-- dump date 20111111_072853 -- class Genbank::Feature -- table feature -- table main -- field 1 id -- field 2 type -- field 3 name -- field 4 contig -- field 5 start_pos -- field 6 end_pos -- field 7 strand -- field 8 description -- field 9 chrom_position -- field 10 organism -- field 11 GeneID -- header -- id type name contig start_pos end_pos strand description chrom_position organism GeneID 4932000001 SEQ_END SEQ_END NC_001133.7 230208 230208 DR NC_001133.7; contig end 230208..230208 Saccharomyces cerevisiae 4932000002 SEQ_END SEQ_END NC_001134.7 813178 813178 DR NC_001134.7; contig end 813178..813178 Saccharomyces cerevisiae 4932000003 SEQ_END SEQ_END NC_001135.4 316617 316617 DR NC_001135.4; contig end 316617..316617 Saccharomyces cerevisiae 4932000004 SEQ_END SEQ_END NC_001136.8 1531918 1531918 DR NC_001136.8; contig end 1531918..1531918 Saccharomyces cerevisiae 4932000005 SEQ_END SEQ_END NC_001141.1 439885 439885 DR NC_001141.1; contig end 439885..439885 Saccharomyces cerevisiae 4932000006 SEQ_END SEQ_END NC_001224.1 85779 85779 DR NC_001224.1; contig end 85779..85779 Saccharomyces cerevisiae 4932000007 SEQ_END SEQ_END NC_001137.2 576869 576869 DR NC_001137.2; contig end 576869..576869 Saccharomyces cerevisiae 4932000008 SEQ_END SEQ_END NC_001138.4 270148 270148 DR NC_001138.4; contig end 270148..270148 Saccharomyces cerevisiae 4932000009 SEQ_END SEQ_END NC_001139.8 1090947 1090947 DR NC_001139.8; contig end 1090947..1090947 Saccharomyces cerevisiae 4932000010 SEQ_END SEQ_END NC_001140.5 562643 562643 DR NC_001140.5; contig end 562643..562643 Saccharomyces cerevisiae 4932000011 SEQ_END SEQ_END NC_001142.7 745745 745745 DR NC_001142.7; contig end 745745..745745 Saccharomyces cerevisiae 4932000012 SEQ_END SEQ_END NC_001143.7 666454 666454 DR NC_001143.7; contig end 666454..666454 Saccharomyces cerevisiae 4932000013 SEQ_END SEQ_END NC_001144.4 1078175 1078175 DR NC_001144.4; contig end 1078175..1078175 Saccharomyces cerevisiae 4932000014 SEQ_END SEQ_END NC_001145.2 924429 924429 DR NC_001145.2; contig end 924429..924429 Saccharomyces cerevisiae 4932000015 SEQ_END SEQ_END NC_001146.6 784333 784333 DR NC_001146.6; contig end 784333..784333 Saccharomyces cerevisiae 4932000016 SEQ_END SEQ_END NC_001147.5 1091289 1091289 DR NC_001147.5; contig end 1091289..1091289 Saccharomyces cerevisiae 4932000017 SEQ_END SEQ_END NC_001148.3 948062 948062 DR NC_001148.3; contig end 948062..948062 Saccharomyces cerevisiae NP_009332.1 CDS PAU8 NC_001133.7 1807 2169 R Pau8p complement(1807..2169) Saccharomyces cerevisiae 851229 NP_878038.1 CDS YAL067W-A NC_001133.7 2480 2707 D Yal067w-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 2480..2707 Saccharomyces cerevisiae 1466426 NP_009333.1 CDS SEO1 NC_001133.7 7236 9017 R Seo1p; Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide complement(7236..9017) Saccharomyces cerevisiae 851230 NP_009335.1 CDS YAL065C NC_001133.7 11566 11952 R Yal065cp; Putative protein of unknown function; has homology to FLO1; possible pseudogene complement(11566..11952) Saccharomyces cerevisiae 851232 NP_009336.1 CDS YAL064W-B NC_001133.7 12047 12427 D Yal064w-bp; Fungal-specific protein of unknown function 12047..12427 Saccharomyces cerevisiae 851233 NP_058136.1 CDS YAL064C-A NC_001133.7 13364 13744 R Yal064c-ap; Putative protein of unknown function; YAL064C-A is not an essential gene complement(13364..13744) Saccharomyces cerevisiae 851234 NP_009337.1 CDS YAL064W NC_001133.7 21526 21852 D Yal064wp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments 21526..21852 Saccharomyces cerevisiae 851235 NP_878039.1 CDS YAL063C-A NC_001133.7 22397 22687 R Yal063c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(22397..22687) Saccharomyces cerevisiae 1466427 NP_009338.1 CDS FLO9 NC_001133.7 24001 27969 R Lectin-like protein with similarity to Flo1p, thought to be expressed and involved in flocculation; Flo9p complement(24001..27969) Saccharomyces cerevisiae 851236 NP_009339.1 CDS GDH3 NC_001133.7 31568 32941 D NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; Gdh3p 31568..32941 Saccharomyces cerevisiae 851237 NP_009340.1 CDS BDH2 NC_001133.7 33449 34702 D Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3; BDH2 is an essential gene; Bdh2p 33449..34702 Saccharomyces cerevisiae 851238 NP_009341.1 CDS BDH1 NC_001133.7 35156 36304 D NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source; Bdh1p 35156..36304 Saccharomyces cerevisiae 851239 NP_009342.1 CDS ECM1 NC_001133.7 36510 37148 D Protein of unknown function, localized in the nucleoplasm and the nucleolus, genetically interacts with MTR2 in 60S ribosomal protein subunit export; Ecm1p 36510..37148 Saccharomyces cerevisiae 851240 NP_009343.1 CDS CNE1 NC_001133.7 37465 38973 D Cne1p; Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast 37465..38973 Saccharomyces cerevisiae 851241 NP_009345.2 CDS GPB2 NC_001133.7 39260 41902 D Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb1p; Gpb2p 39260..41902 Saccharomyces cerevisiae 851243 NP_009346.1 CDS PEX22 NC_001133.7 42178 42720 D Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation; Pex22p 42178..42720 Saccharomyces cerevisiae 851244 NP_009347.1 CDS ACS1 NC_001133.7 42882 45023 R Acs1p; Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetlyation; expressed during growth on nonfermentable carbon sources and under aerobic conditions complement(42882..45023) Saccharomyces cerevisiae 851245 NP_009348.1 CDS FLC2 NC_001133.7 45900 48251 D Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; Flc2p 45900..48251 Saccharomyces cerevisiae 851246 NP_009349.2 CDS OAF1 NC_001133.7 48565 51708 D Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; Oaf1p 48565..51708 Saccharomyces cerevisiae 851247 NP_009350.1 CDS AIM2 NC_001133.7 51856 52596 R Cytoplasmic protein of unknown function, potential Hsp82p interactor; null mutant displays decreased frequency of mitochondrial genome loss (petite formation); Aim2p complement(51856..52596) Saccharomyces cerevisiae 851248 NP_009351.1 CDS GEM1 NC_001133.7 52802 54790 R Gem1p; Evolutionarily-conserved tail-anchored outer mitochondrial membrane GTPase which regulates mitochondrial morphology; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; not required for pheromone-induced cell death complement(52802..54790) Saccharomyces cerevisiae 851249 NP_009352.1 CDS SPC72 NC_001133.7 54990 56858 R Spc72p; Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization complement(54990..56858) Saccharomyces cerevisiae 851250 NP_009353.1 CDS AIM1 NC_001133.7 57030 57386 R Putative protein of unknown function; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim1p complement(57030..57386) Saccharomyces cerevisiae 851251 NP_075206.1 CDS YAL044W-A NC_001133.7 57519 57851 D Yal044w-ap; Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein 57519..57851 Saccharomyces cerevisiae 851252 NP_009355.2 CDS GCV3 NC_001133.7 57951 58463 R H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm; Gcv3p complement(57951..58463) Saccharomyces cerevisiae 851254 NP_009356.1 CDS PTA1 NC_001133.7 58696 61053 R Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII; Pta1p complement(58696..61053) Saccharomyces cerevisiae 851255 NP_009358.1 CDS ERV46 NC_001133.7 61317 62564 D Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport; Erv46p 61317..62564 Saccharomyces cerevisiae 851256 NP_009359.1 CDS CDC24 NC_001133.7 62841 65405 D Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; Cdc24p 62841..65405 Saccharomyces cerevisiae 851190 NP_009360.1 CDS CLN3 NC_001133.7 65779 67521 R G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; Cln3p complement(65779..67521) Saccharomyces cerevisiae 851191 NP_009361.1 CDS CYC3 NC_001133.7 68717 69526 R Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS); Cyc3p complement(68717..69526) Saccharomyces cerevisiae 851192 NP_009362.1 CDS CDC19 NC_001133.7 71787 73289 D Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; Cdc19p 71787..73289 Saccharomyces cerevisiae 851193 NP_878040.1 CDS YAL037C-A NC_001133.7 73427 73519 R Yal037c-ap; Putative protein of unknown function complement(73427..73519) Saccharomyces cerevisiae 1466428 NP_009363.1 CDS YAL037W NC_001133.7 74021 74824 D Yal037wp; Putative protein of unknown function 74021..74824 Saccharomyces cerevisiae 851194 NP_009364.1 CDS RBG1 NC_001133.7 75044 76153 R Rbg1p; Member of the DRG family of GTP-binding proteins; interacts with translating ribosomes and with Tma46p complement(75044..76153) Saccharomyces cerevisiae 851195 NP_009365.1 CDS FUN12 NC_001133.7 76428 79436 D GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2; Fun12p 76428..79436 Saccharomyces cerevisiae 851196 NP_009367.1 CDS MTW1 NC_001133.7 79719 80588 D Mtw1p; Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly 79719..80588 Saccharomyces cerevisiae 851197 NP_009368.2 CDS FUN19 NC_001133.7 80711 81952 R Non-essential protein of unknown function; Fun19p complement(80711..81952) Saccharomyces cerevisiae 851199 NP_009369.1 CDS POP5 NC_001133.7 82707 83228 D Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Pop5p 82707..83228 Saccharomyces cerevisiae 851200 NP_009370.1 CDS PRP45 NC_001133.7 83336 84475 R Prp45p; Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene complement(83336..84475) Saccharomyces cerevisiae 851201 NP_009371.1 CDS GIP4 NC_001133.7 84750 87032 R Gip4p; Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate complement(84750..87032) Saccharomyces cerevisiae 851202 NP_009372.1 CDS SNC1 NC_001133.7 87287 87753 D Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; Snc1p join(87287..87388,87502..87753) Saccharomyces cerevisiae 851203 NP_009373.1 CDS MYO4 NC_001133.7 87856 92271 R One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; Myo4p complement(87856..92271) Saccharomyces cerevisiae 851204 NP_009374.1 CDS FRT2 NC_001133.7 92901 94487 D Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike Frt1p), promotes growth in conditions of high Na+, alkaline pH, or cell wall stress; potential Cdc28p substrate; Frt2p 92901..94487 Saccharomyces cerevisiae 851205 NP_009375.1 CDS SAW1 NC_001133.7 94688 95473 D Saw1p; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene 94688..95473 Saccharomyces cerevisiae 851206 NP_009376.1 CDS DRS2 NC_001133.7 95631 99698 R Drs2p; Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vessicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease complement(95631..99698) Saccharomyces cerevisiae 851207 NP_009377.1 CDS MAK16 NC_001133.7 100226 101146 R Mak16p; Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus complement(100226..101146) Saccharomyces cerevisiae 851208 NP_009378.1 CDS LTE1 NC_001133.7 101566 105873 R Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP; Lte1p complement(101566..105873) Saccharomyces cerevisiae 851209 NP_009379.2 CDS PMT2 NC_001133.7 106273 108552 R Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt1p, can instead interact with Pmt5p in some conditions; target for new antifungals; Pmt2p complement(106273..108552) Saccharomyces cerevisiae 851210 NP_009380.1 CDS FUN26 NC_001133.7 108878 110431 R Nucleoside transporter with broad nucleoside selectivity; localized to intracellular membranes; Fun26p complement(108878..110431) Saccharomyces cerevisiae 851211 NP_009381.1 CDS CCR4 NC_001133.7 110847 113360 R Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening; Ccr4p complement(110847..113360) Saccharomyces cerevisiae 851212 NP_009382.1 CDS ATS1 NC_001133.7 113615 114616 R Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle; Ats1p complement(113615..114616) Saccharomyces cerevisiae 851213 NP_009383.1 CDS FUN30 NC_001133.7 114920 118315 D Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fun30p 114920..118315 Saccharomyces cerevisiae 851214 NP_009384.1 CDS YAL018C NC_001133.7 118565 119542 R Yal018cp; Putative protein of unknown function complement(118565..119542) Saccharomyces cerevisiae 851215 NP_009385.1 CDS PSK1 NC_001133.7 120226 124296 D Psk1p; One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status 120226..124296 Saccharomyces cerevisiae 851216 NP_009386.1 CDS TPD3 NC_001133.7 124880 126787 D Tpd3p; Regulatory subunit A of the heterotrimeric protein phosphatase 2A, which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and for transcription by RNA polymerase III 124880..126787 Saccharomyces cerevisiae 851217 NP_009387.1 CDS NTG1 NC_001133.7 126904 128103 R Ntg1p; DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion complement(126904..128103) Saccharomyces cerevisiae 851218 NP_009388.2 CDS SYN8 NC_001133.7 128253 129020 R Syn8p; Endosomal SNARE related to mammalian syntaxin 8 complement(128253..129020) Saccharomyces cerevisiae 851219 NP_009389.2 CDS DEP1 NC_001133.7 129271 130533 D Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation; Dep1p 129271..130533 Saccharomyces cerevisiae 851220 NP_009390.1 CDS CYS3 NC_001133.7 130802 131986 D Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; Cys3p 130802..131986 Saccharomyces cerevisiae 851221 NP_009391.2 CDS SWC3 NC_001133.7 132202 134079 D Swc3p; Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae 132202..134079 Saccharomyces cerevisiae 851222 NP_009392.1 CDS MDM10 NC_001133.7 134186 135667 R Subunit of both the Mdm10-Mdm12-Mmm1 complex and the mitochondrial sorting and assembly machinery (SAM complex); functions in both the general and Tom40p-specific pathways for import and assembly of outer membrane beta-barrel proteins; Mdm10p complement(134186..135667) Saccharomyces cerevisiae 851223 NP_009393.1 CDS SPO7 NC_001133.7 135856 136635 D Spo7p; Regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic genes; required for normal nuclear envelope morphology and sporulation 135856..136635 Saccharomyces cerevisiae 851224 NP_009394.1 CDS FUN14 NC_001133.7 136916 137512 D Mitochondrial protein of unknown function; Fun14p 136916..137512 Saccharomyces cerevisiae 851225 NP_009395.1 CDS ERP2 NC_001133.7 137700 138347 R Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles; Erp2p complement(137700..138347) Saccharomyces cerevisiae 851226 NP_009396.2 CDS SSA1 NC_001133.7 139505 141433 R Ssa1p; ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall complement(139505..141433) Saccharomyces cerevisiae 851259 NP_009398.1 CDS EFB1 NC_001133.7 142176 143162 D Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site; Efb1p join(142176..142255,142622..143162) Saccharomyces cerevisiae 851260 NP_009399.2 CDS VPS8 NC_001133.7 143709 147533 D Vps8p; Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif 143709..147533 Saccharomyces cerevisiae 851261 NP_009400.1 CDS TFC3 NC_001133.7 147596 151168 R Tfc3p; Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding complement(join(147596..151008,151099..151168)) Saccharomyces cerevisiae 851262 NP_009401.1 CDS NUP60 NC_001133.7 152259 153878 D Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance; Nup60p 152259..153878 Saccharomyces cerevisiae 851263 NP_009402.1 CDS ERP1 NC_001133.7 154067 154726 R Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles; Erp1p complement(154067..154726) Saccharomyces cerevisiae 851264 NP_009403.1 CDS SWD1 NC_001133.7 155007 156287 D Swd1p; Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 155007..156287 Saccharomyces cerevisiae 851265 NP_009404.1 CDS RFA1 NC_001133.7 156756 158621 R Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; Rfa1p complement(156756..158621) Saccharomyces cerevisiae 851266 NP_009405.1 CDS SEN34 NC_001133.7 158967 159794 D Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease; Sen34p 158967..159794 Saccharomyces cerevisiae 851267 NP_009406.1 CDS YAR009C NC_001133.7 160598 164188 R Yar009cp; Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional complement(160598..164188) Saccharomyces cerevisiae 851268 NP_009407.1 CDS YAR010C NC_001133.7 164545 165867 R Yar010cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YARCTY1-1 TYB is mutant and probably non-functional complement(164545..165867) Saccharomyces cerevisiae 851269 NP_009408.2 CDS BUD14 NC_001133.7 166743 168866 R Protein involved in bud-site selection, Bud14p-Glc7p complex functions as a cortical regulator of dynein; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud14p complement(166743..168866) Saccharomyces cerevisiae 851271 NP_009409.1 CDS ADE1 NC_001133.7 169370 170290 D N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; Ade1p 169370..170290 Saccharomyces cerevisiae 851272 NP_009410.1 CDS KIN3 NC_001133.7 170391 171698 R Nonessential protein kinase with unknown cellular role; Kin3p complement(170391..171698) Saccharomyces cerevisiae 851273 NP_009411.1 CDS CDC15 NC_001133.7 172209 175133 R Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; Cdc15p complement(172209..175133) Saccharomyces cerevisiae 851274 NP_009412.1 CDS PAU7 NC_001133.7 176854 177021 R Pau7p; Part of 23-member seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme complement(176854..177021) Saccharomyces cerevisiae 851275 NP_009413.1 CDS YAR023C NC_001133.7 179279 179818 R Yar023cp; Putative integral membrane protein, member of DUP240 gene family complement(179279..179818) Saccharomyces cerevisiae 851276 NP_009414.1 CDS UIP3 NC_001133.7 183764 184471 D Uip3p; Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family 183764..184471 Saccharomyces cerevisiae 851279 NP_009415.1 CDS YAR028W NC_001133.7 184886 185590 D Yar028wp; Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS 184886..185590 Saccharomyces cerevisiae 851280 NP_009416.1 CDS YAR029W NC_001133.7 186315 186539 D Yar029wp; Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 186315..186539 Saccharomyces cerevisiae 851281 NP_009418.1 CDS PRM9 NC_001133.7 186830 187726 D Prm9p; Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family 186830..187726 Saccharomyces cerevisiae 851282 NP_009419.1 CDS MST28 NC_001133.7 188101 188805 D Putative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles; Mst28p 188101..188805 Saccharomyces cerevisiae 851284 NP_009420.1 CDS YAT1 NC_001133.7 190187 192250 D Yat1p; Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated 190187..192250 Saccharomyces cerevisiae 851285 NP_878042.1 CDS YAR035C-A NC_001133.7 192331 192411 R Yar035c-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(192331..192411) Saccharomyces cerevisiae 1466430 NP_009421.3 CDS SWH1 NC_001133.7 192613 196179 D Swh1p; Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction 192613..196179 Saccharomyces cerevisiae 851286 NP_009424.1 CDS FLO1 NC_001133.7 203394 208007 D Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p; Flo1p 203394..208007 Saccharomyces cerevisiae 851289 NP_009429.1 CDS YAR064W NC_001133.7 220189 220488 D Yar064wp; Putative protein of unknown function 220189..220488 Saccharomyces cerevisiae 851294 NP_009430.1 CDS YAR066W NC_001133.7 221040 221651 D Yar066wp; Putative GPI protein 221040..221651 Saccharomyces cerevisiae 851295 NP_009431.1 CDS YAR068W NC_001133.7 222397 222882 D Yar068wp; Fungal-specific protein of unknown function; induced in respiratory-deficient cells 222397..222882 Saccharomyces cerevisiae 851296 NP_009434.1 CDS PHO11 NC_001133.7 225451 226854 D Pho11p; One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 225451..226854 Saccharomyces cerevisiae 851299 NP_009437.1 CDS YBL113C NC_001134.7 280 2658 R Ybl113cp; Helicase-like protein encoded within the telomeric Y' element complement(280..2658) Saccharomyces cerevisiae 852159 NP_009438.1 CDS YBL112C NC_001134.7 2582 2899 R Ybl112cp; Putative protein of unknown function; YBL112C is contained within TEL02L complement(2582..2899) Saccharomyces cerevisiae 852160 NP_009439.1 CDS YBL111C NC_001134.7 2907 5009 R Ybl111cp; Helicase-like protein encoded within the telomeric Y' element complement(join(2907..4116,4216..5009)) Saccharomyces cerevisiae 852161 NP_076873.1 CDS PAU9 NC_001134.7 7605 7733 R hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.; Pau9p complement(7605..7733) Saccharomyces cerevisiae 852163 NP_009443.1 CDS YBL107C NC_001134.7 9961 10551 R Ybl107cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBL107C is not an essential gene complement(9961..10551) Saccharomyces cerevisiae 852167 NP_009444.1 CDS SRO77 NC_001134.7 10847 13879 R Sro77p; Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p complement(10847..13879) Saccharomyces cerevisiae 852168 NP_009445.1 CDS PKC1 NC_001134.7 14241 17696 R Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC); Pkc1p complement(14241..17696) Saccharomyces cerevisiae 852169 NP_009446.2 CDS YBL104C NC_001134.7 18177 21293 R Ybl104cp; Putative protein of unknown function, promoter contains multiple GCN4 binding sites complement(18177..21293) Saccharomyces cerevisiae 852170 NP_009447.1 CDS RTG3 NC_001134.7 22075 23535 R Rtg3p; Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways complement(22075..23535) Saccharomyces cerevisiae 852171 NP_009448.1 CDS SFT2 NC_001134.7 24098 24745 D Sft2p; Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 24098..24745 Saccharomyces cerevisiae 852172 NP_009449.1 CDS ECM21 NC_001134.7 24946 28299 R Non-essential protein of unknown function; promoter contains several Gcn4p binding elements; Ecm21p complement(24946..28299) Saccharomyces cerevisiae 852173 NP_878043.1 CDS YBL100W-C NC_001134.7 28427 28546 D Ybl100w-cp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 28427..28546 Saccharomyces cerevisiae 1466439 NP_009450.1 CDS YBL100W-B NC_001134.7 29932 35245 D Ybl100w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(29932..31224,31226..35245) Saccharomyces cerevisiae 852175 NP_009451.1 CDS YBL100W-A NC_001134.7 29932 31248 D Ybl100w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 29932..31248 Saccharomyces cerevisiae 852174 NP_009453.1 CDS ATP1 NC_001134.7 37050 38687 D Atp1p; Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated 37050..38687 Saccharomyces cerevisiae 852177 NP_009454.1 CDS BNA4 NC_001134.7 39142 40524 D Kynurenine 3-mono oxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; Bna4p 39142..40524 Saccharomyces cerevisiae 852179 NP_009455.2 CDS BRN1 NC_001134.7 40828 43092 D Essential protein required for chromosome condensation, likely to function as an intrinsic component of the condensation machinery, may influence multiple aspects of chromosome transmission and dynamics; Brn1p 40828..43092 Saccharomyces cerevisiae 852180 NP_009457.1 CDS YBL095W NC_001134.7 43274 44086 D Ybl095wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 43274..44086 Saccharomyces cerevisiae 852181 NP_009459.1 CDS ROX3 NC_001134.7 44253 44915 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; Rox3p complement(44253..44915) Saccharomyces cerevisiae 852184 NP_009460.1 CDS RPL32 NC_001134.7 45975 46367 D Rpl32p; Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencing 45975..46367 Saccharomyces cerevisiae 852185 NP_009461.2 CDS SCS22 NC_001134.7 46562 47177 R Scs22p; Protein involved in regulation of phospholipid metabolism; homolog of Scs2p; similar to D. melanogaster inturned protein complement(join(46562..47055,47144..47177)) Saccharomyces cerevisiae 852186 NP_009462.1 CDS MAP2 NC_001134.7 47360 48625 R Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p; Map2p complement(47360..48625) Saccharomyces cerevisiae 852187 NP_009463.1 CDS MRP21 NC_001134.7 48822 49355 D Mrp21p; Mitochondrial ribosomal protein of the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences 48822..49355 Saccharomyces cerevisiae 852188 NP_009464.2 CDS AVT5 NC_001134.7 49571 50950 D Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt5p 49571..50950 Saccharomyces cerevisiae 852189 NP_009465.1 CDS TEL1 NC_001134.7 51016 59379 R Tel1p; Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene complement(51016..59379) Saccharomyces cerevisiae 852190 NP_009466.1 CDS RPL23A NC_001134.7 59818 60735 R Rpl23ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli L14 and rat L23 ribosomal proteins complement(join(59818..60189,60694..60735)) Saccharomyces cerevisiae 852191 NP_009467.1 CDS YBL086C NC_001134.7 61199 62599 R Ybl086cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery complement(61199..62599) Saccharomyces cerevisiae 852192 NP_009468.1 CDS BOI1 NC_001134.7 63873 66815 D Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; Boi1p 63873..66815 Saccharomyces cerevisiae 852193 NP_009469.1 CDS CDC27 NC_001134.7 67166 69442 R Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Cdc27p complement(67166..69442) Saccharomyces cerevisiae 852194 NP_009471.1 CDS ALG3 NC_001134.7 69748 71124 R Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; Alg3p complement(69748..71124) Saccharomyces cerevisiae 852196 NP_009472.1 CDS YBL081W NC_001134.7 71863 72969 D Ybl081wp; Non-essential protein of unknown function 71863..72969 Saccharomyces cerevisiae 852197 NP_009473.1 CDS PET112 NC_001134.7 73067 74692 R Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog; Pet112p complement(73067..74692) Saccharomyces cerevisiae 852198 NP_009474.1 CDS NUP170 NC_001134.7 75256 79764 D Nup170p; Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p 75256..79764 Saccharomyces cerevisiae 852199 NP_009475.1 CDS ATG8 NC_001134.7 80375 80728 R Protein required for autophagy; modified by the serial action of Atg4p, Atg7p, and Atg3p, and conjugated at the C terminus with phosphatidylethanolamine, to become the form essential for generation of autophagosomes; Atg8p complement(80375..80728) Saccharomyces cerevisiae 852200 NP_009477.1 CDS ILS1 NC_001134.7 81041 84259 R Ils1p; Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A complement(81041..84259) Saccharomyces cerevisiae 852202 NP_009478.1 CDS SSA3 NC_001134.7 84497 86446 R Ssa3p; ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm complement(84497..86446) Saccharomyces cerevisiae 852203 NP_009479.1 CDS AAR2 NC_001134.7 86720 87787 R Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron; Aar2p complement(86720..87787) Saccharomyces cerevisiae 852205 NP_009481.1 CDS RPS8A NC_001134.7 88521 89123 R Rps8ap; Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein complement(88521..89123) Saccharomyces cerevisiae 852206 NP_878044.1 CDS YBL071C-B NC_001134.7 89456 89554 R Ybl071c-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(89456..89554) Saccharomyces cerevisiae 1466440 NP_660100.1 CDS KTI11 NC_001134.7 89976 90224 D Zn-ribbon electron carrier protein, required with Dph1p, Dph2p, Jjj3p, and Dph5p for synthesis of diphthamide, a modified histidine residue on Eft1p or Eft2p; required, with Elongator complex, for modification of wobble nucleosides in tRNA; Kti11p 89976..90224 Saccharomyces cerevisiae 852207 NP_009484.2 CDS AST1 NC_001134.7 90739 92028 D Peripheral membrane protein that interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane, possibly by influencing its incorporation into lipid rafts; Ast1p 90739..92028 Saccharomyces cerevisiae 852209 NP_009485.2 CDS PRS4 NC_001134.7 92412 93395 D Prs4p; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes 92412..93395 Saccharomyces cerevisiae 852211 NP_009486.2 CDS UBP13 NC_001134.7 93641 95884 R Ubp13p; Putative ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions complement(93641..95884) Saccharomyces cerevisiae 852212 NP_009487.2 CDS SEF1 NC_001134.7 96671 100117 R Sef1p; Putative transcription factor, has homolog in Kluyveromyces lactis complement(96671..100117) Saccharomyces cerevisiae 852214 NP_009489.1 CDS PRX1 NC_001134.7 100373 101158 R Prx1p; Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stress; phosphorylated complement(100373..101158) Saccharomyces cerevisiae 852215 NP_009490.1 CDS KIP1 NC_001134.7 101888 105223 D Kip1p; Kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation; functionally redundant with Cin8p 101888..105223 Saccharomyces cerevisiae 852216 NP_009492.1 CDS SKT5 NC_001134.7 105318 107408 R Skt5p; Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation complement(105318..107408) Saccharomyces cerevisiae 852218 NP_009493.1 CDS YEL1 NC_001134.7 107934 109997 D Yel1p; Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip 107934..109997 Saccharomyces cerevisiae 852219 NP_031358.1 CDS YBL059C-A NC_001134.7 110127 110541 R Ybl059c-ap; Putative protein of unknown function; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(join(110127..110422,110508..110541)) Saccharomyces cerevisiae 852220 NP_009494.1 CDS YBL059W NC_001134.7 110596 111246 D Ybl059wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies join(110596..110881,110951..111246) Saccharomyces cerevisiae 852221 NP_009495.1 CDS SHP1 NC_001134.7 111439 112710 D UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate; Shp1p 111439..112710 Saccharomyces cerevisiae 852222 NP_009496.1 CDS PTH2 NC_001134.7 112803 113447 R Pth2p; One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth complement(112803..113447) Saccharomyces cerevisiae 852223 NP_009497.1 CDS PTC3 NC_001134.7 113765 115171 D Type 2C protein phosphatase; dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA checkpoint inactivation; Ptc3p 113765..115171 Saccharomyces cerevisiae 852224 NP_009498.1 CDS YBL055C NC_001134.7 115576 116832 R Ybl055cp; 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases complement(115576..116832) Saccharomyces cerevisiae 852225 NP_009499.1 CDS YBL054W NC_001134.7 117592 119169 D Ybl054wp; Protein of unknown function involved in rRNA and ribosome biosynthesis 117592..119169 Saccharomyces cerevisiae 852226 NP_009501.1 CDS SAS3 NC_001134.7 119382 121877 R Sas3p; Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality complement(119382..121877) Saccharomyces cerevisiae 852228 NP_009502.1 CDS PIN4 NC_001134.7 122756 124762 R Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage; Pin4p complement(122756..124762) Saccharomyces cerevisiae 852229 NP_009503.1 CDS SEC17 NC_001134.7 125128 126122 D Sec17p; Peripheral membrane protein required for vesicular transport between ER and Golgi and for the 'priming' step in homotypic vacuole fusion, part of the cis-SNARE complex; has similarity to alpha-SNAP join(125128..125157,125274..126122) Saccharomyces cerevisiae 852230 NP_009504.1 CDS MOH1 NC_001134.7 126831 127247 D Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for viability in stationary phase; Moh1p 126831..127247 Saccharomyces cerevisiae 852231 NP_009506.1 CDS EDE1 NC_001134.7 127898 132043 R Key endocytic protein involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; Ede1p complement(127898..132043) Saccharomyces cerevisiae 852233 NP_009507.1 CDS PSY4 NC_001134.7 132427 133752 D Putative regulatory subunit of an evolutionarily conserved protein phosphatase complex containing the catalytic subunit Pph3p and a third subunit Psy2p; required for cisplatin resistance; GFP-fusion protein localizes to the nucleus; Psy4p 132427..133752 Saccharomyces cerevisiae 852234 NP_009508.1 CDS COR1 NC_001134.7 134146 135519 R Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain; Cor1p complement(134146..135519) Saccharomyces cerevisiae 852235 NP_009509.1 CDS YBL044W NC_001134.7 136001 136369 D Ybl044wp; Putative protein of unknown function; YBL044W is not an essential protein 136001..136369 Saccharomyces cerevisiae 852236 NP_009510.1 CDS ECM13 NC_001134.7 136691 137464 D Non-essential protein of unknown function; Ecm13p 136691..137464 Saccharomyces cerevisiae 852237 NP_009511.1 CDS FUI1 NC_001134.7 138344 140263 R High affinity uridine permease, localized to the plasma membrane; not involved in uracil transport; Fui1p complement(138344..140263) Saccharomyces cerevisiae 852238 NP_009512.1 CDS PRE7 NC_001134.7 141250 141975 D Pre7p; 20S proteasome beta-type subunit 141250..141975 Saccharomyces cerevisiae 852239 NP_009513.1 CDS ERD2 NC_001134.7 142115 142871 R HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins; Erd2p complement(join(142115..142752,142850..142871)) Saccharomyces cerevisiae 852240 NP_878045.1 CDS YBL039W-B NC_001134.7 143396 143575 D Ybl039w-bp; Putative protein of unknown function 143396..143575 Saccharomyces cerevisiae 1466441 NP_009514.1 CDS URA7 NC_001134.7 143992 145731 R Ura7p; Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis complement(143992..145731) Saccharomyces cerevisiae 852241 NP_009515.1 CDS MRPL16 NC_001134.7 146190 146888 D Mrpl16p; Mitochondrial ribosomal protein of the large subunit 146190..146888 Saccharomyces cerevisiae 852242 NP_009516.1 CDS APL3 NC_001134.7 147212 150289 D Apl3p; Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport 147212..150289 Saccharomyces cerevisiae 852243 NP_009517.1 CDS YBL036C NC_001134.7 150450 151223 R Ybl036cp; Single-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS complement(150450..151223) Saccharomyces cerevisiae 852244 NP_009518.1 CDS POL12 NC_001134.7 151496 153613 R Pol12p; B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation complement(151496..153613) Saccharomyces cerevisiae 852245 NP_009519.1 CDS STU1 NC_001134.7 153851 158392 R Stu1p; Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles complement(153851..158392) Saccharomyces cerevisiae 852246 NP_009520.1 CDS RIB1 NC_001134.7 158659 159696 R Rib1p; GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway complement(158659..159696) Saccharomyces cerevisiae 852247 NP_009521.1 CDS HEK2 NC_001134.7 160187 161332 D RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length; Hek2p 160187..161332 Saccharomyces cerevisiae 852248 NP_009522.1 CDS SHE1 NC_001134.7 161702 162718 D She1p; Cytoskeletal protein of unknown function; overexpression causes growth arrest 161702..162718 Saccharomyces cerevisiae 852249 NP_009523.1 CDS PET9 NC_001134.7 163044 164000 R Pet9p; Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; required for viability in many common lab strains carrying a mutation in the polymorphic SAL1 gene complement(163044..164000) Saccharomyces cerevisiae 852250 NP_076874.1 CDS YBL029C-A NC_001134.7 164491 164775 R Ybl029c-ap; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica complement(164491..164775) Saccharomyces cerevisiae 852251 NP_009524.1 CDS YBL029W NC_001134.7 166137 167267 D Ybl029wp; Non-essential protein of unknown function 166137..167267 Saccharomyces cerevisiae 852252 NP_009525.1 CDS YBL028C NC_001134.7 167521 167841 R Ybl028cp; Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis complement(167521..167841) Saccharomyces cerevisiae 852253 NP_009526.1 CDS RPL19B NC_001134.7 168426 169379 D Rpl19bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal join(168426..168427,168812..169379) Saccharomyces cerevisiae 852254 NP_009527.1 CDS LSM2 NC_001134.7 170626 171041 D Lsm2p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA join(170626..170679,170808..171041) Saccharomyces cerevisiae 852255 NP_009528.1 CDS RRN10 NC_001134.7 171484 171921 D Rrn10p; Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I 171484..171921 Saccharomyces cerevisiae 852256 NP_009529.1 CDS NCL1 NC_001134.7 172537 174591 D S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; similar to Nop2p and human proliferation associated nucleolar protein p120; Ncl1p 172537..174591 Saccharomyces cerevisiae 852257 NP_009530.1 CDS MCM2 NC_001134.7 174923 177529 R Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; Mcm2p complement(174923..177529) Saccharomyces cerevisiae 852258 NP_009531.1 CDS PIM1 NC_001134.7 177877 181278 R Pim1p; ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria complement(177877..181278) Saccharomyces cerevisiae 852259 NP_009532.1 CDS HAP3 NC_001134.7 181663 182097 R Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding; Hap3p complement(181663..182097) Saccharomyces cerevisiae 852260 NP_009533.1 CDS RFT1 NC_001134.7 182404 184128 D Rft1p; Flippase, essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane; mutation is suppressed by expression human p53 protein 182404..184128 Saccharomyces cerevisiae 852261 NP_009534.1 CDS APN2 NC_001134.7 184356 185918 D Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII; Apn2p 184356..185918 Saccharomyces cerevisiae 852262 NP_009535.1 CDS POP8 NC_001134.7 186001 186477 R Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Pop8p complement(join(186001..186355,186431..186477)) Saccharomyces cerevisiae 852263 NP_009536.1 CDS PEP1 NC_001134.7 186847 191586 R Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments; Pep1p complement(186847..191586) Saccharomyces cerevisiae 852264 NP_009537.1 CDS FUS3 NC_001134.7 192454 193515 D Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its degradation; Fus3p 192454..193515 Saccharomyces cerevisiae 852265 NP_009538.1 CDS ACH1 NC_001134.7 194125 195705 D Ach1p; Acetyl-coA hydrolase, primarily localized to mitochondria; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth 194125..195705 Saccharomyces cerevisiae 852266 NP_009539.1 CDS RRN6 NC_001134.7 199067 201751 R Rrn6p; Protein involved in the transcription of 35S rRNA genes by RNA polymerase I; component of the core factor (CF) complex also composed of Rrn11p, Rrn7p and TATA-binding protein complement(199067..201751) Saccharomyces cerevisiae 852269 NP_009540.2 CDS FMT1 NC_001134.7 202059 203264 D Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate; Fmt1p 202059..203264 Saccharomyces cerevisiae 852270 NP_009542.1 CDS SCT1 NC_001134.7 203541 205820 D Sct1p; Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed 203541..205820 Saccharomyces cerevisiae 852271 NP_009543.1 CDS YBL010C NC_001134.7 206110 206952 R Ybl010cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles complement(206110..206952) Saccharomyces cerevisiae 852273 NP_009544.1 CDS ALK2 NC_001134.7 207197 209227 D Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk1p and to mammalian haspins; Alk2p 207197..209227 Saccharomyces cerevisiae 852274 NP_878046.1 CDS YBL008W-A NC_001134.7 209412 209651 D Ybl008w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 209412..209651 Saccharomyces cerevisiae 1466442 NP_009545.1 CDS HIR1 NC_001134.7 209656 212178 D Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores; Hir1p 209656..212178 Saccharomyces cerevisiae 852275 NP_009546.1 CDS SLA1 NC_001134.7 212635 216369 R Sla1p; Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains complement(212635..216369) Saccharomyces cerevisiae 852276 NP_009547.2 CDS LDB7 NC_001134.7 216590 217132 R Ldb7p; Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions complement(216590..217132) Saccharomyces cerevisiae 852277 NP_009548.1 CDS PDR3 NC_001134.7 217473 220403 D Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); Pdr3p 217473..220403 Saccharomyces cerevisiae 852278 NP_009549.1 CDS YBL005W-B NC_001134.7 221333 226601 D Ybl005w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(221333..222637,222639..226601) Saccharomyces cerevisiae 852280 NP_009550.1 CDS YBL005W-A NC_001134.7 221333 222655 D Ybl005w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 221333..222655 Saccharomyces cerevisiae 852279 NP_009551.2 CDS UTP20 NC_001134.7 227639 235120 D Utp20p; Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA 227639..235120 Saccharomyces cerevisiae 852282 NP_009552.1 CDS HTA2 NC_001134.7 235397 235795 R One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; Hta2p complement(235397..235795) Saccharomyces cerevisiae 852283 NP_009553.1 CDS HTB2 NC_001134.7 236495 236890 D One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation; Htb2p 236495..236890 Saccharomyces cerevisiae 852284 NP_009554.1 CDS ECM15 NC_001134.7 237155 237469 R Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis; Ecm15p complement(237155..237469) Saccharomyces cerevisiae 852285 NP_009555.1 CDS NTH2 NC_001134.7 238943 241285 R Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses; Nth2p complement(238943..241285) Saccharomyces cerevisiae 852286 NP_009556.1 CDS RER2 NC_001134.7 241710 242570 R Rer2p; Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting complement(241710..242570) Saccharomyces cerevisiae 852287 NP_009557.1 CDS COQ1 NC_001134.7 242811 244232 D Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis; Coq1p 242811..244232 Saccharomyces cerevisiae 852288 NP_009558.1 CDS GPI18 NC_001134.7 244368 245669 R Gpi18p; Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria complement(244368..245669) Saccharomyces cerevisiae 852289 NP_009559.1 CDS RCR1 NC_001134.7 245908 246549 D Rcr1p; Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane 245908..246549 Saccharomyces cerevisiae 852290 NP_009560.1 CDS UGA2 NC_001134.7 247012 248505 D Uga2p; Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm 247012..248505 Saccharomyces cerevisiae 852291 NP_009561.1 CDS DSF2 NC_001134.7 248807 251017 R Dsf2p; Deletion suppressor of mpt5 mutation complement(248807..251017) Saccharomyces cerevisiae 852292 NP_009562.1 CDS FLR1 NC_001134.7 252564 254210 R Plasma membrane multidrug transporter of the major facilitator superfamily, involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; Flr1p complement(252564..254210) Saccharomyces cerevisiae 852293 NP_009563.1 CDS HHF1 NC_001134.7 255371 255682 R One of two identical histone H4 proteins (see also HHF2); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity; Hhf1p complement(255371..255682) Saccharomyces cerevisiae 852294 NP_009564.1 CDS HHT1 NC_001134.7 256329 256739 D One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation; Hht1p 256329..256739 Saccharomyces cerevisiae 852295 NP_009565.1 CDS IPP1 NC_001134.7 257110 257973 R Ipp1p; Cytoplasmic inorganic pyrophosphatase (PPase), catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase complement(257110..257973) Saccharomyces cerevisiae 852296 NP_009567.1 CDS YBR012W-B NC_001134.7 259867 265138 D Ybr012w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(259867..261171,261173..265138) Saccharomyces cerevisiae 852299 NP_009568.1 CDS YBR012W-A NC_001134.7 259867 261189 D Ybr012w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 259867..261189 Saccharomyces cerevisiae 852298 NP_009569.1 CDS YBR013C NC_001134.7 265490 265879 R Ybr013cp; Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein complement(265490..265879) Saccharomyces cerevisiae 852300 NP_009570.1 CDS GRX7 NC_001134.7 266725 267336 R Monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; membrane localized; forms homodimers; does not bind metal ions; Grx7p complement(266725..267336) Saccharomyces cerevisiae 852302 NP_009571.1 CDS MNN2 NC_001134.7 267710 269503 R Mnn2p; Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment complement(267710..269503) Saccharomyces cerevisiae 852303 NP_009572.1 CDS YBR016W NC_001134.7 270247 270633 D Ybr016wp; Plasma membrane protein of unknown function; has similarity to hydrophilins, which are hydrophilic, glycine-rich proteins involved in the adaptive response to hyperosmotic conditions 270247..270633 Saccharomyces cerevisiae 852304 NP_009573.1 CDS KAP104 NC_001134.7 270947 273703 R Transportin, cytosolic karyopherin beta 2 involved in delivery of heterogeneous nuclear ribonucleoproteins to the nucleoplasm, binds rg-nuclear localization signals on Nab2p and Hrp1p, plays a role in cell-cycle progression; Kap104p complement(270947..273703) Saccharomyces cerevisiae 852305 NP_009574.1 CDS GAL7 NC_001134.7 274427 275527 R Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism; Gal7p complement(274427..275527) Saccharomyces cerevisiae 852306 NP_009575.1 CDS GAL10 NC_001134.7 276253 278352 R UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers; Gal10p complement(276253..278352) Saccharomyces cerevisiae 852307 NP_009576.1 CDS GAL1 NC_001134.7 279021 280607 D Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; Gal1p 279021..280607 Saccharomyces cerevisiae 852308 NP_009577.1 CDS FUR4 NC_001134.7 281443 283344 D Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues; Fur4p 281443..283344 Saccharomyces cerevisiae 852309 NP_009578.1 CDS POA1 NC_001134.7 283738 284271 D Poa1p; Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing 283738..284271 Saccharomyces cerevisiae 852310 NP_009579.1 CDS CHS3 NC_001134.7 284428 287925 R Chs3p; Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan complement(284428..287925) Saccharomyces cerevisiae 852311 NP_009580.1 CDS SCO2 NC_001134.7 289445 290350 D Sco2p; Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p 289445..290350 Saccharomyces cerevisiae 852312 NP_009581.1 CDS OLA1 NC_001134.7 290681 291865 R P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein levels are induced by hydrogen peroxide; Ola1p complement(290681..291865) Saccharomyces cerevisiae 852313 NP_009582.1 CDS ETR1 NC_001134.7 292877 294019 R Etr1p; 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis complement(292877..294019) Saccharomyces cerevisiae 852314 NP_009584.1 CDS YBR028C NC_001134.7 294425 296002 R Ybr028cp; Putative protein kinase, possible substrate of cAMP-dependent protein kinase (PKA) complement(294425..296002) Saccharomyces cerevisiae 852316 NP_009585.1 CDS CDS1 NC_001134.7 296369 297742 R Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids; Cds1p complement(296369..297742) Saccharomyces cerevisiae 852317 NP_009586.1 CDS YBR030W NC_001134.7 298292 299950 D Ybr030wp; Putative protein of unknown function; predicted protein contains a SET domain (S-adenosyl-L-methionine-binding fold) 298292..299950 Saccharomyces cerevisiae 852318 NP_009587.1 CDS RPL4A NC_001134.7 300166 301254 D Rpl4ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins 300166..301254 Saccharomyces cerevisiae 852319 NP_009589.1 CDS EDS1 NC_001134.7 301944 304703 D Putative zinc cluster protein; YBR033W is not an essential gene; Eds1p 301944..304703 Saccharomyces cerevisiae 852321 NP_009590.1 CDS HMT1 NC_001134.7 304930 305976 R Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, thus facilitating nuclear export of these proteins; required for viability of npl3 mutants; Hmt1p complement(304930..305976) Saccharomyces cerevisiae 852322 NP_009591.1 CDS PDX3 NC_001134.7 306269 306955 R Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism; Pdx3p complement(306269..306955) Saccharomyces cerevisiae 852323 NP_009592.1 CDS CSG2 NC_001134.7 309081 310313 R Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; Csg2p complement(309081..310313) Saccharomyces cerevisiae 852324 NP_009593.1 CDS SCO1 NC_001134.7 310564 311451 R Sco1p; Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins complement(310564..311451) Saccharomyces cerevisiae 852325 NP_009594.1 CDS CHS2 NC_001134.7 311897 314788 D Chs2p; Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for the synthesis of chitin in the primary septum during cytokinesis 311897..314788 Saccharomyces cerevisiae 852326 NP_009595.1 CDS ATP3 NC_001134.7 315575 316510 D Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp3p 315575..316510 Saccharomyces cerevisiae 852327 NP_009596.1 CDS FIG1 NC_001134.7 316968 317864 D Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating; Fig1p 316968..317864 Saccharomyces cerevisiae 852328 NP_009597.2 CDS FAT1 NC_001134.7 318266 320275 D Fatty acid transporter and very long-chain fatty acyl-CoA synthetase, may form a complex with Faa1p or Faa4p that imports and activates exogenous fatty acids; Fat1p 318266..320275 Saccharomyces cerevisiae 852329 NP_009598.1 CDS CST26 NC_001134.7 320416 321609 R Protein of unknown function, affects chromosome stability when overexpressed; Cst26p complement(320416..321609) Saccharomyces cerevisiae 852330 NP_009599.1 CDS QDR3 NC_001134.7 321876 323945 R Qdr3p; Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin complement(321876..323945) Saccharomyces cerevisiae 852331 NP_009600.1 CDS TCM62 NC_001134.7 324337 326058 R Tcm62p; Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone complement(324337..326058) Saccharomyces cerevisiae 852332 NP_009601.1 CDS GIP1 NC_001134.7 328369 330090 R Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p; Gip1p complement(328369..330090) Saccharomyces cerevisiae 852334 NP_009602.1 CDS ZTA1 NC_001134.7 330505 331509 R Zta1p; Zeta-crystallin homolog, found in the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin, which has quinone oxidoreductase activity complement(330505..331509) Saccharomyces cerevisiae 852335 NP_009603.1 CDS FMP23 NC_001134.7 331831 332358 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp23p 331831..332358 Saccharomyces cerevisiae 852336 NP_009604.1 CDS RPS11B NC_001134.7 332829 333810 D Rps11bp; Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins join(332829..332873,333385..333810) Saccharomyces cerevisiae 852337 NP_009605.1 CDS REB1 NC_001134.7 334384 336816 R Reb1p; RNA polymerase I enhancer binding protein; DNA binding protein which binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription complement(334384..336816) Saccharomyces cerevisiae 852338 NP_009606.1 CDS REG2 NC_001134.7 337181 338197 R Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; Reg2p complement(337181..338197) Saccharomyces cerevisiae 852340 NP_009608.1 CDS RFS1 NC_001134.7 338718 339350 R Rfs1p; Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(338718..339350) Saccharomyces cerevisiae 852341 NP_009609.1 CDS YBR053C NC_001134.7 339673 340749 R Ybr053cp; Putative protein of unknown function; induced by cell wall perturbation complement(339673..340749) Saccharomyces cerevisiae 852342 NP_009610.1 CDS YRO2 NC_001134.7 343099 344133 D Yro2p; Putative protein of unknown function; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p 343099..344133 Saccharomyces cerevisiae 852343 NP_009611.1 CDS PRP6 NC_001134.7 344600 347299 R Splicing factor, component of the U4/U6-U5 snRNP complex; Prp6p complement(344600..347299) Saccharomyces cerevisiae 852344 NP_009612.1 CDS YBR056W NC_001134.7 347877 349382 D Ybr056wp; Putative cytoplasmic protein of unknown function 347877..349382 Saccharomyces cerevisiae 852346 NP_009613.1 CDS MUM2 NC_001134.7 352191 353291 R Mum2p; Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex complement(352191..353291) Saccharomyces cerevisiae 852348 NP_009614.2 CDS UBP14 NC_001134.7 353670 356015 R Ubp14p; Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T complement(353670..356015) Saccharomyces cerevisiae 852349 NP_116327.1 CDS TSC3 NC_001134.7 356322 356564 R Tsc3p; Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis complement(356322..356564) Saccharomyces cerevisiae 852350 NP_009615.1 CDS AKL1 NC_001134.7 356859 360185 R Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization; Akl1p complement(356859..360185) Saccharomyces cerevisiae 852351 NP_009616.1 CDS ORC2 NC_001134.7 360650 362512 R Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; Orc2p complement(360650..362512) Saccharomyces cerevisiae 852352 NP_009617.1 CDS TRM7 NC_001134.7 364785 365717 R Trm7p; 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop complement(364785..365717) Saccharomyces cerevisiae 852353 NP_009618.2 CDS YBR062C NC_001134.7 365974 366598 R Ybr062cp complement(join(365974..366500,366583..366598)) Saccharomyces cerevisiae 852354 NP_009619.1 CDS YBR063C NC_001134.7 366968 368182 R Ybr063cp; Putative protein of unknown function; YBR063C is not an essential gene complement(366968..368182) Saccharomyces cerevisiae 852356 NP_009621.1 CDS ECM2 NC_001134.7 368582 369676 R Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p; Ecm2p complement(368582..369676) Saccharomyces cerevisiae 852357 NP_009622.1 CDS NRG2 NC_001134.7 370035 370697 R Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p; Nrg2p complement(370035..370697) Saccharomyces cerevisiae 852358 NP_009623.1 CDS TIP1 NC_001134.7 372100 372732 R Tip1p; Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins complement(372100..372732) Saccharomyces cerevisiae 852359 NP_009624.1 CDS BAP2 NC_001134.7 373858 375687 R High-affinity leucine permease, functions as a branched-chain amino acid permease involved in the uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; Bap2p complement(373858..375687) Saccharomyces cerevisiae 852360 NP_009625.1 CDS TAT1 NC_001134.7 376571 378430 R Tat1p; Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance complement(376571..378430) Saccharomyces cerevisiae 852361 NP_009626.1 CDS ALG14 NC_001134.7 379218 379931 R Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases; Alg14p complement(379218..379931) Saccharomyces cerevisiae 852362 NP_009627.1 CDS YBR071W NC_001134.7 380408 381043 D Ybr071wp; Putative protein of unknown function; (GFP)-fusion and epitope-tagged proteins localize to the cytoplasm; mRNA expression may be regulated by the cell cycle and/or cell wall stress 380408..381043 Saccharomyces cerevisiae 852363 NP_009628.1 CDS HSP26 NC_001134.7 382027 382671 D Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires a heat-induced conformational change; not expressed in unstressed cells; Hsp26p 382027..382671 Saccharomyces cerevisiae 852364 NP_878048.1 CDS YBR072C-A NC_001134.7 382855 383016 R Ybr072c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(382855..383016) Saccharomyces cerevisiae 1466444 NP_009629.2 CDS RDH54 NC_001134.7 383209 385983 D Rdh54p; DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference 383209..385983 Saccharomyces cerevisiae 852365 NP_009630.2 CDS YBR074W NC_001134.7 386281 389211 D Ybr074wp; Putative metalloprotease 386281..389211 Saccharomyces cerevisiae 852366 NP_009632.1 CDS ECM8 NC_001134.7 390369 391388 D Non-essential protein of unknown function; Ecm8p 390369..391388 Saccharomyces cerevisiae 852368 NP_009633.1 CDS SLM4 NC_001134.7 391799 392287 R Slm4p; Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 complement(391799..392287) Saccharomyces cerevisiae 852369 NP_009634.1 CDS ECM33 NC_001134.7 393118 394854 D GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p; Ecm33p join(393118..393175,393506..394854) Saccharomyces cerevisiae 852370 NP_009635.1 CDS RPG1 NC_001134.7 395377 398271 R Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; Rpg1p complement(395377..398271) Saccharomyces cerevisiae 852371 NP_009636.1 CDS SEC18 NC_001134.7 398608 400884 R Sec18p; ATPase required for the release of Sec17p during the 'priming' step in homotypic vacuole fusion and for ER to Golgi transport; homolog of the mammalian NSF complement(398608..400884) Saccharomyces cerevisiae 852372 NP_009637.1 CDS SPT7 NC_001134.7 401247 405245 R Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex; Spt7p complement(401247..405245) Saccharomyces cerevisiae 852373 NP_009638.1 CDS UBC4 NC_001134.7 406622 407163 R Ubiquitin-conjugating enzyme (E2), mediates degradation of short-lived and abnormal proteins; interacts with E3-CaM in ubiquitinating calmodulin; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; Ubc4p complement(join(406622..407021,407117..407163)) Saccharomyces cerevisiae 852376 NP_009639.1 CDS TEC1 NC_001134.7 409163 410623 D Transcription factor required for full Ty1 epxression, Ty1-mediated gene activation, and haploid invasive and diploid pseudohyphal growth; TEA/ATTS DNA-binding domain family member; Tec1p 409163..410623 Saccharomyces cerevisiae 852377 NP_009640.1 CDS MIS1 NC_001134.7 411048 413975 D Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase; Mis1p 411048..413975 Saccharomyces cerevisiae 852378 NP_009641.1 CDS RPL19A NC_001134.7 414180 415255 R Rpl19ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal complement(join(414180..414747,415254..415255)) Saccharomyces cerevisiae 852379 NP_009642.1 CDS AAC3 NC_001134.7 415977 416900 D Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration; Aac3p 415977..416900 Saccharomyces cerevisiae 852380 NP_061491.1 CDS YBR085C-A NC_001134.7 418901 419158 R Ybr085c-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus complement(418901..419158) Saccharomyces cerevisiae 852381 NP_009643.2 CDS IST2 NC_001134.7 420195 423035 R Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process; Ist2p complement(420195..423035) Saccharomyces cerevisiae 852382 NP_009644.1 CDS RFC5 NC_001134.7 423759 424823 D Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc5p 423759..424823 Saccharomyces cerevisiae 852383 NP_009645.1 CDS POL30 NC_001134.7 424984 425760 R Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; Pol30p complement(424984..425760) Saccharomyces cerevisiae 852385 NP_009647.1 CDS NHP6B NC_001134.7 426184 426483 R Nhp6bp; High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters complement(426184..426483) Saccharomyces cerevisiae 852386 NP_009648.1 CDS YBR090C NC_001134.7 426327 427052 R Ybr090cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(join(426327..426510,426868..427052)) Saccharomyces