp. BH72 hypothetical protein YP_934331.1 3106151 R 62928 CDS YP_934332.1 119899119 4607635 complement(3106427..3108166) 1 NC_008702.1 GGDEF/PAS/PAC-domain containing protein; diguanylate cyclase 3108166 4607635 azo2829 Azoarcus sp. BH72 diguanylate cyclase YP_934332.1 3106427 R 62928 CDS YP_934333.1 119899120 4607636 complement(3108245..3109603) 1 NC_008702.1 catalyzes the formation of N-acetyl-L-glutamate from L-glutamate and acetyl-CoA in arginine biosynthesis; N-acetylglutamate synthase 3109603 argA 4607636 argA Azoarcus sp. BH72 N-acetylglutamate synthase YP_934333.1 3108245 R 62928 CDS YP_934334.1 119899121 4608331 complement(3109740..3111248) 1 NC_008702.1 Hypothetical protein, 53% identity (69% similarity) to TrEMBL;Q7NQR2. TREMBL;Q74F81. Has 2 copies of PF03976,IPR005660, Domain of unknown function (DUF344);This presumed domain is found in one or two copies per protein. The domain is about 230 amino acids in length and has many conserved motifs that are probably functionally important. No Signal Peptide or TMH present.; hypothetical protein 3111248 4608331 azo2831 Azoarcus sp. BH72 hypothetical protein YP_934334.1 3109740 R 62928 CDS YP_934335.1 119899122 4607637 complement(3111292..3112023) 1 NC_008702.1 in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase; 16S ribosomal RNA methyltransferase RsmE 3112023 4607637 azo2832 Azoarcus sp. BH72 16S ribosomal RNA methyltransferase RsmE YP_934335.1 3111292 R 62928 CDS YP_934336.1 119899123 4607638 3112072..3112881 1 NC_008702.1 Inositol-1-monophosphatase(IMPase)(Inositol-1- phosphatase)(I-1-Pase).May act by enhancing the synthesis or degradation of phosphorylated messenger molecules. Catalytic activity: myo-inositol 1-phosphate + h(2)o = myo- inositol + phosphate. 36% Inositol_P. Pfam:PF00459; Inositol_P; 1.; High confidence in function and specificity; putative inositol-1-monophosphatase 3112881 suhB2 4607638 suhB2 Azoarcus sp. BH72 putative inositol-1-monophosphatase YP_934336.1 3112072 D 62928 CDS YP_934337.1 119899124 4609907 3112954..3113214 1 NC_008702.1 Hypothetical protein, has very weak homology with hits in the database. Has No domains, repeats, motifs or features could be predicted with confidence above threshold scores.; hypothetical protein 3113214 4609907 azo2834 Azoarcus sp. BH72 hypothetical protein YP_934337.1 3112954 D 62928 CDS YP_934338.1 119899125 4607639 3113267..3114136 1 NC_008702.1 Probable phosphoribulokinase. Homology to prkB of r. sphaeroides of 59% (sprot|KPP2_RHOSH). Phosphoribulokinase catalyses the ATP-dependent phosphorylation of ribulose-5-phosphate to ribulose-1,5-phosphate, a key step in the pentose phosphate pathway where carbon dioxide is assimilated by autotrophic organisms. InterPro: Phosphoribulokinase family (IPR006082) Pfam: Phosphoribulokinase no signal peptide no TMHs; High confidence in function and specificity; phosphoribulokinase 3114136 prkB 4607639 prkB Azoarcus sp. BH72 phosphoribulokinase YP_934338.1 3113267 D 62928 CDS YP_934339.1 119899126 4609629 3114267..3116330 1 NC_008702.1 catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase 3116330 tkt 4609629 tkt Azoarcus sp. BH72 transketolase YP_934339.1 3114267 D 62928 CDS YP_934340.1 119899127 4609950 3116428..3117444 1 NC_008702.1 Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)plays an important role in glycolysis and gluconeogenesis by reversibly catalysing the oxidation and phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphospho- glycerate.61% GAPDH-I.IPR000173; GAP_dhdrogenase. Pfam:PF02800; Gp_dh_C; 1.PF00044; Gp_dh_N; 1. TIGRFAMs:TIGR01534; GAPDH-I; 1.; High confidence in function and specificity; putative glyceraldehyde 3-phosphate dehydrogenase 3117444 gapA 4609950 gapA Azoarcus sp. BH72 putative glyceraldehyde 3-phosphate dehydrogenase YP_934340.1 3116428 D 62928 CDS YP_934341.1 119899128 4608858 3117462..3117929 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to xcc3191 of X. campestris of 41% (trembl|Q8P5Z1). no domains predicted. signal peptide. no TMHs.; Function unclear; hypothetical protein 3117929 4608858 azo2838 Azoarcus sp. BH72 hypothetical protein YP_934341.1 3117462 D 62928 CDS YP_934342.1 119899129 4607640 3118003..3119187 1 NC_008702.1 Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway; phosphoglycerate kinase 3119187 pgk 4607640 pgk Azoarcus sp. BH72 phosphoglycerate kinase YP_934342.1 3118003 D 62928 CDS YP_934343.1 119899130 4609531 complement(3119266..3119676) 1 NC_008702.1 Hypothetical secreted protein. No good homology to the data bank. No domains predicted. Signal peptide reported to be present. No TMH reported present. ssl1: transcription factor ssl1; putative secreted protein 3119676 4609531 azo2840 Azoarcus sp. BH72 putative secreted protein YP_934343.1 3119266 R 62928 CDS YP_934344.1 119899131 4607641 complement(3119803..3120210) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to an orf of the uncultured bacterium 463 of 33% (tremblnew|AAS07979). no domains predicted. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 3120210 4607641 azo2841 Azoarcus sp. BH72 hypothetical protein YP_934344.1 3119803 R 62928 CDS YP_934345.1 119899132 4607642 3120337..3121779 1 NC_008702.1 catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate kinase 3121779 pykA 4607642 pykA Azoarcus sp. BH72 pyruvate kinase YP_934345.1 3120337 D 62928 CDS YP_934346.1 119899133 4609667 3121901..3122965 1 NC_008702.1 class II aldolase; catalyzes the reversible aldol condensation of dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis and gluconeogenesis; fructose-1,6-bisphosphate aldolase 3122965 fba 4609667 fba Azoarcus sp. BH72 fructose-1,6-bisphosphate aldolase YP_934346.1 3121901 D 62928 CDS YP_934347.1 119899134 4608729 complement(3123031..3125097) 1 NC_008702.1 Quinoprotein ethanol dehydrogenase precursor (QEDH). Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol. 73% Bac_PQQ.IPR002372; Bac_PQQ_repeat. Pfam:PF01011; PQQ; 2. Signal peptide:present.; High confidence in function and specificity; putative quinoprotein ethanol dehydrogenase 3125097 exaA1 4608729 exaA1 Azoarcus sp. BH72 putative quinoprotein ethanol dehydrogenase YP_934347.1 3123031 R 62928 CDS YP_934348.1 119899135 4608689 complement(3125178..3126107) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to qbdB of Pseudomonas putida of 56% (gi|22779360|dbj|BAC15558.1|(NBCI ENTREZ)). No domains predicted. No TMHs. Signal peptide present.,; Conserved hypothetical protein; hypothetical protein 3126107 qbdB2 4608689 qbdB2 Azoarcus sp. BH72 hypothetical protein YP_934348.1 3125178 R 62928 CDS YP_934349.1 119899136 4609678 3126355..3128244 1 NC_008702.1 Similar to TREMBL:Q9F9U3 (44% identity); TREMBL:Q88H93 (39% identity); SWISSPROT:P28614 (35% identity). InterPro (IPR002078): Sigma-54 factor interaction domain. InterPro (IPR002197): Helix-turn-helix, Fis-type. InterPro (IPR003593): AAA ATPase. Pfam (PF02954): Bacterial regulatory protein, Fis family. Pfam (PF00158): Sigma-54 interaction domain. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; sigma-54-dependent transcriptional regulator 3128244 4609678 azo2846 Azoarcus sp. BH72 sigma-54-dependent transcriptional regulator YP_934349.1 3126355 D 62928 CDS YP_934350.1 119899137 4607643 3128356..3129030 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3129030 4607643 azo2847 Azoarcus sp. BH72 hypothetical protein YP_934350.1 3128356 D 62928 CDS YP_934351.1 119899138 4607644 3129035..3129451 1 NC_008702.1 Putative AIG2-like protein. Homology to AIG2 of a. thaliana of 27% (sprot|AIG2_ARATH) AIG2 is an Arabidopsis proteins that exhibit RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. Pfam: AIG2-like family singal peptide no TMHs; putative AIG2-like protein 3129451 4607644 azo2848 Azoarcus sp. BH72 putative AIG2-like protein YP_934351.1 3129035 D 62928 CDS YP_934352.1 119899139 4607645 complement(3129466..3131328) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to AQ_563 of Aquifex aeolicus of 37% (trembl|O66835(SRS)). Has IPR003660;PF00672;HAMP domain:This domain is known as the HAMP domain for histidine kinases, adenylyl cyclases,methyl binding proteins and phosphatases. It is found in bacterial sensor and chemotaxis proteins and in eukaryotic histidine kinases. The bacterial proteins are usually integral membrane proteins and part of a two-component signal transduction pathway. Has IPR000160(GGDEF)domain,this domain is found linked to a wide range of non-homologous domains in a variety of bacteria. The function of this domain is unknown, however it has been shown to be homologous to the adenylyl cyclase catalytic domain. This prediction correlates with the functional information available on two GGDEF-containing proteins, namely diguanylate cyclase and phosphodiesterase A of Acetobacter xylinum, both of which regulate the turnover of cyclic diguanosine monophosphate. TMHMM reporting 1 TMH present. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 3131328 4607645 azo2849 Azoarcus sp. BH72 hypothetical protein YP_934352.1 3129466 R 62928 CDS YP_934353.1 119899140 4607646 complement(3131436..3133073) 1 NC_008702.1 Proline/betaine transporter (Proline porter II) (PPII). STRETCH-INACTIVATED PROLINE/BETAINE TRANSPORTER. PROP IS BOTH AN OSMOSENSOR AND AN OSMOREGULATOR WHICH IS AVAILABLE TO PARTICIPATE EARLY IN THE BACTERIAL OSMOREGULATORY RESPONSE. MEDIATES THE ACTIVE ACCUMULATION OF SOLUTES SUCH AS PROLINE GLYCINE BETAINE STACHYDRINE PIPECOLIC ACID ECTOINE AND TAURINE.PROBABLE MtbA PROTEIN 2A0106: citrate-proton symport; Specificity unclear; putative sugar transport protein 3133073 4607646 azo2850 Azoarcus sp. BH72 putative sugar transport protein YP_934353.1 3131436 R 62928 CDS YP_934354.1 119899141 4607647 3133259..3134608 1 NC_008702.1 Conserved hypothetical glycerophosphoryl diester phosphodiesterase. Homology to TdenA01001091 of Thiobacillus denitrificans of 66% (gnl|keqq|mca:MCA1922(KEGG)).InterPro: Glycerophosphoryl diester phosphodiesterase (IPR004129). Pfam: Glycerophosphoryl diester phosphodiesterase. signal peptide. no TMHs; glycerophosphoryl diester phosphodiesterase 3134608 4607647 azo2851 Azoarcus sp. BH72 glycerophosphoryl diester phosphodiesterase YP_934354.1 3133259 D 62928 CDS YP_934355.1 119899142 4607648 complement(3134727..3136160) 1 NC_008702.1 Probable aldehyde dehydrogenase (NAD+). Homology to cddD of R. ruber SC1 of 59% (trembl|Q938F1) Aldehyde dehydrogenases are enzymes which oxidize a wide variety of aliphatic and aromatic aldehydes using NADP as a cofactor. Pfam: Aldehyde dehydrogenase family no TMHs no signal peptide; Family membership; aldehyde dehydrogenase 3136160 cddD 4607648 cddD Azoarcus sp. BH72 aldehyde dehydrogenase YP_934355.1 3134727 R 62928 CDS YP_934356.1 119899143 4608460 3136366..3136650 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA3284 of Pseudomonas aeruginosa of 40% (trembl|Q9HYV9(SRS)). No domains predicted. Signal peptide present. No TMH Present.; Conserved hypothetical protein; hypothetical protein 3136650 4608460 azo2853 Azoarcus sp. BH72 hypothetical protein YP_934356.1 3136366 D 62928 CDS YP_934357.1 119899144 4607649 3136724..3137569 1 NC_008702.1 Conserved hypothetical protein. Homology to PA3283 of P.aeruginosa of 54% (trembl:Q9HYW0). Has PF05114,Protein of unknown function (DUF692);IPR007801:This family consists of several uncharacterised bacterial proteins. No signal peptide or TMH present.; hypothetical protein 3137569 4607649 azo2854 Azoarcus sp. BH72 hypothetical protein YP_934357.1 3136724 D 62928 CDS YP_934358.1 119899145 4607650 3137566..3138312 1 NC_008702.1 Conserved hypothetical protein. Homology to PP2399 of P.putida of 36% (trembl:Q88K92). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3138312 4607650 azo2855 Azoarcus sp. BH72 hypothetical protein YP_934358.1 3137566 D 62928 CDS YP_934359.1 119899146 4607651 3138309..3138881 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA4104 of Pseudomonas aeruginosa of 46% (trembl|Q9HWS4(SRS)). No domains predictec. no signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 3138881 4607651 azo2856 Azoarcus sp. BH72 hypothetical protein YP_934359.1 3138309 D 62928 CDS YP_934360.1 119899147 4607652 3138878..3139468 1 NC_008702.1 Bacteria have multiple sigma factors which are active under specific conditions; the sigma factor binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; RNA polymerase sigma factor 3139468 sigX 4607652 sigX Azoarcus sp. BH72 RNA polymerase sigma factor YP_934360.1 3138878 D 62928 CDS YP_934361.1 119899148 4609869 3139462..3139650 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2972 of C.violaceum of 51% (trembl:Q7NTT1). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3139650 4609869 azo2858 Azoarcus sp. BH72 hypothetical protein YP_934361.1 3139462 D 62928 CDS YP_934362.1 119899149 4607653 3139801..3140484 1 NC_008702.1 Probable carboxylesterase. Homology to estB of P. flourescens of 41% (sprot|EST2_PSEFL). Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity. InterPro: Phospholipase/Carboxylesterase(IPR003140); Esterase/lipase/ thioesterase family acitve site (IPR000379) Pfam: Phospolipase/Carboxylesterase no signal peptide no TMHs; High confidence in function and specificity; carboxylesterase 3140484 estB 4607653 estB Azoarcus sp. BH72 carboxylesterase YP_934362.1 3139801 D 62928 CDS YP_934363.1 119899150 4608675 complement(3140490..3141521) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PJS6w01004232 of Polaromonas sp. JS666 of 53% (gi|54028976|ref|ZP_00361120.1|(NBCI ENTREZ)). InterPro:IPR002035; VWF_A. The von Willebrand factor is a large multimeric glycoprotein found in blood plasma. Mutant forms are involved in the aetiology of bleeding disorders. In von Willebrand factor, the type A domain (vWF) is the prototype for a protein superfamily. Pfam:PF00092; VWA; 1. SMART:SM00327; VWA; 1. PROSITE:PS50234; VWFA; MIP: MIP family channel proteins. No signal peptide present. No. of TMH's: 3 (TMHMM predicted).; Conserved hypothetical protein; hypothetical protein 3141521 4608675 azo2860 Azoarcus sp. BH72 hypothetical protein YP_934363.1 3140490 R 62928 CDS YP_934364.1 119899151 4607654 complement(3141518..3142456) 1 NC_008702.1 Hypothetical protein Rv1480/MT1527/Mb1516. TREMBL:Q7MUE8: 33% identity, 52% similarity. This domain is found in a family of prokaryotic proteins that have no known function. Proteins belonging to this family include hypothetical proteins from eubacteria and archaebacteria. Some of these proteins also contain the Von Willebrand factor, type A domain InterPro: IPR002881; DUF58. Pfam: PF01882; DUF58 Absence of transmembrane helices (TMHMM predicted) cmk: cytidylate kinase; Specificity unclear; hypothetical protein 3142456 4607654 azo2861 Azoarcus sp. BH72 hypothetical protein YP_934364.1 3141518 R 62928 CDS YP_934365.1 119899152 4607655 complement(3142431..3143453) 1 NC_008702.1 MoxR protein (MxaR protein). MAY BE INVOLVED IN THE REGULATION OF FORMATION OF ACTIVE METHANOL DEHYDROGENASE. TREMBLnew:CAE55395: 54% identity, 71% similarity InterPro:IPR003593; AAA_ATPase. IPR000523; Mg_chelatse_chII. Pfam PF01078; Mg_chelatase; 1. SMART SM00382; AAA; 1 InterPro IPR003593; AAA_ATPas ruvB: Holliday junction DNA helicase Ru; High confidence in function and specificity; MoxR protein 3143453 moxR2 4607655 moxR2 Azoarcus sp. BH72 MoxR protein YP_934365.1 3142431 R 62928 CDS YP_934366.1 119899153 4609265 complement(3143481..3144263) 1 NC_008702.1 Conserved hypothetical serine protease. Homology to PJS6w01004229 of Polaromonas sp. JS666 of 53% (gi|54028973|ref|ZP_00361117.1|(NBCI ENTREZ)). Interpro: HtrA/DegQ protease family (IPR001940); Serine protease,trypsine family (IPR001254). Pfam: Typsin. probable signal peptide. no TMHs; Conserved hypothetical protein; putative serine protease 3144263 4609265 azo2863 Azoarcus sp. BH72 putative serine protease YP_934366.1 3143481 R 62928 CDS YP_934367.1 119899154 4607656 3144672..3145691 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q9CBL9: 33% identity; 52% similarity The von Willebrand factor is a large multimeric glycoprotein found in bloodplasma. Mutant forms are involved in the aetiology of bleeding disorders. In von Willebrand factor, the type A domain (vWF) is the prototype for a protein superfamily Hypothetical protein MAV335. InterPro:IPR002035; VWF_A. Pfam:PF00092; VWA; 1. SMART:SM00327; VWA 2_A_01_02: Multidrug resistance protein Presence of Signal peptide (SignalP predicted) Presence of 3 transmembrane helices (TMHMM predicted).; Conserved hypothetical protein; hypothetical protein 3145691 4607656 azo2864 Azoarcus sp. BH72 hypothetical protein YP_934367.1 3144672 D 62928 CDS YP_934368.1 119899155 4607657 3145796..3146101 1 NC_008702.1 Conserved hypothetical protein. Homology to CE0355 of C.efficiens of 56% (trembl:Q8FSM8). No domains predicted. No THs. No signal peptide.; hypothetical protein 3146101 4607657 azo2865 Azoarcus sp. BH72 hypothetical protein YP_934368.1 3145796 D 62928 CDS YP_934369.1 119899156 4607658 3146175..3147203 1 NC_008702.1 GGDEF/PAS/PAC-domain containing protein; diguanylate cyclase 3147203 4607658 azo2866 Azoarcus sp. BH72 diguanylate cyclase YP_934369.1 3146175 D 62928 CDS YP_934370.1 119899157 4607659 complement(3147200..3148141) 1 NC_008702.1 Transcriptional regulator, LysR family This protein activates the transcription of the lysA gene encoding diaminopimelate decarboxylase. LysR is also a negative regulator of its own expression. 44% 1 helixturnhelix PF03466 LysR_substrate; 1. HTH reporting nucleic acid binding motif; Specificity unclear; LysR family transcriptional regulator 3148141 4607659 azo2867 Azoarcus sp. BH72 LysR family transcriptional regulator YP_934370.1 3147200 R 62928 CDS YP_934371.1 119899158 4607660 3148259..3149065 1 NC_008702.1 Putative cytoplasmic protein ygiD, 35% identity to TrEMBL;Q8ZLZ3. TrEMBL;Q8XBP3,Q83JK6. Has PF02900,Catalytic LigB subunit of aromatic ring-opening dioxygenase;IPR004183:The LigAB enzyme (a protocatechuate 4,5-dioxygenase), of Sphingomonas paucimobilis oxidizes protocatechuate (or 3,4-dihydroxybenzoic acid, PCA). The enzyme belongs to the class III extradiol-type catecholic dioxygenase family, which catalyzes the ring-opening reaction of protocatechuate and related compounds.; Conserved hypothetical protein; protocatechuate 4,5-dioxygenase 3149065 ygiD 4607660 ygiD Azoarcus sp. BH72 protocatechuate 4,5-dioxygenase YP_934371.1 3148259 D 62928 CDS YP_934372.1 119899159 4610179 3149082..3149636 1 NC_008702.1 Conserved hypothetical cytochrome B561. Homology to cv3278 of C. violaceum of 69% (trembl|Q7NSZ1). Involved in electronen transport. no domains reported. no signal peptide. 4 TMHs; Family membership; cytochrome B561 3149636 4610179 azo2869 Azoarcus sp. BH72 cytochrome B561 YP_934372.1 3149082 D 62928 CDS YP_934373.1 119899160 4607661 3149682..3150251 1 NC_008702.1 Conserved hypothetical YceI like protein. Homology to CV3277 of C.violaceum of 51% (tremble:Q7NSZ2). Has PF04264, YceI like family;(IPR007372): E. coli YceI is a base-induced periplasmic protein. Its function has not yet been characterised. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3150251 4607661 azo2870 Azoarcus sp. BH72 hypothetical protein YP_934373.1 3149682 D 62928 CDS YP_934374.1 119899161 4607662 3150311..3150889 1 NC_008702.1 Conserved hypothetical YceI like protein. Homology to cv3276 of C. violaceum of 69% (trembl|Q7NSZ3(SRS)) Pfam: YceI like family E. coli. YceI is a base-induced periplasmic protein. Its function has not yet been characterised. signal peptide. no TMHs; Family membership; hypothetical protein 3150889 4607662 azo2871 Azoarcus sp. BH72 hypothetical protein YP_934374.1 3150311 D 62928 CDS YP_934375.1 119899162 4607663 3151019..3152311 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE1887 of Nitrosomonas europaea of 39% (trembl|Q82TK0). No domains predicted. No signal peptide. TMHMM2 program predicts presence of 1 TMH's.; Conserved hypothetical protein; hypothetical protein 3152311 4607663 azo2872 Azoarcus sp. BH72 hypothetical protein YP_934375.1 3151019 D 62928 CDS YP_934376.1 119899163 4607664 3152720..3153655 1 NC_008702.1 catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase; phosphoribosylaminoimidazole-succinocarboxamide synthase 3153655 purC 4607664 purC Azoarcus sp. BH72 phosphoribosylaminoimidazole-succinocarboxamide synthase YP_934376.1 3152720 D 62928 CDS YP_934377.1 119899164 4609656 complement(3153726..3154457) 1 NC_008702.1 Conserved hypothetical protein. Homology to AGR_C_1551 of A.tumefaciens of 40% (trembl:Q7D0J2). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3154457 4609656 azo2874 Azoarcus sp. BH72 hypothetical protein YP_934377.1 3153726 R 62928 CDS YP_934378.1 119899165 4607665 complement(3154417..3154797) 1 NC_008702.1 Conserved hypothetical protein, 67% similarity to TrEMBL:Q8UH3. No good homology with hits in the DB particularly at the start. Signal peptide reported present. No TMH present.; Family membership; hypothetical protein 3154797 4607665 azo2875 Azoarcus sp. BH72 hypothetical protein YP_934378.1 3154417 R 62928 CDS YP_934379.1 119899166 4607666 3155005..3155778 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv3790 of C. violaceum of 36% (cvi:CV3790(KEGG). no domains predicted. no signal peptide. 6 TMHs.; Conserved hypothetical protein; hypothetical protein 3155778 4607666 azo2876 Azoarcus sp. BH72 hypothetical protein YP_934379.1 3155005 D 62928 CDS YP_934380.1 119899167 4607667 3155906..3157972 1 NC_008702.1 Oligopeptidase A counts to the Thimet oligopeptidase family which cleave medium sized peptides. Similar to trembl|Q7VZC9 (49%), to trembl|Q9JX57 (48%) and to sprot|OPDA_ECOLI (51%). Pfam (PF01432): Peptidase family M3; Specificity unclear; oligopeptidase A 3157972 prlC 4607667 prlC Azoarcus sp. BH72 oligopeptidase A YP_934380.1 3155906 D 62928 CDS YP_934381.1 119899168 4609630 3157969..3158451 1 NC_008702.1 Low similiraty to glutaredoxin protein Grx. Has a glutathione-disulfide oxidoreductase activity in the presence of nadph and glutathione reductase.Reduces low molecular weight disulfides and proteins (by similarity). Hypothetical protein yjbD. Signal peptide; Function unclear; hypothetical protein 3158451 4609630 azo2878 Azoarcus sp. BH72 hypothetical protein YP_934381.1 3157969 D 62928 CDS YP_934382.1 119899169 4607668 complement(3158471..3161860) 1 NC_008702.1 Putative ammonium transporter with sensory/GGDEF boxes. TREMBL:Q88M14: 50% identity; 65% similarity; TREMBL:Q7UK31: 46% identity; 58% siilarity InterPro:IPR001633; EAL. IPR000160; GGDEF. IPR001610; PAC. IPR000014; PAS_domain. IPR001905; Ammonium_transpt. IPR003594; ATPbind_ATPase Pfam: PF00563; EAL; PF00990; GGDEF; PF00785; PAC; PF00989; PAS; PF00909; Ammonium_transp SMART SM00267; DUF1; SM00052; DUF2; SM00086; PAC; SM00091; PAS amt: ammonium transporter Nonsecretory protein with signal peptide (SignalP predicted). Presence of 12 transmembrane helices (TMHMM predicted).; Specificity unclear; ammonia permease 3161860 amtE 4607668 amtE Azoarcus sp. BH72 ammonia permease YP_934382.1 3158471 R 62928 CDS YP_934383.1 119899170 4608318 3162198..3162959 1 NC_008702.1 Region start changed from 3161811 to 3162171 (-360 bases); exodeoxyribonuclease III 3162959 4608318 azo2880 Azoarcus sp. BH72 exodeoxyribonuclease III YP_934383.1 3162198 D 62928 CDS YP_934384.1 119899171 4607669 3162965..3163681 1 NC_008702.1 Hypothetical protein, has weak homology with other hits in the Database. TrEMBL;Q67LU3(34%). Has Signal Peptide.; hypothetical protein 3163681 4607669 azo2881 Azoarcus sp. BH72 hypothetical protein YP_934384.1 3162965 D 62928 CDS YP_934385.1 119899172 4607670 3163695..3164381 1 NC_008702.1 Putative transcription regulator, Nnr-type.; Family membership; putative transcription regulator, Nnr-type 3164381 nnr 4607670 nnr Azoarcus sp. BH72 putative transcription regulator, Nnr-type YP_934385.1 3163695 D 62928 CDS YP_934386.1 119899173 4609386 3164450..3164641 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RPA1091 of R.palustris of 57% (tremble:CAE26534). No domains predicted. No TMH. signal peptide present.; Conserved hypothetical protein; hypothetical protein 3164641 4609386 azo2883 Azoarcus sp. BH72 hypothetical protein YP_934386.1 3164450 D 62928 CDS YP_934387.1 119899174 4607671 complement(3164700..3165497) 1 NC_008702.1 catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin; dimethyladenosine transferase 3165497 ksgA 4607671 ksgA Azoarcus sp. BH72 dimethyladenosine transferase YP_934387.1 3164700 R 62928 CDS YP_934388.1 119899175 4609107 complement(3165517..3166509) 1 NC_008702.1 catalyzes oxidation of 4-(phosphohydroxy)-L-threonine into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which decarboxylates to form 1-amino-3-(phosphohydroxy)propan-2-one (3-amino-2-oxopropyl phosphate); 4-hydroxythreonine-4-phosphate dehydrogenase 3166509 pdxA 4609107 pdxA Azoarcus sp. BH72 4-hydroxythreonine-4-phosphate dehydrogenase YP_934388.1 3165517 R 62928 CDS YP_934389.1 119899176 4609516 complement(3166514..3167833) 1 NC_008702.1 Putative peptidyl-prolyl cis-trans isomerase. Homology to surA of E. coli of 32% (sprot|SURA_ECOLI). Assist in the folding of extracytoplasmic proteins. Essential for the survival of E.coli in stationary phase. Pfam: PPIC-type PPIASE domain signal peptide no TMHs; Family membership; putative peptidyl-prolyl cis-trans isomerase 3167833 surA 4609516 surA Azoarcus sp. BH72 putative peptidyl-prolyl cis-trans isomerase YP_934389.1 3166514 R 62928 CDS YP_934390.1 119899177 4609910 complement(3167868..3169991) 1 NC_008702.1 Organic solvent tolerance protein precursor.; High confidence in function and specificity; organic solvent tolerance protein 3169991 ostA 4609910 ostA Azoarcus sp. BH72 organic solvent tolerance protein YP_934390.1 3167868 R 62928 CDS YP_934391.1 119899178 4609460 3170525..3171544 1 NC_008702.1 Conserved hypothetical protein. Homology to ne0191 of N. europaea of 61% (trembl|Q82XR3). Pfam: DUF227. no signal peptide. no TMHs.; hypothetical protein 3171544 4609460 azo2888 Azoarcus sp. BH72 hypothetical protein YP_934391.1 3170525 D 62928 CDS YP_934392.1 119899179 4607672 3171570..3172298 1 NC_008702.1 Specificity unclear; nucleotidyltransferase 3172298 4607672 azo2889 Azoarcus sp. BH72 nucleotidyltransferase YP_934392.1 3171570 D 62928 CDS YP_934393.1 119899180 4607673 3172306..3173652 1 NC_008702.1 Xaa-Pro aminopeptidase,; Specificity unclear; Xaa-Pro aminopeptidase 3173652 pepP 4607673 pepP Azoarcus sp. BH72 Xaa-Pro aminopeptidase YP_934393.1 3172306 D 62928 CDS YP_934394.1 119899181 4609523 3173642..3174805 1 NC_008702.1 ubiH; aromatic-ring hydroxylase (flavoprotein monooxygenase) Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone. Ubiquinone biosynthesis pathway, by similarity.; High confidence in function and specificity; 2-octaprenyl-6-methoxyphenol hydroxylase 3174805 ubiH 4609523 ubiH Azoarcus sp. BH72 2-octaprenyl-6-methoxyphenol hydroxylase YP_934394.1 3173642 D 62928 CDS YP_934395.1 119899182 4610061 3174872..3175945 1 NC_008702.1 Conserved hypothetical tRNA-dihydrouridine synthase. Homology to dusB of Azoarcus sp. EbN1 of 81% (gnl|keqq|eba:ebA1152(KEGG)). Pfam: Dihydrouridine synthase (Dus). Members of this family catalyse the reduction of the 5,6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities, which may overlap. Dus 1 (Q9HGN6) from Saccharomyces cerevisiae acts on pre-tRNA-Phe, while Dus 2 (P53720) acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit, requiring NADPH or NADH, and is stimulated by the presence of FAD. Some family members may be targeted to the mitochondria and even have a role in mitochondria. No signal peptide. No TMHs; Conserved hypothetical protein; putative tRNA-dihydrouridine synthase 3175945 dusB 4610061 dusB Azoarcus sp. BH72 putative tRNA-dihydrouridine synthase YP_934395.1 3174872 D 62928 CDS YP_934396.1 119899183 4608656 3175942..3176178 1 NC_008702.1 Putative fis-like DNA-binding protein, 57% Identity to SwissProt;Q8Y231,TrEMBL;Q7P0M2(58% Identity). Has PF02954, Bacterial regulatory protein, Fis family;IPR002197 HTH_Fis; The Factor for Inversion Stimulation (FIS) protein is a regulator of bacterial functions, and binds specifically to weakly related DNA sequences. It activates ribosomal RNA transcription, and is involved in upstream activation of rRNA promoters. The protein has been shown to play a role in the regulation of virulence factors in both Salmonella typhimurium and Escherichia coli. Some of its functions include inhibition of the initiation of DNA replication from the OriC site,and promotion of Hin-mediated DNA inversion.; putative fis-like DNA-binding protein 3176178 4608656 azo2893 Azoarcus sp. BH72 putative fis-like DNA-binding protein YP_934396.1 3175942 D 62928 CDS YP_934397.1 119899184 4607674 3176279..3177862 1 NC_008702.1 involved in de novo purine biosynthesis; bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase 3177862 purH 4607674 purH Azoarcus sp. BH72 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase YP_934397.1 3176279 D 62928 CDS YP_934398.1 119899185 4609660 3177876..3179153 1 NC_008702.1 catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis; phosphoribosylamine--glycine ligase 3179153 purD 4609660 purD Azoarcus sp. BH72 phosphoribosylamine--glycine ligase YP_934398.1 3177876 D 62928 CDS YP_934399.1 119899186 4609657 3179160..3180074 1 NC_008702.1 catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis; coproporphyrinogen III oxidase 3180074 hemF 4609657 hemF Azoarcus sp. BH72 coproporphyrinogen III oxidase YP_934399.1 3179160 D 62928 CDS YP_934400.1 119899187 4608984 3180121..3181881 1 NC_008702.1 Sulfate permease, SulP. Permease member of the MFS superfamily, involved in the transport of sulfate. Putative sulfate transporter ychM. InterPro: IPR002645; STAS. IPR001902; Sulph_transpt. Pfam: PF01740; STAS; 1. PF00916; Sulfate_transp; 1. SignalP: present. HIGH-AFFINITY H+/SULFATE COTRANSPORTER THAT MEDIATES THE UPTAKE OF SULFATE BY PLANT ROOTS FROM LOW CONCENTRATIONS OF SULFATE IN THE SOIL SOLUTION.; High confidence in function and specificity; putative sulfate transporter 3181881 sulP1 4608984 sulP1 Azoarcus sp. BH72 putative sulfate transporter YP_934400.1 3180121 D 62928 CDS YP_934401.1 119899188 4609908 3181964..3182215 1 NC_008702.1 binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity; 30S ribosomal protein S16 3182215 rpsP 4609908 rpsP Azoarcus sp. BH72 30S ribosomal protein S16 YP_934401.1 3181964 D 62928 CDS YP_934402.1 119899189 4609819 3182235..3182741 1 NC_008702.1 Conserved hypothetical 16S rRNA processing protein rimM. Homology to rimM of R. solanacearum of 44% (sprot|RIMM_RALSO). Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. It could be some accessory protein needed for efficient assembly of the 30S subunit. It is needed in a step prior to rbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Pfam: RimM. no signal peptide. no TMHs; High confidence in function and specificity; 16S rRNA processing protein 3182741 rimM 4609819 rimM Azoarcus sp. BH72 16S rRNA processing protein YP_934402.1 3182235 D 62928 CDS YP_934403.1 119899190 4609728 3182723..3183535 1 NC_008702.1 methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA; tRNA (guanine-N(1)-)-methyltransferase 3183535 trmD 4609728 trmD Azoarcus sp. BH72 tRNA (guanine-N(1)-)-methyltransferase YP_934403.1 3182723 D 62928 CDS YP_934404.1 119899191 4609973 3183621..3184007 1 NC_008702.1 this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site; 50S ribosomal protein L19 3184007 rplS 4609973 rplS Azoarcus sp. BH72 50S ribosomal protein L19 YP_934404.1 3183621 D 62928 CDS YP_934405.1 119899192 4609778 complement(3184128..3185204) 1 NC_008702.1 TREMBL:Q8XWQ6: 39% identity, 56% similarity Hypothetical protein HI1703. InterPro: IPR005495; YjgP_YjgQ. Pfam: PF03739;Pfam :DUF214:Predicted permease Members of this family are predicted integral membrane proteins of unknown function. They are about 350 amino acids long, contain about 6 transmembrane regions and may be permeases, although there is no verification of this. subcellular location:integral membrane protein (potential). similarity:strong, to h.influenzae hi1703 2a69: Auxin Efflux Carrier TMHMM predicted 6 transmembrane helices; Conserved hypothetical protein; integral membrane protein 3185204 4609778 azo2902 Azoarcus sp. BH72 integral membrane protein YP_934405.1 3184128 R 62928 CDS YP_934406.1 119899193 4607675 complement(3185201..3186280) 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q8XWQ7: 40% identity; 55% similarity: probable integral membrane protein InterPro:IPR005495; YjgP_YjgQ. Pfam:PF03739; YjgP_YjgQ :UbiA : UbiA prenyltransferase family Pfam:DUF112:Integral membrane protein DUF112 Signal peptide present (Signal P predicted). Transmembrane helices: 5 (TMHMM predicted).; Conserved hypothetical protein; hypothetical protein 3186280 4607675 azo2903 Azoarcus sp. BH72 hypothetical protein YP_934406.1 3185201 R 62928 CDS YP_934407.1 119899194 4607676 3186423..3187925 1 NC_008702.1 catalyzes the removal of N-terminal amino acids preferably leucine from various peptides; leucyl aminopeptidase 3187925 pepA 4607676 pepA Azoarcus sp. BH72 leucyl aminopeptidase YP_934407.1 3186423 D 62928 CDS YP_934408.1 119899195 4609519 3187931..3188368 1 NC_008702.1 Putative DNA polymerase III chi subunit, 40% Identity to TrEMBL; Q8XWQ9,Q63WC2,Q62LH1. Has PF04364, DNA polymerase III chi subunit, HolC;IPR007459, DNA_pol3_chi; The DNA polymerase III holoenzyme is the polymerase responsible for the replication of the Escherichia coli chromosome. The holoenzyme is composed of the DNA polymerase III core, the sliding clamp, and the DnaX clamp loading complex. The DnaX complex contains either either the tau or gamma product of gene dnax, complexed to delta.delta' and to chi psi. Chi forms a 1:1 heterodimer with psi. The chi psi complex functions by increasing the affinity of tau and gamma for delta.delta' allowing a functional clamp-loading complex to form at physiological subunit concentrations. Psi is responsible for the interaction with DnaX (gamma/tau), but psi is insoluble unless it is in a complex with chi.; DNA-directed DNA polymerase 3188368 holC 4609519 holC Azoarcus sp. BH72 DNA-directed DNA polymerase YP_934408.1 3187931 D 62928 CDS YP_934409.1 119899196 4609014 3188383..3188892 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA7176 of Azoarcus sp. EbN1 of 44% (gnl|keqq|eba:ebA7176(KEGG)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3188892 4609014 azo2906 Azoarcus sp. BH72 hypothetical protein YP_934409.1 3188383 D 62928 CDS YP_934410.1 119899197 4607677 3188987..3191983 1 NC_008702.1 valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain; valyl-tRNA synthetase 3191983 valS 4607677 valS Azoarcus sp. BH72 valyl-tRNA synthetase YP_934410.1 3188987 D 62928 CDS YP_934411.1 119899198 4610089 complement(3192016..3192657) 1 NC_008702.1 Putative TetR family transcriptional regulator,; Family membership; TetR family transcriptional regulator 3192657 4610089 azo2908 Azoarcus sp. BH72 TetR family transcriptional regulator YP_934411.1 3192016 R 62928 CDS YP_934412.1 119899199 4607678 complement(3192663..3193847) 1 NC_008702.1 Similar to TREMBL:Q82VH0 (64% identity); TREMBL:Q9HTW0 (63% identity); TREMBL:Q8FZX1 (64% identity). TMHMM predicting seven transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Specificity unclear; ABC transporter permease 3193847 4607678 azo2909 Azoarcus sp. BH72 ABC transporter permease YP_934412.1 3192663 R 62928 CDS YP_934413.1 119899200 4607679 complement(3193878..3196646) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:Q8FZX0 (58% identity); TREMBL:Q8U6Y9 (59% identity). InterPro (IPR003593): AAA ATPase. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003439): ABC transporter. Pfam (PF00005): ABC transporter. TMHMM predicting five transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; High confidence in function and specificity; ABC transporter permease and ATP-binding protein 3196646 4607679 azo2910 Azoarcus sp. BH72 ABC transporter permease and ATP-binding protein YP_934413.1 3193878 R 62928 CDS YP_934414.1 119899201 4607680 complement(3196643..3197716) 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family, TREMBL:Q8ZLD9 (56% identity); TREMBL:Q8VR08 (56% identity). Pfam (PF00529): HlyD family secretion protein. SignalP reporting signal peptide. TC (8.A.1): The Membrane Fusion Protein (MFP) Family.; Family membership; membrane fusion protein 3197716 4607680 azo2911 Azoarcus sp. BH72 membrane fusion protein YP_934414.1 3196643 R 62928 CDS YP_934415.1 119899202 4607681 complement(3197732..3199141) 1 NC_008702.1 Probable outer membrane efflux protein. Homology to oprM of P. aeruginosa of 47%. Component of an efflux system that confers multidrug or multible antibiotic resistence. InterPro: Outer membrane efflux protein Pfam: Outer membrane efflux protein signal peptide no TMHs; Family membership; outer membrane efflux protein 3199141 oprM3 4607681 oprM3 Azoarcus sp. BH72 outer membrane efflux protein YP_934415.1 3197732 R 62928 CDS YP_934416.1 119899203 4609455 3199640..3200071 1 NC_008702.1 In P. aeruginosa fimT and fimU genes contain prepilin-like leader sequences. FimT plays probable a role in fimbrial biogenesis. Similar to trembl|Q87FA9 (30%). Pfam (PF00114): Pilin (bacterial filament) TMHMM reporting one TMH. SignalP reporting Signal peptide.; Function unclear; putative pre-pilin like protein 3200071 fimT 4609455 fimT Azoarcus sp. BH72 putative pre-pilin like protein YP_934416.1 3199640 D 62928 CDS YP_934417.1 119899204 4608763 3200061..3200528 1 NC_008702.1 In P. aeruginosa the genes pilV and pilE encode prepilin-like proteins involved in type 4 fimbrial biogenesis. Similar to trembl|Q8PJ74 (46%). SignalP reporting Signal Peptide.; Function unclear; putative prepilin-like protein 3200528 pilV 4608763 pilV Azoarcus sp. BH72 putative prepilin-like protein YP_934417.1 3200061 D 62928 CDS YP_934418.1 119899205 4609576 3200534..3201547 1 NC_008702.1 Conserved hypothetical protein; hypothetical protein 3201547 4609576 azo2915 Azoarcus sp. BH72 hypothetical protein YP_934418.1 3200534 D 62928 CDS YP_934419.1 119899206 4607682 3201544..3202113 1 NC_008702.1 In P. aeruginosa the PilW and PilX proteins are membrane located, possess the hydrophobic N-terminus characteristic of prepilin-like proteins, and appear to belong to the GspJ and GspK group of proteins that are required for protein secretion in a wide range of Gram-negative bacteria. These genes are involved in the assembly of type 4 fimbriae. Similar to trembl|Q8PJ76 (34%). TMHMM reporting one TMH. SignalP reporting Signal peptide.; Function unclear; putative Tfp pilus assembly protein 3202113 pilX 4607682 pilX Azoarcus sp. BH72 putative Tfp pilus assembly protein YP_934419.1 3201544 D 62928 CDS YP_934420.1 119899207 4609578 3202121..3206329 1 NC_008702.1 PilY1 is a large protein with C-terminal homology to the PilC2 protein of Neisseria gonorrhoeae, thought to be a fimbrial tip-associated adhesin, and which, like PilY1, is involved in fimbrial assembly. PilY1 appears to be located in both the membrane and the external fimbrial fractions. Similar to pir|H82802 (39%). SignalP reporting Signal peptide; Function unclear; putative Tfp pilus assembly protein 3206329 pilY1B 4609578 pilY1B Azoarcus sp. BH72 putative Tfp pilus assembly protein YP_934420.1 3202121 D 62928 CDS YP_934421.1 119899208 4609580 3206332..3206736 1 NC_008702.1 In P. aeruginosa PilE is probable involved in pilus biogenesis and in twitching motility. Similar to trembl|Q82TX3 (50%) and to pir|G82857 (43%). Pfam (PF00114): Pilin (bacterial filament) SignalP reporting Signal peptide. TMHMM reporting one Tmhelix; Function unclear; putative prepilin like protein 3206736 pilE 4609580 pilE Azoarcus sp. BH72 putative prepilin like protein YP_934421.1 3206332 D 62928 CDS YP_934422.1 119899209 4609559 complement(3206799..3207029) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc0810 of R. solanacearum of 71% (trembl|Q8Y180). InterPro: Uncharacterized protein family UPF0033 (IPR001455). Pfam: Uncharacterized protein family UPF0033. no signal peptide. no TMH; Family membership; hypothetical protein 3207029 4609559 azo2919 Azoarcus sp. BH72 hypothetical protein YP_934422.1 3206799 R 62928 CDS YP_934423.1 119899210 4607683 complement(3207107..3208546) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv0524 C. violaceum of 42% (trembl|Q7P0P2). Pfam: Peptidase family M48. signal peptide. no TMHs; peptidase 3208546 4607683 azo2920 Azoarcus sp. BH72 peptidase YP_934423.1 3207107 R 62928 CDS YP_934424.1 119899211 4607684 3208627..3209139 1 NC_008702.1 Molybdenum cofactor biosynthesis protein C. Together with moaA is involved in the conversion of a guanosine derivative (GXP) into molybdopterin precursor Z (By similarity). InterPro: MoaC family moaC: molybdenum cofactor biosynthesis protein C.; High confidence in function and specificity; molybdenum cofactor biosynthesis protein C 3209139 moaC 4607684 moaC Azoarcus sp. BH72 molybdenum cofactor biosynthesis protein C YP_934424.1 3208627 D 62928 CDS YP_934425.1 119899212 4609242 3209384..3209602 1 NC_008702.1 Conserved hypothetical protein. Homology to orf11 of A. vinelandii of 63% (trembl|Q44537). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 3209602 4609242 azo2922 Azoarcus sp. BH72 hypothetical protein YP_934425.1 3209384 D 62928 CDS YP_934426.1 119899213 4607685 complement(3209702..3210415) 1 NC_008702.1 Probable ATP-binding component of tungstate ABC transporter. Homology to tupC of E. acidaminophilum of 40% (tremble: Q93KD4). PROBABLY PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR tungstate. PROBABLY RESPONSIBLE FOR ENERGY COUPLING TO THE TRANSPORT SYSTEM. Pfam: ABC transporter Interpo: ABC-transporter (IPR003439), AAA-ATPase (IPR003593), ATP/GTP-binding site motif A (loopP) (IPR001687) no signal peptide no TMHS; Family membership; tungstate ATP-binding protein 3210415 tupC 4607685 tupC Azoarcus sp. BH72 tungstate ATP-binding protein YP_934426.1 3209702 R 62928 CDS YP_934427.1 119899214 4610051 complement(3210426..3211130) 1 NC_008702.1 Probable tungstate permease. Homology to tupB of E. acidaminophilum of 40% (tremble: Q93KD5) Permase of an abc-transport system involved in transport of oxyanions. no signal peptide probable 5 TMHs; Family membership; tungstate permease 3211130 tupB 4610051 tupB Azoarcus sp. BH72 tungstate permease YP_934427.1 3210426 R 62928 CDS YP_934428.1 119899215 4610050 3211273..3211878 1 NC_008702.1 Hypothetical secredted protein. no homology of the entire protein to the data bank. no domains predicted signal peptide no TMHs; hypothetical protein 3211878 4610050 azo2925 Azoarcus sp. BH72 hypothetical protein YP_934428.1 3211273 D 62928 CDS YP_934429.1 119899216 4607686 3211901..3212554 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to orf of P.aeruginosa of 30% (ZP_00137401). No domains predicted. Signal peptide reported present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 3212554 4607686 azo2926 Azoarcus sp. BH72 hypothetical protein YP_934429.1 3211901 D 62928 CDS YP_934430.1 119899217 4607687 complement(3212572..3212877) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA5010 of Azoarcus sp. EbN1 of 40% (gnl|keqq|eba:ebA5010(KEGG)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3212877 4607687 azo2927 Azoarcus sp. BH72 hypothetical protein YP_934430.1 3212572 R 62928 CDS YP_934431.1 119899218 4607688 complement(3212888..3213139) 1 NC_008702.1 Conserved hypothetical protein. Homology to Rgel02003612 of Rubrivivax gelatinosus PM1 of 51% (gi|47572157|ref|ZP_00242203.1|(NBCI ENTREZ)). Pfam: This family ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. This family of proteins have two conserved Glycines at the COOH terminus. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end. no signal peptide. no TMHs; hypothetical protein 3213139 4607688 azo2928 Azoarcus sp. BH72 hypothetical protein YP_934431.1 3212888 R 62928 CDS YP_934432.1 119899219 4607689 complement(3213150..3214418) 1 NC_008702.1 Putative oxidoreductase. Homology to narC of C. perfringens of 27% (trembl|Q9WX91) InterPro: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (IPR001327) Pfam: Pyridine nucleotide-disulfide oxidoreductase no signal peptide no TMHs purD: phosphoribosylamine--glycine lig; Family membership; putative oxidoreductase 3214418 4607689 azo2929 Azoarcus sp. BH72 putative oxidoreductase YP_934432.1 3213150 R 62928 CDS YP_934433.1 119899220 4607690 complement(3214510..3216357) 1 NC_008702.1 Probable aldehyde ferredoxin oxidoreductase. Homology to aorA of E. acidodaminophilum of 47% (trembl|Q93KC8) InterPro: Aldehyde ferredoxin oxidoreductase (IPR001203) Pfam: Aldehyde ferredoxin oxidoreductase, N-terminus; Aldeyhde ferredoxin oxidoreductase, damains 2&3 no TMHs no signal peptide; High confidence in function and specificity; aldehyde ferredoxin oxidoreductase 3216357 aorA 4607690 aorA Azoarcus sp. BH72 aldehyde ferredoxin oxidoreductase YP_934433.1 3214510 R 62928 CDS YP_934434.1 119899221 4608322 complement(3216368..3216844) 1 NC_008702.1 Conserved hypothetical protein. Homology to gsu0911 of G. sulfurreducens of 38% (tremblnew|AAR34238). Pfam: 4Fe-4S binding domain. no signal peptide. no TMHs; hypothetical protein 3216844 4608322 azo2931 Azoarcus sp. BH72 hypothetical protein YP_934434.1 3216368 R 62928 CDS YP_934435.1 119899222 4607691 complement(3216979..3219003) 1 NC_008702.1 Similar to TREMBL:Q8XUQ7 (42% identity); TREMBL:Q8PPN1 (40% identity); SWISSPROT:P28614 (37% identity). Pfam (PF02954): Bacterial regulatory protein,Fis family. Pfam (PF00158): Sigma-54 interaction domain. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; sigma-54 dependent transcriptional regulator 3219003 4607691 azo2932 Azoarcus sp. BH72 sigma-54 dependent transcriptional regulator YP_934435.1 3216979 R 62928 CDS YP_934436.1 119899223 4607692 3219326..3219763 1 NC_008702.1 Probable cytochrome c550. Homology to exaB of P. aeruginosa of 57% (trembl|Q9Z4P8) Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation system InterPro: Cytochrome c class I ((IPR003088) Pfam: Cytochrome c signal peptide no TMHs; High confidence in function and specificity; cytochrome c550 3219763 exaB1 4607692 exaB1 Azoarcus sp. BH72 cytochrome c550 YP_934436.1 3219326 D 62928 CDS YP_934437.1 119899224 4608694 3219753..3220616 1 NC_008702.1 Conserved hypothetical amino acid-binding protein. Homology to pp2676 of P. putida of 34% (trembl|Q88JH3). PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR amino acids. InterPro: Bacterial extracellular solute-binding proteins family 3 (IPR001638); solute binding protein /glutamate receptor (IPR001311). Pfam: Bacterial extracellular solute-binding protein. singal peptide. no TMHs; Specificity unclear; amino acid-binding protein 3220616 4608694 azo2934 Azoarcus sp. BH72 amino acid-binding protein YP_934437.1 3219753 D 62928 CDS YP_934438.1 119899225 4607693 3220743..3221693 1 NC_008702.1 Conserved hypothetical protein TMHMM2 reporting the presence of 1 TMH's. No Signal peptide present. Has 2 WD40 repeats;SMART;SM00320, Beta-transducin (G-beta) is one of the three subunits (alpha, beta, and gamma) of the guanine nucleotide-binding proteins (G proteins) which act as intermediaries in the transduction of signals generated by transmembrane receptors (see IPR001632). The alpha subunit binds to and hydrolyzes GTP; the functions of the beta and gamma subunits are less clear but they seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition.; Function unclear; hypothetical protein 3221693 4607693 azo2935 Azoarcus sp. BH72 hypothetical protein YP_934438.1 3220743 D 62928 CDS YP_934439.1 119899226 4607694 3221690..3222226 1 NC_008702.1 Conserved hypothetical cytochrome B561. Homology to cv3278 of C. violaceum of 41%. Involved in electronen transfer. no domains. no signal peptide. 4 TMHs; Family membership; cytochrome B561 3222226 4607694 azo2936 Azoarcus sp. BH72 cytochrome B561 YP_934439.1 3221690 D 62928 CDS YP_934440.1 119899227 4607695 complement(3222295..3222618) 1 NC_008702.1 Probable cytochrome c-552 precursor. Homology to cyt of N. europaea of 50% (sprot|C552_NITEU). ELECTRON DONOR FOR CYTOCHROME CD1 IN NITRITE AND NITRATE RESPIRATION. InterPro: Cytochrome c class I (IPR003088); Cytochrome c, class ID (IPR002324); cytochrome c, class IC (IPR008168) Pfam: cytochrome c signal peptide no TMHs; High confidence in function and specificity; cytochrome c-552 precourser 3222618 cyt1 4607695 cyt1 Azoarcus sp. BH72 cytochrome c-552 precourser YP_934440.1 3222295 R 62928 CDS YP_934441.1 119899228 4608567 complement(3222672..3223040) 1 NC_008702.1 Conserved hypothetical protein. Homology to OB2533 of O.iheyensis of 64% (trembl:Q8ENF0). Has PF05610,Protein of unknown function (DUF779);IPR008497;This family consists of several bacterial proteins of unknown function. No signal peptide or TMH present.; hypothetical protein 3223040 4608567 azo2938 Azoarcus sp. BH72 hypothetical protein YP_934441.1 3222672 R 62928 CDS YP_934442.1 119899229 4607696 complement(3223255..3224775) 1 NC_008702.1 Aldehyde dehydrogenase (NAD+). Homology to aldA of X. autotrophicus of 76% (trembl|O50203). Aldehyde dehydrogenases are enzymes which oxidize a wide variety of aliphatic and aromatic aldehydes using NADP as a cofactor. InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no TMHs; High confidence in function and specificity; aldehyde dehydrogenase 3224775 aldA 4607696 aldA Azoarcus sp. BH72 aldehyde dehydrogenase YP_934442.1 3223255 R 62928 CDS YP_934443.1 119899230 4608294 3225116..3226705 1 NC_008702.1 Similar to TREMBL:Q7P122 (29% identity); TREMBL:Q87YQ3 (29% identity); SWISSPROT:P28614 (28% identity). Pfam (PF00158): Sigma-54 interaction domain.; Specificity unclear; sigma-54 dependent transcriptional regulator 3226705 4608294 azo2940 Azoarcus sp. BH72 sigma-54 dependent transcriptional regulator YP_934443.1 3225116 D 62928 CDS YP_934444.1 119899231 4607697 complement(3226773..3227456) 1 NC_008702.1 Zinc transporter zitB (formaly ybgR).Involved in zinc efflux across the cytoplasmic membrane thus reducing zinc accumulation in the cytoplasm and rendering bacteria more resistant to zinc. It may contribute to zinc homeostasis at low concentrations of zinc (By similarity).The E.coli zitB gene encodes a Zn(II) transporter belonging to the cation diffusion facilitator family.; High confidence in function and specificity; putative zinc transport protein 3227456 zitB 4607697 zitB Azoarcus sp. BH72 putative zinc transport protein YP_934444.1 3226773 R 62928 CDS YP_934445.1 119899232 4610201 3227685..3229697 1 NC_008702.1 Conserved hypothetical protein. Homology to PSPTO5055 of P.syringae of 36% (trembl:Q87V84). No domains predicted. No signal peptide or TMH reported present.; hypothetical protein 3229697 4610201 azo2942 Azoarcus sp. BH72 hypothetical protein YP_934445.1 3227685 D 62928 CDS YP_934446.1 119899233 4607698 3230033..3231325 1 NC_008702.1 Conserved hypothetical protein. Homology to PSPTO5054 of P.syringae of 35% (trembl:Q87V85). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3231325 4607698 azo2943 Azoarcus sp. BH72 hypothetical protein YP_934446.1 3230033 D 62928 CDS YP_934447.1 119899234 4607699 complement(3231339..3231797) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ws0556 of W. succinogenes of 51% (trembl|Q7MSD6). Pfam: DUF411. The function of the members of this bacterial protein family is unknown. Some members may be involved in conferring cation resistance. signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 3231797 4607699 azo2944 Azoarcus sp. BH72 hypothetical protein YP_934447.1 3231339 R 62928 CDS YP_934448.1 119899235 4607700 complement(3231842..3233239) 1 NC_008702.1 Two component sensor kinase,; High confidence in function and specificity; two component sensor kinase 3233239 copS 4607700 copS Azoarcus sp. BH72 two component sensor kinase YP_934448.1 3231842 R 62928 CDS YP_934449.1 119899236 4608515 complement(3233236..3233916) 1 NC_008702.1 Transcriptional activator protein,; High confidence in function and specificity; two component response regulator 3233916 copR 4608515 copR Azoarcus sp. BH72 two component response regulator YP_934449.1 3233236 R 62928 CDS YP_934450.1 119899237 4608514 3234103..3234528 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to pp5391 of P. putida of 31% (trembl|Q88BZ2). no domains predicted. signal peptide. no TMHs.; Conserved hypothetical protein; hypothetical protein 3234528 4608514 azo2947 Azoarcus sp. BH72 hypothetical protein YP_934450.1 3234103 D 62928 CDS YP_934451.1 119899238 4607701 3234590..3234883 1 NC_008702.1 AAH:63279: 42% identity, 55% similarity Anti-oncogene; SH3 domain; Repeat; Description:SAM and SH3 domains containing protein 1 (proline-glutamate repeat-containing protein). Gene name(s) sash1 or pepe1 contains 1 SH3 domain. similarity: contains 2 sterile alpha motif (SAM) domains May have a role in a signaling pathway. Could act as a tumor supressor. InterPro: IPR001660; SAM. IPR001452: SH3. Pfam:PF00536; SAM; 2. PF00018:SH3; 1. SMART:SM00454; SAM; Function unclear; putative hydrolase 3234883 4607701 azo2948 Azoarcus sp. BH72 putative hydrolase YP_934451.1 3234590 D 62928 CDS YP_934452.1 119899239 4607702 3234880..3236301 1 NC_008702.1 Putative outer membrane efflux protein involved in cobalt-zinc-cadmium resistance (Cation efflux system protein czcC). Homology to czcC of R. eutropha of 24%. czcC protein appears to modify the specificity of the system perhaps by acting on the czcB protein. When the czcC protein is added to czcA and czcB the efflux systems gained specificity for Cd(2+)and Co(2+) signal peptide no TMHs; Family membership; putative metal ion efflux outer membrane protein 3236301 czcC 4607702 czcC Azoarcus sp. BH72 putative metal ion efflux outer membrane protein YP_934452.1 3234880 D 62928 CDS YP_934453.1 119899240 4608572 3236323..3237729 1 NC_008702.1 Multicopper oxidases are enzymes that possess three spectroscopically differentcopper centers. Similar to TREMBL:Q7X4G1 (71% identity); TREMBL:Q8U8U7 (71% identity); TREMBL:Q92ZC7 (70% identity). InterPro (IPR001117): Multicopper oxidase type 1. Pfam (PF00394): Multicopper oxidase. SignalP reporting signal peptide.; Function unclear; multicopper oxidase 3237729 4608572 azo2950 Azoarcus sp. BH72 multicopper oxidase YP_934453.1 3236323 D 62928 CDS YP_934454.1 119899241 4607703 3237804..3238304 1 NC_008702.1 CopC: copper tolerance protein.CopC is a bacterial blue copper protein that binds 1 atom of copper per protein molecule. Along with CopA, CopC mediates copper resistance by sequestration of copper in the periplasm. 43% Cu-oxidase. InterPro:IPR008972; Cupredoxin. Signal pepetide:present. TMhelix:1.; Function unclear; putative copper tolerance protein 3238304 4607703 azo2951 Azoarcus sp. BH72 putative copper tolerance protein YP_934454.1 3237804 D 62928 CDS YP_934455.1 119899242 4607704 3238418..3238759 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to SMc02284 of S. meliloti of 38% (trembl|Q92S41(SRS)). Has PF07076:IPR009780;Protein of unknown function (DUF1344):This family consists of several short,hypothetical bacterial proteins of around 80 residues in length. Members of this family are found in Rhizobium,Agrobacterium and Brucella species. The function of this family is unknown. Signal peptide predicted. No TMHs; Conserved hypothetical protein; hypothetical protein 3238759 4607704 azo2952 Azoarcus sp. BH72 hypothetical protein YP_934455.1 3238418 D 62928 CDS YP_934456.1 119899243 4607705 3238999..3240282 1 NC_008702.1 Conserved hypothetical outer membrane efflux. Homology to ebA2169 Azoarcus sp. EbN1 of 68% (gnl|keqq|eba:ebA2169(KEGG)). Pfam: Outer membrane efflux protein. signal peptide. probable 1 TMH in signal peptide.; Conserved hypothetical protein; outer membrane efflux protein 3240282 4607705 azo2953 Azoarcus sp. BH72 outer membrane efflux protein YP_934456.1 3238999 D 62928 CDS YP_934457.1 119899244 4607706 3240279..3241874 1 NC_008702.1 Putative membrane fusion protein silB precursor. COMPONENT OF THE SIL CATION-EFFLUX SYSTEM THAT CONFERS RESISTANCE TO SILVER. MAY BE PART OF A THREE-COMPONENT CATION/PROTON ANTIPORTER.; High confidence in function and specificity; cation efflux transmembrane protein 3241874 cusB 4607706 cusB Azoarcus sp. BH72 cation efflux transmembrane protein YP_934457.1 3240279 D 62928 CDS YP_934458.1 119899245 4608543 3242221..3246132 1 NC_008702.1 Region start changed from 3242195 to 3242423 (-228 bases); putative cation efflux system protein 3246132 4608543 azo2955 Azoarcus sp. BH72 putative cation efflux system protein YP_934458.1 3242221 D 62928 CDS YP_934459.1 119899246 4607707 3246273..3248684 1 NC_008702.1 Similar to E.coli CopA: copper transporting P-type ATPase protein, involved in the uptake and metabolism of copper(EC 3.6.3.4).Involved in copper transport(By similarity). Putative cation transporting P-type ATPase (EC 3.6.3.-).; High confidence in function and specificity; putative cooper-transporting ATPase 3248684 4607707 azo2956 Azoarcus sp. BH72 putative cooper-transporting ATPase YP_934459.1 3246273 D 62928 CDS YP_934460.1 119899247 4607708 3248752..3249315 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs. HTH predicted; hypothetical protein 3249315 4607708 azo2957 Azoarcus sp. BH72 hypothetical protein YP_934460.1 3248752 D 62928 CDS YP_934461.1 119899248 4607709 complement(3249338..3249823) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3249823 4607709 azo2958 Azoarcus sp. BH72 hypothetical protein YP_934461.1 3249338 R 62928 CDS YP_934462.1 119899249 4607710 3250037..3250312 1 NC_008702.1 Carboxymuconolactone decarboxylase family protein,33% identity to TrEMBL;Q88K69. Has PF02627,Carboxymuconolactone decarboxylase family; Carboxymuconolactone decarboxylase (CMD)is involved in protocatechuate catabolism. In some bacteria a gene fusion event leads to expression of CMD with a hydrolase involved in the same pathway. In these bifunctional proteins (e.g. O67982) CMD represents the C-terminal domain,Abhydrolase_1 represents the N-terminal domain.; Function unclear; 4-carboxymuconolactone decarboxylase 3250312 4607710 azo2959 Azoarcus sp. BH72 4-carboxymuconolactone decarboxylase YP_934462.1 3250037 D 62928 CDS YP_934463.1 119899250 4607711 3250526..3251743 1 NC_008702.1 27% similarity to Hypothetical protein ycxA (ORF5)of Bacillus subtilis. 12 predicted transmembrane helices with signal peptide. 54% identity, 69% similarity to Pseudomonas putida membrane protein. InterPro: General substrate transporters 2A0111: Oxalate/Formate Antiporter; Function unclear; putative transporter 3251743 4607711 azo2960 Azoarcus sp. BH72 putative transporter YP_934463.1 3250526 D 62928 CDS YP_934464.1 119899251 4607712 3251743..3251892 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Has Signal peptide; hypothetical protein 3251892 4607712 azo2961 Azoarcus sp. BH72 hypothetical protein YP_934464.1 3251743 D 62928 CDS YP_934465.1 119899252 4607713 3251919..3252485 1 NC_008702.1 Putative RNA polymerase sigma factor sigZ. The sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. Similar to SWISSPROT: sprot|SIGZ_BACSU (23% Bacillus subtilis, RNA polymerase sigma factor SigZ) / sprot|RPSH_PSEAE (28% Pseudomonas aeruginosa, RNA polymerase sigma-h factor (sigma-30), AlgU or AlgT) InterPro: IPR000838 Sigma70_ECF. Pfam: PF07638 ECF sigma factor.; High confidence in function and specificity; putative RNA polymerase sigma factor SigZ 3252485 sigZ 4607713 sigZ Azoarcus sp. BH72 putative RNA polymerase sigma factor SigZ YP_934465.1 3251919 D 62928 CDS YP_934466.1 119899253 4609870 complement(3252493..3253860) 1 NC_008702.1 Probable chromate transport protein.This protein reduces chromate accumulation and is essential for chromate resistance.Integral membrane protein.Induced by chromate. 27% Chromate_transpt. Pfam:PF02417; Chromate_transp; 2. TIGRFAMs:TIGR00937; 2A51; 1. TMHelix: 11. InterPro: Chromat transporter 2A51: Chromate Ion Transporter; High confidence in function and specificity; putative chromate transport protein 3253860 chrA 4609870 chrA Azoarcus sp. BH72 putative chromate transport protein YP_934466.1 3252493 R 62928 CDS YP_934467.1 119899254 4608480 complement(3253862..3254782) 1 NC_008702.1 Probable Chromate resistance signal peptide protein, 49% Identity to TrEMBL; Q7NZK1, Q8XSC4. Has Signal Pepride.; chromate resistance signal peptide protein 3254782 chrB 4608480 chrB Azoarcus sp. BH72 chromate resistance signal peptide protein YP_934467.1 3253862 R 62928 CDS YP_934468.1 119899255 4608481 complement(3254885..3255571) 1 NC_008702.1 Protein yhhW. SPROT:P46852: 51% identity, 69% similarity entry represents N-terminal domain of Pirin proteins from both eukaryotes and prokaryotes. The function of Pirin is unknown but the gene coding for this protein is known to be expressed in all tissues in the human body InterPro; IPR007113; Cupin_sup. InterPro; IPR003829; Pirin_N. Pfam; PF02678; Pirin; TIGR00292: thiazole biosynthesis enzyme Absence of transmembrane helices (TMHMM predicted).; Function unclear; hypothetical protein 3255571 4608481 azo2965 Azoarcus sp. BH72 hypothetical protein YP_934468.1 3254885 R 62928 CDS YP_934469.1 119899256 4607714 complement(3255634..3256128) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to VPA0043 of Vibrio parahaemolyticus of 64% (trembl|Q87K55). Pfam: TQO small subunit DoxD. DoxD is a subunit of the terminal quinol oxidase present in the plasma membrane of Acidianus ambivalens, with calculated molecular mass of 20.4 kDa. Thiosulphate:quinone oxidoreductase (TQO) is one of the early steps in elemental sulphur oxidation. A novel TQO enzyme was purified from the thermo-acidophilic archaeon Acidianus ambivalens and shown to consist of a large subunit (DoxD) and a smaller subunit (DoxA). The DoxD- and DoxA-like two subunits are fused together in a single polypeptide. Signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 3256128 4607714 azo2966 Azoarcus sp. BH72 hypothetical protein YP_934469.1 3255634 R 62928 CDS YP_934470.1 119899257 4607715 3256251..3257171 1 NC_008702.1 Transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 3257171 hupR 4607715 hupR Azoarcus sp. BH72 LysR family transcriptional regulator YP_934470.1 3256251 D 62928 CDS YP_934471.1 119899258 4609052 3257288..3257887 1 NC_008702.1 SPROT:Q92PU3: 71% identity; 82% similarity Flavoprotein wrbA1. cofactor:binds 1 fmn per monomer (by similarity). similarity:belongs to the wrba family; contains 1 flavodoxin-like domain. InterPro:IPR008254; Flav_nitox_synth. IPR001226; Flavodoxin. Pfam: PF00258; flavodoxin; 1. Absence of signal peptide (Signal P predicted) and transmembrane helices (TMHMM predicted) met_pdase_I: methionine aminopeptidase t; High confidence in function and specificity; trp repressor binding protein 3257887 wrbA1 4609052 wrbA1 Azoarcus sp. BH72 trp repressor binding protein YP_934471.1 3257288 D 62928 CDS YP_934472.1 119899259 4610112 complement(3258728..3259585) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA582 of Azoarcus sp. EbN1 of 37% (gnl|keqq|eba:ebA582(KEGG)). No domains predicted. Signal P reporting signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 3259585 4610112 azo2969 Azoarcus sp. BH72 hypothetical protein YP_934472.1 3258728 R 62928 CDS YP_934473.1 119899260 4607716 complement(3259643..3260290) 1 NC_008702.1 Probable two-component response regulator,; Family membership; two-component response regulator 3260290 uvrY 4607716 uvrY Azoarcus sp. BH72 two-component response regulator YP_934473.1 3259643 R 62928 CDS YP_934474.1 119899261 4610085 complement(3260287..3261633) 1 NC_008702.1 Putative two-component sensor histidine kinase,only very low similarity to SWISSPROT: sprot|DEGS_BACBR (13% Bacillus brevis, DegS) InterPro: IPR003661 His_kinA_N. Pfam: PF00672 HAMP domain. PF02518 Histidine kinase-, DNA gyrase B-, phytochrome-like ATPase TMHMM reporting 2 transmembrane helices. Sensor protein degS (EC 2.7.3.-). INVOLVED IN A SENSORY TRANSDUCTION PATHWAY THAT AFFECT THE PRODUCTION OF ENZYMES THAT DEGRADE POLYMERIC CARBON AND NITROGEN SOURCES. DEGS PROBABLY ACTS AS A KINASE THAT PHOSPHORYLATES DEGU.; Function unclear; putative two-component sensor histidine kinase 3261633 4610085 azo2971 Azoarcus sp. BH72 putative two-component sensor histidine kinase YP_934474.1 3260287 R 62928 CDS YP_934475.1 119899262 4607717 3261821..3263698 1 NC_008702.1 Quinoprotein ethanol dehydrogenase precursor (QEDH). Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol. 73% Bac_PQQ.IPR002372; Bac_PQQ_repeat. Pfam:PF01011; PQQ; 2. Signal peptide:present.; High confidence in function and specificity; ExaA2 protein 3263698 exaA2 4607717 exaA2 Azoarcus sp. BH72 ExaA2 protein YP_934475.1 3261821 D 62928 CDS YP_934476.1 119899263 4608690 3263828..3264388 1 NC_008702.1 Pentapeptide repeat family protein, 36% identity(46% similarity) to TrEMBL;Q88JH6. Signal P reporting Signal Peptide present. Has 3 copies of PF00805,Pentapeptide repeats;IPR001646, 5peptide_repeat;These repeats are found in many cyanobacterial proteins. The repeats were first identified in hglK The function of these repeats is unknown. The structure of this repeat has been predicted to be a beta-helix . The repeat can be approximately described as A(D/N)LXX, where X can be any amino acid.; Conserved hypothetical protein; pentapeptide repeat-containing protein 3264388 4608690 azo2973 Azoarcus sp. BH72 pentapeptide repeat-containing protein YP_934476.1 3263828 D 62928 CDS YP_934477.1 119899264 4607718 3264394..3264876 1 NC_008702.1 Putative cytochrome c550. Homology to exaB of P. aeruginosa of 37% (trembl|Q9Z4P8). Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation. InterPro: Cytochrome c class I (IPR003088) Pfam: cytochrome c signal peptide no TMHs; Function unclear; putative cytochrome c550 3264876 exaB2 4607718 exaB2 Azoarcus sp. BH72 putative cytochrome c550 YP_934477.1 3264394 D 62928 CDS YP_934478.1 119899265 4608695 complement(3265163..3266950) 1 NC_008702.1 Quinoprotein ethanol dehydrogenase precursor (QEDH). Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol. 53% Bac_PQQ.IPR002372; Bac_PQQ_repeat. Pfam:PF01011; PQQ; 2. Signal peptide:present. TMhelix:1; High confidence in function and specificity; putative quinoprotein ethanol dehydrogenase 3266950 exaA3 4608695 exaA3 Azoarcus sp. BH72 putative quinoprotein ethanol dehydrogenase YP_934478.1 3265163 R 62928 CDS YP_934479.1 119899266 4608691 3267279..3268091 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2677 of P. putida of 35% (trembl|Q88JH2(SRS)). No domains predicted. Signal peptide present. No TMH detected.; Conserved hypothetical protein; hypothetical protein 3268091 4608691 azo2976 Azoarcus sp. BH72 hypothetical protein YP_934479.1 3267279 D 62928 CDS YP_934480.1 119899267 4607719 3268088..3269080 1 NC_008702.1 Conserved Hypothetical protein (pir:H95862; 47% identity ), putative hydrolase (TREMBL: Q88JH1; 54% identity, 66% similarity) putative cyclase (TREMBL: Q93IZ0) putative alkylsufatase/betalactamase (rba:RB11502) Metallo beta lactamase superfamily (Pfam) Signal P; TMHMM reporting transmembrane helices S18: ribosomal protein S18; High confidence in function and specificity; putative beta lactamase 3269080 cphA 4607719 cphA Azoarcus sp. BH72 putative beta lactamase YP_934480.1 3268088 D 62928 CDS YP_934481.1 119899268 4608528 3269103..3271517 1 NC_008702.1 Similar to FepA, an iron regulated outer membrane protein. FepA is a receptor for the siderophore complex ferric enterobactin and for colcins B and D. INVOLVED IN THE INITIAL STEP OF IRON UPTAKE BY BINDING FERRIC ENTEROBACTIN THAT ALLOWS E. COLI TO EXTRACT IRON FROM THE ENVIRONMENT. Probable tonB-dependent receptor yncD precursor. InterPro: TonB-dependent receptor protein.; High confidence in function and specificity; putative TonB-dependent receptor 3271517 4608528 azo2978 Azoarcus sp. BH72 putative TonB-dependent receptor YP_934481.1 3269103 D 62928 CDS YP_934482.1 119899269 4607720 3271523..3272992 1 NC_008702.1 Putative two-component sensor histidine kinase,; Specificity unclear; putative two-component sensor histidine kinase 3272992 elmS 4607720 elmS Azoarcus sp. BH72 putative two-component sensor histidine kinase YP_934482.1 3271523 D 62928 CDS YP_934483.1 119899270 4608665 3273206..3273850 1 NC_008702.1 Probable transcriptional regulator, LuxR family,; Specificity unclear; LuxR family transcriptional regulator 3273850 gacA 4608665 gacA Azoarcus sp. BH72 LuxR family transcriptional regulator YP_934483.1 3273206 D 62928 CDS YP_934484.1 119899271 4608853 3274283..3276310 1 NC_008702.1 Putative methyl-accepting chemotaxis protein,; Conserved hypothetical protein; putative methyl-accepting chemotaxis protein 3276310 4608853 azo2981 Azoarcus sp. BH72 putative methyl-accepting chemotaxis protein YP_934484.1 3274283 D 62928 CDS YP_934485.1 119899272 4607721 complement(3276637..3276912) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of M. magnetotacticum of 33% (ZP_00053175). No domains predicted. No TMHs. No signal peptide; hypothetical protein 3276912 4607721 azo2982 Azoarcus sp. BH72 hypothetical protein YP_934485.1 3276637 R 62928 CDS YP_934486.1 119899273 4607722 complement(3277196..3278320) 1 NC_008702.1 Putative Transposase,38% identity(55% similarity)to TrEMBL;Q877R1, Putative receptor protein (Transposase) [BT1893] [Bacteroides thetaiotaomicron VPI-5482]. Has PF01609:Transposase DDE domain;Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4 P03835, IS421 P11901, IS5377 Q45620, IS427 , IS402, IS1355 O69604, IS5,which was original isolated in bacteriophage lambda. IPR002559:Transposase_11. GO:0004872;Molecular function: receptor activity. Signal peptide present. NO TMH reported present.; Family membership; putative transposase 3278320 4607722 azo2983 Azoarcus sp. BH72 putative transposase YP_934486.1 3277196 R 62928 CDS YP_934487.1 119899274 4607723 3278702..3279538 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. Pfam: DUF1555. signal peptide. TMH in signal peptide; hypothetical protein 3279538 4607723 azo2984 Azoarcus sp. BH72 hypothetical protein YP_934487.1 3278702 D 62928 CDS YP_934488.1 119899275 4607724 3279788..3280681 1 NC_008702.1 Tankyrase 2 (EC 2.4.2.30) (TANK2) (Tankyrase II) (TNKS-2) (TRF1- interacting ankyrin-related ADP-ribose polymerase 2) (Tankyrase-like protein) (Tankyrase-related protein). May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TRF1 and thereby contribute to the regulation of telomere length. TREMBL:Q800E0:34%; Q8YTG9:36% identity. InterPro: Ankyrin-repeat Mop: molybdenum-pterin binding domain Presence of signal peptide (SignalP) but absence of transmembrane helices; Function unclear; putative tankyrase type protein 3280681 tnkS 4607724 tnkS Azoarcus sp. BH72 putative tankyrase type protein YP_934488.1 3279788 D 62928 CDS YP_934489.1 119899276 4609955 complement(3280842..3281492) 1 NC_008702.1 Similar to TREMBL:Q82SQ0 (50% identity ,67% similarity).; Function unclear; putative methyltransferase 3281492 4609955 azo2986 Azoarcus sp. BH72 putative methyltransferase YP_934489.1 3280842 R 62928 CDS YP_934490.1 119899277 4607725 complement(3281489..3282034) 1 NC_008702.1 Putative Dihydrofolate reductase.; Function unclear; dihydrofolate reductase 3282034 4607725 azo2987 Azoarcus sp. BH72 dihydrofolate reductase YP_934490.1 3281489 R 62928 CDS YP_934491.1 119899278 4607726 complement(3282330..3282890) 1 NC_008702.1 Probable chromate reductase. Homology to chrR of E. coli of 49% (tremblnew|AAK62985). Reduction of chromate (Cr(VI)) to Cr(III).no no signal peptide no TMHs; High confidence in function and specificity; chromate reductase 3282890 chrR 4607726 chrR Azoarcus sp. BH72 chromate reductase YP_934491.1 3282330 R 62928 CDS YP_934492.1 119899279 4608482 3283201..3285402 1 NC_008702.1 Peroxidase/catalase (EC 1.11.1.6). Bifunctional exhibiting both a catalase and broad- spectrum peroxidase activities.Peroxidases are haem-containing enzymes that use hydrogen peroxide as the electron acceptor to catalyse a number of oxidative reactions.Bacterial catalase-peroxidases are approximately twice the size of plant and fungal haem peroxidases. Sequence analysis suggests that this doubling in length can be ascribed to gene duplication, whereby each half is; High confidence in function and specificity; putative catalase/peroxidase 3285402 katG 4608482 katG Azoarcus sp. BH72 putative catalase/peroxidase YP_934492.1 3283201 D 62928 CDS YP_934493.1 119899280 4609099 complement(3285452..3286078) 1 NC_008702.1 Similar to TREMBL:Q88PY7 (90% identity); SWISSPROT:P21367 (87% identity); TREMBL:Q7W0U0 (85% identity). InterPro (IPR000868): Isochorismatase hydrolase. Pfam (PF00857): Isochorismatase family.; Specificity unclear; putative isochorismatase 3286078 4609099 azo2990 Azoarcus sp. BH72 putative isochorismatase YP_934493.1 3285452 R 62928 CDS YP_934494.1 119899281 4607727 complement(3286186..3287082) 1 NC_008702.1 Hypothetical protein PA2418. Pirin related protein of unknown function. TREMBL:Q7VUX8: 69% identity, 81% similarity. InterPro:IPR008778; Pirin_C. IPR003829; Pirin_N. Pfam: PF02678; Pirin; 1. PF05726; Pirin_C InterPro: DUF209 No signal peptide present (Signal P predicted). No transmembrane helices present (TMHMM predicted). hypD: hydrogenase expression/formation pr; Specificity unclear; hypothetical protein 3287082 4607727 azo2991 Azoarcus sp. BH72 hypothetical protein YP_934494.1 3286186 R 62928 CDS YP_934495.1 119899282 4607728 3287206..3288111 1 NC_008702.1 Probable transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 3288111 4607728 azo2992 Azoarcus sp. BH72 LysR family transcriptional regulator YP_934495.1 3287206 D 62928 CDS YP_934496.1 119899283 4607729 3288180..3288830 1 NC_008702.1 DNA-binding protein or Dihydrofolat-Reductase ???; Function unclear; DNA-binding protein 3288830 4607729 azo2993 Azoarcus sp. BH72 DNA-binding protein YP_934496.1 3288180 D 62928 CDS YP_934497.1 119899284 4607730 complement(3288843..3291056) 1 NC_008702.1 Iron-regulated outer membrane protein. TonB-dependent receptor protein. Homolog to fpvA, a ferripyoverdine receptor precursor in P. aeruginosa. Similar to PupA protein of P. putida WCS,this protein is a receptor for the iron-bound form of pesudobactin, a compound structurally very; High confidence in function and specificity; TonB-dependent receptor 3291056 fpvA2 4607730 fpvA2 Azoarcus sp. BH72 TonB-dependent receptor YP_934497.1 3288843 R 62928 CDS YP_934498.1 119899285 4608828 complement(3291403..3291858) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to IL2513 of Idiomarina loihiensis of 37% (gnl|keqq|ilo:IL2513(KEGG)). no domains predicted. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 3291858 4608828 azo2995 Azoarcus sp. BH72 hypothetical protein YP_934498.1 3291403 R 62928 CDS YP_934499.1 119899286 4607731 complement(3291855..3293027) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA2403 of Pseudomonas aeruginosa of 38% (trembl|Q8G8F6). Has 3 copies of PF03929, PepSY-associated TM helix;IPR005625 pEPsy_tm; This domain represents a conserved TM helix found in bacterial proteins. Has PF03413, Peptidase propeptide and YPEB domain; IPR005075,Propep_PepSY:This region is likely to have an protease inhibitory function (personal obs:C Yeats). This region is likely to have an protease inhibitory function (personal obs:C Yeats). This model is likely to miss some members of this family as the separation from signal to noise is not clear. The name is derived from Peptidase & Bacillus subtilis YPEB. no signal peptide. 4TMHs; Conserved hypothetical protein; hypothetical protein 3293027 4607731 azo2996 Azoarcus sp. BH72 hypothetical protein YP_934499.1 3291855 R 62928 CDS YP_934500.1 119899287 4607732 complement(3293030..3295177) 1 NC_008702.1 Ferrioxamine B receptor precursor, FhuF. Iron-regulated outer membrane protein that bind and uptake ferrioxamine in association with the TonB protein. Involved in the reduction of ferric iron in cytoplasmic ferrioxamine B. Binds 1 2Fe-2S cluster. In e.coli the fhuF gene reacts very sensitive to minor changes of Fe2+ and Fur(Ferric uptake regulator). SWISSPROT: FOXA_SALTY. Prim. accession #:Q56145 EMBL: AE008712; AAL19318.1; AF060876; AAC15464.1; U62282; AAB04552.1; InterPro:IPR000531; TonB_boxC. Pfam: PF00593; TonB_dep_Rec; 1. PROSITE: PS00430; TONB_DEPENDENT_REC_1; FALSE_NEG. PS01156; TONB_DEPENDENT_REC_2; FALSE_NEG.; High confidence in function and specificity; putative TonB-dependent receptor 3295177 fhuF 4607732 fhuF Azoarcus sp. BH72 putative TonB-dependent receptor YP_934500.1 3293030 R 62928 CDS YP_934501.1 119899288 4608762 3295639..3296769 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. InterPro: Esterase/lipase/thioesterase family active site (IPR000379), GCN5-realted N-actetyltransferase (IPR000182). no signal peptide. no TMHs; hypothetical protein 3296769 4608762 azo2998 Azoarcus sp. BH72 hypothetical protein YP_934501.1 3295639 D 62928 CDS YP_934502.1 119899289 4607733 complement(3296777..3297358) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NE2572 of N.europaea of 46% (tremble:Q82RZ3). Has PF05951, Bacterial protein of unknown function (DUF882);IPR010275; This family consists of a series of hypothetical bacterial proteins of unknown function. Signal Peptide Present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3297358 4607733 azo2999 Azoarcus sp. BH72 hypothetical protein YP_934502.1 3296777 R 62928 CDS YP_934503.1 119899290 4607734 complement(3297392..3299023) 1 NC_008702.1 Putative periplasmic protein [ycbB], 39% identity(57% similarity) to TrEMBL;Q82RZ2.Q9A853(30% identity) Signal Peptide Present. Has PF01471, Putative peptidoglycan binding domain; IPR002477 PG_binding; This domain, peptidoglycan binding domain 1, may have a general peptidoglycan binding function. It is composed of three helices and is found at the N or C terminus of a variety of enzymes involved in bacterial cell wall degradation. * Muramoyl-pentapeptide carboxypeptidase (EC:3.4.17.8) * N-acetylmuramoyl-L-alanine amidase cwlA precursor (cell wall) hydrolase, autolysin, EC:3.5.1.28) * Autolytic lysozyme (1,4--N-acetylmuramidase, autolysin, EC:3.2.1.17) * Membrane-bound lytic murein transglycosylase B; Family membership; hypothetical protein 3299023 ycbB 4607734 ycbB Azoarcus sp. BH72 hypothetical protein YP_934503.1 3297392 R 62928 CDS YP_934504.1 119899291 4610153 complement(3299083..3300033) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to XAC149 of X. axonopodis of 41% (trembl|Q8PLP4(SRS)). Pfam: DedA family. This family combines the DedA related proteins and YIAN/YGIK family. Members of this family are not functionally characterised. These proteins contain multiple predicted transmembrane regions. no signal peptide. 4 TMHs.; Conserved hypothetical protein; hypothetical protein 3300033 4610153 azo3001 Azoarcus sp. BH72 hypothetical protein YP_934504.1 3299083 R 62928 CDS YP_934505.1 119899292 4607735 complement(3300159..3300980) 1 NC_008702.1 Conserved hypothetical formylglutamte amindohyrolase. Homology to hutG of B. bronchiseptica of 53% (trembl|Q7WEA2). Catalyses the terminal reaction in the five-step pathway for histidine utilisation. no signal peptide. no TMHs; Conserved hypothetical protein; formylglutamte aminohyrolase 3300980 hutG 4607735 hutG Azoarcus sp. BH72 formylglutamte aminohyrolase YP_934505.1 3300159 R 62928 CDS YP_934506.1 119899293 4609054 complement(3300977..3302122) 1 NC_008702.1 Conserved hypothetical transcriptional regulator,LysR family. Similar to SWISSPROT: sprot|NTCB_SYNP7 (18% Synechococcus sp. (strain PCC 7942) (Anacystis nidulans R2), nitrogen assimilation transcriptional activator NtcB) Pfam: PF00126 Bacterial regulatory helix-turn-helix protein, lysR family. HTH reporting nucleic acid binding motif.; Family membership; hypothetical protein 3302122 4609054 azo3003 Azoarcus sp. BH72 hypothetical protein YP_934506.1 3300977 R 62928 CDS YP_934507.1 119899294 4607736 3302210..3303394 1 NC_008702.1 Sarcosine oxidase, subunit beta counts to the FAD dependent oxidoreductases. Similar to trembl|Q82M71 (29%) and to trembl|Q9V0K7 (26%). Pfam (PF01266): D-amino acid oxidase ProSite (PS50205): NAD binding site ProSite (PS50204): UBA/THIF-type NAD/FAD binding fold; Specificity unclear; putative sarcosine oxidase subunit beta 3303394 soxB 4607736 soxB Azoarcus sp. BH72 putative sarcosine oxidase subunit beta YP_934507.1 3302210 D 62928 CDS YP_934508.1 119899295 4609881 3303449..3304201 1 NC_008702.1 Probable electron transfer flavoprotein, beta subunit. Homology to etfB of B. japonicum of 54% (sprot|ETFB_BRAJA). The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein beta-subunit (IPR000049) Pfam: Electron transfer flavoprotein beta subunit no signal peptide no TMHS; High confidence in function and specificity; electron transfer flavoprotein subunit beta 3304201 etfB4 4609881 etfB4 Azoarcus sp. BH72 electron transfer flavoprotein subunit beta YP_934508.1 3303449 D 62928 CDS YP_934509.1 119899296 4608685 3304218..3305165 1 NC_008702.1 Probable electron transfer flavoprotein, alpha subunit. Homology to etfA of B. japonicum of 49% (sprot|ETFA_BRAJA) The electron transfer flavoprotein serves as a specific electron acceptor for some dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)(By similarity). InterPro: Electron transfer flavoprotein alpha-subunit (IPR001308) Pfam: Electron transfer flavoprotein alpha subunit no signal peptide no TMHs; High confidence in function and specificity; electron transfer flavoprotein subunit alpha 3305165 etfA4 4608685 etfA4 Azoarcus sp. BH72 electron transfer flavoprotein subunit alpha YP_934509.1 3304218 D 62928 CDS YP_934510.1 119899297 4608681 3305177..3305785 1 NC_008702.1 Conserved hypothetical protein. Homology to BB1278 of B.bronchiseptica of 35% (trembl:Q7WMW2). Has PF05962,Bacterial protein of unknown function (DUF886);IPR010282; This family consists of several hypothetical bacterial proteins of unknown function. No signal peptide or TMH present.; hypothetical protein 3305785 4608681 azo3007 Azoarcus sp. BH72 hypothetical protein YP_934510.1 3305177 D 62928 CDS YP_934511.1 119899298 4607737 3305802..3307487 1 NC_008702.1 Probable electron transfer flavoprotein-ubiquinone oxidoreductase. Homology to etfdh of H. sapiens of 53% (sprot|ETFD_HUMAN) Accepts electrons and reduces ubiquinone. Pfam: FAD_binding_3; ETF_QO no signal peptide no TMHs; High confidence in function and specificity; electron transfer flavoprotein-ubiquinone oxidoreductase 3307487 etf2 4607737 etf2 Azoarcus sp. BH72 electron transfer flavoprotein-ubiquinone oxidoreductase YP_934511.1 3305802 D 62928 CDS YP_934512.1 119899299 4608677 complement(3307684..3308286) 1 NC_008702.1 Putative DNA binding helix-turn helix protein,; Family membership; putative DNA binding helix-turn helix protein 3308286 4608677 azo3009 Azoarcus sp. BH72 putative DNA binding helix-turn helix protein YP_934512.1 3307684 R 62928 CDS YP_934513.1 119899300 4607738 3308451..3309848 1 NC_008702.1 Glutamine synthetase III plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. Similar to trembl|Q98A06 (58%) and to sprot|GLN3_RHILP (38%). Pfam (PF03951): Glutamine synthetase, beta-Grasp domain Pfam(PF00120): Glutamine synthetase, catalytic domain; High confidence in function and specificity; glutamine synthetase 3309848 glnT 4607738 glnT Azoarcus sp. BH72 glutamine synthetase YP_934513.1 3308451 D 62928 CDS YP_934514.1 119899301 4608905 3309864..3310772 1 NC_008702.1 Glutamine amidotransferase, class-II protein, glxB (purF). Similar to sprot|GLXB_RHIME (46%) and to trembl|Q882P3 (49%). Glutamine amidotransferase catalyse the removal of the ammonia group from glutamine and then to transfer this group to a substrate to form a new carbon-nitrogen group. This catalytic activity is known as glutamine amidotransferase (GATase). On the basis of sequence similarities two classes of GATase domains have been identified class-I and class-II (also known as purF-type). Enzymes containing Class-II GATase domains include amido phosphoribosyltransferase, which catalyses the first step in purine biosynthesis. InterPro (PF00310): Glutamine amidotransferase class-II; High confidence in function and specificity; amido phosphoribosyltransferase 3310772 glxB 4608905 glxB Azoarcus sp. BH72 amido phosphoribosyltransferase YP_934514.1 3309864 D 62928 CDS YP_934515.1 119899302 4608919 3310760..3311452 1 NC_008702.1 Probable protein GlxC. Similar to sprot|GLXC_RHIME (59%) and to pir|A96211 (51%). Pfam (PF01493): GXGXG; Family membership; protein GlxC 3311452 glxC 4608919 glxC Azoarcus sp. BH72 protein GlxC YP_934515.1 3310760 D 62928 CDS YP_934516.1 119899303 4608920 3311454..3312797 1 NC_008702.1 Glutamate synthase, large subunit. Similar to sprot|GLXD_RHIME (87%). Glutamate synthase (GltS) is a complex iron-sulphur flavoprotein that catalyses the reductive synthesis of L-glutamate from 2-oxoglutarate and L-glutamine via intramolecular channelling of ammonia, a reaction in the bacterial, yeast and plant pathways for ammonia assimilation. Pfam: Conserved region in glutamate synthase; Specificity unclear; glutamate synthase large subunit 3312797 glxD 4608920 glxD Azoarcus sp. BH72 glutamate synthase large subunit YP_934516.1 3311454 D 62928 CDS YP_934517.1 119899304 4608921 3312866..3313204 1 NC_008702.1 PII-like signal transmitter proteins are involved in the regulation of ammonium assimilation and nitrogen fixation. The PII-like proteins differed from each other in details of N-sensing. They were covalently modified by uridylylation upon nitrogen limitation. Similar to trembl|Q9EZQ4 (100%) and to trembl|Q9RBK0 (78%). Pfam: Nitrogen regulatory protein P-II; High confidence in function and specificity; PII-like signal transmitter protein GlnY 3313204 glnY 4608921 glnY Azoarcus sp. BH72 PII-like signal transmitter protein GlnY YP_934517.1 3312866 D 62928 CDS YP_934518.1 119899305 4608906 3313222..3314586 1 NC_008702.1 Ammonium transporter 1 member 2 (LeAMT1;2). Involved in high affinity ammonium uptake. TREMBL:Q8RP88: 100% identity (published) InterPro: IPR001905; Ammonium_transpt. IPR010256; RH_like_transpt. Pfam: PF00909; Ammonium_transp amt: ammonium transporter SignalP predicted signal peptide (probability 0.999) TMHMM predicted 11 transmembrane helices; High confidence in function and specificity; ammonium transporter 3314586 amtY 4608906 amtY Azoarcus sp. BH72 ammonium transporter YP_934518.1 3313222 D 62928 CDS YP_934519.1 119899306 4608319 complement(3314644..3315441) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ECA3203 of Erwinia carotovora of 58% (tremble:Q6D293). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3315441 4608319 azo3016 Azoarcus sp. BH72 hypothetical protein YP_934519.1 3314644 R 62928 CDS YP_934520.1 119899307 4607739 complement(3315684..3316808) 1 NC_008702.1 Outer membrane porin protein 32 precursor (OMP32). Forms anion selective channels.; Specificity unclear; outer membrane porin protein 3316808 4607739 azo3017 Azoarcus sp. BH72 outer membrane porin protein YP_934520.1 3315684 R 62928 CDS YP_934521.1 119899308 4607740 complement(3316976..3317098) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted .no signal peptide. no TMHs; hypothetical protein 3317098 4607740 azo3018 Azoarcus sp. BH72 hypothetical protein YP_934521.1 3316976 R 62928 CDS YP_934522.1 119899309 4607741 3317346..3318281 1 NC_008702.1 Putative AraC family,transcriptional regulator, 28% Identity to TrEMBL;Q62GH5,Q6QEJ0,Q9HWT4 Has SMART;SM00342,HTH_ARAC, helix_turn_helix, arabinose operon control protein;IPR000005; Many bacterial transcription regulation proteins bind DNA through a 'helix-turn-helix' (HTH) motif. One major subfamily of these proteins is related to the arabinose operon regulatory protein AraC. Except for celD all of these proteins seem to be positive transcriptional factors.Although the sequences belonging to this family differ somewhat in length, in nearly every case the HTH motif is situated towards the C-terminus in the third quarter of most of the sequences. The minimal DNA binding domain spans roughly 100 residues and comprises two HTH subdomains; the classical HTH domain and another HTH subdomain with similarity to the classical HTH domain but with an insertion of one residue in the turn-region. The N-terminal and central regions of these proteins are presumed to interact with effector molecules and may be involved in dimerization.; Conserved hypothetical protein; putative AraC-like transcriptional regulator 3318281 4607741 azo3019 Azoarcus sp. BH72 putative AraC-like transcriptional regulator YP_934522.1 3317346 D 62928 CDS YP_934523.1 119899310 4607742 3318463..3319443 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to aciad2673 of Acinetobacter sp. of 53% (trembl|Q6F937). no domains reported. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 3319443 4607742 azo3020 Azoarcus sp. BH72 hypothetical protein YP_934523.1 3318463 D 62928 CDS YP_934524.1 119899311 4607743 3319525..3320985 1 NC_008702.1 Probable succinate semialdehyde dehydrogenase [NAD(P)+]. Homology to thmS of Pseudonocardia sp. K1 of 46% (trembl|Q9F3V7). Is capable of oxidizing substrates using NADP as cofactor. Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; Family membership; succinate semialdehyde dehydrogenase 3320985 thmS2 4607743 thmS2 Azoarcus sp. BH72 succinate semialdehyde dehydrogenase YP_934524.1 3319525 D 62928 CDS YP_934525.1 119899312 4609942 3321256..3323430 1 NC_008702.1 Quinoprotein ethanol dehydrogenase precursor (QEDH). Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol. 37% Bac_PQQ.IPR002372; Bac_PQQ_repeat. Pfam:PF01011; PQQ; 2. Signal peptide:present.; High confidence in function and specificity; putative quinoprotein ethanol dehydrogenase 3323430 exaA4 4609942 exaA4 Azoarcus sp. BH72 putative quinoprotein ethanol dehydrogenase YP_934525.1 3321256 D 62928 CDS YP_934526.1 119899313 4608692 3323986..3326103 1 NC_008702.1 Probable outer membrane receptor TonB-dependent for iron transport. 41% AAC74533.1 InterPro: IPR000531 TonB_receptor.IPR010105; TonB_siderophor. Pfam: PF00593; TonB_dep_Rec; 1. TonB-box domain; High confidence in function and specificity; TonB-dependent receptor 3326103 4608692 azo3023 Azoarcus sp. BH72 TonB-dependent receptor YP_934526.1 3323986 D 62928 CDS YP_934527.1 119899314 4607744 3326109..3326648 1 NC_008702.1 Hypotheical secreted protein. No homology of the entire protein to the data bank. no domains predicted. no TMHs. signal peptide.; hypothetical protein 3326648 4607744 azo3024 Azoarcus sp. BH72 hypothetical protein YP_934527.1 3326109 D 62928 CDS YP_934528.1 119899315 4607745 3326814..3327098 1 NC_008702.1 Conserved hypothetical protein. Homology to ra0176 of S. meliloti of 31% (trembl|Q930L9). Pfam: Uncharacterized ACR, YneC family. no signal peptide. no TMHS; hypothetical protein 3327098 4607745 azo3025 Azoarcus sp. BH72 hypothetical protein YP_934528.1 3326814 D 62928 CDS YP_934529.1 119899316 4607746 3327420..3329078 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Reut02004453 of Ralstonia metallidurans of 38% (gi|48768314|ref|ZP_00272664.1|(NBCI ENTREZ)). no domains predicted. signal peptide. no TMHs.; Conserved hypothetical protein; hypothetical protein 3329078 4607746 azo3026 Azoarcus sp. BH72 hypothetical protein YP_934529.1 3327420 D 62928 CDS YP_934530.1 119899317 4607747 3329161..3329490 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS04458 of Ralstonia solanacearum of 31% (tremble:Q8Y292). No domains predicted .Signal peptide reported. NO TMH present.; Conserved hypothetical protein; hypothetical protein 3329490 4607747 azo3027 Azoarcus sp. BH72 hypothetical protein YP_934530.1 3329161 D 62928 CDS YP_934531.1 119899318 4607748 3329491..3330198 1 NC_008702.1 Conserved hypothetical peptidase. Homology to rsc0445 of R. solanacearum of 50% (trembl|Q8Y291). Pfam: Peptidas_C39. Lantibiotic and non-lantibiotic bacteriocins are synthesised as precursor peptides containing N-terminal extensions (leader peptides) which are cleaved off during maturation. Most non-lantibiotics and also some lantibiotics have leader peptides of the so-called double-glycine type. These leader peptides share consensus sequences and also a common processing site with two conserved glycine residues in positions -1 and -2. The double- glycine-type leader peptides are unrelated to the N-terminal signal sequences which direct proteins across the cytoplasmic membrane via the sec pathway. Their processing sites are also different from typical signal peptidase cleavage sites, suggesting that a different processing enzyme is involved. signal peptide. no TMHs; Conserved hypothetical protein; peptidase 3330198 4607748 azo3028 Azoarcus sp. BH72 peptidase YP_934531.1 3329491 D 62928 CDS YP_934532.1 119899319 4607749 3330212..3331177 1 NC_008702.1 Hypothetical secreted protein. No good homology over the entire length in DB. No domains predicted. Signal Peptide present. No TMH present.; hypothetical protein 3331177 4607749 azo3029 Azoarcus sp. BH72 hypothetical protein YP_934532.1 3330212 D 62928 CDS YP_934533.1 119899320 4607750 complement(3331211..3332539) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS04455 of R.solanacearum of 50% (tremble:Q8Y289). No domains predicted. Signal P reporting signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 3332539 4607750 azo3030 Azoarcus sp. BH72 hypothetical protein YP_934533.1 3331211 R 62928 CDS YP_934534.1 119899321 4607751 complement(3332594..3333922) 1 NC_008702.1 Probable transcriptional regulatory protein,; Specificity unclear; transcriptional regulator 3333922 4607751 azo3031 Azoarcus sp. BH72 transcriptional regulator YP_934534.1 3332594 R 62928 CDS YP_934535.1 119899322 4607752 3334477..3334860 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. TMH in signal peptide.; hypothetical protein 3334860 4607752 azo3032 Azoarcus sp. BH72 hypothetical protein YP_934535.1 3334477 D 62928 CDS YP_934536.1 119899323 4607753 3334857..3334991 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3334991 4607753 azo3033 Azoarcus sp. BH72 hypothetical protein YP_934536.1 3334857 D 62928 CDS YP_934537.1 119899324 4607754 3335002..3335409 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted. no signal peptide. 3 TMHs; hypothetical protein 3335409 4607754 azo3034 Azoarcus sp. BH72 hypothetical protein YP_934537.1 3335002 D 62928 CDS YP_934538.1 119899325 4607755 3335418..3336713 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank. no domains predicted. signal peptide. 2 TMHs; hypothetical protein 3336713 4607755 azo3035 Azoarcus sp. BH72 hypothetical protein YP_934538.1 3335418 D 62928 CDS YP_934539.1 119899326 4607756 complement(3336852..3337970) 1 NC_008702.1 Conserved hypothetical protein. Homology to Rgel02002356 of Rubrivivax gelatinosus of 47% (gi|47573226|ref|ZP_00243265.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 3337970 4607756 azo3036 Azoarcus sp. BH72 hypothetical protein YP_934539.1 3336852 R 62928 CDS YP_934540.1 119899327 4607757 complement(3337939..3338643) 1 NC_008702.1 conserved hypothetical protein. Homology to Rgel02002355 of Rubrivivax gelatinosus of 41% (gi|47573225|ref|ZP_00243264.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 3338643 4607757 azo3037 Azoarcus sp. BH72 hypothetical protein YP_934540.1 3337939 R 62928 CDS YP_934541.1 119899328 4607758 complement(3339274..3342159) 1 NC_008702.1 Probable formate dehydrogenase (NADP+), alpha subunit. Homology to fdh1A of M. extorquens of 60% (trembl|Q8KTI7). InterPro: Prokaryotic molybdopterin oxidoreductases (IPR006655); Respiratory-chain NADH dehydrogenase 75 kD subunit (IPR000283); Ferredoxin (IPR001041); 4Fe-4S ferredoxin, iron-sulfur binding domain (IPR001450) Pfam: 2Fe-2S iron-sulfur cluster binding domain; 4Fe-2S binding doami; Molybdopterin oxidase; Molybdopterin dinucloetide binding no signal peptide no TMHs; High confidence in function and specificity; formate dehydrogenase subunit alpha 3342159 fdhA1 4607758 fdhA1 Azoarcus sp. BH72 formate dehydrogenase subunit alpha YP_934541.1 3339274 R 62928 CDS YP_934542.1 119899329 4608740 complement(3342156..3343871) 1 NC_008702.1 Probable formate dehydrogenase (NADP+), beta subunit Homology to fdh1B of M. extorpuens of 55% (trembl|Q8KTI8) Pfam: Respiratory-chain NADH dehydrogenase 24 kD subunit; Respiratory-chain NADH dehydrogenase 51 kD subunit. no signal peptide no TMHs; High confidence in function and specificity; formate dehydrogenase (NADP+) subunit beta 3343871 fdh1B 4608740 fdh1B Azoarcus sp. BH72 formate dehydrogenase (NADP+) subunit beta YP_934542.1 3342156 R 62928 CDS YP_934543.1 119899330 4608739 complement(3344244..3345770) 1 NC_008702.1 converts 3-hydroxyadipyl-CoA to beta-ketoadipyl-CoA in phenylacetate degradation; 3-hydroxyacyl-CoA dehydrogenase 3345770 paaH2 4608739 paaH2 Azoarcus sp. BH72 3-hydroxyacyl-CoA dehydrogenase YP_934543.1 3344244 R 62928 CDS YP_934544.1 119899331 4609479 complement(3345835..3346002) 1 NC_008702.1 Probable rubredoxin. Homology to ruba of A. calcoaceticus of 42% (sprot|RUBR_ACIAD). Involved in the hydrocarbon hydroxylating system to convert dodecane to lauric acid, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase. Pfam: Rubredoxin no singal peptide no TMHs; Family membership; rubredoxin 3346002 rubA 4609479 rubA Azoarcus sp. BH72 rubredoxin YP_934544.1 3345835 R 62928 CDS YP_934545.1 119899332 4609832 complement(3346059..3347039) 1 NC_008702.1 ZP_00167050: 52% identity; 70% similarity. Quinone oxidoreductase (EC 1.6.5.5) (NADPH:quinone reductase) (Zeta- crystallin). DOES NOT HAVE ALCOHOL DEHYDROGENASE ACTIVITY. BINDS NADP AND ACTS THROUGH A ONE-ELECTRON TRANSFER PROCESS. ORTHOQUINONES ARE THE BEST SUBSTRATES. MAY ACT IN THE DETOXIFICATION OF XENOBIOTICS (BY SIMILARITY). InterPro: Zinc-containing alcohol dehydrogenase superfamily InterPro:IPR002085; Adh_zn_family. Pfam:PF00107; ADH_zinc_N TIGRFAM: 2A0302: arginine/ornithine antiporter tdh: L-threonine 3-dehydrogenase; Function unclear; NADPH quinone oxidoreductase 3347039 4609832 azo3042 Azoarcus sp. BH72 NADPH quinone oxidoreductase YP_934545.1 3346059 R 62928 CDS YP_934546.1 119899333 4607759 complement(3347056..3347859) 1 NC_008702.1 COULD POSSIBLY OXIDIZES FATTY ACIDS USING SPECIFIC COMPONENTS (BY SIMILARITY). catalytic activity:-(3s)-3-hydroxyacyl-coa = trans-2(or 3)-enoyl- coa + H(2)o. Entry name:- SWISSPROT:PAAG_ECOLI InterPro:- IPR001753; EnCoA_hydrtse. Pfam:- PF00378; ECH; 1. Identities = 72/262 (27%) Number of predicted TMHs: 0; Family membership; enoyl-CoA hydratase 3347859 paaG4 4607759 paaG4 Azoarcus sp. BH72 enoyl-CoA hydratase YP_934546.1 3347056 R 62928 CDS YP_934547.1 119899334 4609475 complement(3347868..3348293) 1 NC_008702.1 Hypothetical protein HI0386. TREMBL:Q84HI6: 48% identity, 66% similarity 4-hydroxybenzoyl-CoA thioesterase is a protein of 141 amino-acid residues that assemble as an homotetramer. An aspartate in the N-terminal domain is thought to participate in the catalytic mechanism 4-hydroxybenzoyl-CoA + H2 O = 4-hydroxybenzoate + CoA IPR006683; Thioesterase superfamily IPR006684; 4-hydroxybenzoyl-CoA thioesterase Pfam:PF03061; 4HBT; No transmembrane helices (TMHMM predicted) TIGR00051: conserved hypothetical protein; High confidence in function and specificity; thioesterase 3348293 ybgC 4609475 ybgC Azoarcus sp. BH72 thioesterase YP_934547.1 3347868 R 62928 CDS YP_934548.1 119899335 4610148 complement(3348797..3349570) 1 NC_008702.1 TREMBL:Q84HI5: 83% identity, 89% similarity from Azoarcus evansii (gene name refereed as ORF2) Haloalkane dehalogenase (EC 3.8.1.5). Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds leading to the formation of the corresponding primary alcohols halide ions and protons. Has a broad substrate specificity which includes mono- and di- chlorinated and brominated alkanes. The highest activity was found with 12-dibromoethane whereas low activity was measured with the analog 12-dichloroethane. InterPro: Alpha/beta hydrolase fold Description:lactonase. InterPro:IPR000379; Ser_estrs Pfam:abhydrolase_2, Thioesterase aroC: chorismate synthase Signal P predicted nonsecretiory protein without transmembrane helices.; High confidence in function and specificity; lactonase 3349570 4610148 azo3045 Azoarcus sp. BH72 lactonase YP_934548.1 3348797 R 62928 CDS YP_934549.1 119899336 4607760 complement(3349583..3350377) 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q84HI4 (80%) and to trembl|Q89VH8 (45%). Pfam (PF00005): ABC transporter Smart (SM00382): AAA ATPase superfamily ProSite (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; ABC transporter ATP-binding protein 3350377 4607760 azo3046 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934549.1 3349583 R 62928 CDS YP_934550.1 119899337 4607761 complement(3350370..3351176) 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q84HI3 (79%) and to sprot|LIVG_ECOLI (37%). Pfam (PF00005): ABC transporter ProSite (PS50101): ATP/GTP-binding site motif A (P-loop) Smart (SM00382): AAA ATPase superfamily; Specificity unclear; ABC transporter ATP-binding protein 3351176 livG1 4607761 livG1 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934550.1 3350370 R 62928 CDS YP_934551.1 119899338 4609149 complement(3351169..3352149) 1 NC_008702.1 Putative branched-chain amino acid transport permease. Homology to livM of E. coli of 31% as well as genes coding for a new pathway of aerobic benzoate meabolisme in A. evansii (77%). Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. Pfam: Branched-chain amino acid transport system. probable signal peptide probable 8 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 3352149 livM3 4609149 livM3 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_934551.1 3351169 R 62928 CDS YP_934552.1 119899339 4609160 complement(3352156..3353022) 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q84HI2 (91%), to trembl|Q89VI1 (47%), to sprot|LIVH_ECOLI (27%) and to sprot|BRAD_PSEAE (30%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting six Tmhelix.; Specificity unclear; ABC transporter permease 3353022 4609160 azo3049 Azoarcus sp. BH72 ABC transporter permease YP_934552.1 3352156 R 62928 CDS YP_934553.1 119899340 4607762 complement(3353237..3354418) 1 NC_008702.1 In enteric bacteria such E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar trembl|Q93FB9 (84%) and to sprot|LIVJ_ECOLI (21%). Pfam (PF01094): Receptor family ligand binding region Pfam (PF04392): Protein of unknown function (DUF534) SignalP reporting Signal peptide.; Specificity unclear; putative leucine-specific binding protein 3354418 livJ 4607762 livJ Azoarcus sp. BH72 putative leucine-specific binding protein YP_934553.1 3353237 R 62928 CDS YP_934554.1 119899341 4609152 complement(3354486..3354983) 1 NC_008702.1 Conserved hypothetical protein. Homology Daro03002253 of Dechloromonas aromatica of 43% (gi|53730397|ref|ZP_00151095.2|(NBCI ENTREZ)). Pfam: Hemerythrin HHE cation binding domain. Iteration of the HHE family found it to be related to Hemerythrin. It also demonstrated that what has been described as a single domain in fact consists of two cation binding domains. Members of this family occur all across nature and are involved in a variety of processes. For instance, in Nereis diversicolor MP2_NERDI binds Cadmium so as to protect the organism from toxicity. However Hemerythrin is classically described as Oxygen-binding through two attached Fe2+ ions. And the bacterial Q7WX96_ALCEU is a regulator of response to NO, which suggests yet another set-up for its metal ligands. In Staphylococcus aureus P72360 has been noted to be important when the organism switches to living in environments with low oxygen concentrations; perhaps this protein acts as an oxygen store or scavenger. No TMHs. No signal peptide.; hypothetical protein 3354983 4609152 azo3051 Azoarcus sp. BH72 hypothetical protein YP_934554.1 3354486 R 62928 CDS YP_934555.1 119899342 4607763 complement(3354997..3356595) 1 NC_008702.1 Entry name :- TREMBL:Q84HI0 Entry name :-TREMBL:Q8VUF1 InterPro IPR010071; AA_adenyl_dom. IPR000873; AMP-bind. IPR010192; MenE. Pfam PF00501; AMP-binding; 1 Identities = 414/532 (77%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; benzoate-coa ligase 3356595 bzdA 4607763 bzdA Azoarcus sp. BH72 benzoate-coa ligase YP_934555.1 3354997 R 62928 CDS YP_934556.1 119899343 4608408 complement(3356689..3357153) 1 NC_008702.1 Conserved hypothetical protein. Homology to an orf of A. evnasii of 79% (trembl|Q84HH9). no domains predicted. no signal peptide. no TMHS; hypothetical protein 3357153 4608408 azo3053 Azoarcus sp. BH72 hypothetical protein YP_934556.1 3356689 R 62928 CDS YP_934557.1 119899344 4607764 complement(3357353..3358930) 1 NC_008702.1 Aldehyde dehydrogenase (NAD+). Homology to the aldehyce dehydrogenase of the of the benzoate gene cluster of A. evansii of 81% (trembl|Q84HH8). Aldehyde dehydrogenases are enzymes which oxidize a wide variety of aliphatic and aromatic aldehydes using NADP as a cofactor. Here clustered with genes involved in the aerobic benzoate metabolism. InterPro: Aldehyde dehydrogenase family (IPR002086) Pfam: Aldehyde dehydrogenase family no signal peptide no TMHs; High confidence in function and specificity; aldehyde dehydrogenase 3358930 4607764 azo3054 Azoarcus sp. BH72 aldehyde dehydrogenase YP_934557.1 3357353 R 62928 CDS YP_934558.1 119899345 4607765 3359261..3360175 1 NC_008702.1 consists of N-terminal helix-turn-helix domain and C-terminal shikimate kinase-like domain which may bind benzoyl-CoA; controls inducible expression of the bzd catabolic operon that is involved in the anaerobic catabolism of benzoate; anaerobic benzoate catabolism transcriptional regulator 3360175 4607765 azo3055 Azoarcus sp. BH72 anaerobic benzoate catabolism transcriptional regulator YP_934558.1 3359261 D 62928 CDS YP_934559.1 119899346 4607766 3360200..3361864 1 NC_008702.1 cleaves the ring of 2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming 6-hydroxy-3-hexenoyl-CoA; benzoyl-CoA-dihydrodiol lyase 3361864 4607766 azo3056 Azoarcus sp. BH72 benzoyl-CoA-dihydrodiol lyase YP_934559.1 3360200 D 62928 CDS YP_934560.1 119899347 4607767 3362066..3363490 1 NC_008702.1 Benzoyl-CoA oxygenase component B. 85%; Function unclear; benzoyl-CoA oxygenase component B 3363490 boxB 4607767 boxB Azoarcus sp. BH72 benzoyl-CoA oxygenase component B YP_934560.1 3362066 D 62928 CDS YP_934561.1 119899348 4608402 3363690..3364940 1 NC_008702.1 Benzoyl-CoA oxygenase component A. 84% 4Fe4S_ferredoxin. IPR001709; FPN_cyt_redctse. IPR001433; Oxred_FAD/NAD(P). Pfam:PF00037; Fer4; 2. PF00175; NAD_binding_1; 1.; High confidence in function and specificity; putative benzoyl-CoA oxygenase component A 3364940 boxA 4608402 boxA Azoarcus sp. BH72 putative benzoyl-CoA oxygenase component A YP_934561.1 3363690 D 62928 CDS YP_934562.1 119899349 4608401 3365275..3366483 1 NC_008702.1 catalyzes the thiolytic cleavage of beta-ketoadipyl-CoA to succinate and acetyl-CoA; beta-ketoadipyl CoA thiolase 3366483 4608401 azo3059 Azoarcus sp. BH72 beta-ketoadipyl CoA thiolase YP_934562.1 3365275 D 62928 CDS YP_934563.1 119899350 4607768 3366498..3367259 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA2769 of Azoarcus sp. EbN1 of 56% (gnl|keqq|eba:ebA2769(KEGG)). Pfam: Hemerythrin HHE cation binding domain. Iteration of the HHE family found it to be related to Hemerythrin. It also demonstrated that what has been described as a single domain in fact consists of two cation binding domains. Members of this family occur all across nature and are involved in a variety of processes. For instance, in Nereis diversicolor MP2_NERDI binds Cadmium so as to protect the organism from toxicity. However Hemerythrin is classically described as Oxygen-binding through two attached Fe2+ ions. And the bacterial Q7WX96_ALCEU is a regulator of response to NO, which suggests yet another set-up for its metal ligands. In Staphylococcus aureus P72360 has been noted to be important when the organism switches to living in environments with low oxygen concentrations; perhaps this protein acts as an oxygen store or scavenger. No TMHs. No signal peptide.; hypothetical protein 3367259 4607768 azo3060 Azoarcus sp. BH72 hypothetical protein YP_934563.1 3366498 D 62928 CDS YP_934564.1 119899351 4607769 complement(3367800..3370820) 1 NC_008702.1 GGDEF/EAL/PAS/PAC/GAF-domain containing protein; diguanylate cyclase 3370820 4607769 azo3061 Azoarcus sp. BH72 diguanylate cyclase YP_934564.1 3367800 R 62928 CDS YP_934565.1 119899352 4607770 complement(3371066..3372010) 1 NC_008702.1 Putative Ribonuclease RNASE_BN Protein, 28% identity to TrEMBL;Q8XPH6. Has PF03631, Ribonuclease BN-like family;IPR004664, RNase_BN: This family contains integral membrane proteins with 5 to 6 predicted transmembrane spans. The family include ribonuclease BN that is involved in tRNA maturation P32146. This family of proteins does not seem to contain any completely conserved polar residues that would be expected in a nuclease enzyme, suggesting that many members of this family may not have this catalytic activity.; Conserved hypothetical protein; exoribonuclease II 3372010 brkB 4607770 brkB Azoarcus sp. BH72 exoribonuclease II YP_934565.1 3371066 R 62928 CDS YP_934566.1 119899353 4608406 complement(3372179..3373117) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV2531 of C. violaceum of 37%. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Tranporter. Pfam: Integral membrane protein DUF6. probable 10 TMHs. probable signal peptide; Conserved hypothetical protein; hypothetical protein 3373117 4608406 azo3063 Azoarcus sp. BH72 hypothetical protein YP_934566.1 3372179 R 62928 CDS YP_934567.1 119899354 4607771 3373359..3373826 1 NC_008702.1 Leucine-responsive regulatory protein. Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different opreons; Lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes. Similar to SWISSPROT: sprot|LRP_ECOLI (40% Escherichia coli, leucine-responsive regulatory protein Lrp) InterPro: IPR000485 HTH_AsnC_lrp. Pfam: PF01037 AsnC family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; leucine-responsive regulatory protein 3373826 lrp1 4607771 lrp1 Azoarcus sp. BH72 leucine-responsive regulatory protein YP_934567.1 3373359 D 62928 CDS YP_934568.1 119899355 4609180 complement(3374096..3375016) 1 NC_008702.1 Hydroxymethylglutaryl-CoA lyase, MvaB. It catalyses the conversion of (S)-3-hydroxy-3-methylglutaryl-CoA to acetyl-CoA and acetoacetate during the final step of ketogenesis and leucine catabolism. Similar to trembl|Q8QGJ4 (60%) and to pir|H83394 (56%). InterPro (PF00682): HMG-CoA Lyase-like family; High confidence in function and specificity; hydroxymethylglutaryl-CoA lyase 3375016 mvaB 4609180 mvaB Azoarcus sp. BH72 hydroxymethylglutaryl-CoA lyase YP_934568.1 3374096 R 62928 CDS YP_934569.1 119899356 4609299 complement(3375027..3376715) 1 NC_008702.1 activates fatty acids by binding to coenzyme A; AMP-binding protein 3376715 fadD3 4609299 fadD3 Azoarcus sp. BH72 AMP-binding protein YP_934569.1 3375027 R 62928 CDS YP_934570.1 119899357 4608724 complement(3376848..3377219) 1 NC_008702.1 Hypothetical protein ycjD,50% identity(65% similarity) to SwissProt;P45736. Has PF04480, Protein of unknown function (DUF559);IPR007569; No SIgnal Peptide or TMH present. vsr: DNA mismatch endonuclease (vsr); hypothetical protein 3377219 ycjD 4608724 ycjD Azoarcus sp. BH72 hypothetical protein YP_934570.1 3376848 R 62928 CDS YP_934571.1 119899358 4610162 complement(3377487..3379487) 1 NC_008702.1 This protein is a component of the acetyl coenzyme a carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-coa. Entry name TREMBL:Q8PDU1 InterPro IPR001882; Biotin_BS. IPR005482; Biotin_carb_C. IPR000089; Biotin_lipoyl. IPR005479; CPase_L_D2. IPR005481; CPase_L_N. Pfam PF02785; Biotin_carb_C; 1. PF00364; Biotin_lipoyl; 1. PF00289; CPSase_L_chain; 1. PF02786; CPSase_L_D2; 1. Identities = 382/661 (57%) Preediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; biotin carboxylase 3379487 accC2 4610162 accC2 Azoarcus sp. BH72 biotin carboxylase YP_934571.1 3377487 R 62928 CDS YP_934572.1 119899359 4608257 complement(3379877..3380665) 1 NC_008702.1 Catalyzes the reversible hydration of unsaturated fatty acyl-CoA to beta-hydroxyacyl-CoA; enoyl-CoA hydratase 3380665 paaG5 4608257 paaG5 Azoarcus sp. BH72 enoyl-CoA hydratase YP_934572.1 3379877 R 62928 CDS YP_934573.1 119899360 4609476 complement(3380677..3382284) 1 NC_008702.1 Activity:- ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA Entry name:-TREMBL:Q7WDP0 InterPro:- IPR000022; Carboxyl_trans. Pfam:-PF01039; Carboxyl_trans; 1. Identities = 413/535 (77%) Number of predicted TMHs: 0 Prediction: Non-secretory protein Signal peptide probability: 0.098; Family membership; carboxyltransferase subunit of acetyl-CoA carboxylase 3382284 4609476 azo3070 Azoarcus sp. BH72 carboxyltransferase subunit of acetyl-CoA carboxylase YP_934573.1 3380677 R 62928 CDS YP_934574.1 119899361 4607772 complement(3382372..3382614) 1 NC_008702.1 Region start changed from 3382650 to 3382614 (-36 bases), , Changed start codon from att to next gtg; hypothetical protein 3382614 4607772 azo3071 Azoarcus sp. BH72 hypothetical protein YP_934574.1 3382372 R 62928 CDS YP_934575.1 119899362 4607773 complement(3382666..3383205) 1 NC_008702.1 hypothetical protein 3383205 4607773 azo3072 Azoarcus sp. BH72 hypothetical protein YP_934575.1 3382666 R 62928 CDS YP_934576.1 119899363 4607774 complement(3383307..3384761) 1 NC_008702.1 Hypothetical protein. No homology to the data base. Pfam: Protein tyrosine kinase, Protein kinase domain. Interpro: IPR000719 Protein kinase, IPR002290 Serine/threonine protein kinase, IPR001245 Tyrosine protein kinase, IPR011009 Protein kinase-like. No signal peptide. No TMHs; hypothetical protein 3384761 4607774 azo3073 Azoarcus sp. BH72 hypothetical protein YP_934576.1 3383307 R 62928 CDS YP_934577.1 119899364 4607775 complement(3384762..3385667) 1 NC_008702.1 hypothetical protein 3385667 4607775 azo3074 Azoarcus sp. BH72 hypothetical protein YP_934577.1 3384762 R 62928 CDS YP_934578.1 119899365 4607776 complement(3385767..3386345) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. signal peptide. 3 TMHs; hypothetical protein 3386345 4607776 azo3075 Azoarcus sp. BH72 hypothetical protein YP_934578.1 3385767 R 62928 CDS YP_934579.1 119899366 4607777 3387069..3387359 1 NC_008702.1 Conserved hypothetical protein. Homology to TdenA01001814 of Thiobacillus denitrificans of 40% (gi|52006871|ref|ZP_00334250.1|(NBCI ENTREZ)). Has PF01402, Ribbon-helix-helix protein, copG family; IPR002145 HTH_CopG; The structure of this protein repressor, which is the shortest reported to date and the first isolated from a plasmid, has a homodimeric ribbon-helix-helix arrangement. The helix-turn-helix-like structure is involved in dimerisation and not DNA binding as might have been expected. No TMHs. No signal peptide.; hypothetical protein 3387359 4607777 azo3076 Azoarcus sp. BH72 hypothetical protein YP_934579.1 3387069 D 62928 CDS YP_934580.1 119899367 4607778 3387356..3387649 1 NC_008702.1 Conserved hypothetical protein. Homology to xcc3092 of X. campestris of 51% (trembl|Q8P678). Pfam: Plasmid stabilisation system protein. Members of this family are involved in plasmid stabilisation. The exact molecular function of this protein is not known. no signal peptide. no TMHs; hypothetical protein 3387649 4607778 azo3077 Azoarcus sp. BH72 hypothetical protein YP_934580.1 3387356 D 62928 CDS YP_934581.1 119899368 4607779 complement(3387664..3388353) 1 NC_008702.1 Putative transcriptional regulator, deoR-family; DeoR family transcriptional regulator 3388353 4607779 azo3078 Azoarcus sp. BH72 DeoR family transcriptional regulator YP_934581.1 3387664 R 62928 CDS YP_934582.1 119899369 4607780 3388508..3388876 1 NC_008702.1 Conserved hypothetical protein. Homology to stm0409 of S. typhimurium of 46% (tremble:Q7CR38). InterPro: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily. Pfam: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily. no signal peptide. no TMHs.; hypothetical protein 3388876 4607780 azo3079 Azoarcus sp. BH72 hypothetical protein YP_934582.1 3388508 D 62928 CDS YP_934583.1 119899370 4607781 3389559..3389756 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 3389756 4607781 azo3080 Azoarcus sp. BH72 hypothetical protein YP_934583.1 3389559 D 62928 CDS YP_934584.1 119899371 4607782 complement(3389852..3391024) 1 NC_008702.1 Entry name:- TREMBL:Q89LX4 Identities = 310/374 (82%) InterPro IPR006089; Acyl-CoA_dh. IPR006090; Acyl-CoA_dh_C. IPR006091; Acyl-CoA_dh_M. IPR006092; Acyl-CoA_dh_N. IPR009075; AcylCoADH_C_like. IPR009100; AcylCoA_dehyd_NM. Pfam PF00441; Acyl-CoA_dh; 1. PF02770; Acyl-CoA_dh_M; 1. PF02771; Acyl-CoA_dh_N; Family membership; acyl-coa dehydrogenase 3391024 4607782 azo3081 Azoarcus sp. BH72 acyl-coa dehydrogenase YP_934584.1 3389852 R 62928 CDS YP_934585.1 119899372 4607783 3391123..3392256 1 NC_008702.1 Putative ornithine utilization regulator,; Specificity unclear; putative ornithine utilization regulator 3392256 oruR2 4607783 oruR2 Azoarcus sp. BH72 putative ornithine utilization regulator YP_934585.1 3391123 D 62928 CDS YP_934586.1 119899373 4609459 complement(3392619..3393011) 1 NC_008702.1 Conserved hypothetical membrane protein, 44% identity to TrEMBL;Q82UI6. Has PF04247;Invasion gene expression up-regulator, SirB;IPR007360;SirB up-regulates Salmonella typhimurium invasion gene transcription. It is,however, not essential for the expression of these genes. Its function is unknown. No signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 3393011 4609459 azo3083 Azoarcus sp. BH72 hypothetical protein YP_934586.1 3392619 R 62928 CDS YP_934587.1 119899374 4607784 3393105..3393806 1 NC_008702.1 Putative transcriptional regulator,; Family membership; putative transcriptional regulator 3393806 flp 4607784 flp Azoarcus sp. BH72 putative transcriptional regulator YP_934587.1 3393105 D 62928 CDS YP_934588.1 119899375 4608814 complement(3393825..3395108) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV2709 of Chromobacterium violaceum of 40% (trembl|Q7NUI9). Has PF04550;Phage holin family 2:Holins are a diverse family of proteins that cause bacterial membrane lysis during late-protein synthesis. It is thought that the temporal precision of holin-mediated lysis may occur through the buildup of a holin oligomer which causes the lysis. IPR007633;Phage_holin_2. TMHMM2 reporting 12 TMH's Present. no signal peptide.; Conserved hypothetical protein; hypothetical protein 3395108 4608814 azo3085 Azoarcus sp. BH72 hypothetical protein YP_934588.1 3393825 R 62928 CDS YP_934589.1 119899376 4607785 complement(3395119..3395355) 1 NC_008702.1 Conserved hypothetical protein. Homology to ws1191 of W. succinogenes of 36% (trembl|Q7MRM8). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3395355 4607785 azo3086 Azoarcus sp. BH72 hypothetical protein YP_934589.1 3395119 R 62928 CDS YP_934590.1 119899377 4607786 complement(3395348..3395812) 1 NC_008702.1 Conserved hypothetical protein. Homology to CV2710 of C.violaceum of 41% (trembl:Q7NUI8). Pfam: Hemerythrin HHE cation binding domain Iteration of the HHE family found it to be related to Hemerythrin. It also demonstrated that what has been described as a single domain in fact consists of two cation binding domains. Members of this family occur all across nature and are involved in a variety of processes. For instance, in Nereis diversicolor MP2_NERDI binds Cadmium so as to protect the organism from toxicity. However Hemerythrin is classically described as Oxygen-binding through two attached Fe2+ ions. And the bacterial Q7WX96_ALCEU is a regulator of response to NO, which suggests yet another set-up for its metal ligands. In Staphylococcus aureus P72360 has been noted to be important when the organism switches to living in environments with low oxygen concentrations; perhaps this protein acts as an oxygen store or scavenger. No signal peptide. No TMHs.; hypothetical protein 3395812 4607786 azo3087 Azoarcus sp. BH72 hypothetical protein YP_934590.1 3395348 R 62928 CDS YP_934591.1 119899378 4607787 3396033..3396458 1 NC_008702.1 Nitric-oxide reductase subunit C (EC 1.7.99.7) (Nitric oxide reductase cytochrome c subunit) (NOR small subunit).COMPONENT OF THE ANAEROBIC RESPIRATORY CHAIN THAT TRANSFORMS NITRATE TO DINITROGEN (DENITRIFICATION).54% IPR000345; CytC_heme_BS. Pfam; PF00034; Cytochrom_C; 1. SignalP: present TMHelix:1; High confidence in function and specificity; putative nitric-oxide reductase subunit C 3396458 norC 4607787 norC Azoarcus sp. BH72 putative nitric-oxide reductase subunit C YP_934591.1 3396033 D 62928 CDS YP_934592.1 119899379 4609396 3396487..3397866 1 NC_008702.1 Nitric-oxide reductase subunit B (EC 1.7.99.7) (Nitric oxide reductase cytochrome b subunit). 69% similarity to the P.stutzeri NorB protein. COMPONENT OF THE ANAEROBIC RESPIRATORY CHAIN THAT TRANSFORMS NITRATE TO DINITROGEN (DENITRIFICATION). NorB IS THE CATALYTIC SUBUNIT OF THE ENZYME COMPLEX. SHOWS PROTON PUMP ACTIVITY ACROSS THE MEMBRANE IN DENITRIFYING BACTERIAL CELLS. THE MONONITROGEN REDUCTION IS PROBABLY COUPLED TO ELECTRON TRANSPORT PHOSPHORYLATION.Belongs to the heme-copper respiratory oxidase family. Swiss Prot: Q59647. IPR000883; COX1. PF00115; COX1; 1. TMHMM:predicted transmembrane helix present. SignalP: present ccoN: cytochrome c oxidase cbb3-type; High confidence in function and specificity; putative nitric-oxide reductase subunit B 3397866 norB 4609396 norB Azoarcus sp. BH72 putative nitric-oxide reductase subunit B YP_934592.1 3396487 D 62928 CDS YP_934593.1 119899380 4609395 3397938..3398141 1 NC_008702.1 Conserved hypothetical protein. Homology to RS05091 of R.solanacearum of 76% (trembl:Q8Y1C8). No domains present. No TMHs. No signal peptide.; hypothetical protein 3398141 4609395 azo3090 Azoarcus sp. BH72 hypothetical protein YP_934593.1 3397938 D 62928 CDS YP_934594.1 119899381 4607788 complement(3398151..3398831) 1 NC_008702.1 Potassium uptake protein. Part of the constitutive potassium transport systems trkG and trkH. May regulate the transport activity of trkG and trkH systems. Binds to NAD+ and NADH.Peripherally bound to the inner side of the inner membrane via the trkG and trkH proteins. 41% NAD_BS. IPR006037; TrkAC. IPR003148; TrkA_N. Pfam:PF02080; TrkA_C; 1. PF02254; TrkA_N; 1. Pfam:KTN: NAD-binding domain.; Specificity unclear; putative potassium uptake protein 3398831 trkA1 4607788 trkA1 Azoarcus sp. BH72 putative potassium uptake protein YP_934594.1 3398151 R 62928 CDS YP_934595.1 119899382 4609969 complement(3398833..3400161) 1 NC_008702.1 Membrane component of the potassium uptake system. 38% Cat_transpt. IPR004772; K_transptTrk. Pfam: PF02386; TrkH; 1. SignalP.present. TMHelix:9.; High confidence in function and specificity; putative potassium uptake protein, membrane component 3400161 trkH1 4609969 trkH1 Azoarcus sp. BH72 putative potassium uptake protein, membrane component YP_934595.1 3398833 R 62928 CDS YP_934596.1 119899383 4609971 complement(3400221..3400913) 1 NC_008702.1 Two component system transcriptional regulatory protein, 53% Identity to TrEMBL;Q7W532,Q8XUU6. Has SMART;SM00448,REC, cheY-homologous receiver domain;IPR001789;CheY regulates the clockwise rotation of E. coli flagellar motors. This domain contains a phosphoacceptor site that is phosphorylated by histidine kinase homologues. Has OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription] Has PF00486, Transcriptional regulatory protein, C terminal;IPR001867 Trans_reg_C; This domain is almost always found associated with the response regulator receiver domain (see INTERPRO:IPR001789). It may play a role in DNA binding; two component system transcriptional regulatory protein 3400913 4609971 azo3093 Azoarcus sp. BH72 two component system transcriptional regulatory protein YP_934596.1 3400221 R 62928 CDS YP_934597.1 119899384 4607789 complement(3400910..3402439) 1 NC_008702.1 Probable sensor for high-affinity potassium transport system,; High confidence in function and specificity; sensor for high-affinity potassium transport system 3402439 kdpD2 4607789 kdpD2 Azoarcus sp. BH72 sensor for high-affinity potassium transport system YP_934597.1 3400910 R 62928 CDS YP_934598.1 119899385 4609101 3402633..3403433 1 NC_008702.1 Nitric-oxide reductase ATP/GTP binding component,NorQ. 68% FGGY_kin. TMHMM:present SignalP: present.; High confidence in function and specificity; nitric-oxide reductase 3403433 norQ 4609101 norQ Azoarcus sp. BH72 nitric-oxide reductase YP_934598.1 3402633 D 62928 CDS YP_934599.1 119899386 4609399 3403553..3404569 1 NC_008702.1 Conserved hypothetical iron-sulfur 4Fe-4S ferredoxin transmembrane protein. Homology to pbprb0648 of P. profundum (tremblnew|CAG22521). Involved in a membrane generated redox signal. Pfam: 4Fe-4S binding domain no signal peptide 4 TMHs; Conserved hypothetical protein; iron-sulfur 4Fe-4S ferredoxin transmembrane protein 3404569 4609399 azo3096 Azoarcus sp. BH72 iron-sulfur 4Fe-4S ferredoxin transmembrane protein YP_934599.1 3403553 D 62928 CDS YP_934600.1 119899387 4607790 3404573..3406684 1 NC_008702.1 Nitric oxide reductase NorD protein. COMPONENT OF THE ANAEROBIC RESPIRATORY CHAIN THAT TRANSFORMS NITRATE TO DINITROGEN (DENITRIFICATION). FUNCTION UNKNOWN BUT ESSENTIAL FOR THE DENITRIFICATION PROCESS. 35% similarity to B. japonicum NorD. TREMBL:Q89QB3 InterPro:IPR002035; VWF_A. Pfam:PF00092; VWA; 1. InterPro:SM00327:von Willebrand factor type A domain SignalP:present. TMHMM: presence of TMHhelix.; High confidence in function and specificity; nitric-oxide reductase accessory cytoplasmic protein 3406684 norD 4607790 norD Azoarcus sp. BH72 nitric-oxide reductase accessory cytoplasmic protein YP_934600.1 3404573 D 62928 CDS YP_934601.1 119899388 4609397 3406687..3407451 1 NC_008702.1 Putative Fnr-like transcriptional activator,; Function unclear; putative Fnr-like transcriptional activator 3407451 4609397 azo3098 Azoarcus sp. BH72 putative Fnr-like transcriptional activator YP_934601.1 3406687 D 62928 CDS YP_934602.1 119899389 4607791 complement(3407478..3408710) 1 NC_008702.1 catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate; D-3-phosphoglycerate dehydrogenase 3408710 serA 4607791 serA Azoarcus sp. BH72 D-3-phosphoglycerate dehydrogenase YP_934602.1 3407478 R 62928 CDS YP_934603.1 119899390 4609865 complement(3408745..3410151) 1 NC_008702.1 Putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein. Homology to rdxB of R. sphaeroides of 39% (sprot|RDXB_RHOSH). Involved in a membrane generated redox signal; required to maintain repression of photosynthesis gene expression in the presence of oxygen. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450) Pfam: 4Fe-4S binding doamin no signal peptide probable 5 TMHs; Family membership; putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein 3410151 rdxB 4609865 rdxB Azoarcus sp. BH72 putative iron-sulfur 4Fe-4S ferredoxin transmembrane protein YP_934603.1 3408745 R 62928 CDS YP_934604.1 119899391 4609701 3410296..3410757 1 NC_008702.1 AAU3 protein precursor,; High confidence in function and specificity; AAU3 protein 3410757 aau3 4609701 aau3 Azoarcus sp. BH72 AAU3 protein YP_934604.1 3410296 D 62928 CDS YP_934605.1 119899392 4608245 complement(3410759..3411988) 1 NC_008702.1 Conserved hypothetical membrane protein,; Conserved hypothetical protein; hypothetical protein 3411988 4608245 azo3102 Azoarcus sp. BH72 hypothetical protein YP_934605.1 3410759 R 62928 CDS YP_934606.1 119899393 4607792 3412240..3412638 1 NC_008702.1 Conserved hypothetical cytochrome c5. Homology to nosC of A. eutrophus of 62% (trembl|Q7WX89). This basic c-type monoheme cytochrome has an unusually low redox potential compared with mitochondrial cytochrome c. It is reactive with cytochrome c oxidases but not with reductases. InterPro: Cytochrome c class I (IPR003088) Pfam: Cytorchrome C. signal peptide. no TMHs; Family membership; cytochrome c5 3412638 nosC 4607792 nosC Azoarcus sp. BH72 cytochrome c5 YP_934606.1 3412240 D 62928 CDS YP_934607.1 119899394 4609400 complement(3412697..3412969) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3412969 4609400 azo3104 Azoarcus sp. BH72 hypothetical protein YP_934607.1 3412697 R 62928 CDS YP_934608.1 119899395 4607793 complement(3413017..3413235) 1 NC_008702.1 Conserved hypothetical protein. Homology to Rgel02003456 of Rubrivivax gelatinosus of 68% (gi|47572283|ref|ZP_00242328.