-- dump date 20120504_144658 -- class Genbank::CDS -- table cds -- table main -- field 1 id -- field 2 GI -- field 3 GeneID -- field 4 chrom_position -- field 5 chromosome -- field 6 codon_start -- field 7 contig -- field 8 description -- field 9 end_pos -- field 10 gene -- field 11 gene_id -- field 12 name -- field 13 organism -- field 14 product -- field 15 protein_id -- field 16 start_pos -- field 17 strand -- field 18 taxid -- field 19 type -- header -- id GI GeneID chrom_position chromosome codon_start contig description end_pos gene gene_id name organism product protein_id start_pos strand taxid type YP_666215.1 110669658 4199350 1..1521 1 NC_008245.1 binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.; chromosomal replication initiation protein 1521 dnaA 4199350 dnaA Francisella tularensis subsp. tularensis FSC198 chromosomal replication initiation protein YP_666215.1 1 D 393115 CDS YP_666216.1 110669659 4199357 1558..2661 1 NC_008245.1 Similar to DP3B_ECOLI (P00583) DNA polymerase III,beta chain from E. coli (366 aa). FASTA opt: 826 Z-score: 955.1 E(): 2.6e-45 Smith-Waterman score: 826; 35.230 identity in 369 aa overlap; DNA polymerase III subunit beta 2661 dnaN 4199357 dnaN Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit beta YP_666216.1 1558 D 393115 CDS YP_666217.1 110669660 4199614 complement(join(3204..3662,3662..3988)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 3988 isftu1 4199614 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666217.1 3204 R 393115 CDS YP_666218.1 110669661 4200573 4661..5869 1 NC_008245.1 Similar to Q9ZE69 Proline/betaine transporter (PROP1) from Rickettsia prowazekii (418 aa). FASTA: opt: 605 Z-score: 665.8 E(): 3.4e-29 Smith-Waterman score: 605; 28.293 identity in 410 aa overlap ORF ftt0006; MFS superfamily proline/ betaine transporter 5869 4200573 FTF0006 Francisella tularensis subsp. tularensis FSC198 MFS superfamily proline/ betaine transporter YP_666218.1 4661 D 393115 CDS YP_666219.1 110669662 4199691 6044..7822 1 NC_008245.1 catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes; aspartyl-tRNA synthetase 7822 aspS 4199691 aspS Francisella tularensis subsp. tularensis FSC198 aspartyl-tRNA synthetase YP_666219.1 6044 D 393115 CDS YP_666220.1 110669663 4200578 complement(11450..12232) 1 NC_008245.1 ORF ftt0013c; lipoprotein 12232 4200578 FTF0013c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666220.1 11450 R 393115 CDS YP_666221.1 110669664 4200579 complement(12472..12870) 1 NC_008245.1 ORF ftt0014c; hypothetical protein 12870 4200579 FTF0014c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666221.1 12472 R 393115 CDS YP_666222.1 110669665 4199115 13198..14496 1 NC_008245.1 Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide; adenylosuccinate lyase 14496 purB 4199115 purB Francisella tularensis subsp. tularensis FSC198 adenylosuccinate lyase YP_666222.1 13198 D 393115 CDS YP_666223.1 110669666 4200580 14530..15108 1 NC_008245.1 ORF ftt0016; hypothetical protein 15108 4200580 FTF0016 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666223.1 14530 D 393115 CDS YP_666224.1 110669667 4200581 15195..16736 1 NC_008245.1 Similar to Q8PAK3 Toxin secretion ABC transporter ATP-binding protein from Xanthomonas axonopodis (563 aa). FASTA: opt: 454 Z-score: 516.4 E(): 7.1e-21 Smith-Waterman score: 454; 24.395identity in 496 aa overlap ORF ftt0017; hypothetical protein 16736 4200581 FTF0017 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666224.1 15195 D 393115 CDS YP_666225.1 110669668 4200582 16744..17817 1 NC_008245.1 Similar to Q8PMA5 RND efflux membrane fusion protein from Xanthomonas axonopodis (354 aa). FASTA: opt: 851 Z-score: 971.0 E(): 3.4e-46 Smith-Waterman score: 851; 37.846 identity in 325 aa overlap ORF ftt0018; secretion protein 17817 4200582 FTF0018 Francisella tularensis subsp. tularensis FSC198 secretion protein YP_666225.1 16744 D 393115 CDS YP_666226.1 110669669 4199275 17900..18181 1 NC_008245.1 Similar to Q82T57 Glu-tRNAGln amidotransferase C subunit from Nitrosomonas europaea (95 aa). FASTA:i opt: 188 Z-score: 259.9 E(): 1.4e-06 Smith-Waterman score: 188; 31.818 identity in 88 aa overlap; Glu-tRNAGln amidotransferase C subunit 18181 gatC 4199275 gatC Francisella tularensis subsp. tularensis FSC198 Glu-tRNAGln amidotransferase C subunit YP_666226.1 17900 D 393115 CDS YP_666227.1 110669670 4199273 18190..19635 1 NC_008245.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit A 19635 gatA 4199273 gatA Francisella tularensis subsp. tularensis FSC198 aspartyl/glutamyl-tRNA amidotransferase subunit A YP_666227.1 18190 D 393115 CDS YP_666228.1 110669671 4199274 19638..21059 1 NC_008245.1 allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; aspartyl/glutamyl-tRNA amidotransferase subunit B 21059 gatB 4199274 gatB Francisella tularensis subsp. tularensis FSC198 aspartyl/glutamyl-tRNA amidotransferase subunit B YP_666228.1 19638 D 393115 CDS YP_666229.1 110669672 4200583 21074..21481 1 NC_008245.1 ORF ftt0022; hypothetical protein 21481 4200583 FTF0022 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666229.1 21074 D 393115 CDS YP_666230.1 110669673 4200584 complement(21497..22378) 1 NC_008245.1 Similar to Q9F7Y6 Glycerophospholipid-cholesterol acyltransferase from Aeromonas salmonicida (336 aa). FASTA: opt: 367 Z-score: 437.4 E(): 1.8e-16 Smith-Waterman score: 407; 30.183 identity in 328 aa overlap ORF ftt0023c; lipase/acyltransferase 22378 4200584 FTF0023c Francisella tularensis subsp. tularensis FSC198 lipase/acyltransferase YP_666230.1 21497 R 393115 CDS YP_666231.1 110669674 4200585 complement(22646..22990) 1 NC_008245.1 ORF ftt0024c; hypothetical protein 22990 4200585 FTF0024c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666231.1 22646 R 393115 CDS YP_666232.1 110669675 4200586 complement(23038..24567) 1 NC_008245.1 ORF ftt0025c; hypothetical protein 24567 4200586 FTF0025c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666232.1 23038 R 393115 CDS YP_666233.1 110669676 4200587 complement(24652..25842) 1 NC_008245.1 Similar to Q9HWP5 Probable MFS transporter from Pseudomonas aeruginosa (402 aa). FASTA: opt: 568 Z-score: 586.0 E(): 9.5e-25 Smith-Waterman score: 568; 26.425 identity in 386 aa overlap ORF ftt0026c; hypothetical protein 25842 4200587 FTF0026c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666233.1 24652 R 393115 CDS YP_666234.1 110669677 4199652 complement(25858..27111) 1 NC_008245.1 Similar to DCDA_BACSU (P23630) Diaminopimelate decarboxylase from Bacillus subtilis (441 aa). FASTA: opt: 437 Z-score: 538.0 E(): 4.5e-22 Smith-Waterman score: 437; 27.295 identity in 414 aa overlap; diaminopimelate decarboxylase 27111 lysA1 4199652 lysA1 Francisella tularensis subsp. tularensis FSC198 diaminopimelate decarboxylase YP_666234.1 25858 R 393115 CDS YP_666235.1 110669678 4200588 complement(27104..28366) 1 NC_008245.1 Similar to YCEE_ECOLI (P25744) Hypothetical transport protein yce from E. coli (408 aa). FASTA: opt: 364 Z-score: 408.7 E(): 7.1e-15 Smith-Waterman score: 450; 26.582 identity in 395 aa overlap ORF ftt0028c; hypothetical protein 28366 4200588 FTF0028c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666235.1 27104 R 393115 CDS YP_666236.1 110669679 4200589 complement(28373..30292) 1 NC_008245.1 Similar to AAO89973 (Q83EA5) FrgA protein from Coxiella burnetii (528 aa). FASTA: opt: 1047 Z-score: 1237.4 E(): 4.9e-61 Smith-Waterman score: 1053; 34.051 identity in 511 aa overlap ORF ftt0029c; hypothetical protein 30292 4200589 FTF0029c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666236.1 28373 R 393115 CDS YP_666237.1 110669680 4199202 complement(30545..30967) 1 NC_008245.1 Similar to FUR_ECOLI (P06975) Ferric uptake regulation protein from E. coli (148 aa). FASTA: opt: 396 Z-score: 515.7 E(): 7.8e-21 Smith-Waterman score: 396; 40.714 identity in 140 aa overlap; ferric uptake regulation protein 30967 fur 4199202 fur Francisella tularensis subsp. tularensis FSC198 ferric uptake regulation protein YP_666237.1 30545 R 393115 CDS YP_666238.1 110669681 4200245 31176..31574 1 NC_008245.1 Similar to Q9A6X0 NADH dehydrogenase I, A subunit from Caulobacter crescentus (125 aa). FASTA: opt: 398 Z-score: 508.1 E(): 2.1e-20 Smith-Waterman score: 398; 46.565 identity in 131 aa overlap; NADH dehydrogenase I subunit A 31574 nuoA 4200245 nuoA Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit A YP_666238.1 31176 D 393115 CDS YP_666239.1 110669682 4200246 31565..32041 1 NC_008245.1 The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen; NADH dehydrogenase subunit B 32041 nuoB 4200246 nuoB Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase subunit B YP_666239.1 31565 D 393115 CDS YP_666240.1 110669683 4200247 32038..32688 1 NC_008245.1 Similar to NUOC_NEIMB (Q9K1C1) NADH-quinone oxidoreductase chain from Neisseria meningitidis (197 aa). FASTA: opt: 516 Z-score: 659.3 E(): 7.8e-29 Smith-Waterman score: 568; 44.286identity in 210 aa overlap; NADH dehydrogenase I 32688 nuoC 4200247 nuoC Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I YP_666240.1 32038 D 393115 CDS YP_666241.1 110669684 4200248 32710..33963 1 NC_008245.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit D 33963 nuoD 4200248 nuoD Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase subunit D YP_666241.1 32710 D 393115 CDS YP_666242.1 110669685 4200249 33973..34461 1 NC_008245.1 Similar to Q83BQ9 NADH dehydrogenase I, E subunit from Xanthomonas campestris (174 aa). FASTA: opt: 528 Z-score: 689.7 E(): 1.6e-30 Smith-Waterman score: 528; 52.318 identity in 151 aa overlap; NADH dehydrogenase I subunit E 34461 nuoE 4200249 nuoE Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit E YP_666242.1 33973 D 393115 CDS YP_666243.1 110669686 4200745 34468..35742 1 NC_008245.1 Similar to Q83BR0 NADH dehydrogenase I, F subunit from Coxiella burnetti (422 aa). FASTA: opt: 1851 Z-score: 2306.0 E(): 1.5e-120 Smith-Waterman score: 1851; 61.848 identity in 422 aa overlap; NADH dehydrogenase I subunit F 35742 nuoF 4200745 nuoF Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit F YP_666243.1 34468 D 393115 CDS YP_666244.1 110669687 4200746 35760..38126 1 NC_008245.1 Catalyzes the transfer of electrons from NADH to ubiquinone; NADH dehydrogenase subunit G 38126 nuoG 4200746 nuoG Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase subunit G YP_666244.1 35760 D 393115 CDS YP_666245.1 110669688 4200747 38128..39138 1 NC_008245.1 Similar to Q83BR2 NADH dehydrogenase I, H subunit from Coxiella burnetii (340 aa). FASTA: opt: 1510 Z-score: 1695.3 E(): 1.5e-86 Smith-Waterman score: 1510; 60.843 identity in 332 aa overlap; NADH dehydrogenase I subunit H 39138 nuoH 4200747 nuoH Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit H YP_666245.1 38128 D 393115 CDS YP_666246.1 110669689 4200748 39152..39640 1 NC_008245.1 Catalyzes the transfer of electrons from NADH to quinone; NADH dehydrogenase subunit I 39640 nuoI 4200748 nuoI Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase subunit I YP_666246.1 39152 D 393115 CDS YP_666247.1 110669690 4200749 39645..40250 1 NC_008245.1 Similar to Q9K1B2 NADH dehydrogenase I, J subunit from Neisseria meningitidis (223 aa). FASTA: opt: 387 Z-score: 452.6 E(): 2.6e-17 Smith-Waterman score: 387; 38.191 identity in 199 aa overlap; NADH dehydrogenase I subunit J 40250 nuoJ 4200749 nuoJ Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit J YP_666247.1 39645 D 393115 CDS YP_666248.1 110669691 4200750 40228..40560 1 NC_008245.1 Similar to Q9A6Y6 NADH dehydrogenase I, K subunit from Caulobacter crescentus (101 aa). FASTA: opt: 300 Z-score: 405.7 E(): 1e-14 Smith-Waterman score: 300; 43.000 identity in 100 aa overlap; NADH dehydrogenase I subunit K 40560 nuoK 4200750 nuoK Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit K YP_666248.1 40228 D 393115 CDS YP_666249.1 110669692 4200751 40570..42579 1 NC_008245.1 Similar to Q83BR6 NADH dehydrogenase I, L subunit from Coxiella burnetii (653 aa). FASTA: opt: 1934 Z-score: 2065.6 E(): 3.6e-107 Smith-Waterman score: 2090; 50.760 identity in 658 aa overlap; NADH dehydrogenase I subunit L 42579 nuoL 4200751 nuoL Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit L YP_666249.1 40570 D 393115 CDS YP_666250.1 110669693 4200752 42605..44194 1 NC_008245.1 Similar to Q83BR7 NADH dehydrogenase I, M subunit from Coxiella burnetii (506 aa). FASTA: opt: 1099 Z-score: 1147.9 E(): 4.8e-56 Smith-Waterman score: 1659; 47.228 identity in 523 aa overlap; NADH dehydrogenase I subunit M 44194 nuoM 4200752 nuoM Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit M YP_666250.1 42605 D 393115 CDS YP_666251.1 110669694 4200753 44204..45661 1 NC_008245.1 Similar to Q83BR8 NADH dehydrogenase I, N subunit from Coxiella burnetii (482 aa). FASTA: opt: 1231 Z-score: 1222.9 E(): 3.2e-60 Smith-Waterman score: 1231; 39.914 identity in 466 aa overlap; NADH dehydrogenase I subunit N 45661 nuoN 4200753 nuoN Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase I subunit N YP_666251.1 44204 D 393115 CDS YP_666252.1 110669695 4200590 45737..45931 1 NC_008245.1 Similar to Q8DDC7 Conserved hypothetical protein from Vibrio vulnificus (83 aa). FASTA: opt: 165 Z-score: 224.7 E(): 0.00013 Smith-Waterman score: 165; 45.763 identity in 59 aa overlap ORF ftt0045; hypothetical protein 45931 4200590 FTF0045 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666252.1 45737 D 393115 CDS YP_666253.1 110669696 4200374 47478..48512 1 NC_008245.1 catalyzes the formation of coproporphyrinogen from uroporphyrinogen III; uroporphyrinogen decarboxylase 48512 hemE 4200374 hemE Francisella tularensis subsp. tularensis FSC198 uroporphyrinogen decarboxylase YP_666253.1 47478 D 393115 CDS YP_666254.1 110669697 4199153 48732..49190 1 NC_008245.1 Similar to Q88DV5 Conserved hypothetical protein from Pseudomonas putida (169 aa). FASTA: opt: 402 Z-score: 490.7 E(): 1.7e-19 Smith-Waterman score: 402; 43.243 identity in 148 aa overlap ORF ftt0048; hypothetical protein 49190 4199153 FTF0048 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666254.1 48732 D 393115 CDS YP_666255.1 110669698 4200757 49208..50677 1 NC_008245.1 modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination; transcription elongation factor NusA 50677 nusA 4200757 nusA Francisella tularensis subsp. tularensis FSC198 transcription elongation factor NusA YP_666255.1 49208 D 393115 CDS YP_666256.1 110669699 4199610 50716..53256 1 NC_008245.1 Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex; translation initiation factor IF-2 53256 infB 4199610 infB Francisella tularensis subsp. tularensis FSC198 translation initiation factor IF-2 YP_666256.1 50716 D 393115 CDS YP_666257.1 110669700 4200596 53259..53690 1 NC_008245.1 Similar to RBFA_ECOLI (P09170) Ribosome-binding factor A from E. coli (132 aa). FASTA: opt: 301 Z-score: 381.1 E(): 2.5e-13 Smith-Waterman score: 301; 41.935 identity in 124 aa overlap; ribosome-binding factor A 53690 rbfA 4200596 rbfA Francisella tularensis subsp. tularensis FSC198 ribosome-binding factor A YP_666257.1 53259 D 393115 CDS YP_666258.1 110669701 4200195 53698..54963 1 NC_008245.1 catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG; histidyl-tRNA synthetase 54963 hisS 4200195 hisS Francisella tularensis subsp. tularensis FSC198 histidyl-tRNA synthetase YP_666258.1 53698 D 393115 CDS YP_666259.1 110669702 4199154 54970..56268 1 NC_008245.1 Similar to AAO90449 (Q83D24) Major facilitator family transporter from Coxiella burnetii (437 aa). FASTA: opt: 908 Z-score: 1023.7 E(): 3.6e-49 Smith-Waterman score: 909; 36.603 identity in 418 aa overlap ORF ftt0053; major facilitator transporter 56268 4199154 FTF0053 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666259.1 54970 D 393115 CDS YP_666260.1 110669703 4199155 56271..57338 1 NC_008245.1 Similar to YHCM_ECOLI (P46442) Hypothetical protein yhcM from E. coli (375 aa). FASTA: opt: 872 Z-score: 1033.2 E(): 1.2e-49 Smith-Waterman score: 898; 39.833 identity in 359 aa overlap ORF ftt0054; ATPase 57338 4199155 FTF0054 Francisella tularensis subsp. tularensis FSC198 ATPase YP_666260.1 56271 D 393115 CDS YP_666261.1 110669704 4199222 57331..58260 1 NC_008245.1 Similar to RLUC_ECOLI (P23851) Ribosomal large subunit pseudouridine synthase C from E. coli (319 aa). FASTA: opt: 791 Z-score: 972.5 E(): 2.8e-46 Smith-Waterman score: 791; 40.777 identity in 309 aa overlap; ribosomal large subunit pseudouridine synthase C 58260 rluC 4199222 rluC Francisella tularensis subsp. tularensis FSC198 ribosomal large subunit pseudouridine synthase C YP_666261.1 57331 D 393115 CDS YP_666262.1 110669705 4199156 complement(58262..59566) 1 NC_008245.1 Similar to Q83D24 (Q83D24) Major facilitator family transporter from Coxiella burnetii (437 aa). FASTA: opt: 1028 Z-score: 1172.6 E(): 2e-57 Smith-Waterman score: 1036; 40.187 identity in 428 aa overlap ORF ftt0056c; major facilitator transporter 59566 4199156 FTF0056c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666262.1 58262 R 393115 CDS YP_666263.1 110669706 4199157 59643..60074 1 NC_008245.1 ORF ftt0057; hypothetical protein 60074 4199157 FTF0057 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666263.1 59643 D 393115 CDS YP_666264.1 110669707 4199693 60127..60918 1 NC_008245.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0; F0F1 ATP synthase subunit A 60918 atpB 4199693 atpB Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit A YP_666264.1 60127 D 393115 CDS YP_666265.1 110669708 4199696 60969..61274 1 NC_008245.1 produces ATP from ADP in the presence of a proton gradient across the membrane; subunit C is part of the membrane proton channel F0; F0F1 ATP synthase subunit C 61274 atpE 4199696 atpE Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit C YP_666265.1 60969 D 393115 CDS YP_666266.1 110669709 4199697 61320..61790 1 NC_008245.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.; F0F1 ATP synthase subunit B 61790 atpF 4199697 atpF Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit B YP_666266.1 61320 D 393115 CDS YP_666267.1 110669710 4199699 61810..62334 1 NC_008245.1 Produces ATP from ADP in the presence of a proton gradient across the membrane; the delta subunit is part of the catalytic core of the ATP synthase complex; F0F1 ATP synthase subunit delta 62334 atpH 4199699 atpH Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit delta YP_666267.1 61810 D 393115 CDS YP_666268.1 110669711 4199692 62353..63894 1 NC_008245.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit; F0F1 ATP synthase subunit alpha 63894 atpA 4199692 atpA Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit alpha YP_666268.1 62353 D 393115 CDS YP_666269.1 110669712 4199698 63909..64805 1 NC_008245.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the gamma chain is a regulatory subunit; F0F1 ATP synthase subunit gamma 64805 atpG 4199698 atpG Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit gamma YP_666269.1 63909 D 393115 CDS YP_666270.1 110669713 4199695 64815..66191 1 NC_008245.1 Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit; F0F1 ATP synthase subunit beta 66191 atpD 4199695 atpD Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit beta YP_666270.1 64815 D 393115 CDS YP_666271.1 110669714 4199694 66204..66641 1 NC_008245.1 produces ATP from ADP in the presence of a proton gradient across the membrane; the epsilon subunit is part of the catalytic core of the ATP synthase complex; F0F1 ATP synthase subunit epsilon 66641 atpC 4199694 atpC Francisella tularensis subsp. tularensis FSC198 F0F1 ATP synthase subunit epsilon YP_666271.1 66204 D 393115 CDS YP_666272.1 110669715 4199158 66852..69695 1 NC_008245.1 ORF ftt0066; hypothetical protein 69695 4199158 FTF0066 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666272.1 66852 D 393115 CDS YP_666273.1 110669716 4199159 complement(69712..70041) 1 NC_008245.1 Similar to Q8D879 Glutaredoxin-related protein from Vibrio vulnificus (112 aa). FASTA: opt: 485 Z-score: 630.6 E(): 2.8e-27 Smith-Waterman score: 485; 65.306 identity in 98 aa overlap ORF ftt0067c; glutaredoxin-like protein 70041 4199159 FTF0067c Francisella tularensis subsp. tularensis FSC198 glutaredoxin-like protein YP_666273.1 69712 R 393115 CDS YP_666274.1 110669717 4199077 70177..70755 1 NC_008245.1 Fe; Similar to SODF_ECOLI (P09157) Superoxide dismutase [Fe] from E. coli (192 aa). FASTA: opt: 896 Z-score: 1143.2 E(): 8.8e-56 Smith-Waterman score: 896; 66.316 identity in 190 aa overlap; superoxide dismutase 70755 sodB 4199077 sodB Francisella tularensis subsp. tularensis FSC198 superoxide dismutase YP_666274.1 70177 D 393115 CDS YP_666275.1 110669718 4199160 complement(70763..70948) 1 NC_008245.1 ORF ftt0069c; hypothetical protein 70948 4199160 FTF0069c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666275.1 70763 R 393115 CDS YP_666276.1 110669719 4199422 complement(70960..72225) 1 NC_008245.1 Similar to AMPG_ECOLI (P36670) AmpG protein from E. coli (491 aa). FASTA: opt: 996 Z-score: 1121.1 E(): 1.5e-54 Smith-Waterman score: 996; 38.614 identity in 404 aa overlap; major facilitator superfamily transporter 72225 ampG 4199422 ampG Francisella tularensis subsp. tularensis FSC198 major facilitator superfamily transporter YP_666276.1 70960 R 393115 CDS YP_666277.1 110669720 4199799 complement(72314..73588) 1 NC_008245.1 Similar to CISY_PSEAE (P14165) Citrate synthase from Pseudomonas aeruginosa (428 aa). FASTA: opt: 1540 Z-score: 1956.3 E(): 4.5e-101 Smith-Waterman score: 1540; 57.353 identity in 408 aa overlap; citrate synthase 73588 gltA 4199799 gltA Francisella tularensis subsp. tularensis FSC198 citrate synthase YP_666277.1 72314 R 393115 CDS YP_666278.1 110669721 4200731 73831..74304 1 NC_008245.1 Similar to Q884A1 Succinate dehydrogenase,cytochrome b556 subunit from Pseudomonas syringae (124 aa). FASTA: opt: 332 Z-score: 413.6 E(): 3.8e-15 Smith-Waterman score: 332; 39.844identity in 128 aa overlap; succinate dehydrogenase, cytochrome b556 74304 sdhC 4200731 sdhC Francisella tularensis subsp. tularensis FSC198 succinate dehydrogenase, cytochrome b556 YP_666278.1 73831 D 393115 CDS YP_666279.1 110669722 4200732 74280..74648 1 NC_008245.1 Similar to Q88FA6 Succinate dehydrogenase,hydrophobic membrane anchor protein from Pseudomonas putida (122 aa). FASTA: opt: 242 Z-score: 306.8 E(): 3.4e-09 Smith-Waterman score: 242; 34.677 identity in 124 aa overlap; succinate dehydrogenase hydrophobic membrane anchor protein 74648 sdhD 4200732 sdhD Francisella tularensis subsp. tularensis FSC198 succinate dehydrogenase hydrophobic membrane anchor protein YP_666279.1 74280 D 393115 CDS YP_666280.1 110669723 4200729 74661..76454 1 NC_008245.1 Similar to Q9I3D5 Succinate dehydrogenase (A subunit) from Pseudomonas aeruginosa (590 aa). FASTA: opt: 2468 Z-score: 2874.7 E(): 3.2e-152 Smith-Waterman score: 2468; 61.139 identity in 597 aa overlap; succinate dehydrogenase, catalytic and NAD/flavoprotein subunit 76454 sdhA 4200729 sdhA Francisella tularensis subsp. tularensis FSC198 succinate dehydrogenase, catalytic and NAD/flavoprotein subunit YP_666280.1 74661 D 393115 CDS YP_666281.1 110669724 4200730 76472..77173 1 NC_008245.1 part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase; succinate dehydrogenase iron-sulfur subunit 77173 sdhB 4200730 sdhB Francisella tularensis subsp. tularensis FSC198 succinate dehydrogenase iron-sulfur subunit YP_666281.1 76472 D 393115 CDS YP_666282.1 110669725 4199087 77191..80016 1 NC_008245.1 SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide; 2-oxoglutarate dehydrogenase E1 component 80016 sucA 4199087 sucA Francisella tularensis subsp. tularensis FSC198 2-oxoglutarate dehydrogenase E1 component YP_666282.1 77191 D 393115 CDS YP_666283.1 110669726 4199088 80042..81511 1 NC_008245.1 Similar to Q8EFN9 2-oxoglutarate dehydrogenase,E2 component, dihydrolipoamide succinyltransferase from Shewanella oneidensis (395 aa). FASTA: opt: 1379 Z-score: 1563.0 E(): 3.6e-79 Smith-Waterman score: 1379; 55.949 identity in 395 aa overlap; dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex 81511 sucB 4199088 sucB Francisella tularensis subsp. tularensis FSC198 dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex YP_666283.1 80042 D 393115 CDS YP_666284.1 110669727 4199429 81551..82078 1 NC_008245.1 catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis; adenine phosphoribosyltransferase 82078 apt 4199429 apt Francisella tularensis subsp. tularensis FSC198 adenine phosphoribosyltransferase YP_666284.1 81551 D 393115 CDS YP_666285.1 110669728 4199855 82089..83420 1 NC_008245.1 catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate; phosphoglucosamine mutase 83420 glmM 4199855 glmM Francisella tularensis subsp. tularensis FSC198 phosphoglucosamine mutase YP_666285.1 82089 D 393115 CDS YP_666286.1 110669729 4199859 83423..84184 1 NC_008245.1 Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate; triosephosphate isomerase 84184 tpiA 4199859 tpiA Francisella tularensis subsp. tularensis FSC198 triosephosphate isomerase YP_666286.1 83423 D 393115 CDS YP_666287.1 110669730 4200739 84172..84525 1 NC_008245.1 Similar to Q9KU83 Preprotein translocase, SecG subunit from Vibrio cholerae (111 aa). FASTA: opt: 283 Z-score: 369.6 E(): 1.1e-12 Smith-Waterman score: 289; 45.299identity in 117 aa overlap. Together with SecE and SecY forming an integral membrane complex.; preprotein translocase subunit SecG 84525 secG 4200739 secG Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecG YP_666287.1 84172 D 393115 CDS YP_666288.1 110669731 4199162 85736..86212 1 NC_008245.1 ORF ftt0083; hypothetical protein 86212 4199162 FTF0083 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666288.1 85736 D 393115 CDS YP_666289.1 110669732 4200379 complement(86209..87351) 1 NC_008245.1 Similar to Q87V87 Oxygen-independent coproporphyrinogen III oxidase,putative,from Pseudomonas syringae (404 aa). FASTA: opt: 1156 Z-score: 1386.6 E(): 2.4e-69 Smith-Waterman score: 1156; 46.791 identity in 374 aa overlap; oxygen-independent coproporphyrinogen III oxidase 87351 hemN 4200379 hemN Francisella tularensis subsp. tularensis FSC198 oxygen-independent coproporphyrinogen III oxidase YP_666289.1 86209 R 393115 CDS YP_666290.1 110669733 4199163 complement(87356..87733) 1 NC_008245.1 Similar to YWCD_BACSU P39602 Hypothetical protein ywcD from Bacillus subtilis (127 aa). FASTA: opt: 162 Z-score: 223.9 E(): 0.00013 Smith-Waterman score: 162; 26.667 identity in 120 aa overlap ORF ftt0085c; hypothetical protein 87733 4199163 FTF0085c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666290.1 87356 R 393115 CDS YP_666291.1 110669734 4199164 87803..88681 1 NC_008245.1 Similar to Q9I078 Hypothetical protein PA2765 from Pseudomonas aeruginosa (299 aa). FASTA: opt: 656 Z-score: 793.3 E(): 2.4e-36 Smith-Waterman score: 656; 36.678 identity in 289 aa overlap ORF ftt0086; hypothetical protein 88681 4199164 FTF0086 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666291.1 87803 D 393115 CDS YP_666292.1 110669735 4199752 88786..91599 1 NC_008245.1 Catalyzes the conversion of citrate to isocitrate; aconitate hydratase 91599 acnA 4199752 acnA Francisella tularensis subsp. tularensis FSC198 aconitate hydratase YP_666292.1 88786 D 393115 CDS YP_666293.1 110669736 4199733 91694..92722 1 NC_008245.1 Similar to Q8EBZ4 Twitching motility protein PilT from Shewanella oneidensis (345 aa). FASTA: opt: 1226 Z-score: 1391.3 E(): 1.3e-69 Smith-Waterman score: 1226; 57.186 identity in 334 aa overlap Required for pili retraction probably by filament disassembly. Homologous to pilB but which has the opposite effect. Unique to type IV pili.; Type IV pili nucleotide-binding protein 92722 pilT 4199733 pilT Francisella tularensis subsp. tularensis FSC198 Type IV pili nucleotide-binding protein YP_666293.1 91694 D 393115 CDS YP_666294.1 110669737 4199166 complement(93784..94230) 1 NC_008245.1 Similar to Q87GR3 Hypothetical protein from Vibrio parahaemolyticus (148 aa). FASTA: opt: 160 Z-score: 212.3 E(): 0.00057 Smith-Waterman score: 160; 29.104 identity in 134 aa overlap ORF ftt0090c; hypothetical protein 94230 4199166 FTF0090c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666294.1 93784 R 393115 CDS YP_666295.1 110669738 4199427 complement(94241..95203) 1 NC_008245.1 Similar to Q87H27 Cytochrome BD2, subunit II from Vibrio parahaemolyticus (335 aa). FASTA: opt: 573 Z-score: 669.5 E(): 1.9e-29 Smith-Waterman score: 750; 34.743 identity in 331 aa overlap; cytochrome oxidase bd-II subunit II 95203 appB 4199427 appB Francisella tularensis subsp. tularensis FSC198 cytochrome oxidase bd-II subunit II YP_666295.1 94241 R 393115 CDS YP_666296.1 110669739 4199167 96819..97700 1 NC_008245.1 Similar to Q8FFI5 Hypothetical protein yfcH from E. coli (297 aa). FASTA: opt: 498 Z-score: 611.0 E(): 3.8e-26 Smith-Waterman score: 498; 32.673 identity in 303 aa overlap ORF ftt0093; hypothetical protein 97700 4199167 FTF0093 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666296.1 96819 D 393115 CDS YP_666297.1 110669740 4200593 complement(97697..99124) 1 NC_008245.1 Similar to AAO90737 (Q83CA0) Sensor histidine kinase from Coxiella burnetii (478 aa). FASTA: opt: 653 Z-score: 752.8 E(): 4.9e-34 Smith-Waterman score: 677; 29.083 identity in 447 aa overlap Sensory histidine kinase in two-component regulatory system with QseB, regulates flagella and motility by quorum sensing in E. coli,according to Q8X524; sensor histidine kinase 99124 qseC 4200593 qseC Francisella tularensis subsp. tularensis FSC198 sensor histidine kinase YP_666297.1 97697 R 393115 CDS YP_666298.1 110669741 4199168 99240..100196 1 NC_008245.1 ORF ftt0095; hypothetical protein 100196 4199168 FTF0095 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666298.1 99240 D 393115 CDS YP_666299.1 110669742 4199169 100438..101478 1 NC_008245.1 ORF ftt0096; hypothetical protein 101478 4199169 FTF0096 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666299.1 100438 D 393115 CDS YP_666300.1 110669743 4199170 101612..102157 1 NC_008245.1 ORF ftt0097; hypothetical protein 102157 4199170 FTF0097 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666300.1 101612 D 393115 CDS YP_666301.1 110669744 4199616 complement(join(102231..102689,102689..103015)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 103015 isftu1 4199616 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666301.1 102231 R 393115 CDS YP_666302.1 110669745 4200189 103117..103860 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 103860 isftu2 4200189 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666302.1 103117 D 393115 CDS YP_666303.1 110669746 4200535 104530..105543 1 NC_008245.1 Similar to Y402_RICPR (Q9ZDC9) Hypothetical protein RP402 from Rickettsia prowazekii (317 aa). FASTA: opt: 431 Z-score: 518.0 E(): 5.9e-21 Smith-Waterman score: 432; 28.909 identity in 339 aa overlap ORF ftt0101; hypothetical protein 105543 4200535 FTF0101 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666303.1 104530 D 393115 CDS YP_666304.1 110669747 4200537 complement(106988..107569) 1 NC_008245.1 ORF ftt0103c; hypothetical protein 107569 4200537 FTF0103c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666304.1 106988 R 393115 CDS YP_666305.1 110669748 4200538 complement(107814..109091) 1 NC_008245.1 Similar to Q83DX6 Major facilitator family transporter from Coxiella burnetii (421 aa). FASTA: opt: 995 Z-score: 1049.4 E(): 1.5e-50 Smith-Waterman score: 995; 39.066identity in 407 aa overlap ORF ftt0104c; major facilitator transporter 109091 4200538 FTF0104c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666305.1 107814 R 393115 CDS YP_666306.1 110669749 4200539 complement(109223..112336) 1 NC_008245.1 Similar to AAO90606 (Q83CM1) Transporter,AcrB/AcrD/AcrF family from Coxiella burnetii (1019 aa). FASTA: opt: 2662 Z-score: 2840.6 E(): 2.3e-150 Smith-Waterman score: 3071; 45.171identity in 1025 aa overlap ORF ftt0105c; AcrB/AcrD/AcrF family transporter 112336 4200539 FTF0105c Francisella tularensis subsp. tularensis FSC198 AcrB/AcrD/AcrF family transporter YP_666306.1 109223 R 393115 CDS YP_666307.1 110669750 4200540 complement(112336..113709) 1 NC_008245.1 Similar to Q83CM0 Efflux transporter, RND family,MFP subunit from Coxiella burnetti (380 aa). FASTA: opt: 732 Z-score: 824.0 E(): 5.2e-38 Smith-Waterman score: 781; 34.748identity in 377 aa overlap ORF ftt0106c; RND efflux transporter 113709 4200540 FTF0106c Francisella tularensis subsp. tularensis FSC198 RND efflux transporter YP_666307.1 112336 R 393115 CDS YP_666308.1 110669751 4199360 complement(113722..114213) 1 NC_008245.1 Similar to DSBB_NEIMB (Q9JYC6) Disulfide bond formation protein B (disuphide oxidoreductase) from Neisseria meningitidis (162 aa). FASTA: opt: 171 Z-score: 223.1 E(): 0.00014 Smith-Waterman score: 171; 27.344 identity in 128 aa overlap; disulfide bond formation protein 114213 dsbB 4199360 dsbB Francisella tularensis subsp. tularensis FSC198 disulfide bond formation protein YP_666308.1 113722 R 393115 CDS YP_666309.1 110669752 4200318 complement(114253..115335) 1 NC_008245.1 catalyzes the addition and repair of the 3'-terminal CCA sequence in tRNA; these proteins belong to the CCA-adding enzyme subfamily 2 which does not have phosphohydrolase activity; tRNA CCA-pyrophosphorylase 115335 cca 4200318 cca Francisella tularensis subsp. tularensis FSC198 tRNA CCA-pyrophosphorylase YP_666309.1 114253 R 393115 CDS YP_666310.1 110669753 4199856 115440..117269 1 NC_008245.1 lipid A transport protein, ABC transporter,ATP-binding and membrane protein. Similar to Q47908 ValA from Francisella novicida strain U112 (572 aa). FASTA: opt: 3334 Z-score: 3684.8 E(): 2.4e-197 Smith-Waterman score: 3334; 97.810 identity in 548 aa overlap (54-601:13-559). Similar to MSBA_HAEIN (P44407) Lipid A export ATP-binding protein from Haemophilus influenzae (587 aa). FASTA: opt: 1570 Z-score: 1735.1 E(): 9.4e-89 Smith-Waterman score: 1570; 41.913identity in 575 aa overlap.; lipid A transport protein ABC transporter ATP-binding protein/permease 117269 msbA 4199856 msbA Francisella tularensis subsp. tularensis FSC198 lipid A transport protein ABC transporter ATP-binding protein/permease YP_666310.1 115440 D 393115 CDS YP_666311.1 110669754 4199650 117275..118243 1 NC_008245.1 transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate; tetraacyldisaccharide 4'-kinase 118243 lpxK 4199650 lpxK Francisella tularensis subsp. tularensis FSC198 tetraacyldisaccharide 4'-kinase YP_666311.1 117275 D 393115 CDS YP_666312.1 110669755 4199736 118339..121032 1 NC_008245.1 Similar to AAO91288 (Q83AT5) DNA polymerase I from Coxiella burnetii (895 aa). FASTA: opt: 2961 Z-score: 3329.8 E(): 1.4e-177 Smith-Waterman score: 2961; 52.058 identity in 899 aa overlap; DNA polymerase I 121032 polA 4199736 polA Francisella tularensis subsp. tularensis FSC198 DNA polymerase I YP_666312.1 118339 D 393115 CDS YP_666313.1 110669756 4200541 121052..121828 1 NC_008245.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase 121828 4200541 FTF0112 Francisella tularensis subsp. tularensis FSC198 pantothenate kinase YP_666313.1 121052 D 393115 CDS YP_666314.1 110669757 4199344 121833..123077 1 NC_008245.1 catalyzes the transfer of phosphate between the C1 and C5 carbons of pentose; phosphopentomutase 123077 deoB 4199344 deoB Francisella tularensis subsp. tularensis FSC198 phosphopentomutase YP_666314.1 121833 D 393115 CDS YP_666315.1 110669758 4199345 123040..123801 1 NC_008245.1 Similar to AAP95774 (Q7VMS9) Deoxyribose-phosphate aldolase from Haemophilus ducreyi (258 aa). FASTA: opt: 632 Z-score: 764.1 E(): 1.1e-34 Smith-Waterman score: 632; 42.857 identity in 245 aa overlap; deoxyribose-phosphate aldolase 123801 deoC 4199345 deoC Francisella tularensis subsp. tularensis FSC198 deoxyribose-phosphate aldolase YP_666315.1 123040 D 393115 CDS YP_666316.1 110669759 4200754 123791..124990 1 NC_008245.1 Similar to AAP07626 (Q81I17) Nucleoside permease nupC from Bacillus cereus (392 aa). FASTA: opt: 1236 Z-score: 1430.7 E(): 7.7e-72 Smith-Waterman score: 1236; 49.246 identity in 398 aa overlap Paralog of FTF0116; nucleoside permease NUP family protein 124990 nupC1 4200754 nupC1 Francisella tularensis subsp. tularensis FSC198 nucleoside permease NUP family protein YP_666316.1 123791 D 393115 CDS YP_666317.1 110669760 4200755 125009..126211 1 NC_008245.1 Similar to (AAP07626) Q81I17 Nucleoside permease nupC from Bacillus cereus (392 aa). FASTA: opt: 1351 Z-score: 1549.9 E(): 1.9e-78 Smith-Waterman score: 1351; 52.381 identity in 399 aa overlap Paralog of FTF0115; nucleoside permease NUP family protein 126211 nupC1 4200755 nupC1 Francisella tularensis subsp. tularensis FSC198 nucleoside permease NUP family protein YP_666317.1 125009 D 393115 CDS YP_666318.1 110669761 4200756 126224..126853 1 NC_008245.1 catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP); thymidylate kinase 126853 tmk 4200756 tmk Francisella tularensis subsp. tularensis FSC198 thymidylate kinase YP_666318.1 126224 D 393115 CDS YP_666319.1 110669762 4199939 126915..128492 1 NC_008245.1 stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein; peptide chain release factor 3 128492 prfC 4199939 prfC Francisella tularensis subsp. tularensis FSC198 peptide chain release factor 3 YP_666319.1 126915 D 393115 CDS YP_666320.1 110669763 4200542 128567..129883 1 NC_008245.1 ORF ftt0119; hypothetical protein 129883 4200542 FTF0119 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666320.1 128567 D 393115 CDS YP_666321.1 110669764 4199198 129880..130911 1 NC_008245.1 Similar to Q9I6C1 Signal recognition particle receptor FtsY from Pseudomonas aeruginosa (455 aa). FASTA: opt: 1262 Z-score: 1403.4 E(): 2.8e-70 Smith-Waterman score: 1262; 61.935identity in 310 aa overlap; signal recognition particle receptor FtsY 130911 ftsY 4199198 ftsY Francisella tularensis subsp. tularensis FSC198 signal recognition particle receptor FtsY YP_666321.1 129880 D 393115 CDS YP_666322.1 110669765 4200136 131002..133224 1 NC_008245.1 Similar to Q88C31 DNA helicase II from Pseudomonas putida (728 aa). FASTA: opt: 1880 Z-score: 2129.9 E(): 9.6e-111 Smith-Waterman score: 2083; 45.867 identity in 750 aa overlap; DNA helicase II 133224 uvrD 4200136 uvrD Francisella tularensis subsp. tularensis FSC198 DNA helicase II YP_666322.1 131002 D 393115 CDS YP_666323.1 110669766 4200084 136649..137617 1 NC_008245.1 Similar to Q8D8A2 Oligopeptide ABC transporter,ATP-binding protein D from Vibrio vulnificus (324 aa). FASTA: opt: 1202 Z-score: 1353.8 E(): 1.6e-67 Smith-Waterman score: 1202; 56.270 identity in 311 aa overlap; oligopeptide transporter, subunit D, ABC transporter ATP-binding protein 137617 oppD 4200084 oppD Francisella tularensis subsp. tularensis FSC198 oligopeptide transporter, subunit D, ABC transporter ATP-binding protein YP_666323.1 136649 D 393115 CDS YP_666324.1 110669767 4200085 137618..138592 1 NC_008245.1 Similar to OPPF_HAEIN (P45051) Oligopeptide transport ATP-binding oppF from Haemophilus influenzae (332 aa). FASTA: opt: 1227 Z-score: 1398.5 E(): 5.3e-70 Smith-Waterman score: 1227; 56.790 identity in 324 aa overlap; oligopeptide transporter, subunit F, ABC transporter ATP-binding protein 138592 oppF 4200085 oppF Francisella tularensis subsp. tularensis FSC198 oligopeptide transporter, subunit F, ABC transporter ATP-binding protein YP_666324.1 137618 D 393115 CDS YP_666325.1 110669768 4200543 complement(138596..139798) 1 NC_008245.1 Similar to Q83CF6 Major facilitator family transporter from Coxiella burnetii (443 aa). FASTA: opt: 488 Z-score: 542.0 E(): 2.7e-22 Smith-Waterman score: 490; 27.621identity in 391 aa overlap ORF ftt0127c; major facilitator transporter 139798 4200543 FTF0127c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666325.1 138596 R 393115 CDS YP_666326.1 110669769 4200544 139932..140513 1 NC_008245.1 ORF ftt0128; hypothetical protein 140513 4200544 FTF0128 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666326.1 139932 D 393115 CDS YP_666327.1 110669770 4200545 140657..141925 1 NC_008245.1 Similar to Q83BM0 (Q83BM0) Major facilitator family transporter from Coxiella burnetii (428 aa). FASTA: opt: 497 Z-score: 533.3 E(): 8.2e-22 Smith-Waterman score: 529; 26.355 identity in 406 aa overlap ORF ftt0129; major facilitator transporter 141925 4200545 FTF0129 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666327.1 140657 D 393115 CDS YP_666328.1 110669771 4199796 141982..143490 1 NC_008245.1 Similar to GLPK_THETN (Q8R8J4) Glycerol kinase from Thermoanaerobacter tengcongensis (497 aa). FASTA: opt: 2233 Z-score: 2691.0 E(): 5.3e-142 Smith-Waterman score: 2233; 66.333 identity in 499 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; glycerol kinase 143490 glpK 4199796 glpK Francisella tularensis subsp. tularensis FSC198 glycerol kinase YP_666328.1 141982 D 393115 CDS YP_666329.1 110669772 4199617 complement(join(143583..144041,144041..144367)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 144367 isftu1 4199617 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666329.1 143583 R 393115 CDS YP_666330.1 110669773 4199793 144683..146215 1 NC_008245.1 Similar to O51259 Glycerol-3-phosphate dehydrogenase, anaerobic, from Borrelia burgdorferii (527 aa). FASTA: opt: 1330 Z-score: 1506.9 E(): 4.8e-76 Smith-Waterman score: 1330; 43.333 identity in 510 aa overlap; anaerobic glycerol-3-phosphate dehydrogenase 146215 glpA 4199793 glpA Francisella tularensis subsp. tularensis FSC198 anaerobic glycerol-3-phosphate dehydrogenase YP_666330.1 144683 D 393115 CDS YP_666331.1 110669774 4199795 146225..146989 1 NC_008245.1 Similar to CAD75967 (Q7UMF0) Probable glycerol uptake facilitator protein from Rhodopirelulla baltica (271 aa). FASTA: opt: 796 Z-score: 888.1 E(): 1.4e-41 Smith-Waterman score: 796; 50.209 identity in 239 aa overlap; glycerol uptake facilitator protein 146989 glpF 4199795 glpF Francisella tularensis subsp. tularensis FSC198 glycerol uptake facilitator protein YP_666331.1 146225 D 393115 CDS YP_666332.1 110669775 4200546 146993..147706 1 NC_008245.1 ORF ftt0134; hypothetical protein 147706 4200546 FTF0134 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666332.1 146993 D 393115 CDS YP_666333.1 110669776 4200548 148181..149596 1 NC_008245.1 Similar to AAQ00172 (Q7VBG7) DNA helicase related to phage enzyme from Prochlorococcus marinus (479 aa) FASTA: opt: 386 Z-score: 446.1 E(): 5.9e-17 Smith-Waterman score: 386; 26.681 identity in 476 aa overlap ORF ftt0136; helicase 149596 4200548 FTF0136 Francisella tularensis subsp. tularensis FSC198 helicase YP_666333.1 148181 D 393115 CDS YP_666334.1 110669777 4198974 150061..151245 1 NC_008245.1 EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu; elongation factor Tu 151245 tufA 4198974 tufA Francisella tularensis subsp. tularensis FSC198 elongation factor Tu YP_666334.1 150061 D 393115 CDS YP_666335.1 110669778 4200737 151437..151907 1 NC_008245.1 forms a complex with SecY and SecG; SecYEG forms a putative protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force; preprotein translocase subunit SecE 151907 secE 4200737 secE Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecE YP_666335.1 151437 D 393115 CDS YP_666336.1 110669779 4200759 151923..152456 1 NC_008245.1 Similar to NUSG_VIBVU (Q8DD25) Transcription antitermination protein nusG from Vibrio vulnificus (181 aa). FASTA: opt: 687 Z-score: 868.9 E(): 1.7e-40 Smith-Waterman score: 687; 58.621 identity in 174 aa overlap; transcription antitermination protein nusG 152456 nusG 4200759 nusG Francisella tularensis subsp. tularensis FSC198 transcription antitermination protein nusG YP_666336.1 151923 D 393115 CDS YP_666337.1 110669780 4200781 152525..152959 1 NC_008245.1 binds directly to 23S ribosomal RNA; 50S ribosomal protein L11 152959 rplK 4200781 rplK Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L11 YP_666337.1 152525 D 393115 CDS YP_666338.1 110669781 4200773 152962..153657 1 NC_008245.1 in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA; 50S ribosomal protein L1 153657 rplA 4200773 rplA Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L1 YP_666338.1 152962 D 393115 CDS YP_666339.1 110669782 4200780 153827..154345 1 NC_008245.1 binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit; 50S ribosomal protein L10 154345 rplJ 4200780 rplJ Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L10 YP_666339.1 153827 D 393115 CDS YP_666340.1 110669783 4200782 154408..154785 1 NC_008245.1 present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors; 50S ribosomal protein L7/L12 154785 rplL 4200782 rplL Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L7/L12 YP_666340.1 154408 D 393115 CDS YP_666341.1 110669784 4200328 154939..159015 1 NC_008245.1 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme; DNA-directed RNA polymerase subunit beta 159015 rpoB 4200328 rpoB Francisella tularensis subsp. tularensis FSC198 DNA-directed RNA polymerase subunit beta YP_666341.1 154939 D 393115 CDS YP_666342.1 110669785 4200329 159077..163330 1 NC_008245.1 Similar to Q9AAU1 (Q9AAU1) DNA-directed RNA polymerase, beta subunit from Caulobacter crescentus (1396 aa). FASTA: opt: 5089 Z-score: 5550.7 E(): 0 Smith-Waterman score: 5089; 56.626identity in 1381 aa overlap; DNA-directed RNA polymerase subunit beta 163330 rpoC 4200329 rpoC Francisella tularensis subsp. tularensis FSC198 DNA-directed RNA polymerase subunit beta YP_666342.1 159077 D 393115 CDS YP_666343.1 110669786 4200549 163433..163669 1 NC_008245.1 ORF ftt0146; hypothetical protein 163669 4200549 FTF0146 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666343.1 163433 D 393115 CDS YP_666344.1 110669787 4199276 163669..164679 1 NC_008245.1 in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity; putative DNA-binding/iron metalloprotein/AP endonuclease 164679 gcp 4199276 gcp Francisella tularensis subsp. tularensis FSC198 putative DNA-binding/iron metalloprotein/AP endonuclease YP_666344.1 163669 D 393115 CDS YP_666345.1 110669788 4200550 164793..165959 1 NC_008245.1 Similar to Q27437 Stearoyl-CoA desaturase from Amblyomma americanum (317 aa). FASTA: opt: 653 Z-score: 747.5 E(): 9.6e-34 Smith-Waterman score: 653; 36.897 identity in 290 aa overlap ORF ftt0148; fatty acid desaturase 165959 4200550 FTF0148 Francisella tularensis subsp. tularensis FSC198 fatty acid desaturase YP_666345.1 164793 D 393115 CDS YP_666346.1 110669789 4199839 complement(166171..167331) 1 NC_008245.1 catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase; S-adenosylmethionine synthetase 167331 metK 4199839 metK Francisella tularensis subsp. tularensis FSC198 S-adenosylmethionine synthetase YP_666346.1 166171 R 393115 CDS YP_666347.1 110669790 4198954 167625..167873 1 NC_008245.1 binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity; 30S ribosomal protein S16 167873 rpsP 4198954 rpsP Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S16 YP_666347.1 167625 D 393115 CDS YP_666348.1 110669791 4199220 167916..168425 1 NC_008245.1 Essential for efficient processing of 16S rRNA; 16S rRNA-processing protein RimM 168425 rimM 4199220 rimM Francisella tularensis subsp. tularensis FSC198 16S rRNA-processing protein RimM YP_666348.1 167916 D 393115 CDS YP_666349.1 110669792 4199863 168822..169586 1 NC_008245.1 methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA; tRNA (guanine-N(1)-)-methyltransferase 169586 trmD 4199863 trmD Francisella tularensis subsp. tularensis FSC198 tRNA (guanine-N(1)-)-methyltransferase YP_666349.1 168822 D 393115 CDS YP_666350.1 110669793 4198995 169586..169933 1 NC_008245.1 this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site; 50S ribosomal protein L19 169933 rplS 4198995 rplS Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L19 YP_666350.1 169586 D 393115 CDS YP_666351.1 110669794 4200694 170099..170977 1 NC_008245.1 Similar to Q7ZAJ8 (Q7ZAJ8) Integrase/recombinase XerD from Shewanella oneidensis (300 aa). FASTA: opt: 1039 Z-score: 1285.0 E(): 1.1e-63 Smith-Waterman score: 1039; 52.414 identity in 290 aa overlap; integrase/recombinase 170977 xerD 4200694 xerD Francisella tularensis subsp. tularensis FSC198 integrase/recombinase YP_666351.1 170099 D 393115 CDS YP_666352.1 110669795 4200551 171094..171945 1 NC_008245.1 Similar to Q87HU0 Putative oxidoreductase iron/ascorbate from Vibrio parahaemolyticus (279 aa). FASTA: opt: 944 Z-score: 1155.0 E(): 1.9e-56 Smith-Waterman score: 944; 49.110 identity in 281 aa overlap ORF ftt0155; oxidoreductase iron/ascorbate family protein 171945 4200551 FTF0155 Francisella tularensis subsp. tularensis FSC198 oxidoreductase iron/ascorbate family protein YP_666352.1 171094 D 393115 CDS YP_666353.1 110669796 4200630 171950..172534 1 NC_008245.1 Similar to Q83EI5 Acid phosphatase, class B from Coxiella burnetti (221 aa). FASTA: opt: 326 Z-score: 430.9 E(): 4.1e-16 Smith-Waterman score: 326; 32.642identity in 193 aa overlap ORF ftt0156; acid phosphatase 172534 4200630 FTF0156 Francisella tularensis subsp. tularensis FSC198 acid phosphatase YP_666353.1 171950 D 393115 CDS YP_666354.1 110669797 4200631 complement(172531..173415) 1 NC_008245.1 Similar to Q8XMR0 Probable lic-1 operon protein from Clostridium perfringens (322 aa). FASTA: opt: 370 Z-score: 410.4 E(): 5.7e-15 Smith-Waterman score: 396; 30.492 identity in 305 aa overlap ORF ftt0157c; licB-like transmembrane protein 173415 4200631 FTF0157c Francisella tularensis subsp. tularensis FSC198 licB-like transmembrane protein YP_666354.1 172531 R 393115 CDS YP_666355.1 110669798 4200632 complement(173547..174155) 1 NC_008245.1 ORF ftt0158c; hypothetical protein 174155 4200632 FTF0158c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666355.1 173547 R 393115 CDS YP_666356.1 110669799 4200633 complement(174232..175053) 1 NC_008245.1 ORF ftt0159c; hypothetical protein 175053 4200633 FTF0159c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666356.1 174232 R 393115 CDS YP_666357.1 110669800 4200244 175216..175683 1 NC_008245.1 hydrolyzes diadenosine polyphosphate; dinucleoside polyphosphate hydrolase 175683 nudH 4200244 nudH Francisella tularensis subsp. tularensis FSC198 dinucleoside polyphosphate hydrolase YP_666357.1 175216 D 393115 CDS YP_666358.1 110669801 4200634 complement(175693..176319) 1 NC_008245.1 Similar to Q8FXE8 PAP2 family protein from Brucella suis (325 aa). FASTA: opt: 314 Z-score: 375.8 E(): 4.8e-13 Smith-Waterman score: 314; 31.915identity in 188 aa overlap ORF ftt0161c; PAP2 family protein 176319 4200634 FTF0161c Francisella tularensis subsp. tularensis FSC198 PAP2 family protein YP_666358.1 175693 R 393115 CDS YP_666359.1 110669802 4199421 176393..176917 1 NC_008245.1 Similar to Q88PQ9 N-acetyl-anhydromuramyl-L-alanine amidase ampD from Pseudomonas putida (190 aa). FASTA: opt: 623 Z-score: 809.4 E(): 3.4e-37 Smith-Waterman score: 623; 52.000 identity in 175 aa overlap; N-acetyl-anhydromuranmyl-L-alanine amidase 176917 ampD 4199421 ampD Francisella tularensis subsp. tularensis FSC198 N-acetyl-anhydromuranmyl-L-alanine amidase YP_666359.1 176393 D 393115 CDS YP_666360.1 110669803 4200096 complement(176975..178858) 1 NC_008245.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit B 178858 parE 4200096 parE Francisella tularensis subsp. tularensis FSC198 DNA topoisomerase IV subunit B YP_666360.1 176975 R 393115 CDS YP_666361.1 110669804 4200635 complement(178948..180129) 1 NC_008245.1 Similar to Q9KRK6 Multidrug resistance protein from Vibrio cholerae (401 aa). FASTA: opt: 579 Z-score: 614.0 E(): 2.6e-26 Smith-Waterman score: 579; 28.989identity in 376 aa overlap ORF ftt0164c; eflux protein 180129 4200635 FTF0164c Francisella tularensis subsp. tularensis FSC198 eflux protein YP_666361.1 178948 R 393115 CDS YP_666362.1 110669805 4200636 complement(180129..181499) 1 NC_008245.1 Similar to Q88PJ4 Conserved hypothetical protein from Pseudomonas putida (380 aa). FASTA: opt: 644 Z-score: 755.3 E(): 3.5e-34 Smith-Waterman score: 646; 29.268 identity in 369 aa overlap ORF ftt0165c; lipoprotein 181499 4200636 FTF0165c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666362.1 180129 R 393115 CDS YP_666363.1 110669806 4200637 complement(181502..182158) 1 NC_008245.1 Similar to Q8FF57 Hypothetical protein yfgM from E. coli (206 aa). FASTA: opt: 155 Z-score: 188.7 E(): 0.012 Smith-Waterman score: 188; 27.778 identity in 198 aa overlap ORF ftt0166c; hypothetical protein 182158 4200637 FTF0166c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666363.1 181502 R 393115 CDS YP_666364.1 110669807 4200370 182339..183589 1 NC_008245.1 catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins; glutamyl-tRNA reductase 183589 hemA 4200370 hemA Francisella tularensis subsp. tularensis FSC198 glutamyl-tRNA reductase YP_666364.1 182339 D 393115 CDS YP_666365.1 110669808 4199937 183590..184675 1 NC_008245.1 recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2; peptide chain release factor 1 184675 prfA 4199937 prfA Francisella tularensis subsp. tularensis FSC198 peptide chain release factor 1 YP_666365.1 183590 D 393115 CDS YP_666366.1 110669809 4200638 184668..185522 1 NC_008245.1 ORF ftt0169; hemK protein 185522 4200638 FTF0169 Francisella tularensis subsp. tularensis FSC198 hemK protein YP_666366.1 184668 D 393115 CDS YP_666367.1 110669810 4199618 join(186007..186333,186333..186791) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 186791 isftu1 4199618 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666367.1 186007 D 393115 CDS YP_666368.1 110669811 4200642 188232..188801 1 NC_008245.1 Similar to Q83A22 Hypothetical protein from Coxiella burnetti (192 aa). FASTA: opt: 440 Z-score: 543.8 E(): 2.1e-22 Smith-Waterman score: 440; 37.634 identity in 186 aa overlap ORF ftt0174; YggT family protein 188801 4200642 FTF0174 Francisella tularensis subsp. tularensis FSC198 YggT family protein YP_666368.1 188232 D 393115 CDS YP_666369.1 110669812 4200643 complement(188795..189577) 1 NC_008245.1 Similar to AAO80856 (Q836Q5) ABC transporter,ATP-binding protein (269 aa). FASTA: opt: 738 Z-score: 809.5 E(): 3.1e-37 Smith-Waterman score: 738; 43.137 identity in 255 aa overlap missing 90 aa at C-terminus ORF ftt0175c; ABC transporter ATP-binding protein 189577 4200643 FTF0175c Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_666369.1 188795 R 393115 CDS YP_666370.1 110669813 4200645 complement(191761..192186) 1 NC_008245.1 Similar to Q88IP2 Acetyltransferase, GNAT family from Pseudomonas putida (364 aa). FASTA: opt: 250 Z-score: 324.7 E(): 3.1e-10 Smith-Waterman score: 250; 34.559 identity in 136 aa overlap ORF ftt0177c; acetyltransferase 192186 4200645 FTF0177c Francisella tularensis subsp. tularensis FSC198 acetyltransferase YP_666370.1 191761 R 393115 CDS YP_666371.1 110669814 4199219 complement(192173..193642) 1 NC_008245.1 Similar to Q9KPN3 Ribosomal protein S6 modification protein-related protein from Vibrio cholerae (483 aa). FASTA: opt: 1367 Z-score: 1648.7 E(): 6.1e-84 Smith-Waterman score: 1367; 43.149 identity in 489 aa overlap; 30S ribosomal protein S6 193642 rimK 4199219 rimK Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S6 YP_666371.1 192173 R 393115 CDS YP_666372.1 110669815 4200647 195923..196813 1 NC_008245.1 Similar to Q8DDF5 1-acyl-sn-glycerol-3-phosphate acyltransferase from Vibrio vulnificus (271 aa). FASTA: opt: 749 Z-score: 902.5 E(): 2e-42 Smith-Waterman score: 749; 40.370 identity in 270 aa overlap ORF ftt0180; putative acyltransferase 196813 4200647 FTF0180 Francisella tularensis subsp. tularensis FSC198 putative acyltransferase YP_666372.1 195923 D 393115 CDS YP_666373.1 110669816 4200783 complement(197210..197746) 1 NC_008245.1 Similar to Q9X885 Putative small integral membrane protein from Streptomyces coelicolor (167 aa). FASTA: opt: 262 Z-score: 342.1 E(): 3.6e-11 Smith-Waterman score: 262; 30.723 identity in 166 aa overlap ORF ftt0181c; hypothetical protein 197746 4200783 FTF0181c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666373.1 197210 R 393115 CDS YP_666374.1 110669817 4200784 complement(197844..198386) 1 NC_008245.1 Similar to Q8D257 YrdC protein from Wigglesworthia glossinidia (194 aa). FASTA: opt: 374 Z-score: 473.0 E(): 1.9e-18 Smith-Waterman score: 374; 39.130 identity in 184 aa overlap ORF ftt0182c; Sua5/YciO/YrdC family protein 198386 4200784 FTF0182c Francisella tularensis subsp. tularensis FSC198 Sua5/YciO/YrdC family protein YP_666374.1 197844 R 393115 CDS YP_666375.1 110669818 4200333 complement(198503..200173) 1 NC_008245.1 in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins; 30S ribosomal protein S1 200173 rpsA 4200333 rpsA Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S1 YP_666375.1 198503 R 393115 CDS YP_666376.1 110669819 4200785 200321..200782 1 NC_008245.1 Similar to Q83DS3 Zinc-binding domain protein from Coxiella burnetii (142 aa). FASTA: opt: 473 Z-score: 642.4 E(): 6.8e-28 Smith-Waterman score: 473; 52.817 identity in 142 aa overlap ORF ftt0184; zinc-binding domain-containing protein 200782 4200785 FTF0184 Francisella tularensis subsp. tularensis FSC198 zinc-binding domain-containing protein YP_666376.1 200321 D 393115 CDS YP_666377.1 110669820 4199530 200786..201676 1 NC_008245.1 D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli; D-alanyl-alanine synthetase A 201676 ddlB 4199530 ddlB Francisella tularensis subsp. tularensis FSC198 D-alanyl-alanine synthetase A YP_666377.1 200786 D 393115 CDS YP_666378.1 110669821 4199196 201673..202356 1 NC_008245.1 Similar to Q83F15 Cell division protein FtsQ from Coxiella burnetii (243 aa). FASTA: opt: 291 Z-score: 355.4 E(): 6.6e-12 Smith-Waterman score: 291; 23.176 identity in 233 aa overlap; cell division protein FtsQ 202356 ftsQ 4199196 ftsQ Francisella tularensis subsp. tularensis FSC198 cell division protein FtsQ YP_666378.1 201673 D 393115 CDS YP_666379.1 110669822 4199192 202458..203720 1 NC_008245.1 Similar to Q87WY9 Cell division protein FtsA from Pseuodomonas syringae (418 aa). FASTA: opt: 992 Z-score: 1194.6 E(): 1.2e-58 Smith-Waterman score: 992; 37.621 identity in 412 aa overlap; cell division protein FtsA 203720 ftsA 4199192 ftsA Francisella tularensis subsp. tularensis FSC198 cell division protein FtsA YP_666379.1 202458 D 393115 CDS YP_666380.1 110669823 4199199 203764..204909 1 NC_008245.1 GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function; cell division protein FtsZ 204909 ftsZ 4199199 ftsZ Francisella tularensis subsp. tularensis FSC198 cell division protein FtsZ YP_666380.1 203764 D 393115 CDS YP_666381.1 110669824 4199571 204932..205792 1 NC_008245.1 zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase 205792 lpxC 4199571 lpxC Francisella tularensis subsp. tularensis FSC198 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase YP_666381.1 204932 D 393115 CDS YP_666382.1 110669825 4199359 complement(205806..207446) 1 NC_008245.1 Similar to Q8DB24 DNA polymerase III, gamma/tau subunits from Vibrio vulnificus (734 aa). FASTA: opt: 1193 Z-score: 1334.5 E(): 1.9e-66 Smith-Waterman score: 1193; 44.893 identity in 421 aa overlap; DNA polymerase III subunit gamma and tau 207446 dnaX 4199359 dnaX Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit gamma and tau YP_666382.1 205806 R 393115 CDS YP_666383.1 110669826 4199938 207719..208696 1 NC_008245.1 Similar to RF2_HAEIN (P43918) Peptide chain release factor 2 from Haemophilus influenzae (365 aa). FASTA: opt: 1542 Z-score: 1839.9 E(): 1.4e-94 Smith-Waterman score: 1542; 68.405 identity in 326 aa overlap; peptide chain release factor 2 208696 prfB 4199938 prfB Francisella tularensis subsp. tularensis FSC198 peptide chain release factor 2 YP_666383.1 207719 D 393115 CDS YP_666384.1 110669827 4199655 208829..210559 1 NC_008245.1 Similar to SYK2_ECOLI (P14825) Lysyl-tRNA synthetase, heat inducible, from E. coli (504 aa). FASTA: opt: 2003 Z-score: 2242.9 E(): 4.9e-117 Smith-Waterman score: 2003; 61.066 identity in 488 aa overlap; lysyl-tRNA synthetase 210559 lysU 4199655 lysU Francisella tularensis subsp. tularensis FSC198 lysyl-tRNA synthetase YP_666384.1 208829 D 393115 CDS YP_666385.1 110669828 4200786 complement(210944..211486) 1 NC_008245.1 Similar to Y208_VIBCH (Q9KVD8) Hypothetical protein VC0208 from Vibrio cholerae (162 aa). FASTA: opt: 414 Z-score: 528.5 E(): 1.5e-21 Smith-Waterman score: 414; 41.875 identity in 160 aa overlap ORF ftt0193c; hypothetical protein 211486 4200786 FTF0193c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666385.1 210944 R 393115 CDS YP_666386.1 110669829 4200787 complement(211585..212457) 1 NC_008245.1 Similar to Q8X898 Hypothetical 31.1 kDa protein (Hypothetical membrane protein) from E.coli (274 aa). FASTA: opt: 398 Z-score: 431.1 E(): 4e-16 Smith-Waterman score: 398; 26.429 identity in 280 aa overlap ORF ftt0194c; hypothetical protein 212457 4200787 FTF0194c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666386.1 211585 R 393115 CDS YP_666387.1 110669830 4200788 complement(212466..214007) 1 NC_008245.1 Similar to Q8IX91 L-glutaminase from Homo sapien (602 aa). FASTA: opt: 1441 Z-score: 1670.0 E(): 3.9e-85 Smith-Waterman score: 1441; 42.887 identity in 478 aa overlap ORF ftt0195c; L-glutaminase 214007 4200788 FTF0195c Francisella tularensis subsp. tularensis FSC198 L-glutaminase YP_666387.1 212466 R 393115 CDS YP_666388.1 110669831 4199789 complement(214089..215126) 1 NC_008245.1 Similar to AAO90051 (Q83E31) Glutamine synthetase from Coxiella burnetti (359 aa). FASTA: opt: 1369 Z-score: 1705.1 E(): 4.4e-87 Smith-Waterman score: 1369; 59.226 identity in 336 aa overlap; glutamine synthetase 215126 glnA 4199789 glnA Francisella tularensis subsp. tularensis FSC198 glutamine synthetase YP_666388.1 214089 R 393115 CDS YP_666389.1 110669832 4200197 complement(215246..216223) 1 NC_008245.1 Similar to Q88DM9 DNA polymerase III, delta subunit from Pseudomonas putida (345 aa). FASTA: opt: 380 Z-score: 451.8 E(): 2.8e-17 Smith-Waterman score: 380; 28.707 identity in 317 aa overlap; DNA polymerase III subunit delta 216223 holA 4200197 holA Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit delta YP_666389.1 215246 R 393115 CDS YP_666390.1 110669833 4200311 216351..216827 1 NC_008245.1 Similar to Q8EGB4 Lipoprotein Blc from Shewenella oneidensis (177 aa). FASTA: opt: 620 Z-score: 813.1 E(): 2.1e-37 Smith-Waterman score: 620; 56.604 identity in 159 aa overlap; outer membrane lipoprotein 216827 blc 4200311 blc Francisella tularensis subsp. tularensis FSC198 outer membrane lipoprotein YP_666390.1 216351 D 393115 CDS YP_666391.1 110669834 4200789 216991..217824 1 NC_008245.1 ORF ftt0199; hypothetical protein 217824 4200789 FTF0199 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666391.1 216991 D 393115 CDS YP_666392.1 110669835 4200790 217772..218389 1 NC_008245.1 ORF ftt0200; hypothetical protein 218389 4200790 FTF0200 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666392.1 217772 D 393115 CDS YP_666393.1 110669836 4200190 complement(218963..219706) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 219706 isftu2 4200190 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666393.1 218963 R 393115 CDS YP_666394.1 110669837 4199119 complement(220957..222504) 1 NC_008245.1 involved in de novo purine biosynthesis; bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase 222504 purH 4199119 purH Francisella tularensis subsp. tularensis FSC198 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase YP_666394.1 220957 R 393115 CDS YP_666395.1 110669838 4199951 222681..223967 1 NC_008245.1 catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis; adenylosuccinate synthetase 223967 purA 4199951 purA Francisella tularensis subsp. tularensis FSC198 adenylosuccinate synthetase YP_666395.1 222681 D 393115 CDS YP_666396.1 110669839 4200200 223971..224504 1 NC_008245.1 Similar to Q8R7L0 Hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcogenesis (181 aa). FASTA: opt: 509 Z-score: 658.7 E(): 8.5e-29 Smith-Waterman score: 509; 47.337 identity in 169 aa overlap; hypoxanthine-guanine phosphoribosyltransferase 224504 hpt 4200200 hpt Francisella tularensis subsp. tularensis FSC198 hypoxanthine-guanine phosphoribosyltransferase YP_666396.1 223971 D 393115 CDS YP_666397.1 110669840 4200793 complement(225339..226181) 1 NC_008245.1 Similar to Q8G5L3 Possible permease of ABC transporter system from Bifidobacterium longum (277 aa). FASTA: opt: 582 Z-score: 622.6 E(): 8.7e-27 Smith-Waterman score: 582; 38.372identity in 258 aa overlap ORF ftt0207c; ABC transporter permease 226181 4200793 FTF0207c Francisella tularensis subsp. tularensis FSC198 ABC transporter permease YP_666397.1 225339 R 393115 CDS YP_666398.1 110669841 4200794 complement(226123..226800) 1 NC_008245.1 Similar to MNTB_BACSU (O34338) Manganese transport system ATP-binding protein mntB from Bacillus subtilis (250 aa). FASTA: opt: 365 Z-score: 426.7 E(): 7.1e-16 Smith-Waterman score: 365; 30.986 identity in 213 aa overlap ORF ftt0208c; ABC transporter ATP-binding protein 226800 4200794 FTF0208c Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_666398.1 226123 R 393115 CDS YP_666399.1 110669842 4200795 complement(226802..227719) 1 NC_008245.1 Similar to Q88TE8 ABC transporter substrate binding protein from Lactobacillus plantarum (297 aa). FASTA: opt: 555 Z-score: 628.8 E(): 3.9e-27 Smith-Waterman score: 555; 30.935identity in 278 aa overlap ORF ftt0209c; periplasmic solute binding family protein 227719 4200795 FTF0209c Francisella tularensis subsp. tularensis FSC198 periplasmic solute binding family protein YP_666399.1 226802 R 393115 CDS YP_666400.1 110669843 4200797 complement(229135..229599) 1 NC_008245.1 Similar to Q9F6B1 Outer membrane lipoprotein Pcp from Edwardsiella tarda (155 aa). FASTA: opt: 294 Z-score: 329.0 E(): 2e-10 Smith-Waterman score: 294; 35.032 identity in 157 aa overlap ORF ftt0211c; outer membrane lipoprotein 229599 4200797 FTF0211c Francisella tularensis subsp. tularensis FSC198 outer membrane lipoprotein YP_666400.1 229135 R 393115 CDS YP_666401.1 110669844 4200688 complement(229610..230206) 1 NC_008245.1 Similar to Q9I509 Trp repressor binding protein WrbA from Pseudomonas aeruginosa (198 aa). FASTA: opt: 727 Z-score: 948.0 E(): 6.5e-45 Smith-Waterman score: 727; 54.974 identity in 191 aa overlap; trp repressor binding protein 230206 wrbA 4200688 wrbA Francisella tularensis subsp. tularensis FSC198 trp repressor binding protein YP_666401.1 229610 R 393115 CDS YP_666402.1 110669845 4200597 230213..231400 1 NC_008245.1 Similar to RBN_ECOLI (P32146) tRNA processing ribonuclease BN from E. coli (290 aa). FASTA: opt: 535 Z-score: 578.5 E(): 2.5e-24 Smith-Waterman score: 535; 33.813 identity in 278 aa overlap; tRNA processing ribonuclease BN 231400 rbn 4200597 rbn Francisella tularensis subsp. tularensis FSC198 tRNA processing ribonuclease BN YP_666402.1 230213 D 393115 CDS YP_666403.1 110669846 4200798 231419..231976 1 NC_008245.1 Similar to Q9I2E6 Probable transporter from Pseudomonas aeruginosa (191 aa). FASTA: Q8PGT5 PnuC protein from Xanthomonas axonopodois (191 aa). FASTA: opt: 216 Z-score: 281.0 E(): 8.5e-08 Smith-Waterman score: 216; 27.072 identity in 181 aa overlap. Contains a frameshift in Schu S4 but intact in FSC 198; transporter 231976 4200798 FTF0214 Francisella tularensis subsp. tularensis FSC198 transporter YP_666403.1 231419 D 393115 CDS YP_666404.1 110669847 4199940 231990..234143 1 NC_008245.1 Similar to Q9KNQ3 Primosomal protein N` from Vibrio cholerae (734 aa). FASTA: opt: 1587 Z-score: 1770.8 E(): 9.7e-91 Smith-Waterman score: 1619; 37.823 identity in 735 aa overlap; primosomal protein N' 234143 priA 4199940 priA Francisella tularensis subsp. tularensis FSC198 primosomal protein N' YP_666404.1 231990 D 393115 CDS YP_666405.1 110669848 4199619 join(234273..234599,234599..235057) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 235057 isftu1 4199619 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666405.1 234273 D 393115 CDS YP_666406.1 110669849 4200343 complement(235996..236991) 1 NC_008245.1 Similar to Q81FW2 Low-affinity inorganic phosphate transport protein from Bacillus cereus (332 aa). FASTA: opt: 932 Z-score: 1065.7 E(): 1.8e-51 Smith-Waterman score: 932; 42.470 identity in 332 aa overlap ORF ftt0219c; phosphate transport protein 236991 4200343 FTF0219c Francisella tularensis subsp. tularensis FSC198 phosphate transport protein YP_666406.1 235996 R 393115 CDS YP_666407.1 110669850 4200344 complement(237009..237665) 1 NC_008245.1 Similar to Q8XMV6 Hypothetical protein CPE0582 from Clostridium perfringens (210 aa). FASTA: opt: 207 Z-score: 248.6 E(): 5.9e-06 Smith-Waterman score: 207; 24.883 identity in 213 aa overlap ORF ftt0220c; hypothetical protein 237665 4200344 FTF0220c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666407.1 237009 R 393115 CDS YP_666408.1 110669851 4199753 237902..239446 1 NC_008245.1 acid phosphatase 239446 acpA 4199753 acpA Francisella tularensis subsp. tularensis FSC198 acid phosphatase YP_666408.1 237902 D 393115 CDS YP_666409.1 110669852 4200702 complement(239453..240229) 1 NC_008245.1 Similar to YBGK_HAEIN (P44298) Hypothetical protein HI1730 from Haemophilus influenzae (309 aa). FASTA: opt: 308 Z-score: 386.7 E(): 1.2e-13 Smith-Waterman score: 352; 32.453 identity in 265 aa overlap; hydrolase subunit 240229 ybgK 4200702 ybgK Francisella tularensis subsp. tularensis FSC198 hydrolase subunit YP_666409.1 239453 R 393115 CDS YP_666410.1 110669853 4200703 complement(240271..240969) 1 NC_008245.1 Similar to Y993_PYRAB (Q9V005) Hypothetical UPF0271 protein PYRAB09930 from Pyrococcus abyssi (255 aa). FASTA: opt: 430 Z-score: 541.0 E(): 3e-22 Smith-Waterman score: 430; 35.294 identity in 238 aa overlap; LamB/YcsF family protein 240969 ybgL 4200703 ybgL Francisella tularensis subsp. tularensis FSC198 LamB/YcsF family protein YP_666410.1 240271 R 393115 CDS YP_666411.1 110669854 4199620 complement(join(242754..243212,243212..243538)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 243538 isftu1 4199620 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666411.1 242754 R 393115 CDS YP_666412.1 110669855 4200347 complement(243562..243756) 1 NC_008245.1 ORF ftt0227c; hypothetical protein 243756 4200347 FTF0227c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666412.1 243562 R 393115 CDS YP_666413.1 110669856 4200086 complement(243791..244327) 1 NC_008245.1 3'-5' exoribonuclease specific for small oligoribonuclotides; oligoribonuclease 244327 orn 4200086 orn Francisella tularensis subsp. tularensis FSC198 oligoribonuclease YP_666413.1 243791 R 393115 CDS YP_666414.1 110669857 4199670 complement(244330..244899) 1 NC_008245.1 Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA; elongation factor P 244899 efp 4199670 efp Francisella tularensis subsp. tularensis FSC198 elongation factor P YP_666414.1 244330 R 393115 CDS YP_666415.1 110669858 4200348 complement(244939..245346) 1 NC_008245.1 Similar to Q87B50 Type IV pilin from Xylella fastidiosa (142 aa). FASTA: opt: 224 Z-score: 300.3 E(): 7.8e-09 Smith-Waterman score: 224; 32.090 identity in 134 aa overlap The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0230c; Type IV pili fiber building block protein 245346 4200348 FTF0230c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_666415.1 244939 R 393115 CDS YP_666416.1 110669859 4200649 complement(245359..246282) 1 NC_008245.1 Similar to HTRB_ECOLI (P24187) Lipid A biosynthesis lauroyl acyltransferase from E. coli (306 aa). FASTA: opt: 542 Z-score: 697.7 E(): 5.7e-31 Smith-Waterman score: 542; 32.895 identity in 304 aa overlap (8-307:14-306) n.b.Paralog of FTF0232c; acyltransferase 246282 htrB 4200649 htrB Francisella tularensis subsp. tularensis FSC198 acyltransferase YP_666416.1 245359 R 393115 CDS YP_666417.1 110669860 4199529 complement(246343..247242) 1 NC_008245.1 Similar to Q8ZNA3 (Q8ZNA3) Cold shock-induced palmitoleoyl transferase from Salmonella typhimurium (306 aa). FASTA: opt: 629 Z-score: 782.5 E(): 1.1e-35 Smith-Waterman score: 629; 36.755 identity in 302 aa overlap Paralog of FTF0231c; acyltransferase 247242 ddg 4199529 ddg Francisella tularensis subsp. tularensis FSC198 acyltransferase YP_666417.1 246343 R 393115 CDS YP_666418.1 110669861 4199108 complement(247249..248904) 1 NC_008245.1 Similar