cerevisiae 852387 NP_009649.1 CDS MRS5 NC_001134.7 427149 427478 R Mrs5p; Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins complement(427149..427478) Saccharomyces cerevisiae 852388 NP_009650.1 CDS PHO3 NC_001134.7 427692 429095 R Pho3p; Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin complement(427692..429095) Saccharomyces cerevisiae 852389 NP_009651.1 CDS PHO5 NC_001134.7 429542 430945 R Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2; Pho5p complement(429542..430945) Saccharomyces cerevisiae 852390 NP_009652.1 CDS PBY1 NC_001134.7 432030 434291 D Putative tubulin tyrosine ligase associated with P-bodies; Pby1p 432030..434291 Saccharomyces cerevisiae 852391 NP_009653.2 CDS RXT2 NC_001134.7 434400 435692 R Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; Rxt2p complement(434400..435692) Saccharomyces cerevisiae 852392 NP_009654.1 CDS YBR096W NC_001134.7 436015 436707 D Ybr096wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER 436015..436707 Saccharomyces cerevisiae 852393 NP_009655.1 CDS VPS15 NC_001134.7 436945 441309 D Vps15p; Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p 436945..441309 Saccharomyces cerevisiae 852394 NP_009656.2 CDS MMS4 NC_001134.7 441509 443584 D Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair; Mms4p 441509..443584 Saccharomyces cerevisiae 852395 NP_009659.1 CDS FES1 NC_001134.7 443815 444687 R Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; Fes1p complement(443815..444687) Saccharomyces cerevisiae 852397 NP_009660.1 CDS EXO84 NC_001134.7 445056 447317 R Exo84p; Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis complement(445056..447317) Saccharomyces cerevisiae 852398 NP_009661.1 CDS SIF2 NC_001134.7 447703 449310 D WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus; Sif2p 447703..449310 Saccharomyces cerevisiae 852399 NP_009662.1 CDS YMC2 NC_001134.7 449661 450650 D Ymc2p; Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc1p 449661..450650 Saccharomyces cerevisiae 852401 NP_009663.1 CDS VID24 NC_001134.7 450875 451963 R Vid24p; Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; involved in proteasome-dependent catabolite degradation of FBPase complement(450875..451963) Saccharomyces cerevisiae 852402 NP_009664.1 CDS PHO88 NC_001134.7 452652 453218 D Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations; Pho88p 452652..453218 Saccharomyces cerevisiae 852403 NP_009665.1 CDS IML3 NC_001134.7 453787 454524 R Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p; Iml3p complement(453787..454524) Saccharomyces cerevisiae 852404 NP_009666.2 CDS AIM3 NC_001134.7 454816 457659 D Protein interacting with Rsv167p; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim3p 454816..457659 Saccharomyces cerevisiae 852405 NP_009667.1 CDS CMD1 NC_001134.7 457913 458356 R Cmd1p; Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin complement(457913..458356) Saccharomyces cerevisiae 852406 NP_009668.1 CDS ALG1 NC_001134.7 458866 460215 D Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog; Alg1p 458866..460215 Saccharomyces cerevisiae 852407 NP_009669.1 CDS YSA1 NC_001134.7 461172 461867 R Ysa1p; Nudix hydrolase family member with ADP-ribose pyrophosphatase activity complement(461172..461867) Saccharomyces cerevisiae 852408 NP_878049.2 CDS SUS1 NC_001134.7 462133 462573 D Sus1p; Protein involved in mRNA export coupled transcription activation; component of the SAGA histone acetylase complex join(462133..462203,462284..462423,462494..462573) Saccharomyces cerevisiae 1466445 NP_009670.1 CDS CYC8 NC_001134.7 462864 465764 R General transcriptional co-repressor, acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; Cyc8p complement(462864..465764) Saccharomyces cerevisiae 852410 NP_009672.1 CDS RAD16 NC_001134.7 467242 469614 D Rad16p; Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex 467242..469614 Saccharomyces cerevisiae 852411 NP_009673.1 CDS LYS2 NC_001134.7 469742 473920 R Lys2p; Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p complement(469742..473920) Saccharomyces cerevisiae 852412 NP_009675.1 CDS TKL2 NC_001134.7 474386 476431 R Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; Tkl2p complement(474386..476431) Saccharomyces cerevisiae 852414 NP_009676.1 CDS TEF2 NC_001134.7 477665 479041 D Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; Tef2p 477665..479041 Saccharomyces cerevisiae 852415 NP_009677.1 CDS MUD1 NC_001134.7 479332 480317 D U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing; Mud1p join(479332..479339,479429..480317) Saccharomyces cerevisiae 852416 NP_009678.1 CDS CBP6 NC_001134.7 480429 480917 R Mitochondrial translational activator of the COB mRNA; phosphorylated; Cbp6p complement(480429..480917) Saccharomyces cerevisiae 852417 NP_009679.1 CDS GRS1 NC_001134.7 481358 483361 R Grs1p; Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation complement(481358..483361) Saccharomyces cerevisiae 852418 NP_009680.2 CDS MRPL36 NC_001134.7 483964 484497 R Mrpl36p; Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region complement(483964..484497) Saccharomyces cerevisiae 852419 NP_009681.1 CDS TFC1 NC_001134.7 484736 486685 R Tfc1p; One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 complement(484736..486685) Saccharomyces cerevisiae 852421 NP_009683.1 CDS PTC4 NC_001134.7 487193 488374 R Ptc4p; Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity complement(487193..488374) Saccharomyces cerevisiae 852422 NP_009684.1 CDS TPS1 NC_001134.7 488899 490386 R Tps1p; Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway complement(488899..490386) Saccharomyces cerevisiae 852423 NP_009685.1 CDS VMA2 NC_001134.7 491263 492816 R Vma2p; Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm complement(491263..492816) Saccharomyces cerevisiae 852424 NP_009686.1 CDS ATG14 NC_001134.7 493075 494109 R Subunit of an autophagy-specific phosphatidylinositol 3-kinase complex (with Vps34p, Vps15p, and Vps30p) required for organization of a pre-autophagosomal structure; ATG14 transcription is activated by Gln3p during nitrogen starvation; Atg14p complement(493075..494109) Saccharomyces cerevisiae 852425 NP_009687.1 CDS OPY1 NC_001134.7 494347 495333 R Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Opy1p complement(494347..495333) Saccharomyces cerevisiae 852426 NP_009688.1 CDS SHE3 NC_001134.7 495586 496863 R She3p; Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance complement(495586..496863) Saccharomyces cerevisiae 852427 NP_009689.1 CDS CCZ1 NC_001134.7 497157 499271 D Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway; Ccz1p 497157..499271 Saccharomyces cerevisiae 852428 NP_009690.1 CDS AGP2 NC_001134.7 499646 501436 R High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease; Agp2p complement(499646..501436) Saccharomyces cerevisiae 852429 NP_009691.1 CDS HSL7 NC_001134.7 501798 504281 R Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; Hsl7p complement(501798..504281) Saccharomyces cerevisiae 852431 NP_009693.1 CDS CKS1 NC_001134.7 504848 505300 D Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; Cks1p 504848..505300 Saccharomyces cerevisiae 852432 NP_009694.1 CDS MEC1 NC_001134.7 505662 512768 D Mec1p; Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination 505662..512768 Saccharomyces cerevisiae 852433 NP_009695.1 CDS YBR137W NC_001134.7 513038 513577 D Ybr137wp; Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); YBR137W is not an essential gene 513038..513577 Saccharomyces cerevisiae 852434 NP_009696.1 CDS YBR138C NC_001134.7 513756 515330 R Ybr138cp; Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene complement(513756..515330) Saccharomyces cerevisiae 852435 NP_009697.1 CDS YBR139W NC_001134.7 515658 517184 D Ybr139wp; Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner 515658..517184 Saccharomyces cerevisiae 852436 NP_009698.1 CDS IRA1 NC_001134.7 517344 526622 R Ira1p; GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase complement(517344..526622) Saccharomyces cerevisiae 852437 NP_009699.1 CDS YBR141C NC_001134.7 527019 528032 R Ybr141cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene complement(527019..528032) Saccharomyces cerevisiae 852438 NP_009700.1 CDS MAK5 NC_001134.7 528311 530632 D Mak5p; Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits 528311..530632 Saccharomyces cerevisiae 852439 NP_009701.1 CDS SUP45 NC_001134.7 530863 532176 R Sup45p; Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor complement(530863..532176) Saccharomyces cerevisiae 852440 NP_009703.1 CDS ADH5 NC_001134.7 533756 534811 D Alcohol dehydrogenase isoenzyme V; involved in ethanol production; Adh5p 533756..534811 Saccharomyces cerevisiae 852442 NP_009704.1 CDS MRPS9 NC_001134.7 535254 536090 D Mrps9p; Mitochondrial ribosomal protein of the small subunit 535254..536090 Saccharomyces cerevisiae 852443 NP_009705.1 CDS YBR147W NC_001134.7 536569 537459 D Ybr147wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays fluconazole resistance 536569..537459 Saccharomyces cerevisiae 852444 NP_009706.1 CDS YSW1 NC_001134.7 537870 539699 D Ysw1p; Protein expressed specifically in spores 537870..539699 Saccharomyces cerevisiae 852445 NP_009707.1 CDS ARA1 NC_001134.7 539981 541015 D NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product; Ara1p 539981..541015 Saccharomyces cerevisiae 852446 NP_009708.1 CDS TBS1 NC_001134.7 541203 544487 R Tbs1p; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(541203..544487) Saccharomyces cerevisiae 852447 NP_009709.1 CDS APD1 NC_001134.7 545022 545972 D Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus; Apd1p 545022..545972 Saccharomyces cerevisiae 852448 NP_009710.1 CDS SPP381 NC_001134.7 546370 547245 D mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; Spp381p 546370..547245 Saccharomyces cerevisiae 852449 NP_009711.1 CDS RIB7 NC_001134.7 547454 548188 D Rib7p; Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway 547454..548188 Saccharomyces cerevisiae 852450 NP_009712.1 CDS RPB5 NC_001134.7 548356 549003 R Rpb5p; RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation complement(548356..549003) Saccharomyces cerevisiae 852451 NP_009713.1 CDS CNS1 NC_001134.7 549765 550922 D TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion; Cns1p 549765..550922 Saccharomyces cerevisiae 852452 NP_009714.1 CDS SLI15 NC_001134.7 551098 553194 R Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase; Sli15p complement(551098..553194) Saccharomyces cerevisiae 852453 NP_009715.2 CDS ICS2 NC_001134.7 553537 554304 R Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization; Ics2p complement(553537..554304) Saccharomyces cerevisiae 852454 NP_009716.1 CDS AMN1 NC_001134.7 556543 558192 D Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN); Amn1p 556543..558192 Saccharomyces cerevisiae 852455 NP_009717.1 CDS IFA38 NC_001134.7 558679 559722 D Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides; Ifa38p 558679..559722 Saccharomyces cerevisiae 852456 NP_009718.1 CDS CDC28 NC_001134.7 560072 560968 D Cdc28p; Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates 560072..560968 Saccharomyces cerevisiae 852457 NP_009719.1 CDS CSH1 NC_001134.7 561629 562759 D Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; Csh1p 561629..562759 Saccharomyces cerevisiae 852458 NP_009720.1 CDS TOS1 NC_001134.7 563198 564565 R Tos1p; Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C complement(563198..564565) Saccharomyces cerevisiae 852459 NP_009721.1 CDS YSY6 NC_001134.7 565226 565423 D Ysy6p; Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion 565226..565423 Saccharomyces cerevisiae 852460 NP_009722.1 CDS DEM1 NC_001134.7 565718 567475 D Protein of unknown function, shows similarity to RNA-processing protein Pta1p; Dem1p 565718..567475 Saccharomyces cerevisiae 852461 NP_009723.1 CDS ARL1 NC_001134.7 567870 568421 R Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor; Arl1p complement(567870..568421) Saccharomyces cerevisiae 852462 NP_009724.1 CDS UBS1 NC_001134.7 568847 569680 D Ubs1p; Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity 568847..569680 Saccharomyces cerevisiae 852463 NP_009725.1 CDS TYR1 NC_001134.7 569837 571195 R Tyr1p; Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels complement(569837..571195) Saccharomyces cerevisiae 852464 NP_009726.1 CDS POP7 NC_001134.7 571463 571885 R Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Pop7p complement(571463..571885) Saccharomyces cerevisiae 852465 NP_009727.1 CDS PEX32 NC_001134.7 572366 573607 D Pex32p; Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p 572366..573607 Saccharomyces cerevisiae 852466 NP_009728.1 CDS SSE2 NC_001134.7 573910 575991 R Sse2p; Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p complement(573910..575991) Saccharomyces cerevisiae 852467 NP_009729.1 CDS NPL4 NC_001134.7 576339 578081 R Npl4p; Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation complement(576339..578081) Saccharomyces cerevisiae 852468 NP_009730.1 CDS SEC66 NC_001134.7 578359 578979 D Sec66p; Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER 578359..578979 Saccharomyces cerevisiae 852469 NP_009731.2 CDS SMY2 NC_001134.7 579145 581367 R Smy2p; Protein of unknown function that interacts with Myo2p; has similarity to S. pombe Mpd2 complement(579145..581367) Saccharomyces cerevisiae 852470 NP_009732.1 CDS UMP1 NC_001134.7 581721 582167 R Ump1p; Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly complement(581721..582167) Saccharomyces cerevisiae 852471 NP_009734.1 CDS SWD3 NC_001134.7 582403 583350 D Swd3p; Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 582403..583350 Saccharomyces cerevisiae 852472 NP_009735.1 CDS ECM31 NC_001134.7 583715 584653 D Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate; Ecm31p 583715..584653 Saccharomyces cerevisiae 852474 NP_009736.1 CDS EHT1 NC_001134.7 584802 586157 R Eht1p; Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; contains esterase activity; localizes to lipid particles and the mitochondrial outer membrane complement(584802..586157) Saccharomyces cerevisiae 852476 NP_009738.1 CDS FZO1 NC_001134.7 586542 589109 R Mitochondrial integral membrane protein involved in mitochondrial fusion and maintenance of the mitochondrial genome; contains N-terminal GTPase domain; Fzo1p complement(586542..589109) Saccharomyces cerevisiae 852477 NP_009739.1 CDS DTR1 NC_001134.7 589736 591454 D Putative dityrosine transporter, required for spore wall synthesis; expressed during sporulation; member of the major facilitator superfamily (DHA1 family) of multidrug resistance transporters; Dtr1p 589736..591454 Saccharomyces cerevisiae 852478 NP_009740.1 CDS RPS6B NC_001134.7 591707 592769 R Rps6bp; Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein complement(join(591707..592411,592764..592769)) Saccharomyces cerevisiae 852479 NP_009741.1 CDS SMP1 NC_001134.7 593501 594859 R Smp1p; Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors complement(593501..594859) Saccharomyces cerevisiae 852480 NP_878050.1 CDS YBR182C-A NC_001134.7 595356 595550 R Ybr182c-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(595356..595550) Saccharomyces cerevisiae 1466446 NP_009742.1 CDS YPC1 NC_001134.7 596110 597060 D Ypc1p; Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance 596110..597060 Saccharomyces cerevisiae 852481 NP_009743.1 CDS YBR184W NC_001134.7 597358 598929 D Ybr184wp; Putative protein of unknown function; YBR184W is not an essential gene 597358..598929 Saccharomyces cerevisiae 852482 NP_009744.1 CDS MBA1 NC_001134.7 599118 599954 R Protein involved in assembly of mitochondrial respiratory complexes; may act as a receptor for proteins destined for export from the mitochondrial matrix to the inner membrane; Mba1p complement(599118..599954) Saccharomyces cerevisiae 852483 NP_009745.2 CDS PCH2 NC_001134.7 600548 602355 D Pch2p; Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA join(600548..602098,602212..602355) Saccharomyces cerevisiae 852484 NP_009746.1 CDS GDT1 NC_001134.7 602629 603471 D Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; Gdt1p 602629..603471 Saccharomyces cerevisiae 852485 NP_009747.1 CDS NTC20 NC_001134.7 603680 604102 R Ntc20p; Member of a complex, including Prp19p, that binds to the spliceosome; required for pre-mRNA splicing complement(603680..604102) Saccharomyces cerevisiae 852486 NP_009748.1 CDS RPS9B NC_001134.7 604503 605503 D Rps9bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins join(604503..604509,604923..605503) Saccharomyces cerevisiae 852487 NP_009750.1 CDS RPL21A NC_001134.7 606265 607135 D Rpl21ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein join(606265..606275,606664..607135) Saccharomyces cerevisiae 852489 NP_009751.1 CDS RIM2 NC_001134.7 607647 608780 D Rim2p; Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family 607647..608780 Saccharomyces cerevisiae 852491 NP_009752.1 CDS MED8 NC_001134.7 609077 609748 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med8p complement(609077..609748) Saccharomyces cerevisiae 852492 NP_009753.1 CDS AIM4 NC_001134.7 610033 610404 D Protein proposed to be associated with the nuclear pore complex; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and a severe growth defect in minimal glycerol media; Aim4p 610033..610404 Saccharomyces cerevisiae 852493 NP_009754.1 CDS MSI1 NC_001134.7 610609 611877 R Subunit of chromatin assembly factor I (CAF-1), negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase; localizes to the nucleus and cytoplasm; homologous to human retinoblastoma binding proteins RbAp48 and RbAp46; Msi1p complement(610609..611877) Saccharomyces cerevisiae 852494 NP_009755.1 CDS PGI1 NC_001134.7 612231 613895 R Pgi1p; Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation complement(612231..613895) Saccharomyces cerevisiae 852495 NP_878051.1 CDS YBR196C-A NC_001134.7 614019 614168 R Ybr196c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(614019..614168) Saccharomyces cerevisiae 1466447 NP_878052.1 CDS YBR196C-B NC_001134.7 614521 614625 R Ybr196c-bp; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(614521..614625) Saccharomyces cerevisiae 1466448 NP_009756.1 CDS YBR197C NC_001134.7 615198 615851 R Ybr197cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene complement(615198..615851) Saccharomyces cerevisiae 852496 NP_009757.1 CDS TAF5 NC_001134.7 616122 618518 R Taf5p; Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification complement(616122..618518) Saccharomyces cerevisiae 852497 NP_009758.1 CDS KTR4 NC_001134.7 618904 620298 D Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Ktr4p 618904..620298 Saccharomyces cerevisiae 852498 NP_009759.1 CDS BEM1 NC_001134.7 620867 622522 D Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p; Bem1p 620867..622522 Saccharomyces cerevisiae 852499 NP_878053.1 CDS YBR200W-A NC_001134.7 622978 623142 D Ybr200w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 622978..623142 Saccharomyces cerevisiae 1466449 NP_009760.1 CDS DER1 NC_001134.7 623572 624207 D Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p; Der1p 623572..624207 Saccharomyces cerevisiae 852500 NP_001032571.1 CDS YBR201C-A NC_001134.7 624489 624692 R Ybr201c-ap; Putative protein of unknown function complement(624489..624692) Saccharomyces cerevisiae 3799967 NP_009761.1 CDS MCM7 NC_001134.7 625767 628304 D Mcm7p; Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase 625767..628304 Saccharomyces cerevisiae 852501 NP_009762.1 CDS COS111 NC_001134.7 629163 631937 D Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Cos111p 629163..631937 Saccharomyces cerevisiae 852502 NP_009763.1 CDS YBR204C NC_001134.7 632249 633376 R Ybr204cp; Serine hydrolase; YBR204C is not an essential gene complement(632249..633376) Saccharomyces cerevisiae 852503 NP_009764.1 CDS KTR3 NC_001134.7 633617 634831 D Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Ktr3p 633617..634831 Saccharomyces cerevisiae 852504 NP_009766.1 CDS FTH1 NC_001134.7 635141 636538 D Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; Fth1p 635141..636538 Saccharomyces cerevisiae 852506 NP_009767.1 CDS DUR1,2 NC_001134.7 636698 642205 R Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; Dur1,2p complement(636698..642205) Saccharomyces cerevisiae 852507 NP_009769.1 CDS ERV15 NC_001134.7 645545 645973 D Protein involved in export of proteins from the endoplasmic reticulum, has similarity to Erv14p; Erv15p 645545..645973 Saccharomyces cerevisiae 852511 NP_009770.1 CDS AME1 NC_001134.7 646153 647127 R Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; Ame1p complement(646153..647127) Saccharomyces cerevisiae 852512 NP_009771.1 CDS NGR1 NC_001134.7 647881 649899 D RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; expressed in stationary phase; Ngr1p 647881..649899 Saccharomyces cerevisiae 852513 NP_009772.1 CDS MET8 NC_001134.7 650363 651187 D Met8p; Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis 650363..651187 Saccharomyces cerevisiae 852514 NP_009773.1 CDS SDS24 NC_001134.7 651410 652993 D Sds24p; One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis 651410..652993 Saccharomyces cerevisiae 852515 NP_009774.2 CDS HPC2 NC_001134.7 653351 655312 D Hpc2p; Highly charged, basic protein required for normal cell-cycle regulation of histone gene transcription; mutants display strong synthetic defects with subunits of FACT, a complex that allows RNA Pol II to elongate through nucleosomes join(653351..653363,653448..655312) Saccharomyces cerevisiae 852516 NP_009775.1 CDS YBP1 NC_001134.7 655571 657595 R Ybp1p; Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress complement(655571..657595) Saccharomyces cerevisiae 852517 NP_009776.1 CDS ATG12 NC_001134.7 657827 658387 D Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy; Atg12p 657827..658387 Saccharomyces cerevisiae 852518 NP_009777.1 CDS PYC2 NC_001134.7 658702 662244 R Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis; Pyc2p complement(658702..662244) Saccharomyces cerevisiae 852519 NP_009778.1 CDS YBR219C NC_001134.7 662494 663298 R Ybr219cp; Putative protein of unknown function; YBR219C is not an essential gene complement(join(662494..662576,662998..663298)) Saccharomyces cerevisiae 852520 NP_009779.1 CDS YBR220C NC_001134.7 662990 664672 R Ybr220cp; Putative protein of unknown function; YBR220C is not an essential gene complement(662990..664672) Saccharomyces cerevisiae 852521 NP_009780.1 CDS PDB1 NC_001134.7 665148 666248 R Pdb1p; E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria complement(665148..666248) Saccharomyces cerevisiae 852522 NP_878054.1 CDS YBR221W-A NC_001134.7 666533 666637 D Ybr221w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 666533..666637 Saccharomyces cerevisiae 1466450 NP_009781.1 CDS PCS60 NC_001134.7 666715 668346 R Peroxisomal AMP-binding protein, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase; Pcs60p complement(666715..668346) Saccharomyces cerevisiae 852523 NP_009782.1 CDS TDP1 NC_001134.7 668658 670292 R Tyrosyl-DNA Phosphodiesterase I, hydrolyzes 3'-phosphotyrosyl bonds to generate 3'-phosphate DNA and tyrosine, involved in the repair of DNA lesions created by topoisomerase I; Tdp1p complement(668658..670292) Saccharomyces cerevisiae 852525 NP_009784.1 CDS YBR225W NC_001134.7 670622 673324 D Ybr225wp; Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components 670622..673324 Saccharomyces cerevisiae 852526 NP_009786.1 CDS MCX1 NC_001134.7 673567 675129 R Mitochondrial ATP-binding protein, possibly a mitochondrial chaperone with non-proteolytic function; similar to bacterial ClpX proteins; Mcx1p complement(673567..675129) Saccharomyces cerevisiae 852528 NP_009787.1 CDS SLX1 NC_001134.7 675308 676222 D Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p; Slx1p 675308..676222 Saccharomyces cerevisiae 852529 NP_009788.1 CDS ROT2 NC_001134.7 676352 679216 R Rot2p; Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations complement(676352..679216) Saccharomyces cerevisiae 852530 NP_009789.1 CDS OM14 NC_001134.7 679544 680045 R Om14p; Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron complement(join(679544..679937,680035..680045)) Saccharomyces cerevisiae 852531 NP_001018029.1 CDS YBR230W-A NC_001134.7 680357 680557 D Ybr230w-ap; Putative protein of unknown function 680357..680557 Saccharomyces cerevisiae 2777172 NP_009790.1 CDS SWC5 NC_001134.7 682174 683085 R Swc5p; Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A complement(682174..683085) Saccharomyces cerevisiae 852532 NP_009792.1 CDS PBP2 NC_001134.7 683423 684664 D RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; Pbp2p 683423..684664 Saccharomyces cerevisiae 852533 NP_631876.1 CDS DAD3 NC_001134.7 684972 685256 D Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Dad3p 684972..685256 Saccharomyces cerevisiae 852535 NP_009793.1 CDS ARC40 NC_001134.7 685433 686587 R Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; Arc40p complement(685433..686587) Saccharomyces cerevisiae 852536 NP_009794.1 CDS YBR235W NC_001134.7 686896 690258 D Ybr235wp; Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene 686896..690258 Saccharomyces cerevisiae 852537 NP_009795.1 CDS ABD1 NC_001134.7 690378 691688 R Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; Abd1p complement(690378..691688) Saccharomyces cerevisiae 852538 NP_009796.1 CDS PRP5 NC_001134.7 691964 694513 D RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA; Prp5p 691964..694513 Saccharomyces cerevisiae 852539 NP_009797.1 CDS YBR238C NC_001134.7 695102 697297 R Ybr238cp; Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span complement(695102..697297) Saccharomyces cerevisiae 852540 NP_009798.1 CDS YBR239C NC_001134.7 698349 699938 R Ybr239cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene complement(698349..699938) Saccharomyces cerevisiae 852541 NP_009799.1 CDS THI2 NC_001134.7 700485 701837 R Zinc finger protein of the Zn(II)2Cys6 type, probable transcriptional activator of thiamine biosynthetic genes; Thi2p complement(700485..701837) Saccharomyces cerevisiae 852542 NP_009800.1 CDS YBR241C NC_001134.7 702584 704050 R Ybr241cp; Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene complement(702584..704050) Saccharomyces cerevisiae 852543 NP_009801.1 CDS YBR242W NC_001134.7 704665 705381 D Ybr242wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene 704665..705381 Saccharomyces cerevisiae 852544 NP_009802.1 CDS ALG7 NC_001134.7 705442 706788 R UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin; Alg7p complement(705442..706788) Saccharomyces cerevisiae 852545 NP_009803.1 CDS GPX2 NC_001134.7 707523 708011 D Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; Gpx2p 707523..708011 Saccharomyces cerevisiae 852546 NP_009804.1 CDS ISW1 NC_001134.7 708145 711534 R Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation; Isw1p complement(708145..711534) Saccharomyces cerevisiae 852547 NP_009805.1 CDS YBR246W NC_001134.7 711586 712749 D Ybr246wp; Putative protein of unknown function; deletion results in a weak carboxypeptidase Y missorting/secretion phenotype; YBR246W is not an essential gene 711586..712749 Saccharomyces cerevisiae 852548 NP_009806.1 CDS ENP1 NC_001134.7 712999 714450 R Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; Enp1p complement(712999..714450) Saccharomyces cerevisiae 852549 NP_009807.1 CDS HIS7 NC_001134.7 714802 716460 R Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor; His7p complement(714802..716460) Saccharomyces cerevisiae 852550 NP_009808.1 CDS ARO4 NC_001134.7 716877 717989 R Aro4p; 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan complement(716877..717989) Saccharomyces cerevisiae 852551 NP_009809.1 CDS SPO23 NC_001134.7 719028 720599 D Spo23p; Protein of unknown function; associates with meiosis-specific protein Spo1p 719028..720599 Saccharomyces cerevisiae 852552 NP_009810.1 CDS MRPS5 NC_001134.7 721385 722308 D Mrps5p; Mitochondrial ribosomal protein of the small subunit 721385..722308 Saccharomyces cerevisiae 852553 NP_009811.1 CDS DUT1 NC_001134.7 722606 723049 D dUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; Dut1p 722606..723049 Saccharomyces cerevisiae 852554 NP_009812.1 CDS SRB6 NC_001134.7 723265 723630 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Srb6p 723265..723630 Saccharomyces cerevisiae 852555 NP_009813.1 CDS TRS20 NC_001134.7 723731 724258 R Trs20p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder complement(723731..724258) Saccharomyces cerevisiae 852556 NP_009814.1 CDS YBR255W NC_001134.7 724451 726535 D Ybr255wp; Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 724451..726535 Saccharomyces cerevisiae 852557 NP_116328.2 CDS YBR255C-A NC_001134.7 726613 727069 R Ybr255c-ap; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species complement(join(726613..726912,727007..727069)) Saccharomyces cerevisiae 852558 NP_009815.1 CDS RIB5 NC_001134.7 727381 728097 R Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway; Rib5p complement(727381..728097) Saccharomyces cerevisiae 852559 NP_009816.1 CDS POP4 NC_001134.7 728880 729719 D Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P; Pop4p 728880..729719 Saccharomyces cerevisiae 852560 NP_009817.1 CDS SHG1 NC_001134.7 729729 730157 R Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; Shg1p complement(729729..730157) Saccharomyces cerevisiae 852561 NP_009818.1 CDS YBR259W NC_001134.7 730382 732448 D Ybr259wp; Putative protein of unknown function; YBR259W is not an essential gene 730382..732448 Saccharomyces cerevisiae 852562 NP_009819.1 CDS RGD1 NC_001134.7 732634 734634 R Rgd1p; GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization complement(732634..734634) Saccharomyces cerevisiae 852563 NP_009820.1 CDS YBR261C NC_001134.7 734827 735525 R Ybr261cp; Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR261C is not an essential gene complement(734827..735525) Saccharomyces cerevisiae 852564 NP_009821.1 CDS AIM5 NC_001134.7 735715 736035 R Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss and reduced growth rate in minimal glycerol media; Aim5p complement(735715..736035) Saccharomyces cerevisiae 852566 NP_009822.2 CDS SHM1 NC_001134.7 736259 737731 D Shm1p; Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine 736259..737731 Saccharomyces cerevisiae 852565 NP_009823.2 CDS YPT10 NC_001134.7 737765 738364 R Ypt10p; GTP binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles complement(737765..738364) Saccharomyces cerevisiae 852567 NP_009824.1 CDS TSC10 NC_001134.7 738577 739539 D Tsc10p; 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family 738577..739539 Saccharomyces cerevisiae 852568 NP_009825.1 CDS REI1 NC_001134.7 739836 741017 D Rei1p; Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network 739836..741017 Saccharomyces cerevisiae 852569 NP_009827.1 CDS MRPL37 NC_001134.7 741294 741611 D Mrpl37p; Mitochondrial ribosomal protein of the large subunit 741294..741611 Saccharomyces cerevisiae 852571 NP_009828.2 CDS FMP21 NC_001134.7 742155 742571 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp21p complement(742155..742571) Saccharomyces cerevisiae 852572 NP_009829.1 CDS BIT2 NC_001134.7 742756 744393 R Bit2p complement(742756..744393) Saccharomyces cerevisiae 852573 NP_009830.1 CDS YBR271W NC_001134.7 744847 746106 D Ybr271wp; Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR271W is not as essential gene 744847..746106 Saccharomyces cerevisiae 852574 NP_009831.1 CDS HSM3 NC_001134.7 746356 747798 R Protein of unknown function, involved in DNA mismatch repair during slow growth; has weak similarity to Msh1p; Hsm3p complement(746356..747798) Saccharomyces cerevisiae 852575 NP_009832.1 CDS UBX7 NC_001134.7 748056 749366 R Ubx7p; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p complement(748056..749366) Saccharomyces cerevisiae 852576 NP_009833.1 CDS CHK1 NC_001134.7 749589 751172 D Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase; Chk1p 749589..751172 Saccharomyces cerevisiae 852577 NP_009834.1 CDS RIF1 NC_001134.7 751351 757101 R Rif1p; Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation complement(751351..757101) Saccharomyces cerevisiae 852578 NP_009835.1 CDS PPS1 NC_001134.7 757616 760039 R Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle; Pps1p complement(757616..760039) Saccharomyces cerevisiae 852579 NP_009837.1 CDS DPB3 NC_001134.7 760290 760895 D Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle; Dpb3p 760290..760895 Saccharomyces cerevisiae 852580 NP_009838.1 CDS PAF1 NC_001134.7 761253 762590 D RNAP II-associated protein; defines large complex biochemically and functionally distinct from the Srb-Mediator form of Pol II holoenzyme; required for full expression of a subset of cell cycle-regulated genes; homolog of human PD2/hPAF1; Paf1p 761253..762590 Saccharomyces cerevisiae 852582 NP_009839.2 CDS SAF1 NC_001134.7 762780 764693 R Saf1p; F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 complement(762780..764693) Saccharomyces cerevisiae 852583 NP_009840.1 CDS DUG2 NC_001134.7 764966 767602 R Probable di- and tri-peptidase; forms a complex with Dug1p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); Dug2p complement(764966..767602) Saccharomyces cerevisiae 852584 NP_009841.1 CDS MRPL27 NC_001134.7 768236 768676 D Mrpl27p; Mitochondrial ribosomal protein of the large subunit 768236..768676 Saccharomyces cerevisiae 852585 NP_009842.1 CDS SSH1 NC_001134.7 768939 770411 R Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential; Ssh1p complement(768939..770411) Saccharomyces cerevisiae 852586 NP_009843.1 CDS YBR284W NC_001134.7 771235 773628 D Ybr284wp; Putative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin 771235..773628 Saccharomyces cerevisiae 852587 NP_009844.1 CDS YBR285W NC_001134.7 773918 774352 D Ybr285wp; Putative protein of unknown function; YBR285W is not an essential gene and deletion of YBR285W leads to poor growth on glucose-minimal medium at 15C 773918..774352 Saccharomyces cerevisiae 852588 NP_009845.2 CDS APE3 NC_001134.7 774696 776309 D Vacuolar aminopeptidase Y, processed to mature form by Prb1p; Ape3p 774696..776309 Saccharomyces cerevisiae 852589 NP_009846.1 CDS YBR287W NC_001134.7 776567 777850 D Ybr287wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene 776567..777850 Saccharomyces cerevisiae 852590 NP_009847.1 CDS APM3 NC_001134.7 778008 779459 R Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; Apm3p complement(778008..779459) Saccharomyces cerevisiae 852591 NP_009848.1 CDS SNF5 NC_001134.7 779663 782380 D Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; Snf5p 779663..782380 Saccharomyces cerevisiae 852592 NP_009849.1 CDS BSD2 NC_001134.7 782587 783552 D Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification; Bsd2p 782587..783552 Saccharomyces cerevisiae 852593 NP_009850.1 CDS CTP1 NC_001134.7 783669 784568 R Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family; Ctp1p complement(783669..784568) Saccharomyces cerevisiae 852594 NP_009852.1 CDS VBA2 NC_001134.7 787001 788425 D Vba2p; Permease of basic amino acids in the vacuolar membrane 787001..788425 Saccharomyces cerevisiae 852596 NP_009853.1 CDS SUL1 NC_001134.7 789230 791809 D Sul1p; High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates 789230..791809 Saccharomyces cerevisiae 852597 NP_009854.1 CDS PCA1 NC_001134.7 792843 796493 D Pca1p; Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; S288C and most other lab strains contain a G970R mutation which eliminates normal cadmium transport function 792843..796493 Saccharomyces cerevisiae 852598 NP_009855.1 CDS PHO89 NC_001134.7 796792 798516 R Pho89p; Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p complement(796792..798516) Saccharomyces cerevisiae 852599 NP_878055.1 CDS YBR296C-A NC_001134.7 800117 800236 R Ybr296c-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(800117..800236) Saccharomyces cerevisiae 1466451 NP_009856.1 CDS MAL33 NC_001134.7 800517 801923 D Mal33p; MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C 800517..801923 Saccharomyces cerevisiae 852600 NP_009857.1 CDS MAL31 NC_001134.7 802625 804469 R Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C; Mal31p complement(802625..804469) Saccharomyces cerevisiae 852601 NP_878056.1 CDS YBR298C-A NC_001134.7 805029 805250 R Ybr298c-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(805029..805250) Saccharomyces cerevisiae 1466452 NP_009858.1 CDS MAL32 NC_001134.7 805345 807099 D Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; Mal32p 805345..807099 Saccharomyces cerevisiae 852602 NP_009860.1 CDS DAN3 NC_001134.7 809051 809413 D Dan3p; Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth 809051..809413 Saccharomyces cerevisiae 852603 NP_009861.1 CDS COS2 NC_001134.7 810334 811473 R Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos2p complement(810334..811473) Saccharomyces cerevisiae 852605 NP_009863.2 CDS YCL073C NC_001135.4 6479 8326 R Ycl073cp; Protein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YKR106W complement(6479..8326) Saccharomyces cerevisiae 850289 NP_009864.1 CDS VBA3 NC_001135.4 9706 11082 D Vba3p; Permease of basic amino acids in the vacuolar membrane 9706..11082 Saccharomyces cerevisiae 850290 NP_009865.2 CDS YCL068C NC_001135.4 11503 12285 R Ycl068cp; Putative protein of unknown function complement(11503..12285) Saccharomyces cerevisiae 850291 NP_009866.1 CDS HMLALPHA2 NC_001135.4 12386 13018 R Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression; Hmlalpha2p complement(12386..13018) Saccharomyces cerevisiae 850292 NP_009867.1 CDS HMLALPHA1 NC_001135.4 13282 13809 D Silenced copy of ALPHA1 at HML, encoding a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression; Hmlalpha1p 13282..13809 Saccharomyces cerevisiae 850293 NP_001018030.1 CDS CHA1 NC_001135.4 15798 16880 R Cha1p; Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine complement(15798..16880) Saccharomyces cerevisiae 850295 NP_009870.1 CDS VAC17 NC_001135.4 17290 18561 D Vac17p; Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p 17290..18561 Saccharomyces cerevisiae 850296 NP_009871.2 CDS MRC1 NC_001135.4 18816 22106 R S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; Mrc1p complement(18816..22106) Saccharomyces cerevisiae 850297 NP_009872.1 CDS KRR1 NC_001135.4 22429 23379 R Krr1p; Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit complement(22429..23379) Saccharomyces cerevisiae 850298 NP_001018031.2 CDS YCL058W-A NC_001135.4 23584 23925 D Ycl058w-ap; Protein of unknown function; identified by homology to Ashbya gossypii 23584..23925 Saccharomyces cerevisiae 3289591 NP_065435.1 CDS YCL057C-A NC_001135.4 24032 24325 R Ycl057c-ap; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(24032..24325) Saccharomyces cerevisiae 850300 NP_009874.1 CDS PRD1 NC_001135.4 24768 26906 D Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; Prd1p 24768..26906 Saccharomyces cerevisiae 850301 NP_009875.1 CDS YCL056C NC_001135.4 26925 27359 R Ycl056cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(26925..27359) Saccharomyces cerevisiae 850302 NP_009876.1 CDS KAR4 NC_001135.4 27929 28936 D Transcription factor required for gene regulation in repsonse to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone; Kar4p 27929..28936 Saccharomyces cerevisiae 850303 NP_009877.1 CDS SPB1 NC_001135.4 31449 33974 D Spb1p; AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants 31449..33974 Saccharomyces cerevisiae 850304 NP_009878.1 CDS PBN1 NC_001135.4 34143 35393 R Pbn1p; Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X complement(34143..35393) Saccharomyces cerevisiae 850305 NP_009879.2 CDS LRE1 NC_001135.4 35865 37616 D Protein involved in control of cell wall structure and stress response; inhibits Cbk1p protein kinase activity; overproduction confers resistance to cell-wall degrading enzymes; Lre1p 35865..37616 Saccharomyces cerevisiae 850306 NP_009880.2 CDS APA1 NC_001135.4 37836 38801 R Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p; Apa1p complement(37836..38801) Saccharomyces cerevisiae 850307 NP_009881.2 CDS YCL049C NC_001135.4 39786 40724 R Ycl049cp; Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene complement(39786..40724) Saccharomyces cerevisiae 850308 NP_001032573.1 CDS YCL048W-A NC_001135.4 41488 41727 D Ycl048w-ap; Putative protein of unknown function 41488..41727 Saccharomyces cerevisiae 3799968 NP_009882.1 CDS SPS22 NC_001135.4 42165 43556 D Sps22p; Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall 42165..43556 Saccharomyces cerevisiae 850309 NP_009883.1 CDS YCL047C NC_001135.4 43661 44437 R Ycl047cp; Putative protein of unknown function complement(43661..44437) Saccharomyces cerevisiae 850310 NP_009884.1 CDS YCL045C NC_001135.4 44623 46905 R Ycl045cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts with Gal80p; YCL045C is not an essential gene complement(44623..46905) Saccharomyces cerevisiae 850312 NP_009886.1 CDS MGR1 NC_001135.4 47111 48364 R Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; required for growth of cells lacking the mitochondrial genome; Mgr1p complement(47111..48364) Saccharomyces cerevisiae 850313 NP_009887.1 CDS PDI1 NC_001135.4 48653 50221 R Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; Pdi1p complement(48653..50221) Saccharomyces cerevisiae 850314 NP_009889.2 CDS YCL042W NC_001135.4 50584 50943 D Ycl042wp; Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm 50584..50943 Saccharomyces cerevisiae 850315 NP_009890.1 CDS GLK1 NC_001135.4 50838 52340 D Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; Glk1p 50838..52340 Saccharomyces cerevisiae 850317 NP_009891.1 CDS GID7 NC_001135.4 52645 54882 D Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions; Gid7p 52645..54882 Saccharomyces cerevisiae 850318 NP_009892.1 CDS ATG22 NC_001135.4 54941 56527 R Protein required for the breakdown of autophagic vesicles in the vacuole during autophagy, putative integral membrane protein that localizes to vacuolar membranes and punctate structures attached to the vacuole; Atg22p complement(54941..56527) Saccharomyces cerevisiae 850319 NP_009893.2 CDS SRO9 NC_001135.4 57374 58678 R Sro9p; Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif complement(57374..58678) Saccharomyces cerevisiae 850320 NP_009894.1 CDS GFD2 NC_001135.4 59026 60726 D Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation; Gfd2p 59026..60726 Saccharomyces cerevisiae 850321 NP_009895.1 CDS GRX1 NC_001135.4 60841 61173 R Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage; Grx1p complement(60841..61173) Saccharomyces cerevisiae 850322 NP_009896.2 CDS LSB5 NC_001135.4 61658 62722 D Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; Lsb5p 61658..62722 Saccharomyces cerevisiae 850323 NP_009897.1 CDS YCL033C NC_001135.4 62776 63282 R Ycl033cp; Putative protein-methionine-R-oxide reductase; involved in response to oxidative stress; similar to mouse Sepx1p and fly SelRp; YCL033C is not an essential gene complement(62776..63282) Saccharomyces cerevisiae 850324 NP_009898.1 CDS STE50 NC_001135.4 63441 64481 D Ste50p; Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction 63441..64481 Saccharomyces cerevisiae 850325 NP_009899.1 CDS RRP7 NC_001135.4 64675 65568 R Rrp7p; Essential protein involved in rRNA processing and ribosome biogenesis complement(64675..65568) Saccharomyces cerevisiae 850326 NP_009900.2 CDS HIS4 NC_001135.4 65934 68333 R Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis; His4p complement(65934..68333) Saccharomyces cerevisiae 850327 NP_009901.1 CDS BIK1 NC_001135.4 68599 69921 R Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170; Bik1p complement(68599..69921) Saccharomyces cerevisiae 850328 NP_009902.2 CDS RNQ1 NC_001135.4 70150 71367 D [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate; Rnq1p 70150..71367 Saccharomyces cerevisiae 850329 NP_009903.2 CDS FUS1 NC_001135.4 71803 73341 D Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate; Fus1p 71803..73341 Saccharomyces cerevisiae 850330 NP_065436.1 CDS HBN1 NC_001135.4 73405 73986 R Putative protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; Hbn1p complement(73405..73986) Saccharomyces cerevisiae 850331 NP_009904.2 CDS FRM2 NC_001135.4 74704 75285 R Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis; similar to bacterial nitroreductases; Frm2p complement(74704..75285) Saccharomyces cerevisiae 850332 NP_009905.3 CDS AGP1 NC_001135.4 76018 77919 R Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); Agp1p complement(76018..77919) Saccharomyces cerevisiae 850333 NP_009907.2 CDS KCC4 NC_001135.4 79162 82275 D Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; Kcc4p 79162..82275 Saccharomyces cerevisiae 850334 NP_065437.1 CDS YCL021W-A NC_001135.4 83620 83997 D Ycl021w-ap; Putative protein of unknown function 83620..83997 Saccharomyces cerevisiae 850338 NP_009909.2 CDS YCL019W NC_001135.4 85102 90415 D Ycl019wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(85102..86391,86393..90415) Saccharomyces cerevisiae 850340 NP_009910.2 CDS YCL020W NC_001135.4 85102 86418 D Ycl020wp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 85102..86418 Saccharomyces cerevisiae 850339 NP_009911.2 CDS LEU2 NC_001135.4 91324 92418 D Leu2p; Beta-isopropylmalate dehydrogenase (IMDH), catalyzes the third step in the leucine biosynthesis pathway 91324..92418 Saccharomyces cerevisiae 850342 NP_009912.2 CDS NFS1 NC_001135.4 92777 94270 R Nfs1p; Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria complement(92777..94270) Saccharomyces cerevisiae 850343 NP_009913.2 CDS DCC1 NC_001135.4 94621 95763 R Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance; Dcc1p complement(94621..95763) Saccharomyces cerevisiae 850344 NP_009914.2 CDS BUD3 NC_001135.4 96281 101191 D Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; Bud3p 96281..101191 Saccharomyces cerevisiae 850345 NP_976246.1 CDS YCL012C NC_001135.4 101317 101788 R Ycl012cp; Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene complement(join(101317..101633,101701..101788)) Saccharomyces cerevisiae 2746859 NP_009916.1 CDS GBP2 NC_001135.4 102075 103358 R Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro; Gbp2p complement(102075..103358) Saccharomyces cerevisiae 850346 NP_009917.1 CDS SGF29 NC_001135.4 103571 104350 R Sgf29p; SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex complement(103571..104350) Saccharomyces cerevisiae 850347 NP_009918.1 CDS ILV6 NC_001135.4 104619 105548 R Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria; Ilv6p complement(104619..105548) Saccharomyces cerevisiae 850348 NP_009919.3 CDS STP22 NC_001135.4 105696 106853 R Stp22p; Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype complement(105696..106853) Saccharomyces cerevisiae 850349 NP_958835.1 CDS VMA9 NC_001135.4 107023 107417 D Vma9p; Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis join(107023..107033,107111..107191,107288..107417) Saccharomyces cerevisiae 2732686 NP_009922.2 CDS LDB16 NC_001135.4 108021 108791 D Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria; Ldb16p 108021..108791 Saccharomyces cerevisiae 850351 NP_009923.2 CDS PGS1 NC_001135.4 109105 110670 D Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis; Pgs1p 109105..110670 Saccharomyces cerevisiae 850352 NP_009924.3 CDS YCL002C NC_001135.4 110843 111674 R Ycl002cp; Putative protein of unknown function; YCL002C is not an essential gene complement(join(110843..111556,111633..111674)) Saccharomyces cerevisiae 850353 NP_009925.1 CDS RER1 NC_001135.4 111914 112480 D Rer1p; Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER 111914..112480 Saccharomyces cerevisiae 850354 NP_009926.1 CDS YCL001W-A NC_001135.4 113078 113539 D Ycl001w-ap; Putative protein of unknown function; YCL001W-A is not an essential gene 113078..113539 Saccharomyces cerevisiae 850355 NP_076878.1 CDS YCL001W-B NC_001135.4 113768 114022 D Ycl001w-bp; Putative protein of unknown function; identified by homology 113768..114022 Saccharomyces cerevisiae 850356 NP_009928.1 CDS CDC10 NC_001135.4 117378 118346 R Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM; Cdc10p complement(117378..118346) Saccharomyces cerevisiae 850358 NP_009929.1 CDS MRPL32 NC_001135.4 118618 119169 D Mrpl32p; Mitochondrial ribosomal protein of the large subunit 118618..119169 Saccharomyces cerevisiae 850359 NP_009930.1 CDS YCP4 NC_001135.4 119573 120316 R Ycp4p; Protein of unknown function, has sequence and structural similarity to flavodoxins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(119573..120316) Saccharomyces cerevisiae 850360 NP_009931.1 CDS CIT2 NC_001135.4 120944 122326 R Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; Cit2p complement(120944..122326) Saccharomyces cerevisiae 850361 NP_009933.1 CDS YCR007C NC_001135.4 126009 126728 R Ycr007cp; Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene complement(126009..126728) Saccharomyces cerevisiae 850364 NP_009934.1 CDS SAT4 NC_001135.4 128468 130279 D Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p; Sat4p 128468..130279 Saccharomyces cerevisiae 850366 NP_009935.1 CDS RVS161 NC_001135.4 130743 131540 R Rvs161p; Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress complement(130743..131540) Saccharomyces cerevisiae 850367 NP_009936.1 CDS ADY2 NC_001135.4 132271 133122 R Ady2p; Acetate transporter required for normal sporulation; phosphorylated in mitochondria complement(132271..133122) Saccharomyces cerevisiae 850368 NP_009937.2 CDS ADP1 NC_001135.4 133722 136871 R Putative ATP-dependent permease of the ABC transporter family of proteins; Adp1p complement(133722..136871) Saccharomyces cerevisiae 850369 NP_009938.2 CDS PGK1 NC_001135.4 137744 138994 D Pgk1p; 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis 137744..138994 Saccharomyces cerevisiae 850370 NP_009940.2 CDS POL4 NC_001135.4 139183 140931 R Pol4p; DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta complement(139183..140931) Saccharomyces cerevisiae 850372 NP_009941.2 CDS YCR015C NC_001135.4 141215 142168 R Ycr015cp; Putative protein of unknown function; YCR015C is not an essential gene complement(141215..142168) Saccharomyces cerevisiae 850373 NP_009942.2 CDS YCR016W NC_001135.4 143632 144504 D Ycr016wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; YCR016W is not an essential gene 143632..144504 Saccharomyces cerevisiae 850375 NP_009943.2 CDS CWH43 NC_001135.4 144771 147632 R Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion; Cwh43p complement(144771..147632) Saccharomyces cerevisiae 850376 NP_009944.2 CDS SRD1 NC_001135.4 148236 148901 R Srd1p; Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation complement(148236..148901) Saccharomyces cerevisiae 850377 NP_009946.1 CDS MAK32 NC_001135.4 152835 153926 D Protein necessary for structural stability of L-A double-stranded RNA-containing particles; Mak32p 152835..153926 Saccharomyces cerevisiae 850381 NP_009947.1 CDS PET18 NC_001135.4 154010 154657 R Protein required for respiratory growth and stability of the mitochondrial genome; Pet18p complement(154010..154657) Saccharomyces cerevisiae 850382 NP_009948.1 CDS MAK31 NC_001135.4 154828 155094 R Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family; Mak31p complement(154828..155094) Saccharomyces cerevisiae 850383 NP_009949.1 CDS HTL1 NC_001135.4 155318 155554 D Htl1p; Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance 155318..155554 Saccharomyces cerevisiae 850384 NP_009950.1 CDS HSP30 NC_001135.4 156105 157103 R Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase; Hsp30p complement(156105..157103) Saccharomyces cerevisiae 850385 NP_009952.2 CDS YCR023C NC_001135.4 158534 160369 R Ycr023cp; Putative protein of unknown function; YCR023C is not an essential gene complement(158534..160369) Saccharomyces cerevisiae 850387 NP_009953.1 CDS SLM5 NC_001135.4 160740 162218 R Slm5p; Mitochondrial asparaginyl-tRNA synthetase complement(160740..162218) Saccharomyces cerevisiae 850388 NP_878057.1 CDS YCR024C-B NC_001135.4 162595 162861 R Ycr024c-bp; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(162595..162861) Saccharomyces cerevisiae 1466398 NP_058137.1 CDS PMP1 NC_001135.4 162941 163063 R Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; Pmp1p complement(162941..163063) Saccharomyces cerevisiae 850389 NP_009955.2 CDS NPP1 NC_001135.4 164107 166335 R Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; Npp1p complement(164107..166335) Saccharomyces cerevisiae 850391 NP_009956.2 CDS RHB1 NC_001135.4 167366 167995 R Rhb1p; Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins complement(167366..167995) Saccharomyces cerevisiae 850392 NP_009957.1 CDS FEN2 NC_001135.4 170882 172420 R Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; Fen2p complement(170882..172420) Saccharomyces cerevisiae 850394 NP_009958.2 CDS RIM1 NC_001135.4 172946 173436 R Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; Rim1p complement(join(172946..173111,173195..173436)) Saccharomyces cerevisiae 850395 NP_009959.2 CDS SYP1 NC_001135.4 173822 176434 R Syp1p; Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation complement(173822..176434) Saccharomyces cerevisiae 850396 NP_009960.2 CDS RPS14A NC_001135.4 177496 178216 R Rps14ap; Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins complement(join(177496..177902,178210..178216)) Saccharomyces cerevisiae 850397 NP_009961.2 CDS BPH1 NC_001135.4 179516 186019 D PProtein homologous to human Chediak-Higashi syndrome and murine Beige proteins, which are implicated in disease syndromes due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting; Bph1p 179516..186019 Saccharomyces cerevisiae 850398 NP_009962.2 CDS SNT1 NC_001135.4 186485 190165 D Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; Snt1p 186485..190165 Saccharomyces cerevisiae 850399 NP_009963.1 CDS FEN1 NC_001135.4 190588 191631 D Fen1p; Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway 190588..191631 Saccharomyces cerevisiae 850400 NP_009964.2 CDS RRP43 NC_001135.4 191830 193014 R Rrp43p; Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA complement(191830..193014) Saccharomyces cerevisiae 850401 NP_009965.2 CDS RBK1 NC_001135.4 193293 194294 D Rbk1p; Putative ribokinase 193293..194294 Saccharomyces cerevisiae 850402 NP_009966.2 CDS PHO87 NC_001135.4 194410 197181 R Pho87p; Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments complement(194410..197181) Saccharomyces cerevisiae 850403 NP_009967.2 CDS BUD5 NC_001135.4 197617 199545 R GTP/GDP exchange factor for Rsr1p (Bud1p) required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; Bud5p complement(197617..199545) Saccharomyces cerevisiae 850405 NP_009868.3 CDS MATALPHA2 NC_001135.4 199542 200174 R Matalpha2p; Homeobox-domain protein that, with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette complement(199542..200174) Saccharomyces cerevisiae 850406 NP_009869.3 CDS MATALPHA1 NC_001135.4 200438 200965 D Transcriptional co-activator involved in regulation of mating-type-specific gene expression; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette; Matalpha1p 200438..200965 Saccharomyces cerevisiae 850407 NP_009971.1 CDS TAF2 NC_001135.4 201170 205393 R Taf2p; TFIID subunit (150 kDa), involved in RNA polymerase II transcription initiation complement(201170..205393) Saccharomyces cerevisiae 850409 NP_009972.1 CDS YCR043C NC_001135.4 206257 206640 R Ycr043cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene complement(206257..206640) Saccharomyces cerevisiae 850410 NP_009973.1 CDS PER1 NC_001135.4 206873 207946 R Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1; Per1p complement(206873..207946) Saccharomyces cerevisiae 850411 NP_009974.1 CDS YCR045C NC_001135.4 208131 209606 R Ycr045cp; Putative protein of unknown function complement(208131..209606) Saccharomyces cerevisiae 850412 NP_009975.1 CDS IMG1 NC_001135.4 209910 210419 R Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome; Img1p complement(209910..210419) Saccharomyces cerevisiae 850413 NP_009976.1 CDS BUD23 NC_001135.4 210714 211541 R Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud23p complement(210714..211541) Saccharomyces cerevisiae 850414 NP_009978.1 CDS ARE1 NC_001135.4 211925 213757 D Are1p; Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen 211925..213757 Saccharomyces cerevisiae 850415 