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 3413235 4607793 azo3105 Azoarcus sp. BH72 hypothetical protein YP_934608.1 3413017 R 62928 CDS YP_934609.1 119899396 4607794 complement(3413290..3413937) 1 NC_008702.1 conserved hypothetical secreted protein. Homology to TdenA01000391 of Thiobacillus denitrificans of 42% (gi|52008094|ref|ZP_00335471.1|(NBCI ENTREZ)). no domians predicted. signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 3413937 4607794 azo3106 Azoarcus sp. BH72 hypothetical protein YP_934609.1 3413290 R 62928 CDS YP_934610.1 119899397 4607795 complement(3413940..3414467) 1 NC_008702.1 Putative NosL protein. Homology to nosL of S. meliloti of 38% (trembl|O07331). NosL is one of the accessory proteins of the nos (nitrous oxide reductase) gene cluster. NosL is a monomeric protein of 18,540 MW that specifically and stoichiometrically binds Cu(I). The copper ion in NosL is ligated by a Cys residue, and one Met and one His are thought to serve as the other ligands. It is possible that NosL is a copper chaperone involved in metallocenter assembly. probable signal peptide no TMHs; Family membership; putative NosL protein 3414467 nosL 4607795 nosL Azoarcus sp. BH72 putative NosL protein YP_934610.1 3413940 R 62928 CDS YP_934611.1 119899398 4609403 complement(3414476..3415291) 1 NC_008702.1 Membrane protein nosY precursor.INVOLVED IN COPPER PROCESSING. Part of the ABC transporter complex nosDFY involved in copper import. Probably responsible for the translocation of the substrate across the membrane. 43% Similar to the copper permease, nosY in P.stutzeri. TREMBL:Q9F0W1 InterPro:IPR001117; Cu-oxidase. SignalP: present. Number of predicted TMHs:6; High confidence in function and specificity; putative copper transport system permease 3415291 nosY 4609403 nosY Azoarcus sp. BH72 putative copper transport system permease YP_934611.1 3414476 R 62928 CDS YP_934612.1 119899399 4609406 complement(3415299..3416243) 1 NC_008702.1 Copper transport ATP-binding protein nosF.INVOLVED IN COPPER PROCESSING/TRANSPORT.Part of the ABC transporter complex nosDFY involved in copper import. 3% ABC_tran; 1. InterPro:IPR003593; AAA_ATPase. IPR003439; ABC_transporter. InterPro:PS50101:ATP/GTP-binding site motif A (P-loop). InterPro:SM00382:AAA ATPase superfamily.; High confidence in function and specificity; putative copper transport system ATP-binding protein 3416243 nosF 4609406 nosF Azoarcus sp. BH72 putative copper transport system ATP-binding protein YP_934612.1 3415299 R 62928 CDS YP_934613.1 119899400 4609402 complement(3416272..3417549) 1 NC_008702.1 Copper-binding periplasmic protein precursor. INVOLVED IN COPPER PROCESSING AND TRANSPORT; IN THE ASSEMBLY OF THE COPPER CHROMOPHORES OF NITROUS OXIDE REDUCTASE.Part of the ABC transporter complex nosDFY involved in copper import. Similar to the putative periplasmic-binding protein nosD precursor from: P.fluorescens (36%)TREMBL:Q9F0W3 InterPro:IPR006633; CASH. IPR007742; NosD.IPR006626; PbH1. Pfam:PF05048; NosD; 1. Signal peptide: present.; High confidence in function and specificity; putative copper transport system periplasmic-binding protein 3417549 nosD 4609402 nosD Azoarcus sp. BH72 putative copper transport system periplasmic-binding protein YP_934613.1 3416272 R 62928 CDS YP_934614.1 119899401 4609401 complement(3417557..3417988) 1 NC_008702.1 Conserved hypothetical transcriptional activator for nitrous-oxide reductase, C-termial domain. Homology to nosr (C-terminal domain) of Azoarcus sp. EbN1 of 74% (gnl|keqq|eba:ebB224(KEGG)). no domains predicted. no signal peptide. probable 3 TMHs; Conserved hypothetical protein; hypothetical protein 3417988 nosRb 4609401 nosRb Azoarcus sp. BH72 hypothetical protein YP_934614.1 3417557 R 62928 CDS YP_934615.1 119899402 4609405 complement(3418003..3420174) 1 NC_008702.1 Regulatory protein nosR. Transcriptional activation of the nitrous-oxide reductase gene nosZ. Similar to SWISSPROT: sprot|NOSR_PSEST (37% Pseudomonas stutzeri (Pseudomonas perfectomarina), regulatory protein NosR). TMHMM reporting 5 transmembrane helices. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; transcriptional activator 3420174 nosRa 4609405 nosRa Azoarcus sp. BH72 transcriptional activator YP_934615.1 3418003 R 62928 CDS YP_934616.1 119899403 4609404 complement(3420337..3422271) 1 NC_008702.1 reduces nitrous oxide to nitrogen; nitrous-oxide reductase 3422271 nosZ 4609404 nosZ Azoarcus sp. BH72 nitrous-oxide reductase YP_934616.1 3420337 R 62928 CDS YP_934617.1 119899404 4609407 complement(3422454..3422729) 1 NC_008702.1 Hypothetical secreted protein. no homology to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 3422729 4609407 azo3114 Azoarcus sp. BH72 hypothetical protein YP_934617.1 3422454 R 62928 CDS YP_934618.1 119899405 4610019 complement(3423208..3424434) 1 NC_008702.1 catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP, in Bacillus, lysine sensitive; regulated by response to starvation.; aspartate kinase 3424434 lysC 4610019 lysC Azoarcus sp. BH72 aspartate kinase YP_934618.1 3423208 R 62928 CDS YP_934619.1 119899406 4609186 3424699..3424995 1 NC_008702.1 Conserved hypothetical protein. Homology to NMA0336 of N.meningitidis of 59% (trembl:Q9JRG4) Has PF01381 Helix-turn-helix:IPR001387:HTH_3;This is large family of DNA binding helix-turn helix proteins that include a bacterial plasmid copy control protein, bacterial methylases,various bacteriophage transcription control proteins and a vegetative specific protein from Dictyostelium discoideum. No signal peptide or TMH reported present.; hypothetical protein 3424995 4609186 azo3116 Azoarcus sp. BH72 hypothetical protein YP_934619.1 3424699 D 62928 CDS YP_934620.1 119899407 4607797 complement(3425017..3425976) 1 NC_008702.1 Conserved hypothetical protein. Homology to ne1518 of N. europaea of 43% (trembl|Q82UG8). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3425976 4607797 azo3117 Azoarcus sp. BH72 hypothetical protein YP_934620.1 3425017 R 62928 CDS YP_934621.1 119899408 4607798 3425991..3426446 1 NC_008702.1 Lyase, putative 6-pyruvoyl tetrahydrobiopterin synthase protein InterPro: 6-pyruvoyl tetrahydropterin synthase TIGRFAM: 6PTHBS: 6-pyruvoyl tetrahydrobiopterin synthase; Specificity unclear; 6-pyruvoyl-tetrahydropterin synthase 3426446 4607798 azo3118 Azoarcus sp. BH72 6-pyruvoyl-tetrahydropterin synthase YP_934621.1 3425991 D 62928 CDS YP_934622.1 119899409 4607799 complement(3426502..3427671) 1 NC_008702.1 Entry name:- TREMBL:Q8XWM4 InterPro:- IPR001522; Desaturase. IPR005804; Fa_desat. Pfam:- PF00487; FA_desaturase; 1. Identities = 244/390 (62%) Prediction: Non-secretory protein Signal peptide probability: 0.008 Number of predicted TMHs: 3; Family membership; fatty acid desaturase 3427671 4607799 azo3119 Azoarcus sp. BH72 fatty acid desaturase YP_934622.1 3426502 R 62928 CDS YP_934623.1 119899410 4607800 complement(3427710..3429119) 1 NC_008702.1 Region start changed from 3429549 to 3429093 (-456 bases); mechanosensitive ion channel 3429119 4607800 azo3120 Azoarcus sp. BH72 mechanosensitive ion channel YP_934623.1 3427710 R 62928 CDS YP_934624.1 119899411 4607801 complement(3429122..3429700) 1 NC_008702.1 Putative endonuclease,42% Identity to TrEMBL;Q8XWM2, 33% Identity to TrEMBL;Q8VMF9. Has Signal peptide. Has PLDc, Phospholipase D. Active site motifs; The PLD superfamily includes enzymes involved in signal transduction, lipid biosynthesis, endonucleases and open reading frames in pathogenic viruses and bacteria. PLD hydrolyzes the terminal phosphodiester bond of phospholipids to phosphatidic acid and a hydrophilic constituent. Phosphatidic acid is a compound that is heavily involved in signal transduction. The common features of the family members are that they can bind to a phosphodiester moiety, and that most of these enzymes are active as bi-lobed monomers or dimers.; putative endonuclease 3429700 4607801 azo3121 Azoarcus sp. BH72 putative endonuclease YP_934624.1 3429122 R 62928 CDS YP_934625.1 119899412 4607802 complement(3429690..3430982) 1 NC_008702.1 SUN protein; Specificity unclear; hypothetical protein 3430982 4607802 azo3122 Azoarcus sp. BH72 hypothetical protein YP_934625.1 3429690 R 62928 CDS YP_934626.1 119899413 4607803 complement(3430991..3432181) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA651 of Azoarcus sp. EbN1 of 37% (gnl|keqq|eba:ebA651(KEGG)). InterproterPro (IPR000694): Proline-rich region. SignalP reporting signal peptide. no TMHs; hypothetical protein 3432181 4607803 azo3123 Azoarcus sp. BH72 hypothetical protein YP_934626.1 3430991 R 62928 CDS YP_934627.1 119899414 4607804 complement(3432188..3432829) 1 NC_008702.1 glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate; phosphoribosylglycinamide formyltransferase 3432829 purN 4607804 purN Azoarcus sp. BH72 phosphoribosylglycinamide formyltransferase YP_934627.1 3432188 R 62928 CDS YP_934628.1 119899415 4607805 complement(3432869..3433585) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to orf58 of Pseudomonas sp. of 42% (trembl|Q937A2(SRS)). No domains predicted. Signal P predicts signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3433585 4607805 azo3125 Azoarcus sp. BH72 hypothetical protein YP_934628.1 3432869 R 62928 CDS YP_934629.1 119899416 4607806 3433752..3435617 1 NC_008702.1 DNA mismatch repair protein mutL. This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a molecular matchmaker a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex (By similarity). InterPro: DNA mismatch repair proteins mutL/hexB/PMS1 mutl: DNA mismatch repair protein MutL; High confidence in function and specificity; DNA mismatch repair protein 3435617 mutL 4607806 mutL Azoarcus sp. BH72 DNA mismatch repair protein YP_934629.1 3433752 D 62928 CDS YP_934630.1 119899417 4609296 complement(3435648..3436235) 1 NC_008702.1 Hypothetical secreted protein no homology of the entire protein to a protein of similar lengt in the data bank no domains predicted signal peptide no TMHs; hypothetical protein 3436235 4609296 azo3127 Azoarcus sp. BH72 hypothetical protein YP_934630.1 3435648 R 62928 CDS YP_934631.1 119899418 4607807 3436442..3436924 1 NC_008702.1 Inhibits transcription at high concentrations of nickel; nickel responsive regulator 3436924 nikR 4607807 nikR Azoarcus sp. BH72 nickel responsive regulator YP_934631.1 3436442 D 62928 CDS YP_934632.1 119899419 4609379 3437106..3437372 1 NC_008702.1 Conserved hypothetical protein. Homology to orfJ of E. coli of 33% (trembl|O70046). no domains predicted .no signal peptide. no TMHs.; hypothetical protein 3437372 4609379 azo3129 Azoarcus sp. BH72 hypothetical protein YP_934632.1 3437106 D 62928 CDS YP_934633.1 119899420 4607808 complement(3437383..3438279) 1 NC_008702.1 catalyzes the formation of a phosphodiester at the site of a single-strand break in duplex DNA; DNA ligase 3438279 dnaL 4607808 dnaL Azoarcus sp. BH72 DNA ligase YP_934633.1 3437383 R 62928 CDS YP_934634.1 119899421 4608637 3438489..3439451 1 NC_008702.1 IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity; tRNA delta(2)-isopentenylpyrophosphate transferase 3439451 miaA 4608637 miaA Azoarcus sp. BH72 tRNA delta(2)-isopentenylpyrophosphate transferase YP_934634.1 3438489 D 62928 CDS YP_934635.1 119899422 4609223 3439484..3440029 1 NC_008702.1 Hypothetical protein, 56% identity(71% similarity) with TrEMBL;Q6LK82 Has PF07209, Protein of unknown function (DUF1415);IPR009858; This family consists of several hypothetical bacterial proteins of around 180 residues in length. The function of this family is unknown. Signal peptide or TMH not present. EIIA-man: PTS system fructose subfamily; hypothetical protein 3440029 4609223 azo3132 Azoarcus sp. BH72 hypothetical protein YP_934635.1 3439484 D 62928 CDS YP_934636.1 119899423 4607809 complement(3440085..3440531) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology of the protein to a protein of similar size. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3440531 4607809 azo3133 Azoarcus sp. BH72 hypothetical protein YP_934636.1 3440085 R 62928 CDS YP_934637.1 119899424 4607810 complement(3440553..3443636) 1 NC_008702.1 AcrB/AcrD/AcrF family. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q9HW27 (38% identity); SWISSPROT:Q57124 (33% identity). InterPro (IPR001036): Acriflavin resistance protein. Pfam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TMHMM predicting 12 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 3443636 4607810 azo3134 Azoarcus sp. BH72 RND efflux transporter permease YP_934637.1 3440553 R 62928 CDS YP_934638.1 119899425 4607811 complement(3443646..3444743) 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family, TREMBL:Q7W437 (52% identity); TREMBL:Q8EFT5 (32% identity). InterPro (IPR006143): Secretion protein HlyD. Pfam (PF00529): HlyD family secretion protein. TMHMM reporting one transmembrane helix. TC (8.A.1): The Membrane Fusion Protein (MFP) Family.; Family membership; membrane fusion protein 3444743 4607811 azo3135 Azoarcus sp. BH72 membrane fusion protein YP_934638.1 3443646 R 62928 CDS YP_934639.1 119899426 4607812 complement(3444879..3445937) 1 NC_008702.1 catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis; phosphoribosylaminoimidazole synthetase 3445937 purM 4607812 purM Azoarcus sp. BH72 phosphoribosylaminoimidazole synthetase YP_934639.1 3444879 R 62928 CDS YP_934640.1 119899427 4609663 3446157..3447275 1 NC_008702.1 Conserved hypothetical membrane protein. This is a family of hypothetical proteins. A number of the sequence records state they are transmembrane proteins or putative permeases.It is not clear what source suggested that these proteins might be permeases and this information should be treated with caution. TREMBL:Q82Y01: 39% identity; 58% similarity. TREMBL:Q8XW51: 37% identity; 53% similarity. InterPro:IPR002549 Pfam: PF01594:Protein of unknown function DUF70 Signal P predicted signal peptide and TMHMM predicted transmembrane helices; Conserved hypothetical protein; hypothetical protein 3447275 4609663 azo3137 Azoarcus sp. BH72 hypothetical protein YP_934640.1 3446157 D 62928 CDS YP_934641.1 119899428 4607813 3447272..3447961 1 NC_008702.1 Region start changed from 3447165 to 3447246 (-81 bases); DnaA regulatory inactivator Hda 3447961 4607813 azo3138 Azoarcus sp. BH72 DnaA regulatory inactivator Hda YP_934641.1 3447272 D 62928 CDS YP_934642.1 119899429 4607814 3447999..3448661 1 NC_008702.1 Haloacid dehalogenase-like hydrolase family is structurally different from the alpha/beta hydrolase family (abhydrolase). This family includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. Similar to trembl|Q82XZ3 (63%) and to pir|B83604 (47%). Pfam (PF00702): haloacid dehalogenase-like hydrolase; Family membership; haloacid dehalogenase-like hydrolase family protein 3448661 4607814 azo3139 Azoarcus sp. BH72 haloacid dehalogenase-like hydrolase family protein YP_934642.1 3447999 D 62928 CDS YP_934643.1 119899430 4607815 3448752..3450110 1 NC_008702.1 Probable Poly(A) polymerase. Homology to pcnB of E. coli of 41% (sprot|PCNB_ECOLI) POLYMERASE THAT CREATES THE 3POLY(A) TAIL FOUND IN SOME MRNAS. SEEMS TO BE INVOLVED IN PLASMID COPY NUMBER CONTROL. Pfam: Poly A polymerase family no signal peptide no TMHs; Family membership; poly(A) polymerase 3450110 pcnB 4607815 pcnB Azoarcus sp. BH72 poly(A) polymerase YP_934643.1 3448752 D 62928 CDS YP_934644.1 119899431 4609512 3450110..3450607 1 NC_008702.1 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK). InterPro: 7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase Pfam:7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; High confidence in function and specificity; 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase 3450607 folK 4609512 folK Azoarcus sp. BH72 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase YP_934644.1 3450110 D 62928 CDS YP_934645.1 119899432 4608822 3450614..3450997 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA1577 of Pseudomonas aeruginosa of 69% (trembl|Q9I3E0). Has PF04342, IPR007437;Protein of unknown function, DUF486;This family contains several proteins of uncharacterised function. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 3450997 4608822 azo3142 Azoarcus sp. BH72 hypothetical protein YP_934645.1 3450614 D 62928 CDS YP_934646.1 119899433 4607816 3451005..3451646 1 NC_008702.1 Deoxyguanosine kinase, probable (2.7.1.113) InterPro: Thymidylate kinase DTMP_kinase: thymidylate kinase; Family membership; putative deoxyguanosine kinase 3451646 4607816 azo3143 Azoarcus sp. BH72 putative deoxyguanosine kinase YP_934646.1 3451005 D 62928 CDS YP_934647.1 119899434 4607817 3451731..3452546 1 NC_008702.1 catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate; 3-methyl-2-oxobutanoate hydroxymethyltransferase 3452546 panB 4607817 panB Azoarcus sp. BH72 3-methyl-2-oxobutanoate hydroxymethyltransferase YP_934647.1 3451731 D 62928 CDS YP_934648.1 119899435 4609493 3452563..3453390 1 NC_008702.1 catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine; pantoate--beta-alanine ligase 3453390 panC 4609493 panC Azoarcus sp. BH72 pantoate--beta-alanine ligase YP_934648.1 3452563 D 62928 CDS YP_934649.1 119899436 4609494 3453480..3453968 1 NC_008702.1 Region start changed from 3453406 to 3453454 (-48 bases); hypothetical protein 3453968 4609494 azo3146 Azoarcus sp. BH72 hypothetical protein YP_934649.1 3453480 D 62928 CDS YP_934650.1 119899437 4607818 complement(3454036..3456720) 1 NC_008702.1 Aminopeptidase catalyzes the removal of single amino acids from the amino terminus of small peptides and probable plays a role in their final digestion. Similar to trembl|Q8XXI8 (50%) and to sprot|AMPN_ECOLI (47%). Pfam (PF01433): Aminopeptidase N, APN (CD13); Specificity unclear; aminopeptidase N 3456720 pepN 4607818 pepN Azoarcus sp. BH72 aminopeptidase N YP_934650.1 3454036 R 62928 CDS YP_934651.1 119899438 4609522 complement(3456803..3457705) 1 NC_008702.1 universal stress protein 3457705 uspA 4609522 uspA Azoarcus sp. BH72 universal stress protein YP_934651.1 3456803 R 62928 CDS YP_934652.1 119899439 4610079 complement(3457702..3460452) 1 NC_008702.1 Probable cation-transporting ATPase (EC 3.6.3.-).Could mediate calcium influx.Integral membrane protein.Belongs to the cation transport ATPases family (P-type ATPases). Subfamily IIA. 47% similarity to the cyanobacterium Synechocystis sp. PCC 6803, pma1. protein. SWISSPROT:ATA1_SYNY3.P37367 InterPro:IPR001757; ATPase_E1-E2.IPR006068; Cation_ATPase_C.IPR004014; Cation_ATPase_N.IPR008250; E1-E2_ATPase_reg.IPR000695; H_ATPase.IPR005834; Hydrolase. Pfam:PF00689: Cation_ATPase_C;1. PF00690: Cation_ATPase_N;1. PF00122: E1-E2_ATPase;1. PF00702: Hydrolase; 1. SignalP: present. InterPro:PF00702:Haloacid dehalogenase/epoxide hydrolase family.; High confidence in function and specificity; putative cation-transporting P-type ATPase 3460452 pma1 4610079 pma1 Azoarcus sp. BH72 putative cation-transporting P-type ATPase YP_934652.1 3457702 R 62928 CDS YP_934653.1 119899440 4609586 complement(3460566..3461057) 1 NC_008702.1 Conserved hypothetical RDD family protein. Homology to ne0432 of N. europaea of 42% (trembl|Q82X63(SRS)). Pfam: (by Smart) RDD family This family of proteins contain three highly conserved amino acids: one arginine and two aspartates, hence the name of RDD family. This region contains two predicted transmembrane regions. The arginine occurs at the N terminus of the first helix and the first aspartate occurs in the middle of this helix. The molecular function of this region is unknown. However this region may be involved in transport of an as yet unknown set of ligands. signal peptide. 3 TMHs; Family membership; putative RDD family protein 3461057 4609586 azo3150 Azoarcus sp. BH72 putative RDD family protein YP_934653.1 3460566 R 62928 CDS YP_934654.1 119899441 4607819 complement(3461066..3461431) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA7126 of Azoarcus sp. EbN1 of 45% (gnl|keqq|eba:ebA7126(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3461431 4607819 azo3151 Azoarcus sp. BH72 hypothetical protein YP_934654.1 3461066 R 62928 CDS YP_934655.1 119899442 4607820 complement(3461470..3461880) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03002551 of Dechloromonas aromatica of 31% (gi|41723955|ref|ZP_00150845.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3461880 4607820 azo3152 Azoarcus sp. BH72 hypothetical protein YP_934655.1 3461470 R 62928 CDS YP_934656.1 119899443 4607821 complement(3461877..3462467) 1 NC_008702.1 Probable RNA polymerase sigma factor, 62% Identity to TrEMBL;Q8XXN5, Q7P0I2. Has PF04542, Sigma-70 region 2;IPR007627, Sigma70_r2; Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA polymerase binding determinant. The core binding helix,interacts with the clamp domain of the largest polymerase subunit, beta prime. The aromatic residues of the recognition helix, found at the C-terminus of this domain are though to mediate strand separation, thereby allowing transcription initiation. Has PF04545, Sigma-70, region 4;IPR007630, Sigma70_r4; Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix families. Therefore there are many false negatives. PF00196,Bacterial regulatory proteins, luxR family; The many bacterial transcription regulation proteins which bind DNA through a 'helix-turn-helix' motif can be classified into subfamilies on the basis of sequence similarities. One of these subfamilies which includes proteins with sizes ranging from 74 (gerE) to 901 amino acids (malT), can be further subdivided into two classes on the basis of the mechanism by which they are activated. The first is a class of regulators which belong to a two-component sensory transduction system where the protein is activated by its phosphorylation, generally on an aspartate residue,by a transmembrane kinase. The proteins that belong to this class include bvgA, comA, dctR; degU, evgA, fimZ,fixJ, gacA, glpR, narL, narP, nodW, rcsB and uhpA. The second is a class of regulators which is activated when bound to autoinducer molecules such as N-(3-oxohexanoyl)-L-homoserine lactone (OHHL. The proteins that belong to this class are carR, echR, esaR, expR, lasR, luxR, phzR, rhlR,traR and yenR. The 'helix-turn-helix' DNA-binding motif of these proteins is located in the C-terminal section of the sequence.; RNA polymerase sigma factor 3462467 4607821 azo3153 Azoarcus sp. BH72 RNA polymerase sigma factor YP_934656.1 3461877 R 62928 CDS YP_934657.1 119899444 4607822 3462564..3464276 1 NC_008702.1 Acetolactate synthase isozyme III large subunit (AHAS- III) (Acetohydroxy-acid synthase III large subunit) (ALS-III). InterPro: Acetolactate synthase large subunit biosynthetic type TIGRFAM: acolac_lg: acetolactate synthase large subunit, biosynthetic type; High confidence in function and specificity; acetolactate synthase 3464276 ilvI 4607822 ilvI Azoarcus sp. BH72 acetolactate synthase YP_934657.1 3462564 D 62928 CDS YP_934658.1 119899445 4609075 3464331..3464822 1 NC_008702.1 with IlvI catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase 3 small subunit; acetolactate synthase 3 regulatory subunit 3464822 ilvH 4609075 ilvH Azoarcus sp. BH72 acetolactate synthase 3 regulatory subunit YP_934658.1 3464331 D 62928 CDS YP_934659.1 119899446 4609074 3464912..3465928 1 NC_008702.1 catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis; ketol-acid reductoisomerase 3465928 ilvC 4609074 ilvC Azoarcus sp. BH72 ketol-acid reductoisomerase YP_934659.1 3464912 D 62928 CDS YP_934660.1 119899447 4609070 3466062..3466856 1 NC_008702.1 This enzyme, CDP-diacylglycerol--serine O-phosphatidyltransferase, is involved in phospholipid biosynthesis catalyzing the reaction CDP-diacylglycerol + L-serine = CMP + L-1-phosphatidylserine. Entry name:-SWISSPROT:PSS_HELPY identity:- 34% InterPro:- IPR000462; CDP-OH_P_trans. IPR004533; PssA. Pfam:- PF01066; CDP-OH_P_transf; 1. Number of predicted TMHs: 6 Prediction: Non-secretory protein Signal peptide probability: 0.047(YES); Family membership; putative CDP-diacylglycerol--serine O-phosphatidyltransferase 3466856 pssA 4609070 pssA Azoarcus sp. BH72 putative CDP-diacylglycerol--serine O-phosphatidyltransferase YP_934660.1 3466062 D 62928 CDS YP_934661.1 119899448 4609640 3466884..3467567 1 NC_008702.1 Two-component system regulatory protein ColR,; High confidence in function and specificity; two-component system regulatory protein 3467567 colR 4609640 colR Azoarcus sp. BH72 two-component system regulatory protein YP_934661.1 3466884 D 62928 CDS YP_934662.1 119899449 4608508 3467557..3468843 1 NC_008702.1 Two-component system sensor protein ColS,; High confidence in function and specificity; two-component system sensor protein 3468843 colS 4608508 colS Azoarcus sp. BH72 two-component system sensor protein YP_934662.1 3467557 D 62928 CDS YP_934663.1 119899450 4608509 3468913..3469608 1 NC_008702.1 Similar to TREMBL:P95473 (66% identity); TREMBL:Q87X18 (66% identity); TREMBL:Q87TV0 (61% identity).; hypothetical protein 3469608 4608509 azo3160 Azoarcus sp. BH72 hypothetical protein YP_934663.1 3468913 D 62928 CDS YP_934664.1 119899451 4607823 3469605..3470279 1 NC_008702.1 Conserved hypothetical InaA protein. Homology to inaA of P. putida of 39% (gnl|keqq|ppu:PP0904(KEGG)). Pfam: Lipopolysaccharide kinase (Kdo/WaaP) family. These lipopolysaccharide kinases are related to protein kinases Pkinase. This family includes waaP (rfaP) gene product is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide (LPS) inner core region. It has previously been shown that WaaP is necessary for resistance to hydrophobic and polycationic antimicrobials in E. coli and that it is required for virulence in invasive strains of S. enterica. No signal peptide. No TMHs; Conserved hypothetical protein; InaA protein 3470279 inaA 4607823 inaA Azoarcus sp. BH72 InaA protein YP_934664.1 3469605 D 62928 CDS YP_934665.1 119899452 4609080 3470652..3472187 1 NC_008702.1 catalyzes the formation of 2-isopropylmalate from acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis; 2-isopropylmalate synthase 3472187 leuA1 4609080 leuA1 Azoarcus sp. BH72 2-isopropylmalate synthase YP_934665.1 3470652 D 62928 CDS YP_934666.1 119899453 4609129 complement(3472263..3472613) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to bb2106 of B. bronchispetica of 55% (trembl|Q7WKK0(SRS)). No domains predicted. signal peptide. 2 TMHs.; Conserved hypothetical protein; hypothetical protein 3472613 4609129 azo3163 Azoarcus sp. BH72 hypothetical protein YP_934666.1 3472263 R 62928 CDS YP_934667.1 119899454 4607824 complement(3472610..3473209) 1 NC_008702.1 Flavoprotein wrbA. TREMBL:Q8XZ48:63% identity, 73% similarity; Q7NZW0: 61%, 72% WrbA, related to and slightly larger than flavodoxin. In Escherichia coli, this protein is produced during stationary phase, binds to the trp repressor, and makes trp operon repression more efficient. WrbA does not interact with the trp operator by itself. Three copies are found in Sinorhizobium meliloti InterPro: IPR008254; Flav_nitox_synth. IPR001226; Flavodoxin. Pfam PF00258: flavodoxin Absence of signal peptide (Signal P predicted) and Transmembrane helices (TMHMM predicted). wrbA2 identical to wrbA1 and wrbA3 by 37% and 23% respectively.; High confidence in function and specificity; tryptophan repressor binding protein 3473209 wrbA2 4607824 wrbA2 Azoarcus sp. BH72 tryptophan repressor binding protein YP_934667.1 3472610 R 62928 CDS YP_934668.1 119899455 4610113 3473290..3474516 1 NC_008702.1 Putative membrane protein(Ribonuclease BN)36% identity to TrEMBL; Q7NZV9.TrEMBL;Q8XZ47(27% identity). Has PF03631, Ribonuclease BN-like family;IPR004664,RNase_BN;This family contains integral membrane proteins with 5 to 6 predicted transmembrane spans. The family include ribonuclease BN that is involved in tRNA maturation P32146. This family of proteins does not seem to contain any completely conserved polar residues that would be expected in a nuclease enzyme, suggesting that many members of this family may not have this catalytic activity.; High confidence in function and specificity; exoribonuclease II 3474516 4610113 azo3165 Azoarcus sp. BH72 exoribonuclease II YP_934668.1 3473290 D 62928 CDS YP_934669.1 119899456 4607825 complement(3474504..3476027) 1 NC_008702.1 Hypothetical Protein,20% identity to TrEMBL;Q9HUK7. Very Bad homology with the hits in the DB over entire length of protein. No substantial good hits available. No signal peptide or TMH reported present.; hypothetical protein 3476027 4607825 azo3166 Azoarcus sp. BH72 hypothetical protein YP_934669.1 3474504 R 62928 CDS YP_934670.1 119899457 4607826 3476239..3476550 1 NC_008702.1 50S ribosomal protein L21, 83% Identity to TrEMBL;Q7WQL6,Q7W1P0, Q8XVK8. Has PF00829, Ribosomal prokaryotic L21 protein;IPR001787 Ribosomal_L21p; Ribosomes are the particles that catalyze mRNA-directed protein synthesis in all organisms. The codons of the mRNA are exposed on the ribosome to allow tRNA binding. This leads to the incorporation of amino acids into the growing polypeptide chain in accordance with the genetic information. Incoming amino acid monomers enter the ribosomal A site in the form of aminoacyl-tRNAs complexed with elongation factor Tu (EF-Tu) and GTP. The growing polypeptide chain, situated in the P site as peptidyl-tRNA, is then transferred to aminoacyl-tRNA and the new peptidyl-tRNA, extended by one residue, is translocated to the P site with the aid the elongation factor G (EF-G) and GTP as the deacylated tRNA is released from the ribosome through one or more exit sites MEDLINE:11297922,MEDLINE:11290319. About 2/3 of the mass of the ribosome consists of RNA and 1/3 of protein. The proteins are named in accordance with the subunit of the ribosome which they belong to - the small (S1 to S31) and the large (L1 to L44). Usually they decorate the rRNA cores of the subunits.; 50S ribosomal protein L21 3476550 rplU 4607826 rplU Azoarcus sp. BH72 50S ribosomal protein L21 YP_934670.1 3476239 D 62928 CDS YP_934671.1 119899458 4609780 3476566..3476841 1 NC_008702.1 involved in the peptidyltransferase reaction during translation; 50S ribosomal protein L27 3476841 rpmA 4609780 rpmA Azoarcus sp. BH72 50S ribosomal protein L27 YP_934671.1 3476566 D 62928 CDS YP_934672.1 119899459 4609785 3476915..3478141 1 NC_008702.1 essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication; GTPase ObgE 3478141 obgE 4609785 obgE Azoarcus sp. BH72 GTPase ObgE YP_934672.1 3476915 D 62928 CDS YP_934673.1 119899460 4607827 3478164..3479279 1 NC_008702.1 catalyzes the formation of glutamate 5-phosphate from glutamate in proline biosynthesis; gamma-glutamyl kinase 3479279 proB 4607827 proB Azoarcus sp. BH72 gamma-glutamyl kinase YP_934673.1 3478164 D 62928 CDS YP_934674.1 119899461 4609633 3479458..3480543 1 NC_008702.1 Conserved hypothetical protein. Homology to ne2236 of N. europaea of 58% (trembl:Q82SS1). No domains predicted. No signal peptide. No TMHs; hypothetical protein 3480543 4609633 azo3171 Azoarcus sp. BH72 hypothetical protein YP_934674.1 3479458 D 62928 CDS YP_934675.1 119899462 4607828 3480540..3482300 1 NC_008702.1 It belongs to the ATP-dependent AMP-binding enzyme family. Entry name Q9X7Y5 Primary accession number Q9X7Y5 Identities = 193/609 (31%) InterPro IPR000873; AMP-bind. Pfam PF00501; AMP-binding; 2 Signal peptide probability: 0.006 Number of predicted TMHs: 0; Family membership; putative long chain fatty acid CoA ligase 3482300 4607828 azo3172 Azoarcus sp. BH72 putative long chain fatty acid CoA ligase YP_934675.1 3480540 D 62928 CDS YP_934676.1 119899463 4607829 3482297..3483427 1 NC_008702.1 UDP-glucose 4-epimerase (EC 5.1.3.2) (Galactowaldenase) (UDP- galactose 4-epimerase). PLAYS AN ESSENTIAL ROLE IN THE INCORPORATION OF GALACTOSE INTO MENINGOCOCCAL LIPOPOLYSACCHARIDE SURFACE MOLECULES WHICH ARE IMPORTANT FOR PATHOGENESIS. hemL: glutamate-1-semialdehyde-21-amin; High confidence in function and specificity; UDP-glucose 4-epimerase 3483427 galE1 4607829 galE1 Azoarcus sp. BH72 UDP-glucose 4-epimerase YP_934676.1 3482297 D 62928 CDS YP_934677.1 119899464 4608855 complement(3483441..3484316) 1 NC_008702.1 InterPro (IPR000051): SAM (and some other nucleotide) binding motif. InterPro (IPR001601): Generic methyltransferase. Pfam (PF01209): ubiE/COQ5 methyltransferase family.; Function unclear; putative methyltransferase 3484316 4608855 azo3174 Azoarcus sp. BH72 putative methyltransferase YP_934677.1 3483441 R 62928 CDS YP_934678.1 119899465 4607830 complement(3484397..3485239) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. Signal Peptide present. No TMH present.; hypothetical protein 3485239 4607830 azo3175 Azoarcus sp. BH72 hypothetical protein YP_934678.1 3484397 R 62928 CDS YP_934679.1 119899466 4607831 3485581..3486774 1 NC_008702.1 Region start changed from 3485680 to 3485581 (99 bases); ABC transporter permease 3486774 4607831 azo3176 Azoarcus sp. BH72 ABC transporter permease YP_934679.1 3485581 D 62928 CDS YP_934680.1 119899467 4607832 3486774..3487988 1 NC_008702.1 part of a lipoprotein translocation system (translocates lipoproteins from the inner membrane to periplasmic chaperone, LolA, which transfers the lipoproteins to an outer membrane receptor, LolB, which anchors the lipoprotein to the outer membrane of the Gram-negative bacterial cell envelope); Specificity unclear; ABC transporter permease 3487988 lolE3 4607832 lolE3 Azoarcus sp. BH72 ABC transporter permease YP_934680.1 3486774 D 62928 CDS YP_934681.1 119899468 4609172 3488167..3488946 1 NC_008702.1 Similar to TREMBL:Q82SR1 (47% identity).; hypothetical protein 3488946 4609172 azo3178 Azoarcus sp. BH72 hypothetical protein YP_934681.1 3488167 D 62928 CDS YP_934682.1 119899469 4607833 complement(3489012..3490904) 1 NC_008702.1 catalyzes the formation of propionyl-CoA using propionate as a substrate; PrpE from Ralstonia solanacearum can produce acetyl-, propionyl-, butyryl- and acrylyl-coenzyme A, and Salmonella enterica produces propionyl- and butyryl-coenzyme A; not expressed in Escherichia coli when grown on propionate/minimal media; ATP-dependent; propionyl-CoA synthetase 3490904 prpE 4607833 prpE Azoarcus sp. BH72 propionyl-CoA synthetase YP_934682.1 3489012 R 62928 CDS YP_934683.1 119899470 4609636 complement(3491000..3492196) 1 NC_008702.1 Putative response regulator protein,; Family membership; putative response regulator protein 3492196 4609636 azo3180 Azoarcus sp. BH72 putative response regulator protein YP_934683.1 3491000 R 62928 CDS YP_934684.1 119899471 4607834 complement(3492225..3493199) 1 NC_008702.1 Region start changed from 3493365 to 3493173 (-192 bases); dehydrogenase 3493199 4607834 azo3181 Azoarcus sp. BH72 dehydrogenase YP_934684.1 3492225 R 62928 CDS YP_934685.1 119899472 4607835 3493390..3494259 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003801 of Dechloromonas aromatica of 44% (gi|41722610|ref|ZP_00149605.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 3494259 4607835 azo3182 Azoarcus sp. BH72 hypothetical protein YP_934685.1 3493390 D 62928 CDS YP_934686.1 119899473 4607836 3494332..3494907 1 NC_008702.1 Putative cytoplasmic membrane protein, lemA, 41% identity(63% similarity) to TrEMBL;Q9FA50.TrEMBL;Q7CNK7(37% identity). Has PF04011,LemA family;IPR007156;The members of this family are related to the LemA protein P71452. LemA contains an amino terminal predicted transmembrane helix. It has been predicted that the small amino terminus is extracellular. The exact molecular function of this protein is uncertain.; Conserved hypothetical protein; hypothetical protein 3494907 lemA2 4607836 lemA2 Azoarcus sp. BH72 hypothetical protein YP_934686.1 3494332 D 62928 CDS YP_934687.1 119899474 4609126 3494927..3495829 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE0281 of Nitrosomonas europaea of 34% (trembl|Q82XI9(SRS)). No domains predicted. no signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 3495829 4609126 azo3184 Azoarcus sp. BH72 hypothetical protein YP_934687.1 3494927 D 62928 CDS YP_934688.1 119899475 4607837 complement(3495834..3497804) 1 NC_008702.1 ABC transporter ATP-binding protein uup-1. InterPro: AAA ATPase superfamily Presence of SMC domain at N-terminal. Occurence of HypB/UreG nucleotide binding domain. FERM and yrdC domains are also present. Presence of uncharacterized P-loop hydralase domain. Presence of signal peptide and transmembrane helices. 61% identity and 76% similarity to Chromobacterium ABC transporter.; Family membership; ABC transporter ATP-binding protein 3497804 abcZ 4607837 abcZ Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934688.1 3495834 R 62928 CDS YP_934689.1 119899476 4608246 3497849..3499915 1 NC_008702.1 Probable site-specific recombinase.; Function unclear; ORF7; hypothetical protein 3499915 4608246 azo3186 Azoarcus sp. BH72 hypothetical protein YP_934689.1 3497849 D 62928 CDS YP_934690.1 119899477 4609984 complement(3500067..3500861) 1 NC_008702.1 Conserved hypothetical serine protease. Homology to mucD of N. europaea of 50% (trembl|Q82XA8). InterPro: Serine proteases trypsin family (IPR001254); Chymotrypsin serin protease family (S1) (IPR001314). Pfam: Trypsin. signal peptide. no TMHs; Family membership; putative serine protease 3500861 4609984 azo3187 Azoarcus sp. BH72 putative serine protease YP_934690.1 3500067 R 62928 CDS YP_934691.1 119899478 4607839 complement(3500858..3501787) 1 NC_008702.1 Soluble lytic murein transglycosylase precursor (EC 3.2.1.-) (Slt70). Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division (By similarity). InterPro: SLT domain; Family membership; soluble lytic murein transglycosylase 3501787 4607839 azo3188 Azoarcus sp. BH72 soluble lytic murein transglycosylase YP_934691.1 3500858 R 62928 CDS YP_934692.1 119899479 4607840 complement(3501801..3503081) 1 NC_008702.1 CapL protein. Required for the biosynthesis of type 1 capsular polysaccharide. InterPro: UDP-glucose/GDP-mannose dehydrogenase family gutA: pts system glucitol/sorbitol-s; Specificity unclear; NDPsugar dehydrogenase 3503081 4607840 azo3189 Azoarcus sp. BH72 NDPsugar dehydrogenase YP_934692.1 3501801 R 62928 CDS YP_934693.1 119899480 4607841 complement(3503118..3505919) 1 NC_008702.1 In part; Function unclear; hypothetical protein 3505919 4607841 azo3190 Azoarcus sp. BH72 hypothetical protein YP_934693.1 3503118 R 62928 CDS YP_934694.1 119899481 4607842 complement(3505919..3507280) 1 NC_008702.1 Probable acetoacetate metabolism regulatory protein,; Specificity unclear; acetoacetate metabolism regulatory protein 3507280 atoC 4607842 atoC Azoarcus sp. BH72 acetoacetate metabolism regulatory protein YP_934694.1 3505919 R 62928 CDS YP_934695.1 119899482 4608365 complement(3507270..3509348) 1 NC_008702.1 Putative two component sensor histidine kinase,; Family membership; putative two component sensor histidine kinase 3509348 4608365 azo3192 Azoarcus sp. BH72 putative two component sensor histidine kinase YP_934695.1 3507270 R 62928 CDS YP_934696.1 119899483 4607843 complement(3509439..3510821) 1 NC_008702.1 Might transfer a sugar moiety directly to a lipd acceptor. Putative colanic biosynthesis UDP-glucose lipid carrier transferase. InterPro: Bacterial sugar transferase; Family membership; glycosyltransferase 3510821 4607843 azo3193 Azoarcus sp. BH72 glycosyltransferase YP_934696.1 3509439 R 62928 CDS YP_934697.1 119899484 4607844 3511072..3511518 1 NC_008702.1 hypothetical protein 3511518 4607844 azo3194 Azoarcus sp. BH72 hypothetical protein YP_934697.1 3511072 D 62928 CDS YP_934698.1 119899485 4608232 complement(3517200..3518531) 1 NC_008702.1 Hypothetical protein. No known Domains/Features/Signal Peptide or TMH present. Weak Homology with hits in the database.; hypothetical protein 3518531 4608232 azo3195 Azoarcus sp. BH72 hypothetical protein YP_934698.1 3517200 R 62928 CDS YP_934699.1 119899486 4607846 3518714..3519508 1 NC_008702.1 ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived; thymidylate synthase 3519508 thyA 4607846 thyA Azoarcus sp. BH72 thymidylate synthase YP_934699.1 3518714 D 62928 CDS YP_934700.1 119899487 4609946 3519505..3519999 1 NC_008702.1 Dihydrofolate reductase. catalytic activity: 5,6,7,8-tetrahydrofolate + nadp(+) = 7,8- dihydrofolate + nadph. pathway: essential step for de novo glycine and purine synthesis, dna precursor synthesis, and for the conversion of dump to dtmp. Pfam: Dihydrofolate reductase; High confidence in function and specificity; FolA protein 3519999 folA 4609946 folA Azoarcus sp. BH72 FolA protein YP_934700.1 3519505 D 62928 CDS YP_934701.1 119899488 4608817 complement(3520046..3522289) 1 NC_008702.1 Putative orn/arg/lys decarboxylase. Homology to adiA of E. coli of 37% (sprot|ADIA_ECOLI). This family is composed of ornithine decarboxylases (ODC), arginine decarboxylases (ADC) and lysine decarboxylases (LDC), and belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase domain superfamily (fold I). These enzymes catalyse the decarboxylation of ornithine,arginine, or lysine, respectively using PLP as a co-factor. Pfam: Orn/Lys/Arg decarboxylase no signal peptide no TMHs; Specificity unclear; putative Orn/Arg/Lys decarboxylase 3522289 adiA 4608817 adiA Azoarcus sp. BH72 putative Orn/Arg/Lys decarboxylase YP_934701.1 3520046 R 62928 CDS YP_934702.1 119899489 4608281 complement(3522456..3523022) 1 NC_008702.1 Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis; deoxycytidine triphosphate deaminase 3523022 dcd 4608281 dcd Azoarcus sp. BH72 deoxycytidine triphosphate deaminase YP_934702.1 3522456 R 62928 CDS YP_934703.1 119899490 4608589 complement(3523125..3524216) 1 NC_008702.1 Mrp protein homolog InterPro: Domain of unknown function DUF59 desulf_FeS4: desulfoferrodoxin FeS4 iron; Function unclear; putative iron sulfur binding protein 3524216 4608589 azo3201 Azoarcus sp. BH72 putative iron sulfur binding protein YP_934703.1 3523125 R 62928 CDS YP_934704.1 119899491 4607847 3524441..3525022 1 NC_008702.1 Belongs to the ompa family. InterPro: Bacterial outer membrane protein; Function unclear; lipoprotein attached to the membrane by a lipid anchor 3525022 4607847 azo3202 Azoarcus sp. BH72 lipoprotein attached to the membrane by a lipid anchor YP_934704.1 3524441 D 62928 CDS YP_934705.1 119899492 4607848 3525107..3527278 1 NC_008702.1 methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content; methionyl-tRNA synthetase 3527278 metG 4607848 metG Azoarcus sp. BH72 methionyl-tRNA synthetase YP_934705.1 3525107 D 62928 CDS YP_934706.1 119899493 4607849 3527498..3529189 1 NC_008702.1 Sulfate permease, SulP. Permease member of the MFS superfamily, involved in the transport of sulfate. Putative sulfate transporter ychM. InterPro: IPR002645; STAS. IPR001902; Sulph_transpt. Pfam: PF01740; STAS; 1. PF00916; Sulfate_transp; 1. SignalP: present.; High confidence in function and specificity; putative sulfate transporter 3529189 sulP2 4607849 sulP2 Azoarcus sp. BH72 putative sulfate transporter YP_934706.1 3527498 D 62928 CDS YP_934707.1 119899494 4609909 3529182..3529478 1 NC_008702.1 Conserved hypothetical acylphosphatase. Homology to pf0283 of P. furiosus of 56% (trembl|Q8U414). Acylphosphatase specifically catalyses the hydrolysis of the carboxyl-phosphate bond of acylphosphates, its substrates including 1,3-diphosphoglycerate and carbamyl phosphate. Although its physiological role is as yet unknown it may, however, play a part in the regulation of the glycolytic pathway and pyrimidine biosynthesis. InterPro: Acylphosphatase (IPR001792). Pfam: Acylphosphatase. no signal peptide. no TMHs.; Function unclear; acylphosphatase 3529478 4609909 azo3205 Azoarcus sp. BH72 acylphosphatase YP_934707.1 3529182 D 62928 CDS YP_934708.1 119899495 4607850 complement(3529488..3531545) 1 NC_008702.1 alkaline phosphatase superfamily protein 3531545 4607850 azo3206 Azoarcus sp. BH72 alkaline phosphatase superfamily protein YP_934708.1 3529488 R 62928 CDS YP_934709.1 119899496 4607851 complement(3531569..3532429) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA1688 of Pseudomonas aeruginosa of 54% (trembl|Q9X6R1(SRS)). No domains predicted. Signal p reporting signal peptide present. NO TMH present.; Conserved hypothetical protein; hypothetical protein 3532429 4607851 azo3207 Azoarcus sp. BH72 hypothetical protein YP_934709.1 3531569 R 62928 CDS YP_934710.1 119899497 4607852 complement(3532537..3536055) 1 NC_008702.1 DNA polymerase III alpha subunit. DNA POLYMERASE III IS A COMPLEX MULTICHAIN ENZYME RESPONSIBLE FOR MOST OF THE REPLICATIVE SYNTHESIS IN BACTERIA. THIS DNA POLYMERASE ALSO EXHIBITS 3 TO 5 EXONUCLEASE ACTIVITY. THE ALPHA CHAIN IS THE DNA POLYMERASE (BY SIMILARITY). InterPro: DNA polymerase III alpha subunit TIGRFAM: polc: DNA polymerase III alpha subunit; High confidence in function and specificity; DNA-directed DNA polymerase 3536055 dnaE1 4607852 dnaE1 Azoarcus sp. BH72 DNA-directed DNA polymerase YP_934710.1 3532537 R 62928 CDS YP_934711.1 119899498 4608632 complement(3536117..3537091) 1 NC_008702.1 TREMBL:Q8XXD1: 69% identity, 76% similarity. Quinone oxidoreductase (EC 1.6.5.5) . qor, RSc2184; probable NADPH:quinone reductase, zeta-crystallin homolog oxidoreductase [EC:1.6.5.5] InterPro: Zinc-containing alcohol dehydrogenase superfamily InterPro:IPR002085; Adh_zn_family. IPR002364:QOR_zeta_crystal. Pfam:PF00107; ADH_zinc_N; 1 tdh: L-threonine 3-dehydrogenase; High confidence in function and specificity; NADPH:quinone reductase 3537091 qor 4608632 qor Azoarcus sp. BH72 NADPH:quinone reductase YP_934711.1 3536117 R 62928 CDS YP_934712.1 119899499 4609683 complement(3537116..3538288) 1 NC_008702.1 Exported protein with C-terminal domain; Function unclear; hypothetical protein 3538288 4609683 azo3210 Azoarcus sp. BH72 hypothetical protein YP_934712.1 3537116 R 62928 CDS YP_934713.1 119899500 4607853 complement(3538418..3540706) 1 NC_008702.1 NADP-dependent; catalyzes the oxidative decarboxylation of malate to form pyruvate; decarboxylates oxaloacetate; malic enzyme 3540706 maeB2 4607853 maeB2 Azoarcus sp. BH72 malic enzyme YP_934713.1 3538418 R 62928 CDS YP_934714.1 119899501 4609193 3541054..3541833 1 NC_008702.1 Sporulation initiation inhibitor protein soj. INHIBITS THE INITIATION OF SPORULATION SPO0J ANTAGONIZES THIS INHIBITION. SOJ ULTIMATELY INHIBITS THE ACTIVATION (PHOSPHORYLATION) OF SPO0A. IT IS NOT REQUIRED FOR CHROMOSOME PARTITIONING. InterPro: ParA family ATPase; Function unclear; ParA family protein 3541833 parA3 4609193 parA3 Azoarcus sp. BH72 ParA family protein YP_934714.1 3541054 D 62928 CDS YP_934715.1 119899502 4609499 complement(3541853..3544072) 1 NC_008702.1 DNA helicase II (EC 3.6.1.-). HAS BOTH ATPASE AND HELICASE ACTIVITIES. UNWINDS DNA DUPLEXES WITH 3 TO 5 POLARITY WITH RESPECT TO THE BOUND STRAND AND INITIATES UNWINDING MOST EFFECTIVELY WHEN A SINGLE-STRANDED REGION IS PRESENT. INVOLVED IN THE POSTINCISION EVENTS OF NUCLEOTIDE EXCISION REPAIR AND METHYL-DIRECTED MISMATCH REPAIR. InterPro: UvrD/REP helicase.; High confidence in function and specificity; DNA helicase II 3544072 uvrD 4609499 uvrD Azoarcus sp. BH72 DNA helicase II YP_934715.1 3541853 R 62928 CDS YP_934716.1 119899503 4610084 3544402..3544728 1 NC_008702.1 Conserved hypothetical. Homology to Daro03003519 of Dechloromonas aromatica of 32% (gi|41722969|ref|ZP_00149935.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 3544728 4610084 azo3214 Azoarcus sp. BH72 hypothetical protein YP_934716.1 3544402 D 62928 CDS YP_934717.1 119899504 4607854 3544948..3547569 1 NC_008702.1 leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase; leucyl-tRNA synthetase 3547569 leuS 4607854 leuS Azoarcus sp. BH72 leucyl-tRNA synthetase YP_934717.1 3544948 D 62928 CDS YP_934718.1 119899505 4607855 3547581..3548096 1 NC_008702.1 rare lipoprotein B precursor; putative lipoprotein 3548096 rlpB 4607855 rlpB Azoarcus sp. BH72 putative lipoprotein YP_934718.1 3547581 D 62928 CDS YP_934719.1 119899506 4609730 3548093..3549103 1 NC_008702.1 required for the assembly and function of the DNAX complex which is required for the assembly of the beta subunit onto primed DNA; DNA polymerase III subunit delta 3549103 holA 4609730 holA Azoarcus sp. BH72 DNA polymerase III subunit delta YP_934719.1 3548093 D 62928 CDS YP_934720.1 119899507 4609012 3549209..3550468 1 NC_008702.1 Catalyzes the phosphorylation of L-glutamate during the proline biosynthesis pathway; gamma-glutamyl phosphate reductase 3550468 proA 4609012 proA Azoarcus sp. BH72 gamma-glutamyl phosphate reductase YP_934720.1 3549209 D 62928 CDS YP_934721.1 119899508 4609632 3550691..3551608 1 NC_008702.1 Tyrosine recombinase xerD. Site-specific tyrosine recombinase which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity). InterPro: Phage integrase.; High confidence in function and specificity; integrase/recombinase 3551608 xerD 4609632 xerD Azoarcus sp. BH72 integrase/recombinase YP_934721.1 3550691 D 62928 CDS YP_934722.1 119899509 4610128 3551633..3552124 1 NC_008702.1 Putative DNA-dependent transcriptional regulator,YbaK.68% identity (75% similarity) to TrEMBL;Q748B9. TrEMBL; Q8FK79(29% identity to E.coli,ybaK).SwissProt;P37175 Has PF04073, YbaK / prolyl-tRNA synthetases associated domain;IPR007214; This domain of unknown function is found in numerous prokaryote organisms. The structure of YbaK shows a novel fold. This domain also occurs in a number of prolyl-tRNA synthetases (proRS) from prokaryotes. Thus, the domain is thought to be involved in oligo-nucleotide binding, with possible roles in recognition/discrimination or editing of prolyl-tRNA. TIGR00011; YbaK_EbsC; 1; High confidence in function and specificity; putative transcriptional regulator 3552124 ybaK 4610128 ybaK Azoarcus sp. BH72 putative transcriptional regulator YP_934722.1 3551633 D 62928 CDS YP_934723.1 119899510 4610143 3552261..3552620 1 NC_008702.1 Putative trans-acting regulatory protein HvrA. Homology to hvrA of R. capsulatus of 28% (sprot|HVRA_RHOCA) IT IS A DIM-LIGHT TRANS-ACTING ACTIVATOR OF PUF AND PUH EXPRESSION BUT HAS NO EFFECT ON THE EXPRESSION OF THE PUC OPERON. IT IS RESPONSIBLE FOR REGULATING LIGHT-HARVESTING-I AND REACTION CENTER STRUCTURAL GENE EXPRESSION DIFFERENTIALLY FROM THAT OF LIGHT-HARVESTING-II EXPRESSION IN RESPONSE TO ALTERATIONS IN LIGHT. PROPER LIGHT REGULATION OF LIGHT-HARVESTING AND REACTION CENTER POLYPEPTIDE SYNTHESIS IS INDEED AN IMPORTANT PHYSIOLOGICAL TRAIT THAT ENABLES CELLS TO ADAPT TO EVER-CHANGING ENVIRONMENTAL CONDITIONS OF LIGHT INTENSITY. InterPro: H-NS histone family (IPR001801) Pfam: H-NS histone family no signal peptide no TMHs; Family membership; putative trans-acting regulatory protein HvrA 3552620 hvrA2 4610143 hvrA2 Azoarcus sp. BH72 putative trans-acting regulatory protein HvrA YP_934723.1 3552261 D 62928 CDS YP_934724.1 119899511 4609056 complement(3552711..3553088) 1 NC_008702.1 Dihydroneopterin aldolase (DHNA). CATALYZES THE CONVERSION OF 78-DIHYDRONEOPTERIN TO 6- HYDROXYMETHYL-78-DIHYDROPTERIN. InterPro: Dihydroneopterin aldolase TIGRFAM: folB_dom: FolB domain; High confidence in function and specificity; FolB protein 3553088 folB 4609056 folB Azoarcus sp. BH72 FolB protein YP_934724.1 3552711 R 62928 CDS YP_934725.1 119899512 4608818 3553201..3553797 1 NC_008702.1 Conserved hypothetical membrane protein.51% identity to TrEMBL;Q6D157. Has PF02660, Domain of unknown function DUF;IPR003811; This family consists of hypothetical transmembrane proteins none of which have any known function, the aligned region is around 200 amino acids long. TMHMM2 predicts 4 TMH's. Signal peptide present, but is overlapping with the DYF,PF02660 domain. SO is the TMH's.; Conserved hypothetical protein; hypothetical protein 3553797 4608818 azo3223 Azoarcus sp. BH72 hypothetical protein YP_934725.1 3553201 D 62928 CDS YP_934726.1 119899513 4607856 complement(3553798..3554829) 1 NC_008702.1 in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity; putative DNA-binding/iron metalloprotein/AP endonuclease 3554829 gcp 4607856 gcp Azoarcus sp. BH72 putative DNA-binding/iron metalloprotein/AP endonuclease YP_934726.1 3553798 R 62928 CDS YP_934727.1 119899514 4608867 3554961..3555173 1 NC_008702.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 3555173 rpsU 4608867 rpsU Azoarcus sp. BH72 30S ribosomal protein S21 YP_934727.1 3554961 D 62928 CDS YP_934728.1 119899515 4609824 3555250..3557070 1 NC_008702.1 DNA primase (EC 2.7.7.-). DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis. MG010: DNA primase-related protein.; High confidence in function and specificity; DNA primase 3557070 dnaG 4609824 dnaG Azoarcus sp. BH72 DNA primase YP_934728.1 3555250 D 62928 CDS YP_934729.1 119899516 4608633 3557120..3559108 1 NC_008702.1 sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this is the primary sigma factor of bacteria; RNA polymerase sigma factor RpoD 3559108 rpoD 4608633 rpoD Azoarcus sp. BH72 RNA polymerase sigma factor RpoD YP_934729.1 3557120 D 62928 CDS YP_934730.1 119899517 4608239 complement(3559582..3559950) 1 NC_008702.1 Glyoxalase I catalyzes the first step of the glyoxal pathway. S-lactoylglutathione is then converted by glyoxalase II to lactic acid. Similar to trembl|Q9HY85 (74%) and to sprot|LGUL_NEIMA (70%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily ProDom (PD002334): Glyoxalase I; High confidence in function and specificity; lactoylglutathione lyase 3559950 gloA2 4608239 gloA2 Azoarcus sp. BH72 lactoylglutathione lyase YP_934730.1 3559582 R 62928 CDS YP_934731.1 119899518 4608908 complement(3559972..3560691) 1 NC_008702.1 Probable Hydrogenase expression/formation protein hupH. SIMILARITY:Belongs to the hupH/hyaF family SPROT:P48340: 37% identity, 54% similarity InterPro: IPR002725; DUF45. Pfam: PF01863; DUF45 Nonsecretory protein (Signal P predicted) No transmembrane helices (TMHMM predicted); High confidence in function and specificity; putative hydrolase 3560691 4608908 azo3229 Azoarcus sp. BH72 putative hydrolase YP_934731.1 3559972 R 62928 CDS YP_934732.1 119899519 4607857 complement(3560730..3561479) 1 NC_008702.1 Probable Hypothetical protein Ta0487. trembl:Q7W4W1:50% identity, 62% similarity InterPro:IPR001453; MoCF_biosynth. Pfam: PF00994; MoCF_biosynth; 1. Eukaryotic and prokaryotic molybdoenzymes require a molybdopterin cofactor (MoCF) for their activity. The biosynthesis of this cofactor involves a complex multistep enzymatic pathway. One of the eukaryotic proteins involved in this pathway is the Drosophila protein cinnamon molyb_syn: molybdenum cofactor synthe No signal peptide. No transmembrane helices; Function unclear; hypothetical protein 3561479 4607857 azo3230 Azoarcus sp. BH72 hypothetical protein YP_934732.1 3560730 R 62928 CDS YP_934733.1 119899520 4607858 complement(3561476..3562246) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA4352 of Azoarcus sp. EbN1 of 31% (gnl|keqq|eba:ebA4352(KEGG)). Has PF04401, Protein of unknown function (DUF540); IPR007496 DUF540; Uncharacterised bacterial integral membrane protein,possibly involved in cysteine biosynthesis. Speculated to be involved in sulphate transport. no signal peptide. 6 TMHs; Conserved hypothetical protein; hypothetical protein 3562246 4607858 azo3231 Azoarcus sp. BH72 hypothetical protein YP_934733.1 3561476 R 62928 CDS YP_934734.1 119899521 4607859 complement(3562254..3563612) 1 NC_008702.1 Signal recognition particle protein (Fifty-four homolog) (P48). NECESSARY FOR EFFICIENT EXPORT OF EXTRA-CYTOPLASMIC PROTEINS. BINDS TO THE SIGNAL SEQUENCE WHEN IT EMERGES FROM THE RIBOSOMES.The protein has a two domain structure: the G-domain binds GTP; the M-domain binds the RNA and also binds the signal sequence. It belongs to the GTP-binding SRP family. InterPro: Signal recognition particle protein 3a0501s01: signal recognition particl Pfam: SRP54-type protein (GTPase domain); signal peptide binding domain no TMHs and signal peptide; High confidence in function and specificity; signal recognition particle protein 3563612 ffh 4607859 ffh Azoarcus sp. BH72 signal recognition particle protein YP_934734.1 3562254 R 62928 CDS YP_934735.1 119899522 4608759 3563665..3564489 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ne1459 of N. europaea of 49% (trembl|Q82UM4). Pfam: Cytochrome C assembly protein. no signal peptide. 8 TMHs; Conserved hypothetical protein; hypothetical protein 3564489 4608759 azo3233 Azoarcus sp. BH72 hypothetical protein YP_934735.1 3563665 D 62928 CDS YP_934736.1 119899523 4607860 3564593..3566311 1 NC_008702.1 Pilus assembly protein PilB is a component of the pilus biogenesis apparatus,; High confidence in function and specificity; pilus biogenesis protein 3566311 pilB2 4607860 pilB2 Azoarcus sp. BH72 pilus biogenesis protein YP_934736.1 3564593 D 62928 CDS YP_934737.1 119899524 4609556 3566331..3567566 1 NC_008702.1 Pilus assembly protein PilC is a component of the pilus biogenesis apparatus, similarity to pir|B35384 (50%). This apparatus is responsible for the stabilization, translocation and assembly of the monomeric pilin subunits into the pilus organelle. InterPro (IPR001992): Bacterial type II secretion system protein. InterPro (IPR003004): Bacterial general secretion pathway protein F Pfam (PF00482): Bacterial type II secretion system protein TMHMM reporting three TMH; High confidence in function and specificity; type 4 pilus biogenesis protein 3567566 pilC 4609556 pilC Azoarcus sp. BH72 type 4 pilus biogenesis protein YP_934737.1 3566331 D 62928 CDS YP_934738.1 119899525 4609557 3567612..3568463 1 NC_008702.1 PilD is a prepilin peptidase processes the N-terminus of the prepilins. The processing is essential for the correct formation of the pseudopili of type IV bacterial protein secretion. Similar to pir|A39131 (58%). InterPro(PF01478): Prepilin cysteine protease (C20), type IV InterPro (PR00864): Prepilin cysteine protease (C20),type IV TMHMM reporting six TMH.; High confidence in function and specificity; prepilin peptidase 3568463 pilD 4609557 pilD Azoarcus sp. BH72 prepilin peptidase YP_934738.1 3567612 D 62928 CDS YP_934739.1 119899526 4609558 3568611..3568871 1 NC_008702.1 Conserved hypothetical protein. Homology to BB0113 of B.bronchiseptica of 56% (trembl:Q7WR44). No domas predicted. No TMHs. No signal peptide.; hypothetical protein 3568871 4609558 azo3237 Azoarcus sp. BH72 hypothetical protein YP_934739.1 3568611 D 62928 CDS YP_934740.1 119899527 4607861 3568884..3569510 1 NC_008702.1 Conserved hypothetical membrane protein, 57% identity to TrEMBL;Q7W002. Has PF04367, Protein of unknown function (DUF502);IPR007462; Predicted to be an integral membrane protein. Signal Peptide present. 1 TMHs.; Conserved hypothetical protein; hypothetical protein 3569510 4607861 azo3238 Azoarcus sp. BH72 hypothetical protein YP_934740.1 3568884 D 62928 CDS YP_934741.1 119899528 4607862 3569566..3571365 1 NC_008702.1 catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes; aspartyl-tRNA synthetase 3571365 aspS 4607862 aspS Azoarcus sp. BH72 aspartyl-tRNA synthetase YP_934741.1 3569566 D 62928 CDS YP_934742.1 119899529 4608362 3571506..3573158 1 NC_008702.1 Putative response regulator,; Conserved hypothetical protein; response regulator 3573158 4608362 azo3240 Azoarcus sp. BH72 response regulator YP_934742.1 3571506 D 62928 CDS YP_934743.1 119899530 4607863 3573175..3573540 1 NC_008702.1 InterPro: Guanine-specific ribonuclease N1 and T1; Family membership; ribonuclease 3573540 4607863 azo3241 Azoarcus sp. BH72 ribonuclease YP_934743.1 3573175 D 62928 CDS YP_934744.1 119899531 4607864 3573537..3573992 1 NC_008702.1 Conserved hypothetical protein. Homology to XCC2280 of X.campestris of 33% (trembl:Q8P8G2). No domains predicted. No signal Peptide present. No TMH present.; hypothetical protein 3573992 4607864 azo3242 Azoarcus sp. BH72 hypothetical protein YP_934744.1 3573537 D 62928 CDS YP_934745.1 119899532 4607865 3574142..3574567 1 NC_008702.1 Putative small heat shock protein. Homology to hsp18 of C. acetobutylicum of 35% (sprot|HS18_CLOAB) InterPro: Heat shock hsp20 (alpha crystallin) proteins family (IPR002068) Pfam: Hsp20/alpa cystallin family no signal peptide no TMHs; Function unclear; putative small heat shock protein 3574567 hsp18 4607865 hsp18 Azoarcus sp. BH72 putative small heat shock protein YP_934745.1 3574142 D 62928 CDS YP_934746.1 119899533 4609038 3574680..3575582 1 NC_008702.1 catalyzes the phosphorylation of N-acetyl-L-glutamate to form N-acetyl-L-glutamate 5-phosphate; acetylglutamate kinase 3575582 argB 4609038 argB Azoarcus sp. BH72 acetylglutamate kinase YP_934746.1 3574680 D 62928 CDS YP_934747.1 119899534 4608332 3575587..3576747 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA4328 of Azoarcus sp. EbN1 of 59% (gnl|keqq|eba:ebA4328(KEGG)). no domains predicted. signal peptide. TMH in signal peptide; hypothetical protein 3576747 4608332 azo3245 Azoarcus sp. BH72 hypothetical protein YP_934747.1 3575587 D 62928 CDS YP_934748.1 119899535 4607866 3576895..3578475 1 NC_008702.1 Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active coa thioesters for subsequent degradation or incorporation into phospholipids, TREMBL:Q8UET3 (39% identity); TREMBL:Q988H2 (38% identity). InterPro (IPR000873): AMP-dependent synthetase and ligase Pfam (PF00501): AMP-binding enzyme.; High confidence in function and specificity; long-chain-fatty-acid-CoA ligase 3578475 fadD4 4607866 fadD4 Azoarcus sp. BH72 long-chain-fatty-acid-CoA ligase YP_934748.1 3576895 D 62928 CDS YP_934749.1 119899536 4608725 3578572..3580368 1 NC_008702.1 Asparagine synthase catalyze the conversion of aspartate to asparagine. Similar to trembl|Q82VG1 (45%) and to trembl|Q7USD1 (27%). Pfam (PF00733): Asparagine synthase; Family membership; asparagine synthase 3580368 asn 4608725 asn Azoarcus sp. BH72 asparagine synthase YP_934749.1 3578572 D 62928 CDS YP_934750.1 119899537 4608358 3580380..3581663 1 NC_008702.1 Diaminopimelate decarboxylase,; Specificity unclear; diaminopimelate decarboxylase 3581663 lysA1 4608358 lysA1 Azoarcus sp. BH72 diaminopimelate decarboxylase YP_934750.1 3580380 D 62928 CDS YP_934751.1 119899538 4609184 complement(3581684..3582433) 1 NC_008702.1 Conserved hypothetical secreted protein. Homolgy to ne2159 of N. europaea of 33% (trembl|Q82SY7). InterPro: TPR repeat (IPR001440). Pfam: TPR domain signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 3582433 4609184 azo3249 Azoarcus sp. BH72 hypothetical protein YP_934751.1 3581684 R 62928 CDS YP_934752.1 119899539 4607867 complement(3582430..3583287) 1 NC_008702.1 Conserved hypothetical protein. Homology to ne2161 of N. europaea of 45% (trembl|Q82SY5). Interpro: Esterase/lipase/thioesterase family. no damians predicted. no signal peptide. no TMHs.; hypothetical protein 3583287 4607867 azo3250 Azoarcus sp. BH72 hypothetical protein YP_934752.1 3582430 R 62928 CDS YP_934753.1 119899540 4607868 complement(3583284..3584120) 1 NC_008702.1 Probable Adenylate cyclase (EC 4.6.1.1) (ATP pyrophosphate-lyase) (Adenylyl cyclase). TREMBL:Q82SY4:40% identity, 57% similarity. InterPro:IPR000379; Ser_estrs Pfam:UPF0007:Uncharacterized protein family UPF000 thiE: thiamine-phosphate pyrophosphorylas No transmembrane helices; Function unclear; hypothetical protein 3584120 4607868 azo3251 Azoarcus sp. BH72 hypothetical protein YP_934753.1 3583284 R 62928 CDS YP_934754.1 119899541 4607869 complement(3584127..3584411) 1 NC_008702.1 Conserved hypothetical protein. Homology to ne2163 of N. europaea of 42% (trembl|Q82SY3). Pfam: Poshpopantetheine attachment site. A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. no signal peptide. no TMHS; hypothetical protein 3584411 4607869 azo3252 Azoarcus sp. BH72 hypothetical protein YP_934754.1 3584127 R 62928 CDS YP_934755.1 119899542 4607870 3584536..3585771 1 NC_008702.1 Conserved hypothetical protein. Homology to Mmc102002678 of Magnetococcus sp. MC-1 of 44% (gi|48831833|ref|ZP_00288884.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 3585771 4607870 azo3253 Azoarcus sp. BH72 hypothetical protein YP_934755.1 3584536 D 62928 CDS YP_934756.1 119899543 4607871 3585774..3586460 1 NC_008702.1 May be related to biosynthesos of cell surface carbohydrates / polysaccharides.; hypothetical protein 3586460 4607871 azo3254 Azoarcus sp. BH72 hypothetical protein YP_934756.1 3585774 D 62928 CDS YP_934757.1 119899544 4607872 3586547..3588568 1 NC_008702.1 Specificity unclear; acyltransferase 3588568 4607872 azo3255 Azoarcus sp. BH72 acyltransferase YP_934757.1 3586547 D 62928 CDS YP_934758.1 119899545 4607873 3588679..3590055 1 NC_008702.1 Conserved hypothetical polysaccharide biosynthesis protein, related to GumJ. Homology to ebA4295 of Azoarcus sp. EbN1 of 42%. Pfam: Polysaccharide biosynthesis protein. Members of this family are integral membrane proteins. Many members of the family are implicated in production of polysaccharide. The family includes RfbX part of the O antigen biosynthesis operon. The family includes SpoVB from Bacillus subtilis SP5B_BACSU, which is involved in spore cortex biosynthesis. No signal peptide. 