to 60IM_COXBU (P45650) 60 kDa inner-membrane protein homolog from Coxiella burnetti (566 aa). FASTA: opt: 1434 Z-score: 1537.8 E(): 8.4e-78 Smith-Waterman score: 1434; 41.815 identity in 562 aa overlap; inner-membrane protein 248904 yidC 4199108 yidC Francisella tularensis subsp. tularensis FSC198 inner-membrane protein YP_666418.1 247249 R 393115 CDS YP_666419.1 110669862 4200349 complement(248913..249125) 1 NC_008245.1 Similar to Q8RHA5 Hypothetical protein FN0003 from Fusobacterium nucleatum (82 aa). FASTA: opt: 273 Z-score: 406.9 E(): 8.2e-15 Smith-Waterman score: 273; 53.623 identity in 69 aa overlap ORF ftt0234c; hypothetical protein 249125 4200349 FTF0234c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666419.1 248913 R 393115 CDS YP_666420.1 110669863 4200768 complement(249137..249538) 1 NC_008245.1 Similar to RNPA_VIBCH (Q9KVY2) Ribonuclease P protein component from Vibrio cholerae (118 aa). FASTA: opt: 189 Z-score: 256.8 E(): 2.1e-06 Smith-Waterman score: 189; 35.870 identity in 92 aa overlap; ribonuclease P protein component 249538 rnpA 4200768 rnpA Francisella tularensis subsp. tularensis FSC198 ribonuclease P protein component YP_666420.1 249137 R 393115 CDS YP_666421.1 110669864 4199008 complement(249480..249614) 1 NC_008245.1 in Escherichia coli transcription of this gene is enhanced by polyamines; 50S ribosomal protein L34 249614 rpmH 4199008 rpmH Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L34 YP_666421.1 249480 R 393115 CDS YP_666422.1 110669865 4200350 complement(249706..250164) 1 NC_008245.1 ORF ftt0237c; hypothetical protein 250164 4200350 FTF0237c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666422.1 249706 R 393115 CDS YP_666423.1 110669866 4199435 250291..251064 1 NC_008245.1 Similar to AROE_VIBVU (Q8DDD4) Shikimate 5-dehydrogenase from Vibrio vulnificus (277 aa). FASTA: opt: 567 Z-score: 672.1 E(): 1.5e-29 Smith-Waterman score: 567; 37.984 identity in 258 aa overlap; shikimate 5-dehydrogenase 251064 aroE1 4199435 aroE1 Francisella tularensis subsp. tularensis FSC198 shikimate 5-dehydrogenase YP_666423.1 250291 D 393115 CDS YP_666424.1 110669867 4200106 251131..252486 1 NC_008245.1 Similar to Q9F1M9 MurC from Shewanella violacae (494 aa). FASTA: opt: 1010 Z-score: 1212.3 E(): 1.2e-59 Smith-Waterman score: 1010; 36.761 identity in 457 aa overlap; UDP-N-acetylmuramate--L-alanine ligase 252486 murC 4200106 murC Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylmuramate--L-alanine ligase YP_666424.1 251131 D 393115 CDS YP_666425.1 110669868 4200351 252479..253351 1 NC_008245.1 Similar to Q87SH3 Conserved hypothetical protein from Vibrio parahaemolyticus (287 aa). FASTA: opt: 744 Z-score: 882.7 E(): 2.8e-41 Smith-Waterman score: 744; 46.996 identity in 283 aa overlap ORF ftt0240; tetrapyrrole methyltransferase family protein 253351 4200351 FTF0240 Francisella tularensis subsp. tularensis FSC198 tetrapyrrole methyltransferase family protein YP_666425.1 252479 D 393115 CDS YP_666426.1 110669869 4200352 complement(253362..253832) 1 NC_008245.1 ORF ftt0241c; hypothetical protein 253832 4200352 FTF0241c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666426.1 253362 R 393115 CDS YP_666427.1 110669870 4200353 254172..254735 1 NC_008245.1 Similar to Q83DS9 Hypothetical protein CBU0616 from Coxiella burnetii (196 aa). FASTA: opt: 376 Z-score: 484.1 E(): 4.5e-19 Smith-Waterman score: 376; 37.634 identity in 186 aa overlap ORF ftt0242; hypothetical protein 254735 4200353 FTF0242 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666427.1 254172 D 393115 CDS YP_666428.1 110669871 4200354 254808..255587 1 NC_008245.1 weak similarity to M. musculus protein,1700056O17 Rik protein (233 aa) ORF ftt0243; hypothetical protein 255587 4200354 FTF0243 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666428.1 254808 D 393115 CDS YP_666429.1 110669872 4200355 255571..258312 1 NC_008245.1 Similar to Q8R5Z3 DNA/RNA helicase (DEAD/DEAH box family) from Fusobacterium nucleatum (942 aa). FASTA: opt: 2318 Z-score: 2654.5 E(): 5.8e-140 Smith-Waterman score: 2473; 45.087 identity in 916 aa overlap ORF ftt0244; DNA/RNA helicase 258312 4200355 FTF0244 Francisella tularensis subsp. tularensis FSC198 DNA/RNA helicase YP_666429.1 255571 D 393115 CDS YP_666430.1 110669873 4198986 258388..259224 1 NC_008245.1 Similar to Q8PWX4 Universal stress protein from Methanosarcina maizae (323 aa). FASTA: opt: 347 Z-score: 418.2 E(): 2.1e-15 Smith-Waterman score: 347; 31.849 identity in 292 aa overlap; universal stress protein 259224 usp 4198986 usp Francisella tularensis subsp. tularensis FSC198 universal stress protein YP_666430.1 258388 D 393115 CDS YP_666431.1 110669874 4199621 join(259584..259910,259910..260368) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 260368 isftu1 4199621 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666431.1 259584 D 393115 CDS YP_666432.1 110669875 4200357 260538..260909 1 NC_008245.1 ORF ftt0248; hypothetical protein 260909 4200357 FTF0248 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666432.1 260538 D 393115 CDS YP_666433.1 110669876 4199409 261010..263253 1 NC_008245.1 Similar to Q8ZJH5 Ferrous iron transport protein B from Yersinia pestis (771 aa). FASTA: opt: 1928 Z-score: 2034.7 E(): 1.9e-105 Smith-Waterman score: 2307; 45.396 identity in 771 aa overlap; ferrous iron transport protein 263253 feoB 4199409 feoB Francisella tularensis subsp. tularensis FSC198 ferrous iron transport protein YP_666433.1 261010 D 393115 CDS YP_666434.1 110669877 4199933 264065..266713 1 NC_008245.1 catalyzes the formation of phosphoenolpyruvate from pyruvate; pyruvate phosphate dikinase 266713 ppdK 4199933 ppdK Francisella tularensis subsp. tularensis FSC198 pyruvate phosphate dikinase YP_666434.1 264065 D 393115 CDS YP_666435.1 110669878 4200665 267113..268000 1 NC_008245.1 Similar to Q8Y1X0 Probable branched-chain amino acid aminotransferase protein from Ralstonia solanacearum (309 aa). FASTA: opt: 706 Z-score: 903.1 E(): 2.1e-42 Smith-Waterman score: 706; 39.931 identity in 288 aa overlap; branched-chain amino acid aminotransferase 268000 ilvE 4200665 ilvE Francisella tularensis subsp. tularensis FSC198 branched-chain amino acid aminotransferase YP_666435.1 267113 D 393115 CDS YP_666436.1 110669879 4199513 268011..269384 1 NC_008245.1 Similar to Q9C5X5 2-isopropylmalate synthase from Arabidopsis thaliana (503 aa). FASTA: opt: 493 Z-score: 601.2 E(): 1.4e-25 Smith-Waterman score: 635; 31.373 identity in 408 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; 2-isopropylmalate synthase 269384 leuA 4199513 leuA Francisella tularensis subsp. tularensis FSC198 2-isopropylmalate synthase YP_666436.1 268011 D 393115 CDS YP_666437.1 110669880 4199622 complement(join(269481..269939,269939..270265)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 270265 isftu1 4199622 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666437.1 269481 R 393115 CDS YP_666438.1 110669881 4200358 complement(270408..270779) 1 NC_008245.1 ORF ftt0254c; hypothetical protein 270779 4200358 FTF0254c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666438.1 270408 R 393115 CDS YP_666439.1 110669882 4200359 complement(270772..271713) 1 NC_008245.1 ORF ftt0255c; hypothetical protein 271713 4200359 FTF0255c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666439.1 270772 R 393115 CDS YP_666440.1 110669883 4200360 complement(272050..273018) 1 NC_008245.1 Similar to Q87T79 Putative lipopolysaccharide A protein from Vibrio parahaemolyticus (311 aa). FASTA: opt: 835 Z-score: 996.3 E(): 1.3e-47 Smith-Waterman score: 910; 43.671 identity in 316 aa overlap ORF ftt0256c; lipopolysaccharide protein 273018 4200360 FTF0256c Francisella tularensis subsp. tularensis FSC198 lipopolysaccharide protein YP_666440.1 272050 R 393115 CDS YP_666441.1 110669884 4200361 273207..275063 1 NC_008245.1 Similar to Q8YNW1 Ribonuclease II family protein from Anabaena sp. (686 aa). FASTA: opt: 557 Z-score: 649.1 E(): 2.9e-28 Smith-Waterman score: 763; 28.290 identity in 661 aa overlap ORF ftt0257; ribonuclease II family protein 275063 4200361 FTF0257 Francisella tularensis subsp. tularensis FSC198 ribonuclease II family protein YP_666441.1 273207 D 393115 CDS YP_666442.1 110669885 4200362 275394..276062 1 NC_008245.1 Similar to Q83AC9 Carboxylesterase/phospholipase family protein from Coxiella burnetii (200 aa). FASTA: opt: 599 Z-score: 736.0 E(): 4.2e-33 Smith-Waterman score: 599; 45.813 identity in 203 aa overlap ORF ftt0258; carboxylesterase/phospholipase family protein 276062 4200362 FTF0258 Francisella tularensis subsp. tularensis FSC198 carboxylesterase/phospholipase family protein YP_666442.1 275394 D 393115 CDS YP_666443.1 110669886 4200372 276059..276961 1 NC_008245.1 transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis; porphobilinogen deaminase 276961 hemC 4200372 hemC Francisella tularensis subsp. tularensis FSC198 porphobilinogen deaminase YP_666443.1 276059 D 393115 CDS YP_666444.1 110669887 4200275 276962..277354 1 NC_008245.1 may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling; camphor resistance protein CrcB 277354 crcB 4200275 crcB Francisella tularensis subsp. tularensis FSC198 camphor resistance protein CrcB YP_666444.1 276962 D 393115 CDS YP_666445.1 110669888 4200401 277351..277758 1 NC_008245.1 The predicted signal peptide are in conflict with the membrane lipoprotein lipid attachment site ORF ftt0261; hypothetical protein 277758 4200401 FTF0261 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666445.1 277351 D 393115 CDS YP_666446.1 110669889 4200402 277898..278035 1 NC_008245.1 The predicted signal peptide and the membrane lipoprotein lipid attachment site are in conflict with each other ORF ftt0262; lipoprotein 278035 4200402 FTF0262 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666446.1 277898 D 393115 CDS YP_666447.1 110669890 4200403 278088..278468 1 NC_008245.1 ORF ftt0263; hypothetical protein 278468 4200403 FTF0263 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666447.1 278088 D 393115 CDS YP_666448.1 110669891 4200404 complement(278465..278638) 1 NC_008245.1 Similar to Q8Y1X1 Hypothetical protein RSc0566 from Ralstonia solancearum (65 aa). FASTA: opt: 160 Z-score: 236.6 E(): 2.5e-05 Smith-Waterman score: 160; 50.000 identity in 36 aa overlap ORF ftt0264c; hypothetical protein 278638 4200404 FTF0264c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666448.1 278465 R 393115 CDS YP_666449.1 110669892 4200405 278767..280566 1 NC_008245.1 Similar to Q83AV7 ABC transporter,permease protein from Coxiella burnetii (581 aa). FASTA: opt: 976 Z-score: 1115.9 E(): 2.9e-54 Smith-Waterman score: 1738; 45.318 identity in 598 aa overlap ORF ftt0265; ABC transporter membrane protein 280566 4200405 FTF0265 Francisella tularensis subsp. tularensis FSC198 ABC transporter membrane protein YP_666449.1 278767 D 393115 CDS YP_666450.1 110669893 4200406 280585..281901 1 NC_008245.1 Similar to Q83AV8 ABC transporter, ATP-binding protein from Coxiella burnetii (432 aa). FASTA: opt: 1556 Z-score: 1713.5 E(): 1.5e-87 Smith-Waterman score: 1556; 54.673 identity in 428 aa overlap ORF ftt0266; ABC transporter ATP-binding protein 281901 4200406 FTF0266 Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_666450.1 280585 D 393115 CDS YP_666451.1 110669894 4200407 282026..283144 1 NC_008245.1 ORF ftt0267; hypothetical protein 283144 4200407 FTF0267 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666451.1 282026 D 393115 CDS YP_666452.1 110669895 4200408 283238..285124 1 NC_008245.1 Similar to Q88MV1 Sodium/hydrogen exchanger family protein from Pseudomonas putida (601 aa). FASTA: opt: 1318 Z-score: 1419.4 E(): 3.6e-71 Smith-Waterman score: 1318; 37.993 identity in 608 aa overlap ORF ftt0268; Sodium/hydrogen exchanger (antiporter) family protein 285124 4200408 FTF0268 Francisella tularensis subsp. tularensis FSC198 Sodium/hydrogen exchanger (antiporter) family protein YP_666452.1 283238 D 393115 CDS YP_666453.1 110669896 4200409 285109..285648 1 NC_008245.1 Similar to Q8EKP8 Carbonic anhydrase,family 3 from Shewanella oneidensis (182 aa). FASTA: opt: 639 Z-score: 840.8 E(): 6.1e-39 Smith-Waterman score: 639; 50.000 identity in 178 aa overlap ORF ftt0269; carbonic anhydrase 285648 4200409 FTF0269 Francisella tularensis subsp. tularensis FSC198 carbonic anhydrase YP_666453.1 285109 D 393115 CDS YP_666454.1 110669897 4199559 285599..286234 1 NC_008245.1 Similar to Q83AQ2 Outer membrane lipoprotein LolB,putative from Coxiella burnetii (210 aa). FASTA: opt: 395 Z-score: 508.7 E(): 1.9e-20 Smith-Waterman score: 395; 33.668 identity in 199 aa overlap; lipoprotein releasing system, subunit B, outer membrane lipoprotein 286234 lolB 4199559 lolB Francisella tularensis subsp. tularensis FSC198 lipoprotein releasing system, subunit B, outer membrane lipoprotein YP_666454.1 285599 D 393115 CDS YP_666455.1 110669898 4199301 286222..287049 1 NC_008245.1 An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis; 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 287049 ipk 4199301 ipk Francisella tularensis subsp. tularensis FSC198 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase YP_666455.1 286222 D 393115 CDS YP_666456.1 110669899 4200410 287238..287558 1 NC_008245.1 ORF ftt0272; hypothetical protein 287558 4200410 FTF0272 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666456.1 287238 D 393115 CDS YP_666457.1 110669900 4199623 join(287582..287908,287908..288366) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 288366 isftu1 4199623 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666457.1 287582 D 393115 CDS YP_666458.1 110669901 4200411 288480..288698 1 NC_008245.1 ORF ftt0274; hypothetical protein 288698 4200411 FTF0274 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666458.1 288480 D 393115 CDS YP_666459.1 110669902 4200414 complement(290443..290541) 1 NC_008245.1 Similar to Q937K9 YbgT protein from Erwinia chrysanthemi (38 aa). FASTA: opt: 81 Z-score: 153.9 E(): 1 Smith-Waterman score: 81; 42.857 identity in 28 aa overlap ORF ftt0277c; hypothetical protein 290541 4200414 FTF0277c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666459.1 290443 R 393115 CDS YP_666460.1 110669903 4200283 complement(290557..291750) 1 NC_008245.1 Similar to AAO69749 (Q8XGM0) Cytochrome d ubiquinol oxidase subunit II from Salmonella typhi (379 aa). FASTA: opt: 645 Z-score: 711.2 E(): 1e-31 Smith-Waterman score: 1022; 40.506identity in 395 aa overlap; cytochrome d terminal oxidase, polypeptide subunit II 291750 cydB 4200283 cydB Francisella tularensis subsp. tularensis FSC198 cytochrome d terminal oxidase, polypeptide subunit II YP_666460.1 290557 R 393115 CDS YP_666461.1 110669904 4200282 complement(291761..293539) 1 NC_008245.1 Similar to AAP96534 (Q7VKT6) Cytochrome D ubiquinol oxidase, subunit I from Haemophilus ducreyi (516 aa). FASTA: opt: 1311 Z-score: 1457.7 E(): 2.7e-73 Smith-Waterman score: 1688; 47.478identity in 575 aa overlap Schu S4 seems to have extra sequence in between 340-420 aa; cytochrome d terminal oxidase, polypeptide subunit I 293539 cydA 4200282 cydA Francisella tularensis subsp. tularensis FSC198 cytochrome d terminal oxidase, polypeptide subunit I YP_666461.1 291761 R 393115 CDS YP_666462.1 110669905 4200700 complement(293968..295335) 1 NC_008245.1 Similar to AAO89830 (Q83EP3) Major facilitator family transporter from Coxiella burnetii (458 aa). FASTA: opt: 1379 Z-score: 1410.6 E(): 1.1e-70 Smith-Waterman score: 1379; 46.102 identity in 449 aa overlap; major facilitator transporter 295335 yajR 4200700 yajR Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666462.1 293968 R 393115 CDS YP_666463.1 110669906 4199516 295696..296598 1 NC_008245.1 Similar to Q87DQ7 Cytochrome O ubiquinol oxidase subunit II from Xylella fastidiosa (319 aa). FASTA: opt: 956 Z-score: 1145.6 E(): 6.4e-56 Smith-Waterman score: 956; 49.477 identity in 287 aa overlap; cytochrome O ubiquinol oxidase subunit II 296598 cyoA 4199516 cyoA Francisella tularensis subsp. tularensis FSC198 cytochrome O ubiquinol oxidase subunit II YP_666463.1 295696 D 393115 CDS YP_666464.1 110669907 4199517 296637..298667 1 NC_008245.1 Similar to AAO70008 (Q8XG02) Cytochrome o ubiquinol oxidase subunit I from Salmonella typhi (663 aa). FASTA: opt: 3045 Z-score: 3221.2 E(): 1.6e-171 Smith-Waterman score: 3045; 64.361identity in 665 aa overlap; cytochrome O ubiquinol oxidase subunit I 298667 cyoB 4199517 cyoB Francisella tularensis subsp. tularensis FSC198 cytochrome O ubiquinol oxidase subunit I YP_666464.1 296637 D 393115 CDS YP_666465.1 110669908 4199518 298664..299266 1 NC_008245.1 Similar to Q87IH7 Cytochrome o ubiquinol oxidase,subunit III from Vibrio parahaemolyticus (200 aa). FASTA: opt: 768 Z-score: 931.9 E(): 5.1e-44 Smith-Waterman score: 768; 55.276identity in 199 aa overlap; cytochrome O ubiquinol oxidase, subunit III 299266 cyoC 4199518 cyoC Francisella tularensis subsp. tularensis FSC198 cytochrome O ubiquinol oxidase, subunit III YP_666465.1 298664 D 393115 CDS YP_666466.1 110669909 4199519 299268..299600 1 NC_008245.1 Similar to Q8ZC55 Cytochrome O ubiquinol oxidase subunit IV from Yersinia pestis (110 aa). FASTA: opt: 325 Z-score: 419.7 E(): 1.7e-15 Smith-Waterman score: 325; 50.505 identity in 99 aa overlap; cytochrome O ubiquinol oxidase subunit IV 299600 cyoD 4199519 cyoD Francisella tularensis subsp. tularensis FSC198 cytochrome O ubiquinol oxidase subunit IV YP_666466.1 299268 D 393115 CDS YP_666467.1 110669910 4199520 299631..300479 1 NC_008245.1 converts protoheme IX and farnesyl diphosphate to heme O; protoheme IX farnesyltransferase 300479 cyoE 4199520 cyoE Francisella tularensis subsp. tularensis FSC198 protoheme IX farnesyltransferase YP_666467.1 299631 D 393115 CDS YP_666468.1 110669911 4199648 complement(300624..301667) 1 NC_008245.1 Similar to Q8A014 UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase from Bacteroides thetaiotaomicron (346 aa). FASTA: opt: 687 Z-score: 795.8 E(): 2e-36 Smith-Waterman score: 687; 33.431 identity in 341 aa overlap paralog of FTF1571; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase 301667 lpxD2 4199648 lpxD2 Francisella tularensis subsp. tularensis FSC198 UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase YP_666468.1 300624 R 393115 CDS YP_666469.1 110669912 4200415 complement(301735..302430) 1 NC_008245.1 Similar to Q8XIW2 Hypothetical protein CPE2001 from Clostridium perfringens (229 aa). FASTA: opt: 806 Z-score: 994.2 E(): 1.7e-47 Smith-Waterman score: 806; 51.948 identity in 231 aa overlap ORF ftt0287c; hypothetical protein 302430 4200415 FTF0287c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666469.1 301735 R 393115 CDS YP_666470.1 110669913 4199238 complement(302430..303281) 1 NC_008245.1 Similar to Q8D4Q2 Pyridoxal/pyridoxine/pyridoxamine kinase from Vibrio vulnificus (290 aa). FASTA: opt: 611 Z-score: 766.0 E(): 8.9e-35 Smith-Waterman score: 611; 38.545identity in 275 aa overlap; pyridoxal kinase 303281 pdxY 4199238 pdxY Francisella tularensis subsp. tularensis FSC198 pyridoxal kinase YP_666470.1 302430 R 393115 CDS YP_666471.1 110669914 4200416 complement(303288..303722) 1 NC_008245.1 ORF ftt0289c; hypothetical protein 303722 4200416 FTF0289c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666471.1 303288 R 393115 CDS YP_666472.1 110669915 4199850 303868..304824 1 NC_008245.1 Similar to Q87YN6 MoxR protein, putative, from Pseudomonas syringae (319 aa). FASTA: opt: 1309 Z-score: 1518.5 E(): 9.9e-77 Smith-Waterman score: 1309; 61.489 identity in 309 aa overlap; methanol dehydrogenase regulatory protein 304824 moxR 4199850 moxR Francisella tularensis subsp. tularensis FSC198 methanol dehydrogenase regulatory protein YP_666472.1 303868 D 393115 CDS YP_666473.1 110669916 4200417 304837..305748 1 NC_008245.1 Similar to Q87YN5 Conserved hypothetical protein from Pseudomonas syringae (314 aa). FASTA: opt: 561 Z-score: 693.3 bits: 136.4 E(): 1e-30 Smith-Waterman score: 561; 30.479 identity in 292 aa overlap ORF ftt0291; hypothetical protein 305748 4200417 FTF0291 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666473.1 304837 D 393115 CDS YP_666474.1 110669917 4200418 305738..306214 1 NC_008245.1 Similar to Q8D3W9 Conserved hypothetical protein from Vibrio vulnificus (156 aa). FASTA: opt: 228 Z-score: 276.8 E(): 1.5e-07 Smith-Waterman score: 228; 28.481 identity in 158 aa overlap ORF ftt0292; hypothetical protein 306214 4200418 FTF0292 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666474.1 305738 D 393115 CDS YP_666475.1 110669918 4200419 306193..307212 1 NC_008245.1 Similar to Q88LA6 Von Willebrand factor type A domain protein from Pseuodmonas putida (358 aa). FASTA: opt: 905 Z-score: 1015.4 E(): 1.1e-48 Smith-Waterman score: 905; 45.122 identity in 328 aa overlap ORF ftt0293; hypothetical protein 307212 4200419 FTF0293 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666475.1 306193 D 393115 CDS YP_666476.1 110669919 4200421 308945..310570 1 NC_008245.1 Similar to Q8ECP0 Conserved hypothetical protein from Shewanella oneidensis (555 aa). FASTA: opt: 529 Z-score: 628.4 E(): 4.1e-27 Smith-Waterman score: 529; 24.371 identity in 517 aa overlap ORF ftt0295; hypothetical protein 310570 4200421 FTF0295 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666476.1 308945 D 393115 CDS YP_666477.1 110669920 4199228 310571..311248 1 NC_008245.1 Similar to PCP_CLOPE (Q8XKH1) Pyrrolidone-carboxylate peptidase from Clostridium perfringens (215 aa). FASTA: opt: 710 Z-score: 844.3 E(): 3.9e-39 Smith-Waterman score: 710; 52.020identity in 198 aa overlap; pyrrolidone-carboxylate peptidase 311248 pcp 4199228 pcp Francisella tularensis subsp. tularensis FSC198 pyrrolidone-carboxylate peptidase YP_666477.1 310571 D 393115 CDS YP_666478.1 110669921 4200422 311256..311738 1 NC_008245.1 ORF ftt0297; putative cytochrome c-type biogenesis protein 311738 4200422 FTF0297 Francisella tularensis subsp. tularensis FSC198 putative cytochrome c-type biogenesis protein YP_666478.1 311256 D 393115 CDS YP_666479.1 110669922 4200199 311769..312209 1 NC_008245.1 Similar to Q8XWQ9 Probable DNA polymerase III (CHI subunit) protein from Ralstonia solanacearum (142 aa). FASTA: opt: 142 Z-score: 187.9 E(): 0.013 Smith-Waterman score: 142; 30.000identity in 120 aa overlap; DNA polymerase III subunit chi 312209 holC 4200199 holC Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit chi YP_666479.1 311769 D 393115 CDS YP_666480.1 110669923 4200138 312341..315100 1 NC_008245.1 valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain; valyl-tRNA synthetase 315100 valS 4200138 valS Francisella tularensis subsp. tularensis FSC198 valyl-tRNA synthetase YP_666480.1 312341 D 393115 CDS YP_666481.1 110669924 4200423 315129..315983 1 NC_008245.1 ORF ftt0300; hypothetical protein 315983 4200423 FTF0300 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666481.1 315129 D 393115 CDS YP_666482.1 110669925 4200424 316056..316925 1 NC_008245.1 ORF ftt0301; hypothetical protein 316925 4200424 FTF0301 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666482.1 316056 D 393115 CDS YP_666483.1 110669926 4200425 317109..317444 1 NC_008245.1 ORF ftt0302; hypothetical protein 317444 4200425 FTF0302 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666483.1 317109 D 393115 CDS YP_666484.1 110669927 4199556 complement(317441..318598) 1 NC_008245.1 Similar to Q9JTX1 L-lactate dehydrogenase from Neisseria meningitidis (390 aa). FASTA: opt: 1591 Z-score: 1977.5 E(): 3e-102 Smith-Waterman score: 1591; 60.789 identity in 380 aa overlap; L-lactate dehydrogenase 318598 lldD1 4199556 lldD1 Francisella tularensis subsp. tularensis FSC198 L-lactate dehydrogenase YP_666484.1 317441 R 393115 CDS YP_666485.1 110669928 4200239 complement(318625..319278) 1 NC_008245.1 catalyzes the reduction of nitroaromatic compounds such as nitrofurazone, quinones and the anti-tumor agent CB1954; NAD(P)H-dependent; oxygen insensitive; dihydropteridine reductase 319278 nfnB 4200239 nfnB Francisella tularensis subsp. tularensis FSC198 dihydropteridine reductase YP_666485.1 318625 R 393115 CDS YP_666486.1 110669929 4200426 319418..319795 1 NC_008245.1 Similar to Q88NZ1 MutT/nudix family protein from Pseuodomonas putida (137 aa). FASTA: opt: 224 Z-score: 293.2 E(): 1.8e-08 Smith-Waterman score: 224; 34.545 identity in 110 aa overlap ORF ftt0305; MutT/nudix family protein 319795 4200426 FTF0305 Francisella tularensis subsp. tularensis FSC198 MutT/nudix family protein YP_666486.1 319418 D 393115 CDS YP_666487.1 110669930 4199201 319968..321359 1 NC_008245.1 class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle; fumarate hydratase 321359 fumC 4199201 fumC Francisella tularensis subsp. tularensis FSC198 fumarate hydratase YP_666487.1 319968 D 393115 CDS YP_666488.1 110669931 4199800 321489..322895 1 NC_008245.1 Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation; glutamyl-tRNA synthetase 322895 gltX 4199800 gltX Francisella tularensis subsp. tularensis FSC198 glutamyl-tRNA synthetase YP_666488.1 321489 D 393115 CDS YP_666489.1 110669932 4200427 322886..323863 1 NC_008245.1 ORF ftt0308; hypothetical protein 323863 4200427 FTF0308 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666489.1 322886 D 393115 CDS YP_666490.1 110669933 4200191 complement(323887..324630) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 324630 isftu2 4200191 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666490.1 323887 R 393115 CDS YP_666491.1 110669934 4200428 324926..326380 1 NC_008245.1 Similar to Q8XT33 Probable amino-acid permease transmembrane protein from Ralstonia solanacearum (543 aa). FASTA: opt: 999 Z-score: 1146.0 E(): 5.5e-5 Smith-Waterman score: 999; 34.924 identity in 461 aa overlap ORF ftt0310; amino acid permease 326380 4200428 FTF0310 Francisella tularensis subsp. tularensis FSC198 amino acid permease YP_666491.1 324926 D 393115 CDS YP_666492.1 110669935 4200429 complement(326712..327635) 1 NC_008245.1 Similar to Q9KKV6 Hypothetical protein VCA0994 from Vibrio cholerae (326 aa). FASTA: opt: 558 Z-score: 686.8 E(): 2.3e-30 Smith-Waterman score: 558; 31.949 identity in 313 aa overlap ORF ftt0311c; hypothetical protein 327635 4200429 FTF0311c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666492.1 326712 R 393115 CDS YP_666493.1 110669936 4199413 complement(327717..328217) 1 NC_008245.1 Similar to Q9K7B6 Dihydrofolate reductase from Bacillus halodurans (163 aa). FASTA: opt: 465 Z-score: 598.3 E(): 2e-25 Smith-Waterman score: 465; 41.566 identity in 166 aa overlap; dihydrofolate reductase type I 328217 folA 4199413 folA Francisella tularensis subsp. tularensis FSC198 dihydrofolate reductase type I YP_666493.1 327717 R 393115 CDS YP_666494.1 110669937 4200334 328449..329168 1 NC_008245.1 one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit; 30S ribosomal protein S2 329168 rpsB 4200334 rpsB Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S2 YP_666494.1 328449 D 393115 CDS YP_666495.1 110669938 4198972 329190..330059 1 NC_008245.1 EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu; elongation factor Ts 330059 tsf 4198972 tsf Francisella tularensis subsp. tularensis FSC198 elongation factor Ts YP_666495.1 329190 D 393115 CDS YP_666496.1 110669939 4200592 330063..330812 1 NC_008245.1 Catalyzes the phosphorylation of UMP to UDP; uridylate kinase 330812 pyrH 4200592 pyrH Francisella tularensis subsp. tularensis FSC198 uridylate kinase YP_666496.1 330063 D 393115 CDS YP_666497.1 110669940 4199190 330841..331398 1 NC_008245.1 Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs; ribosome recycling factor 331398 frr 4199190 frr Francisella tularensis subsp. tularensis FSC198 ribosome recycling factor YP_666497.1 330841 D 393115 CDS YP_666498.1 110669941 4198985 331431..332204 1 NC_008245.1 catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate; undecaprenyl pyrophosphate synthase 332204 uppS 4198985 uppS Francisella tularensis subsp. tularensis FSC198 undecaprenyl pyrophosphate synthase YP_666498.1 331431 D 393115 CDS YP_666499.1 110669942 4200320 332218..333012 1 NC_008245.1 Similar to Q88MH5 Phosphatidate cytidylyltransferase from Pseudomonas putida (271 aa). FASTA: 398 opt: 534 Z-score: 626.6 E(): 5.2e-27 Smith-Waterman score: 536; 35.926 identity in 270 aa overlap; phosphatidate cytidylyltransferase 333012 cdsA 4200320 cdsA Francisella tularensis subsp. tularensis FSC198 phosphatidate cytidylyltransferase YP_666499.1 332218 D 393115 CDS YP_666500.1 110669943 4199666 333022..333468 1 NC_008245.1 catalyzes the formation of dUMP from dUTP; deoxyuridine 5'-triphosphate nucleotidohydrolase 333468 dut 4199666 dut Francisella tularensis subsp. tularensis FSC198 deoxyuridine 5'-triphosphate nucleotidohydrolase YP_666500.1 333022 D 393115 CDS YP_666501.1 110669944 4199245 333472..334068 1 NC_008245.1 Similar to Q8XFD0 Phosphotidylglycerophosphate synthetase from Salmonella typhi (182 aa). FASTA: opt: 575 Z-score: 728.4 E(): 1.1e-32 Smith-Waterman score: 618; 50.000identity in 188 aa overlap; phosphatidylglycerophosphate synthetase 334068 pgsA 4199245 pgsA Francisella tularensis subsp. tularensis FSC198 phosphatidylglycerophosphate synthetase YP_666501.1 333472 D 393115 CDS YP_666502.1 110669945 4198950 334203..334577 1 NC_008245.1 interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance; 30S ribosomal protein S12 334577 rpsL 4198950 rpsL Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S12 YP_666502.1 334203 D 393115 CDS YP_666503.1 110669946 4200339 334616..335089 1 NC_008245.1 binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit; 30S ribosomal protein S7 335089 rpsG 4200339 rpsG Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S7 YP_666503.1 334616 D 393115 CDS YP_666504.1 110669947 4199203 335104..337218 1 NC_008245.1 EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene; elongation factor G 337218 fusA 4199203 fusA Francisella tularensis subsp. tularensis FSC198 elongation factor G YP_666504.1 335104 D 393115 CDS YP_666505.1 110669948 4200342 337239..337556 1 NC_008245.1 NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex; 30S ribosomal protein S10 337556 rpsJ 4200342 rpsJ Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S10 YP_666505.1 337239 D 393115 CDS YP_666506.1 110669949 4200775 337660..338295 1 NC_008245.1 binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin; 50S ribosomal protein L3 338295 rplC 4200775 rplC Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L3 YP_666506.1 337660 D 393115 CDS YP_666507.1 110669950 4200776 338325..338948 1 NC_008245.1 L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA; 50S ribosomal protein L4 338948 rplD 4200776 rplD Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L4 YP_666507.1 338325 D 393115 CDS YP_666508.1 110669951 4198999 338945..339244 1 NC_008245.1 binds third domain of 23S rRNA and protein L29; part of exit tunnel; 50S ribosomal protein L23 339244 rplW 4198999 rplW Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L23 YP_666508.1 338945 D 393115 CDS YP_666509.1 110669952 4200774 339266..340090 1 NC_008245.1 one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; 50S ribosomal protein L2 340090 rplB 4200774 rplB Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L2 YP_666509.1 339266 D 393115 CDS YP_666510.1 110669953 4198957 340105..340383 1 NC_008245.1 protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA; 30S ribosomal protein S19 340383 rpsS 4198957 rpsS Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S19 YP_666510.1 340105 D 393115 CDS YP_666511.1 110669954 4198998 340399..340734 1 NC_008245.1 binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center; 50S ribosomal protein L22 340734 rplV 4198998 rplV Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L22 YP_666511.1 340399 D 393115 CDS YP_666512.1 110669955 4200335 340749..341417 1 NC_008245.1 forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation; 30S ribosomal protein S3 341417 rpsC 4200335 rpsC Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S3 YP_666512.1 340749 D 393115 CDS YP_666513.1 110669956 4198992 341421..341834 1 NC_008245.1 located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e; 50S ribosomal protein L16 341834 rplP 4198992 rplP Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L16 YP_666513.1 341421 D 393115 CDS YP_666514.1 110669957 4199004 341834..342034 1 NC_008245.1 one of the stabilizing components for the large ribosomal subunit; 50S ribosomal protein L29 342034 rpmC 4199004 rpmC Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L29 YP_666514.1 341834 D 393115 CDS YP_666515.1 110669958 4198955 342047..342298 1 NC_008245.1 primary binding protein; helps mediate assembly; involved in translation fidelity; 30S ribosomal protein S17 342298 rpsQ 4198955 rpsQ Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S17 YP_666515.1 342047 D 393115 CDS YP_666516.1 110669959 4198990 342390..342758 1 NC_008245.1 binds to the 23S rRNA between the centers for peptidyl transferase and GTPase; 50S ribosomal protein L14 342758 rplN 4198990 rplN Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L14 YP_666516.1 342390 D 393115 CDS YP_666517.1 110669960 4199000 342780..343097 1 NC_008245.1 assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel; 50S ribosomal protein L24 343097 rplX 4199000 rplX Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L24 YP_666517.1 342780 D 393115 CDS YP_666518.1 110669961 4200777 343109..343648 1 NC_008245.1 part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13; 50S ribosomal protein L5 343648 rplE 4200777 rplE Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L5 YP_666518.1 343109 D 393115 CDS YP_666519.1 110669962 4198952 343667..343972 1 NC_008245.1 located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif; 30S ribosomal protein S14 343972 rpsN 4198952 rpsN Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S14 YP_666519.1 343667 D 393115 CDS YP_666520.1 110669963 4200340 343989..344387 1 NC_008245.1 binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit; 30S ribosomal protein S8 344387 rpsH 4200340 rpsH Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S8 YP_666520.1 343989 D 393115 CDS YP_666521.1 110669964 4200778 344406..344942 1 NC_008245.1 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance; 50S ribosomal protein L6 344942 rplF 4200778 rplF Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L6 YP_666521.1 344406 D 393115 CDS YP_666522.1 110669965 4198994 344965..345318 1 NC_008245.1 binds 5S rRNA along with protein L5 and L25; 50S ribosomal protein L18 345318 rplR 4198994 rplR Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L18 YP_666522.1 344965 D 393115 CDS YP_666523.1 110669966 4200337 345346..345846 1 NC_008245.1 located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance; 30S ribosomal protein S5 345846 rpsE 4200337 rpsE Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S5 YP_666523.1 345346 D 393115 CDS YP_666524.1 110669967 4199005 345853..346038 1 NC_008245.1 L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7; 50S ribosomal protein L30 346038 rpmD 4199005 rpmD Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L30 YP_666524.1 345853 D 393115 CDS YP_666525.1 110669968 4198991 346045..346476 1 NC_008245.1 late assembly protein; 50S ribosomal protein L15 346476 rplO 4198991 rplO Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L15 YP_666525.1 346045 D 393115 CDS YP_666526.1 110669969 4200740 346487..347812 1 NC_008245.1 forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase; preprotein translocase subunit SecY 347812 secY 4200740 secY Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecY YP_666526.1 346487 D 393115 CDS YP_666527.1 110669970 4200325 347836..347949 1 NC_008245.1 smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif; 50S ribosomal protein L36 347949 rpmJ 4200325 rpmJ Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L36 YP_666527.1 347836 D 393115 CDS YP_666528.1 110669971 4198951 348066..348422 1 NC_008245.1 located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA; 30S ribosomal protein S13 348422 rpsM 4198951 rpsM Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S13 YP_666528.1 348066 D 393115 CDS YP_666529.1 110669972 4198949 348462..348851 1 NC_008245.1 located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3; 30S ribosomal protein S11 348851 rpsK 4198949 rpsK Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S11 YP_666529.1 348462 D 393115 CDS YP_666530.1 110669973 4200336 348873..349493 1 NC_008245.1 primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination; 30S ribosomal protein S4 349493 rpsD 4200336 rpsD Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S4 YP_666530.1 348873 D 393115 CDS YP_666531.1 110669974 4200326 349551..350522 1 NC_008245.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 350522 rpoA1 4200326 rpoA1 Francisella tularensis subsp. tularensis FSC198 DNA-directed RNA polymerase subunit alpha YP_666531.1 349551 D 393115 CDS YP_666532.1 110669975 4198993 350567..351004 1 NC_008245.1 is a component of the macrolide binding site in the peptidyl transferase center; 50S ribosomal protein L17 351004 rplQ 4198993 rplQ Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L17 YP_666532.1 350567 D 393115 CDS YP_666533.1 110669976 4199624 join(351158..351484,351484..351942) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 351942 isftu1 4199624 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666533.1 351158 D 393115 CDS YP_666534.1 110669977 4200430 352871..353866 1 NC_008245.1 Duplicate of FTF0378 and FTF1263 ORF ftt0354; hypothetical protein 353866 4200430 FTF0354 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666534.1 352871 D 393115 CDS YP_666535.1 110669978 4199625 complement(join(354158..354616,354616..354942)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 354942 isftu1 4199625 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666535.1 354158 R 393115 CDS YP_666536.1 110669979 4200210 355104..356990 1 NC_008245.1 molecular chaperone; heat shock protein 90 356990 htpG 4200210 htpG Francisella tularensis subsp. tularensis FSC198 heat shock protein 90 YP_666536.1 355104 D 393115 CDS YP_666537.1 110669980 4199626 complement(join(357314..357772,357772..358098)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 358098 isftu1 4199626 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666537.1 357314 R 393115 CDS YP_666538.1 110669981 4200432 359366..359836 1 NC_008245.1 ORF ftt0359; hypothetical protein 359836 4200432 FTF0359 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666538.1 359366 D 393115 CDS YP_666539.1 110669982 4200433 359973..360797 1 NC_008245.1 Similar to Q82V84 Short-chain dehydrogenase/reductase (SDR) superfamily from Nitrosomonas europaea (279 aa). FASTA: opt: 829 Z-score: 982.8 E(): 7.5e-47 Smith-Waterman score: 829; 49.632 identity in 272 aa overlap ORF ftt0360; short-chain dehydrogenase 360797 4200433 FTF0360 Francisella tularensis subsp. tularensis FSC198 short-chain dehydrogenase YP_666539.1 359973 D 393115 CDS YP_666540.1 110669983 4200434 complement(360819..362168) 1 NC_008245.1 Similar to Q8Z506 Putative amino acid transporter from Salmonella typhi (473 aa). FASTA: opt: 717 Z-score: 754.8 E(): 3.8e-34 Smith-Waterman score: 755; 29.783 identity in 460 aa overlap ORF ftt0361c; amino acid transporter 362168 4200434 FTF0361c Francisella tularensis subsp. tularensis FSC198 amino acid transporter YP_666540.1 360819 R 393115 CDS YP_666541.1 110669984 4200435 complement(362427..363299) 1 NC_008245.1 ORF ftt0362c; hypothetical protein 363299 4200435 FTF0362c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666541.1 362427 R 393115 CDS YP_666542.1 110669985 4199627 join(363467..363793,363793..364251) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 364251 isftu1 4199627 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666542.1 363467 D 393115 CDS YP_666543.1 110669986 4200436 complement(364375..364824) 1 NC_008245.1 ORF ftt0364c; hypothetical protein 364824 4200436 FTF0364c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666543.1 364375 R 393115 CDS YP_666544.1 110669987 4199742 365305..366036 1 NC_008245.1 Similar to Q8PFB8 Phenol hydroxylase from Xanthomonas axonopodis (365 aa). FASTA: opt: 567 Z-score: 717.5 E(): 4.5e-32 Smith-Waterman score: 567; 38.211 identity in 246 aa overlap The protein seems to be shorter (<115 aa) than the matching proteins.; phenol hydroxylase 366036 poxF 4199742 poxF Francisella tularensis subsp. tularensis FSC198 phenol hydroxylase YP_666544.1 365305 D 393115 CDS YP_666545.1 110669988 4199006 366158..366373 1 NC_008245.1 RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome; 50S ribosomal protein L31 366373 rpmE 4199006 rpmE Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L31 YP_666545.1 366158 D 393115 CDS YP_666546.1 110669989 4200304 complement(366584..368089) 1 NC_008245.1 Similar to Q88AR1 Glutamate--cysteine ligase from Pseudomonas syringae (529 aa). FASTA: opt: 1113 Z-score: 1281.1 E(): 1.8e-63 Smith-Waterman score: 1113; 39.250 identity in 507 aa overlap; glutamate--cysteine ligase 368089 gshA 4200304 gshA Francisella tularensis subsp. tularensis FSC198 glutamate--cysteine ligase YP_666546.1 366584 R 393115 CDS YP_666547.1 110669990 4200116 complement(368142..369686) 1 NC_008245.1 Similar to AAO89943 (Q83ED5) Integral membrane protein MviN from Coxiella burnetii (515 aa). FASTA: opt: 836 Z-score: 887.1 E(): 1.6e-41 Smith-Waterman score: 836; 30.452 identity in 509 aa overlap; virulence factor MviN 369686 mviN 4200116 mviN Francisella tularensis subsp. tularensis FSC198 virulence factor MviN YP_666547.1 368142 R 393115 CDS YP_666548.1 110669991 4200437 complement(369709..370770) 1 NC_008245.1 ORF ftt0369c; hypothetical protein 370770 4200437 FTF0369c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666548.1 369709 R 393115 CDS YP_666549.1 110669992 4199112 complement(370853..371263) 1 NC_008245.1 Similar to Q8Z189 Hypothetical protein yjeE from Salmonella typhi (153 aa). FASTA: opt: 517 Z-score: 623.3 E(): 7.9e-27 Smith-Waterman score: 517; 56.489 identity in 131 aa overlap; nucleotide-binding protein, yjeE 371263 yjeE 4199112 yjeE Francisella tularensis subsp. tularensis FSC198 nucleotide-binding protein, yjeE YP_666549.1 370853 R 393115 CDS YP_666550.1 110669993 4199415 complement(371241..372422) 1 NC_008245.1 Similar to Q8ECR5 FolC bifunctional protein from Shewanella oneidensis (431 aa). FASTA: opt: 507 Z-score: 630.2 E(): 3.3e-27 Smith-Waterman score: 627; 32.274 identity in 409 aa overlap; bifunctional folylpolyglutamate synthase/ dihydrofolate synthase 372422 folC 4199415 folC Francisella tularensis subsp. tularensis FSC198 bifunctional folylpolyglutamate synthase/ dihydrofolate synthase YP_666550.1 371241 R 393115 CDS YP_666551.1 110669994 4199749 complement(372437..373345) 1 NC_008245.1 Similar to Q8XFJ5 Acetyl-CoA carboxylase beta subunit from Salmonella typhi (304 aa). FASTA: opt: 1197 Z-score: 1430.2 E(): 9.1e-72 Smith-Waterman score: 1197; 62.116 identity in 293 aa overlap; Acetyl-CoA carboxylase beta subunit 373345 accD 4199749 accD Francisella tularensis subsp. tularensis FSC198 Acetyl-CoA carboxylase beta subunit YP_666551.1 372437 R 393115 CDS YP_666552.1 110669995 4200238 complement(373381..373803) 1 NC_008245.1 catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate; nucleoside diphosphate kinase 373803 ndk 4200238 ndk Francisella tularensis subsp. tularensis FSC198 nucleoside diphosphate kinase YP_666552.1 373381 R 393115 CDS YP_666553.1 110669996 4200591 complement(373876..375516) 1 NC_008245.1 CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer; CTP synthetase 375516 pyrG 4200591 pyrG Francisella tularensis subsp. tularensis FSC198 CTP synthetase YP_666553.1 373876 R 393115 CDS YP_666554.1 110669997 4200439 complement(377434..378492) 1 NC_008245.1 Weak hits at position aa 250-350 to many cytochrome oxidase subunit I eg:(Q85L50). ORF ftt0376c; hypothetical protein 378492 4200439 FTF0376c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666554.1 377434 R 393115 CDS YP_666555.1 110669998 4199628 join(378859..379188,379188..379643) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 379643 isftu1 4199628 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666555.1 378859 D 393115 CDS YP_666556.1 110669999 4200440 complement(379935..380930) 1 NC_008245.1 Duplicate of FTF0354 and FTF1263 ORF ftt0378c; hypothetical protein 380930 4200440 FTF0378c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666556.1 379935 R 393115 CDS YP_666557.1 110670000 4199282 complement(381967..383316) 1 NC_008245.1 converts 2-oxoglutarate to glutamate; in Escherichia coli this enzyme plays a role in glutamate synthesis when the cell is under energy restriction; uses NADPH; forms a homohexamer; glutamate dehydrogenase 383316 gdh 4199282 gdh Francisella tularensis subsp. tularensis FSC198 glutamate dehydrogenase YP_666557.1 381967 R 393115 CDS YP_666558.1 110670001 4199287 383655..384398 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 384398 isftu2 4199287 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666558.1 383655 D 393115 CDS YP_666559.1 110670002 4200441 384523..384627 1 NC_008245.1 ORF ftt0382; hypothetical protein 384627 4200441 FTF0382 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666559.1 384523 D 393115 CDS YP_666560.1 110670003 4200442 385638..385973 1 NC_008245.1 ORF ftt0383; hypothetical protein 385973 4200442 FTF0383 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666560.1 385638 D 393115 CDS YP_666561.1 110670004 4199946 complement(385946..386797) 1 NC_008245.1 catalyzes the decarboxylation of phosphatidyl-L-serine to phosphatidylethanoleamine; phosphatidylserine decarboxylase 386797 psd 4199946 psd Francisella tularensis subsp. tularensis FSC198 phosphatidylserine decarboxylase YP_666561.1 385946 R 393115 CDS YP_666562.1 110670005 4200443 386897..387877 1 NC_008245.1 ORF ftt0385; hypothetical protein 387877 4200443 FTF0385 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666562.1 386897 D 393115 CDS YP_666563.1 110670006 4200233 387886..388929 1 NC_008245.1 NadM-Nudix subfamily; involved in creation of nicotanimide adenine dinucleotide NAD from either biosynthetic or salvage pathways; bifunctional nicotinamide mononucleotide adenylyltransferase/ADP-ribose pyrophosphatase 388929 nadM 4200233 nadM Francisella tularensis subsp. tularensis FSC198 bifunctional nicotinamide mononucleotide adenylyltransferase/ADP-ribose pyrophosphatase YP_666563.1 387886 D 393115 CDS YP_666564.1 110670007 4199788 388943..390310 1 NC_008245.1 Similar to Q9KNH7 UDP-N-acetylglucosamine pyrophosphorylase from Vibrio cholerae (453 aa). FASTA: opt: 1597 Z-score: 1863.6 E(): 6.5e-96 Smith-Waterman score: 1597; 53.201 identity in 453 aa overlap; UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase 390310 glmU 4199788 glmU Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase YP_666564.1 388943 D 393115 CDS YP_666565.1 110670008 4199787 390331..392169 1 NC_008245.1 Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source; glucosamine--fructose-6-phosphate aminotransferase 392169 glmS 4199787 glmS Francisella tularensis subsp. tularensis FSC198 glucosamine--fructose-6-phosphate aminotransferase YP_666565.1 390331 D 393115 CDS YP_666566.1 110670009 4200444 392253..392777 1 NC_008245.1 Similar to Q9RWR2 Acetyltransferase, putative, from Deinococcus radiodurans (188 aa). FASTA: opt: 188 Z-score: 245.7 E(): 8.5e-06 Smith-Waterman score: 188; 28.105 identity in 153 aa overlap ORF ftt0389; acetyltransferase 392777 4200444 FTF0389 Francisella tularensis subsp. tularensis FSC198 acetyltransferase YP_666566.1 392253 D 393115 CDS YP_666567.1 110670010 4198959 complement(392770..392967) 1 NC_008245.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 392967 rpsU 4198959 rpsU Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S21 YP_666567.1 392770 R 393115 CDS YP_666568.1 110670011 4200280 complement(392967..393170) 1 NC_008245.1 Similar to Q8D6M5 Cold shock protein from Vibrio vulnificus (70 aa). FASTA: opt: 272 Z-score: 403.6 E(): 1.4e-14 Smith-Waterman score: 272; 58.571 identity in 70 aa overlap; cold shock protein 393170 cspC 4200280 cspC Francisella tularensis subsp. tularensis FSC198 cold shock protein YP_666568.1 392967 R 393115 CDS YP_666569.1 110670012 4200445 complement(393418..393747) 1 NC_008245.1 Similar to Q8TT97 Hypothetical protein MA0540 from Methanosarcina mazei (95 aa). FASTA: opt: 151 Z-score: 197.5 E(): 0.0038 Smith-Waterman score: 151; 35.789 identity in 95 aa overlap ORF ftt0392c; hypothetical protein 393747 4200445 FTF0392c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666569.1 393418 R 393115 CDS YP_666570.1 110670013 4199661 393874..394644 1 NC_008245.1 Similar to Q886P4 Methionine aminopeptidase,type I from Pseudomonas syringae (260 aa). FASTA: opt: 1243 Z-score: 1568.2 E(): 1.9e-79 Smith-Waterman score: 1243; 70.120 identity in 251 aa overlap; methionine aminopeptidase 394644 map 4199661 map Francisella tularensis subsp. tularensis FSC198 methionine aminopeptidase YP_666570.1 393874 D 393115 CDS YP_666571.1 110670014 4200446 394646..395494 1 NC_008245.1 ORF ftt0394; hypothetical protein 395494 4200446 FTF0394 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666571.1 394646 D 393115 CDS YP_666572.1 110670015 4200447 395540..396253 1 NC_008245.1 Similar to Q9L5J1 hypothetical protein from Salmonella typhi (259 aa). FASTA: opt: 585 Z-score: 707.5 E(): 1.6e-31 Smith-Waterman score: 680; 46.743 identity in 261 aa overlap ORF ftt0395; hypothetical protein 396253 4200447 FTF0395 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666572.1 395540 D 393115 CDS YP_666573.1 110670016 4200095 396386..398611 1 NC_008245.1 decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling; DNA topoisomerase IV subunit A 398611 parC 4200095 parC Francisella tularensis subsp. tularensis FSC198 DNA topoisomerase IV subunit A YP_666573.1 396386 D 393115 CDS YP_666574.1 110670017 4200103 398611..399297 1 NC_008245.1 enables the cleavage of the glycosidic bond in both 5'-methylthioadenosine and S-adenosylhomocysteine; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 399297 mtn 4200103 mtn Francisella tularensis subsp. tularensis FSC198 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase YP_666574.1 398611 D 393115 CDS YP_666575.1 110670018 4200448 complement(399284..399883) 1 NC_008245.1 ORF ftt0398c; hypothetical protein 399883 4200448 FTF0398c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666575.1 399284 R 393115 CDS YP_666576.1 110670019 4200449 complement(399876..400994) 1 NC_008245.1 Similar to Q885D8 BNR/Asp-box repeat protein from Pseudomonas syringae (432 aa). FASTA: opt: 536 Z-score: 654.4 E(): 1.5e-28 Smith-Waterman score: 536; 31.922 identity in 307 aa overlap ORF ftt0399c; BNR/Asp-box repeat-containing protein 400994 4200449 FTF0399c Francisella tularensis subsp. tularensis FSC198 BNR/Asp-box repeat-containing protein YP_666576.1 399876 R 393115 CDS YP_666577.1 110670020 4200744 401193..403169 1 NC_008245.1 Similar to Q88L07 Soluble lytic transglycosylase,putative from Pseudomonas putida (657 aa). FASTA: opt: 596 Z-score: 646.0 E(): 3.9e-28 Smith-Waterman score: 819; 27.822 identity in 629 aa overlap.; soluble lytic murein transglycosylase 403169 slt 4200744 slt Francisella tularensis subsp. tularensis FSC198 soluble lytic murein transglycosylase YP_666577.1 401193 D 393115 CDS YP_666578.1 110670021 4200450 403459..404181 1 NC_008245.1 Partial homology to carboxy terminus of Q9CLV4a Hypothetical protein PM1097 from Pasteurella multocida (417 aa). FASTA: opt: 269 Z-score: 330.3 E(): 1.7e-10 Smith-Waterman score: 269; 26.050identity in 238 aa overlap ORF ftt0401; hypothetical protein 404181 4200450 FTF0401 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666578.1 403459 D 393115 CDS YP_666579.1 110670022 4199352 404538..408017 1 NC_008245.1 Similar to DP3A_SALTY (P14567) DNA polymerase III alpha subunit from Salmonella typhimurium (1160 aa). FASTA: opt: 3099 Z-score: 3288.3 E(): 2.6e-175 Smith-Waterman score: 3099; 44.781identity in 1121 aa overlap.; DNA polymerase III subunit alpha 408017 dnaE 4199352 dnaE Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit alpha YP_666579.1 404538 D 393115 CDS YP_666580.1 110670023 4199534 408093..408728 1 NC_008245.1 Similar to DEF_LEPIN (Q93LE9) Peptide deformylase from Leptospira interrogans (178 aa). FASTA: opt: 312 Z-score: 399.2 E(): 2.4e-14 Smith-Waterman score: 312; 35.758 identity in 165 aa overlap; peptide deformylase 408728 def1 4199534 def1 Francisella tularensis subsp. tularensis FSC198 peptide deformylase YP_666580.1 408093 D 393115 CDS YP_666581.1 110670024 4199560 409432..410694 1 NC_008245.1 Similar to Q83CV1 Putative lipoprotein ABC transporter,permease protein from Coxiella burnetii (414 aa). FASTA: opt: 1273 Z-score: 1436.4 E(): 4.1e-72 Smith-Waterman score: 1273; 48.104 identity in 422 aa overlap; lipoprotein releasing system, subunit C,putative membrane protein 410694 lolC 4199560 lolC Francisella tularensis subsp. tularensis FSC198 lipoprotein releasing system, subunit C,putative membrane protein YP_666581.1 409432 D 393115 CDS YP_666582.1 110670025 4199561 410687..411382 1 NC_008245.1 Similar to LOLD_NEIMA (P57030) Lipoprotein releasing system ATP-binding transport protein from Neisseria meningitidis (231 aa). FASTA: opt: 791 Z-score: 892.8 E(): 7e-42 Smith-Waterman score: 791; 51.770 identity in 226 aa overlap; lipoprotein releasing system, subunit D, ABC transporter ATP-binding protein 411382 lolD 4199561 lolD Francisella tularensis subsp. tularensis FSC198 lipoprotein releasing system, subunit D, ABC transporter ATP-binding protein YP_666582.1 410687 D 393115 CDS YP_666583.1 110670026 4200312 411499..413640 1 NC_008245.1 Similar to DCLY_ECOLI (P23892) Lysine decarboxylase, inducible from E. coli (715 aa) FASTA: opt: 2608 Z-score: 3095.0 E(): 1.7e-164 Smith-Waterman score: 2608; 53.912 identity in 703 aa overlap; lysine decarboxylase, inducable 413640 cadA 4200312 cadA Francisella tularensis subsp. tularensis FSC198 lysine decarboxylase, inducable YP_666583.1 411499 D 393115 CDS YP_666584.1 110670027 4199281 413732..414808 1 NC_008245.1 catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein; glycine cleavage system aminomethyltransferase T 414808 gcvT 4199281 gcvT Francisella tularensis subsp. tularensis FSC198 glycine cleavage system aminomethyltransferase T YP_666584.1 413732 D 393115 CDS YP_666585.1 110670028 4199277 414864..415247 1 NC_008245.1 Similar to Q8FE66 Glycine cleavage system H protein from E coli (130 aa). FASTA: opt: 526 Z-score: 699.9 E(): 4.3e-31 Smith-Waterman score: 526; 60.938 identity in 128 aa overlap; glycine cleavage system H protein 415247 gcvH1 4199277 gcvH1 Francisella tularensis subsp. tularensis FSC198 glycine cleavage system H protein YP_666585.1 414864 D 393115 CDS YP_666586.1 110670029 4199279 415311..416678 1 NC_008245.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 2 to form the P protein; glycine dehydrogenase subunit 1 416678 gcvP1 4199279 gcvP1 Francisella tularensis subsp. tularensis FSC198 glycine dehydrogenase subunit 1 YP_666586.1 415311 D 393115 CDS YP_666587.1 110670030 4199280 416684..418129 1 NC_008245.1 acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 1 to form the P protein; glycine dehydrogenase subunit 2 418129 gcvP2 4199280 gcvP2 Francisella tularensis subsp. tularensis FSC198 glycine dehydrogenase subunit 2 YP_666587.1 416684 D 393115 CDS YP_666588.1 110670031 4199436 complement(418249..419094) 1 NC_008245.1 Similar to Q8FQC7 Putative shikimate 5-dehydrogenase from Corynebacterium efficiens(269 aa). FASTA: opt: 283 Z-score: 321.7 E(): 4.6e-10 Smith-Waterman score: 287; 28.077 identity in 260 aa overlap.; shikimate 5-dehydrogenase 419094 aroE2 4199436 aroE2 Francisella tularensis subsp. tularensis FSC198 shikimate 5-dehydrogenase YP_666588.1 418249 R 393115 CDS YP_666589.1 110670032 4199950 complement(419120..422332) 1 NC_008245.1 Similar to Q8XK16 Pullulanase from Clostridium perfringens (1064 aa). FASTA: opt: 3419 Z-score: 3958.4 E(): 1.4e-212 Smith-Waterman score: 3419; 48.369identity in 1042 aa overlap Other FASTA hits have lower scores due to generally being smaller in size. Q8XK16 is an electronic annotation; pullulonase 422332 pulB 4199950 pulB Francisella tularensis subsp. tularensis FSC198 pullulonase YP_666589.1 419120 R 393115 CDS YP_666590.1 110670033 4199783 complement(422366..424288) 1 NC_008245.1 catalyzes the transfer of a segment of a 1,4-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain; glycogen branching protein 424288 glgB 4199783 glgB Francisella tularensis subsp. tularensis FSC198 glycogen branching protein YP_666590.1 422366 R 393115 CDS YP_666591.1 110670034 4199244 424590..426224 1 NC_008245.1 catalyzes the interconversion of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate; phosphoglucomutase 426224 pgm 4199244 pgm Francisella tularensis subsp. tularensis FSC198 phosphoglucomutase YP_666591.1 424590 D 393115 CDS YP_666592.1 110670035 4199782 427540..429009 1 NC_008245.1 catalyzes the formation of alpha-1,4-glucan chains from ADP-glucose; glycogen synthase 429009 glgA 4199782 glgA Francisella tularensis subsp. tularensis FSC198 glycogen synthase YP_666592.1 427540 D 393115 CDS YP_666593.1 110670036 4199658 429114..431387 1 NC_008245.1 Similar to Q8DN49 Maltodextrin phosphorylase from Streptococcus pneumoniae (752 aa). FASTA: opt: 2938 Z-score: 3443.0 E(): 6.9e-184 Smith-Waterman score: 2938; 59.569 identity in 742 aa overlap; maltodextrin phosphorylase 431387 malP 4199658 malP Francisella tularensis subsp. tularensis FSC198 maltodextrin phosphorylase YP_666593.1 429114 D 393115 CDS YP_666594.1 110670037 4199657 431431..432894 1 NC_008245.1 amylomaltase; acts to release glucose from maltodextrins; 4-alpha-glucanotransferase 432894 malA 4199657 malA Francisella tularensis subsp. tularensis FSC198 4-alpha-glucanotransferase YP_666594.1 431431 D 393115 CDS YP_666595.1 110670038 4200289 432968..433876 1 NC_008245.1 glycine--tRNA ligase alpha chain; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA; glycyl-tRNA synthetase subunit alpha 433876 glyQ 4200289 glyQ Francisella tularensis subsp. tularensis FSC198 glycyl-tRNA synthetase subunit alpha YP_666595.1 432968 D 393115 CDS YP_666596.1 110670039 4200108 434087..435526 1 NC_008245.1 Similar to MURE_NEIMB (Q9K0Y9) UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase from Neisseria meningitidis (492 aa). FASTA: opt: 1037 Z-score: 1223.7, E(): 2.9e-60 Smith-Waterman score: 1037; 40.795 identity in 478 aa overlap; UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase 435526 murE 4200108 murE Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase YP_666596.1 434087 D 393115 CDS YP_666597.1 110670040 4200109 436318..437676 1 NC_008245.1 Similar to Q87SG8 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate -D-alanyl-D-alanyl ligase from Vibrio parahaemolyticus (454 aa). FASTA: opt: 870 Z-score: 1005.8 E(): 3.9e-48 Smith-Waterman score: 870; 35.011 identity in 457 aa overlap deleted EC_number 6.3.2.15; UDP-N--acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase 437676 murF 4200109 murF Francisella tularensis subsp. tularensis FSC198 UDP-N--acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase YP_666597.1 436318 D 393115 CDS YP_666598.1 110670041 4200452 437685..437864 1 NC_008245.1 ORF ftt0423; hypothetical protein 437864 4200452 FTF0423 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666598.1 437685 D 393115 CDS YP_666599.1 110670042 4200453 437995..438504 1 NC_008245.1 ORF ftt0424; hypothetical protein 438504 4200453 FTF0424 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666599.1 437995 D 393115 CDS YP_666600.1 110670043 4199688 complement(438560..439654) 1 NC_008245.1 catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde; aspartate-semialdehyde dehydrogenase 439654 asd 4199688 asd Francisella tularensis subsp. tularensis FSC198 aspartate-semialdehyde dehydrogenase YP_666600.1 438560 R 393115 CDS YP_666601.1 110670044 4199141 442964..444250 1 NC_008245.1 catalyzes the formation of L-threonine from O-phospho-L-homoserine; threonine synthase 444250 thrC1 4199141 thrC1 Francisella tularensis subsp. tularensis FSC198 threonine synthase YP_666601.1 442964 D 393115 CDS YP_666602.1 110670045 4199082 444891..445325 1 NC_008245.1 Similar to SPEH_METJA (Q57763) S-adenosylmethionine decarboxylase proenzyme from Methanococcus jannaschii (124 aa). FASTA: opt: 355 Z-score: 487.2, E(): 3e-19 Smith-Waterman score: 355; 44.737 identity in 114 aa overlap; S-adenosylmethionine decarboxylase 445325 speH 4199082 speH Francisella tularensis subsp. tularensis FSC198 S-adenosylmethionine decarboxylase YP_666602.1 444891 D 393115 CDS YP_666603.1 110670046 4199081 445325..446194 1 NC_008245.1 catalyzes the formation of spermidine from putrescine and S-adenosylmethioninamine; spermidine synthase 446194 speE 4199081 speE Francisella tularensis subsp. tularensis FSC198 spermidine synthase YP_666603.1 445325 D 393115 CDS YP_666604.1 110670047 4199080 446220..447629 1 NC_008245.1 Similar to Q8A2B1 Putative arginine decarboxylase from Bacteroides thetaiotamicron (630 aa). FASTA: opt: 571 Z-score: 678.2 E(): 6.9e-30 Smith-Waterman score: 689; 28.755 identity in 546 aa overlap; putative arginine decarboxylase 447629 speA 4199080 speA Francisella tularensis subsp. tularensis FSC198 putative arginine decarboxylase YP_666604.1 446220 D 393115 CDS YP_666605.1 110670048 4200454 447679..447867 1 NC_008245.1 ORF ftt0433; hypothetical protein 447867 4200454 FTF0433 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666605.1 447679 D 393115 CDS YP_666606.1 110670049 4200455 447848..448834 1 NC_008245.1 Similar to Q8KCB6 Hypothetical protein CT1508 from Chlorobium tepidum (347 aa). FASTA: opt: 742 Z-score: 939.4 E(): 2e-44 Smith-Waterman score: 837; 38.484 identity in 343 aa overlap PAD_porph is a possible virulence factor for P. gingivalis: PMID: 10377098 ORF ftt0434; hypothetical protein 448834 4200455 FTF0434 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666606.1 447848 D 393115 CDS YP_666607.1 110670050 4200456 448836..449696 1 NC_008245.1 Similar to Q87NU4 Putative carbon-nitrogen hydrolase from Vibrio parahaemolyticus (288 aa). FASTA: opt: 1139 Z-score: 1419.0 E(): 3.8e-71 Smith-Waterman score: 1139; 57.971 identity in 276 aa overlap. Possible candidate for citrulline ureidase ORF ftt0435; carbon-nitrogen hydrolase family protein 449696 4200456 FTF0435 Francisella tularensis subsp. tularensis FSC198 carbon-nitrogen hydrolase family protein YP_666607.1 448836 D 393115 CDS YP_666608.1 110670051 4199649 complement(449713..450429) 1 NC_008245.1 catalyzes the formation of 2,3=diacylglucosamine 1-phosphate from UDP-2,3=diacylglucosamine; UDP-2,3-diacylglucosamine hydrolase 450429 lpxH 4199649 lpxH Francisella tularensis subsp. tularensis FSC198 UDP-2,3-diacylglucosamine hydrolase YP_666608.1 449713 R 393115 CDS YP_666609.1 110670052 4199132 complement(450419..451045) 1 NC_008245.1 involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate; orotate phosphoribosyltransferase 451045 pyrE 4199132 pyrE Francisella tularensis subsp. tularensis FSC198 orotate phosphoribosyltransferase YP_666609.1 450419 R 393115 CDS YP_666610.1 110670053 4199851 451205..452575 1 NC_008245.1 Similar to MPL_ECOLI (P37773) UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso- diamino pimelate ligase (murein peptide ligase) from E coli (457 aa). FASTA: opt: 1392, Z-score: 1656.5, E(): 2.3e-84,Smith-Waterman score: 1392; 47.912 identity in 455 aa overlap; UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase 452575 mpl 4199851 mpl Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase YP_666610.1 451205 D 393115 CDS YP_666611.1 110670054 4199029 452572..453228 1 NC_008245.1 Similar to YJFH_ECOLI (P39290) hypothetical tRNA/rRNA methyltransferase yjfH from E. coli (243 aa). FASTA: opt: 556, Z-score: 695.5, E(): 7.5e-31 Smith-Waterman score: 556; 43.128 identity in 211 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element close to the C-terminal. It is not clear whether this insertion affects the function of the protein; tRNA/rRNA methyltransferase 453228 yjfH 4199029 yjfH Francisella tularensis subsp. tularensis FSC198 tRNA/rRNA methyltransferase YP_666611.1 452572 D 393115 CDS YP_666612.1 110670055 4199629 complement(join(453302..453760,453760..454086)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 454086 isftu1 4199629 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666612.1 453302 R 393115 CDS YP_666613.1 110670056 4199010 complement(454957..456150) 1 NC_008245.1 Similar to Q8EU72 Bicyclomycin resistance protein from Oceanobacillus iheyensis (412 aa). FASTA: opt: 614 Z-score: 668.2 E(): 2.5e-29 Smith-Waterman score: 614; 30.179identity in 391 aa overlap ORF ftt0442c; major facilitator transporter 456150 4199010 FTF0442c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666613.1 454957 R 393115 CDS YP_666614.1 110670057 4199011 456422..457465 1 NC_008245.1 Similar to Q83C03 Conserved domain protein from Coxiella burnetii (377 aa). FASTA: opt: 359 Z-score: 409.9 E(): 6.1e-15 Smith-Waterman score: 380; 27.350 identity in 351 aa overlap ORF ftt0443; hypothetical protein 457465 4199011 FTF0443 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666614.1 456422 D 393115 CDS YP_666615.1 110670058 4199137 457536..458774 1 NC_008245.1 Similar to Q83CB9 Multidrug transporter, putative from Coxiella burnetii (412 aa). FASTA: opt: 558 Z-score: 638.9 E(): 1.1e-27 Smith-Waterman score: 558; 25.854identity in 410 aa overlap. Similar to tetA and tetC which are situated on a plasmid in E. coli; multidrug transporter (tetracycline resistance protein) 458774 tet 4199137 tet Francisella tularensis subsp. tularensis FSC198 multidrug transporter (tetracycline resistance protein) YP_666615.1 457536 D 393115 CDS YP_666616.1 110670059 4199012 460705..462147 1 NC_008245.1 Similar to Q83RB7 Putative transport protein from Shigella flexneri (500 aa). FASTA: opt: 567 Z-score: 573.2 E(): 4.9e-24 Smith-Waterman score: 578; 26.122 identity in 490 aa overlap ORF ftt0446; proton-dependent oligopeptide transport (POT) family protein 462147 4199012 FTF0446 Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_666616.1 460705 D 393115 CDS YP_666617.1 110670060 4199013 complement(462291..463631) 1 NC_008245.1 Similar to Q83DW3 Hypothetical protein from Coxiella burnetii (497 aa). FASTA: opt: 334 Z-score: 356.6 E(): 5.7e-12 Smith-Waterman score: 336; 24.129 identity in 402 aa overlap ORF ftt0447c; hypothetical protein 463631 4199013 FTF0447c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666617.1 462291 R 393115 CDS YP_666618.1 110670061 4199790 complement(463652..465298) 1 NC_008245.1 catalyzes a two-step reaction, first charging a glutamine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; glutaminyl-tRNA synthetase 465298 glnS 4199790 glnS Francisella tularensis subsp. tularensis FSC198 glutaminyl-tRNA synthetase YP_666618.1 463652 R 393115 CDS YP_666619.1 110670062 4200097 465443..467035 1 NC_008245.1 PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP; phosphoenolpyruvate carboxykinase 467035 pckA 4200097 pckA Francisella tularensis subsp. tularensis FSC198 phosphoenolpyruvate carboxykinase YP_666619.1 465443 D 393115 CDS YP_666620.1 110670063 4199853 467110..468207 1 NC_008245.1 First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan; phospho-N-acetylmuramoyl-pentapeptide- transferase 468207 mraY 4199853 mraY Francisella tularensis subsp. tularensis FSC198 phospho-N-acetylmuramoyl-pentapeptide- transferase YP_666620.1 467110 D 393115 CDS YP_666621.1 110670064 4200107 468207..469457 1 NC_008245.1 Similar to MURD_CHLCV (Q821S1) UDP-N-acetylmuramoylalanine--D-glutamate ligase from Chlamydophila caviae (419 aa) FASTA: opt: 516 Z-score: 615.2 E(): 2.2e-26 Smith-Waterman score: 549; 28.000 identity in 425 aa overlap; UDP-N-acetylmuramoylalanine--D-glutamate ligase 469457 murD 4200107 murD Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylmuramoylalanine--D-glutamate ligase YP_666621.1 468207 D 393115 CDS YP_666622.1 110670065 4199197 469467..470672 1 NC_008245.1 Similar to Q9KPG6 Cell division protein FtsW from Vibrio cholerae (398 aa). FASTA: opt: 1114 Z-score: 1237.3 E(): 5e-61 Smith-Waterman score: 1114; 44.892 identity in 372 aa overlap; cell division protein FtsW 470672 ftsW 4199197 ftsW Francisella tularensis subsp. tularensis FSC198 cell division protein FtsW YP_666622.1 469467 D 393115 CDS YP_666623.1 110670066 4199014 complement(470680..471486) 1 NC_008245.1 Similar to Q83DW2 Hypothetical protein from Coxiella burnetii (273 aa). FASTA: opt: 554 Z-score: 648.8 E(): 3e-28 Smith-Waterman score: 554; 37.407identity in 270 aa overlap ORF ftt0453c; hypothetical protein 471486 4199014 FTF0453c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666623.1 470680 R 393115 CDS YP_666624.1 110670067 4199098 471585..472541 1 NC_008245.1 Similar to Q83DW4 Glycosyl transferase, group 2 family protein from Coxiella burnetii (324 aa). FASTA: opt: 896 Z-score: 1055.7 E(): 6.5e-51 Smith-Waterman score: 896; 43.189identity in 301 aa overlap; glycosyl transferase family protein 472541 yfdH 4199098 yfdH Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_666624.1 471585 D 393115 CDS YP_666625.1 110670068 4199015 complement(472933..474696) 1 NC_008245.1 Similar to Q8XQM9 Probable transmembrane protein from Ralstonia solanacearum (551 aa). FASTA: opt: 683 Z-score: 749.4 E(): 7.6e-34 Smith-Waterman score: 683; 29.174 identity in 545 aa overlap ORF ftt0455c; dolichyl-phosphate-mannose-protein mannosyltransferase family protein 474696 4199015 FTF0455c Francisella tularensis subsp. tularensis FSC198 dolichyl-phosphate-mannose-protein mannosyltransferase family protein YP_666625.1 472933 R 393115 CDS YP_666626.1 110670069 4199016 complement(474788..475081) 1 NC_008245.1 Similar to Y941_COXBU Hypothetical UPF0269 protein CBU0941 from Coxiella burnetii (90 aa). FASTA: opt: 346 Z-score: 470.4 E(): 2.6e-18 Smith-Waterman score: 346; 55.422 identity in 83 aa overlap ORF ftt0456c; hypothetical protein 475081 4199016 FTF0456c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666626.1 474788 R 393115 CDS YP_666627.1 110670070 4199096 complement(475072..475386) 1 NC_008245.1 Similar to Q9CNM6 Hypothetical protein PM0401 from Pasteurella multocida (109 aa). initn: 382 init1: 382 opt: 382 Z-score: 511.4 bits: 99.7 E(): 1.4e-20 Smith-Waterman score: 382; 58.586identity (58.58639ngapped) in 99 aa overlap; anaerobic sulfite reductase subunit 475386 yccK 4199096 yccK Francisella tularensis subsp. tularensis FSC198 anaerobic sulfite reductase subunit YP_666627.1 475072 R 393115 CDS YP_666628.1 110670071 4199085 475472..476104 1 NC_008245.1 Similar to SSPA_HAESO (P31784) Stringent starvation protein A homolog from Haemophilus somnus (212 aa). FASTA: opt: 377 Z-score: 473.8 E(): 1.7e-18 Smith-Waterman score: 377; 35.106 identity in 188 aa overlap; stringent starvation protein A 476104 sspA 4199085 sspA Francisella tularensis subsp. tularensis FSC198 stringent starvation protein A YP_666628.1 475472 D 393115 CDS YP_666629.1 110670072 4199079 476116..477135 1 NC_008245.1 SohB; periplasmic protein; member of the peptidase S49 family; putative periplasmic protease 477135 sohB 4199079 sohB Francisella tularensis subsp. tularensis FSC198 putative periplasmic protease YP_666629.1 476116 D 393115 CDS YP_666630.1 110670073 4200198 477143..478054 1 NC_008245.1 Similar to Q8D3A6 HolB protein from Wigglesworthia glossinidia brevipalpis (329 aa). FASTA: opt: 315 Z-score: 378.8 E(): 3.3e-13 Smith-Waterman score: 315; 27.304 identity in 293 aa overlap; DNA polymerase III, delta prime subunit 478054 holB 4200198 holB Francisella tularensis subsp. tularensis FSC198 DNA polymerase III, delta prime subunit YP_666630.1 477143 D 393115 CDS YP_666631.1 110670074 4199104 478065..478343 1 NC_008245.1 Similar to Q87LZ3 Putative RNA-binding protein containing KH domain from Vibrio