NP_009979.1 CDS YCR050C NC_001135.4 213460 213768 R Ycr050cp; Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein complement(213460..213768) Saccharomyces cerevisiae 850417 NP_009980.1 CDS YCR051W NC_001135.4 214067 214735 D Ycr051wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene 214067..214735 Saccharomyces cerevisiae 850418 NP_009981.1 CDS RSC6 NC_001135.4 214990 216441 D Rsc6p; Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p 214990..216441 Saccharomyces cerevisiae 850419 NP_009982.1 CDS THR4 NC_001135.4 216693 218237 D Threonine synthase, conserved protein that catalyzes formation of threonine from 0-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway; Thr4p 216693..218237 Saccharomyces cerevisiae 850420 NP_009983.1 CDS CTR86 NC_001135.4 218372 220063 R Essential protein of unknown function; upstream region contains a Gcn4p responsive site suggesting a possible role in amino acid biosynthesis; Ctr86p complement(218372..220063) Saccharomyces cerevisiae 850421 NP_009984.1 CDS PWP2 NC_001135.4 220453 223224 R Pwp2p; Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis complement(220453..223224) Saccharomyces cerevisiae 850422 NP_009985.1 CDS YIH1 NC_001135.4 223450 224226 R Yih1p; Protein that inhibits activation of Gcn2p, an eIF2 alpha subunit protein kinase, by competing for Gcn1p binding, thus impacting gene expression in response to starvation; has sequence and functional similarity to the mouse IMPACT gene complement(223450..224226) Saccharomyces cerevisiae 850423 NP_009986.1 CDS TAH1 NC_001135.4 224395 224730 D Tah1p; HSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2, contains a single TPR domain with at least two TPR motifs 224395..224730 Saccharomyces cerevisiae 850424 NP_009987.2 CDS YCR061W NC_001135.4 225559 227454 D Ycr061wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 225559..227454 Saccharomyces cerevisiae 850425 NP_009990.1 CDS BUD31 NC_001135.4 228314 228787 D Protein involved in bud-site selection; analysis of integrated high-throughput datasets predicts an involvement in RNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud31p 228314..228787 Saccharomyces cerevisiae 850427 NP_009991.2 CDS HCM1 NC_001135.4 229306 231000 D Hcm1p; Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role 229306..231000 Saccharomyces cerevisiae 850429 NP_009992.1 CDS RAD18 NC_001135.4 231496 232959 D Rad18p; Protein involved in postreplication repair; binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; forms heterodimer with Rad6p; contains RING-finger motif 231496..232959 Saccharomyces cerevisiae 850430 NP_009993.1 CDS SED4 NC_001135.4 233121 236318 R Sed4p; Integral endoplasmic reticulum membrane protein, functions as a positive regulator of Sar1p probably through inhibition of GTPase activation by Sec23p; binds Sec16p, participates in vesicle formation, similar to Sec12p complement(233121..236318) Saccharomyces cerevisiae 850431 NP_009994.2 CDS ATG15 NC_001135.4 237210 238772 D Lipase, required for intravacuolar lysis of autophagic bodies; located in the endoplasmic reticulum membrane and targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway; Atg15p 237210..238772 Saccharomyces cerevisiae 850432 NP_009995.1 CDS CPR4 NC_001135.4 239051 240007 D Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; Cpr4p 239051..240007 Saccharomyces cerevisiae 850433 NP_009996.2 CDS IMG2 NC_001135.4 240099 240539 R Mitochondrial ribosomal protein of the small subunit; Img2p complement(240099..240539) Saccharomyces cerevisiae 850434 NP_009997.2 CDS RSA4 NC_001135.4 240801 242348 R WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus; Rsa4p complement(240801..242348) Saccharomyces cerevisiae 850435 NP_009998.2 CDS SSK22 NC_001135.4 242585 246580 R Ssk22p; MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p complement(242585..246580) Saccharomyces cerevisiae 850436 NP_009999.2 CDS SOL2 NC_001135.4 246960 247907 D Sol2p; Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p 246960..247907 Saccharomyces cerevisiae 850437 NP_010000.1 CDS ERS1 NC_001135.4 248030 248812 R Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains; Ers1p complement(248030..248812) Saccharomyces cerevisiae 850438 NP_878058.1 CDS YCR075W-A NC_001135.4 248972 249199 D Ycr075w-ap; Putative protein of unknown function; identified by homology to Ashbya gossypii 248972..249199 Saccharomyces cerevisiae 1466399 NP_010001.1 CDS YCR076C NC_001135.4 249290 250042 R Ycr076cp; Putative protein of unknown function; YCR076C is not an essential gene complement(249290..250042) Saccharomyces cerevisiae 850439 NP_010002.2 CDS PAT1 NC_001135.4 250235 252625 R Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p; Pat1p complement(250235..252625) Saccharomyces cerevisiae 850440 NP_010003.2 CDS PTC6 NC_001135.4 252842 254170 D Ptc6p; Phosphoprotein phosphatase type 2C similar to mammalian PP1Ks; involved in mitophagy; localized to mitochondrial inner membrane space; null mutant is sensitive to rapamycin 252842..254170 Saccharomyces cerevisiae 850441 NP_010004.1 CDS SRB8 NC_001135.4 254368 258651 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression; Srb8p 254368..258651 Saccharomyces cerevisiae 850442 NP_010005.1 CDS AHC2 NC_001135.4 258880 259266 D Protein of unknown function, putative transcriptional regulator; proposed to be a Ada Histone acetyltransferase complex component; GFP tagged protein is localized to the cytoplasm and nucleus; Ahc2p 258880..259266 Saccharomyces cerevisiae 850443 NP_010006.1 CDS TRX3 NC_001135.4 259575 259958 D Trx3p; Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p 259575..259958 Saccharomyces cerevisiae 850444 NP_010007.1 CDS TUP1 NC_001135.4 260308 262449 R Tup1p; General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes complement(260308..262449) Saccharomyces cerevisiae 850445 NP_010009.1 CDS CSM1 NC_001135.4 263389 263961 D Nucleolar protein that forms a complex with Lrs4p which binds Mam1p at kinetochores during meiosis I to mediate accurate chromosome segregation, may be involved in premeiotic DNA replication; possible role in telomere maintenance; Csm1p 263389..263961 Saccharomyces cerevisiae 850447 NP_010011.1 CDS LUG1 NC_001135.4 264003 264464 R Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene; Lug1p complement(264003..264464) Saccharomyces cerevisiae 850449 NP_010012.1 CDS ABP1 NC_001135.4 265065 266843 D Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; Abp1p 265065..266843 Saccharomyces cerevisiae 850450 NP_010013.2 CDS FIG2 NC_001135.4 267431 272260 D Fig2p; Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating 267431..272260 Saccharomyces cerevisiae 850451 NP_010014.1 CDS YCR090C NC_001135.4 272312 272860 R Ycr090cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene complement(272312..272860) Saccharomyces cerevisiae 850452 NP_010015.2 CDS KIN82 NC_001135.4 274401 276563 D Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily; Kin82p 274401..276563 Saccharomyces cerevisiae 850453 NP_010016.2 CDS MSH3 NC_001135.4 276761 279817 R Msh3p; Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability complement(276761..279817) Saccharomyces cerevisiae 850454 NP_010017.2 CDS CDC39 NC_001135.4 280114 286440 D Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor; Cdc39p 280114..286440 Saccharomyces cerevisiae 850455 NP_010018.1 CDS CDC50 NC_001135.4 286759 287934 D Endosomal protein that regulates cell polarity, controls polarized growth; similar to Ynr048wp and Lem3p; Cdc50p 286759..287934 Saccharomyces cerevisiae 850456 NP_010019.1 CDS OCA4 NC_001135.4 288167 289255 R Oca4p; Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts complement(288167..289255) Saccharomyces cerevisiae 850457 NP_001018032.1 CDS YCR095W-A NC_001135.4 289636 289794 D Ycr095w-ap; Putative protein of unknown function 289636..289794 Saccharomyces cerevisiae 3077354 NP_010020.1 CDS HMRA2 NC_001135.4 293176 293535 R Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system; Hmra2p complement(293176..293535) Saccharomyces cerevisiae 850458 NP_010021.1 CDS HMRA1 NC_001135.4 293832 294318 D Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells; Hmra1p join(293832..293936,293991..294236,294289..294318) Saccharomyces cerevisiae 850459 NP_010022.1 CDS GIT1 NC_001135.4 297046 298602 R Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability; Git1p complement(297046..298602) Saccharomyces cerevisiae 850462 NP_010023.1 CDS YCR099C NC_001135.4 300829 301296 R Ycr099cp; Putative protein of unknown function complement(300829..301296) Saccharomyces cerevisiae 850463 NP_010024.1 CDS YCR100C NC_001135.4 301268 302218 R Ycr100cp; Putative protein of unknown function complement(301268..302218) Saccharomyces cerevisiae 850464 NP_010025.1 CDS YCR101C NC_001135.4 302479 303027 R Ycr101cp; Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene complement(302479..303027) Saccharomyces cerevisiae 850465 NP_010026.1 CDS YCR102C NC_001135.4 304358 305464 R Ycr102cp; Putative protein of unknown function; involved in copper metabolism; similar to C.carbonum toxD gene; YCR102C is not an essential gene complement(304358..305464) Saccharomyces cerevisiae 850466 NP_010029.1 CDS PAU3 NC_001135.4 307798 308172 D Pau3p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme 307798..308172 Saccharomyces cerevisiae 850468 NP_010030.1 CDS ADH7 NC_001135.4 309067 310152 D NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; Adh7p 309067..310152 Saccharomyces cerevisiae 850469 NP_010031.1 CDS RDS1 NC_001135.4 310955 313453 D Zinc cluster transcription factor involved in conferring resistance to cycloheximide; Rds1p 310955..313453 Saccharomyces cerevisiae 850470 NP_010032.1 CDS AAD3 NC_001135.4 313887 314978 D Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; Aad3p 313887..314978 Saccharomyces cerevisiae 850471 NP_878059.1 CDS YCR108C NC_001135.4 315994 316185 R Ycr108cp; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(315994..316185) Saccharomyces cerevisiae 1466400 NP_010033.1 CDS COS7 NC_001136.8 1802 2953 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Cos7p 1802..2953 Saccharomyces cerevisiae 851348 NP_010034.1 CDS MPH2 NC_001136.8 5985 7814 D Mph2p; Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication 5985..7814 Saccharomyces cerevisiae 851350 NP_010035.1 CDS SOR2 NC_001136.8 8683 9756 R Sor2p; Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in fructose or mannose metabolism complement(8683..9756) Saccharomyces cerevisiae 851351 NP_010036.1 CDS HXT15 NC_001136.8 11657 13360 R Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose; Hxt15p complement(11657..13360) Saccharomyces cerevisiae 851352 NP_010037.1 CDS THI13 NC_001136.8 16204 17226 D Thi13p; Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 16204..17226 Saccharomyces cerevisiae 851353 NP_010038.1 CDS AAD4 NC_001136.8 17577 18566 R Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase, involved in the oxidative stress response; Aad4p complement(17577..18566) Saccharomyces cerevisiae 851354 NP_010040.1 CDS YDL241W NC_001136.8 20635 21006 D Ydl241wp; Putative protein of unknown function; YDL241W is not an essential gene 20635..21006 Saccharomyces cerevisiae 851356 NP_010041.1 CDS LRG1 NC_001136.8 22823 25876 D Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis; Lrg1p 22823..25876 Saccharomyces cerevisiae 851358 NP_010042.1 CDS ADY3 NC_001136.8 26403 28775 R Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; Ady3p complement(26403..28775) Saccharomyces cerevisiae 851359 NP_010043.1 CDS GUD1 NC_001136.8 28985 30454 R Gud1p; Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures complement(28985..30454) Saccharomyces cerevisiae 851360 NP_010044.1 CDS LRC1 NC_001136.8 30657 31829 D Putative protein of unknown function; YDL237W is not an essential gene; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Lrc1p 30657..31829 Saccharomyces cerevisiae 851361 NP_010045.1 CDS PHO13 NC_001136.8 32296 33234 D Pho13p; Alkaline phosphatase specific for p-nitrophenyl phosphate, involved in dephosphorylation of histone II-A and casein 32296..33234 Saccharomyces cerevisiae 851362 NP_010046.1 CDS YPD1 NC_001136.8 33415 33918 R Ypd1p; Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus complement(33415..33918) Saccharomyces cerevisiae 851363 NP_010047.1 CDS GYP7 NC_001136.8 34238 36478 R Gyp7p; GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking complement(34238..36478) Saccharomyces cerevisiae 851364 NP_010048.1 CDS YDL233W NC_001136.8 36798 38174 D Ydl233wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL233W is not an essential gene 36798..38174 Saccharomyces cerevisiae 851365 NP_010049.1 CDS OST4 NC_001136.8 38488 38598 D Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex; Ost4p 38488..38598 Saccharomyces cerevisiae 851366 NP_010050.1 CDS BRE4 NC_001136.8 38868 42245 R Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport; Bre4p complement(38868..42245) Saccharomyces cerevisiae 851367 NP_010051.1 CDS PTP1 NC_001136.8 42701 43708 D Ptp1p; Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria 42701..43708 Saccharomyces cerevisiae 851368 NP_010052.1 CDS SSB1 NC_001136.8 44066 45907 D Ssb1p; Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p 44066..45907 Saccharomyces cerevisiae 851369 NP_010054.1 CDS HO NC_001136.8 46272 48032 R Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p; Hop complement(46272..48032) Saccharomyces cerevisiae 851371 NP_010055.1 CDS GCS1 NC_001136.8 51116 52174 R Gcs1p; ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p complement(51116..52174) Saccharomyces cerevisiae 851372 NP_010056.1 CDS SHS1 NC_001136.8 52446 54101 D Shs1p; One of five related septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p, Shs1p) that form a cortical filamentous collar at the mother-bud neck which is necessary for normal morphogenesis and cytokinesis 52446..54101 Saccharomyces cerevisiae 851373 NP_010057.1 CDS WHI4 NC_001136.8 54398 56347 R Whi4p; Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division complement(54398..56347) Saccharomyces cerevisiae 851302 NP_010058.1 CDS HBT1 NC_001136.8 57266 60406 R Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; Hbt1p complement(57266..60406) Saccharomyces cerevisiae 851303 NP_010059.1 CDS FMP45 NC_001136.8 60873 61802 R Integral membrane protein localized to mitochondria (untagged protein) and eisosomes, immobile patches at the cortex associated with endocytosis; sporulation and sphingolipid content are altered in mutants; has homologs SUR7 and YNL194C; Fmp45p complement(60873..61802) Saccharomyces cerevisiae 851304 NP_010061.1 CDS CDC13 NC_001136.8 62245 65019 R Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; Cdc13p complement(62245..65019) Saccharomyces cerevisiae 851306 NP_010062.1 CDS DTD1 NC_001136.8 65243 65766 D Dtd1p; D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes join(65243..65307,65379..65766) Saccharomyces cerevisiae 851307 NP_010063.1 CDS YDL218W NC_001136.8 66494 67447 D Ydl218wp; Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions 66494..67447 Saccharomyces cerevisiae 851308 NP_010064.1 CDS TIM22 NC_001136.8 67984 68607 R Tim22p; Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane complement(67984..68607) Saccharomyces cerevisiae 851309 NP_010065.2 CDS RRI1 NC_001136.8 68998 70320 R Rri1p; Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling complement(68998..70320) Saccharomyces cerevisiae 851310 NP_010066.1 CDS GDH2 NC_001136.8 70641 73919 R NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; Gdh2p complement(70641..73919) Saccharomyces cerevisiae 851311 NP_010067.1 CDS PRR2 NC_001136.8 74447 76546 R Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; Prr2p complement(74447..76546) Saccharomyces cerevisiae 851312 NP_010068.1 CDS NOP6 NC_001136.8 77290 77967 R Putative RNA-binding protein implicated in ribosome biogenesis; contains an RNA recognition motif (RRM) and has similarity to hydrophilins; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes; Nop6p complement(77290..77967) Saccharomyces cerevisiae 851313 NP_010069.1 CDS SHR3 NC_001136.8 78427 79059 D Shr3p; Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface 78427..79059 Saccharomyces cerevisiae 851314 NP_010070.1 CDS YDL211C NC_001136.8 79295 80413 R Ydl211cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole complement(79295..80413) Saccharomyces cerevisiae 851315 NP_010071.1 CDS UGA4 NC_001136.8 84271 85986 D Uga4p; Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane 84271..85986 Saccharomyces cerevisiae 851317 NP_010072.1 CDS CWC2 NC_001136.8 86208 87227 R Protein involved in pre-mRNA splicing, component of a complex containing Cef1p; interacts with Prp19p; contains an RNA recognition motif; has similarity to S. pombe Cwf2p; Cwc2p complement(86208..87227) Saccharomyces cerevisiae 851318 NP_010073.2 CDS NHP2 NC_001136.8 87513 87983 D Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing; Nhp2p 87513..87983 Saccharomyces cerevisiae 851319 NP_010074.1 CDS GLE1 NC_001136.8 88249 89865 D Gle1p; Cytoplasmic nucleoporin required for polyadenylated RNA export but not for protein import; component of Nup82p nuclear pore subcomplex; contains a nuclear export signal 88249..89865 Saccharomyces cerevisiae 851320 NP_010075.1 CDS YDL206W NC_001136.8 90177 92465 D Ydl206wp; Putative protein of unknown function; YDL206W is not an essential protein 90177..92465 Saccharomyces cerevisiae 851321 NP_010076.1 CDS HEM3 NC_001136.8 92763 93746 R Phorphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p; Hem3p complement(92763..93746) Saccharomyces cerevisiae 851322 NP_010077.1 CDS RTN2 NC_001136.8 94606 95787 D Rtn2p; Protein of unknown function; has similarity to mammalian reticulon proteins; member of the RTNLA (reticulon-like A) subfamily 94606..95787 Saccharomyces cerevisiae 851323 NP_010078.1 CDS ACK1 NC_001136.8 96083 97954 R Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria; Ack1p complement(96083..97954) Saccharomyces cerevisiae 851324 NP_010079.1 CDS MRPL11 NC_001136.8 98476 99225 D Mrpl11p; Mitochondrial ribosomal protein of the large subunit 98476..99225 Saccharomyces cerevisiae 851325 NP_010080.1 CDS TRM8 NC_001136.8 99562 100422 D Trm8p; Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA 99562..100422 Saccharomyces cerevisiae 851326 NP_010081.2 CDS MGT1 NC_001136.8 100502 101068 R Mgt1p; DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage complement(100502..101068) Saccharomyces cerevisiae 851327 NP_010082.1 CDS YDL199C NC_001136.8 101291 103354 R Ydl199cp; Putative transporter, member of the sugar porter family complement(101291..103354) Saccharomyces cerevisiae 851328 NP_010083.1 CDS GGC1 NC_001136.8 103650 104552 R Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family; Ggc1p complement(103650..104552) Saccharomyces cerevisiae 851329 NP_010084.1 CDS ASF2 NC_001136.8 104918 106495 R Asf2p; Anti-silencing protein that causes derepression of silent loci when overexpressed complement(104918..106495) Saccharomyces cerevisiae 851330 NP_010086.1 CDS SEC31 NC_001136.8 107209 111030 D Sec31p; Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation 107209..111030 Saccharomyces cerevisiae 851332 NP_010087.1 CDS SNF3 NC_001136.8 111581 114235 D Snf3p; Plasma membrane glucose sensor that regulates glucose transport; has 12 predicted transmembrane segments; long cytoplasmic C-terminal tail is required for low glucose induction of hexose transporter genes HXT2 and HXT4 111581..114235 Saccharomyces cerevisiae 851333 NP_010088.1 CDS NUS1 NC_001136.8 114673 115800 D Prenyltransferase, required for cell viability; involved in protein trafficking; Nus1p 114673..115800 Saccharomyces cerevisiae 851334 NP_010089.1 CDS ARF1 NC_001136.8 116322 116867 D Arf1p; ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p 116322..116867 Saccharomyces cerevisiae 851335 NP_010090.1 CDS RPL35A NC_001136.8 117665 118518 D Rpl35ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl35Bp and has similarity to rat L35 ribosomal protein join(117665..117667,118159..118518) Saccharomyces cerevisiae 851336 NP_010091.1 CDS UFD2 NC_001136.8 118708 121593 R Ufd2p; Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 complement(118708..121593) Saccharomyces cerevisiae 851337 NP_010092.2 CDS RBS1 NC_001136.8 122217 123590 D Rbs1p; Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain 122217..123590 Saccharomyces cerevisiae 851338 NP_010093.1 CDS PPH22 NC_001136.8 123866 124999 R Pph22p; Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis complement(123866..124999) Saccharomyces cerevisiae 851339 NP_010095.1 CDS YDL186W NC_001136.8 125617 126450 D Ydl186wp; Putative protein of unknown function; YDL186W is not an essential gene 125617..126450 Saccharomyces cerevisiae 851340 NP_010096.1 CDS TFP1 NC_001136.8 126788 130003 D Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; Tfp1p 126788..130003 Saccharomyces cerevisiae 851342 NP_010097.1 CDS RPL41A NC_001136.8 130408 130485 R Rpl41ap; Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Bp and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable complement(130408..130485) Saccharomyces cerevisiae 851344 NP_010098.1 CDS YDL183C NC_001136.8 130872 131834 R Ydl183cp; Putative protein of unknown function; YDL183C is not an essential gene complement(130872..131834) Saccharomyces cerevisiae 851345 NP_010099.1 CDS LYS20 NC_001136.8 133438 134724 D Lys20p; Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p 133438..134724 Saccharomyces cerevisiae 851346 NP_010100.1 CDS INH1 NC_001136.8 135180 135437 D Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p and both Inh1p and Stf1p exhibit the potential to form coiled-coil structures; Inh1p 135180..135437 Saccharomyces cerevisiae 851347 NP_010101.1 CDS YDL180W NC_001136.8 135897 137540 D Ydl180wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 135897..137540 Saccharomyces cerevisiae 851374 NP_010102.1 CDS PCL9 NC_001136.8 138292 139206 D Pcl9p; Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p 138292..139206 Saccharomyces cerevisiae 851375 NP_010103.1 CDS DLD2 NC_001136.8 139523 141115 D Dld2p; D-lactate dehydrogenase, located in the mitochondrial matrix 139523..141115 Saccharomyces cerevisiae 851376 NP_010104.1 CDS YDL177C NC_001136.8 141210 141722 R Ydl177cp; Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene complement(141210..141722) Saccharomyces cerevisiae 851377 NP_010105.1 CDS YDL176W NC_001136.8 142098 144224 D Ydl176wp; Putative protein of unknown function; YDL176W is not an essential gene 142098..144224 Saccharomyces cerevisiae 851378 NP_010106.1 CDS AIR2 NC_001136.8 144485 145519 R RING finger protein that interacts with the arginine methyltransferase Hmt1p; may regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air1p; Air2p complement(144485..145519) Saccharomyces cerevisiae 851379 NP_010107.1 CDS DLD1 NC_001136.8 145827 147590 R Dld1p; D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane complement(145827..147590) Saccharomyces cerevisiae 851380 NP_010108.1 CDS YDL173W NC_001136.8 148192 149079 D Ydl173wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDL173W is not an essential gene 148192..149079 Saccharomyces cerevisiae 851381 NP_010110.1 CDS GLT1 NC_001136.8 149204 155641 R NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; Glt1p complement(149204..155641) Saccharomyces cerevisiae 851383 NP_010111.1 CDS UGA3 NC_001136.8 156319 157905 D Uga3p; Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus 156319..157905 Saccharomyces cerevisiae 851384 NP_010112.1 CDS UGX2 NC_001136.8 158065 158736 R Ugx2p; Protein of unknown function, transcript accumulates in response to any combination of stress conditions complement(158065..158736) Saccharomyces cerevisiae 851385 NP_010113.1 CDS SFA1 NC_001136.8 159605 160765 D Sfa1p; Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p 159605..160765 Saccharomyces cerevisiae 851386 NP_010114.1 CDS NRP1 NC_001136.8 160996 163155 R Protein of unknown function, rich in asparagine residues; Nrp1p complement(160996..163155) Saccharomyces cerevisiae 851387 NP_010115.1 CDS FAP7 NC_001136.8 163450 164043 R Fap7p; Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D complement(163450..164043) Saccharomyces cerevisiae 851388 NP_010116.1 CDS CDC36 NC_001136.8 164291 164866 D Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor; Cdc36p 164291..164866 Saccharomyces cerevisiae 851389 NP_010117.1 CDS CDC9 NC_001136.8 164988 167255 R DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination; Cdc9p complement(164988..167255) Saccharomyces cerevisiae 851391 NP_010120.1 CDS ENT1 NC_001136.8 167715 169079 D Epsin-like protein involved in endocytosis and actin patch assembly and functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; Ent1p 167715..169079 Saccharomyces cerevisiae 851392 NP_878060.1 CDS YDL160C-A NC_001136.8 169367 169609 R Ydl160c-ap; Putative protein of unknown function complement(169367..169609) Saccharomyces cerevisiae 1466431 NP_010121.1 CDS DHH1 NC_001136.8 170411 171931 R Dhh1p; Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation complement(170411..171931) Saccharomyces cerevisiae 851394 NP_076884.1 CDS YDL159W-A NC_001136.8 172183 172314 D Ydl159w-ap; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species 172183..172314 Saccharomyces cerevisiae 851395 NP_010122.1 CDS STE7 NC_001136.8 172482 174029 D Ste7p; Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway 172482..174029 Saccharomyces cerevisiae 851396 NP_010124.1 CDS YDL157C NC_001136.8 174233 174589 R Ydl157cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(174233..174589) Saccharomyces cerevisiae 851398 NP_010125.1 CDS YDL156W NC_001136.8 174919 176487 D Ydl156wp; Putative protein of unknown function; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 174919..176487 Saccharomyces cerevisiae 851399 NP_010126.1 CDS CLB3 NC_001136.8 176774 178057 D Clb3p; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation 176774..178057 Saccharomyces cerevisiae 851400 NP_010127.1 CDS MSH5 NC_001136.8 178335 181040 D Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans; Msh5p 178335..181040 Saccharomyces cerevisiae 851401 NP_010128.1 CDS SAS10 NC_001136.8 181187 183019 R Sas10p; Component of the small (ribosomal) subunit (SSU) processosome required for pre-18S rRNa processing; essential nucleolar protein that, when overproduced, disrupts silencing complement(181187..183019) Saccharomyces cerevisiae 851403 NP_010131.1 CDS RPC53 NC_001136.8 183344 184612 D Rpc53p; RNA polymerase III subunit C53 183344..184612 Saccharomyces cerevisiae 851404 NP_010132.1 CDS ATG9 NC_001136.8 184926 187919 D Transmembrane protein involved in formation of Cvt and autophagic vesicles; cycles between the pre-autophagosomal structure and other cytosolic punctate structures, not found in autophagosomes; Atg9p 184926..187919 Saccharomyces cerevisiae 851406 NP_010133.1 CDS NOP14 NC_001136.8 188155 190587 R Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA; Nop14p complement(188155..190587) Saccharomyces cerevisiae 851407 NP_010134.1 CDS RPN5 NC_001136.8 190925 192262 D Rpn5p; Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p 190925..192262 Saccharomyces cerevisiae 851408 NP_010135.1 CDS LDB17 NC_001136.8 192751 194226 D Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface; Ldb17p 192751..194226 Saccharomyces cerevisiae 851409 NP_010136.1 CDS COP1 NC_001136.8 194572 198177 R Cop1p; Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway complement(194572..198177) Saccharomyces cerevisiae 851410 NP_010137.2 CDS YDL144C NC_001136.8 198663 199733 R Ydl144cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. complement(198663..199733) Saccharomyces cerevisiae 851411 NP_010138.1 CDS CCT4 NC_001136.8 199997 201583 D Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct4p 199997..201583 Saccharomyces cerevisiae 851412 NP_010139.1 CDS CRD1 NC_001136.8 201720 202571 R Crd1p; Cardiolipin synthase; produces cardiolipin, which is an important constituent of mitochondrial membranes; required for normal mitochondrial membrane potential and function complement(201720..202571) Saccharomyces cerevisiae 851413 NP_010140.1 CDS BPL1 NC_001136.8 203040 205112 D Bpl1p; Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation 203040..205112 Saccharomyces cerevisiae 851414 NP_010141.1 CDS RPO21 NC_001136.8 205361 210562 R Rpo21p; RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime complement(205361..210562) Saccharomyces cerevisiae 851415 NP_010142.2 CDS SCM3 NC_001136.8 211376 212047 R Scm3p; Nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; contains nuclear export signal (NES); required for G2/M progression and localization of Cse4p complement(211376..212047) Saccharomyces cerevisiae 851416 NP_010143.1 CDS RGT2 NC_001136.8 213352 215643 D Rgt2p; Plasma membrane glucose receptor, highly similar to Snf3p; both Rgt2p and Snf3p serve as transmembrane glucose sensors generating an intracellular signal that induces expression of glucose transporter (HXT) genes 213352..215643 Saccharomyces cerevisiae 851417 NP_010144.1 CDS ARF2 NC_001136.8 216529 217074 D Arf2p; ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p 216529..217074 Saccharomyces cerevisiae 851418 NP_010145.1 CDS RPL35B NC_001136.8 217600 218367 D Rpl35bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl35Ap and has similarity to rat L35 ribosomal protein join(217600..217602,218008..218367) Saccharomyces cerevisiae 851419 NP_010146.1 CDS RDI1 NC_001136.8 218680 219288 R Rho GDP dissociation inhibitor involved in the localization and regulation of Cdc42p; Rdi1p complement(218680..219288) Saccharomyces cerevisiae 851420 NP_010147.1 CDS PPH21 NC_001136.8 219662 220771 R Pph21p; Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis complement(219662..220771) Saccharomyces cerevisiae 851421 NP_010148.1 CDS RPL41B NC_001136.8 221724 221801 R Rpl41bp; Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable complement(221724..221801) Saccharomyces cerevisiae 851422 NP_010149.1 CDS YDL133W NC_001136.8 222427 223740 D Ydl133wp; Putative protein of unknown function 222427..223740 Saccharomyces cerevisiae 851423 NP_010150.1 CDS CDC53 NC_001136.8 224304 226751 D Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; Cdc53p 224304..226751 Saccharomyces cerevisiae 851424 NP_010151.1 CDS LYS21 NC_001136.8 227393 228715 D Lys21p; Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p 227393..228715 Saccharomyces cerevisiae 851425 NP_010152.1 CDS STF1 NC_001136.8 229171 229431 D Stf1p; Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein 229171..229431 Saccharomyces cerevisiae 851426 NP_010153.1 CDS RPP1B NC_001136.8 229906 230527 D Rpp1bp; Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component join(229906..230019,230321..230527) Saccharomyces cerevisiae 851427 NP_010154.1 CDS YDL129W NC_001136.8 231024 231899 D Ydl129wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene 231024..231899 Saccharomyces cerevisiae 851428 NP_010155.1 CDS VCX1 NC_001136.8 232652 233887 D Vcx1p; Vacuolar H+/Ca2+ exchanger involved in control of cytosolic Ca2+ concentration; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter 232652..233887 Saccharomyces cerevisiae 851429 NP_010156.2 CDS PCL2 NC_001136.8 234927 235853 D Pcl2p; G1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk) to contribute to entry into the mitotic cell cycle, essential for cell morphogenesis; localizes to sites of polarized cell growth 234927..235853 Saccharomyces cerevisiae 851430 NP_010157.1 CDS CDC48 NC_001136.8 236157 238664 R Cdc48p; ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome complement(236157..238664) Saccharomyces cerevisiae 851431 NP_010158.1 CDS HNT1 NC_001136.8 239019 239606 R Hnt1p; Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint complement(join(239019..239398,239510..239606)) Saccharomyces cerevisiae 851432 NP_010159.1 CDS YDL124W NC_001136.8 240259 241197 D Ydl124wp; NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family 240259..241197 Saccharomyces cerevisiae 851433 NP_010160.1 CDS SNA4 NC_001136.8 241418 241840 D Sna4p; Protein of unknown function, localized to the vacuolar outer membrane 241418..241840 Saccharomyces cerevisiae 851434 NP_010161.1 CDS UBP1 NC_001136.8 242552 244981 D Ubp1p; Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains 242552..244981 Saccharomyces cerevisiae 851435 NP_010162.1 CDS YDL121C NC_001136.8 245133 245582 R Ydl121cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic retiuculum; YDL121C is not an essential protein complement(245133..245582) Saccharomyces cerevisiae 851436 NP_010163.1 CDS YFH1 NC_001136.8 245923 246447 D Yfh1p; Frataxin, regulates mitochondrial iron accumulation; interacts with Isu1p which promotes Fe-S cluster assembly; interacts with electron transport chain components and may influence respiration; human homolog involved in Friedrich's ataxia 245923..246447 Saccharomyces cerevisiae 851437 NP_010164.1 CDS YDL119C NC_001136.8 246689 247612 R Ydl119cp; Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria complement(246689..247612) Saccharomyces cerevisiae 851439 NP_010165.1 CDS YDL118W NC_001136.8 247302 247682 D Ydl118wp; Non-essential protein of unconfirmed function; mutants are defective in telomere maintenance, and are synthetically sick or lethal with alpha-synuclein 247302..247682 Saccharomyces cerevisiae 851438 NP_010166.1 CDS CYK3 NC_001136.8 248581 251238 D SH3-domain protein located in the mother-bud neck and the cytokinetic actin ring; mutant phenotype and genetic interactions suggest a role in cytokinesis; Cyk3p 248581..251238 Saccharomyces cerevisiae 851440 NP_010167.1 CDS NUP84 NC_001136.8 251566 253746 D Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis; Nup84p 251566..253746 Saccharomyces cerevisiae 851441 NP_010168.4 CDS IWR1 NC_001136.8 253995 255126 R Protein of unknown function, deletion causes hypersensitivity to the K1 killer toxin; Iwr1p complement(join(253995..254974,255045..255126)) Saccharomyces cerevisiae 851442 NP_010169.1 CDS YDL114W NC_001136.8 255604 256530 D Ydl114wp; Putative protein of unknown function with similarity to acyl-carrier-protein reductases; YDL114W is not an essential gene 255604..256530 Saccharomyces cerevisiae 851444 NP_010170.1 CDS ATG20 NC_001136.8 256633 258555 R Protein required for transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway; binds phosphatidylinositol-3-phosphate, involved in localization of membranes to the preautophagosome, potential Cdc28p substrate; Atg20p complement(256633..258555) Saccharomyces cerevisiae 851445 NP_010171.1 CDS TRM3 NC_001136.8 258915 263225 D Trm3p; 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs 258915..263225 Saccharomyces cerevisiae 851446 NP_010172.1 CDS RRP42 NC_001136.8 263313 264110 R Rrp42p; Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp4p, Rrp41p, Rrp43p and Dis3p complement(263313..264110) Saccharomyces cerevisiae 851447 NP_010173.1 CDS TMA17 NC_001136.8 264512 264964 R Tma17p; Protein of unknown function that associates with ribosomes complement(264512..264964) Saccharomyces cerevisiae 851448 NP_010174.1 CDS YDL109C NC_001136.8 265258 267201 R Ydl109cp; Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene complement(265258..267201) Saccharomyces cerevisiae 851449 NP_010175.1 CDS KIN28 NC_001136.8 267698 268699 D Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; Kin28p join(267698..267725,267807..268699) Saccharomyces cerevisiae 851450 NP_010176.1 CDS MSS2 NC_001136.8 268921 269976 D Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; Mss2p 268921..269976 Saccharomyces cerevisiae 851451 NP_010177.1 CDS PHO2 NC_001136.8 270222 271901 R Pho2p; Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p complement(270222..271901) Saccharomyces cerevisiae 851452 NP_010178.1 CDS NSE4 NC_001136.8 272389 273597 D Nuclear protein that plays a role in the function of the Smc5p-Rhc18p complex; Nse4p 272389..273597 Saccharomyces cerevisiae 851453 NP_010179.1 CDS QRI7 NC_001136.8 273653 274876 R Qri7p; Putative metalloprotease, similar to O-sialoglycoprotein metallopeptidase from P. haemolytica; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(273653..274876) Saccharomyces cerevisiae 851454 NP_010180.1 CDS QRI1 NC_001136.8 275148 276581 R UDP-N-acetylglucosamine pyrophosphorylase, catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; Qri1p complement(275148..276581) Saccharomyces cerevisiae 851455 NP_010181.1 CDS POL3 NC_001136.8 276872 280165 D Pol3p; Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) 276872..280165 Saccharomyces cerevisiae 851456 NP_010182.1 CDS DUN1 NC_001136.8 280307 281848 R Dun1p; Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair complement(280307..281848) Saccharomyces cerevisiae 851457 NP_010183.1 CDS GET3 NC_001136.8 282112 283176 R Get3p; ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress complement(282112..283176) Saccharomyces cerevisiae 851458 NP_010184.1 CDS BUG1 NC_001136.8 283419 284444 D Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; Bug1p 283419..284444 Saccharomyces cerevisiae 851459 NP_010185.1 CDS SNU23 NC_001136.8 284581 285165 R Snu23p; Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome complement(284581..285165) Saccharomyces cerevisiae 851460 NP_010186.1 CDS RPN6 NC_001136.8 285391 286695 R Rpn6p; Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion complement(285391..286695) Saccharomyces cerevisiae 851461 NP_010188.1 CDS PMT1 NC_001136.8 287059 289512 D Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt2p, can instead interact with Pmt3p in some conditions; target for new antifungals; Pmt1p 287059..289512 Saccharomyces cerevisiae 851462 NP_010190.1 CDS PMT5 NC_001136.8 289909 292140 D Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals; Pmt5p 289909..292140 Saccharomyces cerevisiae 851464 NP_010191.1 CDS SRP14 NC_001136.8 292781 293221 D Srp14p; Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 292781..293221 Saccharomyces cerevisiae 851466 NP_010192.1 CDS UBX3 NC_001136.8 293392 294759 R Ubx3p; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(293392..294759) Saccharomyces cerevisiae 851467 NP_010193.1 CDS RAM1 NC_001136.8 295034 296329 R Ram1p; Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit complement(295034..296329) Saccharomyces cerevisiae 851468 NP_010194.1 CDS YDL089W NC_001136.8 296820 298274 D Ydl089wp; Protein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate 296820..298274 Saccharomyces cerevisiae 851469 NP_010195.1 CDS ASM4 NC_001136.8 298417 300003 R Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p; Asm4p complement(298417..300003) Saccharomyces cerevisiae 851470 NP_010196.1 CDS LUC7 NC_001136.8 300214 300999 R Luc7p; Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA complement(300214..300999) Saccharomyces cerevisiae 851471 NP_010197.1 CDS YDL086W NC_001136.8 301413 302234 D Ydl086wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene 301413..302234 Saccharomyces cerevisiae 851472 NP_075208.1 CDS YDL085C-A NC_001136.8 302464 302670 R Ydl085c-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(302464..302670) Saccharomyces cerevisiae 851473 NP_010198.1 CDS NDE2 NC_001136.8 303211 304848 D Nde2p; Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain 303211..304848 Saccharomyces cerevisiae 851474 NP_010199.1 CDS SUB2 NC_001136.8 305237 306577 D Sub2p; Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56 305237..306577 Saccharomyces cerevisiae 851475 NP_010200.1 CDS RPS16B NC_001136.8 306926 307789 R Rps16bp; Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins complement(join(306926..307333,307766..307789)) Saccharomyces cerevisiae 851476 NP_010201.1 CDS RPL13A NC_001136.8 308424 309388 D Rpl13ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential for viability; has similarity to rat L13 ribosomal protein join(308424..308427,308793..309388) Saccharomyces cerevisiae 851477 NP_010202.1 CDS RPP1A NC_001136.8 309802 310122 R Rpp1ap; Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component complement(309802..310122) Saccharomyces cerevisiae 851478 NP_010203.1 CDS THI3 NC_001136.8 310642 312471 R Thi3p; Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis complement(310642..312471) Saccharomyces cerevisiae 851479 NP_010204.1 CDS MRK1 NC_001136.8 312951 314748 R Mrk1p; Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation complement(join(312951..314044,314337..314748)) Saccharomyces cerevisiae 851480 NP_010205.1 CDS MDH3 NC_001136.8 315357 316388 R Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle; Mdh3p complement(315357..316388) Saccharomyces cerevisiae 851481 NP_010206.1 CDS VAM6 NC_001136.8 316971 320120 R Vam6p; Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p complement(316971..320120) Saccharomyces cerevisiae 851482 NP_010207.1 CDS RXT3 NC_001136.8 320668 321552 R Subunit of the RPD3L complex; involved in histone deacetylation; Rxt3p complement(320668..321552) Saccharomyces cerevisiae 851483 NP_010208.1 CDS RPL31A NC_001136.8 322226 322988 D Rpl31ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p join(322226..322282,322704..322988) Saccharomyces cerevisiae 851484 NP_010209.1 CDS BRE1 NC_001136.8 324047 326149 R E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain; Bre1p complement(324047..326149) Saccharomyces cerevisiae 851485 NP_010210.1 CDS YDL073W NC_001136.8 326613 329567 D Ydl073wp; Putative protein of unknown function; YDL073W is not an essential gene 326613..329567 Saccharomyces cerevisiae 851486 NP_010211.1 CDS YET3 NC_001136.8 329836 330447 R Yet3p; Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein complement(329836..330447) Saccharomyces cerevisiae 851487 NP_010213.1 CDS BDF2 NC_001136.8 331025 332941 D Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; Bdf2p 331025..332941 Saccharomyces cerevisiae 851488 NP_010214.2 CDS CBS1 NC_001136.8 333121 333810 R Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader; Cbs1p complement(333121..333810) Saccharomyces cerevisiae 851491 NP_010216.1 CDS COX9 NC_001136.8 334217 334396 R Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; Cox9p complement(334217..334396) Saccharomyces cerevisiae 851492 NP_010217.1 CDS IDP1 NC_001136.8 334835 336121 D Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes; Idp1p 334835..336121 Saccharomyces cerevisiae 851493 NP_010218.2 CDS PEX19 NC_001136.8 336249 337277 R Pex19p; Chaperone and import receptor for newly-synthesized class I peroxisomal membrane proteins (PMPs), binds PMPs in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane complement(336249..337277) Saccharomyces cerevisiae 851494 NP_010219.1 CDS UBC9 NC_001136.8 337487 338070 D Ubc9p; SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) join(337487..337524,337635..338070) Saccharomyces cerevisiae 851495 NP_010220.1 CDS YDL063C NC_001136.8 338272 340134 R Ydl063cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL063C is not an essential gene complement(338272..340134) Saccharomyces cerevisiae 851497 NP_010222.1 CDS RPS29B NC_001136.8 340628 340798 R Rps29bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat S29 and E. coli S14 ribosomal proteins complement(340628..340798) Saccharomyces cerevisiae 851498 NP_010223.1 CDS TSR1 NC_001136.8 341619 343985 D Tsr1p; Protein required for processing of 20S pre-rRNA in the cytoplasm, associates with pre-40S ribosomal particles 341619..343985 Saccharomyces cerevisiae 851499 NP_010224.1 CDS RAD59 NC_001136.8 344237 344953 R Rad59p; Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p complement(344237..344953) Saccharomyces cerevisiae 851500 NP_010225.1 CDS USO1 NC_001136.8 345665 351037 D Uso1p; Essential protein involved in intracellular protein transport, coiled-coil protein necessary for transport from ER to Golgi; required for assembly of the ER-to-Golgi SNARE complex 345665..351037 Saccharomyces cerevisiae 851501 NP_010226.1 CDS YDL057W NC_001136.8 351434 352420 D Ydl057wp; Putative protein of unknown function; YDL057W is not an essential gene 351434..352420 Saccharomyces cerevisiae 851502 NP_010227.1 CDS MBP1 NC_001136.8 352877 355378 D Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes; Mbp1p 352877..355378 Saccharomyces cerevisiae 851503 NP_010228.1 CDS PSA1 NC_001136.8 355674 356759 R Psa1p; GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure complement(355674..356759) Saccharomyces cerevisiae 851504 NP_010229.1 CDS MCH1 NC_001136.8 359825 361285 R Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport; Mch1p complement(359825..361285) Saccharomyces cerevisiae 851506 NP_010230.1 CDS PBP4 NC_001136.8 361699 362256 R Pbp4p; Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay complement(361699..362256) Saccharomyces cerevisiae 851507 NP_010231.1 CDS SLC1 NC_001136.8 362672 363583 R Slc1p; 1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; located in lipid particles and endoplasmic reticulum complement(362672..363583) Saccharomyces cerevisiae 851508 NP_010232.1 CDS LHP1 NC_001136.8 363952 364779 D RNA binding protein required for maturation of tRNA and snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen; Lhp1p 363952..364779 Saccharomyces cerevisiae 851509 NP_010234.1 CDS KNH1 NC_001136.8 365068 365874 R Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; Knh1p complement(365068..365874) Saccharomyces cerevisiae 851511 NP_010235.1 CDS STP4 NC_001136.8 366739 368211 R Stp4p; Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p complement(366739..368211) Saccharomyces cerevisiae 851512 NP_010236.1 CDS SIT4 NC_001136.8 369769 370704 D Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; Sit4p 369769..370704 Saccharomyces cerevisiae 851513 NP_010237.1 CDS NPC2 NC_001136.8 371237 371758 D Npc2p; Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes 371237..371758 Saccharomyces cerevisiae 851514 NP_010238.1 CDS MRP10 NC_001136.8 372245 372532 D Mrp10p; Mitochondrial ribosomal protein of the small subunit 372245..372532 Saccharomyces cerevisiae 851515 NP_010239.1 CDS FAD1 NC_001136.8 372685 373605 R Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin; Fad1p complement(372685..373605) Saccharomyces cerevisiae 851516 NP_010240.1 CDS MTF2 NC_001136.8 373964 375286 R Mtf2p; Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription complement(373964..375286) Saccharomyces cerevisiae 851517 NP_010241.1 CDS PRP11 NC_001136.8 375677 376477 R Subunit of the SF3a splicing factor complex, required for spliceosome assembly; Prp11p complement(375677..376477) Saccharomyces cerevisiae 851518 NP_010242.1 CDS SIR2 NC_001136.8 376754 378442 R Sir2p; Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication complement(376754..378442) Saccharomyces cerevisiae 851520 NP_010244.1 CDS NAT1 NC_001136.8 378871 381435 R Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing; Nat1p complement(378871..381435) Saccharomyces cerevisiae 851521 NP_010245.1 CDS PRM7 NC_001136.8 381983 382330 R Prm7p; Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements complement(381983..382330) Saccharomyces cerevisiae 851522 NP_010246.1 CDS YDL038C NC_001136.8 382327 384078 R Ydl038cp; Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) complement(382327..384078) Saccharomyces cerevisiae 851523 NP_010247.1 CDS BSC1 NC_001136.8 384598 385584 R Protein of unconfirmed function, similar to cell surface flocculin Muc1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; Bsc1p complement(384598..385584) Saccharomyces cerevisiae 851524 NP_010248.1 CDS PUS9 NC_001136.8 387510 388898 R Pus9p; Mitochondrial tRNA pseudouridine synthase involved in pseudouridylation of mitochondrial tRNAs at position 32 complement(387510..388898) Saccharomyces cerevisiae 851525 NP_010249.1 CDS GPR1 NC_001136.8 389169 392054 R Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis; Gpr1p complement(389169..392054) Saccharomyces cerevisiae 851527 NP_010251.1 CDS SLM3 NC_001136.8 392656 393909 R tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF); Slm3p complement(392656..393909) Saccharomyces cerevisiae 851529 NP_010253.1 CDS DBP10 NC_001136.8 394214 397201 D Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis; Dbp10p 394214..397201 Saccharomyces cerevisiae 851530 NP_010254.1 CDS PRP9 NC_001136.8 397534 399126 D Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex; Prp9p 397534..399126 Saccharomyces cerevisiae 851531 NP_010255.1 CDS ARP2 NC_001136.8 399337 400635 D Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; Arp2p join(399337..399358,399482..400635) Saccharomyces cerevisiae 851532 NP_010256.1 CDS MPS1 NC_001136.8 400994 403288 R Mps1p; Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p complement(400994..403288) Saccharomyces cerevisiae 851533 NP_010257.1 CDS YDL027C NC_001136.8 403692 404954 R Ydl027cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene complement(403692..404954) Saccharomyces cerevisiae 851535 NP_010259.1 CDS YDL025C NC_001136.8 405341 407203 R Ydl025cp; Putative protein kinase, potentially phosphorylated by Cdc28p; YDL025C is not an essential gene complement(405341..407203) Saccharomyces cerevisiae 851536 NP_010260.1 CDS DIA3 NC_001136.8 408448 409854 R Protein of unknown function, involved in invasive and pseudohyphal growth; Dia3p complement(408448..409854) Saccharomyces cerevisiae 851537 NP_010262.1 CDS GPD1 NC_001136.8 411822 412997 D NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; Gpd1p 411822..412997 Saccharomyces cerevisiae 851539 NP_010263.1 CDS GPM2 NC_001136.8 413950 414885 D Homolog of Gpm1p phosphoglycerate mutase which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event; Gpm2p 413950..414885 Saccharomyces cerevisiae 851541 NP_010264.1 CDS RPN4 NC_001136.8 415110 416705 R Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; Rpn4p complement(415110..416705) Saccharomyces cerevisiae 851542 NP_010265.1 CDS OSH2 NC_001136.8 417660 421511 R Osh2p; Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability complement(417660..421511) Saccharomyces cerevisiae 851543 NP_010266.1 CDS ERP3 NC_001136.8 422830 423507 R Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; Erp3p complement(422830..423507) Saccharomyces cerevisiae 851544 NP_010267.1 CDS CDC7 NC_001136.8 424206 425729 D DDK (Dbf4-dependent kinase) catalytic subunit required for firing origins and replication fork progression in mitosis through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; Cdc7p 424206..425729 Saccharomyces cerevisiae 851545 NP_010269.1 CDS TSC13 NC_001136.8 425999 426931 R Tsc13p; Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4p complement(425999..426931) Saccharomyces cerevisiae 851547 NP_010270.1 CDS NOP1 NC_001136.8 427361 428344 D Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin; Nop1p 427361..428344 Saccharomyces cerevisiae 851548 NP_010271.1 CDS SLX5 NC_001136.8 429064 430923 D Subunit of the Slx5-Slx8 substrate-specific ubiquitin ligase complex; stimulated by prior attachment of SUMO to the substrate; Slx5p 429064..430923 Saccharomyces cerevisiae 851549 NP_010272.1 CDS YDL012C NC_001136.8 431105 431514 R Ydl012cp; Plasma membrane protein of unknown function; YDL012C is not an essential gene complement(join(431105..431383,431470..431514)) Saccharomyces cerevisiae 851550 NP_010274.1 CDS GRX6 NC_001136.8 432327 433022 D Monothiol glutaredoxin that binds an iron-sulfur cluster; more similar in activity to dithiol than other monothiol glutaredoxins; forms homodimers; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; Grx6p 432327..433022 Saccharomyces cerevisiae 851551 NP_010276.1 CDS APC11 NC_001136.8 433494 433991 D Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Apc11p 433494..433991 Saccharomyces cerevisiae 851554 NP_001032574.1 CDS YDL007C-A NC_001136.8 436564 436821 R Ydl007c-ap; Putative protein of unknown function complement(436564..436821) Saccharomyces cerevisiae 3799969 NP_010277.1 CDS RPT2 NC_001136.8 438044 439357 D Rpt2p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle 438044..439357 Saccharomyces cerevisiae 851557 NP_010278.1 CDS PTC1 NC_001136.8 439906 440751 D Type 2C protein phosphatase (PP2C); inactivates the osmosensing MAPK cascade by dephosphorylating Hog1p; mutation delays mitochondrial inheritance; deletion reveals defects in precursor tRNA splicing, sporulation and cell separation; Ptc1p 439906..440751 Saccharomyces cerevisiae 851558 NP_010279.1 CDS MED2 NC_001136.8 441011 442306 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med2p complement(441011..442306) Saccharomyces cerevisiae 851559 NP_010280.1 CDS ATP16 NC_001136.8 443026 443508 D Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated; Atp16p 443026..443508 Saccharomyces cerevisiae 851560 NP_010281.1 CDS MCD1 NC_001136.8 444680 446380 D Mcd1p; Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase 444680..446380 Saccharomyces cerevisiae 851561 NP_010282.1 CDS NHP10 NC_001136.8 446964 447575 R Protein related to mammalian high mobility group proteins; likely component of the INO80 complex, which is an ATP-dependent chromatin-remodeling complex; Nhp10p complement(446964..447575) Saccharomyces cerevisiae 851562 NP_010283.1 CDS RMD1 NC_001136.8 447981 449273 D Rmd1p; Cytoplasmic protein required for sporulation 447981..449273 Saccharomyces cerevisiae 851563 NP_010284.1 CDS NTH1 NC_001136.8 450217 452472 R Nth1p; Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p complement(450217..452472) Saccharomyces cerevisiae 851564 NP_010285.1 CDS YRB1 NC_001136.8 453042 453647 D Yrb1p; Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 453042..453647 Saccharomyces cerevisiae 851565 NP_010286.1 CDS RCR2 NC_001136.8 454119 454751 D Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; similar to Rcr1p; Rcr2p 454119..454751 Saccharomyces cerevisiae 851566 NP_878061.1 CDS YDR003W-A NC_001136.8 454779 454901 D Ydr003w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 454779..454901 Saccharomyces cerevisiae 1466432 NP_010287.1 CDS RAD57 NC_001136.8 455198 456580 D Rad57p; Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p 455198..456580 Saccharomyces cerevisiae 851567 NP_010288.1 CDS MAF1 NC_001136.8 456833 458100 R Negative regulator of RNA polymerase III; component of several signaling pathways that repress polymerase III transcription in response to changes in cellular environment; targets the initiation factor TFIIIB; Maf1p complement(join(456833..458014,458095..458100)) Saccharomyces cerevisiae 851568 NP_010289.1 CDS SOK1 NC_001136.8 458539 461244 R Sok1p; Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 complement(458539..461244) Saccharomyces cerevisiae 851569 NP_010290.1 CDS TRP1 NC_001136.8 461839 462513 D Trp1p; Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) 461839..462513 Saccharomyces cerevisiae 851570 NP_010292.1 CDS GAL3 NC_001136.8 463431 464993 D Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; Gal3p 463431..464993 Saccharomyces cerevisiae 851572 NP_010294.1 CDS SNQ2 NC_001136.8 465916 470421 D Snq2p; Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species 465916..470421 Saccharomyces cerevisiae 851574 NP_010295.1 CDS RPL4B NC_001136.8 471850 472938 D Rpl4bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins 471850..472938 Saccharomyces cerevisiae 851575 NP_010296.1 CDS PSF1 NC_001136.8 473154 473780 D Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery; Psf1p 473154..473780 Saccharomyces cerevisiae 851576 NP_010297.1 CDS RAD61 NC_001136.8 474043 475986 D Rad61p; Protein of unknown function; mutation confers radiation sensitivity 474043..475986 Saccharomyces cerevisiae 851577 NP_001035220.1 CDS HED1 NC_001136.8 477794 478282 D Meiosis-specific protein that down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; early meiotic gene, transcribed specifically during meiotic prophase; Hed1p 477794..478282 Saccharomyces cerevisiae 4036073 NP_010299.1 CDS DAD1 NC_001136.8 478471 478755 R Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Dad1p complement(478471..478755) Saccharomyces cerevisiae 851579 NP_010300.1 CDS KCS1 NC_001136.8 479112 482264 R Kcs1p; Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance complement(479112..482264) Saccharomyces cerevisiae 851580 NP_010301.1 CDS YDR018C NC_001136.8 482667 483857 R Ydr018cp; Probable membrane protein with three predicted transmembrane domains; homologous to Ybr042cp, similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; null