10 TMHs.; Family membership; hypothetical protein 3590055 4607873 azo3256 Azoarcus sp. BH72 hypothetical protein YP_934758.1 3588679 D 62928 CDS YP_934759.1 119899546 4607874 3590109..3591152 1 NC_008702.1 Similar to putative exopolysaccharide polymerization protein PssK; Family membership; polysaccharide polymerase 3591152 4607874 azo3257 Azoarcus sp. BH72 polysaccharide polymerase YP_934759.1 3590109 D 62928 CDS YP_934760.1 119899547 4607875 3591156..3592049 1 NC_008702.1 InterPro: Glycosyl transferase family 2; Function unclear; glycosyltransferase 3592049 4607875 azo3258 Azoarcus sp. BH72 glycosyltransferase YP_934760.1 3591156 D 62928 CDS YP_934761.1 119899548 4607876 complement(3592004..3592930) 1 NC_008702.1 InterPro: Glycosyl transferase family 2; Specificity unclear; glycosyltransferase 3592930 4607876 azo3259 Azoarcus sp. BH72 glycosyltransferase YP_934761.1 3592004 R 62928 CDS YP_934762.1 119899549 4607877 3593146..3594084 1 NC_008702.1 InterPro: Glycosyl transferase family 2; Specificity unclear; glycosyltransferase 3594084 4607877 azo3260 Azoarcus sp. BH72 glycosyltransferase YP_934762.1 3593146 D 62928 CDS YP_934763.1 119899550 4607878 3594077..3595309 1 NC_008702.1 Putative glycosyl transferase ypjH (EC 2.-.-.-). TIGR00022: conserved hypothetical pro; Specificity unclear; glycosyltransferase 3595309 4607878 azo3261 Azoarcus sp. BH72 glycosyltransferase YP_934763.1 3594077 D 62928 CDS YP_934764.1 119899551 4607879 3595306..3596340 1 NC_008702.1 Hypothetical glycosyl transferase MJ1607 (EC 2.-.-.-). InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3596340 4607879 azo3262 Azoarcus sp. BH72 glycosyltransferase YP_934764.1 3595306 D 62928 CDS YP_934765.1 119899552 4607880 3596367..3597584 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. signal peptide present.; hypothetical protein 3597584 4607880 azo3263 Azoarcus sp. BH72 hypothetical protein YP_934765.1 3596367 D 62928 CDS YP_934766.1 119899553 4607881 complement(3597597..3599087) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology RB4730 of Rhodopirellula baltica of 36% (trembl|Q7US36). No domains predicted. Signal peptide. No TMHs; Conserved hypothetical protein; hypothetical protein 3599087 4607881 azo3264 Azoarcus sp. BH72 hypothetical protein YP_934766.1 3597597 R 62928 CDS YP_934767.1 119899554 4607882 complement(3599098..3600003) 1 NC_008702.1 Similar to a gene annotaed to encode a dolichol-phosphate mannosyltransferase, a glycosyltransferase that transfers a suga directly to a lipid carrier. InterPro: Glycosyl transferase family 2; Specificity unclear; glycosyltransferase 3600003 4607882 azo3265 Azoarcus sp. BH72 glycosyltransferase YP_934767.1 3599098 R 62928 CDS YP_934768.1 119899555 4607883 complement(3600066..3601037) 1 NC_008702.1 Conserved hypothetical polysacchacride deacetylase. Homology to N. europaea of N. europaea of 54% (trembl|Q82W49(SRS)). This domain is found in polysaccharide deacetylase. This family of polysaccharide deacetylases includes NodB (nodulation protein B from Rhizobium) which is a chitooligosaccharide deacetylase. It also includes chitin deacetylase from yeast, and endoxylanases which hydrolyses glucosidic bonds in xylan. InterPro:IPR002509; Polysac_deacet. Pfam:PF01522; Polysacc_deac_1; 1. no TMHs no signal peptide; Conserved hypothetical protein; polysaccharide deacetylase 3601037 4607883 azo3266 Azoarcus sp. BH72 polysaccharide deacetylase YP_934768.1 3600066 R 62928 CDS YP_934769.1 119899556 4607884 complement(3601034..3602380) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RferDRAFT_3275 of Rhodoferax ferrireducens of 46%. Pfam: O-Antigen Polymerase. This group of bacterial proteins is involved in the synthesis of O-antigen, a lipopolysaccharide found in the outer membrane in gram-negative bacteria. The enzyme is coded for by the gene wzy which is part of the O-antigen gene cluster. No signal peptide. 9 TMHs.; Family membership; hypothetical protein 3602380 4607884 azo3267 Azoarcus sp. BH72 hypothetical protein YP_934769.1 3601034 R 62928 CDS YP_934770.1 119899557 4607885 complement(3602380..3603606) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3603606 4607885 azo3268 Azoarcus sp. BH72 glycosyltransferase YP_934770.1 3602380 R 62928 CDS YP_934771.1 119899558 4607886 complement(3603654..3604970) 1 NC_008702.1 [EC:1.1.1.132] , UDP-glucose 6-dehydrogenase (EC 1.1.1.22) (UDP-Glc dehydrogenase) (UDP-GlcDH) (UDPGDH).,UDP-glucose/GDP-mannose dehydrogenase, UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain; High confidence in function and specificity; GDP-mannose 6-dehydrogenase 3604970 algD 4607886 algD Azoarcus sp. BH72 GDP-mannose 6-dehydrogenase YP_934771.1 3603654 R 62928 CDS YP_934772.1 119899559 4608296 complement(3604967..3606178) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3606178 4608296 azo3270 Azoarcus sp. BH72 glycosyltransferase YP_934772.1 3604967 R 62928 CDS YP_934773.1 119899560 4607887 complement(3606175..3608103) 1 NC_008702.1 Asparagine synthetase B catalyzes the assembly of asparagine from aspartate, Mg(2+)ATP, and glutamine. Similar to trembl|Q7USD1 (35%) and to sprot|ASNB_BACSU (30%). Pfam (PF00310): Glutamine amidotransferase class-II Pfam (PF00733): Asparagine synthase; Specificity unclear; asparagine synthetase 3608103 asnB1 4607887 asnB1 Azoarcus sp. BH72 asparagine synthetase YP_934773.1 3606175 R 62928 CDS YP_934774.1 119899561 4608359 complement(3608105..3609364) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3609364 4608359 azo3272 Azoarcus sp. BH72 glycosyltransferase YP_934774.1 3608105 R 62928 CDS YP_934775.1 119899562 4607888 complement(3609333..3611267) 1 NC_008702.1 Asparagine synthetase B catalyzes the assembly of asparagine from aspartate, Mg(2+)ATP, and glutamine. Similar to trembl|Q81ZU7 (64%) and to trembl|Q7USD1 (37%). Pfam (PF00310): Glutamine amidotransferase class-II Pfam (PF00733): Asparagine synthase; Specificity unclear; asparagine synthetase 3611267 asnB2 4607888 asnB2 Azoarcus sp. BH72 asparagine synthetase YP_934775.1 3609333 R 62928 CDS YP_934776.1 119899563 4608360 complement(3611287..3612462) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3612462 4608360 azo3274 Azoarcus sp. BH72 glycosyltransferase YP_934776.1 3611287 R 62928 CDS YP_934777.1 119899564 4607889 complement(3612459..3614009) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to NE1797 of Nitrosomonas europaea of 33% (trembl|Q82TS7(SRS)). no domains predicted. signal peptide. 7 TMHs; Conserved hypothetical protein; hypothetical protein 3614009 4607889 azo3275 Azoarcus sp. BH72 hypothetical protein YP_934777.1 3612459 R 62928 CDS YP_934778.1 119899565 4607890 complement(3614065..3615168) 1 NC_008702.1 Possibly related to cell surface polysaccharide biosynthesis, due to the presence of the gene in a polysaccharide synthesis gene cluster.; Function unclear; hypothetical protein 3615168 4607890 azo3276 Azoarcus sp. BH72 hypothetical protein YP_934778.1 3614065 R 62928 CDS YP_934779.1 119899566 4607891 complement(3615137..3615997) 1 NC_008702.1 62% Myb_DNA_binding.IPR002509; Polysac_deacet. Pfam:PF01522; Polysacc_deac_1; 1.; hypothetical protein 3615997 4607891 azo3277 Azoarcus sp. BH72 hypothetical protein YP_934779.1 3615137 R 62928 CDS YP_934780.1 119899567 4607892 complement(3615997..3617154) 1 NC_008702.1 UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14) (UDP-GlcNAc-2- epimerase). Catalyzes the reversible epimerization at C-2 of UDP-N- acetylglucosamine (UDP-GlcNAc) and thereby provides bacteria with UDP-N-acetylmannosamine (UDP-ManNAc) the activated donor of ManNAc residues (By similarity). InterPro: UDP-N-acetylglucosamine 2-epimerase; High confidence in function and specificity; UDP-N-acetylglucosamine 2-epimerase 3617154 mnaA 4607892 mnaA Azoarcus sp. BH72 UDP-N-acetylglucosamine 2-epimerase YP_934780.1 3615997 R 62928 CDS YP_934781.1 119899568 4609232 complement(3617151..3618224) 1 NC_008702.1 General secretion pathway protein A. Involved in a general secretion pathway (GSP) for the export of proteins. InterPro: AAA ATPase superfamily POssibly related to the biosynthesis of cell surface polysaccharide due to the presence of the gene in a polysaccharide synthesis gene cluster.; High confidence in function and specificity; MSHA biogenesis protein MshM 3618224 gspA 4609232 gspA Azoarcus sp. BH72 MSHA biogenesis protein MshM YP_934781.1 3617151 R 62928 CDS YP_934782.1 119899569 4608946 complement(3618237..3619733) 1 NC_008702.1 hypothetical protein 3619733 4608946 azo3280 Azoarcus sp. BH72 hypothetical protein YP_934782.1 3618237 R 62928 CDS YP_934783.1 119899570 4607893 complement(3619774..3620715) 1 NC_008702.1 Putative tyrosine-protein kinase (EC 2.7.10.1).; High confidence in function and specificity; protein-tyrosine kinase 3620715 wzc2 4607893 wzc2 Azoarcus sp. BH72 protein-tyrosine kinase YP_934783.1 3619774 R 62928 CDS YP_934784.1 119899571 4610119 complement(3620729..3622273) 1 NC_008702.1 Conserved hypothetical polysaccharide chain length determinant protein. Homology to ebA4249 of Azoarcus sp. EbN1 of 66%. No signal peptide. 3 TMHS. Pfam: Wzz = Chain length determinant protein. This family includes proteins involved in lipopolysaccharide (lps) biosynthesis. This family comprises the whole length of chain length determinant protein (or wzz protein) that confers a modal distribution of chain length on the O-antigen component of lps. This region is also found as part of bacterial tyrosine kinases such as ETK_ECOLI.; Family membership; polysaccharide chain length determinant protein 3622273 4610119 azo3282 Azoarcus sp. BH72 polysaccharide chain length determinant protein YP_934784.1 3620729 R 62928 CDS YP_934785.1 119899572 4607894 complement(3622352..3622981) 1 NC_008702.1 Conserved hypothetical polysaccharid export protein. Homology to wza of Azoarcus sp. EbN1 of 80%. Pfam: Polysaccharide biosynthesis/export protein. This is a family of periplasmic proteins involved in polysaccharide biosynthesis and/or export. No TMHs. Signal peptide present., ,; Family membership; polysaccharide export protein 3622981 wza 4607894 wza Azoarcus sp. BH72 polysaccharide export protein YP_934785.1 3622352 R 62928 CDS YP_934786.1 119899573 4610117 3623219..3623971 1 NC_008702.1 Conserved hypothetical protein. Homology to ne2229 of N. europaea of 38% (trembl|Q82SS7). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 3623971 4610117 azo3284 Azoarcus sp. BH72 hypothetical protein YP_934786.1 3623219 D 62928 CDS YP_934787.1 119899574 4607895 complement(3623979..3625292) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NE2243 of Nitrosomonas europaea of 43% (trembl|Q82SR4(SRS). No domains predicted. Signal peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 3625292 4607895 azo3285 Azoarcus sp. BH72 hypothetical protein YP_934787.1 3623979 R 62928 CDS YP_934788.1 119899575 4607896 complement(3625296..3626045) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs. Similar to TREMBL:Q82SR3 (67% identity); TREMBL:Q92NU9 (48% identity); SWISSPROT:Q58206 (45% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR003593): AAA ATPase. Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; Family membership; ABC transporter ATP-binding protein 3626045 4607896 azo3286 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934788.1 3625296 R 62928 CDS YP_934789.1 119899576 4607897 3626090..3626872 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NE2245 of Nitrosomonas europaea of 51% (tremble:Q82SR2). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3626872 4607897 azo3287 Azoarcus sp. BH72 hypothetical protein YP_934789.1 3626090 D 62928 CDS YP_934790.1 119899577 4607898 3626917..3627633 1 NC_008702.1 Putative exonuclease of the beta-lactamase fold involved in RNA processing, N-terminal fragment.; Family membership; RNA procession exonuclease 3627633 4607898 azo3288 Azoarcus sp. BH72 RNA procession exonuclease YP_934790.1 3626917 D 62928 CDS YP_934791.1 119899578 4607899 3627672..3628322 1 NC_008702.1 Putative exonuclease of the beta-lactamase fold involved in RNA processing, C-terminal fragment; Family membership; RNA procession exonuclease 3628322 4607899 azo3289 Azoarcus sp. BH72 RNA procession exonuclease YP_934791.1 3627672 D 62928 CDS YP_934792.1 119899579 4607900 complement(3628428..3629525) 1 NC_008702.1 Major outer membrane protein P.IB precursor (Protein IB) (PIB) (Porin). Serves as a slightly cation selective porin. InterPro: General diffusion Gram-negative porins L12: ribosomal protein L7/L12; Function unclear; outer membrane porin protein 3629525 4607900 azo3290 Azoarcus sp. BH72 outer membrane porin protein YP_934792.1 3628428 R 62928 CDS YP_934793.1 119899580 4607901 complement(3629820..3630890) 1 NC_008702.1 Outer membrane porin protein 32 precursor (OMP32). Forms anion selective channels. InterPro: General diffusion Gram-negative porins 2A0310: amino acid permease (yeast); Function unclear; outer membrane porin protein 3630890 4607901 azo3291 Azoarcus sp. BH72 outer membrane porin protein YP_934793.1 3629820 R 62928 CDS YP_934794.1 119899581 4607902 3631171..3632025 1 NC_008702.1 binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters; RNA polymerase factor sigma-32 3632025 rpoH 4607902 rpoH Azoarcus sp. BH72 RNA polymerase factor sigma-32 YP_934794.1 3631171 D 62928 CDS YP_934795.1 119899582 4609799 complement(3632080..3632682) 1 NC_008702.1 SCO2 protein homolog mitochondrial precursor. Acts as a copper chaperone transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2). InterPro: SCO1/SenC TREMBL:Q8Y2G3: 56% identity, 74% similarity InterPro:IPR003782; SCO1_SenC. Pfam: PF02630; SCO1-SenC dsbE: periplasmic protein thiol:disulf Presence of signal peptide (SignalP predicted) No TMH's present; Family membership; SCO1/SenC family protein 3632682 senC 4609799 senC Azoarcus sp. BH72 SCO1/SenC family protein YP_934795.1 3632080 R 62928 CDS YP_934796.1 119899583 4609864 complement(3632697..3633596) 1 NC_008702.1 converts protoheme IX and farnesyl diphosphate to heme O; protoheme IX farnesyltransferase 3633596 coxD 4609864 coxD Azoarcus sp. BH72 protoheme IX farnesyltransferase YP_934796.1 3632697 R 62928 CDS YP_934797.1 119899584 4608523 complement(3633593..3634636) 1 NC_008702.1 Putative Cytochrome aa3 oxidase assembly protein,42% Identity to TrEMBL;Q7P0G1, Q62F60. Has PF02628,Cytochrome oxidase assembly protein; IPR003780,COX15_CtaA; This is a family of integral membrane proteins. CtaA is required for cytochrome aa3 oxidase assembly in Bacillus subtilis. COX15 is required for cytochrome c oxidase assembly in yeast (P40086).; putative cytochrome aa3 oxidase assembly protein 3634636 4608523 azo3295 Azoarcus sp. BH72 putative cytochrome aa3 oxidase assembly protein YP_934797.1 3633593 R 62928 CDS YP_934798.1 119899585 4607903 complement(3634633..3635286) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS03338 of Ralstonia solanacearum of 36% (trembl|Q8Y2G6(SRS)). No domains predicted. No signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 3635286 4607903 azo3296 Azoarcus sp. BH72 hypothetical protein YP_934798.1 3634633 R 62928 CDS YP_934799.1 119899586 4607904 complement(3635261..3635983) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to NE1012 of N.europaea of 31% (tremble:Q82VQ4). No domains predicted. Signal peptide present. No TMHs; Conserved hypothetical protein; hypothetical protein 3635983 4607904 azo3297 Azoarcus sp. BH72 hypothetical protein YP_934799.1 3635261 R 62928 CDS YP_934800.1 119899587 4607905 3636032..3636226 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs0336 of R. solanacearum of 53% (trembl|Q8Y2G8(SRS)). no domains predicted. signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 3636226 4607905 azo3298 Azoarcus sp. BH72 hypothetical protein YP_934800.1 3636032 D 62928 CDS YP_934801.1 119899588 4607906 complement(3636240..3637091) 1 NC_008702.1 Cytochrome c oxidase polypeptide III Subunits I II and III form the functional core of the enzyme complex. InterPro: Cytochrome c oxidase subunit III; High confidence in function and specificity; cytochrome-c oxidase 3637091 coxC 4607906 coxC Azoarcus sp. BH72 cytochrome-c oxidase YP_934801.1 3636240 R 62928 CDS YP_934802.1 119899589 4608522 complement(3637132..3637332) 1 NC_008702.1 Conserved hypothetical membrane protein, 73% similarity to TrEMBL;Q7WE10,Putative membrane protein [BB4828] [Bordetella bronchiseptica(Alcaligenes bronchisepticus). no signal peptide. 1 TMHs; hypothetical protein 3637332 4608522 azo3300 Azoarcus sp. BH72 hypothetical protein YP_934802.1 3637132 R 62928 CDS YP_934803.1 119899590 4607907 complement(3637322..3637897) 1 NC_008702.1 involved in the insertion of copper into subunit I of cytochrome C oxidase; cytochrome C oxidase assembly protein 3637897 coxG 4607907 coxG Azoarcus sp. BH72 cytochrome C oxidase assembly protein YP_934803.1 3637322 R 62928 CDS YP_934804.1 119899591 4608524 complement(3637894..3638010) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc0364 of R. solanacearum of 42% (trembl|Q8Y2H2) no domains predicted no signal peptide 1 TMHs; Conserved hypothetical protein; hypothetical protein 3638010 4608524 azo3302 Azoarcus sp. BH72 hypothetical protein YP_934804.1 3637894 R 62928 CDS YP_934805.1 119899592 4607908 complement(3638035..3639612) 1 NC_008702.1 Probable cytochrome c oxidase, subunit I (EC 1.9.3.1) (Cytochrome AA3 subunit 1). Homology to coxA of B. japonicum of 55% (sprot|COX1_BRAJA(SRS)) cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. subunits 1- 3 form the functional core of the enzyme complex. co i is the catalytic subunit of the enzyme. electrons originating in cytochrome c are transferred via the copper a center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper b. Pfam: Cytochrome c and quinol oxidase polypeptide no signal peptide 12 TMHs; High confidence in function and specificity; cytochrome c oxidase subunit I 3639612 coxA 4607908 coxA Azoarcus sp. BH72 cytochrome c oxidase subunit I YP_934805.1 3638035 R 62928 CDS YP_934806.1 119899593 4608520 complement(3639657..3640799) 1 NC_008702.1 Conserved hypothetical cytochrome c oxidase,subunit II. Homology to bb4831 of B. bronchiseptica of 60% (trembl|Q7WE07). SUBUNIT I AND II FORM THE FUNCTIONAL CORE OF THE ENZYME COMPLEX. ELECTRONS ORIGINATING IN CYTOCHROME C ARE TRANSFERRED VIA HEME A AND CU(A) TO THE BINUCLEAR CENTER FORMED BY HEME A3 AND CU(B). InterPro: Cytochrome c oxidase subunit II (IPR002429); cytochrome c, class IC (IPR008168); cytochrome, class I (IPR003088); Cu(A) centre of cytochrome coxidase, subunit II and nitrous oxide reductase (IPR001505) Pfam: Cytochrome c oxidase subunit II, transmembran domain; cytochrome c oxidase subunit II,periplasmic domain; cytochrome c Tirgfam: ccoP: cytochrome c oxidase cbb3-type signal peptide 2 TMHs; Conserved hypothetical protein; cytochrome c oxidase subunit II 3640799 coxB 4608520 coxB Azoarcus sp. BH72 cytochrome c oxidase subunit II YP_934806.1 3639657 R 62928 CDS YP_934807.1 119899594 4608521 complement(3641189..3641698) 1 NC_008702.1 ADP-heptose synthase (EC 2.7.-.-). cyt_tran_rel: cytidyltransferase-related; High confidence in function and specificity; ADP-heptose synthase 3641698 waaE1 4608521 waaE1 Azoarcus sp. BH72 ADP-heptose synthase YP_934807.1 3641189 R 62928 CDS YP_934808.1 119899595 4610103 complement(3641777..3643381) 1 NC_008702.1 Conserved hypothetical Na+-dependent phosphate transporter protein. Homolg to Bacteroides thetaiotaomicron. TREMBL:Q8A4R9 InterPro:IPR003841; Na/Pi_cotranspt.IPR004633; NaPi_cotransptII. Pfam:PF02690; Na_Pi_cotrans; 1. SignalP: present TMhelix:8 present. InterPro: Na+/Pi-cotransporter NaPi_cotrn_rel: Na/Pi cotransporter II Hypothetical protein yjbB.; Specificity unclear; hypothetical protein 3643381 4610103 azo3306 Azoarcus sp. BH72 hypothetical protein YP_934808.1 3641777 R 62928 CDS YP_934809.1 119899596 4607909 complement(3643550..3645082) 1 NC_008702.1 Probable coenzyme A transferase. Homology to cat1 of C. kluyveri of 51% (sprot|CAT1_CLOKL). Pfam: Acetyl-CoA hydrolase/transferase no signal peptide no TMHs tal_mycobact: transaldolase; Family membership; coenzyme A transferase 3645082 cat1 4607909 cat1 Azoarcus sp. BH72 coenzyme A transferase YP_934809.1 3643550 R 62928 CDS YP_934810.1 119899597 4608420 complement(3645353..3646273) 1 NC_008702.1 Putative MerR-family transcriptional regulator. InterPro: IPR000551 HTH_MerR. HTH reporting nucleic acid binding motif. h_aconitase: homoaconitase; MerR family transcriptional regulator 3646273 4608420 azo3308 Azoarcus sp. BH72 MerR family transcriptional regulator YP_934810.1 3645353 R 62928 CDS YP_934811.1 119899598 4607910 3646563..3647906 1 NC_008702.1 Conserved hypothetical amine oxidoreductase. Homology to atu1977 of A. tumefaciens of 47% (trembl|Q8UDY9). Pfam: Flavine containing amine oxidoreductase no signal peptide 1 TMHs; Conserved hypothetical protein; amine oxidoreductase 3647906 4607910 azo3309 Azoarcus sp. BH72 amine oxidoreductase YP_934811.1 3646563 D 62928 CDS YP_934812.1 119899599 4607911 3647986..3648756 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3225 of C.violaceum of 50% (trembl:Q7NPR5). Has PF07103,Protein of unknown function (DUF1365);IPR010775 ;This family consists of several bacterial and plant proteins of around 250 residues in length. The function of this family is unknown. No signal peptide or TMH present.; hypothetical protein 3648756 4607911 azo3310 Azoarcus sp. BH72 hypothetical protein YP_934812.1 3647986 D 62928 CDS YP_934813.1 119899600 4607912 3648753..3650036 1 NC_008702.1 Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79) (Cyclopropane fatty acid synthase) (CFA synthase). InterPro: Cyclopropane-fatty-acyl-phospholipid synthase; High confidence in function and specificity; cyclopropane-fatty-acyl-phospholipid synthase 3650036 cfaA 4607912 cfaA Azoarcus sp. BH72 cyclopropane-fatty-acyl-phospholipid synthase YP_934813.1 3648753 D 62928 CDS YP_934814.1 119899601 4608463 3650039..3650623 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2729 of P.putida of 36% (tremble:Q88JC0). No domains predicted. Signal peptide present. NO TMH reported present.; Conserved hypothetical protein; hypothetical protein 3650623 4608463 azo3312 Azoarcus sp. BH72 hypothetical protein YP_934814.1 3650039 D 62928 CDS YP_934815.1 119899602 4607913 3650639..3651958 1 NC_008702.1 Integral membrane protein.Belongs to the Na/galactoside symporter family. 20% MFS.IPR001927; Na/Gal_symport. TIGRFAMs:TIGR00792; gph; 1. TMHelix:12.; Function unclear; putative symporter protein 3651958 4607913 azo3313 Azoarcus sp. BH72 putative symporter protein YP_934815.1 3650639 D 62928 CDS YP_934816.1 119899603 4607914 3651955..3652512 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PP2730 of P.putida of 48% (tremble:Q88JB9). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3652512 4607914 azo3314 Azoarcus sp. BH72 hypothetical protein YP_934816.1 3651955 D 62928 CDS YP_934817.1 119899604 4607915 3652517..3653299 1 NC_008702.1 The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases,TREMBL:Q92PV6 (39 % identity); TREMBL:Q88JB2 (33% identity). InterPro (IPR002198): Short-chain dehydrogenase/reductase (SDR). InterPro (IPR002347): Glucose/ribitol dehydrogenase. Pfam (PF00106): Short chain dehydrogenase.; Specificity unclear; short-chain dehydrogenase 3653299 4607915 azo3315 Azoarcus sp. BH72 short-chain dehydrogenase YP_934817.1 3652517 D 62928 CDS YP_934818.1 119899605 4607916 3653304..3653753 1 NC_008702.1 Conserved hypothetical protein. Homology to CV3228 of C.violaceum of 40% (trembl:Q7NT36). No domains predicted. No Signal peptide present. No TMH reported present.; hypothetical protein 3653753 4607916 azo3316 Azoarcus sp. BH72 hypothetical protein YP_934818.1 3653304 D 62928 CDS YP_934819.1 119899606 4607917 complement(3653743..3654726) 1 NC_008702.1 TREMBL:Q881C6:69% identity; 83% similarity. Alcohol dehydrogenase (EC: 1.1.1.1) (ADH) catalyzes the reversible oxidation of ethanol to acetaldehyde with the concomitant reduction of NAD: Ethanol + NAD = Acetaldehyde + NADH Protein yhdH. InterPro:IPR002085; Adh_zn_family. Pfam:PF00107; ADH_zinc_N; FAD binding domain InterPro: Zinc-containing alcohol dehydrogenase superfamily TIGRFAM:tdh: L-threonine 3-dehydrogenase; High confidence in function and specificity; zinc-binding alcohol dehydrogenase 3654726 yhdH 4607917 yhdH Azoarcus sp. BH72 zinc-binding alcohol dehydrogenase YP_934819.1 3653743 R 62928 CDS YP_934820.1 119899607 4610181 3654928..3656775 1 NC_008702.1 ATP-dependent DNA helicase recQ (EC 3.6.1.-). INVOLVED IN THE RECF RECOMBINATION PATHWAY; ITS GENE EXPRESSION IS UNDER THE REGULATION OF THE SOS SYSTEM. IT IS A DNA HELICASE. recq: ATP-dependent DNA helicase RecQ.; High confidence in function and specificity; ATP-dependent DNA helicase 3656775 recQ 4610181 recQ Azoarcus sp. BH72 ATP-dependent DNA helicase YP_934820.1 3654928 D 62928 CDS YP_934821.1 119899608 4609710 complement(3656851..3657195) 1 NC_008702.1 Peptidyl-prolyl cis-trans isomerase. Homology to fkbP of N. meningitidis of 69% (sprot|FKBP_NEIMB) PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. InterPro: FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase)(IPR001179) Pfam: FKBP-type peptidyl-prolyl cis-trans isomerase no signal peptide no TMHs; High confidence in function and specificity; peptidyl-prolyl cis-trans isomerase 3657195 fkbP 4609710 fkbP Azoarcus sp. BH72 peptidyl-prolyl cis-trans isomerase YP_934821.1 3656851 R 62928 CDS YP_934822.1 119899609 4608768 complement(3657267..3657827) 1 NC_008702.1 Putative peptide methionine sulfoxide reductase. Homology to msrA of E. coli of 37% (SWISSPROT:MSRA_ECOLI) Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine (By similarity). InterPro: Peptide methionine sulfoxide reductase (IPR002569) Pfam: Peptide methionine sulfoxide reductase Tigrfam: msrA: peptide methionine sulfoxide reductase no signal peptide no TMHs; Family membership; putative peptide methionine sulfoxide reductase 3657827 msrA 4608768 msrA Azoarcus sp. BH72 putative peptide methionine sulfoxide reductase YP_934822.1 3657267 R 62928 CDS YP_934823.1 119899610 4609280 complement(3657957..3661868) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc2383 of R. solanacearum of 31% (trembl|Q8XWT7(SRS)). no domains predicted .no signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 3661868 4609280 azo3321 Azoarcus sp. BH72 hypothetical protein YP_934823.1 3657957 R 62928 CDS YP_934824.1 119899611 4607918 complement(3661872..3662666) 1 NC_008702.1 involved in tryptophan biosynthesis; amino acid biosynthesis; converts 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide and water; indole-3-glycerol-phosphate synthase 3662666 trpC 4607918 trpC Azoarcus sp. BH72 indole-3-glycerol-phosphate synthase YP_934824.1 3661872 R 62928 CDS YP_934825.1 119899612 4610031 complement(3662666..3663703) 1 NC_008702.1 Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate; anthranilate phosphoribosyltransferase 3663703 trpD 4610031 trpD Azoarcus sp. BH72 anthranilate phosphoribosyltransferase YP_934825.1 3662666 R 62928 CDS YP_934826.1 119899613 4610032 complement(3663719..3664306) 1 NC_008702.1 TrpG; with TrpE catalyzes the formation of anthranilate and glutamate from chorismate and glutamine; TrpG provides the glutamine amidotransferase activity; anthranilate synthase component II 3664306 trpG 4610032 trpG Azoarcus sp. BH72 anthranilate synthase component II YP_934826.1 3663719 R 62928 CDS YP_934827.1 119899614 4610035 complement(3664662..3666134) 1 NC_008702.1 with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine; anthranilate synthase component I 3666134 trpE 4610035 trpE Azoarcus sp. BH72 anthranilate synthase component I YP_934827.1 3664662 R 62928 CDS YP_934828.1 119899615 4610033 complement(3666394..3667065) 1 NC_008702.1 Phosphoglycolate phosphatase 1 (EC 3.1.3.18) (PGP 1). TREMBL:Q7W391: 50% identity, 65% similarity. catalytic activity: 2-phosphoglycolate + h(2)o = glycolate + phosphate. similarity:belongs to the cbby/cbbz/gph/yieh family. InterPro: Haloacid dehalogenase/epoxide hydrolase family InterPro:IPR006402; HAD-SF-IA-v3. IPR006439; HAD_SF_A_v1. IPR005833; Hlgnase/hydrlase. Pfam PF00702; Hydrolase; 1. PRINTS PR00413; HADHALOGNASE. TIGRFAMs:TIGR01549; HAD-SF-IA-v1; 1 hstdl_phs_rel: histidinol phosphatase-r; High confidence in function and specificity; phosphoglycolate phosphatase 3667065 cbbZ 4610033 cbbZ Azoarcus sp. BH72 phosphoglycolate phosphatase YP_934828.1 3666394 R 62928 CDS YP_934829.1 119899616 4608422 complement(3667075..3667776) 1 NC_008702.1 Ribulose-phosphate 3-epimerase(also known as pentose-5-phosphate 3-epimerase, PPE or Rpe), is the enzyme that converts D-ribulose 5-phosphate into D-xylulose 5-phosphate in Calvin's reductive pentose phosphate cycle. 71%Ribul_P_3_epim. Pfam:PF00834; Ribul_P_3_epim; 1. TIGRFAMs:TIGR01163; rpe; 1.; High confidence in function and specificity; putative ribulose-phosphate 3-epimerase 3667776 rpe 4608422 rpe Azoarcus sp. BH72 putative ribulose-phosphate 3-epimerase YP_934829.1 3667075 R 62928 CDS YP_934830.1 119899617 4609758 complement(3667833..3670193) 1 NC_008702.1 General secretion pathway protein E,; Specificity unclear; general secretion pathway protein E 3670193 4609758 azo3328 Azoarcus sp. BH72 general secretion pathway protein E YP_934830.1 3667833 R 62928 CDS YP_934831.1 119899618 4607919 complement(3670262..3670960) 1 NC_008702.1 Probable 35-cyclic-nucleotide phosphodiesterase precursor (EC 3.1.4.17) (PDEase) (3:5-CNP). TREMBL:Q8ZD92:28% identity, 47% similarity InterPro; IPR001279; Blactmase-like. IPR000396; Pdiesterase2. Pfam: PF00753; Lactamase_B No transmembrane helices TIGR00010: deoxyribonuclease TatD fami; Function unclear; putative 3',5'-cyclic-nucleotide phosphodiesterase 3670960 4607919 azo3329 Azoarcus sp. BH72 putative 3',5'-cyclic-nucleotide phosphodiesterase YP_934831.1 3670262 R 62928 CDS YP_934832.1 119899619 4607920 complement(3671156..3671920) 1 NC_008702.1 Conserved hypothetical protein which may be involved into signal transduction processes. Important Domain: Pfam:PF00498 FHA domain. The forkhead-associated (FHA) domain is a phosphopeptide recognition domain found in many regulatory proteins. It displays specificity for phosphothreonine-containing epitopes but will also recognise phosphotyrosine with relatively high affinity. The domain is present in a diverse range of proteins, such as kinases, phosphatases, kinesins, transcription factors,RNA-binding proteins and metabolic enzymes.; FHA-domain-containing protein 3671920 4607920 azo3330 Azoarcus sp. BH72 FHA-domain-containing protein YP_934832.1 3671156 R 62928 CDS YP_934833.1 119899620 4607921 complement(3671969..3672724) 1 NC_008702.1 Putative phosphoprotein phosphatase,; Family membership; putative phosphoprotein phosphatase 3672724 pppL 4607921 pppL Azoarcus sp. BH72 putative phosphoprotein phosphatase YP_934833.1 3671969 R 62928 CDS YP_934834.1 119899621 4609614 complement(3672838..3673731) 1 NC_008702.1 Catalyzes the only substrate-level phosphorylation in the TCA cycle; succinyl-CoA synthetase subunit alpha 3673731 sucD 4609614 sucD Azoarcus sp. BH72 succinyl-CoA synthetase subunit alpha YP_934834.1 3672838 R 62928 CDS YP_934835.1 119899622 4609905 complement(3673743..3674903) 1 NC_008702.1 catalyzes the interconversion of succinyl-CoA and succinate; succinyl-CoA synthetase subunit beta 3674903 sucC 4609905 sucC Azoarcus sp. BH72 succinyl-CoA synthetase subunit beta YP_934835.1 3673743 R 62928 CDS YP_934836.1 119899623 4609904 complement(3675151..3675705) 1 NC_008702.1 Conserved hypothetical protein. Homology to BP2542 of B.pertussis of 50% (trembl:Q7VVU4). No domains predicted. No signal peptide. No TMHs; hypothetical protein 3675705 4609904 azo3334 Azoarcus sp. BH72 hypothetical protein YP_934836.1 3675151 R 62928 CDS YP_934837.1 119899624 4607922 3675922..3677832 1 NC_008702.1 In Pseudomonas aeruginosa, FimV is probable involved in remodelling of the peptidoglycan layer to enable assembly of the type IV fimbrial structure and machinery. And it is also required for twitching motility. Similar to trembl|O87015 (26%). Pfam (PF05489): Phage Tail Protein X SignalP reporting Signal peptide; Function unclear; putative type 4 pilus biogenesis 3677832 fimV2 4607922 fimV2 Azoarcus sp. BH72 putative type 4 pilus biogenesis YP_934837.1 3675922 D 62928 CDS YP_934838.1 119899625 4608765 complement(3677837..3678592) 1 NC_008702.1 Conserved hypothetical protein,ybgI, 48% identity(67% similarity) to SwissProt;P75743(E.coli)SP|Q8XFW7. SwissProt;Q8XVA0(58% identity)Ralstonia solanacearum. TrEMBL;Q82UH6(53% identity)Nitrosomonas europaea. Has PF01784, NIF3 (NGG1p interacting factor 3);IPR002678, DUF34; This family contains several NIF3 (NGG1p interacting factor 3) protein homologues. NIF3 interacts with the yeast transcriptional coactivator NGG1p which is part of the ADA complex, the exact function of this interaction is unknown.; hypothetical protein 3678592 4608765 azo3336 Azoarcus sp. BH72 hypothetical protein YP_934838.1 3677837 R 62928 CDS YP_934839.1 119899626 4607923 3678696..3679862 1 NC_008702.1 Probable serine protease AlgW. Homology to algW of P. aeruginosa of 48% (trembl|Q51374). InterPro: Serine proteases trypsin family (IPR001254); PDZ domain (Also known as DHR or GLGF) (IPR001478); HtrA/DegQ protease family (IPR001940) Pfam: PDZ domain (Also known as DHR or GLGF); Trypsin no signal peptide 1 TMH; High confidence in function and specificity; serine protease AlgW 3679862 algW 4607923 algW Azoarcus sp. BH72 serine protease AlgW YP_934839.1 3678696 D 62928 CDS YP_934840.1 119899627 4608300 complement(3679908..3680675) 1 NC_008702.1 Sec-independent protein translocase tatC. Required for correct localization of precursor proteins bearing signal peptides with the twin arginine conserved motif S/T-R-R-X-F-L-K. This sec-independent pathway is termed TAT for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. Sequence analysis predicts that TatC contains six transmembrane helices (TMHs), and experimental data confirmed that N and C termini of TatC or cpTatC are exposed to the cytoplasmic 2a6401s02: Sec-independent periplasmic Pfam: MttB family UPF0032 propable 6 TMHs, no signal peptide; High confidence in function and specificity; Sec-independent protein translocase 3680675 tatC 4608300 tatC Azoarcus sp. BH72 Sec-independent protein translocase YP_934840.1 3679908 R 62928 CDS YP_934841.1 119899628 4609920 complement(3680681..3681109) 1 NC_008702.1 Sec-independent protein translocase tatB. Required for correct localization of precursor proteins bearing signal peptides with the twin arginine conserved motif S/T-R-R-X-F-L-K. This sec-independent pathway is termed TAT for twin-arginine translocation system. This system mainly transports proteins with bound cofactors that require folding prior to export. no signal peptide probable TMHs: 1 TatB is essential for the secretion of these specific proteins, and forms a scaffold upon which TatC assembles in the membrane. Deletion studies have shown that it is crucial in the forming of the TAT complex, and is therefore necessary for the correct working of the system.; High confidence in function and specificity; putative Sec-independent protein translocase 3681109 tatB 4609920 tatB Azoarcus sp. BH72 putative Sec-independent protein translocase YP_934841.1 3680681 R 62928 CDS YP_934842.1 119899629 4609919 complement(3681177..3681410) 1 NC_008702.1 TatA; similar to TatE that is found in some proteobacteria; part of system that translocates proteins with a conserved twin arginine motif across the inner membrane; capable of translocating folded substrates typically those with bound cofactors; similar to a protein import system in thylakoid membranes; twin arginine translocase A 3681410 tatA 4609919 tatA Azoarcus sp. BH72 twin arginine translocase A YP_934842.1 3681177 R 62928 CDS YP_934843.1 119899630 4609918 3681361..3681534 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 3681534 4609918 azo3341 Azoarcus sp. BH72 hypothetical protein YP_934843.1 3681361 D 62928 CDS YP_934844.1 119899631 4607924 complement(3681597..3681920) 1 NC_008702.1 catalyzes the formation of 1-(5-phosphoribosyl)-AMP from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis; phosphoribosyl-ATP pyrophosphatase 3681920 hisE 4607924 hisE Azoarcus sp. BH72 phosphoribosyl-ATP pyrophosphatase YP_934844.1 3681597 R 62928 CDS YP_934845.1 119899632 4609004 complement(3681917..3682312) 1 NC_008702.1 PR-AMP cyclohydrolase; functions in histidine biosynthesis from PRPP; converts 1-(5-phosphoribosyl)-AMP to 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxyamide during the histidine biosynthesis pathway; binds zinc and magnesium; forms homodimers; phosphoribosyl-AMP cyclohydrolase 3682312 hisI 4609004 hisI Azoarcus sp. BH72 phosphoribosyl-AMP cyclohydrolase YP_934845.1 3681917 R 62928 CDS YP_934846.1 119899633 4609008 complement(3682309..3683067) 1 NC_008702.1 catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase; imidazole glycerol phosphate synthase subunit HisF 3683067 hisF 4609008 hisF Azoarcus sp. BH72 imidazole glycerol phosphate synthase subunit HisF YP_934846.1 3682309 R 62928 CDS YP_934847.1 119899634 4609005 complement(3683067..3683822) 1 NC_008702.1 catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-phosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide; 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase 3683822 hisA 4609005 hisA Azoarcus sp. BH72 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase YP_934847.1 3683067 R 62928 CDS YP_934848.1 119899635 4608999 complement(3683868..3684506) 1 NC_008702.1 with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide; imidazole glycerol phosphate synthase subunit HisH 3684506 hisH 4608999 hisH Azoarcus sp. BH72 imidazole glycerol phosphate synthase subunit HisH YP_934848.1 3683868 R 62928 CDS YP_934849.1 119899636 4609007 complement(3684561..3685190) 1 NC_008702.1 catalyzes the dehydration of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to 3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine biosynthesis; imidazoleglycerol-phosphate dehydratase 3685190 hisB 4609007 hisB Azoarcus sp. BH72 imidazoleglycerol-phosphate dehydratase YP_934849.1 3684561 R 62928 CDS YP_934850.1 119899637 4609000 3685259..3686347 1 NC_008702.1 catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis; histidinol-phosphate aminotransferase 3686347 hisC2 4609000 hisC2 Azoarcus sp. BH72 histidinol-phosphate aminotransferase YP_934850.1 3685259 D 62928 CDS YP_934851.1 119899638 4609002 complement(3686429..3687163) 1 NC_008702.1 Similar to phage protein P7. InterPro: SLT domain; Family membership; putative transglycosylase 3687163 4609002 azo3349 Azoarcus sp. BH72 putative transglycosylase YP_934851.1 3686429 R 62928 CDS YP_934852.1 119899639 4607925 complement(3687461..3688036) 1 NC_008702.1 Putative signaling protein in beta-lactamase regulation. AMPD seems not to act as a direct sensor for beta-lactams. It hydrolyzes 16-anhydro-N-acetylmuramoyl-L-alanyl-D-3glutamyl-meso- diaminopimelic acid to the tripeptide L-alanyl-gamma-D-glutamyl- meso-diaminopimelate (By similarity), TREMBL:Q8KRJ4 (63% identity); SWISSPROT:P82974 (60% identity). Pfam (PF01510): N-acetylmuramoyl-L-alanine amidase.; High confidence in function and specificity; N-acetyl-anhydromuranmyl-L-alanine amidase 3688036 ampD2 4607925 ampD2 Azoarcus sp. BH72 N-acetyl-anhydromuranmyl-L-alanine amidase YP_934852.1 3687461 R 62928 CDS YP_934853.1 119899640 4608313 complement(3688047..3688952) 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs, TREMBL:O59091 (37% identity); TREMBL:Q7NDM5 (40% identity). Pfam (PF00005): ABC transporter. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; High confidence in function and specificity; putative ABC transporter ATP-binding protein 3688952 4608313 azo3351 Azoarcus sp. BH72 putative ABC transporter ATP-binding protein YP_934853.1 3688047 R 62928 CDS YP_934854.1 119899641 4607926 complement(3688949..3689800) 1 NC_008702.1 Similar to hypothetical protein GSU1500 of Geobacter sulfurreducens PCA (16%). TMHMM2 reporting one TMhelix. Sigcleave reporting one SignalPeptide. Coils2 reporting coiled coil region; hypothetical protein 3689800 4607926 azo3352 Azoarcus sp. BH72 hypothetical protein YP_934854.1 3688949 R 62928 CDS YP_934855.1 119899642 4607927 complement(3689807..3690616) 1 NC_008702.1 In Myxococcus xanthus pilI are co-transcribed with pilH an ABC transporter homologue required for type IV pilus biogenesis and social gliding. pilI appear to be functionally related to pilH. Similar to membrane protein of Geobacter sulfurreducens PCA (14%) and weak; Conserved hypothetical protein; hypothetical protein 3690616 4607927 azo3353 Azoarcus sp. BH72 hypothetical protein YP_934855.1 3689807 R 62928 CDS YP_934856.1 119899643 4607928 complement(3690780..3691208) 1 NC_008702.1 PilB encodes for a 14.2 kDa polypeptide showing similarity to FimF, a component of type I fimbriae of E. coli. It may be part of the pilus assembly complex or the pilus itself. (Reference: Doerr et al., 1998 Molecular Microbiology); Specificity unclear; Type IV pilus assembly protein 3691208 pilB 4607928 pilB Azoarcus sp. BH72 Type IV pilus assembly protein YP_934856.1 3690780 R 62928 CDS YP_934857.1 119899644 4609555 complement(3691277..3691456) 1 NC_008702.1 PilA encodes an unusually short (6.4 kDa)putative pilin precursor showing 100% sequence identity to the conserved N-terminus of the Pseudomonas aeruginosa type IV pilin. (Reference: Doerr et al., 1998 Molecular Microbiology); High confidence in function and specificity; tfp structural protein 3691456 pilA 4609555 pilA Azoarcus sp. BH72 tfp structural protein YP_934857.1 3691277 R 62928 CDS YP_934858.1 119899645 4609554 complement(3691660..3693036) 1 NC_008702.1 Two component system response regulator,; High confidence in function and specificity; two component system response regulator 3693036 pilR 4609554 pilR Azoarcus sp. BH72 two component system response regulator YP_934858.1 3691660 R 62928 CDS YP_934859.1 119899646 4609571 complement(3693033..3694586) 1 NC_008702.1 Two component system sensor protein,; High confidence in function and specificity; two component system sensor protein 3694586 pilS 4609571 pilS Azoarcus sp. BH72 two component system sensor protein YP_934859.1 3693033 R 62928 CDS YP_934860.1 119899647 4609572 complement(3694588..3694929) 1 NC_008702.1 Hypothetical membrane protein. No real homology of the entire protein to the data bank. no domains predicted no signal peptide 1TMH; hypothetical protein 3694929 4609572 azo3358 Azoarcus sp. BH72 hypothetical protein YP_934860.1 3694588 R 62928 CDS YP_934861.1 119899648 4607929 complement(3694971..3696188) 1 NC_008702.1 catalyzes the transfer of an amino moiety; putative aminotransferase 3696188 ybdL 4607929 ybdL Azoarcus sp. BH72 putative aminotransferase YP_934861.1 3694971 R 62928 CDS YP_934862.1 119899649 4610146 3696278..3697354 1 NC_008702.1 isomerizes methylthioribose-1-phosphate into methylthioribulose-1-phosphate; involved in methionine salvage pathway; methylthioribose-1-phosphate isomerase 3697354 mtnA 4610146 mtnA Azoarcus sp. BH72 methylthioribose-1-phosphate isomerase YP_934862.1 3696278 D 62928 CDS YP_934863.1 119899650 4608664 3697351..3698049 1 NC_008702.1 L-fuculose phosphate aldolase(L-fuculose-1-phosphate aldolase). Involved in the fucose metabolism catalyzing the third step of the pathway, L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde. 41% Aldolase_II_N.IPR004782; FucA. Pfam:PF00596; Aldolase_II; 1. TIGRFAMs:TIGR01086; fucA; 1.; High confidence in function and specificity; putative L-fuculose phosphate aldolase 3698049 fucA 4608664 fucA Azoarcus sp. BH72 putative L-fuculose phosphate aldolase YP_934863.1 3697351 D 62928 CDS YP_934864.1 119899651 4608844 complement(3698348..3699238) 1 NC_008702.1 Putative 2-hydroxy-3-oxopropionate reductase Homology to glxR of E. coli of 39% (SWISSPROT:GLXR_ECOLI) Activity:- (R)-glycerate + NAD(P)(+) = 2-hydroxy-3-oxopropanoate + NAD(P)H. InterPro: 3-hydroyisobutyrate dehydrogenase (IPR002204) Pfam: Nad binding domain of 6-phosphoglucanate dehydrogenase (PF03446) Tigrfam: gnd_rel: 6-phosphoglucante no signal peptide no TMHs; Family membership; putative 2-hydroxy-3-oxopropionate reductase 3699238 glxR1 4608844 glxR1 Azoarcus sp. BH72 putative 2-hydroxy-3-oxopropionate reductase YP_934864.1 3698348 R 62928 CDS YP_934865.1 119899652 4608922 complement(3699235..3700116) 1 NC_008702.1 Probable 2-hydroxy-3-oxopropionate reductase Homology to glxR of E. coli of 46% (SWISSPROT:GLXR_ECOLI) Activity:- (R)-glycerate + NAD(P)(+) = 2-hydroxy-3-oxopropanoate + NAD(P)H. InterPro: 3-hydroyisobutyrate dehydrogenase (IPR002204) Pfam: Nad binding domain of 6-phosphoglucanate dehydrogenase (PF03446) Tigrfam: gnd_rel: 6-phosphoglucante no signal peptide no TMHs 0; High confidence in function and specificity; 2-hydroxy-3-oxopropionate reductase 3700116 glxR2 4608922 glxR2 Azoarcus sp. BH72 2-hydroxy-3-oxopropionate reductase YP_934865.1 3699235 R 62928 CDS YP_934866.1 119899653 4608923 3700206..3701126 1 NC_008702.1 The glycosyl transferase family includes anthranilate phosphoribosyltransferase (TrpD, EC: 2.4.2.18) and thymidine phosphorylase (EC: 2.4.2.2). All these proteins can transfer a phosphorylated ribose substrate. Similar to sprot|YBIB_ECOLI (40%) and to sprot|TRPD_SULSO (25%). Pfam (PF02885): Glycosyl transferase, family 3; Family membership; glycosyl transferase family protein 3701126 4608923 azo3364 Azoarcus sp. BH72 glycosyl transferase family protein YP_934866.1 3700206 D 62928 CDS YP_934867.1 119899654 4607930 complement(3701590..3702435) 1 NC_008702.1 Putative pyrophosphorylase modD (EC 2.4.2.-). InterPro: Quinolinate phosphoribosyl transferase; Specificity unclear; putative molybdenum transport protein 3702435 modD 4607930 modD Azoarcus sp. BH72 putative molybdenum transport protein YP_934867.1 3701590 R 62928 CDS YP_934868.1 119899655 4609254 complement(3702524..3702907) 1 NC_008702.1 Conserved hypothetical protein. Homology to rpa3088 of R. palustris of 65% (tremblnew|CAE28529). Pfam: Dinitrogenase iron-molybdenum cofactor. no signal peptide. no TMHs.; hypothetical protein 3702907 4609254 azo3366 Azoarcus sp. BH72 hypothetical protein YP_934868.1 3702524 R 62928 CDS YP_934869.1 119899656 4607931 complement(3702913..3703188) 1 NC_008702.1 Putative NifZ Protein homolog, shares 45% similarity to SwissProt:P46040, NifZ protein entry. Has PF04319:IPR007415:NifZ domain;This short protein is found in the nif (nitrogen fixation) operon. Its function is unknown but is probably involved in nitrogen fixation or regulating some component of this process. This 75 residue region is presumed to be a domain. It is found in isolation in some members and in the amino terminal half of the longer NifZ proteins. No Signal peptide present. No TMH Present.; High confidence in function and specificity; putative NifZ protein 3703188 4607931 azo3367 Azoarcus sp. BH72 putative NifZ protein YP_934869.1 3702913 R 62928 CDS YP_934870.1 119899657 4607932 complement(3703191..3703517) 1 NC_008702.1 Probable ferredoxin 2fe-2s protein. Homology to fer2 of C. pasteruianum of 43% (sprot|FER2_CLOPA). Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. no signal peptide no TMHs; Family membership; ferredoxin 2Fe-2S protein 3703517 fer22 4607932 fer22 Azoarcus sp. BH72 ferredoxin 2Fe-2S protein YP_934870.1 3703191 R 62928 CDS YP_934871.1 119899658 4608758 complement(3703673..3704557) 1 NC_008702.1 Pirin-like protein. TREMBL:8Y1Z6: 68% identity, 80% similarity InterPro: DUF209 InterPro:IPR008778; Pirin_C. IPR003829; Pirin_N. Pfam :PF02678; Pirin; 1. PF05726; Pirin_C TIGR00292: thiazole biosynthesis enzyme No signal peptide (Signal P predicted). No transmembrane helices (TMHMM predicted).; Specificity unclear; hypothetical protein 3704557 4608758 azo3369 Azoarcus sp. BH72 hypothetical protein YP_934871.1 3703673 R 62928 CDS YP_934872.1 119899659 4607933 complement(3704618..3705220) 1 NC_008702.1 Converts holo-ACP to apo-ACP by hydrolytic cleavage of the phosphopantetheine prosthetic group from ACP (By similarity). Entry name :- SWISSPROT:ACPD_ECOLI InterPro:-IPR003680; NADHdh_2. Pfam:- PF02525; Flavodoxin_2; 1. Identities = 97/180 (53%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; acyl carrier protein phosphodiesterase 3705220 acpD 4607933 acpD Azoarcus sp. BH72 acyl carrier protein phosphodiesterase YP_934872.1 3704618 R 62928 CDS YP_934873.1 119899660 4608269 3705329..3706252 1 NC_008702.1 Putative transcriptional regulator, LysR family,; High confidence in function and specificity; LysR family transcriptional regulator 3706252 ltrA 4608269 ltrA Azoarcus sp. BH72 LysR family transcriptional regulator YP_934873.1 3705329 D 62928 CDS YP_934874.1 119899661 4609183 3706379..3706879 1 NC_008702.1 Disulfide bond formation protein B. Homology to dsdB of B. cepacia of 38% (BAA11858). Required for disulfide bond formation in some periplasmic proteins. Acts by oxidizing the dsbA protein (By similarity). InterPro: Disulfide bond formation protein DsbB (IPR3752). Pfam: Disulfide bond formation protein DsbB signal peptide probable 3 to 4 TMHs; Family membership; putative disulfide bond formation protein B 3706879 dsbB 4609183 dsbB Azoarcus sp. BH72 putative disulfide bond formation protein B YP_934874.1 3706379 D 62928 CDS YP_934875.1 119899662 4608654 complement(3706887..3707195) 1 NC_008702.1 Conserved hypothetical protein. Homology to cv1788 of C. violaceum of 33% (trembl|Q7NX41). no domains predicted. no signal peptide. no TMHS.; hypothetical protein 3707195 4608654 azo3373 Azoarcus sp. BH72 hypothetical protein YP_934875.1 3706887 R 62928 CDS YP_934876.1 119899663 4607934 complement(3707307..3707525) 1 NC_008702.1 Homolog to the hypothetical protein ygaP from E.coli. SWISSPROT:YGAP_ECOLI InterPro: IPR001763; Rhodanese-like. Pfam: PF00581; Rhodanese; 1. TMhelix:2 present.; Function unclear; thiosulfate sulfurtransferase 3707525 4607934 azo3374 Azoarcus sp. BH72 thiosulfate sulfurtransferase YP_934876.1 3707307 R 62928 CDS YP_934877.1 119899664 4607935 complement(3707522..3710800) 1 NC_008702.1 AcrB/AcrD/AcrF family. Members of this family are integral membrane proteins. Some are involved in drug resistance. AcrB cooperates with a membrane fusion protein, AcrA, and an outer membrane channel TolC. The structure shows the AcrB forms a homotrimer, TREMBL:Q8KAV4 (43% identity); SWISSPROT:P25197 (23% identity). InterPro (IPR001036): Acriflavin resistance protein. PFam (PF00873): AcrB/AcrD/AcrF family. TIGRFAM (TIGR00914): Heavy metal efflux pump, CzcA family. TIGRFAM (TIGR00915): Hydrophobe/Amphiphile Efflux-1 (HAE1) Family protein. TMHMM predicting 11 transmembrane helices. TC (2.A.6.2): The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family.; Specificity unclear; RND efflux transporter permease 3710800 4607935 azo3375 Azoarcus sp. BH72 RND efflux transporter permease YP_934877.1 3707522 R 62928 CDS YP_934878.1 119899665 4607936 complement(3710800..3711864) 1 NC_008702.1 HlyD family secretion protein. The secretion of a number of proteins/molecules require the help of members belonging to the ABC transporter family and a membrane fusion protein belonging to the HlyD family. SignalP predicting signal peptide. TC (2.A.6.1): The Heavy Metal Efflux (HME) Family.; Family membership; membrane fusion protein 3711864 4607936 azo3376 Azoarcus sp. BH72 membrane fusion protein YP_934878.1 3710800 R 62928 CDS YP_934879.1 119899666 4607937 complement(3711911..3712303) 1 NC_008702.1 Conserved hypothetical protein. Homology to Bcep18194_A5800 of Burkholderia sp. 383 of 53%. Pfam: CbiX. The function of CbiX is uncertain, however it is found in cobalamin biosynthesis operons and so may have a related function. Some CbiX proteins contain a striking histidine-rich region at their C-terminus, which suggests that it might be involved in metal chelation. No signal peptide. No TMHs.; hypothetical protein 3712303 4607937 azo3377 Azoarcus sp. BH72 hypothetical protein YP_934879.1 3711911 R 62928 CDS YP_934880.1 119899667 4607938 complement(3712312..3713148) 1 NC_008702.1 Region start changed from 3713308 to 3713122 (-186 bases); HesA/MoeB/ThiF family protein 3713148 4607938 azo3378 Azoarcus sp. BH72 HesA/MoeB/ThiF family protein YP_934880.1 3712312 R 62928 CDS YP_934881.1 119899668 4607939 complement(3713397..3714398) 1 NC_008702.1 Probable metallo betalactamase related protein with 47% identity and 65% similarity (TREMBL:Q82SI4). Pfam: Chitin synthetase, SOR/SNZ family, Thiolase N-terminal domain, phosphotyrosine interaction domain. TMHMM predicted transmembrane helix.; Function unclear; hydroxyacylglutathione hydrolase 3714398 4607939 azo3379 Azoarcus sp. BH72 hydroxyacylglutathione hydrolase YP_934881.1 3713397 R 62928 CDS YP_934882.1 119899669 4607940 3714479..3715621 1 NC_008702.1 Region start changed from 3714581 to 3714479 (102 bases); putative transaminase 3715621 4607940 azo3380 Azoarcus sp. BH72 putative transaminase YP_934882.1 3714479 D 62928 CDS YP_934883.1 119899670 4607941 complement(3715626..3718484) 1 NC_008702.1 Hypothetical signaling protein; signaling protein 3718484 4607941 azo3381 Azoarcus sp. BH72 signaling protein YP_934883.1 3715626 R 62928 CDS YP_934884.1 119899671 4607942 complement(3718505..3718960) 1 NC_008702.1 Putative two-component system regulatory protein,weak similarity to SWISSPROT: sprot|RCP1_SYNY3 (32% Synechocystis sp., response regulator Rcp1 ) InterPro: IPR001789 Response_reg. Pfam: PF00072 response_reg. SMART: SM00448 REC. Response regulator rcp1. FORMS A TWO-COMPONENT SYSTEM WITH CPH1 IN WHICH IT ACTS AS RECEIVER SUBSTRATE.; Family membership; putative two-component system regulatory protein 3718960 rcp3 4607942 rcp3 Azoarcus sp. BH72 putative two-component system regulatory protein YP_934884.1 3718505 R 62928 CDS YP_934885.1 119899672 4609698 complement(3719043..3721649) 1 NC_008702.1 Putative two-component system sensor protein, weak similarity to SWISSPROT: sprot|PHY1_SYNY3 (10% Synechocystis sp., Cph1) InterPro: IPR003594 ATPbind_ATPase. IPR004358 Bact_sens_pr_C. IPR003661 His_kinA_N. IPR005467 His_kinase. IPR003660 HAMP. IPR000014 PAS_domain. IPR001610 PAC motif. Pfam: PF00672 HAMP. PF02518 HATPase_c. PF00512 HisKA. PF00989 PAS domain. PF00785 PAC motif SMART: SM00304 HAMP. SM00387 HATPase_c. SM00388 HisKA. SM00091 PAS. SM00086 PAC. TIGRFAM: TIGR00229 PAS domain S-box. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices. Phytochrome-like protein cph1 (EC 2.7.3.-) (Light-regulated histidine kinase 1) (Bacteriophytochrome cph1). REGULATORY PHOTORECEPTOR WHICH EXISTS IN TWO FORMS THAT ARE REVERSIBLY INTERCONVERTIBLE BY LIGHT: THE R FORM THAT ABSORBS MAXIMALLY IN THE RED REGION OF THE SPECTRUM AND THE FR FORM THAT ABSORBS MAXIMALLY IN THE FAR-RED REGION. HAS ALSO A SLIGHT BLUE SHIFT FOR THE FAR-RED MAXIMUM. FORMS A TWO-COMPONENT SYSTEM WITH THE RCP1 RESPONSE REGULATOR.; Conserved hypothetical protein; putative two-component system sensor protein 3721649 cph2 4609698 cph2 Azoarcus sp. BH72 putative two-component system sensor protein YP_934885.1 3719043 R 62928 CDS YP_934886.1 119899673 4608527 complement(3721646..3722614) 1 NC_008702.1 Putative aliphatic sulfonates binding protein precursor.PART OF A BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEM FOR ALIPHATIC SULFONATES. PUTATIVE BINDING PROTEIN. 22% SBP_bac_3. SMART: SM00062; PBPb; 1.; Specificity unclear; putative aliphatic sulfonates binding protein 3722614 4608527 azo3384 Azoarcus sp. BH72 putative aliphatic sulfonates binding protein YP_934886.1 3721646 R 62928 CDS YP_934887.1 119899674 4607943 complement(3722791..3723273) 1 NC_008702.1 Single-strand binding protein (Helix-destabilizing protein). This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).; High confidence in function and specificity; single-strand DNA-binding protein 3723273 ssb 4607943 ssb Azoarcus sp. BH72 single-strand DNA-binding protein YP_934887.1 3722791 R 62928 CDS YP_934888.1 119899675 4609892 complement(3723325..3724530) 1 NC_008702.1 Hypothetical transport protein yajR. InterPro: General substrate transporters 2_A_01_02: Multidrug resistance protein; Specificity unclear; putative transport protein 3724530 4609892 azo3386 Azoarcus sp. BH72 putative transport protein YP_934888.1 3723325 R 62928 CDS YP_934889.1 119899676 4607944 3724646..3727483 1 NC_008702.1 UvrABC system protein A (UvrA protein) . The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB the uvrA molecules dissociate (By similarity). InterPro: Excinuclease ABC A subunit uvra: excinuclease ABC A subunit.; High confidence in function and specificity; excinuclease ABC subunit A 3727483 uvrA2 4607944 uvrA2 Azoarcus sp. BH72 excinuclease ABC subunit A YP_934889.1 3724646 D 62928 CDS YP_934890.1 119899677 4610081 3727563..3727862 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 3727862 4610081 azo3388 Azoarcus sp. BH72 hypothetical protein YP_934890.1 3727563 D 62928 CDS YP_934891.1 119899678 4607945 complement(3727870..3728883) 1 NC_008702.1 UDP-glucose 4-epimerase (EC 5.1.3.2) (Galactowaldenase) (UDP- galactose 4-epimerase). InterPro: NAD dependent epimerase/dehydratase family; High confidence in function and specificity; UDP-glucose 4-epimerase 3728883 galE2 4607945 galE2 Azoarcus sp. BH72 UDP-glucose 4-epimerase YP_934891.1 3727870 R 62928 CDS YP_934892.1 119899679 4608856 complement(3728974..3729369) 1 NC_008702.1 is a component of the macrolide binding site in the peptidyl transferase center; 50S ribosomal protein L17 3729369 rplQ 4608856 rplQ Azoarcus sp. BH72 50S ribosomal protein L17 YP_934892.1 3728974 R 62928 CDS YP_934893.1 119899680 4609776 complement(3729396..3730376) 1 NC_008702.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 3730376 rpoA 4609776 rpoA Azoarcus sp. BH72 DNA-directed RNA polymerase subunit alpha YP_934893.1 3729396 R 62928 CDS YP_934894.1 119899681 4609794 complement(3730415..3731044) 1 NC_008702.1 primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination; 30S ribosomal protein S4 3731044 rpsD 4609794 rpsD Azoarcus sp. BH72 30S ribosomal protein S4 YP_934894.1 3730415 R 62928 CDS YP_934895.1 119899682 4609807 complement(3731059..3731448) 1 NC_008702.1 located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3; 30S ribosomal protein S11 3731448 rpsK 4609807 rpsK Azoarcus sp. BH72 30S ribosomal protein S11 YP_934895.1 3731059 R 62928 CDS YP_934896.1 119899683 4609814 complement(3731461..3731823) 1 NC_008702.1 located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA; 30S ribosomal protein S13 3731823 rpsM 4609814 rpsM Azoarcus sp. BH72 30S ribosomal protein S13 YP_934896.1 3731461 R 62928 CDS YP_934897.1 119899684 4609816 complement(3731877..3731990) 1 NC_008702.1 smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif; 50S ribosomal protein L36 3731990 rpmJ 4609816 rpmJ Azoarcus sp. BH72 50S ribosomal protein L36 YP_934897.1 3731877 R 62928 CDS YP_934898.1 119899685 4609793 complement(3732016..3732234) 1 NC_008702.1 stimulates the activities of the other two initiation factors, IF-2 and IF-3; translation initiation factor IF-1 3732234 infA 4609793 infA Azoarcus sp. BH72 translation initiation factor IF-1 YP_934898.1 3732016 R 62928 CDS YP_934899.1 119899686 4609082 complement(3732238..3733563) 1 NC_008702.1 forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase; preprotein translocase subunit SecY 3733563 secY 4609082 secY Azoarcus sp. BH72 preprotein translocase subunit SecY YP_934899.1 3732238 R 62928 CDS YP_934900.1 119899687 4609863 complement(3733578..3734012) 1 NC_008702.1 late assembly protein; 50S ribosomal protein L15 3734012 rplO 4609863 rplO Azoarcus sp. BH72 50S ribosomal protein L15 YP_934900.1 3733578 R 62928 CDS YP_934901.1 119899688 4609774 complement(3734014..3734196) 1 NC_008702.1 L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7; 50S ribosomal protein L30 3734196 rpmD 4609774 rpmD Azoarcus sp. BH72 50S ribosomal protein L30 YP_934901.1 3734014 R 62928 CDS YP_934902.1 119899689 4609788 complement(3734200..3734724) 1 NC_008702.1 located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance; 30S ribosomal protein S5 3734724 rpsE 4609788 rpsE Azoarcus sp. BH72 30S ribosomal protein S5 YP_934902.1 3734200 R 62928 CDS YP_934903.1 119899690 4609808 complement(3734737..3735090) 1 NC_008702.1 binds 5S rRNA along with protein L5 and L25; 50S ribosomal protein L18 3735090 rplR 4609808 rplR Azoarcus sp. BH72 50S ribosomal protein L18 YP_934903.1 3734737 R 62928 CDS YP_934904.1 119899691 4609777 complement(3735102..3735635) 1 NC_008702.1 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance; 50S ribosomal protein L6 3735635 rplF 4609777 rplF Azoarcus sp. BH72 50S ribosomal protein L6 YP_934904.1 3735102 R 62928 CDS YP_934905.1 119899692 4609767 complement(3735645..3736040) 1 NC_008702.1 binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; 30S ribosomal protein S8 3736040 rpsH 4609767 rpsH Azoarcus sp. BH72 30S ribosomal protein S8 YP_934905.1 3735645 R 62928 CDS YP_934906.1 119899693 4609811 complement(3736054..3736359) 1 NC_008702.1 located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif; 30S ribosomal protein S14 3736359 rpsN 4609811 rpsN Azoarcus sp. BH72 30S ribosomal protein S14 YP_934906.1 3736054 R 62928 CDS YP_934907.1 119899694 4609817 complement(3736367..3736906) 1 NC_008702.1 part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13; 50S ribosomal protein L5 3736906 rplE 4609817 rplE Azoarcus sp. BH72 50S ribosomal protein L5 YP_934907.1 3736367 R 62928 CDS YP_934908.1 119899695 4609766 complement(3736916..3737233) 1 NC_008702.1 assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel; 50S ribosomal protein L24 3737233 rplX 4609766 rplX Azoarcus sp. BH72 50S ribosomal protein L24 YP_934908.1 3736916 R 62928 CDS YP_934909.1 119899696 4609783 complement(3737246..3737614) 1 NC_008702.1 50S ribosomal protein L14.; High confidence in function and specificity; 50S ribosomal protein L14 3737614 rplN 4609783 rplN Azoarcus sp. BH72 50S ribosomal protein L14 YP_934909.1 3737246 R 62928 CDS YP_934910.1 119899697 4609773 complement(3737766..3738035) 1 NC_008702.1 primary binding protein; helps mediate assembly; involved in translation fidelity; 30S ribosomal protein S17 3738035 rpsQ 4609773 rpsQ Azoarcus sp. BH72 30S ribosomal protein S17 YP_934910.1 3737766 R 62928 CDS YP_934911.1 119899698 4609820 complement(3738032..3738226) 1 NC_008702.1 one of the stabilizing components for the large ribosomal subunit; 50S ribosomal protein L29 3738226 rpmC 4609820 rpmC Azoarcus sp. BH72 50S ribosomal protein L29 YP_934911.1 3738032 R 62928 CDS YP_934912.1 119899699 4609787 complement(3738229..3738642) 1 NC_008702.1 located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e; 50S ribosomal protein L16 3738642 rplP 4609787 rplP Azoarcus sp. BH72 50S ribosomal protein L16 YP_934912.1 3738229 R 62928 CDS YP_934913.1 119899700 4609775 complement(3738642..3739424) 1 NC_008702.1 forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation; 30S ribosomal protein S3 3739424 rpsC 4609775 rpsC Azoarcus sp. BH72 30S ribosomal protein S3 YP_934913.1 3738642 R 62928 CDS YP_934914.1 119899701 4609806 complement(3739434..3739763) 1 NC_008702.1 binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center; 50S ribosomal protein L22 3739763 rplV 4609806 rplV Azoarcus sp. BH72 50S ribosomal protein L22 YP_934914.1 3739434 R 62928 CDS YP_934915.1 119899702 4609781 complement(3739773..3740048) 1 NC_008702.1 protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA; 30S ribosomal protein S19 3740048 rpsS 4609781 rpsS Azoarcus sp. BH72 30S ribosomal protein S19 YP_934915.1 3739773 R 62928 CDS YP_934916.1 119899703 4609822 complement(3740059..3740886) 1 NC_008702.1 one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; 50S ribosomal protein L2 3740886 rplB 4609822 rplB Azoarcus sp. BH72 50S ribosomal protein L2 YP_934916.1 3740059 R 62928 CDS YP_934917.1 119899704 4609763 complement(3740893..3741198) 1 NC_008702.1 binds third domain of 23S rRNA and protein L29; part of exit tunnel; 50S ribosomal protein L23 3741198 rplW 4609763 rplW Azoarcus sp. BH72 50S ribosomal protein L23 YP_934917.1 3740893 R 62928 CDS YP_934918.1 119899705 4609782 complement(3741195..3741815) 1 NC_008702.1 L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA; 50S ribosomal protein L4 3741815 rplD 4609782 rplD Azoarcus sp. BH72 50S ribosomal protein L4 YP_934918.1 3741195 R 62928 CDS YP_934919.1 119899706 4609765 complement(3741830..3742468) 1 NC_008702.1 binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin; 50S ribosomal protein L3 3742468 rplC 4609765 rplC Azoarcus sp. BH72 50S ribosomal protein L3 YP_934919.1 3741830 R 62928 CDS YP_934920.1 119899707 4609764 complement(3742583..3742894) 1 NC_008702.1 NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex; 30S ribosomal protein S10 3742894 rpsJ 4609764 rpsJ Azoarcus sp. BH72 30S ribosomal protein S10 YP_934920.1 3742583 R 62928 CDS YP_934921.1 119899708 4609813 complement(3742952..3744142) 1 NC_008702.