-- dump date   	20120504_134435
-- class       	Genbank::Contig
-- table       	contig_comment
-- id	comment
NC_005957.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) wasPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitatsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferousPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of BacillusPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction productPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as aPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans isPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplifiedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl EnvironPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries werePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production GenomicsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat inPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for eachPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walksPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the genePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functionalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basicPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 5540 features have been annotated on thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 5540 features have been annotated on the sequence record.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AE017355. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 24x. Finishing was performed at JGI-LANL starting with 83 contigs and 16 scaffolds. Repetitive regions were identified, assembled and finished by manually checking paired reads close to each repeat in the assembly with consed and then making a subassembly for each repetitive region. Fifty five gaps were closed with primer walks and 16 by PCR.Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 5540 features have been annotated on the sequence record. COMPLETENESS: full length.
NC_006578.1	PROVISIONAL REFSEQ: This record has not yet been subject to finalPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) wasPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34)PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence ofPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitatsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferousPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of BacillusPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction productPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as aPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans isPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensisPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplifiedPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD,PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl EnvironPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries werePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production GenomicsPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. GenePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the genePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions andPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators atPROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 80 genes have been annotated on thePROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 80 genes have been annotated on the sequence record.PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000047. Bacillus thuringiensis 97-27 (subsp. konkukian (serotype H34)) was originally isolated from a case of severe human tissue necrosis (Bacillus thuringiensis subsp. konkukian (serotype H34) superinfection: Case report and experimental evidence of pathogenicity in immunosupporessed mice. Hernandez, E, Ramisse, F, Ducoureau, J-P, Cruel, T, and Cavallo, J-D. J Clin Microbiol 1998 36(7):2138-2139). B. thuringiensis is indigenous to many habitats worldwide; these include soil, insects, deciduous and coniferous leaves (Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Carozzi, NB, Kramer, VC, Warren, GW, Evola, S, and Koziel, MG. Appl Environ Microbiol. 1991 57(11):3057-61). B. thuringiensis is an insect pathogen that is widely used as a biopesticide in commercial agriculture. Infection of humans is unusual. The apparent pathogenic properties of B. thuringiensis 97-27 are very unusual for B. thuringiensis; unlike most B. thuringiensis isolates, this isolate is very closely related to B. anthracis based on phylogenetic analysis (Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates. Hill, KK, Ticknor, LO, Okinaka, RT, Asay, M, Blair, H, Bliss, KA, Laker, M, Pardington, PE, Richardson, AP, Tonks, M, Beecher, DJ, Kemp, JD, Kolsto, A-B, Wong, ACL, Keim, P, and Jackson, PJ. Appl Environ Microbiol 70(2):1068-1080. Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA to a coverage of 15x. Gene predictions were obtained using Glimmer. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, COGS, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. A total of 80 genes have been annotated on the sequence record. COMPLETENESS: full length.