exhibits no apparent phenotype complement(482667..483857) Saccharomyces cerevisiae 851581 NP_010302.1 CDS GCV1 NC_001136.8 484160 485362 R T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm; Gcv1p complement(484160..485362) Saccharomyces cerevisiae 851582 NP_010303.1 CDS DAS2 NC_001136.8 485743 486441 R Predicted protein shares weak similarity with uridine kinases and with phosphoribokinases; null mutant suppresses dst1delta sensitivity for 6-azauracil; Das2p complement(485743..486441) Saccharomyces cerevisiae 851583 NP_010304.1 CDS FAL1 NC_001136.8 486801 488000 D Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; Fal1p 486801..488000 Saccharomyces cerevisiae 851584 NP_010305.1 CDS CIS1 NC_001136.8 488069 488659 R Protein required for autophagosome formation in concert with Atg17p; may be involved in microtubule organization; high-copy suppressor of CIK1 deletion; Cis1p complement(488069..488659) Saccharomyces cerevisiae 851585 NP_010306.1 CDS SES1 NC_001136.8 489505 490893 D Ses1p; Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p 489505..490893 Saccharomyces cerevisiae 851587 NP_010308.1 CDS RPS11A NC_001136.8 491512 492321 D Rps11ap; Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins join(491512..491556,491896..492321) Saccharomyces cerevisiae 851589 NP_010309.1 CDS YDR026C NC_001136.8 492553 494265 R Ydr026cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein that may bind to the Ter region of rDNA; interacts physically with Fob1p complement(492553..494265) Saccharomyces cerevisiae 851590 NP_010310.1 CDS VPS54 NC_001136.8 494646 497315 R Vps54p; Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p complement(494646..497315) Saccharomyces cerevisiae 851591 NP_010311.1 CDS REG1 NC_001136.8 497832 500876 R Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes; Reg1p complement(497832..500876) Saccharomyces cerevisiae 851592 NP_010313.1 CDS RAD28 NC_001136.8 501750 503270 R Rad28p; Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSA protein complement(501750..503270) Saccharomyces cerevisiae 851594 NP_010314.2 CDS MIC14 NC_001136.8 503495 503860 D Mitochondrial intermembrane space cysteine motif protein of 14 kDa; Mic14p 503495..503860 Saccharomyces cerevisiae 851595 NP_010315.1 CDS PST2 NC_001136.8 504096 504692 R Pst2p; Protein with similarity to members of a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(504096..504692) Saccharomyces cerevisiae 851596 NP_010316.1 CDS MRH1 NC_001136.8 508144 509106 D Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; has similarity to Hsp30p and Yro2p; Mrh1p 508144..509106 Saccharomyces cerevisiae 851597 NP_010317.1 CDS LYS14 NC_001136.8 509734 512106 R Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer; Lys14p complement(509734..512106) Saccharomyces cerevisiae 851598 NP_058138.1 CDS YDR034C-D NC_001136.8 514040 519353 R Ydr034c-dp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(514040..518059,518061..519353)) Saccharomyces cerevisiae 851599 NP_058139.1 CDS YDR034C-C NC_001136.8 518037 519353 R Ydr034c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(518037..519353) Saccharomyces cerevisiae 851600 NP_010318.1 CDS YDR034C-A NC_001136.8 520513 520689 R Ydr034c-ap; Putative protein of unknown function; contained within the solo Ty1 LTR element YDRWdelta7 complement(520513..520689) Saccharomyces cerevisiae 851602 NP_010319.1 CDS YDR034W-B NC_001136.8 521311 521466 D Ydr034w-bp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 521311..521466 Saccharomyces cerevisiae 851604 NP_010320.1 CDS ARO3 NC_001136.8 521813 522925 D Aro3p; 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan 521813..522925 Saccharomyces cerevisiae 851605 NP_010321.1 CDS EHD3 NC_001136.8 523208 524710 R Ehd3p; 3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis complement(523208..524710) Saccharomyces cerevisiae 851606 NP_010322.1 CDS KRS1 NC_001136.8 525437 527212 D Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis; Krs1p 525437..527212 Saccharomyces cerevisiae 851607 NP_010323.1 CDS ENA5 NC_001136.8 527419 530694 R Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family; Ena5p complement(527419..530694) Saccharomyces cerevisiae 851608 NP_010324.1 CDS ENA2 NC_001136.8 531304 534579 R P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux; Ena2p complement(531304..534579) Saccharomyces cerevisiae 851609 NP_010325.1 CDS ENA1 NC_001136.8 535189 538464 R P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance; Ena1p complement(535189..538464) Saccharomyces cerevisiae 851610 NP_010326.1 CDS RSM10 NC_001136.8 539800 540411 D Rsm10p; Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins 539800..540411 Saccharomyces cerevisiae 851611 NP_010327.1 CDS YDR042C NC_001136.8 540598 541200 R Ydr042cp; Putative protein of unknown function; expression is increased in ssu72-ts69 mutant complement(540598..541200) Saccharomyces cerevisiae 851612 NP_010328.1 CDS NRG1 NC_001136.8 542672 543367 R Transcriptional repressor that recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; Nrg1p complement(542672..543367) Saccharomyces cerevisiae 851613 NP_010329.1 CDS HEM13 NC_001136.8 546640 547626 D Hem13p; Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; localizes to the mitochondrial inner membrane; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) 546640..547626 Saccharomyces cerevisiae 851614 NP_010330.1 CDS RPC11 NC_001136.8 547976 548308 R Rpc11p; RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS complement(547976..548308) Saccharomyces cerevisiae 851615 NP_010331.1 CDS BAP3 NC_001136.8 548760 550574 R Bap3p; Amino acid permease involved in the uptake of cysteine, leucine, isoleucine and valine complement(548760..550574) Saccharomyces cerevisiae 851616 NP_010332.1 CDS HEM12 NC_001136.8 551858 552946 D Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; activity inhibited by Cu2+, Zn2+, Fe2+, Fe3+ and sulfhydryl-specific reagents; Hem12p 551858..552946 Saccharomyces cerevisiae 851617 NP_010334.1 CDS YDR049W NC_001136.8 553252 555150 D Zinc finger protein; putative transcription factor that may interact with proteins involved in histone acetylation or deacetylation; may be involved in altering acetylation on histone lysines; Ydr049wp 553252..555150 Saccharomyces cerevisiae 851618 NP_010335.1 CDS TPI1 NC_001136.8 555724 556470 R Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; Tpi1p complement(555724..556470) Saccharomyces cerevisiae 851620 NP_010336.1 CDS YDR051C NC_001136.8 557054 558058 R Ydr051cp; Protein of unknown function; interacts with Hsp82p in two-hybrid assay; deletion confers sensitivity to Nickel complement(557054..558058) Saccharomyces cerevisiae 851621 NP_010337.1 CDS DBF4 NC_001136.8 558509 560623 R Regulatory subunit of Cdc7p-Dbf4p kinase complex, required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; Dbf4p complement(558509..560623) Saccharomyces cerevisiae 851623 NP_010339.1 CDS CDC34 NC_001136.8 561438 562325 R Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; Cdc34p complement(561438..562325) Saccharomyces cerevisiae 851624 NP_010340.1 CDS PST1 NC_001136.8 563525 564859 D Pst1p; Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 563525..564859 Saccharomyces cerevisiae 851625 NP_010341.1 CDS YDR056C NC_001136.8 565021 565638 R Ydr056cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic retiuculum; YDR056C is not an essential protein complement(565021..565638) Saccharomyces cerevisiae 851626 NP_010342.1 CDS YOS9 NC_001136.8 565925 567553 D Yos9p; ER quality-control lectin; integral subunit of the HRD ligase; serves as a receptor for misfolded N-glycosylated proteins, participates in their targeting to ERAD; member of the OS-9 protein family 565925..567553 Saccharomyces cerevisiae 851627 NP_010343.1 CDS TGL2 NC_001136.8 567721 568701 R Tgl2p; Protein with lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli; role in yeast lipid degradation is unclear complement(567721..568701) Saccharomyces cerevisiae 851628 NP_010344.1 CDS UBC5 NC_001136.8 569232 569768 R Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins, central component of the cellular stress response; expression is heat inducible; Ubc5p complement(join(569232..569631,569722..569768)) Saccharomyces cerevisiae 851631 NP_010345.1 CDS MAK21 NC_001136.8 570646 573723 D Mak21p; Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein 570646..573723 Saccharomyces cerevisiae 851632 NP_010346.1 CDS YDR061W NC_001136.8 574161 575780 D Ydr061wp; Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 574161..575780 Saccharomyces cerevisiae 851633 NP_010347.1 CDS LCB2 NC_001136.8 576471 578156 D Lcb2p; Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine 576471..578156 Saccharomyces cerevisiae 851634 NP_010348.1 CDS AIM7 NC_001136.8 578661 579110 D Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is viable and displays decreased frequency of mitochondrial genome loss (petite formation); Aim7p 578661..579110 Saccharomyces cerevisiae 851635 NP_010349.1 CDS RPS13 NC_001136.8 579456 580450 D Rps13p; Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins join(579456..579476,580016..580450) Saccharomyces cerevisiae 851636 NP_010350.1 CDS YDR065W NC_001136.8 580685 581782 D Ydr065wp; Protein of unknown function, required for vacuolar acidification; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 580685..581782 Saccharomyces cerevisiae 851637 NP_010351.1 CDS RTR2 NC_001136.8 581906 582496 R Rtr2p; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene complement(581906..582496) Saccharomyces cerevisiae 851638 NP_010352.1 CDS OCA6 NC_001136.8 582789 583463 R Oca6p; Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts complement(582789..583463) Saccharomyces cerevisiae 851639 NP_010353.1 CDS DOS2 NC_001136.8 583711 584643 D Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; Dos2p 583711..584643 Saccharomyces cerevisiae 851640 NP_010354.1 CDS DOA4 NC_001136.8 584937 587717 R Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole; Doa4p complement(584937..587717) Saccharomyces cerevisiae 851641 NP_010355.1 CDS FMP16 NC_001136.8 588096 588377 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp16p complement(588096..588377) Saccharomyces cerevisiae 851642 NP_010356.1 CDS PAA1 NC_001136.8 588825 589400 R Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication; Paa1p complement(588825..589400) Saccharomyces cerevisiae 851643 NP_010357.1 CDS IPT1 NC_001136.8 589759 591342 R Ipt1p; Inositolphosphotransferase 1, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), which is the most abundant sphingolipid in cells, mutation confers resistance to the antifungals syringomycin E and DmAMP1 in some growth media complement(589759..591342) Saccharomyces cerevisiae 851644 NP_010358.1 CDS SNF11 NC_001136.8 592436 592945 D Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; Snf11p 592436..592945 Saccharomyces cerevisiae 851645 NP_010359.1 CDS TPS2 NC_001136.8 593890 596580 D Tps2p; Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway 593890..596580 Saccharomyces cerevisiae 851646 NP_010360.1 CDS PPH3 NC_001136.8 597153 598079 D Pph3p; Catalytic subunit of an evolutionarily conserved protein phosphatase complex containing Psy2p and the regulatory subunit Psy4p; required for cisplatin resistance; involved in activation of Gln3p 597153..598079 Saccharomyces cerevisiae 851647 NP_010361.1 CDS RAD55 NC_001136.8 598465 599685 D Rad55p; Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p 598465..599685 Saccharomyces cerevisiae 851648 NP_010362.1 CDS SED1 NC_001136.8 600790 601806 D Sed1p; Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites 600790..601806 Saccharomyces cerevisiae 851649 NP_010363.1 CDS SHU2 NC_001136.8 602195 602866 R Shu2p; Protein of unassigned function involved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation; associates with Shu1p, Psy3p, and Csm2p complement(602195..602866) Saccharomyces cerevisiae 851650 NP_010364.1 CDS PET100 NC_001136.8 603061 603396 D Pet100p; Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme 603061..603396 Saccharomyces cerevisiae 851651 NP_076886.1 CDS TFB5 NC_001136.8 603590 603808 R Tfb5p; Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation; homolog of the Chlamydomonas reinhardtii REX1-S protein which is involved in DNA repair complement(603590..603808) Saccharomyces cerevisiae 851652 NP_010365.1 CDS VPS41 NC_001136.8 604005 606983 D Vps41p; Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport 604005..606983 Saccharomyces cerevisiae 851653 NP_010366.1 CDS PDC2 NC_001136.8 607301 610078 R Transcription factor required for the synthesis of the glycolytic enzyme pyruvate decarboxylase, required for high level expression of both the THI and the PDC genes; Pdc2p complement(607301..610078) Saccharomyces cerevisiae 851654 NP_010367.1 CDS STN1 NC_001136.8 610438 611922 D Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; Stn1p 610438..611922 Saccharomyces cerevisiae 851655 NP_010368.3 CDS RRP8 NC_001136.8 612070 613248 D Rrp8p; Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation 612070..613248 Saccharomyces cerevisiae 851656 NP_010369.1 CDS TVP23 NC_001136.8 613402 614001 R Tvp23p; Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(613402..614001) Saccharomyces cerevisiae 851657 NP_010370.1 CDS AFR1 NC_001136.8 614285 616147 R Alpha-factor pheromone receptor regulator, negatively regulates pheromone receptor signaling; required for normal mating projection (shmoo) formation; required for Spa2p to recruit Mpk1p to shmoo tip during mating; interacts with Cdc12p; Afr1p complement(614285..616147) Saccharomyces cerevisiae 851658 NP_010371.1 CDS SSS1 NC_001136.8 616925 617167 R Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p; Sss1p complement(616925..617167) Saccharomyces cerevisiae 851659 NP_010372.1 CDS RRP1 NC_001136.8 617467 618303 R Rrp1p; Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles complement(617467..618303) Saccharomyces cerevisiae 851660 NP_010373.1 CDS SLU7 NC_001136.8 618494 619642 R Slu7p; RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain complement(618494..619642) Saccharomyces cerevisiae 851661 NP_010374.1 CDS YDR089W NC_001136.8 622109 624718 D Ydr089wp; Protein of unknown function; deletion confers resistance to Nickel 622109..624718 Saccharomyces cerevisiae 851663 NP_010375.1 CDS YDR090C NC_001136.8 625063 625995 R Ydr090cp; Putative protein of unknown function complement(625063..625995) Saccharomyces cerevisiae 851664 NP_010376.1 CDS RLI1 NC_001136.8 626705 628531 R Rli1p; Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPase complement(626705..628531) Saccharomyces cerevisiae 851665 NP_010377.1 CDS UBC13 NC_001136.8 629873 630602 D Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus; Ubc13p join(629873..629902,630171..630602) Saccharomyces cerevisiae 851666 NP_010378.1 CDS DNF2 NC_001136.8 631279 636117 D Dnf2p; Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase 631279..636117 Saccharomyces cerevisiae 851667 NP_010381.1 CDS GIS1 NC_001136.8 637136 639820 D JmjC domain-containing histone demethylase; transcription factor involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1; Gis1p 637136..639820 Saccharomyces cerevisiae 851670 NP_010382.1 CDS MSH6 NC_001136.8 640106 643834 R Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p; Msh6p complement(640106..643834) Saccharomyces cerevisiae 851671 NP_010383.1 CDS GRX3 NC_001136.8 644175 645032 R Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; Grx3p complement(644175..645032) Saccharomyces cerevisiae 851672 NP_058140.1 CDS YDR098C-B NC_001136.8 645855 651123 R Ydr098c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(645855..649817,649819..651123)) Saccharomyces cerevisiae 851674 NP_058141.1 CDS YDR098C-A NC_001136.8 649801 651123 R Ydr098c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(649801..651123) Saccharomyces cerevisiae 851675 NP_010384.1 CDS BMH2 NC_001136.8 653604 654425 D Bmh2p; 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling 653604..654425 Saccharomyces cerevisiae 851676 NP_010385.1 CDS TVP15 NC_001136.8 655010 655441 D Tvp15p; Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p 655010..655441 Saccharomyces cerevisiae 851677 NP_010386.1 CDS ARX1 NC_001136.8 655683 657464 R Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex; Arx1p complement(655683..657464) Saccharomyces cerevisiae 851678 NP_010388.1 CDS STE5 NC_001136.8 658347 661100 D Ste5p; Pheromone-response scaffold protein; binds kinases Ste11p, Ste7p, and Fus3p to form a MAPK cascade complex that interacts with the plasma membrane, via a PH (pleckstrin homology) and PM/NLS domain, and with Ste4p-Ste18p, during signaling 658347..661100 Saccharomyces cerevisiae 851680 NP_010389.1 CDS SPO71 NC_001136.8 661170 664907 R Spo71p; Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis complement(661170..664907) Saccharomyces cerevisiae 851681 NP_010390.1 CDS TMS1 NC_001136.8 665346 666767 R Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance; Tms1p complement(665346..666767) Saccharomyces cerevisiae 851682 NP_010391.1 CDS ARP10 NC_001136.8 667001 667855 D Component of the dynactin complex, localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex; Arp10p 667001..667855 Saccharomyces cerevisiae 851683 NP_010392.1 CDS TMN2 NC_001136.8 669013 671031 R Tmn2p; Multispanning membrane protein of unknown function complement(669013..671031) Saccharomyces cerevisiae 851685 NP_010393.1 CDS GSG1 NC_001136.8 671266 673362 D Subunit of TRAPP (transport protein particle), a multi-subunit complex involved in targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment; protein has late meiotic role, following DNA replication; Gsg1p 671266..673362 Saccharomyces cerevisiae 851686 NP_010394.1 CDS YDR109C NC_001136.8 673517 675664 R Ydr109cp; Putative kinase complement(673517..675664) Saccharomyces cerevisiae 851687 NP_010395.1 CDS FOB1 NC_001136.8 676099 677799 D Nucleolar protein required for DNA replication fork blocking and recombinational hotspot activities; binds to the replication fork barrier site in the rDNA region; related to retroviral integrases; Fob1p 676099..677799 Saccharomyces cerevisiae 851688 NP_010396.1 CDS ALT2 NC_001136.8 678238 679761 R Putative alanine transaminase (glutamic pyruvic transaminase); Alt2p complement(678238..679761) Saccharomyces cerevisiae 851690 NP_010398.1 CDS PDS1 NC_001136.8 680493 681614 R Securin that inhibits anaphase by binding separin Esp1p, also blocks cyclin destruction and mitotic exit, essential for cell cycle arrest in mitosis in the presence of DNA damage or aberrant mitotic spindles; also present in meiotic nuclei; Pds1p complement(680493..681614) Saccharomyces cerevisiae 851691 NP_010399.1 CDS YDR114C NC_001136.8 681921 682223 R Ydr114cp; Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium complement(681921..682223) Saccharomyces cerevisiae 851693 NP_010400.1 CDS YDR115W NC_001136.8 682172 682489 D Ydr115wp; Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins 682172..682489 Saccharomyces cerevisiae 851692 NP_010401.1 CDS MRPL1 NC_001136.8 682721 683578 R Mrpl1p; Mitochondrial ribosomal protein of the large subunit complement(682721..683578) Saccharomyces cerevisiae 851694 NP_010402.1 CDS TMA64 NC_001136.8 683943 685640 R Tma64p; Protein of unknown function that associates with ribosomes; has a putative RNA binding domain complement(683943..685640) Saccharomyces cerevisiae 851695 NP_010403.1 CDS APC4 NC_001136.8 685879 687837 D Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Apc4p 685879..687837 Saccharomyces cerevisiae 851696 NP_010404.1 CDS YDR119W NC_001136.8 688224 690530 D Ydr119wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuolar membrane; YDR119W is not an essential gene 688224..690530 Saccharomyces cerevisiae 851697 NP_001032575.1 CDS YDR119W-A NC_001136.8 691011 691211 D Ydr119w-ap; Putative protein of unknown function 691011..691211 Saccharomyces cerevisiae 3799970 NP_010405.1 CDS TRM1 NC_001136.8 691546 693258 R tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments; Trm1p complement(691546..693258) Saccharomyces cerevisiae 851698 NP_010406.1 CDS DPB4 NC_001136.8 693582 694172 D Shared subunit of DNA polymerase (II) epsilon and of ISW2/yCHRAC chromatin accessibility complex; involved in both chromosomal DNA replication and in inheritance of telomeric silencing; Dpb4p 693582..694172 Saccharomyces cerevisiae 851699 NP_010407.1 CDS KIN1 NC_001136.8 694697 697891 D Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p; Kin1p 694697..697891 Saccharomyces cerevisiae 851700 NP_010408.1 CDS INO2 NC_001136.8 698551 699465 R Ino2p; Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion complement(698551..699465) Saccharomyces cerevisiae 851701 NP_010409.1 CDS YDR124W NC_001136.8 700312 701286 D Ydr124wp; Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor 700312..701286 Saccharomyces cerevisiae 851702 NP_010410.1 CDS ECM18 NC_001136.8 701394 702755 R Protein of unknown function, similar to Rlp24p; Ecm18p complement(701394..702755) Saccharomyces cerevisiae 851703 NP_010411.1 CDS SWF1 NC_001136.8 703231 704241 D Swf1p; Palmitoyltransferase that acts on the SNAREs Snc1p, Syn8p, Tlg1p and likely on all SNAREs; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion 703231..704241 Saccharomyces cerevisiae 851704 NP_010412.1 CDS ARO1 NC_001136.8 704481 709247 D Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids; Aro1p 704481..709247 Saccharomyces cerevisiae 851705 NP_010413.1 CDS YDR128W NC_001136.8 709546 712992 D Ydr128wp; Putative protein of unknown function 709546..712992 Saccharomyces cerevisiae 851706 NP_010414.1 CDS SAC6 NC_001136.8 713337 715376 R Sac6p; Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton complement(join(713337..715244,715356..715376)) Saccharomyces cerevisiae 851707 NP_010415.1 CDS FIN1 NC_001136.8 715744 716619 R Spindle pole body-related intermediate filament protein, forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells, able to self-assemble, expression induced during S/G2, localization cell-cycle dependent; Fin1p complement(715744..716619) Saccharomyces cerevisiae 851708 NP_010416.1 CDS YDR131C NC_001136.8 716787 718457 R Ydr131cp; F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex complement(716787..718457) Saccharomyces cerevisiae 851709 NP_010417.1 CDS YDR132C NC_001136.8 718813 720300 R Ydr132cp; Putative protein of unknown function complement(718813..720300) Saccharomyces cerevisiae 851710 NP_010419.1 CDS YCF1 NC_001136.8 723001 727548 R Ycf1p; Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin; similar to human cystic fibrosis protein CFTR complement(723001..727548) Saccharomyces cerevisiae 851713 NP_010421.1 CDS RGP1 NC_001136.8 728256 730247 D Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p; Rgp1p 728256..730247 Saccharomyces cerevisiae 851714 NP_010422.1 CDS HPR1 NC_001136.8 730575 732833 D Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p; Hpr1p 730575..732833 Saccharomyces cerevisiae 851716 NP_010423.2 CDS RUB1 NC_001136.8 733615 733921 R Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme); Rub1p complement(join(733615..733699,733773..733921)) Saccharomyces cerevisiae 851717 NP_010424.1 CDS MTQ2 NC_001136.8 734135 734800 D S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; methylates release factor eRF1 (Sup45p) in vitro; is not an essential gene; similar to E.coli PrmC; Mtq2p 734135..734800 Saccharomyces cerevisiae 851718 NP_010425.1 CDS DOP1 NC_001136.8 734898 739994 R Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Dop1p complement(734898..739994) Saccharomyces cerevisiae 851719 NP_010426.1 CDS PEX7 NC_001136.8 740470 741597 R Pex7p; Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) complement(740470..741597) Saccharomyces cerevisiae 851720 NP_010427.1 CDS SAN1 NC_001136.8 742039 743871 R Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear proteins; San1p complement(742039..743871) Saccharomyces cerevisiae 851721 NP_010428.1 CDS MKC7 NC_001136.8 744308 746098 R Mkc7p; GPI-anchored aspartyl protease (yapsin) involved in protein processing; shares functions with Yap3p and Kex2p complement(744308..746098) Saccharomyces cerevisiae 851722 NP_010429.1 CDS TAF12 NC_001136.8 746735 748354 D Taf12p; Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A 746735..748354 Saccharomyces cerevisiae 851723 NP_010430.1 CDS SWI5 NC_001136.8 748610 750739 R Transcription factor that activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; Swi5p complement(748610..750739) Saccharomyces cerevisiae 851724 NP_010431.1 CDS EKI1 NC_001136.8 751628 753232 D Ethanolamine kinase, primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; Eki1p 751628..753232 Saccharomyces cerevisiae 851725 NP_010432.1 CDS KGD2 NC_001136.8 753672 755063 R Kgd2p; Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated complement(753672..755063) Saccharomyces cerevisiae 851726 NP_010434.1 CDS NUM1 NC_001136.8 755625 763871 D Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex; Num1p 755625..763871 Saccharomyces cerevisiae 851727 NP_010435.1 CDS CTH1 NC_001136.8 764175 765152 R Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; Cth1p complement(764175..765152) Saccharomyces cerevisiae 851729 NP_010436.1 CDS GIR2 NC_001136.8 765703 766500 D Highly-acidic cytoplasmic RWD domain-containing protein of unknown function, sensitive to proteolysis, N-terminal region has high content of acidic amino acid residues, putative IUP (intrinsically unstructured protein); Gir2p 765703..766500 Saccharomyces cerevisiae 851730 NP_010437.1 CDS ENT5 NC_001136.8 766733 767968 R Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin; Ent5p complement(766733..767968) Saccharomyces cerevisiae 851731 NP_010439.1 CDS CPR1 NC_001136.8 768509 768997 R Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; Cpr1p complement(768509..768997) Saccharomyces cerevisiae 851733 NP_010440.1 CDS RPA14 NC_001136.8 769522 769935 D Rpa14p; RNA polymerase I subunit A14 769522..769935 Saccharomyces cerevisiae 851734 NP_010442.1 CDS HOM2 NC_001136.8 770354 771451 D Hom2p; Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis 770354..771451 Saccharomyces cerevisiae 851736 NP_010443.1 CDS SAC3 NC_001136.8 771874 775779 D Sac3p; Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus 771874..775779 Saccharomyces cerevisiae 851737 NP_010444.1 CDS SSY1 NC_001136.8 776160 778718 D Ssy1p; Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes 776160..778718 Saccharomyces cerevisiae 851738 NP_010445.1 CDS YDR161W NC_001136.8 779040 780203 D Ydr161wp; Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation 779040..780203 Saccharomyces cerevisiae 851739 NP_010446.1 CDS NBP2 NC_001136.8 780387 781097 R Protein involved in the HOG (high osmolarity glycerol) pathway, negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex, found in the nucleus and cytoplasm, contains an SH3 domain that binds Pbs2p; Nbp2p complement(780387..781097) Saccharomyces cerevisiae 851740 NP_010447.1 CDS CWC15 NC_001136.8 781420 781947 D Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p; Cwc15p 781420..781947 Saccharomyces cerevisiae 851741 NP_010448.1 CDS SEC1 NC_001136.8 782038 784212 R Sm-like protein involved in docking and fusion of exocytic vesicles through binding to assembled SNARE complexes at the membrane; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; Sec1p complement(782038..784212) Saccharomyces cerevisiae 851742 NP_010449.1 CDS TRM82 NC_001136.8 784868 786202 D Trm82p; Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA 784868..786202 Saccharomyces cerevisiae 851743 NP_010450.1 CDS SEC5 NC_001136.8 786303 789218 R Sec5p; Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis complement(786303..789218) Saccharomyces cerevisiae 851744 NP_010451.1 CDS TAF10 NC_001136.8 789446 790066 D Taf10p; Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification 789446..790066 Saccharomyces cerevisiae 851745 NP_010452.1 CDS CDC37 NC_001136.8 790325 791845 D Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding; Cdc37p 790325..791845 Saccharomyces cerevisiae 851746 NP_010453.1 CDS STB3 NC_001136.8 792345 793886 R Stb3p; Protein that binds Sin3p in a two-hybrid assay complement(792345..793886) Saccharomyces cerevisiae 851747 NP_878062.1 CDS YDR169C-A NC_001136.8 794571 794720 R Ydr169c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(794571..794720) Saccharomyces cerevisiae 1466433 NP_010454.1 CDS SEC7 NC_001136.8 796190 802219 R Sec7p; Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles complement(796190..802219) Saccharomyces cerevisiae 851748 NP_010455.1 CDS YDR170W-A NC_001136.8 803192 804514 D Ydr170w-ap; Retrotransposon TYA Gag gene; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag; YDR170W-A is part of a mutant retrotransposon 803192..804514 Saccharomyces cerevisiae 851750 NP_010456.1 CDS HSP42 NC_001136.8 806618 807745 D Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; Hsp42p 806618..807745 Saccharomyces cerevisiae 851751 NP_010457.1 CDS SUP35 NC_001136.8 808321 810378 D Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype; Sup35p 808321..810378 Saccharomyces cerevisiae 851752 NP_010458.1 CDS ARG82 NC_001136.8 810562 811629 R Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes; Arg82p complement(810562..811629) Saccharomyces cerevisiae 851753 NP_010459.1 CDS HMO1 NC_001136.8 812107 812847 D Hmo1p; Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase 812107..812847 Saccharomyces cerevisiae 851754 NP_010460.1 CDS RSM24 NC_001136.8 813190 814149 R Rsm24p; Mitochondrial ribosomal protein of the small subunit complement(813190..814149) Saccharomyces cerevisiae 851755 NP_010461.1 CDS NGG1 NC_001136.8 814449 816557 D Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex; Ngg1p 814449..816557 Saccharomyces cerevisiae 851756 NP_010462.1 CDS UBC1 NC_001136.8 816875 817522 D Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; Ubc1p 816875..817522 Saccharomyces cerevisiae 851757 NP_010463.1 CDS SDH4 NC_001136.8 817947 818492 D Sdh4p; Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone 817947..818492 Saccharomyces cerevisiae 851758 NP_010464.1 CDS CSN9 NC_001136.8 818705 819193 R Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; Csn9p complement(818705..819193) Saccharomyces cerevisiae 851759 NP_010465.2 CDS YDR179W-A NC_001136.8 819430 820821 D Ydr179w-ap; Putative protein of unknown function 819430..820821 Saccharomyces cerevisiae 851760 NP_010466.1 CDS SCC2 NC_001136.8 821292 825773 D Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; Scc2p 821292..825773 Saccharomyces cerevisiae 851761 NP_010467.1 CDS SAS4 NC_001136.8 825907 827352 R Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p; Sas4p complement(825907..827352) Saccharomyces cerevisiae 851762 NP_010468.1 CDS CDC1 NC_001136.8 827579 829054 D Putative membrane protein of unknown function involved in Mn2+ homeostasis; mutants display actin and general growth defects, heterogeneous cell cycle arrests, and pleiotropic defects in cell cycle progression and organelle distribution; Cdc1p 827579..829054 Saccharomyces cerevisiae 851763 NP_878063.1 CDS YDR182W-A NC_001136.8 829148 829351 D Ydr182w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 829148..829351 Saccharomyces cerevisiae 1466434 NP_010469.1 CDS PLP1 NC_001136.8 829582 830274 D Protein with a possible role in folding of beta-tubulin; has weak similarity to phosducins, which are G-protein regulators; Plp1p 829582..830274 Saccharomyces cerevisiae 851764 NP_010470.1 CDS ATC1 NC_001136.8 830626 831510 R Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; Atc1p complement(830626..831510) Saccharomyces cerevisiae 851765 NP_010471.1 CDS YDR185C NC_001136.8 831931 832470 R Ydr185cp; Mitochondrial protein of unknown function; has similarity to Ups1p, which is involved in regulation of alternative topogenesis of the dynamin-related GTPase Mgm1p complement(831931..832470) Saccharomyces cerevisiae 851766 NP_010472.1 CDS YDR186C NC_001136.8 832856 835489 R Ydr186cp; Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(832856..835489) Saccharomyces cerevisiae 851767 NP_010474.1 CDS CCT6 NC_001136.8 836418 838058 D Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif; Cct6p 836418..838058 Saccharomyces cerevisiae 851768 NP_010475.1 CDS SLY1 NC_001136.8 838389 840389 D Sly1p; Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex 838389..840389 Saccharomyces cerevisiae 851770 NP_010476.1 CDS RVB1 NC_001136.8 840601 841992 R Rvb1p; Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family complement(840601..841992) Saccharomyces cerevisiae 851771 NP_010477.1 CDS HST4 NC_001136.8 842334 843446 D Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; Hst4p 842334..843446 Saccharomyces cerevisiae 851772 NP_010478.1 CDS NUP42 NC_001136.8 843566 844858 R Subunit of the nuclear pore complex (NPC) that localizes exclusively to the cytoplasmic side; involved in RNA export, most likely at a terminal step; interacts with Gle1p; Nup42p complement(843566..844858) Saccharomyces cerevisiae 851774 NP_010480.1 CDS MSS116 NC_001136.8 845949 847943 R Mss116p; DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing complement(845949..847943) Saccharomyces cerevisiae 851775 NP_878064.1 CDS YDR194W-A NC_001136.8 848068 848220 D Ydr194w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 848068..848220 Saccharomyces cerevisiae 1466435 NP_010481.1 CDS REF2 NC_001136.8 848596 850197 D Ref2p; RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes 848596..850197 Saccharomyces cerevisiae 851776 NP_010482.1 CDS YDR196C NC_001136.8 850271 850996 R Ydr196cp; Putative dephospho-CoA kinase (DPCK) that catalyzes the final step in Coenzyme A biosynthesis; essential for viability; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(850271..850996) Saccharomyces cerevisiae 851777 NP_010483.1 CDS CBS2 NC_001136.8 851225 852394 D Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader; Cbs2p 851225..852394 Saccharomyces cerevisiae 851778 NP_010484.1 CDS RKM2 NC_001136.8 852530 853969 R Rkm2p; Ribosomal protein lysine methyltransferase, responsible for epsilon-trimethylation of the lysine residue at position 10 of Rpl12Ap and Rpl12Bp complement(852530..853969) Saccharomyces cerevisiae 851779 NP_010486.1 CDS VPS64 NC_001136.8 854276 856090 R Vps64p; Cytoplasmic protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p, Far7p, Far10p, and Far11p that is involved in pheromone-induced cell cycle arrest; also localized to the endoplasmic reticulum membrane complement(854276..856090) Saccharomyces cerevisiae 851781 NP_010487.1 CDS SPC19 NC_001136.8 856314 856811 D Spc19p; Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body 856314..856811 Saccharomyces cerevisiae 851782 NP_010488.1 CDS RAV2 NC_001136.8 856895 857950 R Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme; Rav2p complement(856895..857950) Saccharomyces cerevisiae 851784 NP_010490.1 CDS COQ4 NC_001136.8 858134 859141 D Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; Coq4p 858134..859141 Saccharomyces cerevisiae 851785 NP_010491.2 CDS MSC2 NC_001136.8 859343 861517 D Msc2p; Member of the cation diffusion facilitator family, localizes to the endoplasmic reticulum and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids 859343..861517 Saccharomyces cerevisiae 851786 NP_010492.1 CDS EBS1 NC_001136.8 862051 864705 D Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; Ebs1p 862051..864705 Saccharomyces cerevisiae 851787 NP_010493.1 CDS UME6 NC_001136.8 865009 867519 R Ume6p; Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p complement(865009..867519) Saccharomyces cerevisiae 851788 NP_010494.1 CDS MSS4 NC_001136.8 868221 870560 D Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation; Mss4p 868221..870560 Saccharomyces cerevisiae 851789 NP_010496.1 CDS YDR210W NC_001136.8 871071 871298 D Ydr210wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 871071..871298 Saccharomyces cerevisiae 851790 NP_058142.1 CDS YDR210W-B NC_001136.8 872109 877422 D Ydr210w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(872109..873401,873403..877422) Saccharomyces cerevisiae 851793 NP_058143.1 CDS YDR210W-A NC_001136.8 872109 873425 D Ydr210w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 872109..873425 Saccharomyces cerevisiae 851792 NP_620387.1 CDS YDR210C-D NC_001136.8 878656 883924 R Ydr210c-dp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(878656..882618,882620..883924)) Saccharomyces cerevisiae 851794 NP_620388.1 CDS YDR210C-C NC_001136.8 882602 883924 R Ydr210c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(882602..883924) Saccharomyces cerevisiae 851795 NP_010497.1 CDS GCD6 NC_001136.8 884724 886862 D Gcd6p; Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression 884724..886862 Saccharomyces cerevisiae 851797 NP_010498.1 CDS TCP1 NC_001136.8 887229 888908 D Tcp1p; Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptide 887229..888908 Saccharomyces cerevisiae 851798 NP_010499.1 CDS UPC2 NC_001136.8 889748 892489 D Upc2p; Sterol regulatory element binding protein, induces transcription of sterol transport and biosynthetic genes; involved in the anaerobic induction of DAN/TIR mannoproteins and seripauperins; binucleate zinc cluster protein; Ecm22p homolog 889748..892489 Saccharomyces cerevisiae 851799 NP_010500.1 CDS AHA1 NC_001136.8 892872 893924 D Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; Aha1p 892872..893924 Saccharomyces cerevisiae 851800 NP_010502.1 CDS ADR1 NC_001136.8 895031 899002 D Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization; Adr1p 895031..899002 Saccharomyces cerevisiae 851802 NP_010503.1 CDS RAD9 NC_001136.8 899547 903476 R Rad9p; DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate complement(899547..903476) Saccharomyces cerevisiae 851803 NP_010504.1 CDS SPR28 NC_001136.8 903777 905048 R Spr28p; Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation complement(903777..905048) Saccharomyces cerevisiae 851804 NP_010505.1 CDS MFB1 NC_001136.8 905451 906848 R Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding; Mfb1p complement(905451..906848) Saccharomyces cerevisiae 851805 NP_010507.1 CDS GTB1 NC_001136.8 907326 909434 D Gtb1p; Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p, involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER 907326..909434 Saccharomyces cerevisiae 851807 NP_010508.1 CDS YDR222W NC_001136.8 910050 911297 D Ydr222wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 910050..911297 Saccharomyces cerevisiae 851808 NP_010509.1 CDS CRF1 NC_001136.8 912095 913498 D Transcriptional corepressor involved in the regulation of ribosomal protein gene transcription via the TOR signaling pathway and protein kinase A, phosphorylated by activated Yak1p which promotes accumulation of Crf1p in the nucleus; Crf1p 912095..913498 Saccharomyces cerevisiae 851809 NP_010510.1 CDS HTB1 NC_001136.8 914313 914708 R One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation; Htb1p complement(914313..914708) Saccharomyces cerevisiae 851810 NP_010511.1 CDS HTA1 NC_001136.8 915526 915924 D One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; Hta1p 915526..915924 Saccharomyces cerevisiae 851811 NP_010512.1 CDS ADK1 NC_001136.8 916482 917150 D Adk1p; Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence 916482..917150 Saccharomyces cerevisiae 851812 NP_010513.1 CDS SIR4 NC_001136.8 917567 921643 D Sir4p; Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan 917567..921643 Saccharomyces cerevisiae 851813 NP_010514.1 CDS PCF11 NC_001136.8 921922 923802 R mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); Pcf11p complement(921922..923802) Saccharomyces cerevisiae 851814 NP_010515.1 CDS IVY1 NC_001136.8 924781 926142 D Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase; Ivy1p 924781..926142 Saccharomyces cerevisiae 851815 NP_010517.1 CDS COX20 NC_001136.8 926289 926906 R Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase; Cox20p complement(926289..926906) Saccharomyces cerevisiae 851817 NP_010518.1 CDS HEM1 NC_001136.8 927448 929094 D Hem1p; 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p 927448..929094 Saccharomyces cerevisiae 851818 NP_010519.1 CDS RTN1 NC_001136.8 929466 930353 R Rtn1p; ER membrane protein that interacts with exocyst subunit Sec6p and with Yip3p; also interacts with Sbh1p; null mutant has an altered (mostly cisternal) ER morphology; member of the RTNLA (reticulon-like A) subfamily complement(929466..930353) Saccharomyces cerevisiae 851819 NP_010520.1 CDS LYS4 NC_001136.8 931125 933206 D Lys4p; Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway 931125..933206 Saccharomyces cerevisiae 851820 NP_010521.1 CDS PRP42 NC_001136.8 933500 935134 D U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats; Prp42p 933500..935134 Saccharomyces cerevisiae 851821 NP_010522.1 CDS FMN1 NC_001136.8 935232 935888 R Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membrane; Fmn1p complement(935232..935888) Saccharomyces cerevisiae 851822 NP_010523.1 CDS MRPL7 NC_001136.8 936611 937489 D Mrpl7p; Mitochondrial ribosomal protein of the large subunit 936611..937489 Saccharomyces cerevisiae 851823 NP_010524.1 CDS SEC26 NC_001136.8 937891 940812 R Sec26p; Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) complement(937891..940812) Saccharomyces cerevisiae 851824 NP_010525.1 CDS YDR239C NC_001136.8 941053 943416 R Ydr239cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments complement(941053..943416) Saccharomyces cerevisiae 851825 NP_010526.1 CDS SNU56 NC_001136.8 943670 945148 R Snu56p; Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex complement(943670..945148) Saccharomyces cerevisiae 851827 NP_010528.1 CDS AMD2 NC_001136.8 946803 948452 D Putative amidase; Amd2p 946803..948452 Saccharomyces cerevisiae 851829 NP_010529.1 CDS PRP28 NC_001136.8 948514 950280 R RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site; Prp28p complement(948514..950280) Saccharomyces cerevisiae 851830 NP_010530.1 CDS PEX5 NC_001136.8 950559 952397 D Pex5p; Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions 950559..952397 Saccharomyces cerevisiae 851831 NP_010531.1 CDS MNN10 NC_001136.8 952796 953977 D Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; Mnn10p 952796..953977 Saccharomyces cerevisiae 851832 NP_010532.1 CDS TRS23 NC_001136.8 954284 954943 D Trs23p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 954284..954943 Saccharomyces cerevisiae 851833 NP_878065.1 CDS YDR246W-A NC_001136.8 955129 955329 D Ydr246w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 955129..955329 Saccharomyces cerevisiae 1466436 NP_010533.1 CDS VHS1 NC_001136.8 956009 957394 D Vhs1p; Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p 956009..957394 Saccharomyces cerevisiae 851834 NP_010534.1 CDS YDR248C NC_001136.8 957754 958335 R Ydr248cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(957754..958335) Saccharomyces cerevisiae 851835 NP_010535.1 CDS YDR249C NC_001136.8 958677 959798 R Ydr249cp; Putative protein of unknown function complement(958677..959798) Saccharomyces cerevisiae 851836 NP_010537.1 CDS PAM1 NC_001136.8 960610 963102 D Pam1p; Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype 960610..963102 Saccharomyces cerevisiae 851838 NP_010538.1 CDS BTT1 NC_001136.8 963408 963857 D Btt1p; Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex (alpha, beta1, beta3) which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides 963408..963857 Saccharomyces cerevisiae 851839 NP_010539.1 CDS MET32 NC_001136.8 963986 964561 R Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met31p; Met32p complement(963986..964561) Saccharomyces cerevisiae 851840 NP_010540.1 CDS CHL4 NC_001136.8 965109 966485 D Outer kinetochore protein required for chromosome stability, interacts with kinetochore proteins Ctf19p, Ctf3p, and Iml3p; exhibits a two-hybrid interaction with Mif2p; association with CEN DNA requires Ctf19p; Chl4p 965109..966485 Saccharomyces cerevisiae 851841 NP_010541.1 CDS RMD5 NC_001136.8 966553 967818 R Rmd5p; Cytosolic protein required for sporulation; also required for the ubiquitination of the gluconeogenetic enzyme fructose-1,6-bisphosphatase, which is degraded rapidly after the switch from gluconeogenesis to glycolysis complement(966553..967818) Saccharomyces cerevisiae 851842 NP_010542.1 CDS CTA1 NC_001136.8 968129 969676 R Cta1p; Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation complement(968129..969676) Saccharomyces cerevisiae 851843 NP_010543.1 CDS SET7 NC_001136.8 969986 971470 R Set7p; Nuclear protein that contains a SET-domain, which have been shown to mediate methyltransferase activity in other proteins complement(969986..971470) Saccharomyces cerevisiae 851844 NP_010544.1 CDS HSP78 NC_001136.8 971804 974239 R Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system; Hsp78p complement(971804..974239) Saccharomyces cerevisiae 851845 NP_010545.1 CDS YAP6 NC_001136.8 974627 975778 R Yap6p; Putative basic leucine zipper (bZIP) transcription factor; overexpression increases sodium and lithium tolerance complement(974627..975778) Saccharomyces cerevisiae 851846 NP_010546.1 CDS SWM1 NC_001136.8 976713 977225 R Swm1p; Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation complement(976713..977225) Saccharomyces cerevisiae 851847 NP_010547.1 CDS EXG2 NC_001136.8 977517 979205 R Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; Exg2p complement(977517..979205) Saccharomyces cerevisiae 851848 NP_058146.1 CDS YDR261W-B NC_001136.8 981458 986771 D Ydr261w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(981458..982750,982752..986771) Saccharomyces cerevisiae 851851 NP_058147.1 CDS YDR261W-A NC_001136.8 981458 982774 D Ydr261w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 981458..982774 Saccharomyces cerevisiae 851850 NP_058148.1 CDS YDR261C-D NC_001136.8 987530 992345 R Ydr261c-dp; Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional complement(join(987530..991039,991041..992345)) Saccharomyces cerevisiae 851852 NP_058149.1 CDS YDR261C-C NC_001136.8 991023 992345 R Ydr261c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably non-functional complement(991023..992345) Saccharomyces cerevisiae 851853 NP_010548.1 CDS YDR262W NC_001136.8 993130 993948 D Ydr262wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment 993130..993948 Saccharomyces cerevisiae 851855 NP_010549.1 CDS DIN7 NC_001136.8 994238 995530 R Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; Din7p complement(994238..995530) Saccharomyces cerevisiae 851856 NP_010550.1 CDS AKR1 NC_001136.8 996025 998319 R Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; Akr1p complement(996025..998319) Saccharomyces cerevisiae 851857 NP_010551.1 CDS PEX10 NC_001136.8 998860 999873 D Pex10p; C3HC4-type RING-finger peroxisomal membrane peroxin required for peroxisomal matrix protein import, interacts with Pex12p, links ubiquitin-conjugating Pex4p to import machinery; mutations in human homolog cause various peroxisomal disorders 998860..999873 Saccharomyces cerevisiae 851858 NP_010552.1 CDS YDR266C NC_001136.8 1000100 1002019 R Ydr266cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments;green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; contains a RING finger domain complement(1000100..1002019) Saccharomyces cerevisiae 851859 NP_010553.1 CDS CIA1 NC_001136.8 1002506 1003498 R Essential protein involved in assembly of cytosolic and nuclear iron-sulfur proteins; Cia1p complement(1002506..1003498) Saccharomyces cerevisiae 851860 NP_010554.1 CDS MSW1 NC_001136.8 1004000 1005139 D Msw1p; Mitochondrial tryptophanyl-tRNA synthetase 1004000..1005139 Saccharomyces cerevisiae 851861 NP_010556.1 CDS CCC2 NC_001136.8 1005671 1008685 D Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; Ccc2p 1005671..1008685 Saccharomyces cerevisiae 851862 NP_010558.1 CDS GLO2 NC_001136.8 1009006 1009830 D Glo2p; Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate 1009006..1009830 Saccharomyces cerevisiae 851865 NP_010559.1 CDS DON1 NC_001136.8 1010172 1011269 D Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; Don1p 1010172..1011269 Saccharomyces cerevisiae 851866 NP_010561.1 CDS BSC2 NC_001136.8 1012248 1012955 D Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; Bsc2p 1012248..1012955 Saccharomyces cerevisiae 851868 NP_010562.1 CDS PMP3 NC_001136.8 1013472 1013639 R Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential; Pmp3p complement(1013472..1013639) Saccharomyces cerevisiae 851869 NP_010563.1 CDS MTH1 NC_001136.8 1014397 1015698 R Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; Mth1p complement(1014397..1015698) Saccharomyces cerevisiae 851870 NP_010565.1 CDS RNH202 NC_001136.8 1019364 1020416 D Rnh202p; Ribonuclease H2 subunit, required for RNase H2 activity 1019364..1020416 Saccharomyces cerevisiae 851873 NP_010566.1 CDS RRP45 NC_001136.8 1020743 1021660 D Rrp45p; Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex 1020743..1021660 Saccharomyces cerevisiae 851874 NP_010567.1 CDS PHM6 NC_001136.8 1022003 1022317 R Protein of unknown function, expression is regulated by phosphate levels; Phm6p complement(1022003..1022317) Saccharomyces cerevisiae 851875 NP_010568.1 CDS YDR282C NC_001136.8 1023507 1024751 R Ydr282cp; Putative protein of unknown function complement(1023507..1024751) Saccharomyces cerevisiae 851876 NP_010569.1 CDS GCN2 NC_001136.8 1025066 1030045 R Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; Gcn2p complement(1025066..1030045) Saccharomyces cerevisiae 851877 NP_010570.1 CDS DPP1 NC_001136.8 1030546 1031415 R Dpp1p; Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism complement(1030546..1031415) Saccharomyces cerevisiae 851878 NP_010571.1 CDS ZIP1 NC_001136.8 1032432 1035059 D Zip1p; Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate 1032432..1035059 Saccharomyces cerevisiae 851879 NP_010572.1 CDS YDR286C NC_001136.8 1035227 1035571 R Ydr286cp; Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site complement(1035227..1035571) Saccharomyces cerevisiae 851880 NP_010573.1 CDS INM2 NC_001136.8 1035991 1036869 D Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy; Inm2p 1035991..1036869 Saccharomyces cerevisiae 851881 NP_010574.1 CDS NSE3 NC_001136.8 1037191 1038102 D Nse3p; Essential subunit of the Mms21-Smc5-Smc6 complex; protein of unknown function; required for DNA repair and growth 1037191..1038102 Saccharomyces cerevisiae 851882 NP_010575.1 CDS RTT103 NC_001136.8 1038276 1039505 R Rtt103p; Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition complement(1038276..1039505) Saccharomyces cerevisiae 851884 NP_010577.1 CDS HRQ1 NC_001136.8 1039724 1042957 D Putative DNA helicase; Hrq1p 1039724..1042957 Saccharomyces cerevisiae 851885 NP_010578.1 CDS SRP101 NC_001136.8 1043142 1045007 R Srp101p; Signal recognition particle (SRP) receptor - alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with SRP102p complement(1043142..1045007) Saccharomyces cerevisiae 851886 NP_010579.1 CDS SSD1 NC_001136.8 1045636 1049388 R Ssd1p; Protein with a role in maintenance of cellular integrity, interacts with components of the TOR pathway; ssd1 mutant of a clinical S. cerevisiae strain displays elevated virulence complement(1045636..1049388) Saccharomyces cerevisiae 851887 NP_010580.1 CDS DPL1 NC_001136.8 1050455 1052224 R Dpl1p; Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate complement(1050455..1052224) Saccharomyces cerevisiae 851888 NP_010581.1 CDS HDA2 NC_001136.8 1052619 1054643 R Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; Hda2p complement(1052619..1054643) Saccharomyces cerevisiae 851889 NP_010582.1 CDS MHR1 NC_001136.8 1055208 1055888 D Protein involved in homologous recombination in mitochondria and in transcription regulation in nucleus; binds to activation domains of acidic activators; required for recombination-dependent mtDNA partitioning; Mhr1p 1055208..1055888 Saccharomyces cerevisiae 851890 NP_010583.1 CDS SUR2 NC_001136.8 1056547 1057596 D Sur2p; Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis 1056547..1057596 Saccharomyces cerevisiae 851891 NP_010584.1 CDS ATP5 NC_001136.8 1058172 1058810 R Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated; Atp5p complement(1058172..1058810) Saccharomyces cerevisiae 851892 NP_010585.1 CDS BFR2 NC_001136.8 1059623 1061227 D Essential protein possibly involved in secretion; multicopy suppressor of sensitivity to Brefeldin A; Bfr2p 1059623..1061227 Saccharomyces cerevisiae 851893 NP_010586.1 CDS PRO1 NC_001136.8 1061501 1062787 R Pro1p; Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis complement(1061501..1062787) Saccharomyces cerevisiae 851894 NP_010587.1 CDS CFT1 NC_001136.8 1063348 1067421 D RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF; Cft1p 1063348..1067421 Saccharomyces cerevisiae 851895 NP_010588.1 CDS GPI11 NC_001136.8 1067727 1068386 D Gpi11p; ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog 1067727..1068386 Saccharomyces cerevisiae 851896 NP_010589.1 CDS RSC3 NC_001136.8 1068725 1071382 R Rsc3p; Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p complement(1068725..1071382) Saccharomyces cerevisiae 851897 NP_010590.1 CDS CPR5 NC_001136.8 1071876 1072553 R Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; Cpr5p complement(1071876..1072553) Saccharomyces cerevisiae 851898 NP_010591.1 CDS HNT2 NC_001136.8 1072742 1073484 R Hnt2p; Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins complement(join(1072742..1073308,1073398..1073484)) Saccharomyces cerevisiae 851899 NP_010592.1 CDS YDR306C NC_001136.8 1073731 1075167 R Ydr306cp; F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain complement(1073731..1075167) Saccharomyces cerevisiae 851900 NP_010593.1 CDS YDR307W NC_001136.8 1075861 1077849 D Ydr307wp; Putative protein of unknown function 1075861..1077849 Saccharomyces cerevisiae 851902 NP_010594.1 CDS SRB7 NC_001136.8 1078023 1078445 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p; Srb7p complement(1078023..1078445) Saccharomyces cerevisiae 851903 NP_010595.1 CDS GIC2 NC_001136.8 1079044 1080195 R Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; Gic2p complement(1079044..1080195) Saccharomyces cerevisiae 851904 NP_010596.1 CDS SUM1 NC_001136.8 1081124 1084312 R Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; involved in telomere maintenance, regulated by the pachytene checkpoint; Sum1p complement(1081124..1084312) Saccharomyces cerevisiae 851905 NP_010597.1 CDS TFB1 NC_001136.8 1085062 1086990 D Tfb1p; Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators 1085062..1086990 Saccharomyces cerevisiae 851906 NP_010598.1 CDS SSF2 NC_001136.8 1087578 1088939 D Ssf2p; Protein required for ribosomal large subunit maturation, functionally redundant with Ssf1p; member of the Brix family 1087578..1088939 Saccharomyces cerevisiae 851907 NP_010599.1 CDS PIB1 NC_001136.8 1089216 1090076 R RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain; Pib1p complement(1089216..1090076) Saccharomyces cerevisiae 851908 NP_010600.1 CDS RAD34 NC_001136.8 1090430 1092508 R Rad34p; Protein involved in nucleotide excision repair (NER); homologous to RAD4 complement(1090430..1092508) Saccharomyces cerevisiae 851909 NP_010601.1 CDS IPK1 NC_001136.8 1092738 1093583 R Ipk1p; Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable complement(1092738..1093583) Saccharomyces cerevisiae 851910 NP_010602.1 CDS OMS1 NC_001136.8 1093760 1095175 D Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations; Oms1p 1093760..1095175 Saccharomyces cerevisiae 851911 NP_058150.1 CDS YDR316W-B NC_001136.8 1096061 1101329 D Ydr316w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(1096061..1097365,1097367..1101329) Saccharomyces cerevisiae 851914 NP_058151.1 CDS YDR316W-A NC_001136.8 1096061 1097383 D Ydr316w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1096061..1097383 Saccharomyces cerevisiae 851913 NP_010603.1 CDS HIM1 NC_001136.8 1102181 1103425 D Protein of unknown function involved in DNA repair; Him1p 1102181..1103425 Saccharomyces cerevisiae 851915 NP_010604.2 CDS MCM21 NC_001136.8 1103755 1104944 D Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules; Mcm21p join(1103755..1103806,1103890..1104944) Saccharomyces cerevisiae 851916 NP_010605.1 CDS YFT2 NC_001136.8 1105000 1105824 R Yft2p; Putative protein of