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 3744142 tufA 4609813 tufA Azoarcus sp. BH72 elongation factor Tu YP_934921.1 3742952 R 62928 CDS YP_934922.1 119899709 4610048 complement(3744170..3746266) 1 NC_008702.1 EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 3746266 fusA2 4610048 fusA2 Azoarcus sp. BH72 elongation factor G YP_934922.1 3744170 R 62928 CDS YP_934923.1 119899710 4608849 complement(3746378..3746848) 1 NC_008702.1 binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit; 30S ribosomal protein S7 3746848 rpsG 4608849 rpsG Azoarcus sp. BH72 30S ribosomal protein S7 YP_934923.1 3746378 R 62928 CDS YP_934924.1 119899711 4609810 complement(3746882..3747259) 1 NC_008702.1 interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance; 30S ribosomal protein S12 3747259 rpsL 4609810 rpsL Azoarcus sp. BH72 30S ribosomal protein S12 YP_934924.1 3746882 R 62928 CDS YP_934925.1 119899712 4609815 complement(3747393..3751607) 1 NC_008702.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit beta' binds to sigma factor allowing it to bind to the -10 region of the promoter; DNA-directed RNA polymerase subunit beta' 3751607 rpoC 4609815 rpoC Azoarcus sp. BH72 DNA-directed RNA polymerase subunit beta' YP_934925.1 3747393 R 62928 CDS YP_934926.1 119899713 4609796 complement(3751682..3755815) 1 NC_008702.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme; DNA-directed RNA polymerase subunit beta 3755815 rpoB 4609796 rpoB Azoarcus sp. BH72 DNA-directed RNA polymerase subunit beta YP_934926.1 3751682 R 62928 CDS YP_934927.1 119899714 4609795 complement(3755976..3756353) 1 NC_008702.1 present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors; 50S ribosomal protein L7/L12 3756353 rplL 4609795 rplL Azoarcus sp. BH72 50S ribosomal protein L7/L12 YP_934927.1 3755976 R 62928 CDS YP_934928.1 119899715 4609771 complement(3756405..3756908) 1 NC_008702.1 binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit; 50S ribosomal protein L10 3756908 rplJ 4609771 rplJ Azoarcus sp. BH72 50S ribosomal protein L10 YP_934928.1 3756405 R 62928 CDS YP_934929.1 119899716 4609769 complement(3757142..3757837) 1 NC_008702.1 in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA; 50S ribosomal protein L1 3757837 rplA 4609769 rplA Azoarcus sp. BH72 50S ribosomal protein L1 YP_934929.1 3757142 R 62928 CDS YP_934930.1 119899717 4609762 complement(3757840..3758271) 1 NC_008702.1 binds directly to 23S ribosomal RNA; 50S ribosomal protein L11 3758271 rplK 4609762 rplK Azoarcus sp. BH72 50S ribosomal protein L11 YP_934930.1 3757840 R 62928 CDS YP_934931.1 119899718 4609770 complement(3758376..3758915) 1 NC_008702.1 Modulates Rho-dependent transcription termination; transcription antitermination protein NusG 3758915 nusG 4609770 nusG Azoarcus sp. BH72 transcription antitermination protein NusG YP_934931.1 3758376 R 62928 CDS YP_934932.1 119899719 4609435 complement(3758912..3759259) 1 NC_008702.1 forms a complex with SecY and SecG; SecYEG forms a putative protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force; preprotein translocase subunit SecE 3759259 secE 4609435 secE Azoarcus sp. BH72 preprotein translocase subunit SecE YP_934932.1 3758912 R 62928 CDS YP_934933.1 119899720 4610023 complement(3759422..3760612) 1 NC_008702.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 3760612 tufB 4610023 tufB Azoarcus sp. BH72 elongation factor Tu YP_934933.1 3759422 R 62928 CDS YP_934934.1 119899721 4610024 3761183..3762592 1 NC_008702.1 D-lactate dehydrogenase. Homology to dld of Mus musculus of 52% (trembl|Q8CIV4) CATALYZE THE STEREOSPECIFIC OXIDATION OF D-LACTATE TO PYRUVATE. InterPro: FAD linked oxidase C-terminal (IPR004113); FAD linked oxidase, N-terminal (IPR006093) Pfam: FAD binding damian; FAD linked oxidases, C-terminal domain Tigrfam: glcD: glycolate oxidase subunit GlcD no signal peptide no TMHs; Family membership; D-lactate dehydrogenase 3762592 dld 4610024 dld Azoarcus sp. BH72 D-lactate dehydrogenase YP_934934.1 3761183 D 62928 CDS YP_934935.1 119899722 4608626 3762769..3766365 1 NC_008702.1 Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates; indolepyruvate ferredoxin oxidoreductase 3766365 4608626 azo3433 Azoarcus sp. BH72 indolepyruvate ferredoxin oxidoreductase YP_934935.1 3762769 D 62928 CDS YP_934936.1 119899723 4607946 3766433..3767197 1 NC_008702.1 Conserved hypothetical membrane protein. Similar to TREMBL:Q82Y44 (38% identity); TREMBL:Q7NWG1 (37% identity). TMHMM reporting four transmembrane helices. signal peptide; Conserved hypothetical protein; hypothetical protein 3767197 4607946 azo3434 Azoarcus sp. BH72 hypothetical protein YP_934936.1 3766433 D 62928 CDS YP_934937.1 119899724 4607947 3767294..3767998 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology of the entire protein to the data bank Pfam: CobQ/CobB/MinD/ParA nucleotide binding domain no signal peptide no TMHs; hypothetical protein 3767998 4607947 azo3435 Azoarcus sp. BH72 hypothetical protein YP_934937.1 3767294 D 62928 CDS YP_934938.1 119899725 4607948 complement(3768050..3768676) 1 NC_008702.1 Probable DNA-binding response regulator, LuxR family,; Specificity unclear; LuxR family DNA-binding response regulator 3768676 flcA 4607948 flcA Azoarcus sp. BH72 LuxR family DNA-binding response regulator YP_934938.1 3768050 R 62928 CDS YP_934939.1 119899726 4608770 3768936..3769865 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA3798 of Azoarcus sp. EbN1 of 42% (gnl|keqq|eba:ebA3798(KEGG)). Pfam: DUF534. This is a family of putative secreted proteins of unknown function. signal peptide. no TMHs.; Conserved hypothetical protein; hypothetical protein 3769865 4608770 azo3437 Azoarcus sp. BH72 hypothetical protein YP_934939.1 3768936 D 62928 CDS YP_934940.1 119899727 4607949 3769862..3771781 1 NC_008702.1 Putative virulence regulator protein,ATPbind_ATPase. IPR005467; His_kinase. IPR003661; His_kinA_N. IPR003660; HAMP. IPR001789; Response_reg. Pfam: PF02518; HATPase_c. PF00512; HisKA. PF00672; HAMP. PF00072; Response_reg. SMART: SM00387; HATPase_c. SM00388; HisKA. SM00304; HAMP. SM00448; REC. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Specificity unclear; putative virulence regulator protein 3771781 vsrB 4607949 vsrB Azoarcus sp. BH72 putative virulence regulator protein YP_934940.1 3769862 D 62928 CDS YP_934941.1 119899728 4610097 3771856..3772257 1 NC_008702.1 conserved hypothetical protein. Homology to ebB122 of Azoarcus sp. EbN1 of 65% (gnl|keqq|eba:ebB122(KEGG)). No domains predicted. NO signal peptide present. No TMH present.; hypothetical protein 3772257 4610097 azo3439 Azoarcus sp. BH72 hypothetical protein YP_934941.1 3771856 D 62928 CDS YP_934942.1 119899729 4607950 complement(3772274..3773446) 1 NC_008702.1 Conserved hypothetical protein. Homology to pa1188 of P. aeruginosa of 40% (trembl|Q9I4F0). InterPro: CAIB/BAIF family (IPR003673). Pfam: CAIB/BAIF family. no signal peptide. no TMHs. TIGR00106: conserved hypothetical protein; hypothetical protein 3773446 4607950 azo3440 Azoarcus sp. BH72 hypothetical protein YP_934942.1 3772274 R 62928 CDS YP_934943.1 119899730 4607951 complement(3773552..3774784) 1 NC_008702.1 Phenylacetate-coenzyme A ligase (Phenylacetyl-CoA ligase) (PA-CoA ligase). CATALYZES THE ACTIVATION OF PHENYLACETIC ACID TO PHENYLACETYL-COA. InterPro: AMP-dependent synthetase and ligase; Function unclear; phenylacetate--CoA ligase 3774784 4607951 azo3441 Azoarcus sp. BH72 phenylacetate--CoA ligase YP_934943.1 3773552 R 62928 CDS YP_934944.1 119899731 4607952 complement(3774879..3775721) 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q7WQU7 (73%) and to trembl|Q89HM3 (54%). Pfam (PF00005): ABC transporter Smart (SM00382): AAA ATPase superfamily ProSite (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; ABC transporter ATP-binding protein 3775721 livF 4607952 livF Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934944.1 3774879 R 62928 CDS YP_934945.1 119899732 4609148 complement(3775809..3777131) 1 NC_008702.1 Conserved hypothetical ABC-type branched-chain amino acid transport systems, periplasmic component. Homology to Raeut03002728 of R. eutropha of 58% (gi|45519901|ref|ZP_00171452.1|(NCBI ENTREZ)). Pfam: Receptor family ligand binding region. This family includes extracellular ligand binding domains of a wide range of receptors. This family also includes the bacterial amino acid binding proteins of known structure. Signal peptide present. TMH in signal peptide.; Family membership; branched chain amino acid ABC transporter periplasmic protein 3777131 4609148 azo3443 Azoarcus sp. BH72 branched chain amino acid ABC transporter periplasmic protein YP_934945.1 3775809 R 62928 CDS YP_934946.1 119899733 4607953 complement(3777218..3778285) 1 NC_008702.1 Putative branched-chain amino acid transport permease. Homology to livM of E. coli of 27%. Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. Pfam: Branched-chain amino acid transport system no signal peptide probalbe 10 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 3778285 livM4 4607953 livM4 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_934946.1 3777218 R 62928 CDS YP_934947.1 119899734 4609161 complement(3778448..3779374) 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q881A7 (65%) and to sprot|LIVH_ECOLI (26%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting eight Tmhelix. SignalP reporting Signal peptide.; Specificity unclear; ABC transporter permease 3779374 4609161 azo3445 Azoarcus sp. BH72 ABC transporter permease YP_934947.1 3778448 R 62928 CDS YP_934948.1 119899735 4607954 complement(3779397..3780221) 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q881A6 (55%), to sprot|BRAF_PSEAE (34%) and to sprot|LIVG_ECOLI (33%). Pfam (PF00005): ABC transporter Smart (SM00382): AAA ATPase superfamily ProSite (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; ABC transporter ATP-binding protein 3780221 4607954 azo3446 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_934948.1 3779397 R 62928 CDS YP_934949.1 119899736 4607955 complement(3780259..3782268) 1 NC_008702.1 Function:-Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates cheY, the response regulator involved in flagellar movement and chemotaxis. Activity:- ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA Entry name SWISSPROT:ACSA_ECOLI InterPro:- IPR000873; AMP-bind. Pfam:- PF00501; AMP-binding; 1. Identities = 114/506 (22%) Number of predicted TMHs: 0; Family membership; putative Acetyl-coenzyme A synthetase 3782268 acS2 4607955 acS2 Azoarcus sp. BH72 putative Acetyl-coenzyme A synthetase YP_934949.1 3780259 R 62928 CDS YP_934950.1 119899737 4608273 3782641..3783468 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03001225 of Dechloromonas aromatica of 43% (gi|46141136|ref|ZP_00153001.2|(NBCI ENTREZ)). Pfam: Protein of unknown function. signal peptide. TMH in signal peptide; hypothetical protein 3783468 4608273 azo3448 Azoarcus sp. BH72 hypothetical protein YP_934950.1 3782641 D 62928 CDS YP_934951.1 119899738 4607956 complement(3783478..3784752) 1 NC_008702.1 Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway; glutamate-1-semialdehyde aminotransferase 3784752 hemL 4607956 hemL Azoarcus sp. BH72 glutamate-1-semialdehyde aminotransferase YP_934951.1 3783478 R 62928 CDS YP_934952.1 119899739 4608987 complement(3784828..3785448) 1 NC_008702.1 Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate to form thiamine monophosphate; thiamine-phosphate pyrophosphorylase 3785448 thiE 4608987 thiE Azoarcus sp. BH72 thiamine-phosphate pyrophosphorylase YP_934952.1 3784828 R 62928 CDS YP_934953.1 119899740 4609937 complement(3785441..3786283) 1 NC_008702.1 Phosphomethylpyrimidine kinase (HMP-phosphate kinase) (HMP-P kinase). catalyzes the phosphorylation of hmp-p to hmp-pp (by similarity) TIGRFAM: HMP-P_kinase: phosphomethylpyrimidine kinase; Specificity unclear; phosphomethylpyrimidine kinase 3786283 thiD2 4609937 thiD2 Azoarcus sp. BH72 phosphomethylpyrimidine kinase YP_934953.1 3785441 R 62928 CDS YP_934954.1 119899741 4609936 3786395..3786574 1 NC_008702.1 High confidence in function and specificity; RubB protein 3786574 rubB 4609936 rubB Azoarcus sp. BH72 RubB protein YP_934954.1 3786395 D 62928 CDS YP_934955.1 119899742 4609833 3786655..3787041 1 NC_008702.1 Pilus protein, Response_reg. Pfam: PF00072; Response_reg. SMART: SM00448; REC. PilG protein. IS INVOLVED IN PILUS BIOSYNTHESIS AND TWITCHING MOTILITY. MAY ACT AS A SINGLE-DOMAIN RESPONSE REGULATOR WHOSE FUNCTION MAY BE TO RECEIVE A CERTAIN ENVIRONMENTAL SIGNAL(S) AND THEN TRANSDUCE THAT SIGNAL TO THE PILUS ASSEMBLY MACHINERY VIA SPECIFIC PROTEIN-PROTEIN INTERACTIONS.; High confidence in function and specificity; pilus protein 3787041 pilG 4609833 pilG Azoarcus sp. BH72 pilus protein YP_934955.1 3786655 D 62928 CDS YP_934956.1 119899743 4609561 3787065..3787430 1 NC_008702.1 Pilus protein, Response_reg. Pfam: PF00072; Response_reg. SMART: SM00448; REC. PilH protein. MAY BE A PART OF A SIGNAL-TRANSDUCTION SYSTEM THAT REGULATES TWITCHING MOTILITY BY CONTROLLING PILUS FUNCTION (EXTENSION AND RETRACTION).; High confidence in function and specificity; pilus protein 3787430 pilH 4609561 pilH Azoarcus sp. BH72 pilus protein YP_934956.1 3787065 D 62928 CDS YP_934957.1 119899744 4609562 3787438..3787950 1 NC_008702.1 Probable pilus biogenesis protein, CheW. Protein pilI. MAY BE A PART OF A SIGNAL-TRANSDUCTION SYSTEM THAT REGULATES TWITCHING MOTILITY BY CONTROLLING PILUS FUNCTION (EXTENSION AND RETRACTION). InterPro: CheW-like domain; High confidence in function and specificity; pilus biogenesis protein 3787950 pilI 4609562 pilI Azoarcus sp. BH72 pilus biogenesis protein YP_934957.1 3787438 D 62928 CDS YP_934958.1 119899745 4609563 3788002..3790122 1 NC_008702.1 Probable pilus biogenesis protein,; High confidence in function and specificity; pilus biogenesis protein 3790122 pilJ 4609563 pilJ Azoarcus sp. BH72 pilus biogenesis protein YP_934958.1 3788002 D 62928 CDS YP_934959.1 119899746 4609564 3790157..3796255 1 NC_008702.1 Putative pilus biogenese protein, ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR002545; CheW. IPR004105; H-kinase_dim. IPR005467; His_kinase. IPR008207; Hpt. IPR008208; Hpt_N. IPR001789; Response_reg. Pfam: PF01584; CheW. PF02895; H-kinase_dim. PF02518; HATPase_c. PF01627; Hpt. PF00072; Response_reg. SMART: SM00260; CheW. SM00387; HATPase_c. SM00073; HPT. SM00448; REC. HTH reporting nucleic acid binding motif; Function unclear; putative pilus biogenese protein 3796255 pilL 4609564 pilL Azoarcus sp. BH72 putative pilus biogenese protein YP_934959.1 3790157 D 62928 CDS YP_934960.1 119899747 4609565 complement(3796292..3797803) 1 NC_008702.1 DNA photolyase (Photoreactivating enzyme). THIS ENZYME CATALYZES THE LIGHT-DEPENDENT MONOMERIZATION (300-600 NM) OF CYCLOBUTYL PYRIMIDINE DIMERS (IN CIS-SYN CONFIGURATION) WHICH ARE FORMED BETWEEN ADJACENT BASES ON THE SAME DNA STRAND UPON EXPOSURE TO ULTRAVIOLET RADIATION. TIGRFAM: phr2: deoxyribodipyrimidine photolyase.; High confidence in function and specificity; deoxyribodipyrimidine photo-lyase 3797803 phrB 4609565 phrB Azoarcus sp. BH72 deoxyribodipyrimidine photo-lyase YP_934960.1 3796292 R 62928 CDS YP_934961.1 119899748 4609553 3797871..3798437 1 NC_008702.1 Transcriptional regulator,; Specificity unclear; transcriptional regulator 3798437 algH 4609553 algH Azoarcus sp. BH72 transcriptional regulator YP_934961.1 3797871 D 62928 CDS YP_934962.1 119899749 4608297 3798430..3798900 1 NC_008702.1 similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function; Holliday junction resolvase-like protein 3798900 yqgF 4608297 yqgF Azoarcus sp. BH72 Holliday junction resolvase-like protein YP_934962.1 3798430 D 62928 CDS YP_934963.1 119899750 4610196 3798887..3799387 1 NC_008702.1 regulates pyrimidine biosynthesis by binding to the mRNA of the pyr genes, also has been shown to have uracil phosphoribosyltransferase activity; bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase 3799387 pyrR 4610196 pyrR Azoarcus sp. BH72 bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase YP_934963.1 3798887 D 62928 CDS YP_934964.1 119899751 4609675 3799380..3800339 1 NC_008702.1 catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis; aspartate carbamoyltransferase catalytic subunit 3800339 pyrB 4609675 pyrB Azoarcus sp. BH72 aspartate carbamoyltransferase catalytic subunit YP_934964.1 3799380 D 62928 CDS YP_934965.1 119899752 4609668 3800410..3801693 1 NC_008702.1 Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides; dihydroorotase 3801693 pyrX 4609668 pyrX Azoarcus sp. BH72 dihydroorotase YP_934965.1 3800410 D 62928 CDS YP_934966.1 119899753 4609676 complement(3801700..3801996) 1 NC_008702.1 Conserved hypothetical protein. Homology to vc0458 of V. clholerae of 48% (sprot:Y458_VIBCH). InterPro: DUF167 (IPR003748). Pfam: Uncharacterized ACR, YGGU family, COF1872. no signal peptide. no TMHs; hypothetical protein 3801996 yggU 4609676 yggU Azoarcus sp. BH72 hypothetical protein YP_934966.1 3801700 R 62928 CDS YP_934967.1 119899754 4610178 complement(3802001..3802582) 1 NC_008702.1 Conserved hypothetical YGGT-family protein. Homology to ne0394 of N. europae of 37% (trembl|Q82X94). This family consists of a repeat found in conserved hypothetical integral membrane proteins. The function of this region and the proteins which possess it is unknown. InterPro: YGGT family (IPR003425). Pfam: YGGT family. no signal peptide. 5 TMHs.; Conserved hypothetical protein; hypothetical protein 3802582 4610178 azo3465 Azoarcus sp. BH72 hypothetical protein YP_934967.1 3802001 R 62928 CDS YP_934968.1 119899755 4607957 complement(3802601..3803425) 1 NC_008702.1 catalyzes the formation of L-proline from pyrroline-5-carboxylate; pyrroline-5-carboxylate reductase 3803425 proC 4607957 proC Azoarcus sp. BH72 pyrroline-5-carboxylate reductase YP_934968.1 3802601 R 62928 CDS YP_934969.1 119899756 4609634 complement(3803499..3804212) 1 NC_008702.1 Concerved hypothetical protein. Homology to PA0394 of P. aeruginosa of 65% (sprot|Y394_PSEAE). InterPro: Uncharacterized pyridoxal-5-phosphate dependent enzyme family UPF0001 (IPR001608). Pfam: Uncharacterized protein family UPF0001. Tigrfam: TIGR00044: conserved hypothetical protein. no signal peptide. no TMHs; hypothetical protein 3804212 4609634 azo3467 Azoarcus sp. BH72 hypothetical protein YP_934969.1 3803499 R 62928 CDS YP_934970.1 119899757 4607958 3804339..3805379 1 NC_008702.1 Tfp retraction protein PilT, involved in the twitching motility mechanism together with PilU,; High confidence in function and specificity; twitching motility protein 3805379 pilT 4607958 pilT Azoarcus sp. BH72 twitching motility protein YP_934970.1 3804339 D 62928 CDS YP_934971.1 119899758 4609573 3805466..3806599 1 NC_008702.1 Tfp retraction protein PilU is involved in the Twitching Motility mechanism,; High confidence in function and specificity; twitching motility protein 3806599 pilU2 4609573 pilU2 Azoarcus sp. BH72 twitching motility protein YP_934971.1 3805466 D 62928 CDS YP_934972.1 119899759 4609575 3806788..3809505 1 NC_008702.1 Probable vgr-related protein ,44% identity(54% similarity) to TrEMBL; Q7P238.TrEMBL;Q8XRU3(44% identity). Signal Peptide Present. Has SMART;SM00438,Repressor of transcription;IPR000967, Znf_NFX1;This domain is presumed to be a zinc binding domain. The following pattern describes the zinc finger: C-X(1-6)-H-X-C-X3-C(H/C)-X(3-4)-(H/C)-X(1-10)-C,where X can be any amino acid, and numbers in brackets indicate the number of residues. The two position can be either his or cys. This domain is found in the human transcriptional repressor NK-X1, a repressor of HLA-DRA transcription; the Drosophila shuttle craft protein, which plays an essential role during the late stages of embryonic neurogenesis; and a yeast hypothetical protein YNL023C. Has PF04524, Protein of unknown function,DUF586; IPR006533,Rhs_Vgr;This family contains a conserved region in several bacterial proteins of unknown function.; High confidence in function and specificity; Vgr-related protein 3809505 vgrG 4609575 vgrG Azoarcus sp. BH72 Vgr-related protein YP_934972.1 3806788 D 62928 CDS YP_934973.1 119899760 4610093 3809502..3811634 1 NC_008702.1 Conserved hypothetical protein. Homology to RS02436 of R.solanacearum of 41% (trembl:Q8XUG9). No domains predicted. No signal peptide present. No TMH reported present.; hypothetical protein 3811634 4610093 azo3471 Azoarcus sp. BH72 hypothetical protein YP_934973.1 3809502 D 62928 CDS YP_934974.1 119899761 4607959 3811703..3813064 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS02435 of R. solanarcearum of 43% (trembl|Q8XUH0(SRS)). No domains predicted. Signal Peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 3813064 4607959 azo3472 Azoarcus sp. BH72 hypothetical protein YP_934974.1 3811703 D 62928 CDS YP_934975.1 119899762 4607960 3813157..3814398 1 NC_008702.1 Conserved hypothetical protein. Homology to RS02435 of R. solanacearum of 47% (trembl:Q8XUH0). No domains predicted. No signal peptide. No TMHs; hypothetical protein 3814398 4607960 azo3473 Azoarcus sp. BH72 hypothetical protein YP_934975.1 3813157 D 62928 CDS YP_934976.1 119899763 4607961 3814395..3815732 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS02435 of R. solanarcearum of 44% (trembl|Q8XUH0(SRS)). No domains predicted. Signal Peptide present. No TMH reported present; Conserved hypothetical protein; hypothetical protein 3815732 4607961 azo3474 Azoarcus sp. BH72 hypothetical protein YP_934976.1 3814395 D 62928 CDS YP_934977.1 119899764 4607962 complement(3815864..3817339) 1 NC_008702.1 Putative mcp-domain signal transduction protein,; Conserved hypothetical protein; MCP-domain-containing signal transduction protein 3817339 4607962 azo3475 Azoarcus sp. BH72 MCP-domain-containing signal transduction protein YP_934977.1 3815864 R 62928 CDS YP_934978.1 119899765 4607963 complement(3817314..3817835) 1 NC_008702.1 Putative signal-transduction sensor protein,; Conserved hypothetical protein; putative signal-transduction sensor protein 3817835 4607963 azo3476 Azoarcus sp. BH72 putative signal-transduction sensor protein YP_934978.1 3817314 R 62928 CDS YP_934979.1 119899766 4607964 complement(3817884..3819047) 1 NC_008702.1 hypothetical protein 3819047 4607964 azo3477 Azoarcus sp. BH72 hypothetical protein YP_934979.1 3817884 R 62928 CDS YP_934980.1 119899767 4607965 complement(3819301..3819513) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc2369 of R. solanacearum of 52% (trembl|Q8XWV1). no damians predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 3819513 4607965 azo3478 Azoarcus sp. BH72 hypothetical protein YP_934980.1 3819301 R 62928 CDS YP_934981.1 119899768 4607966 complement(3819510..3820550) 1 NC_008702.1 Putative formate dehydrogenase gamma subunit. Homology to fdhC of W. succinogenes of 21% (TREMBL:P28180). Formate dehydrogenase is a membrane-bound complex, formed of at least three different subunits; probable heterotetramer of two FDHA, one FDHB and one FDHC. FdhC probably anchors the enzyme in the membrane and IS PROBABLY THE CYTOCHROME B556(FDO) COMPONENT. no good domains signal peptide 5 TMHs; Family membership; putative formate dehydrogenase subunit gamma 3820550 fdhC 4607966 fdhC Azoarcus sp. BH72 putative formate dehydrogenase subunit gamma YP_934981.1 3819510 R 62928 CDS YP_934982.1 119899769 4608743 complement(3820561..3820794) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc2371 of R. solanacearum of 36% (trembl|Q8XWU9). no doamins predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 3820794 4608743 azo3480 Azoarcus sp. BH72 hypothetical protein YP_934982.1 3820561 R 62928 CDS YP_934983.1 119899770 4607967 complement(3820791..3821399) 1 NC_008702.1 probable formate dehydrogenase iron-sulfur subunit. Homology to fdhB of W. succinogenes of 63% (SWISSPROT:FDHB_WOLSU) THIS CHAIN IS AN ELECTRON TRANSFER UNIT CONTAINING 18 CYSTEINE RESIDUES 16 OF WHICH OCCUR IN THREE CLUSTERS. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450); 7Fe ferredoxin (IPR000813); 3Fe-4S ferredoxin (IPR001080) Pfam: 4Fe-4S binding domain no signal peptide no TMHs; High confidence in function and specificity; formate dehydrogenase iron-sulfur subunit 3821399 fdhB 4607967 fdhB Azoarcus sp. BH72 formate dehydrogenase iron-sulfur subunit YP_934983.1 3820791 R 62928 CDS YP_934984.1 119899771 4608742 complement(3821419..3824322) 1 NC_008702.1 Probable formate dehydrogenase alpha subunit. Homology to fdha of W. succinogenes of 44% (pir|S18213) The formate dehydrogenase catalyzes the oxidation of formate (produced from pyruvate during anaerobic growth) to carbon dioxide with the concomitant release of two electrons and two protons. The electrons are utilized mainly in the nitrate respiration by nitrate reductase InterPro: Prokaryotic molybdopterin oxidoreductases (IPR006655) Pfam: Molybdopterin oxidoreductase; Molydopterin dinucleotide binding domain singal peptide no TMHs; High confidence in function and specificity; formate dehydrogenase subunit alpha 3824322 fdhA2 4608742 fdhA2 Azoarcus sp. BH72 formate dehydrogenase subunit alpha YP_934984.1 3821419 R 62928 CDS YP_934985.1 119899772 4608741 complement(3824336..3824572) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc2374 of R. solanacearum of 39% (trembl|Q8XWU6). no domains predicted. signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 3824572 4608741 azo3483 Azoarcus sp. BH72 hypothetical protein YP_934985.1 3824336 R 62928 CDS YP_934986.1 119899773 4607968 complement(3824610..3825233) 1 NC_008702.1 Putative Chaperone protein TorD. Homology to torD of E. coli of 23% (sprot|TORD_ECOLI). This family consists of several bacterial TorD proteins. Many prokaryotic molybdoenzymes, for example the TMAO reductase (TorA) of Escherichia coli, require the insertion of a bis(molybdopterin guanine dinucleotide) molybdenum (bis(MGD)Mo) cofactor in its catalytic site to be active and translocated to the periplasm. The TorD chaperone increases apoTorA activation up to four-fold, allowing maturation of most of the apoprotein. Therefore TorD is involved in the first step of TorA maturation to make it competent to receive the cofactor. Pfam: Cytoplasmic chaperon no signal peptide no TMHs; Family membership; putative chaperone protein TorD 3825233 torD 4607968 torD Azoarcus sp. BH72 putative chaperone protein TorD YP_934986.1 3824610 R 62928 CDS YP_934987.1 119899774 4609964 complement(3825247..3827352) 1 NC_008702.1 Conserved hypothetical iron-sulfur binding protein. Homology to pb1470 of B. pertussis of 48% (trembl|Q7VY84). Transfer of electrons in a wide variety of metabolic reactions. InterPro: 4Fe-4S ferredoxin iron-sulfur binding domain (IPR001450). Pfam: 4Fe-4S binding domain. no signal peptide. no TMHs; Family membership; iron-sulfur cluster-binding protein 3827352 4609964 azo3485 Azoarcus sp. BH72 iron-sulfur cluster-binding protein YP_934987.1 3825247 R 62928 CDS YP_934988.1 119899775 4607969 complement(3827941..3828564) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS01168 of R.solanacearum of 40% (trembl:Q8XWU3). No domains predicted. No signal peptide or TMH reported present.; hypothetical protein 3828564 4607969 azo3486 Azoarcus sp. BH72 hypothetical protein YP_934988.1 3827941 R 62928 CDS YP_934989.1 119899776 4607970 complement(3828561..3829046) 1 NC_008702.1 Conserved hypothetical protein. Homology to BPP1929 of B.parapertussis of 34% (trembl:34%). No signal peptide or TMH reported present. No domains predicted.; hypothetical protein 3829046 4607970 azo3487 Azoarcus sp. BH72 hypothetical protein YP_934989.1 3828561 R 62928 CDS YP_934990.1 119899777 4607971 3829147..3830031 1 NC_008702.1 Putative formate dehydrogenase accessory protein. Homology to fdhD of W. succinogenes of 20% (sprot|FDHD_WOLSU). Necessary for formate dehydrogenase activity (By similarity). InterPro: Formate dehydrogenase subunit FdhD (IPR003786). Pfam: FdhD/NarQ family no signal peptide no TMHs; Family membership; putative formate dehydrogenase accessory protein 3830031 fdhD 4607971 fdhD Azoarcus sp. BH72 putative formate dehydrogenase accessory protein YP_934990.1 3829147 D 62928 CDS YP_934991.1 119899778 4608744 3830028..3830669 1 NC_008702.1 Probable molybdopterin-guanine dinucleotide biosynthesis protein A, 46% Identity to SwissProt;Q8Y0K5. TrEMBL;Q62LV3(44% Identity)Q63S71 Has PF01128,Uncharacterized protein family UPF0007;IPR001228 ISPD_synthase; The bacterial ispD protein catalyzes the third step of the deoxyxylulose-5-phosphate pathway (DXP) of isoprenoid biosynthesis; the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol from CTP and 2C-methyl-D-erythritol 4-phosphate.; molybdopterin-guanine dinucleotide biosynthesis protein A 3830669 mobA 4608744 mobA Azoarcus sp. BH72 molybdopterin-guanine dinucleotide biosynthesis protein A YP_934991.1 3830028 D 62928 CDS YP_934992.1 119899779 4609245 3830659..3831579 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA2947 of Azoarcus sp. EbN1 of 58% (gnl|keqq|eba:ebA2947(KEGG)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 3831579 4609245 azo3490 Azoarcus sp. BH72 hypothetical protein YP_934992.1 3830659 D 62928 CDS YP_934993.1 119899780 4607972 complement(3831592..3832095) 1 NC_008702.1 Molybdopterin-guanine dinucleotide biosynthesis protein B. MAY BIND THE GUANINE NUCLEOTIDE REQUIRED FOR THE SYNTHESIS OF MOLYBDOPTERIN GUANINE DINUCLEOTIDE. InterPro: ATP/GTP-binding site motif A (P-loop) TIGRFAM: mobB: molybdopterin-guanine dinucleotide; High confidence in function and specificity; molybdopterin-guanine dinucleotide biosynthesis protein B 3832095 mobB2 4607972 mobB2 Azoarcus sp. BH72 molybdopterin-guanine dinucleotide biosynthesis protein B YP_934993.1 3831592 R 62928 CDS YP_934994.1 119899781 4609247 complement(3832128..3834263) 1 NC_008702.1 Putative sensor histidine kinase, His_kinA_N. IPR009082; His_kin_homodim. IPR003660; HAMP. IPR003594; ATPbind_ATPase. Pfam: PF02518; HATPase_c. PF00512; HisKA. PF00672; HAMP. SMART: SM00387; HATPase_c. SM00388; HisKA. SM00304; HAMP. TMHMM reporting 2 transmembrane helices.; Specificity unclear; putative sensor histidine kinase 3834263 4609247 azo3492 Azoarcus sp. BH72 putative sensor histidine kinase YP_934994.1 3832128 R 62928 CDS YP_934995.1 119899782 4607973 complement(3834272..3835684) 1 NC_008702.1 Probable sigma-54 dependent response regulator,AAA_ATPase. IPR008931; FIS-like. IPR002197; HTH_Fis. IPR010114; NtrC. IPR001789; Response_reg. IPR008298; Res_reg_NtrC. IPR002078; Sig54_interact. Pfam: PF02954; HTH_8. PF00072; Response_reg. PF00158; Sigma54_activat. SMART: SM00382; AAA. SM00448; REC. HTH reporting reporting nucleic acid binding motif.; Family membership; sigma-54 dependent response regulator 3835684 sasR 4607973 sasR Azoarcus sp. BH72 sigma-54 dependent response regulator YP_934995.1 3834272 R 62928 CDS YP_934996.1 119899783 4609838 complement(3835728..3836138) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to CV0155 of Chromobacterium violaceum of 69% (trembl|Q7P1Q8). Has PF03653, Uncharacterised protein family (UPF0093);IPR005265, Cons_hypoth701:It appears the conserved hypothetical integral membrane proteins of this family are found only in gram negative bacteria and their function is unknown. No signal peptide. 4 TMHs; Conserved hypothetical protein; hypothetical protein 3836138 4609838 azo3494 Azoarcus sp. BH72 hypothetical protein YP_934996.1 3835728 R 62928 CDS YP_934997.1 119899784 4607974 complement(3836156..3837034) 1 NC_008702.1 55%; Specificity unclear; hypothetical protein 3837034 4607974 azo3495 Azoarcus sp. BH72 hypothetical protein YP_934997.1 3836156 R 62928 CDS YP_934998.1 119899785 4607975 3837056..3838075 1 NC_008702.1 catalyzes the formation of 3,6-dideoxy-D-glycero-D-glycero-4-hexulose; CDP-6-deoxy-delta-3,4-glucoseen reductase 3838075 ascD 4607975 ascD Azoarcus sp. BH72 CDP-6-deoxy-delta-3,4-glucoseen reductase YP_934998.1 3837056 D 62928 CDS YP_934999.1 119899786 4608356 3838095..3839231 1 NC_008702.1 conserved hypothetical regulator protein,; Conserved hypothetical protein; putative regulator protein 3839231 4608356 azo3497 Azoarcus sp. BH72 putative regulator protein YP_934999.1 3838095 D 62928 CDS YP_935000.1 119899787 4607976 3839343..3841346 1 NC_008702.1 Putative sensor protein, no significant homology. Best Hit SWISSPROT: sprot|ZRAS_SALTY (10% Salmonella typhimurium, ZraS) InterPro: IPR003594; ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR005467; His_kinase. Pfam: PF02518; HATPase_c. PF00512; HisKA. SMART: SM00387; HATPase_c. SM00388; HisKA. Signal P reporting signal peptide. Sensor protein zraS (EC 2.7.3.-). Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium (By similarity).; putative sensor protein 3841346 4607976 azo3498 Azoarcus sp. BH72 putative sensor protein YP_935000.1 3839343 D 62928 CDS YP_935001.1 119899788 4607977 3841404..3843584 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to Daro03001340 of Dechloromonas aromatica of 45% (gi|53730727|ref|ZP_00348932.1|(NBCI ENTREZ)). InterPro: IPR000160 GGDEF. IPR000014 PAS. IPR000700 PAS-assoc_C. IPR001789 Response_reg. IPR001633 EAL. Pfam: PF00990 GGDEF domain. PF00989 PAS domain. PF00563 EAL domain. PF00072 Response_reg. TIGRFAM: TIGR00229 PAS domain S-box. TIGR00254 putative diguanylate cyclase (GGDEF) domain.; Conserved hypothetical protein; putative signaling protein 3843584 4607977 azo3499 Azoarcus sp. BH72 putative signaling protein YP_935001.1 3841404 D 62928 CDS YP_935002.1 119899789 4607978 complement(3843766..3844074) 1 NC_008702.1 probable cytochrome c-552 precursor. Homology to cyt of N. europaea of 52% (AAB46987). ELECTRON DONOR FOR CYTOCHROME CD1 IN NITRITE AND NITRATE RESPIRATION. InterPro: Cytochrome c class I (IPR003088); cytochrome c,class ID (IPR002324) Pfam: cytochrome c signal peptide no TMHs; High confidence in function and specificity; cytochrome c-552 3844074 cyt2 4607978 cyt2 Azoarcus sp. BH72 cytochrome c-552 YP_935002.1 3843766 R 62928 CDS YP_935003.1 119899790 4608568 complement(3844213..3844836) 1 NC_008702.1 Urease accessory protein ureG. Homology to ureG of K. aerognes of 71% (AAA25154). Probably facilitates nickel incorporation. Pfam: HypB/UreG nucleotide-binding domain Tigrfam: ureG: urease accessory protein UreG no signal peptide no TMHs; High confidence in function and specificity; urease accessory protein UreG 3844836 ureG 4608568 ureG Azoarcus sp. BH72 urease accessory protein UreG YP_935003.1 3844213 R 62928 CDS YP_935004.1 119899791 4610075 complement(3845086..3845790) 1 NC_008702.1 Probable urease accessory protein ureF. Homology to ureF of K. aerogenes of 41% (sprot|UREF_KLEAE). UreF is proposed to modulate the activation process of urease by eliminating the binding of nickel irons to noncarbamylated protein InterPro: UreF (IPR002639) Pfam: ureF probable signal peptide no TMHs; High confidence in function and specificity; urease accessory protein UreF 3845790 ureF 4610075 ureF Azoarcus sp. BH72 urease accessory protein UreF YP_935004.1 3845086 R 62928 CDS YP_935005.1 119899792 4610074 complement(3845787..3846266) 1 NC_008702.1 involved in the assembly of the urease metallocenter; possible nickel donor; urease accessory protein UreE 3846266 ureE 4610074 ureE Azoarcus sp. BH72 urease accessory protein UreE YP_935005.1 3845787 R 62928 CDS YP_935006.1 119899793 4607979 complement(3846323..3848029) 1 NC_008702.1 ureases catalyze the hydrolysis of urea into ammonia and carbon dioxide; in Helicobacter pylori the ammonia released plays a key role in bacterial survival by neutralizing acids when colonizing the gastric mucosa; the holoenzyme is composed of 3 ureC (alpha) and 3 ureAB (gamma/beta) subunits; urease subunit alpha 3848029 ureC 4607979 ureC Azoarcus sp. BH72 urease subunit alpha YP_935006.1 3846323 R 62928 CDS YP_935007.1 119899794 4610072 complement(3848031..3848336) 1 NC_008702.1 Region start changed from 3848352 to 3848310 (-42 bases); urease subunit beta 3848336 4610072 azo3505 Azoarcus sp. BH72 urease subunit beta YP_935007.1 3848031 R 62928 CDS YP_935008.1 119899795 4607980 complement(3848368..3848667) 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03003730 of Dechloromonas aromatica of 40% (gi|46140350|ref|ZP_00203571.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; Family membership; hypothetical protein 3848667 4607980 azo3506 Azoarcus sp. BH72 hypothetical protein YP_935008.1 3848368 R 62928 CDS YP_935009.1 119899796 4607981 complement(3848714..3849016) 1 NC_008702.1 UreA, with UreB and UreC catalyzes the hydrolysis of urea into ammonia and carbon dioxide; nickel metalloenzyme; accessory proteins UreD, UreE, UreF, and UreG are necessary for assembly of the metallocenter; urease subunit gamma 3849016 ureA 4607981 ureA Azoarcus sp. BH72 urease subunit gamma YP_935009.1 3848714 R 62928 CDS YP_935010.1 119899797 4610071 complement(3849059..3849916) 1 NC_008702.1 Putative urease accessory protein ureD. PROBABLY FACILITATES NICKEL INCORPORATION. SEEMS TO BE ESSENTIAL FOR UREOLYSIS. InterPro: UreD urease accessory protein Pfam: UreeD urease accessory protein no signal peptide no TMHs; High confidence in function and specificity; putative urease accessory protein UreD 3849916 ureD 4610071 ureD Azoarcus sp. BH72 putative urease accessory protein UreD YP_935010.1 3849059 R 62928 CDS YP_935011.1 119899798 4610073 complement(3850094..3850939) 1 NC_008702.1 catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) in queuosine biosynthesis; 7-cyano-7-deazaguanine reductase 3850939 queF 4610073 queF Azoarcus sp. BH72 7-cyano-7-deazaguanine reductase YP_935011.1 3850094 R 62928 CDS YP_935012.1 119899799 4608866 complement(3850971..3853019) 1 NC_008702.1 InterPro: Sulfatase Membrane protein; Specificity unclear; sulfatase family protein 3853019 4608866 azo3510 Azoarcus sp. BH72 sulfatase family protein YP_935012.1 3850971 R 62928 CDS YP_935013.1 119899800 4607982 complement(3853231..3854097) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA0234 of P.aeruginosa of 43% (trembl:Q9I6Q4). No domains predicted. Signal peptide present. No TMH present.; Conserved hypothetical protein; hypothetical protein 3854097 4607982 azo3511 Azoarcus sp. BH72 hypothetical protein YP_935013.1 3853231 R 62928 CDS YP_935014.1 119899801 4607983 3854510..3855544 1 NC_008702.1 Part of the ABC transporter complex ssuABC involved in aliphatic sulfonates import.Similar to the putative periplasmic-binding protein ssuA precursor in E.coli. SSUA_BACSU:P40400. InterPro:IPR000437; Prok_lipoprot_S. IPR001638; SBP_bac_3. SMART: SM00062; PBPb; 1. TMhelix:1. Signal peptide: present.; Specificity unclear; putative periplasmic-binding protein 3855544 ssuA 4607983 ssuA Azoarcus sp. BH72 putative periplasmic-binding protein YP_935014.1 3854510 D 62928 CDS YP_935015.1 119899802 4609898 3855597..3856376 1 NC_008702.1 Region start changed from 3855391 to 3855571 (-180 bases); putative aliphatic sulfonates transport systemATP-binding protein 3856376 4609898 azo3513 Azoarcus sp. BH72 putative aliphatic sulfonates transport systemATP-binding protein YP_935015.1 3855597 D 62928 CDS YP_935016.1 119899803 4607984 3856392..3857165 1 NC_008702.1 Part of the ABC transporter complex ssuABC involved in aliphatic sulfonates import. Probably responsible for the translocation of the substrate across the membrane.26% Similar to the aliphatic sulfonates permease, ssuC in E.coli. SWISSPROT:SSUC_ECOLI: P75851 InterPro:IPR000515; BPD_transp. Pfam:PF00528; BPD_transp; 1. InterPro:PF00528: Binding-protein-dependent transport systems inner membrane component. TMhelix:6; Specificity unclear; putative aliphatic sulfonates transport systempermease 3857165 ssuC 4607984 ssuC Azoarcus sp. BH72 putative aliphatic sulfonates transport systempermease YP_935016.1 3856392 D 62928 CDS YP_935017.1 119899804 4609899 complement(3857523..3857933) 1 NC_008702.1 Putative transcriptional regulatory protein,; Conserved hypothetical protein; putative transcriptional regulator 3857933 4609899 azo3515 Azoarcus sp. BH72 putative transcriptional regulator YP_935017.1 3857523 R 62928 CDS YP_935018.1 119899805 4607985 3858050..3858604 1 NC_008702.1 Flavoprotein wrbA3. TREMBL:Q8PGA8: 53% identity,66% similarity. wrbA3 is 26% identical to wrbA1 and 23% identical to wrbA2 InterPro: IPR008254; Flav_nitox_synth. IPR001226; Flavodoxin. Pfam:PF00258: flavodoxin; TIGR00301: NADP oxidoreductase coenzyme Number of transmembrane helices:0; High confidence in function and specificity; tryptophan repressor binding protein 3858604 wrbA3 4607985 wrbA3 Azoarcus sp. BH72 tryptophan repressor binding protein YP_935018.1 3858050 D 62928 CDS YP_935019.1 119899806 4610114 complement(3858601..3860079) 1 NC_008702.1 catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation; cobyric acid synthase 3860079 4610114 azo3517 Azoarcus sp. BH72 cobyric acid synthase YP_935019.1 3858601 R 62928 CDS YP_935020.1 119899807 4607986 3860126..3861145 1 NC_008702.1 CobD; CbiD in Salmonella; converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group; cobalamin biosynthesis protein 3861145 cobD 4607986 cobD Azoarcus sp. BH72 cobalamin biosynthesis protein YP_935020.1 3860126 D 62928 CDS YP_935021.1 119899808 4608502 3861138..3862133 1 NC_008702.1 Cobalamin biosynthesis protein, CobC. CobC is probable involved in the conversion of cobyric acid to cobinamide.Similar to pir|B83486 (48%) and to sprot|COBC_PSEDE (42%). InterPro (PF00155): Aminotransferases class-I and II; Specificity unclear; cobalamin biosynthesis protein 3862133 cobC 4608502 cobC Azoarcus sp. BH72 cobalamin biosynthesis protein YP_935021.1 3861138 D 62928 CDS YP_935022.1 119899809 4608500 3862136..3863032 1 NC_008702.1 Vitamin B12 transport protein btuF precursor. 34% Similar to BtuF from E.coli. Part of the binding-protein-dependent transport system for vitamin B12. Acts with btuCD to transport vitamin B12 across the inner membrane. SWISSPROT:BTUF_ECOLI:P37028 InterPro:IPR002491; Peripla_BP. Pfam: PF01497; Peripla_BP_2; 1. Signal peptide: present. Contains 1 iron siderophore/cobalamin periplasmic- binding domain.; High confidence in function and specificity; putative vitamin B12 transport protein 3863032 btuF 4608500 btuF Azoarcus sp. BH72 putative vitamin B12 transport protein YP_935022.1 3862136 D 62928 CDS YP_935023.1 119899810 4608407 complement(3863039..3864103) 1 NC_008702.1 Conserved hypothetical peptidase. Homology to ebA3686 of Azoarcus sp. EbN1 of 45% (gnl|keqq|eba:ebA3686(KEGG)) . Pfam: Peptidase family M48. signal peptide. 1 TMHs; Conserved hypothetical protein; peptidase 3864103 4608407 azo3521 Azoarcus sp. BH72 peptidase YP_935023.1 3863039 R 62928 CDS YP_935024.1 119899811 4607987 3864452..3866065 1 NC_008702.1 ATP-dependent RNA helicase rhlB (EC 3.6.1.-). Can carry out ATP-dependent unwinding of double stranded RNA. Has a role in RNA decay. Involved in the RNA degradosome a multi-enzyme complex important in RNA processing and messenger RNA degradation.; High confidence in function and specificity; putative ATP-dependent RNA helicase 3866065 rhlE3 4607987 rhlE3 Azoarcus sp. BH72 putative ATP-dependent RNA helicase YP_935024.1 3864452 D 62928 CDS YP_935025.1 119899812 4609718 complement(3866142..3867860) 1 NC_008702.1 Alpha-isopropylmalate synthase catalyses the first step in the biosynthesis of leucine, the condensation of acetyl-CoA and alpha- ketoisovalerate to form 2-isopropylmalate synthase. Similar to sprot|LEU1_PSEAE (59%) and to sprot|LE12_RALSO (68%). Pfam (PF00682): HMG-CoA Lyase-like family; High confidence in function and specificity; 2-isopropylmalate synthase 3867860 leuA2 4609718 leuA2 Azoarcus sp. BH72 2-isopropylmalate synthase YP_935025.1 3866142 R 62928 CDS YP_935026.1 119899813 4609130 complement(3868239..3868895) 1 NC_008702.1 Conserved hypothetical nitroreductase. Homology to cv1555 of C. violaceum of 72% (trembl|Q7NXS1). Involved in the reduction of nitrogen containing compounds. InterPro: Nitroreductase family (IPR000415). Pfam: Nitroreductase family. no signal peptide. no TMHs; Conserved hypothetical protein; nitroreductase 3868895 4609130 azo3524 Azoarcus sp. BH72 nitroreductase YP_935026.1 3868239 R 62928 CDS YP_935027.1 119899814 4607988 complement(3868933..3869736) 1 NC_008702.1 TREMBL:Q7NZN8 (43% identity, 53% similarity).; hypothetical protein 3869736 4607988 azo3525 Azoarcus sp. BH72 hypothetical protein YP_935027.1 3868933 R 62928 CDS YP_935028.1 119899815 4607989 3869994..3870761 1 NC_008702.1 Conserved hypothetical flavoprotein. Homology to tde2498 of T. denticola of 46% (tremblnew|AAS13015). InterPro: Metallo-beta-lactamase superfamily (IPR001279). Pfam: Metallo-beta-lactamase superfamily. no signal peptide. no TMHs; Conserved hypothetical protein; flavoprotein 3870761 4607989 azo3526 Azoarcus sp. BH72 flavoprotein YP_935028.1 3869994 D 62928 CDS YP_935029.1 119899816 4607990 3870767..3872938 1 NC_008702.1 GGDEF/EAL-domain containing protein; diguanylate cyclase 3872938 4607990 azo3527 Azoarcus sp. BH72 diguanylate cyclase YP_935029.1 3870767 D 62928 CDS YP_935030.1 119899817 4607991 3873423..3874049 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to blr3274 of B. japonicum of 33% (gnl|keqq|bja:blr3274(KEGG)). Pfam: weak hit to CbiM, an integral membrane protein involved in cobalamin synthesis. TMHMM reporting 5 transmembrane helices. No signal peptide.; Conserved hypothetical protein; hypothetical protein 3874049 4607991 azo3528 Azoarcus sp. BH72 hypothetical protein YP_935030.1 3873423 D 62928 CDS YP_935031.1 119899818 4607992 3874055..3874870 1 NC_008702.1 Probable Precorrin-4 C11-methyltransferase,47% Identity to TrEMBL;Q8GDX1, O26702. SwissProt;O87696. Has PF00590, Tetrapyrrole (Corrin/Porphyrin) Methylases;IPR000878 Cor/por_Metransf; This family uses S-AdoMet in the methylation of diverse substrates. This family includes a related group of bacterial proteins of unknown function, including P45528. This family includes the methylase Dipthine synthase. Uroporphyrin-III C-methyltransferase (EC: 2.1.1.107) (SUMT)catalyzes the transfer of two methyl groups from S-adenosyl-L-methionine to the C-2 and C-7 atoms of uroporphyrinogen III to yield precorrin-2 via the intermediate formation of precorrin-1. SUMT is the first enzyme specific to the cobalamin pathway and precorrin-2 is a common intermediate in the biosynthesis of corrinoids such as vitamin B12, siroheme and coenzyme F430. The sequences of SUMT from a variety of bacterial and archaeal species are currently available. In species such as Bacillus megaterium (gene cobA),Pseudomonas denitrificans (cobA) or Methanobacterium ivanovii (gene corA) SUMT is a protein of about 25 to 30 kD. In Escherichia coli and related bacteria, the cysG protein, which is involved in the biosynthesis of siroheme, is a multifunctional protein composed of a N-terminal domain, probably involved in transforming precorrin-2 into siroheme, and a C-terminal domain which has SUMT activity.; precorrin-4 C(11)-methyltransferase 3874870 4607992 azo3529 Azoarcus sp. BH72 precorrin-4 C(11)-methyltransferase YP_935031.1 3874055 D 62928 CDS YP_935032.1 119899819 4607993 3874867..3875796 1 NC_008702.1 CbiX conserved hypothetical protein. Function unknown. Cobalamin biosynthesis pathway.; Function unclear; hypothetical protein 3875796 cbiX 4607993 cbiX Azoarcus sp. BH72 hypothetical protein YP_935032.1 3874867 D 62928 CDS YP_935033.1 119899820 4608435 3875800..3876477 1 NC_008702.1 Precorrin-8X methylmutase . CATALYZES THE CONVERSION OF PRECORRIN-8X TO HYDROGENOBYRINIC ACID; A Cobalamin biosynthesis precorrin isomerase. METHYL MIGRATION REACTION DURING THE TRANSFORMATION OF PRECORRIN-3 TO FORM COBYRINIC ACID (BY SIMILARITY). InterPro: Precorrin-8X methylmutase CbiC/CobH TIGRFAM: redox_disulf_1: redox-active disulfide; High confidence in function and specificity; precorrin-8X methylmutase 3876477 cbiC 4608435 cbiC Azoarcus sp. BH72 precorrin-8X methylmutase YP_935033.1 3875800 D 62928 CDS YP_935034.1 119899821 4608424 3876482..3877690 1 NC_008702.1 Catalyzes the methylation of C-1 in cobalt-precorrin-5 and the subsequent extrusion of acetic acid from the resulting intermediate to form cobalt-precorrin-6A; cobalt-precorrin-6A synthase 3877690 cbiD 4608424 cbiD Azoarcus sp. BH72 cobalt-precorrin-6A synthase YP_935034.1 3876482 D 62928 CDS YP_935035.1 119899822 4608425 3877680..3878990 1 NC_008702.1 Precorrin-6 methyltransferase (Precorrin-6Y methylase). CATALYZES THE METHYLATION OF BOTH C-5 AND C-15 IN PRECORRIN-6Y TO FORM PRECORRIN-8X. InterPro: Uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase; High confidence in function and specificity; precorrin-6Y C(5,15)-methyltransferase 3878990 cbiE 4608425 cbiE Azoarcus sp. BH72 precorrin-6Y C(5,15)-methyltransferase YP_935035.1 3877680 D 62928 CDS YP_935036.1 119899823 4608426 3878980..3879933 1 NC_008702.1 Precorrin-2 C20-methyltransferase (EC 2.1.1.130) (S-adenosyl-L- methionine:precorrin-2 methyltransferase) (SP2MT). METHYLATES PRECORRIN-2 AT THE C-20 POSITION TO PRODUCE PRECORRIN-3A.; High confidence in function and specificity; precorrin-2 C(20)-methyltransferase 3879933 cbiL 4608426 cbiL Azoarcus sp. BH72 precorrin-2 C(20)-methyltransferase YP_935036.1 3878980 D 62928 CDS YP_935037.1 119899824 4608431 3880286..3881062 1 NC_008702.1 putative Cobalamin (vitamin B12) biosynthesis CbiG protein, truncated. Homology to the N-terminus of cbiG of b. magaterium of 39% (gi|3724046|emb|CAA04315.1|(nr)). Pfam: CibG, CbiG proteins are required for cobalamin (vitamin B 12 ) biosynthesis. IPR012407: Cobalamin (vitamin B12) biosynthesis CbiG. CbiG is one of the enzymes specific to the anaerobic route of cobalamin biosynthesis e.g., in Salmonella typhimurium. While its specific role in this pathway is not known yet, it has been speculated that CbiG may participate in the conversion of cobalt-precorrin 5 into cobalt-precorrin 6. This step requires opening of the delta-lactone ring,excision of acetaldehyde and C-1 methylation. Alternatively, it has been suggested that it may participate in ring contraction. no signal peptide. no TMHs; Function unclear; putative cobalamin (vitamin B12) biosynthesis CbiG protein, truncated 3881062 cbiGa 4608431 cbiGa Azoarcus sp. BH72 putative cobalamin (vitamin B12) biosynthesis CbiG protein, truncated YP_935037.1 3880286 D 62928 CDS YP_935038.1 119899825 4608427 3881071..3881472 1 NC_008702.1 C-terminal part of CbiG protein. Cobalamin biosynthesis pathway.Precorrin methylase; High confidence in function and specificity; putative cobalamin (vitamin B12) biosynthesis CbiG protein, truncated 3881472 cbiGb 4608427 cbiGb Azoarcus sp. BH72 putative cobalamin (vitamin B12) biosynthesis CbiG protein, truncated YP_935038.1 3881071 D 62928 CDS YP_935039.1 119899826 4608428 3881469..3882437 1 NC_008702.1 Precorrin-3B C17- methyltransferase. METHYLTRANSFERASE THAT CATALYZES THE METHYLATION OF C-17 IN PRECORRIN-3B TO FORM PRECORRIN-4 (BY SIMILARITY). InterPro: Uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase; High confidence in function and specificity; precorrin-3B C(17)-methyltransferase 3882437 cbiH 4608428 cbiH Azoarcus sp. BH72 precorrin-3B C(17)-methyltransferase YP_935039.1 3881469 D 62928 CDS YP_935040.1 119899827 4608429 3882434..3882826 1 NC_008702.1 Conserved hypothetical ferredoxin. Homology to rsp0627 of R. solanacearum of 46% (trembl|Q8XS54). Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. no signal peptide. no TMHs; Conserved hypothetical protein; ferredoxin 3882826 4608429 azo3538 Azoarcus sp. BH72 ferredoxin YP_935040.1 3882434 D 62928 CDS YP_935041.1 119899828 4607994 complement(3883237..3883746) 1 NC_008702.1 Hypothetical secreted protein. no homology of the entire protein to the data bank. no domains predicted. signal peptide. no TMHs; hypothetical protein 3883746 4607994 azo3539 Azoarcus sp. BH72 hypothetical protein YP_935041.1 3883237 R 62928 CDS YP_935042.1 119899829 4607995 3883931..3885445 1 NC_008702.1 Conserved hypothetical transcriptional regulatory protein. Homology to rsc0599 of R. solanacearum of 63% (trembl|Q8Y1U0). InterPro: Bacterial regulatory proteins GntR family (IPR000524). Pfam: Bacterial regulatory protein, gntR family; Aminotransferase classI and II. no signal peptide. no TMHs; Conserved hypothetical protein; putative transcriptional regulator 3885445 4607995 azo3540 Azoarcus sp. BH72 putative transcriptional regulator YP_935042.1 3883931 D 62928 CDS YP_935043.1 119899830 4607996 complement(3885550..3886434) 1 NC_008702.1 Hypothetical methyltransferase. no Homology of the entire protein with the data bank. Pfam: Putative methyltransferase This is a family of hypothetical proteins which are putative methyltransferases. The aligned region contains the GXGXG S-AdoMet binding site suggesting a putative methyltransferase activity. Interpro: SAM (and some other nucleotide) binding motif(IPR000051) no signal peptide no TMHs; methyltransferase 3886434 4607996 azo3541 Azoarcus sp. BH72 methyltransferase YP_935043.1 3885550 R 62928 CDS YP_935044.1 119899831 4607997 complement(3886435..3887310) 1 NC_008702.1 Conserved hypothetical glutathione transferase. Homology to psto0703 of P. syringae of 74% (trembl|Q889P6). Bacterial GSTs of known function often have a specific, growth-supporting role in biodegradative metabolism Pfam. Glutathione S-transferase, N-terminal; Glutathione S-transferase, C-terminal. no signal peptide. no TMHs; Family membership; glutathione transferase 3887310 4607997 azo3542 Azoarcus sp. BH72 glutathione transferase YP_935044.1 3886435 R 62928 CDS YP_935045.1 119899832 4607998 complement(3887435..3888298) 1 NC_008702.1 Hypothetical secreted protein. No homology of the entire protein to the data bank. Pfam: DUF1555 (domain of unknown function). signal peptide. no TMHs; hypothetical protein 3888298 4607998 azo3543 Azoarcus sp. BH72 hypothetical protein YP_935045.1 3887435 R 62928 CDS YP_935046.1 119899833 4607999 3888597..3889892 1 NC_008702.1 probable 4-hydroxybutyrate coenzyme A transferase. Homology to cat2 of C. kluyveri of 43% (sprot|CAT2_CLOKL) InterPro: Acetyl-CoA hydrolase/transferase (IPR003702) Pfam: Acetyl-CoA hydrolase/transferase no signal peptide no TMHS; Family membership; 4-hydroxybutyrate CoA-transferase 3889892 4607999 azo3544 Azoarcus sp. BH72 4-hydroxybutyrate CoA-transferase YP_935046.1 3888597 D 62928 CDS YP_935047.1 119899834 4608000 3889954..3890610 1 NC_008702.1 Conserved hypothetical transport membrane protein. Homology to rsc1520 of R. solanacearum of 59% (trembl|Q8XZ84). Pfam: LysE type translocator. Tigrfam: 2A76: Homoserine/Threonine efflux protein. no signal peptide. probable 6 TMHs; Family membership; leucine export protein LeuE 3890610 yeaS 4608000 yeaS Azoarcus sp. BH72 leucine export protein LeuE YP_935047.1 3889954 D 62928 CDS YP_935048.1 119899835 4610166 complement(3890614..3892104) 1 NC_008702.1 Probable carboxypeptidase TAQ. Homology to ctaQ of T. aquaticus of 40% (SWISSPROT:CTAQ_THEAQ) Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline). Pfam: carboxypeptidas Taq (M32) metallopep no signal peptide no TMHs; Family membership; carboxypeptidase TAQ 3892104 ctaQ 4610166 ctaQ Azoarcus sp. BH72 carboxypeptidase TAQ YP_935048.1 3890614 R 62928 CDS YP_935049.1 119899836 4608540 3892274..3892729 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. signal peptide present.; hypothetical protein 3892729 4608540 azo3547 Azoarcus sp. BH72 hypothetical protein YP_935049.1 3892274 D 62928 CDS YP_935050.1 119899837 4608001 3892726..3893553 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to cv0063 of C. violaceum of 41% (trembl|Q7P1Z9(SRS)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 3893553 4608001 azo3548 Azoarcus sp. BH72 hypothetical protein YP_935050.1 3892726 D 62928 CDS YP_935051.1 119899838 4608002 complement(3893550..3894416) 1 NC_008702.1 catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine; phosphatidylserine decarboxylase 3894416 psd 4608002 psd Azoarcus sp. BH72 phosphatidylserine decarboxylase YP_935051.1 3893550 R 62928 CDS YP_935052.1 119899839 4609638 complement(3894532..3895827) 1 NC_008702.1 responsible for the amidation of carboxylic groups at position A and C of cobyrinic acid or hydrogenobrynic acid; cobyrinic acid a,c-diamide synthase 3895827 cobB 4609638 cobB Azoarcus sp. BH72 cobyrinic acid a,c-diamide synthase YP_935052.1 3894532 R 62928 CDS YP_935053.1 119899840 4608499 complement(3895824..3896717) 1 NC_008702.1 Uroporphyrin-III C-methyltransferase (Urogen III methylase) (SUMT) (Uroporphyrinogen III methylase) (UROM). TIGRFAM: dph5: diphthine synthase; High confidence in function and specificity; uroporphyrin-III C-methyltransferase 3896717 cobA2 4608499 cobA2 Azoarcus sp. BH72 uroporphyrin-III C-methyltransferase YP_935053.1 3895824 R 62928 CDS YP_935054.1 119899841 4608498 complement(3896722..3897342) 1 NC_008702.1 catalyzes the formation of adenosylcob(III)yrinic acid a,c-diamide from cob(I)yrinic acid a,c-diamide; cob(I)yrinic acid a,c-diamide adenosyltransferase 3897342 cobO 4608498 cobO Azoarcus sp. BH72 cob(I)yrinic acid a,c-diamide adenosyltransferase YP_935054.1 3896722 R 62928 CDS YP_935055.1 119899842 4608503 complement(3897371..3898168) 1 NC_008702.1 37% Similar to the iron(III)-hydroxamate transport membrane protein, fhuC in E.coli.Part of the ABC transporter complex fhuACDB involved in iron(III)-hydroxamate import. Probably responsible for energy coupling to the transport system. SWISSPROT:FHUC_ECOLI:P07821 InterPro:IPR003593; AAA_ATPase.IPR003439; ABC_transporter. Pfam:PF00005; ABC_tran; 1.; High confidence in function and specificity; putative ABC-type transporter ATPase component 3898168 4608503 azo3553 Azoarcus sp. BH72 putative ABC-type transporter ATPase component YP_935055.1 3897371 R 62928 CDS YP_935056.1 119899843 4608003 complement(3898168..3899148) 1 NC_008702.1 Region start changed from 3899167 to 3899122 (-45 bases); putative ABC transporter system, permease 3899148 4608003 azo3554 Azoarcus sp. BH72 putative ABC transporter system, permease YP_935056.1 3898168 R 62928 CDS YP_935057.1 119899844 4608004 complement(3899200..3901071) 1 NC_008702.1 COBALAMIN RECEPTOR PROTEIN.; Specificity unclear; TonB-dependent receptor 3901071 4608004 azo3555 Azoarcus sp. BH72 TonB-dependent receptor YP_935057.1 3899200 R 62928 CDS YP_935058.1 119899845 4608005 complement(3901150..3903090) 1 NC_008702.1 Putative outer membrane TonB-dependent receptor,probably involved in iron metabolism. 20% Similar to the IrgA enterobactine outer membrane receptor in V. cholerae. INVOLVED IN THE INITIAL STEP OF IRON UPTAKE BY BINDING FERRIC VIBRIOBACTIN AN IRON CHELATIN SIDEROPHORE THAT ALLOWS V.CHOLERAE TO EXTRACT IRON FROM THE ENVIRONMENT. SWISSPROT:IRGA_VIBCH:P27772. 21% TonB_boxC. Pfam:PF00593; TonB_dep_Rec; 1.; Specificity unclear; putative TonB-dependent receptor 3903090 4608005 azo3556 Azoarcus sp. BH72 putative TonB-dependent receptor YP_935058.1 3901150 R 62928 CDS YP_935059.1 119899846 4608006 3903450..3904043 1 NC_008702.1 Bifunctional cobalamin biosynthesis protein cobU [Includes: Cobinamide kinase; Cobinamide phosphate guanylyltransferase]. ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate. InterPro: Cobalbumin biosynthesis enzyme; High confidence in function and specificity; cobinamide kinase/cobinamide phosphate guanylyltransferase 3904043 cobU 4608006 cobU Azoarcus sp. BH72 cobinamide kinase/cobinamide phosphate guanylyltransferase YP_935059.1 3903450 D 62928 CDS YP_935060.1 119899847 4608506 complement(3904044..3905105) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to gsu0462 of G. sulfurreducens of 41% (AAR33794). probable 2 TMHs. no signal peptide. No domains predicted.; Conserved hypothetical protein; hypothetical protein 3905105 4608506 azo3558 Azoarcus sp. BH72 hypothetical protein YP_935060.1 3904044 R 62928 CDS YP_935061.1 119899848 4608007 complement(3905102..3906664) 1 NC_008702.1 Hypothetical membrane protein. No homology of the whole protein to the data bank. Has PF05987, Bacterial protein of unknown function (DUF898);IPR010295 ; This family consists of several bacterial proteins of unknown function. Some of the family members are described as putative membrane proteins. No signal peptide 6 TMHs; hypothetical protein 3906664 4608007 azo3559 Azoarcus sp. BH72 hypothetical protein YP_935061.1 3905102 R 62928 CDS YP_935062.1 119899849 4608008 3906932..3907978 1 NC_008702.1 catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide and 5,6-dimethylbenzimidazole; nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase 3907978 cobT 4608008 cobT Azoarcus sp. BH72 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase YP_935062.1 3906932 D 62928 CDS YP_935063.1 119899850 4608505 3907990..3908760 1 NC_008702.1 catalyzes the formation of adenosylcobalamin from Ado-cobinamide-GDP and alpha-ribazole; cobalamin synthase 3908760 cobS 4608505 cobS Azoarcus sp. BH72 cobalamin synthase YP_935063.1 3907990 D 62928 CDS YP_935064.1 119899851 4608504 3908745..3909296 1 NC_008702.1 Alpha-ribazole-5-phosphate phosphatase. Converts N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole into N1-alpha-D-ribosyl-5,6-dimethylbenzimidazole; involved in the assembly of the nucleotide loop of cobalamin. 27% PG/BPGM_mutase. Pfam:PF00300; PGAM; 1.; High confidence in function and specificity; putative alpha-ribazole phosphatase 3909296 4608504 azo3562 Azoarcus sp. BH72 putative alpha-ribazole phosphatase YP_935064.1 3908745 D 62928 CDS YP_935065.1 119899852 4608009 3909437..3910396 1 NC_008702.1 ADP-heptose synthase (EC 2.7.-.-).; High confidence in function and specificity; ADP-heptose synthase 3910396 waaE2 4608009 waaE2 Azoarcus sp. BH72 ADP-heptose synthase YP_935065.1 3909437 D 62928 CDS YP_935066.1 119899853 4610104 3910439..3911416 1 NC_008702.1 59% Epimerase_Dh.IPR000205; NAD_BS. Pfam:PF01370; Epimerase; 1. Non-secretory protein.; High confidence in function and specificity; putative ADP-L-glycero-D-mannoheptose-6-epimerase 3911416 rfaD 4610104 rfaD Azoarcus sp. BH72 putative ADP-L-glycero-D-mannoheptose-6-epimerase YP_935066.1 3910439 D 62928 CDS YP_935067.1 119899854 4609715 3911413..3912468 1 NC_008702.1 ADP-heptose--LPS heptosyltransferase II (EC 2.-.-.-).; High confidence in function and specificity; ADP-heptose--LPS heptosyltransferase II 3912468 waaF2 4609715 waaF2 Azoarcus sp. BH72 ADP-heptose--LPS heptosyltransferase II YP_935067.1 3911413 D 62928 CDS YP_935068.1 119899855 4610106 3912476..3913567 1 NC_008702.1 Lipopolysaccharide heptosyltransferase-1 (EC 2.-.-.