parahaemolyticus (98 aa). FASTA: opt: 289 Z-score: 405.9 E(): 1e-14 Smith-Waterman score: 289; 52.222 identity in 90 aa overlap; RNA-binding protein 478343 yhbY 4199104 yhbY Francisella tularensis subsp. tularensis FSC198 RNA-binding protein YP_666631.1 478065 D 393115 CDS YP_666632.1 110670075 4200371 478351..479325 1 NC_008245.1 catalyzes the formation of porphobilinogen from 5-aminolevulinate; delta-aminolevulinic acid dehydratase 479325 hemB 4200371 hemB Francisella tularensis subsp. tularensis FSC198 delta-aminolevulinic acid dehydratase YP_666632.1 478351 D 393115 CDS YP_666633.1 110670076 4199107 479329..479796 1 NC_008245.1 Similar to Q8E8X2 RNA methyltransferase, TrmH family, group 2 from Shewanella oneidensis (154 aa). FASTA: opt: 569 Z-score: 759.8 E(): 2e-34 Smith-Waterman score: 569; 57.616 identity in 151 aa overlap; tRNA/rRNA methyltransferase 479796 yibK 4199107 yibK Francisella tularensis subsp. tularensis FSC198 tRNA/rRNA methyltransferase YP_666633.1 479329 D 393115 CDS YP_666634.1 110670077 4199425 479914..480978 1 NC_008245.1 Similar to ASG2_HAEIN (P43843) Probable L-asparaginase periplasmic [Precursor] from Haemophilus influenzae (349 aa). FASTA: opt: 897 Z-score: 1034.7 E(): 9.6e-50 Smith-Waterman score: 897; 43.966 identity in 348 aa overlap. No signal peptide predicted; periplasmic L-asparaginase II 480978 ansB 4199425 ansB Francisella tularensis subsp. tularensis FSC198 periplasmic L-asparaginase II YP_666634.1 479914 D 393115 CDS YP_666635.1 110670078 4199017 481702..481890 1 NC_008245.1 ORF ftt0465; hypothetical protein 481890 4199017 FTF0465 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666635.1 481702 D 393115 CDS YP_666636.1 110670079 4199430 complement(481942..483687) 1 NC_008245.1 catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase; arginyl-tRNA synthetase 483687 argS 4199430 argS Francisella tularensis subsp. tularensis FSC198 arginyl-tRNA synthetase YP_666636.1 481942 R 393115 CDS YP_666637.1 110670080 4200087 483813..486419 1 NC_008245.1 Similar to OSTA_XYLFT (Q87AI9) Organic solvent tolerance protein from Xylella fastidiosa (792 aa). FASTA: opt: 700 Z-score: 808.7 E(): 3.7e-37 Smith-Waterman score: 775; 26.572 identity in 636 aa overlap; organic solvent tolerance protein 486419 ostA1 4200087 ostA1 Francisella tularensis subsp. tularensis FSC198 organic solvent tolerance protein YP_666637.1 483813 D 393115 CDS YP_666638.1 110670081 4199093 486401..487822 1 NC_008245.1 Similar to Q8ZIK4 Survival protein SurA (Peptidyl-prolyl cis-trans isomerase) from Yersinia pestis (434 aa). FASTA: opt: 532 Z-score: 586.9 E(): 8.4e-25 Smith-Waterman score: 532; 25.176 identity in 425 aa overlap; peptidyl-prolyl cis-trans isomerase 487822 surA 4199093 surA Francisella tularensis subsp. tularensis FSC198 peptidyl-prolyl cis-trans isomerase YP_666638.1 486401 D 393115 CDS YP_666639.1 110670082 4199510 487824..488612 1 NC_008245.1 catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin; dimethyladenosine transferase 488612 ksgA 4199510 ksgA Francisella tularensis subsp. tularensis FSC198 dimethyladenosine transferase YP_666639.1 487824 D 393115 CDS YP_666640.1 110670083 4199426 488624..489451 1 NC_008245.1 hydrolyzes P(1),P(4)-bis(5'-adenosyl) tetraphosphate to form 2 ADP; diadenosine tetraphosphatase 489451 apaH 4199426 apaH Francisella tularensis subsp. tularensis FSC198 diadenosine tetraphosphatase YP_666640.1 488624 D 393115 CDS YP_666641.1 110670084 4199434 489455..489892 1 NC_008245.1 catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis; 3-dehydroquinate dehydratase 489892 aroD 4199434 aroD Francisella tularensis subsp. tularensis FSC198 3-dehydroquinate dehydratase YP_666641.1 489455 D 393115 CDS YP_666642.1 110670085 4199747 489897..490370 1 NC_008245.1 Similar to BCCP_PSEAE (P37799) Biotin carboxyl carrier protein of acetyl-CoA carboxylase from Pseudomonas aeruginosa (156 aa). FASTA: opt: 466 Z-score: 537.8 E(): 4.6e-22 Smith-Waterman score: 466; 48.148 identity in 162 aa overlap; Acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit 490370 accB 4199747 accB Francisella tularensis subsp. tularensis FSC198 Acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit YP_666642.1 489897 D 393115 CDS YP_666643.1 110670086 4199748 490435..491790 1 NC_008245.1 Similar to Q9KV62 Acetyl-CoA carboxylase, biotin carboxylase subunit from Vibrio cholerae (447 aa). FASTA: opt: 2119 Z-score: 2505.5 E(): 1.2e-131 Smith-Waterman score: 2119; 70.270 identity in 444 aa overlap; Acetyl-CoA carboxylase, biotin carboxylase subunit 491790 accC 4199748 accC Francisella tularensis subsp. tularensis FSC198 Acetyl-CoA carboxylase, biotin carboxylase subunit YP_666643.1 490435 D 393115 CDS YP_666644.1 110670087 4199018 491884..492564 1 NC_008245.1 ORF ftt0474; hypothetical protein 492564 4199018 FTF0474 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666644.1 491884 D 393115 CDS YP_666645.1 110670088 4200099 492590..493708 1 NC_008245.1 Similar to Q87G16 (Q87G16) Putative membrane protein from Vibrio parahaemolyticus (365 aa). FASTA: opt: 362 Z-score: 432.0 E(): 3.6e-16 Smith-Waterman score: 412; 29.167 identity in 384 aa overlap; mechanosensitive ion channel protein 493708 msc 4200099 msc Francisella tularensis subsp. tularensis FSC198 mechanosensitive ion channel protein YP_666645.1 492590 D 393115 CDS YP_666646.1 110670089 4199741 complement(493713..494636) 1 NC_008245.1 Similar to Q83AR3 (Q83AR3) Lysyl-tRNA synthetase-related protein from Coxiella burnetii (321 aa). FASTA: opt: 727 Z-score: 890.9 E(): 9.9e-42 Smith-Waterman score: 727; 42.088 identity in 297 aa overlap; lysyl-tRNA synthetase 494636 poxA 4199741 poxA Francisella tularensis subsp. tularensis FSC198 lysyl-tRNA synthetase YP_666646.1 493713 R 393115 CDS YP_666647.1 110670090 4200309 complement(494633..495415) 1 NC_008245.1 Similar to Q83CV0 BirA bifunctional protein from Coxiella burnetii (323 aa). FASTA: opt: 375 Z-score: 457.7 E(): 1.3e-17 Smith-Waterman score: 375; 31.004 identity in 229 aa overlap; transcription repressor BirA 495415 birA 4200309 birA Francisella tularensis subsp. tularensis FSC198 transcription repressor BirA YP_666647.1 494633 R 393115 CDS YP_666648.1 110670091 4200605 complement(495412..497154) 1 NC_008245.1 Similar to RECJ_HAEIN (P45112) single-stranded-DNA-specific exonuclease from Haemophilus influenzae (575 aa). FASTA: opt: 869 Z-score: 996.6 E(): 1.3e-47 Smith-Waterman score: 902; 32.028identity in 562 aa overlap; single-stranded-DNA-specific exonuclease 497154 recJ 4200605 recJ Francisella tularensis subsp. tularensis FSC198 single-stranded-DNA-specific exonuclease YP_666648.1 495412 R 393115 CDS YP_666649.1 110670092 4199241 complement(497148..498254) 1 NC_008245.1 Similar to Q9I4W6 Hypothetical protein PA1007 from Pseudomonas aeruginosa (357 aa). FASTA: opt: 1016 Z-score: 1092.5 E(): 5.8e-53 Smith-Waterman score: 1016; 40.698 identity in 344 aa overlap; PerM family protein 498254 perM 4199241 perM Francisella tularensis subsp. tularensis FSC198 PerM family protein YP_666649.1 497148 R 393115 CDS YP_666650.1 110670093 4200692 complement(498356..499765) 1 NC_008245.1 Similar to Q8YBJ1 Glutamate:gamma-aminobutyrate antiporter from Brucella melitensis (510 aa). FASTA: opt: 983 Z-score: 1079.5 E(): 3.1e-52 Smith-Waterman score: 983; 34.698 identity in 464 aa overlap; glutamate:gamma-aminobutyric acid antiporter family protein 499765 xasA 4200692 xasA Francisella tularensis subsp. tularensis FSC198 glutamate:gamma-aminobutyric acid antiporter family protein YP_666650.1 498356 R 393115 CDS YP_666651.1 110670094 4199737 499849..501030 1 NC_008245.1 Similar to Q92RX4 Probable putrescine-binding periplasmic protein from Rhizobium meliloti (364 aa). FASTA: opt: 847 Z-score: 993.2 E(): 2e-47 Smith-Waterman score: 847; 35.714 identity in 350 aa overlap; putrescine-binding periplasmic protein 501030 potF 4199737 potF Francisella tularensis subsp. tularensis FSC198 putrescine-binding periplasmic protein YP_666651.1 499849 D 393115 CDS YP_666652.1 110670095 4199019 complement(501283..502098) 1 NC_008245.1 ORF ftt0482c; lipoprotein 502098 4199019 FTF0482c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666652.1 501283 R 393115 CDS YP_666653.1 110670096 4199020 complement(502317..503204) 1 NC_008245.1 Similar to AAP95905 (AAP95905) Hypothetical protein from Haemophilus ducreyi 35000HP (342 aa). FASTA: opt: 686 Z-score: 815.5 E(): 1.6e-37 Smith-Waterman score: 686; 40.678 identity in 295 aa overlap ORF ftt0483c; hypothetical protein 503204 4199020 FTF0483c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666653.1 502317 R 393115 CDS YP_666654.1 110670097 4199021 503358..504077 1 NC_008245.1 ORF ftt0484; hypothetical protein 504077 4199021 FTF0484 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666654.1 503358 D 393115 CDS YP_666655.1 110670098 4199022 504145..504807 1 NC_008245.1 ORF ftt0485; hypothetical protein 504807 4199022 FTF0485 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666655.1 504145 D 393115 CDS YP_666656.1 110670099 4200112 504815..506617 1 NC_008245.1 Similar to MUTL_RICPR (Q9ZC88) DNA mismatch repair protein mutL from Rickettsia prowazekii (595 aa). FASTA: opt: 1236 Z-score: 1435.6 E(): 4.5e-72 Smith-Waterman score: 1236; 37.291 identity in 598 aa overlap; DNA mismatch repair protein 506617 mutL 4200112 mutL Francisella tularensis subsp. tularensis FSC198 DNA mismatch repair protein YP_666656.1 504815 D 393115 CDS YP_666657.1 110670100 4199023 506974..507708 1 NC_008245.1 Similar to Q83AI9 Membrane protein,putative from Coxiella burnetii (401 aa). FASTA: 306 opt: 370 Z-score: 417.4 E(): 2.3e-15 Smith-Waterman score: 370; 30.342 identity in 234 aa overlap ORF ftt0487; hypothetical protein 507708 4199023 FTF0487 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666657.1 506974 D 393115 CDS YP_666658.1 110670101 4199024 complement(507715..508848) 1 NC_008245.1 Similar to Q83BM0 Major facilitator family transporter from Coxiella burnetii (428 aa). FASTA: opt: 319 Z-score: E(): 3.7e-11 Smith-Waterman score: 319; 21.951 identity in 369 aa overlap ORF ftt0488c; major facilitator transporter 508848 4199024 FTF0488c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666658.1 507715 R 393115 CDS YP_666659.1 110670102 4198971 complement(508990..509940) 1 NC_008245.1 Similar to Q8ZGC9 Thioredoxin reductase from Yersinia pestis (320 aa). FASTA: opt: 1525 Z-score: 1798.2 E(): 2.9e-92 Smith-Waterman score: 1525; 70.927 identity in 313 aa overlap; thioredoxin reductase 509940 trxB 4198971 trxB Francisella tularensis subsp. tularensis FSC198 thioredoxin reductase YP_666659.1 508990 R 393115 CDS YP_666660.1 110670103 4199025 complement(510032..511249) 1 NC_008245.1 Similar Q888K0 Phospholipase D family protein from Pseudomonas syringae (pv. tomato) (422 aa). FASTA: opt: 440 Z-score: 514.4 E(): 9.2e-21 Smith-Waterman score: 440; 25.840 identity in 387 aa overlap ORF ftt0490c; phospholipase D 511249 4199025 FTF0490c Francisella tularensis subsp. tularensis FSC198 phospholipase D YP_666660.1 510032 R 393115 CDS YP_666661.1 110670104 4200293 complement(511292..511966) 1 NC_008245.1 Similar to Q82SZ4 Phosphoglycolate phosphatase from Nitrosomonas europaea (240 aa). FASTA: opt: 432 Z-score: 532.2 E(): 9.4e-22 Smith-Waterman score: 432; 33.486 identity in 218 aa overlap; phosphoglycolate phosphatase 511966 gph 4200293 gph Francisella tularensis subsp. tularensis FSC198 phosphoglycolate phosphatase YP_666661.1 511292 R 393115 CDS YP_666662.1 110670105 4199654 complement(512007..512933) 1 NC_008245.1 Similar to Q87J66 Transcriptional regulator, LysR family from Vibrio parahaemolyticus (311 aa). FASTA: opt: 675 Z-score: 768.6 E(): 6.4e-35 Smith-Waterman score: 675; 33.115 identity in 305 aa overlap; LysR family transcriptional regulator 512933 lysR 4199654 lysR Francisella tularensis subsp. tularensis FSC198 LysR family transcriptional regulator YP_666662.1 512007 R 393115 CDS YP_666663.1 110670106 4200281 complement(514232..514954) 1 NC_008245.1 Similar Q9CNA6 Hypothetical protein PM0526 from Pasteurella multocida (244 aa). FASTA: opt: 521 Z-score: 651.2 E(): 2.2e-28 Smith-Waterman score: 521; 40.175 identity in 229 aa overlap; CutC family protein 514954 cutC 4200281 cutC Francisella tularensis subsp. tularensis FSC198 CutC family protein YP_666663.1 514232 R 393115 CDS YP_666664.1 110670107 4199027 515287..515790 1 NC_008245.1 Similar to Q9K0Q8 Hypothetical protein NMB0526 from Neisseria meningitidis (serogroup B) (172 aa). FASTA: opt: 455 Z-score: 573.9 E(): 4.5e-24 Smith-Waterman score: 455; 42.353 identity in 170 aa overlap ORF ftt0495; hypothetical protein 515790 4199027 FTF0495 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666664.1 515287 D 393115 CDS YP_666665.1 110670108 4199028 515796..516560 1 NC_008245.1 Similar to Q935B8 Hypothetical protein HCM2.0044c from Salmonella typhi (251 aa). FASTA: opt: 242 Z-score: 297.7 E(): 1.1e-08 Smith-Waterman score: 296; 28.000 identity in 250 aa overlap ORF ftt0496; hypothetical protein 516560 4199028 FTF0496 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666665.1 515796 D 393115 CDS YP_666666.1 110670109 4199385 519151..519873 1 NC_008245.1 Similar to Q8ZA05 Hypothetical protein YPO4021 from Yersinia pestis (414 aa). FASTA: opt: 230 Z-score: 284.7 E(): 5.7e-08 Smith-Waterman score: 230; 24.779 identity in 226 aa overlap ORF ftt0499; hypothetical protein 519873 4199385 FTF0499 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666666.1 519151 D 393115 CDS YP_666667.1 110670110 4199386 520103..520363 1 NC_008245.1 ORF ftt0500; hypothetical protein 520363 4199386 FTF0500 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666667.1 520103 D 393115 CDS YP_666668.1 110670111 4199387 complement(520495..521535) 1 NC_008245.1 Similar to Q9I0I7 Hypothetical protein PA2651 from Pseudomonas aeruginosa (352 aa). FASTA: opt: 382 Z-score: 397.1 E(): 3.2e-14 Smith-Waterman score: 382; 26.686 identity in 341 aa overlap ORF ftt0501c; hypothetical protein 521535 4199387 FTF0501c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666668.1 520495 R 393115 CDS YP_666669.1 110670112 4199388 complement(521919..522344) 1 NC_008245.1 ORF ftt0502c; hypothetical protein 522344 4199388 FTF0502c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666669.1 521919 R 393115 CDS YP_666670.1 110670113 4199090 complement(522456..523328) 1 NC_008245.1 Similar to Q9JUS9 Putative succinyl-CoA synthetase alpha from Neisseria meningitidis (serogroup A) (296 aa). FASTA: opt: 1574 Z-score: 1817.5 E(): 2.4e-93 Smith-Waterman score: 1574; 81.661identity in 289 aa overlap; succinyl-CoA synthetase, alpha subunit 523328 sucD 4199090 sucD Francisella tularensis subsp. tularensis FSC198 succinyl-CoA synthetase, alpha subunit YP_666670.1 522456 R 393115 CDS YP_666671.1 110670114 4199089 complement(523360..524523) 1 NC_008245.1 catalyzes the interconversion of succinyl-CoA and succinate; succinyl-CoA synthetase subunit beta 524523 sucC 4199089 sucC Francisella tularensis subsp. tularensis FSC198 succinyl-CoA synthetase subunit beta YP_666671.1 523360 R 393115 CDS YP_666672.1 110670115 4199389 524693..526606 1 NC_008245.1 ORF ftt0505; hypothetical protein 526606 4199389 FTF0505 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666672.1 524693 D 393115 CDS YP_666673.1 110670116 4199390 complement(526614..526928) 1 NC_008245.1 ORF ftt0506c; hypothetical protein 526928 4199390 FTF0506c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666673.1 526614 R 393115 CDS YP_666674.1 110670117 4199391 527587..528354 1 NC_008245.1 Similar to Q9X610 BcfH from Salmonella typhimurium (269 aa). FASTA: opt: 305 Z-score: 371.4 E(): 8.5e-13 Smith-Waterman score: 305; 31.122 identity in 196 aa overlap ORF ftt0507; lipoprotein 528354 4199391 FTF0507 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666674.1 527587 D 393115 CDS YP_666675.1 110670118 4199362 complement(528361..529344) 1 NC_008245.1 Similar to Y926_SYNY3 (P72872) Hypothetical protein sll0926 from Synechocystis sp. (strain PCC 6803) (334 aa). FASTA: opt: 1129 Z-score: 1337.0 E(): 1.4e-66 Smith-Waterman score: 1129; 51.911 identity in 314 aa overlap; tRNA-dihydrouridine synthase A 529344 dusA 4199362 dusA Francisella tularensis subsp. tularensis FSC198 tRNA-dihydrouridine synthase A YP_666675.1 528361 R 393115 CDS YP_666676.1 110670119 4199392 complement(529378..530178) 1 NC_008245.1 Similar to Q8DBN3 Conserved hypothetical protein from Vibrio vulnificus (184 aa). FASTA: opt: 275 Z-score: 356.8 E(): 5.5e-12 Smith-Waterman score: 275; 30.208 identity in 192 aa overlap ORF ftt0509c; hypothetical protein 530178 4199392 FTF0509c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666676.1 529378 R 393115 CDS YP_666677.1 110670120 4200368 530354..532771 1 NC_008245.1 Similar to GYRB_SALTY (Q60008) DNA gyrase subunit B from Salmonella typhimurium (803 aa). FASTA: opt: 3370 Z-score: 3873.3 E(): 7.5e-208 Smith-Waterman score: 3370; 63.321 identity in 807 aa overlap; DNA gyrase subunit B 532771 gyrB 4200368 gyrB Francisella tularensis subsp. tularensis FSC198 DNA gyrase subunit B YP_666677.1 530354 D 393115 CDS YP_666678.1 110670121 4199393 532901..533764 1 NC_008245.1 with PdxT forms pyridoxal 5'-phosphate from glutamine, either ribose 5-phosphate or ribulose 5-phosphate, and either glyceraldehyde 3-phosphate or dihydroxyacetone phosphate; pyridoxal biosynthesis lyase PdxS 533764 4199393 FTF0511 Francisella tularensis subsp. tularensis FSC198 pyridoxal biosynthesis lyase PdxS YP_666678.1 532901 D 393115 CDS YP_666679.2 229560089 4199394 533767..534306 1 NC_008245.1 with PdxST is involved in the biosynthesis of pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of glutamine to glutamate and ammonia; PdxS utilizes the ammonia to synthesize pyridoxal 5'-phosphate; glutamine amidotransferase subunit PdxT 534306 4199394 FTF0512 Francisella tularensis subsp. tularensis FSC198 glutamine amidotransferase subunit PdxT YP_666679.2 533767 D 393115 CDS YP_666680.1 110670123 4199630 complement(join(534410..534868,534868..535194)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 535194 isftu1 4199630 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666680.1 534410 R 393115 CDS YP_666681.1 110670124 4199395 536411..536587 1 NC_008245.1 ORF ftt0515; hypothetical protein 536587 4199395 FTF0515 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666681.1 536411 D 393115 CDS YP_666682.1 110670125 4199942 541265..542110 1 NC_008245.1 Similar to Q9CLW2 PrmA (Ribosomal protein L11 methyltransferase) from Pasteurella multocida (293 aa). FASTA: opt: 572 Z-score: 685.5 E(): 2.7e-30 Smith-Waterman score: 580; 33.677 identity in 291 aa overlap; 50S ribosomal protein L11 methyltransferase 542110 prmA 4199942 prmA Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L11 methyltransferase YP_666682.1 541265 D 393115 CDS YP_666683.1 110670126 4199398 542100..543083 1 NC_008245.1 Similar to Q9KGG5 Transcriptional regulator involved in nitrogen regulation, NifR3/Smm1 family from Bacillus halodurans (334 aa). FASTA: opt: 1134 Z-score: 1336.1 E(): 1.6e-66 Smith-Waterman score: 1134; 52.484 identity in 322 aa overlap ORF ftt0519; Nif3 family protein 543083 4199398 FTF0519 Francisella tularensis subsp. tularensis FSC198 Nif3 family protein YP_666683.1 542100 D 393115 CDS YP_666684.1 110670127 4199399 543313..543906 1 NC_008245.1 ORF ftt0520; hypothetical protein 543906 4199399 FTF0520 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666684.1 543313 D 393115 CDS YP_666685.1 110670128 4199056 544433..545410 1 NC_008245.1 Similar to Q8EGY1 Type I restriction-modification system, M subunit, putative from Shewanella oneidensis (684 aa). FASTA: opt: 278 Z-score: 287.3 E(): 4.1e-08 Smith-Waterman score: 291; 31.081 identity in 222 aa overlap ORF ftt0522; hypothetical protein 545410 4199056 FTF0522 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666685.1 544433 D 393115 CDS YP_666686.1 110670129 4199057 545535..546761 1 NC_008245.1 Similar to Q89Z57 Type I restriction enzyme EcoAI specificity protein from Bacteroides thetaiotamicron (474 aa). FASTA: opt: 208 Z-score: 239.0 E(): 2e-05 Smith-Waterman score: 246; 24.363 identity in 353 aa overlap ORF ftt0523; hypothetical protein 546761 4199057 FTF0523 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666686.1 545535 D 393115 CDS YP_666687.1 110670130 4199058 547003..547389 1 NC_008245.1 Similar to Q8KBB0 Hypothetical protein CT1879 from Chlorobium tepidum (193 aa). FASTA: opt: 467 Z-score: 629.2 E(): 3.7e-27 Smith-Waterman score: 467; 60.656 identity in 122 aa overlap ORF ftt0524; hypothetical protein 547389 4199058 FTF0524 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666687.1 547003 D 393115 CDS YP_666688.1 110670131 4199059 547436..548092 1 NC_008245.1 Similar to RA50_THEVO DNA double-strand break repair rad50 ATPase from Thermoplasma volcanicum (895 aa). FASTA: opt: 188 Z-score: 215.6 E(): 0.0004 Smith-Waterman score: 188; 25.926 identity in 216 aa overlap. Similarity to N terminal domain ORF ftt0525; hypothetical protein 548092 4199059 FTF0525 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666688.1 547436 D 393115 CDS YP_666689.1 110670132 4199060 548098..548304 1 NC_008245.1 ORF ftt0526; hypothetical protein 548304 4199060 FTF0526 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666689.1 548098 D 393115 CDS YP_666690.1 110670133 4199061 548325..549128 1 NC_008245.1 ORF ftt0527; hypothetical protein 549128 4199061 FTF0527 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666690.1 548325 D 393115 CDS YP_666691.1 110670134 4199062 549125..549502 1 NC_008245.1 ORF ftt0528; hypothetical protein 549502 4199062 FTF0528 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666691.1 549125 D 393115 CDS YP_666692.1 110670135 4199063 complement(550577..551179) 1 NC_008245.1 Similar to Q97FB4 Intracellular protease/amidase related enzyme from Clostridium acetobutylicum (201 aa). FASTA: opt: 703 Z-score: 884.5 E(): 2.2e-41 Smith-Waterman score: 703; 51.531 identity in 196 aa overlap ORF ftt0530c; DJ-1/PfpI family protein 551179 4199063 FTF0530c Francisella tularensis subsp. tularensis FSC198 DJ-1/PfpI family protein YP_666692.1 550577 R 393115 CDS YP_666693.1 110670136 4200303 complement(553181..554413) 1 NC_008245.1 Similar to (glutaredoxin 3) Q8ZJM8 Glutaredoxin 3 from Yersinia pestis (82 aa). FASTA: opt: 181 Z-score: 263.7 E(): 8.5e-07 Smith-Waterman score: 181; 40.260 identity in 77 aa overlap,(ribonucleoside-diphosphate reductase) Q8QYZ8 Ribonucleoside-diphosphate reductase,beta subunit-like protein from Rana tigrina ranavirus (387 aa). FASTA: opt: 1124 Z-score: 1382.6 E(): 4.1e-69 Smith-Waterman score: 1124; 51.703 identity in 323 aa overlap; bifunctional gluaredoxin/ribonucleoside-diphosphate reductase subunit beta 554413 grxC 4200303 grxC Francisella tularensis subsp. tularensis FSC198 bifunctional gluaredoxin/ribonucleoside-diphosphate reductase subunit beta YP_666693.1 553181 R 393115 CDS YP_666694.1 110670137 4200301 complement(554420..554680) 1 NC_008245.1 Similar to GLR1_ECOLI (P00277) Glutaredoxin 1 from Escherichia coli (85 aa). FASTA: opt: 191 Z-score: 274.9 E(): 2e-07 Smith-Waterman score: 191; 40.000identity in 80 aa overlap; glutaredoxin 554680 grxA 4200301 grxA Francisella tularensis subsp. tularensis FSC198 glutaredoxin YP_666694.1 554420 R 393115 CDS YP_666695.1 110670138 4200242 complement(554684..556456) 1 NC_008245.1 Catalyzes the rate-limiting step in dNTP synthesis; ribonucleotide-diphosphate reductase subunit alpha 556456 nrdA 4200242 nrdA Francisella tularensis subsp. tularensis FSC198 ribonucleotide-diphosphate reductase subunit alpha YP_666695.1 554684 R 393115 CDS YP_666696.1 110670139 4199663 complement(556660..557619) 1 NC_008245.1 Catalyzes the reversible oxidation of malate to oxaloacetate; malate dehydrogenase 557619 mdh 4199663 mdh Francisella tularensis subsp. tularensis FSC198 malate dehydrogenase YP_666696.1 556660 R 393115 CDS YP_666697.1 110670140 4199065 557767..558282 1 NC_008245.1 ORF ftt0536; hypothetical protein 558282 4199065 FTF0536 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666697.1 557767 D 393115 CDS YP_666698.1 110670141 4199066 558298..558933 1 NC_008245.1 Similar to Q8PD67 Ubiquinone biosynthesis protein from Xanthomonas campestris (217 aa). FASTA: opt: 710 Z-score: 878.4 E(): 4.9e-41 Smith-Waterman score: 710; 51.887 identity in 212 aa overlap (2-211:8-217) ORF ftt0537; ubiquinone biosynthesis protein 558933 4199066 FTF0537 Francisella tularensis subsp. tularensis FSC198 ubiquinone biosynthesis protein YP_666698.1 558298 D 393115 CDS YP_666699.1 110670142 4199067 complement(558942..559511) 1 NC_008245.1 Similar to Q87LS3 Hypothetical protein from Vibrio parahaemolyticus (203 aa). FASTA: opt: 267 Z-score: 350.1 E(): 1.3e-11 Smith-Waterman score: 320; 31.795 identity in 195 aa overlap ORF ftt0538c; lipoprotein 559511 4199067 FTF0538c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666699.1 558942 R 393115 CDS YP_666700.1 110670143 4199069 complement(560336..561826) 1 NC_008245.1 ORF ftt0540c; hypothetical protein 561826 4199069 FTF0540c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666700.1 560336 R 393115 CDS YP_666701.1 110670144 4199034 complement(561881..562480) 1 NC_008245.1 Similar to YQAB_ECOLI (P77475) Hypothetical protein yqaB from Escherichia coli (188 aa). FASTA: opt: 336 Z-score: 422.2 E(): 1.3e-15 Smith-Waterman score: 336; 33.889 identity in 180 aa overlap; haloacid dehalogenase 562480 yqaB 4199034 yqaB Francisella tularensis subsp. tularensis FSC198 haloacid dehalogenase YP_666701.1 561881 R 393115 CDS YP_666702.1 110670145 4199071 563221..564597 1 NC_008245.1 ORF ftt0543; hypothetical protein 564597 4199071 FTF0543 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666702.1 563221 D 393115 CDS YP_666703.1 110670146 4199726 564703..565035 1 NC_008245.1 Similar to PHNA_ECOLI PhnA protein from Escherichia coli (111 aa). FASTA: opt: 521 Z-score: 674.6 E(): 1.1e-29 Smith-Waterman score: 521; 66.972identity in 109 aa overlap; phosphonoacetate hydrolase 565035 phnA 4199726 phnA Francisella tularensis subsp. tularensis FSC198 phosphonoacetate hydrolase YP_666703.1 564703 D 393115 CDS YP_666704.1 110670147 4199072 565041..565256 1 NC_008245.1 ORF ftt0545; hypothetical protein 565256 4199072 FTF0545 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666704.1 565041 D 393115 CDS YP_666705.1 110670148 4199073 565260..565871 1 NC_008245.1 ORF ftt0546; hypothetical protein 565871 4199073 FTF0546 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666705.1 565260 D 393115 CDS YP_666706.1 110670149 4199074 566226..566759 1 NC_008245.1 ORF ftt0547; hypothetical protein 566759 4199074 FTF0547 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666706.1 566226 D 393115 CDS YP_666707.1 110670150 4199358 566811..567503 1 NC_008245.1 Similar to Q83EK2 DNA polymerase III, epsilon subunit from Coxiella burnetii (232 aa). FASTA: opt: 699 Z-score: 870.8 E(): 1.3e-40 Smith-Waterman score: 699; 49.550 identity in 222 aa overlap; DNA polymerase III subunit epsilon 567503 dnaQ 4199358 dnaQ Francisella tularensis subsp. tularensis FSC198 DNA polymerase III subunit epsilon YP_666707.1 566811 D 393115 CDS YP_666708.1 110670151 4200139 567497..567970 1 NC_008245.1 Similar to Q87AM4 Peptidase (202 aa). FASTA: opt: 481 Z-score: 603.7 E(): 9.8e-26 Smith-Waterman score: 481; 50.000 identity in 142 aa overlap; D-alanyl-D-alanine carboxypeptidase 567970 vanY 4200139 vanY Francisella tularensis subsp. tularensis FSC198 D-alanyl-D-alanine carboxypeptidase YP_666708.1 567497 D 393115 CDS YP_666709.1 110670152 4199075 567930..569366 1 NC_008245.1 Similar to Q8EHZ3 TPR domain protein from Shewanella oneidensis (466 aa). FASTA: opt: 304 Z-score: 346.2 E(): 2.2e-11 Smith-Waterman score: 331; 23.333 identity) in 480 aa overlap ORF ftt0550; hypothetical protein 569366 4199075 FTF0550 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666709.1 567930 D 393115 CDS YP_666710.1 110670153 4200384 570799..572295 1 NC_008245.1 Similar to Q8P9Q7 Aldehyde dehydrogenase from Xanthomonas campestris (510 aa). FASTA: opt: 1972 Z-score: 2329.9 E(): 6.9e-122 Smith-Waterman score: 1972; 60.285 identity in 491 aa overlap ORF ftt0552; aldehyde dehydrogenase 572295 4200384 FTF0552 Francisella tularensis subsp. tularensis FSC198 aldehyde dehydrogenase YP_666710.1 570799 D 393115 CDS YP_666711.1 110670154 4200385 572300..573079 1 NC_008245.1 Similar to Q8EJ53 Conserved hypothetical protein from Shewanella oneidensis (267 aa). FASTA: opt: 859 Z-score: 1057.9 E(): 4.9e-51 Smith-Waterman score: 859; 52.809 identity in 267 aa overlap ORF ftt0553; hypothetical protein 573079 4200385 FTF0553 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666711.1 572300 D 393115 CDS YP_666712.1 110670155 4200386 573163..573435 1 NC_008245.1 Similar to Q99SH0 Hypothetical protein SAV2087 from Stafylococcus aureus (97 aa). FASTA: opt: 243 Z-score: 331.6 E(): 1.4e-10 Smith-Waterman score: 243; 45.455 identity in 77 aa overlap ORF ftt0554; hypothetical protein 573435 4200386 FTF0554 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666712.1 573163 D 393115 CDS YP_666713.1 110670156 4200387 573449..574180 1 NC_008245.1 Similar to Q930P5 Hypothetical protein RA0150 from Rhizobium meliloti (262 aa). FASTA: opt: 924 Z-score: 1048.2 E(): 1.7e-50 Smith-Waterman score: 924; 54.202 identity in 238 aa overlap. Some similarity to glutaredoxin ORF ftt0555; hypothetical protein 574180 4200387 FTF0555 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666713.1 573449 D 393115 CDS YP_666714.1 110670157 4200089 complement(574183..575052) 1 NC_008245.1 Similar to O06466 Oxidative stress transcriptional regulator from Xanthomonas campestris (313 aa). FASTA: opt: 707 Z-score: 842.2 E(): 5.1e-39 Smith-Waterman score: 707; 36.207 identity in 290 aa overlap; oxidative stress transcriptional regulator 575052 oxyR 4200089 oxyR Francisella tularensis subsp. tularensis FSC198 oxidative stress transcriptional regulator YP_666714.1 574183 R 393115 CDS YP_666715.1 110670158 4200388 575150..575674 1 NC_008245.1 Similar to Q92MY1 Hypothetical protein R02467 from Rhizobium meliloti (180 aa). FASTA: opt: 821 Z-score: 1055.0 E(): 7.2e-51 Smith-Waterman score: 821; 66.092 identity in 174 aa overlap ORF ftt0557; AhpC/TSA family protein 575674 4200388 FTF0557 Francisella tularensis subsp. tularensis FSC198 AhpC/TSA family protein YP_666715.1 575150 D 393115 CDS YP_666716.1 110670159 4200389 575677..576288 1 NC_008245.1 Similar to Q8XU62 Hypothetical protein RSc3331 from Ralstonia solanacearum (201 aa). FASTA: opt: 602 Z-score: 724.0 E(): 2e-32 Smith-Waterman score: 602; 48.020 identity in 202 aa overlap ORF ftt0558; short chain dehydrogenase 576288 4200389 FTF0558 Francisella tularensis subsp. tularensis FSC198 short chain dehydrogenase YP_666716.1 575677 D 393115 CDS YP_666717.1 110670160 4200269 complement(576305..576970) 1 NC_008245.1 Similar to KCY_PASMU (P57875) Cytidylate kinase from Pasteurella multocida (227 aa). FASTA: opt: 748 Z-score: 879.9 E(): 4.1e-41 Smith-Waterman score: 748; 53.425 identity in 219 aa overlap; cytidylate kinase 576970 cmk 4200269 cmk Francisella tularensis subsp. tularensis FSC198 cytidylate kinase YP_666717.1 576305 R 393115 CDS YP_666718.1 110670161 4200742 complement(576957..578009) 1 NC_008245.1 catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine; phosphoserine aminotransferase 578009 serC 4200742 serC Francisella tularensis subsp. tularensis FSC198 phosphoserine aminotransferase YP_666718.1 576957 R 393115 CDS YP_666719.1 110670162 4199631 join(578329..578655,578655..579113) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 579113 isftu1 4199631 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666719.1 578329 D 393115 CDS YP_666720.1 110670163 4199738 579495..580616 1 NC_008245.1 Similar to Q8EHF2 Polyamine ABC transporter,ATP-binding protein from Shewanella oneidensis (378 aa). FASTA: opt: 1366 Z-score: 1557.5 E(): 7.3e-79 Smith-Waterman score: 1366; 55.496 identity in 373 aa overlap; polyamine transporter, ABC transporter,ATP-binding protein 580616 potG 4199738 potG Francisella tularensis subsp. tularensis FSC198 polyamine transporter, ABC transporter,ATP-binding protein YP_666720.1 579495 D 393115 CDS YP_666721.1 110670164 4199739 580580..581497 1 NC_008245.1 Similar to Q8FJE8 Putrescine transport system permease protein from Escherichia coli (314 aa). FASTA: opt: 949 Z-score: 1040.4 E(): 4.7e-50 Smith-Waterman score: 949; 43.878 identity in 294 aa overlap; polyamine transporter, subunit H, ABC transporter, membrane protein 581497 potH 4199739 potH Francisella tularensis subsp. tularensis FSC198 polyamine transporter, subunit H, ABC transporter, membrane protein YP_666721.1 580580 D 393115 CDS YP_666722.1 110670165 4199740 581511..582317 1 NC_008245.1 Similar to Q8EHF0 Polyamine ABC transporter,permease protein from Shewanella oneidensis (271 aa). FASTA: opt: 873 Z-score: 1010.7 E(): 2.1e-48 Smith-Waterman score: 873; 46.241 identity in 266 aa overlap; polyamine transporter, subunit I, ABC transporter, membrane protein 582317 potI 4199740 potI Francisella tularensis subsp. tularensis FSC198 polyamine transporter, subunit I, ABC transporter, membrane protein YP_666722.1 581511 D 393115 CDS YP_666723.1 110670166 4199288 complement(582461..583204) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 583204 isftu2 4199288 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666723.1 582461 R 393115 CDS YP_666724.1 110670167 4200390 583350..583616 1 NC_008245.1 ORF ftt0566; hypothetical protein 583616 4200390 FTF0566 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666724.1 583350 D 393115 CDS YP_666725.1 110670168 4200392 585822..586472 1 NC_008245.1 Similar to Q26545 Phosphoserine phosphohydrolase-like protein trans-spliced from Schistosoma mansoni (223 aa). FASTA: opt: 319 Z-score: 380.8 E(): 2.5e-13 Smith-Waterman score: 319; 29.493 identity in 217 aa overlap. Similar to eukaryotic proteins. Historical HGT-event? ORF ftt0568; hypothetical protein 586472 4200392 FTF0568 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666725.1 585822 D 393115 CDS YP_666726.1 110670169 4200393 complement(586461..587483) 1 NC_008245.1 Similar to Q8P3T2 Integral membrane protein from Xanthomonas campestris (347 aa). FASTA: opt: 906 Z-score: 1071.1 E(): 9e-52 Smith-Waterman score: 906; 41.739 identity in 345 aa overlap ORF ftt0569c; hypothetical protein 587483 4200393 FTF0569c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666726.1 586461 R 393115 CDS YP_666727.1 110670170 4200394 587563..587961 1 NC_008245.1 ORF ftt0570; hypothetical protein 587961 4200394 FTF0570 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666727.1 587563 D 393115 CDS YP_666728.1 110670171 4200395 587973..588680 1 NC_008245.1 Partially similar to Q96F61 Hypothetical protein from Homo sapiens (409 aa). FASTA: opt: 403 Z-score: 516.3 E(): 7.3e-21 Smith-Waterman score: 403; 34.171 identity in 199 aa overlap ORF ftt0571; hypothetical protein 588680 4200395 FTF0571 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666728.1 587973 D 393115 CDS YP_666729.1 110670172 4200396 588805..590259 1 NC_008245.1 Similar to Q8Z269 Putative PTR2 family transport protein from Salmonella typhi (489 aa). FASTA: opt: 729 Z-score: 778.1 E(): 1.9e-35 Smith-Waterman score: 770; 30.524identity in 439 aa overlap ORF ftt0572; proton-dependent oligopeptide transport (POT) family protein 590259 4200396 FTF0572 Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_666729.1 588805 D 393115 CDS YP_666730.1 110670173 4199760 590310..591407 1 NC_008245.1 Similar to Q81EB9 Alanine racemase from Bacillus cereus (408 aa). FASTA: opt: 573 Z-score: 679.1 E(): 6.2e-30 Smith-Waterman score: 573; 29.863 identity in 365 aa overlap; alanine racemase 591407 alr 4199760 alr Francisella tularensis subsp. tularensis FSC198 alanine racemase YP_666730.1 590310 D 393115 CDS YP_666731.1 110670174 4199246 593182..594024 1 NC_008245.1 Similar to Q8KBW6 Prephenate dehydratase from chlorobium tepidum (280 aa). FASTA: opt: 624 Z-score: 792.7 E(): 2.9e-36 Smith-Waterman score: 624; 38.489 identity in 278 aa overlap; prephenate dehydratase 594024 pheA 4199246 pheA Francisella tularensis subsp. tularensis FSC198 prephenate dehydratase YP_666731.1 593182 D 393115 CDS YP_666732.1 110670175 4200398 594050..594718 1 NC_008245.1 Similar to Q894N2 Putative zinc metallopeptidase from Clostridium tetani (236 aa). FASTA: opt: 434 Z-score: 526.0 E(): 2.1e-21 Smith-Waterman score: 434; 36.245 identity in 229 aa overlap ORF ftt0576; hypothetical protein 594718 4200398 FTF0576 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666732.1 594050 D 393115 CDS YP_666733.1 110670176 4200726 594774..596147 1 NC_008245.1 Similar to Q8EEW4 L-serine dehydratase 1 from Shewanella oneidensis (458 aa). FASTA: opt: 1442 Z-score: 1806.4 E(): 1e-92 Smith-Waterman score: 1442; 48.922 identity in 464 aa overlap; L-serine dehydratase 1 596147 sdaA 4200726 sdaA Francisella tularensis subsp. tularensis FSC198 L-serine dehydratase 1 YP_666733.1 594774 D 393115 CDS YP_666734.1 110670177 4200277 596250..597473 1 NC_008245.1 Similar to Q8ZDZ4 Putative selenocysteine lyase from Yersinia pestis (406 aa). FASTA: opt: 1343 Z-score: 1645.0 E(): 9.9e-84 Smith-Waterman score: 1343; 48.507 identity in 402 aa overlap; selenocysteine lyase 597473 csdB 4200277 csdB Francisella tularensis subsp. tularensis FSC198 selenocysteine lyase YP_666734.1 596250 D 393115 CDS YP_666735.1 110670178 4200399 597494..597850 1 NC_008245.1 Similar to AAP95950 HesB family protein from Haemophilus ducreyi (107 aa). FASTA: opt: 288 Z-score: 392.8 E(): 5.5e-14 Smith-Waterman score: 288; 41.121 identity in 107 aa overlap ORF ftt0579; hesB family protein 597850 4200399 FTF0579 Francisella tularensis subsp. tularensis FSC198 hesB family protein YP_666735.1 597494 D 393115 CDS YP_666736.1 110670179 4200400 597864..598415 1 NC_008245.1 Similar to Q83BY3 Hypothetical protein from Coxiella burnetii (180 aa). FASTA: opt: 593 Z-score: 750.6 E(): 6.4e-34 Smith-Waterman score: 593; 48.045 identity in 179 aa overlap ORF ftt0580; hypothetical protein 598415 4200400 FTF0580 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666736.1 597864 D 393115 CDS YP_666737.1 110670180 4200270 598427..598915 1 NC_008245.1 Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA; phosphopantetheine adenylyltransferase 598915 coaD 4200270 coaD Francisella tularensis subsp. tularensis FSC198 phosphopantetheine adenylyltransferase YP_666737.1 598427 D 393115 CDS YP_666738.1 110670181 4199407 598920..599165 1 NC_008245.1 ferredoxin. Identical to Q47937 Ferredoxin from Francisella novicida. Similar to Q88AH4 Ferredoxin from pseudomonas syringae (83 aa). FASTA: opt: 431 Z-score: 563.2 E(): 1.8e-23 Smith-Waterman score: 431; 65.432 identity in 81 aa overlap; ferredoxin 599165 fdx 4199407 fdx Francisella tularensis subsp. tularensis FSC198 ferredoxin YP_666738.1 598920 D 393115 CDS YP_666739.1 110670182 4199419 599272..600453 1 NC_008245.1 No transmembrane helix predicted; outer membrane associated protein 600453 fopA1 4199419 fopA1 Francisella tularensis subsp. tularensis FSC198 outer membrane associated protein YP_666739.1 599272 D 393115 CDS YP_666740.1 110670183 4200457 601115..604486 1 NC_008245.1 ORF ftt0584; hypothetical protein 604486 4200457 FTF0584 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666740.1 601115 D 393115 CDS YP_666741.1 110670184 4200459 605855..606433 1 NC_008245.1 Similar to Q9YCL9 Hypothetical protein APE1239 from Aeropyrum pernix (221 aa). FASTA: opt: 238 Z-score: 303.8 E(): 5e-09 Smith-Waterman score: 238; 25.683 identity in 183 aa overlap. Weakly conserved. ORF ftt0586; hypothetical protein 606433 4200459 FTF0586 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666741.1 605855 D 393115 CDS YP_666742.1 110670185 4199289 complement(606857..607600) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 607600 isftu2 4199289 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666742.1 606857 R 393115 CDS YP_666743.1 110670186 4199431 607763..609040 1 NC_008245.1 Similar to AROA_VIBPA (Q87QX9) 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio parahaemolyticus (426 aa). FASTA: opt: 928 Z-score: 1157.4 E(): 1.4e-56 Smith-Waterman score: 928; 36.916 identity in 428 aa overlap; 3-phosphoshikimate 1-carboxyvinyltransferase 609040 aroA 4199431 aroA Francisella tularensis subsp. tularensis FSC198 3-phosphoshikimate 1-carboxyvinyltransferase YP_666743.1 607763 D 393115 CDS YP_666744.1 110670187 4200460 609043..609633 1 NC_008245.1 ORF ftt0589; hypothetical protein 609633 4200460 FTF0589 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666744.1 609043 D 393115 CDS YP_666745.1 110670188 4200766 609584..610066 1 NC_008245.1 Similar to RNH_PSEAE (Q9I2S9) Ribonuclease H from Pseudomonas aeruginosa (148 aa). FASTA: opt: 673 Z-score: 830.4 E(): 2.3e-38 Smith-Waterman score: 673; 62.937 identity in 143 aa overlap; ribonuclease H 610066 rnhA 4200766 rnhA Francisella tularensis subsp. tularensis FSC198 ribonuclease H YP_666745.1 609584 D 393115 CDS YP_666746.1 110670189 4199424 610068..611105 1 NC_008245.1 Similar to Q8EEG1 L-asparaginase I from Shewanella oneidensis (337 aa). FASTA: opt: 1041 Z-score: 1293.4 E(): 3.7e-64 Smith-Waterman score: 1041; 49.258identity in 337 aa overlap; L-asparaginase 611105 ansA 4199424 ansA Francisella tularensis subsp. tularensis FSC198 L-asparaginase YP_666746.1 610068 D 393115 CDS YP_666747.1 110670190 4200286 611176..611862 1 NC_008245.1 Similar to Q87AM3 Carbonic anhydrase from xylella fastidiosa (220 aa). FASTA: opt: 577 Z-score: 733.9 E(): 5.5e-33 Smith-Waterman score: 722; 45.536 identity in 224 aa overlap; carbonic anhydrase 611862 cynT 4200286 cynT Francisella tularensis subsp. tularensis FSC198 carbonic anhydrase YP_666747.1 611176 D 393115 CDS YP_666748.1 110670191 4200461 complement(612149..612754) 1 NC_008245.1 ORF ftt0593c; hypothetical protein 612754 4200461 FTF0593c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666748.1 612149 R 393115 CDS YP_666749.1 110670192 4200462 complement(612818..613690) 1 NC_008245.1 Similar to Q87N89 Hypothetical protein from Vibrio parahaemolyticus (344 aa). FASTA: opt: 706 Z-score: 859.6 E(): 5.5e-40 Smith-Waterman score: 706; 42.160 identity in 287 aa overlap ORF ftt0594c; hypothetical protein 613690 4200462 FTF0594c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666749.1 612818 R 393115 CDS YP_666750.1 110670193 4198963 complement(613699..613869) 1 NC_008245.1 Similar to Q88C68 Rubredoxin from Pseudomonas putida (55 aa). FASTA: opt: 334 Z-score: 465.9 E(): 4.6e-18 Smith-Waterman score: 334; 78.431 identity in 51 aa overlap; rubredoxin 613869 rubA 4198963 rubA Francisella tularensis subsp. tularensis FSC198 rubredoxin YP_666750.1 613699 R 393115 CDS YP_666751.1 110670194 4200463 complement(613956..614573) 1 NC_008245.1 ORF ftt0596c; hypothetical protein 614573 4200463 FTF0596c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666751.1 613956 R 393115 CDS YP_666752.1 110670195 4200464 614685..616079 1 NC_008245.1 Similar to Q9K5Z6 Hypothetical UPF0061 protein BH393 from Bacillus halodurans (492 aa). FASTA: opt: 1251 Z-score: 1519.2 E(): 1e-76 Smith-Waterman score: 1357; 48.233 identity in 481 aa overlap ORF ftt0597; hypothetical protein 616079 4200464 FTF0597 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666752.1 614685 D 393115 CDS YP_666753.1 110670196 4200465 complement(616111..617391) 1 NC_008245.1 Similar to Q8XLU3 Probable transmembrane symporter from Clostridium perfringens (435 aa). FASTA: opt: 881 Z-score: 961.9 E(): 1.1e-45 Smith-Waterman score: 881; 33.721identity in 430 aa overlap ORF ftt0598c; Sodium-dicarboxylate symporter family protein 617391 4200465 FTF0598c Francisella tularensis subsp. tularensis FSC198 Sodium-dicarboxylate symporter family protein YP_666753.1 616111 R 393115 CDS YP_666754.1 110670197 4200466 617587..618615 1 NC_008245.1 Similar to Q9AAR1 SIS domain protein from Caulobacter crescentus (358 aa). FASTA: opt: 864 Z-score: 1052.1 E(): 1e-50 Smith-Waterman score: 864; 42.353 identity in 340 aa overlap. Putative Glucosamine-fructose-6-phosphate aminotransferase ORF ftt0599; SIS domain-containing protein 618615 4200466 FTF0599 Francisella tularensis subsp. tularensis FSC198 SIS domain-containing protein YP_666754.1 617587 D 393115 CDS YP_666755.1 110670198 4200468 620200..620319 1 NC_008245.1 ORF ftt0601; hypothetical protein 620319 4200468 FTF0601 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666755.1 620200 D 393115 CDS YP_666756.1 110670199 4200469 complement(620946..622424) 1 NC_008245.1 ORF ftt0602c; hypothetical protein 622424 4200469 FTF0602c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666756.1 620946 R 393115 CDS YP_666757.1 110670200 4200470 622984..623163 1 NC_008245.1 ORF ftt0603; hypothetical protein 623163 4200470 FTF0603 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666757.1 622984 D 393115 CDS YP_666758.1 110670201 4200471 623681..624115 1 NC_008245.1 Similar to Q9CNR2 NhaP NA+/H+ antiporter Conserved hypothetical membrane protein from Pasteurella multocida (441 aa). FASTA: opt: 315 Z-score: 391.3 E(): 6.6e-14 Smith-Waterman score: 315; 39.850 identity in 133 aa overlap. Matches to a central part of an Nhap protein ORF ftt0604; hypothetical protein 624115 4200471 FTF0604 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666758.1 623681 D 393115 CDS YP_666759.1 110670202 4200472 complement(624301..624489) 1 NC_008245.1 ORF ftt0605c; hypothetical protein 624489 4200472 FTF0605c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666759.1 624301 R 393115 CDS YP_666760.1 110670203 4199303 625293..626510 1 NC_008245.1 catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 626510 ispG 4199303 ispG Francisella tularensis subsp. tularensis FSC198 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase YP_666760.1 625293 D 393115 CDS YP_666761.1 110670204 4200474 626525..627103 1 NC_008245.1 Similar to Q9KHC2 Hypothetical 27.0 kDa protein from secondary endosymbiont of Glycaspis brimblecombei (244 aa). FASTA: opt: 467 Z-score: 605.3 E(): 8e-26 Smith-Waterman score: 467; 44.751identity in 181 aa overlap ORF ftt0608; hypothetical protein 627103 4200474 FTF0608 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666761.1 626525 D 393115 CDS YP_666762.1 110670205 4200475 627254..629047 1 NC_008245.1 Similar to Q83F75 Peptidase, M24 family protein from Coxiella burnetii (597 aa). FASTA: opt: 1665 Z-score: 1963.9 E(): 1.7e-101 Smith-Waterman score: 1665; 45.286 identity in 594 aa overlap ORF ftt0609; peptidase, M24 family protein 629047 4200475 FTF0609 Francisella tularensis subsp. tularensis FSC198 peptidase, M24 family protein YP_666762.1 627254 D 393115 CDS YP_666763.1 110670206 4200476 629145..630209 1 NC_008245.1 Similar to Q8DFB3 DNA/RNA endonuclease G from Vibrio vulnificus (247 aa). FASTA: opt: 682 Z-score: 838.7 E(): 8e-39 Smith-Waterman score: 682; 46.311 identity in 244 aa overlap ORF ftt0610; DNA/RNA endonuclease family protein 630209 4200476 FTF0610 Francisella tularensis subsp. tularensis FSC198 DNA/RNA endonuclease family protein YP_666763.1 629145 D 393115 CDS YP_666764.1 110670207 4200611 complement(630200..631063) 1 NC_008245.1 Similar to Q9L6I3 Extended-spectrum beta-lactamase BES-1 from Serratia marcescens (292 aa).FASTA: opt: 621 Z-score: 723.1 E(): 2.2e-32 Smith-Waterman score: 621; 34.397 identity in 282 aa overlap ORF ftt0611c; beta-lactamase 631063 4200611 FTF0611c Francisella tularensis subsp. tularensis FSC198 beta-lactamase YP_666764.1 630200 R 393115 CDS YP_666765.1 110670208 4200612 631499..632311 1 NC_008245.1 ORF ftt0612; hypothetical protein 632311 4200612 FTF0612 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666765.1 631499 D 393115 CDS YP_666766.1 110670209 4200613 complement(632632..633051) 1 NC_008245.1 This ORF is longer. ORF ftt0613c; hypothetical protein 633051 4200613 FTF0613c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666766.1 632632 R 393115 CDS YP_666767.1 110670210 4200614 complement(633297..634787) 1 NC_008245.1 Similar to Q820B4 Apolipoprotein N-acyltransferase from Coxiella burnetii (485 aa). FASTA: opt: 984 Z-score: 1082.0 E(): 2.2e-52 Smith-Waterman score: 984; 35.903 identity in 493 aa overlap ORF ftt0614c; apolipoprotein N-acyltransferase 634787 4200614 FTF0614c Francisella tularensis subsp. tularensis FSC198 apolipoprotein N-acyltransferase YP_666767.1 633297 R 393115 CDS YP_666768.1 110670211 4200615 complement(634787..635647) 1 NC_008245.1 Similar to Q8EHP0 Magnesium and cobalt efflux protein CorC from Shewanella oneidensis (291 aa). FASTA: opt: 676 Z-score: 772.4 E(): 3.9e-35 Smith-Waterman score: 676; 39.847 identity in 261 aa overlap ORF ftt0615c; metal ion transporter protein 635647 4200615 FTF0615c Francisella tularensis subsp. tularensis FSC198 metal ion transporter protein YP_666768.1 634787 R 393115 CDS YP_666769.1 110670212 4200616 complement(635619..636125) 1 NC_008245.1 Similar to Q8EHN9 Hypothetical UPF0054 protein SO117 from Shewanella oneidensis (153 aa). FATSA: opt: 433 Z-score: 547.4 E(): 1.3e-22 Smith-Waterman score: 433; 44.025identity in 159 aa overlap ORF ftt0616c; hypothetical protein 636125 4200616 FTF0616c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666769.1 635619 R 393115 CDS YP_666770.1 110670213 4199727 complement(636100..637083) 1 NC_008245.1 Simliar to Q87VY0 PhoH-like protein from Pseudomonas syringae (340 aa). FASTA: opt: 1132 Z-score: 1306.4 E(): 7.1e-65 Smith-Waterman score: 1132; 52.923 identity in 325 aa overlap; phoH-like protein 637083 phoH 4199727 phoH Francisella tularensis subsp. tularensis FSC198 phoH-like protein YP_666770.1 636100 R 393115 CDS YP_666771.1 110670214 4199033 complement(637102..638430) 1 NC_008245.1 catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A); (dimethylallyl)adenosine tRNA methylthiotransferase 638430 yleA 4199033 yleA Francisella tularensis subsp. tularensis FSC198 (dimethylallyl)adenosine tRNA methylthiotransferase YP_666771.1 637102 R 393115 CDS YP_666772.1 110670215 4200617 638653..639327 1 NC_008245.1 Similar to O67476 O-methyltransferase from Aquifex aeolicus (212 aa). FASTA: opt: 382 Z-score: 477.6 E(): 1e-18 Smith-Waterman score: 382; 33.495 identity in 206 aa overlap. ORF ftt0619; o-methyltransferase family protein 639327 4200617 FTF0619 Francisella tularensis subsp. tularensis FSC198 o-methyltransferase family protein YP_666772.1 638653 D 393115 CDS YP_666773.1 110670216 4200618 639336..640079 1 NC_008245.1 Similar to Q8PER3 Acid phosphatase from Xanthomonas axonopodis (310 aa). FASTA: opt: 238 Z-score: 293.2 E(): 1.9e-08 Smith-Waterman score: 238; 24.583 identity in 240 aa overlap. ORF ftt0620; HAD superfamily protein 640079 4200618 FTF0620 Francisella tularensis subsp. tularensis FSC198 HAD superfamily protein YP_666773.1 639336 D 393115 CDS YP_666774.1 110670217 4199136 640095..640688 1 NC_008245.1 catalyzes the formation of thymidine 5'-phosphate from thymidine; thymidine kinase 640688 tdk 4199136 tdk Francisella tularensis subsp. tularensis FSC198 thymidine kinase YP_666774.1 640095 D 393115 CDS YP_666775.1 110670218 4200619 complement(640731..641195) 1 NC_008245.1 ORF ftt0622c; hypothetical protein 641195 4200619 FTF0622c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666775.1 640731 R 393115 CDS YP_666776.1 110670219 4199145 641350..642666 1 NC_008245.1 Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer; trigger factor 642666 tig 4199145 tig Francisella tularensis subsp. tularensis FSC198 trigger factor YP_666776.1 641350 D 393115 CDS YP_666777.1 110670220 4200267 642692..643297 1 NC_008245.1 hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates; ATP-dependent Clp protease proteolytic subunit 643297 clpP 4200267 clpP Francisella tularensis subsp. tularensis FSC198 ATP-dependent Clp protease proteolytic subunit YP_666777.1 642692 D 393115 CDS YP_666778.1 110670221 4200268 643321..644574 1 NC_008245.1 binds and unfolds substrates as part of the ClpXP protease; ATP-dependent protease ATP-binding subunit ClpX 644574 clpX 4200268 clpX Francisella tularensis subsp. tularensis FSC198 ATP-dependent protease ATP-binding subunit ClpX YP_666778.1 643321 D 393115 CDS YP_666779.1 110670222 4199562 644599..646923 1 NC_008245.1 Similar to Q8Z8V0 Lon protease from Salmonella typhi (784 aa). FASTA: opt: 2734 Z-score: 2797.3 E(): 6.4e-148 Smith-Waterman score: 2734; 54.450 identity in 764 aa overlap.; DNA-binding, ATP-dependent protease La 646923 lon 4199562 lon Francisella tularensis subsp. tularensis FSC198 DNA-binding, ATP-dependent protease La YP_666779.1 644599 D 393115 CDS YP_666780.1 110670223 4200650 647010..647282 1 NC_008245.1 Similar to Q93TM3 Histone-like protein HU form B from Pseudomonas putida (strain KT2440) (90 aa). FASTA: opt: 380 Z-score: 519.0 E(): 5.2e-21 Smith-Waterman score: 380; 68.539 identity in 89 aa overlap.; histone-like protein HU form B 647282 hupB 4200650 hupB Francisella tularensis subsp. tularensis FSC198 histone-like protein HU form B YP_666780.1 647010 D 393115 CDS YP_666781.1 110670224 4200620 647394..648824 1 NC_008245.1 Similar to PPID_BUCAP (Q8K987) Peptidyl-prolyl cis-trans isomerase from Buchnera aphidicola (subsp. Schizaphis graminum) (621 aa). FASTA: opt: 298 Z-score: 309.1E(): 2.5e-09 Smith-Waterman score: 298; 23.362 identity in 351 aa overlap. 200 aa shorter (in C-term) than most of the homologs. ORF ftt0628; hypothetical protein 648824 4200620 FTF0628 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666781.1 647394 D 393115 CDS YP_666782.1 110670225 4199844 648825..649751 1 NC_008245.1 IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity; tRNA delta(2)-isopentenylpyrophosphate transferase 649751 miaA 4199844 miaA Francisella tularensis subsp. tularensis FSC198 tRNA delta(2)-isopentenylpyrophosphate transferase YP_666782.1 648825 D 393115 CDS YP_666783.1 110670226 4200193 649862..650191 1 NC_008245.1 Similar to HFQ_PASMU (Q9CMC6) Hfq protein from Pasteurella multocida (95 aa). FASTA: opt: 384 Z-score: 504.4 E(): 3.3e-20 Smith-Waterman score: 384; 68.182 identity in 88 aa overlap. Plays a role in degradation of RNA transcripts.; host factor I for bacteriophage Q beta replication 650191 hfq 4200193 hfq Francisella tularensis subsp. tularensis FSC198 host factor I for bacteriophage Q beta replication YP_666783.1 649862 D 393115 CDS YP_666784.1 110670227 4200192 650243..651550 1 NC_008245.1 Similar to Q87VJ5 GTP-binding protein HflX from Pseudomonas syringae (pv. tomato) (433 aa). FASTA: opt: 1331 Z-score: 1520.3 E(): 8.6e-77 Smith-Waterman score: 1331; 49.302 identity in 430 aa overlap. Together with HflC-HflK involved in stability of phage lambda cII repressor; protease, GTP-binding subunit 651550 hflX 4200192 hflX Francisella tularensis subsp. tularensis FSC198 protease, GTP-binding subunit YP_666784.1 650243 D 393115 CDS YP_666785.1 110670228 4200621 complement(651561..652751) 1 NC_008245.1 Similar to O53772 Putative oxidoreductase (Monooxygenase) from Mycobacterium tuberculosis (388 aa). FASTA: opt: 832 Z-score: 997.9 E(): 1.1e-47 Smith-Waterman score: 832; 35.550 identity in 391 aa overlap. ORF ftt0632c; hypothetical protein 652751 4200621 FTF0632c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666785.1 651561 R 393115 CDS YP_666786.1 110670229 4200382 652923..653990 1 NC_008245.1 Similar to Q8FAK7 HflK protein from Escherichia coli (419 aa). FASTA: opt: 843 Z-score: 932.9 E(): 4.5e-44 Smith-Waterman score: 843; 40.0 identity in 330 aa overlap. With HflC, part of modulator for protease specific for FtsH phage lambda cII repressor.; hypothetical protein 653990 hflK 4200382 hflK Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666786.1 652923 D 393115 CDS YP_666787.1 110670230 4200381 653992..654918 1 NC_008245.1 Similar to Q8EJ66 HflC protein from Shewanella oneidensis (297 aa). FASTA: opt: 799 Z-score: 895.3 E(): 5.6e-42 Smith-Waterman score: 799; 42.466 identity in 292 aa overlap. With HflK, part of modulator for protease specific for FtsH phage lambda cII repressor.; hypothetical protein 654918 hflC 4200381 hflC Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666787.1 653992 D 393115 CDS YP_666788.1 110670231 4200689 654919..655404 1 NC_008245.1 Similar to O35016 YFKJ protein from Bacillus subtilis (156 aa). FASTA: opt: 391 Z-score: 494.5 E(): 1.2e-19 Smith-Waterman score: 391; 38.608 identity in 158 aa overlap.; low molecular weight (LMW) phosphotyrosine protein phosphatase 655404 wzb 4200689 wzb Francisella tularensis subsp. tularensis FSC198 low molecular weight (LMW) phosphotyrosine protein phosphatase YP_666788.1 654919 D 393115 CDS YP_666789.1 110670232 4199676 655401..655994 1 NC_008245.1 binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential; ribosome biogenesis GTP-binding protein YsxC 655994 engB 4199676 engB Francisella tularensis subsp. tularensis FSC198 ribosome biogenesis GTP-binding protein YsxC YP_666789.1 655401 D 393115 CDS YP_666790.1 110670233 4200622 657431..657781 1 NC_008245.1 Putative fragment. Weak similarity to leuA LE12_METJA (Q58595) 2-isopropylmalate synthase 2 from Methanococcus jannaschii (518 aa). FASTA: opt: 119 Z-score: 151.8 E(): 1.5 Smith-Waterman score: 119; 26.316 identity in 114 aa overlap (6-115:356-467). Next downstream ORF also has similarity to leuA. ORF ftt0638; hypothetical protein 657781 4200622 FTF0638 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666790.1 657431 D 393115 CDS YP_666791.1 110670234 4200623 657823..657933 1 NC_008245.1 Putative fragment. Weak similarity to leuA LE12_METJA (Q58595) 2-isopropylmalate synthase 2 from Methanococcus jannaschii (518 aa). FASTA: opt: 86 Z-score: 142.0 E(): 5.1 Smith-Waterman score: 86; 50.000 identity in 32 aa overlap (1-32:479-510). Next upstream ORF also have similarity to leuA. ORF ftt0639; hypothetical protein 657933 4200623 FTF0639 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666791.1 657823 D 393115 CDS YP_666792.1 110670235 4200664 657957..659612 1 NC_008245.1 catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis; dihydroxy-acid dehydratase 659612 ilvD 4200664 ilvD Francisella tularensis subsp. tularensis FSC198 dihydroxy-acid dehydratase YP_666792.1 657957 D 393115 CDS YP_666793.1 110670236 4200666 661475..661792 1 NC_008245.1 Similar to Q8A610 Acetohydroxyacid synthase small subunit (187 aa). FASTA: opt: 159 Z-score: 221.3 E(): 0.0002 Smith-Waterman score: 159; 33.333 identity in 75 aa overlap.; acetolactate synthase small subunit 661792 ilvH 4200666 ilvH Francisella tularensis subsp. tularensis FSC198 acetolactate synthase small subunit YP_666793.1 661475 D 393115 CDS YP_666794.1 110670237 4199841 complement(662987..666412) 1 NC_008245.1 Similar to Q9CM06 Mfd from Pasteurella multocida (1145 aa). FASTA: opt: 3312 Z-score: 3697.7 E(): 4.5e-198 Smith-Waterman score: 3312; 44.794 identity in 1143 aa overlap.; transcription-repair coupling factor 666412 mfd 4199841 mfd Francisella tularensis subsp. tularensis FSC198 transcription-repair coupling factor YP_666794.1 662987 R 393115 CDS YP_666795.1 110670238 4200624 complement(666457..667143) 1 NC_008245.1 Similar to YCCA_ECOLI (P06967) Hypothetical protein yccA from Escherichia coli (219 aa). FASTA: opt: 588 Z-score: 603.2 E(): 1.1e-25 Smith-Waterman score: 588; 48.416 identity in 221 aa overlap. ORF ftt0645c; hypothetical protein 667143 4200624 FTF0645c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666795.1 666457 R 393115 CDS YP_666796.1 110670239 4200625 complement(667259..668518) 1 NC_008245.1 ORF ftt0646c; hypothetical protein 668518 4200625 FTF0646c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666796.1 667259 R 393115 CDS YP_666797.1 110670240 4200626 complement(668518..668949) 1 NC_008245.1 Similar to Q82WE7 Hypothetical protein from Nitrosomonas europaea (164 aa). FASTA: opt: 383 Z-score: 490.4 E(): 2e-19 Smith-Waterman score: 383; 41.606 identity in 137 aa overlap. ORF ftt0647c; hypothetical protein 668949 4200626 FTF0647c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666797.1 668518 R 393115 CDS YP_666798.1 110670241 4200243 complement(668946..669584) 1 NC_008245.1 Similar to Q9HYB4 Endonuclease III from Pseudomonas aeruginosa (212 aa). FASTA: opt: 916 Z-score: 1153.9 E(): 2.2e-56 Smith-Waterman score: 916; 67.308identity in 208 aa overlap.; endonuclease III 669584 nth 4200243 nth Francisella tularensis subsp. tularensis FSC198 endonuclease III YP_666798.1 668946 R 393115 CDS YP_666799.1 110670242 4200764 complement(669577..670206) 1 NC_008245.1 Similar to Q83B23 4Fe-4S binding domain protein from Coxiella burnetii (213 aa). FASTA: opt: 552 Z-score: 680.7 E(): 5e-30 Smith-Waterman score: 552; 42.788 identity in 208 aa overlap.; Iron-sulfur cluster-binding protein 670206 rnfB 4200764 rnfB Francisella tularensis subsp. tularensis FSC198 Iron-sulfur cluster-binding protein YP_666799.1 669577 R 393115 CDS YP_666800.1 110670243 4200302 complement(670208..670852) 1 NC_008245.1 cofactor involved in the reduction of disulfides; glutaredoxin 670852 grxB 4200302 grxB Francisella tularensis subsp. tularensis FSC198 glutaredoxin YP_666800.1 670208 R 393115 CDS YP_666801.1 110670244 4200627 670914..672482 1 NC_008245.1 Similar to Q9L9P6 IraB from Legionella pneumophila (501 aa). FASTA: opt: 1486 Z-score: 1640.1 E(): 1.8e-83 Smith-Waterman score: 1486; 46.708 identity in 486 aa overlap. ORF ftt0651; proton-dependent oligopeptide transport (POT) family protein 672482 4200627 FTF0651 Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_666801.1 670914 D 393115 CDS YP_666802.1 110670245 4199191 complement(672485..672982) 1 NC_008245.1 Similar to Q899N4 Ferritin from Clostridium tetani (169 aa). FASTA: opt: 527 Z-score: 679.5 E(): 5.9e-30 Smith-Waterman score: 527; 45.783identity in 166 aa overlap.; Ferritin-like protein 672982 ftnA 4199191 ftnA Francisella tularensis subsp. tularensis FSC198 Ferritin-like protein YP_666802.1 672485 R 393115 CDS YP_666803.1 110670246 4199554 673189..674172 1 NC_008245.1 catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group; lipoyl synthase 674172 lipA 4199554 lipA Francisella tularensis subsp. tularensis FSC198 lipoyl synthase YP_666803.1 673189 D 393115 CDS YP_666804.1 110670247 4199671 674202..674861 1 NC_008245.1 Similar to Q87IN9 Sigma cross-reacting protein 27A from Vibrio parahaemolyticus (216 aa). FASTA: opt: 683 Z-score: 873.4 E(): 9.3e-41 Smith-Waterman score: 683; 49.074 identity in 216 aa overlap.; isoprenoid biosynthesis protein with amidotransferase-like domain 674861 elbB 4199671 elbB Francisella tularensis subsp. tularensis FSC198 isoprenoid biosynthesis protein with amidotransferase-like domain YP_666804.1 674202 D 393115 CDS YP_666805.1 110670248 4200628 674877..675623 1 NC_008245.1 Similar to YO32_SHEON (Q8EEF0) Hypothetical UPF0082 protein SO2432 from Shewanella oneidensis (248 aa). FASTA: opt: 1090 Z-score: 1308.8 E(): 5.2e-65 Smith-Waterman score: 1090; 64.516 identity in 248 aa overlap. ORF ftt0655; hypothetical protein 675623 4200628 FTF0655 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666805.1 674877 D 393115 CDS YP_666806.1 110670249 4198967 675699..676235 1 NC_008245.1 endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity; Holliday junction resolvase 676235 ruvC 4198967 ruvC Francisella tularensis subsp. tularensis FSC198 Holliday junction resolvase YP_666806.1 675699 D 393115 CDS YP_666807.1 110670250 4198965 677520..678173 1 NC_008245.1 plays an essential role in ATP-dependent branch migration of the Holliday junction; Holliday junction DNA helicase RuvA 678173 ruvA 4198965 ruvA Francisella tularensis subsp. tularensis FSC198 Holliday junction DNA helicase RuvA YP_666807.1 677520 D 393115 CDS YP_666808.1 110670251 4199479 678199..679611 1 NC_008245.1 Similar to Q87TH5 Conserved hypothetical protein from Vibrio parahaemolyticus (514 aa). FASTA: opt: 1129 Z-score: 1083.4 E(): 1.9e-52 Smith-Waterman score: 1132; 42.000 identity in 450 aa overlap ORF ftt0659; DNA recombination protein RmuC family protein 679611 4199479 FTF0659 Francisella tularensis subsp. tularensis FSC198 DNA recombination protein RmuC family protein YP_666808.1 678199 D 393115 CDS YP_666809.1 110670252 4199480 679660..680247 1 NC_008245.1 ORF ftt0660; hypothetical protein 680247 4199480 FTF0660 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666809.1 679660 D 393115 CDS YP_666810.1 110670253 4199481 complement(680269..680565) 1 NC_008245.1 Similar to Q82LF3 Hypothetical protein from Streptomyces avermitilis (109 aa). FASTA: opt: 260 Z-score: 364.1 E(): 2.2e-12 Smith-Waterman score: 260; 47.778 identity in 90 aa overlap. The homologs are of different sizes. ORF ftt0661c; hypothetical protein 680565 4199481 FTF0661c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666810.1 680269 R 393115 CDS YP_666811.1 110670254 4199482 complement(680728..681111) 1 NC_008245.1 Similar to Q9KMT3 Hypothetical protein VCA0237 from Vibrio cholerae (189 aa). FASTA: opt: 308 Z-score: 400.0 E(): 2.2e-14 Smith-Waterman score: 308; 42.857 identity in 112 aa overlap. ORF ftt0662c; hypothetical protein 681111 4199482 FTF0662c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666811.1 680728 R 393115 CDS YP_666812.1 110670255 4199483 681224..682036 1 NC_008245.1 ORF ftt0663; hypothetical protein 682036 4199483 FTF0663 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666812.1 681224 D 393115 CDS YP_666813.1 110670256 4200369 complement(682023..683159) 1 NC_008245.1 catalyzes the formation of histamine from L-histidine; histidine decarboxylase 683159 hdc 4200369 hdc Francisella tularensis subsp. tularensis FSC198 histidine decarboxylase YP_666813.1 682023 R 393115 CDS YP_666814.1 110670257 4199484 complement(683267..683872) 1 NC_008245.1 Similar to Q88IX8 Class II aldolase/adducin domain protein from Pseudomonas putida (strain KT2440) (251 aa). FASTA: opt: 472 Z-score: 599.3 E(): 1.7e-25 Smith-Waterman score: 472; 38.579identity in 197 aa overlap. ORF ftt0665c; aldolase/adducin class II family protein 683872 4199484 FTF0665c Francisella tularensis subsp. tularensis FSC198 aldolase/adducin class II family protein YP_666814.1 683267 R 393115 CDS YP_666815.1 110670258 4199485 complement(683963..684544) 1 NC_008245.1 Similar to Q896H4 DNA-3-methyladenine glycosylase from Clostridium tetani (203 aa). FASTA: opt: 451 Z-score: 589.0 E(): 6.4e-25 Smith-Waterman score: 451; 42.784 identity in 194 aa overlap. ORF ftt0666c; methylpurine-DNA glycosylase family protein 684544 4199485 FTF0666c Francisella tularensis subsp. tularensis FSC198 methylpurine-DNA glycosylase family protein YP_666815.1 683963 R 393115 CDS YP_666816.1 110670259 4199486 684672..685175 1 NC_008245.1 ORF ftt0667; hypothetical protein 685175 4199486 FTF0667 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666816.1 684672 D 393115 CDS YP_666817.1 110670260 4199487 685175..685807 1 NC_008245.1 Similar to Q8A546 Putative membrane transporter involved in nicotinamide mononucleotide transport from Bacteroides thetaiotaomicron (188 aa). FASTA: opt: 333 Z-score: 403.8 E(): 1.3e-14 Smith-Waterman score: 333; 29.016identity in 193 aa overlap. ORF ftt0668; nicotinamide mononucleotide transport (NMT) family protein 685807 4199487 FTF0668 Francisella tularensis subsp. tularensis FSC198 nicotinamide mononucleotide transport (NMT) family protein YP_666817.1 685175 D 393115 CDS YP_666818.1 110670261 4199488 685905..687080 1 NC_008245.1 Similar to Q8GRF6 MagA protein from Magnetospirillum magnetotacticum (Aquaspirillum magnetotacticum) (432 aa). FASTA: opt: 769 Z-score: 826.0 E(): 4.1e-38 Smith-Waterman score: 769; 31.635identity in 373 aa overlap. ORF ftt0669; Sodium/hydrogen exchanger family protein 687080 4199488 FTF0669 Francisella tularensis subsp. tularensis FSC198 Sodium/hydrogen exchanger family protein YP_666818.1 685905 D 393115 CDS YP_666819.1 110670262 4199489 complement(687413..687877) 1 NC_008245.1 ORF ftt0670c; hypothetical protein 687877 4199489 FTF0670c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666819.1 687413 R 393115 CDS YP_666820.1 110670263 4199490 688190..689470 1 NC_008245.1 Similar to Q83DX6 Major facilitator family transporter from Coxiella burnetii (421 aa). FASTA: opt: 689 Z-score: 731.9 E(): 7.1e-33 Smith-Waterman score: 689; 29.197identity in 411 aa overlap. ORF ftt0671; major facilitator transporter 689470 4199490 FTF0671 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666820.1 688190 D 393115 CDS YP_666821.1 110670264 4199492 complement(690742..691749) 1 NC_008245.1 Similar to Q8DF84 Predicted deacylase from Vibrio vulnificus (352 aa). FASTA: opt: 789 Z-score: 951.5 E(): 4.2e-45 Smith-Waterman score: 789; 34.328 identity in 335 aa overlap. ORF ftt0673c; hypothetical protein 691749 4199492 FTF0673c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666821.1 690742 R 393115 CDS YP_666822.1 110670265 4199945 691960..692928 1 NC_008245.1 Similar to KPRS_RALSO (Q8Y2E1) Ribose-phosphate pyrophosphokinase from Ralstonia solanacearum (Pseudomonas solanacearum) (316 aa). FASTA: opt: 1269 Z-score: 1520.3 E(): 8.6e-77 Smith-Waterman score: 1269; 58.413 identity in 315 aa overlap.; ribose-phosphate pyrophosphokinase 692928 prsA 4199945 prsA Francisella tularensis subsp. tularensis FSC198 ribose-phosphate pyrophosphokinase YP_666822.1 691960 D 393115 CDS YP_666823.1 110670266 4199001 693028..693318 1 NC_008245.1 the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response; 50S ribosomal protein L25 693318 rplY 4199001 rplY Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L25 YP_666823.1 693028 D 393115 CDS YP_666824.1 110670267 4199493 693414..694673 1 NC_008245.1 Similar to Q83E77 Hypothetical protein from Coxiella burnetii (417 aa). FASTA: opt: 1049 Z-score: 1211.5 E(): 1.4e-59 Smith-Waterman score: 1049; 40.049 identity in 407 aa overlap. ORF ftt0676; hypothetical protein 694673 4199493 FTF0676 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666824.1 693414 D 393115 CDS YP_666825.1 110670268 4199494 complement(694878..695654) 1 NC_008245.1 Similar to Q8Z984 Putative methyltransferase from Salmonella typhi (256 aa). FASTA: opt: 707 Z-score: 818.1 E(): 1.1e-37 Smith-Waterman score: 707; 42.570 identity in 249 aa overlap. ORF ftt0677c; hypothetical protein 695654 4199494 FTF0677c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666825.1 694878 R 393115 CDS YP_666826.1 110670269 4199495 complement(696263..696538) 1 NC_008245.1 ORF ftt0678c; hypothetical protein 696538 4199495 FTF0678c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666826.1 696263 R 393115 CDS YP_666827.1 110670270 4199496 complement(696553..697644) 1 NC_008245.1 translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1; GTP-dependent nucleic acid-binding protein EngD 697644 4199496 FTF0679c Francisella tularensis subsp. tularensis FSC198 GTP-dependent nucleic acid-binding protein EngD YP_666827.1 696553 R 393115 CDS YP_666828.1 110670271 4199948 complement(697663..698238) 1 NC_008245.1 Enables the recycling of peptidyl-tRNAs produced at termination of translation; peptidyl-tRNA hydrolase 698238 pth 4199948 pth Francisella tularensis subsp. tularensis FSC198 peptidyl-tRNA hydrolase YP_666828.1 697663 R 393115 CDS YP_666829.1 110670272 4200310 complement(698231..699115) 1 NC_008245.1 Similar to BLAC_YEREN (Q01166) Beta-lactamase precursor from Yersinia enterocolitica (294 aa). FASTA: opt: 670 Z-score: 829.7 E(): 2.5e-38 Smith-Waterman score: 670; 36.268 identity in 284 aa overlap.; Beta-lactamase class A 699115 blaA 4200310 blaA Francisella tularensis subsp. tularensis FSC198 Beta-lactamase class A YP_666829.1 698231 R 393115 CDS YP_666830.1 110670273 4199497 complement(699124..699969) 1 NC_008245.1 ORF ftt0682c; hypothetical protein 699969 4199497 FTF0682c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666830.1 699124 R 393115 CDS YP_666831.1 110670274 4199731 complement(700009..700827) 1 NC_008245.1 Similar to Q888U2 Type IV pilus prepilin peptidase PilD from Pseudomonas syringae (pv. tomato) (290 aa). FASTA: opt: 784 Z-score: 898.0 E(): 4e-42 Smith-Waterman