unknown function, identified as an ortholog of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens complement(1105000..1105824) Saccharomyces cerevisiae 851917 NP_010606.1 CDS SWA2 NC_001136.8 1106092 1108098 R Swa2p; Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles complement(1106092..1108098) Saccharomyces cerevisiae 851918 NP_710144.1 CDS DAD4 NC_001136.8 1108277 1108495 R Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Dad4p complement(1108277..1108495) Saccharomyces cerevisiae 851919 NP_010607.1 CDS ASP1 NC_001136.8 1108699 1109844 D Cytosolic L-asparaginase, involved in asparagine catabolism; Asp1p 1108699..1109844 Saccharomyces cerevisiae 851920 NP_010608.1 CDS MRPL35 NC_001136.8 1110586 1111689 D Mrpl35p; Mitochondrial ribosomal protein of the large subunit 1110586..1111689 Saccharomyces cerevisiae 851921 NP_010609.1 CDS TIM11 NC_001136.8 1112000 1112290 R Tim11p; Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase complement(1112000..1112290) Saccharomyces cerevisiae 851922 NP_010610.1 CDS PEP7 NC_001136.8 1112477 1114024 R Pep7p; Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance complement(1112477..1114024) Saccharomyces cerevisiae 851923 NP_010611.1 CDS UTP4 NC_001136.8 1114430 1116760 R Utp4p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(1114430..1116760) Saccharomyces cerevisiae 851924 NP_010612.2 CDS YCG1 NC_001136.8 1117121 1120228 D Ycg1p; Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation and for chromatin binding of the condensin complex 1117121..1120228 Saccharomyces cerevisiae 851925 NP_010613.1 CDS YSP2 NC_001136.8 1120604 1124920 R Ysp2p; Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification complement(1120604..1124920) Saccharomyces cerevisiae 851926 NP_010615.1 CDS SKP1 NC_001136.8 1125429 1126013 R Skp1p; Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase complement(1125429..1126013) Saccharomyces cerevisiae 851928 NP_010616.1 CDS PEX3 NC_001136.8 1126265 1127590 R Pex3p; Peroxisomal membrane protein (PMP) required required for the proper localization and stability of PMPs; interacts with Pex19p complement(1126265..1127590) Saccharomyces cerevisiae 851929 NP_010617.1 CDS UBX5 NC_001136.8 1127867 1129369 D Ubx5p; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p 1127867..1129369 Saccharomyces cerevisiae 851930 NP_010618.1 CDS GPI8 NC_001136.8 1129583 1130818 D Gpi8p; ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog 1129583..1130818 Saccharomyces cerevisiae 851931 NP_010619.1 CDS IRC3 NC_001136.8 1130996 1133065 D hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci; Irc3p 1130996..1133065 Saccharomyces cerevisiae 851932 NP_010620.1 CDS YDR333C NC_001136.8 1133255 1135426 R Ydr333cp; Putative protein of unknown function complement(1133255..1135426) Saccharomyces cerevisiae 851933 NP_010621.1 CDS SWR1 NC_001136.8 1135927 1140471 D Swr1p; Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A 1135927..1140471 Saccharomyces cerevisiae 851934 NP_010622.1 CDS MSN5 NC_001136.8 1141162 1144836 D Msn5p; Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for export of several proteins including Swi6p, Far1p, and Pho4p; cargo dissociation involves binding to RanGTP 1141162..1144836 Saccharomyces cerevisiae 851935 NP_010623.1 CDS YDR336W NC_001136.8 1145086 1146030 D Ydr336wp; Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene 1145086..1146030 Saccharomyces cerevisiae 851936 NP_010624.1 CDS MRPS28 NC_001136.8 1146313 1147173 D Mrps28p; Mitochondrial ribosomal protein of the small subunit 1146313..1147173 Saccharomyces cerevisiae 851937 NP_010625.1 CDS YDR338C NC_001136.8 1147373 1149460 R Ydr338cp complement(1147373..1149460) Saccharomyces cerevisiae 851938 NP_010626.1 CDS FCF1 NC_001136.8 1149945 1150514 R Fcf1p; Essential nucleolar protein that is a component of the SSU (small subunit) processome involved in the pre-rRNA processing steps of 40S ribosomal subunit biogenesis; contains a PINc domain; copurifies with Faf1p complement(1149945..1150514) Saccharomyces cerevisiae 851939 NP_010628.1 CDS YDR341C NC_001136.8 1151797 1153620 R Ydr341cp; Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(1151797..1153620) Saccharomyces cerevisiae 851942 NP_010629.1 CDS HXT7 NC_001136.8 1154210 1155922 R Hxt7p; High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels complement(1154210..1155922) Saccharomyces cerevisiae 851943 NP_010630.1 CDS HXT6 NC_001136.8 1159602 1161314 R Hxt6p; High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3 complement(1159602..1161314) Saccharomyces cerevisiae 851944 NP_010632.1 CDS HXT3 NC_001136.8 1162951 1164654 R Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions; Hxt3p complement(1162951..1164654) Saccharomyces cerevisiae 851946 NP_010633.1 CDS SVF1 NC_001136.8 1167208 1168653 R Svf1p; Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis complement(1167208..1168653) Saccharomyces cerevisiae 851947 NP_010634.1 CDS MRP1 NC_001136.8 1169172 1170137 D Mrp1p; Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein 1169172..1170137 Saccharomyces cerevisiae 851948 NP_010635.1 CDS YDR348C NC_001136.8 1170320 1171819 R Ydr348cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud neck; potential Cdc28p substrate complement(1170320..1171819) Saccharomyces cerevisiae 851949 NP_010636.1 CDS YPS7 NC_001136.8 1172380 1174170 R Yps7p; Putative GPI-anchored aspartic protease, located in the cytoplasm and endoplasmic reticulum complement(1172380..1174170) Saccharomyces cerevisiae 851950 NP_010637.1 CDS ATP22 NC_001136.8 1176332 1178167 R Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp22p complement(1176332..1178167) Saccharomyces cerevisiae 851952 NP_010638.1 CDS SBE2 NC_001136.8 1178659 1181253 D Sbe2p; Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth 1178659..1181253 Saccharomyces cerevisiae 851953 NP_010639.1 CDS YDR352W NC_001136.8 1181794 1182747 D Ydr352wp; Putative protein of unknown function 1181794..1182747 Saccharomyces cerevisiae 851954 NP_010640.1 CDS TRR1 NC_001136.8 1183292 1184251 D Trr1p; Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress 1183292..1184251 Saccharomyces cerevisiae 851955 NP_010641.1 CDS TRP4 NC_001136.8 1184740 1185882 D Trp4p; Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis 1184740..1185882 Saccharomyces cerevisiae 851956 NP_010643.1 CDS SPC110 NC_001136.8 1186100 1188934 D Spc110p; Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner 1186100..1188934 Saccharomyces cerevisiae 851957 NP_010644.1 CDS YDR357C NC_001136.8 1189192 1189560 R Ydr357cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(1189192..1189560) Saccharomyces cerevisiae 851959 NP_010645.1 CDS GGA1 NC_001136.8 1190052 1191725 D Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; Gga1p 1190052..1191725 Saccharomyces cerevisiae 851960 NP_010646.2 CDS EAF1 NC_001136.8 1191929 1194877 R Eaf1p; Component of the NuA4 histone acetyltransferase complex complement(1191929..1194877) Saccharomyces cerevisiae 851962 NP_010648.1 CDS BCP1 NC_001136.8 1195404 1196255 R Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport; Bcp1p complement(1195404..1196255) Saccharomyces cerevisiae 851963 NP_010649.1 CDS TFC6 NC_001136.8 1196671 1198689 R Tfc6p; One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 complement(1196671..1198689) Saccharomyces cerevisiae 851964 NP_010650.1 CDS ESC2 NC_001136.8 1199175 1200545 D Protein involved in mating-type locus silencing, interacts with Sir2p; probably functions to recruit or stabilize Sir proteins; Esc2p 1199175..1200545 Saccharomyces cerevisiae 851965 NP_010651.1 CDS SEM1 NC_001136.8 1202120 1202389 D Sem1p; Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; ortholog of human DSS1 1202120..1202389 Saccharomyces cerevisiae 851967 NP_010652.1 CDS CDC40 NC_001136.8 1202835 1204202 R Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats; Cdc40p complement(1202835..1204202) Saccharomyces cerevisiae 851968 NP_010653.1 CDS ESF1 NC_001136.8 1204489 1206375 R Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels; Esf1p complement(1204489..1206375) Saccharomyces cerevisiae 851969 NP_058152.1 CDS YDR365W-B NC_001136.8 1206989 1212257 D Ydr365w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(1206989..1208293,1208295..1212257) Saccharomyces cerevisiae 851971 NP_058153.1 CDS YDR365W-A NC_001136.8 1206989 1208311 D Ydr365w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 1206989..1208311 Saccharomyces cerevisiae 851970 NP_010654.1 CDS YDR366C NC_001136.8 1212430 1212828 R Ydr366cp complement(1212430..1212828) Saccharomyces cerevisiae 851972 NP_010655.1 CDS YDR367W NC_001136.8 1212840 1213606 D Ydr367wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern join(1212840..1212869,1212971..1213606) Saccharomyces cerevisiae 851973 NP_010656.1 CDS YPR1 NC_001136.8 1213896 1214834 D Ypr1p; 2-methylbutyraldehyde reductase, may be involved in isoleucine catabolism 1213896..1214834 Saccharomyces cerevisiae 851974 NP_010657.1 CDS XRS2 NC_001136.8 1215008 1217572 R Xrs2p; Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling complement(1215008..1217572) Saccharomyces cerevisiae 851975 NP_010658.1 CDS YDR370C NC_001136.8 1217774 1219102 R Ydr370cp; Putative protein of unknown function complement(1217774..1219102) Saccharomyces cerevisiae 851976 NP_010659.1 CDS CTS2 NC_001136.8 1219405 1220940 D Sporulation-specific chitinase; Cts2p 1219405..1220940 Saccharomyces cerevisiae 851977 NP_010660.1 CDS VPS74 NC_001136.8 1221104 1222141 R Vps74p; Non-essential protein of unknown function involved in vacuolar protein sorting; belongs to a family of cytosolic Golgi-associated proteins suggesting that it may play a role in secretion; also detected in the nucleus complement(1221104..1222141) Saccharomyces cerevisiae 851978 NP_010661.1 CDS FRQ1 NC_001136.8 1222751 1223323 D N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; Frq1p 1222751..1223323 Saccharomyces cerevisiae 851979 NP_010662.1 CDS YDR374C NC_001136.8 1223466 1224386 R Ydr374cp; Putative protein of unknown function complement(1223466..1224386) Saccharomyces cerevisiae 851980 NP_001032576.1 CDS YDR374W-A NC_001136.8 1224749 1225018 D Ydr374w-ap; Putative protein of unknown function 1224749..1225018 Saccharomyces cerevisiae 3799971 NP_010663.1 CDS BCS1 NC_001136.8 1225158 1226528 R Protein of the mitochondrial inner membrane that functions as an ATP-dependent chaperone, required for the incorporation of the Rip1p and Qcr10p subunits into the cytochrome bc(1) complex; member of the CDC48/PAS1/SEC18 ATPase family; Bcs1p complement(1225158..1226528) Saccharomyces cerevisiae 851981 NP_010664.1 CDS ARH1 NC_001136.8 1226814 1228295 D Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability; Arh1p 1226814..1228295 Saccharomyces cerevisiae 851982 NP_010665.1 CDS ATP17 NC_001136.8 1228603 1228908 D Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp17p 1228603..1228908 Saccharomyces cerevisiae 851983 NP_010666.2 CDS LSM6 NC_001136.8 1229341 1229601 R Lsm6p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA complement(1229341..1229601) Saccharomyces cerevisiae 851984 NP_010667.1 CDS RGA2 NC_001136.8 1230159 1233188 D Rga2p; GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth 1230159..1233188 Saccharomyces cerevisiae 851985 NP_076888.1 CDS YDR379C-A NC_001136.8 1233270 1233509 R hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36.; Ydr379c-ap complement(1233270..1233509) Saccharomyces cerevisiae 851986 NP_010668.1 CDS ARO10 NC_001136.8 1234210 1236117 D Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; Aro10p 1234210..1236117 Saccharomyces cerevisiae 851987 NP_010669.1 CDS YRA1 NC_001136.8 1236550 1237996 D Yra1p; Nuclear protein that binds to RNA and to Mex67p, required for export of poly(A)+ mRNA from the nucleus; member of the REF (RNA and export factor binding proteins) family; another family member, Yra2p, can substitute for Yra1p function join(1236550..1236834,1237601..1237996) Saccharomyces cerevisiae 851988 NP_219495.2 CDS YDR381C-A NC_001136.8 1238304 1238842 R Ydr381c-ap; Protein of unknown function, localized to the mitochondrial outer membrane complement(join(1238304..1238622,1238817..1238842)) Saccharomyces cerevisiae 851989 NP_010670.1 CDS RPP2B NC_001136.8 1239484 1239816 D Rpp2bp; Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm 1239484..1239816 Saccharomyces cerevisiae 851990 NP_010671.2 CDS NKP1 NC_001136.8 1239952 1240668 R Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p- Nkp1p-Nkp2p-Ame1p-Mtw1p); Nkp1p complement(1239952..1240668) Saccharomyces cerevisiae 851991 NP_010672.1 CDS ATO3 NC_001136.8 1241196 1242023 R Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters; Ato3p complement(1241196..1242023) Saccharomyces cerevisiae 851992 NP_010673.1 CDS EFT2 NC_001136.8 1243222 1245750 D Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; Eft2p 1243222..1245750 Saccharomyces cerevisiae 851993 NP_010674.1 CDS MUS81 NC_001136.8 1246076 1247974 D Mus81p; Helix-hairpin-helix protein, involved in DNA repair and replication fork stability; functions as an endonuclease in complex with Mms4p; interacts with Rad54p 1246076..1247974 Saccharomyces cerevisiae 851994 NP_010675.1 CDS YDR387C NC_001136.8 1248146 1249813 R Ydr387cp; Putative transporter, member of the sugar porter family; YDR387C is not an essential gene complement(1248146..1249813) Saccharomyces cerevisiae 851995 NP_010676.1 CDS RVS167 NC_001136.8 1250178 1251626 D Rvs167p; Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin 1250178..1251626 Saccharomyces cerevisiae 851996 NP_010677.1 CDS SAC7 NC_001136.8 1252529 1254493 D Sac7p; GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate 1252529..1254493 Saccharomyces cerevisiae 851997 NP_010678.1 CDS UBA2 NC_001136.8 1254929 1256839 R Uba2p; Nuclear protein that acts as a heterodimer with Aos1p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability complement(1254929..1256839) Saccharomyces cerevisiae 851998 NP_010679.1 CDS YDR391C NC_001136.8 1257350 1258048 R Ydr391cp; Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus complement(1257350..1258048) Saccharomyces cerevisiae 852000 NP_010680.1 CDS SPT3 NC_001136.8 1258688 1259701 D Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; Spt3p 1258688..1259701 Saccharomyces cerevisiae 852001 NP_010681.1 CDS SHE9 NC_001136.8 1259893 1261263 D She9p; Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex 1259893..1261263 Saccharomyces cerevisiae 852002 NP_010682.1 CDS RPT3 NC_001136.8 1261673 1262959 D Rpt3p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B 1261673..1262959 Saccharomyces cerevisiae 852003 NP_010683.1 CDS SXM1 NC_001136.8 1263316 1266150 D Sxm1p; Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 1263316..1266150 Saccharomyces cerevisiae 852004 NP_010685.1 CDS NCB2 NC_001136.8 1266358 1266890 R Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; Ncb2p complement(join(1266358..1266761,1266854..1266890)) Saccharomyces cerevisiae 852006 NP_010686.1 CDS UTP5 NC_001136.8 1267463 1269394 D Utp5p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA 1267463..1269394 Saccharomyces cerevisiae 852007 NP_010687.1 CDS HPT1 NC_001136.8 1270060 1270725 D Hpt1p; Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and guanosine monophosphate; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome 1270060..1270725 Saccharomyces cerevisiae 852008 NP_010688.2 CDS URH1 NC_001136.8 1271055 1272077 D Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways; Urh1p 1271055..1272077 Saccharomyces cerevisiae 852009 NP_010690.1 CDS DIT2 NC_001136.8 1272226 1273695 R N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s; Dit2p complement(1272226..1273695) Saccharomyces cerevisiae 852011 NP_010691.1 CDS DIT1 NC_001136.8 1274594 1276204 D Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure; Dit1p 1274594..1276204 Saccharomyces cerevisiae 852012 NP_010692.1 CDS RPB7 NC_001136.8 1276646 1277161 R Rpb7p; RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p complement(1276646..1277161) Saccharomyces cerevisiae 852013 NP_010693.1 CDS MRP20 NC_001136.8 1277638 1278429 D Mrp20p; Mitochondrial ribosomal protein of the large subunit 1277638..1278429 Saccharomyces cerevisiae 852014 NP_010694.1 CDS PDR15 NC_001136.8 1279202 1283791 D Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; Pdr15p 1279202..1283791 Saccharomyces cerevisiae 852015 NP_010695.1 CDS TRS120 NC_001136.8 1284061 1287930 R Trs120p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic complement(1284061..1287930) Saccharomyces cerevisiae 852016 NP_010696.1 CDS ADE8 NC_001136.8 1288207 1288851 R Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; Ade8p complement(1288207..1288851) Saccharomyces cerevisiae 852017 NP_010697.1 CDS SIZ1 NC_001136.8 1289398 1292112 D Siz1p; SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring 1289398..1292112 Saccharomyces cerevisiae 852018 NP_010698.1 CDS STE14 NC_001136.8 1292364 1293083 R Ste14p; Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane complement(1292364..1293083) Saccharomyces cerevisiae 852019 NP_010699.1 CDS DFM1 NC_001136.8 1293361 1294386 R ER localized derlin-like family member involved in ER stress and homeostasis; not involved in ERAD or substrate retrotranslocation; interacts with CDC48; contains four transmembrane domains and two SHP boxes; Dfm1p complement(1293361..1294386) Saccharomyces cerevisiae 852020 NP_010700.1 CDS RRP17 NC_001136.8 1294685 1295392 D Rrp17p; Protein required for cell viability 1294685..1295392 Saccharomyces cerevisiae 852021 NP_010702.1 CDS ERD1 NC_001136.8 1295590 1296678 R Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p); Erd1p complement(1295590..1296678) Saccharomyces cerevisiae 852023 NP_010703.1 CDS YDR415C NC_001136.8 1297029 1298153 R Ydr415cp; Putative protein of unknown function complement(1297029..1298153) Saccharomyces cerevisiae 852024 NP_010704.1 CDS SYF1 NC_001136.8 1298424 1301003 D Syf1p; Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression; similar to Drosophila crooked neck protein 1298424..1301003 Saccharomyces cerevisiae 852025 NP_010706.1 CDS RPL12B NC_001136.8 1301608 1302105 D Rpl12bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins 1301608..1302105 Saccharomyces cerevisiae 852026 NP_010707.1 CDS RAD30 NC_001136.8 1303166 1305064 D Rad30p; DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteins 1303166..1305064 Saccharomyces cerevisiae 852028 NP_010708.1 CDS HKR1 NC_001136.8 1306259 1311667 D Serine/threonine rich cell surface protein; osmosensor; involved in the regulation of cell wall beta-1,3 glucan synthesis, bud site selection and hyperosmotic response; overexpression confers resistance to Hansenula mrakii killer toxin HM-1; Hkr1p 1306259..1311667 Saccharomyces cerevisiae 852030 NP_010709.1 CDS ARO80 NC_001136.8 1312032 1314884 D Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids; Aro80p 1312032..1314884 Saccharomyces cerevisiae 852031 NP_010710.2 CDS SIP1 NC_001136.8 1315318 1317765 R Sip1p; Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions complement(1315318..1317765) Saccharomyces cerevisiae 852032 NP_010711.1 CDS CAD1 NC_001136.8 1318038 1319267 R Cad1p; AP-1-like bZIP transcriptional activator involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; 5' UTR contains uORFs complement(1318038..1319267) Saccharomyces cerevisiae 852033 NP_010712.1 CDS DYN2 NC_001136.8 1319379 1319833 R Dyn2p; Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex complement(join(1319379..1319609,1319690..1319712,1319809..1319833)) Saccharomyces cerevisiae 852034 NP_010713.1 CDS SNX41 NC_001136.8 1320056 1321933 D Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; forms a complex with Snx4p and Atg20p; Snx41p 1320056..1321933 Saccharomyces cerevisiae 852035 NP_010715.1 CDS RPN9 NC_001136.8 1322197 1323378 D Rpn9p; Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects 1322197..1323378 Saccharomyces cerevisiae 852037 NP_010716.1 CDS YDR428C NC_001136.8 1323446 1324231 R Ydr428cp; Putative alpha/beta-serine hydrolase belonging to the subfamily of hormone-sensitive lipase-like hydrolases complement(1323446..1324231) Saccharomyces cerevisiae 852038 NP_010717.1 CDS TIF35 NC_001136.8 1324469 1325293 R Tif35p; Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation complement(1324469..1325293) Saccharomyces cerevisiae 852039 NP_010718.1 CDS CYM1 NC_001136.8 1325493 1328462 R Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology; Cym1p complement(1325493..1328462) Saccharomyces cerevisiae 852041 NP_010720.1 CDS NPL3 NC_001136.8 1328775 1330019 D RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; dissociation from mRNAs is promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; Npl3p 1328775..1330019 Saccharomyces cerevisiae 852042 NP_010722.1 CDS GPI17 NC_001136.8 1331229 1332833 D Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Sp homolog; Gpi17p 1331229..1332833 Saccharomyces cerevisiae 852044 NP_010723.1 CDS PPM1 NC_001136.8 1332975 1333961 R Ppm1p; Carboxyl methyl transferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits complement(1332975..1333961) Saccharomyces cerevisiae 852045 NP_010724.1 CDS PPZ2 NC_001136.8 1334813 1336945 D Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance; Ppz2p 1334813..1336945 Saccharomyces cerevisiae 852046 NP_010725.1 CDS GPI19 NC_001136.8 1337344 1337766 D Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P; Gpi19p 1337344..1337766 Saccharomyces cerevisiae 852047 NP_010726.1 CDS THI74 NC_001136.8 1338266 1339378 D Thi74p; Mitochondrial transporter repressible by thiamine 1338266..1339378 Saccharomyces cerevisiae 852048 NP_010727.1 CDS LRS4 NC_001136.8 1339668 1340711 D Protein involved in rDNA silencing; positively charged coiled-coil protein with limited similarity to myosin; Lrs4p 1339668..1340711 Saccharomyces cerevisiae 852049 NP_010728.1 CDS DOT1 NC_001136.8 1342485 1344233 D Nucleosomal histone H3-Lys79 methylase, associates with transcriptionally active genes, functions in gene silencing at telomeres, most likely by directly modulating chromatin structure and Sir protein localization; Dot1p 1342485..1344233 Saccharomyces cerevisiae 852050 NP_010729.1 CDS APT2 NC_001136.8 1344509 1345054 R Apt2p; Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity complement(1344509..1345054) Saccharomyces cerevisiae 852051 NP_010731.1 CDS SSN2 NC_001136.8 1345668 1349930 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation; Ssn2p complement(1345668..1349930) Saccharomyces cerevisiae 852053 NP_010732.1 CDS YDR444W NC_001136.8 1350282 1352345 D Ydr444wp; Putative protein of unknown function 1350282..1352345 Saccharomyces cerevisiae 852054 NP_010734.1 CDS ECM11 NC_001136.8 1353717 1354625 D Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure; Ecm11p 1353717..1354625 Saccharomyces cerevisiae 852057 NP_010735.1 CDS RPS17B NC_001136.8 1354821 1355545 R Rps17bp; Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similarity to rat S17 ribosomal protein complement(join(1354821..1355228,1355543..1355545)) Saccharomyces cerevisiae 852058 NP_010736.1 CDS ADA2 NC_001136.8 1356057 1357361 D Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes; Ada2p 1356057..1357361 Saccharomyces cerevisiae 852059 NP_010737.1 CDS UTP6 NC_001136.8 1357572 1358894 R Utp6p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(1357572..1358894) Saccharomyces cerevisiae 852060 NP_010738.1 CDS RPS18A NC_001136.8 1359915 1360790 D Rps18ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins join(1359915..1359961,1360397..1360790) Saccharomyces cerevisiae 852061 NP_010739.1 CDS YHP1 NC_001136.8 1361112 1362173 R Yhp1p; One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval complement(1361112..1362173) Saccharomyces cerevisiae 852062 NP_010740.1 CDS PPN1 NC_001136.8 1362870 1364894 D Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; Ppn1p 1362870..1364894 Saccharomyces cerevisiae 852063 NP_010741.1 CDS TSA2 NC_001136.8 1365064 1365654 R Tsa2p; Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants complement(1365064..1365654) Saccharomyces cerevisiae 852064 NP_010742.1 CDS GUK1 NC_001136.8 1366256 1366819 R Guk1p; Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins complement(1366256..1366819) Saccharomyces cerevisiae 852065 NP_010744.1 CDS NHX1 NC_001136.8 1367477 1369378 D Nhx1p; Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock 1367477..1369378 Saccharomyces cerevisiae 852066 NP_010745.1 CDS TOM1 NC_001136.8 1369782 1379588 D Tom1p; E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators 1369782..1379588 Saccharomyces cerevisiae 852068 NP_010746.1 CDS HEH2 NC_001136.8 1380047 1382038 R Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; Heh2p complement(1380047..1382038) Saccharomyces cerevisiae 852069 NP_010747.1 CDS PFA5 NC_001136.8 1382311 1383435 R Pfa5p; Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain complement(1382311..1383435) Saccharomyces cerevisiae 852070 NP_010748.1 CDS TFB3 NC_001136.8 1383803 1384768 D Tfb3p; Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit 1383803..1384768 Saccharomyces cerevisiae 852071 NP_010749.1 CDS MFA1 NC_001136.8 1385168 1385278 D Mfa1p; Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 1385168..1385278 Saccharomyces cerevisiae 852072 NP_001032577.1 CDS YDR461C-A NC_001136.8 1385516 1385758 R Ydr461c-ap; Putative protein of unknown function complement(1385516..1385758) Saccharomyces cerevisiae 3799972 NP_010750.1 CDS MRPL28 NC_001136.8 1386065 1386508 D Mrpl28p; Mitochondrial ribosomal protein of the large subunit 1386065..1386508 Saccharomyces cerevisiae 852073 NP_010751.2 CDS STP1 NC_001136.8 1386808 1388367 D Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; Stp1p 1386808..1388367 Saccharomyces cerevisiae 852074 NP_010752.1 CDS SPP41 NC_001136.8 1388864 1393171 D Spp41p; Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 1388864..1393171 Saccharomyces cerevisiae 852075 NP_010753.1 CDS RMT2 NC_001136.8 1393328 1394566 R Rmt2p; Arginine methyltransferase; ribosomal protein L12 is a substrate complement(1393328..1394566) Saccharomyces cerevisiae 852076 NP_010754.1 CDS PKH3 NC_001136.8 1395113 1397809 D Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant; Pkh3p 1395113..1397809 Saccharomyces cerevisiae 852077 NP_010756.1 CDS TLG1 NC_001136.8 1398018 1398692 R Tlg1p; Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p complement(1398018..1398692) Saccharomyces cerevisiae 852079 NP_010757.1 CDS SDC1 NC_001136.8 1399007 1399534 D Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; has similarity to C. elegans Dpy-30; Sdc1p 1399007..1399534 Saccharomyces cerevisiae 852080 NP_010758.2 CDS UGO1 NC_001136.8 1399698 1401206 R Ugo1p; Protein of unknown function; outer membrane component of the mitochondrial fusion machinery; Ugo1p bind directly to Fzo1p and Mgm1p and thereby link these two GTPases during mitochondrial fusion complement(1399698..1401206) Saccharomyces cerevisiae 852081 NP_010759.1 CDS RPL27B NC_001136.8 1401762 1402556 D Rpl27bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Ap and has similarity to rat L27 ribosomal protein join(1401762..1401792,1402177..1402556) Saccharomyces cerevisiae 852082 NP_010760.1 CDS TRS31 NC_001136.8 1403314 1404165 D Trs31p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic 1403314..1404165 Saccharomyces cerevisiae 852083 NP_010761.1 CDS PRP3 NC_001136.8 1404437 1405846 R Splicing factor, component of the U4/U6-U5 snRNP complex; Prp3p complement(1404437..1405846) Saccharomyces cerevisiae 852084 NP_010763.2 CDS JIP4 NC_001136.8 1407456 1410086 R Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; Jip4p complement(1407456..1410086) Saccharomyces cerevisiae 852086 NP_010764.1 CDS YDR476C NC_001136.8 1410445 1411119 R Ydr476cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene complement(1410445..1411119) Saccharomyces cerevisiae 852087 NP_010765.1 CDS SNF1 NC_001136.8 1412365 1414266 D Snf1p; AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis 1412365..1414266 Saccharomyces cerevisiae 852088 NP_010766.1 CDS SNM1 NC_001136.8 1414567 1415163 D Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP; Snm1p 1414567..1415163 Saccharomyces cerevisiae 852089 NP_010767.1 CDS PEX29 NC_001136.8 1415202 1416866 R Pex29p; Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p complement(1415202..1416866) Saccharomyces cerevisiae 852090 NP_010768.1 CDS DIG2 NC_001136.8 1417391 1418362 D Regulatory protein of unknown function, pheromone-inducible, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription; Dig2p 1417391..1418362 Saccharomyces cerevisiae 852091 NP_010769.1 CDS PHO8 NC_001136.8 1418542 1420242 R Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of inorganic phosphate and by a system consisting of Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; Pho8p complement(1418542..1420242) Saccharomyces cerevisiae 852092 NP_010770.1 CDS CWC21 NC_001136.8 1420423 1420830 R Cwc21p; Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; may bind RNA; has similarity to S. pombe Cwf21p complement(1420423..1420830) Saccharomyces cerevisiae 852093 NP_010771.1 CDS KRE2 NC_001136.8 1421149 1422477 D Kre2p; Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation 1421149..1422477 Saccharomyces cerevisiae 852094 NP_010772.1 CDS VPS52 NC_001136.8 1422755 1424680 D Vps52p; Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin 1422755..1424680 Saccharomyces cerevisiae 852095 NP_010773.2 CDS VPS72 NC_001136.8 1424812 1427199 R Vps72p; Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting complement(1424812..1427199) Saccharomyces cerevisiae 852096 NP_010774.2 CDS VPS60 NC_001136.8 1427423 1428112 R Vps60p; Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting specific cargo proteins for degradation complement(1427423..1428112) Saccharomyces cerevisiae 852097 NP_010775.1 CDS RIB3 NC_001136.8 1428346 1428972 R Rib3p; 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration complement(1428346..1428972) Saccharomyces cerevisiae 852098 NP_010776.1 CDS PAC11 NC_001136.8 1429180 1430781 R Pac11p; Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 complement(1429180..1430781) Saccharomyces cerevisiae 852099 NP_010777.1 CDS SLD5 NC_001136.8 1431004 1431888 D Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery; Sld5p 1431004..1431888 Saccharomyces cerevisiae 852100 NP_010778.1 CDS PKH1 NC_001136.8 1431960 1434260 R Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; Pkh1p complement(1431960..1434260) Saccharomyces cerevisiae 852101 NP_010780.1 CDS IZH1 NC_001136.8 1434916 1435866 D Membrane protein involved in zinc metabolism, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; Izh1p 1434916..1435866 Saccharomyces cerevisiae 852102 NP_010781.1 CDS AIM8 NC_001136.8 1436209 1436580 D Protein of unknown function; non-tagged protein detected in purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss and severe growth defect in minimal glycerol media; Aim8p 1436209..1436580 Saccharomyces cerevisiae 852104 NP_010782.2 CDS RSM28 NC_001136.8 1436922 1438007 D Rsm28p; Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation 1436922..1438007 Saccharomyces cerevisiae 852105 NP_010783.1 CDS VPS3 NC_001136.8 1438107 1441142 R Vps3p; Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase complement(1438107..1441142) Saccharomyces cerevisiae 852106 NP_010784.1 CDS PUF6 NC_001136.8 1441425 1443395 R Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; Puf6p complement(1441425..1443395) Saccharomyces cerevisiae 852107 NP_010785.1 CDS ITR1 NC_001136.8 1443705 1445459 R Myo-inositol transporter with strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; Itr1p complement(1443705..1445459) Saccharomyces cerevisiae 852108 NP_010786.1 CDS SEC20 NC_001136.8 1445835 1446986 R Sec20p; Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; forms a complex with the cytosolic Tip20p complement(1445835..1446986) Saccharomyces cerevisiae 852109 NP_010787.1 CDS LCD1 NC_001136.8 1447822 1450065 D Lcd1p; Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP 1447822..1450065 Saccharomyces cerevisiae 852110 NP_010788.1 CDS RPL37B NC_001136.8 1450190 1450845 R Rpl37bp; Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Ap and to rat L37 ribosomal protein complement(join(1450190..1450449,1450839..1450845)) Saccharomyces cerevisiae 852111 NP_010789.1 CDS PLM2 NC_001136.8 1451345 1452910 D Protein required for partitioning of the 2-micron plasmid; Plm2p 1451345..1452910 Saccharomyces cerevisiae 852112 NP_010790.1 CDS SAM2 NC_001136.8 1453302 1454456 R Sam2p; S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) complement(1453302..1454456) Saccharomyces cerevisiae 852113 NP_010791.1 CDS LPP1 NC_001136.8 1455034 1455858 R Lpp1p; Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA complement(1455034..1455858) Saccharomyces cerevisiae 852114 NP_010792.1 CDS SPG3 NC_001136.8 1456303 1456686 R Spg3p; Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources complement(1456303..1456686) Saccharomyces cerevisiae 852115 NP_010793.1 CDS PSP1 NC_001136.8 1456687 1459212 R Psp1p; Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition complement(1456687..1459212) Saccharomyces cerevisiae 852116 NP_010794.1 CDS YDR506C NC_001136.8 1459720 1461546 R Ydr506cp; Possible membrane-localized protein complement(1459720..1461546) Saccharomyces cerevisiae 852117 NP_010795.1 CDS GIN4 NC_001136.8 1462350 1465778 R Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p; Gin4p complement(1462350..1465778) Saccharomyces cerevisiae 852119 NP_010796.1 CDS GNP1 NC_001136.8 1466445 1468436 R High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; Gnp1p complement(1466445..1468436) Saccharomyces cerevisiae 852121 NP_010798.1 CDS SMT3 NC_001136.8 1469392 1469697 D Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; Smt3p 1469392..1469697 Saccharomyces cerevisiae 852122 NP_010799.1 CDS ACN9 NC_001136.8 1470009 1470410 D Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes; Acn9p 1470009..1470410 Saccharomyces cerevisiae 852123 NP_010800.1 CDS EMI1 NC_001136.8 1470492 1471055 R Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; Emi1p complement(1470492..1471055) Saccharomyces cerevisiae 852125 NP_010801.1 CDS GRX2 NC_001136.8 1471009 1471440 D Grx2p; Cytoplasmic glutaredoxin, thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress 1471009..1471440 Saccharomyces cerevisiae 852124 NP_010802.1 CDS YDR514C NC_001136.8 1471540 1472991 R Ydr514cp; Putative protein of unknown function complement(1471540..1472991) Saccharomyces cerevisiae 852126 NP_010803.1 CDS SLF1 NC_001136.8 1473421 1474764 D Slf1p; RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts 1473421..1474764 Saccharomyces cerevisiae 852127 NP_010804.1 CDS EMI2 NC_001136.8 1474966 1476468 R Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression is regulated by glucose-repression transcription factors Mig1/2p; Emi2p complement(1474966..1476468) Saccharomyces cerevisiae 852128 NP_010805.1 CDS GRH1 NC_001136.8 1477231 1478349 D Grh1p; Acetylated, cis-golgi localized protein involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes 1477231..1478349 Saccharomyces cerevisiae 852129 NP_010806.1 CDS EUG1 NC_001136.8 1478600 1480153 D Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER; Eug1p 1478600..1480153 Saccharomyces cerevisiae 852130 NP_010807.1 CDS FPR2 NC_001136.8 1480417 1480824 D Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; Fpr2p 1480417..1480824 Saccharomyces cerevisiae 852131 NP_010808.1 CDS YDR520C NC_001136.8 1481077 1483395 R Ydr520cp; Putative transcription factor; contains the (Zn(II)2Cys6 motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is viable and sensitive to caffeine complement(1481077..1483395) Saccharomyces cerevisiae 852133 NP_010810.1 CDS SPS2 NC_001136.8 1483787 1485295 R Sps2p; Protein expressed during sporulation, redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component complement(1483787..1485295) Saccharomyces cerevisiae 852134 NP_010811.1 CDS SPS1 NC_001136.8 1485558 1487030 R Sps1p; Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis complement(1485558..1487030) Saccharomyces cerevisiae 852135 NP_010812.1 CDS AGE1 NC_001136.8 1487534 1488982 R Age1p; ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif complement(1487534..1488982) Saccharomyces cerevisiae 852136 NP_878066.1 CDS YDR524W-C NC_001136.8 1489395 1489484 D Ydr524w-cp; Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin 1489395..1489484 Saccharomyces cerevisiae 1466437 NP_878067.1 CDS YDR524C-B NC_001136.8 1489590 1489790 R Ydr524c-bp; Putative protein of unknown function complement(1489590..1489790) Saccharomyces cerevisiae 1466438 NP_010814.1 CDS SNA2 NC_001136.8 1490588 1490827 D Sna2p; Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 1490588..1490827 Saccharomyces cerevisiae 852138 NP_010816.1 CDS RBA50 NC_001136.8 1491086 1492405 D Rba50p; Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 1491086..1492405 Saccharomyces cerevisiae 852139 NP_010817.1 CDS HLR1 NC_001136.8 1494578 1495849 D Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; Hlr1p 1494578..1495849 Saccharomyces cerevisiae 852141 NP_010818.1 CDS QCR7 NC_001136.8 1496157 1496540 R Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly; Qcr7p complement(1496157..1496540) Saccharomyces cerevisiae 852142 NP_010819.1 CDS APA2 NC_001136.8 1496783 1497760 R Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p; Apa2p complement(1496783..1497760) Saccharomyces cerevisiae 852143 NP_010820.1 CDS YDR531W NC_001136.8 1498224 1499327 D Ydr531wp; Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway leading to coenzyme A1 1498224..1499327 Saccharomyces cerevisiae 852144 NP_010821.1 CDS YDR532C NC_001136.8 1499388 1500545 R Ydr532cp; Protein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p complement(1499388..1500545) Saccharomyces cerevisiae 852145 NP_010822.1 CDS HSP31 NC_001136.8 1501439 1502152 R Possible chaperone and cysteine protease with similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; Hsp31p complement(1501439..1502152) Saccharomyces cerevisiae 852146 NP_010823.1 CDS FIT1 NC_001136.8 1503305 1504891 R Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall; Fit1p complement(1503305..1504891) Saccharomyces cerevisiae 852147 NP_010825.1 CDS STL1 NC_001136.8 1507996 1509705 D Stl1p; Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock 1507996..1509705 Saccharomyces cerevisiae 852149 NP_010827.1 CDS PAD1 NC_001136.8 1510892 1511620 D Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiX; Pad1p 1510892..1511620 Saccharomyces cerevisiae 852150 NP_010828.1 CDS YDR539W NC_001136.8 1512084 1513595 D Ydr539wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR539W is not an essential gene; homolog of E. coli UbiD 1512084..1513595 Saccharomyces cerevisiae 852152 NP_010829.1 CDS IRC4 NC_001136.8 1517120 1517659 R hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci; Irc4p complement(1517120..1517659) Saccharomyces cerevisiae 852153 NP_010830.1 CDS YDR541C NC_001136.8 1519648 1520682 R Ydr541cp; Putative dihydrokaempferol 4-reductase complement(1519648..1520682) Saccharomyces cerevisiae 852154 NP_010831.1 CDS PAU10 NC_001136.8 1523234 1523596 D Pau10p 1523234..1523596 Saccharomyces cerevisiae 852155 NP_010834.1 CDS YRF1-1 NC_001136.8 1526306 1531696 D Yrf1-1p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 1526306..1531696 Saccharomyces cerevisiae 852158 NP_012092.1 CDS YIL177C NC_001141.1 483 6147 R Yil177cp; Putative protein of unknown function; similarity to DNA helicases that are encoded within subtelomeric Y' elements and induced in telomerase deficient survivors complement(join(483..4598,4987..6147)) Saccharomyces cerevisiae 854630 NP_012093.1 CDS PAU14 NC_001141.1 8793 9155 R Pau14p complement(8793..9155) Saccharomyces cerevisiae 854631 NP_012095.1 CDS VTH1 NC_001141.1 11492 16141 D Vth1p; Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting 11492..16141 Saccharomyces cerevisiae 854634 NP_012096.1 CDS YIL172C NC_001141.1 16784 18553 R Yil172cp; Putative protein of unknown function with similarity to glucosidases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(16784..18553) Saccharomyces cerevisiae 854635 NP_012097.1 CDS YIL169C NC_001141.1 23119 26106 R Yil169cp; Putative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; YIL169C is a non-essential gene complement(23119..26106) Saccharomyces cerevisiae 854637 NP_012100.1 CDS YIL166C NC_001141.1 30938 32566 R Yil166cp; Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene complement(30938..32566) Saccharomyces cerevisiae 854640 NP_012101.1 CDS YIL165C NC_001141.1 33718 34077 R Yil165cp; Putative protein of unknown function; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene complement(33718..34077) Saccharomyces cerevisiae 854641 NP_012102.1 CDS NIT1 NC_001141.1 34087 34686 R Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene; Nit1p complement(34087..34686) Saccharomyces cerevisiae 854642 NP_012104.1 CDS SUC2 NC_001141.1 37385 38983 D Suc2p; Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively 37385..38983 Saccharomyces cerevisiae 854644 NP_012105.1 CDS YIL161W NC_001141.1 39433 40140 D Yil161wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene 39433..40140 Saccharomyces cerevisiae 854645 NP_012106.1 CDS POT1 NC_001141.1 40191 41444 R Pot1p; 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids complement(40191..41444) Saccharomyces cerevisiae 854646 NP_012107.1 CDS BNR1 NC_001141.1 41825 45952 D Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1; Bnr1p 41825..45952 Saccharomyces cerevisiae 854647 NP_012108.1 CDS AIM20 NC_001141.1 46201 46815 D Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim20p 46201..46815 Saccharomyces cerevisiae 854648 NP_012109.1 CDS COA1 NC_001141.1 46949 47542 R Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly; Coa1p complement(46949..47542) Saccharomyces cerevisiae 854649 NP_001027534.1 CDS YIL156W-B NC_001141.1 47690 47973 D Yil156w-bp; Putative protein of unknown function, originally identified based on homology to Ashbya gossypii and other related yeasts join(47690..47698,47761..47973) Saccharomyces cerevisiae 3628034 NP_012110.1 CDS UBP7 NC_001141.1 48091 51306 D Ubp7p; Ubiquitin-specific protease that cleaves ubiquitin-protein fusions 48091..51306 Saccharomyces cerevisiae 854650 NP_012111.1 CDS GUT2 NC_001141.1 51759 53708 R Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner; Gut2p complement(51759..53708) Saccharomyces cerevisiae 854651 NP_012112.1 CDS IMP2' NC_001141.1 53981 55021 R Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat; Imp2'p complement(53981..55021) Saccharomyces cerevisiae 854652 NP_012113.1 CDS RRD1 NC_001141.1 55198 56379 D Rrd1p; Activator of the phosphotyrosyl phosphatase activity of PP2A, peptidyl-prolyl cis/trans-isomerase; regulates G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; subunit of the Tap42p-Sit4p-Rrd1p complex 55198..56379 Saccharomyces cerevisiae 854653 NP_012114.1 CDS YIL152W NC_001141.1 56545 57252 D Yil152wp; Putative protein of unknown function 56545..57252 Saccharomyces cerevisiae 854654 NP_012115.1 CDS YIL151C NC_001141.1 57338 60694 R Yil151cp; Putative protein of unknown function, predicted to contain a PINc (PilT N terminus) domain complement(57338..60694) Saccharomyces cerevisiae 854655 NP_012116.1 CDS MCM10 NC_001141.1 61013 62728 R Mcm10p; Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins complement(61013..62728) Saccharomyces cerevisiae 854656 NP_012117.1 CDS MLP2 NC_001141.1 63028 68067 R Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; Mlp2p complement(63028..68067) Saccharomyces cerevisiae 854657 NP_012118.1 CDS RPL40A NC_001141.1 68708 69528 D Rpl40ap; Fusion protein, identical to Rpl40Bp, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes join(68708..68715,69150..69528) Saccharomyces cerevisiae 854658 NP_012119.1 CDS SLN1 NC_001141.1 69791 73453 R Sln1p; Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators complement(69791..73453) Saccharomyces cerevisiae 854659 NP_012120.1 CDS ECM37 NC_001141.1 74184 75773 R Non-essential protein of unknown function; Ecm37p complement(74184..75773) Saccharomyces cerevisiae 854660 NP_012121.2 CDS PAN6 NC_001141.1 76354 77283 R Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC; Pan6p complement(76354..77283) Saccharomyces cerevisiae 854661 NP_012122.1 CDS TID3 NC_001141.1 78074 80149 D Tid3p; Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering 78074..80149 Saccharomyces cerevisiae 854662 NP_012123.1 CDS SSL2 NC_001141.1 80510 83041 R Ssl2p; Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3 complement(80510..83041) Saccharomyces cerevisiae 854663 NP_012124.1 CDS CCT2 NC_001141.1 83302 84885 D Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct2p 83302..84885 Saccharomyces cerevisiae 854664 NP_012126.1 CDS AXL2 NC_001141.1 85366 87837 D Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate; Axl2p 85366..87837 Saccharomyces cerevisiae 854666 NP_012127.1 CDS REV7 NC_001141.1 87979 88716 R Rev7p; Processivity subunit of DNA polymerase zeta, which is involved in DNA repair and translesion synthesis; required for mutagenesis induced by DNA damage complement(87979..88716) Saccharomyces cerevisiae 854667 NP_012128.1 CDS TPM2 NC_001141.1 89230 89715 R Tpm2p; Minor isoform of tropomyosin, binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p complement(89230..89715) Saccharomyces cerevisiae 854668 NP_012129.1 CDS TMA108 NC_001141.1 89948 92788 R Tma108p; Protein that associates with ribosomes; putative metalloprotease complement(89948..92788) Saccharomyces cerevisiae 854669 NP_012130.1 CDS OM45 NC_001141.1 93619 94800 D Protein of unknown function, major constituent of the mitochondrial outer membrane; located on the outer (cytosolic) face of the outer membrane; Om45p 93619..94800 Saccharomyces cerevisiae 854670 NP_012131.1 CDS VHS2 NC_001141.1 95065 96375 R Vhs2p; Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p complement(95065..96375) Saccharomyces cerevisiae 854671 NP_878096.1 CDS YIL134C-A NC_001141.1 96522 96725 R Yil134c-ap; Putative protein of unknown function, identified by fungal homology and RT-PCR complement(96522..96725) Saccharomyces cerevisiae 1466491 NP_012132.1 CDS FLX1 NC_001141.1 97395 98330 D Protein required for transport of flavin adenine dinucleotide (FAD), a synthesis product of riboflavin, across the mitochondrial membrane; Flx1p 97395..98330 Saccharomyces cerevisiae 854672 NP_012133.1 CDS RPL16A NC_001141.1 98527 99416 R Rpl16ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Bp, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p complement(join(98527..99095,99386..99416)) Saccharomyces cerevisiae 854673 NP_012134.1 CDS CSM2 NC_001141.1 99860 100501 R Protein required for accurate chromosome segregation during meiosis; Csm2p complement(99860..100501) Saccharomyces cerevisiae 854674 NP_012135.1 CDS FKH1 NC_001141.1 100781 102235 R Forkhead family transcription factor with a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; regulates donor preference during switching; Fkh1p complement(100781..102235) Saccharomyces cerevisiae 854675 NP_012136.1 CDS ASG1 NC_001141.1 102782 105676 D Proposed transcriptional activator, member of the Gal4p family of zinc cluster proteins; Asg1p 102782..105676 Saccharomyces cerevisiae 854676 NP_012137.1 CDS TAO3 NC_001141.1 106107 113237 R Tao3p; Protein involved in cell morphogenesis and proliferation, associated with protein kinase Cbk1p; mutants activate OCH1 transcription complement(106107..113237) Saccharomyces cerevisiae 854677 NP_012138.1 CDS MET18 NC_001141.1 113806 116904 D Met18p; DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; involved in telomere maintenance 113806..116904 Saccharomyces cerevisiae 854678 NP_012139.1 CDS YIL127C NC_001141.1 117024 117644 R Yil127cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus complement(117024..117644) Saccharomyces cerevisiae 854679 NP_012140.1 CDS STH1 NC_001141.1 117992 122071 D Sth1p; ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p 117992..122071 Saccharomyces cerevisiae 854680 NP_012141.1 CDS KGD1 NC_001141.1 122689 125733 D Kgd1p; Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA 122689..125733 Saccharomyces cerevisiae 854681 NP_012142.1 CDS AYR1 NC_001141.1 126204 127097 D NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones; Ayr1p 126204..127097 Saccharomyces cerevisiae 854682 NP_012143.1 CDS SIM1 NC_001141.1 128151 129578 D Sim1p; Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated 128151..129578 Saccharomyces cerevisiae 854683 NP_012144.1 CDS POG1 NC_001141.1 130607 131662 D Putative transcriptional activator that promotes recovery from pheromone induced arrest; inhibits both alpha-factor induced G1 arrest and repression of CLN1 and CLN2 via SCB/MCB promoter elements; potential Cdc28p substrate; SBF regulated; Pog1p 130607..131662 Saccharomyces cerevisiae 854684 NP_012145.1 CDS QDR2 NC_001141.1 132241 133869 D Qdr2p; Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin; may have a role in potassium uptake 132241..133869 Saccharomyces cerevisiae 854685 NP_012146.1 CDS QDR1 NC_001141.1 134414 136105 D Qdr1p; Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, ketoconazole, fluconazole, and barban 134414..136105 Saccharomyces cerevisiae 854686 NP_012147.1 CDS RPI1 NC_001141.1 136651 137874 R Rpi1p; Putative transcriptional regulator; overexpression suppresses the heat shock sensitivity of wild-type RAS2 overexpression and also suppresses the cell lysis defect of an mpk1 mutation complement(136651..137874) Saccharomyces cerevisiae 854687 NP_012148.1 CDS RHO3 NC_001141.1 139749 140444 D Rho3p; Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p 139749..140444 Saccharomyces cerevisiae 854688 NP_012149.1 CDS PRM5 NC_001141.1 140610 141566 R Prm5p; Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling complement(140610..141566) Saccharomyces cerevisiae 854689 NP_012150.1 CDS HIS5 NC_001141.1 142925 144082 D Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; His5p 142925..144082 Saccharomyces cerevisiae 854690 NP_012151.1 CDS NUP159 NC_001141.1 144324 148706 R Nup159p; Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, forms a subcomplex with Nup82p and Nsp1p, required for mRNA export complement(144324..148706) Saccharomyces cerevisiae 854691 NP_012152.1 CDS POR2 NC_001141.1 149140 149985 R Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane permeability or mitochondrial osmotic stability; Por2p complement(149140..149985) Saccharomyces cerevisiae 854692 NP_012153.1 CDS SDP1 NC_001141.1 150556 151185 D Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; Sdp1p 150556..151185 Saccharomyces cerevisiae 854693 NP_012154.1 CDS HOS4 NC_001141.1 151592 154843 D Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate; Hos4p 151592..154843 Saccharomyces cerevisiae 854694 NP_012155.1 CDS COX5B NC_001141.1 155219 155762 D Subunit Vb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; Cox5bp join(155219,155308..155762) Saccharomyces cerevisiae 854695 NP_012156.1 CDS MNI1 NC_001141.1 156042 157175 D Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; deletion mutant exhibits a weak vacuolar protein sorting defect, enhanced resistance to caspofungin, and is synthetically lethal with MEN mutants; Mni1p 156042..157175 Saccharomyces cerevisiae 854696 NP_012157.1 CDS SEC24 NC_001141.1 157382 160162 R Sec24p; Component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat; involved in ER to Golgi transport, cargo selection and autophagy; required for the binding of the Sec13 complex to ER membranes; homologous to Lst1p and Lss1p complement(157382..160162) Saccharomyces cerevisiae 854697 NP_012158.1 CDS YIL108W NC_001141.1 160884 162974 D Yil108wp; Putative metalloprotease 160884..162974 Saccharomyces cerevisiae 854698 NP_012159.1 CDS PFK26 NC_001141.1 163275 165758 R Pfk26p; 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, has negligible fructose-2,6-bisphosphatase activity, transcriptional regulation involves protein kinase A complement(163275..165758) Saccharomyces cerevisiae 854699 NP_012160.2 CDS MOB1 NC_001141.1 166412 167441 D Mob1p; Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex join(166412..166431,166517..167441) Saccharomyces cerevisiae 854700 NP_012161.1 CDS SLM1 NC_001141.1 167578 169638 R Slm1p; Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex complement(167578..169638) Saccharomyces cerevisiae 854701 NP_012162.1 CDS SHQ1 NC_001141.1 169979 171502 R Shq1p; Essential nuclear protein, required for accumulation of box H/ACA snoRNAs and for rRNA processing; interacts with Naf1p complement(169979..171502) Saccharomyces cerevisiae 854702 NP_012163.1 CDS DPH1 NC_001141.1 171748 173025 D Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p; Dph1p 171748..173025 Saccharomyces cerevisiae 854703 NP_001032579.1 CDS YIL102C-A NC_001141.1 173362 173589 R Yil102c-ap; Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species complement(173362..173589) Saccharomyces cerevisiae 3799974 NP_012164.1 CDS YIL102C NC_001141.1 174579 174884 R Yil102cp; Putative protein of unknown function complement(174579..174884) Saccharomyces cerevisiae 854704 NP_012165.1 CDS XBP1 NC_001141.1 175304 177247 R Xbp1p; Transcriptional repressor that binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate complement(175304..177247) Saccharomyces cerevisiae 854706 NP_012167.1 CDS SGA1 NC_001141.1 178001 179650 D Sga1p; Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation 178001..179650 Saccharomyces cerevisiae 854708 NP_012168.1 CDS FMC1 NC_001141.1 179769 180236 R Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p; Fmc1p complement(179769..180236) Saccharomyces cerevisiae 854709 NP_012169.1 CDS FYV10 NC_001141.1 180424 181974 D Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains CTLH domain; Fyv10p 180424..181974 Saccharomyces cerevisiae 854710 NP_012170.1 CDS YIL096C NC_001141.1 182114 183124 R Yil096cp; Putative protein of unknown function; associates with precursors of the 60S ribosomal subunit complement(182114..183124) Saccharomyces cerevisiae 854711 NP_012171.1 CDS PRK1 NC_001141.1 183934 186366 D Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; Prk1p 183934..186366 Saccharomyces cerevisiae 854713 NP_012172.1 CDS LYS12 NC_001141.1 186514 187629 R Lys12p; Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate complement(186514..187629) Saccharomyces cerevisiae 854714 NP_012173.1 CDS RSM25 NC_001141.1 187987 188781 R Rsm25p; Mitochondrial ribosomal protein of the small subunit complement(187987..188781) Saccharomyces cerevisiae 854715 NP_012174.1 CDS YIL092W NC_001141.1 189063 190964 D Yil092wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 189063..190964 Saccharomyces cerevisiae 854716 NP_012175.1 CDS YIL091C NC_001141.1 191027 193192 R Yil091cp; Protein required for cell viability complement(191027..193192) Saccharomyces cerevisiae 854717 NP_012176.1 CDS ICE2 NC_001141.1 193592 195067 D Integral ER membrane protein with type-III transmembrane domains; mutations cause defects in cortical ER morphology in both the mother and daughter cells; Ice2p 193592..195067 Saccharomyces cerevisiae 854718 NP_012177.1 CDS YIL089W NC_001141.1 195596 196213 D Yil089wp; Putative protein of unknown function 195596..196213 Saccharomyces cerevisiae 854719 NP_012178.1 CDS AVT7 NC_001141.1 197928 199400 R Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt7p complement(197928..199400) Saccharomyces cerevisiae 854721 NP_012179.1 CDS LRC2 NC_001141.1 199643 200116 R Putative protein of unknown function; protein is detected in purified mitochondria in high-throughput studies; null mutant displays decreased mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Lrc2p complement(199643..200116) Saccharomyces cerevisiae 854722 NP_012181.1 CDS KTR7 NC_001141.1 200487 202040 R Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Ktr7p complement(200487..202040) Saccharomyces cerevisiae 854724 NP_012182.1 CDS SDS3 NC_001141.1 202273 203256 R Sds3p; Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes complement(202273..203256) Saccharomyces cerevisiae 854725 NP_012183.1 CDS YIL083C NC_001141.1 203553 204650 R Yil083cp; Putative phosphopantothenoylcysteine synthetase (PPCS) that catalyzes the second step in the biosynthesis of