-). HEPTOSE TRANSFER TO THE LIPOPOLYSACCHARIDE CORE. IT TRANSFERS THE INNNERMOST HEPTOSE TO [4-P](3-DEOXY-D-MANNO-OCTULOSONIC ACID)2-IVA. S18: ribosomal protein S18; High confidence in function and specificity; lipopolysaccharide heptosyltransferase I 3913567 waaC2 4610106 waaC2 Azoarcus sp. BH72 lipopolysaccharide heptosyltransferase I YP_935068.1 3912476 D 62928 CDS YP_935069.1 119899856 4610102 3913567..3914700 1 NC_008702.1 Region start changed from 3913496 to 3913541 (-45 bases); glucosyltransferase I 3914700 4610102 azo3567 Azoarcus sp. BH72 glucosyltransferase I YP_935069.1 3913567 D 62928 CDS YP_935070.1 119899857 4608010 3914697..3915533 1 NC_008702.1 Lipopolysaccharide core biosynthesis protein rfaP. Specific function unclear. INVOLVED IN ATTACHMENT OF PHOSPHATE-CONTAINING SUBSTITUENTS TO THE INNER CORE.; lipopolysaccharide core biosynthesis protein 3915533 waaP1 4608010 waaP1 Azoarcus sp. BH72 lipopolysaccharide core biosynthesis protein YP_935070.1 3914697 D 62928 CDS YP_935071.1 119899858 4610107 3915530..3916279 1 NC_008702.1 Lipopolysaccharide core biosynthesis protein rfaP. INVOLVED IN ATTACHMENT OF PHOSPHATE-CONTAINING SUBSTITUENTS TO THE INNER CORE. Specific function unclear.; lipopolysaccharide core biosynthesis protein 3916279 waaP2 4610107 waaP2 Azoarcus sp. BH72 lipopolysaccharide core biosynthesis protein YP_935071.1 3915530 D 62928 CDS YP_935072.1 119899859 4610108 3916293..3917066 1 NC_008702.1 Lipopolysaccharide core biosynthesis protein. INVOLVED IN ATTACHMENT OF PHOSPHATE-CONTAINING SUBSTITUENTS TO THE INNER CORE. Specific function unclear.; lipopolysaccharide core biosynthesis protein 3917066 waaP3 4610108 waaP3 Azoarcus sp. BH72 lipopolysaccharide core biosynthesis protein YP_935072.1 3916293 D 62928 CDS YP_935073.1 119899860 4610109 3917063..3918520 1 NC_008702.1 InterPro: Eukaryotic protein kinase. Might be related to lipopolysaccharide biosynthesis, due to the presence of the gene in a lipopolysaccharide gene cluster.; hypothetical protein 3918520 4610109 azo3571 Azoarcus sp. BH72 hypothetical protein YP_935073.1 3917063 D 62928 CDS YP_935074.1 119899861 4608011 complement(3918579..3920381) 1 NC_008702.1 Lipid A export ATP-binding protein msbA. MsbA (TC 3.A.1.106.1) is involved in lipid A and possibly also glycerophospholipids export. The transmembrane domain (TMD) forms a pore in the inner membrane and the ATP-binding domain (NBD) is responible for energy generation. Similar to TREMBL:Q9HUG8; TREMBL:Q88D92; SWISSPROT:P60752 (39% identity). Pfam (PF00005): ABC transporter. Pfam (PF00664): ABC transporter transmembrane region. TMHMM reporting five transmembrane helices. TC (3.A.1.106): The Lipid Exporter (LipidE) Family.; High confidence in function and specificity; lipid A export ATP-binding/permease 3920381 msbA2 4608011 msbA2 Azoarcus sp. BH72 lipid A export ATP-binding/permease YP_935074.1 3918579 R 62928 CDS YP_935075.1 119899862 4609276 3920557..3921198 1 NC_008702.1 Conserved hypothetical protein, 43% identity(65% similarity) to TrEMBL|Q9HUG7,Hypothetical protein PA4998 [PA4998] [Pseudomonas aeruginosa]. Has PF06293:IPR010440:Lipopolysaccharide kinase (Kdo/WaaP) family;These lipopolysaccharide kinases are related to protein kinases Pkinase. This family includes waaP (rfaP) gene product is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide (LPS) inner core region. It has previously been shown that WaaP is necessary for resistance to hydrophobic and polycationic antimicrobials in E. coli and that it is required for virulence in invasive strains of S. enterica. No Signal Peptide or TMH reported present.; hypothetical protein 3921198 4609276 azo3573 Azoarcus sp. BH72 hypothetical protein YP_935075.1 3920557 D 62928 CDS YP_935076.1 119899863 4608012 complement(3921206..3922345) 1 NC_008702.1 Hypothetical membrane protein. Homology to pspto4986 of P. syringae of 28% (trembl|Q87VF1(SRS)) No domains predicted signal peptide 9 TMHs; hypothetical protein 3922345 4608012 azo3574 Azoarcus sp. BH72 hypothetical protein YP_935076.1 3921206 R 62928 CDS YP_935077.1 119899864 4608013 complement(3922367..3923167) 1 NC_008702.1 Family membership; pyruvyltransferase 3923167 4608013 azo3575 Azoarcus sp. BH72 pyruvyltransferase YP_935077.1 3922367 R 62928 CDS YP_935078.1 119899865 4608014 complement(3923220..3924176) 1 NC_008702.1 hypothetical protein 3924176 4608014 azo3576 Azoarcus sp. BH72 hypothetical protein YP_935078.1 3923220 R 62928 CDS YP_935079.1 119899866 4608015 complement(3924173..3925075) 1 NC_008702.1 Possibly related to cell surface polysaccharide biosynthesis, due to the presence of the gene in a polysaccharide synthesis gene cluster.; hypothetical protein 3925075 4608015 azo3577 Azoarcus sp. BH72 hypothetical protein YP_935079.1 3924173 R 62928 CDS YP_935080.1 119899867 4608016 complement(3925072..3926202) 1 NC_008702.1 Specificity unclear; glycosyltransferase 3926202 4608016 azo3578 Azoarcus sp. BH72 glycosyltransferase YP_935080.1 3925072 R 62928 CDS YP_935081.1 119899868 4608017 complement(3926195..3927979) 1 NC_008702.1 Putative carbamoyltransferase. Homology to nolU of Rhizobium sp. NGR234 of 33% InterPro: Carbamoyltransferase Pfam: Carbamoyltransferase no signal peptide no TMHs; Family membership; putative carbamoyltransferase 3927979 nolU 4608017 nolU Azoarcus sp. BH72 putative carbamoyltransferase YP_935081.1 3926195 R 62928 CDS YP_935082.1 119899869 4609394 complement(3928043..3930106) 1 NC_008702.1 Catalysis of the reactions of the type: acyl-carrier + reactant = acyl-reactant + carrier. Entry name : Q888M0 InterPro : IPR002656; Acyl_transf_3. Identity: 227/677 (33%) Pfam PF01757; Acyl_transf_3; 1. Prediction: Non-secretory protein Signal peptide probability: 0.005(almost Yes) Number of predicted TMHs: 10; Family membership; putative acyltransferase family protein 3930106 4609394 azo3580 Azoarcus sp. BH72 putative acyltransferase family protein YP_935082.1 3928043 R 62928 CDS YP_935083.1 119899870 4608018 complement(3930274..3932139) 1 NC_008702.1 48% Polysac_CapD. Pfam:PF02719; Polysacc_synt_2; 1.; High confidence in function and specificity; putative capsular polysaccharide biosynthesis protein 3932139 capD 4608018 capD Azoarcus sp. BH72 putative capsular polysaccharide biosynthesis protein YP_935083.1 3930274 R 62928 CDS YP_935084.1 119899871 4608413 complement(3932553..3933590) 1 NC_008702.1 Similar to proteins annotated as phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (UDP- MurNAc-pentapeptide phosphotransferase). First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan (By similarity).; Function unclear; cell envelope biogenesis membrane protein 3933590 4608413 azo3582 Azoarcus sp. BH72 cell envelope biogenesis membrane protein YP_935084.1 3932553 R 62928 CDS YP_935085.1 119899872 4608019 complement(3933587..3934570) 1 NC_008702.1 50%; High confidence in function and specificity; putative UDP-glucose 4-epimerase 3934570 wbpV 4608019 wbpV Azoarcus sp. BH72 putative UDP-glucose 4-epimerase YP_935085.1 3933587 R 62928 CDS YP_935086.1 119899873 4610111 complement(3934567..3935748) 1 NC_008702.1 InterPro: Glycosyl transferases group 1; Specificity unclear; glycosyltransferase 3935748 4610111 azo3584 Azoarcus sp. BH72 glycosyltransferase YP_935086.1 3934567 R 62928 CDS YP_935087.1 119899874 4608020 complement(3935717..3936460) 1 NC_008702.1 Putative glycosyl transferase MJ1057 (EC 2.-.-.-). InterPro: Glycosyl transferase family 2; Family membership; glycosyltransferase 3936460 4608020 azo3585 Azoarcus sp. BH72 glycosyltransferase YP_935087.1 3935717 R 62928 CDS YP_935088.1 119899875 4608021 complement(3936457..3937452) 1 NC_008702.1 GDP-L-fucose synthetase (GDP-4-keto-6-deoxy-D-mannose-35-epimerase -4-reductase). Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose involving an epimerase and a reductase reaction.54% ADH_short.IPR001509; Epimerase_Dh. Pfam:PF01370; Epimerase. Non-secretory protein.; High confidence in function and specificity; putative GDP-fucose synthetase 3937452 fcl1 4608021 fcl1 Azoarcus sp. BH72 putative GDP-fucose synthetase YP_935088.1 3936457 R 62928 CDS YP_935089.1 119899876 4608737 complement(3937403..3938488) 1 NC_008702.1 [EC:4.2.1.47] , GDP-mannose 4,6-dehydratase (EC 4.2.1.47) (GDP-D-mannose dehydratase)., GDP-mannose 4,6-dehydratase, NAD dependent epimerase/dehydratase family,gmd: GDP-mannose 4,6-dehydratase; High confidence in function and specificity; GDP-mannose 4,6-dehydratase 3938488 gmd 4608737 gmd Azoarcus sp. BH72 GDP-mannose 4,6-dehydratase YP_935089.1 3937403 R 62928 CDS YP_935090.1 119899877 4608926 complement(3938557..3940620) 1 NC_008702.1 Hypothetical membrane protein. No homology to the data bank. No domains predicted. no signal peptide. 9 TMHs; Conserved hypothetical protein; hypothetical protein 3940620 4608926 azo3588 Azoarcus sp. BH72 hypothetical protein YP_935090.1 3938557 R 62928 CDS YP_935091.1 119899878 4608022 complement(3940575..3941408) 1 NC_008702.1 InterPro: Glycosyl transferase family 2. Slight similarity to dolicholphosphate mannosyltransferases,might therefore transfer a sugar moiety directly to a lipid carrier.; Specificity unclear; glycosyltransferase 3941408 4608022 azo3589 Azoarcus sp. BH72 glycosyltransferase YP_935091.1 3940575 R 62928 CDS YP_935092.1 119899879 4608023 complement(3941422..3941778) 1 NC_008702.1 Low similarity with the EmrE protein (Methyl viologen resistance protein C)(Ethidium resistance protein)in E.coli. Multidrug transporter. Confers resistance to a wide range of toxic compounds by removing them for the cells. The efflux is coupled to an influx of protons. Probably prevents the incorporation of methyl viologen into cells. Involved in ethidium bromide efflux. EMRE_ECOLI:P23895. InterPro:IPR000390; Smr. Pfam: PF00893; Multi_Drug_Res; 1. Signal peptide present. TMHelix: 4.; High confidence in function and specificity; multidrug efflux transporter protein 3941778 emrE 4608023 emrE Azoarcus sp. BH72 multidrug efflux transporter protein YP_935092.1 3941422 R 62928 CDS YP_935093.1 119899880 4608666 complement(3941775..3942605) 1 NC_008702.1 Family membership; sugar or sugar nucleotide oxidoreductase 3942605 4608666 azo3591 Azoarcus sp. BH72 sugar or sugar nucleotide oxidoreductase YP_935093.1 3941775 R 62928 CDS YP_935094.1 119899881 4608024 complement(3942602..3943387) 1 NC_008702.1 Gene located in a gene cluster related to an unidentified cell surface polysaccharide; hypothetical protein 3943387 4608024 azo3592 Azoarcus sp. BH72 hypothetical protein YP_935094.1 3942602 R 62928 CDS YP_935095.1 119899882 4608025 complement(3943384..3944523) 1 NC_008702.1 Hypothetical membrane protein. No good homology of the entire protein with the data bank. No domains predicted. No signal peptide. 1 TMH; hypothetical protein 3944523 4608025 azo3593 Azoarcus sp. BH72 hypothetical protein YP_935095.1 3943384 R 62928 CDS YP_935096.1 119899883 4608026 complement(3944541..3945566) 1 NC_008702.1 Putative glycosyl transferase sll0501 (EC 2.-.-.-).; Specificity unclear; glycosyltransferase 3945566 4608026 azo3594 Azoarcus sp. BH72 glycosyltransferase YP_935096.1 3944541 R 62928 CDS YP_935097.1 119899884 4608027 complement(3945793..3948288) 1 NC_008702.1 hypothetical protein 3948288 4608027 azo3595 Azoarcus sp. BH72 hypothetical protein YP_935097.1 3945793 R 62928 CDS YP_935098.1 119899885 4608028 complement(3948285..3949694) 1 NC_008702.1 44% AAA_ATPase.IPR003439; ABC_transporter. Pfam:PF00005; ABC_tran; 1. ProDom:PD000006; ABC_transporter; 1. SMART:SM00382; AAA; 1.; putative polysaccharide export transport system ATP-binding protein 3949694 wzt 4608028 wzt Azoarcus sp. BH72 putative polysaccharide export transport system ATP-binding protein YP_935098.1 3948285 R 62928 CDS YP_935099.1 119899886 4610121 complement(3949684..3950472) 1 NC_008702.1 51% ABC_transpt2. Pfam:PF01061; ABC2_membrane; 1. TMhelix:6. Non-secretory protein.; Specificity unclear; putative ABC polysaccharide exporter permease 3950472 wzm 4610121 wzm Azoarcus sp. BH72 putative ABC polysaccharide exporter permease YP_935099.1 3949684 R 62928 CDS YP_935100.1 119899887 4610120 complement(3950578..3952557) 1 NC_008702.1 Possibly related to cell surface biosynthesis, ue to the location of he gene in a polysaccharide synthesis gene cluster.; hypothetical protein 3952557 4610120 azo3598 Azoarcus sp. BH72 hypothetical protein YP_935100.1 3950578 R 62928 CDS YP_935101.1 119899888 4608029 complement(3952636..3953631) 1 NC_008702.1 49%; High confidence in function and specificity; putative GDP-fucose synthetase 3953631 fcl2 4608029 fcl2 Azoarcus sp. BH72 putative GDP-fucose synthetase YP_935101.1 3952636 R 62928 CDS YP_935102.1 119899889 4608738 complement(3953917..3956673) 1 NC_008702.1 DNA polymerase I (EC 2.7.7.7) (POL I). IN ADDITION TO POLYMERASE ACTIVITY THIS DNA POLYMERASE EXHIBITS 3 TO 5 AND 5 TO 3 EXONUCLEASE ACTIVITY. IT IS ABLE TO UTILIZE NICKED CIRCULAR DUPLEX DNA AS A TEMPLATE AND CAN UNWIND THE PARENTAL DNA STRAND FROM ITS TEMPLATE. InterPro: Replicative DNA polymerase; High confidence in function and specificity; DNA polymerase I 3956673 polA 4608738 polA Azoarcus sp. BH72 DNA polymerase I YP_935102.1 3953917 R 62928 CDS YP_935103.1 119899890 4609596 3956755..3957477 1 NC_008702.1 Hypothetical protein PA4923. TREMBL:Q82UL5: 68% identity, 82% similarity InterPro:IPR005269; Cons_hypoth730. Pfam:PF03641; Lysine_decarbox; 1. TIGRFAMs:TIGR00730; Cons_hypoth730 bcl-2: Apoptosis regulator Non-secretory protein with signal peptide probability 0(Signal P predicted9 Absence of transmembrane helices (TMHHH predicted); Function unclear; hypothetical protein 3957477 4609596 azo3601 Azoarcus sp. BH72 hypothetical protein YP_935103.1 3956755 D 62928 CDS YP_935104.1 119899891 4608030 3957597..3957920 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to CV0777 of C.violaceum of 42% (trembl|Q7NZZ2(SRS)). No domains predicted. Signal peptide present. No TMH reported.; Conserved hypothetical protein; hypothetical protein 3957920 4608030 azo3602 Azoarcus sp. BH72 hypothetical protein YP_935104.1 3957597 D 62928 CDS YP_935105.1 119899892 4608031 3957899..3958459 1 NC_008702.1 hypothetical protein 3958459 4608031 azo3603 Azoarcus sp. BH72 hypothetical protein YP_935105.1 3957899 D 62928 CDS YP_935106.1 119899893 4608032 3958480..3959433 1 NC_008702.1 catalyzes the formation of O-phospho-L-homoserine from L-homoserine; homoserine kinase 3959433 thrB 4608032 thrB Azoarcus sp. BH72 homoserine kinase YP_935106.1 3958480 D 62928 CDS YP_935107.1 119899894 4609943 3959528..3960298 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to cv0192 of C. violaceum of 32% (trembl|Q7P1M1(SRS)). No domains predicted. No signal peptide. 6 TMHs.; Conserved hypothetical protein; hypothetical protein 3960298 4609943 azo3605 Azoarcus sp. BH72 hypothetical protein YP_935107.1 3959528 D 62928 CDS YP_935108.1 119899895 4608033 3960306..3961076 1 NC_008702.1 Conserved hypothetical protein. Homology to ne1472 of N. europaea of 33% (trembl|Q82UL2(SRS)). No domains predicted. No signal peptide. 6 TMHs.; hypothetical protein 3961076 4608033 azo3606 Azoarcus sp. BH72 hypothetical protein YP_935108.1 3960306 D 62928 CDS YP_935109.1 119899896 4608034 3961057..3961740 1 NC_008702.1 Probable nicotinate-nucleotide adenylyltransferase (Deamido-NAD(+) pyrophosphorylase) (Deamido-NAD(+) diphosphorylase) (Nicotinate mononucleotide adenylyltransferase) (NaMN adenylyltransferase). Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD) (By similarity). InterPro: Cytidylyltransferase; High confidence in function and specificity; nicotinate-nucleotide adenylyltransferase 3961740 nadD 4608034 nadD Azoarcus sp. BH72 nicotinate-nucleotide adenylyltransferase YP_935109.1 3961057 D 62928 CDS YP_935110.1 119899897 4609305 3961760..3962125 1 NC_008702.1 Conserved hypothetical protein. Homology to NE0358 of Nitrosomonas europaea of 55% (trembl:Q82XC6). Pfam:Domain of unknown function DUF143. This domain has no known function nor do any of the proteins that possess it. The aligned region is approximately 100 amino acids long. Tigrfam: TIGR00090 iojap-related protein. This model describes a strictly bacterial family of proteins related to iojap from plants. The gene iojap is a pattern-striping gene in maize, reflecting a chloroplast development defect in some cells. Maize has two RNA polymerases in plastids,but the plastid-encoded one; hypothetical protein 3962125 4609305 azo3608 Azoarcus sp. BH72 hypothetical protein YP_935110.1 3961760 D 62928 CDS YP_935111.1 119899898 4608035 3962122..3962592 1 NC_008702.1 SPOUT methyltransferase family protein; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA; rRNA large subunit methyltransferase 3962592 4608035 azo3609 Azoarcus sp. BH72 rRNA large subunit methyltransferase YP_935111.1 3962122 D 62928 CDS YP_935112.1 119899899 4608036 3962595..3963128 1 NC_008702.1 Probable peptide deformylase (EC 3.5.1.88). Homology to def of E. coli of 42% (sprot|DEF_ECOLI(SRS)). Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions Interpro: Formylmethionine deformylase (IPR000181) Pfam: Polypeptide deformylase Tigrfam: pept_deformyl: polypeptide deformylase no signal peptide no TMHs; High confidence in function and specificity; peptide deformylase 3963128 def2 4608036 def2 Azoarcus sp. BH72 peptide deformylase YP_935112.1 3962595 D 62928 CDS YP_935113.1 119899900 4608617 3963195..3963815 1 NC_008702.1 Homology to Maf-like proteins; Family membership; Maf-like protein 3963815 4608617 azo3611 Azoarcus sp. BH72 Maf-like protein YP_935113.1 3963195 D 62928 CDS YP_935114.1 119899901 4608037 3963871..3965382 1 NC_008702.1 Ribonuclease G (EC 3.1.4.-) (RNase G) (Cytoplasmic axial filament protein). INVOLVED IN PROCESSING OF THE 5END OF 16S RRNA. COULD BE INVOLVED IN CHROMOSOME SEGREGATION AND CELL DIVISION. IT MAY BE ONE OF THE COMPONENTS OF THE CYTOPLASMIC AXIAL FILAMENTS BUNDLES OR MERELY REGULATE THE FORMATION OF THIS STRUCTURE.; Family membership; putative ribonuclease 3965382 cafA 4608037 cafA Azoarcus sp. BH72 putative ribonuclease YP_935114.1 3963871 D 62928 CDS YP_935115.1 119899902 4608410 3965391..3966347 1 NC_008702.1 Probable 14-dihydroxy-2-naphthoate octaprenyltransferase (EC 2.5.1.-) (DHNA-octaprenyltransferase). CONVERSION OF 1,4-DIHYDROXY-2-NAPHTHOATE (DHNA) TO DIMETHYLMENAQUINONE (DMK). ATTACHES OCTAPRENYLPYROPHOSPHATE A MEMBRANE-BOUND 40-CARBON SIDE CHAIN TO DHNA. THE CONVERSION OF DHNA TO DMK PROCEEDS IN THREE STAGES: THE REMOVAL OF THE CARBOXYL GROUP OF DHNA AS CO2 THE ATTACHMENT OF THE ISOPRENOID SIDE CHAIN AND A QUINOL-TO-QUINONE OXIDATION WHICH IS THOUGHT TO BE SPONTANEOUS (BY SIMILARITY). TIGRFAM: menA: 14-dihydroxy-2-naphthoate octaprenyltransferase; Specificity unclear; 1,4-dihydroxy-2-naphthoate octaprenyltransferase 3966347 menA 4608410 menA Azoarcus sp. BH72 1,4-dihydroxy-2-naphthoate octaprenyltransferase YP_935115.1 3965391 D 62928 CDS YP_935116.1 119899903 4609207 complement(3966362..3966727) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PA0359 of Pseudomonas aeruginosa of 35% (trembl|Q9I6D5(SRS)). No domains predicted. signal peptide. 1 TMHs; Conserved hypothetical protein; hypothetical protein 3966727 4609207 azo3614 Azoarcus sp. BH72 hypothetical protein YP_935116.1 3966362 R 62928 CDS YP_935117.1 119899904 4608038 3966875..3967591 1 NC_008702.1 Transcriptional regulatory protein, Trans_reg_C. IPR001789 Response_reg. Pfam: PF00486; trans_reg_C. PF00072 response_reg. SMART: SM00448 REC.; Specificity unclear; DNA-binding response regulator 3967591 ompR3 4608038 ompR3 Azoarcus sp. BH72 DNA-binding response regulator YP_935117.1 3966875 D 62928 CDS YP_935118.1 119899905 4609448 3967591..3968691 1 NC_008702.1 Putative sensor histidine kinase, ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR005467; His_kinase. IPR003661; His_kinA_N. IPR009082; His_kin_homodim. IPR003660; HAMP. Pfam: PF02518; HATPase_c. PF00512; HisKA. PF00672; HAMP. SMART: SM00387; HATPase_c. SM00388; HisKA. SM00304; HAMP. TMHMM reporting 2 transmembrane helices.; Specificity unclear; putative sensor histidine kinase 3968691 envZ3 4609448 envZ3 Azoarcus sp. BH72 putative sensor histidine kinase YP_935118.1 3967591 D 62928 CDS YP_935119.1 119899906 4608673 3968809..3969477 1 NC_008702.1 Putative calcium binding protein,40% similarity to TrEMBL;O22845. Has 3 EFh|EF-hand, calcium binding motif;(SMART|SM00054):Many calcium-binding proteins belong to the same evolutionary family and share a type of calcium-binding domain known as the EF-hand. This type of domain consists of a twelve residue loop flanked on both side by a twelve residue alpha-helical domain. In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. The six residues involved in the binding are in positions 1, 3, 5, 7, 9 and 12; these residues are denoted by X, Y, Z, -Y, -X and -Z. The invariant Glu or Asp at position 12 provides two oxygens for liganding Ca (bidentate ligand). NO Signal peptide or TMh reported present.; Family membership; putative calcium binding protein 3969477 4608673 azo3617 Azoarcus sp. BH72 putative calcium binding protein YP_935119.1 3968809 D 62928 CDS YP_935120.1 119899907 4608039 complement(3969509..3970477) 1 NC_008702.1 Glycine cleavage system transcriptional activator (Gcv operon activator). Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation og gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter. Similar to SWISSPROT: sprot|GCVA_ECOLI (39% Escherichia coli, glycine cleavage system transcriptional activator (gcv operon activator)) InterPro: IPR000847 HTH_LysR. IPR009058 Winged helix DNA-binding. Pfam: PF00126 Bacterial regulatory helix-turn-helix protein,lysR family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; glycine cleavage system transcriptional activator 3970477 gcvA 4608039 gcvA Azoarcus sp. BH72 glycine cleavage system transcriptional activator YP_935120.1 3969509 R 62928 CDS YP_935121.1 119899908 4608869 3970592..3970765 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 3970765 4608869 azo3619 Azoarcus sp. BH72 hypothetical protein YP_935121.1 3970592 D 62928 CDS YP_935122.1 119899909 4608040 complement(3970934..3971842) 1 NC_008702.1 Putative adenylate/ guanylate cyclase,; Function unclear; putative adenylate/guanylate cyclase 3971842 4608040 azo3620 Azoarcus sp. BH72 putative adenylate/guanylate cyclase YP_935122.1 3970934 R 62928 CDS YP_935123.1 119899910 4608041 complement(3971984..3972556) 1 NC_008702.1 DNA-3-methyladenine glycosylase I (EC 3.2.2.20) (3-methyladenine-DNA glycosylase I constitutive) (TAG I) . HYDROLYSIS OF THE DEOXYRIBOSE N-GLYCOSIDIC BOND TO EXCISE 3-METHYLADENINE FROM THE DAMAGED DNA POLYMER FORMED BY ALKYLATION LESIONS. InterPro: DNA-3-methyladenine glycosylase I; High confidence in function and specificity; DNA-3-methyladenine glycosidase I 3972556 tag 4608041 tag Azoarcus sp. BH72 DNA-3-methyladenine glycosidase I YP_935123.1 3971984 R 62928 CDS YP_935124.1 119899911 4609912 complement(3972582..3973490) 1 NC_008702.1 Probable transcriptional regulator, LysR family,; Family membership; LysR family transcriptional regulator 3973490 ptxR 4609912 ptxR Azoarcus sp. BH72 LysR family transcriptional regulator YP_935124.1 3972582 R 62928 CDS YP_935125.1 119899912 4609653 3973580..3974692 1 NC_008702.1 Alcohol dehydrogenase class III (Formaldehyde dehydrogenase (glutathione)). Homology to fdh of E. coli of 72% (trembl|Q59399) CLASS-III ADH IS REMARKABLY INEFFECTIVE IN OXIDIZING ETHANOL BUT IT READILY CATALYZES THE OXIDATION OF LONG-CHAIN PRIMARY ALOCHOLS AND THE OXIDATION OF S-(HYDROXYMETHYL) GLUTATHIONE (BY SIMILARITY). InterPro: Zinc-containing alcohol dehydrogenase superfamily (IPR002085) Zinc-containing alcohol dehydrogenase (IPR002328) Pfam: zinc-binding dehydrogenase Tigrfam: tdh: L-threonine 3-dehydrogenase. no signal peptide. 2 TMHs; High confidence in function and specificity; alcohol dehydrogenase classIII 3974692 adhC 4609653 adhC Azoarcus sp. BH72 alcohol dehydrogenase classIII YP_935125.1 3973580 D 62928 CDS YP_935126.1 119899913 4608280 3974733..3975587 1 NC_008702.1 TREMBL:Q88ME4: 72% identity,83% similarity Esterase D (EC 3.1.1.1). This family contains several seemingly unrelated proteins, including human esterase D; mycobacterial antigen 85, which is responsible for the high affinity of mycobacteria to fibronectin; Corynebacterium glutamicum major secreted protein PS1; and hypothetical proteins from Escherichia coli, yeast,mycobacteria and Haemophilus influenzae. InterPro:IPR000801; Esterase_put. IPR000379; Ser_estrs. Pfam: PF00756; Esterase; InterPro: Putative esterase Absence of signal peptide and transmembrane helices; Specificity unclear; esterase 3975587 4608280 azo3624 Azoarcus sp. BH72 esterase YP_935126.1 3974733 D 62928 CDS YP_935127.1 119899914 4608042 complement(3975594..3976508) 1 NC_008702.1 Hypothetical membrane protein. no homology to the data bank no doamains predicted no signal peptide 1 TMH; hypothetical protein 3976508 4608042 azo3625 Azoarcus sp. BH72 hypothetical protein YP_935127.1 3975594 R 62928 CDS YP_935128.1 119899915 4608043 3976698..3977960 1 NC_008702.1 Conserved hypothetical chaperon protein: Homology to cv2395 of C. violaceum of 62% (trembl|Q7NVE8). InterPro: Heat shock protein hsp70 (IPR001023). Pfam: Hsp70 protein. no signal peptide. no TMHs; Family membership; chaperon protein 3977960 4608043 azo3626 Azoarcus sp. BH72 chaperon protein YP_935128.1 3976698 D 62928 CDS YP_935129.1 119899916 4608044 complement(3978018..3978974) 1 NC_008702.1 Conserved hypothetical SLT domain protein. Homology to NE2226 of N.europaea of 42% (trembl|Q82SS9(SRS). Pfam: Transglycosylase SLT domain. Bacterial lytic transglycosylases degrade murein via cleavage of the beta-1,4- glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine, with the concomitant formation of a 1,6-anhydrobond in the muramic acid residue. Escherichia coli has at least three different lytic transglycosylases: two soluble isozymes of 65 Kd and 35 Kd and a membrane-bound enzyme of 38 Kd. The sequence of the 65 Kd enzyme (gene slt) has been determined. A domain of about 90 residues located near the C-terminal section of slt was recently shown to be present in a number of other prokaryotic and phage proteins. This SLT domain shared by these proteins is involved in catalytic activity. The most conserved part of this domain contains the contains two conserved serines and a glutamate which form part of this active site signature. no TMHs. signal peptide; Conserved hypothetical protein; hypothetical protein 3978974 4608044 azo3627 Azoarcus sp. BH72 hypothetical protein YP_935129.1 3978018 R 62928 CDS YP_935130.1 119899917 4608045 3979292..3979768 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology of the entire protein to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 3979768 4608045 azo3628 Azoarcus sp. BH72 hypothetical protein YP_935130.1 3979292 D 62928 CDS YP_935131.1 119899918 4608046 complement(3979900..3980787) 1 NC_008702.1 Transcriptional regulator, LysR family,; High confidence in function and specificity; LysR family transcriptional regulator 3980787 crgA 4608046 crgA Azoarcus sp. BH72 LysR family transcriptional regulator YP_935131.1 3979900 R 62928 CDS YP_935132.1 119899919 4608533 3980888..3982096 1 NC_008702.1 Sugar efflux transporter protein, involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports L-arabinose and to a lesser extent IPTG. Seems to contribute to the control of the arabinose regulon. 26% MFS.IPR005828; Sub_transporter. Belongs to major facilitator superfamily. SotB (TC 2.A.1.2) family. Signal peptide:present. TMHelix:12.; Specificity unclear; putative transmembrane efflux protein 3982096 ydeA 4608533 ydeA Azoarcus sp. BH72 putative transmembrane efflux protein YP_935132.1 3980888 D 62928 CDS YP_935133.1 119899920 4610164 3982119..3982925 1 NC_008702.1 25-diketo-D-gluconic acid reductase B (EC 1.1.1.274) (25-DKG reductase B) (25-DKGR B) (25DKGR-B) (AKR5D). TREMBL:Q8Y090: 67% identity, 78% similarity. Catalyzes the reduction of 25-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) (By similarity). subunit:monomer (by similarity). subcellular location:cytoplasmic (by similarity). 2-keto-l-gulonic acid is a key intermediate in the production of l-ascorbic acid (vitamin c). belongs to the aldo/keto reductase family InterPro:IPR001395; Aldo/ket_red. Pfam:PF00248; aldo_ket_red; 1. PRINTS PR00069; ALDKETRDTAS ribH: riboflavin synthase beta subunit No signal peptide (non secretory protein) predicted by Signal P and no transmembrane helices (predicted by TMHMM).; High confidence in function and specificity; 2,5-diketo-D-gluconate reductase B 3982925 yafB 4610164 yafB Azoarcus sp. BH72 2,5-diketo-D-gluconate reductase B YP_935133.1 3982119 D 62928 CDS YP_935134.1 119899921 4610138 3982978..3984780 1 NC_008702.1 ATP-binding cassette (ABC) transporters form a large family of proteins responsible for translocation of a variety of compounds across biological membranes. They are composed of two transmembrane domains responsible for binding and transport and two nucleotide-binding domains responsible for coupling the energy of ATP hydrolysis to conformational changes in the TMDs. Similar to TREMBL:Q89UA9 (44% identity); TREMBL:Q92M72 (44% identity); SWISSPROT:Q9JI39 (32% identity). InterPro (IPR003439): ABC transporter. InterPro (IPR003593): AAA ATPase. InterPro (IPR001687): ATP/GTP-binding site motif A (P-loop). InterPro (IPR001140): ABC transportertransmembrane region. Pfam (PF00664): ABC transporter transmembrane region. Pfam (PF00005): ABC transporter. TMHMM reporting six transmembrane helices. TC (3.A.1): The ATP-binding Cassette (ABC) Superfamily.; High confidence in function and specificity; ABC transporter permease and ATP-binding protein 3984780 4610138 azo3632 Azoarcus sp. BH72 ABC transporter permease and ATP-binding protein YP_935134.1 3982978 D 62928 CDS YP_935135.1 119899922 4608047 3985096..3985944 1 NC_008702.1 Hypothetical secreted protein. No significant homology over the entier protein. signal peptide no TMHs; hypothetical protein 3985944 4608047 azo3633 Azoarcus sp. BH72 hypothetical protein YP_935135.1 3985096 D 62928 CDS YP_935136.1 119899923 4608048 complement(3986061..3988160) 1 NC_008702.1 Probable D-(-)-3-hydroxybutyrate oligomer hydrolase. Homology to a orf of Ralstonia pickettii of 48% (gi|2154711|dbj|BAA20331.1|(NCBI ENTREZ)). Catalysis of the reaction: (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate. No doamis predicted. Signal peptide. TMHs in signal peptide.,; Family membership; D-(-)-3-hydroxybutyrate oligomer hydrolase 3988160 4608048 azo3634 Azoarcus sp. BH72 D-(-)-3-hydroxybutyrate oligomer hydrolase YP_935136.1 3986061 R 62928 CDS YP_935137.1 119899924 4608049 3988511..3989053 1 NC_008702.1 Conserved hypothetical acetyltransferase. Homology to cv2337 of C. violaceum of 43% (AAQ60009). InterPro: GCN5-related N-acetyltransferase (IPR000182). Pfam: Acetyltransferase (GNAT) family. no signal peptide. no TMHs; Conserved hypothetical protein; acetyltransferase 3989053 4608049 azo3635 Azoarcus sp. BH72 acetyltransferase YP_935137.1 3988511 D 62928 CDS YP_935138.1 119899925 4608050 complement(3989120..3990949) 1 NC_008702.1 Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (Hexosephosphate aminotransferase) (D-fructose-6- phosphate amidotransferase) (GFAT) (L-glutamine-D-fructose-6-phosphate amidotransferase) (Glucosamine-6-phosphate synthase). Catalyzes the first step in hexosamine metabolism converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source (By similarity). InterPro: Glutamine amidotransferase class-II TIGR00274: glucokinase regulator-rela; High confidence in function and specificity; glucosamine-fructose-6-phosphate aminotransferase 3990949 glmS 4608050 glmS Azoarcus sp. BH72 glucosamine-fructose-6-phosphate aminotransferase YP_935138.1 3989120 R 62928 CDS YP_935139.1 119899926 4608892 complement(3990992..3992350) 1 NC_008702.1 Bifunctional glmU protein [Includes: UDP-N-acetylglucosamine pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine-1-phosphate uridyltransferase); Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.57)]. Bifunctional enzyme responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and the synthesis of UDP-GlcNAc. InterPro: ADP-glucose pyrophosphorylase ispD: 4-diphosphocytidyl-2C-methyl-D-e; High confidence in function and specificity; UDP-N-acetylglucosamine pyrophosphorylase/diamine N-acetyltransferase 3992350 glmU 4608892 glmU Azoarcus sp. BH72 UDP-N-acetylglucosamine pyrophosphorylase/diamine N-acetyltransferase YP_935139.1 3990992 R 62928 CDS YP_935140.1 119899927 4608893 3992536..3993945 1 NC_008702.1 Conserved hypothetical phosphate acetyltransferase. Homology to pta of R. palustris of 63% (tremblnew|CAE30007). InterPro: Phosphate acetyl/butaryl transferase (IPR002505); MaoC-like dehydrogenase domain (IPR002539). Pfam: MaoC like domain; Phosphate acetyl/butaryl transferase. no signal peptide. no TMHs; Conserved hypothetical protein; bifunctional enoyl-CoA hydratase/phosphate acetyltransferase 3993945 pta 4608893 pta Azoarcus sp. BH72 bifunctional enoyl-CoA hydratase/phosphate acetyltransferase YP_935140.1 3992536 D 62928 CDS YP_935141.1 119899928 4609645 3993942..3995129 1 NC_008702.1 probable acetate kinase. Homology to ackA of s. meliloti of 46% (sprot|ACK2_RHIME) Acetate kinase is involved in the activation of acetate to acetyl CoA and in the secretion of acetate. It catalyzes the reaction ATP + acetate = ADP + acetyl phosphate. InterPro: Acetate kinase (IPR004372); acetate and butyrate kinase (IPR000890) Pfam: Acetokinase family Tigrfam: ackA: acetate kinase no singal peptide no TMHs; High confidence in function and specificity; acetate kinase 3995129 ackA 4609645 ackA Azoarcus sp. BH72 acetate kinase YP_935141.1 3993942 D 62928 CDS YP_935142.1 119899929 4608262 complement(3995147..3995914) 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q9A2S0: 40% identity InterPro:IPR002781; DUF81. Pfam:PF01925; DUF81. Non secretory protein (signal peptide probablity:0.035) and presence of 7 transmembrane helices (TMHMM predicted).; Conserved hypothetical protein; hypothetical protein 3995914 4608262 azo3640 Azoarcus sp. BH72 hypothetical protein YP_935142.1 3995147 R 62928 CDS YP_935143.1 119899930 4608051 complement(3996223..3997689) 1 NC_008702.1 glutamate synthase is composed of subunits alpha and beta; beta subunit is a flavin adenine dinucleotide-NADPH dependent oxidoreductase; provides electrons to the alpha subunit, which binds L-glutamine and 2-oxoglutarate and forms L-glutamate; glutamate synthase subunit beta 3997689 gltD 4608051 gltD Azoarcus sp. BH72 glutamate synthase subunit beta YP_935143.1 3996223 R 62928 CDS YP_935144.1 119899931 4608916 complement(3997694..4002367) 1 NC_008702.1 Ferredoxin-dependent glutamate synthase,; Specificity unclear; ferredoxin-dependent glutamate synthase 4002367 gltB 4608916 gltB Azoarcus sp. BH72 ferredoxin-dependent glutamate synthase YP_935144.1 3997694 R 62928 CDS YP_935145.1 119899932 4608915 complement(4002629..4003768) 1 NC_008702.1 dGTPase family type 2 subfamily; presumably hydrolyzes dGTP to deoxyguanosine and triphosphate; deoxyguanosinetriphosphate triphosphohydrolase-like protein 4003768 4608915 azo3643 Azoarcus sp. BH72 deoxyguanosinetriphosphate triphosphohydrolase-like protein YP_935145.1 4002629 R 62928 CDS YP_935146.1 119899933 4608052 complement(4003759..4004841) 1 NC_008702.1 catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis; 3-dehydroquinate synthase 4004841 aroB 4608052 aroB Azoarcus sp. BH72 3-dehydroquinate synthase YP_935146.1 4003759 R 62928 CDS YP_935147.1 119899934 4608343 complement(4004829..4005365) 1 NC_008702.1 catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase 4005365 aroK 4608343 aroK Azoarcus sp. BH72 shikimate kinase YP_935147.1 4004829 R 62928 CDS YP_935148.1 119899935 4608348 complement(4005442..4007565) 1 NC_008702.1 Type 4 fimbrial biogenesis protein PilQ, similarity to sprot|PILQ_PSEAE (41%) and trembl|Q8XV60 (45%). PilQ is essential for the biogenesis of type IV pili. Its precise function is unknown, but it has been suggested that it may act as a pilus channel in the final stages of pilus assembly. InterPro (PF00263): General (type II) secretion pathway (GSP) D protein SignalP reporting signal peptide.; Specificity unclear; type 4 fimbrial biogenesis protein 4007565 pilQ 4608348 pilQ Azoarcus sp. BH72 type 4 fimbrial biogenesis protein YP_935148.1 4005442 R 62928 CDS YP_935149.1 119899936 4609570 complement(4007562..4008077) 1 NC_008702.1 Type 4 fimbrial biogenesis protein, PilP. PilP is essential for the biogenesis of type IV pili. Similar to trembl|Q7NZU1(37%). SignalP reporting Signal Peptide.; Family membership; putative type 4 fimbrial biogenesis protein 4008077 pilP 4609570 pilP Azoarcus sp. BH72 putative type 4 fimbrial biogenesis protein YP_935149.1 4007562 R 62928 CDS YP_935150.1 119899937 4609569 complement(4008074..4008748) 1 NC_008702.1 Type 4 fimbrial biogenesis protein PilO, similarity to pir|S77728 (40%). PilO proteins are involved in the assembly of pilin. TMHMM reporting one TMH.; Specificity unclear; type 4 fimbrial biogenesis protein 4008748 pilO 4609569 pilO Azoarcus sp. BH72 type 4 fimbrial biogenesis protein YP_935150.1 4008074 R 62928 CDS YP_935151.1 119899938 4609568 complement(4008745..4009344) 1 NC_008702.1 Type 4 fimbrial biogenesis protein PilN, similarity to trembl|Q82SK2(51%)and to pir|S77727(42%). In P. aeruginosa PilN was found to be required for fimbrial biogenesis by complementation studies using twitching motility and sensitivity to fimbrial-specific phage as indicators of the presence of functional fimbriae. SignalP reporting signal peptide.; Specificity unclear; type 4 fimbrial biogenesis protein 4009344 pilN 4609568 pilN Azoarcus sp. BH72 type 4 fimbrial biogenesis protein YP_935151.1 4008745 R 62928 CDS YP_935152.1 119899939 4609567 complement(4009341..4010417) 1 NC_008702.1 Type 4 fimbrial biogenesis protein PilM, similarity to trembl|Q82SK1 (56%)and to pir|S77726 (47%). Four genes,pilM-P, encode the necessary proteins and pilM is required for the assembly of the type IV fimbriae in Pseudomonas aeruginosa.; Specificity unclear; type 4 fimbrial biogenesis protein 4010417 pilM 4609567 pilM Azoarcus sp. BH72 type 4 fimbrial biogenesis protein YP_935152.1 4009341 R 62928 CDS YP_935153.1 119899940 4609566 4010609..4012981 1 NC_008702.1 Probable penicillin-binding protein 1A (PBP-1a) (PBP1a) [Includes: Penicillin- insensitive transglycosylase (EC 2.4.2.-) (Peptidoglycan TGase); Penicillin-sensitive transpeptidase (EC 3.4.-.-) (DD-transpeptidase)]. Homology to mrcA of N. meningitides of 47% (sprot|PBPA_NEIMA(SRS)) Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity). InterPro: Penicillin binding protein transpeptidase domain (IPR001460); Glycosyltransferase family 51 (IPR001264) Pfam: Transglycosylase; Penicillin binding protein transpeptidase singal peptide no TMHs HTH predicted; High confidence in function and specificity; penicillin-binding protein 1A 4012981 mrcA 4609566 mrcA Azoarcus sp. BH72 penicillin-binding protein 1A YP_935153.1 4010609 D 62928 CDS YP_935154.1 119899941 4609271 complement(4012971..4013348) 1 NC_008702.1 CyaY protein that belongs to the frataxin family.Probably involved in iron metabolism. 33% similarity to CyaY protein from E.coli. SWISSPROT:CYAY_YERPE:P46356 InterPro:IPR002908; Frataxin_like. Pfam:PF01491; Frataxin_Cyay; 1.; High confidence in function and specificity; putative CyaY protein 4013348 cyaY 4609271 cyaY Azoarcus sp. BH72 putative CyaY protein YP_935154.1 4012971 R 62928 CDS YP_935155.1 119899942 4608547 4013347..4013517 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ypo3846 of Y. pestis of 42% (trembl|Q8ZAF7). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 4013517 4608547 azo3653 Azoarcus sp. BH72 hypothetical protein YP_935155.1 4013347 D 62928 CDS YP_935156.1 119899943 4608053 4013514..4014788 1 NC_008702.1 Diaminopimelate decarboxylase, LysA. Similar to sprot|DCDA_PSEAE (64%). Pyridoxal-dependent decarboxylases that act on ornithine-, lysine-, arginine- and related substrates can be classified into different families on the basis of sequence similarity. Prokaryotic diaminopimelic acid decarboxylase (DAPDC) catalyses the conversion of diaminopimelic acid into lysine, the final step of lysine biosynthesis. InterPro(PR01181): Diaminopimelate decarboxylase InterPro(PF00278): Orn/DAP/Arg decarboxylases family 2; High confidence in function and specificity; LysA2 protein 4014788 lysA2 4608053 lysA2 Azoarcus sp. BH72 LysA2 protein YP_935156.1 4013514 D 62928 CDS YP_935157.1 119899944 4609185 complement(4014811..4015158) 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA2272 of Azoarcus sp. EbN1 of 62% (gnl|keqq|eba:ebA2272(KEGG)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4015158 4609185 azo3655 Azoarcus sp. BH72 hypothetical protein YP_935157.1 4014811 R 62928 CDS YP_935158.1 119899945 4608054 4015219..4016685 1 NC_008702.1 Penicillin-binding protein 4 precursor (PBP-4) [Includes: D-alanyl-D- alanine carboxypeptidase (EC 3.4.16.4) (DD-peptidase) (DD- carboxypeptidase); D-alanyl-D-alanine-endopeptidase (DD-endopeptidase)]. Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction. PBP4: D-alanyl-D-alanine carboxypeptid; High confidence in function and specificity; serine-type D-Ala-D-Ala carboxypeptidase 4016685 dacB 4608054 dacB Azoarcus sp. BH72 serine-type D-Ala-D-Ala carboxypeptidase YP_935158.1 4015219 D 62928 CDS YP_935159.1 119899946 4608576 complement(4016702..4017118) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to bb4211 of B. bronchiseptica of 37% (trembl|Q7WFQ9(SRS)). No domains predicted. No signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 4017118 4608576 azo3657 Azoarcus sp. BH72 hypothetical protein YP_935159.1 4016702 R 62928 CDS YP_935160.1 119899947 4608055 complement(4017168..4018658) 1 NC_008702.1 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-) (Polyprenyl p-hydroxybenzoate decarboxylase). CATALYZES THE DECARBOXYLATION OF 3-OCTAPRENYL-4-HYDROXY BENZOATE TO 2-OCTAPRENYLPHENOL (BY SIMILARITY).; High confidence in function and specificity; 3-polyprenyl-4-hydroxybenzoate decarboxylase 4018658 yigC 4608055 yigC Azoarcus sp. BH72 3-polyprenyl-4-hydroxybenzoate decarboxylase YP_935160.1 4017168 R 62928 CDS YP_935161.1 119899948 4610185 complement(4018668..4019480) 1 NC_008702.1 type 2 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase; orotidine 5'-phosphate decarboxylase 4019480 pyrF 4610185 pyrF Azoarcus sp. BH72 orotidine 5'-phosphate decarboxylase YP_935161.1 4018668 R 62928 CDS YP_935162.1 119899949 4609672 complement(4019543..4020316) 1 NC_008702.1 Modulates the activities of several enzymes which are inactive in their acetylated form; NAD-dependent deacetylase 4020316 npd2 4609672 npd2 Azoarcus sp. BH72 NAD-dependent deacetylase YP_935162.1 4019543 R 62928 CDS YP_935163.1 119899950 4609409 complement(4020405..4022477) 1 NC_008702.1 Conserved hypothetical molybdopterin oxidoreductase. Homology to rsc2859 of R. solanacearum of 60% (trembl|Q8XVH2). Tigrfam: bisC_fam: molybdopterin guanine dinucleotide-containing S/N-oxidoreductases. Pfam: Molybdopterin oxidoreductase; Molydopterin dinucleotide binding domain. Helixturnhelix motif. no signal sequenz. no TMHs; Conserved hypothetical protein; molybdopterin oxidoreductase 4022477 4609409 azo3661 Azoarcus sp. BH72 molybdopterin oxidoreductase YP_935163.1 4020405 R 62928 CDS YP_935164.1 119899951 4608056 complement(4022532..4023209) 1 NC_008702.1 Conserved hypothetical secreted proteinn. Homology to ebA3326 Azoarcus sp. EbN1 of 47% (gnl|keqq|eba:ebA3326(KEGG)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 4023209 4608056 azo3662 Azoarcus sp. BH72 hypothetical protein YP_935164.1 4022532 R 62928 CDS YP_935165.1 119899952 4608057 complement(4023187..4023855) 1 NC_008702.1 involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate; orotate phosphoribosyltransferase 4023855 pyrE 4608057 pyrE Azoarcus sp. BH72 orotate phosphoribosyltransferase YP_935165.1 4023187 R 62928 CDS YP_935166.1 119899953 4609671 4023919..4024701 1 NC_008702.1 Exodeoxyribonuclease (EC 3.1.11.2). InterPro: AP endonucleases family 1; High confidence in function and specificity; exodeoxyribonuclease III 4024701 xthA2 4609671 xthA2 Azoarcus sp. BH72 exodeoxyribonuclease III YP_935166.1 4023919 D 62928 CDS YP_935167.1 119899954 4610131 4024848..4027106 1 NC_008702.1 Conserved hypothetical signaling protein. Homology to IL2267 of Idiomarina loihiensis of 35% (gnl|keqq|ilo:IL2267(KEGG)). InterPro: IPR001633 EAL. IPR000160 GGDEF. Pfam: PF00563 EAL. PF00990 GGDEF. SMART: SM00267 DUF1. SM00052 DUF2. TIGRFAM:TIGR00254 GGDEF. Signaling protein ykoW. Probable signaling protein whose physiological role is not yet known. no signal peptide. No TMHs; Conserved hypothetical protein; putative signaling protein 4027106 4610131 azo3665 Azoarcus sp. BH72 putative signaling protein YP_935167.1 4024848 D 62928 CDS YP_935168.1 119899955 4608058 4027103..4028941 1 NC_008702.1 Putative sensor histidine kinase, low similarity to SWISSPROT: sprot|KINB_PSEAE (13% Pseudomonas aeruginosa,alginate biosynthesis sensor protein KinB) / TREMBL: trembl|Q50853 (13% Myxococcus xanthus, AsgA) InterPro: IPR004358 Bact_sens_pr_C. IPR003661 His_kinA_N. IPR005467 His_kinase. Pfam: PF02518 HATPase_c. PF00512 HisKA. SMART: SM00387 HATPase_c. SM00388 HisKA. TMHMM reporting 1 transmembrane helices.; Family membership; putative sensor histidine kinase 4028941 4608058 azo3666 Azoarcus sp. BH72 putative sensor histidine kinase YP_935168.1 4027103 D 62928 CDS YP_935169.1 119899956 4608059 4029038..4029346 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RS05100 of Ralstonia solanacearum of 50% (trembl|Q8Y1D7(SRS)). Has PF05957, Bacterial protein of unknown function (DUF883);IPR010279; This family consists of several hypothetical bacterial proteins of unknown function. no signal peptide. 1 TMH; Conserved hypothetical protein; hypothetical protein 4029346 4608059 azo3667 Azoarcus sp. BH72 hypothetical protein YP_935169.1 4029038 D 62928 CDS YP_935170.1 119899957 4608060 4029352..4029735 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rs05101 R. solanacearum of 36% (trembl|Q8Y1D8(SRS)). No domains predicted. No signal peptide. 2 TMHs; Conserved hypothetical protein; hypothetical protein 4029735 4608060 azo3668 Azoarcus sp. BH72 hypothetical protein YP_935170.1 4029352 D 62928 CDS YP_935171.1 119899958 4608061 4029732..4030103 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD74 of Azoarcus sp. EbN1 of 36% (gnl|keqq|eba:ebD74(KEGG)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 4030103 4608061 azo3669 Azoarcus sp. BH72 hypothetical protein YP_935171.1 4029732 D 62928 CDS YP_935172.1 119899959 4608062 4030246..4030803 1 NC_008702.1 Catalyzes the salvage synthesis of inosine-5'-monophosphate (IMP) and guanosine-5'-monophosphate (GMP) from the purine bases hypoxanthine and guanine, respectively; hypoxanthine-guanine phosphoribosyltransferase 4030803 hpt 4608062 hpt Azoarcus sp. BH72 hypoxanthine-guanine phosphoribosyltransferase YP_935172.1 4030246 D 62928 CDS YP_935173.1 119899960 4609027 4030814..4031149 1 NC_008702.1 Conserved hypothetical protein. Homology to BPP4351 of B.parapertussis of 38% (trembl:Q7W2Q3). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4031149 4609027 azo3671 Azoarcus sp. BH72 hypothetical protein YP_935173.1 4030814 D 62928 CDS YP_935174.1 119899961 4608063 4031270..4031950 1 NC_008702.1 DNA-binding response regulator,; High confidence in function and specificity; DNA-binding response regulator 4031950 qseB2 4608063 qseB2 Azoarcus sp. BH72 DNA-binding response regulator YP_935174.1 4031270 D 62928 CDS YP_935175.1 119899962 4609687 4032106..4033452 1 NC_008702.1 Two-component system histidine kinase,; High confidence in function and specificity; two-component system histidine kinase 4033452 qseC2 4609687 qseC2 Azoarcus sp. BH72 two-component system histidine kinase YP_935175.1 4032106 D 62928 CDS YP_935176.1 119899963 4609688 4033530..4034963 1 NC_008702.1 Probable serine protease MucD. Homology to mucD of P. aeruginosa of 51% (trembl|Q57155) Pfam: Trypsin; PDZ domain (Aslo konwn as DHR or GLGF) no TMH signal peptide; High confidence in function and specificity; serine protease MucD 4034963 mucD4 4609688 mucD4 Azoarcus sp. BH72 serine protease MucD YP_935176.1 4033530 D 62928 CDS YP_935177.1 119899964 4609286 4035014..4035511 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. Homology to bpp3105 of B. parapertussis of 20% (trembl|Q7W622). no domains predicted. no signal peptide. no TMHs; hypothetical protein 4035511 4609286 azo3675 Azoarcus sp. BH72 hypothetical protein YP_935177.1 4035014 D 62928 CDS YP_935178.1 119899965 4608064 complement(4035537..4036184) 1 NC_008702.1 Probable maleylpyruvate isomerase. Homology to nagL of Ralstonia spU of 50% (trembl|O86043) NagL is a reduced glutathione-dependent maleylpyruvate isomerase catalyzing the isomerization of maleypyruvate to fumarylpyruvate. InterPro: Glutathione S-transferase N terminus (IPR004045), Glutathione S-transferase C terminus (IPR004046) Pfam: Glutathione S-transferase, N-terminal domaine no signal peptide no TMHs; High confidence in function and specificity; maleylpyruvate isomerase 4036184 nagL2 4608064 nagL2 Azoarcus sp. BH72 maleylpyruvate isomerase YP_935178.1 4035537 R 62928 CDS YP_935179.1 119899966 4609314 complement(4036298..4037326) 1 NC_008702.1 TRAP-dicarboxylate transporter. Binds c4-dicarboxylates; part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. 23% TRAP_transptDctP. Pfam:PF03480; SBP_bac_7; 1. TIGRFAMs:TIGR00787; dctP; 1. Signal peptide: present.; Specificity unclear; putative c4-dicarboxylate-binding periplasmic protein 4037326 dctP6 4609314 dctP6 Azoarcus sp. BH72 putative c4-dicarboxylate-binding periplasmic protein YP_935179.1 4036298 R 62928 CDS YP_935180.1 119899967 4608607 complement(4037367..4038380) 1 NC_008702.1 This family consists of fumarylacetoacetate (FAA) hydrolase, or fumarylacetoacetate hydrolase (FAH) and it also includes HHDD isomerase/OPET decarboxylase from E. coli strain W. Similar to TREMBL:Q9KSB3 (67% identity); TREMBL:Q92LT4 (67% identity); TREMBL:Q8EGD4 (61% identity). Pfam (PF01557): Fumarylacetoacetate (FAA) hydrolase family.; Family membership; fumarylacetoacetate hydrolase family protein 4038380 4608607 azo3678 Azoarcus sp. BH72 fumarylacetoacetate hydrolase family protein YP_935180.1 4037367 R 62928 CDS YP_935181.1 119899968 4608065 complement(4038439..4038972) 1 NC_008702.1 Glyoxalase I (lactoylglutathione lyase) catalyzes the first step of the glyoxal pathway. Similar to trembl|Q89SS0 (63%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily; Family membership; glyoxalase-family protein 4038972 4608065 azo3679 Azoarcus sp. BH72 glyoxalase-family protein YP_935181.1 4038439 R 62928 CDS YP_935182.1 119899969 4608066 complement(4038995..4039219) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS01748 of R.solanacearum of 59% (trembl:Q8XRY0). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4039219 4608066 azo3680 Azoarcus sp. BH72 hypothetical protein YP_935182.1 4038995 R 62928 CDS YP_935183.1 119899970 4608067 complement(4039232..4040899) 1 NC_008702.1 Putative oxygenase. 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), InterPro: Aromatic-ring hydroxylase (flavoprotein monooxygenase); Specificity unclear; FAD-dependent oxidoreductase 4040899 mhpA 4608067 mhpA Azoarcus sp. BH72 FAD-dependent oxidoreductase YP_935183.1 4039232 R 62928 CDS YP_935184.1 119899971 4609217 complement(4040974..4041939) 1 NC_008702.1 Beta-lactamase II precursor (EC 3.5.2.6) (Penicillinase) (Cephalosporinase). Can hydrolyze carbapenem compounds. InterPro:IPR001279-Metallo-beta-lactamase superfamily TREMBL:Q8XRX8-72% identity TREMBL:Q7VUG6- 62% Pfam:PF00753-LactamaseB, Ribosomal proteinS6e,ThiC family TIGRFAM:TIGR00010- TatD/hisF family TMHMM predicted transmembrane helix. mobB: molybdopterin-guanine dinucleotide; High confidence in function and specificity; putative cyclase 4041939 4609217 azo3682 Azoarcus sp. BH72 putative cyclase YP_935184.1 4040974 R 62928 CDS YP_935185.1 119899972 4608068 4042117..4042941 1 NC_008702.1 Putative transcriptional regulator iclR-family,; Family membership; IclR family transcriptional regulator 4042941 gylR 4608068 gylR Azoarcus sp. BH72 IclR family transcriptional regulator YP_935185.1 4042117 D 62928 CDS YP_935186.1 119899973 4608974 4043053..4043889 1 NC_008702.1 Putative periplasmic binding protein-related protein.This family includes several of which are involved in iron transport. 24%; Function unclear; putative periplasmic binding protein 4043889 4608974 azo3684 Azoarcus sp. BH72 putative periplasmic binding protein YP_935186.1 4043053 D 62928 CDS YP_935187.1 119899974 4608069 4043889..4047515 1 NC_008702.1 Putative hybrid sensor and regulator protein, MmoS) InterPro: IPR003594; ATPbind_ATPase. IPR005467; His_kinase. IPR003661; His_kinA_N. IPR008207; Hpt. IPR001610; PAC. IPR000700; PAS-assoc_C. IPR000014; PAS_domain. IPR001789; Response_reg. Pfam: PF02518; HATPase_c. PF00512; HisKA. PF00785; PAC. PF00989; PAS. PF00072; Response_reg. SMART: SM00387; HATPase_c. SM00388; HisKA. SM00073; HPT. SM00086; PAC. SM00091; PAS. SM00448; REC. TIGRFAM: TIGR00229; sensory_box. TMHMM reporting 2 transmembrane helices.; Family membership; putative hybrid sensor and regulator protein 4047515 4608069 azo3685 Azoarcus sp. BH72 putative hybrid sensor and regulator protein YP_935187.1 4043889 D 62928 CDS YP_935188.1 119899975 4608070 4047603..4048715 1 NC_008702.1 Probable response regulator, HD. IPR003607; Met_phsphohydro. IPR001789; Response_reg. IPR008328; Res_reg_HDGYP. Pfam: PF01966; HD. PF00072; Response_reg. SMART: SM00471; HDc. SM00448; REC.; Specificity unclear; response regulator 4048715 rpfG 4608070 rpfG Azoarcus sp. BH72 response regulator YP_935188.1 4047603 D 62928 CDS YP_935189.1 119899976 4609759 complement(4048737..4049372) 1 NC_008702.1 Putative cytochrome c4. Homology to cc4 of A. vinelandii of 29% (sprot|CYC4_AZOVI). Diheme, high potential cytochrome c believed to be an intermediate electron donor to terminal oxidation systems. Pfam: cytochrome C signal peptide no TMHs; putative cytochrome c4 4049372 cc43 4609759 cc43 Azoarcus sp. BH72 putative cytochrome c4 YP_935189.1 4048737 R 62928 CDS YP_935190.1 119899977 4608440 complement(4049428..4049787) 1 NC_008702.1 Conserved hypothetical protein. Homology to pspto0091 of P. syringae of 35% (trembl|Q88BC6). Pfam: DUF861. This family consists of several proteins which seem to be specific to plants and bacteria. The function of this family is unknown. no signal peptide. no TMHs; hypothetical protein 4049787 4608440 azo3688 Azoarcus sp. BH72 hypothetical protein YP_935190.1 4049428 R 62928 CDS YP_935191.1 119899978 4608071 complement(4049799..4050842) 1 NC_008702.1 catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis; UDP-N-acetylenolpyruvoylglucosamine reductase 4050842 murB 4608071 murB Azoarcus sp. BH72 UDP-N-acetylenolpyruvoylglucosamine reductase YP_935191.1 4049799 R 62928 CDS YP_935192.1 119899979 4609288 complement(4050942..4051349) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to atu1643 of A. tymefaciens of 39% (trembl|Q8UEW1(SRS)). Pfam: Bacterial transmembrane pair family. This domain represents a conserved pair of transmembrane helices. It appears to be found as two tandem repeats in a family of hypothetical proteins. signal peptide. 3 TMHs; Conserved hypothetical protein; hypothetical protein 4051349 4609288 azo3690 Azoarcus sp. BH72 hypothetical protein YP_935192.1 4050942 R 62928 CDS YP_935193.1 119899980 4608072 complement(4051624..4053654) 1 NC_008702.1 Putative methyl-accepting chemotaxis protein,Chmtaxis_transd. IPR003660; HAMP. Pfam: PF00672; HAMP. PF00015; MCPsignal. SMART: SM00304; HAMP. SM00283; MA. Signal P reporting signal peptide. TMHMM reporting 2 transmembrane helices.; Family membership; putative methyl-accepting chemotaxis transducer 4053654 4608072 azo3691 Azoarcus sp. BH72 putative methyl-accepting chemotaxis transducer YP_935193.1 4051624 R 62928 CDS YP_935194.1 119899981 4608073 4053784..4054350 1 NC_008702.1 Putative N-acetyltransferase,51% Identity to TrEMBl;Q7WQB1,54% Identity to TrEMBL;Q63LT2,Q7WCA7. Has PF00583, Acetyltransferase (GNAT) family;IPR000182,GCN5acetyl_trans; This family contains proteins with N-acetyltransferase functions. Histone acetylation is carried out by a class of enzymes known as histone acetyltransferases (HATs), which catalyze the transfer of an acetyl group from acetyl-CoA to the lysine E -amino groups on the N-terminal tails of histones. Early indication that HATs were involved in transcription came from the observation that in actively transcribed regions of chromatin, histones tend to be hyperacetylated, whereas in transcriptionally silent regions histones are hypoacetylated. The histone acetyltransferases are divided into five families. These include the Gcn5-related acetyltransferases (GNATs); the MYST (for `MOZ, Ybf2/Sas3,Sas2 and Tip60)-related HATs; p300/CBP HATs; the general transcription factor HATs, which include the TFIID subunit TAF250; and the nuclear hormone-related HATs SRC1 and ACTR (SRC3). The GCN5-related N-acetyltransferase superfamily includes such enzymes as the histone acetyltransferases GCN5 and Hat1, the elongator complex subunit Elp3, the mediator-complex subunit Nut1, and Hpa2; putative N-acetyltransferase 4054350 4608073 azo3692 Azoarcus sp. BH72 putative N-acetyltransferase YP_935194.1 4053784 D 62928 CDS YP_935195.1 119899982 4608074 4054347..4055573 1 NC_008702.1 Similar to hypothetical integral membrane transport protein yidY (MFS family). Signal peptide containing non secretory protein. With 12 putative transmembrane helices. General transport of carbohydrates , amino acids, and other small molecules.; Conserved hypothetical protein; hypothetical protein 4055573 4608074 azo3693 Azoarcus sp. BH72 hypothetical protein YP_935195.1 4054347 D 62928 CDS YP_935196.1 119899983 4608075 4055667..4055852 1 NC_008702.1 Hypothetical secreted protein. No good homology to the data bank. Has Signal Peptide.; hypothetical protein 4055852 4608075 azo3694 Azoarcus sp. BH72 hypothetical protein YP_935196.1 4055667 D 62928 CDS YP_935197.1 119899984 4608076 complement(4056007..4057344) 1 NC_008702.1 catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; tryptophanyl-tRNA synthetase 4057344 trpS 4608076 trpS Azoarcus sp. BH72 tryptophanyl-tRNA synthetase YP_935197.1 4056007 R 62928 CDS YP_935198.1 119899985 4610037 complement(4057400..4057786) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to bb3068 of B. bronchiseptica of 31% (trembl|Q7WHY8(SRS)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 4057786 4610037 azo3696 Azoarcus sp. BH72 hypothetical protein YP_935198.1 4057400 R 62928 CDS YP_935199.1 119899986 4608077 complement(4057811..4058158) 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. No TMHs. Has signal peptide.; hypothetical protein 4058158 4608077 azo3697 Azoarcus sp. BH72 hypothetical protein YP_935199.1 4057811 R 62928 CDS YP_935200.1 119899987 4608078 4058405..4058953 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03001371 of Dechloromonas aromatica of 43% (gi|53730748|ref|ZP_00348943.1|(NBCI ENTREZ)). No doamins predicted. No signal peptide. No TMHs.; hypothetical protein 4058953 4608078 azo3698 Azoarcus sp. BH72 hypothetical protein YP_935200.1 4058405 D 62928 CDS YP_935201.1 119899988 4608079 4058961..4060172 1 NC_008702.1 Conserved hypothetical protein, 38% identity(51% similarity) to TrEMBL;Q8KAU5. TrEMBL; Q6MRD6(59% identity). Has PF07075, Protein of unknown function (DUF1343);IPR008302, UCP016719; This family consists of several hypothetical bacterial proteins of around 400 residues in length. The function of this family is unknown.There are currently no experimental data for members of this group or their homologues, nor do they exhibit features indicative of any function.; Function unclear; hypothetical protein 4060172 4608079 azo3699 Azoarcus sp. BH72 hypothetical protein YP_935201.1 4058961 D 62928 CDS YP_935202.1 119899989 4608080 complement(4060198..4060578) 1 NC_008702.1 Probable thioredoxin-disulfide reductase. Homology to trxC3 (THI3_CORNE) of C. nephridii of 43%. Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Pfam: Thioredoxin no signal peptide no TMHs; Family membership; thioredoxin-disulfide reductase 4060578 trxC2 4608080 trxC2 Azoarcus sp. BH72 thioredoxin-disulfide reductase YP_935202.1 4060198 R 62928 CDS YP_935203.1 119899990 4610044 4060726..4061031 1 NC_008702.1 Conserved hypothetical protein. Homology to bll7423 of B. japonicum of 53% (trembl|Q89DL6). Pfam: Antibiotic biosynthesis monooxygenase. no TMHs. No signal peptide.; hypothetical protein 4061031 4610044 azo3701 Azoarcus sp. BH72 hypothetical protein YP_935203.1 4060726 D 62928 CDS YP_935204.1 119899991 4608081 4061111..4061821 1 NC_008702.1 conserved hypothetical protein. Homology to Avin02001428 of Azotobacter vinelandii of 54% (gi|53612536|ref|ZP_00091632.2|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 4061821 4608081 azo3702 Azoarcus sp. BH72 hypothetical protein YP_935204.1 4061111 D 62928 CDS YP_935205.1 119899992 4608082 complement(4061834..4062295) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to PM2013 of P. multocida of 44% (tremble:Q9CJJ4). probable signal peptide. probable 2 TMHS; Conserved hypothetical protein; hypothetical protein 4062295 4608082 azo3703 Azoarcus sp. BH72 hypothetical protein YP_935205.1 4061834 R 62928 CDS YP_935206.1 119899993 4608083 4062391..4063305 1 NC_008702.1 Hypothetical membrane protein. No homology of the entire protein to the data bank. Has PF05875;Alkaline phytoceramidase (aPHC):This family consists of several eukaryotic Alkaline phytoceramidase (aPHC) sequences. Ceramidases are enzymes involved in regulating cellular levels of ceramides, sphingoid bases, and their phosphates. Alkaline phytoceramidase (aPHC) is responsible for the hydrolysis of phytoceramide. No signal peptide. 6 TMHs.; hypothetical protein 4063305 4608083 azo3704 Azoarcus sp. BH72 hypothetical protein YP_935206.1 4062391 D 62928 CDS YP_935207.1 119899994 4608084 complement(4063257..4064321) 1 NC_008702.1 Putative AraC family transcriptional regulator,; High confidence in function and specificity; AraC family transcriptional regulator 4064321 gapR 4608084 gapR Azoarcus sp. BH72 AraC family transcriptional regulator YP_935207.1 4063257 R 62928 CDS YP_935208.1 119899995 4608860 4064470..4065540 1 NC_008702.1 Similar to TREMBL:Q89RW7 (50% identity,64% similarity); TREMBL:Q88IS2 (49% identity). InterPro (IPR000051); SAM (and some other nucleotide) binding motif.; hypothetical protein 4065540 4608860 azo3706 Azoarcus sp. BH72 hypothetical protein YP_935208.1 4064470 D 62928 CDS YP_935209.1 119899996 4608085 complement(4065556..4065882) 1 NC_008702.1 Conserved hypothetical protein. Homology to sty1393 of S. typhi of 34% (trembl|Q8Z792). Pfam: Antibiotic biosynthesis monooxygenase. no signal peptide. no TMHs; hypothetical protein 4065882 4608085 azo3707 Azoarcus sp. BH72 hypothetical protein YP_935209.1 4065556 R 62928 CDS YP_935210.1 119899997 4608086 complement(4065983..4066393) 1 NC_008702.1 Dioxygenases catalyze the incorporation of both atoms of molecular oxygen into substrates. Cleavage of aromatic rings is one of the most important function of dioxygenases. Similar to trembl|Q7WJ14 (50%). Pfam (PF00903): Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily; Family membership; dioxygenase-family protein 4066393 4608086 azo3708 Azoarcus sp. BH72 dioxygenase-family protein YP_935210.1 4065983 R 62928 CDS YP_935211.1 119899998 4608087 complement(4066490..4066942) 1 NC_008702.1 Family membership; ORF8; hypothetical protein 4066942 4608087 azo3709 Azoarcus sp. BH72 hypothetical protein YP_935211.1 4066490 R 62928 CDS YP_935212.1 119899999 4608088 complement(4067068..4067532) 1 NC_008702.1 Conserved hypothetical transferase. Homology to the IAA acetyltransferase of A. brasilense of 37% (sprot|IAAT_AZOBR). The IAA acetyltransferase participates in the tryptophan-dependent indole-3-acetic acid production, which is a phytohormone released by A.brasilense. Interpro: GCN5-related N-acetyltransferase (IPR000182). Pfam: Acetyltransferase (GNAT) family. no signal peptide. no TMHs; Conserved hypothetical protein; acetyltransferase 4067532 4608088 azo3710 Azoarcus sp. BH72 acetyltransferase YP_935212.1 4067068 R 62928 CDS YP_935213.1 119900000 4608089 complement(4067656..4068402) 1 NC_008702.1 Similar to SWISSPROT:P39367 (68% identity),TREMBL:Q8Z1C8 (66% identity).; hypothetical protein 4068402 4608089 azo3711 Azoarcus sp. BH72 hypothetical protein YP_935213.1 4067656 R 62928 CDS YP_935214.1 119900001 4608090 complement(4068452..4068979) 1 NC_008702.1 conserved hypothetical protein. Homology to Raeut03002407 of Ralstonia eutropha of 48% (gi|46132577|ref|ZP_00171131.2|(NBCI ENTREZ)). Pfam: NUDIX domain. No signal peptide. No TMHs.; hypothetical protein 4068979 4608090 azo3712 Azoarcus sp. BH72 hypothetical protein YP_935214.1 4068452 R 62928 CDS YP_935215.1 119900002 4608091 complement(4069022..4069390) 1 NC_008702.1 Bleomycin (Blm) is a glycopeptide antibiotic produced naturally by actinomycetes. It is a strong DNA-cutting agent and thus finds use as a potent anti-cancer drug. Actinomycetes have developed a defence mechanism against this lethal compound, producing a protein that confers resistance to Blm through drug sequestering.Putative Bleomycin resistance protein,; Family membership; putative bleomycin resistance protein 4069390 bleO 4608091 bleO Azoarcus sp. BH72 putative bleomycin resistance protein YP_935215.1 4069022 R 62928 CDS YP_935216.1 119900003 4608399 complement(4069873..4070424) 1 NC_008702.1 Probable Acetyltransferase. Has weak homology with hits in the database, TrEMBL;Q7N1H0(56% Identity). Has PF00583, Acetyltransferase (GNAT) family; IPR000182, GCN5acetyl_trans; This family contains proteins with N-acetyltransferase functions.;Histone acetylation is carried out by a class of enzymes known as histone acetyltransferases (HATs), which catalyze the transfer of an acetyl group from acetyl-CoA to the lysine E -amino groups on the N-terminal tails of histones. Early indication that HATs were involved in transcription came from the observation that in actively transcribed regions of chromatin, histones tend to be hyperacetylated, whereas in transcriptionally silent regions histones are hypoacetylated. The histone acetyltransferases are divided into five families. These include the Gcn5-related acetyltransferases (GNATs); the MYST (for `MOZ, Ybf2/Sas3,Sas2 and Tip60)-related HATs; p300/CBP HATs; the general transcription factor HATs, which include the TFIID subunit TAF250; and the nuclear hormone-related HATs SRC1 and ACTR (SRC3). The GCN5-related N-acetyltransferase superfamily includes such enzymes as the histone acetyltransferases GCN5 and Hat1, the elongator complex subunit Elp3, the mediator-complex subunit Nut1, and Hpa2; Family membership; acetyltransferase 4070424 4608399 azo3714 Azoarcus sp. BH72 acetyltransferase YP_935216.1 4069873 R 62928 CDS YP_935217.1 119900004 4608092 complement(4070525..4070932) 1 NC_008702.1 Hypothetical membrane protein. Homology to an orf of E. coli of 29% (trembl|Q8VNQ4). no domains predricted. no signal peptide. 