score: 784; 44.531identity in 256 aa overlap. Cleaves the leader peptide of the fiber building block proteins and methylates the resulting N-terminal residue.; Type IV pili leader peptidase and methylase 700827 pilD 4199731 pilD Francisella tularensis subsp. tularensis FSC198 Type IV pili leader peptidase and methylase YP_666831.1 700009 R 393115 CDS YP_666832.1 110670275 4199086 complement(700831..702246) 1 NC_008245.1 catalyzes the conversion of NADPH to NADH; soluble pyridine nucleotide transhydrogenase 702246 sthA 4199086 sthA Francisella tularensis subsp. tularensis FSC198 soluble pyridine nucleotide transhydrogenase YP_666832.1 700831 R 393115 CDS YP_666833.1 110670276 4199036 complement(702500..703483) 1 NC_008245.1 Similar to KCH_ECOLI (P31069) Putative potassium channel protein (417 aa). FASTA: opt: 381 Z-score: 423.3 E(): 1.1e-15 Smith-Waterman score: 381; 31.203identity in 266 aa overlap. ORF ftt0685c; potassium channel protein 703483 4199036 FTF0685c Francisella tularensis subsp. tularensis FSC198 potassium channel protein YP_666833.1 702500 R 393115 CDS YP_666834.1 110670277 4199037 complement(703563..704867) 1 NC_008245.1 Similar to Q83E30 Proton/peptide symporter family protein from Coxiella burnetii (492 aa). FASTA: opt: 609 Z-score: 644.8 E(): 5e-28 Smith-Waterman score: 609; 26.545 identity in 437 aa overlap. The start is approx 55 aa later than the homologs. ORF ftt0686c; proton-dependent oligopeptide transport (POT) family protein 704867 4199037 FTF0686c Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_666834.1 703563 R 393115 CDS YP_666835.1 110670278 4200208 complement(705114..706481) 1 NC_008245.1 heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease ATP-binding subunit HslU 706481 hslU 4200208 hslU Francisella tularensis subsp. tularensis FSC198 ATP-dependent protease ATP-binding subunit HslU YP_666835.1 705114 R 393115 CDS YP_666836.1 110670279 4200209 complement(706489..707040) 1 NC_008245.1 heat shock protein involved in degradation of misfolded proteins; ATP-dependent protease peptidase subunit 707040 hslV 4200209 hslV Francisella tularensis subsp. tularensis FSC198 ATP-dependent protease peptidase subunit YP_666836.1 706489 R 393115 CDS YP_666837.1 110670280 4199038 707232..707981 1 NC_008245.1 ORF ftt0689; hypothetical protein 707981 4199038 FTF0689 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666837.1 707232 D 393115 CDS YP_666838.1 110670281 4199566 complement(707984..708886) 1 NC_008245.1 Similar to Q9KS71 Lipoate-protein ligase A from Vibrio cholerae (338 aa). FASTA: opt: 699 Z-score: 831.1 E(): 2.1e-38 Smith-Waterman score: 699; 38.545identity in 275 aa overlap.; lipoate-protein ligase A 708886 lplA 4199566 lplA Francisella tularensis subsp. tularensis FSC198 lipoate-protein ligase A YP_666838.1 707984 R 393115 CDS YP_666839.1 110670282 4198975 708999..710189 1 NC_008245.1 catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr); tyrosyl-tRNA synthetase 710189 tyrS 4198975 tyrS Francisella tularensis subsp. tularensis FSC198 tyrosyl-tRNA synthetase YP_666839.1 708999 D 393115 CDS YP_666840.1 110670283 4199632 complement(join(710690..711148,711148..711474)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 711474 isftu1 4199632 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666840.1 710690 R 393115 CDS YP_666841.1 110670284 4200113 complement(711545..712369) 1 NC_008245.1 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases; formamidopyrimidine-DNA glycosylase 712369 mutM 4200113 mutM Francisella tularensis subsp. tularensis FSC198 formamidopyrimidine-DNA glycosylase YP_666841.1 711545 R 393115 CDS YP_666842.1 110670285 4199039 712463..714562 1 NC_008245.1 Similar to AAP18158 2-acyl-glycerophospho-ethanolamine acyltransferase,acyl-acyl-carrier protein synthetase from Shigella flexneri (719 aa). FASTA: opt: 735 Z-score: 862.5 E(): 3.8e-40 Smith-Waterman score: 826; 33.661identity in 508 aa overlap. n. b. No match between aa 1 and 196. Several domains missing compared to AAP18158 ORF ftt0694; hypothetical protein 714562 4199039 FTF0694 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666842.1 712463 D 393115 CDS YP_666843.1 110670286 4199852 714568..715485 1 NC_008245.1 Similar to AAP95225 S-adenosyl-methyltransferase MraW from Haemophilus ducreyi (313 aa). FASTA: opt: 918 Z-score: 1107.1 E(): 8.9e-54 Smith-Waterman score: 918; 49.677identity in 310 aa overlap.; S-adenosyl-methyltransferase MraW 715485 mraW 4199852 mraW Francisella tularensis subsp. tularensis FSC198 S-adenosyl-methyltransferase MraW YP_666843.1 714568 D 393115 CDS YP_666844.1 110670287 4199040 715482..715832 1 NC_008245.1 Weak similarity to cell division protein ftsL. ORF ftt0696; hypothetical protein 715832 4199040 FTF0696 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666844.1 715482 D 393115 CDS YP_666845.1 110670288 4199194 715825..717519 1 NC_008245.1 Similar to Q83F33 Penicillin-binding protein 3 from Coxiella burnetii (548 aa). FASTA: opt: 1361 Z-score: 1597.9 E(): 4.1e-81 Smith-Waterman score: 1361; 40.325 identity in 553 aa overlap.; penicillin binding protein (peptidoglycan synthetase) 717519 ftsI 4199194 ftsI Francisella tularensis subsp. tularensis FSC198 penicillin binding protein (peptidoglycan synthetase) YP_666845.1 715825 D 393115 CDS YP_666846.1 110670289 4198953 717625..717891 1 NC_008245.1 primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence; 30S ribosomal protein S15 717891 rpsO 4198953 rpsO Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S15 YP_666846.1 717625 D 393115 CDS YP_666847.1 110670290 4199735 717948..720029 1 NC_008245.1 Similar to Q83D87 Polyribonucleotide nucleotidyltransferase from Coxiella burnetii (696 aa). FASTA: opt: 2437 Z-score: 2800.2 E(): 4.4e-148 Smith-Waterman score: 2437; 54.401identity in 693 aa overlap.; polynucleotide phosphorylase/polyadenylase 720029 pnp 4199735 pnp Francisella tularensis subsp. tularensis FSC198 polynucleotide phosphorylase/polyadenylase YP_666847.1 717948 D 393115 CDS YP_666848.1 110670291 4199041 720035..720385 1 NC_008245.1 essential respiratory protein A; may be involved in the transfer of iron-sulfur clusters; essential for growth using oxygen or alternate electron acceptors; iron-sulfur cluster insertion protein ErpA 720385 4199041 FTF0700 Francisella tularensis subsp. tularensis FSC198 iron-sulfur cluster insertion protein ErpA YP_666848.1 720035 D 393115 CDS YP_666849.1 110670292 4199506 720395..721243 1 NC_008245.1 catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis; 2-dehydro-3-deoxyphosphooctonate aldolase 721243 kdsA 4199506 kdsA Francisella tularensis subsp. tularensis FSC198 2-dehydro-3-deoxyphosphooctonate aldolase YP_666849.1 720395 D 393115 CDS YP_666850.1 110670293 4198981 721254..721919 1 NC_008245.1 functions in pyrimidine salvage; pyrimidine ribonucleoside kinase; phosphorylates nucleosides or dinucleosides to make UMP or CMP using ATP or GTP as the donor; uridine kinase 721919 udk 4198981 udk Francisella tularensis subsp. tularensis FSC198 uridine kinase YP_666850.1 721254 D 393115 CDS YP_666851.1 110670294 4200332 721912..722130 1 NC_008245.1 Similar to RPOZ_PSEPK (Q88C82) Probable DNA-directed RNA polymerase omega chain from Pseudomonas putida (strain KT2440) (87 aa). FASTA: opt: 194 Z-score: 293.6 E(): 1.8e-08 Smith-Waterman score: 194; 52.857 identity in 70 aa overlap.; DNA-directed RNA polymerase subunit omega 722130 rpoZ 4200332 rpoZ Francisella tularensis subsp. tularensis FSC198 DNA-directed RNA polymerase subunit omega YP_666851.1 721912 D 393115 CDS YP_666852.1 110670295 4199042 722395..722967 1 NC_008245.1 ORF ftt0704; hypothetical protein 722967 4199042 FTF0704 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666852.1 722395 D 393115 CDS YP_666853.1 110670296 4198964 723056..724405 1 NC_008245.1 in Escherichia coli this enzyme catalyzes the SAM-dependent methylation of U1939 in the 23S ribomal RNA; binds an iron-sulfur cluster [4Fe-4S]; 23S rRNA 5-methyluridine methyltransferase 724405 rumA 4198964 rumA Francisella tularensis subsp. tularensis FSC198 23S rRNA 5-methyluridine methyltransferase YP_666853.1 723056 D 393115 CDS YP_666854.1 110670297 4199043 725336..726130 1 NC_008245.1 Similar to Q9CK00 Hypothetical protein PM1838 from Pasteurella multocida (234 aa). FASTA: opt: 456 Z-score: 507.2 E(): 2.3e-20 Smith-Waterman score: 456; 34.483identity in 232 aa overlap. ORF ftt0707; nicotinamide mononucleotide transport (NMT) family protein 726130 4199043 FTF0707 Francisella tularensis subsp. tularensis FSC198 nicotinamide mononucleotide transport (NMT) family protein YP_666854.1 725336 D 393115 CDS YP_666855.1 110670298 4199044 726218..727471 1 NC_008245.1 Similar to Q83BD8 Major facilitator family transporter from Coxiella burnetii (411 aa). FASTA: opt: 868 Z-score: 941.6 E(): 1.5e-44 Smith-Waterman score: 868; 37.912identity in 364 aa overlap. ORF ftt0708; major facilitator transporter 727471 4199044 FTF0708 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666855.1 726218 D 393115 CDS YP_666856.1 110670299 4199677 727580..728950 1 NC_008245.1 enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis; phosphopyruvate hydratase 728950 eno 4199677 eno Francisella tularensis subsp. tularensis FSC198 phosphopyruvate hydratase YP_666856.1 727580 D 393115 CDS YP_666857.1 110670300 4199193 728958..729248 1 NC_008245.1 Similar to FTSB_VIBPA (Q87LQ1) Cell division protein ftsB homolog from Vibrio parahaemolyticus (93 aa). FASTA: opt: 198 Z-score: 273.8 E(): 2.3e-07 Smith-Waterman score: 198; 37.209 identity in 86 aa overlap.; cell division protein 729248 ftsB 4199193 ftsB Francisella tularensis subsp. tularensis FSC198 cell division protein YP_666857.1 728958 D 393115 CDS YP_666858.1 110670301 4199300 729238..729927 1 NC_008245.1 Similar to ISPD_PASMU (P57953) 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (238 aa). FASTA: opt: 506 Z-score: 656.8 E(): 1.1e-28 Smith-Waterman score: 506; 39.381 identity in 226 aa overlap.; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 729927 ispD 4199300 ispD Francisella tularensis subsp. tularensis FSC198 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase YP_666858.1 729238 D 393115 CDS YP_666859.1 110670302 4200727 complement(729932..731194) 1 NC_008245.1 Similar to SDAC_HAEIN (P44615) Serine transporter from Haemophilus influenzae (412 aa). FASTA: opt: 785 Z-score: 854.1 E(): 1.1e-39 Smith-Waterman score: 785; 33.099 identity in 426 aa overlap.; serine transporter 731194 sdaC1 4200727 sdaC1 Francisella tularensis subsp. tularensis FSC198 serine transporter YP_666859.1 729932 R 393115 CDS YP_666860.1 110670303 4199135 complement(731196..732251) 1 NC_008245.1 converts threonine and NAD to 1,2-amino-3-oxobutanoate and NADH; functions in threonine catabolism; L-threonine 3-dehydrogenase 732251 tdh 4199135 tdh Francisella tularensis subsp. tularensis FSC198 L-threonine 3-dehydrogenase YP_666860.1 731196 R 393115 CDS YP_666861.1 110670304 4199500 complement(732274..733470) 1 NC_008245.1 catalyzes the formation of 2-amino-3-oxobutanoate from acetyl-CoA and glycine; 2-amino-3-ketobutyrate coenzyme A ligase 733470 kbl 4199500 kbl Francisella tularensis subsp. tularensis FSC198 2-amino-3-ketobutyrate coenzyme A ligase YP_666861.1 732274 R 393115 CDS YP_666862.1 110670305 4199045 734003..736285 1 NC_008245.1 Similar to Q9ZIX2 Chitinase c from Pseudoalteromonas sp. S9 (869 aa). FASTA: opt: 331 Z-score: 370.4 E(): 9.6e-13 Smith-Waterman score: 583; 24.111 identity in 900 aa overlap. The homologs are of varying sizes. ORF ftt0715; chitinase family 18 protein 736285 4199045 FTF0715 Francisella tularensis subsp. tularensis FSC198 chitinase family 18 protein YP_666862.1 734003 D 393115 CDS YP_666863.1 110670306 4198984 736464..737108 1 NC_008245.1 Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate; uracil phosphoribosyltransferase 737108 upp 4198984 upp Francisella tularensis subsp. tularensis FSC198 uracil phosphoribosyltransferase YP_666863.1 736464 D 393115 CDS YP_666864.1 110670307 4199046 738116..738460 1 NC_008245.1 ORF ftt0718; hypothetical protein 738460 4199046 FTF0718 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666864.1 738116 D 393115 CDS YP_666865.1 110670308 4199047 738517..739740 1 NC_008245.1 Aa 139-320 identical to AAP97846 Hypothetical protein (Fragment) from Francisella tularensis ssp. tularensis strain SchuS4 (184 aa). Similar to Q881T0 Proline/betaine transporter from Pseudomonas syringae (pv. tomato) (438 aa). FASTA: opt: 561 Z-score: 633.8 E(): 2.1e-27 Smith-Waterman score: 561; 25.616 identity in 406 aa overlap. ORF ftt0719; major facilitator transporter 739740 4199047 FTF0719 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666865.1 738517 D 393115 CDS YP_666866.1 110670309 4199348 complement(739724..741049) 1 NC_008245.1 Similar to DGT1_VIBPA (Q87R73) Deoxyguanosinetriphosphate triphosphohydrolase-like protein from Vibrio parahaemolyticus (440 aa). FASTA: opt: 587 Z-score: 683.8 E(): 3.4e-30 Smith-Waterman score: 661; 33.781identity in 447 aa overlap.; deoxyguanosinetriphosphate triphosphohydrolase 741049 dgt 4199348 dgt Francisella tularensis subsp. tularensis FSC198 deoxyguanosinetriphosphate triphosphohydrolase YP_666866.1 739724 R 393115 CDS YP_666867.1 110670310 4199499 complement(741153..743378) 1 NC_008245.1 Similar to CATA_YERPE (Q9X6B0) Peroxidase/catalase (EC 1.11.1.6) from Yersinia pestis (737 aa). FASTA: opt: 3041 Z-score: 3508.0 E(): 1.7e-187 Smith-Waterman score: 3041; 59.189 identity in 740 aa overlap.; peroxidase/catalase 743378 katG 4199499 katG Francisella tularensis subsp. tularensis FSC198 peroxidase/catalase YP_666867.1 741153 R 393115 CDS YP_666868.1 110670311 4199633 complement(join(744242..744700,744700..745026)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 745026 isftu1 4199633 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666868.1 744242 R 393115 CDS YP_666869.1 110670312 4199048 complement(745333..746121) 1 NC_008245.1 Similar to Q88H18 Oxidoreductase, short chain dehydrogenase/reductase family Pseudomonas putida (strain KT2440) (248 aa). FASTA: opt: 792 Z-score: 881.9 E(): 3.1e-41 Smith-Waterman score: 792; 47.984 identity in 248 aa overlap. ORF ftt0723c; short-chain dehydrogenase 746121 4199048 FTF0723c Francisella tularensis subsp. tularensis FSC198 short-chain dehydrogenase YP_666869.1 745333 R 393115 CDS YP_666870.1 110670313 4199798 complement(747524..748840) 1 NC_008245.1 Similar to Q81V41 Glycerol-3-phosphate transporter from Bacillus anthracis (strain Ames) (449 aa). FASTA: opt: 1382 Z-score: 1521.3 E(): 7.6e-77 Smith-Waterman score: 1382; 48.268identity in 433 aa overlap.; glycerol-3-phosphate transporter 748840 glpT 4199798 glpT Francisella tularensis subsp. tularensis FSC198 glycerol-3-phosphate transporter YP_666870.1 747524 R 393115 CDS YP_666871.1 110670314 4199049 complement(748947..749981) 1 NC_008245.1 Similar to Q89FW5 Blr6584 protein from Bradyrhizobium japonicum (342 aa). FASTA: opt: 636 Z-score: 743.1 E(): 1.7e-33 Smith-Waterman score: 743; 36.336identity in 333 aa overlap. ORF ftt0726c; glycerophosphoryl diester phosphodiesterase family protein 749981 4199049 FTF0726c Francisella tularensis subsp. tularensis FSC198 glycerophosphoryl diester phosphodiesterase family protein YP_666871.1 748947 R 393115 CDS YP_666872.1 110670315 4199050 750119..750799 1 NC_008245.1 Similar to Q83FA0 Lipoprotein, putative from Coxiella burnetii (326 aa). FASTA: opt: 255 Z-score: 290.7 E(): 2.7e-08 Smith-Waterman score: 255; 26.606 identity in 218 aa overlap. n. b. The ORF is 100 aa shorter than all homologs (C-term). ORF ftt0727; hypothetical protein 750799 4199050 FTF0727 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666872.1 750119 D 393115 CDS YP_666873.1 110670316 4200704 751072..752004 1 NC_008245.1 Similar to Q89II5 ABC transporter ATP-binding protein (310 aa). FASTA: opt: 1060 Z-score: 1223.8 E(): 2.8e-60 Smith-Waterman score: 1060; 54.605 identity in 304 aa overlap.; ABC transporter ATP-binding protein 752004 ybhF 4200704 ybhF Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_666873.1 751072 D 393115 CDS YP_666874.1 110670317 4200706 752001..753119 1 NC_008245.1 Similar to Q8Y316 Probable transmembrane protein from Ralstonia solanacearum (Pseudomonas solanacearum) (381 aa). FASTA: opt: 1138 Z-score: 1260.3 E(): 2.6e-62 Smith-Waterman score: 1138; 45.722 identity in 374 aa overlap.; ABC transporter membrane protein 753119 ybhR 4200706 ybhR Francisella tularensis subsp. tularensis FSC198 ABC transporter membrane protein YP_666874.1 752001 D 393115 CDS YP_666875.1 110670318 4199290 753217..753960 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 753960 isftu2 4199290 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666875.1 753217 D 393115 CDS YP_666876.1 110670319 4199051 complement(754200..754646) 1 NC_008245.1 ORF ftt0731c; hypothetical protein 754646 4199051 FTF0731c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666876.1 754200 R 393115 CDS YP_666877.1 110670320 4199052 754743..755090 1 NC_008245.1 ORF ftt0732; hypothetical protein 755090 4199052 FTF0732 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666877.1 754743 D 393115 CDS YP_666878.1 110670321 4199053 755119..755589 1 NC_008245.1 Similar to Q9A7J3 Glutathione peroxidase from Caulobacter crescentus (160 aa). FASTA: opt: 605 Z-score: 801.1 E(): 9.9e-37 Smith-Waterman score: 605; 55.696 identity in 158 aa overlap. ORF ftt0733; glutathione peroxidase 755589 4199053 FTF0733 Francisella tularensis subsp. tularensis FSC198 glutathione peroxidase YP_666878.1 755119 D 393115 CDS YP_666879.1 110670322 4199171 757877..758524 1 NC_008245.1 Similar to Q8REM4 Hypothetical protein FN1075 (215 aa). FASTA: opt: 221 Z-score: 288.5 E(): 3.5e-08 Smith-Waterman score: 221; 25.463identity in 216 aa overlap. ORF ftt0736; hypothetical protein 758524 4199171 FTF0736 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666879.1 757877 D 393115 CDS YP_666880.1 110670323 4199172 complement(758535..758663) 1 NC_008245.1 ORF ftt0737c; hypothetical protein 758663 4199172 FTF0737c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666880.1 758535 R 393115 CDS YP_666881.1 110670324 4199113 758831..760375 1 NC_008245.1 Similar to Q82Y67 Possible sugar kinase from Nitrosomonas europaea (505 aa). FASTA: Z-score: 922.3 E(): 1.8e-43 Smith-Waterman score: 856; 35.529 identity in 501 aa overlap.; carbohydrate kinase family protein (YjeF-related protein) 760375 yjeF 4199113 yjeF Francisella tularensis subsp. tularensis FSC198 carbohydrate kinase family protein (YjeF-related protein) YP_666881.1 758831 D 393115 CDS YP_666882.1 110670325 4200207 complement(760359..760727) 1 NC_008245.1 Similar to Q88AZ6 Heat shock protein 15 from Pseudomonas syringae (pv. tomato) (135 aa). FASTA: Z-score: 549.3 E(): 1.1e-22 Smith-Waterman score: 447; 58.824 identity in 119 aa overlap.; heat shock protein 15 (HSP15) 760727 hslR 4200207 hslR Francisella tularensis subsp. tularensis FSC198 heat shock protein 15 (HSP15) YP_666882.1 760359 R 393115 CDS YP_666883.1 110670326 4200088 complement(761131..763296) 1 NC_008245.1 Similar to OSTA_XYLFT (Q87AI9) Organic solvent tolerance protein from Xylella fastidiosa (strain Temecula1 / ATCC 700964) (792 aa). FASTA: opt: 653 Z-score: 763.8 E(): 1.2e-34 Smith-Waterman score: 678; 24.212 identity in 698 aa overlap.; organic solvent tolerance protein 763296 ostA2 4200088 ostA2 Francisella tularensis subsp. tularensis FSC198 organic solvent tolerance protein YP_666883.1 761131 R 393115 CDS YP_666884.1 110670327 4199173 complement(763318..763548) 1 NC_008245.1 ORF ftt0741c; hypothetical protein 763548 4199173 FTF0741c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666884.1 763318 R 393115 CDS YP_666885.1 110670328 4199174 763856..765844 1 NC_008245.1 ORF ftt0742; lipoprotein 765844 4199174 FTF0742 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666885.1 763856 D 393115 CDS YP_666886.1 110670329 4199175 766525..767340 1 NC_008245.1 Similar to Q87Q96 Conserved hypothetical protein from Vibrio parahaemolyticus (274 aa). FASTA: opt: 950 Z-score: 1132.9 E(): 3.3e-55 Smith-Waterman score: 950; 49.259 identity in 270 aa overlap. ORF ftt0743; hypothetical protein 767340 4199175 FTF0743 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666886.1 766525 D 393115 CDS YP_666887.1 110670330 4199176 complement(767439..767858) 1 NC_008245.1 ORF ftt0744c; hypothetical protein 767858 4199176 FTF0744c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666887.1 767439 R 393115 CDS YP_666888.1 110670331 4199177 complement(767880..768236) 1 NC_008245.1 ORF ftt0745c; hypothetical protein 768236 4199177 FTF0745c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666888.1 767880 R 393115 CDS YP_666889.1 110670332 4199178 complement(768334..769125) 1 NC_008245.1 Similar to Q88NQ5 Membrane protein, putative from Pseudomonas putida (strain KT2440) (662 aa). FASTA: opt: 267 Z-score: 319.4 E(): 6.8e-10 Smith-Waterman score: 269; 27.179 identity in 195 aa overlap. ORF ftt0746c; hypothetical protein 769125 4199178 FTF0746c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666889.1 768334 R 393115 CDS YP_666890.1 110670333 4199179 complement(769115..769900) 1 NC_008245.1 Similar to Q88NQ3 Membrane fusion protein,putative (289 aa). FASTA: opt: 721 Z-score: 853.4 E(): 1.2e-39 Smith-Waterman score: 721; 41.434identity in 251 aa overlap. Similar to ydhJ and yhcQ in E. coli. ORF ftt0747c; hypothetical protein 769900 4199179 FTF0747c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666890.1 769115 R 393115 CDS YP_666891.1 110670334 4199180 770280..770999 1 NC_008245.1 Similar to Q93PU6 Putative regulatory protein TtgV from Pseudomonas putida (259 aa). FASTA: opt: 485 Z-score: 588.0 E(): 7.4e-25 Smith-Waterman score: 485; 33.473 identity in 239 aa overlap. ORF ftt0748; hypothetical protein 770999 4199180 FTF0748 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666891.1 770280 D 393115 CDS YP_666892.1 110670335 4199181 complement(771059..772045) 1 NC_008245.1 Aa 8-183 identical to AAP97845 Hypothetical protein (Fragment) from Francisella tularensis ssp. tularensis strain SchuS4 (176 aa). Similar to Q8CL71 Hypothetical from Yersinia pestis (311 aa). FASTA: opt: 842 Z-score: 992.9 E(): 2e-47 Smith-Waterman score: 842; 44.710 identity in 293 aa overlap ORF ftt0749c; hypothetical protein 772045 4199181 FTF0749c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666892.1 771059 R 393115 CDS YP_666893.1 110670336 4199182 772251..773237 1 NC_008245.1 Similar to Q83AM1 Radical SAM domain protein from Coxiella burnetii (342 aa). FASTA: opt: 1094 Z-score: 1304.2 E(): 9.4e-65 Smith-Waterman score: 1094; 48.930 identity in 327 aa overlap. ORF ftt0750; hypothetical protein 773237 4199182 FTF0750 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666893.1 772251 D 393115 CDS YP_666894.1 110670337 4200279 complement(773308..773511) 1 NC_008245.1 Similar to Q89UH2 Cold shock protein from Bradyrhizobium japonicum (70 aa). FASTA: opt: 297 Z-score: 443.7 E(): 8e-17 Smith-Waterman score: 297; 66.176 identity in 68 aa overlap.; cold shock protein 773511 cspA 4200279 cspA Francisella tularensis subsp. tularensis FSC198 cold shock protein YP_666894.1 773308 R 393115 CDS YP_666895.1 110670338 4199183 complement(773710..773907) 1 NC_008245.1 ORF ftt0752c; hypothetical protein 773907 4199183 FTF0752c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666895.1 773710 R 393115 CDS YP_666896.1 110670339 4198960 774283..774483 1 NC_008245.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 774483 rpsU 4198960 rpsU Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S21 YP_666896.1 774283 D 393115 CDS YP_666897.1 110670340 4199184 complement(774541..774720) 1 NC_008245.1 ORF ftt0754c; hypothetical protein 774720 4199184 FTF0754c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666897.1 774541 R 393115 CDS YP_666898.1 110670341 4199185 775136..775630 1 NC_008245.1 ORF ftt0755; hypothetical protein 775630 4199185 FTF0755 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666898.1 775136 D 393115 CDS YP_666899.1 110670342 4199186 775721..776860 1 NC_008245.1 Similar to Q83BX5 Cation-efflux family protein from Coxiella burnetii (378 aa). FASTA: opt: 773 Z-score: 908.9 E(): 9.8e-43 Smith-Waterman score: 774; 34.605 identity in 367 aa overlap. ORF ftt0756; cation-efflux family protein 776860 4199186 FTF0756 Francisella tularensis subsp. tularensis FSC198 cation-efflux family protein YP_666899.1 775721 D 393115 CDS YP_666900.1 110670343 4199271 776877..777740 1 NC_008245.1 Similar to Q9K0G7 UTP--glucose-1-phosphate uridylyltransferase from Neisseria meningitidis (serogroup B) (289 aa). FASTA: opt: 1017 Z-score: 1291.1 E(): 5e-64 Smith-Waterman score: 1017; 57.439 identity in 289 aa overlap.; UTP--glucose-1-phosphate uridylyltransferase 777740 galU 4199271 galU Francisella tularensis subsp. tularensis FSC198 UTP--glucose-1-phosphate uridylyltransferase YP_666900.1 776877 D 393115 CDS YP_666901.1 110670344 4199187 779213..780130 1 NC_008245.1 Similar to YYAM_BACSU (P37511) Hypothetical transport protein yyaM from Bacillus subtilis (305 aa). FASTA: opt: 402 Z-score: 452.6 E(): 2.6e-17 Smith-Waterman score: 402; 25.614 identity in 285 aa overlap. ORF ftt0759; hypothetical protein 780130 4199187 FTF0759 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666901.1 779213 D 393115 CDS YP_666902.1 110670345 4199188 complement(780133..780339) 1 NC_008245.1 ORF ftt0760c; lipoprotein 780339 4199188 FTF0760c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666902.1 780133 R 393115 CDS YP_666903.1 110670346 4199189 complement(780424..780717) 1 NC_008245.1 ORF ftt0761c; hypothetical protein 780717 4199189 FTF0761c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666903.1 780424 R 393115 CDS YP_666904.1 110670347 4200603 complement(780739..781788) 1 NC_008245.1 Similar to RECF_PROMI (P22839) DNA replication and repair protein from Proteus mirabilis (362 aa). FASTA: opt: 874 Z-score: 1010.7 E(): 2.1e-48 Smith-Waterman score: 874; 37.394 identity in 353 aa overlap.; DNA replication and repair protein RecF 781788 recF 4200603 recF Francisella tularensis subsp. tularensis FSC198 DNA replication and repair protein RecF YP_666904.1 780739 R 393115 CDS YP_666905.1 110670348 4199032 complement(781792..782538) 1 NC_008245.1 Similar to YJJV_ECOLI (P39408) Putative deoxyribonuclease yjjV (259 aa). FASTA: opt: 503 Z-score: 595.3 E(): 2.9e-25 Smith-Waterman score: 503; 31.048 identity in 248 aa overlap.; TatD related DNAse family protein 782538 yjjV 4199032 yjjV Francisella tularensis subsp. tularensis FSC198 TatD related DNAse family protein YP_666905.1 781792 R 393115 CDS YP_666906.1 110670349 4200290 782731..784815 1 NC_008245.1 Similar to SYGB_COXBU (P45651) Glycyl-tRNA synthetase beta chain from Coxiella burnetii (689 aa). FASTA: opt: 1810 Z-score: 2108.8 E(): 1.4e-109 Smith-Waterman score: 1810; 42.939 identity in 694 aa overlap.; glycyl-tRNA synthetase subunit beta 784815 glyS 4200290 glyS Francisella tularensis subsp. tularensis FSC198 glycyl-tRNA synthetase subunit beta YP_666906.1 782731 D 393115 CDS YP_666907.1 110670350 4199634 join(784955..785281,785281..785739) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 785739 isftu1 4199634 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666907.1 784955 D 393115 CDS YP_666908.1 110670351 4199346 785908..786630 1 NC_008245.1 Similar to Q9PQL0 Purine-nucleoside phosphorylase from Ureaplasma parvum (Ureaplasma urealyticum biotype 1) (239 aa). FASTA: opt: 697 Z-score: 837.2 E(): 9.7e-39 Smith-Waterman score: 697; 47.534 identity in 223 aa overlap.; purine nucleoside phosphorylase 786630 deoD 4199346 deoD Francisella tularensis subsp. tularensis FSC198 purine nucleoside phosphorylase YP_666908.1 785908 D 393115 CDS YP_666909.1 110670352 4199912 complement(786627..787076) 1 NC_008245.1 ORF ftt0767c; hypothetical protein 787076 4199912 FTF0767c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666909.1 786627 R 393115 CDS YP_666910.1 110670353 4199913 complement(787080..788108) 1 NC_008245.1 ORF ftt0768c; hypothetical protein 788108 4199913 FTF0768c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666910.1 787080 R 393115 CDS YP_666911.1 110670354 4200733 788240..790960 1 NC_008245.1 functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins; preprotein translocase subunit SecA 790960 secA 4200733 secA Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecA YP_666911.1 788240 D 393115 CDS YP_666912.1 110670355 4198997 793029..793343 1 NC_008245.1 Similar to AAP95195 50S ribosomal protein L21 from Haemophilus ducreyi (103 aa). FASTA: opt: 435 Z-score: 570.3 E(): 7.1e-24 Smith-Waterman score: 435; 66.019 identity in 103 aa overlap.; 50S ribosomal protein L21 793343 rplU 4198997 rplU Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L21 YP_666912.1 793029 D 393115 CDS YP_666913.1 110670356 4199002 793374..793628 1 NC_008245.1 involved in the peptidyltransferase reaction during translation; 50S ribosomal protein L27 793628 rpmA 4199002 rpmA Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L27 YP_666913.1 793374 D 393115 CDS YP_666914.1 110670357 4199914 794015..794392 1 NC_008245.1 Similar to Q8DJY4 Tll1086 protein from Synechococcus elongatus (Thermosynechococcus elongatus) (126 aa). FASTA: opt: 516 Z-score: 715.9 E(): 5.5e-32 Smith-Waterman score: 516; 64.348 identity in 115 aa overlap. ORF ftt0774; hypothetical protein 794392 4199914 FTF0774 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666914.1 794015 D 393115 CDS YP_666915.1 110670358 4199226 complement(795628..796722) 1 NC_008245.1 Similar to Q8YGF6 Ribonuclease D from Brucella melitensis (385 aa). FASTA: opt: 538 Z-score: 584.5 E(): 1.2e-24 Smith-Waterman score: 538; 28.919 identity in 370 aa overlap.; ribonuclease D 796722 rnd 4199226 rnd Francisella tularensis subsp. tularensis FSC198 ribonuclease D YP_666915.1 795628 R 393115 CDS YP_666916.1 110670359 4200135 796831..798669 1 NC_008245.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision; excinuclease ABC subunit C 798669 uvrC 4200135 uvrC Francisella tularensis subsp. tularensis FSC198 excinuclease ABC subunit C YP_666916.1 796831 D 393115 CDS YP_666917.1 110670360 4199915 798736..799110 1 NC_008245.1 ORF ftt0778; hypothetical protein 799110 4199915 FTF0778 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666917.1 798736 D 393115 CDS YP_666918.1 110670361 4199635 join(799188..799514,799514..799972) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 799972 isftu1 4199635 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666918.1 799188 D 393115 CDS YP_666919.1 110670362 4199916 complement(800248..800517) 1 NC_008245.1 ORF ftt0780c; hypothetical protein 800517 4199916 FTF0780c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666919.1 800248 R 393115 CDS YP_666920.1 110670363 4199917 complement(800507..801220) 1 NC_008245.1 Similar to Q82UL5 Hypothetical protein from Nitrosomonas europaea (239 aa). FASTA: opt: 736 Z-score: 920.8 E(): 2.1e-43 Smith-Waterman score: 736; 54.680 identity in 203 aa overlap. The family of possible lysine decarboxylase proteins shares a highly conserved motif PGGXGTXXE that is probably functionally important. This protein has that motif. ORF ftt0781c; hypothetical protein 801220 4199917 FTF0781c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666920.1 800507 R 393115 CDS YP_666921.1 110670364 4199682 801506..802288 1 NC_008245.1 Similar to Q87YS1 Enoyl-(acyl-carrier-protein) reductase from Pseudomonas syringae (pv. tomato) (264 aa). FASTA: opt: 1071 Z-score: 1313.1 E(): 3e-65 Smith-Waterman score: 1071; 62.595 identity in 262 aa overlap.; enoyl-[acyl-carrier-protein] reductase (NADH) 802288 fabI 4199682 fabI Francisella tularensis subsp. tularensis FSC198 enoyl-[acyl-carrier-protein] reductase (NADH) YP_666921.1 801506 D 393115 CDS YP_666922.1 110670365 4199685 802435..803397 1 NC_008245.1 Similar to Q9CPJ8 Arylsulfatase from Pasteurella multocida (327 aa). FASTA: opt: 544 Z-score: 669.4 E(): 2.2e-29 Smith-Waterman score: 544; 31.420 identity in 331 aa overlap.; arylsulfatase 803397 ars 4199685 ars Francisella tularensis subsp. tularensis FSC198 arylsulfatase YP_666922.1 802435 D 393115 CDS YP_666923.1 110670366 4199918 803409..803738 1 NC_008245.1 ORF ftt0784; hypothetical protein 803738 4199918 FTF0784 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666923.1 803409 D 393115 CDS YP_666924.1 110670367 4199919 803726..804547 1 NC_008245.1 Similar to Q88K93 Conserved hypothetical protein (277 aa). FASTA: opt: 718 Z-score: 855.8 E(): 8.9e-40 Smith-Waterman score: 718; 42.308identity in 260 aa overlap. ORF ftt0785; hypothetical protein 804547 4199919 FTF0785 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666924.1 803726 D 393115 CDS YP_666925.1 110670368 4199920 804544..805242 1 NC_008245.1 ORF ftt0786; hypothetical protein 805242 4199920 FTF0786 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666925.1 804544 D 393115 CDS YP_666926.1 110670369 4199921 805253..805843 1 NC_008245.1 Similar to Q8EDN3 Conserved hypothetical protein from Shewanella oneidensis (197 aa). FASTA: opt: 435 Z-score: 540.9 E(): 3.1e-22 Smith-Waterman score: 435; 42.935 identity in 184 aa overlap. ORF ftt0787; DoxD-like family protein 805843 4199921 FTF0787 Francisella tularensis subsp. tularensis FSC198 DoxD-like family protein YP_666926.1 805253 D 393115 CDS YP_666927.1 110670370 4199508 complement(805847..806830) 1 NC_008245.1 Similar to Q8KLW6 Hypothetical protein from Pseudomonas stutzeri (Pseudomonas perfectomarina) (324 aa). FASTA: opt: 1095 Z-score: 1307.1 E(): 6.5e-65 Smith-Waterman score: 1095; 54.489 identity in 323 aa overlap. Homologs are involved in sialic acid capsule biosynthesis in some pathogenic strains of Escherichia coli.; arabinose phosphate isomerase 806830 kdsD 4199508 kdsD Francisella tularensis subsp. tularensis FSC198 arabinose phosphate isomerase YP_666927.1 805847 R 393115 CDS YP_666928.1 110670371 4200770 806955..807623 1 NC_008245.1 Similar to Q8EK14 Ribulose-phosphate 3-epimerase (225 aa). FASTA: opt: 953 Z-score: 1164.4 E(): 5.8e-57 Smith-Waterman score: 953; 65.766identity in 222 aa overlap.; D-ribulose-phosphate 3-epimerase 807623 rpe 4200770 rpe Francisella tularensis subsp. tularensis FSC198 D-ribulose-phosphate 3-epimerase YP_666928.1 806955 D 393115 CDS YP_666929.1 110670372 4199922 807604..808998 1 NC_008245.1 Similar to Q9FDJ5 Putative glycosyltransferase from Lactobacillus rhamnosus (466 aa). FASTA: opt: 733 Z-score: 876.0 E(): 6.7e-41 Smith-Waterman score: 733; 33.333 identity in 399 aa overlap. ORF ftt0790; sugar transferase 808998 4199922 FTF0790 Francisella tularensis subsp. tularensis FSC198 sugar transferase YP_666929.1 807604 D 393115 CDS YP_666930.1 110670373 4199208 808991..810010 1 NC_008245.1 UDP-glucose 4-epimerase 810010 galE 4199208 galE Francisella tularensis subsp. tularensis FSC198 UDP-glucose 4-epimerase YP_666930.1 808991 D 393115 CDS YP_666931.1 110670374 4199923 810014..811243 1 NC_008245.1 Similar to Q9HTC0 Glycosyltransferase WbpZ from Pseudomonas aeruginosa (381 aa). FASTA: opt: 621 Z-score: 721.7 E(): 2.6e-32 Smith-Waterman score: 1019; 38.725 identity in 408 aa overlap. ORF ftt0792; glycosyl transferases group 1 family protein 811243 4199923 FTF0792 Francisella tularensis subsp. tularensis FSC198 glycosyl transferases group 1 family protein YP_666931.1 810014 D 393115 CDS YP_666932.1 110670375 4199924 811240..812922 1 NC_008245.1 Similar to Q8DMA7 ATP-binding protein of ABC transporter from Synechococcus elongatus (Thermosynechococcus elongatus) (610 aa). FASTA: opt: 1084 Z-score: 1105.1 E(): 1.2e-53 Smith-Waterman score: 1084; 35.159 identity in 566 aa overlap. ORF ftt0793; ABC transporter ATP-binding and membrane protein 812922 4199924 FTF0793 Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding and membrane protein YP_666932.1 811240 D 393115 CDS YP_666933.1 110670376 4199925 812936..814222 1 NC_008245.1 Similar to AAP76675 Phosphoserine phosphatase (EC 3.1.3.3) from Helicobacter hepaticus (199 aa). FASTA: opt: 751 Z-score: 908.2 E(): 1.1e-42 Smith-Waterman score: 751; 55.385 identity in 195 aa overlap (18-212:4-198) AND to AAP76674 Hypothetical protein from Helicobacter hepaticus (213 aa). FASTA: opt: 823 Z-score: 994.3 E(): 1.7e-47 Smith-Waterman score: 823; 57.212 identity in 208 aa overlap (221-428:6-213). Seems to be two genes in some organisms. ORF ftt0794; hypothetical protein 814222 4199925 FTF0794 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666933.1 812936 D 393115 CDS YP_666934.1 110670377 4199926 814161..814844 1 NC_008245.1 Similar to AAP76671 Hypothetical protein cfa from Helicobacter hepaticus (210 aa). FASTA: opt: 627 Z-score: 745.4 E(): 1.3e-33 Smith-Waterman score: 627; 44.660 identity in 206 aa overlap. ORF ftt0795; hypothetical protein 814844 4199926 FTF0795 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666934.1 814161 D 393115 CDS YP_666935.1 110670378 4199927 814848..815609 1 NC_008245.1 ORF ftt0796; hypothetical protein 815609 4199927 FTF0796 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666935.1 814848 D 393115 CDS YP_666936.1 110670379 4199928 815618..816577 1 NC_008245.1 Similar to O85000 Galactosyl transferase from Streptococcus pneumoniae (323 aa). FASTA: opt: 577 Z-score: 682.7 E(): 3.9e-30 Smith-Waterman score: 577; 40.171 identity in 234 aa overlap. Same homolog as for next downstream ORF. ORF ftt0797; glycosyl transferase family protein 816577 4199928 FTF0797 Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_666936.1 815618 D 393115 CDS YP_666937.1 110670380 4199929 816594..817601 1 NC_008245.1 Similar to O85000 Galactosyl transferase from Streptococcus pneumoniae (323 aa). FASTA: opt: 499 Z-score: 609.1 E(): 4.9e-26 Smith-Waterman score: 499; 34.454 identity in 238 aa overlap. Same homolog as for next upstream ORF. ORF ftt0798; glycosyl transferase family protein 817601 4199929 FTF0798 Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_666937.1 816594 D 393115 CDS YP_666938.1 110670381 4199930 817604..818617 1 NC_008245.1 Similar to Q00481 Second mannosyl transferase from Salmonella enterica (336 aa). FASTA: opt: 651 Z-score: 775.2 E(): 2.7e-35 Smith-Waterman score: 651; 35.455 identity in 330 aa overlap. ORF ftt0799; glycosyl transferases group 1 family protein 818617 4199930 FTF0799 Francisella tularensis subsp. tularensis FSC198 glycosyl transferases group 1 family protein YP_666938.1 817604 D 393115 CDS YP_666939.1 110670382 4199931 818636..819298 1 NC_008245.1 Similar to Q8FV27 Hydrolase, haloacid dehalogenase-like family from Brucella suis (234 aa). FASTA: opt: 422 Z-score: 503.3 E(): 3.8e-20 Smith-Waterman score: 422; 33.486identity in 218 aa overlap. ORF ftt0800; haloacid dehalogenase-like hydrolase family protein 819298 4199931 FTF0800 Francisella tularensis subsp. tularensis FSC198 haloacid dehalogenase-like hydrolase family protein YP_666939.1 818636 D 393115 CDS YP_666940.1 110670383 4199969 complement(819580..820686) 1 NC_008245.1 Similar to Q9YG89 310AA long hypothetical sugar kinase from Aeropyrum pernix (310 aa). FASTA: opt: 245 Z-score: 292.6 E(): 2.1e-08 Smith-Waterman score: 311; 25.795 identity in 283 aa overlap. ORF ftt0801c; carbohydrate/purine kinase pfkB family protein 820686 4199969 FTF0801c Francisella tularensis subsp. tularensis FSC198 carbohydrate/purine kinase pfkB family protein YP_666940.1 819580 R 393115 CDS YP_666941.1 110670384 4200274 820889..821707 1 NC_008245.1 Similar to CPHB_SYNY8 (P56946) Cyanophycinase from Synechocystis sp. (strain PCC 6308) (272 aa). FASTA: opt: 701 Z-score: 857.3 E(): 7.4e-40 Smith-Waterman score: 701; 41.667 identity in 264 aa overlap.; cyanophycinase 821707 cphB 4200274 cphB Francisella tularensis subsp. tularensis FSC198 cyanophycinase YP_666941.1 820889 D 393115 CDS YP_666942.1 110670385 4199423 821923..822786 1 NC_008245.1 Similar to Q9ABX3 Asparaginase family protein from Caulobacter crescentus (292 aa). FASTA: 146 opt: 465 Z-score: 567.7 E(): 1e-23 Smith-Waterman score: 465; 35.606 identity in 264 aa overlap.; asparaginase 822786 ans 4199423 ans Francisella tularensis subsp. tularensis FSC198 asparaginase YP_666942.1 821923 D 393115 CDS YP_666943.1 110670386 4199970 823458..824714 1 NC_008245.1 Similar to O54543 (O54543) CITA from Legionella pneumophila (418 aa). FASTA: opt: 905 Z-score: 915.1 E(): 4.5e-43 Smith-Waterman score: 905; 33.254 identity in 418 aa overlap. ORF ftt0804; major facilitator transporter 824714 4199970 FTF0804 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_666943.1 823458 D 393115 CDS YP_666944.1 110670387 4200313 824790..826007 1 NC_008245.1 Similar to CAD71904 Capsule biosynthesis protein CapB from Rhodopirellula baltica (441 aa). FASTA: opt: 1155 Z-score: 1414.5 E(): 6.8e-71 Smith-Waterman score: 1156; 46.763 identity (50.914 ungapped) in 417 aa overlap.; capsule biosynthesis protein capB 826007 capB 4200313 capB Francisella tularensis subsp. tularensis FSC198 capsule biosynthesis protein capB YP_666944.1 824790 D 393115 CDS YP_666945.1 110670388 4200314 826032..826496 1 NC_008245.1 Similar to Q8REZ4 CapC protein from Fusobacterium nucleatum (152 aa). FASTA: opt: 360 Z-score: 420.9 E(): 1.5e-15 Smith-Waterman score: 360; 35.616 identity in 146 aa overlap.; capsule biosynthesis protein CapC 826496 capC 4200314 capC Francisella tularensis subsp. tularensis FSC198 capsule biosynthesis protein CapC YP_666945.1 826032 D 393115 CDS YP_666946.1 110670389 4199971 826498..827709 1 NC_008245.1 Similar to CAD71902 Hypothetical protein from Rhodopirellula baltica (404 aa). FASTA: opt: 477 Z-score: 563.9 E(): 1.6e-23 Smith-Waterman score: 553; 31.633 identity in 392 aa overlap. ORF ftt0807; hypothetical protein 827709 4199971 FTF0807 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666946.1 826498 D 393115 CDS YP_666947.1 110670390 4199083 827769..829892 1 NC_008245.1 Similar to SPOT_ECOLI Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase from Escherichia coli (702 aa). FASTA: opt: 1849 Z-score: 2107.7 E(): 1.7e-109 Smith-Waterman score: 1849; 41.210 identity in 711 aa overlap.; guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase/(p)ppGpp synthase 829892 spoT 4199083 spoT Francisella tularensis subsp. tularensis FSC198 guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase/(p)ppGpp synthase YP_666947.1 827769 D 393115 CDS YP_666948.1 110670391 4200607 complement(829900..830502) 1 NC_008245.1 involved in a recombinational process of DNA repair, independent of the recBC complex; recombination protein RecR 830502 recR 4200607 recR Francisella tularensis subsp. tularensis FSC198 recombination protein RecR YP_666948.1 829900 R 393115 CDS YP_666949.1 110670392 4200701 complement(830502..830840) 1 NC_008245.1 Similar to YBAB_ECOLI Hypothetical UPF0133 protein ybaB from Escherichia coli (109 aa). FASTA: opt: 332 Z-score: 403.8 E(): 1.3e-14 Smith-Waterman score: 332; 49.074identity in 108 aa overlap.; hypothetical protein 830840 ybaB 4200701 ybaB Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666949.1 830502 R 393115 CDS YP_666950.1 110670393 4200110 complement(830850..831965) 1 NC_008245.1 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis; undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase 831965 murG 4200110 murG Francisella tularensis subsp. tularensis FSC198 undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase YP_666950.1 830850 R 393115 CDS YP_666951.1 110670394 4199278 832069..832449 1 NC_008245.1 Similar to GCS1_SULTO Probable glycine cleavage system H from Sulfolobus tokodaii (142 aa). FASTA: opt: 397 Z-score: 525.8 E(): 2.1e-21 Smith-Waterman score: 397; 43.089 identity in 123 aa overlap.; glycine cleavage system protein H 832449 gcvH2 4199278 gcvH2 Francisella tularensis subsp. tularensis FSC198 glycine cleavage system protein H YP_666951.1 832069 D 393115 CDS YP_666952.1 110670395 4199972 832439..832735 1 NC_008245.1 ORF ftt0813; hypothetical protein 832735 4199972 FTF0813 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666952.1 832439 D 393115 CDS YP_666953.1 110670396 4199973 complement(832821..833420) 1 NC_008245.1 ORF ftt0814c; hypothetical protein 833420 4199973 FTF0814c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666953.1 832821 R 393115 CDS YP_666954.1 110670397 4199974 complement(833428..833676) 1 NC_008245.1 ORF ftt0815c; hypothetical protein 833676 4199974 FTF0815c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666954.1 833428 R 393115 CDS YP_666955.1 110670398 4199975 complement(833720..834613) 1 NC_008245.1 Similar to Q8EHY2 Chitin-binding protein,putative from Shewanella oneidensis (491 aa). FASTA: opt: 532 Z-score: 628.3 E(): 4.2e-27 Smith-Waterman score: 532; 43.500 identity in 200 aa overlap. ORF ftt0816c; chitin binding protein 834613 4199975 FTF0816c Francisella tularensis subsp. tularensis FSC198 chitin binding protein YP_666955.1 833720 R 393115 CDS YP_666956.1 110670399 4199143 835053..836957 1 NC_008245.1 catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); threonyl-tRNA synthetase 836957 thrS 4199143 thrS Francisella tularensis subsp. tularensis FSC198 threonyl-tRNA synthetase YP_666956.1 835053 D 393115 CDS YP_666957.1 110670400 4199611 837033..837500 1 NC_008245.1 Similar to IF3_ECOLI Translation initiation factor IF-3 from Escherichia coli (180 aa). FASTA: opt: 674 Z-score: 836.1 E(): 1.1e-38 Smith-Waterman score: 674; 64.103 identity in 156 aa overlap.; translation initiation factor IF-3 837500 infC 4199611 infC Francisella tularensis subsp. tularensis FSC198 translation initiation factor IF-3 YP_666957.1 837033 D 393115 CDS YP_666958.1 110670401 4200324 837543..837740 1 NC_008245.1 Similar to RL35_ECOLI 50S ribosomal protein L35 from Escherichia coli (64 aa). FASTA: opt: 247 Z-score: 385.9 E(): 1.3e-13 Smith-Waterman score: 247; 62.500 identity in 64 aa overlap.; 50S ribosomal protein L35 837740 rpmI 4200324 rpmI Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L35 YP_666958.1 837543 D 393115 CDS YP_666959.1 110670402 4198996 837776..838132 1 NC_008245.1 binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit; 50S ribosomal protein L20 838132 rplT 4198996 rplT Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L20 YP_666959.1 837776 D 393115 CDS YP_666960.1 110670403 4199976 838174..838878 1 NC_008245.1 Similar to Q9KRB9 Hypothetical protein VC1723 from Vibrio cholerae (229 aa). FASTA: opt: 344 Z-score: 394.7 E(): 4.3e-14 Smith-Waterman score: 344; 29.075 identity in 227 aa overlap. ORF ftt0821; hypothetical protein 838878 4199976 FTF0821 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666960.1 838174 D 393115 CDS YP_666961.1 110670404 4199636 join(839170..839496,839496..839954) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 839954 isftu1 4199636 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_666961.1 839170 D 393115 CDS YP_666962.1 110670405 4199977 840178..840825 1 NC_008245.1 Similar to Q9K8B7 Mutator MutT protein from Bacillus halodurans (207 aa). FASTA: opt: 413 Z-score: 505.4 E(): 2.9e-20 Smith-Waterman score: 413; 36.649identity in 191 aa overlap. ORF ftt0823; MutT protein 840825 4199977 FTF0823 Francisella tularensis subsp. tularensis FSC198 MutT protein YP_666962.1 840178 D 393115 CDS YP_666963.1 110670406 4199686 840843..841196 1 NC_008245.1 Similar to Q8CPW9 Arsenate reductase from Staphylococcus epidermidis (117 aa). FASTA: opt: 276 Z-score: 376.2 E(): 4.6e-13 Smith-Waterman score: 276; 32.759 identity in 116 aa overlap.; arsenate reductase 841196 arsC1 4199686 arsC1 Francisella tularensis subsp. tularensis FSC198 arsenate reductase YP_666963.1 840843 D 393115 CDS YP_666964.1 110670407 4199978 complement(841220..841555) 1 NC_008245.1 hypothetical lipoprotein, no significant homologs. ORF ftt0825c; hypothetical protein 841555 4199978 FTF0825c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666964.1 841220 R 393115 CDS YP_666965.1 110670408 4199979 complement(841732..841977) 1 NC_008245.1 ORF ftt0826c; lipoprotein 841977 4199979 FTF0826c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666965.1 841732 R 393115 CDS YP_666966.1 110670409 4199980 complement(842055..843368) 1 NC_008245.1 Similar to YIEG_ECOLI Hypothetical protein yieG from Escherichia coli (445 aa). FASTA: opt: 945 Z-score: 1037.7 E(): 6.6e-50 Smith-Waterman score: 945; 34.633 identity in 436 aa overlap. ORF ftt0827c; hypothetical protein 843368 4199980 FTF0827c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666966.1 842055 R 393115 CDS YP_666967.1 110670410 4199982 complement(845033..846748) 1 NC_008245.1 Similar to Q845W9 Aspartate:alanine antiporter from Pseudomonas dacunhae (561 aa). FASTA: opt: 1359 Z-score: 1502.2 bits: 287.8 E(): 8.8e-76 Smith-Waterman score: 1359; 38.351 identity in 558 aa overlap. ORF ftt0829c; aspartate:alanine antiporter 846748 4199982 FTF0829c Francisella tularensis subsp. tularensis FSC198 aspartate:alanine antiporter YP_666967.1 845033 R 393115 CDS YP_666968.1 110670411 4199984 complement(847903..849156) 1 NC_008245.1 Weakly similar to Q8A8H3 Outer membrane protein,OmpA family from Bacteroides thetaiotaomicron (266 aa). FASTA: opt: 184 Z-score: 220.7 E(): 0.00021 Smith-Waterman score: 184; 29.299 identity in 157 aa overlap. ORF ftt0831c; OmpA family protein 849156 4199984 FTF0831c Francisella tularensis subsp. tularensis FSC198 OmpA family protein YP_666968.1 847903 R 393115 CDS YP_666969.1 110670412 4199411 849348..849812 1 NC_008245.1 Similar to FKBX_ECOLI (P22563) FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase from Escherichia coli (148 aa). FASTA: opt: 357 Z-score: 475.0 E(): 1.4e-18 Smith-Waterman score: 357; 38.129 identity in 139 aa overlap.; FKBP-type 16 kDa peptidyl-prolyl cis-transisomerase 849812 fkpB 4199411 fkpB Francisella tularensis subsp. tularensis FSC198 FKBP-type 16 kDa peptidyl-prolyl cis-transisomerase YP_666969.1 849348 D 393115 CDS YP_666970.1 110670413 4199304 849793..850749 1 NC_008245.1 catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 850749 ispH 4199304 ispH Francisella tularensis subsp. tularensis FSC198 4-hydroxy-3-methylbut-2-enyl diphosphate reductase YP_666970.1 849793 D 393115 CDS YP_666971.1 110670414 4199439 850751..851113 1 NC_008245.1 Similar to CHMU_BACSU Chorismate mutase from Bacillus subtilis (127 aa). FASTA: opt: 360 Z-score: 492.8 E(): 1.5e-19 Smith-Waterman score: 360; 48.305identity in 118 aa overlap.; chorismate mutase 851113 aroQ 4199439 aroQ Francisella tularensis subsp. tularensis FSC198 chorismate mutase YP_666971.1 850751 D 393115 CDS YP_666972.1 110670415 4199985 851122..851943 1 NC_008245.1 Similar to Q8EBE8 CDP-diacylglycerol--serine O-phosphatidyltransferase from Shewanella oneidensis (269 aa). FASTA: opt: 456 Z-score: 550.1 E(): 9.5e-23 Smith-Waterman score: 564; 37.209 identity in 258 aa overlap. ORF ftt0835; CDP-alcohol phosphatidyltransferase 851943 4199985 FTF0835 Francisella tularensis subsp. tularensis FSC198 CDP-alcohol phosphatidyltransferase YP_666972.1 851122 D 393115 CDS YP_666973.1 110670416 4199986 complement(851948..852172) 1 NC_008245.1 ORF ftt0836c; hypothetical protein 852172 4199986 FTF0836c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666973.1 851948 R 393115 CDS YP_666974.1 110670417 4199151 852313..853032 1 NC_008245.1 Similar to TOLQ_ECOLI TolQ protein from Escherichia coli (230 aa). FASTA: opt: 568 Z-score: 690.3 E(): 1.5e-30 Smith-Waterman score: 568; 38.991 identity in 218 aa overlap.; TolQ protein 853032 tolQ 4199151 tolQ Francisella tularensis subsp. tularensis FSC198 TolQ protein YP_666974.1 852313 D 393115 CDS YP_666975.1 110670418 4199857 853042..853488 1 NC_008245.1 Similar to TOLR_ECOLI TolR protein from Escherichia coli (142 aa). FASTA: opt: 303 Z-score: 379.2 bits: 76.1 E(): 3.2e-13 Smith-Waterman score: 303; 39.259identity in 135 aa overlap.; TolR protein 853488 tolR 4199857 tolR Francisella tularensis subsp. tularensis FSC198 TolR protein YP_666975.1 853042 D 393115 CDS YP_666976.1 110670419 4199148 853475..854386 1 NC_008245.1 FTF0839 Contains similarity to Pfam domain PF06519 (TolA protein), Score=-66.2, E-value=1.4e-05; tolA protein 854386 tolA 4199148 tolA Francisella tularensis subsp. tularensis FSC198 tolA protein YP_666976.1 853475 D 393115 CDS YP_666977.1 110670420 4199149 854386..855705 1 NC_008245.1 Similar to TOLB_ECOLI TolB protein precursor from Eschericia coli (430 aa). FASTA: opt: 692 Z-score: 785.2 E(): 7.6e-36 Smith-Waterman score: 717; 31.685identity in 445 aa overlap. TolB appears more highly conserved in other species: potential paralog; TolB protein precursor 855705 tolB 4199149 tolB Francisella tularensis subsp. tularensis FSC198 TolB protein precursor YP_666977.1 854386 D 393115 CDS YP_666978.1 110670421 4199987 855709..855960 1 NC_008245.1 ORF ftt0841; lipoprotein 855960 4199987 FTF0841 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_666978.1 855709 D 393115 CDS YP_666979.1 110670422 4200006 855979..856602 1 NC_008245.1 Similar to PAL_ECOLI Peptidoglycan-associated lipoprotein from Escherichia coli (173 aa). FASTA: opt: 306 Z-score: 383.2 E(): 1.9e-13 Smith-Waterman score: 306; 33.333 identity in 162 aa overlap. ORF ftt0842; peptidoglycan-associated lipoprotein 856602 4200006 FTF0842 Francisella tularensis subsp. tularensis FSC198 peptidoglycan-associated lipoprotein YP_666979.1 855979 D 393115 CDS YP_666980.1 110670423 4200009 858830..859105 1 NC_008245.1 Similar to Q9K315 Hypothetical protein VCA0343 OR VCA029 from Vibrio cholerae (83 aa). FASTA: opt: 250 Z-score: 356.2 E(): 6e-12 Smith-Waterman score: 250; 58.462 identity (58.4620ngapped) in 65 aa overlap. ORF ftt0845; hypothetical protein 859105 4200009 FTF0845 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666980.1 858830 D 393115 CDS YP_666981.1 110670424 4200010 859086..860585 1 NC_008245.1 Similar to Q9KS67 Deoxyribodipyrimidine photolyase,putative from Vibrio cholerae (504 aa). FASTA: opt: 1071 Z-score: 1263.0 E(): 1.8e-62 Smith-Waterman score: 1097; 37.817 identity in 513 aa overlap. ORF ftt0846; deoxyribodipyrimidine photolyase 860585 4200010 FTF0846 Francisella tularensis subsp. tularensis FSC198 deoxyribodipyrimidine photolyase YP_666981.1 859086 D 393115 CDS YP_666982.1 110670425 4200011 860639..862114 1 NC_008245.1 Similar to Q8U6P2 Hypothetical protein Atu4765 (507 aa). FASTA: opt: 1800 Z-score: 2192.9 415.3 E(): 3e-114 Smith-Waterman score: 1800; 53.608identity in 485 aa overlap. ORF ftt0847; hypothetical protein 862114 4200011 FTF0847 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666982.1 860639 D 393115 CDS YP_666983.1 110670426 4200012 862111..862248 1 NC_008245.1 Similar to Q8P3G1 Hypothetical protein XCC4110 from Xanthomonas campestris (51 aa). FASTA: opt: 243 Z-score: 366.5 E(): 1.6e-12 Smith-Waterman score: 243; 63.636 identity in 44 aa overlap ORF ftt0848; hypothetical protein 862248 4200012 FTF0848 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666983.1 862111 D 393115 CDS YP_666984.1 110670427 4200013 862374..863303 1 NC_008245.1 Similar to Q81YH5 Bile acid transporter family protein from Bacillus anthracis (322 aa). FASTA: initn: 551 init1: 399 opt: 605 Z-score: 641.1 E(): 8.1e-28 Smith-Waterman score: 605; 32.432identity in 296 aa overlap. ORF ftt0849; bile acid symporter family protein 863303 4200013 FTF0849 Francisella tularensis subsp. tularensis FSC198 bile acid symporter family protein YP_666984.1 862374 D 393115 CDS YP_666985.1 110670428 4200014 863353..863976 1 NC_008245.1 Similar to Q930D7 Hypothetical protein RA0261 Rhizobium meliloti (222 aa). FASTA: opt: 521 Z-score: 614.9 E(): 2.3e-26 Smith-Waterman score: 521; 42.424 identity in 198 aa overlap. ORF ftt0850; hypothetical protein 863976 4200014 FTF0850 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666985.1 863353 D 393115 CDS YP_666986.1 110670429 4200015 864000..864758 1 NC_008245.1 Similar to Q83C04 Membrane protein, putative from Coxiella burnetii (263 aa). FASTA: opt: 338 Z-score: 387.6 E(): 1.1e-13 Smith-Waterman score: 341; 28.625 identity in 269 aa overlap. ORF ftt0851; hypothetical protein 864758 4200015 FTF0851 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666986.1 864000 D 393115 CDS YP_666987.1 110670430 4200017 866452..867690 1 NC_008245.1 Similar to Q8PY17 Arsenite permease from Methanosarcina mazei (420 aa). FASTA: opt: 1294 Z-score: 1359.3 E(): 8e-68 Smith-Waterman score: 1294; 49.144 identity in 409 aa overlap. ORF ftt0853; arsenite permease family protein 867690 4200017 FTF0853 Francisella tularensis subsp. tularensis FSC198 arsenite permease family protein YP_666987.1 866452 D 393115 CDS YP_666988.1 110670431 4200018 complement(867669..868091) 1 NC_008245.1 Similar to Q8EM34 Hypothetical conserved protein from Oceanobacillus iheyensis (123 aa). FASTA: opt: 271 Z-score: 341.6 E(): 3.9e-11 Smith-Waterman score: 271; 37.190 identity in 121 aa overlap. ORF ftt0854c; hypothetical protein 868091 4200018 FTF0854c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666988.1 867669 R 393115 CDS YP_666989.1 110670432 4200019 complement(868111..868470) 1 NC_008245.1 Weakly similar to Q9KR35 Hypothetical protein VC1812 from Vibrio cholerae (139 aa). FASTA: opt: 184 Z-score: 256.0 E(): 2.3e-06 Smith-Waterman score: 184; 29.245 identity in 106 aa overlap. ORF ftt0855c; hypothetical protein 868470 4200019 FTF0855c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666989.1 868111 R 393115 CDS YP_666990.1 110670433 4198976 complement(868484..869338) 1 NC_008245.1 UbiA prenyltransferase family catalyzes the transfer of a prenyl group to various acceptors with hydrophobic ring structures in the biosynthesis of respiratory quinones, hemes, chlorophylls, vitamin E, and shikonin; 4-hydroxybenzoate polyprenyltransferase 869338 ubiA 4198976 ubiA Francisella tularensis subsp. tularensis FSC198 4-hydroxybenzoate polyprenyltransferase YP_666990.1 868484 R 393115 CDS YP_666991.1 110670434 4200020 complement(869341..869847) 1 NC_008245.1 Weakly similar to similar to Q83F94 Chorismate--pyruvate lyase, putative from Coxiella burnetii (185 aa). FASTA: opt: 178 Z-score: 231.0 E(): 5.6e-05 Smith-Waterman score: 178; 27.215identity in 158 aa overlap ORF ftt0857c; hypothetical protein 869847 4200020 FTF0857c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666991.1 869341 R 393115 CDS YP_666992.1 110670435 4200771 869946..870653 1 NC_008245.1 RNase PH; tRNA nucleotidyltransferase; forms hexamers in Bacillus subtilis; phosphoroltic 3'-5' exoribonuclease; involved in maturation of tRNA precursors and removes terminal nucleotides near CCA acceptor arms of mature tRNAs; ribonuclease PH 870653 rph 4200771 rph Francisella tularensis subsp. tularensis FSC198 ribonuclease PH YP_666992.1 869946 D 393115 CDS YP_666993.1 110670436 4200021 complement(870707..871030) 1 NC_008245.1 ORF ftt0859c; hypothetical protein 871030 4200021 FTF0859c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666993.1 870707 R 393115 CDS YP_666994.1 110670437 4200022 complement(871135..871386) 1 NC_008245.1 ORF ftt0860c; hypothetical protein 871386 4200022 FTF0860c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_666994.1 871135 R 393115 CDS YP_666995.1 110670438 4200023 complement(871449..872042) 1 NC_008245.1 Similar to FMA2_BACNO (P17824) Fimbrial protein precursor (Pilin) from Bacteroides nodosus (Dichelobacter nodosus) (160 aa). FASTA: opt: 169 Z-score: 222.1 E(): 0.00018 Smith-Waterman score: 169; 34.646 identity in 127 aa overlap The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above, but shows a weak global homology. ORF ftt0861c; Type IV pili fiber building block protein 872042 4200023 FTF0861c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_666995.1 871449 R 393115 CDS YP_666996.1 110670439 4200648 complement(872147..873220) 1 NC_008245.1 putative metalloprotease; heat shock protein HtpX 873220 htpX 4200648 htpX Francisella tularensis subsp. tularensis FSC198 heat shock protein HtpX YP_666996.1 872147 R 393115 CDS YP_666997.1 110670440 4200024 complement(873224..873799) 1 NC_008245.1 Similar to Q83DZ3 LemA protein from Coxiella burnetii (192 aa) initn: 723 init1: 655 opt: 732 Z-score: 803.2 bits: 155.4 E(): 7.6e-37 Smith-Waterman score: 732; 60.417 identity in 192 aa overlap. ORF ftt0863c; LemA-like protein 873799 4200024 FTF0863c Francisella tularensis subsp. tularensis FSC198 LemA-like protein YP_666997.1 873224 R 393115 CDS YP_666998.1 110670441 4200025 complement(873847..874743) 1 NC_008245.1 Similar to Q87XZ5 Transcriptional regulator, LysR family, from Pseudomonas syringae (308 aa). FASTA: opt: 591 Z-score: 726.5 E(): 1.4e-32 Smith-Waterman score: 591; 34.948 identity in 289 aa overlap. ORF ftt0864c; LysR family transcriptional regulator 874743 4200025 FTF0864c Francisella tularensis subsp. tularensis FSC198 LysR family transcriptional regulator YP_666998.1 873847 R 393115 CDS YP_666999.1 110670442 4199291 876718..877461 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 877461 isftu2 4199291 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_666999.1 876718 D 393115 CDS YP_667000.1 110670443 4199594 complement(878966..879331) 1 NC_008245.1 Similar to Q9RA93 ArsR from Sinorhizobium sp. As4 (115 aa). FASTA: opt: 185 Z-score: 259.0 bits: 53.3 E(): 1.6e-06 Smith-Waterman score: 185; 27.184 identity in 103 aa overlap. ORF ftt0868c; arsenical restistance operon repressor 879331 4199594 FTF0868c Francisella tularensis subsp. tularensis FSC198 arsenical restistance operon repressor YP_667000.1 878966 R 393115 CDS YP_667001.1 110670444 4199595 879474..880127 1 NC_008245.1 ORF ftt0869; hypothetical protein 880127 4199595 FTF0869 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667001.1 879474 D 393115 CDS YP_667002.1 110670445 4199596 complement(880139..880270) 1 NC_008245.1 ORF ftt0870c; hypothetical protein 880270 4199596 FTF0870c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667002.1 880139 R 393115 CDS YP_667003.1 110670446 4200296 880447..881445 1 NC_008245.1 NAD(P)+; catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate; NAD(P)H-dependent glycerol-3-phosphate dehydrogenase 881445 gpsA 4200296 gpsA Francisella tularensis subsp. tularensis FSC198 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase YP_667003.1 880447 D 393115 CDS YP_667004.1 110670447 4199597 complement(881446..881982) 1 NC_008245.1 ORF ftt0872c; hypothetical protein 881982 4199597 FTF0872c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667004.1 881446 R 393115 CDS YP_667005.1 110670448 4200595 complement(881987..883357) 1 NC_008245.1 Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents; DNA repair protein RadA 883357 radA 4200595 radA Francisella tularensis subsp. tularensis FSC198 DNA repair protein RadA YP_667005.1 881987 R 393115 CDS YP_667006.1 110670449 4199598 complement(883365..884135) 1 NC_008245.1 ORF ftt0874c; hypothetical protein 884135 4199598 FTF0874c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667006.1 883365 R 393115 CDS YP_667007.1 110670450 4199599 complement(884132..884731) 1 NC_008245.1 Similar to YOHD_ECOLI (P33366) Hypothetical protein yohD from Escherichia coli (192 aa). FASTA: opt: 311 Z-score: 379.7 bits: 77.1 E(): 2.9e-13 Smith-Waterman score: 311; 24.870 identity (25.806 ungapped) in 193 aa overlap. ORF ftt0875c; hypothetical protein 884731 4199599 FTF0875c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667007.1 884132 R 393115 CDS YP_667008.1 110670451 4199433 complement(884734..885792) 1 NC_008245.1 catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis; chorismate synthase 885792 aroC 4199433 aroC Francisella tularensis subsp. tularensis FSC198 chorismate synthase YP_667008.1 884734 R 393115 CDS YP_667009.1 110670452 4199600 complement(885957..886586) 1 NC_008245.1 ORF ftt0877c; hypothetical protein 886586 4199600 FTF0877c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667009.1 885957 R 393115 CDS YP_667010.1 110670453 4200102 complement(886605..887117) 1 NC_008245.1 this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; provides protection against oxidative stress; methionine sulfoxide reductase B 887117 msrB 4200102 msrB Francisella tularensis subsp. tularensis FSC198 methionine sulfoxide reductase B YP_667010.1 886605 R 393115 CDS YP_667011.1 110670454 4199078 887278..887835 1 NC_008245.1 superoxide dismutase (Cu-Zn) precusor. Identical to SODC_FRATU from F. tularensis LVS.; superoxide dismuate (Cu-Zn) precusor 887835 sodC 4199078 sodC Francisella tularensis subsp. tularensis FSC198 superoxide dismuate (Cu-Zn) precusor YP_667011.1 887278 D 393115 CDS YP_667012.1 110670455 4199602 complement(888603..889997) 1 NC_008245.1 Similar to AAP09927 Arginine permease from Bacillus cereus (474 aa). FASTA: opt: 1041 Z-score: 1224.1 E(): 2.5e-60 Smith-Waterman score: 1041; 35.604 identity in 455 aa overlap. ORF ftt0881c; amino acid permease 889997 4199602 FTF0881c Francisella tularensis subsp. tularensis FSC198 amino acid permease YP_667012.1 888603 R 393115 CDS YP_667013.1 110670456 4199689 complement(891762..892952) 1 NC_008245.1 catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate; aspartate aminotransferase 892952 aspC1 4199689 aspC1 Francisella tularensis subsp. tularensis FSC198 aspartate aminotransferase YP_667013.1 891762 R 393115 CDS YP_667014.1 110670457 4199605 893241..894020 1 NC_008245.1 Similar to Q8D6R6 Predicted Co/Zn/Cd cation transporter from Vibrio vulnificus (297 aa). FASTA: opt: 344 Z-score: 414.1 E(): 3.3e-15 Smith-Waterman score: 344; 25.296 identity in 253 aa overlap. ORF ftt0885; cation transporter 894020 4199605 FTF0885 Francisella tularensis subsp. tularensis FSC198 cation transporter YP_667014.1 893241 D 393115 CDS YP_667015.1 110670458 4199606 894126..895775 1 NC_008245.1 Similar to P52118 DNA repair protein recN (Recombination protein (554 aa) fasta scores; opt: 1371 Z-score: 1221.7 E(): 3.4e-60 Smith-Waterman score: 1371; 39.674 identity in 552 aa overlap. ORF ftt0886; DNA repair protein recN 895775 4199606 FTF0886 Francisella tularensis subsp. tularensis FSC198 DNA repair protein recN YP_667015.1 894126 D 393115 CDS YP_667016.1 110670459 4199607 complement(896347..896721) 1 NC_008245.1 ORF ftt0887c; hypothetical protein 896721 4199607 FTF0887c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667016.1 896347 R 393115 CDS YP_667017.1 110670460 4199608 complement(896714..897310) 1 NC_008245.1 Similar to Q59607 Pilin precursor from Neisseria gonorrhoeae (169 aa). FASTA: opt: 244 Z-score: 305.7 E(): 3.5e-09 Smith-Waterman score: 244; 30.435 identity in 138 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0888c; Type IV pili fiber building block protein 897310 4199608 FTF0888c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_667017.1 896714 R 393115 CDS YP_667018.1 110670461 4199363 complement(897980..898420) 1 NC_008245.1 Similar to Q820M2 Fimbrial protein pilin:bacterial general secretion pathway protein H from Nitrosomonas europaea (136 aa). FASTA: opt: 216 Z-score: 280.1 E(): 1e-07 Smith-Waterman score: 218; 32.639 identity in 144 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0889c; Type IV pili fiber building block protein 898420 4199363 FTF0889c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_667018.1 897980 R 393115 CDS YP_667019.1 110670462 4199364 complement(898551..898958) 1 NC_008245.1 Similar to Q8Y1X9 Type 4 fimbrial pilin signal peptide protein from Ralstonia solanacearum (168 aa). FASTA: opt: 278 Z-score: 327.6 E(): 2.1e-10 Smith-Waterman score: 292; 38.095identity in 147 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW,pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above. ORF ftt0890c; Type IV pili fiber building block protein 898958 4199364 FTF0890c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_667019.1 898551 R 393115 CDS YP_667020.1 110670463 4199365 899342..900061 1 NC_008245.1 Similar to Q98NS1 Hypothetical protein mlr0012 from Rhizobium loti (244 aa). FASTA: opt: 327 Z-score: 404.4 bits: 82.2 E(): 1.2e-14 Smith-Waterman score: 327; 32.308 identity in 195 aa overlap. ORF ftt0891; hypothetical protein 900061 4199365 FTF0891 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667020.1 899342 D 393115 CDS YP_667021.1 110670464 4199416 900200..901048 1 NC_008245.1 catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate; bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase 901048 folD 4199416 folD Francisella tularensis subsp. tularensis FSC198 bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase YP_667021.1 900200 D 393115 CDS YP_667022.1 110670465 4199122 901282..902325 1 NC_008245.1 catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis; phosphoribosylaminoimidazole synthetase 902325 purM 4199122 purM Francisella tularensis subsp. tularensis FSC198 phosphoribosylaminoimidazole synthetase YP_667022.1 901282 D 393115 CDS YP_667023.1 110670466 4199116 902325..904637 1 NC_008245.1 Amino acids 1-327 (PurC) similar to AAR06292 SAICAR synthetase from Nicotiana tabacum (399 aa). BLAST: Expect = e-105 Identities = 187/322 (58), Positives = 236/322 (73), Gaps = 6/322 (1). Amino acids 328-770 (PurD) similar to ZP_00077753 Phosphoribosylamine-glycine ligase from Methanosarcina barkeri (433). BLAST: Expect = e-108 Identities = 204/436 (46), Positives = 285/436 (65), Gaps = 3/436 (0); fusion protein PurC/PurD 904637 purCD 4199116 purCD Francisella tularensis subsp. tularensis FSC198 fusion protein PurC/PurD YP_667023.1 902325 D 393115 CDS YP_667024.1 110670467 4199123 904641..905216 1 NC_008245.1 Similar to Q8DBC3 Folate-dependent phosphoribosylglycinamide formyltransferase from Vibrio vulnificus (212 aa). FASTA: opt: 530 Z-score: 633.0 E(): 2.1e-27 Smith-Waterman score: 530; 45.263 identity in 190 aa overlap.; phosphoribosylglycinamide formyltransferase 905216 purN 4199123 purN Francisella tularensis subsp. tularensis FSC198 phosphoribosylglycinamide formyltransferase YP_667024.1 904641 D 393115 CDS YP_667025.1 110670468 4199117 905333..905824 1 NC_008245.1 Similar to P72157 Phosphoribosylaminoimidazole carboxylase catalytic subunit from Psudomonas aeruginosa (163 aa). FASTA: opt: 719 Z-score: 894.1 E(): 6.6e-42 Smith-Waterman score: 719; 70.988 identity n 162 aa overlap; phosphoribosylaminoimidazole carboxylase,catalyic subunit 905824 purE 4199117 purE Francisella tularensis subsp. tularensis FSC198 phosphoribosylaminoimidazole carboxylase,catalyic subunit YP_667025.1 905333 D 393115 CDS YP_667026.1 110670469 4199120 905828..906925 1 NC_008245.1 With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway; phosphoribosylaminoimidazole carboxylase ATPase subunit 906925 purK 4199120 purK Francisella tularensis subsp. tularensis FSC198 phosphoribosylaminoimidazole carboxylase ATPase subunit YP_667026.1 905828 D 393115 CDS YP_667027.1 110670470 4199366 complement(906922..907275) 1 NC_008245.1 Similar to Q88N88 Conserved hypothetical protein TIGR002 from Pseudomonas putida (124 aa). FASTA: opt: 291 Z-score: 402.6 E(): 1.6e-14 Smith-Waterman score: 291; 38.938 identity in 113 aa overlap. ORF ftt0898c; hypothetical protein 907275 4199366 FTF0898c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667027.1 906922 R 393115 CDS YP_667028.1 110670471 4199941 complement(907342..909363) 1 NC_008245.1 Similar to P27298 Oligopeptidase A from Escherichia coli (680 aa). FASTA: opt: 1936 Z-score: 2040.0 E(): 8.9e-106 Smith-Waterman score: 1936; 43.722 identity in 677 aa overlap.; oligopeptidase A 909363 prlC 4199941 prlC Francisella tularensis subsp. tularensis FSC198 oligopeptidase A YP_667028.1 907342 R 393115 CDS YP_667029.1 110670472 4199367 909455..909826 1 NC_008245.1 ORF ftt0900; hypothetical protein 909826 4199367 FTF0900 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667029.1 909455 D 393115 CDS YP_667030.1 110670473 4199567 909907..910356 1 NC_008245.1 Paralog to conserved hypothetical lipoprotein lpnB.; lipoprotein 910356 lpnA 4199567 lpnA Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667030.1 909907 D 393115 CDS YP_667031.1 110670474 4199368 910515..910994 1 NC_008245.1 paralog of FTF0903 ORF ftt0902; hypothetical protein 910994 4199368 FTF0902 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667031.1 