coenzyme A from pantothenate; conserved in bacteria, humans, and plants; essential for viability complement(203553..204650) Saccharomyces cerevisiae 854726 NP_012184.1 CDS YIL082W-A NC_001141.1 205632 210129 D Yil082w-ap; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(205632..206482,206484..210129) Saccharomyces cerevisiae 854728 NP_012186.1 CDS AIR1 NC_001141.1 210920 212002 R RING finger protein that interacts with the arginine methyltransferase Hmt1p to regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air2p; Air1p complement(210920..212002) Saccharomyces cerevisiae 854731 NP_116578.1 CDS THS1 NC_001141.1 212496 214700 D Ths1p; Threonyl-tRNA synthetase, essential cytoplasmic protein 212496..214700 Saccharomyces cerevisiae 854732 NP_012188.1 CDS YIL077C NC_001141.1 214988 215950 R Yil077cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) complement(214988..215950) Saccharomyces cerevisiae 854733 NP_012189.2 CDS SEC28 NC_001141.1 216655 217545 D Sec28p; Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth 216655..217545 Saccharomyces cerevisiae 854734 NP_012190.1 CDS RPN2 NC_001141.1 217860 220697 R Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p; Rpn2p complement(217860..220697) Saccharomyces cerevisiae 854735 NP_012191.1 CDS SER33 NC_001141.1 221078 222487 R Ser33p; 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser3p complement(221078..222487) Saccharomyces cerevisiae 854736 NP_012192.2 CDS SPO22 NC_001141.1 222934 225951 R Spo22p; Meiosis-specific protein essential for chromosome synapsis, similar to phospholipase A2, involved in completion of nuclear divisions during meiosis; induced early in meiosis complement(join(222934..225806,225897..225951)) Saccharomyces cerevisiae 854737 NP_012193.1 CDS HOP1 NC_001141.1 226599 228416 D Meiosis-specific DNA binding protein that displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for homologous chromosome synapsis and chiasma formation; Hop1p 226599..228416 Saccharomyces cerevisiae 854738 NP_085069.1 CDS PCI8 NC_001141.1 228657 229991 R Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain; Pci8p complement(228657..229991) Saccharomyces cerevisiae 854739 NP_012194.1 CDS MAM33 NC_001141.1 230269 231069 R Mam33p; Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R complement(230269..231069) Saccharomyces cerevisiae 854740 NP_012195.1 CDS RPS24B NC_001141.1 231550 232366 R Rps24bp; Protein component of the small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein complement(join(231550..231954,232364..232366)) Saccharomyces cerevisiae 854741 NP_012196.1 CDS SEC6 NC_001141.1 233054 235471 R Sec6p; Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesicles to active sites of exocytosis; dimeric form of Sec6p interacts with Sec9p in vitro and inhibits t-SNARE assembly complement(233054..235471) Saccharomyces cerevisiae 854742 NP_012197.1 CDS YIL067C NC_001141.1 235721 237757 R Yil067cp; Uncharacterized protein of unknown function complement(235721..237757) Saccharomyces cerevisiae 854743 NP_012198.1 CDS RNR3 NC_001141.1 238096 240705 R Rnr3p; Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits complement(238096..240705) Saccharomyces cerevisiae 854744 NP_012199.1 CDS FIS1 NC_001141.1 241305 241772 R Mitochondrial outer membrane protein involved in membrane fission, required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation; Fis1p complement(241305..241772) Saccharomyces cerevisiae 854745 NP_012200.1 CDS YIL064W NC_001141.1 241940 242713 D Yil064wp; Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family 241940..242713 Saccharomyces cerevisiae 854746 NP_012201.1 CDS YRB2 NC_001141.1 242758 243741 R Yrb2p; Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential complement(242758..243741) Saccharomyces cerevisiae 854747 NP_012202.1 CDS ARC15 NC_001141.1 243995 244459 R Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; Arc15p complement(243995..244459) Saccharomyces cerevisiae 854748 NP_012203.1 CDS SNP1 NC_001141.1 244654 245556 R Snp1p; Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein complement(244654..245556) Saccharomyces cerevisiae 854749 NP_012204.1 CDS YIL060W NC_001141.1 246389 246823 D Yil060wp; Putative protein of unknown function; mutant accumulates less glycogen than does wild type; YIL060W is not an essential gene 246389..246823 Saccharomyces cerevisiae 854750 NP_012207.1 CDS YIL057C NC_001141.1 247899 248393 R Yil057cp; Putative protein of unknown function; expression induced under carbon limitation and repressed under high glucose complement(247899..248393) Saccharomyces cerevisiae 854753 NP_012208.1 CDS VHR1 NC_001141.1 249989 251911 D Vhr1p; Transcriptional activator, required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations 249989..251911 Saccharomyces cerevisiae 854755 NP_012209.1 CDS YIL055C NC_001141.1 252040 253923 R Yil055cp; Putative protein of unknown function complement(252040..253923) Saccharomyces cerevisiae 854756 NP_012211.2 CDS RHR2 NC_001141.1 255113 255865 D Rhr2p; Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress 255113..255865 Saccharomyces cerevisiae 854758 NP_012212.1 CDS RPL34B NC_001141.1 256224 257061 R Rpl34bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Ap and has similarity to rat L34 ribosomal protein complement(join(256224..256552,257025..257061)) Saccharomyces cerevisiae 854759 NP_012213.1 CDS MMF1 NC_001141.1 257842 258279 R Mmf1p; Mitochondrial protein involved in maintenance of the mitochondrial genome complement(257842..258279) Saccharomyces cerevisiae 854760 NP_012214.1 CDS PCL7 NC_001141.1 258912 259769 D Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; Pcl7p 258912..259769 Saccharomyces cerevisiae 854761 NP_012215.1 CDS DFG10 NC_001141.1 260157 260918 D Protein of unknown function, involved in filamentous growth; Dfg10p 260157..260918 Saccharomyces cerevisiae 854762 NP_012216.1 CDS NEO1 NC_001141.1 261436 264891 D Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus; Neo1p 261436..264891 Saccharomyces cerevisiae 854763 NP_012217.1 CDS SYG1 NC_001141.1 265114 267822 R Syg1p; Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency complement(265114..267822) Saccharomyces cerevisiae 854764 NP_878097.1 CDS YIL046W-A NC_001141.1 268308 268472 D Yil046w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 268308..268472 Saccharomyces cerevisiae 1466492 NP_012218.1 CDS MET30 NC_001141.1 268650 270572 D Met30p; F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus 268650..270572 Saccharomyces cerevisiae 854765 NP_012219.1 CDS PIG2 NC_001141.1 271160 272776 D Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; Pig2p 271160..272776 Saccharomyces cerevisiae 854766 NP_012220.1 CDS AGE2 NC_001141.1 272949 273845 R Age2p; ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif complement(272949..273845) Saccharomyces cerevisiae 854767 NP_012221.2 CDS CBR1 NC_001141.1 274071 274925 R Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia; Cbr1p complement(274071..274925) Saccharomyces cerevisiae 854768 NP_012222.1 CDS PKP1 NC_001141.1 275107 276291 R Pkp1p; Mitochondrial kinase, phosphorylates pyruvate dehydrogenase alpha subunit Pda1p complement(275107..276291) Saccharomyces cerevisiae 854769 NP_012223.1 CDS GVP36 NC_001141.1 276524 277504 D Gvp36p; Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles; may interact with ribosomes, based on co-purification experiments 276524..277504 Saccharomyces cerevisiae 854770 NP_012224.1 CDS APQ12 NC_001141.1 277722 278138 D Protein involved in nucleocytoplasmic transport of mRNA; Apq12p 277722..278138 Saccharomyces cerevisiae 854771 NP_012225.1 CDS TED1 NC_001141.1 278425 279846 D Ted1p; Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) 278425..279846 Saccharomyces cerevisiae 854772 NP_012226.1 CDS NOT3 NC_001141.1 280141 282651 R Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation; Not3p complement(280141..282651) Saccharomyces cerevisiae 854773 NP_012227.1 CDS PRM2 NC_001141.1 283028 284998 R Prm2p; Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p complement(283028..284998) Saccharomyces cerevisiae 854774 NP_012228.1 CDS CST6 NC_001141.1 285665 287428 D Cst6p; Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, activates transcription of genes involved in utilization of non-optimal carbon sources; involved in telomere maintenance 285665..287428 Saccharomyces cerevisiae 854775 NP_012229.1 CDS CKA1 NC_001141.1 287789 288907 R Cka1p; Alpha catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA2, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases complement(287789..288907) Saccharomyces cerevisiae 854776 NP_012230.1 CDS CAP2 NC_001141.1 289225 290088 R Cap2p; Beta subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches complement(289225..290088) Saccharomyces cerevisiae 854777 NP_012231.1 CDS BCY1 NC_001141.1 290418 291668 R Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation; Bcy1p complement(290418..291668) Saccharomyces cerevisiae 854778 NP_012233.1 CDS ULP2 NC_001141.1 292632 295736 D Ulp2p; Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate 292632..295736 Saccharomyces cerevisiae 854780 NP_012234.1 CDS SSM4 NC_001141.1 296049 300008 R Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation; Ssm4p complement(296049..300008) Saccharomyces cerevisiae 854781 NP_012235.1 CDS YIL029C NC_001141.1 300827 301255 R Yil029cp; Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) complement(300827..301255) Saccharomyces cerevisiae 854783 NP_012237.1 CDS KRE27 NC_001141.1 303677 304102 R Protein of unknown function; null mutant shows K1 killer toxin resistance; Kre27p complement(303677..304102) Saccharomyces cerevisiae 854785 NP_012238.1 CDS IRR1 NC_001141.1 304475 307927 R Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability; Irr1p complement(304475..307927) Saccharomyces cerevisiae 854786 NP_012240.1 CDS YIL024C NC_001141.1 308600 309169 R Yil024cp; Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p complement(308600..309169) Saccharomyces cerevisiae 854788 NP_012241.1 CDS YKE4 NC_001141.1 309384 310424 R Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family; Yke4p complement(309384..310424) Saccharomyces cerevisiae 854789 NP_012242.1 CDS TIM44 NC_001141.1 311163 312458 D Tim44p; Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane 311163..312458 Saccharomyces cerevisiae 854790 NP_012243.1 CDS RPB3 NC_001141.1 312903 313859 D Rpb3p; RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit 312903..313859 Saccharomyces cerevisiae 854791 NP_012244.1 CDS HIS6 NC_001141.1 314033 314818 R Phosphoribosyl-5-amino-1-phosphoribosyl-4- imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; His6p complement(314033..314818) Saccharomyces cerevisiae 854792 NP_012245.1 CDS FAF1 NC_001141.1 315091 316131 D Protein required for pre-rRNA processing and 40S ribosomal subunit assembly; Faf1p 315091..316131 Saccharomyces cerevisiae 854793 NP_012246.1 CDS RPL2B NC_001141.1 316766 317930 D Rpl2bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures join(316766..316769,317170..317930) Saccharomyces cerevisiae 854794 NP_012247.1 CDS VID28 NC_001141.1 318198 320963 R Vid28p; Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm complement(318198..320963) Saccharomyces cerevisiae 854795 NP_012248.1 CDS SNL1 NC_001141.1 321452 321931 D Snl1p; Protein of unknown function proposed to be involved in nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein 321452..321931 Saccharomyces cerevisiae 854796 NP_012249.1 CDS BAR1 NC_001141.1 322340 324103 D Bar1p; Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest 322340..324103 Saccharomyces cerevisiae 854797 NP_012250.1 CDS YIL014C-A NC_001141.1 325210 325524 R Yil014c-ap; Putative protein of unknown function complement(325210..325524) Saccharomyces cerevisiae 854799 NP_012251.1 CDS MNT3 NC_001141.1 326101 327993 D Mnt3p; Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation 326101..327993 Saccharomyces cerevisiae 854801 NP_012252.1 CDS PDR11 NC_001141.1 328205 332440 R Pdr11p; ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromisedregulated by Pdr1p; required for anaerobic growth complement(328205..332440) Saccharomyces cerevisiae 854802 NP_012254.1 CDS TIR3 NC_001141.1 333724 334533 D Tir3p; Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth 333724..334533 Saccharomyces cerevisiae 854804 NP_012255.1 CDS DOT5 NC_001141.1 334879 335526 D Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth; Dot5p 334879..335526 Saccharomyces cerevisiae 854805 NP_012256.1 CDS EST3 NC_001141.1 335663 336209 R Est3p; Component of the telomerase holoenzyme, involved in telomere replication complement(join(335663..335932,335934..336209)) Saccharomyces cerevisiae 854806 NP_012257.1 CDS FAA3 NC_001141.1 339341 341425 D Long chain fatty acyl-CoA synthetase, has a preference for C16 and C18 fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; Faa3p 339341..341425 Saccharomyces cerevisiae 854808 NP_012258.1 CDS URM1 NC_001141.1 342533 342832 D Urm1p; Ubiquitin-like protein with weak sequence similarity to ubiquitin; depends on the E1-like activating enzyme Uba4p; molecular function of the Urm1p pathway is unknown, but it is required for normal growth, particularly at high temperature 342533..342832 Saccharomyces cerevisiae 854809 NP_012259.1 CDS NAS2 NC_001141.1 342991 343653 R Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p; Nas2p complement(342991..343653) Saccharomyces cerevisiae 854810 NP_012260.1 CDS YIA6 NC_001141.1 344059 345180 D Yia6p; Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs 344059..345180 Saccharomyces cerevisiae 854811 NP_012261.1 CDS EPS1 NC_001141.1 345689 347794 D Eps1p; ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family 345689..347794 Saccharomyces cerevisiae 854812 NP_012262.1 CDS BET1 NC_001141.1 347943 348502 R Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins; Bet1p complement(join(347943..348360,348492..348502)) Saccharomyces cerevisiae 854813 NP_012263.1 CDS CFD1 NC_001141.1 349119 350000 D Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol; Cfd1p 349119..350000 Saccharomyces cerevisiae 854814 NP_878098.1 CDS YIL002W-A NC_001141.1 350298 350507 D Yil002w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 350298..350507 Saccharomyces cerevisiae 1466493 NP_012264.1 CDS INP51 NC_001141.1 350588 353428 R Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth; Inp51p complement(350588..353428) Saccharomyces cerevisiae 854815 NP_012265.1 CDS YIL001W NC_001141.1 353937 355478 D Yil001wp 353937..355478 Saccharomyces cerevisiae 854816 NP_012266.1 CDS SGN1 NC_001141.1 356140 356892 R Sgn1p; Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation complement(356140..356892) Saccharomyces cerevisiae 854817 NP_012267.1 CDS MPH1 NC_001141.1 357412 360393 R Mph1p; Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype complement(357412..360393) Saccharomyces cerevisiae 854818 NP_012268.1 CDS AIM21 NC_001141.1 360882 362921 D Protein of unknown function; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim21p 360882..362921 Saccharomyces cerevisiae 854819 NP_012269.1 CDS DJP1 NC_001141.1 363218 364516 D Djp1p; Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ 363218..364516 Saccharomyces cerevisiae 854820 NP_012270.1 CDS IST3 NC_001141.1 364886 365332 D Ist3p; Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing 364886..365332 Saccharomyces cerevisiae 854821 NP_012271.1 CDS PAN1 NC_001141.1 365463 369905 R Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease; Pan1p complement(365463..369905) Saccharomyces cerevisiae 854822 NP_012272.1 CDS YIR007W NC_001141.1 370701 372995 D Yir007wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene 370701..372995 Saccharomyces cerevisiae 854824 NP_012273.1 CDS PRI1 NC_001141.1 373074 374303 R Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair; Pri1p complement(373074..374303) Saccharomyces cerevisiae 854825 NP_012274.1 CDS MSL1 NC_001141.1 374522 374857 D U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro; does not contain the conserved C-terminal RNA binding domain found in other family members; Msl1p 374522..374857 Saccharomyces cerevisiae 854826 NP_012275.1 CDS DSN1 NC_001141.1 375428 377158 D Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation; Dsn1p 375428..377158 Saccharomyces cerevisiae 854827 NP_012276.1 CDS STS1 NC_001141.1 377284 378243 R Sts1p; Protein that interacts with the karyopherin Srp1p; may have a role with Srp1p in ubiquitin-mediated protein degradation complement(377284..378243) Saccharomyces cerevisiae 854828 NP_012277.1 CDS SQT1 NC_001141.1 378483 379778 D Sqt1p; Essential protein involved in a late step of 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay 378483..379778 Saccharomyces cerevisiae 854829 NP_012278.1 CDS GAT4 NC_001141.1 380016 380381 R Protein containing GATA family zinc finger motifs; Gat4p complement(380016..380381) Saccharomyces cerevisiae 854830 NP_012279.2 CDS YIR014W NC_001141.1 381083 381811 D Yir014wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene 381083..381811 Saccharomyces cerevisiae 854831 NP_012280.1 CDS RPR2 NC_001141.1 381945 382379 D Subunit of nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; Rpr2p 381945..382379 Saccharomyces cerevisiae 854832 NP_012281.1 CDS YIR016W NC_001141.1 382625 383422 D Yir016wp; Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR016W is a non-essential gene 382625..383422 Saccharomyces cerevisiae 854833 NP_012282.1 CDS MET28 NC_001141.1 383553 384116 R Transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism; Met28p complement(383553..384116) Saccharomyces cerevisiae 854834 NP_012283.1 CDS YAP5 NC_001141.1 384606 385343 D Yap5p; Basic leucine zipper (bZIP) transcription factor 384606..385343 Saccharomyces cerevisiae 854835 NP_878099.1 CDS YIR018C-A NC_001141.1 385561 385698 R Yir018c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(385561..385698) Saccharomyces cerevisiae 1466494 NP_012284.1 CDS MUC1 NC_001141.1 389569 393672 R Muc1p; GPI-anchored cell surface glycoprotein (flocculin) required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p) complement(389569..393672) Saccharomyces cerevisiae 854836 NP_012287.1 CDS MRS1 NC_001141.1 397291 398382 D Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; Mrs1p 397291..398382 Saccharomyces cerevisiae 854839 NP_878100.1 CDS YIR021W-A NC_001141.1 398511 398723 D Yir021w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 398511..398723 Saccharomyces cerevisiae 1466495 NP_012288.1 CDS SEC11 NC_001141.1 398730 399233 D Sec11p; 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum 398730..399233 Saccharomyces cerevisiae 854840 NP_012289.1 CDS DAL81 NC_001141.1 399774 402686 D Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism; Dal81p 399774..402686 Saccharomyces cerevisiae 854841 NP_012290.1 CDS YIR024C NC_001141.1 402838 403488 R Yir024cp; Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect complement(402838..403488) Saccharomyces cerevisiae 854842 NP_012291.1 CDS MND2 NC_001141.1 403656 404762 D Subunit of the anaphase-promoting complex (APC); needed for meiotic nuclear division; Mnd2p 403656..404762 Saccharomyces cerevisiae 854843 NP_012292.1 CDS YVH1 NC_001141.1 404870 405964 R Yvh1p; Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; transcription induced by low temperature and nitrogen starvation; member of the dual-specificity family of protein phosphatases complement(404870..405964) Saccharomyces cerevisiae 854844 NP_012293.1 CDS DAL1 NC_001141.1 406257 407639 R Dal1p; Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression complement(406257..407639) Saccharomyces cerevisiae 854845 NP_012294.1 CDS DAL4 NC_001141.1 408465 410372 D Dal4p; Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation 408465..410372 Saccharomyces cerevisiae 854846 NP_012295.1 CDS DAL2 NC_001141.1 410804 411835 D Dal2p; Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation 410804..411835 Saccharomyces cerevisiae 854847 NP_012296.1 CDS DCG1 NC_001141.1 412033 412767 R Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain; Dcg1p complement(412033..412767) Saccharomyces cerevisiae 854848 NP_012297.1 CDS DAL7 NC_001141.1 413012 414676 R Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; Dal7p complement(413012..414676) Saccharomyces cerevisiae 854849 NP_012298.1 CDS DAL3 NC_001141.1 415027 415614 R Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression; Dal3p complement(415027..415614) Saccharomyces cerevisiae 854850 NP_012299.1 CDS MGA2 NC_001141.1 416121 419462 D Mga2p; ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting 416121..419462 Saccharomyces cerevisiae 854851 NP_012300.1 CDS LYS1 NC_001141.1 419612 420733 R Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; Lys1p complement(419612..420733) Saccharomyces cerevisiae 854852 NP_012301.1 CDS YIR035C NC_001141.1 421023 421787 R Yir035cp; Putative cytoplasmic protein of unknown function complement(421023..421787) Saccharomyces cerevisiae 854853 NP_012302.1 CDS IRC24 NC_001141.1 422071 422862 R Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52 foci; Irc24p complement(422071..422862) Saccharomyces cerevisiae 854854 NP_012303.1 CDS HYR1 NC_001141.1 423125 423616 D Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; Hyr1p 423125..423616 Saccharomyces cerevisiae 854855 NP_012304.1 CDS GTT1 NC_001141.1 423806 424510 R Gtt1p; ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p complement(423806..424510) Saccharomyces cerevisiae 854856 NP_012305.1 CDS YPS6 NC_001141.1 430494 432107 R Yps6p; Putative GPI-anchored aspartic protease complement(430494..432107) Saccharomyces cerevisiae 854857 NP_012307.1 CDS PAU15 NC_001141.1 433925 434299 D Pau15p 433925..434299 Saccharomyces cerevisiae 854859 NP_012308.1 CDS YIR042C NC_001141.1 435270 435980 R Yir042cp; Putative protein of unknown function; YIR042C is a non-essential gene complement(435270..435980) Saccharomyces cerevisiae 854860 NP_009305.1 CDS COX1 NC_001224.1 13818 26701 D Subunit I of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits; Cox1p join(13818..13986,16435..16470,18954..18991,20508..20984,21995..22246,23612..23746,25318..25342,26229..26701) Saccharomyces cerevisiae 854598 NP_009306.1 CDS AI5_ALPHA NC_001224.1 13818 23167 D Endonuclease I-SceIV, involved in intron mobility; encoded by a mobile group I intron within the mitochondrial COX1 gene; Ai5_alphap join(13818..13986,16435..16470,18954..18991,20508..20984,21995..23167) Saccharomyces cerevisiae 854597 NP_009307.2 CDS AI4 NC_001224.1 13818 21935 D Endonuclease I-SceII, encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity; Ai4p join(13818..13986,16435..16470,18954..18991,20508..21935) Saccharomyces cerevisiae 854596 NP_009308.2 CDS AI3 NC_001224.1 13818 19996 D Endonuclease I-SceIII, encoded by a mobile group I intron within the mitochondrial COX1 gene; Ai3p join(13818..13986,16435..16470,18954..19996) Saccharomyces cerevisiae 854595 NP_009309.1 CDS AI2 NC_001224.1 13818 18830 D Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene; Ai2p join(13818..13986,16435..18830) Saccharomyces cerevisiae 854594 NP_009310.1 CDS AI1 NC_001224.1 13818 16322 D Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene; Ai1p 13818..16322 Saccharomyces cerevisiae 854593 NP_009311.2 CDS AI5_BETA NC_001224.1 24156 25255 D Protein of unknown function, encoded within an intron of the mitochondrial COX1 gene; translational initiation codon is predicted to be ATA rather than ATG; Ai5_betap join(24156..24870,24906..25255) Saccharomyces cerevisiae 854599 NP_009312.1 CDS ATP8 NC_001224.1 27666 27812 D Subunit 8 of the F0 sector of mitochondrial inner membrane F1-F0 ATP synthase, encoded on the mitochondrial genome; Atp8p 27666..27812 Saccharomyces cerevisiae 854600 NP_009313.1 CDS ATP6 NC_001224.1 28487 29266 D Mitochondrially encoded subunit 6 of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp6p 28487..29266 Saccharomyces cerevisiae 854601 NP_009315.1 CDS COB NC_001224.1 36540 43647 D Cytochrome b, mitochondrially encoded subunit of the ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p; Cobp join(36540..36954,37723..37736,39141..39217,40841..41090,42508..42558,43297..43647) Saccharomyces cerevisiae 854583 NP_009316.1 CDS BI4 NC_001224.1 36540 42251 D Mitochondrial mRNA maturase, forms a complex with Nam2p to mediate splicing of the bI4 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA; Bi4p join(36540..36954,37723..37736,39141..39217,40841..42251) Saccharomyces cerevisiae 854582 NP_009317.1 CDS BI3 NC_001224.1 36540 40265 D Mitochondrial mRNA maturase, forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA; Bi3p join(36540..36954,37723..37736,39141..40265) Saccharomyces cerevisiae 854605 NP_009318.1 CDS BI2 NC_001224.1 36540 38579 D Mitochondrial mRNA maturase with a role in splicing, encoded by both exon and intron sequences of partially processed COB mRNA; Bi2p join(36540..36954,37723..38579) Saccharomyces cerevisiae 854604 NP_009319.1 CDS OLI1 NC_001224.1 46723 46953 D Oli1p; F0-ATP synthase subunit 9 (ATPase-associated proteolipid), encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2 46723..46953 Saccharomyces cerevisiae 854584 NP_009320.1 CDS VAR1 NC_001224.1 48901 50097 D Var1p; Mitochondrial ribosomal protein of the small subunit, mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane 48901..50097 Saccharomyces cerevisiae 854586 NP_009324.1 CDS SCEI NC_001224.1 61022 61729 D Sceip; I-SceI DNA endonuclease, encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene 61022..61729 Saccharomyces cerevisiae 854590 NP_009326.1 CDS COX2 NC_001224.1 73758 74513 D Subunit II of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits; Cox2p 73758..74513 Saccharomyces cerevisiae 854622 NP_009327.1 CDS Q0255 NC_001224.1 74495 75984 D Q0255p; Maturase-like protein join(74495..75622,75663..75872,75904..75984) Saccharomyces cerevisiae 854623 NP_009328.1 CDS COX3 NC_001224.1 79213 80022 D Subunit III of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits; Cox3p 79213..80022 Saccharomyces cerevisiae 854627 NP_010835.1 CDS YEL077C NC_001137.2 264 4097 R Yel077cp; Helicase-like protein encoded within the telomeric Y' element complement(264..4097) Saccharomyces cerevisiae 856630 NP_010836.1 CDS YEL076C-A NC_001137.2 4185 5114 R Yel076c-ap; Putative protein of unknown function complement(join(4185..4322,4602..5114)) Saccharomyces cerevisiae 856632 NP_010837.1 CDS YEL076C NC_001137.2 4464 5114 R Yel076cp; Putative protein of unknown function complement(4464..5114) Saccharomyces cerevisiae 856633 NP_010839.1 CDS YEL075C NC_001137.2 5345 5713 R Yel075cp; Putative protein of unknown function complement(5345..5713) Saccharomyces cerevisiae 856634 NP_010841.1 CDS YEL073C NC_001137.2 7230 7553 R Yel073cp; Putative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline complement(7230..7553) Saccharomyces cerevisiae 856636 NP_010842.1 CDS RMD6 NC_001137.2 13720 14415 D Rmd6p; Protein required for sporulation 13720..14415 Saccharomyces cerevisiae 856637 NP_010843.1 CDS DLD3 NC_001137.2 16355 17845 D Dld3p; D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm 16355..17845 Saccharomyces cerevisiae 856638 NP_010844.1 CDS DSF1 NC_001137.2 19589 21097 D Dsf1p; Deletion suppressor of mpt5 mutation 19589..21097 Saccharomyces cerevisiae 856639 NP_010845.1 CDS HXT13 NC_001137.2 21537 23231 R Hxt13p; Hexose transporter, induced in the presence of non-fermentable carbon sources, induced by low levels of glucose, repressed by high levels of glucose complement(21537..23231) Saccharomyces cerevisiae 856640 NP_010847.1 CDS YEL067C NC_001137.2 26189 26776 R Yel067cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(26189..26776) Saccharomyces cerevisiae 856643 NP_010848.1 CDS HPA3 NC_001137.2 26667 27206 D Hpa3p; D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro 26667..27206 Saccharomyces cerevisiae 856642 NP_010849.1 CDS SIT1 NC_001137.2 27657 29543 D Sit1p; Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p 27657..29543 Saccharomyces cerevisiae 856644 NP_010850.1 CDS AVT2 NC_001137.2 29797 31239 R Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt2p complement(29797..31239) Saccharomyces cerevisiae 856645 NP_010851.1 CDS CAN1 NC_001137.2 31694 33466 R Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; Can1p complement(31694..33466) Saccharomyces cerevisiae 856646 NP_010852.2 CDS NPR2 NC_001137.2 34407 36254 D Protein with a possible role in regulating expression of nitrogen permeases; transcription is induced in response to proline and urea; contains two PEST sequences; null mutant is resistant to cisplatin and doxorubicin; Npr2p 34407..36254 Saccharomyces cerevisiae 856647 NP_010853.2 CDS CIN8 NC_001137.2 36535 39537 R Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation; Cin8p complement(36535..39537) Saccharomyces cerevisiae 856648 NP_010854.1 CDS PRB1 NC_001137.2 40046 41953 R Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; Prb1p complement(40046..41953) Saccharomyces cerevisiae 856649 NP_058154.1 CDS SOM1 NC_001137.2 42400 42624 R Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; Som1p complement(42400..42624) Saccharomyces cerevisiae 856650 NP_010856.1 CDS PCM1 NC_001137.2 43252 44925 D Pcm1p; Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors 43252..44925 Saccharomyces cerevisiae 856652 NP_010857.1 CDS YEL057C NC_001137.2 45020 45721 R Yel057cp; Protein of unknown function involved in telomere maintenance; target of UME6 regulation complement(45020..45721) Saccharomyces cerevisiae 856653 NP_010858.1 CDS HAT2 NC_001137.2 47168 48373 D Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing; Hat2p 47168..48373 Saccharomyces cerevisiae 856654 NP_010859.1 CDS POL5 NC_001137.2 48471 51539 R Pol5p; DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA complement(48471..51539) Saccharomyces cerevisiae 856655 NP_010860.1 CDS RPL12A NC_001137.2 52721 53218 R Rpl12ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins complement(52721..53218) Saccharomyces cerevisiae 856656 NP_010861.1 CDS MAK10 NC_001137.2 53901 56102 R Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible; Mak10p complement(53901..56102) Saccharomyces cerevisiae 856657 NP_010862.1 CDS AFG1 NC_001137.2 56571 58100 D Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain; Afg1p 56571..58100 Saccharomyces cerevisiae 856658 NP_010863.1 CDS VMA8 NC_001137.2 58378 59148 D Vma8p; Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis 58378..59148 Saccharomyces cerevisiae 856659 NP_010864.1 CDS RML2 NC_001137.2 59670 60851 R Rml2p; Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor complement(59670..60851) Saccharomyces cerevisiae 856660 NP_010865.1 CDS PAU2 NC_001137.2 63728 64090 D Pau2p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme 63728..64090 Saccharomyces cerevisiae 856662 NP_010866.1 CDS YEL048C NC_001137.2 64709 65167 R Yel048cp; Putative protein of unknown function; synthetic lethal with gcs1delta complement(64709..65167) Saccharomyces cerevisiae 856663 NP_010867.1 CDS YEL047C NC_001137.2 65385 66797 R Yel047cp; Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies complement(65385..66797) Saccharomyces cerevisiae 856664 NP_010868.1 CDS GLY1 NC_001137.2 67629 68792 R Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis; Gly1p complement(67629..68792) Saccharomyces cerevisiae 856665 NP_010870.1 CDS IES6 NC_001137.2 69757 70257 D Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; Ies6p 69757..70257 Saccharomyces cerevisiae 856667 NP_010871.1 CDS YEL043W NC_001137.2 70478 73348 D Yel043wp; Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold 70478..73348 Saccharomyces cerevisiae 856668 NP_010872.1 CDS GDA1 NC_001137.2 73771 75327 D Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate; Gda1p 73771..75327 Saccharomyces cerevisiae 856669 NP_010873.1 CDS YEF1 NC_001137.2 75944 77431 D Yef1p; ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes 75944..77431 Saccharomyces cerevisiae 856670 NP_010874.1 CDS UTR2 NC_001137.2 78053 79456 D Utr2p; Cell wall protein that functions in the transfer of chitin to beta(1-6)glucan; putative chitin transglycosidase; glycosylphosphatidylinositol (GPI)-anchored protein localized to the bud neck; has a role in cell wall maintenance 78053..79456 Saccharomyces cerevisiae 856671 NP_010875.1 CDS CYC7 NC_001137.2 79636 79977 R Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; Cyc7p complement(79636..79977) Saccharomyces cerevisiae 856672 NP_010876.2 CDS UTR4 NC_001137.2 80462 81145 D Utr4p; Protein of unknown function with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, found in both the cytoplasm and nucleus 80462..81145 Saccharomyces cerevisiae 856673 NP_010877.1 CDS RAD23 NC_001137.2 81407 82603 R Rad23p; Protein with ubiquitin-like N terminus, recognizes and binds damaged DNA (with Rad4p) during nucleotide excision repair; regulates Rad4p levels, subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human HR23A and HR23B proteins complement(81407..82603) Saccharomyces cerevisiae 856674 NP_010878.1 CDS ANP1 NC_001137.2 83050 84552 R Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol; Anp1p complement(83050..84552) Saccharomyces cerevisiae 856675 NP_010879.1 CDS UTR5 NC_001137.2 85045 85545 R Utr5p; Protein of unknown function; transcription may be regulated by Gcr1p complement(85045..85545) Saccharomyces cerevisiae 856676 NP_010880.1 CDS HYP2 NC_001137.2 85676 86149 D Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Anb1p; possible role in translation elongation; undergoes an essential hypusination modification; Hyp2p 85676..86149 Saccharomyces cerevisiae 856677 NP_010882.1 CDS MCM3 NC_001137.2 86937 89852 D Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; Mcm3p 86937..89852 Saccharomyces cerevisiae 856680 NP_010883.1 CDS SPF1 NC_001137.2 90258 93905 D Spf1p; P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 90258..93905 Saccharomyces cerevisiae 856681 NP_010884.1 CDS ECM10 NC_001137.2 94644 96578 D Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; Ecm10p 94644..96578 Saccharomyces cerevisiae 856682 NP_010885.1 CDS BUD16 NC_001137.2 96858 97796 R Putative pyridoxal kinase, key enzyme in vitamin B6 metabolism; involved in maintaining levels of pyridoxal 5'-phosphate, the active form of vitamin B6; required for genome integrity; homolog of E. coli PdxK; involved in bud-site selection; Bud16p complement(96858..97796) Saccharomyces cerevisiae 856683 NP_010887.1 CDS CUP5 NC_001137.2 100769 101251 D Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis; Cup5p 100769..101251 Saccharomyces cerevisiae 856686 NP_010888.1 CDS SNU13 NC_001137.2 101943 102323 D Snu13p; RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein 101943..102323 Saccharomyces cerevisiae 856687 NP_010889.1 CDS YEL025C NC_001137.2 102581 106147 R Yel025cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus complement(102581..106147) Saccharomyces cerevisiae 856688 NP_010890.1 CDS RIP1 NC_001137.2 107260 107907 D Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; Rip1p 107260..107907 Saccharomyces cerevisiae 856689 NP_010891.1 CDS YEL023C NC_001137.2 108504 110552 R Yel023cp; Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene complement(108504..110552) Saccharomyces cerevisiae 856690 NP_010892.1 CDS GEA2 NC_001137.2 111421 115800 D Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p; Gea2p 111421..115800 Saccharomyces cerevisiae 856691 NP_010893.1 CDS URA3 NC_001137.2 116167 116970 D Ura3p; Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound 116167..116970 Saccharomyces cerevisiae 856692 NP_010894.1 CDS TIM9 NC_001137.2 117211 117474 D Tim9p; Mitochondrial intermembrane space protein, forms a complex with Mrs11p/Tim10p that mediates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner 117211..117474 Saccharomyces cerevisiae 856693 NP_010895.1 CDS YEL020C NC_001137.2 118617 120299 R hypothetical protein with low sequence identity to Pdc1p; Yel020cp complement(118617..120299) Saccharomyces cerevisiae 856694 NP_010896.1 CDS MMS21 NC_001137.2 120498 121301 R SUMO ligase involved in chromosomal organization and DNA repair; essential subunit of the Mms21-Smc5-Smc6 complex; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination; Mms21p complement(120498..121301) Saccharomyces cerevisiae 856695 NP_010897.1 CDS EAF5 NC_001137.2 121471 122310 D Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex; Eaf5p 121471..122310 Saccharomyces cerevisiae 856696 NP_010898.1 CDS PMP2 NC_001137.2 122798 122929 R Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1; Pmp2p complement(122798..122929) Saccharomyces cerevisiae 856697 NP_010899.1 CDS GTT3 NC_001137.2 123657 124670 D Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery; Gtt3p 123657..124670 Saccharomyces cerevisiae 856698 NP_010900.1 CDS NPP2 NC_001137.2 124737 126218 R Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; Npp2p complement(124737..126218) Saccharomyces cerevisiae 856699 NP_010901.1 CDS EDC3 NC_001137.2 126629 128284 D Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; Edc3p 126629..128284 Saccharomyces cerevisiae 856700 NP_010903.1 CDS VAC8 NC_001137.2 128825 130561 D Vac8p; Phosphorylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions 128825..130561 Saccharomyces cerevisiae 856702 NP_010904.2 CDS UBC8 NC_001137.2 131772 132551 D Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro; Ubc8p join(131772..131776,131900..132551) Saccharomyces cerevisiae 856704 NP_010905.1 CDS GLC3 NC_001137.2 133120 135234 D Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Glc3p 133120..135234 Saccharomyces cerevisiae 856705 NP_010907.1 CDS GCN4 NC_001137.2 138918 139763 R Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels; Gcn4p complement(138918..139763) Saccharomyces cerevisiae 856709 NP_010909.1 CDS YEL007W NC_001137.2 141891 143891 D Yel007wp; Putative protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) 141891..143891 Saccharomyces cerevisiae 856711 NP_010910.1 CDS YEA6 NC_001137.2 144326 145333 D Yea6p; Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog 144326..145333 Saccharomyces cerevisiae 856712 NP_010911.1 CDS VAB2 NC_001137.2 145906 146754 R Vab2p; Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(145906..146754) Saccharomyces cerevisiae 856713 NP_010912.1 CDS YEA4 NC_001137.2 146950 147978 D Yea4p; Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER 146950..147978 Saccharomyces cerevisiae 856714 NP_010913.2 CDS GIM4 NC_001137.2 148175 148598 D Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it; Gim4p join(148175..148193,148282..148598) Saccharomyces cerevisiae 856715 NP_010914.1 CDS WBP1 NC_001137.2 148721 150013 R Wbp1p; Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum complement(148721..150013) Saccharomyces cerevisiae 856716 NP_010915.1 CDS IRC22 NC_001137.2 150300 150977 R Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52 foci; Irc22p complement(150300..150977) Saccharomyces cerevisiae 856717 NP_010916.1 CDS MNN1 NC_001137.2 153519 155807 D Mnn1p; Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family 153519..155807 Saccharomyces cerevisiae 856718 NP_010917.1 CDS NOP16 NC_001137.2 156802 157497 D Nop16p; Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis 156802..157497 Saccharomyces cerevisiae 856719 NP_010918.1 CDS PMI40 NC_001137.2 157735 159117 R Pmi40p; Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation complement(join(157735..158993,159087..159117)) Saccharomyces cerevisiae 856720 NP_010919.1 CDS FMP52 NC_001137.2 159579 160274 D Protein of unknown function, localized to the mitochondrial outer membrane; Fmp52p 159579..160274 Saccharomyces cerevisiae 856721 NP_010920.1 CDS YND1 NC_001137.2 160549 162441 D Ynd1p; Apyrase with wide substrate specificity, involved in preventing the inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates which are inhibitors of glycotransferases; partially redundant with Gda1p 160549..162441 Saccharomyces cerevisiae 856722 NP_010921.1 CDS NUG1 NC_001137.2 162722 164284 D Nug1p; GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus 162722..164284 Saccharomyces cerevisiae 856723 NP_010922.1 CDS PAC2 NC_001137.2 164526 166082 D Pac2p; Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl 164526..166082 Saccharomyces cerevisiae 856724 NP_010923.1 CDS TMA20 NC_001137.2 166236 166884 R Tma20p; Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1 complement(join(166236..166770,166874..166884)) Saccharomyces cerevisiae 856725 NP_010924.1 CDS SEC3 NC_001137.2 167807 171817 R Sec3p; Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion complement(167807..171817) Saccharomyces cerevisiae 856726 NP_010925.1 CDS NTF2 NC_001137.2 172114 172491 D Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport; Ntf2p 172114..172491 Saccharomyces cerevisiae 856727 NP_010926.1 CDS YER010C NC_001137.2 172633 173337 R Yer010cp; Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction complement(172633..173337) Saccharomyces cerevisiae 856728 NP_010927.1 CDS TIR1 NC_001137.2 175247 176011 D Tir1p; Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking 175247..176011 Saccharomyces cerevisiae 856729 NP_010928.1 CDS PRE1 NC_001137.2 177834 178430 D Pre1p; 20S proteasome beta-type subunit; localizes to the nucleus throughout the cell cycle 177834..178430 Saccharomyces cerevisiae 856731 NP_010929.1 CDS PRP22 NC_001137.2 178840 182277 D Prp22p; DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes 178840..182277 Saccharomyces cerevisiae 856732 NP_010930.1 CDS HEM14 NC_001137.2 182599 184218 D Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; Hem14p 182599..184218 Saccharomyces cerevisiae 856733 NP_076437.1 CDS BUD25 NC_001137.2 183730 184699 R Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud25p complement(join(183730..184168,184677..184699)) Saccharomyces cerevisiae 856735 NP_010931.1 CDS FAA2 NC_001137.2 184540 186774 D Long chain fatty acyl-CoA synthetase; accepts a wider range of acyl chain lengths than Faa1p, preferring C9:0-C13:0; involved in the activation of endogenous pools of fatty acids; Faa2p 184540..186774 Saccharomyces cerevisiae 856734 NP_010932.1 CDS BIM1 NC_001137.2 188276 189310 D Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; Bim1p 188276..189310 Saccharomyces cerevisiae 856736 NP_010933.1 CDS AFG3 NC_001137.2 189502 191787 R Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; Afg3p complement(189502..191787) Saccharomyces cerevisiae 856737 NP_010934.1 CDS SPC25 NC_001137.2 191958 192623 R Spc25p; Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering complement(191958..192623) Saccharomyces cerevisiae 856738 NP_010935.1 CDS ISC1 NC_001137.2 192796 194229 D Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance; Isc1p 192796..194229 Saccharomyces cerevisiae 856739 NP_010936.1 CDS SBH2 NC_001137.2 194272 194538 R Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p; Sbh2p complement(194272..194538) Saccharomyces cerevisiae 856740 NP_010937.1 CDS GPA2 NC_001137.2 195167 196516 D Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; Gpa2p 195167..196516 Saccharomyces cerevisiae 856741 NP_010938.1 CDS RPN3 NC_001137.2 196947 198518 D Rpn3p; Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control 196947..198518 Saccharomyces cerevisiae 856742 NP_010939.1 CDS SRB4 NC_001137.2 198811 200874 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Srb4p 198811..200874 Saccharomyces cerevisiae 856743 NP_010940.1 CDS PRO3 NC_001137.2 201075 201935 D Pro3p; Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis 201075..201935 Saccharomyces cerevisiae 856744 NP_010941.1 CDS YAT2 NC_001137.2 202191 204962 D Yat2p; Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane 202191..204962 Saccharomyces cerevisiae 856745 NP_010942.1 CDS GCD11 NC_001137.2 205250 206833 D Gcd11p; Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met 205250..206833 Saccharomyces cerevisiae 856746 NP_010943.1 CDS CHO1 NC_001137.2 207643 208473 R Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline; Cho1p complement(207643..208473) Saccharomyces cerevisiae 856748 NP_010944.1 CDS GAL83 NC_001137.2 208978 210231 R One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain; Gal83p complement(208978..210231) Saccharomyces cerevisiae 856749 NP_010945.1 CDS MIG3 NC_001137.2 210691 211875 R Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; Mig3p complement(210691..211875) Saccharomyces cerevisiae 856750 NP_010946.1 CDS SMB1 NC_001137.2 212586 213176 R Smb1p; Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B' complement(212586..213176) Saccharomyces cerevisiae 856751 NP_010947.2 CDS CHZ1 NC_001137.2 213436 213897 D Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; Chz1p 213436..213897 Saccharomyces cerevisiae 856752 NP_010948.1 CDS YPT31 NC_001137.2 214075 214746 R Ypt31p; GTPase of the Ypt/Rab family, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi complement(214075..214746) Saccharomyces cerevisiae 856753 NP_010949.2 CDS FIR1 NC_001137.2 215062 217692 D Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate; Fir1p 215062..217692 Saccharomyces cerevisiae 856754 NP_010950.1 CDS ZRG8 NC_001137.2 218056 221286 R Zrg8p; Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency complement(218056..221286) Saccharomyces cerevisiae 856755 NP_010951.1 CDS YER034W NC_001137.2 221845 222402 D Yer034wp; Putative protein of unknown function; non-essential gene; expression induced upon calcium shortage 221845..222402 Saccharomyces cerevisiae 856756 NP_010952.1 CDS EDC2 NC_001137.2 222638 223075 D RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; Edc2p 222638..223075 Saccharomyces cerevisiae 856757 NP_010953.1 CDS ARB1 NC_001137.2 223366 225198 R Arb1p; ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p complement(223366..225198) Saccharomyces cerevisiae 856758 NP_010954.1 CDS PHM8 NC_001137.2 225888 226853 D Protein of unknown function, expression is induced by low phosphate levels and by inactivation of Pho85p; Phm8p 225888..226853 Saccharomyces cerevisiae 856759 NP_010955.1 CDS KRE29 NC_001137.2 226857 228251 R Kre29p; Essential subunit of the Mms21-Smc5-Smc6 complex; protein of unknown function; required for growth and DNA repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance complement(226857..228251) Saccharomyces cerevisiae 856760 NP_010956.1 CDS HVG1 NC_001137.2 228455 229204 R Protein of unknown function, has homology to Vrg4p; Hvg1p complement(228455..229204) Saccharomyces cerevisiae 856761 NP_010957.1 CDS YER039C-A NC_001137.2 229262 229480 R Yer039c-ap; Putative protein of unknown function; YER039C-A is not an essential gene complement(229262..229480) Saccharomyces cerevisiae 856762 NP_010958.1 CDS GLN3 NC_001137.2 229794 231986 D Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source; Gln3p 229794..231986 Saccharomyces cerevisiae 856763 NP_010959.1 CDS YEN1 NC_001137.2 232460 234739 D Yen1p; Protein of unknown function, has similarity to endonuclease Rth1p; potentially phosphorylated by Cdc28p 232460..234739 Saccharomyces cerevisiae 856764 NP_010960.1 CDS MXR1 NC_001137.2 234936 235490 D Peptide methionine sulfoxide reductase, reverses the oxidation of methionine residues; involved in oxidative damage repair, providing resistance to oxidative stress and regulation of lifespan; Mxr1p 234936..235490 Saccharomyces cerevisiae 856765 NP_010961.1 CDS SAH1 NC_001137.2 235769 237118 R Sah1p; S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor complement(235769..237118) Saccharomyces cerevisiae 856766 NP_010962.1 CDS ERG28 NC_001137.2 237569 238015 R Erg28p; Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p complement(237569..238015) Saccharomyces cerevisiae 856767 NP_010963.1 CDS MEI4 NC_001137.2 238459 239773 R Meiosis-specific protein involved in recombination; required for chromosome synapsis; required for production of viable spores; Mei4p complement(join(238459..239622,239711..239773)) Saccharomyces cerevisiae 856768 NP_010964.1 CDS ACA1 NC_001137.2 240031 241500 R Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, may regulate transcription of genes involved in utilization of non-optimal carbon sources; Aca1p complement(240031..241500) Saccharomyces cerevisiae 856769 NP_010965.1 CDS SPO73 NC_001137.2 243179 243610 D Spo73p; Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis 243179..243610 Saccharomyces cerevisiae 856770 NP_010966.1 CDS SAP1 NC_001137.2 243809 246502 R Sap1p; Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system complement(243809..246502) Saccharomyces cerevisiae 856771 NP_010967.1 CDS CAJ1 NC_001137.2 246981 248156 R Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly; Caj1p complement(246981..248156) Saccharomyces cerevisiae 856772 NP_010968.1 CDS ISD11 NC_001137.2 250717 251001 D Protein required for mitochondrial iron-sulfur cluster biosynthesis; Isd11p 250717..251001 Saccharomyces cerevisiae 856774 NP_010969.1 CDS TPA1 NC_001137.2 251727 253661 D Tpa1p; Protein of unknown function; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; has a role in translation termination efficiency, mRNA poly(A) tail length and mRNA stability 251727..253661 Saccharomyces cerevisiae 856775 NP_010970.2 CDS RSM18 NC_001137.2 253970 254386 R Rsm18p; Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein complement(253970..254386) Saccharomyces cerevisiae 856776 NP_010971.1 CDS JHD1 NC_001137.2 254655 256133 D JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe; Jhd1p 254655..256133 Saccharomyces cerevisiae 856777 NP_010972.1 CDS HOM3 NC_001137.2 256374 257957 R Hom3p; Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis complement(256374..257957) Saccharomyces cerevisiae 856778 NP_010973.1 CDS PIC2 NC_001137.2 258736 259638 R Pic2p; Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature complement(258736..259638) Saccharomyces cerevisiae 856779 NP_061492.1 CDS YER053C-A NC_001137.2 260932 261045 R Yer053c-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum complement(260932..261045) Saccharomyces cerevisiae 856780 NP_010974.1 CDS GIP2 NC_001137.2 262051 263697 R Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; Gip2p complement(262051..263697) Saccharomyces cerevisiae 856781 NP_010975.1 CDS HIS1 NC_001137.2 264891 265784 R His1p; ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control complement(264891..265784) Saccharomyces cerevisiae 856782 NP_010976.1 CDS FCY2 NC_001137.2 266511 268112 R Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; Fcy2p complement(266511..268112) Saccharomyces cerevisiae 856783 NP_010977.2 CDS RPL34A NC_001137.2 269421 270183 R Rpl34ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein complement(join(269421..269749,270147..270183)) Saccharomyces cerevisiae 856784 NP_010978.1 CDS HMF1 NC_001137.2 270735 271124 R Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; Hmf1p complement(270735..271124) Saccharomyces cerevisiae 856785 NP_010979.1 CDS PET117 NC_001137.2 271766 272089 D Protein required for assembly of cytochrome c oxidase; Pet117p 271766..272089 Saccharomyces cerevisiae 856786 NP_010980.1 CDS PCL6 NC_001137.2 272622 273884 D Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; Pcl6p 272622..273884 Saccharomyces cerevisiae 856787 NP_010981.1 CDS FCY21 NC_001137.2 274565 276151 D Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function; Fcy21p 274565..276151 Saccharomyces cerevisiae 856788 NP_010982.1 CDS FCY22 NC_001137.2 276570 278162 D Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function; Fcy22p 276570..278162 Saccharomyces cerevisiae 856789 NP_010983.1 CDS CEM1 NC_001137.2 278296 279624 R Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration; Cem1p complement(278296..279624) Saccharomyces cerevisiae 856790 NP_010984.1 CDS HOR2 NC_001137.2 279928 280680 R One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition; Hor2p complement(279928..280680) Saccharomyces cerevisiae 856791 NP_010985.1 CDS THO1 NC_001137.2 281708 282364 D Tho1p; Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation 281708..282364 Saccharomyces cerevisiae 856792 NP_010986.1 CDS YER064C NC_001137.2 282703 284220 R Yer064cp; Non-essential nuclear protein; null mutation has global effects on transcription complement(282703..284220) Saccharomyces cerevisiae 856793 NP_010987.1 CDS ICL1 NC_001137.2 285239 286912 R Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose; Icl1p complement(285239..286912) Saccharomyces cerevisiae 856794 NP_010988.1 CDS YER066W NC_001137.2 290240 290797 D Yer066wp; Putative protein of unknown function; YER066W is not an essential gene 290240..290797 Saccharomyces cerevisiae 856796 NP_010990.1 CDS YER067W NC_001137.2 292064 292549 D Yer067wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene 292064..292549 Saccharomyces cerevisiae 856797 NP_010991.1 CDS MOT2 NC_001137.2 293048 294811 D Mot2p; Component of the CCR4-NOT complex, which has multiple roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; with Ubc4p, ubiquitylates subunits of the nascent polypeptide-associated complex (NAC) 293048..294811 Saccharomyces cerevisiae 856799 NP_010992.1 CDS ARG5,6 NC_001137.2 295408 297999 D Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p; Arg5,6p 295408..297999 Saccharomyces cerevisiae 856800 NP_010993.1 CDS RNR1 NC_001137.2 298948 301614 D Rnr1p; Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits 298948..301614 Saccharomyces cerevisiae 856801 NP_010994.1 CDS YER071C NC_001137.2 301945 302325 R Yer071cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(301945..302325) Saccharomyces cerevisiae 856802 NP_010995.1 CDS VTC1 NC_001137.2 302804 303193 D Vtc1p; Vacuolar transporter chaperon (VTC) involved in distributing V-ATPase and other membrane proteins; together with other VTC proteins, forms a heterotetrameric complex that associates with the SNARE Nyv1p and the V0 sector of the V-ATPase 302804..303193 Saccharomyces cerevisiae 856803 NP_010996.1 CDS ALD5 NC_001137.2 304027 305589 D Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed; Ald5p 304027..305589 Saccharomyces cerevisiae 856804 NP_010997.1 CDS RPS24A NC_001137.2 306319 307192 D Rps24ap; Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein join(306319..306321,306788..307192) Saccharomyces cerevisiae 856805 NP_219496.2 CDS YOS1 NC_001137.2 307649 308119 D Yos1p; Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p join(307649..307742,307845..307952,308064..308119) Saccharomyces cerevisiae 856806 NP_010998.1 CDS PTP3 NC_001137.2 308409 311195 R Ptp3p; Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm complement(308409..311195) Saccharomyces cerevisiae 856807 NP_010999.1 CDS YER076C NC_001137.2 312586 313494 R Yer076cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization complement(312586..313494) Saccharomyces cerevisiae 856809 NP_011000.1 CDS YER077C NC_001137.2 314530 316596 R Yer077cp complement(314530..316596) Saccharomyces cerevisiae 856810 NP_011001.1 CDS YER078C NC_001137.2 316803 318338 R Yer078cp; Metallopeptidase, localized to the mitochondrial matrix complement(316803..318338) Saccharomyces cerevisiae 856811 NP_878068.1 CDS YER078W-A NC_001137.2 318642 318806 D Yer078w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 318642..318806 Saccharomyces cerevisiae 1466536 NP_011002.1 CDS YER079W NC_001137.2 318916 319548 D Yer079wp; Putative protein of unknown function 318916..319548 Saccharomyces cerevisiae 856812 NP_011003.1 CDS AIM9 NC_001137.2 319959 321842 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss (petite formation); Aim9p 319959..321842 Saccharomyces cerevisiae 856813 NP_011004.1 CDS SER3 NC_001137.2 322682 324091 D Ser3p; 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p 322682..324091 Saccharomyces cerevisiae 856814 NP_011005.1 CDS UTP7 NC_001137.2 324268 325932 R Utp7p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(324268..325932) Saccharomyces cerevisiae 856815 NP_011006.2 CDS GET2 NC_001137.2 326170 327027 R Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function; Get2p complement(326170..327027) Saccharomyces cerevisiae 856817 NP_011008.1 CDS YER085C NC_001137.2 327615 328136 R Yer085cp; Putative protein of unknown function complement(327615..328136) Saccharomyces cerevisiae 856818 NP_011009.1 CDS ILV1 NC_001137.2 328473 330203 D Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation; Ilv1p 328473..330203 Saccharomyces