3 TMHs; hypothetical protein 4070932 4608092 azo3715 Azoarcus sp. BH72 hypothetical protein YP_935217.1 4070525 R 62928 CDS YP_935218.1 119900005 4608093 complement(4071043..4071537) 1 NC_008702.1 hypothetical protein 4071537 4608093 azo3716 Azoarcus sp. BH72 hypothetical protein YP_935218.1 4071043 R 62928 CDS YP_935219.1 119900006 4608094 complement(4071620..4071769) 1 NC_008702.1 Region start changed from 4071796 to 4071769 (-27 bases), , Changed start from att to next atg; hypothetical protein 4071769 4608094 azo3717 Azoarcus sp. BH72 hypothetical protein YP_935219.1 4071620 R 62928 CDS YP_935220.1 119900007 4608095 complement(4071881..4072303) 1 NC_008702.1 hypothetical protein 4072303 4608095 azo3718 Azoarcus sp. BH72 hypothetical protein YP_935220.1 4071881 R 62928 CDS YP_935221.1 119900008 4608096 complement(4072411..4072626) 1 NC_008702.1 hypothetical protein 4072626 4608096 azo3719 Azoarcus sp. BH72 hypothetical protein YP_935221.1 4072411 R 62928 CDS YP_935222.1 119900009 4608097 4072842..4073585 1 NC_008702.1 Putative truncated transposase for insertion sequence element IS1328. Required for the transposition of the insertion element (Potential). Homology only to the N-terminus.; Family membership; transposase 4073585 4608097 azo3720 Azoarcus sp. BH72 transposase YP_935222.1 4072842 D 62928 CDS YP_935223.1 119900010 4608098 4073607..4073966 1 NC_008702.1 Putative truncated transposase for insertion sequence element IS1328. Required for the transposition of the insertion element (Potential). InterPro: Transposase IS116/IS110/IS902 family. Homoloy only to the C-terminus.; Specificity unclear; transposase 4073966 4608098 azo3721 Azoarcus sp. BH72 transposase YP_935223.1 4073607 D 62928 CDS YP_935224.1 119900011 4608099 complement(4076853..4077803) 1 NC_008702.1 Hypothetical protein, Has very weak or no homologs in the Database. Has No domains, repeats, motifs or features predicted above threshold score.; hypothetical protein 4077803 4608099 azo3722 Azoarcus sp. BH72 hypothetical protein YP_935224.1 4076853 R 62928 CDS YP_935225.1 119900012 4608100 complement(4077851..4078369) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 4078369 4608100 azo3723 Azoarcus sp. BH72 hypothetical protein YP_935225.1 4077851 R 62928 CDS YP_935226.1 119900013 4608101 4078566..4079330 1 NC_008702.1 Truncated integrase/recombinase, probably Integrase/recombinase. 58% Identity to SProt;P62591,P62592. Has PF00589, Phage integrase family; IPR002104, Phage_integrase; Members of this family cleave DNA substrates by a series of staggered cuts, during which the protein becomes covalently linked to the DNA through a catalytic tyrosine residue at the carboxy end of the alignment. The catalytic site residues in CRE recombinase (P06956) are Arg-173, His-289, Arg-292 and Tyr-324. Homology only to the N-terminus; truncated integrase/recombinase 4079330 4608101 azo3724 Azoarcus sp. BH72 truncated integrase/recombinase YP_935226.1 4078566 D 62928 CDS YP_935227.1 119900014 4608102 4079327..4079527 1 NC_008702.1 Putative truncated integrase/recombinase (E2 protein). PUTATIVE INTEGRASE BELIEVED TO BE INVOLVED IN INSERTIONS OF ANTIBIOTIC RESISTANCE GENES INTO PLASMIDS AND TRANSPOSONS. Homology only to the C-terminus.; Family membership; integrase 4079527 4608102 azo3725 Azoarcus sp. BH72 integrase YP_935227.1 4079327 D 62928 CDS YP_935228.1 119900015 4608103 complement(4079534..4083235) 1 NC_008702.1 one of two methionine synthases in Escherichia coli; MetH catalyzes a methyl transfer reaction from methyltetrahydrofolate to homocysteine to create methionine; requires zinc for activity; B12-dependent methionine synthase 4083235 metH 4608103 metH Azoarcus sp. BH72 B12-dependent methionine synthase YP_935228.1 4079534 R 62928 CDS YP_935229.1 119900016 4609209 complement(4083455..4084066) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to ebA3180 of Azoarcus sp. EbN1 of 57% (gi|56477224|ref|YP_158813.1|(NBCI ENTREZ)). No domains predicted Signal Peptide present. No TMH reported present.; Conserved hypothetical protein; hypothetical protein 4084066 4609209 azo3727 Azoarcus sp. BH72 hypothetical protein YP_935229.1 4083455 R 62928 CDS YP_935230.1 119900017 4608104 complement(4084068..4084556) 1 NC_008702.1 Probable lipoprotein, 58% similarity to TrEMBL:Q8XWT8 PROBABLE LIPOPROTEIN [RS01163] [Ralstonia solanacearum (Pseudomonas solanacearum)]. Has PF04355:IPR007450:SmpA / OmlA family;Lipoprotein Bacterial outer membrane lipoprotein, possibly involved in in maintaining the structural integrity of the cell envelope [1]. Lipid attachment site is a conserved N terminal cysteine residue. Sometimes found adjacent to the OmpA domain (OmpA). Signal peptide present. NO TMH reported present.; Family membership; lipoprotein 4084556 4608104 azo3728 Azoarcus sp. BH72 lipoprotein YP_935230.1 4084068 R 62928 CDS YP_935231.1 119900018 4608105 4084668..4085792 1 NC_008702.1 In enteric bacteria such as E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar to sprot|LIVK_ECOLI (31%) and to trembl|Q7NV64 (54%). Pfam (PF01094): Receptor family ligand binding region Pfam (PF00861): Ribosomal L18p/L5e family SignalP reporting Signal peptide.; Specificity unclear; leucine-specific binding protein 4085792 livK3 4608105 livK3 Azoarcus sp. BH72 leucine-specific binding protein YP_935231.1 4084668 D 62928 CDS YP_935232.1 119900019 4609155 4086174..4087289 1 NC_008702.1 In enteric bacteria such as E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar to trembl|Q7NV64 (58%) and to sprot|LIVK_ECOLI (33%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; leucine-specific binding protein 4087289 livK4 4609155 livK4 Azoarcus sp. BH72 leucine-specific binding protein YP_935232.1 4086174 D 62928 CDS YP_935233.1 119900020 4609156 4087402..4088334 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar trembl|Q7NXX3 (60%), to sprot|BRAD_PSEAE (47%) and to sprot|LIVH_ECOLI (44%). Pfam (PF02653): Branched-chain amino acid transport system / permease component TMHMM reporting seven Tmhelix. gntP: gluconate transporter; Specificity unclear; ABC transporter permease 4088334 livH 4609156 livH Azoarcus sp. BH72 ABC transporter permease YP_935233.1 4087402 D 62928 CDS YP_935234.1 119900021 4609151 4088347..4089444 1 NC_008702.1 Putative branched-chain amino acid transport permease. Homology to livM of S. typhimurium of 44%. Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. no signal peptide probable 8 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 4089444 livM5 4609151 livM5 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_935234.1 4088347 D 62928 CDS YP_935235.1 119900022 4609162 4089441..4090214 1 NC_008702.1 Probable high-affinity branched-chain amino acid transport ATP-binding protein BraF. ATP-binding cassette (ABC) transporters are multidomain membrane proteins,responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. BraF is a COMPONENT OF THE HIGH AFFINITY LEUCINE ISOLEUCINE VALINE TRANSPORT SYSTEM (LIV-I) WHICH IS OPERATIVE WITHOUT NA(+) AND IS SPECIFIC FOR ALANINE AND THREONINE IN ADDITION TO BRANCHED-CHAIN AMINO ACIDS. Similar to trembl|Q7NXX1 (67%), to sprot|BRAF_PSEAE (45%) and to sprot|LIVG_ECOLI (44%). Pfam (PF00005): ABC transporter Smart (SM00382): AAA ATPase superfamily ProSite (PS50101): ATP/GTP-binding site motif A (P-loop); Specificity unclear; ABC transporter ATP-binding protein 4090214 livG2 4609162 livG2 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_935235.1 4089441 D 62928 CDS YP_935236.1 119900023 4609150 4090204..4090938 1 NC_008702.1 ATP-binding cassette (ABC) transporters are multidomain membrane proteins, responsible for the controlled efflux and influx of substances (allocrites) across cellular membranes. ATP-binding protein is for coupling the energy of ATP hydrolysis to conformational changes in the transmembrane domains. Similar to trembl|Q7NXX0 (58%) and to trembl|Q7VVD3 (59%). Smart: AAA ATPases; Specificity unclear; ABC transporter ATP-binding protein 4090938 4609150 azo3734 Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_935236.1 4090204 D 62928 CDS YP_935237.1 119900024 4608106 complement(4090917..4092113) 1 NC_008702.1 Bicyclomycin resistance protein homolog. Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase (By similarity). InterPro: General carbohydrate, amino acid , and divalent cation transport. Occurence of signal peptide and 12 transmembrane helices. Presence of DUF domains and Cation transport ATPase C-terminus domains. 35% identity and 47% similarity to bcr from X.axonopodis. Probably it is a copy of bcr1 in Azoarcus BH72 genome.; Conserved hypothetical protein; putative bicyclomycin resistance protein 4092113 bcr2 4608106 bcr2 Azoarcus sp. BH72 putative bicyclomycin resistance protein YP_935237.1 4090917 R 62928 CDS YP_935238.1 119900025 4608383 complement(4092166..4093665) 1 NC_008702.1 Conserved hypothetical protein. Homology to rsc0338 of R. solanacearum of 64% (trembl|Q8Y2J7). Probable Mg(2+) chelatase family protein. InterPro: Mg chelatase-related protein (IPR004482); MCM family (IPR001208); AAA ATPase superfamily (IPR003593); Magnesium chalatase, chlI subunit (IPR000523); TypeI antifreeze protein (IPR000104). no signal peptide. no TMHs. TIGR00368: Mg chelatase-related protein; Family membership; hypothetical protein 4093665 4608383 azo3736 Azoarcus sp. BH72 hypothetical protein YP_935238.1 4092166 R 62928 CDS YP_935239.1 119900026 4608107 complement(4093748..4093999) 1 NC_008702.1 Conserved hypothetical protein. Homology to NE0192 of N.europaea of 52% (trembl:Q82XR2). Has PF04380, Protein of unknown function (DUF526);IPR007475 ;This is a family of uncharacterised proteins. No signal peptide. No TMHs.; putative secreted protein 4093999 4608107 azo3737 Azoarcus sp. BH72 putative secreted protein YP_935239.1 4093748 R 62928 CDS YP_935240.1 119900027 4608108 4094284..4095024 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rsc0341 of R. solanacearum of 40% (trembl|Q8Y2J4(SRS)). no domains predicted. signal peptide. no TMHs; hypothetical protein 4095024 4608108 azo3738 Azoarcus sp. BH72 hypothetical protein YP_935240.1 4094284 D 62928 CDS YP_935241.1 119900028 4608109 4095083..4095421 1 NC_008702.1 PII-like signal transmitter proteins are involved in the regulation of ammonium assimilation and nitrogen fixation. The PII-like proteins differed from each other in details of N-sensing. They were covalently modified by uridylylation upon nitrogen limitation. Similar to trembl|Q9EZQ3 (100%) and to trembl|Q7NQY3 (87%). Pfam: Nitrogen regulatory protein P-II; High confidence in function and specificity; PII-like signal transmitter protein GlnK 4095421 glnK 4608109 glnK Azoarcus sp. BH72 PII-like signal transmitter protein GlnK YP_935241.1 4095083 D 62928 CDS YP_935242.1 119900029 4608899 4095436..4096842 1 NC_008702.1 Ammonium transporter 1 member 1 (AtAMT1;1). Involved in high affinity ammonium uptake. TREMBL:Q8RP89: 100% identity (published) InterPro:IPR001905; Ammonium_transpt. IPR002229; RhesusRHD. IPR010256; RH_like_transpt. Pfam: PF00909; Ammonium_transp PRINTS PR00342; RHESUSRHD. TIGRFAMs TIGR00836; amt; PROSITE: PS01219; AMMONIUM_TRANSP SignalP predicted signal peptide present (0.997 probability). TMHMM predicted 12 transmembrane helices; High confidence in function and specificity; ammonium transporter 4096842 amtB 4608899 amtB Azoarcus sp. BH72 ammonium transporter YP_935242.1 4095436 D 62928 CDS YP_935243.1 119900030 4608316 4097017..4097757 1 NC_008702.1 Outer membrane protein W precursor. Acts as a receptor for colicin S4.; High confidence in function and specificity; outer membrane protein 4097757 ompW 4608316 ompW Azoarcus sp. BH72 outer membrane protein YP_935243.1 4097017 D 62928 CDS YP_935244.1 119900031 4609449 complement(4097840..4098715) 1 NC_008702.1 produces formate from formyl-tetrahydrofolate which is the major source of formate for PurT in de novo purine nucleotide biosynthesis; has a role in one-carbon metabolism; forms a homohexamer; activated by methionine and inhibited by glycine; formyltetrahydrofolate deformylase 4098715 purU 4609449 purU Azoarcus sp. BH72 formyltetrahydrofolate deformylase YP_935244.1 4097840 R 62928 CDS YP_935245.1 119900032 4609665 complement(4098817..4099428) 1 NC_008702.1 catalyzes the formation of serine from phosphoserine; also has phosphoserine:homoserine phosphotransferase activity; phosphoserine phosphatase 4099428 thrH 4609665 thrH Azoarcus sp. BH72 phosphoserine phosphatase YP_935245.1 4098817 R 62928 CDS YP_935246.1 119900033 4609945 4099596..4100801 1 NC_008702.1 HD-domain containing protein,; Conserved hypothetical protein; HD-domain-containing protein 4100801 4609945 azo3744 Azoarcus sp. BH72 HD-domain-containing protein YP_935246.1 4099596 D 62928 CDS YP_935247.1 119900034 4608110 complement(4100816..4102747) 1 NC_008702.1 ABC transporter ATP-binding protein yheS. Nonsecretory protein with Signal peptide. Presence of oxidoreductase Molybdopterin binding domain, M-protein repeats, RecF protein domains, Cu amine oxidase N3 domain,K+ channels and iron-sulphur binding potentials.57% identity and 68% similarity to Dechloromonas ATPase component of ABC transporter ureG: urease accessory protein UreG; Family membership; ABC transporter ATP-binding protein 4102747 yheS 4608110 yheS Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_935247.1 4100816 R 62928 CDS YP_935248.1 119900035 4610182 4102815..4103438 1 NC_008702.1 Hypothetical secreted protein. No homology to the data bank. No domains predicted. signal peptide no TMHs; hypothetical protein 4103438 4610182 azo3746 Azoarcus sp. BH72 hypothetical protein YP_935248.1 4102815 D 62928 CDS YP_935249.1 119900036 4608111 complement(4103463..4104068) 1 NC_008702.1 Catalyzes the isomerization of 2-hydroxychromene-2-carboxylate (hcca) to trans-o-hydroxybenzylidenepyruvate (thbpa). Upper napthalene catabolic pathway which involves conversion of napthalene to salicylate. Similar to TREMBL:Q7WEK4 (57% identity); TREMBL:Q9I714 (35% identity); SWISSPROT:Q51948 (29% identity). InterPro: 2-hydroxychromene-2-carboxylate isomerase Pfam (HCCA_isomerase): 2-hydroxychromene-2-carboxylate isomerase.; Specificity unclear; 2-hydroxychromene-2-carboxylate isomerase 4104068 4608111 azo3747 Azoarcus sp. BH72 2-hydroxychromene-2-carboxylate isomerase YP_935249.1 4103463 R 62928 CDS YP_935250.1 119900037 4608112 complement(4104098..4104472) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 4104472 4608112 azo3748 Azoarcus sp. BH72 hypothetical protein YP_935250.1 4104098 R 62928 CDS YP_935251.1 119900038 4608113 4104611..4105519 1 NC_008702.1 Probable HTH-type transcriptional activator ampR. Regulates the expression of the beta-lactamase gene. Represses cephalosporinase (ampC) in the presence of beta-lactams and induces it in the absence of them. Similar to SWISSPROT: sprot|AMPR_PSEAE (34% Pseudomonas aeruginosa,HTH-type transcriptional activator AmpR) Pfam: PF00126 Bacterial regulatory helix-turn-helix protein, lysR family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; LysR family transcriptional regulator 4105519 ampR 4608113 ampR Azoarcus sp. BH72 LysR family transcriptional regulator YP_935251.1 4104611 D 62928 CDS YP_935252.1 119900039 4608315 4105616..4106194 1 NC_008702.1 This is a family of hydrolase enzymes. Isochorismatase, also known as 2,3 dihydro-2,3 dihydroxybenzoatesynthase catalyses the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate. Similar to TREMBL:Q7NWS7 (61% identity); TREMBL:Q8UIM8 (57% identity); TREMBL:Q89UX3 (33% identity). InterPro (IPR000868): Isochorismatase hydrolase. Pfam (PF00857): Isochorismatase family.; Family membership; isochorismatase family protein 4106194 4608315 azo3750 Azoarcus sp. BH72 isochorismatase family protein YP_935252.1 4105616 D 62928 CDS YP_935253.1 119900040 4608114 4106205..4107167 1 NC_008702.1 Probable AraC family transcriptional regulator,; High confidence in function and specificity; AraC family transcriptional regulator 4107167 darR 4608114 darR Azoarcus sp. BH72 AraC family transcriptional regulator YP_935253.1 4106205 D 62928 CDS YP_935254.1 119900041 4608588 complement(4107175..4108071) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to RSC0519 of R. solanacearum of 51%. InterPro: Integral membrane protein DUF6. Tigrfam: 2A78: Carboxylate/Amino Acid/Amine Transporter. Pfam: Integral membrane protein DUF6. probable signal peptide. probable 9 TMHs; Conserved hypothetical protein; hypothetical protein 4108071 4608588 azo3752 Azoarcus sp. BH72 hypothetical protein YP_935254.1 4107175 R 62928 CDS YP_935255.1 119900042 4608115 complement(4108137..4111802) 1 NC_008702.1 proline utilization protein A; multifunctional protein that functions in proline catabolism in the first two enzymatic steps resulting in the conversion of proline to glutamate; in Escherichia coli this protein self regulates transcription via a DNA-binding domain at the N-terminus but the protein from Pseudomonas does not have this domain; bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase 4111802 putA 4608115 putA Azoarcus sp. BH72 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase YP_935255.1 4108137 R 62928 CDS YP_935256.1 119900043 4609666 complement(4111993..4113042) 1 NC_008702.1 Putative thiamine biosynthesis lipoprotein ApbE. Homology to apbE of Salmonella typhimurium of 27% (gi|5915687|sp|P41780|APBE_SALTY(NBCI ENTREZ)). Pfam: ApbE family. This prokaryotic family of lipoproteins are related to ApbE from Salmonella typhimurium. ApbE is involved in thiamine synthesis. More specifically is may be involved in the conversion of aminoimidazole ribotide (AIR) to 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP). No TMHs. Signal peptide present.; Family membership; putative thiamine biosynthesis lipoprotein ApbE 4113042 4609666 azo3754 Azoarcus sp. BH72 putative thiamine biosynthesis lipoprotein ApbE YP_935256.1 4111993 R 62928 CDS YP_935257.1 119900044 4608116 complement(4113039..4113995) 1 NC_008702.1 catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione; glutathione synthetase 4113995 gshB 4608116 gshB Azoarcus sp. BH72 glutathione synthetase YP_935257.1 4113039 R 62928 CDS YP_935258.1 119900045 4608945 complement(4114008..4115303) 1 NC_008702.1 glutamate--cysteine ligase. Glutathione metabolism.; High confidence in function and specificity; GshA protein 4115303 gshA 4608945 gshA Azoarcus sp. BH72 GshA protein YP_935258.1 4114008 R 62928 CDS YP_935259.1 119900046 4608944 complement(4115424..4116680) 1 NC_008702.1 Modification methylase TaqI (EC 2.1.1.72) (Adenine-specific methyltransferase TaqI) (M.TaqI). THIS METHYLASE RECOGNIZES THE DOUBLE-STRANDED SEQUENCE TCGA CAUSES SPECIFIC METHYLATION ON A-4 ON BOTH STRANDS AND PROTECTS THE DNA FROM CLEAVAGE BY THE TAQI ENDONUCLEASE. InterPro: N6 adenine-specific DNA methyltransferase N12 class; Family membership; modification methyltransferase 4116680 4608944 azo3757 Azoarcus sp. BH72 modification methyltransferase YP_935259.1 4115424 R 62928 CDS YP_935260.1 119900047 4608117 4116808..4117332 1 NC_008702.1 DcrH:hemerythrin protein, is transmembrane methyl-accepting protein probably involved in bacterial chemotaxis . 32% Hemerythrin. Pfam:PF01814; Hemerythrin; 1.; High confidence in function and specificity; hypothetical protein 4117332 dcrH3 4608117 dcrH3 Azoarcus sp. BH72 hypothetical protein YP_935260.1 4116808 D 62928 CDS YP_935261.1 119900048 4608592 complement(4117384..4117836) 1 NC_008702.1 Hemerythrin family protein, 38% Identity to TrEMBl:Q74G47,35% to TrEMBL; Q73NY7. Has PF01814,Hemerythrin HHE cation binding domain; IPR002063 Hemerythrin; Iteration of the HHE family found it to be related to Hemerythrin. It also demonstrated that what has been described as a single domain in fact consists of two cation binding domains. Members of this family occur all across nature and are involved in a variety of processes. For instance, in Nereis diversicolor P80255 binds Cadmium so as to protect the organism from toxicity ([3]). However Hemerythrin is classically described as Oxygen-binding through two attached Fe2+ ions. And the bacterial Q7WX96 is a regulator of response to NO, which suggests yet another set-up for its metal ligands . In Staphylococcus aureus P72360 has been noted to be important when the organism switches to living in environments with low oxygen concentrations; perhaps this protein acts as an oxygen store or scavenger.; hemerythrin family protein 4117836 4608592 azo3759 Azoarcus sp. BH72 hemerythrin family protein YP_935261.1 4117384 R 62928 CDS YP_935262.1 119900049 4608118 4118075..4119517 1 NC_008702.1 Hypothetical secreted protein. No good homology of the entire protein to the data bank. Has PF06097,Bacterial protein of unknown function (DUF945);IPR010352; This family consists of several hypothetical bacterial proteins of unknown function. No TMHs Siganl peptide present.; hypothetical protein 4119517 4608118 azo3760 Azoarcus sp. BH72 hypothetical protein YP_935262.1 4118075 D 62928 CDS YP_935263.1 119900050 4608119 4119639..4120235 1 NC_008702.1 Conserved hypothetical protein. Homology to ebD78 of Azoarcus sp. EbN1 of 40% (gnl|keqq|eba:ebD78(KEGG)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 4120235 4608119 azo3761 Azoarcus sp. BH72 hypothetical protein YP_935263.1 4119639 D 62928 CDS YP_935264.1 119900051 4608120 4120286..4121464 1 NC_008702.1 Hypothetical patatin-like protein. Homology to all2302 of Anabaena sp. of 20% (trembl|Q8YUN7). Pfam: Patatin. PATATIN MAY HAVE A DUAL ROLE AS A SOMATIC STORAGE PROTEIN AND AS AN ENZYME INVOLVED IN HOST RESISTANCE. THIS TUBER PROTEIN REPRESENTS APPROXIMATELY 40% OF THE TOTAL PROTEIN IN MATURE TUBERS. InterPro: Patatin (IPR002641). no signal peptide. no TMHs; patatin-like protein 4121464 4608120 azo3762 Azoarcus sp. BH72 patatin-like protein YP_935264.1 4120286 D 62928 CDS YP_935265.1 119900052 4608121 4121479..4122924 1 NC_008702.1 Hypothetical protein. no homology to the data bank no domains no signal peptide no TMHs; hypothetical protein 4122924 4608121 azo3763 Azoarcus sp. BH72 hypothetical protein YP_935265.1 4121479 D 62928 CDS YP_935266.1 119900053 4608122 complement(4122934..4123830) 1 NC_008702.1 Hypothetical 21.1 kDa protein in fasciation locus (ORF6). TREMBL:Q8FV26: 50% identity, 65% similarity InterPro:IPR005269; Cons_hypoth730. Pfam:PF03641; Lysine_decarbox L18_bact: ribosomal protein L18 Absence of signal peptide (Signal P predicted) Absence of transmembrane helices (TMHMM predicted); Specificity unclear; hypothetical protein 4123830 4608122 azo3764 Azoarcus sp. BH72 hypothetical protein YP_935266.1 4122934 R 62928 CDS YP_935267.1 119900054 4608123 4124278..4125519 1 NC_008702.1 Probable GTP cyclohydrolase II. TIGRFAM: ribA: GTP cyclohydrolase II; Function unclear; hypothetical protein 4125519 ribA 4608123 ribA Azoarcus sp. BH72 hypothetical protein YP_935267.1 4124278 D 62928 CDS YP_935268.1 119900055 4609720 4125568..4126857 1 NC_008702.1 Conserved hypothetical protein. Homology to blr7238 of B.japonicum of 48% (trembl:Q89E49). No domains predicted. No TMHs. Signal peptide with a probability of 0.570.; hypothetical protein 4126857 4609720 azo3766 Azoarcus sp. BH72 hypothetical protein YP_935268.1 4125568 D 62928 CDS YP_935269.1 119900056 4608124 4126875..4127507 1 NC_008702.1 Uracil phosphoribosyltransferase. (UMP pyrophosphorylase) (UMP diphosphorylase); High confidence in function and specificity; uracil phosphoribosyltransferase 4127507 upp 4608124 upp Azoarcus sp. BH72 uracil phosphoribosyltransferase YP_935269.1 4126875 D 62928 CDS YP_935270.1 119900057 4610068 complement(4127515..4129629) 1 NC_008702.1 Region start changed from 4129741 to 4129603 (-138 bases); putative catalase 4129629 4610068 azo3768 Azoarcus sp. BH72 putative catalase YP_935270.1 4127515 R 62928 CDS YP_935271.1 119900058 4608125 4129784..4131070 1 NC_008702.1 Catalyzes the deamination of cytosine to uracil and ammonia; cytosine deaminase 4131070 codA 4608125 codA Azoarcus sp. BH72 cytosine deaminase YP_935271.1 4129784 D 62928 CDS YP_935272.1 119900059 4608507 4131244..4131738 1 NC_008702.1 conserved hypothetical protein, 43% identity (60% similarity) to TrEMBL; Q8ZML9. TrEMBL;Q8X937(44% identity to Escherichia coli) Has PF04972, Putative phospholipid-binding domain;IPR007055, TAD; This domain is found in a family of osmotic shock protection proteins (e.g. P27291). It is also found in some Secretins and a group of potential haemolysins. Its likely function is attachment to phospholipid membranes. Has SMART;SM00257,LysM, Lysin motif:IPR002482; This domain is about 40 residues long and is found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function.; LysM domain/BON superfamily protein 4131738 4608507 azo3770 Azoarcus sp. BH72 LysM domain/BON superfamily protein YP_935272.1 4131244 D 62928 CDS YP_935273.1 119900060 4608126 4131848..4132270 1 NC_008702.1 Probable Hypothetical protein AQ_1494. TREMBL:Q7NVG8: 46% identity, 64% similarity. InterPro:IPR006684; 4HBcoA_thiostrse. IPR006683; Thioestr_supf. Pfam PF03061; 4HBT TIGRFAMs; TIGR00051; 4HBcoA_thiostrse TIGR00051: conserved hypothetical protein No signal peptide. No transmembrane helices.; Function unclear; thioesterase 4132270 fcbC2 4608126 fcbC2 Azoarcus sp. BH72 thioesterase YP_935273.1 4131848 D 62928 CDS YP_935274.1 119900061 4608736 4132342..4133625 1 NC_008702.1 Hypothetical protein yhiN. TREMBL:Q7NU52: 55% identity, 66% similarity InterPro; IPR001327; FAD_pyr_redox. InterPro; IPR004792; HI0933_like. InterPro; IPR000719; Prot_kinase. InterPro; IPR001100; Pyr_redox InterPro: HI0933-like protein TIGR00275: conserved hypothetical pro Pfam PF03486; HI0933_like Non-secretory protein with no signal peptide (SignalP predicted) No transmembrane helices (TMHMM predicted); Function unclear; hypothetical protein 4133625 4608736 azo3772 Azoarcus sp. BH72 hypothetical protein YP_935274.1 4132342 D 62928 CDS YP_935275.1 119900062 4608127 complement(4133633..4134811) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03002424 of Dechloromonas aromatica of 32% (gi|41723837|ref|ZP_00150727.1|(NBCI ENTREZ)). Pfam: OmpA family. singal peptide. no TMHs; hypothetical protein 4134811 4608127 azo3773 Azoarcus sp. BH72 hypothetical protein YP_935275.1 4133633 R 62928 CDS YP_935276.1 119900063 4608128 complement(4134801..4136297) 1 NC_008702.1 Putative serine/threonine-protein kinase (EC 2.7.11.1). Pfam: Protein kinase domain; Family membership; putative serine/threonine protein kinase 4136297 4608128 azo3774 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_935276.1 4134801 R 62928 CDS YP_935277.1 119900064 4608129 4136601..4137917 1 NC_008702.1 Collagenase and related proteases. Homology to ne1979 of N. europaea of 63% (trembl|Q82TC1). InterPro: Peptidase family U32 (IPR001539). Pfam: Peptidase family U32 no signal peptide no TMHs; Family membership; ORF9; hypothetical protein 4137917 4608129 azo3775 Azoarcus sp. BH72 hypothetical protein YP_935277.1 4136601 D 62928 CDS YP_935278.1 119900065 4608130 complement(4137914..4138504) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to ebA2790 of Azoarcus sp. EbN1 of 31% (gnl|keqq|eba:ebA2790(KEGG)). No domains predicted. no signal peptide. 2 TMHS; Conserved hypothetical protein; hypothetical protein 4138504 4608130 azo3776 Azoarcus sp. BH72 hypothetical protein YP_935278.1 4137914 R 62928 CDS YP_935279.1 119900066 4608131 4138598..4140025 1 NC_008702.1 GGDEF family protein,; Conserved hypothetical protein; diguanylate cyclase 4140025 4608131 azo3777 Azoarcus sp. BH72 diguanylate cyclase YP_935279.1 4138598 D 62928 CDS YP_935280.1 119900067 4608132 4140029..4140520 1 NC_008702.1 Putative streptothricin acetyltransferase. Homology to staT of S. lavenduale (sprot|STA_STRLA). Pfam: Acetyltransferase (GNAT) family. no signal peptide no TMHs; Family membership; putative streptothricin acetyltransferase 4140520 staT 4608132 staT Azoarcus sp. BH72 putative streptothricin acetyltransferase YP_935280.1 4140029 D 62928 CDS YP_935281.1 119900068 4609901 4140608..4142233 1 NC_008702.1 Hypothetical ABC transporter ATP-binding protein ybiT. Probably involved in the transport of Cobalt,branched chain amino acids , or multi drug resistance. Occurence of tub domains, uridine kinase domain, DUF and RecF protein like domains. Signal P present. Central region; Family membership; ABC transporter ATP-binding protein 4142233 ybiT 4609901 ybiT Azoarcus sp. BH72 ABC transporter ATP-binding protein YP_935281.1 4140608 D 62928 CDS YP_935282.1 119900069 4610149 4142403..4142786 1 NC_008702.1 This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The IICD domains contain the sugar binding site and the transmembrane channel; the IIA domain contains the primary phosphorylation site (the donor is phospho-HPr); IIA transfers its phosphoryl group to the IIB domain which finally transfers it to the sugar. 32% EIIA-man.IPR004720; PTSIIB_sorb. Pfam:PF03610; EIIA-man; 1.PF03830; PTSIIB_sorb; 1.; Specificity unclear; putative IIA component of sugar transport PTS system 4142786 ptsL 4610149 ptsL Azoarcus sp. BH72 putative IIA component of sugar transport PTS system YP_935282.1 4142403 D 62928 CDS YP_935283.1 119900070 4609650 4142798..4143067 1 NC_008702.1 Phosphocarrier protein HPr (Histidine-containing protein) (Protein H). This is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III). HPr is common to all PTS (By similarity). 64% HPr_HisP_S.IPR000032; HPr_protein.IPR002114; HPr_SerP_S. IPR005698; PTS_HPr. Pfam:PF00381; PTS-HPr; 1.; High confidence in function and specificity; putative phosphocarrier protein HPr 4143067 ptsH 4609650 ptsH Azoarcus sp. BH72 putative phosphocarrier protein HPr YP_935283.1 4142798 D 62928 CDS YP_935284.1 119900071 4609648 4143071..4144801 1 NC_008702.1 Phosphoenolpyruvate-protein phosphotransferase(Phosphotransferase system enzyme I)(Protein I).This is a component of the phosphoenolpyruvate dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). Enzyme I is common to all PTS. 54% PEP-utilisers_N.IPR008279; PEP_mobile.IPR006318; PEP_P_trans.IPR000121; PEP_utilizers. Pfam:PF05524; PEP-utilisers_N; 1.PF00391; PEP-utilizers; 1.PF02896; PEP-utilizers_C; 1. TIGRFAMs:TIGR01417; PTS_I_fam; 1.; High confidence in function and specificity; putative phosphoenolpyruvate-protein phosphotransferase 4144801 ptsI 4609648 ptsI Azoarcus sp. BH72 putative phosphoenolpyruvate-protein phosphotransferase YP_935284.1 4143071 D 62928 CDS YP_935285.1 119900072 4609996 4145096..4146052 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Daro03001609 of Dechloromonas aromatica of 35% (gi|53730883|ref|ZP_00151781.2|(NBCI ENTREZ)). No domains predicted. Signal peptide present. No TMH reported Present.; Conserved hypothetical protein; hypothetical protein 4146052 4609996 azo3783 Azoarcus sp. BH72 hypothetical protein YP_935285.1 4145096 D 62928 CDS YP_935286.1 119900073 4608133 complement(4146076..4146408) 1 NC_008702.1 Hypothetical secreted protein. no homology of the entire protein to the data bank. no domains predicted. signal peptide. TMH in signal peptide; hypothetical protein 4146408 4608133 azo3784 Azoarcus sp. BH72 hypothetical protein YP_935286.1 4146076 R 62928 CDS YP_935287.1 119900074 4608134 complement(4146462..4146809) 1 NC_008702.1 hypothetical protein 4146809 4608134 azo3785 Azoarcus sp. BH72 hypothetical protein YP_935287.1 4146462 R 62928 CDS YP_935288.1 119900075 4608135 4146921..4148009 1 NC_008702.1 Ferredoxin hydrogenase, small subunit. Homology to hoxK of A. eutrophus of 83% (sprot:MBHS_ALCEU). This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. Tigrfam: hydA: hydrogenase (NiFe) small subunit Pfam: NADH ubiquinone oxidoreductase 20kD signal peptide no TMHs; High confidence in function and specificity; ferredoxin hydrogenase small subunit 4148009 hupS 4608135 hupS Azoarcus sp. BH72 ferredoxin hydrogenase small subunit YP_935288.1 4146921 D 62928 CDS YP_935289.1 119900076 4609053 4148006..4149802 1 NC_008702.1 Probable ferredoxin hydrogenase, large subunit. Homology to hoxG of A. eutrophus of 68% (>sprot|MBHL_ALCEU). This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. Pfam: Nickel dpendent hydrogenase no signal peptide no TMHs; High confidence in function and specificity; ferredoxin hydrogenase large subunit 4149802 hupL 4609053 hupL Azoarcus sp. BH72 ferredoxin hydrogenase large subunit YP_935289.1 4148006 D 62928 CDS YP_935290.1 119900077 4609051 4149947..4150654 1 NC_008702.1 Probable Ni/Fe-hydrogenase, B-type cytochrome subunit. Homology to hupZ of A. chroococcum of 65% (sprot|CYBH_AZOCH) Probable b-type cytochrome. InterPro: Nickel-dependent hydrogenase b-type cytochrome subunit (IPR000516) Pfam: Nickel-dependent hydrogenase b-type cytrochrome subunit no signal peptide 4 TMHs; High confidence in function and specificity; Ni/Fe-hydrogenase, B-type cytochrome subunit 4150654 hupC 4609051 hupC Azoarcus sp. BH72 Ni/Fe-hydrogenase, B-type cytochrome subunit YP_935290.1 4149947 D 62928 CDS YP_935291.1 119900078 4609043 4150663..4151007 1 NC_008702.1 Conserved hypothetical protein. Homology to ne1110 of N. europaea of 35% (trembl|Q82VH8). InterPro: Helix-turn-helix motif (IPR0001387). Pfam: Helix-turn-helix. no signal peptide. no TMHs; hypothetical protein 4151007 4609043 azo3789 Azoarcus sp. BH72 hypothetical protein YP_935291.1 4150663 D 62928 CDS YP_935292.1 119900079 4608136 4151089..4151679 1 NC_008702.1 Conserved hypothetical glutathione peroxidase. Homology to gpwA of P. wisconsinensis of 52% (SWISSPROT:GPWA_PSEWI). Glutathione peroxidase (GSHPx), an enzyme whose principal function is to protect against damage from endogenously-formed hydroxyperoxides,catalyses the reduction of hydroxyperoxides by glutathione. InterPro: Glutathione peroxidase (IPR000889),Type I antifreeze protein (IPR000104). Pfam: Glutathione peroxidase. signal peptide. no TMHs; Family membership; glutathione peroxidase 4151679 4608136 azo3790 Azoarcus sp. BH72 glutathione peroxidase YP_935292.1 4151089 D 62928 CDS YP_935293.1 119900080 4608137 complement(4151699..4153393) 1 NC_008702.1 Conserved hypothetical ATPase. Homology to MCA0262 of Methylococcus capsulatus of 68% (gi|53756248|gb|AAU90539.1|(NBCI ENTREZ)). InterPro: AAA-protein (ATPases associated with various cellular activities) (IPR003959);AAA ATPase superfamily (IPR003593) Pfam: ATPase family associated with various cellular activities (domaine from 365 aa to 540 aa) no signal peptide. no TMHs.; Function unclear; ATPase 4153393 4608137 azo3791 Azoarcus sp. BH72 ATPase YP_935293.1 4151699 R 62928 CDS YP_935294.1 119900081 4608138 4153728..4154408 1 NC_008702.1 Hydrogenase expression/formation protein hupD, 54% identity,(63% simialrity) to SwissProt:Q03004. Aliases: hoxM(SwissProt:P40591), hyaD(E.coli). Has PF01750:Hydrogenase maturation protease;The family consists of hydrogenase maturation proteases. In E. coli HypI the hydrogenase maturation protease is involved in processing of HypE the large subunit of hydrogenases 3, by cleavage of its C-terminal. IPR000671:Peptidase_M52; TIGR00072; hydrog_prot; Signal P reporting SIgnal peptide Present. No TMH present.; High confidence in function and specificity; hydrogenase expression/formation protein hupD 4154408 hupD 4608138 hupD Azoarcus sp. BH72 hydrogenase expression/formation protein hupD YP_935294.1 4153728 D 62928 CDS YP_935295.1 119900082 4609044 4154410..4154721 1 NC_008702.1 Putative Hydrogenase expression/formation protein hupF,43% similarity to TrEMBL; Q6PTB2. No Signal Peptide or TMH reported present. Has PF01455:HupF/HypC family(IPR001109);The large subunit of [NiFe]-hydrogenase,as well as other nickel metalloenzymes, is synthesized as a precursor devoid of the metalloenzyme active site. This precursor then undergoes a complex post-translational maturation process that requires a number of accessory proteins. The hydrogenase expression/formation proteins (HUPF/HYPC) form a family of small proteins that are hydrogenase precursor-specific chaperones required for this maturation process . They are believed to keep the hydrogenase precursor in a conformation accessible for metal incorporation.; High confidence in function and specificity; putative hydrogenase expression/formation protein hupF 4154721 hupF 4609044 hupF Azoarcus sp. BH72 putative hydrogenase expression/formation protein hupF YP_935295.1 4154410 D 62928 CDS YP_935296.1 119900083 4609045 4154718..4155176 1 NC_008702.1 Hydrogenase-1 operon protein HyaE, 50% similarity to TrEMBL;Q8XGC7, 46% similarity to SwissProt;P19931. Alias: hupG Has PF07449:Hydrogenase-1 expression protein HyaE(IPR010893);This family contains bacterial hydrogenase-1 expression proteins approximately 120 residues long. This includes the E. coli protein HyaE, and the homologous proteins HoxO of R. eutropha and HupG of R. leguminosarum. Deletion of the hoxO gene in R. eutropha led to complete loss of the uptake [NiFe] hydrogenase activity, suggesting that it has a critical role in hydrogenase assembly. No signal peptide or TMH reported present.; High confidence in function and specificity; hydrogenase-1 operon protein hyaE 4155176 hupG 4609045 hupG Azoarcus sp. BH72 hydrogenase-1 operon protein hyaE YP_935296.1 4154718 D 62928 CDS YP_935297.1 119900084 4609046 4155192..4156064 1 NC_008702.1 Hydrogenase expression/formation protein hupH, 58% similarity to SwissProt:Q03007. TrEMBL;Q6PTB0,TrEMBL;P95499, SwissProt;P19932. Has PF04809(IPR006894):HupH hydrogenase expression protein, C-terminal conserved region;This family represents a C-terminal conserved region found in these bacterial proteins necessary for hydrogenase synthesis. Their precise function is unknown. No Signal peptide or TMH reported Present.; High confidence in function and specificity; hydrogenase-1 operon protein hupH 4156064 hupH 4609046 hupH Azoarcus sp. BH72 hydrogenase-1 operon protein hupH YP_935297.1 4155192 D 62928 CDS YP_935298.1 119900085 4609047 4156109..4156321 1 NC_008702.1 Probable rubredoxin protein, 61% Identity to SProt;P30778, P31912. Has PF00301, Rubredoxin;Rubredoxin is a low molecular weight iron-containing bacterial protein involved in electron transfer,sometimes replacing ferredoxin as an electron carrier. No signal peptide. No TMHs.; High confidence in function and specificity; rubredoxin protein 4156321 hupI 4609047 hupI Azoarcus sp. BH72 rubredoxin protein YP_935298.1 4156109 D 62928 CDS YP_935299.1 119900086 4609048 4156321..4156887 1 NC_008702.1 Probable Hydrogenase expression/formation protein hupJ, 52% similarity to SwissProtP28152. Alias:hybE No Signal Peptide or TMH reported present. thiM: hydroxyethylthiazole kinase; hydrogenase expression/formation protein 4156887 hupJ 4609048 hupJ Azoarcus sp. BH72 hydrogenase expression/formation protein YP_935299.1 4156321 D 62928 CDS YP_935300.1 119900087 4609049 4156888..4157994 1 NC_008702.1 Putative hydrogenase expression/formation protein HupK. Homology to hupK of R. leguminosarum of 33% (gi|48731|emb|CAA37158.1|(NCBI ENTREZ). No domains predicted. No signal peptide or TMH present., ,; Family membership; putative hydrogenase expression/formation protein HupK 4157994 hupK 4609049 hupK Azoarcus sp. BH72 putative hydrogenase expression/formation protein HupK YP_935300.1 4156888 D 62928 CDS YP_935301.1 119900088 4609050 4157987..4158331 1 NC_008702.1 Hydrogenase nickel incorporation protein hypA. Homology to hypA2 of A. eutrophus of 50% (AAB60888). Probably plays a role in an hydrogenase nickel cofactor insertion step. Pfam: Hydrogenase eypression/synthesis hypA Tigrfam: hypA: hydrogenase expression/formation protein no TMHs; High confidence in function and specificity; hydrogenase nickel inorporation protein hypA 4158331 hypA 4609050 hypA Azoarcus sp. BH72 hydrogenase nickel inorporation protein hypA YP_935301.1 4157987 D 62928 CDS YP_935302.1 119900089 4609058 4158470..4159393 1 NC_008702.1 Probable hydrogenase accessory protein HypB. Homology to hypB of E. coli of 56% (sprot|HYPB_ECOLI). IS REQUIRED FOR THE FORMATION OF HYDROGENASE ISOENZYMES. AFFECTS SOME ASPECT OF THE PROCESSING OF HYDROGENASES and NICKEL INCORPORATION scince HYPB GENE LESIONS CAN BE COMPLEMENTED BY HIGH NICKEL ION CONCENTRATION IN THE MEDIUM. Pfam: HypB/UreG nucleotide-binding domain Tigrfam: hypB: hydrogenase accessory protein Hyp no signal peptide no TMHs; High confidence in function and specificity; hydrogenase nickel incorporation protein HypB 4159393 hypB 4609058 hypB Azoarcus sp. BH72 hydrogenase nickel incorporation protein HypB YP_935302.1 4158470 D 62928 CDS YP_935303.1 119900090 4609059 4159390..4161762 1 NC_008702.1 probable hydrogenase maturation protein HypF. Homology to hypF2 of A. eutrophus of 58% (sprot|HYF2_ALCEU). Involved in the hydrogenase maturation process. InterPro: Hydrogenase maturation protein HypF (IPR00421), SuA5/yciO/yrdC family (IPR004388); Acylphosphatase (IPR001792) Pfam: Acylphosphatase, yrdC domain Tigrfam: hypF: hydrogenase maturation protein H no signal peptide no TMHs; High confidence in function and specificity; hydrogenase maturation protein HypF 4161762 hypF 4609059 hypF Azoarcus sp. BH72 hydrogenase maturation protein HypF YP_935303.1 4159390 D 62928 CDS YP_935304.1 119900091 4609063 4161767..4162006 1 NC_008702.1 Probable hydrogenase assembly chaperon HypC. Homology to hypC of R. leguminosarum of 61% (sprot|HYPC_RHILV). Forms a family of small proteins that are hydrogenase precursor-specific chaperones required for this maturation process. They are believed to keep the hydrogenase precursor in a conformation accessible for metal incorporation. Pfam: HypF/HypC family Tigrfam: hypC_hupF: hydrogenase assembly chaperone no signal peptide no TMHs; Function unclear; hydrogenase assembly chaperon HypC 4162006 hypC 4609063 hypC Azoarcus sp. BH72 hydrogenase assembly chaperon HypC YP_935304.1 4161767 D 62928 CDS YP_935305.1 119900092 4609060 4162003..4163145 1 NC_008702.1 Hydrogenase expression/formation protein hypD. Homology to hypD of A. eutrophus of 71% (sprot|HYPD_ALCEU). HypD is involved in hydrogenase formation. InterPro: Hydrogenase formation hypA (IPR002780) Pfam: Hydrogenase formation hypA family Tigrfam: hypD: hydrogenase expression/formation no signal peptide no TMHs; High confidence in function and specificity; hydrogenase expression/formation protein HypD 4163145 hypD 4609060 hypD Azoarcus sp. BH72 hydrogenase expression/formation protein HypD YP_935305.1 4162003 D 62928 CDS YP_935306.1 119900093 4609061 4163214..4164266 1 NC_008702.1 Hydrogenase expression/formation protein hypE, 64% Identity to SProt;P31905,TrEMBL;Q6NB52(60% Identity),Q83WU6. Has PF00586, AIR synthase related protein, N-terminal domain;IPR000728 AIR_synth; This family includes Hydrogen expression/formation protein, HypE, which may be involved inthe maturation of NifE hydrogenase; AIR synthase and FGAM synthase, which are involved in de novo purine biosynthesis; and selenide, water dikinase, an enzyme which synthesizes selenophosphate from selenide and ATP. PF02769, AIR synthase related protein, C-terminal domain;IPR010918 AIR_synth_C; This entry includes Hydrogen expression/formation protein, HypE, which may be involved in the maturation of NifE hydrogenase; AIR synthase and FGAM synthase, which are involved in de novo purine biosynthesis; and selenide, water dikinase, an enzyme which synthesizes selenophosphate from selenide and ATP.; hydrogenase expression/formation protein hypE 4164266 hypE 4609061 hypE Azoarcus sp. BH72 hydrogenase expression/formation protein hypE YP_935306.1 4163214 D 62928 CDS YP_935307.1 119900094 4609062 4164280..4165737 1 NC_008702.1 Hydrogenase transcriptional regulatory protein,; High confidence in function and specificity; hydrogenase transcriptional regulatory protein 4165737 hoxA 4609062 hoxA Azoarcus sp. BH72 hydrogenase transcriptional regulatory protein YP_935307.1 4164280 D 62928 CDS YP_935308.1 119900095 4609016 complement(4165753..4166049) 1 NC_008702.1 Hypothetical protein, 33% Identity to TrEMBL;Q8XRA3 Has No domains, repeats, motifs or features could be predicted above threshold.; hypothetical protein 4166049 4609016 azo3806 Azoarcus sp. BH72 hypothetical protein YP_935308.1 4165753 R 62928 CDS YP_935309.1 119900096 4608139 4166203..4167198 1 NC_008702.1 Ferredoxin hydrogenase, small subunit. Homology to hoxB of A. hydrogenophilus of 75% (TREMBL:P94154). Hydrogenases catalyse the reversible oxidation of molecular hydrogen and play a vital role in anaerobic metabolism. InterPro: Hydrogenase (NiFe) small subunit HydA (IPR001821); Respiroatory-chain NADH dehydrogenase 20 kD subunit (IPR006137) Tigrfam: hydA: hydrogenase (NiFe) small subunit (hydA) Pfam: NADH ubiquinone oxidoreductase no signal peptide no TMHs; High confidence in function and specificity; ferredoxin hydrogenase small subunit 4167198 hoxB 4608139 hoxB Azoarcus sp. BH72 ferredoxin hydrogenase small subunit YP_935309.1 4166203 D 62928 CDS YP_935310.1 119900097 4609017 4167356..4168807 1 NC_008702.1 Probable ferredoxin hydrogenase, large subunit. Homology to hoxC of A. hydrogenophilus of 59% (trembl|P94155). InterPro: Nickel-dependent hydrogenases large subunit; ferredoxin hydrogenase large subunit 4168807 hoxC 4609017 hoxC Azoarcus sp. BH72 ferredoxin hydrogenase large subunit YP_935310.1 4167356 D 62928 CDS YP_935311.1 119900098 4609018 4168926..4170437 1 NC_008702.1 Conserved hypothetical membrane protein,; Conserved hypothetical protein; hypothetical protein 4170437 4609018 azo3809 Azoarcus sp. BH72 hypothetical protein YP_935311.1 4168926 D 62928 CDS YP_935312.1 119900099 4608140 4170469..4171854 1 NC_008702.1 Putative hydrogen uptake histidine-kinase,ATPbind_ATPase. IPR004358; Bact_sens_pr_C. IPR005467; His_kinase. IPR003661; His_kinA_N. IPR009082; His_kin_homodim. IPR001610; PAC. IPR000700; PAS-assoc_C. IPR000014; PAS_domain. Pfam: PF02518; HATPase_c. PF00512; HisKA. SMART: SM00387; HATPase_c. SM00388; HisKA. SM00086; PAC. SM00091; PAS.; High confidence in function and specificity; hydrogen uptake histidine-kinase 4171854 hoxJ 4608140 hoxJ Azoarcus sp. BH72 hydrogen uptake histidine-kinase YP_935312.1 4170469 D 62928 CDS YP_935313.1 119900100 4609021 complement(4171861..4172355) 1 NC_008702.1 Nitrilotriacetate monooxygenase component B (EC 1.14.13.-) (NTA monooxygenase component B) (NTA-MO B). HYDROXYLATION OF NITRILOTRIACETATE. TREMBL:Q7W4Q0: 46% identity, 65% similarity InterPro: Flavin reductase-like domain Interpro:IPR002563 Pfam:PF01613 No signal peptide No transmembrane helices monomer_idh: isocitrate dehydrogenase NA; High confidence in function and specificity; nitrilotriacetate monooxygenase 4172355 ntaB 4609021 ntaB Azoarcus sp. BH72 nitrilotriacetate monooxygenase YP_935313.1 4171861 R 62928 CDS YP_935314.1 119900101 4609411 4172470..4173570 1 NC_008702.1 A/G-specific adenine glycosylase (EC 3.2.2.-). ADENINE GLYCOSYLASE ACTIVE ON G-A MISPAIRS. MUTY ALSO CORRECTS ERROR-PRONE DNA SYNTHESIS PAST GO LESIONS WHICH ARE DUE TO THE OXIDATIVELY DAMAGED FORM OF GUANINE: 78-DIHYDRO-8- OXOGUANINE.; Specificity unclear; putative A/G-specific adenine glycosylase 4173570 mutY 4609411 mutY Azoarcus sp. BH72 putative A/G-specific adenine glycosylase YP_935314.1 4172470 D 62928 CDS YP_935315.1 119900102 4609298 complement(4173767..4174366) 1 NC_008702.1 Conserved hypothetical ATP-dependent protease La. Homology to rsc0402 of R. solanacearum of 44% (trembl|Q8Y2D4). N-terminal domain of the ATP-dependent protease La (LON), present also in other bacterial ORFs. InterPro: ATP-dependent protease La (LON) domain (IPR003111). Pfam: ATP-dependent protease La (LON) domain. no signal peptide. no TMHs; Conserved hypothetical protein; ATP-dependent protease La 4174366 4609298 azo3813 Azoarcus sp. BH72 ATP-dependent protease La YP_935315.1 4173767 R 62928 CDS YP_935316.1 119900103 4608141 complement(4174401..4175030) 1 NC_008702.1 Probable aromatic acid decarboxylase (EC 4.1.1.-). InterPro: Flavoprotein ubiX: phenylacrylic acid decarboxylase; High confidence in function and specificity; aromatic acid decarboxylase 4175030 ubiX 4608141 ubiX Azoarcus sp. BH72 aromatic acid decarboxylase YP_935316.1 4174401 R 62928 CDS YP_935317.1 119900104 4610062 4175247..4175798 1 NC_008702.1 Probable phasin. Homology to p8 of Sphingomonas sp. A1 of 49% (gi|51773776|dbj|BAD38885.1|(NBCI ENTREZ)). No domains predicted. No TMHs. No signal peptide.,; Function unclear; phasin 4175798 4610062 azo3815 Azoarcus sp. BH72 phasin YP_935317.1 4175247 D 62928 CDS YP_935318.1 119900105 4608142 complement(4175897..4176934) 1 NC_008702.1 Putative AraC family transcriptional regulator,; Family membership; AraC family transcriptional regulator 4176934 4608142 azo3816 Azoarcus sp. BH72 AraC family transcriptional regulator YP_935318.1 4175897 R 62928 CDS YP_935319.1 119900106 4608143 4177099..4178040 1 NC_008702.1 Conserved hypothetical protein. Homology to slr1189 of Synechocystis of 43% (trembl|P74718). Pfam: Homocystein S-transferase. no signal peptide. no TMHs.; hypothetical protein 4178040 4608143 azo3817 Azoarcus sp. BH72 hypothetical protein YP_935319.1 4177099 D 62928 CDS YP_935320.1 119900107 4608144 complement(4178048..4179208) 1 NC_008702.1 In enteric bacteria such as E. coli and Salmonella typhimurium, periplasmic binding proteins are found to participate in the transport of amino acids, sugars and ions. Leucine-specific binding protein are coded by livK and livJ. Similar to sprot|LIVK_SALTY (20%) and to tremblnew|CAE26678 (39%). Pfam (PF01094): Receptor family ligand binding region SignalP reporting Signal peptide.; Specificity unclear; putative leucine-specific binding protein 4179208 livK5 4608144 livK5 Azoarcus sp. BH72 putative leucine-specific binding protein YP_935320.1 4178048 R 62928 CDS YP_935321.1 119900108 4609157 complement(4179274..4180221) 1 NC_008702.1 Putative branched-chain amino acid transporter permease. Homology to livM of E. coli of 32%. Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane. InterPro: Binding-system dependent bacterial transporters (araH livH/limM families) Pfam: Branched-chain amino acid transport system. signal peptide probable 10 TMHs; Specificity unclear; putative branched-chain amino acid transport permease 4180221 livM6 4609157 livM6 Azoarcus sp. BH72 putative branched-chain amino acid transport permease YP_935321.1 4179274 R 62928 CDS YP_935322.1 119900109 4609163 complement(4180301..4181158) 1 NC_008702.1 Branched-chain amino acid transport system typically composed of a periplasmic substrate-binding protein, one or two reciprocally homologous integral inner-membrane proteins and one or two peripheral membrane ATP-binding proteins that couple energy to the active transport system.The integral inner-membrane proteins translocate the substrate across the membrane. Similar to trembl|Q89C65 (46%), to sprot|LIVH_ECOLI (33%) and to sprot|BRAD_PSEAE (30%). Pfam (PF02653): Binding-system dependent bacterial transporters (araH, livH/limM families) TMHMM reporting seven Tmhelix. SignalP reporting Signal peptide.; Specificity unclear; ABC transporter permease 4181158 4609163 azo3820 Azoarcus sp. BH72 ABC transporter permease YP_935322.1 4180301 R 62928 CDS YP_935323.1 119900110 4608145 complement(4181166..4182143) 1 NC_008702.1 2-nitropropane dioxygenase precursor (EC 1.13.11.32) (Nitroalkane oxidase) (2-NPD). SPROT:Q01284: 28% identity, 44% similarity CATALYZES THE OXIDATION OF NITROALKANES TO PRODUCE THE CORRESPONDING CARBONYL COMPOUNDS. IT ACTS ON 2-NITROPROPANE BETTER THAN ON NITROETHANE AND 1-NITROPROPANE AND ANIONIC FORMS OF NITROALKANES ARE MUCH BETTER SUBSTRATES THAN ARE NEUTRAL FORMS. InterPro: 2-nitropropane dioxygenase InterPro; IPR004136; 2nprop_dioxygen. InterPro; IPR003009; FMN_enzyme. Pfam; PF03060; NPD thiE: thiamine-phosphate pyrophosphoryl Non-secretory protein with signal peptide probability:0.004 Absence of TMH's; High confidence in function and specificity; putative 2-nitropropane dioxygenase 4182143 ncd2 4608145 ncd2 Azoarcus sp. BH72 putative 2-nitropropane dioxygenase YP_935323.1 4181166 R 62928 CDS YP_935324.1 119900111 4609347 4182309..4182734 1 NC_008702.1 homopentamer; channel that opens in response to pressure or hypoosmotic shock; large-conductance mechanosensitive channel 4182734 mscL 4609347 mscL Azoarcus sp. BH72 large-conductance mechanosensitive channel YP_935324.1 4182309 D 62928 CDS YP_935325.1 119900112 4609279 complement(4182759..4183517) 1 NC_008702.1 Hypothetical protein ycbL. TREMBL:Q7P1X5-63% identity. Interpro:IPR001279-Blactamase like Pfam-GlyoxylaseII family relationship. Phosphoribosylglycinamide synthetase,Respiratory chain NADH dehydrogenase; KH domain and RTX toxin acyltransferase family relationship. ispD: 4-diphosphocytidyl-2C-methyl-D-ery; High confidence in function and specificity; hypothetical protein 4183517 ycbL 4609279 ycbL Azoarcus sp. BH72 hypothetical protein YP_935325.1 4182759 R 62928 CDS YP_935326.1 119900113 4610154 complement(4183561..4184352) 1 NC_008702.1 Inositol-1(or4)-monophosphatase 3IMPase3)(IMP3)(Inositol monophosphatase 3).Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. 26% Inositol_P. Pfam:PF00459; Inositol_P; 1.; High confidence in function and specificity; putative inositol monophosphatase 4184352 imp 4610154 imp Azoarcus sp. BH72 putative inositol monophosphatase YP_935326.1 4183561 R 62928 CDS YP_935327.1 119900114 4609078 4184498..4185523 1 NC_008702.1 Part of the ABC transporter complex FbpABC involved in ferric import. Similar to the putative periplasmic-binding protein FbpA precursor in A. pleuropneumoniae. 39% SBP_bac_1. Pfam:PF01547; SBP_bac_1; 1. Signal peptide:1 TMHelix:1; Specificity unclear; putative ferric transport system periplasmic-binding protein 4185523 fbpA2 4609078 fbpA2 Azoarcus sp. BH72 putative ferric transport system periplasmic-binding protein YP_935327.1 4184498 D 62928 CDS YP_935328.1 119900115 4608731 4185569..4186576 1 NC_008702.1 Ferric cations import ATP-binding protein fbpC (EC 3.6.3.30).Part of the ABC transporter complex fbpABC (TC 3.A.1.10.1) involved in ferric cations import. Responsible for energy coupling to the transport system. 41% AAA_ATPase. IPR003439; ABC_transporter. Pfam: PF00005;ABC_tran; 1.; Specificity unclear; Fe3+-transporting ATPase 4186576 fbpC2 4608731 fbpC2 Azoarcus sp. BH72 Fe3+-transporting ATPase YP_935328.1 4185569 D 62928 CDS YP_935329.1 119900116 4608734 4186656..4188308 1 NC_008702.1 Region start changed from 4186498 to 4186630 (-132 bases); putative ferrric transport system permease 4188308 4608734 azo3827 Azoarcus sp. BH72 putative ferrric transport system permease YP_935329.1 4186656 D 62928 CDS YP_935330.1 119900117 4608146 4188333..4189109 1 NC_008702.1 Glycerol-3-phosphate regulon repressor. 26% HTH_DeoR. Pfam:PF00455; DeoR; 1. Nucleic acid binding motif, present.; High confidence in function and specificity; putative glycerol-3-phosphate regulon repressor 4189109 glpR 4608146 glpR Azoarcus sp. BH72 putative glycerol-3-phosphate regulon repressor YP_935330.1 4188333 D 62928 CDS YP_935331.1 119900118 4608913 4189133..4190521 1 NC_008702.1 Probable ethanolamine ammonia-lyase (EC 4.3.1.7) large subunit. Homology to eutB of S. typhimurium of 50% (trembl|Q8XUQ9) Catalyzes the deamination of various vicinal amino- alcohols to oxo compounds. no signal peptide no TMHs; High confidence in function and specificity; ethanolamine ammonia-lyase large subunit 4190521 eutB 4608913 eutB Azoarcus sp. BH72 ethanolamine ammonia-lyase large subunit YP_935331.1 4189133 D 62928 CDS YP_935332.1 119900119 4608686 4190532..4191356 1 NC_008702.1 Ethanolamine ammonia-lyase (EC 4.3.1.7) small subunit. Homology to eutC of S. typhimurium of 35% (sprot|EUTC_SALTY) catalyzes the deamination of various vicinal amino- alcohols to oxo compounds. no signal peptide no TMHs; High confidence in function and specificity; putative ethanolamine ammonia-lyase small subunit 4191356 eutC 4608686 eutC Azoarcus sp. BH72 putative ethanolamine ammonia-lyase small subunit YP_935332.1 4190532 D 62928 CDS YP_935333.1 119900120 4608687 4191496..4192371 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to rpa0361 of R. palustris off 67% (tremblnew|CAE25805). no domains predicted .signal peptide. TMH in signal peptide; Conserved hypothetical protein; hypothetical protein 4192371 4608687 azo3831 Azoarcus sp. BH72 hypothetical protein YP_935333.1 4191496 D 62928 CDS YP_935334.1 119900121 4608147 complement(4192520..4193338) 1 NC_008702.1 Putative ABC-type tungstate transport system,permease component.; Function unclear; tungstate ABC transporter permease 4193338 4608147 azo3832 Azoarcus sp. BH72 tungstate ABC transporter permease YP_935334.1 4192520 R 62928 CDS YP_935335.1 119900122 4608148 4193453..4194556 1 NC_008702.1 31% HTH_9; 1. InterPro: IPR003725; ModE:Molybdenum-binding protein N-terminal. ModE are involved in molybdenum transport.These proteins acts both as a repressor and activator of the mod and moa operons,respectively, depending on the properties of the binding site.; High confidence in function and specificity; putative molybdenum-binding protein 4194556 4608148 azo3833 Azoarcus sp. BH72 putative molybdenum-binding protein YP_935335.1 4193453 D 62928 CDS YP_935336.1 119900123 4608149 complement(4194613..4195911) 1 NC_008702.1 Conserved hypothetical protein. Homology to vv12967 of V. vulnificus of 47% (trembl|Q8D8K5). Pfam: DUF1111. no signal peptide. no TMHs; hypothetical protein 4195911 4608149 azo3834 Azoarcus sp. BH72 hypothetical protein YP_935336.1 4194613 R 62928 CDS YP_935337.1 119900124 4608150 complement(4195950..4197269) 1 NC_008702.1 Phosphoglycerate transport regulatory protein pgtC precursor.Required for pgtP expression it may act jointly with the pgtA/pgtB signaling proteins.29% SBP_bac_1: Bacterial extracellular solute-binding protein family 1. Pfam:PF01547; SBP_bac_1; 1. Signal peptide:present.; High confidence in function and specificity; putative regulatory lipoprotein 4197269 pgtC 4608150 pgtC Azoarcus sp. BH72 putative regulatory lipoprotein YP_935337.1 4195950 R 62928 CDS YP_935338.1 119900125 4609534 complement(4197290..4199275) 1 NC_008702.1 Probable Iron-regulated outer membrane TonB-dependent receptor. 35% TonB_receptor.IPR010917; TonB_recept_C.IPR010105; TonB_siderophor. Pfam:PF00593; TonB_dep_Rec; 1. TIGRFAMs:TIGR01783; TonB-siderophor; 1. Signal peptide:present.; Function unclear; TonB-dependent receptor 4199275 4609534 azo3836 Azoarcus sp. BH72 TonB-dependent receptor YP_935338.1 4197290 R 62928 CDS YP_935339.1 119900126 4608151 complement(4199407..4200564) 1 NC_008702.1 80% AAA_ATPase superfamily. IPR003439; ABC_transporter. IPR008995; MOP_like:molybdenum-pterin binding domain.IPR005116; TOBE. Pfam:PF00005; ABC_tran; 1.PF03459; TOBE; 1.; High confidence in function and specificity; putative molybdenum transport system ATP-binding protein 4200564 modC1 4608151 modC1 Azoarcus sp. BH72 putative molybdenum transport system ATP-binding protein YP_935339.1 4199407 R 62928 CDS YP_935340.1 119900127 4609252 complement(4200561..4201241) 1 NC_008702.1 72% BPD_transp.Binding-protein-dependent transport systems inner membrane component. Pfam:PF00528; BPD_transp; 1. TMHelix:6; High confidence in function and specificity; putative molybdenum transport system permease 4201241 modB1 4609252 modB1 Azoarcus sp. BH72 putative molybdenum transport system permease YP_935340.1 4200561 R 62928 CDS YP_935341.1 119900128 4609250 complement(4201261..4202022) 1 NC_008702.1 73% MolP_ABC_transpt.IPR000437; prok_lipoprot_S. IPR006059; SBP_bac_1. Pfam:PF01547; SBP_bac_1; 1. TIGRFAMs:TIGR01256; modA; 1. Signal peptide: present.; High confidence in function and specificity; putative molybdate transport system periplasmic-binding protein 4202022 modA1 4609250 modA1 Azoarcus sp. BH72 putative molybdate transport system periplasmic-binding protein YP_935341.1 4201261 R 62928 CDS YP_935342.1 119900129 4609248 complement(4202077..4202904) 1 NC_008702.1 80% ModE.IPR004606; Mop.IPR008995; MOP_like.IPR005116; TOBE.Molybdenum-binding protein N-terminal. Pfam:PF02573; HTH_9; 1.PF03459; TOBE; 2. TIGRFAMs:TIGR00637; ModE_repress; 1:ModE molybdate transport.TIGR00638; Mop; 2.; High confidence in function and specificity; putative molybdenum transport protein 4202904 modE 4609248 modE Azoarcus sp. BH72 putative molybdenum transport protein YP_935342.1 4202077 R 62928 CDS YP_935343.1 119900130 4609255 4203085..4203513 1 NC_008702.1 80% Mop.IPR008995;MOP_like.IPR005116;TOBE.InterPro: Molybdenum-pterin binding domain. Mop: molybdenum-pterin binding domain Pfam: PF03459; TOBE; 2. TIGRFAMs:TIGR00638; Mop; 2.; High confidence in function and specificity; putative molybdenum-pterin-binding-protein 4203513 modG 4609255 modG Azoarcus sp. BH72 putative molybdenum-pterin-binding-protein YP_935343.1 4203085 D 62928 CDS YP_935344.1 119900131 4609256 4203575..4204612 1 NC_008702.1 39% AAA_ATPase superfamily. IPR003439; ABC_transporter. IPR008995; MOP_like:molybdenum-pterin binding domain.IPR005116; TOBE. Pfam:PF00005; ABC_tran; 1.PF03459; TOBE; 1.; High confidence in function and specificity; putative molybdate transport system ATP-binding protein 4204612 modC2 4609256 modC2 Azoarcus sp. BH72 putative molybdate transport system ATP-binding protein YP_935344.1 4203575 D 62928 CDS YP_935345.1 119900132 4609253 complement(4204593..4205462) 1 NC_008702.1 Putative exported protein.SignalP reporting signal peptice. Most likely cleavage site between pos. 27 and 28: AHA-AP; Function unclear; hypothetical protein 4205462 4609253 azo3843 Azoarcus sp. BH72 hypothetical protein YP_935345.1 4204593 R 62928 CDS YP_935346.1 119900133 4608152 complement(4205489..4207765) 1 NC_008702.1 Region start changed from 4207859 to 4207739 (-120 bases); putative TonB-dependent receptor 4207765 4608152 azo3844 Azoarcus sp. BH72 putative TonB-dependent receptor YP_935346.1 4205489 R 62928 CDS YP_935347.1 119900134 4608153 complement(4208004..4208693) 1 NC_008702.1 36% BPD_transp.Binding-protein-dependent transport systems inner membrane component. Pfam:PF00528; BPD_transp; 1. TMHelix:5 Signal peptide:present; High confidence in function and specificity; putative molybdenum ABC transporter permease 4208693 modB2 4608153 modB2 Azoarcus sp. BH72 putative molybdenum ABC transporter permease YP_935347.1 4208004 R 62928 CDS YP_935348.1 119900135 4609251 complement(4208693..4209478) 1 NC_008702.1 23% MolP_ABC_transpt.IPR000437; prok_lipoprot_S. IPR006059; SBP_bac_1. Pfam:PF01547; SBP_bac_1; 1. TIGRFAMs:TIGR01256; modA; 1. Signal peptide: present.; High confidence in function and specificity; putative molybdate transport system substrate-binding protein 4209478 modA2 4609251 modA2 Azoarcus sp. BH72 putative molybdate transport system substrate-binding protein YP_935348.1 4208693 R 62928 CDS YP_935349.1 119900136 4609249 complement(4209537..4209923) 1 NC_008702.1 conserved hypothetical protein. Homology to Daro03003735 of Dechloromonas aromatica of 35% (gi|41722661|ref|ZP_00149650.