910515 D 393115 CDS YP_667032.1 110670475 4199369 911094..911597 1 NC_008245.1 paralog of FTF0902 ORF ftt0903; hypothetical protein 911597 4199369 FTF0903 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667032.1 911094 D 393115 CDS YP_667033.1 110670476 4199568 911630..912097 1 NC_008245.1 Similar to P18149 17 kDa major membrane protein precursor from Francisella tularensis ssp. holarctica strain LVS (149 aa). FASTA: opt: 274 Z-score: 359.1 E(): 4.1e-12 Smith-Waterman score: 274; 32.468 identity (33.5570ngapped) in 154 aa overlap. Paralog to conserved hypothetical lipoprotein lpnA.; lipoprotein 912097 lpnB 4199568 lpnB Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667033.1 911630 D 393115 CDS YP_667034.1 110670477 4199370 912170..913612 1 NC_008245.1 Similar to Q8KQ19 PilO from Pseudomonas aeruginosa (461 aa). FASTA: opt: 283 Z-score: 329.7 E(): 1.6e-10 Smith-Waterman score: 283; 25.284 identity in 352 aa overlap. PilO is required for glycosylation of Pseudomonas aeruginosa pilin. ORF ftt0905; Type IV pili glycosylation protein 913612 4199370 FTF0905 Francisella tularensis subsp. tularensis FSC198 Type IV pili glycosylation protein YP_667034.1 912170 D 393115 CDS YP_667035.1 110670478 4199858 complement(913666..915960) 1 NC_008245.1 catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining; maintains net negative superhelicity; DNA topoisomerase I 915960 topA 4199858 topA Francisella tularensis subsp. tularensis FSC198 DNA topoisomerase I YP_667035.1 913666 R 393115 CDS YP_667036.1 110670479 4200093 916159..916800 1 NC_008245.1 Similar to Q9F260 Partition protein from Actinobacillus actinomycetemcomitans (213 aa). FASTA: opt: 286 Z-score: 339.2 E(): 4.8e-11 mith-Waterman score: 286; 29.245 identity in 212 aa overlap; chmomosome partition protein A 916800 parA 4200093 parA Francisella tularensis subsp. tularensis FSC198 chmomosome partition protein A YP_667036.1 916159 D 393115 CDS YP_667037.1 110670480 4200094 916793..917707 1 NC_008245.1 Similar to Q9CJA4 Chromosome partitioning protein from Lactococcus lactis (261 aa). FASTA: opt: 399 Z-score: 442.0 E(): 9e-17 Smith-Waterman score: 399; 33.333 identity in 264 aa overlap; chromosome partition protein B 917707 parB 4200094 parB Francisella tularensis subsp. tularensis FSC198 chromosome partition protein B YP_667037.1 916793 D 393115 CDS YP_667038.1 110670481 4199371 917721..918428 1 NC_008245.1 Similar to Q88LT5 Glutamine amidotransferase, class I from Pseudomonas putida (244 aa). FASTA: opt: 589 Z-score: 771.5 bits: 150.1 E(): 4.4e-35 Smith-Waterman score: 589; 36.325identity in 234 aa overlap. ORF ftt0909; glutamine amidotransferase 918428 4199371 FTF0909 Francisella tularensis subsp. tularensis FSC198 glutamine amidotransferase YP_667038.1 917721 D 393115 CDS YP_667039.1 110670482 4199372 918431..919312 1 NC_008245.1 Weakly similar to Q8GNM7 Lysophospholipase A from Legionella pneumophila (309 aa). FASTA: 86 opt: 302 Z-score: 378.8 bits: 78.1 E(): 3.3e-13 Smith-Waterman score: 360; 29.795 identity in 292 aa overlap. ORF ftt0910; hypothetical protein 919312 4199372 FTF0910 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667039.1 918431 D 393115 CDS YP_667040.1 110670483 4199373 919309..919899 1 NC_008245.1 Similar with Q87NK1 Hypothetical protein from Vibrio parahaemolyticus (195 aa) fasta scores; opt: 523 Z-score: 649.0 E(): 2.7e-28 Smith-Waterman score: 523; 42.857 identity in 189 aa overlap ORF ftt0911; hypothetical protein 919899 4199373 FTF0911 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667040.1 919309 D 393115 CDS YP_667041.1 110670484 4198962 complement(919959..920579) 1 NC_008245.1 Similar to Q8EHM3 Ribosomal RNA large subunit methyltransferase J from Shewanella oneidensis (209 aa). FASTA: opt: 730 Z-score: 844.0 E(): 3.7e-39 Smith-Waterman score: 730; 53.202identity in 203 aa overlap; ribosomal large subunit methyltransferase J 920579 rrmJ 4198962 rrmJ Francisella tularensis subsp. tularensis FSC198 ribosomal large subunit methyltransferase J YP_667041.1 919959 R 393115 CDS YP_667042.1 110670485 4199374 920737..921204 1 NC_008245.1 ORF ftt0913; hypothetical protein 921204 4199374 FTF0913 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667042.1 920737 D 393115 CDS YP_667043.1 110670486 4199651 complement(921199..921684) 1 NC_008245.1 lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis; lipoprotein signal peptidase 921684 lspA 4199651 lspA Francisella tularensis subsp. tularensis FSC198 lipoprotein signal peptidase YP_667043.1 921199 R 393115 CDS YP_667044.1 110670487 4200661 complement(921671..924478) 1 NC_008245.1 IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme; isoleucyl-tRNA synthetase 924478 ileS 4200661 ileS Francisella tularensis subsp. tularensis FSC198 isoleucyl-tRNA synthetase YP_667044.1 921671 R 393115 CDS YP_667045.1 110670488 4199216 complement(924571..925491) 1 NC_008245.1 Similar to Q9CKF8 RibF from Pasteurella multocida (311 aa). FASTA: opt: 845 Z-score: 994.4 E(): 1.5e-47 Smith-Waterman score: 845; 44.481 identity in 308 aa overlap; riboflavin biosynthesis protein RibF 925491 ribF 4199216 ribF Francisella tularensis subsp. tularensis FSC198 riboflavin biosynthesis protein RibF YP_667045.1 924571 R 393115 CDS YP_667046.1 110670489 4199656 925642..927456 1 NC_008245.1 malic enzyme; oxaloacetate-decarboxylating; NAD-dependent; catalyzes the formation of pyruvate form malate; malate dehydrogenase 927456 maeA 4199656 maeA Francisella tularensis subsp. tularensis FSC198 malate dehydrogenase YP_667046.1 925642 D 393115 CDS YP_667047.1 110670490 4199375 927619..929292 1 NC_008245.1 ORF ftt0918; hypothetical protein 929292 4199375 FTF0918 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667047.1 927619 D 393115 CDS YP_667048.1 110670491 4199376 929309..930754 1 NC_008245.1 ORF ftt0919; hypothetical protein 930754 4199376 FTF0919 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667048.1 929309 D 393115 CDS YP_667049.1 110670492 4199637 join(930889..931215,931215..931673) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 931673 isftu1 4199637 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667049.1 930889 D 393115 CDS YP_667050.1 110670493 4199378 932841..933395 1 NC_008245.1 Similarity to Q98JM3 Probable fatty acid hydroxylase from Rhizobium loti (164 aa). FASTA: opt: 305 Z-score: 383.8 E(): 1.6e-13 Smith-Waterman score: 305; 31.902 identity in 163 aa overlap. ORF ftt0922; fatty acid hydroxylase 933395 4199378 FTF0922 Francisella tularensis subsp. tularensis FSC198 fatty acid hydroxylase YP_667050.1 932841 D 393115 CDS YP_667051.1 110670494 4199379 933470..935623 1 NC_008245.1 catalyzes the N2-methyl guanosine modification of the G2445 residue of 23S rRNA; 23S rRNA m(2)G2445 methyltransferase 935623 rlmL 4199379 rlmL Francisella tularensis subsp. tularensis FSC198 23S rRNA m(2)G2445 methyltransferase YP_667051.1 933470 D 393115 CDS YP_667052.1 110670495 4199380 935725..936123 1 NC_008245.1 ORF ftt0924; hypothetical protein 936123 4199380 FTF0924 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667052.1 935725 D 393115 CDS YP_667053.1 110670496 4199412 936128..937069 1 NC_008245.1 Similarity to P44787 Methionyl-tRNA formyltransferase from Haemophilus ducreyi (318 aa). FASTA: opt: 949 Z-score: 1086.2 E(): 1.2e-52 Smith-Waterman score: 958; 48.387 identity in 310 aa overlap; methionyl-tRNA formyltransferase 937069 fmt 4199412 fmt Francisella tularensis subsp. tularensis FSC198 methionyl-tRNA formyltransferase YP_667053.1 936128 D 393115 CDS YP_667054.1 110670497 4200363 937073..938047 1 NC_008245.1 catalyzes the second step in the glutathione biosynthesis pathway, where it synthesizes ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione; glutathione synthetase 938047 gshB 4200363 gshB Francisella tularensis subsp. tularensis FSC198 glutathione synthetase YP_667054.1 937073 D 393115 CDS YP_667055.1 110670498 4200378 938037..939332 1 NC_008245.1 Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway; glutamate-1-semialdehyde aminotransferase 939332 hemL 4200378 hemL Francisella tularensis subsp. tularensis FSC198 glutamate-1-semialdehyde aminotransferase YP_667055.1 938037 D 393115 CDS YP_667056.1 110670499 4199381 complement(939353..941020) 1 NC_008245.1 SImilar to Q8RD12 Beta-glucosidase-related glycosidases from Thermoanaerobacter tengcongensis (591 aa). FASTA: opt: 1047 Z-score: 1141.5 E(): 9.9e-56 Smith-Waterman score: 1062; 35.106 identity in 564 aa overlap. ORF ftt0928c; beta-glucosidase 941020 4199381 FTF0928c Francisella tularensis subsp. tularensis FSC198 beta-glucosidase YP_667056.1 939353 R 393115 CDS YP_667057.1 110670500 4200027 942762..944021 1 NC_008245.1 Similar to Q89YS8 Glucose/galactose transporter from Bacteroides thetaiotaomicron (415 aa). FASTA: opt: 1125 Z-score: 1176.3 bits: 226.6 E(): 1.3e-57 Smith-Waterman score: 1125; 41.446 identity in 415 aa overlap ORF ftt0931; major facilitator transporter 944021 4200027 FTF0931 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667057.1 942762 D 393115 CDS YP_667058.1 110670501 4200028 944056..944907 1 NC_008245.1 Similar to Q8A1Q9 ROK family transcriptional repressor from Bacteroides thetaiotaomicron (298 aa). FASTA: opt: 482 Z-score: 605.6 E(): 7.7e-26 Smith-Waterman score: 482; 30.769 identity in 286 aa overlap ORF ftt0932; ROK family protein 944907 4200028 FTF0932 Francisella tularensis subsp. tularensis FSC198 ROK family protein YP_667058.1 944056 D 393115 CDS YP_667059.1 110670502 4200306 complement(945833..946510) 1 NC_008245.1 Similar to AAO90524 Dethiobiotin synthetase from Coxiella burnetii (242 aa). FASTA: opt: 464 Z-score: 551.2 E(): 7.5e-23 Smith-Waterman score: 464; 34.498 identity in 229 aa overlap; dethiobiotin synthetase 946510 bioD 4200306 bioD Francisella tularensis subsp. tularensis FSC198 dethiobiotin synthetase YP_667059.1 945833 R 393115 CDS YP_667060.1 110670503 4200305 complement(946514..947251) 1 NC_008245.1 Similar to AAO90525 Biotin synthesis protein BioC,putative from Coxiella burnetii (248 aa) FASTA: opt: 232 Z-score: 273.5 E(): 2.2e-07 Smith-Waterman score: 232; 23.293 identity in 249 aa overlap; biotin synthesis protein BioC 947251 bioC 4200305 bioC Francisella tularensis subsp. tularensis FSC198 biotin synthesis protein BioC YP_667060.1 946514 R 393115 CDS YP_667061.1 110670504 4200307 complement(947229..948356) 1 NC_008245.1 Similar to AAO90527 8-amino-7-oxononanoate synthase (384 aa) fasta scores; opt: 763 Z-score: 912.3 E(): 5.8e-43 Smith-Waterman score: 763; 39.053identity in 338 aa overlap; 8-amino-7-oxononanoate synthase 948356 bioF 4200307 bioF Francisella tularensis subsp. tularensis FSC198 8-amino-7-oxononanoate synthase YP_667061.1 947229 R 393115 CDS YP_667062.1 110670505 4199704 complement(948350..949291) 1 NC_008245.1 Similar to AAO90528 Biotin synthase from Coxiella burnetii (321 aa) FASTA: opt: 1220 Z-score: 1430.1 E(): 8.3e-72 Smith-Waterman score: 1220; 60.396 identity in 303 aa overlap; biotin synthase 949291 bioB 4199704 bioB Francisella tularensis subsp. tularensis FSC198 biotin synthase YP_667062.1 948350 R 393115 CDS YP_667063.1 110670506 4199703 949337..950668 1 NC_008245.1 Similar to AAO90529 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase from Coxiella burnetii (442 aa). FASTA: opt: 1486 Z-score: 1733.9 E(): 9.9e-89 Smith-Waterman score: 1486; 50.588 identity in 425 aa overlap; adenosylmethionine-8-amino-7-oxononanoate aminotransferase 950668 bioA 4199703 bioA Francisella tularensis subsp. tularensis FSC198 adenosylmethionine-8-amino-7-oxononanoate aminotransferase YP_667063.1 949337 D 393115 CDS YP_667064.1 110670507 4199756 complement(950778..951722) 1 NC_008245.1 catalyzes the formation of inosine from adenosine; adenosine deaminase 951722 add1 4199756 add1 Francisella tularensis subsp. tularensis FSC198 adenosine deaminase YP_667064.1 950778 R 393115 CDS YP_667065.1 110670508 4200030 complement(951870..952370) 1 NC_008245.1 Similar to Q88CI3 Conserved hypothetical protein from Pseudomonas putida (160 aa). FASTA: opt: 297 Z-score: 384.8 E(): 1.4e-13 Smith-Waterman score: 297; 32.653 identity in 147 aa overlap ORF ftt0940c; hypothetical protein 952370 4200030 FTF0940c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667065.1 951870 R 393115 CDS YP_667066.1 110670509 4200031 complement(952386..953306) 1 NC_008245.1 Similar to Q97VW1 Lipase (lipP-1) from Sulfolobus solfataricus (305 aa). FASTA: opt: 488 Z-score: 622.4 E(): 8.9e-27 Smith-Waterman score: 488; 32.990 identity in 291 aa overlap. ORF ftt0941c; lipase/esterase 953306 4200031 FTF0941c Francisella tularensis subsp. tularensis FSC198 lipase/esterase YP_667066.1 952386 R 393115 CDS YP_667067.1 110670510 4199418 complement(953319..954584) 1 NC_008245.1 Similar to Q821E2 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase from Chlamydophila caviae (447 aa). FASTA: opt: 517 Z-score: 602.2 E(): 1.2e-25 Smith-Waterman score: 604; 30.664 identity in 437 aa overlap; 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase 954584 folK 4199418 folK Francisella tularensis subsp. tularensis FSC198 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase YP_667067.1 953319 R 393115 CDS YP_667068.1 110670511 4199414 complement(954586..954939) 1 NC_008245.1 Similar to O84620 Probable dihydroneopterin aldolase (124 aa) fasta scores; opt: 191 Z-score: 262.4 E(): 9.2e-07 Smith-Waterman score: 191; 30.189identity in 106 aa overlap; dihydroneopterin aldolase 954939 folB 4199414 folB Francisella tularensis subsp. tularensis FSC198 dihydroneopterin aldolase YP_667068.1 954586 R 393115 CDS YP_667069.1 110670512 4200032 955009..956391 1 NC_008245.1 Similar to Q9CHK3 O-succinylbenzoic acid-CoA ligase from Lactococcus lactis (420 aa). opt: 373 Z-score: 423.0 E(): 1e-15 Smith-Waterman score: 434; 27.692 identity in 390 aa overlap ORF ftt0944; AMP-binding family protein 956391 4200032 FTF0944 Francisella tularensis subsp. tularensis FSC198 AMP-binding family protein YP_667069.1 955009 D 393115 CDS YP_667070.1 110670513 4200033 956384..958147 1 NC_008245.1 Similar to Q8ESD8 Para-aminobenzoate synthase component II (585 aa) fasta scores; opt: 1108 Z-score: 1235.5 E(): 5.7e-61 Smith-Waterman score: 1108; 33.851 identity in 579 aa overlap. ORF ftt0945; chorismate binding family protein 958147 4200033 FTF0945 Francisella tularensis subsp. tularensis FSC198 chorismate binding family protein YP_667070.1 956384 D 393115 CDS YP_667071.1 110670514 4200720 958149..958703 1 NC_008245.1 Similarity to Q972A0 Putative anthranilate synthase component from Sulfolobus tokodaii (193 aa). FASTA: opt: 499 Z-score: 620.3 E(): 1.1e-26 Smith-Waterman score: 499; 40.741 identity in 189 aa overlap. Closely related to pabA proteins as they possess a shared domain feature; anthranilate synthase component II 958703 trpG1 4200720 trpG1 Francisella tularensis subsp. tularensis FSC198 anthranilate synthase component II YP_667071.1 958149 D 393115 CDS YP_667072.1 110670515 4200035 complement(959924..960988) 1 NC_008245.1 Similar to Q92QZ5 Putative oxidoreductase protein from Rhizobium meliloti (347 aa). FASTA: opt: 1125 Z-score: 1304.5 E(): 8.2e-65 Smith-Waterman score: 1125; 50.140 identity in 357 aa overlap. putative pseudogene: flanked by pseudogenes ORF ftt0948c; aldo/keto reductase 960988 4200035 FTF0948c Francisella tularensis subsp. tularensis FSC198 aldo/keto reductase YP_667072.1 959924 R 393115 CDS YP_667073.1 110670516 4199417 complement(963351..963971) 1 NC_008245.1 involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer; GTP cyclohydrolase I 963971 folE 4199417 folE Francisella tularensis subsp. tularensis FSC198 GTP cyclohydrolase I YP_667073.1 963351 R 393115 CDS YP_667074.1 110670517 4199210 964098..965423 1 NC_008245.1 Similar to Q8D6C6 Superfamily II DNA and RNA helicase from Vibrio vulnificus (509 aa). FASTA: opt: 1533 Z-score: 1649.9 E(): 4.8e-84 Smith-Waterman score: 1533; 56.036 identity in 439 aa overlap; ATP-dependent RNA helicase RhlE 965423 rhlE 4199210 rhlE Francisella tularensis subsp. tularensis FSC198 ATP-dependent RNA helicase RhlE YP_667074.1 964098 D 393115 CDS YP_667075.1 110670518 4200038 complement(965420..966961) 1 NC_008245.1 Similar to Q9KTB5 Proton/peptide symporter family protein from Vibrio cholerae (514 aa). FASTA: opt: 1329 Z-score: 1443.3 E(): 1.5e-72 Smith-Waterman score: 1329; 42.629identity in 502 aa overlap. ORF ftt0953c; proton-dependent oligopeptide transport (POT) family protein 966961 4200038 FTF0953c Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_667075.1 965420 R 393115 CDS YP_667076.1 110670519 4200039 complement(967099..967419) 1 NC_008245.1 Similar to O29392 Hypothetical protein AF0869 from Archaeoglobus fulgidus (108 aa). FASTA: opt: 272 Z-score: 377.5 E(): 3.9e-13 Smith-Waterman score: 272; 42.056 identity in 107 aa overlap ORF ftt0954c; hypothetical protein 967419 4200039 FTF0954c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667076.1 967099 R 393115 CDS YP_667077.1 110670520 4200292 complement(967430..968791) 1 NC_008245.1 catalyzes the reduction of 2 glutathione to glutathione disulfide; maintains high levels of reduced glutathione in the cytosol; involved in redox regulation and oxidative defense; glutathione reductase 968791 gor 4200292 gor Francisella tularensis subsp. tularensis FSC198 glutathione reductase YP_667077.1 967430 R 393115 CDS YP_667078.1 110670521 4200040 complement(968829..969548) 1 NC_008245.1 Similar to AAO50751 (Q86L42) Hypothetical protein from Dictyostelium discoideum (239 aa). FASTA: opt: 229 Z-score: 273.3 E(): 2.3e-07 Smith-Waterman score: 253; 29.437 identity in 231 aa overlap ORF ftt0956c; hypothetical protein 969548 4200040 FTF0956c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667078.1 968829 R 393115 CDS YP_667079.1 110670522 4200041 complement(969548..970051) 1 NC_008245.1 Similar to Q83YE2 Shy8 from Streptomyces hygroscopicus (163 aa). FASTA: opt: 343 Z-score: 413.4 bits: 82.8 E(): 3.9e-15 Smith-Waterman score: 343; 36.420 identity in 162 aa overlap. ORF ftt0957c; hypothetical protein 970051 4200041 FTF0957c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667079.1 969548 R 393115 CDS YP_667080.1 110670523 4200042 complement(970054..970761) 1 NC_008245.1 Similar to Q9HWL9 Probable short-chain dehydrogenase from Pseudomonas aeruginosa (238 aa). FASTA: opt: 848 Z-score: 1032.8 E(): 1.1e-49 Smith-Waterman score: 848; 55.932 identity in 236 aa overlap ORF ftt0958c; short chain dehydrogenase 970761 4200042 FTF0958c Francisella tularensis subsp. tularensis FSC198 short chain dehydrogenase YP_667080.1 970054 R 393115 CDS YP_667081.1 110670524 4200696 complement(970782..971570) 1 NC_008245.1 Similar to Q83EM1 Exodeoxyribonuclease III from Coxiella burnetii (259 aa). FASTA: opt: 991 Z-score: 1173.5 E(): 1.6e-57 Smith-Waterman score: 991; 54.789 identity in 261 aa overlap; exodeoxyribonuclease III 971570 xthA 4200696 xthA Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease III YP_667081.1 970782 R 393115 CDS YP_667082.1 110670525 4200043 971854..972837 1 NC_008245.1 Simiar to Q8EAC2 Conserved hypothetical protein Shewanella oneidensis (371 aa). FASTA: opt: 607 Z-score: 717.9 E(): 3.9e-32 Smith-Waterman score: 607; 32.622 identity in 328 aa overlap ORF ftt0960; hypothetical protein 972837 4200043 FTF0960 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667082.1 971854 D 393115 CDS YP_667083.1 110670526 4199662 972845..973435 1 NC_008245.1 Similar to P40717 Modulator of drug activity B from Escherichia coli (193 aa). FASTA: opt: 900 Z-score: 1129.6 E(): 4.5e-55 Smith-Waterman score: 900; 63.542 identity in 192 aa overlap.; modulator of drug activity B 973435 mdaB 4199662 mdaB Francisella tularensis subsp. tularensis FSC198 modulator of drug activity B YP_667083.1 972845 D 393115 CDS YP_667084.1 110670527 4199323 973551..974213 1 NC_008245.1 Similar to Q87Z85 ThiJ/PfpI family protein from Pseudomonas syringae (225 aa). FASTA: opt: 570 Z-score: 665.3 E(): 3.3e-29 Smith-Waterman score: 570; 38.991 identity in 218 aa overlap ORF ftt0962; ThiJ/PfpI family protein 974213 4199323 FTF0962 Francisella tularensis subsp. tularensis FSC198 ThiJ/PfpI family protein YP_667084.1 973551 D 393115 CDS YP_667085.1 110670528 4199437 complement(974283..975395) 1 NC_008245.1 catalyzes the formation of 3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate, phenylalanine sensitive; phospho-2-dehydro-3-deoxyheptonate aldolase 975395 aroG 4199437 aroG Francisella tularensis subsp. tularensis FSC198 phospho-2-dehydro-3-deoxyheptonate aldolase YP_667085.1 974283 R 393115 CDS YP_667086.1 110670529 4199410 complement(975427..976803) 1 NC_008245.1 Similar to Q9CLN6 Ffh from Pasteurella multocida (458 aa). FASTA: opt: 1851 Z-score: 1789.9 E(): 7.5e-92 Smith-Waterman score: 1851; 61.790 identity in 458 aa overlap; signal recognition particle protein, Ffh 976803 ffh 4199410 ffh Francisella tularensis subsp. tularensis FSC198 signal recognition particle protein, Ffh YP_667086.1 975427 R 393115 CDS YP_667087.1 110670530 4199324 complement(977147..978052) 1 NC_008245.1 Similar to Q928M9 Hypothetical protein lin2503 from Listeria innocua (279 aa). FASTA: opt: 733 Z-score: 821.7 E(): 6.4e-38 Smith-Waterman score: 733; 41.852 identity in 270 aa overlap. ORF ftt0965c; hypothetical protein 978052 4199324 FTF0965c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667087.1 977147 R 393115 CDS YP_667088.1 110670531 4199609 978146..978364 1 NC_008245.1 stimulates the activities of the other two initiation factors, IF-2 and IF-3; translation initiation factor IF-1 978364 infA 4199609 infA Francisella tularensis subsp. tularensis FSC198 translation initiation factor IF-1 YP_667088.1 978146 D 393115 CDS YP_667089.1 110670532 4199326 complement(979326..980741) 1 NC_008245.1 Similar to Q83BZ0 Amino acid antiporter from Coxiella burnetii (474 aa). FASTA: 432 opt: 1275 Z-score: 1361.2 E(): 6.3e-68 Smith-Waterman score: 1275; 40.171 identity in 468 aa overlap. ORF ftt0968c; amino acid antiporter 980741 4199326 FTF0968c Francisella tularensis subsp. tularensis FSC198 amino acid antiporter YP_667089.1 979326 R 393115 CDS YP_667090.1 110670533 4199861 complement(980806..982179) 1 NC_008245.1 involved in potassium uptake; found to be peripherally associated with the inner membrane in Escherichia coli; contains an NAD-binding domain; potassium transporter peripheral membrane protein 982179 trkA 4199861 trkA Francisella tularensis subsp. tularensis FSC198 potassium transporter peripheral membrane protein YP_667090.1 980806 R 393115 CDS YP_667091.1 110670534 4199327 982332..982742 1 NC_008245.1 Similar to Q8PIG4 Hypothetical protein XAC2934 from Xanthomonas axonopodis (152 aa). FASTA: opt: 281 Z-score: 360.3 E(): 3.2e-12 Smith-Waterman score: 281; 33.588 identity in 131 aa overlap ORF ftt0970; hypothetical protein 982742 4199327 FTF0970 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667091.1 982332 D 393115 CDS YP_667092.1 110670535 4199328 982749..984194 1 NC_008245.1 with SufCD activates cysteine desulfurase SufS; cysteine desulfurase activator complex subunit SufB 984194 4199328 FTF0971 Francisella tularensis subsp. tularensis FSC198 cysteine desulfurase activator complex subunit SufB YP_667092.1 982749 D 393115 CDS YP_667093.1 110670536 4199329 984233..984982 1 NC_008245.1 Similar to Q8PIG2 ABC transporter ATP-binding protein from Xanthomonas axonopodis (254 aa). FASTA: opt: 1080 Z-score: 1117.5 E(): 2.1e-54 Smith-Waterman score: 1080; 65.041 identity in 246 aa overlap. ORF ftt0972; ABC transporter ATP-binding protein 984982 4199329 FTF0972 Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_667093.1 984233 D 393115 CDS YP_667094.1 110670537 4199330 984986..986140 1 NC_008245.1 Similar to Q83BX9 Hypothetical protein CBU1358 Coxiella burnetii (426 aa). FASTA: opt: 433 Z-score: 493.2 E(): 1.3e-19 Smith-Waterman score: 460; 29.282 identity in 362 aa overlap ORF ftt0973; hypothetical protein 986140 4199330 FTF0973 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667094.1 984986 D 393115 CDS YP_667095.1 110670538 4199332 987109..987798 1 NC_008245.1 Weakly similar to Q83CZ8 Hypothetical protein Coxiella burnetii (227 aa) 76 opt: 235 Z-score: 276.0 bits: 58.3 E(): 1.8e-07 Smith-Waterman score: 235; 25.000 identity in 236 aa overlap. ORF ftt0975; hypothetical protein 987798 4199332 FTF0975 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667095.1 987109 D 393115 CDS YP_667096.1 110670539 4198970 987802..988128 1 NC_008245.1 Similar to P37395 Thioredoxin from Cyanidium caldarium (107 aa) fasta scores; opt: 333 Z-score: 452.0 E(): 2.5e-17 Smith-Waterman score: 333; 45.098identity in 102 aa overlap; thioredoxin 988128 trxA2 4198970 trxA2 Francisella tularensis subsp. tularensis FSC198 thioredoxin YP_667096.1 987802 D 393115 CDS YP_667097.1 110670540 4199333 complement(988527..988820) 1 NC_008245.1 Similar to Q89DD5 Bsl7504 protein from Bradyrhizobium japonicum (99 aa). FASTA: opt: 209 Z-score: 292.0 E(): 2e-08 Smith-Waterman score: 209; 38.462 identity in 91 aa overlap ORF ftt0977c; hypothetical protein 988820 4199333 FTF0977c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667097.1 988527 R 393115 CDS YP_667098.1 110670541 4199334 complement(988824..989429) 1 NC_008245.1 ORF ftt0978c; hypothetical protein 989429 4199334 FTF0978c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667098.1 988824 R 393115 CDS YP_667099.1 110670542 4199335 complement(989483..990850) 1 NC_008245.1 amino-acid permease, similarity to P39137 Amino-acid permease rocE from Bacillus subtilis (467 aa). FASTA: opt: 1272 Z-score: 1431.9 E(): 6.6e-72 Smith-Waterman score: 1272; 40.919 identity in 457 aa overlap. ORF ftt0979c; amino-acid permease 990850 4199335 FTF0979c Francisella tularensis subsp. tularensis FSC198 amino-acid permease YP_667099.1 989483 R 393115 CDS YP_667100.1 110670543 4199336 991085..992326 1 NC_008245.1 Similar to Q8UKI4 Aminotransferase, class II from Agrobacterium tumefaciens (413 aa). FASTA: opt: 486 Z-score: 559.2 E(): 2.7e-23 Smith-Waterman score: 554; 28.493 identity in 365 aa overlap ORF ftt0980; hypothetical protein 992326 4199336 FTF0980 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667100.1 991085 D 393115 CDS YP_667101.1 110670544 4199337 992659..993789 1 NC_008245.1 Weak homology to two-component sensory protein. ORF ftt0981; hypothetical protein 993789 4199337 FTF0981 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667101.1 992659 D 393115 CDS YP_667102.1 110670545 4199338 993882..994025 1 NC_008245.1 ORF ftt0982; hypothetical protein 994025 4199338 FTF0982 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667102.1 993882 D 393115 CDS YP_667103.1 110670546 4199339 994009..994152 1 NC_008245.1 ORF ftt0983; hypothetical protein 994152 4199339 FTF0983 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667103.1 994009 D 393115 CDS YP_667104.1 110670547 4200115 complement(994739..995149) 1 NC_008245.1 Similar to Q87SF6 Mutator MutT protein from Vibrio parahaemolyticus (132 aa). FASTA: opt: 268 Z-score: 340.9 E(): 3.9e-11 Smith-Waterman score: 268; 29.545identity in 132 aa overlap.; Mutator protein 995149 mutT 4200115 mutT Francisella tularensis subsp. tularensis FSC198 Mutator protein YP_667104.1 994739 R 393115 CDS YP_667105.1 110670548 4199340 995188..995772 1 NC_008245.1 Similar to Q8EBZ9 Conserved hypothetical protein from Shewanella oneidensis (187 aa). FASTA: opt: 386 Z-score: 477.0 E(): 1e-18 Smith-Waterman score: 386; 36.022 identity in 186 aa overlap ORF ftt0985; hypothetical protein 995772 4199340 FTF0985 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667105.1 995188 D 393115 CDS YP_667106.1 110670549 4199341 995774..996184 1 NC_008245.1 Similar to Q9L3I7 Putative Holliday junction resolvase from Aeromonas hydrophila (140 aa). FASTA: opt: 336 Z-score: 430.0 E(): 4.7e-16 Smith-Waterman score: 336; 36.567 identity in 134 aa overlap Putative Holliday junction resolvase predicted in other proteins ORF ftt0986; hypothetical protein 996184 4199341 FTF0986 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667106.1 995774 D 393115 CDS YP_667107.1 110670550 4199342 996277..996474 1 NC_008245.1 ORF ftt0987; hypothetical protein 996474 4199342 FTF0987 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667107.1 996277 D 393115 CDS YP_667108.1 110670551 4199305 996597..996785 1 NC_008245.1 ORF ftt0988; hypothetical protein 996785 4199305 FTF0988 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667108.1 996597 D 393115 CDS YP_667109.1 110670552 4199306 996879..998774 1 NC_008245.1 Similar to Q9I271 Hypothetical protein PA2044 from Pseudomonas aeruginosa (624 aa). FASTA: opt: 447 Z-score: 484.0 E(): 4.2e-19 Smith-Waterman score: 447; 22.203 identity in 572 aa overlap. ORF ftt0989; hypothetical protein 998774 4199306 FTF0989 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667109.1 996879 D 393115 CDS YP_667110.1 110670553 4199514 998882..1001323 1 NC_008245.1 leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase; leucyl-tRNA synthetase 1001323 leuS 4199514 leuS Francisella tularensis subsp. tularensis FSC198 leucyl-tRNA synthetase YP_667110.1 998882 D 393115 CDS YP_667111.1 110670554 4199307 1001331..1001906 1 NC_008245.1 ORF ftt0991; lipoprotein 1001906 4199307 FTF0991 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667111.1 1001331 D 393115 CDS YP_667112.1 110670555 4199308 1001967..1003157 1 NC_008245.1 Similar to Q8A7A2 Putative membrane protein from Bacteroides thetaiotaomicron (391 aa). FASTA: opt: 908 Z-score: 1062.3 E(): 2.6e-51 Smith-Waterman score: 908; 37.637 identity in 364 aa overlap. ORF ftt0992; hypothetical protein 1003157 4199308 FTF0992 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667112.1 1001967 D 393115 CDS YP_667113.1 110670556 4199527 complement(1003164..1003730) 1 NC_008245.1 Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis; deoxycytidine triphosphate deaminase 1003730 dcd 4199527 dcd Francisella tularensis subsp. tularensis FSC198 deoxycytidine triphosphate deaminase YP_667113.1 1003164 R 393115 CDS YP_667114.1 110670557 4199309 complement(1003742..1004602) 1 NC_008245.1 Similar to Q8XWU1 Probable MRP protein ATP-binding from Ralstonia solanacearum. (362 aa). FASTA: opt: 1008 Z-score: 1105.3 E(): 1e-53 Smith-Waterman score: 1008; 52.688 identity in 279 aa overlap. ORF ftt0994c; MRP like protein 1004602 4199309 FTF0994c Francisella tularensis subsp. tularensis FSC198 MRP like protein YP_667114.1 1003742 R 393115 CDS YP_667115.1 110670558 4199310 1004676..1005908 1 NC_008245.1 Similarity to Q8UDH1 MFS permease[proline/betaine] from Agrobacterium tumefaciens (443 aa). FASTA: opt: 727 Z-score: 824.0 E(): 4.8e-38 Smith-Waterman score: 828; 35.507identity in 414 aa overlap ORF ftt0995; major facilitator transporter 1005908 4199310 FTF0995 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667115.1 1004676 D 393115 CDS YP_667116.1 110670559 4200705 1007478..1008908 1 NC_008245.1 Similar to P71040 Probable cardiolipin synthetase 2 from Bacillus subtilis (482 aa). FASTA: opt: 835 Z-score: 944.7 E(): 9.1e-45 Smith-Waterman score: 835; 32.525 identity in 495 aa overlap; cardiolipin synthetase 1008908 ybhO 4200705 ybhO Francisella tularensis subsp. tularensis FSC198 cardiolipin synthetase YP_667116.1 1007478 D 393115 CDS YP_667117.1 110670560 4199312 1009074..1010063 1 NC_008245.1 ORF ftt0998; hypothetical protein 1010063 4199312 FTF0998 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667117.1 1009074 D 393115 CDS YP_667118.1 110670561 4200272 complement(1010831..1012057) 1 NC_008245.1 Similar to Q8FUU8 Cobalamin synthesis protein/P47K family protein from Brucella suis (400 aa). FASTA: opt: 1643 Z-score: 1956.7 E(): 4.3e-101 Smith-Waterman score: 1643; 58.396 identity in 399 aa overlap.; cobalamin (vitamin B12) synthesis protein/P47K family protein 1012057 cobS 4200272 cobS Francisella tularensis subsp. tularensis FSC198 cobalamin (vitamin B12) synthesis protein/P47K family protein YP_667118.1 1010831 R 393115 CDS YP_667119.1 110670562 4199314 1012199..1012630 1 NC_008245.1 Similar to Q8ZJ15 Putative regulatory protein from Yersinia pestis (169 aa). FASTA: opt: 313 Z-score: 406.8 E(): 9.2e-15 Smith-Waterman score: 313; 36.429 identity in 140 aa overlap. ORF ftt1001; regulatory protein 1012630 4199314 FTF1001 Francisella tularensis subsp. tularensis FSC198 regulatory protein YP_667119.1 1012199 D 393115 CDS YP_667120.1 110670563 4199725 complement(1012673..1015045) 1 NC_008245.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily; phenylalanyl-tRNA synthetase subunit beta 1015045 pheT 4199725 pheT Francisella tularensis subsp. tularensis FSC198 phenylalanyl-tRNA synthetase subunit beta YP_667120.1 1012673 R 393115 CDS YP_667121.1 110670564 4199724 complement(1015066..1016079) 1 NC_008245.1 catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily; phenylalanyl-tRNA synthetase subunit alpha 1016079 pheS 4199724 pheS Francisella tularensis subsp. tularensis FSC198 phenylalanyl-tRNA synthetase subunit alpha YP_667121.1 1015066 R 393115 CDS YP_667122.1 110670565 4199315 complement(1016165..1017058) 1 NC_008245.1 Similar to Q9RX03 Hypothetical protein DR0512 from Deinococcus radiodurans (301 aa). Similar to opt: 577 Z-score: 644.6 E(): 5.1e-28 Smith-Waterman score: 577; 33.094 identity in 278 aa overlap. ORF ftt1004c; hypothetical protein 1017058 4199315 FTF1004c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667122.1 1016165 R 393115 CDS YP_667123.1 110670566 4199106 complement(1017083..1018558) 1 NC_008245.1 Similar to Q8X5Q8 Putative transport protein from Escherichia coli (489 aa). FASTA: opt: 1050 Z-score: 1137.7 E(): 1.8e-55 Smith-Waterman score: 1050; 35.569 identity in 492 aa overlap.; proton-dependent oligopeptide transporter (POT) family protein 1018558 yhiP 4199106 yhiP Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transporter (POT) family protein YP_667123.1 1017083 R 393115 CDS YP_667124.1 110670567 4199316 1018802..1020010 1 NC_008245.1 ORF ftt1006; hypothetical protein 1020010 4199316 FTF1006 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667124.1 1018802 D 393115 CDS YP_667125.1 110670568 4199317 complement(1019995..1020717) 1 NC_008245.1 ORF ftt1007c; hypothetical protein 1020717 4199317 FTF1007c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667125.1 1019995 R 393115 CDS YP_667126.1 110670569 4199318 complement(1020778..1020942) 1 NC_008245.1 ORF ftt1008c; hypothetical protein 1020942 4199318 FTF1008c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667126.1 1020778 R 393115 CDS YP_667127.1 110670570 4199319 1021070..1021606 1 NC_008245.1 ORF ftt1009; hypothetical protein 1021606 4199319 FTF1009 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667127.1 1021070 D 393115 CDS YP_667128.1 110670571 4199320 1021621..1021824 1 NC_008245.1 Similar to Q81BZ8 Transcriptional regulator,Cro/CI family protein from Bacillus cereus (strain ATCC 14579 / DSM 31) (71 aa). FASTA: opt: 263 Z-score: 391.6 E(): 6.4e-14 Smith-Waterman score: 263; 60.606 identity in 66 aa overlap. ORF ftt1010; helix-turn-helix family protein 1021824 4199320 FTF1010 Francisella tularensis subsp. tularensis FSC198 helix-turn-helix family protein YP_667128.1 1021621 D 393115 CDS YP_667129.1 110670572 4199321 1021936..1022124 1 NC_008245.1 ORF ftt1011; hypothetical protein 1022124 4199321 FTF1011 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667129.1 1021936 D 393115 CDS YP_667130.1 110670573 4198966 complement(1023111..1024157) 1 NC_008245.1 promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration; Holliday junction DNA helicase RuvB 1024157 ruvB 4198966 ruvB Francisella tularensis subsp. tularensis FSC198 Holliday junction DNA helicase RuvB YP_667130.1 1023111 R 393115 CDS YP_667131.1 110670574 4200667 complement(1024221..1024946) 1 NC_008245.1 Similar to YWFD_BACSU (P39640) Hypothetical oxidoreductase ywfD from Bacillus subtilis (255 aa). FASTA: opt: 410 Z-score: 474.7 E(): 1.5e-18 Smith-Waterman score: 471; 38.710 identity in 248 aa overlap. ORF ftt1014c; short chain dehydrogenase family protein 1024946 4200667 FTF1014c Francisella tularensis subsp. tularensis FSC198 short chain dehydrogenase family protein YP_667131.1 1024221 R 393115 CDS YP_667132.1 110670575 4200668 1025064..1025654 1 NC_008245.1 ORF ftt1015; hypothetical protein 1025654 4200668 FTF1015 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667132.1 1025064 D 393115 CDS YP_667133.1 110670576 4200669 complement(1025664..1026293) 1 NC_008245.1 Similar to Q9JQY7 Putative lipase (GDSL lipase family protein) from Neisseria meningitidis (213 aa). FASTA: opt: 358 Z-score: 439.3 E(): 1.4e-16 Smith-Waterman score: 358; 32.203 identity in 177 aa overlap. ORF ftt1016c; GDSL-like lipase/acylhydrolase family protein 1026293 4200669 FTF1016c Francisella tularensis subsp. tularensis FSC198 GDSL-like lipase/acylhydrolase family protein YP_667133.1 1025664 R 393115 CDS YP_667134.1 110670577 4200670 complement(1026315..1026650) 1 NC_008245.1 ORF ftt1017c; hypothetical protein 1026650 4200670 FTF1017c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667134.1 1026315 R 393115 CDS YP_667135.1 110670578 4199668 complement(1026666..1028513) 1 NC_008245.1 catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate; 1-deoxy-D-xylulose-5-phosphate synthase 1028513 dxs 4199668 dxs Francisella tularensis subsp. tularensis FSC198 1-deoxy-D-xylulose-5-phosphate synthase YP_667135.1 1026666 R 393115 CDS YP_667136.1 110670579 4200365 complement(1028595..1030145) 1 NC_008245.1 contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway; GMP synthase 1030145 guaA 4200365 guaA Francisella tularensis subsp. tularensis FSC198 GMP synthase YP_667136.1 1028595 R 393115 CDS YP_667137.1 110670580 4200671 complement(1030233..1031780) 1 NC_008245.1 Similar to Q83EA0 Amino acid permease family protein from Coxiella burnetii (531 aa). FASTA: opt: 925 Z-score: 996.3 E(): 1.3e-47 Smith-Waterman score: 947; 29.379 identity in 531 aa overlap. ORF ftt1020c; amino acid permease 1031780 4200671 FTF1020c Francisella tularensis subsp. tularensis FSC198 amino acid permease YP_667137.1 1030233 R 393115 CDS YP_667138.1 110670581 4200724 complement(1031833..1032609) 1 NC_008245.1 mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability; tRNA pseudouridine synthase A 1032609 truA 4200724 truA Francisella tularensis subsp. tularensis FSC198 tRNA pseudouridine synthase A YP_667138.1 1031833 R 393115 CDS YP_667139.1 110670582 4200672 complement(1032612..1034444) 1 NC_008245.1 Similar to Y125_PYRHO (O57865) Hypothetical protein PH0125 from Pyrococcus horikoshii (257 aa) (from 1-260). FASTA: opt: 520 Z-score: 632.1 E(): 2.6e-27 Smith-Waterman score: 520; 35.600 identity in 250 aa overlap AND Q97VT8 Lipase (lipP-2) (EC 3.1.1.3) from Sulfolobus solfataricus (311 aa) (from 300-610). FASTA: opt: 613 Z-score: 743.7 E(): 1.6e-33 Smith-Waterman score: 613; 34.586 identity in 266 aa overlap Possible merged protein due to a missing stop codon between 2 CDSs? ORF ftt1022c; hypothetical protein 1034444 4200672 FTF1022c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667139.1 1032612 R 393115 CDS YP_667140.1 110670583 4200673 complement(1034457..1035050) 1 NC_008245.1 Similar to Q8EGL6 Fumarylacetoacetate hydrolase family protein from Shewanella oneidensis (208 aa). FASTA: opt: 599 Z-score: 746.5 E(): 1.1e-33 Smith-Waterman score: 599; 51.913 identity in 183 aa overlap. ORF ftt1023c; fumarylacetoacetate hydrolase family protein 1035050 4200673 FTF1023c Francisella tularensis subsp. tularensis FSC198 fumarylacetoacetate hydrolase family protein YP_667140.1 1034457 R 393115 CDS YP_667141.1 110670584 4199102 complement(1035099..1035830) 1 NC_008245.1 Similar to Q8ZB44 Probable ABC transporter ATP-binding protein from Yersinia pestis (241 aa). FASTA: opt: 1091 Z-score: 1233.5 E(): 8.2e-61 Smith-Waterman score: 1091; 65.690 identity in 239 aa overlap.; ABC transporter ATP-binding protein 1035830 yhbG 4199102 yhbG Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_667141.1 1035099 R 393115 CDS YP_667142.1 110670585 4200674 complement(1035904..1036737) 1 NC_008245.1 ORF ftt1025c; hypothetical protein 1036737 4200674 FTF1025c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667142.1 1035904 R 393115 CDS YP_667143.1 110670586 4200675 complement(1036724..1037350) 1 NC_008245.1 ORF ftt1026c; hypothetical protein 1037350 4200675 FTF1026c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667143.1 1036724 R 393115 CDS YP_667144.1 110670587 4199035 complement(1037358..1037906) 1 NC_008245.1 Similar to AAP18518 Hypothetical protein yrbI from Escherichia coli (188 aa). FASTA: initn: 454 init1: 247 opt: 453 Z-score: 558.6 bits: 110.0 E(): 3.2e-23 Smith-Waterman score: 453; 50.000 identity in 152 aa overlap.; 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase 1037906 yrbI 4199035 yrbI Francisella tularensis subsp. tularensis FSC198 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase YP_667144.1 1037358 R 393115 CDS YP_667145.1 110670588 4199932 complement(1037997..1038518) 1 NC_008245.1 Similar to IPYR_YERPE (Q8ZB98) Inorganic pyrophosphatase from Yersinia pestis (175 aa). FASTA: opt: 762 Z-score: 1001.4 E(): 6.9e-48 Smith-Waterman score: 762; 62.209 identity in 172 aa overlap.; inorganic pyrophosphatase 1038518 ppa 4199932 ppa Francisella tularensis subsp. tularensis FSC198 inorganic pyrophosphatase YP_667145.1 1037997 R 393115 CDS YP_667146.1 110670589 4199526 1038675..1040009 1 NC_008245.1 Similar to Q87VW4 D-alanyl-D-alanine carboxypeptidase from Pseudomonas syringae (pv. tomato) (386 aa). FASTA: opt: 602 Z-score: 704.0 E(): 2.5e-31 Smith-Waterman score: 794; 36.409 identity in 401 aa overlap.; D-alanyl-D-alanine carboxypeptidase 1040009 dacD 4199526 dacD Francisella tularensis subsp. tularensis FSC198 D-alanyl-D-alanine carboxypeptidase YP_667146.1 1038675 D 393115 CDS YP_667147.1 110670590 4200676 1040042..1040326 1 NC_008245.1 Similar to YE87_NITEU (Q82UJ7) Hypothetical UPF0250 protein NE1487 from Nitrosomonas europaea (87 aa). FASTA: opt: 235 Z-score: 328.5 E(): 2.1e-10 Smith-Waterman score: 235; 36.471 identity in 85 aa overlap. ORF ftt1030; hypothetical protein 1040326 4200676 FTF1030 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667147.1 1040042 D 393115 CDS YP_667148.1 110670591 4199555 1040336..1040956 1 NC_008245.1 lipoyl/octanoyltransferase; catalyzes the transfer of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto lipoate-dependent enzymes like pyruvate dehydrogenase and the glycine cleavage system H protein; lipoate-protein ligase B 1040956 lipB 4199555 lipB Francisella tularensis subsp. tularensis FSC198 lipoate-protein ligase B YP_667148.1 1040336 D 393115 CDS YP_667149.1 110670592 4200237 complement(1045025..1046287) 1 NC_008245.1 Similar to Q8UDU6 NADH dehydrogenase from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (438 aa). FASTA: opt: 826 Z-score: 983.7 E(): 6.7e-47 Smith-Waterman score: 826; 32.854 identity in 417 aa overlap.; NADH dehydrogenase 1046287 ndh 4200237 ndh Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase YP_667149.1 1045025 R 393115 CDS YP_667150.1 110670593 4200330 complement(1046394..1048127) 1 NC_008245.1 Similar to P77890 LprpoD (RNA polymerase sigma factor) from Legionella pneumophila (620 aa). FASTA: opt: 1367 Z-score: 1480.9 E(): 1.4e-74 Smith-Waterman score: 1624; 47.182 identity in 621 aa overlap.; RNA polymerase sigma-70 factor 1048127 rpoD 4200330 rpoD Francisella tularensis subsp. tularensis FSC198 RNA polymerase sigma-70 factor YP_667150.1 1046394 R 393115 CDS YP_667151.1 110670594 4199353 complement(1048147..1049964) 1 NC_008245.1 Similar to Q8DEG2 DNA primase from Vibrio vulnificus (587 aa). FASTA: opt: 1152 Z-score: 1324.3 E(): 7.2e-66 Smith-Waterman score: 1155; 39.679 identity in 499 aa overlap.; DNA primase 1049964 dnaG 4199353 dnaG Francisella tularensis subsp. tularensis FSC198 DNA primase YP_667151.1 1048147 R 393115 CDS YP_667152.1 110670595 4200678 complement(1049985..1050431) 1 NC_008245.1 Similar to Q87SL6 Conserved hypothetical protein from Vibrio parahaemolyticus (147 aa). FASTA: opt: 473 Z-score: 548.0 E(): 1.2e-22 Smith-Waterman score: 473; 47.586 identity in 145 aa overlap. ORF ftt1037c; hypothetical protein 1050431 4200678 FTF1037c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667152.1 1049985 R 393115 CDS YP_667153.1 110670596 4198961 complement(1050492..1050689) 1 NC_008245.1 a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA; 30S ribosomal protein S21 1050689 rpsU 4198961 rpsU Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S21 YP_667153.1 1050492 R 393115 CDS YP_667154.1 110670597 4199524 1050945..1052348 1 NC_008245.1 Similar to Q83FD1 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase from Coxiella burnetii (477 aa). FASTA: opt: 654 Z-score: 780.3 E(): 1.4e-35 Smith-Waterman score: 654; 29.436 identity in 479 aa overlap.; D-alanyl-D-alanine carboxypeptidase 1052348 dacB1 4199524 dacB1 Francisella tularensis subsp. tularensis FSC198 D-alanyl-D-alanine carboxypeptidase YP_667154.1 1050945 D 393115 CDS YP_667155.1 110670598 4200679 1052349..1052960 1 NC_008245.1 Similar to Q8EBG1 Conserved hypothetical protein from Shewanella oneidensis (205 aa). FASTA: opt: 610 Z-score: 776.5 E(): 2.3e-35 Smith-Waterman score: 610; 45.810 identity in 179 aa overlap. ORF ftt1040; lipoprotein 1052960 4200679 FTF1040 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667155.1 1052349 D 393115 CDS YP_667156.1 110670599 4200680 1052976..1053392 1 NC_008245.1 Similar to Q8ESG1 Hypothetical conserved protein from Oceanobacillus iheyensis (134 aa). FASTA: opt: 215 Z-score: 303.3 E(): 5.3e-09 Smith-Waterman score: 215; 32.773 identity in 119 aa overlap. ORF ftt1041; hypothetical protein 1053392 4200680 FTF1041 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667156.1 1052976 D 393115 CDS YP_667157.1 110670600 4200681 1053385..1054188 1 NC_008245.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 1054188 4200681 FTF1042 Francisella tularensis subsp. tularensis FSC198 C32 tRNA thiolase YP_667157.1 1053385 D 393115 CDS YP_667158.1 110670601 4200682 1054202..1055014 1 NC_008245.1 Similar to O32766 Macrophage infectivity potentiator from Legionella hackeliae (249 aa). FASTA: opt: 235 Z-score: 273.3 E(): 2.5e-07 Smith-Waterman score: 343; 27.599 identity in 279 aa overlap. ORF ftt1043; FKBP-type peptidyl-prolyl cis-trans isomerase family protein 1055014 4200682 FTF1043 Francisella tularensis subsp. tularensis FSC198 FKBP-type peptidyl-prolyl cis-trans isomerase family protein YP_667158.1 1054202 D 393115 CDS YP_667159.1 110670602 4199299 complement(1055032..1056009) 1 NC_008245.1 Similar to Q8EB79 Octaprenyl-diphosphate synthase from Shewanella oneidensis (323 aa). FASTA: opt: 1117 Z-score: 1360.2 E(): 7.1e-68 Smith-Waterman score: 1117; 53.704 identity in 324 aa overlap.; octaprenyl-diphosphate synthase 1056009 ispB 4199299 ispB Francisella tularensis subsp. tularensis FSC198 octaprenyl-diphosphate synthase YP_667159.1 1055032 R 393115 CDS YP_667160.1 110670603 4200683 complement(1056099..1056530) 1 NC_008245.1 ORF ftt1045c; hypothetical protein 1056530 4200683 FTF1045c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667160.1 1056099 R 393115 CDS YP_667161.1 110670604 4200684 complement(1056611..1057234) 1 NC_008245.1 ORF ftt1046c; hypothetical protein 1057234 4200684 FTF1046c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667161.1 1056611 R 393115 CDS YP_667162.1 110670605 4200685 complement(1057906..1058268) 1 NC_008245.1 ORF ftt1047c; hypothetical protein 1058268 4200685 FTF1047c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667162.1 1057906 R 393115 CDS YP_667163.1 110670606 4200686 complement(1058320..1058886) 1 NC_008245.1 ORF ftt1048c; hypothetical protein 1058886 4200686 FTF1048c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667163.1 1058320 R 393115 CDS YP_667164.1 110670607 4199522 complement(1059932..1061344) 1 NC_008245.1 Similar to CYSN_VIBPA (Q87SX9) Sulfate adenylyltransferase subunit 1 from Vibrio parahaemolyticus (476 aa). FASTA: opt: 1823 Z-score: 2170.8 E(): 5e-113 Smith-Waterman score: 1823; 59.488 identity in 469 aa overlap.; sulfate adenylyltransferase 1061344 cysN 4199522 cysN Francisella tularensis subsp. tularensis FSC198 sulfate adenylyltransferase YP_667164.1 1059932 R 393115 CDS YP_667165.1 110670608 4199801 complement(1061783..1061947) 1 NC_008245.1 ORF ftt1051c; hypothetical protein 1061947 4199801 FTF1051c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667165.1 1061783 R 393115 CDS YP_667166.1 110670609 4199802 complement(1062407..1062613) 1 NC_008245.1 ORF ftt1052c; hypothetical protein 1062613 4199802 FTF1052c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667166.1 1062407 R 393115 CDS YP_667167.1 110670610 4199218 complement(1064341..1064829) 1 NC_008245.1 Similar to RIMI_ECOLI (P09453) Ribosomal-protein-alanine acetyltransferase (148 aa). FASTA: opt: 237 Z-score: 305.5 E(): 4e-09 Smith-Waterman score: 237; 36.585 identity in 123 aa overlap.; ribosomal-protein-alanine acetyltransferase 1064829 rimI 4199218 rimI Francisella tularensis subsp. tularensis FSC198 ribosomal-protein-alanine acetyltransferase YP_667167.1 1064341 R 393115 CDS YP_667168.1 110670611 4199804 complement(1064807..1065232) 1 NC_008245.1 ORF ftt1055c; hypothetical protein 1065232 4199804 FTF1055c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667168.1 1064807 R 393115 CDS YP_667169.1 110670612 4199221 complement(1065232..1066047) 1 NC_008245.1 Similar to Q8ZEG0 Ribosomal large subunit pseudouridine synthase B from Yersinia pestis (318 aa). FASTA: opt: 938 Z-score: 1164.9 E(): 5.4e-57 Smith-Waterman score: 938; 56.705identity in 261 aa overlap.; ribosomal large subunit pseudouridine synthase B 1066047 rluB 4199221 rluB Francisella tularensis subsp. tularensis FSC198 ribosomal large subunit pseudouridine synthase B YP_667169.1 1065232 R 393115 CDS YP_667170.1 110670613 4199805 complement(1066040..1066945) 1 NC_008245.1 Similar to Q9KRM4 Fimbrial biogenesis and twitching motility protein, putative from Vibrio cholerae (237 aa). FASTA: opt: 249 Z-score: 293.4 E(): 1.9e-08 Smith-Waterman score: 249; 25.346 identity in 217 aa overlap. Tetratrico peptide repeat (TPR) containing protein. Shows a weak similarity to the lipoprotein pilF (Pseudomonas) that has an N-terminal type II (lipoprotein) signal sequence,followed by six tandem degenerated 34-amino acid repeats,which are involved in protein-protein interactions. ORF ftt1057c; Type IV pili lipoprotein 1066945 4199805 FTF1057c Francisella tularensis subsp. tularensis FSC198 Type IV pili lipoprotein YP_667170.1 1066040 R 393115 CDS YP_667171.1 110670614 4199806 complement(1066935..1068047) 1 NC_008245.1 Similar to Y2A6_PSEAE (Q51385) Hypothetical protein PA3806 from Pseudomonas aeruginosa (379 aa). FASTA: opt: 1458 Z-score: 1752.5 E(): 1e-89 Smith-Waterman score: 1458; 60.434 identity in 369 aa overlap. ORF ftt1058c; radical SAM superfamily protein 1068047 4199806 FTF1058c Francisella tularensis subsp. tularensis FSC198 radical SAM superfamily protein YP_667171.1 1066935 R 393115 CDS YP_667172.1 110670615 4199351 complement(1068078..1069475) 1 NC_008245.1 Similar to DNAB_PASMU (Q9CNL6) Replicative DNA helicase from Pasteurella multocida (467 aa). FASTA: opt: 1484 Z-score: 1719.2 E(): 7.2e-88 Smith-Waterman score: 1484; 51.016 identity in 443 aa overlap.; replicative DNA helicase 1069475 dnaB 4199351 dnaB Francisella tularensis subsp. tularensis FSC198 replicative DNA helicase YP_667172.1 1068078 R 393115 CDS YP_667173.1 110670616 4200779 complement(1069563..1070018) 1 NC_008245.1 in Escherichia coli this protein is wrapped around the base of the L1 stalk; 50S ribosomal protein L9 1070018 rplI 4200779 rplI Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L9 YP_667173.1 1069563 R 393115 CDS YP_667174.1 110670617 4198956 complement(1070037..1070255) 1 NC_008245.1 binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit; 30S ribosomal protein S18 1070255 rpsR 4198956 rpsR Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S18 YP_667174.1 1070037 R 393115 CDS YP_667175.1 110670618 4200338 complement(1070271..1070606) 1 NC_008245.1 binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21; 30S ribosomal protein S6 1070606 rpsF 4200338 rpsF Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S6 YP_667175.1 1070271 R 393115 CDS YP_667176.1 110670619 4200375 1071284..1072210 1 NC_008245.1 catalyzes the conversion of the propionic acid groups of rings I and III to vinyl groups during heme synthesis; coproporphyrinogen III oxidase 1072210 hemF 4200375 hemF Francisella tularensis subsp. tularensis FSC198 coproporphyrinogen III oxidase YP_667176.1 1071284 D 393115 CDS YP_667177.1 110670620 4199807 1072235..1072825 1 NC_008245.1 Shows weak similarity to histidine acid phosphatase family. ORF ftt1064; hypothetical protein 1072825 4199807 FTF1064 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667177.1 1072235 D 393115 CDS YP_667178.1 110670621 4199638 complement(join(1073364..1073822,1073822..1074148)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1074148 isftu1 4199638 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667178.1 1073364 R 393115 CDS YP_667179.1 110670622 4199808 complement(1074317..1074691) 1 NC_008245.1 ORF ftt1066c; hypothetical protein 1074691 4199808 FTF1066c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667179.1 1074317 R 393115 CDS YP_667180.1 110670623 4199809 complement(1078007..1078585) 1 NC_008245.1 ORF ftt1068c; hypothetical protein 1078585 4199809 FTF1068c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667180.1 1078007 R 393115 CDS YP_667181.1 110670624 4199810 complement(1078674..1079579) 1 NC_008245.1 ORF ftt1069c; hypothetical protein 1079579 4199810 FTF1069c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667181.1 1078674 R 393115 CDS YP_667182.1 110670625 4199812 complement(1082848..1083354) 1 NC_008245.1 Similar to Q82XK5 Putative antirestriction protein from Nitrosomonas europaea (171 aa). FASTA: opt: 426 Z-score: 519.1 E(): 5e-21 Smith-Waterman score: 426; 42.771 identity in 166 aa overlap. ORF ftt1071c; hypothetical protein 1083354 4199812 FTF1071c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667182.1 1082848 R 393115 CDS YP_667183.1 110670626 4199813 1083998..1084627 1 NC_008245.1 ORF ftt1072; hypothetical protein 1084627 4199813 FTF1072 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667183.1 1083998 D 393115 CDS YP_667184.1 110670627 4199814 complement(1084783..1085154) 1 NC_008245.1 ORF ftt1073c; hypothetical protein 1085154 4199814 FTF1073c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667184.1 1084783 R 393115 CDS YP_667185.1 110670628 4199639 join(1085388..1085714,1085714..1086172) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1086172 isftu1 4199639 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667185.1 1085388 D 393115 CDS YP_667186.1 110670629 4199815 1087056..1087283 1 NC_008245.1 Similar to Y4MF_RHISN (P55565) Hypothetical transcriptional regulator y4mF from Rhizobium sp. (strain NGR234) (76 aa). FASTA: opt: 205 Z-score: 292.4 E(): 2.1e-08 Smith-Waterman score: 205; 47.059identity in 68 aa overlap. ORF ftt1075; transcriptional regulator 1087283 4199815 FTF1075 Francisella tularensis subsp. tularensis FSC198 transcriptional regulator YP_667186.1 1087056 D 393115 CDS YP_667187.1 110670630 4200194 1087268..1088542 1 NC_008245.1 Similar to Q82V03 Possible HipA protein from Nitrosomonas europaea (429 aa). FASTA: opt: 1021 Z-score: 1249.8 E(): 1e-61 Smith-Waterman score: 1021; 41.162 identity in 413 aa overlap.; transcriptional regulator 1088542 hipA 4200194 hipA Francisella tularensis subsp. tularensis FSC198 transcriptional regulator YP_667187.1 1087268 D 393115 CDS YP_667188.1 110670631 4199816 complement(1088666..1089052) 1 NC_008245.1 Putative pseudogene. Bases 3-128 similar to the same homolog as for next upstream ORF, FRPC_NEIMC (P55127) Iron-regulated protein frpC from Neisseria meningitidis (serogroup C) (1829 aa). FASTA: opt: 118 Z-score: 143.0 bits: 35.9 E(): 4.5 Smith-Waterman score: 120; 26.351 identity in 148 aa overlap (3-128:582-728). ORF ftt1077c; hypothetical protein 1089052 4199816 FTF1077c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667188.1 1088666 R 393115 CDS YP_667189.1 110670632 4199817 complement(1089033..1089845) 1 NC_008245.1 Similar to FRPC_NEIMC (P55127) Iron-regulated protein frpC from Neisseria meningitidis (serogroup C) (1829 aa). FASTA: opt: 567 Z-score: 644.8 E(): 5.1e-28 Smith-Waterman score: 567; 53.416identity in 161 aa overlap (103-260:414-574). Putative pseudogene. Bases 103-260 similar to the same homolog as for the next downstream ORF. ORF ftt1078c; hypothetical protein 1089845 4199817 FTF1078c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667189.1 1089033 R 393115 CDS YP_667190.1 110670633 4199818 complement(1089860..1090120) 1 NC_008245.1 ORF ftt1079c; hypothetical protein 1090120 4199818 FTF1079c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667190.1 1089860 R 393115 CDS YP_667191.1 110670634 4199819 complement(1090137..1091015) 1 NC_008245.1 ORF ftt1080c; hypothetical protein 1091015 4199819 FTF1080c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667191.1 1090137 R 393115 CDS YP_667192.1 110670635 4199573 complement(1091015..1091818) 1 NC_008245.1 ORF ftt1081c; hypothetical protein 1091818 4199573 FTF1081c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667192.1 1091015 R 393115 CDS YP_667193.1 110670636 4199574 1092044..1092466 1 NC_008245.1 ORF ftt1082; hypothetical protein 1092466 4199574 FTF1082 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667193.1 1092044 D 393115 CDS YP_667194.1 110670637 4199575 complement(1092482..1092739) 1 NC_008245.1 ORF ftt1083c; hypothetical protein 1092739 4199575 FTF1083c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667194.1 1092482 R 393115 CDS YP_667195.1 110670638 4200598 complement(1092744..1093604) 1 NC_008245.1 Required for efficient pilin antigenic variation; recombination associated protein 1093604 rdgC 4200598 rdgC Francisella tularensis subsp. tularensis FSC198 recombination associated protein YP_667195.1 1092744 R 393115 CDS YP_667196.1 110670639 4199576 1093786..1094784 1 NC_008245.1 Similar to YM90_SHEON (Q8EES8) Hypothetical UPF0176 protein SO229 from Shewanella oneidensis (333 aa). FASTA: initn: 1561 init1: 1472 opt: 1479 Z-score: 1729.8 E(): 1.9e-88 Smith-Waterman score: 1479; 63.636identity in 330 aa overlap. ORF ftt1085; hypothetical protein 1094784 4199576 FTF1085 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667196.1 1093786 D 393115 CDS YP_667197.1 110670640 4199577 complement(1094915..1095745) 1 NC_008245.1 Similar to Q8EFU7 Conserved hypothetical protein from Shewanella oneidensis (279 aa). FASTA: 351 opt: 789 Z-score: 963.7 E(): 8.8e-46 Smith-Waterman score: 789; 45.290 identity in 276 aa overlap. ORF ftt1086c; hypothetical protein 1095745 4199577 FTF1086c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667197.1 1094915 R 393115 CDS YP_667198.1 110670641 4200610 complement(1095801..1097816) 1 NC_008245.1 Similar to REP_HAEIN (P44804) ATP-dependent DNA helicase rep from Haemophilus influenzae (670 aa). FASTA: opt: 2055 Z-score: 2350.5 E(): 5e-123 Smith-Waterman score: 2055; 47.806 identity in 661 aa overlap.; ATP-dependent DNA helicase 1097816 rep 4200610 rep Francisella tularensis subsp. tularensis FSC198 ATP-dependent DNA helicase YP_667198.1 1095801 R 393115 CDS YP_667199.1 110670642 4199578 complement(1097810..1098556) 1 NC_008245.1 ORF ftt1088c; hypothetical protein 1098556 4199578 FTF1088c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667199.1 1097810 R 393115 CDS YP_667200.1 110670643 4199579 1099164..1099685 1 NC_008245.1 Similar to Q8EW31 Hypothetical protein from Mycoplasma penetrans (194 aa). FASTA: opt: 235 Z-score: 294.7 E(): 1.6e-08 Smith-Waterman score: 235; 31.515 identity in 165 aa overlap. ORF ftt1089; isochorismatase hydrolase family protein 1099685 4199579 FTF1089 Francisella tularensis subsp. tularensis FSC198 isochorismatase hydrolase family protein YP_667200.1 1099164 D 393115 CDS YP_667201.1 110670644 4199580 1099682..1100359 1 NC_008245.1 Similar to Q8A546 Putative membrane transporter involved in nicotinamide mononucleotide transport from Bacteroides thetaiotaomicron (188 aa). FASTA: opt: 285 Z-score: 336.8 E(): 7.2e-11 Smith-Waterman score: 302; 29.592 identity in 196 aa overlap. ORF ftt1090; hypothetical protein 1100359 4199580 FTF1090 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667201.1 1099682 D 393115 CDS YP_667202.1 110670645 4199581 1100372..1100860 1 NC_008245.1 Similar to Q9AD62 Putative hydrolase (193 aa). FASTA: opt: 251 Z-score: 311.7 E(): 1.8e-09 Smith-Waterman score: 251; 32.075identity in 159 aa overlap. ORF ftt1091; isochorismatase hydrolase family protein 1100860 4199581 FTF1091 Francisella tularensis subsp. tularensis FSC198 isochorismatase hydrolase family protein YP_667202.1 1100372 D 393115 CDS YP_667203.1 110670646 4199582 complement(1101017..1101475) 1 NC_008245.1 ORF ftt1092c; hypothetical protein 1101475 4199582 FTF1092c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667203.1 1101017 R 393115 CDS YP_667204.1 110670647 4199095 complement(1101498..1102463) 1 NC_008245.1 Similar to Q8EBH2 Transaldolase from Shewanella oneidensis (318 aa). FASTA: opt: 1030 Z-score: 1249.3 E(): 1.1e-61 Smith-Waterman score: 1030; 50.938 identity in 320 aa overlap.; transaldolase B 1102463 talA 4199095 talA Francisella tularensis subsp. tularensis FSC198 transaldolase B YP_667204.1 1101498 R 393115 CDS YP_667205.1 110670648 4199583 complement(1102597..1104012) 1 NC_008245.1 Similar to Q83DX0 Cytosol aminopeptidase family protein from Coxiella burnetii (458 aa). FASTA: opt: 1273 Z-score: 1536.1 E(): 1.1e-77 Smith-Waterman score: 1273; 41.830 identity in 459 aa overlap. ORF ftt1094c; cytosol aminopeptidase 1104012 4199583 FTF1094c Francisella tularensis subsp. tularensis FSC198 cytosol aminopeptidase YP_667205.1 1102597 R 393115 CDS YP_667206.1 110670649 4199584 complement(1103979..1105361) 1 NC_008245.1 Similar to Q88DA4 Outer membrane efflux protein from Pseudomonas putida (strain KT2440) (477 aa). FASTA: opt: 405 Z-score: 484.1 E(): 4.5e-19 Smith-Waterman score: 405; 22.907 identity in 454 aa overlap. Not a membrane protein. ORF ftt1095c; hypothetical protein 1105361 4199584 FTF1095c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667206.1 1103979 R 393115 CDS YP_667207.1 110670650 4199759 complement(1105369..1107966) 1 NC_008245.1 Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; alanyl-tRNA synthetase 1107966 alaS 4199759 alaS Francisella tularensis subsp. tularensis FSC198 alanyl-tRNA synthetase YP_667207.1 1105369 R 393115 CDS YP_667208.1 110670651 4199585 1108122..1108568 1 NC_008245.1 ORF ftt1097; hypothetical protein 1108568 4199585 FTF1097 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667208.1 1108122 D 393115 CDS YP_667209.1 110670652 4199587 complement(1110657..1110992) 1 NC_008245.1 ORF ftt1099c; hypothetical protein 1110992 4199587 FTF1099c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667209.1 1110657 R 393115 CDS YP_667210.1 110670653 4199588 1111095..1111430 1 NC_008245.1 Similar to Q83DY7 Iojap-related protein from Coxiella burnetii (116 aa). FASTA: opt: 326 Z-score: 425.2 E(): 8.6e-16 Smith-Waterman score: 326; 41.509 identity in 106 aa overlap. ORF ftt1100; hypothetical protein 1111430 4199588 FTF1100 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667210.1 1111095 D 393115 CDS YP_667211.1 110670654 4199590 1112915..1114012 1 NC_008245.1 Similar to Q8Z9P6 Putative exported protein from Salmonella typhi (281 aa). FASTA: opt: 392 Z-score: 435.6 E(): 2.3e-16 Smith-Waterman score: 392; 33.491 identity in 212 aa overlap. ORF ftt1103; lipoprotein 1114012 4199590 FTF1103 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667211.1 1112915 D 393115 CDS YP_667212.1 110670655 4200100 complement(1115496..1116350) 1 NC_008245.1 this fusion consists of methionine sulfoxide B reductase at the N-terminus and A at the C-terminus; A and B are stereospecific enzymes that recognize the damaged produces of oxidative stress, S and R epimers of methionine sulfoxide, respectively; a fusion protein of these enzymes with thioredoxin provides protection against oxidative stress in Neisseria gonorrhoeae; bifunctional methionine sulfoxide reductase B/A protein 1116350 msrA1 4200100 msrA1 Francisella tularensis subsp. tularensis FSC198 bifunctional methionine sulfoxide reductase B/A protein YP_667212.1 1115496 R 393115 CDS YP_667213.1 110670656 4198973 1116479..1116955 1 NC_008245.1 Similar to Q8TID9 Benzodiazepine receptor TspO from Methanosarcina acetivorans (160 aa). FASTA: opt: 417 Z-score: 464.9 E(): 5.3e-18 Smith-Waterman score: 417; 40.994 identity in 161 aa overlap.; tryptophan-rich sensory protein 1116955 tspO 4198973 tspO Francisella tularensis subsp. tularensis FSC198 tryptophan-rich sensory protein YP_667213.1 1116479 D 393115 CDS YP_667214.1 110670657 4199591 1119041..1119793 1 NC_008245.1 Similar to Q891T6 23S rRNA methyltransferase (EC 2.1.1.