cerevisiae 856819 NP_011010.1 CDS AIM10 NC_001137.2 330572 332302 D Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant displays decreased frequency of mitochondrial genome loss (petite formation), severe growth defect in minimal glycerol media; Aim10p 330572..332302 Saccharomyces cerevisiae 856820 NP_011011.1 CDS SBH1 NC_001137.2 332578 332826 R Sbh1p; Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p complement(332578..332826) Saccharomyces cerevisiae 856821 NP_011012.1 CDS DOT6 NC_001137.2 333172 335184 R Protein of unknown function, involved in telomeric gene silencing and filamentation; Dot6p complement(333172..335184) Saccharomyces cerevisiae 856822 NP_011013.1 CDS PTC2 NC_001137.2 335942 337336 R Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation; Ptc2p complement(335942..337336) Saccharomyces cerevisiae 856823 NP_011014.1 CDS TRP2 NC_001137.2 337945 339468 D Trp2p; Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p 337945..339468 Saccharomyces cerevisiae 856824 NP_011015.1 CDS MET6 NC_001137.2 339860 342163 R Met6p; Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs complement(339860..342163) Saccharomyces cerevisiae 856825 NP_011017.1 CDS IES5 NC_001137.2 342851 343228 D Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; Ies5p 342851..343228 Saccharomyces cerevisiae 856827 NP_011018.1 CDS TSC11 NC_001137.2 343316 347608 R Tsc11p; Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain complement(343316..347608) Saccharomyces cerevisiae 856828 NP_011019.1 CDS AIM11 NC_001137.2 347908 348396 R Putative protein of unknown function; YER093C-A contains an intron; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim11p complement(join(347908..348197,348273..348396)) Saccharomyces cerevisiae 856829 NP_011020.1 CDS PUP3 NC_001137.2 348725 349342 R Pup3p; Beta subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 complement(348725..349342) Saccharomyces cerevisiae 856830 NP_011021.1 CDS RAD51 NC_001137.2 349976 351178 D Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein; Rad51p 349976..351178 Saccharomyces cerevisiae 856831 NP_011022.1 CDS SHC1 NC_001137.2 351694 353232 D Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH; Shc1p 351694..353232 Saccharomyces cerevisiae 856832 NP_011024.1 CDS UBP9 NC_001137.2 355462 357726 D Ubp9p; Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions 355462..357726 Saccharomyces cerevisiae 856835 NP_011025.1 CDS PRS2 NC_001137.2 358101 359057 R Prs2p; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes complement(358101..359057) Saccharomyces cerevisiae 856836 NP_011026.1 CDS UBC6 NC_001137.2 359558 360310 D Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; Ubc6p 359558..360310 Saccharomyces cerevisiae 856837 NP_011027.1 CDS AST2 NC_001137.2 360498 361790 R Protein that may have a role in targeting of plasma membrane [H+]ATPase (Pma1p) to the plasma membrane, as suggested by analysis of genetic interactions; Ast2p complement(360498..361790) Saccharomyces cerevisiae 856838 NP_011028.1 CDS RPS8B NC_001137.2 363096 363698 D Rps8bp; Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein 363096..363698 Saccharomyces cerevisiae 856839 NP_011029.1 CDS SSA4 NC_001137.2 364585 366513 D Ssa4p; Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation 364585..366513 Saccharomyces cerevisiae 856840 NP_011030.1 CDS RTT105 NC_001137.2 366798 367424 D Rtt105p; Protein with a role in regulation of Ty1 transposition 366798..367424 Saccharomyces cerevisiae 856841 NP_011031.1 CDS NUP157 NC_001137.2 367834 372009 R Nup157p; Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p complement(367834..372009) Saccharomyces cerevisiae 856842 NP_011032.1 CDS MAM1 NC_001137.2 372322 373230 D Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle; Mam1p 372322..373230 Saccharomyces cerevisiae 856843 NP_011033.1 CDS GLE2 NC_001137.2 373444 374541 R Gle2p; Component of the nuclear pore complex required for polyadenylated RNA export but not for protein import, homologous to S. pombe Rae1p complement(373444..374541) Saccharomyces cerevisiae 856844 NP_011034.1 CDS FLO8 NC_001137.2 375211 377610 R Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene; Flo8p complement(375211..377610) Saccharomyces cerevisiae 856845 NP_011035.1 CDS KAP123 NC_001137.2 378758 382099 R Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1; Kap123p complement(378758..382099) Saccharomyces cerevisiae 856846 NP_011036.1 CDS SWI4 NC_001137.2 382591 385872 R Swi4p; DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair complement(382591..385872) Saccharomyces cerevisiae 856847 NP_011037.1 CDS LSM4 NC_001137.2 387228 387791 D Lsm4p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA 387228..387791 Saccharomyces cerevisiae 856848 NP_011038.1 CDS TMN3 NC_001137.2 387928 390048 R Tmn3p; Non-essential golgi localized protein of unknown function; contains niine potential transmembrane domains and has similarity to the endosomal protein, Emp70p complement(387928..390048) Saccharomyces cerevisiae 856849 NP_011039.1 CDS BOI2 NC_001137.2 390586 393708 R Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; Boi2p complement(390586..393708) Saccharomyces cerevisiae 856850 NP_011040.1 CDS SPR6 NC_001137.2 394288 394863 R Spr6p; Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation complement(394288..394863) Saccharomyces cerevisiae 856851 NP_011041.1 CDS SLX8 NC_001137.2 395344 396168 R Subunit of the Slx5-Slx8 substrate-specific ubiquitin ligase complex; stimulated by prior attachment of SUMO to the substrate; Slx8p complement(395344..396168) Saccharomyces cerevisiae 856852 NP_011042.1 CDS RPL23B NC_001137.2 396765 397649 D Rpl23bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli L14 and rat L23 ribosomal proteins join(396765..396806,397278..397649) Saccharomyces cerevisiae 856853 NP_011043.1 CDS SHO1 NC_001137.2 397948 399051 R Transmembrane osmosensor, participates in activation of both the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; Sho1p complement(397948..399051) Saccharomyces cerevisiae 856854 NP_011044.1 CDS AVT6 NC_001137.2 399492 400838 R Vacuolar amino acid transporter, exports aspartate and glutamate from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt6p complement(399492..400838) Saccharomyces cerevisiae 856855 NP_011046.1 CDS SCS2 NC_001137.2 401131 401865 D Scs2p; Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog 401131..401865 Saccharomyces cerevisiae 856856 NP_011047.1 CDS YER121W NC_001137.2 402371 402715 D Yer121wp; Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy 402371..402715 Saccharomyces cerevisiae 856858 NP_011048.1 CDS GLO3 NC_001137.2 402867 404348 R Glo3p; ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p complement(402867..404348) Saccharomyces cerevisiae 856859 NP_011049.2 CDS YCK3 NC_001137.2 404809 406383 D Yck3p; Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of the HOPS complex subunit, Vps41p; shares overlapping essential functions with Hrr25p 404809..406383 Saccharomyces cerevisiae 856860 NP_011050.1 CDS DSE1 NC_001137.2 407338 409059 R Dse1p; Daughter cell-specific protein, may participate in pathways regulating cell wall metabolism; deletion affects cell separation after division and sensitivity to drugs targeted against the cell wall complement(407338..409059) Saccharomyces cerevisiae 856861 NP_011051.1 CDS RSP5 NC_001137.2 410185 412614 D Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation; functions in multivesicular body sorting, heat shock response and ubiquitylation of arrested RNAPII; contains a hect (homologous to E6-AP carboxyl terminus) domain; Rsp5p 410185..412614 Saccharomyces cerevisiae 856862 NP_011052.1 CDS NSA2 NC_001137.2 413390 414175 R Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA; Nsa2p complement(413390..414175) Saccharomyces cerevisiae 856863 NP_011053.1 CDS LCP5 NC_001137.2 414477 415550 D Lcp5p; Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus 414477..415550 Saccharomyces cerevisiae 856864 NP_011054.1 CDS YER128W NC_001137.2 415855 416466 D Yer128wp; Putative protein of unknown function 415855..416466 Saccharomyces cerevisiae 856865 NP_011055.1 CDS SAK1 NC_001137.2 417277 420705 D Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; Sak1p 417277..420705 Saccharomyces cerevisiae 856866 NP_011056.1 CDS YER130C NC_001137.2 421111 422442 R Yer130cp complement(421111..422442) Saccharomyces cerevisiae 856867 NP_011057.1 CDS RPS26B NC_001137.2 423948 424307 D Rps26bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein 423948..424307 Saccharomyces cerevisiae 856868 NP_011058.1 CDS PMD1 NC_001137.2 425184 430445 R Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; Pmd1p complement(425184..430445) Saccharomyces cerevisiae 856869 NP_011059.1 CDS GLC7 NC_001137.2 432491 433954 D Glc7p; Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p join(432491..432667,433193..433954) Saccharomyces cerevisiae 856870 NP_011060.1 CDS YER134C NC_001137.2 437263 437799 R Yer134cp; Putative protein of unknown function; non-essential gene complement(437263..437799) Saccharomyces cerevisiae 856873 NP_011062.1 CDS GDI1 NC_001137.2 439612 440967 D Gdi1p; GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins 439612..440967 Saccharomyces cerevisiae 856876 NP_011063.1 CDS YER137C NC_001137.2 441369 441815 R Yer137cp; Putative protein of unknown function complement(441369..441815) Saccharomyces cerevisiae 856877 NP_011064.1 CDS YER138C NC_001137.2 443752 449020 R Yer138cp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(443752..447717,447719..449020)) Saccharomyces cerevisiae 856879 NP_058155.1 CDS YER137C-A NC_001137.2 447698 449020 R Yer137c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(447698..449020) Saccharomyces cerevisiae 856880 NP_011065.1 CDS YER138W-A NC_001137.2 449470 449574 D Yer138w-ap; Putative protein of unknown function 449470..449574 Saccharomyces cerevisiae 856881 NP_011066.1 CDS RTR1 NC_001137.2 450558 451238 R Rtr1p; Putative protein of unknown function; YER139C has been shown to be required for growth at high temperatures; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(450558..451238) Saccharomyces cerevisiae 856882 NP_011067.1 CDS YER140W NC_001137.2 451560 453230 D Yer140wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 451560..453230 Saccharomyces cerevisiae 856883 NP_011068.1 CDS COX15 NC_001137.2 453454 454914 D Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase; Cox15p 453454..454914 Saccharomyces cerevisiae 856884 NP_011069.1 CDS MAG1 NC_001137.2 455141 456031 R Mag1p; 3-methyl-adenine DNA glycosylase involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired complement(455141..456031) Saccharomyces cerevisiae 856885 NP_011070.1 CDS DDI1 NC_001137.2 456314 457600 D Ddi1p; DNA damage-inducible v-SNARE binding protein, contains a ubiquitin-associated (UBA) domain, may act as a negative regulator of constitutive exocytosis, may play a role in S-phase checkpoint control 456314..457600 Saccharomyces cerevisiae 856886 NP_011071.1 CDS UBP5 NC_001137.2 457801 460218 R Ubp5p; Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck complement(457801..460218) Saccharomyces cerevisiae 856887 NP_011072.1 CDS FTR1 NC_001137.2 460521 461735 R High affinity iron permease involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; Ftr1p complement(460521..461735) Saccharomyces cerevisiae 856888 NP_011073.1 CDS LSM5 NC_001137.2 462580 462861 D Lsm5p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA 462580..462861 Saccharomyces cerevisiae 856889 NP_011074.1 CDS SCC4 NC_001137.2 462963 464837 R Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX; Scc4p complement(462963..464837) Saccharomyces cerevisiae 856890 NP_011075.1 CDS SPT15 NC_001137.2 465298 466020 D TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability; Spt15p 465298..466020 Saccharomyces cerevisiae 856891 NP_011076.1 CDS PEA2 NC_001137.2 466203 467465 R Pea2p; Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth complement(466203..467465) Saccharomyces cerevisiae 856892 NP_011077.1 CDS SPI1 NC_001137.2 468365 468811 D Spi1p; GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p 468365..468811 Saccharomyces cerevisiae 856893 NP_011078.1 CDS UBP3 NC_001137.2 469681 472419 R Ubp3p; Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi compartments; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin complement(469681..472419) Saccharomyces cerevisiae 856895 NP_011079.1 CDS YER152C NC_001137.2 472652 473983 R Yer152cp; Putative protein of unknown function; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene complement(472652..473983) Saccharomyces cerevisiae 856896 NP_011080.1 CDS PET122 NC_001137.2 474036 474800 R Pet122p; Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane complement(474036..474800) Saccharomyces cerevisiae 856897 NP_011081.1 CDS OXA1 NC_001137.2 475015 476223 D Oxa1p; Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals 475015..476223 Saccharomyces cerevisiae 856898 NP_011082.1 CDS BEM2 NC_001137.2 476340 482843 R Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; Bem2p complement(476340..482843) Saccharomyces cerevisiae 856899 NP_011083.1 CDS YER156C NC_001137.2 483320 484336 R Yer156cp; Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(483320..484336) Saccharomyces cerevisiae 856900 NP_011084.1 CDS COG3 NC_001137.2 484783 487188 D Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog3p 484783..487188 Saccharomyces cerevisiae 856901 NP_011085.1 CDS YER158C NC_001137.2 488852 490573 R Yer158cp; Protein of unknown function, has similarity to Afr1p; potentially phosphorylated by Cdc28p complement(488852..490573) Saccharomyces cerevisiae 856903 NP_011086.1 CDS BUR6 NC_001137.2 491525 491953 R Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; Bur6p complement(491525..491953) Saccharomyces cerevisiae 856904 NP_011087.1 CDS YER160C NC_001137.2 492851 498119 R Yer160cp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(492851..496816,496818..498119)) Saccharomyces cerevisiae 856906 NP_058156.1 CDS YER159C-A NC_001137.2 496797 498119 R Yer159c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(496797..498119) Saccharomyces cerevisiae 856907 NP_011088.1 CDS SPT2 NC_001137.2 499342 500343 R Spt2p; Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins complement(499342..500343) Saccharomyces cerevisiae 856908 NP_011089.1 CDS RAD4 NC_001137.2 500625 502889 R Rad4p; Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human XPC protein complement(500625..502889) Saccharomyces cerevisiae 856909 NP_011090.1 CDS YER163C NC_001137.2 503079 503777 R Yer163cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(503079..503777) Saccharomyces cerevisiae 856910 NP_011091.1 CDS CHD1 NC_001137.2 505387 509793 D Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes; Chd1p 505387..509793 Saccharomyces cerevisiae 856911 NP_011092.1 CDS PAB1 NC_001137.2 510368 512101 D Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; Pab1p 510368..512101 Saccharomyces cerevisiae 856912 NP_011093.1 CDS DNF1 NC_001137.2 512739 517454 D Dnf1p; Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase 512739..517454 Saccharomyces cerevisiae 856913 NP_011094.1 CDS BCK2 NC_001137.2 518211 520766 D Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations; Bck2p 518211..520766 Saccharomyces cerevisiae 856914 NP_011095.1 CDS CCA1 NC_001137.2 521024 522664 R Cca1p; ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites complement(521024..522664) Saccharomyces cerevisiae 856915 NP_011096.1 CDS RPH1 NC_001137.2 523364 525754 D Rph1p; JmjC domain-containing histone demethylase which can specifically demethylate H3K36 tri- and dimethyl modification states; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway 523364..525754 Saccharomyces cerevisiae 856916 NP_011097.1 CDS ADK2 NC_001137.2 525969 526646 D Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background; Adk2p 525969..526646 Saccharomyces cerevisiae 856917 NP_011098.1 CDS RAD3 NC_001137.2 527077 529413 D Rad3p; 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA polymerase II transcription initiation factor TFIIH; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein 527077..529413 Saccharomyces cerevisiae 856918 NP_011099.1 CDS BRR2 NC_001137.2 529525 536016 R RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD; Brr2p complement(529525..536016) Saccharomyces cerevisiae 856919 NP_011100.1 CDS RAD24 NC_001137.2 536295 538274 D Rad24p; Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein 536295..538274 Saccharomyces cerevisiae 856920 NP_011101.1 CDS GRX4 NC_001137.2 538429 539163 R Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; Grx4p complement(538429..539163) Saccharomyces cerevisiae 856921 NP_011102.1 CDS TMT1 NC_001137.2 539459 540358 R Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle; Tmt1p complement(539459..540358) Saccharomyces cerevisiae 856922 NP_878069.1 CDS YER175W-A NC_001137.2 540645 540809 D Yer175w-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 540645..540809 Saccharomyces cerevisiae 1466537 NP_011103.1 CDS ECM32 NC_001137.2 541685 545050 D Ecm32p; DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes 541685..545050 Saccharomyces cerevisiae 856923 NP_011104.1 CDS BMH1 NC_001137.2 545606 546409 D Bmh1p; 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling 545606..546409 Saccharomyces cerevisiae 856924 NP_011105.2 CDS PDA1 NC_001137.2 546812 548074 D Pda1p; E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose 546812..548074 Saccharomyces cerevisiae 856925 NP_011106.1 CDS DMC1 NC_001137.2 548416 549512 D Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; Dmc1p join(548416..548547,548640..549512) Saccharomyces cerevisiae 856926 NP_011107.1 CDS ISC10 NC_001137.2 549719 550522 R Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells; Isc10p complement(549719..550522) Saccharomyces cerevisiae 856927 NP_796378.1 CDS SLO1 NC_001137.2 550860 551117 R Slo1p; Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO complement(550860..551117) Saccharomyces cerevisiae 856928 NP_011109.1 CDS FMP10 NC_001137.2 552520 553254 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp10p 552520..553254 Saccharomyces cerevisiae 856931 NP_011110.1 CDS FAU1 NC_001137.2 553329 553964 R Fau1p; 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis complement(553329..553964) Saccharomyces cerevisiae 856932 NP_011111.1 CDS YER184C NC_001137.2 556291 558675 R Yer184cp; Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source complement(556291..558675) Saccharomyces cerevisiae 856933 NP_011112.1 CDS PUG1 NC_001137.2 559449 560360 D Pug1p; Plasma membrane protein involved in protoporphyrin uptake; similar in sequence to RSB1, which is involved in sphingoid long-chain base release 559449..560360 Saccharomyces cerevisiae 856934 NP_011113.1 CDS YER186C NC_001137.2 561700 562620 R Yer186cp; Putative protein of unknown function complement(561700..562620) Saccharomyces cerevisiae 856935 NP_011114.1 CDS YER187W NC_001137.2 566225 566650 D Yer187wp; Putative protein of unknown function; induced in respiratory-deficient cells 566225..566650 Saccharomyces cerevisiae 856937 NP_878070.1 CDS YER188C-A NC_001137.2 569603 569902 R Yer188c-ap; Putative protein of unknown function complement(569603..569902) Saccharomyces cerevisiae 1466538 NP_011116.1 CDS YER189W NC_001137.2 571150 571518 D Yer189wp; Putative protein of unknown function 571150..571518 Saccharomyces cerevisiae 856939 NP_011117.1 CDS YRF1-2 NC_001137.2 571475 576520 D Yrf1-2p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 571475..576520 Saccharomyces cerevisiae 856940 NP_001073292.1 CDS YER190C-B NC_001137.2 575675 576157 R Yer190c-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(575675..576157) Saccharomyces cerevisiae 4594642 NP_116587.1 CDS YFL068W NC_001138.4 53 535 D Yfl068wp; Putative protein of unknown function 53..535 Saccharomyces cerevisiae 850476 NP_116588.1 CDS YFL067W NC_001138.4 836 1363 D Yfl067wp; Protein of unknown function, down-regulated at low calcium levels 836..1363 Saccharomyces cerevisiae 850477 NP_116589.1 CDS YFL066C NC_001138.4 1437 2615 R Yfl066cp; Helicase-like protein encoded within the telomeric Y' element complement(1437..2615) Saccharomyces cerevisiae 850478 NP_116590.1 CDS YFL065C NC_001138.4 3030 3338 R Yfl065cp; Putative protein of unknown function complement(3030..3338) Saccharomyces cerevisiae 850479 NP_116591.1 CDS YFL064C NC_001138.4 3322 3846 R Yfl064cp; Putative protein of unknown function complement(3322..3846) Saccharomyces cerevisiae 850480 NP_116593.1 CDS COS4 NC_001138.4 6426 7565 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos4p 6426..7565 Saccharomyces cerevisiae 850482 NP_116594.1 CDS DDI2 NC_001138.4 9545 10222 D Protein whose expression is induced by DNA damage; Ddi2p 9545..10222 Saccharomyces cerevisiae 850483 NP_116595.1 CDS SNO3 NC_001138.4 10301 10969 R Sno3p; Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin complement(10301..10969) Saccharomyces cerevisiae 850484 NP_116596.1 CDS SNZ3 NC_001138.4 11363 12259 D Snz3p; Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3 11363..12259 Saccharomyces cerevisiae 850485 NP_116597.1 CDS THI5 NC_001138.4 12929 13951 D Thi5p; Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 12929..13951 Saccharomyces cerevisiae 850486 NP_116598.1 CDS AAD16 NC_001138.4 14305 14763 R Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; Aad16p complement(14305..14763) Saccharomyces cerevisiae 850487 NP_116599.1 CDS AAD6 NC_001138.4 14793 15431 R Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase, involved in the oxidative stress response; Aad6p complement(14793..15431) Saccharomyces cerevisiae 850488 NP_116600.1 CDS AGP3 NC_001138.4 17004 18680 D Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; Agp3p 17004..18680 Saccharomyces cerevisiae 850489 NP_116601.1 CDS YFL054C NC_001138.4 20847 22787 R Yfl054cp; Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol complement(20847..22787) Saccharomyces cerevisiae 850490 NP_116602.1 CDS DAK2 NC_001138.4 23423 25198 D Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation; Dak2p 23423..25198 Saccharomyces cerevisiae 850491 NP_116603.1 CDS YFL052W NC_001138.4 28232 29629 D Yfl052wp; Putative zinc cluster protein that contains a DNA binding domain; null mutant sensitive to calcofluor white, low osmolarity and heat, suggesting a role for YFL052Wp in cell wall integrity 28232..29629 Saccharomyces cerevisiae 850492 NP_116576.1 CDS YFL051C NC_001138.4 30058 30540 R Yfl051cp; Putative protein of unknown function; YFL051C is not an essential gene complement(30058..30540) Saccharomyces cerevisiae 850493 NP_116604.1 CDS ALR2 NC_001138.4 33272 35848 R Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; Alr2p complement(33272..35848) Saccharomyces cerevisiae 850494 NP_116605.1 CDS SWP82 NC_001138.4 36803 38674 D Swp82p; Member of the SWI/SNF chromatin remodeling complex in which it plays an as yet unidentified role; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p 36803..38674 Saccharomyces cerevisiae 850495 NP_116606.1 CDS EMP47 NC_001138.4 38843 40180 R Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport; Emp47p complement(38843..40180) Saccharomyces cerevisiae 850496 NP_428268.1 CDS RGD2 NC_001138.4 40421 42565 D Rgd2p; GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p 40421..42565 Saccharomyces cerevisiae 850497 NP_116608.1 CDS FMP32 NC_001138.4 42815 43438 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp32p 42815..43438 Saccharomyces cerevisiae 850498 NP_116609.1 CDS SEC53 NC_001138.4 43628 44392 R Sec53p; Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen complement(43628..44392) Saccharomyces cerevisiae 850499 NP_116610.1 CDS OTU1 NC_001138.4 44655 45560 R Otu1p; Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family complement(44655..45560) Saccharomyces cerevisiae 850500 NP_116611.1 CDS YFL042C NC_001138.4 45720 47744 R Yfl042cp; Putative protein of unknown function; YFL042C is not an essential gene complement(45720..47744) Saccharomyces cerevisiae 850501 NP_878071.1 CDS YFL041W-A NC_001138.4 48734 48925 D Yfl041w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 48734..48925 Saccharomyces cerevisiae 1466401 NP_116612.1 CDS FET5 NC_001138.4 49139 51007 D Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport; Fet5p 49139..51007 Saccharomyces cerevisiae 850502 NP_116613.1 CDS YFL040W NC_001138.4 51350 52972 D Yfl040wp; Putative transporter, member of the sugar porter family; YFL040W is not an essential gene 51350..52972 Saccharomyces cerevisiae 850503 NP_116614.1 CDS ACT1 NC_001138.4 53260 54695 R Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions; Act1p complement(join(53260..54377,54686..54695)) Saccharomyces cerevisiae 850504 NP_116615.1 CDS YPT1 NC_001138.4 55365 55985 R Ypt1p; Ras-like small GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) complement(55365..55985) Saccharomyces cerevisiae 850505 NP_116616.1 CDS TUB2 NC_001138.4 56335 57708 D Tub2p; Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules 56335..57708 Saccharomyces cerevisiae 850506 NP_116617.1 CDS RPO41 NC_001138.4 58781 62836 D Rpo41p; Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition 58781..62836 Saccharomyces cerevisiae 850507 NP_116618.1 CDS MOB2 NC_001138.4 63015 63992 R Mob2p; Component of the RAM signaling network, localizes and activates the Ace2p in the daughter cell nucleus to direct daughter cell-specific transcription of several genes involved in cell separation; Mob1p-like protein complement(join(63015..63858,63973..63992)) Saccharomyces cerevisiae 850508 NP_116619.1 CDS RPL22B NC_001138.4 64242 64931 R Rpl22bp; Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein complement(join(64242..64598,64920..64931)) Saccharomyces cerevisiae 850509 NP_116583.1 CDS YFL034W NC_001138.4 65475 68696 D Yfl034wp; Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk 65475..68696 Saccharomyces cerevisiae 850510 NP_116620.1 CDS RIM15 NC_001138.4 69113 74425 R Rim15p; Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase complement(69113..74425) Saccharomyces cerevisiae 850511 NP_116622.1 CDS HAC1 NC_001138.4 75177 76145 D bZIP transcription factor (ATF/CREB1 homolog) that regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway utilizing Ire1p, Trl1p and Ada5p facilitates efficient Hac1p synthesis; Hac1p join(75177..75837,76090..76145) Saccharomyces cerevisiae 850513 NP_116623.1 CDS AGX1 NC_001138.4 76829 77986 D Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases; Agx1p 76829..77986 Saccharomyces cerevisiae 850514 NP_116624.1 CDS CAK1 NC_001138.4 78053 79159 R Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; Cak1p complement(78053..79159) Saccharomyces cerevisiae 850515 NP_116625.1 CDS CAF16 NC_001138.4 79342 80211 R Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation; putative ABC ATPase; interacts with Ssn2p, Ssn3p, and Ssn8p; Caf16p complement(79342..80211) Saccharomyces cerevisiae 850516 NP_444296.1 CDS GYP8 NC_001138.4 80417 81910 R Gyp8p; GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport complement(80417..81910) Saccharomyces cerevisiae 850517 NP_116627.1 CDS STE2 NC_001138.4 82578 83873 D Ste2p; Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells 82578..83873 Saccharomyces cerevisiae 850518 NP_116628.1 CDS BST1 NC_001138.4 84143 87232 R GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; Bst1p complement(84143..87232) Saccharomyces cerevisiae 850519 NP_116629.1 CDS EPL1 NC_001138.4 87845 90343 R Epl1p; Component of NuA4, which is an essential histone H4/H2A acetyltransferase complex; homologous to Drosophila Enhancer of Polycomb complement(87845..90343) Saccharomyces cerevisiae 850520 NP_683715.1 CDS BUD27 NC_001138.4 90984 93374 D Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud27p 90984..93374 Saccharomyces cerevisiae 850521 NP_116631.1 CDS FRS2 NC_001138.4 93497 95008 R Frs2p; Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar complement(93497..95008) Saccharomyces cerevisiae 850522 NP_116632.1 CDS GAT1 NC_001138.4 95964 97496 D Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; Gat1p 95964..97496 Saccharomyces cerevisiae 850523 NP_116633.1 CDS PAU5 NC_001138.4 99225 99593 R Pau5p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme complement(99225..99593) Saccharomyces cerevisiae 850524 NP_116635.1 CDS LPD1 NC_001138.4 101622 103121 R Lpd1p; Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes complement(101622..103121) Saccharomyces cerevisiae 850527 NP_116636.1 CDS SMX2 NC_001138.4 103693 103926 D Smx2p; Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G 103693..103926 Saccharomyces cerevisiae 850528 NP_116637.1 CDS GNA1 NC_001138.4 103977 104456 R Gna1p; Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA complement(103977..104456) Saccharomyces cerevisiae 850529 NP_116638.1 CDS MDJ1 NC_001138.4 104695 106230 R Protein involved in folding of mitochondrially synthesized proteins in the mitochondrial matrix; localizes to the mitochondrial inner membrane; member of the DnaJ family of molecular chaperones; Mdj1p complement(104695..106230) Saccharomyces cerevisiae 850530 NP_116640.1 CDS HSP12 NC_001138.4 107250 107579 D Plasma membrane localized protein that protects membranes from desiccation; induced by heat shock, oxidative stress, osmostress, stationary phase entry, glucose depletion, oleate and alcohol; regulated by the HOG and Ras-Pka pathways; Hsp12p 107250..107579 Saccharomyces cerevisiae 850532 NP_116641.1 CDS IES1 NC_001138.4 107846 109924 R Subunit of the INO80 chromatin remodeling complex; Ies1p complement(107846..109924) Saccharomyces cerevisiae 850534 NP_116643.1 CDS YFL012W NC_001138.4 110641 111087 D Yfl012wp; Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin 110641..111087 Saccharomyces cerevisiae 850535 NP_116644.1 CDS HXT10 NC_001138.4 112339 113979 D Putative hexose transporter, expressed at low levels and expression is repressed by glucose; Hxt10p 112339..113979 Saccharomyces cerevisiae 850536 NP_116645.1 CDS AUA1 NC_001138.4 114984 115268 D Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease; Aua1p 114984..115268 Saccharomyces cerevisiae 850537 NP_570898.1 CDS WWM1 NC_001138.4 115102 115737 R Wwm1p; WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes Gi phase growth arrest and clonal death that is suppressed by overexpression of MCA1 complement(115102..115737) Saccharomyces cerevisiae 850538 NP_116585.1 CDS CDC4 NC_001138.4 116139 118478 D F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p; Cdc4p 116139..118478 Saccharomyces cerevisiae 850539 NP_116647.1 CDS SMC1 NC_001138.4 119424 123101 D Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure; Smc1p 119424..123101 Saccharomyces cerevisiae 850540 NP_116648.2 CDS BLM10 NC_001138.4 123474 129905 D Proteosome activator subunit; found in association with core particles, with and without the 19S regulatory particle; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200; Blm10p 123474..129905 Saccharomyces cerevisiae 850541 NP_116650.1 CDS SEC4 NC_001138.4 130329 130976 D Secretory vesicle-associated Rab GTPase essential for exocytosis; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane; Sec4p 130329..130976 Saccharomyces cerevisiae 850543 NP_116651.1 CDS VTC2 NC_001138.4 131805 134291 D Vtc2p; Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in protein localization and non-autophagic vacuolar fusion 131805..134291 Saccharomyces cerevisiae 850544 NP_116652.1 CDS MSH4 NC_001138.4 134516 137152 R Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein; Msh4p complement(134516..137152) Saccharomyces cerevisiae 850545 NP_116653.1 CDS YFL002W-A NC_001138.4 138199 143512 D Yfl002w-ap; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(138199..139491,139493..143512) Saccharomyces cerevisiae 850548 NP_058157.1 CDS YFL002W-B NC_001138.4 138199 139515 D Yfl002w-bp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 138199..139515 Saccharomyces cerevisiae 850547 NP_116654.1 CDS SPB4 NC_001138.4 145109 146929 R Spb4p; Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients complement(145109..146929) Saccharomyces cerevisiae 850549 NP_116655.1 CDS DEG1 NC_001138.4 147126 148454 D Non-essential tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; Deg1p 147126..148454 Saccharomyces cerevisiae 850550 NP_116656.1 CDS LOC1 NC_001138.4 149105 149719 D Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; Loc1p 149105..149719 Saccharomyces cerevisiae 850551 NP_116657.1 CDS NIC96 NC_001138.4 150011 152530 D Nic96p; Component of the nuclear pore complex, required for nuclear pore formation; forms a subcomplex with Nsp1p, Nup57p, and Nup49p 150011..152530 Saccharomyces cerevisiae 850552 NP_116658.1 CDS YPI1 NC_001138.4 152652 153119 R Ypi1p; Inhibitor of the type I protein phosphatase Glc7p, which is involved in regulation of a variety of metabolic processes; overproduction causes decreased cellular content of glycogen complement(152652..153119) Saccharomyces cerevisiae 850553 NP_116659.1 CDS RPN11 NC_001138.4 153388 154308 D Rpn11p; Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates 153388..154308 Saccharomyces cerevisiae 850554 NP_116660.1 CDS SAD1 NC_001138.4 154522 155868 R Sad1p; Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle complement(154522..155868) Saccharomyces cerevisiae 850555 NP_116661.1 CDS YFR006W NC_001138.4 156139 157746 D Yfr006wp; Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene 156139..157746 Saccharomyces cerevisiae 850556 NP_116662.1 CDS YFH7 NC_001138.4 159293 160354 D Yfh7p; Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) 159293..160354 Saccharomyces cerevisiae 850558 NP_116663.1 CDS FAR7 NC_001138.4 160529 161194 D Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; Far7p 160529..161194 Saccharomyces cerevisiae 850559 NP_116664.1 CDS GCN20 NC_001138.4 162482 164740 D Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Gcn20p 162482..164740 Saccharomyces cerevisiae 850561 NP_116665.1 CDS UBP6 NC_001138.4 165060 166559 D Ubp6p; Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds 165060..166559 Saccharomyces cerevisiae 850562 NP_116666.1 CDS AIM13 NC_001138.4 166739 167251 R Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria; null mutant displays increased frequency of mitochondrial genome loss (petite formation) and reduced growth rate in minimal glycerol media; Aim13p complement(166739..167251) Saccharomyces cerevisiae 850563 NP_116667.1 CDS YFR012W NC_001138.4 167881 168489 D Yfr012wp; Putative protein of unknown function 167881..168489 Saccharomyces cerevisiae 850565 NP_076889.1 CDS YFR012W-A NC_001138.4 169216 169302 D Yfr012w-ap; Putative protein of unknown function; identified by homology 169216..169302 Saccharomyces cerevisiae 850566 NP_116668.1 CDS IOC3 NC_001138.4 169914 172277 D Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing; Ioc3p 169914..172277 Saccharomyces cerevisiae 850567 NP_116669.1 CDS CMK1 NC_001138.4 172529 173869 R Cmk1p; Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian Cam Kinase II complement(172529..173869) Saccharomyces cerevisiae 850568 NP_116670.1 CDS GSY1 NC_001138.4 174257 176383 R Gsy1p; Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase complement(174257..176383) Saccharomyces cerevisiae 850569 NP_116671.1 CDS YFR016C NC_001138.4 177034 180735 R Yfr016cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene complement(177034..180735) Saccharomyces cerevisiae 850570 NP_116672.1 CDS YFR017C NC_001138.4 182262 182849 R Yfr017cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YFR017C is not an essential gene complement(182262..182849) Saccharomyces cerevisiae 850572 NP_116673.1 CDS YFR018C NC_001138.4 183119 184210 R Yfr018cp; Putative protein of unknown function complement(183119..184210) Saccharomyces cerevisiae 850573 NP_116674.1 CDS FAB1 NC_001138.4 184490 191326 D Fab1p; 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis 184490..191326 Saccharomyces cerevisiae 850574 NP_444297.1 CDS ATG18 NC_001138.4 194800 196302 D Phosphoinositide binding protein required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; Atg18p 194800..196302 Saccharomyces cerevisiae 850577 NP_116677.1 CDS ROG3 NC_001138.4 196821 199022 D Rog3p; Protein that binds to Rsp5p, which is a hect-type ubiquitin ligase, via its 2 PY motifs; has similarity to Rod1p; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant 196821..199022 Saccharomyces cerevisiae 850578 NP_116678.1 CDS PES4 NC_001138.4 199862 201697 D Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation, similar to Mip6p; Pes4p 199862..201697 Saccharomyces cerevisiae 850579 NP_219497.1 CDS LSB3 NC_001138.4 201948 203421 R Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; Lsb3p complement(join(201948..203256,203375..203421)) Saccharomyces cerevisiae 850580 NP_116681.1 CDS HIS2 NC_001138.4 203731 204738 R Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control; His2p complement(203731..204738) Saccharomyces cerevisiae 850581 NP_116682.1 CDS YFR026C NC_001138.4 205736 206245 R Yfr026cp; Putative protein of unknown function complement(205736..206245) Saccharomyces cerevisiae 850583 NP_116683.1 CDS ECO1 NC_001138.4 207440 208285 D Eco1p; Acetyltransferase required for the establishment of sister chromatid cohesion during DNA replication and in response to double-strand breaks; also required for postreplicative double-strand break repair 207440..208285 Saccharomyces cerevisiae 850584 NP_116684.1 CDS CDC14 NC_001138.4 208401 210056 R Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; Cdc14p complement(208401..210056) Saccharomyces cerevisiae 850585 NP_116685.1 CDS PTR3 NC_001138.4 210925 212961 D Ptr3p; Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes 210925..212961 Saccharomyces cerevisiae 850587 NP_116686.1 CDS MET10 NC_001138.4 213300 216407 D Subunit alpha of assimilatory sulfite reductase, which is responsible for the conversion of sulfite into sulfide; Met10p 213300..216407 Saccharomyces cerevisiae 850588 NP_116687.1 CDS SMC2 NC_001138.4 216582 220094 R Smc2p; Component of the condensin complex, essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA, possibly in the cleft formed by the coiled-coil of the folded dimer complement(216582..220094) Saccharomyces cerevisiae 850589 NP_116688.1 CDS RPL2A NC_001138.4 220495 221406 R Rpl2ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins complement(join(220495..221255,221403..221406)) Saccharomyces cerevisiae 850590 NP_116689.1 CDS YFR032C NC_001138.4 222078 222947 R Yfr032cp; Putative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene complement(222078..222947) Saccharomyces cerevisiae 850591 NP_116690.1 CDS RPL29 NC_001138.4 223246 223425 R Rpl29p; Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein; not essential for translation, but required for proper joining of the large and small ribosomal subunits and for normal translation rate complement(223246..223425) Saccharomyces cerevisiae 850592 NP_878072.1 CDS YFR032C-B NC_001138.4 223698 223961 R Yfr032c-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(223698..223961) Saccharomyces cerevisiae 1466402 NP_116691.1 CDS QCR6 NC_001138.4 224314 224757 R Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; Qcr6p complement(224314..224757) Saccharomyces cerevisiae 850593 NP_116692.1 CDS PHO4 NC_001138.4 225008 225946 R Pho4p; Basic helix-loop-helix (bHLH) transcription factor of the myc-family; binds cooperatively with Pho2p to the PHO5 promoter; function is regulated by phosphorylation at multiple sites and by phosphate availability complement(225008..225946) Saccharomyces cerevisiae 850594 NP_116693.1 CDS YFR035C NC_001138.4 226109 226453 R Yfr035cp; Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein complement(226109..226453) Saccharomyces cerevisiae 850595 NP_116694.1 CDS CDC26 NC_001138.4 226950 227324 D Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Cdc26p 226950..227324 Saccharomyces cerevisiae 850597 NP_116695.1 CDS RSC8 NC_001138.4 227500 229173 R Rsc8p; Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters complement(227500..229173) Saccharomyces cerevisiae 850598 NP_116696.2 CDS IRC5 NC_001138.4 229367 231928 D Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52 foci; Irc5p 229367..231928 Saccharomyces cerevisiae 850599 NP_116697.1 CDS YFR039C NC_001138.4 231999 233531 R Yfr039cp; Putative protein of unknown function; YFR039C is not an essential gene complement(231999..233531) Saccharomyces cerevisiae 850600 NP_116698.2 CDS SAP155 NC_001138.4 234229 237237 D Sap155p; Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p 234229..237237 Saccharomyces cerevisiae 850601 NP_116699.1 CDS ERJ5 NC_001138.4 237355 238242 R Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response; Erj5p complement(237355..238242) Saccharomyces cerevisiae 850602 NP_116700.1 CDS KEG1 NC_001138.4 238445 239047 D Protein of unknown function required for cell viability; localizes to the endoplasmic reticulum membrane; Keg1p 238445..239047 Saccharomyces cerevisiae 850603 NP_116701.1 CDS IRC6 NC_001138.4 239101 239814 R hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci; Irc6p complement(239101..239814) Saccharomyces cerevisiae 850604 NP_116702.1 CDS DUG1 NC_001138.4 239979 241424 R Probable di- and tri-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); Dug1p complement(239979..241424) Saccharomyces cerevisiae 850605 NP_116703.3 CDS YFR045W NC_001138.4 241985 242986 D Yfr045wp; Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white join(241985..241996,242069..242986) Saccharomyces cerevisiae 850606 NP_116704.1 CDS CNN1 NC_001138.4 243061 244146 R Kinetochore protein of unknown function; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by both Clb5-Cdk1 and, to a lesser extent, Clb2-Cdk1.; Cnn1p complement(243061..244146) Saccharomyces cerevisiae 850607 NP_602317.1 CDS BNA6 NC_001138.4 244266 245153 R Quinolinate phosphoribosyl transferase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; Bna6p complement(244266..245153) Saccharomyces cerevisiae 850608 NP_683716.1 CDS RMD8 NC_001138.4 246133 248121 D Rmd8p; Cytosolic protein required for sporulation 246133..248121 Saccharomyces cerevisiae 850609 NP_116707.1 CDS YMR31 NC_001138.4 248510 248881 D Ymr31p; Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 248510..248881 Saccharomyces cerevisiae 850610 NP_116708.1 CDS PRE4 NC_001138.4 249053 249853 R Pre4p; 20S proteasome beta-type subunit complement(249053..249853) Saccharomyces cerevisiae 850611 NP_116709.1 CDS RET2 NC_001138.4 250150 251790 R Ret2p; Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER complement(250150..251790) Saccharomyces cerevisiae 850612 NP_116710.1 CDS RPN12 NC_001138.4 252492 253316 D Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; Rpn12p 252492..253316 Saccharomyces cerevisiae 850613 NP_116711.1 CDS HXK1 NC_001138.4 253579 255036 R Hxk1p; Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p complement(253579..255036) Saccharomyces cerevisiae 850614 NP_116714.1 CDS IRC7 NC_001138.4 264191 265213 D Putative cystathionine beta-lyase; involved in copper ion homeostasis and sulfur metabolism; null mutant displays increased levels of spontaneous Rad52 foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Irc7p 264191..265213 Saccharomyces cerevisiae 850616 NP_116715.1 CDS YFR057W NC_001138.4 269048 269503 D Yfr057wp; Putative protein of unknown function 269048..269503 Saccharomyces cerevisiae 850618 NP_011251.1 CDS COS12 NC_001139.8 2790 3932 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos12p 2790..3932 Saccharomyces cerevisiae 852628 NP_011252.1 CDS YGL262W NC_001139.8 5312 5839 D Ygl262wp; Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene 5312..5839 Saccharomyces cerevisiae 852629 NP_011253.1 CDS PAU11 NC_001139.8 6290 6652 R Putative protein of unknown function; mRNA expression appears to be regulated by SUT1 and UPC2; Pau11p complement(6290..6652) Saccharomyces cerevisiae 852630 NP_011254.1 CDS YGL260W NC_001139.8 6860 7090 D Ygl260wp; Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium 6860..7090 Saccharomyces cerevisiae 852631 NP_011255.1 CDS YPS5 NC_001139.8 8470 8967 D Yps5p; Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p 8470..8967 Saccharomyces cerevisiae 852632 NP_076890.1 CDS YGL258W-A NC_001139.8 9162 9395 D Ygl258w-ap; Putative protein of unknown function 9162..9395 Saccharomyces cerevisiae 852633 NP_011256.1 CDS VEL1 NC_001139.8 11110 11730 D Vel1p; Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants 11110..11730 Saccharomyces cerevisiae 852634 NP_011257.1 CDS MNT2 NC_001139.8 12481 14157 R Mnt2p; Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans complement(12481..14157) Saccharomyces cerevisiae 852635 NP_011258.1 CDS ADH4 NC_001139.8 14910 16307 D Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency; Adh4p 14910..16307 Saccharomyces cerevisiae 852636 NP_011259.1 CDS ZRT1 NC_001139.8 20978 22108 D Zrt1p; High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor 20978..22108 Saccharomyces cerevisiae 852637 NP_011260.1 CDS FZF1 NC_001139.8 22304 23203 D Transcription factor involved in sulfite metabolism, sole identified regulatory target is SSU1, overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; Fzf1p 22304..23203 Saccharomyces cerevisiae 852638 NP_011261.1 CDS HXK2 NC_001139.8 23935 25395 D Hxk2p; Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene 23935..25395 Saccharomyces cerevisiae 852639 NP_011262.1 CDS RTG2 NC_001139.8 25718 27484 R Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p; Rtg2p complement(25718..27484) Saccharomyces cerevisiae 852640 NP_011263.2 CDS HFM1 NC_001139.8 27921 31636 R Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity; Hfm1p complement(join(27921..31426,31579..31636)) Saccharomyces cerevisiae 852641 NP_011264.2 CDS RMR1 NC_001139.8 31910 32635 D Rmr1p; Putative protein of unknown function; deletion mutant results in reduced PIS1 expression and has growth defects on non-fermentable carbon sources and on minimal media; GFP-fusion protein localizes to both the cytoplasm and the nucleus 31910..32635 Saccharomyces cerevisiae 852642 NP_011265.1 CDS ZIP2 NC_001139.8 33098 35212 D Zip2p; Meiosis-specific protein involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis 33098..35212 Saccharomyces cerevisiae 852643 NP_011266.1 CDS PDE1 NC_001139.8 35653 36762 D Pde1p; Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation 35653..36762 Saccharomyces cerevisiae 852644 NP_011267.1 CDS BRR6 NC_001139.8 36933 37526 D Essential nuclear envelope integral membrane protein required for nuclear transport; depletion alters nucleoporin distribution and nuclear envelope morphology, suggesting a role in the spatial organization of nuclear pores; Brr6p 36933..37526 Saccharomyces cerevisiae 852645 NP_011268.1 CDS RAI1 NC_001139.8 37617 38780 R Rai1p; Nuclear protein that binds to and stabilizes the exoribonuclease Rat1p, required for pre-rRNA processing complement(37617..38780) Saccharomyces cerevisiae 852646 NP_011269.2 CDS GUS1 NC_001139.8 39023 41149 D Gus1p; Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm 39023..41149 Saccharomyces cerevisiae 852606 NP_011270.1 CDS RTF1 NC_001139.8 41498 43174 D Subunit of the RNA polymerase II-associated Paf1 complex; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in telomere maintenance; Rtf1p 41498..43174 Saccharomyces cerevisiae 852607 NP_011271.1 CDS TAD1 NC_001139.8 43307 44509 D tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala; Tad1p 43307..44509 Saccharomyces cerevisiae 852608 NP_011272.1 CDS YGL242C NC_001139.8 44652 45197 R Ygl242cp; Putative protein of unknown function; deletion mutant is viable complement(44652..45197) Saccharomyces cerevisiae 852609 NP_011273.1 CDS KAP114 NC_001139.8 45445 48459 D Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; Kap114p 45445..48459 Saccharomyces cerevisiae 852610 NP_011274.2 CDS DOC1 NC_001139.8 48613 49365 D Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; Doc1p 48613..49365 Saccharomyces cerevisiae 852611 NP_011276.1 CDS CSE1 NC_001139.8 49552 52434 D Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation; Cse1p 49552..52434 Saccharomyces cerevisiae 852612 NP_011277.1 CDS HAP2 NC_001139.8 52731 53528 R Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; Hap2p complement(52731..53528) Saccharomyces cerevisiae 852614 NP_011278.2 CDS MTO1 NC_001139.8 53787 55796 R Mto1p; Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants complement(53787..55796) Saccharomyces cerevisiae 852616 NP_011279.1 CDS YGL235W NC_001139.8 55279 55815 D Ygl235wp; Putative protein of unknown function; potential Cdc28p substrate 55279..55815 Saccharomyces cerevisiae 852615 NP_011280.1 CDS ADE5,7 NC_001139.8 56482 58890 D Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities; Ade5,7p 56482..58890 Saccharomyces cerevisiae 852617 NP_011281.1 CDS SEC15 NC_001139.8 59122 61854 D Sec15p; Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which mediates polarized targeting of vesicles to active sites of exocytosis; Sec15p associates with Sec4p and vesicles 59122..61854 Saccharomyces cerevisiae 852618 NP_011282.1 CDS TAN1 NC_001139.8 62075 63002 D Tan1p; Putative tRNA acetyltransferase, RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA join(62075..62131,62190..63002) Saccharomyces cerevisiae 852619 NP_011283.1 CDS YGL231C NC_001139.8 63048 63620 R Ygl231cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum complement(63048..63620) Saccharomyces cerevisiae 852620 NP_011284.1 CDS YGL230C NC_001139.8 63772 64215 R Ygl230cp; Putative protein of unknown function; non-essential gene complement(63772..64215) Saccharomyces cerevisiae 852621 NP_011285.1 CDS SAP4 NC_001139.8 64503 66959 R Sap4p; Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p complement(64503..66959) Saccharomyces cerevisiae 852622 NP_011286.1 CDS SHE10 NC_001139.8 67598 69331 D She10p; Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest 67598..69331 Saccharomyces cerevisiae 852623 NP_011287.1 CDS VID30 NC_001139.8 69671 72547 D Vid30p; Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); shifts the balance of nitrogen metabolism toward the production of glutamate; localized to the nucleus and the cytoplasm 69671..72547 Saccharomyces cerevisiae 852624 NP_011288.1 CDS OST5 NC_001139.8 72748 73157 R Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost5p complement(join(72748..72987,73137..73157)) Saccharomyces cerevisiae 852625 NP_011289.1 CDS YGL226W NC_001139.8 73339 73710 D Ygl226wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 73339..73710 Saccharomyces cerevisiae 852626 NP_011290.1 CDS VRG4 NC_001139.8 76893 77906 D Vrg4p; Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi 76893..77906 Saccharomyces cerevisiae 852647 NP_011291.1 CDS SDT1 NC_001139.8 78014 78856 R Sdt1p; Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives complement(78014..78856) Saccharomyces cerevisiae 852648 NP_011292.1 CDS COG1 NC_001139.8 79111 80364 R Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog1p complement(79111..80364) Saccharomyces cerevisiae 852649 NP_011293.1 CDS EDC1 NC_001139.8 80658 81185 R RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; Edc1p complement(80658..81185) Saccharomyces cerevisiae 852650 NP_011294.1 CDS NIF3 NC_001139.8 81425 82291 R Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Nif3p complement(81425..82291) Saccharomyces cerevisiae 852651 NP_011295.1 CDS AIM15 NC_001139.8 82373 82735 D Putative protein of unknown function; (GFP)-fusion protein localizes to the cytoplasm and the nucleus; null mutant displays decreased mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim15p 82373..82735 Saccharomyces cerevisiae 852652 NP_011296.1 CDS MDM34 NC_001139.8 82877 84256 R Mitochondrial outer membrane protein, required for normal mitochondrial morphology and inheritance; localizes to dots on the mitochondrial surface near mtDNA nucleoids; interacts genetically with MDM31 and MDM32; Mdm34p complement(82877..84256) Saccharomyces cerevisiae 852654 NP_011299.1 CDS KIP3 NC_001139.8 84884 87301 D Kip3p; Kinesin-related motor protein involved in mitotic spindle positioning 84884..87301 Saccharomyces cerevisiae 852655 NP_011300.1 CDS CLG1 NC_001139.8 87980 89338 D Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2; Clg1p 87980..89338 Saccharomyces cerevisiae 852657 NP_011302.1 CDS SKI8 NC_001139.8 90058 91251 R Ski8p; Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic recombination; required for repressing propagation of dsRNA viruses complement(90058..91251) Saccharomyces cerevisiae 852659 NP_011303.1 CDS VAM7 NC_001139.8 91436 92386 D Vam7p; Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; functions with a syntaxin homolog Vam3p in vacuolar protein trafficking 91436..92386 Saccharomyces cerevisiae 852660 NP_011304.2 CDS NCS6 NC_001139.8 92516 93595 D Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae; Ncs6p 92516..93595 Saccharomyces cerevisiae 852661 NP_011305.1 CDS YPT32 NC_001139.8 93797 94465 D Ypt32p; GTPase of the Ypt/Rab family, very similar to Ypt31p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi 93797..94465 Saccharomyces cerevisiae 852662 NP_011306.1 CDS MIG2 NC_001139.8 95862 97010 D Protein containing zinc fingers, involved in repression, along with Mig1p, of SUC2 (invertase) expression by high levels of glucose; binds to Mig1p-binding sites in SUC2 promoter; Mig2p 95862..97010 Saccharomyces cerevisiae 852663 NP_011307.1 CDS SIP2 NC_001139.8 97342 98589 D Sip2p; One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane 97342..98589 Saccharomyces cerevisiae 852664 NP_011308.1 CDS SPT16 NC_001139.8 98973 102080 D Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), facilitates RNA Polymerase II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure; Spt16p 98973..102080 Saccharomyces cerevisiae 852665 NP_011309.1 CDS CHC1 NC_001139.8 102547 107508 R Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function; Chc1p complement(102547..107508) Saccharomyces cerevisiae 852666 NP_011310.1 CDS POX1 NC_001139.8 108162 110408 D Pox1p; Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix 108162..110408 Saccharomyces cerevisiae 852667 NP_011312.1 CDS KEX1 NC_001139.8 112479 114668 R Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins; Kex1p complement(112479..114668) Saccharomyces cerevisiae 852670 NP_011313.1 CDS ARO8 NC_001139.8 116063 117565 D Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis; Aro8p 116063..117565 Saccharomyces cerevisiae 852672 NP_011314.2 CDS MCM6 NC_001139.8 117858 120911 R Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; Mcm6p complement(117858..120911) Saccharomyces cerevisiae 852673 NP_011315.1 CDS EMP24 NC_001139.8 122699 123310 R Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport; Emp24p complement(122699..123310) Saccharomyces cerevisiae 852675 NP_011317.2 CDS YIP4 NC_001139.8 123596 124303 D Yip4p; Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport 123596..124303 Saccharomyces cerevisiae 852676 NP_011318.1 CDS MDS3 NC_001139.8 124703 129166 D Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; Mds3p 124703..129166 Saccharomyces cerevisiae 852678 NP_011319.2 CDS DSD1 NC_001139.8 129888 131174 D Dsd1p; D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia; specifc for D-serine, unlike the bacterial enzyme which recognizes both D-serine and L-serine as substrates 129888..131174 Saccharomyces cerevisiae 852679 NP_011320.1 CDS GCN1 NC_001139.8 131531 139549 D Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Gcn1p 131531..139549 Saccharomyces cerevisiae 852680 NP_001032578.1 CDS YGL194C-A NC_001139.8 139725 139967 R Ygl194c-ap; Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species complement(139725..139967) Saccharomyces cerevisiae 3799973 NP_011321.1 CDS HOS2 NC_001139.8 140374 141732 R Hos2p; Histone deacetylase required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity complement(140374..141732) Saccharomyces cerevisiae 852681 NP_011322.1 CDS YGL193C NC_001139.8 141922 142233 R Ygl193cp; Haploid-specific gene repressed by a1-alpha2, turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold complement(141922..142233) Saccharomyces cerevisiae 852682 NP_011323.1 CDS IME4 NC_001139.8 142252 144054 D Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells; Ime4p 