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMHs.; hypothetical protein 4209923 4609249 azo3847 Azoarcus sp. BH72 hypothetical protein YP_935349.1 4209537 R 62928 CDS YP_935350.1 119900137 4608154 4210313..4212361 1 NC_008702.1 Function unclear; outer membrane protein 4212361 yhjG 4608154 yhjG Azoarcus sp. BH72 outer membrane protein YP_935350.1 4210313 D 62928 CDS YP_935351.1 119900138 4610183 complement(4212375..4214444) 1 NC_008702.1 GGDEF/EAL/PAS/PAC-domain containing protein; diguanylate cyclase 4214444 4610183 azo3849 Azoarcus sp. BH72 diguanylate cyclase YP_935351.1 4212375 R 62928 CDS YP_935352.1 119900139 4608155 complement(4214513..4216465) 1 NC_008702.1 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide). EXHIBITS SEVERAL CATALYTIC ACTIVITIES INCLUDING ATP-DEPENDENT EXONUCLEASE ATP-STIMULATED ENDONUCLEASE ATP-DEPENDENT UNWINDING AND DNA-DEPENDENT ATPASE ACTIVITIES. STRAND CLEAVAGE OCCURS 5 TO 3 DURING THE UNWINDING OF DUPLEX DNA AT CHI SEQUENCES WHICH LOCALLY STIMULATE RECOMBINATION. InterPro: AAA ATPase superfamily; High confidence in function and specificity; DNA helicase 4216465 recD 4608155 recD Azoarcus sp. BH72 DNA helicase YP_935352.1 4214513 R 62928 CDS YP_935353.1 119900140 4609705 complement(4216462..4220187) 1 NC_008702.1 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) (Exodeoxyribonuclease V 135 KDA polypeptide). REQUIRED FOR EFFICIENT DNA REPAIR; IT CATALYZES THE UNWINDING OF DOUBLE-STRANDED DNA AND THE CLEAVAGE OF SINGLE- STRANDED DNA AND IT STIMULATES LOCAL GENETIC RECOMBINATION. ALL OF THESE ACTIVITIES REQUIRE CONCOMITANT HYDROLYSIS OF ATP.; High confidence in function and specificity; ATP-dependent exoDNAse subunit beta 4220187 recB 4609705 recB Azoarcus sp. BH72 ATP-dependent exoDNAse subunit beta YP_935353.1 4216462 R 62928 CDS YP_935354.1 119900141 4609703 4220373..4222526 1 NC_008702.1 24% TonB_boxC. Pfam:PF00593; TonB_dep_Rec; 1. Signal peptide present.; High confidence in function and specificity; putative TonB-dependent receptor 4222526 irgA2 4609703 irgA2 Azoarcus sp. BH72 putative TonB-dependent receptor YP_935354.1 4220373 D 62928 CDS YP_935355.1 119900142 4609088 4222614..4223768 1 NC_008702.1 Conserved hypothetical protein; Function unclear; hypothetical protein 4223768 4609088 azo3853 Azoarcus sp. BH72 hypothetical protein YP_935355.1 4222614 D 62928 CDS YP_935356.1 119900143 4608156 4223747..4224304 1 NC_008702.1 2-5 RNA ligase (EC 6.5.1.-). Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2-5 phosphodiester linkage.; 2'-5' RNA ligase 4224304 ligT 4608156 ligT Azoarcus sp. BH72 2'-5' RNA ligase YP_935356.1 4223747 D 62928 CDS YP_935357.1 119900144 4609145 4224451..4226037 1 NC_008702.1 Alkaline phosphatase (EC 3.1.3.1) (ALP)is a zinc and magnesium-containing metalloenzyme which hydrolyzes phosphate esters, optimally at high pH.It is found in nearly all living organisms,with the exception of some plants. 30% Alk_phosphtse.Alkaline phosphatase family Pfam:PF00245; alk_phosphatase; 1. Signal peptide present.; High confidence in function and specificity; putative alkaline phosphatase 4226037 4609145 azo3855 Azoarcus sp. BH72 putative alkaline phosphatase YP_935357.1 4224451 D 62928 CDS YP_935358.1 119900145 4608157 4226137..4226919 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to Avin02002740 of Azotobacter vinelandii of 66% (gi|23103854|ref|ZP_00090328.1|(NBCI ENTREZ)). no domains predicted. signal peptide. no TMHS; Conserved hypothetical protein; hypothetical protein 4226919 4608157 azo3856 Azoarcus sp. BH72 hypothetical protein YP_935358.1 4226137 D 62928 CDS YP_935359.1 119900146 4608158 complement(4226936..4227793) 1 NC_008702.1 Function:- Could possibly oxidizes fatty acids using specific components (By similarity). Activity:- (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O. Entry name:- SWISSPROT:PAAG_ECOLI InterPro:- IPR001753; EnCoA_hydrtse. Pfam:- PF00378; ECH; 1. Identities = 65/222 (29%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; Family membership; putative enoyl-CoA hydratase paaG 4227793 paaG6 4608158 paaG6 Azoarcus sp. BH72 putative enoyl-CoA hydratase paaG YP_935359.1 4226936 R 62928 CDS YP_935360.1 119900147 4609477 complement(4227790..4231209) 1 NC_008702.1 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) (Exodeoxyribonuclease V 125 kDa polypeptide). EXHIBITS A WIDE VARIETY OF CATALYTIC ACTIVITIES INCLUDING ATP-DEPENDENT EXONUCLEASE ATP-STIMULATED ENDONUCLEASE ATP-DEPENDENT HELICASE AND DNA-DEPENDENT ATPASE ACTIVITIES.; High confidence in function and specificity; DNA helicase 4231209 recC 4609477 recC Azoarcus sp. BH72 DNA helicase YP_935360.1 4227790 R 62928 CDS YP_935361.1 119900148 4609704 complement(4231262..4232182) 1 NC_008702.1 Putative transcriptional regulator, LysR family,; High confidence in function and specificity; LysR family transcriptional regulator 4232182 yeaT 4609704 yeaT Azoarcus sp. BH72 LysR family transcriptional regulator YP_935361.1 4231262 R 62928 CDS YP_935362.1 119900149 4610167 4232582..4233661 1 NC_008702.1 Tartrate dehydrogenase catalyzes the reduction of tartrate to oxaloglycolate. Similar to sprot|TTUC_ECOLI (75%) and to sprot|TTUC_PSEPU (76%). Pfam (PF00180): Isocitrate and isopropylmalate dehydrogenases; High confidence in function and specificity; tartrate dehydrogenase 4233661 yeaU 4610167 yeaU Azoarcus sp. BH72 tartrate dehydrogenase YP_935362.1 4232582 D 62928 CDS YP_935363.1 119900150 4610168 complement(4233743..4233961) 1 NC_008702.1 Hypothetical protein, 73% identity(87% similarity) to TrEMBL;Q8XU60. No domains, repeats, motifs or features not present.; Function unclear; hypothetical protein 4233961 4610168 azo3861 Azoarcus sp. BH72 hypothetical protein YP_935363.1 4233743 R 62928 CDS YP_935364.1 119900151 4608159 complement(4234040..4234657) 1 NC_008702.1 Conserved hypothetical membrane protein. Homology to rsc2949 of R. solanacearum of 54% (trembl|Q8XV82(SRS)). Tigrfam: TIGR00427: conserved hypothetical protein. Pfam: Uncharacterised protein family. signal peptide. 5 TMHs; hypothetical protein 4234657 4608159 azo3862 Azoarcus sp. BH72 hypothetical protein YP_935364.1 4234040 R 62928 CDS YP_935365.1 119900152 4608160 4234851..4236512 1 NC_008702.1 Conserved hypothetical protein. Homology to ebA2737 of Azoarcus sp. EbN1 of 63% (gnl|keqq|eba:ebA2737(KEGG)). no domains predicted. no signal peptide. no TMHs; hypothetical protein 4236512 4608160 azo3863 Azoarcus sp. BH72 hypothetical protein YP_935365.1 4234851 D 62928 CDS YP_935366.1 119900153 4608161 complement(4236519..4236902) 1 NC_008702.1 Conserved hypothetical cytochrome c family protein. Homology to gsu2513 of G. sulfurreducens of 36% (tremblnew|AAR35886). Pfam: Cytochrome c. singal peptide. no TMHs; Conserved hypothetical protein; cytochrome c family protein 4236902 4608161 azo3864 Azoarcus sp. BH72 cytochrome c family protein YP_935366.1 4236519 R 62928 CDS YP_935367.1 119900154 4608162 complement(4236962..4238632) 1 NC_008702.1 Quinoprotein ethanol dehydrogenase precursor (QEDH). Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol. 32% Bac_PQQ.IPR002372; Bac_PQQ_repeat. Pfam:PF01011; PQQ; 2. Signal peptide:present. SWISSPROT:DHET_GLUOX:O05542.30%; High confidence in function and specificity; putative quinoprotein ethanol dehydrogenase 4238632 exaA5 4608162 exaA5 Azoarcus sp. BH72 putative quinoprotein ethanol dehydrogenase YP_935367.1 4236962 R 62928 CDS YP_935368.1 119900155 4608693 complement(4238668..4239555) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to GOX0515 of Gluconobacter oxydans of 36% (gnl|keqq|gox:GOX0515(KEGG)). no domains predicted. signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 4239555 4608693 azo3866 Azoarcus sp. BH72 hypothetical protein YP_935368.1 4238668 R 62928 CDS YP_935369.1 119900156 4608163 4239989..4240999 1 NC_008702.1 Probable acetoin dehydrogenase, alpha subunit. Homology to acoA of C. magnum of 42% (trembl|Q46142) CATALYZES THE 26-DICHLOROPHENOLINDOPHENOL-DEPENDENT CLEAVAGE OF ACETOIN INTO ACETATE AND ACETALDEHYDE IN VITRO. THE ALPHA SUBUNIT IS PROBABLY THE CATALYTIC SUBUNIT OF THE ENZYME. Pfam: Dehydrogenase E1 component no signal peptide no TMHs; Family membership; acetoin dehydrogenase subunit alpha 4240999 acoA2 4608163 acoA2 Azoarcus sp. BH72 acetoin dehydrogenase subunit alpha YP_935369.1 4239989 D 62928 CDS YP_935370.1 119900157 4608266 4241016..4242053 1 NC_008702.1 Probable acetoin dehydrogenase, beta subunit. Homology to acoB of. A. eutrophus of 48% (sprot|ACOB_ALCEU) Probable TPP-dependent acetoin dehydrogenase, beta subunit. Homology to acoB of A. eutrophus of 41% (sprot|ACOB_ALCEU) Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme. InterPro: Transketolase (IPR005474) Pfam: Transketoase,pyridine binding domain ; Transketolase, C-terminal domain no signal peptide no TMHs; High confidence in function and specificity; acetoin dehydrogenase subunit beta 4242053 acoB2 4608266 acoB2 Azoarcus sp. BH72 acetoin dehydrogenase subunit beta YP_935370.1 4241016 D 62928 CDS YP_935371.1 119900158 4608268 4242264..4242509 1 NC_008702.1 Conserved hypothetical protein. Homology to oadh21d of H. volcanii of 33% (trembl|Q8U4T3). Pfam: Biotin-requiring enzyme. Interpro: Biotin/Lipoyl attachment (IPR000089). no signal peptide. no TMHs.; hypothetical protein 4242509 4608268 azo3869 Azoarcus sp. BH72 hypothetical protein YP_935371.1 4242264 D 62928 CDS YP_935372.1 119900159 4608164 4242513..4243226 1 NC_008702.1 Hypothetical protein. Homology of the entire orf to the C-terminus of dihydrolipoamide acetyltransferase. Pfam: 2-0xo acid dehydrogenase acyltransferase no TMHs no signal peptide; hypothetical protein 4243226 4608164 azo3870 Azoarcus sp. BH72 hypothetical protein YP_935372.1 4242513 D 62928 CDS YP_935373.1 119900160 4608165 4243226..4243996 1 NC_008702.1 Putative lipoate protein ligase, has weak homology with hits. 35% identity with TrEMBL;Q81M24,Q818P0. Has Signal peptide. Has PF03099, Biotin/lipoate A/B protein ligase family;IPR004143 BPL_LipA_LipB; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organism probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.; putative lipoate protein ligase 4243996 4608165 azo3871 Azoarcus sp. BH72 putative lipoate protein ligase YP_935373.1 4243226 D 62928 CDS YP_935374.1 119900161 4608166 4244062..4244643 1 NC_008702.1 Conserved hypothetical secreted protein. Homology blr7490 of B.japonicum of 47% (trembl|Q89DE9(SRS)). No domains predicted .No TMHs. Signal peptide: present; Conserved hypothetical protein; hypothetical protein 4244643 4608166 azo3872 Azoarcus sp. BH72 hypothetical protein YP_935374.1 4244062 D 62928 CDS YP_935375.1 119900162 4608167 4244733..4246307 1 NC_008702.1 Putative glucase dehydrogenase alpha subunit. Homology to gdhalspha of B. cepacia of 31% (trembl|Q8GQE7). Pfam: GMC oxidoreductase no signal peptide no TMHs; Function unclear; putative glucase dehydrogenase subunit alpha 4246307 4608167 azo3873 Azoarcus sp. BH72 putative glucase dehydrogenase subunit alpha YP_935375.1 4244733 D 62928 CDS YP_935376.1 119900163 4608168 4246369..4246983 1 NC_008702.1 Conserved hypothetical secreted protein.; Conserved hypothetical protein; hypothetical protein 4246983 4608168 azo3874 Azoarcus sp. BH72 hypothetical protein YP_935376.1 4246369 D 62928 CDS YP_935377.1 119900164 4608169 4247215..4249182 1 NC_008702.1 Similar to SWISSPROT:P28614 (44% identity); TREMBL:Q9HWN4 (40% identity); TREMBL:Q92X60 (38% identity). Pfam (PF02954): Bacterial regulatory protein,Fis family. Pfam (PF00158): Sigma-54 interaction domain. HTH reporting nucleic acid binding motif.; Family membership; sigma-54-dependent transcriptional regulator 4249182 4608169 azo3875 Azoarcus sp. BH72 sigma-54-dependent transcriptional regulator YP_935377.1 4247215 D 62928 CDS YP_935378.1 119900165 4608170 4249321..4251393 1 NC_008702.1 Rhs family protein,vgrG 38% identity(54% similarity) to TrEMBL;Q747T8. TrEMBL;O52672. Signal Peptide Present. Has PF04524, Protein of unknown function,DUF586;IPR006533, Rhs_Vgr:This family contains a conserved region in several bacterial proteins of unknown function. These sequences represent the Vgr family of proteins,associated with some classes of Rhs elements. This model does not include a large octapeptide repeat region,VGXXXXXX, found in the Vgr of Rhs classes G and E. Has PF06715, Gp5 C-terminal repeat (2 copies);IPR010609,Gp5_C:This repeat composes the C-terminal part of the bacteriophage T4 baseplate protein Gp5. This region of the protein forms a needle like projection from the baseplate that is presumed to puncture the bacterial cell membrane. Structurally three copies of the repeated region trimerise to form a beta solenoid type structure. This family also includes repeats from bacterial Vgr proteins.; hypothetical protein 4251393 4608170 azo3876 Azoarcus sp. BH72 hypothetical protein YP_935378.1 4249321 D 62928 CDS YP_935379.1 119900166 4608171 4251398..4252432 1 NC_008702.1 Conserved hypothetical protein. Homology to GSU3186 of G.sulfurreducens of 36% (tremblnew|AAR36577(SRS)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4252432 4608171 azo3877 Azoarcus sp. BH72 hypothetical protein YP_935379.1 4251398 D 62928 CDS YP_935380.1 119900167 4608172 4252437..4253474 1 NC_008702.1 Region start changed from 4252363 to 4252411 (-48 bases); hypothetical protein 4253474 4608172 azo3878 Azoarcus sp. BH72 hypothetical protein YP_935380.1 4252437 D 62928 CDS YP_935381.1 119900168 4608173 4253497..4254504 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. no homology of the entier protein to the data bank. no domains predicted. no signal peptide. no TMHs; hypothetical protein 4254504 4608173 azo3879 Azoarcus sp. BH72 hypothetical protein YP_935381.1 4253497 D 62928 CDS YP_935382.1 119900169 4608174 4254527..4255069 1 NC_008702.1 Hypothetical protein. Homology to LA1152 of L.interrogans of 28% (trembl:Q8F6Z6). No domains predicted. No signal peptide. No TMHs; hypothetical protein 4255069 4608174 azo3880 Azoarcus sp. BH72 hypothetical protein YP_935382.1 4254527 D 62928 CDS YP_935383.1 119900170 4608175 4255159..4255536 1 NC_008702.1 Conserved hypothetical protein. Homology to GSU3182 of G.sulfurreducens of 42% (tremblnew|AAR36573(SRS)). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4255536 4608175 azo3881 Azoarcus sp. BH72 hypothetical protein YP_935383.1 4255159 D 62928 CDS YP_935384.1 119900171 4608176 4255546..4255974 1 NC_008702.1 Hypothetical protein, 40% similarity to TrEMBL: O86588. No domains, repeats, motifs or features predicted present.; Function unclear; hypothetical protein 4255974 4608176 azo3882 Azoarcus sp. BH72 hypothetical protein YP_935384.1 4255546 D 62928 CDS YP_935385.1 119900172 4608177 4255979..4257190 1 NC_008702.1 Hypothetical protein,35% identity(53% similarity) to TrEMBL;Q9I731. Very Poor homology with hits in the database over the entire length of protein! No Signal peptide or TMH reported Present.; Family membership; hypothetical protein 4257190 4608177 azo3883 Azoarcus sp. BH72 hypothetical protein YP_935385.1 4255979 D 62928 CDS YP_935386.1 119900173 4608178 4257210..4259045 1 NC_008702.1 Hypothetical regulatory protein,; Conserved hypothetical protein; regulatory protein 4259045 4608178 azo3884 Azoarcus sp. BH72 regulatory protein YP_935386.1 4257210 D 62928 CDS YP_935387.1 119900174 4608179 4259097..4259867 1 NC_008702.1 Putative phosphoprotein phosphatase,; Conserved hypothetical protein; putative phosphoprotein phosphatase 4259867 4608179 azo3885 Azoarcus sp. BH72 putative phosphoprotein phosphatase YP_935387.1 4259097 D 62928 CDS YP_935388.1 119900175 4608180 4259941..4260594 1 NC_008702.1 Hypothetical secreted protein. Homology to cv1891 of C. violaceum of 27% (TrEMBL:Q7NWT8). no domains predicted. signal peptide. no TMHs; hypothetical protein 4260594 4608180 azo3886 Azoarcus sp. BH72 hypothetical protein YP_935388.1 4259941 D 62928 CDS YP_935389.1 119900176 4608181 4260635..4261894 1 NC_008702.1 Hypothetical secreted protein. no Homology of the entiere protein to the data bank. no domains predicted. singal peptide. no TMHs; hypothetical protein 4261894 4608181 azo3887 Azoarcus sp. BH72 hypothetical protein YP_935389.1 4260635 D 62928 CDS YP_935390.1 119900177 4608182 complement(4261940..4263946) 1 NC_008702.1 Serine/threonine-protein kinase pknA (EC 2.7.11.1). Probably required for both normal cellular growth and differentiation. Inactivation of pknA leads to colonies that appear light green and rough in the absence of combined nitrogen. Pfam: Protein kinase domain; Family membership; putative serine/threonine kinase 4263946 4608182 azo3888 Azoarcus sp. BH72 putative serine/threonine kinase YP_935390.1 4261940 R 62928 CDS YP_935391.1 119900178 4608183 complement(4264005..4264706) 1 NC_008702.1 Conserved hypothetical protein. Homology to PA0076 of P.aeruginosa of 38% (trembl:Q9I756). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4264706 4608183 azo3889 Azoarcus sp. BH72 hypothetical protein YP_935391.1 4264005 R 62928 CDS YP_935392.1 119900179 4608184 complement(4264709..4268281) 1 NC_008702.1 Conserved hypothetical ImcF-related protein. Homology to BPP0730 of Bordetella parapertussis of 45% (gnl|keqq|bpa:BPP0730(KEGG)). Has Signal Peptide. TMHMM2 reporting 2 TMH's present. Coils2 Program reporting presence of Coiled-Coil. Has PF06761, ImcF-related;IPR009612; This family represents a conserved region within several bacterial proteins that resemble ImcF, which has been proposed to be involved in Vibrio cholerae cell surface reorganisation, resulting in increased adherence to epithelial cells and increased conjugation frequency. Note that many family members are hypothetical proteins. Has PF06744, Protein of unknown function (DUF1215);IPR010623; This family represents a conserved region situated towards the C-terminal end of several hypothetical bacterial proteins of unknown function. A few members resemble the ImcF protein, which has been proposed to be involved in Vibrio cholerae cell surface reorganisation that results in increased adherence to epithelial cells line and increased conjugation frequency.; Conserved hypothetical protein; ImcF-like protein 4268281 imcF 4608184 imcF Azoarcus sp. BH72 ImcF-like protein YP_935392.1 4264709 R 62928 CDS YP_935393.1 119900180 4609077 complement(4268313..4269617) 1 NC_008702.1 Putative outer membrane protein, OmpA family,36% identity(51%similarity) to TrEMBL;Q93IS0.TrEMBL;Q7AXY6(36% identity) TMHMM2 reporting presence of 1 TMH's. Has PF00691, OmpA family;IPR006665, OmpA/MotB:Most of the bacterial outer membrane proteins in this group are porin-like integral membrane proteins (such as ompA) MEDLINE:,but some are small lipid-anchored proteins.It is also found in MotB and related proteins. They are present in the outer membrane of many Gram-negative organisms.; Conserved hypothetical protein; hypothetical protein 4269617 ompA2 4609077 ompA2 Azoarcus sp. BH72 hypothetical protein YP_935393.1 4268313 R 62928 CDS YP_935394.1 119900181 4609444 complement(4269682..4271016) 1 NC_008702.1 Conserved hypothetical protein,40% Identity (57% similarity)to TrEMBL;Q93EC4 Has PF05936, Bacterial protein of unknown function (DUF876);IPR010263;This family consists of a series of hypothetical bacterial sequences of unknown function.; hypothetical protein 4271016 impJ 4609444 impJ Azoarcus sp. BH72 hypothetical protein YP_935394.1 4269682 R 62928 CDS YP_935395.1 119900182 4609079 complement(4271065..4271583) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to PA1666 of P.aeruginosa of 37% (trembl|Q9I359(SRS)). Signal peptide present. No TMH or any other domains/features reported present.; Conserved hypothetical protein; hypothetical protein 4271583 4609079 azo3893 Azoarcus sp. BH72 hypothetical protein YP_935395.1 4271065 R 62928 CDS YP_935396.1 119900183 4608185 4271852..4272913 1 NC_008702.1 Putative cytoplasmic protein,sciA. TrEMBL;Q83SK5 ,Q7AXZ5 Has PF06812, ImpA-related N-terminal;IPR010657,ImpA_N;This family represents a conserved region located towards the N-terminal end of ImpA and related proteins. ImpA is an inner membrane protein, which has been suggested to be involved with proteins that are exported and associated with colony variations in Actinobacillus actinomycetemcomitans. Note that many family members are hypothetical proteins.; Conserved hypothetical protein; putative cytoplasmic protein 4272913 sciA 4608185 sciA Azoarcus sp. BH72 putative cytoplasmic protein YP_935396.1 4271852 D 62928 CDS YP_935397.1 119900184 4609843 4272962..4273471 1 NC_008702.1 Putative cytoplasmic protein,sciH, 71% identity(87% similarity) to TrEMBL;Q7AXZ1. Has PF05591, Protein of unknown function (DUF770);IPR008312,UCP028301;This family consists of several proteins of unknown function from various bacterial species. These proteins are encoded in pathogenic and symbiotic bacteria as part of an operon (part of the SCI genomic island in Salmonella enterica and the imp locus in Rhizobium leguminosarum) implicated in pathogenicity and protein secretion.These proteins are SciH/ImpB.; High confidence in function and specificity; hypothetical protein 4273471 sciH 4609843 sciH Azoarcus sp. BH72 hypothetical protein YP_935397.1 4272962 D 62928 CDS YP_935398.1 119900185 4609848 4273523..4275019 1 NC_008702.1 Putative cytoplasmic protein, sciI, 68% identity(80% similarity) to TrEMBL;Q93IS7. Has PF05943,Protein of unknown function (DUF877);IPR010269;This family consists of a number of uncharacterised bacterial proteins. The function of this family is unknown.; High confidence in function and specificity; hypothetical protein 4275019 sciI 4609848 sciI Azoarcus sp. BH72 hypothetical protein YP_935398.1 4273523 D 62928 CDS YP_935399.1 119900186 4609849 4275148..4275636 1 NC_008702.1 Putative cytoplasmic protein,sciM , 33% identity(50% similarity) to TrEMBL;Q7AXY8. Has PF05638,Protein of unknown function (DUF796);IPR008514;This family consists of several bacterial proteins of unknown function.; High confidence in function and specificity; hypothetical protein 4275636 sciM 4609849 sciM Azoarcus sp. BH72 hypothetical protein YP_935399.1 4275148 D 62928 CDS YP_935400.1 119900187 4609851 4275831..4276337 1 NC_008702.1 Putative cytoplasmic protein,sciK, 33% identity to TrEMBL;Q7AXZ0. Has PF05638, Protein of unknown function (DUF796);This family consists of several bacterial proteins of unknown function. Signal Peptide or TMH absent.; Conserved hypothetical protein; hypothetical protein 4276337 sciK 4609851 sciK Azoarcus sp. BH72 hypothetical protein YP_935400.1 4275831 D 62928 CDS YP_935401.1 119900188 4609850 4276353..4277174 1 NC_008702.1 Putative cytoplasmic protein,sciE, 37% identity(52% similarity) to TrEMBL;Q93IT1. Has PF07024, ImpE protein;This family consists of several bacterial proteins including ImpE (Q93EC9) from Rhizobium leguminosarum. It has been suggested that the imp locus is involved in the secretion to the environment of proteins, including periplasmic RbsB protein, that cause blocking of infection specifically in pea plants.The exact function of this family is unknown.; High confidence in function and specificity; hypothetical protein 4277174 sciE 4609850 sciE Azoarcus sp. BH72 hypothetical protein YP_935401.1 4276353 D 62928 CDS YP_935402.1 119900189 4609847 4277167..4277676 1 NC_008702.1 Putative cytoplasmic protein, sciD,34% identity (50% similarity) to TrEMBL; Q7AXZ4. Has PF07025, Protein of unknown function (DUF1316);IPR010745;This family consists of several hypothetical bacterial proteins of around 150 residues in length. The function of this family is unknown.; Conserved hypothetical protein; hypothetical protein 4277676 sciD 4609847 sciD Azoarcus sp. BH72 hypothetical protein YP_935402.1 4277167 D 62928 CDS YP_935403.1 119900190 4609846 4277775..4279646 1 NC_008702.1 Putative cytoplasmic protein, sciC, 42% identity (57% similarity) to TrEMBL; Q93IT3. Has PF05947, Bacterial protein of unknown function (DUF879);IPR010272 ; This family consists of several hypothetical bacterial proteins of unknown function.; Function unclear; hypothetical protein 4279646 sciC 4609846 sciC Azoarcus sp. BH72 hypothetical protein YP_935403.1 4277775 D 62928 CDS YP_935404.1 119900191 4609845 4279622..4280665 1 NC_008702.1 Putative cytoplasmic protein, sciB. TrEMBl;Q93IT4(36% identity) Has PF06996;Protein of unknown function (DUF1305);IPR010732 ;This family consists of several hypothetical bacterial proteins of around 300 residues in length. The function of this family is unknown although one member (Q93IT4) from Salmonella enterica is thought to be involved in virulence.; Conserved hypothetical protein; hypothetical protein 4280665 sciB 4609845 sciB Azoarcus sp. BH72 hypothetical protein YP_935404.1 4279622 D 62928 CDS YP_935405.1 119900192 4609844 4280714..4283434 1 NC_008702.1 Putative ATP-dependent Clp protease, ATP-binding subunit ClpB. Homology to clpB of E. coli of 38% (sprot|CLPB_ECOLI). The protein is thought to be subunits of ATP-dependent proteases which act as chaperones to target the proteases to substrates. Pfam: ATPsae family associated with various cellular activites no signal peptide no TMHs; High confidence in function and specificity; putative ATP-dependent Clp protease, ATP-binding subunit ClpB 4283434 clpB2 4609844 clpB2 Azoarcus sp. BH72 putative ATP-dependent Clp protease, ATP-binding subunit ClpB YP_935405.1 4280714 D 62928 CDS YP_935406.1 119900193 4608488 4283535..4283924 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology of the entire protein to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 4283924 4608488 azo3904 Azoarcus sp. BH72 hypothetical protein YP_935406.1 4283535 D 62928 CDS YP_935407.1 119900194 4608186 4283936..4285093 1 NC_008702.1 Entry name:- TREMBL:Q7MFV5 Identities = 112/345 (32%), InterPro:- IPR002921; Lipase_3. Pfam:- PF01764; Lipase_3; 1. Prediction: Non-secretory protein Signal peptide probability: 0.074 Number of predicted TMHs: 0; Family membership; putative lipase family protein 4285093 4608186 azo3905 Azoarcus sp. BH72 putative lipase family protein YP_935407.1 4283936 D 62928 CDS YP_935408.1 119900195 4608187 4285131..4286267 1 NC_008702.1 Hypothetical protein. No homology over the entire length with the data base. Pfam: Phosphatidylinositol-specific phospholipase C, X domain. Interpro: Phosphatidylinositol-specific phospholipase C, X region (IPR000909). No TMHs. No signal peptide.; hypothetical protein 4286267 4608187 azo3906 Azoarcus sp. BH72 hypothetical protein YP_935408.1 4285131 D 62928 CDS YP_935409.1 119900196 4608188 4286286..4287635 1 NC_008702.1 Conserved hypothetical protein. Homology to xac4125 of X. axonopodis of 42% (trembl|Q8PF60). Domain structure: 5 x TRP, 50 aa - 83 aa; 84 aa - 117 aa; 118 aa - 151 aa; 152 aa - 182 aa; 186 aa - 219 aa. InterPro: TPR repeat (IPR001440); SAM (and some other nucleotide) binding motif (IPR000051). Pfam: TPR daomian. no signal peptide. no TMHs; hypothetical protein 4287635 4608188 azo3907 Azoarcus sp. BH72 hypothetical protein YP_935409.1 4286286 D 62928 CDS YP_935410.1 119900197 4608189 complement(4287636..4289066) 1 NC_008702.1 PutativeSerine/threonine-protein kinase (EC 2.7.11.1) (NimA-related protein kinase 4) (Serine/threonine protein kinase 2). Seems to act exclusively upon threonine residues. Pfam: Protein kinase domain; Family membership; putative serine/threonine protein kinase 4289066 4608189 azo3908 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_935410.1 4287636 R 62928 CDS YP_935411.1 119900198 4608190 complement(4289077..4289949) 1 NC_008702.1 Probable sensor protein,; High confidence in function and specificity; sensor protein 4289949 mnsA 4608190 mnsA Azoarcus sp. BH72 sensor protein YP_935411.1 4289077 R 62928 CDS YP_935412.1 119900199 4609233 complement(4290002..4290928) 1 NC_008702.1 Hypothetical secreted protein no homology of the entire protein to the data bank no domains predicted signal peptide no TMHs; hypothetical protein 4290928 4609233 azo3910 Azoarcus sp. BH72 hypothetical protein YP_935412.1 4290002 R 62928 CDS YP_935413.1 119900200 4608191 4291053..4292258 1 NC_008702.1 FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP; 3-oxoacyl-ACP synthase 4292258 fabF2 4608191 fabF2 Azoarcus sp. BH72 3-oxoacyl-ACP synthase YP_935413.1 4291053 D 62928 CDS YP_935414.1 119900201 4608710 4292263..4293090 1 NC_008702.1 Conserved hypothetical protein. Homology to RSc0426 of R.solanacearum of 51% (trembl:Q8Y2B0). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 4293090 4608710 azo3912 Azoarcus sp. BH72 hypothetical protein YP_935414.1 4292263 D 62928 CDS YP_935415.1 119900202 4608192 4293087..4293596 1 NC_008702.1 Entry name:- TREMBL:Q8Y2B1 Prim. accession # Q8Y2B1 Identities = 64/116 (55%) Prediction: Non-secretory protein Signal peptide probability: 0.000 Number of predicted TMHs: 0; hypothetical protein 4293596 4608192 azo3913 Azoarcus sp. BH72 hypothetical protein YP_935415.1 4293087 D 62928 CDS YP_935416.1 119900203 4608193 4293607..4294332 1 NC_008702.1 Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis; 3-ketoacyl-ACP reductase 4294332 fabG 4608193 fabG Azoarcus sp. BH72 3-ketoacyl-ACP reductase YP_935416.1 4293607 D 62928 CDS YP_935417.1 119900204 4608712 4294332..4295636 1 NC_008702.1 23% K_eff.IPR006153; Na_H_porter. Pfam:PF00999; Na_H_Exchanger; 1. TIGRFAMs:TIGR00932; 2a37; 1. TMHelix: 11.; High confidence in function and specificity; putative Na/H(+) antiporter 4295636 4608712 azo3915 Azoarcus sp. BH72 putative Na/H(+) antiporter YP_935417.1 4294332 D 62928 CDS YP_935418.1 119900205 4608194 4295751..4296674 1 NC_008702.1 catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis; N-acetyl-gamma-glutamyl-phosphate reductase 4296674 argC 4608194 argC Azoarcus sp. BH72 N-acetyl-gamma-glutamyl-phosphate reductase YP_935418.1 4295751 D 62928 CDS YP_935419.1 119900206 4608333 complement(4296699..4297466) 1 NC_008702.1 Specificity unclear; glycosyltransferase 4297466 4608333 azo3917 Azoarcus sp. BH72 glycosyltransferase YP_935419.1 4296699 R 62928 CDS YP_935420.1 119900207 4608195 complement(4297495..4298283) 1 NC_008702.1 Putative phospholipid biosynthesis acyltransferase,30% Identity to TrEMBL;Q8FJ00.Q8X6N6 Has Signal Peptide. Has SMART;SM00563, PlsC, Phosphate acyltransferases;Function in phospholipid biosynthesis and have either glycerolphosphate, 1-acylglycerolphosphate, or 2-acylglycerolphosphoethanolamine acyltransferase activities. Tafazzin, the product of the gene mutated in patients with Barth syndrome, is a member of this family.; putative phospholipid biosynthesis acyltransferase 4298283 4608195 azo3918 Azoarcus sp. BH72 putative phospholipid biosynthesis acyltransferase YP_935420.1 4297495 R 62928 CDS YP_935421.1 119900208 4608196 complement(4298304..4299155) 1 NC_008702.1 MltA-interacting protein precursor. MAY SERVE AS A SCAFFOLD PROTEIN REQUIRED FOR THE FORMATION OF A COMPLEX WITH MRCB/PONB AND MLTA THIS COMPLEX COULD PLAY A ROLE IN ENLARGEMENT AND SEPTATION OF THE MUREIN SACCULUS.; High confidence in function and specificity; MltA-interacting protein 4299155 mipA 4608196 mipA Azoarcus sp. BH72 MltA-interacting protein YP_935421.1 4298304 R 62928 CDS YP_935422.1 119900209 4609225 complement(4299152..4300162) 1 NC_008702.1 Conserved Hypothetical protein, Most Hits in the data base suggest to Pteridine-dependent deoxygenase like protein,TrEMBL;Q8P3Q5. Has PF01042 Endoribonuclease L-PSP,(IPR006175:Endoribon_LPSP);This domain is found in endoribonuclease, that is active on single-stranded mRNA and inhibits protein synthesis by cleavage of mRNA. Previously it was thought to inhibit protein synthesis initiation.This endoribonuclease may also be involved in the regulation of purine biosynthesis . No Signal peptide or TMH present!; Family membership; hypothetical protein 4300162 4609225 azo3920 Azoarcus sp. BH72 hypothetical protein YP_935422.1 4299152 R 62928 CDS YP_935423.1 119900210 4608197 complement(4300159..4301538) 1 NC_008702.1 Conserved hypothetical protein. Homology to xcc4013 of X. campestris of 46% (trembl|Q8P3Q6). InterPro: Monooxygenases FAD binding domain (IPR002938); NAD binding site (IPR000205) Pfam: FAD binding domain; Monooxygenase FAD binding domain. no signal peptide. no TMHs; hypothetical protein 4301538 4608197 azo3921 Azoarcus sp. BH72 hypothetical protein YP_935423.1 4300159 R 62928 CDS YP_935424.1 119900211 4608198 complement(4301535..4302155) 1 NC_008702.1 Conserved hypothetical secred protein. Homology to xac4267 of X. axonopodis of 41% (trembl|Q8PES3). no domains predicted .signal peptide. no TMHs; Conserved hypothetical protein; hypothetical protein 4302155 4608198 azo3922 Azoarcus sp. BH72 hypothetical protein YP_935424.1 4301535 R 62928 CDS YP_935425.1 119900212 4608199 complement(4302273..4302821) 1 NC_008702.1 Probable leucine-responsive regulatory protein. Mediates a global response to leucine. Exogenous leucine affects the expression of a number of different opreons; Lrp mediates this effect for at least some of these operons. For example it is regulator of the branched-chain amino acid transport genes. Similar to SWISSPROT: sprot|LRP_ECOLI (49% Escherichia coli, leucine-responsive regulatory protein Lrp) InterPro: IPR000485 HTH_AsnC_lrp. Pfam: PF01037 AsnC family. HTH reporting nucleic acid binding motif.; High confidence in function and specificity; leucine-responsive regulatory protein 4302821 lrp2 4608199 lrp2 Azoarcus sp. BH72 leucine-responsive regulatory protein YP_935425.1 4302273 R 62928 CDS YP_935426.1 119900213 4609181 4302926..4304239 1 NC_008702.1 catalyzes the oxidative deamination of D-amino acids; D-amino acid dehydrogenase small subunit 4304239 dadA2 4609181 dadA2 Azoarcus sp. BH72 D-amino acid dehydrogenase small subunit YP_935426.1 4302926 D 62928 CDS YP_935427.1 119900214 4608579 4304301..4305362 1 NC_008702.1 Alanine racemase catabolic (EC 5.1.1.1). Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by dadA (By similarity). alr: alanine racemase; High confidence in function and specificity; alanine racemase 4305362 alr 4608579 alr Azoarcus sp. BH72 alanine racemase YP_935427.1 4304301 D 62928 CDS YP_935428.1 119900215 4608306 complement(4305381..4305590) 1 NC_008702.1 conserved hypothetical protein. Homology to Daro03003529 of Dechloromonas aromatica of 39% (gi|41722979|ref|ZP_00149945.1|(NBCI ENTREZ)). no domains predicted. no signal peptide. no TMH; hypothetical protein 4305590 4608306 azo3926 Azoarcus sp. BH72 hypothetical protein YP_935428.1 4305381 R 62928 CDS YP_935429.1 119900216 4608200 complement(4305599..4306858) 1 NC_008702.1 Conserved hyppthetical cytochrome c-type biogenesis protein CycH. Homology to cycH of Azoarcus sp. EbN1 of 50% (gnl|keqq|eba:ebA3526(KEGG)). REQUIRED FOR THE BIOGENESIS OF C-TYPE CYTOCHROMES. POSSIBLE SUBUNIT OF A HEME LYASE (BY SIMILARITY). signal peptide. 1 TMHs; Conserved hypothetical protein; cytochrome c-type biogenesis protein CycH 4306858 cycH 4608200 cycH Azoarcus sp. BH72 cytochrome c-type biogenesis protein CycH YP_935429.1 4305599 R 62928 CDS YP_935430.1 119900217 4608550 complement(4306855..4307364) 1 NC_008702.1 Probable Cytochrome c-type biogenesis protein cycL. Homology to cycL of S. meliloti of 44% (sprot|CCMH_RHIME). REQUIRED FOR THE BIOGENESIS OF C-TYPE CYTOCHROMES. POSSIBLE SUBUNIT OF A HEME LYASE. signal peptide probable 2 TMHs; Family membership; cytochrome C-type biogenesis protein CycL 4307364 cycL 4608550 cycL Azoarcus sp. BH72 cytochrome C-type biogenesis protein CycL YP_935430.1 4306855 R 62928 CDS YP_935431.1 119900218 4608551 complement(4307381..4307974) 1 NC_008702.1 Probable thiol disulfide interchange protein. Involved in disulfide bond formation. Catalyzes a late reductive step in the assembly of periplasmic c-type cytochromes probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme lyase (By similarity). InterPro: Periplasmic protein thiol:disulfide oxidoreductase DsbE (IPR004799) Interpro: Thioredoxin (IPR006662) Tigrfam: dsbE: periplasmic protein thiol:disulfide axidoreductase, DsbE superfamily signal peptide Probable 1 TMH; Family membership; protein disulfide-isomerase 4307974 ccmG 4608551 ccmG Azoarcus sp. BH72 protein disulfide-isomerase YP_935431.1 4307381 R 62928 CDS YP_935432.1 119900219 4608450 complement(4307994..4309970) 1 NC_008702.1 Cytochrome c-type biogenesis protein ccmF. Homology to ccmF of E. coli of 63% (sprot|CCMF_ECOLI). REQUIRED FOR THE BIOGENESIS OF C-TYPE CYTOCHROMES. POSSIBLE SUBUNIT OF A HEME LYASE. Tigrfam: nerF: cytochrome c-type biogenesis protein ccmF Pfam: Cytochrome C assembly protein probably signal peptide probably 15 THMs; High confidence in function and specificity; cytochrome c-type biogenesis protein CcmF 4309970 ccmF 4608450 ccmF Azoarcus sp. BH72 cytochrome c-type biogenesis protein CcmF YP_935432.1 4307994 R 62928 CDS YP_935433.1 119900220 4608449 complement(4309967..4310413) 1 NC_008702.1 CycJ; periplasmic heme chaperone that binds heme transiently via a histidine residue and delivers it to newly synthesized and exported c-type cytochromes; requires the ATP hydrolysis activity of the CcmA protein in order to transfer the heme to the apocytochrome; part of the cytochrome c maturation system; periplasmic protein anchored to the inner membrane; cytochrome c-type biogenesis protein CcmE 4310413 ccmE 4608449 ccmE Azoarcus sp. BH72 cytochrome c-type biogenesis protein CcmE YP_935433.1 4309967 R 62928 CDS YP_935434.1 119900221 4608448 complement(4310410..4310631) 1 NC_008702.1 Conserved hypothetical heme exporter protein D. Homology to ccmD of Azoarcus EBN1 of 49% (trembl:Q5P3K7). Pfam: Heme exporter protein D . The CcmD protein is part of a C-type cytochrome biogenesis operon. The exact function of this protein is uncertain. It has been proposed that CcmC, CcmD and CcmE interact directly with each other, establishing a cytoplasm to periplasm haem delivery pathway for cytochrome c maturation. This protein is found fused to CcmE in P52224. These proteins contain a predicted transmembrane helix. No signal peptide. 1 TMHs; Conserved hypothetical protein; heme exporter protein D 4310631 ccmD 4608448 ccmD Azoarcus sp. BH72 heme exporter protein D YP_935434.1 4310410 R 62928 CDS YP_935435.1 119900222 4608447 complement(4310631..4311407) 1 NC_008702.1 Probable heme exporter protein C (Cytochrome c-type biogenesis protein ccmC). Homology to ccmC of E. coli of 43% (sprot|CCMC_ECOLI). Required for the export of heme to the periplasm for the biogenesis of C-type cytochromes. Pfam: Cytrochrome C assembly protein no signal peptide probable 6 TMHs; High confidence in function and specificity; heme exporter protein C 4311407 ccmC 4608447 ccmC Azoarcus sp. BH72 heme exporter protein C YP_935435.1 4310631 R 62928 CDS YP_935436.1 119900223 4608446 complement(4311404..4311499) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica no homology to the data bank no domains predicted no signal peptide no TMHs; hypothetical protein 4311499 4608446 azo3934 Azoarcus sp. BH72 hypothetical protein YP_935436.1 4311404 R 62928 CDS YP_935437.1 119900224 4608201 complement(4311496..4312164) 1 NC_008702.1 Probable heme exporter protein B (Cytochrome c-type biogenesis protein ccmB). Homology to ccmB (sprot|CCMB_ECOLI) of E. coli of 49%. Required for the export of heme to the periplasm for the biogenesis of C-type cytochromes (By similarity). no signal peptide probable 6 TMHs; High confidence in function and specificity; heme exporter protein B 4312164 ccmB 4608201 ccmB Azoarcus sp. BH72 heme exporter protein B YP_935437.1 4311496 R 62928 CDS YP_935438.1 119900225 4608445 complement(4312158..4312784) 1 NC_008702.1 ATP-binding protein; required for proper cytochrome c maturation; cytochrome c biogenesis protein CcmA 4312784 ccmA 4608445 ccmA Azoarcus sp. BH72 cytochrome c biogenesis protein CcmA YP_935438.1 4312158 R 62928 CDS YP_935439.1 119900226 4608444 4313372..4315009 1 NC_008702.1 Putative aerotaxis receptor, sensory_box TMHMM reporting 2 transmembrane helices. HTH reporting nucleic acid binding motif. Aerotaxis receptor. SIGNAL TRANSDUCER FOR AEROTAXIS. THE AEROTACTIC RESPONSES IS THE ACCUMULATION OF CELLS AROUND AIR BUBBLES. THE NATURE OF THE SENSORY STIMULUS DETECTED BY THIS PROTEIN IS THE PROTON MOTIVE FORCE OR CELLULAR REDOX STATE. IT USES A FAD PROSTHETIC GROUP AS A REDOX SENSOR TO MONITOR OXYGEN LEVELS.; Specificity unclear; putative aerotaxis receptor 4315009 4608444 azo3937 Azoarcus sp. BH72 putative aerotaxis receptor YP_935439.1 4313372 D 62928 CDS YP_935440.1 119900227 4608202 complement(4315202..4315840) 1 NC_008702.1 Probable cytochrome c-type protein NapC. Homology to napC of R. sphaeroides of 57% (sprot|NAPC_RHOSH). MEDIATES ELECTRON FLOW FROM QUINONES TO THE NAPAB COMPLEX. no signal peptide probable 1 TMHs; High confidence in function and specificity; cytochrome c-type protein NapC 4315840 napC2 4608202 napC2 Azoarcus sp. BH72 cytochrome c-type protein NapC YP_935440.1 4315202 R 62928 CDS YP_935441.1 119900228 4609333 complement(4315856..4316308) 1 NC_008702.1 Probable diheme cytochrome c. Homology to napB of A. eutrophus of 47% (sprot|NAPB_ALCEU). SMALL SUBUNIT OF THE PERIPLASMIC NITRATE REDUCTASE (NAP). ONLY EXPRESSED AT HIGH LEVELS DURING AEROBIC GROWTH. NAPAB COMPLEX RECEIVES ELECTRONS FROM THE MEMBRANE-ANCHORED TETRAHEME NAPC PROTEIN THUS ALLOWING ELECTRON FLOW BETWEEN MEMBRANE AND PERIPLASM. ESSENTIAL FUNCTION FOR NITRATE ASSIMILATION AND MAY HAVE A ROLE IN ANAEROBIC METABOLISM. signal peptide no TMHs; High confidence in function and specificity; diheme cytochrome c 4316308 napB2 4609333 napB2 Azoarcus sp. BH72 diheme cytochrome c YP_935441.1 4315856 R 62928 CDS YP_935442.1 119900229 4609331 complement(4316305..4317300) 1 NC_008702.1 part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA; quinol dehydrogenase membrane protein 4317300 napH 4609331 napH Azoarcus sp. BH72 quinol dehydrogenase membrane protein YP_935442.1 4316305 R 62928 CDS YP_935443.1 119900230 4609339 complement(4317297..4318157) 1 NC_008702.1 part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA; secreted by twin arginine translocation pathway; quinol dehydrogenase periplasmic protein 4318157 napG 4609339 napG Azoarcus sp. BH72 quinol dehydrogenase periplasmic protein YP_935443.1 4317297 R 62928 CDS YP_935444.1 119900231 4609338 complement(4318452..4320995) 1 NC_008702.1 periplasmic; catalytic subunit; with NapBC catalyzes the reduction of nitrate to nitrite; NapAB receives electrons from NapC; nitrate reductase catalytic subunit 4320995 napA2 4609338 napA2 Azoarcus sp. BH72 nitrate reductase catalytic subunit YP_935444.1 4318452 R 62928 CDS YP_935445.1 119900232 4609329 complement(4320997..4321251) 1 NC_008702.1 Probable NapD protein, 38% Identity to TrEMBL;Q6LTW0, Has PF03927, NapD protein;IPR005623;Uncharacterized protein involved in formation of periplasmic nitrate reductase.; Family membership; periplasmic nitrate reductase subunit NapD 4321251 napD2 4609329 napD2 Azoarcus sp. BH72 periplasmic nitrate reductase subunit NapD YP_935445.1 4320997 R 62928 CDS YP_935446.1 119900233 4609335 complement(4321248..4321751) 1 NC_008702.1 Probabel ferredoxin-type protein napF. Homology to napF of E. coli of 43% (sprot|NAPF_ECOLI). Involved in electron transfer ina a wide variety of metabolic reactions. InterPro: Ferredoxin-type protein NapF (IPR004496); 4Fe-4S ferredoxin, iron-sulfur binding domain (IPR001450) Tigrfam: napF: ferredoxin-type protein NapF Pfam: 4Fe-4S binding domain no signal peptide no TMHs; High confidence in function and specificity; ferredoxin-type protein NapF 4321751 napF 4609335 napF Azoarcus sp. BH72 ferredoxin-type protein NapF YP_935446.1 4321248 R 62928 CDS YP_935447.1 119900234 4609337 complement(4321945..4325139) 1 NC_008702.1 Putative hybrid sensor and regulator protein,; Specificity unclear; putative hybrid sensor and regulator protein 4325139 4609337 azo3945 Azoarcus sp. BH72 putative hybrid sensor and regulator protein YP_935447.1 4321945 R 62928 CDS YP_935448.1 119900235 4608203 4325235..4325588 1 NC_008702.1 Putative transcriptional regulator,; Function unclear; putative two component transcriptional regulator 4325588 4608203 azo3946 Azoarcus sp. BH72 putative two component transcriptional regulator YP_935448.1 4325235 D 62928 CDS YP_935449.1 119900236 4608204 complement(4325551..4328109) 1 NC_008702.1 Putative two component sensor protein,; Function unclear; putative two component sensor protein 4328109 4608204 azo3947 Azoarcus sp. BH72 putative two component sensor protein YP_935449.1 4325551 R 62928 CDS YP_935450.1 119900237 4608205 complement(4328357..4329781) 1 NC_008702.1 Excinuclease cho (EC 3.1.25.-) (Endonuclease cho) (UvrC homolog protein). Incises the DNA at the 3 side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5 site of a lesion. When a lesion remains because uvrC is not able to induce the 3 incision cho incises the DNA. Then uvrC makes the 5 incision. The combined action of cho and uvrC broadens the substrate range of nucleotide excision repair (By similarity).; Specificity unclear; putative DNA-directed polymerase III 4329781 4608205 azo3948 Azoarcus sp. BH72 putative DNA-directed polymerase III YP_935450.1 4328357 R 62928 CDS YP_935451.1 119900238 4608206 complement(4329784..4330548) 1 NC_008702.1 Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (EC 2.1.1.-). Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-14-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-14-benzoquinol (DMQH2) (By similarity). InterPro: ubiE/COQ5 methyltransferase rrmJ: ribosomal RNA large subunit methy; High confidence in function and specificity; ubiquinone/menaquinone biosynthesis methyltransferase 4330548 ubiE2 4608206 ubiE2 Azoarcus sp. BH72 ubiquinone/menaquinone biosynthesis methyltransferase YP_935451.1 4329784 R 62928 CDS YP_935452.1 119900239 4610059 complement(4330672..4330863) 1 NC_008702.1 Conserved hypothetical protein. Homology to RS02765 of R.solanacearum of 43% (trembl:Q8XWT5). No domains predicted. No TMHs. No signal peptide.; hypothetical protein 4330863 4610059 azo3950 Azoarcus sp. BH72 hypothetical protein YP_935452.1 4330672 R 62928 CDS YP_935453.1 119900240 4608207 complement(4330962..4333160) 1 NC_008702.1 Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase,; High confidence in function and specificity; guanosine-3',5'-bis(diphosphate) 3'-diphosphatase 4333160 spoT 4608207 spoT Azoarcus sp. BH72 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase YP_935453.1 4330962 R 62928 CDS YP_935454.1 119900241 4609889 complement(4333183..4333398) 1 NC_008702.1 Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits; DNA-directed RNA polymerase subunit omega 4333398 rpoZ 4609889 rpoZ Azoarcus sp. BH72 DNA-directed RNA polymerase subunit omega YP_935454.1 4333183 R 62928 CDS YP_935455.1 119900242 4609804 complement(4333401..4334015) 1 NC_008702.1 Essential for recycling GMP and indirectly, cGMP; guanylate kinase 4334015 gmk 4609804 gmk Azoarcus sp. BH72 guanylate kinase YP_935455.1 4333401 R 62928 CDS YP_935456.1 119900243 4608928 complement(4334206..4334748) 1 NC_008702.1 Similar to TREMBL:Q9HXG0 (60% identity); TREMBL:Q7NRZ4 (45% identity); TREMBL:Q88GB8 (43% identity). InterPro (IPR000868): Isochorismatase hydrolase. Pfam (PF00857): Isochorismatase family.; Specificity unclear; isochorismatase family protein 4334748 4608928 azo3954 Azoarcus sp. BH72 isochorismatase family protein YP_935456.1 4334206 R 62928 CDS YP_935457.1 119900244 4608208 4335032..4335799 1 NC_008702.1 Conserved hypothetical protein. Homology to Daro03000439 of Dechloromonas aromatica of 48% (gi|41725494|ref|ZP_00152252.1|(NBCI ENTREZ)). No domains predicted. No signal peptide. No TMHs.; hypothetical protein 4335799 4608208 azo3955 Azoarcus sp. BH72 hypothetical protein YP_935457.1 4335032 D 62928 CDS YP_935458.1 119900245 4608209 4335858..4337279 1 NC_008702.1 Conserved hypothetical protein, has weak homlogs in the Database. TrEMBL;Q88I83(25% Identity). gi|46140644|ref|ZP_00152253.2| , 30% Identity to Dechloromonas aromatica RCB,Nucleotidyltransferase/DNA polymerase involved in DNA repair Has PF00817,impB/mucB/samB family; IPR001126, UMUC_like; These proteins are involved in UV protection.In Escherichia coli, UV and many chemicals appear to cause mutagenesis by a process of translesion synthesis that requires DNA polymerase III and the SOS-regulated proteins UmuD, UmuC and RecA. This machinery allows the replication to continue through DNA lesion, and therefore avoid lethal interruption of DNA replication after DNA damage. The UmuC is a well conserved protein in prokaryotes, with a homologue in yeast.; hypothetical protein 4337279 4608209 azo3956 Azoarcus sp. BH72 hypothetical protein YP_935458.1 4335858 D 62928 CDS YP_935459.1 119900246 4608210 4337288..4340482 1 NC_008702.1 Region start changed from 4336170 to 4337262 (-1092 bases); DNA polymerase III subunit alpha 4340482 4608210 azo3957 Azoarcus sp. BH72 DNA polymerase III subunit alpha YP_935459.1 4337288 D 62928 CDS YP_935460.1 119900247 4608211 complement(4340498..4340884) 1 NC_008702.1 Carboxymuconolactone decarboxylase family protein,65% Identity (78% similarity) to TrEMBL;Q8EKK9. Has PF02627, Carboxymuconolactone decarboxylase family; IPR003779, CMD: Carboxymuconolactone decarboxylase (CMD)is involved in protocatechuate catabolism. In some bacteria a gene fusion event leads to expression of CMD with a hydrolase involved in the same pathway. In these bifunctional proteins (e.g. O67982) CMD represents the C-terminal domain, Abhydrolase_1 represents the N-terminal domain.; High confidence in function and specificity; 4-carboxymuconolactone decarboxylase 4340884 pcaC 4608211 pcaC Azoarcus sp. BH72 4-carboxymuconolactone decarboxylase YP_935460.1 4340498 R 62928 CDS YP_935461.1 119900248 4609504 complement(4340829..4341284) 1 NC_008702.1 Putative MerR-family transcriptional regulator,; Family membership; MerR family transcriptional regulator 4341284 cadR 4609504 cadR Azoarcus sp. BH72 MerR family transcriptional regulator YP_935461.1 4340829 R 62928 CDS YP_935462.1 119900249 4608409 complement(4341451..4342317) 1 NC_008702.1 Putative alpha helix protein,yicC.42% identity(57% Similarity) to SwissProt;P23839, TrEMBL;Q8XD97(42%). TrEMBL; Q8XXF8(45% identity). Coils2 program reports the presence of Coiled-Coil. Has PF03755, YicC-like family, N-terminal region; IPR005229, Cons_hypoth255: Family of bacterial proteins. Although poorly characterised, the members of this protein family have been demonstrated to play a role in stationary phase survival. These proteins are not essential during stationary phase.; Conserved hypothetical protein; hypothetical protein 4342317 yicC 4608409 yicC Azoarcus sp. BH72 hypothetical protein YP_935462.1 4341451 R 62928 CDS YP_935463.1 119900250 4610184 4342320..4343360 1 NC_008702.1 Putative serine/threonine protein kinase,; Family membership; putative serine/threonine protein kinase 4343360 4610184 azo3961 Azoarcus sp. BH72 putative serine/threonine protein kinase YP_935463.1 4342320 D 62928 CDS YP_935464.1 119900251 4608212 4343372..4344292 1 NC_008702.1 Putative phosphoprotein phosphatase,; Conserved hypothetical protein; putative phosphoprotein phosphatase 4344292 4608212 azo3962 Azoarcus sp. BH72 putative phosphoprotein phosphatase YP_935464.1 4343372 D 62928 CDS YP_935465.1 119900252 4608213 4344315..4345034 1 NC_008702.1 RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs; ribonuclease PH 4345034 rph 4608213 rph Azoarcus sp. BH72 ribonuclease PH YP_935465.1 4344315 D 62928 CDS YP_935466.1 119900253 4609760 4345038..4345634 1 NC_008702.1 HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine; putative deoxyribonucleotide triphosphate pyrophosphatase 4345634 4609760 azo3964 Azoarcus sp. BH72 putative deoxyribonucleotide triphosphate pyrophosphatase YP_935466.1 4345038 D 62928 CDS YP_935467.1 119900254 4608214 4345631..4346854 1 NC_008702.1 catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III; coproporphyrinogen III oxidase 4346854 4608214 azo3965 Azoarcus sp. BH72 coproporphyrinogen III oxidase YP_935467.1 4345631 D 62928 CDS YP_935468.1 119900255 4608215 complement(4346862..4347371) 1 NC_008702.1 Hypothetical protein predicted by Glimmer/Critica. No homology of the entire protein with the data bank. No domains predicted. No signal peptide. No TMHs; hypothetical protein 4347371 4608215 azo3966 Azoarcus sp. BH72 hypothetical protein YP_935468.1 4346862 R 62928 CDS YP_935469.1 119900256 4608216 complement(4347471..4349312) 1 NC_008702.1 Putative protease IV. homology to sspA of E. coli of 38% (sprot|SPPA_ECOLI). Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane. InterPro: Signal peptide peptidase SppA 67K type (IPR004634); Signal peptide peptidase SppA 36K type (IPR004635); Pepditase family U7 (IPR002142) Pfam: Peptidase family U7 Tigrfam: SppA_67K: signal peptide peptidase SppA; sppA_dom: signal peptide peptidase SppA no signal peptide probable 1 TMH; High confidence in function and specificity; putative protease IV 4349312 4608216 azo3967 Azoarcus sp. BH72 putative protease IV YP_935469.1 4347471 R 62928 CDS YP_935470.1 119900257 4608217 complement(4349397..4350149) 1 NC_008702.1 tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine; tRNA (guanine-N(7)-)-methyltransferase 4350149 trmB 4608217 trmB Azoarcus sp. BH72 tRNA (guanine-N(7)-)-methyltransferase YP_935470.1 4349397 R 62928 CDS YP_935471.1 119900258 4608218 complement(4350146..4350931) 1 NC_008702.1 functions in thiamine (vitamin B1) biosynthesis; in Bacillus subtilis this enzyme catalyzes the formation of thiazole from dehydroxyglycine and 1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate; thiazole synthase 4350931 thiG 4608218 thiG Azoarcus sp. BH72 thiazole synthase YP_935471.1 4350146 R 62928 CDS YP_935472.1 119900259 4608219 complement(4350986..4351189) 1 NC_008702.1 Putative sulfur transfer protein involved in thimanin biosynsthesis.; High confidence in function and specificity; thiamine biosynthesis protein ThiS 4351189 thiS 4608219 thiS Azoarcus sp. BH72 thiamine biosynthesis protein ThiS YP_935472.1 4350986 R 62928 CDS YP_935473.1 119900260 4609939 4351391..4352518 1 NC_008702.1 Catalyzes the conversion of acetyl-CoA and L-homoserine to CoA and O-acetyl-L-homoserine; homoserine O-acetyltransferase 4352518 metX 4609939 metX Azoarcus sp. BH72 homoserine O-acetyltransferase YP_935473.1 4351391 D 62928 CDS YP_935474.1 119900261 4609212 4352521..4353108 1 NC_008702.1 Q8YDE4:20% identity, 37% similarity. Ubiquinone/menaquinone biosynthesis methyltransferase ubiE . Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-14-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-14-benzoquinol (DMQH2) (By similarity). METHIONINE BIOSYNTHESIS PROTEIN Pfam:PPTA(protein prenyl transferase alpha subunit), FERM domain TIGRFAM:rmJ: ribosomal RNA large subunit methylation InterPro IPR000051; SAM_bind. IPR004034; Ubi/men_Metransf. IPR004033; UbiE/COQ5_Metrf. HTH predicted nucleic acid binding motif PROSITE PS01183; UBIE_1; 1. PS01184; UBIE_2; FALSE_NEG.; Family membership; putative methionine biosynthesis protein 4353108 metW 4609212 metW Azoarcus sp. BH72 putative methionine biosynthesis protein YP_935474.1 4352521 D 62928 CDS YP_935475.1 119900262 4609211 complement(4353124..4353927) 1 NC_008702.1 Hypothetical protein. no homology of the entire protein to the data bank. Pfam: putative peptidoglycan binding domain. This domain, peptidoglycan binding domain 1, may have a general peptidoglycan binding function. It is composed of three alpha helices and is found at the N or C terminus of a variety of enzymes involved in bacterial cell wall degradation. no signal peptide. no TMHs; hypothetical protein 4353927 4609211 azo3973 Azoarcus sp. BH72 hypothetical protein YP_935475.1 4353124 R 62928 CDS YP_935476.1 119900263 4608220 complement(4354102..4354419) 1 NC_008702.1 Conserved hypothetical protein. Homology to bb4936 of B. bronchiseptica of 36% (trembl|Q7WDQ2). no domains predicted. no signal peptide. no TMHs; hypothetical protein 4354419 4608220 azo3974 Azoarcus sp. BH72 hypothetical protein YP_935476.1 4354102 R 62928 CDS YP_935477.1 119900264 4608221 4354671..4356191 1 NC_008702.1 AmpG protein. Probably acts as a permease in the beta-lactamase induction system and in peptidoglycan recycling.Belong to the BT1 family of proteins. Presence of DUF domains and signail peptide. 11 tranmembrane helices.36% identity and 50% similarity to E.coli AmpG protein.(JW0423) 2A0125: AmpG-related permease; Family membership; putative AmpG protein 4356191 ampG 4608221 ampG Azoarcus sp. BH72 putative AmpG protein YP_935477.1 4354671 D 62928 CDS YP_935478.1 119900265 4608314 4356228..4356950 1 NC_008702.1 Probable uracil-DNA glycosylase. Has PF03167,Uracil DNA glycosylase superfamily;IPR005122 UDNA_glycsylseSF; Uracil-DNA glycosylase is a DNA repair enzyme that excises uracil residues from DNA by cleaving the N-glycosylic bond. Uracil in DNA can arise as a result of misincorportation of dUMP residues by DNA polymerase or deamination of cytosine. The sequence of uracil-DNA glycosylase is extremely well conserved; uracil-DNA glycosylase 4356950 4608314 azo3976 Azoarcus sp. BH72 uracil-DNA glycosylase YP_935478.1 4356228 D 62928 CDS YP_935479.1 119900266 4608222 4357121..4357792 1 NC_008702.1 Region start changed from 4356747 to 4357095 (-348 bases); putative carbonic anhydrase 4357792 4608222 azo3977 Azoarcus sp. BH72 putative carbonic anhydrase YP_935479.1 4357121 D 62928 CDS YP_935480.1 119900267 4608223 complement(4358238..4360409) 1 NC_008702.1 Primosomal protein N (Replication factor Y). Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB dnaC dnaT and primase. PriA then functions as a helicase within the primosome. InterPro: DEAD/DEAH box helicase; High confidence in function and specificity; primosomal protein N' 4360409 priA 4608223 priA Azoarcus sp. BH72 primosomal protein N' YP_935480.1 4358238 R 62928 CDS YP_935481.1 119900268 4609626 complement(4360801..4361877) 1 NC_008702.1 catalyzes the formation of coproporphyrinogen from uroporphyrinogen III; uroporphyrinogen decarboxylase 4361877 hemE 4609626 hemE Azoarcus sp. BH72 uroporphyrinogen decarboxylase YP_935481.1 4360801 R 62928 CDS YP_935482.1 119900269 4608983 complement(4361965..4363437) 1 NC_008702.1 This proteins are involved in active potassium uptake utilising ATP in the process. TrkH a member of the family TRKH is a hydrophobic membrane protein and determines the specificity and kinetics of cation transport by the TrK system in E. coli.Requires TrkE for transport activity. 38% Cat_transpt.IPR004772; K_transptTrk. Pfam:PF02386; TrkH; 1. TIGRFAMs:TIGR00933; 2a38; 1. TMHelix:10. Signal peptide present.; High confidence in function and specificity; putative potassium system uptake protein 4363437 trkH2 4608983 trkH2 Azoarcus sp. BH72 putative potassium system uptake protein YP_935482.1 4361965 R 62928 CDS YP_935483.1 119900270 4609972 complement(4363437..4364840) 1 NC_008702.1 involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain; potassium transporter peripheral membrane protein 4364840 trkA 4609972 trkA Azoarcus sp. BH72 potassium transporter peripheral membrane protein YP_935483.1 4363437 R 62928 CDS YP_935484.1 119900271 4609970 complement(4364923..4366170) 1 NC_008702.1 Probable nitrogen assimilation regulatory protein,; High confidence in function and specificity; nitrogen assimilation regulatory protein 4366170 ntrX 4609970 ntrX Azoarcus sp. BH72 nitrogen assimilation regulatory protein YP_935484.1 4364923 R 62928 CDS YP_935485.1 119900272 4609415 complement(4366174..4368294) 1 NC_008702.1 Probable nitrogen regulation protein,; High confidence in function and specificity; nitrogen regulation protein 4368294 ntrY 4609415 ntrY Azoarcus sp. BH72 nitrogen regulation protein YP_935485.1 4366174 R 62928 CDS YP_935486.1 119900273 4609416 complement(4368291..4368995) 1 NC_008702.1 Conserved hypothetical secreted protein. Homology to RS02253 of R.solanacearum of 32% (trembl|Q8Y3A4(SRS)). No domains predicted. No TMHs. Signal peptide present.; Conserved hypothetical protein; hypothetical protein 4368995 4609416 azo3984 Azoarcus sp. BH72 hypothetical protein YP_935486.1 4368291 R 62928 CDS YP_935487.1 119900274 4608224 complement(4368856..4370136) 1 NC_008702.1 SUN protein; Conserved hypothetical protein; Sun protein 4370136 4608224 azo3985 Azoarcus sp. BH72 Sun protein YP_935487.1 4368856 R 62928 CDS YP_935488.1 119900275 4608225 complement(4370229..4371083) 1 NC_008702.1 Spermidine synthase counts to a group of polyamine biosynthetic enzymes involved in the fifth (last) step in the biosynthesis of spermidine from arginine and methionine which includes; spermidine synthase, spermine synthase and putrescine N-methyltransferase. Similar to sprot|SPEE_NITEU (39%) and to sprot|SPE1_PSEAE (34%). ProSite (PS50193): SAM (and some other nucleotide) binding motif Pfam (PF01564): Spermidine synthase TIGRFAM: speE,spermidine synthase; Specificity unclear; spermidine synthase 4371083 speE2 4608225 speE2 Azoarcus sp. BH72 spermidine synthase YP_935488.1 4370229 R 62928 CDS YP_935489.1 119900276 4609888 complement(4371199..4371963) 1 NC_008702.1 Conserved hypothetical membrane protein. TREMBL:Q88FZ3: 44% identity, 60% similarity InterPro:IPR002781; DUF81.This domain is found in integral membrane proteins of prokaryotes which are uncharacterized Pfam:PF01925; DUF81 Signal peptide present and presence of 6 transmembrane helices.; Conserved hypothetical protein; hypothetical protein 4371963 4609888 azo3987 Azoarcus sp. BH72 hypothetical protein YP_935489.1 4371199 R 62928 CDS YP_935490.1 119900277 4608226 complement(4372023..4373381) 1 NC_008702.1 in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE; tRNA modification GTPase TrmE 4373381 trmE 4608226 trmE Azoarcus sp. BH72 tRNA modification GTPase TrmE YP_935490.1 4372023 R 62928 CDS YP_935491.1 119900278 4609933 complement(4373353..4374969) 1 NC_008702.1 Inner membrane protein oxaA. Required for the insertion of integral membrane proteins into the membrane. Probably plays an essential role in the integration of proteins of the respiratory chain complexes. Involved in integration of membrane proteins that insert dependently and independently of the Sec translocase complex (by similariety). Pfam: 60 kd inner membran proetin TMHs: 3 Signal peptide: no; High confidence in function and specificity; hypothetical protein 4374969 4609933 azo3989 Azoarcus sp. BH72 hypothetical protein YP_935491.1 4373353 R 62928 CDS YP_935492.1 119900279 4608227 complement(4375023..4375232) 1 NC_008702.1 Conserved hypothetical protein, 62% identical(76% similar) to SwissProt;Q7NPT7. Has PF01809, Domain of unknown function DUF37;IPR002696;This domain is found in short (70 amino acid) hypothetical proteins from various bacteria. The domain contains three conserved cysteine residues. Q44066 from Aeromonas hydrophila has been found to have hemolytic activity (unpublished).; hypothetical protein 4375232 4608227 azo3990 Azoarcus sp. BH72 hypothetical protein YP_935492.1 4375023 R 62928 CDS YP_935493.1 119900280 4608228 complement(4375229..4375591) 1 NC_008702.1 Region start changed from 4375477 to 4375591 (114 bases); ribonuclease P 4375591 4608228 azo3991 Azoarcus sp. BH72 ribonuclease P YP_935493.1 4375229 R 62928 CDS YP_935494.1 119900281 4608229 complement(4375593..4375931) 1 NC_008702.1 Putative Ribosomal protein L34. Has PF00468,Ribosomal protein L34;IPR000271, Ribosomal_L34;Ribosomes are the particles that catalyze mRNA-directed protein synthesis in all organisms. The codons of the mRNA are exposed on the ribosome to allow tRNA binding. This leads to the incorporation of amino acids into the growing polypeptide chain in accordance with the genetic information. Incoming amino acid monomers enter the ribosomal A site in the form of aminoacyl-tRNAs complexed with elongation factor Tu (EF-Tu) and GTP. The growing polypeptide chain, situated in the P site as peptidyl-tRNA, is then transferred to aminoacyl-tRNA and the new peptidyl-tRNA, extended by one residue, is translocated to the P site with the aid the elongation factor G (EF-G) and GTP as the deacylated tRNA is released from the ribosome through one or more exit sites.; Function unclear; putative ribosomal protein L34 4375931 rpmH 4608229 rpmH Azoarcus sp. BH72 putative ribosomal protein L34 YP_935494.1 4375593 R 62928 CDS