-) from Clostridium tetani (260 aa). FASTA: opt: 442 Z-score: 545.7 E(): 1.7e-22 Smith-Waterman score: 442; 34.091 identity in 264 aa overlap. ORF ftt1108; rRNA methyltransferase 1119793 4199591 FTF1108 Francisella tularensis subsp. tularensis FSC198 rRNA methyltransferase YP_667214.1 1119041 D 393115 CDS YP_667215.1 110670658 4200211 1119887..1120855 1 NC_008245.1 Similar to Q8U768 Choloylglycine hydrolase family protein from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (355 aa). FASTA: opt: 362 Z-score: 436.7 E(): 2e-16 Smith-Waterman score: 424; 28.956 identity in 297 aa overlap. ORF ftt1109; choloylglycine hydrolase family protein 1120855 4200211 FTF1109 Francisella tularensis subsp. tularensis FSC198 choloylglycine hydrolase family protein YP_667215.1 1119887 D 393115 CDS YP_667216.1 110670659 4200212 1121020..1121373 1 NC_008245.1 ORF ftt1110; hypothetical protein 1121373 4200212 FTF1110 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667216.1 1121020 D 393115 CDS YP_667217.1 110670660 4199292 1121469..1122212 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 1122212 isftu2 4199292 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_667217.1 1121469 D 393115 CDS YP_667218.1 110670661 4200331 complement(1122260..1123138) 1 NC_008245.1 binds with the catalytic core of RNA polymerase to produce the holoenzyme; this sigma factor is responsible for the expression of heat shock promoters; RNA polymerase factor sigma-32 1123138 rpoH 4200331 rpoH Francisella tularensis subsp. tularensis FSC198 RNA polymerase factor sigma-32 YP_667218.1 1122260 R 393115 CDS YP_667219.1 110670662 4200213 complement(1123162..1123692) 1 NC_008245.1 ORF ftt1113c; hypothetical protein 1123692 4200213 FTF1113c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667219.1 1123162 R 393115 CDS YP_667220.1 110670663 4200738 complement(1123724..1124668) 1 NC_008245.1 forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF; preprotein translocase subunit SecF 1124668 secF 4200738 secF Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecF YP_667220.1 1123724 R 393115 CDS YP_667221.1 110670664 4200736 complement(1124687..1126624) 1 NC_008245.1 part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; preprotein translocase subunit SecD 1126624 secD 4200736 secD Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecD YP_667221.1 1124687 R 393115 CDS YP_667222.1 110670665 4200699 complement(1126681..1127037) 1 NC_008245.1 Similar to Q8XFR4 (Q8XFR4) Putative membrane protein (Preprotein translocase IISP family, membrane subunit) from Salmonella typhi (110 aa). FASTA: opt: 347 Z-score: 458.8 E(): 1.2e-17 Smith-Waterman score: 347; 47.664 identity in 107 aa overlap.; preprotein translocase family protein 1127037 yajC 4200699 yajC Francisella tularensis subsp. tularensis FSC198 preprotein translocase family protein YP_667222.1 1126681 R 393115 CDS YP_667223.1 110670666 4200214 complement(1127591..1128163) 1 NC_008245.1 Similar to Q9HZE2 Hypothetical protein PA3066 from Pseudomonas aeruginosa (190 aa). FASTA: opt: 359 Z-score: 477.3 E(): 1.1e-18 Smith-Waterman score: 359; 35.638 identity in 188 aa overlap. ORF ftt1117c; isochorismatase hydrolase family protein 1128163 4200214 FTF1117c Francisella tularensis subsp. tularensis FSC198 isochorismatase hydrolase family protein YP_667223.1 1127591 R 393115 CDS YP_667224.1 110670667 4200215 complement(1128175..1129050) 1 NC_008245.1 Similar to Q9KKY7 Hypothetical protein VCA0963 from Vibrio cholerae (307 aa). FASTA: opt: 340 Z-score: 385.2 E(): 1.5e-13 Smith-Waterman score: 340; 22.603 identity in 292 aa overlap. ORF ftt1118c; hypothetical protein 1129050 4200215 FTF1118c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667224.1 1128175 R 393115 CDS YP_667225.1 110670668 4200216 1129155..1130030 1 NC_008245.1 Similar to Q8D460 Transcriptional regulator from Vibrio vulnificus (296 aa). FASTA: opt: 459 Z-score: 575.9 E(): 3.5e-24 Smith-Waterman score: 459; 29.197 identity in 274 aa overlap. ORF ftt1119; LysR transcriptional regulator family protein 1130030 4200216 FTF1119 Francisella tularensis subsp. tularensis FSC198 LysR transcriptional regulator family protein YP_667225.1 1129155 D 393115 CDS YP_667226.1 110670669 4199138 complement(1130037..1131140) 1 NC_008245.1 Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr); queuine tRNA-ribosyltransferase 1131140 tgt 4199138 tgt Francisella tularensis subsp. tularensis FSC198 queuine tRNA-ribosyltransferase YP_667226.1 1130037 R 393115 CDS YP_667227.1 110670670 4200218 complement(1132114..1132584) 1 NC_008245.1 ORF ftt1122c; lipoprotein 1132584 4200218 FTF1122c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667227.1 1132114 R 393115 CDS YP_667228.1 110670671 4199101 1133166..1133780 1 NC_008245.1 Similar to Y647_YERPE (Q8ZI67) Hypothetical UPF0078 protein YPO0647/Y3532 (216 aa). FASTA: opt: 604 Z-score: 713.6 E(): 7.4e-32 Smith-Waterman score: 604; 48.500 identity in 200 aa overlap.; hypothetical protein 1133780 ygiH 4199101 ygiH Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667228.1 1133166 D 393115 CDS YP_667229.1 110670672 4199840 1133743..1134819 1 NC_008245.1 Similar to AAP15732 ATP-binding component of a transporter from Shigella flexneri (343 aa). FASTA: opt: 1070 Z-score: 1232.1 E(): 9.7e-61 Smith-Waterman score: 1070; 46.221 identity in 344 aa overlap.; D-methionine transport protein, ABC transporter,ATP-binding subunit 1134819 metN 4199840 metN Francisella tularensis subsp. tularensis FSC198 D-methionine transport protein, ABC transporter,ATP-binding subunit YP_667229.1 1133743 D 393115 CDS YP_667230.1 110670673 4199838 1134797..1136269 1 NC_008245.1 Similar to METQ_SALTI (Q8Z992) D-methionine-binding lipoprotein metQ [Precursor] from Salmonella typhi (271 aa). FASTA: opt: 815 Z-score: 926.6 E(): 1e-43 Smith-Waterman score: 815; 51.020identity in 245 aa overlap. The FASTA-hits do not include the first 220 aa. Probably two genes, metI and metQ, in other organisms.; D-methionine binding transport protein, ABC transporter, membrane and periplasmic protein 1136269 metIQ 4199838 metIQ Francisella tularensis subsp. tularensis FSC198 D-methionine binding transport protein, ABC transporter, membrane and periplasmic protein YP_667230.1 1134797 D 393115 CDS YP_667231.1 110670674 4200219 1136340..1137551 1 NC_008245.1 Similar to Q83BG9 Tryptophan/tyrosine permease family protein from Coxiella burnetii (426 aa). FASTA: opt: 560 Z-score: 604.8 E(): 8.5e-26 Smith-Waterman score: 560; 27.750identity in 400 aa overlap. ORF ftt1126; aromatic amino acid HAAP transporter 1137551 4200219 FTF1126 Francisella tularensis subsp. tularensis FSC198 aromatic amino acid HAAP transporter YP_667231.1 1136340 D 393115 CDS YP_667232.1 110670675 4200220 1137617..1138375 1 NC_008245.1 Similar to Q83F83 Rhodanese domain protein from Coxiella burnetii (124 aa). FASTA: opt: 447 Z-score: 559.4 E(): 2.9e-23 Smith-Waterman score: 447; 51.240 identity in 121 aa overlap. ORF ftt1127; rhodanese-like family protein 1138375 4200220 FTF1127 Francisella tularensis subsp. tularensis FSC198 rhodanese-like family protein YP_667232.1 1137617 D 393115 CDS YP_667233.1 110670676 4199302 1138388..1138867 1 NC_008245.1 catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 1138867 ispF 4199302 ispF Francisella tularensis subsp. tularensis FSC198 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase YP_667233.1 1138388 D 393115 CDS YP_667234.1 110670677 4200221 complement(1138872..1140557) 1 NC_008245.1 Similar to CPHA_ANAVA (O86109) Cyanophycin synthetase from Anabaena variabilis (901 aa). FASTA: opt: 2470 Z-score: 2850.7 E(): 6.8e-151 Smith-Waterman score: 2506; 46.787 identity in 887 aa overlap. Probable paralog of cphA since the protein sequences show considerable divergence and gene length differ. ORF ftt1129c; hypothetical protein 1140557 4200221 FTF1129c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667234.1 1138872 R 393115 CDS YP_667235.1 110670678 4200273 complement(1140583..1143405) 1 NC_008245.1 catalyze the formation of cyanophycin which may act to store excess nitrogen; cyanophycin synthetase 1143405 cphA 4200273 cphA Francisella tularensis subsp. tularensis FSC198 cyanophycin synthetase YP_667235.1 1140583 R 393115 CDS YP_667236.1 110670679 4199797 complement(1144261..1145013) 1 NC_008245.1 Similar to Q83DQ4 Glycerophosphoryl diester phosphodiesterase,putative (250 aa). FASTA: opt: 306 Z-score: 366.0 E(): 1.7e-12 Smith-Waterman score: 306; 28.916identity in 249 aa overlap.; glycerophosphoryl diester phosphodiesterase 1145013 glpQ 4199797 glpQ Francisella tularensis subsp. tularensis FSC198 glycerophosphoryl diester phosphodiesterase YP_667236.1 1144261 R 393115 CDS YP_667237.1 110670680 4199729 1145172..1146950 1 NC_008245.1 Similar to O68431 Pilus assembly protein PilB from Pseudomonas syringae (pv. tomato) (575 aa). FASTA: opt: 1381 Z-score: 1521.6 E(): 7.3e-77 Smith-Waterman score: 1592; 44.597 identity in 583 aa overlap. PilB is required for pili assembly. Homologous to pilT but which has the opposite effect. Homologs exists in other systems.; Type IV pili nucleotide binding protein, ABC transporter ATP-binding protein 1146950 pilB 4199729 pilB Francisella tularensis subsp. tularensis FSC198 Type IV pili nucleotide binding protein, ABC transporter ATP-binding protein YP_667237.1 1145172 D 393115 CDS YP_667238.1 110670681 4199730 1146965..1148194 1 NC_008245.1 Similar to Q888U1 Type IV pilus biogenesis protein PilC from Pseudomonas syringae (pv. tomato) (405 aa). FASTA: opt: 1017 Z-score: 1153.2 E(): 2.4e-56 Smith-Waterman score: 1017; 39.055identity in 402 aa overlap. Termed pilF in N. gonorrhoeae.; Type IV pili polytopic inner membrane protein 1148194 pilC 4199730 pilC Francisella tularensis subsp. tularensis FSC198 Type IV pili polytopic inner membrane protein YP_667238.1 1146965 D 393115 CDS YP_667239.1 110670682 4200224 complement(1149438..1149773) 1 NC_008245.1 ORF ftt1136c; hypothetical protein 1149773 4200224 FTF1136c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667239.1 1149438 R 393115 CDS YP_667240.1 110670683 4200225 complement(1149790..1150050) 1 NC_008245.1 ORF ftt1137c; hypothetical protein 1150050 4200225 FTF1137c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667240.1 1149790 R 393115 CDS YP_667241.1 110670684 4200376 1150184..1151185 1 NC_008245.1 protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic; ferrochelatase 1151185 hemH 4200376 hemH Francisella tularensis subsp. tularensis FSC198 ferrochelatase YP_667241.1 1150184 D 393115 CDS YP_667242.1 110670685 4200226 1153402..1153599 1 NC_008245.1 ORF ftt1140; hypothetical protein 1153599 4200226 FTF1140 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667242.1 1153402 D 393115 CDS YP_667243.1 110670686 4199640 complement(join(1153853..1154311,1154311..1154637)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1154637 isftu1 4199640 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667243.1 1153853 R 393115 CDS YP_667244.1 110670687 4200228 1154974..1155219 1 NC_008245.1 ORF ftt1143; hypothetical protein 1155219 4200228 FTF1143 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667244.1 1154974 D 393115 CDS YP_667245.1 110670688 4200321 1156275..1156481 1 NC_008245.1 Similar to Q8XGJ2 Cation transport regulator ChaB from Salmonella typhi (76 aa). FASTA: opt: 267 Z-score: 394.9 E(): 4.2e-14 Smith-Waterman score: 267; 54.412 identity in 68 aa overlap.; cation transport regulator 1156481 chaB 4200321 chaB Francisella tularensis subsp. tularensis FSC198 cation transport regulator YP_667245.1 1156275 D 393115 CDS YP_667246.1 110670689 4199347 complement(1157589..1158764) 1 NC_008245.1 Similar to DFP_HAEIN (P44953) DNA/pantothenate metabolism flavoprotein homolog from Haemophilus influenzae (400 aa). FASTA: opt: 1012 Z-score: 1206.6 E(): 2.6e-59 Smith-Waterman score: 1012; 43.577identity in 397 aa overlap.; 4'-phosphopantothenoylcysteine decarboxylase 1158764 dfp 4199347 dfp Francisella tularensis subsp. tularensis FSC198 4'-phosphopantothenoylcysteine decarboxylase YP_667246.1 1157589 R 393115 CDS YP_667247.1 110670690 4199030 complement(1158777..1159961) 1 NC_008245.1 Similar to YJHB_ECOLI (P39352) Hypothetical metabolite transport protein yjhB from Escherichia coli (405 aa). FASTA: opt: 383 Z-score: 393.4 E(): 5.1e-14 Smith-Waterman score: 383; 26.425 identity in 386 aa overlap.; major facilitator transporter 1159961 yjhB 4199030 yjhB Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667247.1 1158777 R 393115 CDS YP_667248.1 110670691 4199460 complement(1160037..1161545) 1 NC_008245.1 Similar to Q8XT33 Probable amino-acid permease transmembrane from Ralstonia solanacearum (Pseudomonas solanacearum) (543 aa). FASTA: opt: 960 Z-score: 1077.6 E(): 3.9e-52 Smith-Waterman score: 969; 35.730 identity in 445 aa overlap. ORF ftt1149c; amino acid transporter family protein 1161545 4199460 FTF1149c Francisella tularensis subsp. tularensis FSC198 amino acid transporter family protein YP_667248.1 1160037 R 393115 CDS YP_667249.1 110670692 4199126 complement(1161705..1165778) 1 NC_008245.1 proline utilization protein A; multifunctional protein that functions in proline catabolism in the first two enzymatic steps resulting in the conversion of proline to glutamate; in Escherichia coli this protein self regulates transcription via a DNA-binding domain at the N-terminus but the protein from Pseudomonas does not have this domain; bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase 1165778 putA 4199126 putA Francisella tularensis subsp. tularensis FSC198 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase YP_667249.1 1161705 R 393115 CDS YP_667250.1 110670693 4199461 complement(1165786..1166073) 1 NC_008245.1 Similar to Q87LM9 Conserved hypothetical protein from Vibrio parahaemolyticus (86 aa). FASTA: opt: 176 Z-score: 242.1 E(): 1.4e-05 Smith-Waterman score: 176; 32.857 identity in 70 aa overlap. ORF ftt1151c; hypothetical protein 1166073 4199461 FTF1151c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667250.1 1165786 R 393115 CDS YP_667251.1 110670694 4199462 1166287..1166670 1 NC_008245.1 ORF ftt1152; hypothetical protein 1166670 4199462 FTF1152 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667251.1 1166287 D 393115 CDS YP_667252.1 110670695 4199463 complement(1166737..1166961) 1 NC_008245.1 ORF ftt1153c; hypothetical protein 1166961 4199463 FTF1153c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667252.1 1166737 R 393115 CDS YP_667253.1 110670696 4199432 complement(1167019..1168098) 1 NC_008245.1 catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis; 3-dehydroquinate synthase 1168098 aroB 4199432 aroB Francisella tularensis subsp. tularensis FSC198 3-dehydroquinate synthase YP_667253.1 1167019 R 393115 CDS YP_667254.1 110670697 4199438 complement(1168095..1168625) 1 NC_008245.1 type I enzyme similar to type II but differentially regulated; major shikimate kinase in fully repressed cells; catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis; shikimate kinase I 1168625 aroK 4199438 aroK Francisella tularensis subsp. tularensis FSC198 shikimate kinase I YP_667254.1 1168095 R 393115 CDS YP_667255.1 110670698 4199732 complement(1168751..1170535) 1 NC_008245.1 Similar to Q8EK21 Type IV pilus biogenesis protein PilQ from Shewanella oneidensis (684 aa). FASTA: opt: 1014 Z-score: 1145.2 E(): 6.8e-56 Smith-Waterman score: 1014; 31.658 identity in 597 aa overlap. The nearest gene neighbours show similarity to pilN, pilO and pilP. Also required for type II protein secretion.; Type IV pilin multimeric outer membrane protein 1170535 pilQ 4199732 pilQ Francisella tularensis subsp. tularensis FSC198 Type IV pilin multimeric outer membrane protein YP_667255.1 1168751 R 393115 CDS YP_667256.1 110670699 4199464 complement(1170548..1171138) 1 NC_008245.1 Similar to Q8DCM3 Tfp pilus assembly protein PilP from Vibrio vulnificus (170 aa). FASTA: opt: 176 Z-score: 222.1 E(): 0.00018 Smith-Waterman score: 176; 29.921 identity in 127 aa overlap. Weak similarity to the lipoprotein pilP, which appears to be required for stabilisation of pilQ multimer formation. The nearest gene neighbours show similarity to pilN, pilO and pilQ, which indicates that this protein could be pilP ORF ftt1157c; Type IV pili lipoprotein 1171138 4199464 FTF1157c Francisella tularensis subsp. tularensis FSC198 Type IV pili lipoprotein YP_667256.1 1170548 R 393115 CDS YP_667257.1 110670700 4199465 complement(1171144..1171740) 1 NC_008245.1 Similar to Q8PH76 Fimbrial assembly membrane protein from Xanthomonas axonopodis (pv. citri) (221 aa). FASTA: opt: 144 Z-score: 177.4 E(): 0.055 Smith-Waterman score: 144; 25.946 identity in 185 aa overlap. Weak similarity to type IV pili glycosylation protein (pilO). The nearest gene neighbours show similarity to pilN, pilP and pilQ, which indicates that this protein could be pilO. PilO is required for glycosylation of Pseudomonas aeruginosa pilin. ORF ftt1158c; Type IV pili glycosylation protein 1171740 4199465 FTF1158c Francisella tularensis subsp. tularensis FSC198 Type IV pili glycosylation protein YP_667257.1 1171144 R 393115 CDS YP_667258.1 110670701 4199466 complement(1171734..1172297) 1 NC_008245.1 Similar to Q52540 Membrane protein PilN from Pseudomonas syringae (199 aa). FASTA: opt: 143 Z-score: 182.6 E(): 0.028 Smith-Waterman score: 143; 23.704 identity in 135 aa overlap. The nearest gene neighbours show similarity to pilO, pilP and pilQ, which indicates that this protein could be pilN. PilN is also required for type II protein secretion. ORF ftt1159c; Type IV pili associated protein 1172297 4199466 FTF1159c Francisella tularensis subsp. tularensis FSC198 Type IV pili associated protein YP_667258.1 1171734 R 393115 CDS YP_667259.1 110670702 4199467 complement(1172299..1173303) 1 NC_008245.1 ORF ftt1160c; hypothetical protein 1173303 4199467 FTF1160c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667259.1 1172299 R 393115 CDS YP_667260.1 110670703 4199758 1173430..1174086 1 NC_008245.1 essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP; adenylate kinase 1174086 adk 4199758 adk Francisella tularensis subsp. tularensis FSC198 adenylate kinase YP_667260.1 1173430 D 393115 CDS YP_667261.1 110670704 4199469 complement(1177427..1177843) 1 NC_008245.1 ORF ftt1163c; hypothetical protein 1177843 4199469 FTF1163c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667261.1 1177427 R 393115 CDS YP_667262.1 110670705 4199678 complement(1177858..1178751) 1 NC_008245.1 Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome; GTP-binding protein Era 1178751 era 4199678 era Francisella tularensis subsp. tularensis FSC198 GTP-binding protein Era YP_667262.1 1177858 R 393115 CDS YP_667263.1 110670706 4199690 complement(1178865..1180058) 1 NC_008245.1 Similar to Q83E19 Aspartate aminotransferase from Coxiella burnetii (394 aa). FASTA: opt: 1067 Z-score: 1292.7 E(): 4.1e-64 Smith-Waterman score: 1067; 41.645 identity in 389 aa overlap.; aspartate aminotransferase 1180058 aspC2 4199690 aspC2 Francisella tularensis subsp. tularensis FSC198 aspartate aminotransferase YP_667263.1 1178865 R 393115 CDS YP_667264.1 110670707 4199470 complement(1180068..1180874) 1 NC_008245.1 Similar to Q8D5X6 Predicted glutamine amidotransferase from Vibrio vulnificus (253 aa). FASTA: opt: 654 Z-score: 813.9 E(): 1.9e-37 Smith-Waterman score: 654; 42.466identity in 219 aa overlap. ORF ftt1166c; glutamine amidotransferases class-II family protein 1180874 4199470 FTF1166c Francisella tularensis subsp. tularensis FSC198 glutamine amidotransferases class-II family protein YP_667264.1 1180068 R 393115 CDS YP_667265.1 110670708 4199471 complement(1180878..1181516) 1 NC_008245.1 Similar to Q8EE04 (Q8EE04) Conserved hypothetical protein from Shewanella oneidensis (236 aa). FASTA: opt: 349 Z-score: 425.3 E(): 8.5e-16 Smith-Waterman score: 359; 32.273 identity in 220 aa overlap. ORF ftt1167c; glycoprotease family protein 1181516 4199471 FTF1167c Francisella tularensis subsp. tularensis FSC198 glycoprotease family protein YP_667265.1 1180878 R 393115 CDS YP_667266.1 110670709 4200234 complement(1181520..1182653) 1 NC_008245.1 Similar to Q99VS2 NagA protein (Probable N-acetylglucosamine-6-phosphate deacetylase) from Staphylococcus aureus (393 aa). FASTA: opt: 1006 Z-score: 1213.0 E(): 1.1e-59 Smith-Waterman score: 1006; 43.523 identity in 386 aa overlap.; N-acetylglucosamine-6-phosphate deacetylase 1182653 nagA 4200234 nagA Francisella tularensis subsp. tularensis FSC198 N-acetylglucosamine-6-phosphate deacetylase YP_667266.1 1181520 R 393115 CDS YP_667267.1 110670710 4199472 1182716..1183588 1 NC_008245.1 Similar to Q8EE00 GTP-binding protein (313 aa). FASTA: opt: 793 Z-score: 926.5 E(): 1e-43 Smith-Waterman score: 793; 44.218 identity in 294 aa overlap. ORF ftt1169; GTP-binding protein 1183588 4199472 FTF1169 Francisella tularensis subsp. tularensis FSC198 GTP-binding protein YP_667267.1 1182716 D 393115 CDS YP_667268.1 110670711 4199473 1183652..1185400 1 NC_008245.1 ORF ftt1170; lipoprotein 1185400 4199473 FTF1170 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667268.1 1183652 D 393115 CDS YP_667269.1 110670712 4200202 complement(1185390..1186856) 1 NC_008245.1 Similar to Q8RN13 HsdM from Campylobacter jejuni (494 aa). FASTA: opt: 2001 Z-score: 2374.3 E(): 2.3e-124 Smith-Waterman score: 2001; 59.719 identity in 499 aa overlap.; DNA-methyltransferase, type I restriction-modification enzyme subunit M 1186856 hsdM 4200202 hsdM Francisella tularensis subsp. tularensis FSC198 DNA-methyltransferase, type I restriction-modification enzyme subunit M YP_667269.1 1185390 R 393115 CDS YP_667270.1 110670713 4200278 complement(1186843..1187274) 1 NC_008245.1 Similar to Q9HSS3 Cold shock protein from Halobacterium sp. (strain NRC-1 / ATCC 700922 / JCM 11081) (74 aa). FASTA: opt: 170 Z-score: 221.1 E(): 0.0002 Smith-Waterman score: 170; 40.323identity in 62 aa overlap. CDS is longer than FASTA matches. Homology is to first 70 aa of protein only and no matches found for 2nd half of CDS; cold shock protein (DNA-binding) 1187274 csp 4200278 csp Francisella tularensis subsp. tularensis FSC198 cold shock protein (DNA-binding) YP_667270.1 1186843 R 393115 CDS YP_667271.1 110670714 4199474 complement(1188786..1188995) 1 NC_008245.1 ORF ftt1174c; hypothetical protein 1188995 4199474 FTF1174c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667271.1 1188786 R 393115 CDS YP_667272.1 110670715 4199475 complement(1189195..1189833) 1 NC_008245.1 ORF ftt1175c; hypothetical protein 1189833 4199475 FTF1175c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667272.1 1189195 R 393115 CDS YP_667273.1 110670716 4199476 complement(1192509..1193567) 1 NC_008245.1 ORF ftt1177c; hypothetical protein 1193567 4199476 FTF1177c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667273.1 1192509 R 393115 CDS YP_667274.1 110670717 4199477 complement(1193624..1193872) 1 NC_008245.1 ORF ftt1178c; hypothetical protein 1193872 4199477 FTF1178c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667274.1 1193624 R 393115 CDS YP_667275.1 110670718 4200308 1194292..1196109 1 NC_008245.1 Similar to Q9KNJ4 GTP-binding protein TypA from Vibrio cholerae (609 aa). FASTA: opt: 2972 Z-score: 3319.9 E(): 5e-177 Smith-Waterman score: 2972; 73.045identity in 601 aa overlap.; GTP binding translational elongation factor Tu and G family protein 1196109 bipA 4200308 bipA Francisella tularensis subsp. tularensis FSC198 GTP binding translational elongation factor Tu and G family protein YP_667275.1 1194292 D 393115 CDS YP_667276.1 110670719 4199283 complement(1197052..1198857) 1 NC_008245.1 Similar to Q8EFL9 Gamma-glutamyltranspeptidase from Shewanella oneidensis (579 aa). FASTA: opt: 2002 Z-score: 2308.7 E(): 1.1e-120 Smith-Waterman score: 2002; 54.545 identity in 572 aa overlap.; gamma-glutamyltranspeptidase 1198857 ggt 4199283 ggt Francisella tularensis subsp. tularensis FSC198 gamma-glutamyltranspeptidase YP_667276.1 1197052 R 393115 CDS YP_667277.1 110670720 4200250 complement(1199909..1201114) 1 NC_008245.1 Similar to YFCK_ECO57 (Q8XCQ7) Hypothetical protein yfcK from Escherichia coli (668 aa). FASTA: opt: 495 Z-score: 572.0 E(): 5.7e-24 Smith-Waterman score: 495; 28.087 identity in 413 aa overlap. ORF ftt1183c; lipoprotein 1201114 4200250 FTF1183c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667277.1 1199909 R 393115 CDS YP_667278.1 110670721 4200251 complement(1201156..1201461) 1 NC_008245.1 Similar to Y369_VIBVU (Q8DF55) Hypothetical protein VV10369 from Vibrio vulnificus (117 aa). FASTA: opt: 238 Z-score: 320.2 E(): 6.1e-10 Smith-Waterman score: 238; 41.304 identity in 92 aa overlap. ORF ftt1184c; hypothetical protein 1201461 4200251 FTF1184c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667278.1 1201156 R 393115 CDS YP_667279.1 110670722 4200252 complement(1201461..1201892) 1 NC_008245.1 Similar to Q8ZH13 Hypothetical protein YPO1102 from Yersinia pestis (144 aa). FASTA: opt: 463 Z-score: 623.4 E(): 7.9e-27 Smith-Waterman score: 463; 43.662 39dentity in 142 aa overlap. ORF ftt1185c; hypothetical protein 1201892 4200252 FTF1185c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667279.1 1201461 R 393115 CDS YP_667280.1 110670723 4199076 1201967..1202440 1 NC_008245.1 binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation; SsrA-binding protein 1202440 smpB 4199076 smpB Francisella tularensis subsp. tularensis FSC198 SsrA-binding protein YP_667280.1 1201967 D 393115 CDS YP_667281.1 110670724 4200253 1202441..1203196 1 NC_008245.1 TtcA; YdaO; catalyzes the thiolation of cytosine 32 in specific tRNAs; C32 tRNA thiolase 1203196 4200253 FTF1187 Francisella tularensis subsp. tularensis FSC198 C32 tRNA thiolase YP_667281.1 1202441 D 393115 CDS YP_667282.1 110670725 4200254 1203468..1204103 1 NC_008245.1 ORF ftt1188; hypothetical protein 1204103 4200254 FTF1188 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667282.1 1203468 D 393115 CDS YP_667283.1 110670726 4200695 complement(1205899..1207284) 1 NC_008245.1 bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides; exodeoxyribonuclease VII large subunit 1207284 xseA 4200695 xseA Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease VII large subunit YP_667283.1 1205899 R 393115 CDS YP_667284.1 110670727 4200256 1207437..1208597 1 NC_008245.1 Similar to Q8RFU4 N-acyl-L-amino acid amidohydrolase from Fusobacterium nucleatum (subsp. nucleatum) (393 aa). FASTA: opt: 945 Z-score: 1170.3 E(): 2.7e-57 Smith-Waterman score: 945; 38.259 identity in 379 aa overlap. ORF ftt1191; aminoacylase 1208597 4200256 FTF1191 Francisella tularensis subsp. tularensis FSC198 aminoacylase YP_667284.1 1207437 D 393115 CDS YP_667285.1 110670728 4200258 complement(1209880..1210398) 1 NC_008245.1 Similar to Q8ZBY8 Hypothetical protein YPO3242 (Putative amidotransferase) from Yersinia pestis (257 aa). FASTA: opt: 251 Z-score: 317.2 E(): 8.9e-10 Smith-Waterman score: 251; 26.609 identity in 233 aa overlap. ORF ftt1193c; hypothetical protein 1210398 4200258 FTF1193c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667285.1 1209880 R 393115 CDS YP_667286.1 110670729 4200259 complement(1210415..1211341) 1 NC_008245.1 Similar to Q884M8 Conserved hypothetical protein from Pseudomonas syringae (pv. tomato) (319 aa). FASTA: opt: 460 Z-score: 588.5 E(): 6.9e-25 Smith-Waterman score: 460; 30.435identity in 322 aa overlap. Putative pseudogene (upstream sequence matches homologs). ORF ftt1194c; lipoprotein 1211341 4200259 FTF1194c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667286.1 1210415 R 393115 CDS YP_667287.1 110670730 4200261 complement(1213016..1214299) 1 NC_008245.1 Similar to Q9ZC87 Proline/betaine transporter (PROP7) from Rickettsia prowazekii (423 aa). FASTA: opt: 746 Z-score: 810.0 E(): 3.2e-37 Smith-Waterman score: 746; 31.450identity in 407 aa overlap. ORF ftt1196c; major facilitator transporter 1214299 4200261 FTF1196c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667287.1 1213016 R 393115 CDS YP_667288.1 110670731 4200111 complement(1214384..1215181) 1 NC_008245.1 Similar to MURI_NEIMC (Q9RMM3) Glutamate racemase (EC 5.1.1.3) from Neisseria meningitidis (serogroup C) (270 aa). FASTA: opt: 826 Z-score: 958.8 E(): 1.6e-45 Smith-Waterman score: 826; 48.473identity in 262 aa overlap.; glutamate racemase 1215181 murI 4200111 murI Francisella tularensis subsp. tularensis FSC198 glutamate racemase YP_667288.1 1214384 R 393115 CDS YP_667289.1 110670732 4200262 complement(1215181..1216011) 1 NC_008245.1 ORF ftt1198c; hypothetical protein 1216011 4200262 FTF1198c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667289.1 1215181 R 393115 CDS YP_667290.1 110670733 4200134 complement(1216015..1218021) 1 NC_008245.1 The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion; excinuclease ABC subunit B 1218021 uvrB 4200134 uvrB Francisella tularensis subsp. tularensis FSC198 excinuclease ABC subunit B YP_667290.1 1216015 R 393115 CDS YP_667291.1 110670734 4198968 complement(1218023..1219471) 1 NC_008245.1 Similar to Q83BX4 Exodeoxyribonuclease I,putative from Coxiella burnetii (458 aa). FASTA: opt: 677 Z-score: 797.9 E(): 1.5e-36 Smith-Waterman score: 1190; 39.875 identity in 479 aa overlap.; exodeoxyribonuclease I 1219471 sbcB 4198968 sbcB Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease I YP_667291.1 1218023 R 393115 CDS YP_667292.1 110670735 4200263 complement(1219605..1220327) 1 NC_008245.1 Similar to Q9KKP5 Oxidoreductase,short-chain dehydrogenase/reductase family from Vibrio cholerae (239 aa). FASTA: opt: 1097 Z-score: 1344.5 E(): 5.4e-67 Smith-Waterman score: 1097; 72.458identity in 236 aa overlap. ORF ftt1201c; oxidoreductase, short-chain dehydrogenase family protein 1220327 4200263 FTF1201c Francisella tularensis subsp. tularensis FSC198 oxidoreductase, short-chain dehydrogenase family protein YP_667292.1 1219605 R 393115 CDS YP_667293.1 110670736 4200264 1220443..1221342 1 NC_008245.1 Similar to Q9KKP4 Transcriptional regulator, LysR family from Vibrio cholerae (301 aa). FASTA: opt: 661 Z-score: 794.3 E(): 2.4e-36 Smith-Waterman score: 661; 36.120identity in 299 aa overlap. ORF ftt1202; LysR family transcriptional regulator 1221342 4200264 FTF1202 Francisella tularensis subsp. tularensis FSC198 LysR family transcriptional regulator YP_667293.1 1220443 D 393115 CDS YP_667294.1 110670737 4200265 complement(1221353..1222258) 1 NC_008245.1 ORF ftt1203c; hypothetical protein 1222258 4200265 FTF1203c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667294.1 1221353 R 393115 CDS YP_667295.1 110670738 4200266 complement(1222245..1223327) 1 NC_008245.1 ORF ftt1204c; hypothetical protein 1223327 4200266 FTF1204c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667295.1 1222245 R 393115 CDS YP_667296.1 110670739 4199284 1223470..1225353 1 NC_008245.1 GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs; tRNA uridine 5-carboxymethylaminomethyl modification protein GidA 1225353 gidA 4199284 gidA Francisella tularensis subsp. tularensis FSC198 tRNA uridine 5-carboxymethylaminomethyl modification protein GidA YP_667296.1 1223470 D 393115 CDS YP_667297.1 110670740 4200477 1225365..1225757 1 NC_008245.1 ORF ftt1206; hypothetical protein 1225757 4200477 FTF1206 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667297.1 1225365 D 393115 CDS YP_667298.1 110670741 4200478 1225771..1227141 1 NC_008245.1 ORF ftt1207; hypothetical protein 1227141 4200478 FTF1207 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667298.1 1225771 D 393115 CDS YP_667299.1 110670742 4200772 1227143..1227817 1 NC_008245.1 Catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway; ribose-5-phosphate isomerase A 1227817 rpiA 4200772 rpiA Francisella tularensis subsp. tularensis FSC198 ribose-5-phosphate isomerase A YP_667299.1 1227143 D 393115 CDS YP_667300.1 110670743 4199641 complement(join(1229891..1230349,1230349..1230675)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1230675 isftu1 4199641 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667300.1 1229891 R 393115 CDS YP_667301.1 110670744 4200480 complement(1230737..1231129) 1 NC_008245.1 CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein ORF ftt1211c; hypothetical protein 1231129 4200480 FTF1211c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667301.1 1230737 R 393115 CDS YP_667302.1 110670745 4199791 complement(1231129..1231512) 1 NC_008245.1 Similar to LGUL_VIBPA (P46235) Probable lactoylglutathione lyase from Vibrio parahaemolyticus (138 aa). FASTA: opt: 549 Z-score: 759.1 E(): 2.2e-34 Smith-Waterman score: 549; 64.516 identity in 124 aa overlap.; lactoylglutathione lyase 1231512 gloA 4199791 gloA Francisella tularensis subsp. tularensis FSC198 lactoylglutathione lyase YP_667302.1 1231129 R 393115 CDS YP_667303.1 110670746 4200481 1231628..1232341 1 NC_008245.1 Similar to YPDP_BACSU (P54163) Hypothetical protein ypdP from Bacillus subtilis (229 aa). FASTA: opt: 472 Z-score: 563.3 E(): 1.7e-23 Smith-Waterman score: 472; 40.435 identity in 230 aa overlap. ORF ftt1213; YhhQ family protein 1232341 4200481 FTF1213 Francisella tularensis subsp. tularensis FSC198 YhhQ family protein YP_667303.1 1231628 D 393115 CDS YP_667304.1 110670747 4200482 complement(1232595..1233386) 1 NC_008245.1 Similar to Q8XN14 Hypothetical protein CPE0524 from Clostridium perfringens (264 aa). FASTA: opt: 486 Z-score: 584.5 E(): 1.1e-24 Smith-Waterman score: 486; 31.939 identity in 263 aa overlap. ORF ftt1214c; haloacid dehalogenase-like hydrolase family protein 1233386 4200482 FTF1214c Francisella tularensis subsp. tularensis FSC198 haloacid dehalogenase-like hydrolase family protein YP_667304.1 1232595 R 393115 CDS YP_667305.1 110670748 4200594 complement(1234021..1235043) 1 NC_008245.1 Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step; S-adenosylmethionine--tRNA ribosyltransferase-isomerase 1235043 queA 4200594 queA Francisella tularensis subsp. tularensis FSC198 S-adenosylmethionine--tRNA ribosyltransferase-isomerase YP_667305.1 1234021 R 393115 CDS YP_667306.1 110670749 4200483 complement(1235063..1235524) 1 NC_008245.1 ORF ftt1216c; hypothetical protein 1235524 4200483 FTF1216c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667306.1 1235063 R 393115 CDS YP_667307.1 110670750 4200140 complement(1235546..1236781) 1 NC_008245.1 Similar to Q83F72 VisC protein from Coxiella burnetii (411 aa). FASTA: opt: 645 Z-score: 778.5 E(): 1.8e-35 Smith-Waterman score: 858; 37.192 identity in 406 aa overlap.; monooxygenase family protein 1236781 visC 4200140 visC Francisella tularensis subsp. tularensis FSC198 monooxygenase family protein YP_667307.1 1235546 R 393115 CDS YP_667308.1 110670751 4198980 complement(1236771..1237982) 1 NC_008245.1 Similar to Q87UH4 2-octaprenyl-6-methoxyphenyl hydroxylase from Pseudomonas syringae (pv. tomato) (395 aa). FASTA: opt: 654 Z-score: 770.5 E(): 5e-35 Smith-Waterman score: 654; 32.143 identity in 392 aa overlap.; 2-octaprenyl-6-methoxyphenyl hydroxylase 1237982 ubiH 4198980 ubiH Francisella tularensis subsp. tularensis FSC198 2-octaprenyl-6-methoxyphenyl hydroxylase YP_667308.1 1236771 R 393115 CDS YP_667309.1 110670752 4200484 complement(1237991..1238527) 1 NC_008245.1 Similar to YGA1_PSEPK (Q88CI0) Hypothetical UPF0149 protein PP5201 from Pseudomonas putida (strain KT2440) (184 aa). FASTA: opt: 269 Z-score: 349.3 E(): 1.4e-11 Smith-Waterman score: 269; 34.637identity in 179 aa overlap. ORF ftt1219c; hypothetical protein 1238527 4200484 FTF1219c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667309.1 1237991 R 393115 CDS YP_667310.1 110670753 4200485 1238928..1239476 1 NC_008245.1 Similar to Q8ZM70 Putative ligase from Salmonella typhimurium (182 aa). FASTA: opt: 317 Z-score: 411.1 E(): 5.2e-15 Smith-Waterman score: 317; 32.727 identity in 165 aa overlap. ORF ftt1220; 5-formyltetrahydrofolate cyclo-ligase 1239476 4200485 FTF1220 Francisella tularensis subsp. tularensis FSC198 5-formyltetrahydrofolate cyclo-ligase YP_667310.1 1238928 D 393115 CDS YP_667311.1 110670754 4200486 1239479..1239745 1 NC_008245.1 ORF ftt1221; hypothetical protein 1239745 4200486 FTF1221 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667311.1 1239479 D 393115 CDS YP_667312.1 110670755 4199533 1239752..1240378 1 NC_008245.1 Similar to Q89YU2 Conserved hypothetical protein,putative membrane protein from Bacteroides thetaiotaomicron (213 aa). FASTA: opt: 602 Z-score: 702.4 E(): 3.1e-31 Smith-Waterman score: 602; 42.857identity in 210 aa overlap.; DedA family protein 1240378 dedA2 4199533 dedA2 Francisella tularensis subsp. tularensis FSC198 DedA family protein YP_667312.1 1239752 D 393115 CDS YP_667313.1 110670756 4199532 1240434..1241081 1 NC_008245.1 Similar to Q8R8Y8 Hypothetical protein DedA2 from Thermoanaerobacter tengcongensis (209 aa). FAST: opt: 674 Z-score: 828.7 E(): 2.9e-38 Smith-Waterman score: 674; 45.894 identity in 207 aa overlap.; DedA family protein 1241081 dedA1 4199532 dedA1 Francisella tularensis subsp. tularensis FSC198 DedA family protein YP_667313.1 1240434 D 393115 CDS YP_667314.1 110670757 4200606 complement(1241157..1241855) 1 NC_008245.1 Similar to RECO_VIBVU (Q8DC74) DNA repair protein recO from Vibrio vulnificus (243 aa). FASTA: opt: 244 Z-score: 288.3 E(): 3.6e-08 Smith-Waterman score: 244; 29.130 identity in 230 aa overlap.; DNA repair protein recO 1241855 recO 4200606 recO Francisella tularensis subsp. tularensis FSC198 DNA repair protein recO YP_667314.1 1241157 R 393115 CDS YP_667315.1 110670758 4200487 complement(1241839..1242168) 1 NC_008245.1 ORF ftt1225c; hypothetical protein 1242168 4200487 FTF1225c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667315.1 1241839 R 393115 CDS YP_667316.1 110670759 4199613 complement(1242161..1243336) 1 NC_008245.1 Similar to ISCS_VIBCH (Q9KTY2) Cysteine desulfurase (EC 4.4.1.-) from Vibrio cholerae (404 aa). FASTA: opt: 1104 Z-score: 1336.0 E(): 1.6e-66 Smith-Waterman score: 1104; 45.812 identity in 382 aa overlap.; cysteine desulfarase 1243336 iscS 4199613 iscS Francisella tularensis subsp. tularensis FSC198 cysteine desulfarase YP_667316.1 1242161 R 393115 CDS YP_667317.1 110670760 4199227 1243586..1246243 1 NC_008245.1 Similar to Q9KQG8 Ribonuclease E from Vibrio cholerae (1052 aa). FASTA: opt: 1928 Z-score: 1743.2 E(): 3.3e-89 Smith-Waterman score: 1943; 43.377 identity in 770 aa overlap.; ribonuclease E 1246243 rne 4199227 rne Francisella tularensis subsp. tularensis FSC198 ribonuclease E YP_667317.1 1243586 D 393115 CDS YP_667318.1 110670761 4199515 1246258..1247064 1 NC_008245.1 transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein; prolipoprotein diacylglyceryl transferase 1247064 lgt 4199515 lgt Francisella tularensis subsp. tularensis FSC198 prolipoprotein diacylglyceryl transferase YP_667318.1 1246258 D 393115 CDS YP_667319.1 110670762 4199144 1247049..1247873 1 NC_008245.1 ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived; thymidylate synthase 1247873 thyA 4199144 thyA Francisella tularensis subsp. tularensis FSC198 thymidylate synthase YP_667319.1 1247049 D 393115 CDS YP_667320.1 110670763 4200741 1247876..1249120 1 NC_008245.1 catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate; D-3-phosphoglycerate dehydrogenase 1249120 serA 4200741 serA Francisella tularensis subsp. tularensis FSC198 D-3-phosphoglycerate dehydrogenase YP_667320.1 1247876 D 393115 CDS YP_667321.1 110670764 4199665 1249121..1250317 1 NC_008245.1 Similar to Q83BJ9 MesJ protein from Coxiella burnetii (449 aa). FASTA: opt: 639 Z-score: 733.1 E(): 6.1e-33 Smith-Waterman score: 726; 31.387identity in 411 aa overlap. Thought to be a cell cycle protein; PP-loop family protein 1250317 mesJ 4199665 mesJ Francisella tularensis subsp. tularensis FSC198 PP-loop family protein YP_667321.1 1249121 D 393115 CDS YP_667322.1 110670765 4199642 join(1250462..1250788,1250788..1251246) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1251246 isftu1 4199642 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667322.1 1250462 D 393115 CDS YP_667323.1 110670766 4199111 complement(1251320..1252648) 1 NC_008245.1 Similar to YJDL_ECOLI (P39276) Hypothetical transporter yjdL from Escherichia coli (485 aa).FASTA: opt: 1064 Z-score: 1135.9 E(): 2.2e-55 Smith-Waterman score: 1064; 38.222 identity in 450 aa overlap.This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein; proton-dependent oligopeptide transport (POT) family protein 1252648 yjdL 4199111 yjdL Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_667323.1 1251320 R 393115 CDS YP_667324.1 110670767 4200488 1252899..1254044 1 NC_008245.1 Similar to Q8YIA2 Choloylglycine hydrolase (EC 3.5.1.24) from Brucella melitensis (367 aa). FASTA: opt: 387 Z-score: 460.1 E(): 9.7e-18 Smith-Waterman score: 574; 34.718 identity in 337 aa overlap. ORF ftt1234; choloylglycine hydrolase family protein 1254044 4200488 FTF1234 Francisella tularensis subsp. tularensis FSC198 choloylglycine hydrolase family protein YP_667324.1 1252899 D 393115 CDS YP_667325.1 110670768 4199564 complement(1254045..1255109) 1 NC_008245.1 Similar to Q8FZH8 Lipopolysaccharide core biosynthesis mannosyltransferase LpcC from Brucella suis (354 aa). FASTA: opt: 825 Z-score: 1005.6 E(): 4e-48 Smith-Waterman score: 825; 38.352 identity in 352 aa overlap.; glycosyl transferases group 1 family protein 1255109 lpcC 4199564 lpcC Francisella tularensis subsp. tularensis FSC198 glycosyl transferases group 1 family protein YP_667325.1 1254045 R 393115 CDS YP_667326.1 110670769 4200489 1255279..1256367 1 NC_008245.1 ORF ftt1236; hypothetical protein 1256367 4200489 FTF1236 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667326.1 1255279 D 393115 CDS YP_667327.1 110670770 4200490 1256375..1257265 1 NC_008245.1 Similar to Q8A3T1 Putative stress protein from Bacteroides thetaiotaomicron (309 aa). FASTA: opt: 308 Z-score: 375.2 E(): 5.2e-13 Smith-Waterman score: 348; 26.599 identity in 297 aa overlap. ORF ftt1237; glycosyl transferase family protein 1257265 4200490 FTF1237 Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_667327.1 1256375 D 393115 CDS YP_667328.1 110670771 4200491 complement(1257291..1258622) 1 NC_008245.1 ORF ftt1238c; hypothetical protein 1258622 4200491 FTF1238c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667328.1 1257291 R 393115 CDS YP_667329.1 110670772 4200492 1258830..1260101 1 NC_008245.1 ORF ftt1239; hypothetical protein 1260101 4200492 FTF1239 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667329.1 1258830 D 393115 CDS YP_667330.1 110670773 4200493 complement(1260116..1260664) 1 NC_008245.1 Similar to YJGA_HAEIN (P45076) Hypothetical protein HI1151 from Haemophilus influenzae (178 aa). FASTA: opt: 359 Z-score: 390.6 E(): 7.3e-14 Smith-Waterman score: 359; 35.928 identity in 167 aa overlap. ORF ftt1240c; hypothetical protein 1260664 4200493 FTF1240c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667330.1 1260116 R 393115 CDS YP_667331.1 110670774 4200288 1260768..1262021 1 NC_008245.1 catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate; serine hydroxymethyltransferase 1262021 glyA 4200288 glyA Francisella tularensis subsp. tularensis FSC198 serine hydroxymethyltransferase YP_667331.1 1260768 D 393115 CDS YP_667332.1 110670775 4200494 1262303..1263565 1 NC_008245.1 ORF ftt1242; hypothetical protein 1263565 4200494 FTF1242 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667332.1 1262303 D 393115 CDS YP_667333.1 110670776 4199854 complement(1263447..1265300) 1 NC_008245.1 Similar to EAA32028 Hypothetical protein from Neurospora crassa (567 aa). FASTA: opt: 643 Z-score: 759.7 E(): 2e-34 Smith-Waterman score: 793; 29.879 identity in 579 aa overlap.; FAD binding family protein 1265300 mreA 4199854 mreA Francisella tularensis subsp. tularensis FSC198 FAD binding family protein YP_667333.1 1263447 R 393115 CDS YP_667334.1 110670777 4199100 complement(1265377..1266201) 1 NC_008245.1 Similar to Q8XYX9 Probable transmembrane protein from Ralstonia solanacearum (Pseudomonas solanacearum) (289 aa). FASTA: opt: 563 Z-score: 689.8 E(): 1.6e-30 Smith-Waterman score: 563; 32.558 identity in 258 aa overlap.; lipoprotein 1266201 yfiO 4199100 yfiO Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667334.1 1265377 R 393115 CDS YP_667335.1 110670778 4199223 1266290..1267288 1 NC_008245.1 Similar to Q87S65 Ribosomal large subunit pseudouridine from Vibrio parahaemolyticus (325 aa). FASTA: opt: 917 Z-score: 1118.2 E(): 2.2e-54 Smith-Waterman score: 917; 47.468 identity in 316 aa overlap.; ribosomal large subunit pseudouridine synthase D 1267288 rluD 4199223 rluD Francisella tularensis subsp. tularensis FSC198 ribosomal large subunit pseudouridine synthase D YP_667335.1 1266290 D 393115 CDS YP_667336.1 110670779 4200495 1267230..1268078 1 NC_008245.1 Similar to Q8EEM6 Bax protein, putative from Shewanella oneidensis (255 aa). FASTA: opt: 369 Z-score: 448.5 E(): 4.3e-17 Smith-Waterman score: 369; 31.081 identity in 222 aa overlap. ORF ftt1246; hypothetical protein 1268078 4200495 FTF1246 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667336.1 1267230 D 393115 CDS YP_667337.1 110670780 4200496 1268153..1269253 1 NC_008245.1 Similar to Q9A5X9 ABC transporter,permease protein,putative from Caulobacter crescentus.(371 aa). FASTA: opt: 755 Z-score: 860.3 E(): 5e-40 Smith-Waterman score: 755; 36.095 identity in 338 aa overlap. ORF ftt1247; ABC transporter membrane protein 1269253 4200496 FTF1247 Francisella tularensis subsp. tularensis FSC198 ABC transporter membrane protein YP_667337.1 1268153 D 393115 CDS YP_667338.1 110670781 4200497 1269268..1270029 1 NC_008245.1 Similar to Q83DK2 ABC transporter,ATP-binding protein from Coxiella burnetii (268 aa). FASTA: opt: 674 Z-score: 783.9 E(): 9e-36 Smith-Waterman score: 674; 46.188 identity in 223 aa overlap. ORF ftt1248; ABC transporter ATP-binding protein 1270029 4200497 FTF1248 Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_667338.1 1269268 D 393115 CDS YP_667339.1 110670782 4200498 1270039..1270764 1 NC_008245.1 Similar to Q9HZ27 Hypothetical protein PA3213 from Pseudomonas aeruginosa (312 aa). FASTA: opt: 282 Z-score: 315.9 E(): 1.1e-09 Smith-Waterman score: 282; 23.661 identity in 224 aa overlap. The archetype (Rv0169) for mce related family proteins, was isolated as being necessary for colonization of, and survival within, the macrophage MEDLINE:93377076. ORF ftt1249; cell entry (mce) related family protein 1270764 4200498 FTF1249 Francisella tularensis subsp. tularensis FSC198 cell entry (mce) related family protein YP_667339.1 1270039 D 393115 CDS YP_667340.1 110670783 4200499 1270769..1271467 1 NC_008245.1 ORF ftt1250; hypothetical protein 1271467 4200499 FTF1250 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667340.1 1270769 D 393115 CDS YP_667341.1 110670784 4200500 complement(1271457..1272143) 1 NC_008245.1 Similar to Q8EDF8 Conserved hypothetical protein from Shewanella oneidensis (234 aa). FASTA: opt: 692 Z-score: 805.5 E(): 5.6e-37 Smith-Waterman score: 692; 44.828 identity in 232 aa overlap. ORF ftt1251c; hypothetical protein 1272143 4200500 FTF1251c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667341.1 1271457 R 393115 CDS YP_667342.1 110670785 4200501 1272214..1272942 1 NC_008245.1 in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase; 16S ribosomal RNA methyltransferase RsmE 1272942 4200501 FTF1252 Francisella tularensis subsp. tularensis FSC198 16S ribosomal RNA methyltransferase RsmE YP_667342.1 1272214 D 393115 CDS YP_667343.1 110670786 4200502 1273172..1274659 1 NC_008245.1 Similar to Q9KTB5 Proton/peptide symporter family protein from Vibrio cholerae (514 aa). FASTA: opt: 1234 Z-score: 1376.6 E(): 8.7e-69 Smith-Waterman score: 1245; 42.050identity in 478 aa overlap. ORF ftt1253; proton-dependent oligopeptide transport (POT) family protein 1274659 4200502 FTF1253 Francisella tularensis subsp. tularensis FSC198 proton-dependent oligopeptide transport (POT) family protein YP_667343.1 1273172 D 393115 CDS YP_667344.1 110670787 4199402 1274767..1276458 1 NC_008245.1 Similar to Q89UU0 Long-chain-fatty-acid-CoA ligase from Bradyrhizobium japonicum (561 aa). FASTA: opt: 1308 Z-score: 1621.3 E(): 2.1e-82 Smith-Waterman score: 1308; 38.324 identity in 561 aa overlap.; Acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) 1276458 fadD1 4199402 fadD1 Francisella tularensis subsp. tularensis FSC198 Acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) YP_667344.1 1274767 D 393115 CDS YP_667345.1 110670788 4200503 complement(1276446..1277294) 1 NC_008245.1 Similar to Q8PAT1 Transcriptional regulator from Xanthomonas campestris (pv. campestris) (287 aa). FASTA: opt: 689 Z-score: 866.5 E(): 2.3e-40 Smith-Waterman score: 689; 37.354identity in 257 aa overlap. ORF ftt1255c; transcriptional regulator araC family protein 1277294 4200503 FTF1255c Francisella tularensis subsp. tularensis FSC198 transcriptional regulator araC family protein YP_667345.1 1276446 R 393115 CDS YP_667346.1 110670789 4199674 1277438..1278967 1 NC_008245.1 Similar to Q83DE1 Drug resistance transporter,EmrB/QacA family from Coxiella burnetii (526 aa). FASTA: opt: 1037 Z-score: 1145.4 E(): 6.6e-56 Smith-Waterman score: 1037; 31.928 identity in 498 aa overlap.; major facilitator transporter 1278967 emrB 4199674 emrB Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667346.1 1277438 D 393115 CDS YP_667347.1 110670790 4199672 1278989..1280011 1 NC_008245.1 Similar to Q83DE0 Multidrug resistance protein from Coxiella burnetii (331 aa). FASTA: opt: 742 Z-score: 834.3 E(): 1.4e-38 Smith-Waterman score: 742; 37.695 identity in 321 aa overlap.; HlyD family secretion protein 1280011 emrA1 4199672 emrA1 Francisella tularensis subsp. tularensis FSC198 HlyD family secretion protein YP_667347.1 1278989 D 393115 CDS YP_667348.1 110670791 4200504 1280021..1281493 1 NC_008245.1 Similar to Q93NK3 Putative outer membrane protein from Pseudomonas syringae (pv. syringae) (475 aa). FASTA: opt: 594 Z-score: 697.2 E(): 6e-31 Smith-Waterman score: 594; 28.970 identity in 466 aa overlap. ORF ftt1258; outer membrane efflux protein 1281493 4200504 FTF1258 Francisella tularensis subsp. tularensis FSC198 outer membrane efflux protein YP_667348.1 1280021 D 393115 CDS YP_667349.1 110670792 4200232 1281622..1282371 1 NC_008245.1 Similar to NADE_MYCPN (P75216) Probable NH(3)-dependent NAD(+) synthetase Mycoplasma pneumoniae (248 aa). FASTA: opt: 601 Z-score: 760.1 E(): 1.9e-34 Smith-Waterman score: 601; 40.928identity in 237 aa overlap.; NH(3)-dependent NAD(+) synthetase 1282371 nadE 4200232 nadE Francisella tularensis subsp. tularensis FSC198 NH(3)-dependent NAD(+) synthetase YP_667349.1 1281622 D 393115 CDS YP_667350.1 110670793 4200505 1282475..1282897 1 NC_008245.1 ORF ftt1260; hypothetical protein 1282897 4200505 FTF1260 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667350.1 1282475 D 393115 CDS YP_667351.1 110670794 4199643 join(1283806..1284132,1284132..1284590) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1284590 isftu1 4199643 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667351.1 1283806 D 393115 CDS YP_667352.1 110670795 4200507 complement(1284882..1285877) 1 NC_008245.1 Duplicate of FTF0354 and FTF0378. ORF ftt1263c; hypothetical protein 1285877 4200507 FTF1263c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667352.1 1284882 R 393115 CDS YP_667353.1 110670796 4199644 complement(join(1286806..1287264,1287264..1287590)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1287590 isftu1 4199644 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667353.1 1286806 R 393115 CDS YP_667354.1 110670797 4199105 complement(1287614..1288327) 1 NC_008245.1 Similar to YC10_PSEAE (Q9I4C8) Hypothetical protein PA1210 from Pseudomonas aeruginosa (232 aa). FASTA: opt: 602 Z-score: 765.5 E(): 9.5e-35 Smith-Waterman score: 602; 41.667 identity in 228 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein.; pirin family protein 1288327 yhhW 4199105 yhhW Francisella tularensis subsp. tularensis FSC198 pirin family protein YP_667354.1 1287614 R 393115 CDS YP_667355.1 110670798 4200508 1288427..1289323 1 NC_008245.1 Similar to Q8E7Z8 Transcriptional regulator, LysR family from Shewanella oneidensis (289 aa). FASTA: opt: 561 Z-score: 683.5 E(): 3.5e-30 Smith-Waterman score: 561; 32.423identity in 293 aa overlap. ORF ftt1267; LysR transcriptional regulator family protein 1289323 4200508 FTF1267 Francisella tularensis subsp. tularensis FSC198 LysR transcriptional regulator family protein YP_667355.1 1288427 D 393115 CDS YP_667356.1 110670799 4199354 complement(1289337..1290461) 1 NC_008245.1 chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion; chaperone protein DnaJ 1290461 dnaJ1 4199354 dnaJ1 Francisella tularensis subsp. tularensis FSC198 chaperone protein DnaJ YP_667356.1 1289337 R 393115 CDS YP_667357.1 110670800 4199356 complement(1290550..1292478) 1 NC_008245.1 heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria; molecular chaperone DnaK 1292478 dnaK 4199356 dnaK Francisella tularensis subsp. tularensis FSC198 molecular chaperone DnaK YP_667357.1 1290550 R 393115 CDS YP_667358.1 110670801 4200300 complement(1292579..1293166) 1 NC_008245.1 with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor; heat shock protein GrpE 1293166 grpE 4200300 grpE Francisella tularensis subsp. tularensis FSC198 heat shock protein GrpE YP_667358.1 1292579 R 393115 CDS YP_667359.1 110670802 4199849 1293255..1294421 1 NC_008245.1 Similar to Q81ZZ6 Putative membrane-bound lytic murein transglycosylase A transmembrane protein from Nitrosomonas europaea (440 aa). FASTA: opt: 821 Z-score: 989.7 E(): 3.1e-47 Smith-Waterman score: 821; 39.769 identity in 347 aa overlap.; membrane-bound lytic murein transglycosylase A (MLT) family protein 1294421 mltA 4199849 mltA Francisella tularensis subsp. tularensis FSC198 membrane-bound lytic murein transglycosylase A (MLT) family protein YP_667359.1 1293255 D 393115 CDS YP_667360.1 110670803 4200509 1294414..1295598 1 NC_008245.1 Similar to Q8KB91 Protoporphyrinogen oxidase,putative from Chlorobium tepidum (399 aa). FASTA: opt: 594 Z-score: 698.0 E(): 5.5e-31 Smith-Waterman score: 673; 31.552 identity in 393 aa overlap. ORF ftt1272; hypothetical protein 1295598 4200509 FTF1272 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667360.1 1294414 D 393115 CDS YP_667361.2 162139941 4198989 1295737..1296165 1 NC_008245.1 in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit; 50S ribosomal protein L13 1296165 rplM 4198989 rplM Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L13 YP_667361.2 1295737 D 393115 CDS YP_667362.1 110670805 4200341 1296172..1296570 1 NC_008245.1 forms a direct contact with the tRNA during translation; 30S ribosomal protein S9 1296570 rpsI 4200341 rpsI Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S9 YP_667362.1 1296172 D 393115 CDS YP_667363.1 110670806 4199842 1296630..1297247 1 NC_008245.1 Similar to O52734 Macrophage growth locus A from Francisella novicida strain U112 (205 aa). FASTA: opt: 1275 Z-score: 1587.7 E(): 1.5e-80 Smith-Waterman score: 1275; 99.512 identity in 205 aa overlap (1-205:1-205).; macrophage growth locus subunit A 1297247 mglA 4199842 mglA Francisella tularensis subsp. tularensis FSC198 macrophage growth locus subunit A YP_667363.1 1296630 D 393115 CDS YP_667364.1 110670807 4199843 1297249..1297656 1 NC_008245.1 Identical to O52735 Macrophage growth locus B from Francisella novicida strain U112 (135 aa).; ClpXP protease specificity-enhancing factor 1297656 mglB 4199843 mglB Francisella tularensis subsp. tularensis FSC198 ClpXP protease specificity-enhancing factor YP_667364.1 1297249 D 393115 CDS YP_667365.1 110670808 4200510 complement(1297653..1298924) 1 NC_008245.1 Similar to Q88EE1 Sodium-solute symporter, putative from Pseudomonas putida (strain KT2440) (459 aa). FASTA: opt: 279 Z-score: 311.8 E(): 1.8e-09 Smith-Waterman score: 300; 20.863identity in 417 aa overlap. ORF ftt1277c; Sodium-solute symporter family protein 1298924 4200510 FTF1277c Francisella tularensis subsp. tularensis FSC198 Sodium-solute symporter family protein YP_667365.1 1297653 R 393115 CDS YP_667366.1 110670809 4200767 complement(1298917..1299480) 1 NC_008245.1 RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids; ribonuclease HII 1299480 rnhB 4200767 rnhB Francisella tularensis subsp. tularensis FSC198 ribonuclease HII YP_667366.1 1298917 R 393115 CDS YP_667367.1 110670810 4199099 complement(1299482..1300225) 1 NC_008245.1 Similar to Q8ZCR9 Putative SpoU-family rRNA methylase from Yersinia pestis (257 aa). FASTA: opt: 688 Z-score: 847.8 E(): 2.5e-39 Smith-Waterman score: 688; 47.755 identity in 245 aa overlap.; SpoU rRNA methylase family protein 1300225 yfhQ 4199099 yfhQ Francisella tularensis subsp. tularensis FSC198 SpoU rRNA methylase family protein YP_667367.1 1299482 R 393115 CDS YP_667368.1 110670811 4199949 complement(1300218..1300673) 1 NC_008245.1 Similar to Q88PA0 Phosphotransferase system,nitrogen regulatory IIA protein from Pseudomonas putida (strain KT2440) (154 aa). FASTA: opt: 239 Z-score: 318.2 E(): 7.8e-10 Smith-Waterman score: 239; 26.316identity in 152 aa overlap.; PEP-dependent sugar PTS system family protein 1300673 ptsN 4199949 ptsN Francisella tularensis subsp. tularensis FSC198 PEP-dependent sugar PTS system family protein YP_667368.1 1300218 R 393115 CDS YP_667369.1 110670812 4199103 complement(1300710..1301006) 1 NC_008245.1 Similar to RP5M_PSEPK (P15592) Probable sigma(54) modulation protein from Pseudomonas putida (102 aa). FASTA: opt: 336 Z-score: 439.6 E(): 1.4e-16 Smith-Waterman score: 336; 54.839 identity in 93 aa overlap.; sigma-54 modulation protein 1301006 yhbH 4199103 yhbH Francisella tularensis subsp. tularensis FSC198 sigma-54 modulation protein YP_667369.1 1300710 R 393115 CDS YP_667370.1 110670813 4200511 1301125..1301370 1 NC_008245.1 ORF ftt1282; hypothetical protein 1301370 4200511 FTF1282 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667370.1 1301125 D 393115 CDS YP_667371.1 110670814 4199864 1301376..1302728 1 NC_008245.1 in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE; tRNA modification GTPase TrmE 1302728 trmE 4199864 trmE Francisella tularensis subsp. tularensis FSC198 tRNA modification GTPase TrmE YP_667371.1 1301376 D 393115 CDS YP_667372.1 110670815 4200512 complement(1302738..1302932) 1 NC_008245.1 ORF ftt1284c; hypothetical protein 1302932 4200512 FTF1284c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667372.1 1302738 R 393115 CDS YP_667373.1 110670816 4200513 complement(1302964..1303854) 1 NC_008245.1 Similar to Q8D448 Transcriptional regulator from Vibrio vulnificus (303 aa). FASTA: opt: 372 Z-score: 436.1 E(): 2.1e-16 Smith-Waterman score: 372; 28.571 identity in 294 aa overlap. ORF ftt1285c; LysR transcriptional regulator family protein 1303854 4200513 FTF1285c Francisella tularensis subsp. tularensis FSC198 LysR transcriptional regulator family protein YP_667373.1 1302964 R 393115 CDS YP_667374.1 110670817 4200317 1305262..1306185 1 NC_008245.1 Similar to Q9BH24 Cystathionine beta-synthase 5 from Trypanosoma cruzi (384 aa). FASTA: opt: 540 Z-score: 651.0 E(): 2.3e-28 Smith-Waterman score: 542; 31.494 identity in 308 aa overlap.; cystathionine beta-synthase (cystein synthase) 1306185 cbs 4200317 cbs Francisella tularensis subsp. tularensis FSC198 cystathionine beta-synthase (cystein synthase) YP_667374.1 1305262 D 393115 CDS YP_667375.1 110670818 4200515 1306439..1307008 1 NC_008245.1 Similar to Q8Z651 Putative membrane protein from Salmonella typhi (204 aa). FASTA: opt: 462 Z-score: 553.9 E(): 5.9e-23 Smith-Waterman score: 462; 36.667 identity in 180 aa overlap. ORF ftt1288; hypothetical protein 1307008 4200515 FTF1288 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667375.1 1306439 D 393115 CDS YP_667376.1 110670819 4199837 1313866..1315890 1 NC_008245.1 methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content; methionyl-tRNA synthetase 1315890 metG 4199837 metG Francisella tularensis subsp. tularensis FSC198 methionyl-tRNA synthetase YP_667376.1 1313866 D 393115 CDS YP_667377.1 110670820 4200517 1315856..1317130 1 NC_008245.1 Similar to EXUT_BACSU (O34456) Hexuronate transporter from Bacillus subtilis (422 aa). FASTA: opt: 655 Z-score: 716.7 E(): 5e-32 Smith-Waterman score: 659; 28.250 identity in 400 aa overlap. ORF ftt1291; major facilitator transporter 1317130 4200517 FTF1291 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667377.1 1315856 D 393115 CDS YP_667378.1 110670821 4200518 complement(1317127..1317729) 1 NC_008245.1 Similar to Q88YM1 Hypothetical protein from Lactobacillus plantarum (215 aa). FASTA: opt: 420 Z-score: 532.1 E(): 9.5e-22 Smith-Waterman score: 420; 35.025 identity in 197 aa overlap. ORF ftt1292c; hypothetical protein 1317729 4200518 FTF1292c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667378.1 1317127 R 393115 CDS YP_667379.1 110670822 4200519 complement(1317779..1318393) 1 NC_008245.1 Similar to Q9KSS8 Hypothetical protein VC1178 from Vibrio cholerae (206 aa). FASTA: opt: 558 Z-score: 715.6 E(): 5.8e-32 Smith-Waterman score: 558; 42.703 identity in 185 aa overlap. ORF ftt1293c; Sua5_yciO_yrdC family protein 1318393 4200519 FTF1293c Francisella tularensis subsp. tularensis FSC198 Sua5_yciO_yrdC family protein YP_667379.1 1317779 R 393115 CDS YP_667380.1 110670823 4199498 1318454..1318984 1 NC_008245.1 Similar to ISPZ_RALSO (Q8XYL2) Probable intracellular septation protein from Ralstonia solanacearum (Pseudomonas solanacearum) (186 aa). FASTA: opt: 549 Z-score: 659.0 E(): 8.2e-29 Smith-Waterman score: 549; 38.150 identity in 173 aa overlap.; intracellular septation protein A family protein 1318984 ispZ 4199498 ispZ Francisella tularensis subsp. tularensis FSC198 intracellular septation protein A family protein YP_667380.1 1318454 D 393115 CDS YP_667381.1 110670824 4199785 complement(1319323..1320339) 1 NC_008245.1 Similar to GLK_ANASP (P58616) Glucokinase from Anabaena sp. (strain PCC 7120) (342 aa). FASTA: opt: 735 Z-score: 904.6 E(): 1.7e-42 Smith-Waterman score: 735; 36.735 identity in 343 aa overlap.; glucose kinase 1320339 glk1 4199785 glk1 Francisella tularensis subsp. tularensis FSC198 glucose kinase YP_667381.1 1319323 R 393115 CDS YP_667382.1 110670825 4198978 1320433..1321185 1 NC_008245.1 Similar to Q8D1I3 COQ5 methyltransferase from Yersinia pestis (251 aa). FASTA: opt: 1184 Z-score: 1440.7 E(): 2.4e-72 Smith-Waterman score: 1184; 70.800 identity in 250 aa overlap.; menaquinone biosynthesis methyltransferase 1321185 ubiE 4198978 ubiE Francisella tularensis subsp. tularensis FSC198 menaquinone biosynthesis methyltransferase YP_667382.1 1320433 D 393115 CDS YP_667383.1 110670826 4200520 1321169..1321774 1 NC_008245.1 ORF ftt1297; hypothetical protein 1321774 4200520 FTF1297 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667383.1 1321169 D 393115 CDS YP_667384.1 110670827 4198977 1321767..1323425 1 NC_008245.1 Similar to Q83A88 Ubiquinone biosynthesis protein AarF, putative from Coxiella burnetii (541 aa). FASTA: opt: 1682 Z-score: 2006.3 E(): 7.3e-104 Smith-Waterman score: 1682; 48.269 identity in 520 aa overlap. unrelated to the ABC transporter proteins.; 2-polyprenylphenol 6-hydroxylase 1323425 ubiB 4198977 ubiB Francisella tularensis subsp. tularensis FSC198 2-polyprenylphenol 6-hydroxylase YP_667384.1 1321767 D 393115 CDS YP_667385.1 110670828 4200196 1323431..1323769 1 NC_008245.1 Similar to Q82WM8 HIT (Histidine triad) family from Nitrosomonas europaea (116 aa). FASTA: opt: 424 Z-score: 574.5 E(): 4.1e-24 Smith-Waterman score: 424; 53.571 identity in 112 aa overlap.; histidine triad (HIT) family protein 1323769 hitA 4200196 hitA Francisella tularensis subsp. tularensis FSC198 histidine triad (HIT) family protein YP_667385.1 1323431 D 393115 CDS YP_667386.1 110670829 4200521 complement(1323786..1323917) 1 NC_008245.1 ORF ftt1300c; hypothetical protein 1323917 4200521 FTF1300c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667386.1 1323786 R 393115 CDS YP_667387.1 110670830 4200523 1324916..1325746 1 NC_008245.1 ORF ftt1302; hypothetical protein 1325746 4200523 FTF1302 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667387.1 1324916 D 393115 CDS YP_667388.1 110670831 4200524 complement(1325748..1326623) 1 NC_008245.1 ORF ftt1303c; hypothetical protein 1326623 4200524 FTF1303c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667388.1 1325748 R 393115 CDS YP_667389.1 110670832 4200105 complement(1326702..1327550) 1 NC_008245.1 Similar to MURB_THEMA (Q9X239) UDP-N-acetylenolpyruvoylglucosamine reductase from Thermotoga maritima (284 aa). FASTA: opt: 577 Z-score: 699.7 E(): 4.4e-31 Smith-Waterman score: 577; 39.273 identity in 275 aa overlap.; UDP-N-acetylenolpyruvoylglucosamine reductase 1327550 murB 4200105 murB Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylenolpyruvoylglucosamine reductase YP_667389.1 1326702 R 393115 CDS YP_667390.1 110670833 4200104 complement(1327558..1328892) 1 NC_008245.1 Similar to Q814X9 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Bacillus cereus (strain ATCC 14579 / DSM 31) (434 aa). FASTA: opt: 697 Z-score: 859.3 E(): 5.6e-40 Smith-Waterman score: 765; 33.333 identity in 438 aa overlap.; UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1328892 murA 4200104 murA Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylglucosamine 1-carboxyvinyltransferase YP_667390.1 1327558 R 393115 CDS YP_667391.1 110670834 4199675 complement(1328831..1330228) 1 NC_008245.1 EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains; GTP-binding protein EngA 1330228 engA 4199675 engA Francisella tularensis subsp. tularensis FSC198 GTP-binding protein EngA YP_667391.1 1328831 R 393115 CDS YP_667392.1 110670835 4200525 complement(1330444..1330980) 1 NC_008245.1 ORF ftt1307c; hypothetical protein 1330980 4200525 FTF1307c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667392.1 1330444 R 393115 CDS YP_667393.1 110670836 4200526 complement(1331025..1331633) 1 NC_008245.1 ORF ftt1308c; hypothetical protein 1331633 4200526 FTF1308c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667393.1 1331025 R 393115 CDS YP_667394.1 110670837 4200380 complement(1332772..1334688) 1 NC_008245.1 Similar to FTSH_ECO57 (Q8X9L0) Cell division protease ftsH from Escherichia coli O157:H7 (644 aa). FASTA: opt: 2569 Z-score: 2823.5 E(): 2.2e-149 Smith-Waterman score: 2569; 60.125 identity in 642 aa overlap.; ATP-dependent metalloprotease 1334688 hflB 4200380 hflB Francisella tularensis subsp. tularensis FSC198 ATP-dependent metalloprotease YP_667394.1 1332772 R 393115 CDS YP_667395.1 110670838 4199109 1334911..1336317 1 NC_008245.1 Similar to Q8UHQ9 MFS permease from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (475 aa). FASTA: opt: 757 Z-score: 760.1 E(): 1.9e-34 Smith-Waterman score: 766; 32.735 identity in 446 aa overlap.; major facilitator transporter 1336317 yieO 4199109 yieO Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667395.1 1334911 D 393115 CDS YP_667396.1 110670839 4198988 complement(1336323..1339145) 1 NC_008245.1 Similar to UVRA_PSEAE (Q9HWG0) UvrABC system protein A (UvrA protein) from Pseudomonas aeruginosa (945 aa). FASTA: opt: 4062 Z-score: 4339.5 E():0 Smith-Waterman score: 4062; 63.800identity in 942 aa overlap.; DNA excision repair protein subunit A 1339145 uvrA 4198988 uvrA Francisella tularensis subsp. tularensis FSC198 DNA excision repair protein subunit A YP_667396.1 1336323 R 393115 CDS YP_667397.1 110670840 4200297 complement(1339184..1339666) 1 NC_008245.1 necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; transcription elongation factor GreA 1339666 greA 4200297 greA Francisella tularensis subsp. tularensis FSC198 transcription elongation factor GreA YP_667397.1 1339184 R 393115 CDS YP_667398.1 110670841 4200527 complement(1339700..1340284) 1 NC_008245.1 Similar to Q8EBJ4 Type IV pilus biogenesis protein (164 aa). FASTA: opt: 157 Z-score: 204.8 E(): 0.0016 Smith-Waterman score: 157; 27.607 identity in 163 aa overlap. The pili fiber building block protein has a highly distinct primary structure; a short positively charged leader sequence, and a highly conserved and highly hydrophobic amino-terminal domain which forms the core of the pilus fiber; A variety of studies suggest that the invariant glycine (G) at -1 is required for prepilin cleavage and assembly and that the glutamate (E) at + 5 is essential for assembly and efficient N-terminal methylation but not cleavage. The region near the carboxy terminus is often hydrophilic and is stabilized by one or two disulfide bridges that may be involved in recognition of receptors. The genes with these characteristics are usually termed pilA, pilE, pilV, pilW, pilX and FimU. (Mattick John S.. 'Type IV Pili and twitching motility'. Annu. Rev. Microbiol. 2002. 56:289-314.) This protein has all the characteristics described above, except the glutamate (E) at + 5, but shows a weak global homology. ORF ftt1314c; Type IV pili fiber building block protein 1340284 4200527 FTF1314c Francisella tularensis subsp. tularensis FSC198 Type IV pili fiber building block protein YP_667398.1 1339700 R 393115 CDS YP_667399.1 110670842 4199242 complement(1340285..1341907) 1 NC_008245.1 functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family; glucose-6-phosphate isomerase 1341907 pgi 4199242 pgi Francisella tularensis subsp. tularensis FSC198 glucose-6-phosphate isomerase YP_667399.1 1340285 R 393115 CDS YP_667400.1 110670843 4200528 complement(1341894..1342547) 1 NC_008245.1 Similar to Q8R9T9 Thiamine pyrophosphokinase from Thermoanaerobacter tengcongensis (211 aa). FASTA: opt: 221 Z-score: 278.6 E(): 1.3e-07 Smith-Waterman score: 238; 25.943 identity in 212 aa overlap. ORF ftt1316c; thiamine pyrophospokinase 1342547 4200528 FTF1316c Francisella tularensis subsp. tularensis FSC198 thiamine pyrophospokinase YP_667400.1 1341894 R 393115 CDS YP_667401.1 110670844 4200366 complement(1342613..1344073) 1 NC_008245.1 Similar to Q88P22 Inosine-5-monophosphate dehydrogenase from Pseudomonas putida (strain KT2440) (489 aa). FASTA: opt: 2187 Z-score: 2403.4 E(): 5.6e-126 Smith-Waterman score: 2187; 68.033 identity in 488 aa overlap.; inosine-5-monophosphate dehydrogenase 1344073 guaB 4200366 guaB Francisella tularensis subsp. tularensis FSC198 inosine-5-monophosphate dehydrogenase YP_667401.1 1342613 R 393115 CDS YP_667402.1 110670845 4199239 complement(1344174..1345613) 1 NC_008245.1 Similar to Q887M0 Cytosol aminopeptidase from Pseudomonas syringae (pv. tomato) (496 aa). FASTA: opt: 1161 Z-score: 1388.1 E(): 2e-69 Smith-Waterman score: 1165; 42.540 identity in 496 aa overlap.; cytosol aminopeptidase 1345613 pepA 4199239 pepA Francisella tularensis subsp. tularensis FSC198 cytosol aminopeptidase YP_667402.1 1344174 R 393115 CDS YP_667403.1 110670846 4200529 1345779..1346861 1 NC_008245.1 Similar to Q8EH61 Conserved hypothetical protein from Shewanella oneidensis (370 aa). FASTA: opt: 370 Z-score: 433.4 E(): 3e-16 Smith-Waterman score: 370; 21.508 identity in 358 aa overlap. ORF ftt1319; YjgP/YjgQ family permease 1346861 4200529 FTF1319 Francisella tularensis subsp. tularensis FSC198 YjgP/YjgQ family permease YP_667403.1 1345779 D 393115 CDS YP_667404.1 110670847 4200530 1346861..1347922 1 NC_008245.1 Similar to Q8ZK26 Putative permease from Salmonella typhimurium (360 aa). FASTA: opt: 510 Z-score: 585.0 E(): 1.1e-24 Smith-Waterman score: 511; 27.147 identity in 361 aa overlap. ORF ftt1320; YjgP/YjgQ family permease 1347922 4200530 FTF1320 Francisella tularensis subsp. tularensis FSC198 YjgP/YjgQ family permease YP_667404.1 1346861 D 393115 CDS YP_667405.1 110670848 4200531 1347930..1349183 1 NC_008245.1 Similar to Q83AI4 Peptidase, M16 family from Coxiella burnetii (459 aa). FASTA: opt: 868 Z-score: 1053.1 E(): 9.1e-51 Smith-Waterman score: 868; 34.158 identity in 404 aa overlap. ORF ftt1321; peptidase M16 family protein 1349183 4200531 FTF1321 Francisella tularensis subsp. tularensis FSC198 peptidase M16 family protein YP_667405.1 1347930 D 393115 CDS YP_667406.1 110670849 4200532 1349190..1350413 1 NC_008245.1 Similar to Q83AI5 Peptidase, M16 family from Coxiella burnetii (443 aa). FASTA: opt: 605 Z-score: 727.1 E(): 1.3e-32 Smith-Waterman score: 605; 28.607 identity in 402 aa overlap. ORF ftt1322; peptidase M16 family protein 1350413 4200532 FTF1322 Francisella tularensis subsp. tularensis FSC198 peptidase M16 family protein YP_667406.1 1349190 D 393115 CDS YP_667407.1 110670850 4200533 1350414..1350992 1 NC_008245.1 Similar to YHHF_HAEIN (P44869) Putative methylase HI0767 from Haemophilus influenzae (193 aa). FASTA: opt: 455 Z-score: 588.3 E(): 7e-25 Smith-Waterman score: 455; 42.697 identity in 178 aa overlap. ORF ftt1323; methylase 1350992 4200533 FTF1323 Francisella tularensis subsp. tularensis FSC198 methylase YP_667407.1 1350414 D 393115 CDS YP_667408.1 110670851 4200552 1351026..1351676 1 NC_008245.1 Similar to Q9CM99 Hypothetical protein PM0935 from Pasteurella multocida (226 aa). FASTA: opt: 523 Z-score: 599.4 E(): 1.7e-25 Smith-Waterman score: 523; 39.151 identity in 212 aa overlap. ORF ftt1324; hypothetical protein 1351676 4200552 FTF1324 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667408.1 1351026 D 393115 CDS YP_667409.1 110670852 4199845 complement(1351729..1352340) 1 NC_008245.1 Similar to Q88KV1 TRNA-(ms(2)io(6)a)-hydroxylase,putative from Pseudomonas putida (strain KT2440)(205 aa). FASTA: opt: 660 Z-score: 867.5 E(): 2e-40 Smith-Waterman score: 660; 50.777 identity in 193 aa overlap.; tRNA-(ms(2)io(6)a)-hydroxylase 1352340 miaE 4199845 miaE Francisella tularensis subsp. tularensis FSC198 tRNA-(ms(2)io(6)a)-hydroxylase YP_667409.1 1351729 R 393115 CDS YP_667410.1 110670853 4198982 1352456..1353259 1 NC_008245.1 Similar to Q9CKN8 Udp from Pasteurella multocida (252 aa). FASTA: opt: 817 Z-score: 1065.9 E(): 1.8e-51 Smith-Waterman score: 817; 48.760 identity in 242 aa overlap.; uridine phosphorylase 1353259 udp 4198982 udp Francisella tularensis subsp. tularensis FSC198 uridine phosphorylase YP_667410.1 1352456 D 393115 CDS YP_667411.1 110670854 4200319 1353280..1353681 1 NC_008245.1 Similar to Q8RCG7 Cytidine deaminase from Thermoanaerobacter tengcongensis (135 aa). FASTA: opt: 413 Z-score: 565.4 E(): 1.3e-23 Smith-Waterman score: 413; 52.273 identity in 132 aa overlap.; cytidine deaminase 1353681 cdd 4200319 cdd Francisella tularensis subsp. tularensis FSC198 cytidine deaminase YP_667411.1 1353280 D 393115 CDS YP_667412.1 110670855 4200553 complement(1353879..1356908) 1 NC_008245.1 Similar to Q8EDV0 Oxidoreductase,FAD-binding,putative from Shewanella oneidensis (1013 aa). FASTA: opt: 1758 Z-score: 1886.5 E(): 3.1e-97 Smith-Waterman score: 2679; 40.573 identity in 1013 aa overlap. Contains a frameshift after aa 89. ORF ftt1328c; hypothetical protein 1356908 4200553 FTF1328c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667412.1 1353879 R 393115 CDS YP_667413.1 110670856 4200295 1357128..1358666 1 NC_008245.1 catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate; phosphoglyceromutase 1358666 gpmI 4200295 gpmI Francisella tularensis subsp. tularensis FSC198 phosphoglyceromutase YP_667413.1 1357128 D 393115 CDS YP_667414.1 110670857 4200743 1358681..1359961 1 NC_008245.1 catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; seryl-tRNA synthetase 1359961 serS 4200743 serS Francisella tularensis subsp. tularensis FSC198 seryl-tRNA synthetase YP_667414.1 1358681 D 393115 CDS YP_667415.1 110670858 4200276 1360071..1360970 1 NC_008245.1 Similar to Q97IW7 Fructokinase from Clostridium acetobutylicum (288 aa). FASTA: opt: 932 Z-score: 1175.6 E(): 1.4e-57 Smith-Waterman score: 932; 47.603identity in 292 aa overlap.; fructokinase 1360970 cscK 4200276 cscK Francisella tularensis subsp. tularensis FSC198 fructokinase YP_667415.1 1360071 D 393115 CDS YP_667416.1 110670859 4200554 1361048..1362307 1 NC_008245.1 Similar to Q9X4C7 Repeat unit exporter from Escherichia coli (419 aa). FASTA: opt: 324 Z-score: 369.2 E(): 1.1e-12 Smith-Waterman score: 324; 22.604 identity in 407 aa overlap. ORF ftt1332; polysaccharide biosynthesis protein (export protein) 1362307 4200554 FTF1332 Francisella tularensis subsp. tularensis FSC198 polysaccharide biosynthesis protein (export protein) YP_667416.1 1361048 D 393115 CDS YP_667417.1 110670860 4200555 complement(1362311..1362511) 1 NC_008245.1 ORF ftt1333c; hypothetical protein 1362511 4200555 FTF1333c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667417.1 1362311 R 393115 CDS YP_667418.1 110670861 4200556 complement(1362539..1363048) 1 NC_008245.1 ORF ftt1334c; hypothetical protein 1363048 4200556 FTF1334c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667418.1 1362539 R 393115 CDS YP_667419.1 110670862 4200285 1363425..1365206 1 NC_008245.1 in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter membrane/ATP-binding protein 1365206 cydD 4200285 cydD Francisella tularensis subsp. tularensis FSC198 cysteine/glutathione ABC transporter membrane/ATP-binding protein YP_667419.1 1363425 D 393115 CDS YP_667420.1 110670863 4200284 1365203..1366852 1 NC_008245.1 in Escherichia coli the CydCD ABC transporter exports cysteine and glutathione into the periplasm in order to maintain redox balance; important for cytochrome bd and c; cysteine/glutathione ABC transporter membrane/ATP-binding protein 1366852 cydC 4200284 cydC Francisella tularensis subsp. tularensis FSC198 cysteine/glutathione ABC transporter membrane/ATP-binding protein YP_667420.1 1365203 D 393115 CDS YP_667421.1 110670864 4199528 complement(1366854..1368104) 1 NC_008245.1 Similar to DTA2_PSEAE (Q9I4F5) C4-dicarboxylate transport protein from Pseudomonas aeruginosa (436 aa). FASTA: opt: 1534 Z-score: 1703.1 E(): 5.7e-87 Smith-Waterman score: 1534; 55.206identity in 413 aa overlap.; C4-dicarboxylate transport protein 1368104 dctA 4199528 dctA Francisella tularensis subsp. tularensis FSC198 C4-dicarboxylate transport protein YP_667421.1 1366854 R 393115 CDS YP_667422.1 110670865 4200557 complement(1368243..1368623) 1 NC_008245.1 Similar to Q99WA6 SA0455 protein (Translation initiation inhibitor homologue) from Staphylococcus aureus (126 aa). FASTA: 452 opt: 452 Z-score: 597.7 E(): 2.1e-25 Smith-Waterman score: 452; 61.017 identity in 118 aa overlap. ORF ftt1338c; translation initiation inhibitor 1368623 4200557 FTF1338c Francisella tularensis subsp. tularensis FSC198 translation initiation inhibitor YP_667422.1 1368243 R 393115 CDS YP_667423.1 110670866 4200558 complement(1368623..1370167) 1 NC_008245.1 Similar to Q87N13 Sulfate permease family protein from Vibrio parahaemolyticus (519 aa). FASTA: opt: 1333 Z-score: 1472.3 E(): 4.1e-74 Smith-Waterman score: 1602; 47.398 identity in 538 aa overlap. ORF ftt1339c; sulfate permease family protein 1370167 4200558 FTF1339c Francisella tularensis subsp. tularensis FSC198 sulfate permease family protein YP_667423.1 1368623 R 393115 CDS YP_667424.1 110670867 4199294 complement(1370286..1371029) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 1371029 isftu2 4199294 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_667424.1 1370286 R 393115 CDS YP_667425.1 110670868 4200559 1371230..1371886 1 NC_008245.1 Similiar to CAD85637 (Q82TY8) Putative integral membrane transmembrane protein from Nitrosomonas europaea (221 aa). FASTA: 670 Z-score: 817.1 E(): 1.2e-37 50.226identity in 221 aa overlap ORF ftt1341; hypothetical protein 1371886 4200559 FTF1341 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667425.1 1371230 D 393115 CDS YP_667426.1 110670869 4200560 1372000..1373055 1 NC_008245.1 Similar to Q08861 Gamma-aminobutyric acid-grated chloride- ion channel/receptor, zeta subunit, precusor from Lymnaea stagnalis (437 aa). FASTA: opt: 357 Z-score: 407.4 E(): 7.7e-15 Smith-Waterman score: 360; 23.510 identity in 302 aa overlap ORF ftt1342; hypothetical protein 1373055 4200560 FTF1342 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667426.1 1372000 D 393115 CDS YP_667427.1 110670870 4200561 complement(1373350..1374159) 1 NC_008245.1 ORF ftt1343c; hypothetical protein 1374159 4200561 FTF1343c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667427.1 1373350 R 393115 CDS YP_667428.1 110670871 4199230 1379766..1382228 1 NC_008245.1 Similar to AAP58968.1 (Q7X3I9) PdpA from Francisella novicida (820 aa). BLAST Score = 1545 bits (4000), Expect = 0.0 Identities = 777/820 (94), Positives = 787/820 (95) Identical to FTF1699. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1382228 pdpA1 4199230 pdpA1 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667428.1 1379766 D 393115 CDS YP_667429.1 110670872 4199232 1382233..1385514 1 NC_008245.1 Similar to AAP58967.1 (Q7X3J0) PdpB from Francisella novicida (1093 aa). BLAST Score = 1914 bits (4959), Expect = 0.0 Identities = 975/1093 (89),Positives = 988/1093 (90) Identical to FTF1700. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1385514 pdpB1 4199232 pdpB1 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667429.1 1382233 D 393115 CDS YP_667430.1 110670873 4200562 1385504..1385881 1 NC_008245.1 Identical to FTF1701. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1346; hypothetical protein 1385881 4200562 FTF1346 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667430.1 1385504 D 393115 CDS YP_667431.1 110670874 4200563 1385894..1386388 1 NC_008245.1 Similar to AAP58969.1 (Q7XI38) unknown protein from Francisella novicida (164 aa). BLAST Score = 305 bits (780), Expect = 2e-82 Identities = 163/164 (99),Positives = 163/164 (99) Identical to FTF1702. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1347; hypothetical protein 1386388 4200563 FTF1347 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667431.1 1385894 D 393115 CDS YP_667432.1 110670875 4200564 1386393..1388123 1 NC_008245.1 1-213 aa similar to AAP58970.1 (Q7X3I7) unknown Francisella novicida (212 aa). BLAST Score 352 ,Expect = 1e-95 Identities = 177/209 (84), Positives = 189/209 (90). 330-580aa similar to AAP58971.1 (Q7X3I6) unknown protein from Francisella tularensis subsp. novicida (251 aa). BLAST Score = 483 bits (1244), Expect = e-135 Identities = 243/251 (96), Positives = 249/251 (99). Identical to FTF1703. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625. Protein fusion product of two hypothetical proteins in F. novicida. ORF ftt1348; hypothetical protein 1388123 4200564 FTF1348 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667432.1 1386393 D 393115 CDS YP_667433.1 110670876 4200565 1388124..1388645 1 NC_008245.1 Similar to AAP58972.1 (Q7X3I5) unknown protein from Francisella novicida (173 aa). BLAST Score = 336, Expect = 1e-91 Identities = 170/173 (98), Positives = 171/173 (98) Identical to FTF1704. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1349; hypothetical protein 1388645 4200565 FTF1349 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667433.1 1388124 D 393115 CDS YP_667434.1 110670877 4200566 1388658..1390088 1 NC_008245.1 Similar to AAP58973.1 (Q7X3I4) unknown protein from Francisella novicida (476 aa). BLAST Score = 864 bits (2233), Expect = 0.0 Identities = 433/476 (90),Positives = 437/476 (91) Identical to FTF1705. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1350; hypothetical protein 1390088 4200566 FTF1350 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667434.1 1388658 D 393115 CDS YP_667435.1 110670878 4200567 1390101..1390724 1 NC_008245.1 Similar to AAP58974.1 (Q7X3I3) unknown protein from Francisella novicida (207 aa). BLAST Score = 391 bits (1005), Expect = e-108 Identities = 194/207 (93),Positives = 195/207 (94) Identical to FTF1706. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1351; hypothetical protein 1390724 4200567 FTF1351 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667435.1 1390101 D 393115 CDS YP_667436.1 110670879 4200568 1390746..1391897 1 NC_008245.1 Similar to AAP58975.1 (Q7X3I2) unknown protein from Francisella novicida (262 aa), BLAST Score = 443 bits (1140), Expect = e-12 Identities = 229/262 (87),Positives = 235/262 (89) This protein is identical to FTF1707. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1352; hypothetical protein 1391897 4200568 FTF1352 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667436.1 1390746 D 393115 CDS YP_667437.1 110670880 4200569 1391863..1392636 1 NC_008245.1 Similar to AAP58976.1 (Q7X3I1) unknown protein from Francisella novicida (257 aa). BLAST Score = 412, Expect = e-114 Identities = 213/257 (82), Positives = 213/257 (82) Identical to FTF1708. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625. ORF ftt1353; hypothetical protein 1392636 4200569 FTF1353 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667437.1 1391863 D 393115 CDS YP_667438.1 110670881 4199234 1392676..1396662 1 NC_008245.1 Similar to AAP58966.1 PdpC from Francisella novicida (1218 aa). BLAST Score = 2172, Expect = 0.0 Identities = 1089/1220 (89), Positives = 1120/1220 (91),Gaps = 3/1220 (0) Identical to FTF1709. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1396662 pdpC1 4199234 pdpC1 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667438.1 1392676 D 393115 CDS YP_667439.1 110670882 4200570 1396680..1397255 1 NC_008245.1 Similar to AAP58977.1 (Q7X3I0) unknown protein from Francisella novicida (188 aa). BLAST Score = 321, Expect = 3e-87 Identities = 156/174 (89), Positives = 162/174 (93) Identical to FTF1710. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625 ORF ftt1355; hypothetical protein 1397255 4200570 FTF1355 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667439.1 1396680 D 393115 CDS YP_667440.1 110670883 4200659 complement(1397262..1398467) 1 NC_008245.1 Similar to AAP58965.1 (Q7C3J6) IglD from Francisella novicida (398 aa). BLAST Score = 746, Expect = 0.0 Identities = 382/398 (95), Positives = 383/398 (96) Identical to FTF1711. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus, subunit D 1398467 iglD1 4200659 iglD1 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit D YP_667440.1 1397262 R 393115 CDS YP_667441.1 110670884 4200657 complement(1398479..1399114) 1 NC_008245.1 Similar to AAP58964.1 (Q7X3J5) IglC (23 kDa protein) from Francisella novicida (211 aa). BLAST Score = 405, Expect = e-112 Identities = 209/211 (99), Positives = 210/211 (99) Identical to FTF1712. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus, subunit C 1399114 iglC1 4200657 iglC1 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit C YP_667441.1 1398479 R 393115 CDS YP_667442.1 110670885 4200655 complement(1399125..1400669) 1 NC_008245.1 Similar to AAP58963.1 (Q7X3J4) IglB from Francisella novicida (514 aa). BLAST Score = 997, Expect = 0.0 Identities = 496/514 (96), Positives = 499/514 (97) Identical to FTF1713. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus, subunit B 1400669 iglB1 4200655 iglB1 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit B YP_667442.1 1399125 R 393115 CDS YP_667443.1 110670886 4200653 complement(1400659..1401249) 1 NC_008245.1 Similar to AAP58962.1 (Q7X3J5) IglA from Francisella novicida (184 aa). BLAST Score = 350, Expect = 6e-96 Identities = 181/184 (98), Positives = 181/184 (98) Identical to FTF1714. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; intracellular growth locus, subunit A 1401249 iglA1 4200653 iglA1 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit A YP_667443.1 1400659 R 393115 CDS YP_667444.1 110670887 4199236 complement(1401294..1404881) 1 NC_008245.1 Similar to AAP58961.1 (Q7C3J6) PdpD from Francisella novicida (1245 aa). BLAST Score = 2194, Expect = 0.0 Identities = 1133/1245 (91), Positives = 1154/1245 (92), Gaps = 50/1245 (4) Identical to FTF1715. It is part of the duplicated region 1374371..1408281 which is identical to 1767715..1801625; hypothetical protein 1404881 pdpD1 4199236 pdpD1 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667444.1 1401294 R 393115 CDS YP_667445.1 110670888 4199646 complement(join(1407237..1407695,1407695..1408021)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1408021 isftu1 4199646 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667445.1 1407237 R 393115 CDS YP_667446.1 110670889 4199405 complement(1410047..1411111) 1 NC_008245.1 class II aldolase; catalyzes the reversible aldol condensation of dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in the Calvin cycle, glycolysis and gluconeogenesis; fructose-1,6-bisphosphate aldolase 1411111 fbaB 4199405 fbaB Francisella tularensis subsp. tularensis FSC198 fructose-1,6-bisphosphate aldolase YP_667446.1 1410047 R 393115 CDS YP_667447.1 110670890 4199128 complement(1411124..1412560) 1 NC_008245.1 Similar to AAO91275 (Q83AU7) Pyruvate kinase from Coxiella burnetii (477 aa). FASTA: opt: 1622 Z-score: 1687.6 E(): 3.8e-86 Smith-Waterman score: 1622; 53.249identity in 477 aa overlap domain, score 55.8,E-value 8.1e-14; pyruvate kinase 1412560 pyk 4199128 pyk Francisella tularensis subsp. tularensis FSC198 pyruvate kinase YP_667447.1 1411124 R 393115 CDS YP_667448.1 110670891 4199243 complement(1412576..1413754) 1 NC_008245.1 Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway; phosphoglycerate kinase 1413754 pgk 4199243 pgk Francisella tularensis subsp. tularensis FSC198 phosphoglycerate kinase YP_667448.1 1412576 R 393115 CDS YP_667449.1 110670892 4199272 complement(1413773..1414819) 1 NC_008245.1 Similar to Q8XKT9 Glyceraldehyde-3-phosphate dehydrogenase from Clostridium perfringens (332 aa). FASTA: opt: 1511 Z-score: 1671.9 E(): 2.8e-85 Smith-Waterman score: 1511; 71.341identity in 328 aa overlap; glyceraldehyde-3-phosphate dehydrogenase 1414819 gapA 4199272 gapA Francisella tularensis subsp. tularensis FSC198 glyceraldehyde-3-phosphate dehydrogenase YP_667449.1 1413773 R 393115 CDS YP_667450.1 110670893 4199146 complement(1414812..1416803) 1 NC_008245.1 catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase; transketolase 1416803 tktA 4199146 tktA Francisella tularensis subsp. tularensis FSC198 transketolase YP_667450.1 1414812 R 393115 CDS YP_667451.1 110670894 4199953 1416943..1417446 1 NC_008245.1 Similar to AAN42711 (Q83LI4) Orf, conserved hypothetical protein from Shigella flexneri (173 aa). FASTA: opt: 194 Z-score: 254.2 E(): 2.6e-06 Smith-Waterman score: 194; 26.708 identity in 161 aa overlap ORF ftt1370; hypothetical protein 1417446 4199953 FTF1370 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667451.1 1416943 D 393115 CDS YP_667452.1 110670895 4199224 1417490..1417672 1 NC_008245.1 some L32 proteins have zinc finger motifs consisting of CXXC while others do not; 50S ribosomal protein L32 1417672 rpmF 4199224 rpmF Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L32 YP_667452.1 1417490 D 393115 CDS YP_667453.1 110670896 4199734 1417771..1418817 1 NC_008245.1 involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY; putative glycerol-3-phosphate acyltransferase PlsX 1418817 plsX 4199734 plsX Francisella tularensis subsp. tularensis FSC198 putative glycerol-3-phosphate acyltransferase PlsX YP_667453.1 1417771 D 393115 CDS YP_667454.1 110670897 4199681 1418914..1419885 1 NC_008245.1 Similar to Q9JW56 Putative 3-oxoacyl-[acyl-carrier-protein] synthase III from Neisseria meningitidis (320 aa). FASTA: opt: 1148 Z-score: 1323.1 E(): 7.6e-66 Smith-Waterman score: 1148; 54.859 identity in 319 aa overlap; 3-oxoacyl-ACP synthase 1419885 fabH 4199681 fabH Francisella tularensis subsp. tularensis FSC198 3-oxoacyl-ACP synthase YP_667454.1 1418914 D 393115 CDS YP_667455.1 110670898 4199954 1419933..1420853 1 NC_008245.1 Similar to Q9CJS7 FabD from Pasteurella multocida (312 aa). FASTA: opt: 984 Z-score: 1099.0 E(): 2.3e-53 Smith-Waterman score: 984;50.163 identity in 307 aa overlap ORF ftt1374; malonyl CoA-acyl carrier protein transacylase 1420853 4199954 FTF1374 Francisella tularensis subsp. tularensis FSC198 malonyl CoA-acyl carrier protein transacylase YP_667455.1 1419933 D 393115 CDS YP_667456.1 110670899 4199680 1420867..1421610 1 NC_008245.1 Similar to Q9CJS6 FabG from Pasteurella multocida (242 aa). FASTA: opt: 1029 Z-score: 1192.2 E(): 1.5e-58 Smith-Waterman score: 1029; 66.667 identity in 240 aa overlap; 3-oxoacyl-(acyl-carrier-protein) reductase 1421610 fabG 4199680 fabG Francisella tularensis subsp. tularensis FSC198 3-oxoacyl-(acyl-carrier-protein) reductase YP_667456.1 1420867 D 393115 CDS YP_667457.1 110670900 4199754 1421706..1421990 1 NC_008245.1 carries the fatty acid chain in fatty acid biosynthesis; acyl carrier protein 1421990 acpP 4199754 acpP Francisella tularensis subsp. tularensis FSC198 acyl carrier protein YP_667457.1 1421706 D 393115 CDS YP_667458.1 110670901 4199679 1422070..1423329 1 NC_008245.1 Similar to Q8Y0J0 Probable 3-oxoacyl-[acyl-carrier-protein] synthase II from Ralstonia solanacearum (410 aa). FASTA: opt: 1679 Z-score: 1887.3 E(): 2.8e-97 Smith-Waterman score: 1679; 59.233 identity in 417 aa overlap; 3-oxoacyl-ACP synthase 1423329 fabF 4199679 fabF Francisella tularensis subsp. tularensis FSC198 3-oxoacyl-ACP synthase YP_667458.1 1422070 D 393115 CDS YP_667459.1 110670902 4199091 1426910..1427698 1 NC_008245.1 Similar to P22783 Inositol-1-monophosphatase from E. coli (267 aa). FASTA: opt: 630 Z-score: 759.0 E(): 2e-34 Smith-Waterman score: 656; 36.882identity in 263 aa overlap; inositol-1-monophosphatase 1427698 suhB 4199091 suhB Francisella tularensis subsp. tularensis FSC198 inositol-1-monophosphatase YP_667459.1 1426910 D 393115 CDS YP_667460.1 110670903 4199092 1427698..1428978 1 NC_008245.1 Similar to P44788 SUN protein (FMU protein) from Haemophilus influenzae (451 aa). FASTA: opt: 956 Z-score: 1079.4 E(): 2.9e-52 Smith-Waterman score: 956; 39.401 identity in 434 aa overlap; Sun protein 1428978 sun 4199092 sun Francisella tularensis subsp. tularensis FSC198 Sun protein YP_667460.1 1427698 D 393115 CDS YP_667461.1 110670904 4199959 complement(1428956..1429447) 1 NC_008245.1 Similar to Q8D9X5 Probable acetyltransferase from Vibrio vulnificus (141 aa). FASTA: opt: 183 Z-score: 242.0 E(): 1.3e-05 Smith-Waterman score: 183; 27.132 identity in 129 aa overlap ORF ftt1384c; acetyltransferase 1429447 4199959 FTF1384c Francisella tularensis subsp. tularensis FSC198 acetyltransferase YP_667461.1 1428956 R 393115 CDS YP_667462.1 110670905 4199960 complement(1429540..1429809) 1 NC_008245.1 ORF ftt1385c; hypothetical protein 1429809 4199960 FTF1385c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667462.1 1429540 R 393115 CDS YP_667463.1 110670906 4199647 join(1429800..1430126,1430126..1430584) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1430584 isftu1 4199647 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667463.1 1429800 D 393115 CDS YP_667464.1 110670907 4199553 complement(1431030..1433066) 1 NC_008245.1 Similar to P43813 DNA ligase from Haemophilus influenzae (679 aa). FASTA: opt: 2154 Z-score: 2353.2 E(): 3.2e-123 Smith-Waterman score: 2154; 48.817 identity in 676 aa overlap; DNA ligase 1433066 ligN 4199553 ligN Francisella tularensis subsp. tularensis FSC198 DNA ligase YP_667464.1 1431030 R 393115 CDS YP_667465.1 110670908 4199961 1433374..1434090 1 NC_008245.1 Similar to AAO81619 (Q833S4) Conserved domain protein from Enterococcus faecalis (281 aa). FASTA: opt: 171 Z-score: 214.0 E(): 0.00046 Smith-Waterman score: 194;26.337 identity in 243 aa overlap ORF ftt1388; hypothetical protein 1434090 4199961 FTF1388 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667465.1 1433374 D 393115 CDS YP_667466.1 110670909 4200090 1434092..1434886 1 NC_008245.1 catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate; 3-methyl-2-oxobutanoate hydroxymethyltransferase 1434886 panB 4200090 panB Francisella tularensis subsp. tularensis FSC198 3-methyl-2-oxobutanoate hydroxymethyltransferase YP_667466.1 1434092 D 393115 CDS YP_667467.1 110670910 4200091 1434873..1435658 1 NC_008245.1 Similar to AAO89975 (Q83EA3) Pantoate-beta-alanine ligase from Coxiella burnetii (257 aa). FASTA: opt: 692 Z-score: 830.8 E(): 2e-38 Smith-Waterman score: 692; 40.927 identity in 259 aa overlap; pantoate-beta-alanine ligase 1435658 panC 4200091 panC Francisella tularensis subsp. tularensis FSC198 pantoate-beta-alanine ligase YP_667467.1 1434873 D 393115 CDS YP_667468.1 110670911 4200092 1435652..1435987 1 NC_008245.1 Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5); aspartate alpha-decarboxylase 1435987 panD 4200092 panD Francisella tularensis subsp. tularensis FSC198 aspartate alpha-decarboxylase YP_667468.1 1435652 D 393115 CDS YP_667469.1 110670912 4199962 1435991..1436761 1 NC_008245.1 type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP; pantothenate kinase 1436761 4199962 FTF1392 Francisella tularensis subsp. tularensis FSC198 pantothenate kinase YP_667469.1 1435991 D 393115 CDS YP_667470.1 110670913 4200602 complement(1436770..1438638) 1 NC_008245.1 Similar to Q9RPH5 RecD from Mycobacterium smegmatis (554 aa). FASTA: opt: 593 Z-score: 627.7 E(): 4.1e-27 Smith-Waterman score: 776; 32.510 identity in 486 aa overlap; exodeoxyribonuclease V subunit alpha 1438638 recD 4200602 recD Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease V subunit alpha YP_667470.1 1436770 R 393115 CDS YP_667471.1 110670914 4200600 complement(1438604..1442257) 1 NC_008245.1 Similar to Q8EF45 Exodeoxyribonuclease V,beta subunit from Shewanella oneidensis (1259 aa). FASTA: opt: 742 Z-score: 770.7 E(): 4.5e-35 Smith-Waterman score: 1544; 30.448identity in 1317 aa overlap; exodeoxyribonuclease V subunit beta 1442257 recB 4200600 recB Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease V subunit beta YP_667471.1 1438604 R 393115 CDS YP_667472.1 110670915 4199963 complement(1442329..1443759) 1 NC_008245.1 Similar Q8A303 ATP-dependent DNA helicase from Bacteriodes thetaiotamicron (511 aa). FASTA: opt: 496 Z-score: 533.4 E(): 7.4e-22 Smith-Waterman score: 815; 32.200 identity in 500 aa overlap ORF ftt1395c; ATP-dependent DNA helicase 1443759 4199963 FTF1395c Francisella tularensis subsp. tularensis FSC198 ATP-dependent DNA helicase YP_667472.1 1442329 R 393115 CDS YP_667473.1 110670916 4199964 complement(1443902..1444480) 1 NC_008245.1 Similar to AAO81663 (Q833N3) Conserved hypothetical protein from Entercoccus faecalis (203 aa). FASTA: opt: 155 Z-score: 198.6 E(): 0.0033 Smith-Waterman score: 155; 25.850 identity in 147 aa overlap ORF ftt1396c; hypothetical protein 1444480 4199964 FTF1396c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667473.1 1443902 R 393115 CDS YP_667474.1 110670917 4200601 complement(1444416..1447655) 1 NC_008245.1 Similar to P44945 Exodeoxyribonuclease V gamma chain from Haemophilus influenzae (1121 aa). FASTA: opt: 1139 Z-score: 1214.3 E(): 8.7e-60 Smith-Waterman score: 1433; 28.344 identity in 1129 aa overlap; exodeoxyribonuclease V subunit gamma 1447655 recC 4200601 recC Francisella tularensis subsp. tularensis FSC198 exodeoxyribonuclease V subunit gamma YP_667474.1 1444416 R 393115 CDS YP_667475.1 110670918 4199966 1449494..1450363 1 NC_008245.1 Similar to Q8KH10 Conserved hypothetical integral membrane (299 aa). FASTA: opt: 516 Z-score: 612.8 E(): 2.8e-26 Smith-Waterman score: 516; 29.818 identity in 275 aa overlap ORF ftt1399; hypothetical protein 1450363 4199966 FTF1399 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667475.1 1449494 D 393115 CDS YP_667476.1 110670919 4199967 complement(1450377..1450763) 1 NC_008245.1 ORF ftt1400c; hypothetical protein 1450763 4199967 FTF1400c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667476.1 1450377 R 393115 CDS YP_667477.1 110670920 4199968 1450920..1451585 1 NC_008245.1 Similar to Q8HA35 Repressor (217 aa). FASTA: opt: 183 Z-score: 217.7 E(): 0.00028 Smith-Waterman score: 225; 29.167 identity in 216 aa overlap ORF ftt1401; prophage repressor protein 1451585 4199968 FTF1401 Francisella tularensis subsp. tularensis FSC198 prophage repressor protein YP_667477.1 1450920 D 393115 CDS YP_667478.1 110670921 4199761 complement(1452157..1453791) 1 NC_008245.1 ORF ftt1402c; hypothetical protein 1453791 4199761 FTF1402c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667478.1 1452157 R 393115 CDS YP_667479.1 110670922 4199408 complement(1453808..1454032) 1 NC_008245.1 Similar to P33649 Ferrous iron transport protein A from E. coli (75 aa). FASTA: opt: 148 Z-score: 228.5 E(): 7.1e-05 Smith-Waterman score: 148; 31.081 identity in 74 aa overlap; ferrous iron transport protein A 1454032 feoA 4199408 feoA Francisella tularensis subsp. tularensis FSC198 ferrous iron transport protein A YP_667479.1 1453808 R 393115 CDS YP_667480.1 110670923 4199762 1454141..1454848 1 NC_008245.1 Similar to Q8E9Y6 Cell division protein FtsN from Shewanella oneidensis (231 aa). FASTA: opt: 157 Z-score: 187.2 E(): 0.014 Smith-Waterman score: 180; 27.094 identity in 203 aa overlap ORF ftt1404; cell division protein 1454848 4199762 FTF1404 Francisella tularensis subsp. tularensis FSC198 cell division protein YP_667480.1 1454141 D 393115 CDS YP_667481.1 110670924 4199792 complement(1454926..1455684) 1 NC_008245.1 Similar to Q47677 Probable hydroxyacylglutathione hydrolase from E. coli (251 aa). FASTA: opt: 426 Z-score: 519.2 E(): 4.6e-21 Smith-Waterman score: 426; 36.290 identity in 248 aa overlap; hydroxyacylglutathione hydrolase 1455684 gloB 4199792 gloB Francisella tularensis subsp. tularensis FSC198 hydroxyacylglutathione hydrolase YP_667481.1 1454926 R 393115 CDS YP_667482.1 110670925 4199763 complement(1455698..1456855) 1 NC_008245.1 ORF ftt1406c; hypothetical protein 1456855 4199763 FTF1406c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667482.1 1455698 R 393115 CDS YP_667483.1 110670926 4199764 complement(1456852..1457937) 1 NC_008245.1 ORF ftt1407c; hypothetical protein 1457937 4199764 FTF1407c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667483.1 1456852 R 393115 CDS YP_667484.1 110670927 4200373 complement(1457903..1458652) 1 NC_008245.1 Similar to Q980U3 Uroporphyrinogen III synthase,putative from Sulfolobus solfataricus (237 aa). FASTA: opt: 171 Z-score: 213.0 E(): 0.00052 Smith-Waterman score: 171; 34.746 identity in 118 aa overlap; uroporphyrinogen III synthase 1458652 hemD 4200373 hemD Francisella tularensis subsp. tularensis FSC198 uroporphyrinogen III synthase YP_667484.1 1457903 R 393115 CDS YP_667485.1 110670928 4199765 complement(1458653..1459069) 1 NC_008245.1 Similar to Q8PK22 Hypothetical protein from Xanthomonas axonopodis (145 aa). FASTA: opt: 415 Z-score: 523.5 E(): 2.6e-21 Smith-Waterman score: 415; 45.652 identity in 138 aa overlap ORF ftt1409c; hypothetical protein 1459069 4199765 FTF1409c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667485.1 1458653 R 393115 CDS YP_667486.1 110670929 4199766 1459767..1460312 1 NC_008245.1 Similar to Q8XTB4 Probable transmembrane protein from Ralstonia solanacearum (184 aa). FASTA: opt: 443 Z-score: 558.2 E(): 3e-23 Smith-Waterman score: 443; 42.045 identity in 176 aa overlap ORF ftt1410; hypothetical protein 1460312 4199766 FTF1410 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667486.1 1459767 D 393115 CDS YP_667487.1 110670930 4199944 1462901..1464598 1 NC_008245.1 catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro); prolyl-tRNA synthetase 1464598 proS 4199944 proS Francisella tularensis subsp. tularensis FSC198 prolyl-tRNA synthetase YP_667487.1 1462901 D 393115 CDS YP_667488.1 110670931 4199768 1464744..1465874 1 NC_008245.1 Similar to Q886Y5 Aminotransferase, classes I and II from Pseudomonas syringae (382 aa). FASTA: opt: 929 Z-score: 1036.9 E(): 6.6e-50 Smith-Waterman score: 929; 37.076 identity in 383 aa overlap ORF ftt1413; aminotransferase 1465874 4199768 FTF1413 Francisella tularensis subsp. tularensis FSC198 aminotransferase YP_667488.1 1464744 D 393115 CDS YP_667489.1 110670932 4199769 1465874..1466356 1 NC_008245.1 ORF ftt1414; hypothetical protein 1466356 4199769 FTF1414 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667489.1 1465874 D 393115 CDS YP_667490.1 110670933 4199770 1466464..1467366 1 NC_008245.1 ORF ftt1415; hypothetical protein 1467366 4199770 FTF1415 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667490.1 1466464 D 393115 CDS YP_667491.1 110670934 4199771 complement(1467418..1467831) 1 NC_008245.1 ORF ftt1416c; lipoprotein 1467831 4199771 FTF1416c Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667491.1 1467418 R 393115 CDS YP_667492.1 110670935 4199772 1467985..1468770 1 NC_008245.1 Similar to Q8ZRJ8 Putative amidohydrolase from Salmonella typhimurium (255 aa). FASTA: opt: 723 Z-score: 882.8 E(): 2.6e-41 Smith-Waterman score: 723; 43.359 identity in 256 aa overlap ORF ftt1417; carbon-nitrogen hydrolase 1468770 4199772 FTF1417 Francisella tularensis subsp. tularensis FSC198 carbon-nitrogen hydrolase YP_667492.1 1467985 D 393115 CDS YP_667493.1 110670936 4200758 complement(1468754..1469179) 1 NC_008245.1 Similar to Q889Q5 N utilization substance protein B from Pseudomonas syringae (165 aa). FASTA: opt: 274 Z-score: 347.8 E(): 1.6e-11 Smith-Waterman score: 274; 32.847 identity in 137 aa overlap; N utilisation substance protein B 1469179 nusB 4200758 nusB Francisella tularensis subsp. tularensis FSC198 N utilisation substance protein B YP_667493.1 1468754 R 393115 CDS YP_667494.1 110670937 4199773 1469366..1469692 1 NC_008245.1 ORF ftt1419; lipoprotein 1469692 4199773 FTF1419 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667494.1 1469366 D 393115 CDS YP_667495.1 110670938 4199774 1469825..1470292 1 NC_008245.1 ORF ftt1420; hypothetical protein 1470292 4199774 FTF1420 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667495.1 1469825 D 393115 CDS YP_667496.1 110670939 4199775 1470511..1470960 1 NC_008245.1 Similar to Q88E79 Conserved hypothetical protein from Pseudomonas putida (150 aa). FASTA: opt: 511 Z-score: 638.2 E(): 1.1e-27 Smith-Waterman score: 511; 46.809 identity in 141 aa overlap ORF ftt1421; hypothetical protein 1470960 4199775 FTF1421 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667496.1 1470511 D 393115 CDS YP_667497.1 110670940 4199776 1471000..1471620 1 NC_008245.1 Similar to Q87UK0 Oxidoreductase, 2OG-Fe(II) oxygenase family (244 aa). FASTA: opt: 400 Z-score: 493.2 E(): 1.3e-19 33.163 identity in 196 aa overlap ORF ftt1422; oxidoreductase 1471620 4199776 FTF1422 Francisella tularensis subsp. tularensis FSC198 oxidoreductase YP_667497.1 1471000 D 393115 CDS YP_667498.1 110670941 4199777 complement(1471613..1472362) 1 NC_008245.1 Similar to Q8NQW1 Predicted permease from Corynebacterium glutamicum (250 aa). FASTA: opt: 472 Z-score: 553.4 E(): 5.6e-23 Smith-Waterman score: 472; 35.573 identity in 253 aa overlap. ORF ftt1423c; hypothetical protein 1472362 4199777 FTF1423c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667498.1 1471613 R 393115 CDS YP_667499.1 110670942 4199778 complement(1472364..1473044) 1 NC_008245.1 Similar to Q89VN8 Bll1007 protein from Bradyrhizobium japonicum (345 aa). FASTA: opt: 834 Z-score: 1018.3 E(): 7.2e-49 Smith-Waterman score: 834; 53.509 identity in 228 aa overlap ORF ftt1424c; hypothetical protein 1473044 4199778 FTF1424c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667499.1 1472364 R 393115 CDS YP_667500.1 110670943 4200235 complement(1473089..1474783) 1 NC_008245.1 Similar to Q8XMF5 NADH oxidase from Clostridium perfringens (566 aa). FASTA: opt: 2055 Z-score: 2177.9 E(): 1.8e-113 57.117 identity in 548 aa overlap; NADH oxidase 1474783 naoX 4200235 naoX Francisella tularensis subsp. tularensis FSC198 NADH oxidase YP_667500.1 1473089 R 393115 CDS YP_667501.1 110670944 4199779 complement(1474805..1475323) 1 NC_008245.1 Similar to Q8YQC8 Hypothetical protein from Anabaena sp. (177 aa). FASTA: opt: 465 Z-score: 572.2 E(): 5e-24 Smith-Waterman score: 465; 43.023 identity in 172 aa overlap ORF ftt1426c; hypothetical protein 1475323 4199779 FTF1426c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667501.1 1474805 R 393115 CDS YP_667502.1 110670945 4200173 join(1475863..1476189,1476189..1476647) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1476647 isftu1 4200173 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667502.1 1475863 D 393115 CDS YP_667503.1 110670946 4199780 complement(1476808..1477323) 1 NC_008245.1 Similar to AAP10414 (Q81AS7) Acetyltransferase from Bacillus cereus (174 aa). FASTA: opt: 372 Z-score: 461.6 E(): 7.3e-18 Smith-Waterman score: 372; 40.341 identity in 176 aa overlap ORF ftt1428c; acetyltransferase 1477323 4199780 FTF1428c Francisella tularensis subsp. tularensis FSC198 acetyltransferase YP_667503.1 1476808 R 393115 CDS YP_667504.1 110670947 4199212 1478503..1479138 1 NC_008245.1 Similar Q8DDM9 to Putative threonine efflux protein from Vibrio vulnificus (223 aa). FASTA: opt: 310 Z-score: 386.3 E(): 1.1e-13 Smith-Waterman score: 310; 29.577 identity in 213 aa overlap; threonine efflux protein 1479138 rhtC 4199212 rhtC Francisella tularensis subsp. tularensis FSC198 threonine efflux protein YP_667504.1 1478503 D 393115 CDS YP_667505.1 110670948 4199935 complement(1479135..1480025) 1 NC_008245.1 Similar to Q89AR9 Putative inorganic polyphosphate/ATP-NAD kinase from Buchnera aphidicola (292 aa). FASTA: opt: 682 Z-score: 800.3 E(): 1e-36 Smith-Waterman score: 682; 38.776 identity in 294 aa overlap; inorganic phosphate/ATP-NAD kinase 1480025 ppnK 4199935 ppnK Francisella tularensis subsp. tularensis FSC198 inorganic phosphate/ATP-NAD kinase YP_667505.1 1479135 R 393115 CDS YP_667506.1 110670949 4200364 1480214..1481158 1 NC_008245.1 Similar to Q55487 Putative glycosyl transferase from Synechocystis sp. (318 aa). FASTA: opt: 1079 Z-score: 1283.0 E(): 1.3e-63 Smith-Waterman score: 1079; 50.000 identity in 298 aa overlap; glycosyl transferase family protein 1481158 gtrB 4200364 gtrB Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_667506.1 1480214 D 393115 CDS YP_667507.1 110670950 4200697 complement(1481162..1481941) 1 NC_008245.1 Similar Q88LN3 ABC transporter,permease protein from Pseudomonas putida (259 aa). FASTA: opt: 890 Z-score: 979.2 E(): 1.1e-46 Smith-Waterman score: 890; 50.193 identity in 259 aa overlap; ABC transporter membrane protein 1481941 yadH 4200697 yadH Francisella tularensis subsp. tularensis FSC198 ABC transporter membrane protein YP_667507.1 1481162 R 393115 CDS YP_667508.1 110670951 4200698 complement(1481950..1482873) 1 NC_008245.1 Similar to Q9CNG2 YagD from Pasteurella multocida (312 aa). FASTA: opt: 1028 Z-score: 1082.3 E(): 2e-52 Smith-Waterman score: 1028; 50.662 identity in 302 aa overlap; ABC transporter ATP-binding protein 1482873 yagD 4200698 yagD Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_667508.1 1481950 R 393115 CDS YP_667509.1 110670952 4199295 1483056..1483799 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 1483799 isftu2 4199295 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_667509.1 1483056 D 393115 CDS YP_667510.1 110670953 4200066 complement(1485078..1485845) 1 NC_008245.1 Similar to Q87N28 Putative deoxyribonuclease from vibrio parahaemolyticus (255 aa). FASTA: opt: 787 Z-score: 917.1 E(): 3.1e-43 Smith-Waterman score: 787; 44.314 identity in 255 aa overlap ORF ftt1439c; deoxyribonuclease 1485845 4200066 FTF1439c Francisella tularensis subsp. tularensis FSC198 deoxyribonuclease YP_667510.1 1485078 R 393115 CDS YP_667511.1 110670954 4200068 1487511..1487996 1 NC_008245.1 Similar to Q8YQ94 Hypothetical protein from Anabaena sp. (144 aa). FASTA: opt: 497 Z-score: 629.3 E(): 3.4e-27 Smith-Waterman score: 497; 54.610 identity in 141 aa overlap ORF ftt1441; hypothetical protein 1487996 4200068 FTF1441 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667511.1 1487511 D 393115 CDS YP_667512.1 110670955 4200327 complement(1488614..1489570) 1 NC_008245.1 catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme; DNA-directed RNA polymerase subunit alpha 1489570 rpoA2 4200327 rpoA2 Francisella tularensis subsp. tularensis FSC198 DNA-directed RNA polymerase subunit alpha YP_667512.1 1488614 R 393115 CDS YP_667513.1 110670956 4200069 complement(1489658..1490560) 1 NC_008245.1 Similar to Q87ZS7 ATPase, AAA family from Pseudomonas syringae (440 aa). FASTA: opt: 1018 Z-score: 1104.6 E(): 1.1e-53 Smith-Waterman score: 1096; 43.243 identity in 407 aa overlap ORF ftt1443c; recombination factor protein RarA 1490560 4200069 FTF1443c Francisella tularensis subsp. tularensis FSC198 recombination factor protein RarA YP_667513.1 1489658 R 393115 CDS YP_667514.1 110670957 4199936 complement(1491128..1492063) 1 NC_008245.1 Similar to P44828 Putative exopolyphosphatase from Pneumophilus influenzae (323 aa). FASTA: opt: 730 Z-score: 856.9 E(): 7.1e-40 Smith-Waterman score: 730; 41.275 identity in 298 aa overlap; exopolyphosphatase 1492063 ppx 4199936 ppx Francisella tularensis subsp. tularensis FSC198 exopolyphosphatase YP_667514.1 1491128 R 393115 CDS YP_667515.1 110670958 4198969 1492252..1492575 1 NC_008245.1 Similar to Q9JYY9 Thioredoxin from Neisseria meningitidis (110 aa). FASTA: opt: 452 Z-score: 595.9 E(): 2.4e-25 Smith-Waterman score: 452; 62.000 identity in 100 aa overlap; thioredoxin 1492575 trxA1 4198969 trxA1 Francisella tularensis subsp. tularensis FSC198 thioredoxin YP_667515.1 1492252 D 393115 CDS YP_667516.1 110670959 4199211 1492618..1493880 1 NC_008245.1 An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes; transcription termination factor Rho 1493880 rho 4199211 rho Francisella tularensis subsp. tularensis FSC198 transcription termination factor Rho YP_667516.1 1492618 D 393115 CDS YP_667517.1 110670960 4199659 complement(1494992..1496476) 1 NC_008245.1 Similar to Q8UCV4 Phosphomannomutase from Agrobacterium tumefaciens (506 aa). FASTA: opt: 1226 Z-score: 1345.0 E(): 4.6e-67 Smith-Waterman score: 1226; 41.127 identity in 479 aa overlap; phosphomannomutase 1496476 manB 4199659 manB Francisella tularensis subsp. tularensis FSC198 phosphomannomutase YP_667517.1 1494992 R 393115 CDS YP_667518.1 110670961 4199660 complement(1496507..1497913) 1 NC_008245.1 Similar to Q9ETR3 GDP-mannose pyrophosphorylase from Salmonella enterica (464 aa). FASTA: opt: 1571 Z-score: 1720.2 E(): 5.8e-88 Smith-Waterman score: 1571; 50.647 identity in 464 aa overlap; mannose-1-phosphate guanyltransferase 1497913 manC 4199660 manC Francisella tularensis subsp. tularensis FSC198 mannose-1-phosphate guanyltransferase YP_667518.1 1496507 R 393115 CDS YP_667519.1 110670962 4200687 complement(1498880..1499926) 1 NC_008245.1 Similar to Q9RDY1 RmlB protein from Legionella pneumophila (351 aa). FASTA: opt: 1490 Z-score: 1729.8 E(): 1.7e-88 Smith-Waterman score: 1490; 64.392 identity in 337 aa overlap; dTDP-D-glucose 4,6-dehydratase 1499926 wbtM 4200687 wbtM Francisella tularensis subsp. tularensis FSC198 dTDP-D-glucose 4,6-dehydratase YP_667519.1 1498880 R 393115 CDS YP_667520.1 110670963 4200152 complement(1500443..1501327) 1 NC_008245.1 Similar to O66250 Glucose-1-phosphate-thymidylyltransferase from Actinobacillus actinomycetemcomitans (290 aa). FASTA: opt: 1336 Z-score: 1567.4 E(): 1.9e-79 Smith-Waterman score: 1336; 67.466 identity in 292 aa overlap; glucose-1-phosphate thymidylyltransferase 1501327 wbtL 4200152 wbtL Francisella tularensis subsp. tularensis FSC198 glucose-1-phosphate thymidylyltransferase YP_667520.1 1500443 R 393115 CDS YP_667521.1 110670964 4200151 complement(1501352..1502212) 1 NC_008245.1 Similar to Q8ECG2 Glycosyl transferase, group 2 family protein from Shewanella oneidensis (298 aa). FASTA: opt: 724 Z-score: 845.9 E(): 2.9e-39 Smith-Waterman score: 724; 39.789 identity in 284 aa overlap; glycosyltransferase 1502212 wbtK 4200151 wbtK Francisella tularensis subsp. tularensis FSC198 glycosyltransferase YP_667521.1 1501352 R 393115 CDS YP_667522.1 110670965 4200690 complement(1502205..1503692) 1 NC_008245.1 Similar to AAO39697(Q84DG4) O antigen flippase from E. coli (464 aa). FASTA: opt: 861 Z-score: 954.5 E(): 2.6e-45 Smith-Waterman score: 861; 31.343identity in 469 aa overlap; O-antigen flippase 1503692 wzx 4200690 wzx Francisella tularensis subsp. tularensis FSC198 O-antigen flippase YP_667522.1 1502205 R 393115 CDS YP_667523.1 110670966 4200150 complement(1503697..1504422) 1 NC_008245.1 Similar to Q50721 Hypothetical protein Rv3404c precurser from Mycobacterium tuberculosis (234 aa). FASTA: opt: 562 Z-score: 656.5 E(): 1e-28 Smith-Waterman score: 562; 43.243 identity in 185 aa overlap; hypothetical protein 1504422 wbtJ 4200150 wbtJ Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667523.1 1503697 R 393115 CDS YP_667524.1 110670967 4200149 complement(1504419..1505501) 1 NC_008245.1 Similarity to Q97GL6 Sugar transaminase, involved in dTDP-4-amino-4,6-dideoxyglucose biosynthesis from Clostridium acetobutylicum (364 aa). FASTA: opt: 1344 Z-score: 1521.8 E(): 6.5e-77 Smith-Waterman score: 1344; 59.155 identity in 355 aa overlap; sugar transamine/perosamine synthetase 1505501 wbtI 4200149 wbtI Francisella tularensis subsp. tularensis FSC198 sugar transamine/perosamine synthetase YP_667524.1 1504419 R 393115 CDS YP_667525.1 110670968 4200148 complement(1505494..1507380) 1 NC_008245.1 Similar to Q8GGB6 Asparagine synthase-like protein from Pseudomonas aeruginosa (627 aa). FASTA: opt: 1855 Z-score: 2052.2 E(): 1.9e-106 Smith-Waterman score: 1855; 46.022 identity in 641 aa overlap; asparagine synthase 1507380 wbtH 4200148 wbtH Francisella tularensis subsp. tularensis FSC198 asparagine synthase YP_667525.1 1505494 R 393115 CDS YP_667526.1 110670969 4200147 complement(1507380..1508480) 1 NC_008245.1 Similar to Q8ECG6 Glycosyl transferase group 1 family protein from Shewanella oneidensis (373 aa). FASTA: opt: 531 Z-score: 625.5 E(): 5.5e-27 Smith-Waterman score: 531; 32.353 identity in 374 aa overlap; glycosyl transferase family protein 1508480 wbtG 4200147 wbtG Francisella tularensis subsp. tularensis FSC198 glycosyl transferase family protein YP_667526.1 1507380 R 393115 CDS YP_667527.1 110670970 4200691 complement(1508477..1509706) 1 NC_008245.1 membrane protein/O-antigen polymerase (personal communication from authors); membrane protein/O-antigen protein 1509706 wzy 4200691 wzy Francisella tularensis subsp. tularensis FSC198 membrane protein/O-antigen protein YP_667527.1 1508477 R 393115 CDS YP_667528.1 110670971 4200146 complement(1509723..1510694) 1 NC_008245.1 Similar to Q8KN66 WbpP from Pseudomonas aeruginosa (341 aa). FASTA: opt: 1033 Z-score: 1225.7 E(): 2e-60 Smith-Waterman score: 1033; 51.562identity in 320 aa overlap; NAD dependent epimerase 1510694 wbtF 4200146 wbtF Francisella tularensis subsp. tularensis FSC198 NAD dependent epimerase YP_667528.1 1509723 R 393115 CDS YP_667529.1 110670972 4200145 complement(1510694..1512004) 1 NC_008245.1 Similar to Q8EMC3 Vi polysaccharide biosynthesis from Oceanobacillus iheyensis (437 aa). FASTA: opt: 1817 Z-score: 2048.6 E(): 2.9e-106 Smith-Waterman score: 1817; 59.268 identity in 437 aa overlap; UDP-glucose/GDP-mannose dehydrogenase 1512004 wbtE 4200145 wbtE Francisella tularensis subsp. tularensis FSC198 UDP-glucose/GDP-mannose dehydrogenase YP_667529.1 1510694 R 393115 CDS YP_667530.1 110670973 4200144 complement(1512022..1513113) 1 NC_008245.1 Similar to Q8EMF7 Galacturonosyl transferase from Oceanobacillus iheyensis (362 aa). FASTA: opt: 908 Z-score: 1050.2 E(): 1.2e-50 Smith-Waterman score: 908; 40.223 identity in 358 aa overlap; galacturonosyl transferase 1513113 wbtD 4200144 wbtD Francisella tularensis subsp. tularensis FSC198 galacturonosyl transferase YP_667530.1 1512022 R 393115 CDS YP_667531.1 110670974 4200143 complement(1513103..1513894) 1 NC_008245.1 Similar to Q9K6L4 UDP-glucose 4-epimerase from Bacillus halodurans (282 aa). FASTA: opt: 758 Z-score: 904.3 E(): 1.6e-42 Smith-Waterman score: 758; 45.455 identity in 264 aa overlap; UDP-glucose 4-epimerase 1513894 wbtC 4200143 wbtC Francisella tularensis subsp. tularensis FSC198 UDP-glucose 4-epimerase YP_667531.1 1513103 R 393115 CDS YP_667532.1 110670975 4200142 complement(1513896..1514513) 1 NC_008245.1 Similarity to Q9K6L3 Galactosyltransferase from Bacillus halodurans (207 aa). FASTA: opt: 902 Z-score: 1122.0 E(): 1.2e-54 Smith-Waterman score: 902; 66.667 identity in 195 aa overlap; galactosyl transferase 1514513 wbtB 4200142 wbtB Francisella tularensis subsp. tularensis FSC198 galactosyl transferase YP_667532.1 1513896 R 393115 CDS YP_667533.1 110670976 4200141 complement(1514521..1516257) 1 NC_008245.1 Similar to Q9S4V4 PglF from Campylobacter jejuni (590 aa). FASTA: opt: 1343 Z-score: 1435.9 E(): 3.9e-72 Smith-Waterman score: 1378; 43.543identity in 604 aa overlap; dTDP-glucose 4,6-dehydratase 1516257 wbtA 4200141 wbtA Francisella tularensis subsp. tularensis FSC198 dTDP-glucose 4,6-dehydratase YP_667533.1 1514521 R 393115 CDS YP_667534.1 110670977 4199296 complement(1516359..1517102) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 1517102 isftu2 4199296 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_667534.1 1516359 R 393115 CDS YP_667535.1 110670978 4200230 complement(1518207..1519694) 1 NC_008245.1 Similar to Q8XQG4 L-aspartate oxidase 2 from Ralstonia solanacearum (536 aa). FASTA: opt: 1043 Z-score: 1161.3 E(): 7.8e-57 Smith-Waterman score: 1043; 38.008 identity in 492 aa overlap; L-aspartate oxidase 1519694 nadB 4200230 nadB Francisella tularensis subsp. tularensis FSC198 L-aspartate oxidase YP_667535.1 1518207 R 393115 CDS YP_667536.1 110670979 4200231 complement(1519697..1520560) 1 NC_008245.1 Similar to Q8DC25 Nicotinate-nucleotide pyrophosphorylase from Vibrio vulnificus (295 aa). FASTA: opt: 888 Z-score: 999.5 E(): 8e-48 Smith-Waterman score: 888; 53.737 identity in 281 aa overlap; nicotinate-nucleotide pyrophosphorylase 1520560 nadC 4200231 nadC Francisella tularensis subsp. tularensis FSC198 nicotinate-nucleotide pyrophosphorylase YP_667536.1 1519697 R 393115 CDS YP_667537.1 110670980 4200117 complement(1520569..1521594) 1 NC_008245.1 3 different subfamilies; catalyzes the formation of quinolinate from iminoaspartate and dihydroxyacetone phosphate; quinolinate synthetase 1521594 nadA 4200117 nadA Francisella tularensis subsp. tularensis FSC198 quinolinate synthetase YP_667537.1 1520569 R 393115 CDS YP_667538.1 110670981 4200291 complement(1521615..1522187) 1 NC_008245.1 Essential for recycling GMP and indirectly, cGMP; guanylate kinase 1522187 gmk 4200291 gmk Francisella tularensis subsp. tularensis FSC198 guanylate kinase YP_667538.1 1521615 R 393115 CDS YP_667539.1 110670982 4199531 complement(1522304..1524013) 1 NC_008245.1 Similar to DEAD_KLEPN (P33906) Cold-shock DEAD-box protein A (ATP-dependent RNA helicase deaD) from Klebsiella pneumoniae (642 aa). FASTA: opt: 1669 Z-score: 1759.9 E(): 3.5e-90 Smith-Waterman score: 1669; 45.070 identity in 568 aa overlap; cold-shock DEAD-box protein A 1524013 deaD 4199531 deaD Francisella tularensis subsp. tularensis FSC198 cold-shock DEAD-box protein A YP_667539.1 1522304 R 393115 CDS YP_667540.1 110670983 4199934 complement(1524078..1524356) 1 NC_008245.1 Identical to Q9ETL6 Putative parvulin (Putative peptidyl-prolyl cis-trans isomerase) from Francisella tularensis ssp. tularensis strain SchuS4 (FSC237) (92 aa).; peptidyl-prolyl cis-trans isomerase 1524356 ppiC 4199934 ppiC Francisella tularensis subsp. tularensis FSC198 peptidyl-prolyl cis-trans isomerase YP_667540.1 1524078 R 393115 CDS YP_667541.1 110670984 4199269 complement(1524451..1525845) 1 NC_008245.1 Similar to GALP__ECOLI Galactose-proton symporter from E. coli (464 aa). FASTA: opt: 965 Z-score: 1103.1 E(): 1.4e-53 Smith-Waterman score: 965; 36.226 identity in 461 aa overlap; major facilitator superfamily galactose-proton symporter 1525845 galP2 4199269 galP2 Francisella tularensis subsp. tularensis FSC198 major facilitator superfamily galactose-proton symporter YP_667541.1 1524451 R 393115 CDS YP_667542.1 110670985 4199268 complement(1525877..1527259) 1 NC_008245.1 Similar to AAO70555 (Q8XEV6) Galactose-proton symport from Salmonella typhimurium (464 aa). FASTA: opt: 1295 Z-score: 1483.7 E(): 8.6e-75 Smith-Waterman score: 1295; 45.140 identity in 463 aa overlap; major facilitator superfamily galactose-proton symporter 1527259 galP1 4199268 galP1 Francisella tularensis subsp. tularensis FSC198 major facilitator superfamily galactose-proton symporter YP_667542.1 1525877 R 393115 CDS YP_667543.1 110670986 4199270 1527350..1528375 1 NC_008245.1 catalyzes the interconversion of UDP-galactose and galactose-1-P with UDP-galactose and glucose-1-P; galactose-1-phosphate uridylyltransferase 1528375 galT 4199270 galT Francisella tularensis subsp. tularensis FSC198 galactose-1-phosphate uridylyltransferase YP_667543.1 1527350 D 393115 CDS YP_667544.1 110670987 4199266 1528378..1529526 1 NC_008245.1 Similar to GAL1_ACTPL Galactokinase from Actinobacillus pleuropneumoniae (384 aa) FASTA: opt: 959 Z-score: 1098.0 E(): 2.6e-53 Smith-Waterman score: 959; 38.710identity in 372 aa overlap; galactokinase 1529526 galK 4199266 galK Francisella tularensis subsp. tularensis FSC198 galactokinase YP_667544.1 1528378 D 393115 CDS YP_667545.1 110670988 4200070 complement(1529523..1529918) 1 NC_008245.1 Similar to Q88NK0 Conserved hypothetical protein from Pseudomonas putida (139 aa). FASTA: opt: 362 Z-score: 463.4 E(): 5.9e-18 Smith-Waterman score: 362; 40.458 identity in 131 aa overlap ORF ftt1477c; hypothetical protein 1529918 4200070 FTF1477c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667545.1 1529523 R 393115 CDS YP_667546.1 110670989 4199507 complement(1529911..1530663) 1 NC_008245.1 CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS; 3-deoxy-manno-octulosonate cytidylyltransferase 1530663 kdsB 4199507 kdsB Francisella tularensis subsp. tularensis FSC198 3-deoxy-manno-octulosonate cytidylyltransferase YP_667546.1 1529911 R 393115 CDS YP_667547.1 110670990 4200071 complement(1530656..1530844) 1 NC_008245.1 Similarity to Q8DAV0 Conserved hypothetical protein from Vibrio vulnificus (59 aa). FASTA: opt: 252 Z-score: 369.5 E(): 1e-12 Smith-Waterman score: 252; 56.140 identity in 57 aa overlap ORF ftt1479c; hypothetical protein 1530844 4200071 FTF1479c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667547.1 1530656 R 393115 CDS YP_667548.1 110670991 4200072 complement(1531229..1531408) 1 NC_008245.1 ORF ftt1480c; hypothetical protein 1531408 4200072 FTF1480c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667548.1 1531229 R 393115 CDS YP_667549.1 110670992 4200175 complement(join(1531569..1532027,1532027..1532353)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1532353 isftu1 4200175 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667549.1 1531569 R 393115 CDS YP_667550.1 110670993 4199565 complement(1532726..1534138) 1 NC_008245.1 Similar to AAO90013 (Q83E67) Dihydrolipoamide dehydrogenase from Coxiella burnetti (474 aa). FASTA: opt: 2052 Z-score: 2129.3 E(): 9.5e-111 Smith-Waterman score: 2052; 65.890 identity in 472 aa overlap.; dihydrolipoamide dehydrogenase 1534138 lpd 4199565 lpd Francisella tularensis subsp. tularensis FSC198 dihydrolipoamide dehydrogenase YP_667550.1 1532726 R 393115 CDS YP_667551.1 110670994 4199751 complement(1534152..1536047) 1 NC_008245.1 E2 component of pyruvate dehydrogenase multienzyme complex; in Escherichia coli AceF contains three N-terminal lipoyl domains; dihydrolipoamide acetyltransferase 1536047 aceF 4199751 aceF Francisella tularensis subsp. tularensis FSC198 dihydrolipoamide acetyltransferase YP_667551.1 1534152 R 393115 CDS YP_667552.1 110670995 4199750 complement(1536065..1538746) 1 NC_008245.1 E1 component; part of pyruvate dehydrogenase; forms a complex with DlaT and LpdC; pyruvate dehydrogenase subunit E1 1538746 aceE 4199750 aceE Francisella tularensis subsp. tularensis FSC198 pyruvate dehydrogenase subunit E1 YP_667552.1 1536065 R 393115 CDS YP_667553.1 110670996 4200074 complement(1539046..1540182) 1 NC_008245.1 Similar to AAO91321 (Q83AQ3) Hypothetical protein from Coxiella burnetti (388 aa). FASTA: opt: 858 Z-score: 1007.0 E(): 3.1e-48 Smith-Waterman score: 858 38.504 identity in 361 aa overlap ORF ftt1486c; hypothetical protein 1540182 4200074 FTF1486c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667553.1 1539046 R 393115 CDS YP_667554.1 110670997 4200271 1540310..1540924 1 NC_008245.1 catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis; dephospho-CoA kinase 1540924 coaE 4200271 coaE Francisella tularensis subsp. tularensis FSC198 dephospho-CoA kinase YP_667554.1 1540310 D 393115 CDS YP_667555.1 110670998 4200723 1541221..1542225 1 NC_008245.1 catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; tryptophanyl-tRNA synthetase 1542225 trpS 4200723 trpS Francisella tularensis subsp. tularensis FSC198 tryptophanyl-tRNA synthetase YP_667555.1 1541221 D 393115 CDS YP_667556.1 110670999 4200075 1542227..1542952 1 NC_008245.1 ORF ftt1489; hypothetical protein 1542952 4200075 FTF1489 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667556.1 1542227 D 393115 CDS YP_667557.1 110671000 4200076 1542962..1544260 1 NC_008245.1 Similar to Q9RZK7 NA+/H+ antiporter, putative, from Deinococcus radiodurans (422 aa). FASTA: opt: 529 Z-score: 612.1 E(): 3.1e-26 Smith-Waterman score: 771; 36.211 identity in 417 aa overlap ORF ftt1490; Na+/H+ antiporter 1544260 4200076 FTF1490 Francisella tularensis subsp. tularensis FSC198 Na+/H+ antiporter YP_667557.1 1542962 D 393115 CDS YP_667558.1 110671001 4200077 complement(1544659..1544973) 1 NC_008245.1 ORF ftt1491c; hypothetical protein 1544973 4200077 FTF1491c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667558.1 1544659 R 393115 CDS YP_667559.1 110671002 4200078 complement(1545069..1545695) 1 NC_008245.1 ORF ftt1492c; hypothetical protein 1545695 4200078 FTF1492c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667559.1 1545069 R 393115 CDS YP_667560.1 110671003 4200079 complement(1546185..1546580) 1 NC_008245.1 ORF ftt1493c; hypothetical protein 1546580 4200079 FTF1493c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667560.1 1546185 R 393115 CDS YP_667561.1 110671004 4199875 complement(1546582..1547466) 1 NC_008245.1 catalyzes the cleavage of the lactyl ether moiety of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate; involved in MurNAc dissimilation pathway; N-acetylmuramic acid 6-phosphate etherase 1547466 murQ 4199875 murQ Francisella tularensis subsp. tularensis FSC198 N-acetylmuramic acid 6-phosphate etherase YP_667561.1 1546582 R 393115 CDS YP_667562.1 110671005 4199876 complement(1547463..1549427) 1 NC_008245.1 ORF ftt1495c; hypothetical protein 1549427 4199876 FTF1495c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667562.1 1547463 R 393115 CDS YP_667563.1 110671006 4199877 complement(1549436..1550449) 1 NC_008245.1 ORF ftt1496c; hypothetical protein 1550449 4199877 FTF1496c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667563.1 1549436 R 393115 CDS YP_667564.1 110671007 4199878 complement(1550430..1550933) 1 NC_008245.1 ORF ftt1497c; hypothetical protein 1550933 4199878 FTF1497c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667564.1 1550430 R 393115 CDS YP_667565.1 110671008 4199746 complement(1550953..1551900) 1 NC_008245.1 catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein; acetyl-CoA carboxylase carboxyltransferase subunit alpha 1551900 accA 4199746 accA Francisella tularensis subsp. tularensis FSC198 acetyl-CoA carboxylase carboxyltransferase subunit alpha YP_667565.1 1550953 R 393115 CDS YP_667566.1 110671009 4200114 1552034..1554607 1 NC_008245.1 This protein performs the mismatch recognition step during the DNA repair process; DNA mismatch repair protein MutS 1554607 muts 4200114 muts Francisella tularensis subsp. tularensis FSC198 DNA mismatch repair protein MutS YP_667566.1 1552034 D 393115 CDS YP_667567.1 110671010 4200735 1554679..1555122 1 NC_008245.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 1555122 secB2 4200735 secB2 Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecB YP_667567.1 1554679 D 393115 CDS YP_667568.1 110671011 4199880 1556563..1557780 1 NC_008245.1 Similar to AAP17334 (TRYP_ECOLI) Tyrosine-specific transport protein from E. coli (403 aa). FASTA: opt: 741 Z-score: 839.5 E(): 6.5e-39 Smith-Waterman score: 741; 32.663identity in 398 aa overlap ORF ftt1502; aromatic amino acid HAAP transporter 1557780 4199880 FTF1502 Francisella tularensis subsp. tularensis FSC198 aromatic amino acid HAAP transporter YP_667568.1 1556563 D 393115 CDS YP_667569.1 110671012 4200693 1557790..1558668 1 NC_008245.1 Similar to Q88B11 Integrase/recombinase XerC from Pseudomonas syringae (295 aa). FASTA: opt: 790 Z-score: 939.6 E(): 1.7e-44 Smith-Waterman score: 790; 42.177 identity in 294 aa overlap.; integrase/recombinase XerC 1558668 xerC 4200693 xerC Francisella tularensis subsp. tularensis FSC198 integrase/recombinase XerC YP_667569.1 1557790 D 393115 CDS YP_667570.1 110671013 4199881 1558668..1559393 1 NC_008245.1 Similar to YHIQ_NEIGO P72077 Hypothetical 27.3kDa protein from Neisseria gonorrhoeae (254 aa). FASTA: opt: 350 Z-score: 423.8 E(): 9.3e-16 Smith-Waterman score: 350; 36.073 identity in 219 aa overlap. ORF ftt1504; hypothetical protein 1559393 4199881 FTF1504 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667570.1 1558668 D 393115 CDS YP_667571.1 110671014 4199882 complement(1559400..1560014) 1 NC_008245.1 ORF ftt1505c; hypothetical protein 1560014 4199882 FTF1505c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667571.1 1559400 R 393115 CDS YP_667572.1 110671015 4199883 1560102..1560680 1 NC_008245.1 ORF ftt1506; hypothetical protein 1560680 4199883 FTF1506 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667572.1 1560102 D 393115 CDS YP_667573.1 110671016 4199884 1560757..1561389 1 NC_008245.1 ORF ftt1507; hypothetical protein 1561389 4199884 FTF1507 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667573.1 1560757 D 393115 CDS YP_667574.1 110671017 4200609 complement(1561505..1563448) 1 NC_008245.1 Similar to Q8EBQ4 GTP pyrophosphokinase from Shewenella oneidensis (735 aa). FASTA: opt: 1566 Z-score: 1723.7 E(): 3.7e-88 Smith-Waterman score: 1639; 39.744 identity in 624 aa overlap; GTP pyrophosphokinase 1563448 relA 4200609 relA Francisella tularensis subsp. tularensis FSC198 GTP pyrophosphokinase YP_667574.1 1561505 R 393115 CDS YP_667575.1 110671018 4199885 complement(1563817..1564173) 1 NC_008245.1 ORF ftt1509c; hypothetical protein 1564173 4199885 FTF1509c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667575.1 1563817 R 393115 CDS YP_667576.1 110671019 4199886 complement(1564207..1565403) 1 NC_008245.1 Similar to Q8DAB5 Tyrosine-specific transport protein from Vibrio vulnificus (401 aa). FASTA: opt: 804 Z-score: 930.6 E(): 5.5e-44 Smith-Waterman score: 804; 35.309 identity in 405 aa overlap ORF ftt1510; aromatic amino acid HAAP transporter 1565403 4199886 FTF1510c Francisella tularensis subsp. tularensis FSC198 aromatic amino acid HAAP transporter YP_667576.1 1564207 R 393115 CDS YP_667577.1 110671020 4199887 1565594..1566478 1 NC_008245.1 Similar to AAO90839 Membrane protein, putative from Coxiella burnetii (305 aa). FASTA: opt: 693 Z-score: 824.9 E(): 4.3e-38 Smith-Waterman score: 693; 38.776 identity in 294 aa overlap. ORF ftt1511; hypothetical protein 1566478 4199887 FTF1511 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667577.1 1565594 D 393115 CDS YP_667578.1 110671021 4199355 complement(1566468..1567394) 1 NC_008245.1 Similar to CAD85066 (Q82VD9) DnaJ N-terminal domain:DnaJ C terminal domain from Nitrosomonas europaea (314 aa). FASTA opt: 800 Z-score: 823.9 E(): 4.8e-38 39.482 identity in 309 aa overlap.; heat shock protein, hsp40 1567394 dnaJ2 4199355 dnaJ2 Francisella tularensis subsp. tularensis FSC198 heat shock protein, hsp40 YP_667578.1 1566468 R 393115 CDS YP_667579.1 110671022 4199889 complement(1568324..1568695) 1 NC_008245.1 ORF ftt1514c; hypothetical protein 1568695 4199889 FTF1514c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667579.1 1568324 R 393115 CDS YP_667580.1 110671023 4199890 complement(1568703..1569302) 1 NC_008245.1 Similar to Q8XMV7 Hypothetical protein CPE0581 from Clostridium perfrigens (195 aa). FASTA: opt: 348 Z-score: 432.5 E(): 3.1e-16 Smith-Waterman score: 348; 33.333 identity in 189 aa overlap. FTF1563 is more likely to be phosphatidylcoline synthase. ORF ftt1515c; hypothetical protein 1569302 4199890 FTF1515c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667580.1 1568703 R 393115 CDS YP_667581.1 110671024 4199891 complement(1570720..1571439) 1 NC_008245.1 Similar to Q92KR0 hypothetical transmembrane protein from Rhizobium meliloti (266aa). FASTA: opt: 566 Z-score: 664.8 E(): 3.5e-29 Smith-Waterman score: 566; 40.090 identity in 222 aa overlap. ORF ftt1517c; hypothetical protein 1571439 4199891 FTF1517c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667581.1 1570720 R 393115 CDS YP_667582.1 110671025 4200760 1571469..1572041 1 NC_008245.1 Similar to Q8A7W3 methylated-DNA--protein-cysteine methyltransferase from Bacteroides thetaiotaomicron (181aa). FASTA: opt: 443 Z-score: 536.8 bits: 106.7 E(): 4.8e-22 Smith-Waterman score: 443; 44.375 identity in 160 aa overlap. ogt in E.coli is a bifunctional protein containing an autotranscriptional activator at its carboxy domain and a methylated-DNA--protein-cysteine methyltransferase (MGMT) domain at its carboxy terminus. In Bacillus subtilis these two functions are independent and are referred to as adaA and adaB respectively; methylated-DNA--protein-cysteine methyltransferase 1572041 ogt 4200760 ogt Francisella tularensis subsp. tularensis FSC198 methylated-DNA--protein-cysteine methyltransferase YP_667582.1 1571469 D 393115 CDS YP_667583.1 110671026 4199206 complement(1572525..1573862) 1 NC_008245.1 Similar to Q8A4M6 glutamate/gamma-aminobutyrate anti-porter from Bacteroides thetaiotaomicron. FASTA: opt: 911 Z-score: 1001.3 E(): 6.4e-48 Smith-Waterman score: 911; 31.696identity in 448 aa overlap.; glutamate/gamma-aminobutyrate anti-porter 1573862 gabP 4199206 gabP Francisella tularensis subsp. tularensis FSC198 glutamate/gamma-aminobutyrate anti-porter YP_667583.1 1572525 R 393115 CDS YP_667584.1 110671027 4199893 complement(1575113..1575703) 1 NC_008245.1 Similar to Q8P4A2, hypothetical protein XCC3808 from Xanthamonas campestris (258aa). FASTA: opt:421 Z-score: 521.1 E(): 3.6e-21 Smith-Waterman score: 421; 34.536 identity in 194 aa overlap ORF ftt1522c; hypothetical protein 1575703 4199893 FTF1522c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667584.1 1575113 R 393115 CDS YP_667585.1 110671028 4199297 complement(1576072..1576815) 1 NC_008245.1 ISFtu2. Transposase, member of the IS5 family. Identical to Q8GM03 putative transposase from Francisella tularensis (247 aa).; transposase 1576815 isftu2 4199297 isftu2 Francisella tularensis subsp. tularensis FSC198 transposase YP_667585.1 1576072 R 393115 CDS YP_667586.1 110671029 4200201 complement(1577297..1581565) 1 NC_008245.1 Similar to Q8ZE61 ATP-dependent helicase from Yersinia pestis (1280 aa). FASTA: opt: 2018 Z-score: 2071.8 E(): 1.5e-107 Smith-Waterman score: 2974; 38.714 identity in 1400 aa overlap; ATp-dependent helicase 1581565 hrpA 4200201 hrpA Francisella tularensis subsp. tularensis FSC198 ATp-dependent helicase YP_667586.1 1577297 R 393115 CDS YP_667587.1 110671030 4200118 complement(1581589..1582476) 1 NC_008245.1 ORF ftt1525c; hypothetical protein 1582476 4200118 FTF1525c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667587.1 1581589 R 393115 CDS YP_667588.1 110671031 4200652 complement(1582490..1584733) 1 NC_008245.1 Similar to Q8EDW2 Isocitrate dehydrogenase,NADP-dependent from Shewanella oneidensis (741 aa). FASTA: opt: 3146 Z-score: 3332.9 E(): 8.6e-178 65.574identity in 732 aa overlap; isocitrate dehydrogenase 1584733 idh 4200652 idh Francisella tularensis subsp. tularensis FSC198 isocitrate dehydrogenase YP_667588.1 1582490 R 393115 CDS YP_667589.1 110671032 4199687 complement(1584737..1585102) 1 NC_008245.1 Similar to Q87MH6 Arsenate reductase from Vibrio parahaemolyticus (116 aa). FASTA: opt: 287 Z-score: 374.9 E(): 5e-13 Smith-Waterman score: 287; 38.739 identity in 111 aa overlap; arsenate reductase 1585102 arsC2 4199687 arsC2 Francisella tularensis subsp. tularensis FSC198 arsenate reductase YP_667589.1 1584737 R 393115 CDS YP_667590.1 110671033 4199403 1585318..1587006 1 NC_008245.1 Similar to Q8EE09 Long-chain-fatty-acid--CoA ligase from Shewanella oneidensis (557 aa). FASTA: opt: 1556 Z-score: 1747.3 E(): 1.8e-89 Smith-Waterman score: 1556; 42.908identity in 557 aa overlap; long chain fatty acid CoA ligase 1587006 fadD2 4199403 fadD2 Francisella tularensis subsp. tularensis FSC198 long chain fatty acid CoA ligase YP_667590.1 1585318 D 393115 CDS YP_667591.2 162139940 4199404 1587044..1589284 1 NC_008245.1 functions in fatty acid oxidation; converts acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; acyl-CoA dehydrogenase 1589284 fadE 4199404 fadE Francisella tularensis subsp. tularensis FSC198 acyl-CoA dehydrogenase YP_667591.2 1587044 D 393115 CDS YP_667592.1 110671035 4199401 1589357..1592053 1 NC_008245.1 N-terminal (85 aa) similar to CBP_YEAST (P31787) Acyl-CoA-binding protein (ACBP) from Saccharomyces cerevisiae (86 aa). FASTA opt: 206 Z-score: 281.8 E(): 7.6e-08 Smith-Waterman score: 206; 44.444identity in 72 aa overlap. C-terminus (110-898) similar to Q8P986 3-hydroxacyl-CoA dehydrogenase from Xanthomonas campestris (790 aa). FASTA: opt: 2495 Z-score: 2747.5 E(): 3.5e-145 Smith-Waterman score: 2495;48.228 identity in 790 aa overlap No match to first 110 aa with Q8P986 or any other 3-hydroxacyl-CoA dehydrogenase. Acyl-CoA-binding protein has only been described in eukaryotes unknown EC_number 5.2.2.3; bifunctional 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein 1592053 fadB/acbP 4199401 fadB/acbP Francisella tularensis subsp. tularensis FSC198 bifunctional 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein YP_667592.1 1589357 D 393115 CDS YP_667593.1 110671036 4199684 1592066..1593253 1 NC_008245.1 Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation; acetyl-CoA acetyltransferase 1593253 fadA 4199684 fadA Francisella tularensis subsp. tularensis FSC198 acetyl-CoA acetyltransferase YP_667593.1 1592066 D 393115 CDS YP_667594.1 110671037 4200119 1593315..1593803 1 NC_008245.1 Similar to Q8ABB1 Conserved hypothetical protein from Bacteriodes thetaiotaomicron (163 aa). FASTA: opt: 237 Z-score: 310.7 E(): 1.9e-09 Smith-Waterman score: 237; 27.097 identity in 155 aa overlap ORF ftt1532; hypothetical protein 1593803 4200119 FTF1532 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667594.1 1593315 D 393115 CDS YP_667595.1 110671038 4200121 complement(1595463..1596878) 1 NC_008245.1 Similar to Q8P596 Pre-B cell enhancing factor related protein from Xanthomonas campestris (468 aa). FASTA: opt: 1453 Z-score: 1660.8 E(): 1.2e-84 Smith-Waterman score: 1453; 49.260 identity in 473 aa overlap ORF ftt1534c; putative nicotinate phosphoribosyltransferase 1596878 4200121 FTF1534c Francisella tularensis subsp. tularensis FSC198 putative nicotinate phosphoribosyltransferase YP_667595.1 1595463 R 393115 CDS YP_667596.1 110671039 4200123 complement(1598221..1599135) 1 NC_008245.1 Similar to Q8RGP8 Hypothetical protein from Mycoplasma penetrans (310 aa). FASTA: opt: 805 Z-score: 956.1 E(): 2.1e-45 Smith-Waterman score: 805; 42.308 identity in 312 aa overlap ORF ftt1536c; hypothetical protein 1599135 4200123 FTF1536c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667596.1 1598221 R 393115 CDS YP_667597.1 110671040 4200124 complement(1599275..1600669) 1 NC_008245.1 Similar to Q8DAW7 Conserved hypothetical protein from Vibrio vulnificus (448 aa). FASTA: opt: 455 Z-score: 511.9 E(): 1.2e-20 Smith-Waterman score: 520; 26.539 identity in 471 aa overlap ORF ftt1537c; hypothetical protein 1600669 4200124 FTF1537c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667597.1 1599275 R 393115 CDS YP_667598.1 110671041 4200125 complement(1600675..1601658) 1 NC_008245.1 ORF ftt1538c; hypothetical protein 1601658 4200125 FTF1538c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667598.1 1600675 R 393115 CDS YP_667599.1 110671042 4200126 complement(1601669..1603099) 1 NC_008245.1 Similar to Q9PJ34 Putative periplasmic protein from Campylobacter jejuni (445 aa). FASTA: opt: 520 Z-score: 539.8 E(): 3.2e-22 Smith-Waterman score: 530; 27.673 identity in 477 aa overlap ORF ftt1539c; hypothetical protein 1603099 4200126 FTF1539c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667599.1 1601669 R 393115 CDS YP_667600.1 110671043 4200127 complement(1603156..1603764) 1 NC_008245.1 Similar to Q8EHZ4 Conserved hypothetical protein from Shewanella oneidensis (197 aa). FASTA: opt: 542 Z-score: 654.0 E(): 1.4e-28 Smith-Waterman score: 542; 41.584 identity in 202 aa overlap ORF ftt1540c; hypothetical protein 1603764 4200127 FTF1540c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667600.1 1603156 R 393115 CDS YP_667601.1 110671044 4200128 complement(1603967..1604341) 1 NC_008245.1 ORF ftt1541c; hypothetical protein 1604341 4200128 FTF1541c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667601.1 1603967 R 393115 CDS YP_667602.1 110671045 4200763 complement(1604459..1604995) 1 NC_008245.1 Similar to Q57483 Outer membrane protein 26 precursor (197 aa). FASTA: opt: 139 Z-score: 159.9 E(): 0.47 Smith-Waterman score: 139; 25.641 identity in 195 aa overlap; hypothetical protein 1604995 omp26 4200763 omp26 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667602.1 1604459 R 393115 CDS YP_667603.1 110671046 4200129 1605138..1605827 1 NC_008245.1 Similar to Q8UGQ7 Two component response regulator from Agrobacterium tumefaciens (252 aa). FASTA: opt: 684 Z-score: 819.7 E(): 8.4e-38 Smith-Waterman score: 684; 45.333 identity in 225 aa overlap ORF ftt1543; two component response regulator 1605827 4200129 FTF1543 Francisella tularensis subsp. tularensis FSC198 two component response regulator YP_667603.1 1605138 D 393115 CDS YP_667604.1 110671047 4200176 join(1609635..1609961,1609961..1610419) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1610419 isftu1 4200176 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667604.1 1609635 D 393115 CDS YP_667605.1 110671048 4200044 1611130..1611585 1 NC_008245.1 ORF ftt1549; hypothetical protein 1611585 4200044 FTF1549 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667605.1 1611130 D 393115 CDS YP_667606.1 110671049 4200045 1611679..1612221 1 NC_008245.1 Similar to Q8EBW9 Conserved hypothetical protein (183 aa). FASTA: opt: 505 Z-score: 618.4 E(): 1.4e-26 Smith-Waterman score: 505; 40.237identity in 169 aa overlap ORF ftt1550; hypothetical protein 1612221 4200045 FTF1550 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667606.1 1611679 D 393115 CDS YP_667607.1 110671050 4200046 1612223..1612966 1 NC_008245.1 Similar to Q888B1 Oxidoreductase,short chain dehydrogenase from Pseudomonas syringae (262 aa). FASTA: opt: 390 Z-score: 468.5 E(): 3e-18 Smith-Waterman score: 445; 32.143 identity in 252 aa overlap ORF ftt1551; oxidoreductase 1612966 4200046 FTF1551 Francisella tularensis subsp. tularensis FSC198 oxidoreductase YP_667607.1 1612223 D 393115 CDS YP_667608.1 110671051 4200761 1612980..1613807 1 NC_008245.1 Similar to Q8PMP8 Delta 9 acyl-lipid fatty acid desaturase from Xanthomonas axonopodis (298 aa). FASTA: opt: 851 Z-score: 1027.5 E(): 2.2e-49 Smith-Waterman score: 851; 45.149identity in 268 aa overlap; Delta 9 acyl-lipid fatty acid desaturase 1613807 ole1 4200761 ole1 Francisella tularensis subsp. tularensis FSC198 Delta 9 acyl-lipid fatty acid desaturase YP_667608.1 1612980 D 393115 CDS YP_667609.1 110671052 4200769 complement(1613831..1616128) 1 NC_008245.1 Similar to Q8EAG7 Ribonuclease R from (808 aa). FASTA: opt: 1840 Z-score: 1989.4 E(): 5.9e-103 Smith-Waterman score: 1863; 41.602 identity in 774 aa overlap; ribonuclease R 1616128 rnr 4200769 rnr Francisella tularensis subsp. tularensis FSC198 ribonuclease R YP_667609.1 1613831 R 393115 CDS YP_667610.1 110671053 4200725 complement(1616121..1617029) 1 NC_008245.1 catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs; tRNA pseudouridine synthase B 1617029 truB 4200725 truB Francisella tularensis subsp. tularensis FSC198 tRNA pseudouridine synthase B YP_667610.1 1616121 R 393115 CDS YP_667611.1 110671054 4199225 complement(1617016..1617708) 1 NC_008245.1 cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity; ribonuclease III 1617708 rnc 4199225 rnc Francisella tularensis subsp. tularensis FSC198 ribonuclease III YP_667611.1 1617016 R 393115 CDS YP_667612.1 110671055 4199512 complement(1617715..1618578) 1 NC_008245.1 Similar to AAO90612 9 83CL5) Signal peptidase I from Coxiella burnetii (259 aa). FASTA: opt: 716 Z-score: 839.6 E(): 6.5e-39 Smith-Waterman score: 717; 39.416 identity in 274 aa overlap; signal peptidase I 1618578 lepB 4199512 lepB Francisella tularensis subsp. tularensis FSC198 signal peptidase I YP_667612.1 1617715 R 393115 CDS YP_667613.1 110671056 4200047 complement(1618583..1619269) 1 NC_008245.1 Similar to AAO90736 DNA-binding response regulator from Coxiella burnetii (223 aa). FASTA: opt: 669 Z-score: 771.7 E(): 3.9e-35 Smith-Waterman score: 669; 45.778 identity in 225 aa overlap ORF ftt1557c; two-component response regulator 1619269 4200047 FTF1557c Francisella tularensis subsp. tularensis FSC198 two-component response regulator YP_667613.1 1618583 R 393115 CDS YP_667614.1 110671057 4199943 complement(1621046..1621870) 1 NC_008245.1 Similar to Q87LJ5 Pyrroline-5-carboxylate reductase from Vibrio parahaemolyticus (272 aa). FASTA: opt: 656 Z-score: 770.0 E(): 4.9e-35 Smith-Waterman score: 656; 39.925identity in 268 aa overlap; pyrroline-5-carboxylate reductase 1621870 proC 4199943 proC Francisella tularensis subsp. tularensis FSC198 pyrroline-5-carboxylate reductase YP_667614.1 1621046 R 393115 CDS YP_667615.1 110671058 4200049 1621977..1622555 1 NC_008245.1 HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine; putative deoxyribonucleotide triphosphate pyrophosphatase 1622555 4200049 FTF1560 Francisella tularensis subsp. tularensis FSC198 putative deoxyribonucleotide triphosphate pyrophosphatase YP_667615.1 1621977 D 393115 CDS YP_667616.1 110671059 4199509 1622660..1623955 1 NC_008245.1 Similar to Q87VF9 3-deoxy-D-manno-octulosonic-acid transferase from Pseudomonas syringae (447 aa). FASTA: opt: 1099 Z-score: 1251.3 E(): 7.6e-62 Smith-Waterman score: 1099; 40.143 identity in 421 aa overlap; 3-deoxy-D-manno-octulosonic-acid transferase 1623955 kdtA 4199509 kdtA Francisella tularensis subsp. tularensis FSC198 3-deoxy-D-manno-octulosonic-acid transferase YP_667616.1 1622660 D 393115 CDS YP_667617.1 110671060 4199298 1623952..1624833 1 NC_008245.1 Similar to P45204 Geranyltranstransferase from Haemophilus influenzae (295 aa). FASTA: opt: 728 Z-score: 859.4 E(): 5.1e-40 Smith-Waterman score: 728; 43.972 identity in 282 aa overlap; geranyltranstransferase 1624833 ispA 4199298 ispA Francisella tularensis subsp. tularensis FSC198 geranyltranstransferase YP_667617.1 1623952 D 393115 CDS YP_667618.1 110671061 4199229 1624881..1625645 1 NC_008245.1 Similar to O51265 Phosphatidyltransferase from Borrelia burgdorferi (234 aa). FASTA: opt: 438 Z-score: 531.7 E(): 9.1e-22 Smith-Waterman score: 496; 35.743 identity in 249 aa overlap; phosphatidylcholine synthase 1625645 pcs 4199229 pcs Francisella tularensis subsp. tularensis FSC198 phosphatidylcholine synthase YP_667618.1 1624881 D 393115 CDS YP_667619.1 110671062 4200050 1625715..1626524 1 NC_008245.1 Similar to Q9I6Z1 Hypothetical protein PA0141 from Pseudomonas aeruginosa (298 aa). FASTA: opt: 1210 Z-score: 1427.9 E(): 1.1e-71 Smith-Waterman score: 1210; 62.738 identity in 263 aa overlap ORF ftt1564; hypothetical protein 1626524 4200050 FTF1564 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667619.1 1625715 D 393115 CDS YP_667620.1 110671063 4200052 complement(1628345..1628641) 1 NC_008245.1 ORF ftt1566c; hypothetical protein 1628641 4200052 FTF1566c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667620.1 1628345 R 393115 CDS YP_667621.1 110671064 4200053 complement(1628812..1629399) 1 NC_008245.1 ORF ftt1567c; hypothetical protein 1629399 4200053 FTF1567c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667621.1 1628812 R 393115 CDS YP_667622.1 110671065 4199570 complement(1629458..1630600) 1 NC_008245.1 catalyzes the formation of lipid A disaccharide from UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is a precursor of lipid A that anchors LPS to the OM; lipid-A-disaccharide synthase 1630600 lpxB 4199570 lpxB Francisella tularensis subsp. tularensis FSC198 lipid-A-disaccharide synthase YP_667622.1 1629458 R 393115 CDS YP_667623.1 110671066 4199569 complement(1630600..1631379) 1 NC_008245.1 catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis; UDP-N-acetylglucosamine acyltransferase 1631379 lpxA 4199569 lpxA Francisella tularensis subsp. tularensis FSC198 UDP-N-acetylglucosamine acyltransferase YP_667623.1 1630600 R 393115 CDS YP_667624.1 110671067 4199683 complement(1631382..1631873) 1 NC_008245.1 in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP; (3R)-hydroxymyristoyl-ACP dehydratase 1631873 fabZ 4199683 fabZ Francisella tularensis subsp. tularensis FSC198 (3R)-hydroxymyristoyl-ACP dehydratase YP_667624.1 1631382 R 393115 CDS YP_667625.1 110671068 4199572 complement(1631888..1632901) 1 NC_008245.1 adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase 1632901 lpxD 4199572 lpxD Francisella tularensis subsp. tularensis FSC198 UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase YP_667625.1 1631888 R 393115 CDS YP_667626.1 110671069 4200080 complement(1632920..1633423) 1 NC_008245.1 Similar to OMPH_YEREN Cationic 19 kDa outer membrane protein from Yersinia enterocolitica (169 aa). FASTA: opt: 245 Z-score: 251.8 E(): 3.6e-06 Smith-Waterman score: 245; 28.000 identity in 150 aa overlap; outer membrane protein OmpH 1633423 ompH 4200080 ompH Francisella tularensis subsp. tularensis FSC198 outer membrane protein OmpH YP_667626.1 1632920 R 393115 CDS YP_667627.1 110671070 4200054 complement(1633559..1635937) 1 NC_008245.1 Similar to Q88MH2 Outer membrane protein,bacterial surface antigen family from Pseudomonas putida (786 aa). FASTA: opt: 1561 Z-score: 1675.2 E(): 1.9e-85 Smith-Waterman score: 1561; 32.959 identity in 801 aa overlap ORF ftt1573c; outer membrane protein 1635937 4200054 FTF1573c Francisella tularensis subsp. tularensis FSC198 outer membrane protein YP_667627.1 1633559 R 393115 CDS YP_667628.1 110671071 4199667 complement(1635943..1637100) 1 NC_008245.1 catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate; 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1637100 dxr 4199667 dxr Francisella tularensis subsp. tularensis FSC198 1-deoxy-D-xylulose 5-phosphate reductoisomerase YP_667628.1 1635943 R 393115 CDS YP_667629.1 110671072 4200367 complement(1637106..1639712) 1 NC_008245.1 Similar to GYRA_SALTY (P37411) DNA gyrase subunit A from Salmonella typhimurium (878 aa). FASTA: opt: 3442 Z-score: 3640.6 E(): 6.3e-195 Smith-Waterman score: 3442; 60.665 identity in 872 aa overlap; DNA gyrase subunit A 1639712 gyrA 4200367 gyrA Francisella tularensis subsp. tularensis FSC198 DNA gyrase subunit A YP_667629.1 1637106 R 393115 CDS YP_667630.1 110671073 4200055 1639971..1640516 1 NC_008245.1 ORF ftt1576; hypothetical protein 1640516 4200055 FTF1576 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667630.1 1639971 D 393115 CDS YP_667631.1 110671074 4200056 1640552..1641637 1 NC_008245.1 ORF ftt1577; hypothetical protein 1641637 4200056 FTF1577 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667631.1 1640552 D 393115 CDS YP_667632.1 110671075 4198983 complement(1641672..1642334) 1 NC_008245.1 Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine; uracil-DNA glycosylase 1642334 ung 4198983 ung Francisella tularensis subsp. tularensis FSC198 uracil-DNA glycosylase YP_667632.1 1641672 R 393115 CDS YP_667633.1 110671076 4200057 complement(1642331..1645204) 1 NC_008245.1 Similar to O25314 Type III restriction enzyme R protein from Helicobacter pylori (1001 aa). FASTA: opt: 1491 Z-score: 1485.7 E(): 6.6e-75 Smith-Waterman score: 2480; 42.828 identity in 990 aa overlap ORF ftt1579c; Type III restriction enzyme 1645204 4200057 FTF1579c Francisella tularensis subsp. tularensis FSC198 Type III restriction enzyme YP_667633.1 1642331 R 393115 CDS YP_667634.1 110671077 4200058 complement(1645441..1645971) 1 NC_008245.1 Identical to AAP83333 Hypothetical protein from Francisella tularensis ssp. tularensis strain SchuS4 (FSC237) (176 aa). ORF ftt1580c; hypothetical protein 1645971 4200058 FTF1580c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667634.1 1645441 R 393115 CDS YP_667635.1 110671078 4200059 complement(1646040..1646324) 1 NC_008245.1 Similar to Q8DE33 Predicted endonuclease from Vibrio vulnificus (94 aa). FASTA: opt: 387 Z-score: 525.2 E(): 2.1e-21 Smith-Waterman score: 387; 61.798identity in 89 aa overlap ORF ftt1581c; endonuclease 1646324 4200059 FTF1581c Francisella tularensis subsp. tularensis FSC198 endonuclease YP_667635.1 1646040 R 393115 CDS YP_667636.1 110671079 4200177 complement(join(1648680..1649138,1649138..1649464)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1649464 isftu1 4200177 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667636.1 1648680 R 393115 CDS YP_667637.1 110671080 4199705 1649546..1649710 1 NC_008245.1 ORF ftt1585; hypothetical protein 1649710 4199705 FTF1585 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667637.1 1649546 D 393115 CDS YP_667638.1 110671081 4199706 complement(1649724..1650005) 1 NC_008245.1 ORF ftt1586c; hypothetical protein 1650005 4199706 FTF1586c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667638.1 1649724 R 393115 CDS YP_667639.1 110671082 4199709 complement(1651886..1652629) 1 NC_008245.1 ORF ftt1589c; hypothetical protein 1652629 4199709 FTF1589c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667639.1 1651886 R 393115 CDS YP_667640.1 110671083 4198979 complement(1652632..1653327) 1 NC_008245.1 Involved in ubiquinone biosynthesis; 3-demethylubiquinone-9 3-methyltransferase 1653327 ubiG 4198979 ubiG Francisella tularensis subsp. tularensis FSC198 3-demethylubiquinone-9 3-methyltransferase YP_667640.1 1652632 R 393115 CDS YP_667641.1 110671084 4199710 1653430..1654569 1 NC_008245.1 Similar to Q9Z674 Lipoprotein from Zymomonas mobilis (256 aa). FASTA: opt: 352 Z-score: 404.8 E(): 1.1e-14 Smith-Waterman score: 352; 31.739 identity in 230 aa overlap ORF ftt1591; lipoprotein 1654569 4199710 FTF1591 Francisella tularensis subsp. tularensis FSC198 lipoprotein YP_667641.1 1653430 D 393115 CDS YP_667642.1 110671085 4199713 1658492..1659484 1 NC_008245.1 Similar to Q8D7C4 Transcriptional regulator from Vibrio vulnificus (301 aa). FASTA: opt: 364 Z-score: 428.7 E(): 5e-16 Smith-Waterman score: 364; 33.010 identity in 206 aa overlap ORF ftt1594; LysR family transcriptional regulator 1659484 4199713 FTF1594 Francisella tularensis subsp. tularensis FSC198 LysR family transcriptional regulator YP_667642.1 1658492 D 393115 CDS YP_667643.1 110671086 4199714 1659725..1659880 1 NC_008245.1 ORF ftt1595; hypothetical protein 1659880 4199714 FTF1595 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667643.1 1659725 D 393115 CDS YP_667644.1 110671087 4199715 1660053..1660451 1 NC_008245.1 ORF ftt1596; hypothetical protein 1660451 4199715 FTF1596 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667644.1 1660053 D 393115 CDS YP_667645.1 110671088 4199716 1660518..1661975 1 NC_008245.1 ORF ftt1597; hypothetical protein 1661975 4199716 FTF1597 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667645.1 1660518 D 393115 CDS YP_667646.1 110671089 4199717 1662039..1664873 1 NC_008245.1 This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein. ORF ftt1598; hypothetical protein 1664873 4199717 FTF1598 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667646.1 1662039 D 393115 CDS YP_667647.1 110671090 4200178 complement(join(1664970..1665428,1665428..1665754)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1665754 isftu1 4200178 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667647.1 1664970 R 393115 CDS YP_667648.1 110671091 4199200 complement(1666239..1667753) 1 NC_008245.1 Similar to Q87NJ5 Fumarate hydratase, class I from Vibrio parahaemolyticus (505 aa). FASTA: opt: 2398 Z-score: 2635.2 E(): 6.3e-139 Smith-Waterman score: 2398; 69.384 identity in 503 aa overlap; fumerate hydratase 1667753 fumA 4199200 fumA Francisella tularensis subsp. tularensis FSC198 fumerate hydratase YP_667648.1 1666239 R 393115 CDS YP_667649.1 110671092 4200604 complement(1668508..1670547) 1 NC_008245.1 Similar to Q88C73 ATP-dependent DNA helicase RecG from Pseudomonas putida (692 aa). FASTA: opt: 1698 Z-score: 1867.5 E(): 3.6e-96 Smith-Waterman score: 1698; 41.314 identity in 685 aa overlap; ATP-dependent DNA helicase RecG 1670547 recG 4200604 recG Francisella tularensis subsp. tularensis FSC198 ATP-dependent DNA helicase RecG YP_667649.1 1668508 R 393115 CDS YP_667650.1 110671093 4199718 1670677..1671021 1 NC_008245.1 ORF ftt1602; hypothetical protein 1671021 4199718 FTF1602 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667650.1 1670677 D 393115 CDS YP_667651.1 110671094 4199003 1671155..1671391 1 NC_008245.1 required for 70S ribosome assembly; 50S ribosomal protein L28 1671391 rpmB 4199003 rpmB Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L28 YP_667651.1 1671155 D 393115 CDS YP_667652.1 110671095 4199007 1671418..1671573 1 NC_008245.1 in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif; 50S ribosomal protein L33 1671573 rpmG 4199007 rpmG Francisella tularensis subsp. tularensis FSC198 50S ribosomal protein L33 YP_667652.1 1671418 D 393115 CDS YP_667653.1 110671096 4199846 1671608..1672294 1 NC_008245.1 Similar to Q87RC3 Septum site-determining protein MinC from Vibrio parahaemolyticus (220 aa). FASTA: opt: 444 Z-score: 518.9 E(): 4.7e-21 Smith-Waterman score: 444; 39.196 identity in 199 aa overlap; septum site-determining protein MinC 1672294 minC 4199846 minC Francisella tularensis subsp. tularensis FSC198 septum site-determining protein MinC YP_667653.1 1671608 D 393115 CDS YP_667654.1 110671097 4199847 1672334..1673158 1 NC_008245.1 Similar to Q8FWX1 Septum site-determining protein MinD from Brucella suis(271 aa). FASTA: opt: 1091 Z-score: 1181.8 E(): 5.6e-58 Smith-Waterman score: 1091; 62.825 identity in 269 aa overlap; septum site-determining protein MinD 1673158 minD 4199847 minD Francisella tularensis subsp. tularensis FSC198 septum site-determining protein MinD YP_667654.1 1672334 D 393115 CDS YP_667655.1 110671098 4199848 1673161..1673433 1 NC_008245.1 works in conjunction with MinC and MinD to enable cell division at the midpoint of the long axis of the cell; cell division topological specificity factor MinE 1673433 minE 4199848 minE Francisella tularensis subsp. tularensis FSC198 cell division topological specificity factor MinE YP_667655.1 1673161 D 393115 CDS YP_667656.1 110671099 4199719 1673447..1674250 1 NC_008245.1 Similar to YRBF_HAEIN Probable ABC transporter ATP-binding protein HI1087 from Haemophilus influenzae (264 aa). FASTA: opt: 861 Z-score: 970.8 E(): 3.2e-46 Smith-Waterman score: 861; 50.958identity in 261 aa overlap Equivalent to yrbF of yrb operon in E.coli ORF ftt1608; ABC transporter ATP-binding protein 1674250 4199719 FTF1608 Francisella tularensis subsp. tularensis FSC198 ABC transporter ATP-binding protein YP_667656.1 1673447 D 393115 CDS YP_667657.1 110671100 4199720 1674254..1675018 1 NC_008245.1 Similar Q8VUQ9 Toluene resistance protein Trg1 from Pseudomonas putida (265 aa). FASTA: opt: 793 Z-score: 934.5 E(): 3.4e-44 Smith-Waterman score: 793; 55.869 identity in 213 aa overlap Equivalent to yrbE of yrb operon in E.coli ORF ftt1609; ABC transporter membrane protein 1675018 4199720 FTF1609 Francisella tularensis subsp. tularensis FSC198 ABC transporter membrane protein YP_667657.1 1674254 D 393115 CDS YP_667658.1 110671101 4199721 1675018..1675551 1 NC_008245.1 Similar to YRBD_HAEIN P45029 Hypothetical protein HI1085 from Haemophilus influenzae (167 aa). FASTA: opt: 256 Z-score: 320.6 E(): 5.3e-10 Smith-Waterman score: 281; 37.640identity in 178 aa overlap. Equivalent to yrbD of yrb operon in E.coli ORF ftt1610; hypothetical protein 1675551 4199721 FTF1610 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667658.1 1675018 D 393115 CDS YP_667659.1 110671102 4199722 1675619..1676287 1 NC_008245.1 Similar to YRBC_ECOLI Protein yrbC precursor from E. coli (211 aa). FASTA: opt: 230 Z-score: 278.3 E(): 1.2e-07 Smith-Waterman score: 230; 26.147 identity in 218 aa overlap Equivalent to yrbC of yrb operon in E.coli ORF ftt1611; hypothetical protein 1676287 4199722 FTF1611 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667659.1 1675619 D 393115 CDS YP_667660.1 110671103 4199723 1676287..1676580 1 NC_008245.1 Similar to YRBB_ECOLI Hypothetical protein yrbB from E. coli (97 aa). FASTA: opt: 116 Z-score: 165.8 E(): 0.22 Smith-Waterman score: 116; 31.915identity in 47 aa overlap Equivalent to yrbB of yrb operon in E.coli ORF ftt1612; hypothetical protein 1676580 4199723 FTF1612 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667660.1 1676287 D 393115 CDS YP_667661.1 110671104 4199247 1676597..1676833 1 NC_008245.1 Similar to Q8ZB55 BolA-like protein from Yersinia pestis (87 aa). FASTA: opt: 163 Z-score: 236.9 E(): 2.4e-05 Smith-Waterman score: 163; 35.526 identity in 76 aa overlap Equivalent to yrbA of yrb operon in E.coli ORF ftt1613; BolA-like protein 1676833 4199247 FTF1613 Francisella tularensis subsp. tularensis FSC198 BolA-like protein YP_667661.1 1676597 D 393115 CDS YP_667662.1 110671105 4199248 complement(1677113..1677796) 1 NC_008245.1 ORF ftt1614c; hypothetical protein 1677796 4199248 FTF1614c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667662.1 1677113 R 393115 CDS YP_667663.1 110671106 4200179 join(1678030..1678356,1678356..1678814) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1678814 isftu1 4200179 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667663.1 1678030 D 393115 CDS YP_667664.1 110671107 4199523 1679286..1680680 1 NC_008245.1 catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA; cysteinyl-tRNA synthetase 1680680 cysS 4199523 cysS Francisella tularensis subsp. tularensis FSC198 cysteinyl-tRNA synthetase YP_667664.1 1679286 D 393115 CDS YP_667665.1 110671108 4200377 1680684..1681628 1 NC_008245.1 involved in methylation of ribosomal protein L3; N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 1681628 hemK 4200377 hemK Francisella tularensis subsp. tularensis FSC198 N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase YP_667665.1 1680684 D 393115 CDS YP_667666.1 110671109 4200180 join(1683279..1683605,1683605..1684063) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1684063 isftu1 4200180 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667666.1 1683279 D 393115 CDS YP_667667.1 110671110 4199251 complement(1684472..1685323) 1 NC_008245.1 ORF ftt1621c; hypothetical protein 1685323 4199251 FTF1621c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667667.1 1684472 R 393115 CDS YP_667668.1 110671111 4199252 complement(1685336..1686256) 1 NC_008245.1 ORF ftt1622c; hypothetical protein 1686256 4199252 FTF1622c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667668.1 1685336 R 393115 CDS YP_667669.1 110671112 4199253 complement(1686275..1686595) 1 NC_008245.1 ORF ftt1623c; hypothetical protein 1686595 4199253 FTF1623c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667669.1 1686275 R 393115 CDS YP_667670.1 110671113 4199254 complement(1686667..1686987) 1 NC_008245.1 ORF ftt1624c; hypothetical protein 1686987 4199254 FTF1624c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667670.1 1686667 R 393115 CDS YP_667671.1 110671114 4199255 complement(1687087..1687677) 1 NC_008245.1 ORF ftt1625c; hypothetical protein 1687677 4199255 FTF1625c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667671.1 1687087 R 393115 CDS YP_667672.1 110671115 4199256 complement(1687688..1689094) 1 NC_008245.1 ORF ftt1626c; hypothetical protein 1689094 4199256 FTF1626c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667672.1 1687688 R 393115 CDS YP_667673.1 110671116 4199257 complement(1689091..1689894) 1 NC_008245.1 ORF ftt1627c; hypothetical protein 1689894 4199257 FTF1627c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667673.1 1689091 R 393115 CDS YP_667674.1 110671117 4199258 complement(1690295..1691605) 1 NC_008245.1 Similar to Q88C61 putative metal ion transporter from Pseudomonas putida (446 aa). FASTA: opt: 1033 Z-score: 1180.6 E(): 6.5e-58 Smith-Waterman score: 1035; 40.455 identity in 440 aa overlap ORF ftt1628c; metal ion transporter 1691605 4199258 FTF1628c Francisella tularensis subsp. tularensis FSC198 metal ion transporter YP_667674.1 1690295 R 393115 CDS YP_667675.1 110671118 4199259 complement(1691616..1693295) 1 NC_008245.1 ORF ftt1629c; hypothetical protein 1693295 4199259 FTF1629c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667675.1 1691616 R 393115 CDS YP_667676.1 110671119 4199127 complement(1693366..1694832) 1 NC_008245.1 Similar to PUTP_ECOLI Sodium/proline symporter from E. coli (501 aa). FASTA: opt: 1536 Z-score: 1735.2 E(): 8.4e-89 Smith-Waterman score: 1536; 46.531identity in 490 aa overlap; Sodium/proline permease 1694832 putP 4199127 putP Francisella tularensis subsp. tularensis FSC198 Sodium/proline permease YP_667676.1 1693366 R 393115 CDS YP_667677.1 110671120 4200294 complement(1695138..1696124) 1 NC_008245.1 type II fructose 1,6-bisphosphatae; in Escherichia coli this protein forms a dimer and binds manganese; fructose 1,6-bisphosphatase II 1696124 glpX 4200294 glpX Francisella tularensis subsp. tularensis FSC198 fructose 1,6-bisphosphatase II YP_667677.1 1695138 R 393115 CDS YP_667678.1 110671121 4199260 complement(1696144..1696782) 1 NC_008245.1 Similar to AAO91263 (Q83AV9) Hypothetical protein from Coxiella burnetti (205 aa). FASTA: opt: 552 Z-score: 685.2 E(): 2.6e-30 Smith-Waterman score: 552; 41.000 identity in 200 aa overlap ORF ftt1632c; hypothetical protein 1696782 4199260 FTF1632c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667678.1 1696144 R 393115 CDS YP_667679.1 110671122 4199261 complement(1696791..1698245) 1 NC_008245.1 Similiar to AAP09999 (Q81BU4) Lysine-specific permease from Bacillus cereus (491 aa). FASTA: opt: 1734 Z-score: 1972.4 bits: 374.4 E(): 5.2e-102 Smith-Waterman score: 1817; 53.459 identity in 477 aa overlap ORF ftt1633c; amino acid transporter 1698245 4199261 FTF1633c Francisella tularensis subsp. tularensis FSC198 amino acid transporter YP_667679.1 1696791 R 393115 CDS YP_667680.1 110671123 4199262 complement(1698493..1699161) 1 NC_008245.1 ORF ftt1634c; hypothetical protein 1699161 4199262 FTF1634c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667680.1 1698493 R 393115 CDS YP_667681.1 110671124 4199195 1699283..1701784 1 NC_008245.1 Similar to Q9I0M3 Cell division protein FtsK from Rickettsia prowazekii (811 aa). FASTA: opt: 2030 Z-score: 2059.0 E(): 7.7e-107 Smith-Waterman score: 2197; 45.808 identity in 823 aa overlap; cell division protein 1701784 ftsK 4199195 ftsK Francisella tularensis subsp. tularensis FSC198 cell division protein YP_667681.1 1699283 D 393115 CDS YP_667682.1 110671125 4199558 1701785..1702402 1 NC_008245.1 Similar to LOLA_PSEAE (Q9I0M4) Outer-membrane lipoproteins carrier from Pseudomonas aeruginosa (208 aa). FASTA: opt: 384 Z-score: 440.9 E(): 1e-16 Smith-Waterman score: 384; 30.476 identity in 210 aa overlap; lipoprotein releasing system subunit A, outer membrane lipoproteins carrier 1702402 lolA 4199558 lolA Francisella tularensis subsp. tularensis FSC198 lipoprotein releasing system subunit A, outer membrane lipoproteins carrier YP_667682.1 1701785 D 393115 CDS YP_667683.1 110671126 4199263 complement(1702800..1703003) 1 NC_008245.1 ORF ftt1637c; hypothetical protein 1703003 4199263 FTF1637c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667683.1 1702800 R 393115 CDS YP_667684.1 110671127 4199862 1703115..1704569 1 NC_008245.1 Similar to Q9KNI3 Potassium uptake protein TrkH from Vibrio cholerae (485 aa). FASTA: opt: 1470 Z-score: 1682.5 E(): 7.3e-86 Smith-Waterman score: 1470; 43.827 identity in 486 aa overlap; potassium uptake protein 1704569 trkH 4199862 trkH Francisella tularensis subsp. tularensis FSC198 potassium uptake protein YP_667684.1 1703115 D 393115 CDS YP_667685.1 110671128 4199264 complement(1704584..1705129) 1 NC_008245.1 ORF ftt1639c; hypothetical protein 1705129 4199264 FTF1639c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667685.1 1704584 R 393115 CDS YP_667686.1 110671129 4199820 1708673..1710097 1 NC_008245.1 Similar to O86076 Sucrose hydrolase from E. coli (477 aa). FASTA: opt: 787 Z-score: 912.8 E(): 5.4e-43 Smith-Waterman score: 787; 31.929 identity in 451 aa overlap ORF ftt1644; Beta-fructofuranosidase 1710097 4199820 FTF1644 Francisella tularensis subsp. tularensis FSC198 Beta-fructofuranosidase YP_667686.1 1708673 D 393115 CDS YP_667687.1 110671130 4199821 1710116..1711348 1 NC_008245.1 Similar to Q8EDM5 Conserved hypothetical protein from Shewanella oneidensis (384 aa). FASTA: opt: 372 Z-score: 419.7 E(): 1.6e-15 Smith-Waterman score: 372; 23.018 identity in 391 aa overlap ORF ftt1645; hypothetical protein 1711348 4199821 FTF1645 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667687.1 1710116 D 393115 CDS YP_667688.1 110671131 4199361 1711590..1711919 1 NC_008245.1 hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); possible defense mechanism against a harmful effect of D-tyrosine; D-tyrosyl-tRNA(Tyr) deacylase 1711919 dtd 4199361 dtd Francisella tularensis subsp. tularensis FSC198 D-tyrosyl-tRNA(Tyr) deacylase YP_667688.1 1711590 D 393115 CDS YP_667689.1 110671132 4199131 complement(1712290..1713036) 1 NC_008245.1 Similar to Q9WYG8 Dihydroorotate dehydrogenase from Pyrobaculum aerophilum (297 aa). FASTA: opt: 148 Z-score: 178.6 E(): 0.043 Smith-Waterman score: 191; 26.568 identity in 271 aa overlap; diyroorotate dehydrogenase 1713036 pyrD 4199131 pyrD Francisella tularensis subsp. tularensis FSC198 diyroorotate dehydrogenase YP_667689.1 1712290 R 393115 CDS YP_667690.1 110671133 4199133 complement(1713038..1713682) 1 NC_008245.1 Similar to PYRF_STAAM (Q9LCT0) Orotidine 5'-phosphate decarboxylase from Bradyrhizobium japonicum (237 aa). FASTA: opt: 228 z-score: 280.6 E(): 8.8e-08 Smith-Waterman score: 243; 28.061identity in 196 aa overlap; orotidine 5'-phosphate decarboxylase 1713682 pyrF 4199133 pyrF Francisella tularensis subsp. tularensis FSC198 orotidine 5'-phosphate decarboxylase YP_667690.1 1713038 R 393115 CDS YP_667691.1 110671134 4199823 complement(1717374..1717922) 1 NC_008245.1 Similar to Q93LJ4 Periplasmic monofunctional chorismate mutase from Salmonella typhimurium (181 aa). FASTA: opt: 186 Z-score: 223.0 E(): 0.00014 Smith-Waterman score: 186; 31.746 identity in 126 aa overlap ORF ftt1650; chorismate mutase 1717922 4199823 FTF1650c Francisella tularensis subsp. tularensis FSC198 chorismate mutase YP_667691.1 1717374 R 393115 CDS YP_667692.1 110671135 4199824 1718074..1718700 1 NC_008245.1 Similar to Q8KDD5 Hypothetical protein from Chlorobium tepidum (215 aa). FASTA: opt: 569 z-score: 706.8 E(): 1.6e-31 Smith-Waterman score: 569; 40.580 identity in 207 aa overlap ORF ftt1651; hypothetical protein 1718700 4199824 FTF1651 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667692.1 1718074 D 393115 CDS YP_667693.1 110671136 4199825 complement(1718701..1719570) 1 NC_008245.1 Similar to Q8EFC8 Transcriptional regulator, LysR family from Shewanella oneidensis (291 aa). FASTA: opt: 443 Z-score: 527.9 E(): 1.5e-21 Smith-Waterman score: 443; 29.831 identity in 295 aa overlap ORF ftt1652c; LysR family transcriptional regulator 1719570 4199825 FTF1652c Francisella tularensis subsp. tularensis FSC198 LysR family transcriptional regulator YP_667693.1 1718701 R 393115 CDS YP_667694.1 110671137 4199826 1719696..1720103 1 NC_008245.1 ORF ftt1653; hypothetical protein 1720103 4199826 FTF1653 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667694.1 1719696 D 393115 CDS YP_667695.1 110671138 4199673 1720192..1721157 1 NC_008245.1 Similar to AAO90332 (Q83DE0) Multidrug resistance protein from Coxiella burnetii (331 aa). FASTA: opt: 406 z-score: 447.9 E(): 4.2e-17 Smith-Waterman score: 406; 28.135 identity in 327 aa overlap; HlyD family secretion protein 1721157 emrA2 4199673 emrA2 Francisella tularensis subsp. tularensis FSC198 HlyD family secretion protein YP_667695.1 1720192 D 393115 CDS YP_667696.1 110671139 4199827 1721132..1722196 1 NC_008245.1 ORF ftt1655; hypothetical protein 1722196 4199827 FTF1655 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667696.1 1721132 D 393115 CDS YP_667697.1 110671140 4199828 complement(1722312..1722863) 1 NC_008245.1 Similiar to Q8PGE2 Hypothetical protein from Xanthomonas axonopodis(186 aa). FASTA: opt: 578 Z-score: 703.0 E(): 2.6e-31 Smith-Waterman score: 578; 51.462 identity in 171 aa overlap ORF ftt1656c; hypothetical protein 1722863 4199828 FTF1656c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667697.1 1722312 R 393115 CDS YP_667698.1 110671141 4199829 complement(1723327..1723530) 1 NC_008245.1 ORF ftt1657c; hypothetical protein 1723530 4199829 FTF1657c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667698.1 1723327 R 393115 CDS YP_667699.1 110671142 4199830 complement(1723577..1723831) 1 NC_008245.1 Similar to BAC68773 (Q82P70) Hypothetical protein from Streptomyces avermitilis (95 aa). FASTA: opt: 135 Z-score: 188.6 E(): 0.012 Smith-Waterman score: 135; 25.316 identity in 79 aa overlap ORF ftt1658c; hypothetical protein 1723831 4199830 FTF1658c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667699.1 1723577 R 393115 CDS YP_667700.1 110671143 4199831 1724084..1725109 1 NC_008245.1 ORF ftt1659; hypothetical protein 1725109 4199831 FTF1659 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667700.1 1724084 D 393115 CDS YP_667701.1 110671144 4199130 complement(1725137..1726483) 1 NC_008245.1 Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides; dihydroorotase 1726483 pyrC 4199130 pyrC Francisella tularensis subsp. tularensis FSC198 dihydroorotase YP_667701.1 1725137 R 393115 CDS YP_667702.1 110671145 4199147 1726615..1727295 1 NC_008245.1 catalyzes the S-adenosylmethionine-dependent transmethylation of thiopurine compounds; may be involved in selenium cycling by forming dimethylselenide and/or dimethyldiselenide; thiopurine S-methyltransferase 1727295 tmpT 4199147 tmpT Francisella tularensis subsp. tularensis FSC198 thiopurine S-methyltransferase YP_667702.1 1726615 D 393115 CDS YP_667703.1 110671146 4200315 1728889..1730055 1 NC_008245.1 catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers; carbamoyl phosphate synthase small subunit 1730055 carA 4200315 carA Francisella tularensis subsp. tularensis FSC198 carbamoyl phosphate synthase small subunit YP_667703.1 1728889 D 393115 CDS YP_667704.1 110671147 4200316 1730060..1733344 1 NC_008245.1 four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity; carbamoyl phosphate synthase large subunit 1733344 carB 4200316 carB Francisella tularensis subsp. tularensis FSC198 carbamoyl phosphate synthase large subunit YP_667704.1 1730060 D 393115 CDS YP_667705.1 110671148 4199129 1733486..1734406 1 NC_008245.1 Similar to PYRB_METJA Aspartate carbamoyltransferase from Methanococcus jannaschii (306 aa). FASTA: opt: 943 Z-score: 1081.5 E(): 2.2e-52 Smith-Waterman score: 943; 49.338 identity in 302 aa overlap; aspartate carbamoyltransferase 1734406 pyrB 4199129 pyrB Francisella tularensis subsp. tularensis FSC198 aspartate carbamoyltransferase YP_667705.1 1733486 D 393115 CDS YP_667706.1 110671149 4199833 complement(1734419..1735306) 1 NC_008245.1 Similar to AAP10961 (Q819E3) 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus (292 aa). FASTA: opt: 384 z-score: 452.2 E(): 2.4e-17 Smith-Waterman score: 384; 24.483 identity in 290 aa overlap ORF ftt1666c; 3-hydroxyisobutyrate dehydrogenase 1735306 4199833 FTF1666c Francisella tularensis subsp. tularensis FSC198 3-hydroxyisobutyrate dehydrogenase YP_667706.1 1734419 R 393115 CDS YP_667707.1 110671150 4199834 1735794..1736030 1 NC_008245.1 ORF ftt1667; hypothetical protein 1736030 4199834 FTF1667 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667707.1 1735794 D 393115 CDS YP_667708.1 110671151 4200728 1736357..1737580 1 NC_008245.1 Similar to Q8ZGH6 Serine transporter from Yersinia pestis (433 aa). FASTA: opt: 1336 Z-score: 1510.1 E(): 2.9e-76 Smith-Waterman score: 1336; 46.078identity in 408 aa overlap.; serine transporter 1737580 sdaC2 4200728 sdaC2 Francisella tularensis subsp. tularensis FSC198 serine transporter YP_667708.1 1736357 D 393115 CDS YP_667709.1 110671152 4200182 join(1738564..1738890,1738890..1739348) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1739348 isftu1 4200182 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667709.1 1738564 D 393115 CDS YP_667710.1 110671153 4199215 1740427..1741494 1 NC_008245.1 Similar to RIBD_AQUAE Riboflavin biosynthesis protein ribD from Aquiflex aeolicus (356 aa). FASTA: opt: 915 z-score: 1060.1 E(): 3.4e-51 Smith-Waterman score: 916; 43.824 identity in 340 aa overlap; riboflavin biosynthesis protein ribD 1741494 ribD 4199215 ribD Francisella tularensis subsp. tularensis FSC198 riboflavin biosynthesis protein ribD YP_667710.1 1740427 D 393115 CDS YP_667711.1 110671154 4199214 1741487..1742092 1 NC_008245.1 catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine; riboflavin synthase subunit alpha 1742092 ribB 4199214 ribB Francisella tularensis subsp. tularensis FSC198 riboflavin synthase subunit alpha YP_667711.1 1741487 D 393115 CDS YP_667712.1 110671155 4199213 1742085..1743296 1 NC_008245.1 Similar to GCH2_AQUAE Riboflavin biosynthesis protein ribA from Aquiflex aeolicus (406 aa). FASTA: opt: 1135 z-score: 1316.5 E(): 1.8e-65 Smith-Waterman score: 1135; 46.305 identity in 406 aa overlap; riboflavin biosynthesis protein ribA/GTP-cyclohydrolase II 1743296 ribA 4199213 ribA Francisella tularensis subsp. tularensis FSC198 riboflavin biosynthesis protein ribA/GTP-cyclohydrolase II YP_667712.1 1742085 D 393115 CDS YP_667713.1 110671156 4199217 1743306..1743749 1 NC_008245.1 Similar to AAO90192 (Q83DP8) Riboflavin synthase,beta subunit from Coxiella burnetii (151 aa). FASTA: opt: 496 z-score: 645.4 E(): 4.2e-28 Smith-Waterman score: 496; 53.571 identity in 140 aa overlap; riboflavin synthase beta subunit (6,7-dimethl-8-ribityllumazine synthase) 1743749 ribH 4199217 ribH Francisella tularensis subsp. tularensis FSC198 riboflavin synthase beta subunit (6,7-dimethl-8-ribityllumazine synthase) YP_667713.1 1743306 D 393115 CDS YP_667714.1 110671157 4199343 1743746..1744270 1 NC_008245.1 Similar to DEF_NEIMA (Q9JQN0) Peptide deformylase from Neisseria meningitidis (167 aa). FASTA: opt: 465 z-score: 571.7 E(): 5.4e-24 Smith-Waterman score: 465; 46.914 identity in 162 aa overlap; peptide deformylase 1744270 def2 4199343 def2 Francisella tularensis subsp. tularensis FSC198 peptide deformylase YP_667714.1 1743746 D 393115 CDS YP_667715.1 110671158 4199835 1744352..1745344 1 NC_008245.1 ORF ftt1676; hypothetical protein 1745344 4199835 FTF1676 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667715.1 1744352 D 393115 CDS YP_667716.1 110671159 4199865 complement(1745364..1746509) 1 NC_008245.1 catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; tRNA-specific 2-thiouridylase MnmA 1746509 mnmA 4199865 mnmA Francisella tularensis subsp. tularensis FSC198 tRNA-specific 2-thiouridylase MnmA YP_667716.1 1745364 R 393115 CDS YP_667717.1 110671160 4199511 complement(1746457..1748241) 1 NC_008245.1 binds to the ribosome on the universally-conserved alpha-sarcin loop; GTP-binding protein LepA 1748241 lepA 4199511 lepA Francisella tularensis subsp. tularensis FSC198 GTP-binding protein LepA YP_667717.1 1746457 R 393115 CDS YP_667718.1 110671161 4198958 1748511..1748783 1 NC_008245.1 binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase; 30S ribosomal protein S20 1748783 rpsT 4198958 rpsT Francisella tularensis subsp. tularensis FSC198 30S ribosomal protein S20 YP_667718.1 1748511 D 393115 CDS YP_667719.1 110671162 4200762 complement(1748857..1749213) 1 NC_008245.1 Similar to Q8KTI1 Outer membrane lipoprotein OmlA from Azaobacter vinelandii (171 aa). FASTA: opt: 208 Z-score: 273.7 E(): 2.1e-07 Smith-Waterman score: 208; 30.392 identity in 102 aa overlap; outer membrane lipoprotein 1749213 olmA 4200762 olmA Francisella tularensis subsp. tularensis FSC198 outer membrane lipoprotein YP_667719.1 1748857 R 393115 CDS YP_667720.1 110671163 4199563 complement(1749221..1749817) 1 NC_008245.1 Similar to Q8EK08 Phosphoheptose isomerase from Shewanella oneidensis (197 aa). FASTA: opt: 705 z-score: 847.8 E(): 2.3e-39 Smith-Waterman score: 705; 53.684 identity in 190 aa overlap; phosphoheptose isomerase 1749817 lpcA 4199563 lpcA Francisella tularensis subsp. tularensis FSC198 phosphoheptose isomerase YP_667720.1 1749221 R 393115 CDS YP_667721.1 110671164 4200153 complement(1752366..1753517) 1 NC_008245.1 Similar to Q8NM37 Permeases of the major facilitator superfamily from Corynebacterium glutamicum (398 aa). FASTA: opt: 440 z-score: 489.8 E(): 2e-19 Smith-Waterman score: 440; 24.211 identity in 380 aa overlap ORF ftt1683c; major facilitator transporter 1753517 4200153 FTF1683c Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667721.1 1752366 R 393115 CDS YP_667722.1 110671165 4200154 1753614..1754516 1 NC_008245.1 Similar to Q8D3N4 Transcriptional regulator from Vibrio vulnificus (307 aa). FASTA: opt: 510 Z-score: 590.3 E(): 5e-25 32.975 identity in 279 aa overlap ORF ftt1684; transcriptional regulator 1754516 4200154 FTF1684 Francisella tularensis subsp. tularensis FSC198 transcriptional regulator YP_667722.1 1753614 D 393115 CDS YP_667723.1 110671166 4200155 1754718..1755443 1 NC_008245.1 ORF ftt1685; hypothetical protein 1755443 4200155 FTF1685 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667723.1 1754718 D 393115 CDS YP_667724.1 110671167 4200156 complement(1755597..1756265) 1 NC_008245.1 Similar to Q894C5 Proline synthetase associated protein from Clostridium tetani (225 aa). FASTA: opt: 462 z-score: 549.8 E(): 8.9e-23 37.220 identity in 223 aa overlap ORF ftt1686c; hypothetical protein 1756265 4200156 FTF1686c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667724.1 1755597 R 393115 CDS YP_667725.1 110671168 4199781 complement(1756262..1756879) 1 NC_008245.1 glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA; 16S rRNA methyltransferase GidB 1756879 gidB 4199781 gidB Francisella tularensis subsp. tularensis FSC198 16S rRNA methyltransferase GidB YP_667725.1 1756262 R 393115 CDS YP_667726.1 110671169 4200157 1756979..1758184 1 NC_008245.1 Similar to Q8EFB8 Tyrosine-specific transport protein from Shewanella oneidensis (395 aa). FASTA: opt: 854 z-score: 967.0 E(): 5.2e-46 38.378 identity in 370 aa overlap ORF ftt1688; aromatic amino acid HAAP transporter 1758184 4200157 FTF1688 Francisella tularensis subsp. tularensis FSC198 aromatic amino acid HAAP transporter YP_667726.1 1756979 D 393115 CDS YP_667727.1 110671170 4200158 complement(1758181..1758873) 1 NC_008245.1 Similar to CAD84514 (Q82WQ9) Possible transmembrane protein from Nitrosomonas europaea (223 aa). FASTA: opt: 294 z-score: 358.6 E(): 4e-12 25.481 identity in 208 aa overlap ORF ftt1689c; hypothetical protein 1758873 4200158 FTF1689c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667727.1 1758181 R 393115 CDS YP_667728.1 110671171 4200159 1759277..1759942 1 NC_008245.1 Similar to Q9ABA7 NADH dehydrogenase from Caulobacter crescentus (220 aa). FASTA: opt: 429 z-score: 535.0 E(): 6e-22 35.545 identity in 211 aa overlap ORF ftt1690; NADH dehydrogenase 1759942 4200159 FTF1690 Francisella tularensis subsp. tularensis FSC198 NADH dehydrogenase YP_667728.1 1759277 D 393115 CDS YP_667729.1 110671172 4200160 1760213..1760737 1 NC_008245.1 This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein. ORF ftt1691; hypothetical protein 1760737 4200160 FTF1691 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667729.1 1760213 D 393115 CDS YP_667730.1 110671173 4200183 join(1760761..1761087,1761087..1761545) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1761545 isftu1 4200183 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667730.1 1760761 D 393115 CDS YP_667731.1 110671174 4200161 complement(1761688..1762452) 1 NC_008245.1 Similar to Q87SC0 conserved hypothetical protein from Vibrio parahaemolyticus (258 aa). FASTA: opt: 724 z-score: 845.1 E(): 3.2e-39 46.094 identity in 256 aa overlap ORF ftt1693c; hypothetical protein 1762452 4200161 FTF1693c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667731.1 1761688 R 393115 CDS YP_667732.1 110671175 4200162 complement(1763093..1763527) 1 NC_008245.1 Similar to Q8ZCK6 Putative membrane protein (Hypothetical) from Yersinia pestis (175 aa). FASTA: opt:353 z-score 450 E():2.9e-17 35.9identity in 142 aa overlap ORF ftt1694c; hypothetical protein 1763527 4200162 FTF1694c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667732.1 1763093 R 393115 CDS YP_667733.1 110671176 4200299 1763778..1764065 1 NC_008245.1 10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring; co-chaperonin GroES 1764065 groES 4200299 groES Francisella tularensis subsp. tularensis FSC198 co-chaperonin GroES YP_667733.1 1763778 D 393115 CDS YP_667734.1 110671177 4200298 1764103..1765737 1 NC_008245.1 60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth; chaperonin GroEL 1765737 groEL 4200298 groEL Francisella tularensis subsp. tularensis FSC198 chaperonin GroEL YP_667734.1 1764103 D 393115 CDS YP_667735.1 110671178 4200184 complement(join(1765953..1766411,1766411..1766737)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1766737 isftu1 4200184 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667735.1 1765953 R 393115 CDS YP_667736.1 110671179 4199406 complement(1766795..1767511) 1 NC_008245.1 Similar to FDH_PSESR Formate dehydrogenase from Pseudomonas sp. (strain 101) (400 aa). FASTA: opt: 1074 z-score: 1253.7, E(): 5.5e-62, 66.949 identity in 236 aa overlap. This CDS is disrupted by the insertion of an ISFtu1 element occurring very near the C-terminal end. It is not clear whether this insertion affects the function of the protein.; formate dehydrogenase 1767511 fdh 4199406 fdh Francisella tularensis subsp. tularensis FSC198 formate dehydrogenase YP_667736.1 1766795 R 393115 CDS YP_667737.1 110671180 4199231 1773101..1775563 1 NC_008245.1 Similar to AAP58968.1 (Q7X3I9) PdpA from Francisella novicida (820 aa). BLAST Score = 1545 bits (4000), Expect = 0.0 Identities = 777/820 (94), Positives = 787/820 (95). Identical to FTF1344. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein 1775563 pdpA2 4199231 pdpA2 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667737.1 1773101 D 393115 CDS YP_667738.1 110671181 4199233 1775568..1778849 1 NC_008245.1 Similar to AAP58967.1 (Q7X3J0) PdpB from Francisella novicida (1093 aa). BLAST Score = 1914 bits (4959), Expect = 0.0 Identities = 975/1093 (89),Positives = 988/1093 (90). This protein is identical to FTF1345. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..140828; hypothetical protein 1778849 pdpB2 4199233 pdpB2 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667738.1 1775568 D 393115 CDS YP_667739.1 110671182 4200163 1778839..1779216 1 NC_008245.1 Identical to FTF1346. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1701; hypothetical protein 1779216 4200163 FTF1701 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667739.1 1778839 D 393115 CDS YP_667740.1 110671183 4200164 1779229..1779723 1 NC_008245.1 Similar to AAP58969.1 (Q7XI38) unknown protein from Francisella novicida (164 aa). BLAST Score = 305 bits (780), Expect = 2e-82 Identities = 163/164 (99),Positives = 163/164 (99). Identical to FTF1347. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1702; hypothetical protein 1779723 4200164 FTF1702 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667740.1 1779229 D 393115 CDS YP_667741.1 110671184 4200165 1779728..1781458 1 NC_008245.1 1-213 aa similar to AAP58970.1 (Q7X3I7) unknown Francisella novicida (212 aa). BLAST Score 352 ,Expect = 1e-95 Identities = 177/209 (84), Positives = 189/209 (90). 330-580 aa similar to AAP58971.1 (Q7X3I6) unknown protein from Francisella tularensis subsp. novicida (251 aa). BLAST Score = 483 bits (1244), Expect = e-135 Identities = 243/251 (96), Positives = 249/251 (99). Identical to FTF1348. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281. Protein fusion product of two hypothetical proteins in F. novicida. ORF ftt1703; hypothetical protein 1781458 4200165 FTF1703 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667741.1 1779728 D 393115 CDS YP_667742.1 110671185 4200166 1781459..1781980 1 NC_008245.1 Similar to AAP58972.1 (Q7X3I5) unknown protein from Francisella novicida (173 aa). BLAST Score = 336, Expect = 1e-91 Identities = 170/173 (98), Positives = 171/173 (98). Identical to FTF1349. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1704; hypothetical protein 1781980 4200166 FTF1704 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667742.1 1781459 D 393115 CDS YP_667743.1 110671186 4200167 1781993..1783423 1 NC_008245.1 Similar to AAP58973.1 (Q7X3I4) unknown protein from Francisella novicida (476 aa). BLAST Score = 864 bits (2233), Expect = 0.0 Identities = 433/476 (90),Positives = 437/476 (91). Identical to FTF1350. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1705; hypothetical protein 1783423 4200167 FTF1705 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667743.1 1781993 D 393115 CDS YP_667744.1 110671187 4200168 1783436..1784059 1 NC_008245.1 Similar to AAP58974.1 (Q7X3I3) unknown protein from Francisella novicida (207 aa). BLAST Score = 391 bits (1005), Expect = e-108 Identities = 194/207 (93),Positives = 195/207 (94). Identical to FTF1351. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1706; hypothetical protein 1784059 4200168 FTF1706 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667744.1 1783436 D 393115 CDS YP_667745.1 110671188 4200169 1784081..1785232 1 NC_008245.1 Similar to AAP58975.1 (Q7X3I2) unknown protein from Francisella novicida (262 aa), BLAST Score = 443 bits (1140), Expect = e-12 Identities = 229/262 (87),Positives = 235/262 (89). Identical to FTF1352. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1707; hypothetical protein 1785232 4200169 FTF1707 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667745.1 1784081 D 393115 CDS YP_667746.1 110671189 4200170 1785198..1785971 1 NC_008245.1 Similar to AAP58976.1 (Q7X3I1) unknown protein from Francisella novicida (257 aa). BLAST Score = 412, Expect = e-114 Identities = 213/257 (82), Positives = 213/257 (82). Identical to FTF1353. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1708; hypothetical protein 1785971 4200170 FTF1708 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667746.1 1785198 D 393115 CDS YP_667747.1 110671190 4199235 1786011..1789997 1 NC_008245.1 Similar to AAP58966.1 PdpC from Francisella novicida (1218 aa). BLAST Score = 2172, Expect = 0.0 Identities = 1089/1220 (89), Positives = 1120/1220 (91),Gaps = 3/1220 (0). Identical to FTF1354. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein 1789997 pdpC2 4199235 pdpC2 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667747.1 1786011 D 393115 CDS YP_667748.1 110671191 4200171 1790015..1790590 1 NC_008245.1 Similar to AAP58977.1 (Q7X3I0) unknown protein from Francisella novicida (188 aa). BLAST Score = 321, Expect = 3e-87 Identities = 156/174 (89), Positives = 162/174 (93). Identical to FTF1355. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281 ORF ftt1710; hypothetical protein 1790590 4200171 FTF1710 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667748.1 1790015 D 393115 CDS YP_667749.1 110671192 4200660 complement(1790597..1791802) 1 NC_008245.1 Similar to AAP58965.1 (Q7C3J6) IglD from Francisella novicida (398 aa). BLAST Score = 746, Expect = 0.0 Identities = 382/398 (95), Positives = 383/398 (96) Identical to FTF1356. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus, subunit D 1791802 iglD2 4200660 iglD2 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit D YP_667749.1 1790597 R 393115 CDS YP_667750.1 110671193 4200658 complement(1791814..1792449) 1 NC_008245.1 Similar to AAP58964.1 (Q7X3J5) IglC from Francisella novicida (23 kDa protein) (211 aa). BLAST Score = 405,Expect = e-112 Identities = 209/211 (99),Positives = 210/211 (99). Identical to FTF1357. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus, subunit C 1792449 iglC2 4200658 iglC2 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit C YP_667750.1 1791814 R 393115 CDS YP_667751.1 110671194 4200656 complement(1792460..1794004) 1 NC_008245.1 Similar to AAP58963.1 (Q7X3J4) IglB from Francisella novicida (514 aa). BLAST Score = 997, Expect = 0.0 Identities = 496/514 (96), Positives = 499/514 (97). Identical to FTF1358. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus, subunit B 1794004 iglB2 4200656 iglB2 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit B YP_667751.1 1792460 R 393115 CDS YP_667752.1 110671195 4200654 complement(1793994..1794584) 1 NC_008245.1 Similar to AAP58962.1 (Q7X3J5) IglA from Francisella novicida (184 aa). BLAST Score = 350, Expect = 6e-96 Identities = 181/184 (98), Positives = 181/184 (98). Identical to FTF1359. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; intracellular growth locus, subunit A 1794584 iglA2 4200654 iglA2 Francisella tularensis subsp. tularensis FSC198 intracellular growth locus, subunit A YP_667752.1 1793994 R 393115 CDS YP_667753.1 110671196 4199237 complement(1794629..1798216) 1 NC_008245.1 Similar to AAP58961.1 (Q7C3J6) PdpD from Francisella novicida (1245 aa). BLAST Score = 2194, Expect = 0.0 Identities = 1133/1245 (91), Positives = 1154/1245 (92), Gaps = 50/1245 (4). Identical to FTF1360. It is part of the duplicated region 1767715..1801625 which is identical to 1374371..1408281; hypothetical protein 1798216 pdpD2 4199237 pdpD2 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667753.1 1794629 R 393115 CDS YP_667754.1 110671197 4200185 complement(join(1800572..1801030,1801030..1801356)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1801356 isftu1 4200185 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667754.1 1800572 R 393115 CDS YP_667755.1 110671198 4199121 complement(1801914..1805786) 1 NC_008245.1 catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis; phosphoribosylformylglycinamidine synthase 1805786 purL 4199121 purL Francisella tularensis subsp. tularensis FSC198 phosphoribosylformylglycinamidine synthase YP_667755.1 1801914 R 393115 CDS YP_667756.1 110671199 4199118 complement(1805786..1807276) 1 NC_008245.1 Catalyzes first step of the de novo purine nucleotide biosynthetic pathway; amidophosphoribosyltransferase 1807276 purF 4199118 purF Francisella tularensis subsp. tularensis FSC198 amidophosphoribosyltransferase YP_667756.1 1805786 R 393115 CDS YP_667757.1 110671200 4199207 complement(1807370..1808716) 1 NC_008245.1 Similar to Q8FHG5 Glutamate decarboxylase beta from E. coli (489 aa). FASTA: opt: 1709 Z-score: 1968.5 bits: 373.6 E(): 8.6e-102 Smith-Waterman score: 1709; 57.011 identity in 435 aa overlap; glutamate decarboxylase 1808716 gad 4199207 gad Francisella tularensis subsp. tularensis FSC198 glutamate decarboxylase YP_667757.1 1807370 R 393115 CDS YP_667758.1 110671201 4199896 complement(1808727..1809371) 1 NC_008245.1 Similar to GSP_BACBR (P40683) 4'-phosphopantetheinyl transferase from Bacillus brevis (237 aa). FASTA: opt: 224 Z-score: 292.3 E(): 2.2e-08 Smith-Waterman score: 224; 33.929 identity in 112 aa overlap ORF ftt1723c; hypothetical protein 1809371 4199896 FTF1723c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667758.1 1808727 R 393115 CDS YP_667759.1 110671202 4199150 complement(1809377..1810906) 1 NC_008245.1 Similar to TOLC_ECOLI (P02930) Outer membrane protein tolC precursor from E. coli (495 aa). FASTA: opt: 548 Z-score: 649.7 E(): 2.7e-28 Smith-Waterman score: 548; 28.000 identity in 425 aa overlap; outer membrane protein tolC 1810906 tolC 4199150 tolC Francisella tularensis subsp. tularensis FSC198 outer membrane protein tolC YP_667759.1 1809377 R 393115 CDS YP_667760.1 110671203 4200098 complement(1810915..1811535) 1 NC_008245.1 Similar to Q9JXU0 Protein-L-isoaspartate O-methyltransferase from Neisseria meningitidis (218 aa). FASTA: opt: 335 Z-score: 438.9 E(): 1.5e-16 Smith-Waterman score: 335; 33.659 identity in 205 aa overlap; protein-L-isoaspartate O-methyltransferase 1811535 pcm 4200098 pcm Francisella tularensis subsp. tularensis FSC198 protein-L-isoaspartate O-methyltransferase YP_667760.1 1810915 R 393115 CDS YP_667761.1 110671204 4199097 1811855..1813186 1 NC_008245.1 Similar to YEGQ_ECOLI (P76403) Putative protease yegQ from E. coli (453 aa). FASTA: opt: 1828 Z-score: 2215.7 E(): 1.6e-115 Smith-Waterman score: 1828; 61.745identity in 447 aa overlap Truncated ortholog (188 aa) previously reported in Schu as O68834 Hypothetical 21.3 kDa protein and AAC12939 unknown; protease yegQ 1813186 yegQ 4199097 yegQ Francisella tularensis subsp. tularensis FSC198 protease yegQ YP_667761.1 1811855 D 393115 CDS YP_667762.1 110671205 4199897 complement(1813227..1814417) 1 NC_008245.1 Similar to Q83CE1 Drug resistance transporter,Bcr/CflA family from Coxiella burnetii (398 aa). FASTA: opt: 957 Z-score: 1006.0 E(): 3.8e-48 Smith-Waterman score: 957; 38.542identity in 384 aa overlap ORF ftt1727c; multidrug resistance protein, membrane located 1814417 4199897 FTF1727c Francisella tularensis subsp. tularensis FSC198 multidrug resistance protein, membrane located YP_667762.1 1813227 R 393115 CDS YP_667763.1 110671206 4200241 1814583..1816010 1 NC_008245.1 Similar to Q8D4S6 Na+/H+ antiporter NhaD from Vibrio vulnificus (478 aa). FASTA: opt: 1428 Z-score: 1679.2 E(): 1.2e-85 Smith-Waterman score: 1592; 50.617identity in 486 aa overlap; Na+/H+ antiporter 1816010 nhaD 4200241 nhaD Francisella tularensis subsp. tularensis FSC198 Na+/H+ antiporter YP_667763.1 1814583 D 393115 CDS YP_667764.1 110671207 4199898 complement(1817661..1819535) 1 NC_008245.1 Similar to Q97MC6 Amino acid transporter from Clostridium acetobutylicum (620 aa). FASTA: opt: 1338 Z-score: 1514.2 E(): 1.9e-76 Smith-Waterman score: 1338; 37.745 identity in 612 aa overlap ORF ftt1730c; amino acid transporter 1819535 4199898 FTF1730c Francisella tularensis subsp. tularensis FSC198 amino acid transporter YP_667764.1 1817661 R 393115 CDS YP_667765.1 110671208 4199899 complement(1819542..1820546) 1 NC_008245.1 essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication; GTPase ObgE 1820546 obgE 4199899 obgE Francisella tularensis subsp. tularensis FSC198 GTPase ObgE YP_667765.1 1819542 R 393115 CDS YP_667766.1 110671209 4199900 complement(1820622..1821818) 1 NC_008245.1 Similar to Q8FGM8 Tyrosine-specific transport protein from E. coli (403 aa). FASTA: opt: 724 Z-score: 839.2 E(): 6.8e-39 Smith-Waterman score: 724; 35.638 identity in 376 aa overlap ORF ftt1732; aromatic amino acid HAAP transporter 1821818 4199900 FTF1732c Francisella tularensis subsp. tularensis FSC198 aromatic amino acid HAAP transporter YP_667766.1 1820622 R 393115 CDS YP_667767.1 110671210 4199901 1821945..1822256 1 NC_008245.1 ORF ftt1733; hypothetical protein 1822256 4199901 FTF1733 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667767.1 1821945 D 393115 CDS YP_667768.1 110671211 4199504 complement(1823837..1826518) 1 NC_008245.1 Similar to Q8U9E1 Two component sensor kinase from Agrobacterium tumefaciens (900 aa). FASTA: opt: 1625 Z-score: 1777.7 E(): 3.6e-91 Smith-Waterman score: 1625; 32.745 identity in 907 aa overlap; two component sensor protein kdpD 1826518 kdpD 4199504 kdpD Francisella tularensis subsp. tularensis FSC198 two component sensor protein kdpD YP_667768.1 1823837 R 393115 CDS YP_667769.1 110671212 4199503 complement(1826622..1827176) 1 NC_008245.1 Similar to Q8R8I7 K+-transporting ATPase, c chain from Thermoanaerobacter tengcogenesis (193 aa). FASTA: opt: 404 Z-score: 471.4 E(): 2.1e-18 Smith-Waterman score: 404; 43.333identity in 180 aa overlap; potassium-transporting ATPase C chain 1827176 kdpC 4199503 kdpC Francisella tularensis subsp. tularensis FSC198 potassium-transporting ATPase C chain YP_667769.1 1826622 R 393115 CDS YP_667770.1 110671213 4199502 complement(1827193..1829232) 1 NC_008245.1 Similar to ATKB_RHIME (Q92XJ0) Potassium-transporting ATPase B chain from Rhizobium meliloti (680 aa). FASTA: opt: 2393 Z-score: 2638.7 E(): 4.4e-139 Smith-Waterman score: 2393; 55.490 identity in 683 aa overlap; potassium-transporting ATPase B chain 1829232 kdpB 4199502 kdpB Francisella tularensis subsp. tularensis FSC198 potassium-transporting ATPase B chain YP_667770.1 1827193 R 393115 CDS YP_667771.1 110671214 4200186 complement(join(1831691..1832149,1832149..1832475)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1832475 isftu1 4200186 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667771.1 1831691 R 393115 CDS YP_667772.1 110671215 4199612 complement(1833212..1834909) 1 NC_008245.1 Similar to Q8TT45 Indolepyruvate decarboxylase Methanosarcina activorans (550 aa). FASTA: opt: 1265 Z-score: 1467.5 E(): 7.6e-74 Smith-Waterman score: 1265; 36.909 identity in 550 aa overlap; indolepyruvate decarboxylase 1834909 ipdC 4199612 ipdC Francisella tularensis subsp. tularensis FSC198 indolepyruvate decarboxylase YP_667772.1 1833212 R 393115 CDS YP_667773.1 110671216 4199906 1835440..1836363 1 NC_008245.1 Similar to Q87YG1 Signal peptide peptidase SppA,36K type from Pseudomonas syringae (332 a). FASTA: opt: 839 Z-score: 1007.2 E(): 3.3e-48 Smith-Waterman score: 839; 46.622 identity in 296 aa overlap ORF ftt1746; peptidase 1836363 4199906 FTF1746 Francisella tularensis subsp. tularensis FSC198 peptidase YP_667773.1 1835440 D 393115 CDS YP_667774.1 110671217 4199907 1836417..1836992 1 NC_008245.1 Similar to AAO90156 (Q83DT1) Outer membrane protein OmpH, putative, from Coxiella burnetii (165 aa). FASTA: opt: 175 Z-score: 183.6 E(): 0.022 Smith-Waterman score: 216; 26.816 identity in 179 aa overlap ORF ftt1747; outer membrane protein 1836992 4199907 FTF1747 Francisella tularensis subsp. tularensis FSC198 outer membrane protein YP_667774.1 1836417 D 393115 CDS YP_667775.1 110671218 4199794 1837128..1837550 1 NC_008245.1 Similar to AAO91018 (Q83BI7) Rhodanese domain protein Coxiella burnetii (144 aa). FASTA: opt: 279 Z-score: 342.5 E(): 3.5e-11 Smith-Waterman score: 279; 33.094 identity in 139 aa overlap; thiosulfate sulfurtransferase 1837550 glpe 4199794 glpe Francisella tularensis subsp. tularensis FSC198 thiosulfate sulfurtransferase YP_667775.1 1837128 D 393115 CDS YP_667776.1 110671219 4200734 1837577..1838026 1 NC_008245.1 molecular chaperone that is required for the normal export of envelope proteins out of the cell cytoplasm; in Escherichia coli this proteins forms a homotetramer in the cytoplasm and delivers proteins to be exported to SecA; preprotein translocase subunit SecB 1838026 secB1 4200734 secB1 Francisella tularensis subsp. tularensis FSC198 preprotein translocase subunit SecB YP_667776.1 1837577 D 393115 CDS YP_667777.1 110671220 4200599 1838048..1839127 1 NC_008245.1 catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs; recombinase A 1839127 recA 4200599 recA Francisella tularensis subsp. tularensis FSC198 recombinase A YP_667777.1 1838048 D 393115 CDS YP_667778.1 110671221 4200608 1839114..1839566 1 NC_008245.1 Similar to RECX_HAEIN (P43706) Regulatory protein recX from Haemophilus influenzae (126 aa). FASTA: opt: 243 Z-score: 314.3 E(): 1.3e-09 Smith-Waterman score: 243; 31.967 identity in 122 aa overlap; regulatory protein recX 1839566 recX 4200608 recX Francisella tularensis subsp. tularensis FSC198 regulatory protein recX YP_667778.1 1839114 D 393115 CDS YP_667779.1 110671222 4199084 1839739..1840215 1 NC_008245.1 Similar to SSB_HAEIN P44409 Single-strand binding protein (SSB) from Haemophilus influenzae (168 aa). FASTA: opt: 437 Z-score: 543.8 E(): 1.9e-22 Smith-Waterman score: 437; 44.578identity in 166 aa overlap; single-strand binding protein 1840215 ssb 4199084 ssb Francisella tularensis subsp. tularensis FSC198 single-strand binding protein YP_667779.1 1839739 D 393115 CDS YP_667780.1 110671223 4199134 1840321..1841475 1 NC_008245.1 Similar to ACKA_CLOTS (Q59331) Acetate kinase (Acetokinase) from Clostridium thermosaccharolyticum (400 aa). FASTA: opt: 1204 Z-score: 1394.5 E(): 8e-70 Smith-Waterman score: 1204; 49.873 identity in 395 aa overlap; propionate kinase 1841475 tdcD 4199134 tdcD Francisella tularensis subsp. tularensis FSC198 propionate kinase YP_667780.1 1840321 D 393115 CDS YP_667781.1 110671224 4199947 1841485..1843581 1 NC_008245.1 catalyzes the synthesis of acetylphosphate or propionylphosphate from acetyl-CoA or propionyl-CoA and inorganic phosphate; when using propionyl-CoA the enzyme is functioning in the anaerobic pathway catabolizing threonine to propionate; phosphate acetyltransferase 1843581 pta 4199947 pta Francisella tularensis subsp. tularensis FSC198 phosphate acetyltransferase YP_667781.1 1841485 D 393115 CDS YP_667782.1 110671225 4200187 complement(join(1844589..1845047,1845047..1845373)) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1845373 isftu1 4200187 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667782.1 1844589 R 393115 CDS YP_667783.1 110671226 4199909 complement(1846810..1847403) 1 NC_008245.1 Similar to Q8D0W2 Putative cytochrome from Yersinia pestis (199 aa). FASTA: opt: 273 Z-score: 328.7 E(): 1.9e-10 Smith-Waterman score: 273; 27.979identity in 193 aa overlap ORF ftt1758c; B-type cytochrome 1847403 4199909 FTF1758c Francisella tularensis subsp. tularensis FSC198 B-type cytochrome YP_667783.1 1846810 R 393115 CDS YP_667784.1 110671227 4200240 1848371..1849522 1 NC_008245.1 exports sodium by using the electrochemical proton gradient to allow protons into the cell; functions in adaptation to high salinity and alkaline pH; activity increases at higher pH; downregulated at acidic pH; pH-dependent sodium/proton antiporter 1849522 nhaA 4200240 nhaA Francisella tularensis subsp. tularensis FSC198 pH-dependent sodium/proton antiporter YP_667784.1 1848371 D 393115 CDS YP_667785.1 110671228 4199911 1849587..1850306 1 NC_008245.1 Similar to YAFD_ECOLI (P30865) Hypothetical protein yafD from E. coli (266 aa). FASTA: opt: 320 Z-score: 403.2 E(): 1.5e-14 Smith-Waterman score: 320; 34.498 identity in 229 aa overlap ORF ftt1761; hypothetical protein 1850306 4199911 FTF1761 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667785.1 1849587 D 393115 CDS YP_667786.1 110671229 4199535 complement(1850312..1851052) 1 NC_008245.1 Similar to Q9F728 Putative N-acylhomoserine lactone synthase from Pseudomonas fluorescens (259 aa). FASTA: opt: 408 Z-score: 521.4 E(): 3.8e-21 Smith-Waterman score: 408; 34.498 identity in 229 aa overlap ORF ftt1762c; acetyltransferase protein 1851052 4199535 FTF1762c Francisella tularensis subsp. tularensis FSC198 acetyltransferase protein YP_667786.1 1850312 R 393115 CDS YP_667787.1 110671230 4199536 complement(1851066..1851818) 1 NC_008245.1 Similar to Q9F728 Putative N-acylhomoserine lactone syntase from Pseudomonas fluorescens (259 aa). FASTA: opt: 442 Z-score: 546.9 E(): 1.4e-22 Smith-Waterman score: 442; 37.963 identity in 216 aa overlap. ORF ftt1763c; acetyltransferase protein 1851818 4199536 FTF1763c Francisella tularensis subsp. tularensis FSC198 acetyltransferase protein YP_667787.1 1851066 R 393115 CDS YP_667788.1 110671231 4199537 complement(1851981..1852304) 1 NC_008245.1 Similar to FER_PSEST (P08811) Ferredoxin from Pseudomonas stutzeri (106 aa). FASTA: opt: 535 Z-score: 674.6 E(): 1.1e-29 Smith-Waterman score: 535; 65.714identity in 105 aa overlap ORF ftt1764c; ferredoxin 1852304 4199537 FTF1764c Francisella tularensis subsp. tularensis FSC198 ferredoxin YP_667788.1 1851981 R 393115 CDS YP_667789.1 110671232 4199538 1852524..1853177 1 NC_008245.1 ORF ftt1765; hypothetical protein 1853177 4199538 FTF1765 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667789.1 1852524 D 393115 CDS YP_667790.1 110671233 4199539 1853255..1853911 1 NC_008245.1 Similar to Q8YLW7 O-methyltransferase from Anabaena sp. (220 aa). FASTA: opt: 622 Z-score: 791.6 E(): 3.4e-36 Smith-Waterman score: 622; 43.836identity in 219 aa overlap ORF ftt1766; O-methyltransferase 1853911 4199539 FTF1766 Francisella tularensis subsp. tularensis FSC198 O-methyltransferase YP_667790.1 1853255 D 393115 CDS YP_667791.1 110671234 4199124 complement(1853913..1855073) 1 NC_008245.1 non-folate utilizing enzyme, catalyzes the production of beta-formyl glycinamide ribonucleotide from formate, ATP, and beta-GAR and a side reaction producing acetyl phosphate and ADP from acetate and ATP; involved in de novo purine biosynthesis; phosphoribosylglycinamide formyltransferase 2 1855073 purT 4199124 purT Francisella tularensis subsp. tularensis FSC198 phosphoribosylglycinamide formyltransferase 2 YP_667791.1 1853913 R 393115 CDS YP_667792.1 110671235 4199540 complement(1855127..1856947) 1 NC_008245.1 Similar to Q9KQP6 Chitinase from Vibrio cholerae (846 aa). FASTA: opt: 242 Z-score: 272.3 E(): 2.8e-07 Smith-Waterman score: 279; 21.309 identity in 718 aa overlap. ORF ftt1768c; chitinase 1856947 4199540 FTF1768c Francisella tularensis subsp. tularensis FSC198 chitinase YP_667792.1 1855127 R 393115 CDS YP_667793.1 110671236 4200323 complement(1857569..1860148) 1 NC_008245.1 Similar to FASTA: AAO89660 (Q83F55) ClpB protein from Coxiella burnetii (859 aa). FASTA: opt: 3593 Z-score: 3223.6 E(): 1.1e-171 Smith-Waterman score: 3593; 65.357 identity in 840 aa overlap; ClpB protein 1860148 clpB 4200323 clpB Francisella tularensis subsp. tularensis FSC198 ClpB protein YP_667793.1 1857569 R 393115 CDS YP_667794.1 110671237 4199542 1861167..1861520 1 NC_008245.1 ORF ftt1771; hypothetical protein 1861520 4199542 FTF1771 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667794.1 1861167 D 393115 CDS YP_667795.1 110671238 4200716 complement(1861575..1862384) 1 NC_008245.1 catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis; tryptophan synthase subunit alpha 1862384 trpA 4200716 trpA Francisella tularensis subsp. tularensis FSC198 tryptophan synthase subunit alpha YP_667795.1 1861575 R 393115 CDS YP_667796.1 110671239 4200717 complement(1862384..1863574) 1 NC_008245.1 catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate; tryptophan synthase subunit beta 1863574 trpB 4200717 trpB Francisella tularensis subsp. tularensis FSC198 tryptophan synthase subunit beta YP_667796.1 1862384 R 393115 CDS YP_667797.1 110671240 4200322 complement(1864990..1866519) 1 NC_008245.1 Similar to CLCA_ECOLI (P37019) Voltage-gated ClC-type chloride channel from E. coli (473 aa). FASTA: opt: 1120 Z-score: 1211.7 E(): 1.3e-59 Smith-Waterman score: 1120; 39.545 identity in 440 aa overlap; voltage-gated ClC-type chloride channel clcA 1866519 clcA 4200322 clcA Francisella tularensis subsp. tularensis FSC198 voltage-gated ClC-type chloride channel clcA YP_667797.1 1864990 R 393115 CDS YP_667798.1 110671241 4199544 complement(1866590..1866988) 1 NC_008245.1 ORF ftt1776c; hypothetical protein 1866988 4199544 FTF1776c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667798.1 1866590 R 393115 CDS YP_667799.1 110671242 4199545 complement(1866991..1867401) 1 NC_008245.1 ORF ftt1777c; hypothetical protein 1867401 4199545 FTF1777c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667799.1 1866991 R 393115 CDS YP_667800.1 110671243 4199546 complement(1867422..1867814) 1 NC_008245.1 ORF ftt1778c; hypothetical protein 1867814 4199546 FTF1778c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667800.1 1867422 R 393115 CDS YP_667801.1 110671244 4200188 join(1868647..1868973,1868973..1869431) 1 NC_008245.1 ISFtu1. Transposase, member of the IS630 Tc-1 mariner family. Identical to Q93EJ6 Putative transposase from Francisella tularensis (118 aa) from aa 144-261. Identical to Q93EJ7 putative transposase from Francisella tularensis (126 aa) from aa 1-109. Q93EJ6 and Q93EJ7 previously characterised as two separate ORF's. ORF1 (identical to Q93EJ7) contains a programmed ribosomal frameshifting motif, 5'-AAAAAAAG-3' at aa position 109 which permits frameshifting to ORF2 (identical to Q93EJ6) to produce a single ORF. Alignment of this single ORF with additional members of the IS630 family, and also mariner elements, show that this transposase shares strongly conserved residues, including a fully conserved dipeptide,Asp-Glu (DE), and a block consisting of a fully conserved Asp and highly conserved Glu, separated by 34 or 35 residues (D35E). The conserved dipeptide is only present if ribosomal frameshifting occurs.; transposase 1869431 isftu1 4200188 isftu1 Francisella tularensis subsp. tularensis FSC198 transposase YP_667801.1 1868647 D 393115 CDS YP_667802.1 110671245 4199548 complement(1869670..1870419) 1 NC_008245.1 ORF ftt1781c; hypothetical protein 1870419 4199548 FTF1781c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667802.1 1869670 R 393115 CDS YP_667803.1 110671246 4199031 complement(1870361..1872040) 1 NC_008245.1 ChvD; in Agrobacterium tumefaciens, mutations in both Walker boxes were found to affect virulence; putative ABC transporter ATP-binding protein 1872040 yjjK 4199031 yjjK Francisella tularensis subsp. tularensis FSC198 putative ABC transporter ATP-binding protein YP_667803.1 1870361 R 393115 CDS YP_667804.1 110671247 4199549 1872208..1873494 1 NC_008245.1 Similar to Q83A52 Major facilitator family transporter from Coxiella burnetii (443 aa). FASTA: opt: 751 Z-score: 809.6 E(): 3.3e-37 Smith-Waterman score: 751; 34.204identity in 421 aa overlap ORF ftt1783; major facilitator transporter 1873494 4199549 FTF1783 Francisella tularensis subsp. tularensis FSC198 major facilitator transporter YP_667804.1 1872208 D 393115 CDS YP_667805.1 110671248 4199550 complement(1873475..1873783) 1 NC_008245.1 ORF ftt1784c; hypothetical protein 1873783 4199550 FTF1784c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667805.1 1873475 R 393115 CDS YP_667806.1 110671249 4199551 complement(1873795..1874007) 1 NC_008245.1 ORF ftt1785c; hypothetical protein 1874007 4199551 FTF1785c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667806.1 1873795 R 393115 CDS YP_667807.1 110671250 4199988 complement(1875041..1875652) 1 NC_008245.1 Similar to Q8UGV8 LysE family transporter from Agrobactium tumifaciens (202 aa). FASTA: opt: 346 Z-score: 414.9 E(): 3.2e-15 Smith-Waterman score: 346; 30.102 identity in 196 aa overlap ORF ftt1787c; amino acid transporter LysE 1875652 4199988 FTF1787c Francisella tularensis subsp. tularensis FSC198 amino acid transporter LysE YP_667807.1 1875041 R 393115 CDS YP_667808.1 110671251 4199989 1876953..1877747 1 NC_008245.1 Similar to Q83C04 Membrane protein, putative, from Coxiella burnetii (263 aa). FASTA: opt: 496 Z-score: 540.4 E(): 3.3e-22 Smith-Waterman score: 496; 30.038 identity in 263 aa overlap ORF ftt1789; hypothetical protein 1877747 4199989 FTF1789 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667808.1 1876953 D 393115 CDS YP_667809.1 110671252 4199991 1878156..1878518 1 NC_008245.1 ORF ftt1791; hypothetical protein 1878518 4199991 FTF1791 Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667809.1 1878156 D 393115 CDS YP_667810.1 110671253 4199240 complement(1879650..1882244) 1 NC_008245.1 Similar to AMPN_ECOLI (P04825) Aminopeptidase N from E. coli (869 aa). FASTA: opt: 2226 Z-score: 2532.9 E(): 3.4e-133 Smith-Waterman score: 2751; 48.856identity in 874 aa overlap; aminopeptidase N 1882244 pepN 4199240 pepN Francisella tularensis subsp. tularensis FSC198 aminopeptidase N YP_667810.1 1879650 R 393115 CDS YP_667811.1 110671254 4199993 1882428..1882856 1 NC_008245.1 Similar to Q9WYK7 Heat shock protein, class I from Thermatoga maritima (147 aa). FASTA: opt: 288 Z-score: 346.4 E(): 2.1e-11 Smith-Waterman score: 288; 37.879 identity in 132 aa overlap ORF ftt1794; heat shock protein 1882856 4199993 FTF1794 Francisella tularensis subsp. tularensis FSC198 heat shock protein YP_667811.1 1882428 D 393115 CDS YP_667812.1 110671255 4200718 complement(1882853..1884214) 1 NC_008245.1 monomeric bifunctional protein; functions in tryptophan biosynthesis pathway; phosphoribosylanthranilate is rearranged to carboxyphenylaminodeoxyribulosephosphate which is then closed to form indole-3-glycerol phosphate; bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase 1884214 trpC 4200718 trpC Francisella tularensis subsp. tularensis FSC198 bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase YP_667812.1 1882853 R 393115 CDS YP_667813.1 110671256 4199994 complement(1884305..1884718) 1 NC_008245.1 Similar to Q9KF27 Hypothetical protein BH0670 from Bacillus halodurans (159 aa). FASTA: opt: 208 Z-score: 278.0 E(): 1.4e-07 Smith-Waterman score: 208; 28.682 identity in 129 aa overlap ORF ftt1796c; hypothetical protein 1884718 4199994 FTF1796c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667813.1 1884305 R 393115 CDS YP_667814.1 110671257 4200101 complement(1884723..1885412) 1 NC_008245.1 Similar to MSA2_CAUCR (Q9A9E9) Peptide methionine sulfoxide reductase from Caulobacter crescentus (196 aa). FASTA opt: 510 Z-score: 598.2 E(): 2e-25 Smith-Waterman score: 510; 39.024 identity in 205 aa overlap; peptide methionine sulfoxide reductase MsrA 1885412 msrA2 4200101 msrA2 Francisella tularensis subsp. tularensis FSC198 peptide methionine sulfoxide reductase MsrA YP_667814.1 1884723 R 393115 CDS YP_667815.1 110671258 4199995 complement(1885496..1885864) 1 NC_008245.1 ORF ftt1798c; hypothetical protein 1885864 4199995 FTF1798c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667815.1 1885496 R 393115 CDS YP_667816.1 110671259 4199997 complement(1886267..1886719) 1 NC_008245.1 ORF ftt1800c; hypothetical protein 1886719 4199997 FTF1800c Francisella tularensis subsp. tularensis FSC198 hypothetical protein YP_667816.1 1886267 R 393115 CDS YP_667817.1 110671260 4200719 complement(1888373..1889917) 1 NC_008245.1 with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine; anthranilate synthase component I 1889917 trpE 4200719 trpE Francisella tularensis subsp. tularensis FSC198 anthranilate synthase component I YP_667817.1 1888373 R 393115 CDS YP_667818.1 110671261 4200722 complement(1889926..1890213) 1 NC_008245.1 Similar to TRPR_ECOLI (P03032) Trp operon repressor from E. coli (107 aa). FASTA: opt: 175 Z-score: 239.1 E(): 2e-05 Smith-Waterman score: 175; 32.584 identity in 89 aa overlap; trp operon repressor 1890213 trpR 4200722 trpR Francisella tularensis subsp. tularensis FSC198 trp operon repressor YP_667818.1 1889926 R 393115 CDS YP_667819.1 110671262 4200765 complement(1890687..1892183) 1 NC_008245.1 Similar to RNG_ECOLI (P25537) Ribonuclease G from E. coli (488 aa). FASTA: opt: 1439 Z-score: 1642.1 E(): 1.4e-83 Smith-Waterman score: 1439; 45.418 identity in 491 aa overlap; ribonuclease G 1892183 rng 4200765 rng Francisella tularensis subsp. tularensis FSC198 ribonuclease G YP_667819.1 1890687 R 393115 CDS