142252..144054 Saccharomyces cerevisiae 852683 NP_011324.1 CDS COX13 NC_001139.8 144814 145203 D Subunit VIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; Cox13p 144814..145203 Saccharomyces cerevisiae 852684 NP_011325.1 CDS CDC55 NC_001139.8 145815 147395 R Non-essential regulatory subunit B of protein phosphatase 2A, which has multiple roles in mitosis and protein biosynthesis; involved in regulation of mitotic exit; found in the nucleus of most cells, also at the bud neck and at the bud tip; Cdc55p complement(145815..147395) Saccharomyces cerevisiae 852685 NP_011326.1 CDS RPS26A NC_001139.8 148235 148594 R Rps26ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similarity to rat S26 ribosomal protein complement(148235..148594) Saccharomyces cerevisiae 852686 NP_878073.1 CDS YGL188C-A NC_001139.8 148830 148970 R Ygl188c-ap; Putative protein of unknown function complement(148830..148970) Saccharomyces cerevisiae 1466453 NP_011328.1 CDS COX4 NC_001139.8 149710 150177 R Subunit IV of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; N-terminal 25 residues of precursor are cleaved during mitochondrial import; phosphorylated; Cox4p complement(149710..150177) Saccharomyces cerevisiae 852688 NP_011329.1 CDS TPN1 NC_001139.8 151043 152782 R Tpn1p; Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p complement(151043..152782) Saccharomyces cerevisiae 852689 NP_011330.1 CDS YGL185C NC_001139.8 153173 154312 R Ygl185cp; Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(153173..154312) Saccharomyces cerevisiae 852690 NP_011331.1 CDS STR3 NC_001139.8 154621 156018 R Str3p; Cystathionine beta-lyase, converts cystathionine into homocysteine complement(154621..156018) Saccharomyces cerevisiae 852691 NP_011332.2 CDS MND1 NC_001139.8 156549 157291 R Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks; Mnd1p complement(join(156549..157205,157289..157291)) Saccharomyces cerevisiae 852692 NP_011334.1 CDS GTS1 NC_001139.8 157912 159102 D Protein containing a zinc-finger in the N-terminus and a long Gln-rich region in the C-terminus; regulates ultradian rhythm, cell size, cell cycle, lifespan, sporulation, heat tolerance, and multidrug transport; Gts1p 157912..159102 Saccharomyces cerevisiae 852694 NP_011335.1 CDS ATG1 NC_001139.8 160071 162764 D Protein serine/threonine kinase, required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for pre-autophagosome formation; forms a complex with Atg13p and Atg17p; Atg1p 160071..162764 Saccharomyces cerevisiae 852695 NP_011336.1 CDS TOS3 NC_001139.8 163415 165097 R Tos3p; Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome complement(163415..165097) Saccharomyces cerevisiae 852696 NP_011337.1 CDS MPT5 NC_001139.8 167358 170577 D Mpt5p; Member of the Puf family of RNA-binding proteins; binds to mRNAs encoding chromatin modifiers and spindle pole body components; involved in longevity, maintenance of cell wall integrity, and sensitivity to and recovery from pheromone arrest join(167358..167360,168001..170577) Saccharomyces cerevisiae 852697 NP_011339.1 CDS YGL176C NC_001139.8 171421 173085 R Ygl176cp; Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype complement(171421..173085) Saccharomyces cerevisiae 852699 NP_011340.1 CDS SAE2 NC_001139.8 173291 174328 R Sae2p; Protein with a role in accurate meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents complement(173291..174328) Saccharomyces cerevisiae 852700 NP_011341.1 CDS BUD13 NC_001139.8 174551 175351 D Subunit of the RES complex, which is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern; Bud13p 174551..175351 Saccharomyces cerevisiae 852701 NP_011342.1 CDS KEM1 NC_001139.8 175533 180119 R Kem1p; Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved in mRNA decay; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance complement(175533..180119) Saccharomyces cerevisiae 852702 NP_011343.1 CDS NUP49 NC_001139.8 180706 182124 D Subunit of the Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of the nuclear pore complex (NPC), required for nuclear export of ribosomes; Nup49p 180706..182124 Saccharomyces cerevisiae 852703 NP_011344.1 CDS ROK1 NC_001139.8 182396 184090 D Rok1p; ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis 182396..184090 Saccharomyces cerevisiae 852704 NP_011345.1 CDS SPO74 NC_001139.8 184159 185400 R Spo74p; Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation complement(184159..185400) Saccharomyces cerevisiae 852705 NP_011346.1 CDS SUA5 NC_001139.8 186065 187345 D Sua5p; Protein required for respiratory growth; null mutation suppresses the Cyc1p translation defect caused by the presence of an aberrant ATG codon upstream of the correct start 186065..187345 Saccharomyces cerevisiae 852707 NP_011347.1 CDS HUR1 NC_001139.8 187470 187802 D Protein required for hydroxyurea resistance; has possible roles in DNA replication and maintenance of proper telomere length; Hur1p 187470..187802 Saccharomyces cerevisiae 852708 NP_011348.1 CDS PMR1 NC_001139.8 187622 190474 R Pmr1p; High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease complement(187622..190474) Saccharomyces cerevisiae 852709 NP_011349.1 CDS CUP2 NC_001139.8 191135 191812 D Cup2p; Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations 191135..191812 Saccharomyces cerevisiae 852710 NP_011351.1 CDS YRB30 NC_001139.8 191984 193306 R Yrb30p; RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes complement(191984..193306) Saccharomyces cerevisiae 852712 NP_011352.1 CDS RAD54 NC_001139.8 193713 196409 R Rad54p; DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family complement(193713..196409) Saccharomyces cerevisiae 852713 NP_011353.1 CDS SUT1 NC_001139.8 198144 199043 D Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; Sut1p 198144..199043 Saccharomyces cerevisiae 852714 NP_011354.1 CDS YIP5 NC_001139.8 199216 200148 R Yip5p; Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport complement(199216..200148) Saccharomyces cerevisiae 852715 NP_011355.1 CDS AIM14 NC_001139.8 200567 202279 D Putative protein of unknown function; similar to iron/copper reductases (FRE1-8), possibly involved in iron homeostasis; may interact with ribosomes, null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim14p 200567..202279 Saccharomyces cerevisiae 852716 NP_011356.1 CDS YGL159W NC_001139.8 202727 203839 D Ygl159wp; Putative protein of unknown function; deletion mutant has no detectable phenotype 202727..203839 Saccharomyces cerevisiae 852717 NP_011357.1 CDS RCK1 NC_001139.8 207038 208576 D Rck1p; Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations 207038..208576 Saccharomyces cerevisiae 852719 NP_011358.1 CDS YGL157W NC_001139.8 209011 210054 D Ygl157wp; Oxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) 209011..210054 Saccharomyces cerevisiae 852720 NP_011359.1 CDS AMS1 NC_001139.8 210421 213672 D Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway; Ams1p 210421..213672 Saccharomyces cerevisiae 852721 NP_011360.1 CDS CDC43 NC_001139.8 214086 215216 D Cdc43p; Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis 214086..215216 Saccharomyces cerevisiae 852722 NP_011361.1 CDS LYS5 NC_001139.8 215283 216101 R Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine; Lys5p complement(215283..216101) Saccharomyces cerevisiae 852723 NP_011362.1 CDS PEX14 NC_001139.8 216278 217303 D Pex14p; Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p 216278..217303 Saccharomyces cerevisiae 852724 NP_011364.1 CDS NUT1 NC_001139.8 217529 220927 D Nut1p; Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription 217529..220927 Saccharomyces cerevisiae 852726 NP_011365.1 CDS INO80 NC_001139.8 221109 225578 R Ino80p; ATPase that forms a large complex, containing actin and several actin-related proteins, that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; shows similarity to the Snf2p family of ATPases complement(221109..225578) Saccharomyces cerevisiae 852728 NP_011367.1 CDS ARO2 NC_001139.8 226404 227534 D Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; Aro2p 226404..227534 Saccharomyces cerevisiae 852729 NP_011368.1 CDS RPL9A NC_001139.8 227759 228334 R Rpl9ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins complement(227759..228334) Saccharomyces cerevisiae 852730 NP_011369.1 CDS YGL146C NC_001139.8 228756 229691 R Ygl146cp; Putative protein of unknown function, contains two putative transmembrane spans, shows no significant homology to any other known protein sequence, YGL146C is not an essential gene complement(228756..229691) Saccharomyces cerevisiae 852731 NP_116577.1 CDS TIP20 NC_001139.8 230248 232353 D Tip20p; Peripheral membrane protein required for fusion of COPI vesicles with the ER, prohibits back-fusion of COPII vesicles with the ER, may act as a sensor for vesicles at the ER membrane; interacts with Sec20p 230248..232353 Saccharomyces cerevisiae 852732 NP_011371.1 CDS ROG1 NC_001139.8 232455 234512 R Rog1p; Protein with putative serine active lipase domain complement(232455..234512) Saccharomyces cerevisiae 852733 NP_011372.1 CDS MRF1 NC_001139.8 234722 235963 R Mrf1p; Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability complement(234722..235963) Saccharomyces cerevisiae 852734 NP_011373.1 CDS GPI10 NC_001139.8 236274 238124 R Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog; Gpi10p complement(236274..238124) Saccharomyces cerevisiae 852735 NP_011374.1 CDS HUL5 NC_001139.8 238358 241090 D Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability; Hul5p 238358..241090 Saccharomyces cerevisiae 852736 NP_011375.1 CDS YGL140C NC_001139.8 241358 245017 R Ygl140cp; Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains complement(241358..245017) Saccharomyces cerevisiae 852737 NP_011376.1 CDS FLC3 NC_001139.8 245721 248129 D Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; Flc3p 245721..248129 Saccharomyces cerevisiae 852738 NP_011377.1 CDS YGL138C NC_001139.8 248499 249536 R Ygl138cp; Putative protein of unknown function; has no significant sequence similarity to any known protein complement(248499..249536) Saccharomyces cerevisiae 852739 NP_011378.1 CDS SEC27 NC_001139.8 249874 252743 D Sec27p; Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP join(249874..249891,250092..252743) Saccharomyces cerevisiae 852740 NP_011379.1 CDS MRM2 NC_001139.8 252902 253864 R Mrm2p; Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ complement(252902..253864) Saccharomyces cerevisiae 852741 NP_011380.1 CDS RPL1B NC_001139.8 254646 255299 D Rpl1bp; N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Ap and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal 254646..255299 Saccharomyces cerevisiae 852742 NP_011381.1 CDS PCL10 NC_001139.8 255668 256969 D Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; Pcl10p 255668..256969 Saccharomyces cerevisiae 852743 NP_011382.1 CDS ITC1 NC_001139.8 257712 261506 D Itc1p; Component of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1 257712..261506 Saccharomyces cerevisiae 852744 NP_011384.1 CDS SNT2 NC_001139.8 261653 265864 R Snt2p; DNA binding protein with similarity to the S. pombe Snt2 protein complement(261653..265864) Saccharomyces cerevisiae 852746 NP_011385.1 CDS CEG1 NC_001139.8 266150 267529 D Ceg1p; Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphophatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 266150..267529 Saccharomyces cerevisiae 852747 NP_011386.1 CDS RSM23 NC_001139.8 267728 269194 R Rsm23p; Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p complement(267728..269194) Saccharomyces cerevisiae 852748 NP_011387.2 CDS CWC23 NC_001139.8 269299 270150 R Cwc23p; Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p complement(269299..270150) Saccharomyces cerevisiae 852749 NP_011388.1 CDS SOH1 NC_001139.8 270398 270781 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits; Soh1p complement(270398..270781) Saccharomyces cerevisiae 852750 NP_011389.1 CDS SCS3 NC_001139.8 271003 272145 D Scs3p; Protein required for inositol prototrophy, identified as an ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; disputed role in the synthesis of inositol phospholipids from inositol 271003..272145 Saccharomyces cerevisiae 852751 NP_011390.2 CDS MET13 NC_001139.8 272526 274328 D Met13p; Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway 272526..274328 Saccharomyces cerevisiae 852752 NP_011391.1 CDS MON1 NC_001139.8 274786 276720 R Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate; Mon1p complement(274786..276720) Saccharomyces cerevisiae 852753 NP_011392.1 CDS RPS2 NC_001139.8 277623 278387 D Rps2p; Protein component of the small (40S) subunit, essential for control of translational accuracy; has similarity to E. coli S5 and rat S2 ribosomal proteins 277623..278387 Saccharomyces cerevisiae 852754 NP_011393.1 CDS NAB2 NC_001139.8 278952 280529 R Nuclear polyadenylated RNA-binding protein; autoregulates mRNA levels; related to human hnRNPs; has nuclear localization signal sequence that binds to Kap104p; required for poly(A) tail length control and nuclear mRNA export; Nab2p complement(278952..280529) Saccharomyces cerevisiae 852755 NP_011394.1 CDS GPG1 NC_001139.8 280783 281163 R Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Grp1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; Gpg1p complement(280783..281163) Saccharomyces cerevisiae 852756 NP_011395.1 CDS PRP43 NC_001139.8 281640 283943 R RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome; Prp43p complement(281640..283943) Saccharomyces cerevisiae 852757 NP_011396.1 CDS ABC1 NC_001139.8 284448 285953 D Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; Abc1p 284448..285953 Saccharomyces cerevisiae 852758 NP_011398.1 CDS YGL117W NC_001139.8 288518 289315 D Ygl117wp; Putative protein of unknown function 288518..289315 Saccharomyces cerevisiae 852761 NP_011399.1 CDS CDC20 NC_001139.8 289812 291644 D Cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C), which is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate; Cdc20p 289812..291644 Saccharomyces cerevisiae 852762 NP_011400.1 CDS SNF4 NC_001139.8 292036 293004 D Snf4p; Activating gamma subunit of the AMP-activated Snf1p kinase complex (contains Snf1p and a Sip1p/Sip2p/Gal83p family member); activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis 292036..293004 Saccharomyces cerevisiae 852763 NP_011401.1 CDS YGL114W NC_001139.8 293463 295640 D Ygl114wp; Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters 293463..295640 Saccharomyces cerevisiae 852764 NP_011402.1 CDS SLD3 NC_001139.8 295935 297941 D Sld3p; Protein involved in the initiation of DNA replication, required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p 295935..297941 Saccharomyces cerevisiae 852765 NP_011403.1 CDS TAF6 NC_001139.8 298181 299731 R Taf6p; Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 complement(298181..299731) Saccharomyces cerevisiae 852766 NP_011404.1 CDS NSA1 NC_001139.8 299981 301372 D Nsa1p; Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis 299981..301372 Saccharomyces cerevisiae 852767 NP_011405.1 CDS CUE3 NC_001139.8 301540 303414 R Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; Cue3p complement(301540..303414) Saccharomyces cerevisiae 852768 NP_011407.1 CDS YGL108C NC_001139.8 303652 304074 R Ygl108cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery complement(303652..304074) Saccharomyces cerevisiae 852770 NP_011408.1 CDS RMD9 NC_001139.8 304336 306276 R Rmd9p; Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes complement(304336..306276) Saccharomyces cerevisiae 852771 NP_011409.1 CDS MLC1 NC_001139.8 306563 307012 D Mlc1p; Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition 306563..307012 Saccharomyces cerevisiae 852772 NP_011410.1 CDS ARC1 NC_001139.8 307440 308570 D Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; Arc1p 307440..308570 Saccharomyces cerevisiae 852773 NP_011411.1 CDS VPS73 NC_001139.8 308716 310176 R Vps73p; Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family complement(308716..310176) Saccharomyces cerevisiae 852774 NP_011412.1 CDS RPL28 NC_001139.8 310970 311930 D Rpl28p; Ribosomal protein of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a ribosomal proteins; may have peptidyl transferase activity; can mutate to cycloheximide resistance join(310970..311018,311530..311930) Saccharomyces cerevisiae 852775 NP_011414.1 CDS YGL101W NC_001139.8 312196 312843 D Ygl101wp; Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p 312196..312843 Saccharomyces cerevisiae 852777 NP_011415.1 CDS SEH1 NC_001139.8 313237 314286 D Seh1p; Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nup120p, Nup145p, and Seh1p); homologous to Sec13p 313237..314286 Saccharomyces cerevisiae 852778 NP_011416.1 CDS LSG1 NC_001139.8 314634 316556 D Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm; Lsg1p 314634..316556 Saccharomyces cerevisiae 852779 NP_011417.1 CDS USE1 NC_001139.8 317345 318082 D Use1p; Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p 317345..318082 Saccharomyces cerevisiae 852780 NP_011418.1 CDS SRM1 NC_001139.8 321785 323233 D Srm1p; Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p 321785..323233 Saccharomyces cerevisiae 852782 NP_011419.1 CDS TOS8 NC_001139.8 325334 326164 D Tos8p; Homeodomain-containing transcription factor; SBF regulated target gene that in turn regulates expression of genes involved in G1/S phase events such as bud site selection, bud emergence and cell cycle progression; similarity to Cup9p 325334..326164 Saccharomyces cerevisiae 852783 NP_011420.1 CDS VPS45 NC_001139.8 328877 330610 R Vps45p; Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment complement(328877..330610) Saccharomyces cerevisiae 852785 NP_011421.1 CDS PAN2 NC_001139.8 331121 334468 R Pan2p; Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes complement(331121..334468) Saccharomyces cerevisiae 852786 NP_011422.1 CDS SPC105 NC_001139.8 334889 337642 D Spc105p; Protein required for accurate chromosome segregation, localizes to the nuclear side of the spindle pole body; forms a complex with Ydr532cp 334889..337642 Saccharomyces cerevisiae 852787 NP_011423.1 CDS NUP145 NC_001139.8 337909 341862 D Nup145p; Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p 337909..341862 Saccharomyces cerevisiae 852788 NP_011424.1 CDS NBP35 NC_001139.8 342059 343045 R Nbp35p; Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases complement(342059..343045) Saccharomyces cerevisiae 852789 NP_011425.1 CDS LIF1 NC_001139.8 343322 344587 D Lif1p; Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein 343322..344587 Saccharomyces cerevisiae 852790 NP_011426.1 CDS MF(ALPHA)2 NC_001139.8 344794 345156 R Mf(alpha)2p; Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 complement(344794..345156) Saccharomyces cerevisiae 852791 NP_011428.1 CDS MMS2 NC_001139.8 346409 346907 R Protein involved in error-free postreplication DNA repair; forms a heteromeric complex with Ubc13p that has a ubiquitin-conjugating activity; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; Mms2p complement(join(346409..346811,346897..346907)) Saccharomyces cerevisiae 852793 NP_011429.1 CDS MAD1 NC_001139.8 347122 349371 D Mad1p; Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p 347122..349371 Saccharomyces cerevisiae 852794 NP_011430.1 CDS YGL085W NC_001139.8 349619 350443 D Ygl085wp; Putative protein of unknown function, has homology to Staphylococcus aureus nuclease; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS 349619..350443 Saccharomyces cerevisiae 852795 NP_011431.1 CDS GUP1 NC_001139.8 350619 352301 R Plasma membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p complement(350619..352301) Saccharomyces cerevisiae 852796 NP_011432.1 CDS SCY1 NC_001139.8 353061 355475 D Scy1p; Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase 353061..355475 Saccharomyces cerevisiae 852797 NP_011433.1 CDS YGL082W NC_001139.8 355830 356975 D Ygl082wp; Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene 355830..356975 Saccharomyces cerevisiae 852798 NP_011434.1 CDS YGL081W NC_001139.8 357380 358342 D Ygl081wp; Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis 357380..358342 Saccharomyces cerevisiae 852799 NP_011435.1 CDS FMP37 NC_001139.8 358639 359031 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp37p 358639..359031 Saccharomyces cerevisiae 852800 NP_011436.1 CDS YGL079W NC_001139.8 359447 360103 D Ygl079wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome; identified as a transcriptional activator in a high-throughput yeast one-hybrid assay 359447..360103 Saccharomyces cerevisiae 852801 NP_011437.1 CDS DBP3 NC_001139.8 360291 361862 R Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; Dbp3p complement(360291..361862) Saccharomyces cerevisiae 852802 NP_011438.1 CDS HNM1 NC_001139.8 362228 363919 R Hnm1p; Choline/ethanolamine transporter (permease) that also controls the uptake of nitrogen mustard; expression is co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol complement(362228..363919) Saccharomyces cerevisiae 852803 NP_011439.1 CDS RPL7A NC_001139.8 364338 365999 R Rpl7ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) complement(join(364338..364967,365436..365529,365989..365999)) Saccharomyces cerevisiae 852804 NP_011440.1 CDS MPS2 NC_001139.8 366928 368091 R Mps2p; Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p complement(366928..368091) Saccharomyces cerevisiae 852805 NP_011442.1 CDS HSF1 NC_001139.8 368756 371257 D Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated; Hsf1p 368756..371257 Saccharomyces cerevisiae 852806 NP_011444.1 CDS AFT1 NC_001139.8 372015 374087 D Transcription factor involved in iron utilization and homeostasis; binds the consensus site PyPuCACCCPu and activates the expression of target genes in response to changes in iron availability; Aft1p 372015..374087 Saccharomyces cerevisiae 852809 NP_011445.1 CDS RPB9 NC_001139.8 374462 374830 R Rpb9p; RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site; involved in telomere maintenance complement(374462..374830) Saccharomyces cerevisiae 852810 NP_011447.1 CDS MNP1 NC_001139.8 375090 375674 D Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth; Mnp1p 375090..375674 Saccharomyces cerevisiae 852811 NP_011448.1 CDS NPY1 NC_001139.8 376104 377258 D Npy1p; NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes 376104..377258 Saccharomyces cerevisiae 852813 NP_011449.1 CDS SGF73 NC_001139.8 377612 379585 D Sgf73p; 73 kDa subunit of SAGA histone acetyltransferase complex; involved in formation of the preinitiation complex assembly at promoters 377612..379585 Saccharomyces cerevisiae 852814 NP_011450.1 CDS ALG2 NC_001139.8 379763 381274 R Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; Alg2p complement(379763..381274) Saccharomyces cerevisiae 852815 NP_011451.1 CDS MRH4 NC_001139.8 381526 383211 R Mrh4p; Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function complement(381526..383211) Saccharomyces cerevisiae 852816 NP_011452.1 CDS PUS2 NC_001139.8 383657 384769 D Pus2p; Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification 383657..384769 Saccharomyces cerevisiae 852817 NP_011453.1 CDS PYC1 NC_001139.8 385199 388735 D Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc2p but differentially regulated; mutations in the human homolog are associated with lactic acidosis; Pyc1p 385199..388735 Saccharomyces cerevisiae 852818 NP_011454.1 CDS DUO1 NC_001139.8 388971 389714 R Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Duo1p complement(388971..389714) Saccharomyces cerevisiae 852819 NP_011455.1 CDS YBP2 NC_001139.8 390070 391995 D Ybp2p; Protein with a role in resistance to oxidative stress; has similarity to Ybp1p, which is involved in regulation of the transcription factor Yap1p via oxidation of specific cysteine residues 390070..391995 Saccharomyces cerevisiae 852820 NP_011456.2 CDS YGL059W NC_001139.8 392228 393703 D Ygl059wp; Protein kinase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 392228..393703 Saccharomyces cerevisiae 852821 NP_011457.1 CDS RAD6 NC_001139.8 393992 394510 D Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p); Rad6p 393992..394510 Saccharomyces cerevisiae 852822 NP_011458.1 CDS YGL057C NC_001139.8 394972 395835 R Ygl057cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant exhibits a growth defect on a non-fermentable (respiratory) carbon source complement(394972..395835) Saccharomyces cerevisiae 852823 NP_011459.1 CDS SDS23 NC_001139.8 396041 397624 R Sds23p; One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation complement(396041..397624) Saccharomyces cerevisiae 852824 NP_011460.1 CDS OLE1 NC_001139.8 398631 400163 D Ole1p; Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria 398631..400163 Saccharomyces cerevisiae 852825 NP_011461.1 CDS ERV14 NC_001139.8 400874 401290 R Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; Erv14p complement(400874..401290) Saccharomyces cerevisiae 852826 NP_011462.1 CDS PRM8 NC_001139.8 402592 403305 D Prm8p; Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family 402592..403305 Saccharomyces cerevisiae 852828 NP_011464.1 CDS MST27 NC_001139.8 403690 404394 D Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles; Mst27p 403690..404394 Saccharomyces cerevisiae 852830 NP_011465.1 CDS TYW3 NC_001139.8 405779 406600 D Tyw3p; Protein required for the synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions 405779..406600 Saccharomyces cerevisiae 852832 NP_011466.1 CDS TIF4632 NC_001139.8 406863 409607 R Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p; Tif4632p complement(406863..409607) Saccharomyces cerevisiae 852833 NP_011467.1 CDS RPT6 NC_001139.8 410072 411289 R Rpt6p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle complement(410072..411289) Saccharomyces cerevisiae 852834 NP_011468.1 CDS ALG13 NC_001139.8 411555 412163 D Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; Alg13p 411555..412163 Saccharomyces cerevisiae 852835 NP_011470.2 CDS RIM8 NC_001139.8 414106 415734 D Rim8p; Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalF 414106..415734 Saccharomyces cerevisiae 852837 NP_011471.1 CDS RNA15 NC_001139.8 416150 417040 R Rna15p; Cleavage and polyadenylation factor I (CF I) component involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p complement(416150..417040) Saccharomyces cerevisiae 852838 NP_011472.1 CDS DST1 NC_001139.8 417487 418416 D General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; Dst1p 417487..418416 Saccharomyces cerevisiae 852839 NP_878074.1 CDS YGL041C-B NC_001139.8 418709 418891 R Ygl041c-bp; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(418709..418891) Saccharomyces cerevisiae 1466454 NP_001106172.1 CDS YGL041W-A NC_001139.8 419047 419295 D Ygl041w-ap; Putative protein of unknown function, conserved in fungi; identified by expression profiling and mass spectrometry 419047..419295 Saccharomyces cerevisiae 5814844 NP_011475.1 CDS HEM2 NC_001139.8 419533 420561 R Hem2p; Delta-aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of delta-aminolevulinic acid to porphobilinogen, the second step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus complement(419533..420561) Saccharomyces cerevisiae 852842 NP_011476.1 CDS YGL039W NC_001139.8 423967 425013 D Ygl039wp; Oxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) 423967..425013 Saccharomyces cerevisiae 852844 NP_011477.1 CDS OCH1 NC_001139.8 425368 426810 R Och1p; Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins complement(425368..426810) Saccharomyces cerevisiae 852845 NP_011478.1 CDS PNC1 NC_001139.8 427303 427953 R Pnc1p; Nicotinamidase that converts nicotinamide to nicotinic acid as part of the NAD(+) salvage pathway, required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span complement(427303..427953) Saccharomyces cerevisiae 852846 NP_011479.1 CDS YGL036W NC_001139.8 428606 431335 D Ygl036wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene 428606..431335 Saccharomyces cerevisiae 852847 NP_011480.1 CDS MIG1 NC_001139.8 431553 433067 R Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; Mig1p complement(431553..433067) Saccharomyces cerevisiae 852848 NP_011482.2 CDS HOP2 NC_001139.8 435630 436356 D Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair; Hop2p join(435630..435684,435755..436356) Saccharomyces cerevisiae 852850 NP_011483.1 CDS AGA2 NC_001139.8 436575 436838 R Aga2p; Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds complement(436575..436838) Saccharomyces cerevisiae 852851 NP_011484.1 CDS RPL24A NC_001139.8 437472 437939 R Rpl24ap; Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate complement(437472..437939) Saccharomyces cerevisiae 852852 NP_011485.1 CDS RPL30 NC_001139.8 439096 439643 D Rpl30p; Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript join(439096..439098,439329..439643) Saccharomyces cerevisiae 852853 NP_011486.1 CDS CGR1 NC_001139.8 440068 440430 D Protein involved in nucleolar integrity and processing of the pre-rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; Cgr1p 440068..440430 Saccharomyces cerevisiae 852854 NP_011487.1 CDS SCW11 NC_001139.8 441286 442914 R Scw11p; Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p complement(441286..442914) Saccharomyces cerevisiae 852856 NP_011488.1 CDS CWH41 NC_001139.8 443647 446148 R Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress; Cwh41p complement(443647..446148) Saccharomyces cerevisiae 852857 NP_011489.1 CDS TRP5 NC_001139.8 446417 448540 R Tryptophan synthase involved in tryptophan biosynthesis, regulated by the general control system of amino acid biosynthesis; Trp5p complement(446417..448540) Saccharomyces cerevisiae 852858 NP_011490.2 CDS PGD1 NC_001139.8 448769 449962 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor; Pgd1p complement(448769..449962) Saccharomyces cerevisiae 852860 NP_011492.1 CDS PIB2 NC_001139.8 450202 452109 R Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene expression; similar to Fab1 and Vps27; Pib2p complement(450202..452109) Saccharomyces cerevisiae 852861 NP_011493.1 CDS STT3 NC_001139.8 452409 454565 D Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis; Stt3p 452409..454565 Saccharomyces cerevisiae 852862 NP_011494.1 CDS ALK1 NC_001139.8 454790 457072 D Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk2p and to mammalian haspins; Alk1p 454790..457072 Saccharomyces cerevisiae 852863 NP_011495.1 CDS GET1 NC_001139.8 457168 457875 R Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance; Get1p complement(457168..457875) Saccharomyces cerevisiae 852864 NP_011496.1 CDS CKB1 NC_001139.8 458161 458997 D Ckb1p; Beta regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB2, the many substrates include transcription factors and all RNA polymerases 458161..458997 Saccharomyces cerevisiae 852865 NP_011497.1 CDS JAC1 NC_001139.8 459115 459669 R Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix; Jac1p complement(459115..459669) Saccharomyces cerevisiae 852866 NP_011498.1 CDS ATE1 NC_001139.8 459859 461370 D Ate1p; Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway 459859..461370 Saccharomyces cerevisiae 852867 NP_011499.1 CDS KAP122 NC_001139.8 461671 464916 D Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance; Kap122p 461671..464916 Saccharomyces cerevisiae 852868 NP_011500.1 CDS YGL015C NC_001139.8 465048 465440 R Ygl015cp complement(465048..465440) Saccharomyces cerevisiae 852869 NP_011501.1 CDS PUF4 NC_001139.8 466146 468812 D Puf4p; Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors 466146..468812 Saccharomyces cerevisiae 852870 NP_011502.1 CDS PDR1 NC_001139.8 469097 472303 R Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; Pdr1p complement(469097..472303) Saccharomyces cerevisiae 852871 NP_011503.1 CDS ERG4 NC_001139.8 472860 474281 D Erg4p; C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol 472860..474281 Saccharomyces cerevisiae 852872 NP_011504.1 CDS SCL1 NC_001139.8 474494 475252 R Scl1p; Alpha subunit of the 20S core complex of the 26S proteasome involved in the degradation of ubiquitinated substrates; essential for growth; detected in the mitochondria complement(474494..475252) Saccharomyces cerevisiae 852873 NP_011505.1 CDS YGL010W NC_001139.8 475550 476074 D Ygl010wp; Putative protein of unknown function; YGL010W is not an essential gene 475550..476074 Saccharomyces cerevisiae 852874 NP_011506.1 CDS LEU1 NC_001139.8 476318 478657 R Leu1p; Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway complement(476318..478657) Saccharomyces cerevisiae 852875 NP_011507.1 CDS PMA1 NC_001139.8 479915 482671 R Pma1p; Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases complement(479915..482671) Saccharomyces cerevisiae 852876 NP_878075.1 CDS YGL007C-A NC_001139.8 482946 483032 R Ygl007c-ap; Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; haploid deletion strain exhibits a slow-growth phenotype complement(482946..483032) Saccharomyces cerevisiae 1466455 NP_878076.1 CDS YGL006W-A NC_001139.8 485428 485538 D Ygl006w-ap; Putative protein of unknown function; identified by SAGE 485428..485538 Saccharomyces cerevisiae 1466456 NP_011509.1 CDS PMC1 NC_001139.8 485925 489446 D Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a; Pmc1p 485925..489446 Saccharomyces cerevisiae 852878 NP_011510.1 CDS COG7 NC_001139.8 489712 490551 R Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog7p complement(489712..490551) Saccharomyces cerevisiae 852879 NP_011511.1 CDS RPN14 NC_001139.8 490707 491960 R Rpn14p; Putative non-ATPase subunit of the 19S regulatory particle of the 26S proteasome; localized to the cytoplasm complement(490707..491960) Saccharomyces cerevisiae 852880 NP_011512.1 CDS CDH1 NC_001139.8 492478 494178 R Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including CDC20, ASE1, CIN8 and FIN1; Cdh1p complement(492478..494178) Saccharomyces cerevisiae 852881 NP_011513.1 CDS ERP6 NC_001139.8 494521 495171 D Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Erp6p 494521..495171 Saccharomyces cerevisiae 852882 NP_011514.1 CDS ERG26 NC_001139.8 495457 496506 R Erg26p; C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis complement(495457..496506) Saccharomyces cerevisiae 852883 NP_011515.2 CDS AML1 NC_001139.8 497137 498038 R Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae; Aml1p complement(join(497137..497369,497463..497941,498004..498038)) Saccharomyces cerevisiae 852884 NP_011516.1 CDS SWC4 NC_001139.8 498480 499910 R Swc4p; Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex complement(498480..499910) Saccharomyces cerevisiae 852885 NP_011517.1 CDS CUL3 NC_001139.8 500136 502370 D Ubiquitin-protein ligase, member of the cullin family with similarity to Cdc53p and human CUL3; required for ubiquitin-dependent degredation of the RNA Polymerase II subunit RPO21; Cul3p 500136..502370 Saccharomyces cerevisiae 852886 NP_011518.1 CDS PEX31 NC_001139.8 502942 504330 D Pex31p; Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p 502942..504330 Saccharomyces cerevisiae 852887 NP_011519.1 CDS TFG2 NC_001139.8 504661 505863 R Tfg2p; TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 complement(504661..505863) Saccharomyces cerevisiae 852888 NP_011520.2 CDS PRP18 NC_001139.8 506074 506829 D Splicing factor involved in the positioning of the 3' splice site during the second catalytic step of splicing, part of snRNP U5, interacts with Slu7p; Prp18p 506074..506829 Saccharomyces cerevisiae 852889 NP_011521.1 CDS MUQ1 NC_001139.8 506973 507944 D Muq1p; Choline phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases 506973..507944 Saccharomyces cerevisiae 852890 NP_011522.1 CDS STF2 NC_001139.8 508114 508368 R Stf2p; Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein complement(508114..508368) Saccharomyces cerevisiae 852891 NP_011523.1 CDS SEC9 NC_001139.8 509051 511006 R t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog; Sec9p complement(509051..511006) Saccharomyces cerevisiae 852892 NP_011524.1 CDS NMA2 NC_001139.8 511549 512736 D Nma2p; Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway 511549..512736 Saccharomyces cerevisiae 852893 NP_011526.1 CDS YGR012W NC_001139.8 513163 514344 D Ygr012wp; Putative cysteine synthase, localized to the mitochondrial outer membrane 513163..514344 Saccharomyces cerevisiae 852895 NP_011527.1 CDS SNU71 NC_001139.8 514558 516420 D Snu71p; Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart 514558..516420 Saccharomyces cerevisiae 852896 NP_011528.1 CDS MSB2 NC_001139.8 516947 520867 D Mucin family member involved in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate; Msb2p 516947..520867 Saccharomyces cerevisiae 852897 NP_011529.1 CDS YGR015C NC_001139.8 521094 522080 R Ygr015cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion complement(521094..522080) Saccharomyces cerevisiae 852898 NP_011530.1 CDS YGR016W NC_001139.8 522265 522837 D Ygr016wp; Putative protein of unknown function 522265..522837 Saccharomyces cerevisiae 852899 NP_011531.1 CDS YGR017W NC_001139.8 523791 524684 D Ygr017wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm 523791..524684 Saccharomyces cerevisiae 852900 NP_011533.1 CDS UGA1 NC_001139.8 525233 526648 D Uga1p; Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization 525233..526648 Saccharomyces cerevisiae 852902 NP_011534.1 CDS VMA7 NC_001139.8 526977 527333 R Vma7p; Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane complement(526977..527333) Saccharomyces cerevisiae 852903 NP_011535.1 CDS YGR021W NC_001139.8 527636 528508 D Ygr021wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 527636..528508 Saccharomyces cerevisiae 852904 NP_011537.1 CDS MTL1 NC_001139.8 529268 530923 D Protein with both structural and functional similarity to Mid2p, which is a plasma membrane sensor required for cell integrity signaling during pheromone-induced morphogenesis; suppresses rgd1 null mutations; Mtl1p 529268..530923 Saccharomyces cerevisiae 852905 NP_011538.1 CDS THG1 NC_001139.8 531887 532600 R tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes; Thg1p complement(531887..532600) Saccharomyces cerevisiae 852908 NP_011540.1 CDS YGR026W NC_001139.8 532989 533825 D Ygr026wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery 532989..533825 Saccharomyces cerevisiae 852910 NP_011541.1 CDS RPS25A NC_001139.8 534136 534462 R Rps25ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein complement(534136..534462) Saccharomyces cerevisiae 852911 NP_058158.1 CDS YGR027W-B NC_001139.8 536061 541329 D Ygr027w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(536061..537365,537367..541329) Saccharomyces cerevisiae 852913 NP_058159.1 CDS YGR027W-A NC_001139.8 536061 537383 D Ygr027w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 536061..537383 Saccharomyces cerevisiae 852912 NP_011542.1 CDS MSP1 NC_001139.8 542207 543295 D Msp1p; Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase 542207..543295 Saccharomyces cerevisiae 852915 NP_011543.2 CDS ERV1 NC_001139.8 543557 544209 D Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR); Erv1p join(543557..543642,543726..544209) Saccharomyces cerevisiae 852916 NP_011544.1 CDS POP6 NC_001139.8 545685 546161 R Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Pop6p complement(545685..546161) Saccharomyces cerevisiae 852918 NP_011545.1 CDS YGR031W NC_001139.8 546445 547473 D Ygr031wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 546445..547473 Saccharomyces cerevisiae 852919 NP_011546.1 CDS GSC2 NC_001139.8 548268 553955 D Gsc2p; Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) 548268..553955 Saccharomyces cerevisiae 852920 NP_011547.1 CDS TIM21 NC_001139.8 554252 554971 R Tim21p; Constituent of the mitochondrial inner membrane presequence translocase (TIM23 complex); may regulate protein import by binding to both the translocase of the outer membrane (TOM) and presequence-associated motor (PAM) complexes complement(554252..554971) Saccharomyces cerevisiae 852921 NP_011548.2 CDS RPL26B NC_001139.8 555816 556676 D Rpl26bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Ap and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA join(555816..555834,556312..556676) Saccharomyces cerevisiae 852922 NP_011549.1 CDS YGR035C NC_001139.8 557076 557426 R Ygr035cp; Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance complement(557076..557426) Saccharomyces cerevisiae 852923 NP_878077.1 CDS YGR035W-A NC_001139.8 557569 557790 D Ygr035w-ap; Putative protein of unknown function 557569..557790 Saccharomyces cerevisiae 1466457 NP_011550.1 CDS CAX4 NC_001139.8 558153 558872 R Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation; Cax4p complement(558153..558872) Saccharomyces cerevisiae 852924 NP_011551.1 CDS ACB1 NC_001139.8 559735 559998 R Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; Acb1p complement(559735..559998) Saccharomyces cerevisiae 852925 NP_011552.1 CDS ORM1 NC_001139.8 560686 561354 D Orm1p; Evolutionarily conserved protein with similarity to Orm2p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum 560686..561354 Saccharomyces cerevisiae 852926 NP_058160.1 CDS YGR038C-B NC_001139.8 562203 567471 R Ygr038c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(562203..566165,566167..567471)) Saccharomyces cerevisiae 852928 NP_058161.1 CDS YGR038C-A NC_001139.8 566149 567471 R Ygr038c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(566149..567471) Saccharomyces cerevisiae 852929 NP_011554.1 CDS KSS1 NC_001139.8 575402 576508 D Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains; Kss1p 575402..576508 Saccharomyces cerevisiae 852931 NP_011555.1 CDS BUD9 NC_001139.8 577491 579134 D Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; Bud9p 577491..579134 Saccharomyces cerevisiae 852932 NP_011556.1 CDS YGR042W NC_001139.8 579480 580295 D Ygr042wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 579480..580295 Saccharomyces cerevisiae 852933 NP_011557.1 CDS NQM1 NC_001139.8 580439 581440 R Protein of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; null mutant non-quiescent cells exhibit reduced reproductive capacity; Nqm1p complement(580439..581440) Saccharomyces cerevisiae 852934 NP_011558.1 CDS RME1 NC_001139.8 582994 583896 R Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-a2 regulator; mediates cell type control of sporulation; Rme1p complement(582994..583896) Saccharomyces cerevisiae 852935 NP_011560.1 CDS TAM41 NC_001139.8 584899 586056 D Tam41p; Mitochondrial protein involved in protein import into the mitochondrial matrix; maintains the functional integrity of the TIM23 protein translocator complex; viability of null mutant is strain-dependent; mRNA is targeted to the bud 584899..586056 Saccharomyces cerevisiae 852937 NP_011561.1 CDS TFC4 NC_001139.8 586396 589473 R Tfc4p; One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 complement(586396..589473) Saccharomyces cerevisiae 852938 NP_011562.1 CDS UFD1 NC_001139.8 589830 590915 D Ufd1p; Protein that interacts with Cdc48p and Npl4p, involved in recognition of polyubiquitinated proteins and their presentation to the 26S proteasome for degradation; involved in transporting proteins from the ER to the cytosol 589830..590915 Saccharomyces cerevisiae 852939 NP_011563.1 CDS SCM4 NC_001139.8 591318 591881 D Scm4p; Potential regulatory effector of CDC4 function, suppresses a temperature-sensitive allele of CDC4, tripartite protein structure in which a charged region separates two uncharged domains, not essential for mitosis or meiosis 591318..591881 Saccharomyces cerevisiae 852940 NP_011566.1 CDS FMP48 NC_001139.8 593600 594709 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp48p 593600..594709 Saccharomyces cerevisiae 852943 NP_011567.1 CDS YGR053C NC_001139.8 594990 595841 R Ygr053cp; Putative protein of unknown function complement(594990..595841) Saccharomyces cerevisiae 852944 NP_011568.1 CDS YGR054W NC_001139.8 596697 598625 D Ygr054wp; Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A 596697..598625 Saccharomyces cerevisiae 852945 NP_011569.1 CDS MUP1 NC_001139.8 599421 601145 D Mup1p; High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake 599421..601145 Saccharomyces cerevisiae 852946 NP_011570.1 CDS RSC1 NC_001139.8 601665 604451 D Rsc1p; Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook 601665..604451 Saccharomyces cerevisiae 852947 NP_011571.2 CDS LST7 NC_001139.8 604777 605505 R Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface; Lst7p complement(604777..605505) Saccharomyces cerevisiae 852948 NP_011572.1 CDS PEF1 NC_001139.8 606140 607147 D Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; Pef1p 606140..607147 Saccharomyces cerevisiae 852949 NP_011573.1 CDS SPR3 NC_001139.8 607567 609105 D Spr3p; Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI 607567..609105 Saccharomyces cerevisiae 852950 NP_011574.1 CDS ERG25 NC_001139.8 610568 611497 D Erg25p; C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol 610568..611497 Saccharomyces cerevisiae 852951 NP_011575.1 CDS ADE6 NC_001139.8 611893 615969 R Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; Ade6p complement(611893..615969) Saccharomyces cerevisiae 852952 NP_011576.1 CDS COX18 NC_001139.8 616332 617282 R Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; Cox18p complement(616332..617282) Saccharomyces cerevisiae 852953 NP_011577.1 CDS SPT4 NC_001139.8 617520 617828 R Spt4p; Protein that forms a complex with Spt5p and mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing; in addition, Spt4p is involved in kinetochore function and gene silencing complement(617520..617828) Saccharomyces cerevisiae 852955 NP_011579.1 CDS VHT1 NC_001139.8 618081 619862 R Vht1p; High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin complement(618081..619862) Saccharomyces cerevisiae 852956 NP_011580.1 CDS YGR066C NC_001139.8 620925 621803 R Ygr066cp; Putative protein of unknown function complement(620925..621803) Saccharomyces cerevisiae 852957 NP_011581.1 CDS YGR067C NC_001139.8 622406 624790 R Ygr067cp; Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p complement(622406..624790) Saccharomyces cerevisiae 852958 NP_011582.1 CDS YGR068C NC_001139.8 625328 627088 R Ygr068cp; Putative protein of unknown function; YGR068C is not an essential gene. complement(625328..627088) Saccharomyces cerevisiae 852960 NP_011584.1 CDS ROM1 NC_001139.8 627810 631277 D Rom1p; GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP 627810..631277 Saccharomyces cerevisiae 852961 NP_011585.1 CDS YGR071C NC_001139.8 631485 634067 R Ygr071cp; Putative protein of unknown function; deletion mutant has increased glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the nucleus complement(631485..634067) Saccharomyces cerevisiae 852962 NP_011586.1 CDS UPF3 NC_001139.8 634308 635471 D Upf3p; Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance 634308..635471 Saccharomyces cerevisiae 852963 NP_011588.1 CDS SMD1 NC_001139.8 635716 636156 D Smd1p; Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1 635716..636156 Saccharomyces cerevisiae 852964 NP_011589.1 CDS PRP38 NC_001139.8 636151 636879 R Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly; Prp38p complement(636151..636879) Saccharomyces cerevisiae 852966 NP_011590.1 CDS MRPL25 NC_001139.8 637108 637581 R Mrpl25p; Mitochondrial ribosomal protein of the large subunit complement(637108..637581) Saccharomyces cerevisiae 852967 NP_011591.1 CDS PEX8 NC_001139.8 637748 639517 R Pex8p; Intraperoxisomal organizer of the peroxisomal import machinery, tightly associated with the lumenal face of the peroxisomal membrane, essential for peroxisome biogenesis, binds PTS1-signal receptor Pex5p complement(637748..639517) Saccharomyces cerevisiae 852968 NP_011592.1 CDS PAC10 NC_001139.8 639776 640375 R Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding; Pac10p complement(639776..640375) Saccharomyces cerevisiae 852969 NP_011593.1 CDS YGR079W NC_001139.8 640724 641836 D Ygr079wp; Putative protein of unknown function; YGR079W is not an essential gene 640724..641836 Saccharomyces cerevisiae 852970 NP_011594.1 CDS TWF1 NC_001139.8 642014 643012 D Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly; Twf1p 642014..643012 Saccharomyces cerevisiae 852971 NP_011595.1 CDS SLX9 NC_001139.8 643152 643784 R Slx9p; Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant complement(643152..643784) Saccharomyces cerevisiae 852972 NP_011596.1 CDS TOM20 NC_001139.8 644048 644599 D Tom20p; Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins 644048..644599 Saccharomyces cerevisiae 852973 NP_011597.1 CDS GCD2 NC_001139.8 644864 646819 R Gcd2p; Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression complement(644864..646819) Saccharomyces cerevisiae 852974 NP_011598.1 CDS MRP13 NC_001139.8 647127 648146 R Mrp13p; Mitochondrial ribosomal protein of the small subunit complement(647127..648146) Saccharomyces cerevisiae 852975 NP_011599.1 CDS RPL11B NC_001139.8 648387 648911 R Rpl11bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11 complement(648387..648911) Saccharomyces cerevisiae 852976 NP_011600.1 CDS PIL1 NC_001139.8 649602 650621 R Primary component of eisosomes, which are large immobile cell cortex structures associated with endocytosis; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; Pil1p complement(649602..650621) Saccharomyces cerevisiae 852977 NP_011601.1 CDS PDC6 NC_001139.8 651294 652985 R Pdc6p; Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation complement(651294..652985) Saccharomyces cerevisiae 852978 NP_011602.2 CDS CTT1 NC_001139.8 654638 656326 D Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide; Ctt1p 654638..656326 Saccharomyces cerevisiae 852979 NP_011603.1 CDS NNF2 NC_001139.8 656964 659774 D Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation; Nnf2p 656964..659774 Saccharomyces cerevisiae 852980 NP_011604.1 CDS UTP22 NC_001139.8 662362 666075 D Utp22p; Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data 662362..666075 Saccharomyces cerevisiae 852982 NP_011605.1 CDS PRP31 NC_001139.8 666345 667829 D Splicing factor, component of the U4/U6-U5 snRNP complex; Prp31p 666345..667829 Saccharomyces cerevisiae 852983 NP_011606.1 CDS DBF2 NC_001139.8 668193 669911 D Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; Dbf2p 668193..669911 Saccharomyces cerevisiae 852984 NP_011607.1 CDS DRN1 NC_001139.8 670392 671915 D Putative debranching enzyme associated ribonuclease; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; Drn1p 670392..671915 Saccharomyces cerevisiae 852985 NP_011608.1 CDS VAS1 NC_001139.8 672190 675504 D Vas1p; Mitochondrial and cytoplasmic valyl-tRNA synthetase 672190..675504 Saccharomyces cerevisiae 852986 NP_011609.2 CDS RRP46 NC_001139.8 675675 676346 R Rrp46p; Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex complement(675675..676346) Saccharomyces cerevisiae 852987 NP_011610.1 CDS TPC1 NC_001139.8 676625 677569 D Tpc1p; Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family 676625..677569 Saccharomyces cerevisiae 852988 NP_011611.1 CDS ASK10 NC_001139.8 678699 682139 D Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p, which relieves repression of stress-response genes; Ask10p 678699..682139 Saccharomyces cerevisiae 852989 NP_011612.1 CDS ESP1 NC_001139.8 682570 687462 R Separase, a caspase-like cysteine protease that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; Esp1p complement(682570..687462) Saccharomyces cerevisiae 852990 NP_011613.1 CDS TEL2 NC_001139.8 687903 689969 D Tel2p; Essential DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect 687903..689969 Saccharomyces cerevisiae 852991 NP_011614.1 CDS MDR1 NC_001139.8 690249 693101 D Mdr1p; Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function 690249..693101 Saccharomyces cerevisiae 852992 NP_011615.1 CDS PCP1 NC_001139.8 693367 694407 D Pcp1p; Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases 693367..694407 Saccharomyces cerevisiae 852993 NP_011616.1 CDS YGR102C NC_001139.8 694588 695139 R Ygr102cp; Putative protein of unknown function; transposon insertion mutant is salt sensitive and deletion mutant has growth defects; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(694588..695139) Saccharomyces cerevisiae 852994 NP_011617.1 CDS NOP7 NC_001139.8 695421 697238 D Nucleolar protein involved in rRNA processing and 60S ribosomal subunit biogenesis; constituent of several different pre-ribosomal particles; required for exit from G0 and the initiation of cell proliferation; Nop7p 695421..697238 Saccharomyces cerevisiae 852995 NP_011618.1 CDS SRB5 NC_001139.8 697449 698372 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in telomere maintenance; Srb5p complement(697449..698372) Saccharomyces cerevisiae 852996 NP_011619.1 CDS VMA21 NC_001139.8 698603 698836 D Vma21p; Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) 698603..698836 Saccharomyces cerevisiae 852997 NP_011620.1 CDS YGR106C NC_001139.8 698993 699790 R Ygr106cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar memebrane complement(698993..699790) Saccharomyces cerevisiae 852998 NP_011622.1 CDS CLB1 NC_001139.8 703640 705055 D Clb1p; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome 703640..705055 Saccharomyces cerevisiae 853002 NP_011623.1 CDS CLB6 NC_001139.8 705363 706505 R Clb6p; B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 complement(705363..706505) Saccharomyces cerevisiae 853003 NP_011624.1 CDS YGR109W-B NC_001139.8 707614 712258 D Ygr109w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(707614..708463,708465..712258) Saccharomyces cerevisiae 853006 NP_058162.1 CDS YGR109W-A NC_001139.8 707614 708486 D Ygr109w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 707614..708486 Saccharomyces cerevisiae 853005 NP_011625.1 CDS YGR110W NC_001139.8 713713 715050 D Ygr110wp; Putative protein of unknown function; transcription is increased in response to genotoxic stress 713713..715050 Saccharomyces cerevisiae 853007 NP_011626.1 CDS YGR111W NC_001139.8 715832 717034 D Ygr111wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 715832..717034 Saccharomyces cerevisiae 853008 NP_011627.1 CDS SHY1 NC_001139.8 717362 718531 D Shy1p; Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome 717362..718531 Saccharomyces cerevisiae 853009 NP_011628.2 CDS DAM1 NC_001139.8 718897 719928 D Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments; Dam1p 718897..719928 Saccharomyces cerevisiae 853010 NP_011631.1 CDS SPT6 NC_001139.8 720413 724768 D Spt6p; Essential protein that interacts with histones and is involved in nucleosome disassembly and reassembly during transcription elongation 720413..724768 Saccharomyces cerevisiae 853011 NP_011632.1 CDS YGR117C NC_001139.8 725053 726483 R Ygr117cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(725053..726483) Saccharomyces cerevisiae 853014 NP_011633.1 CDS RPS23A NC_001139.8 726978 727735 D Rps23ap; Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Bp and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal join(726978..727042,727363..727735) Saccharomyces cerevisiae 853015 NP_011634.1 CDS NUP57 NC_001139.8 728051 729676 R Nup57p; Nucleoporin, essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p complement(728051..729676) Saccharomyces cerevisiae 853016 NP_011635.2 CDS COG2 NC_001139.8 730038 730826 R Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog2p complement(730038..730826) Saccharomyces cerevisiae 853017 NP_011636.1 CDS MEP1 NC_001139.8 731454 732932 R Mep1p; Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation complement(731454..732932) Saccharomyces cerevisiae 853019 NP_878078.1 CDS YGR121W-A NC_001139.8 733417 733632 D Ygr121w-ap; Putative protein of unknown function 733417..733632 Saccharomyces cerevisiae 1466458 NP_011637.1 CDS YGR122W NC_001139.8 733940 735148 D Ygr122wp; Putative protein of unknown function; deletion mutants do not properly process Rim101p and have decreased resistance to rapamycin; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 733940..735148 Saccharomyces cerevisiae 853020 NP_011639.1 CDS PPT1 NC_001139.8 736667 738208 R Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth; computational analyses suggest roles in phosphate metabolism and rRNA processing; Ppt1p complement(736667..738208) Saccharomyces cerevisiae 853023 NP_011640.1 CDS ASN2 NC_001139.8 739949 741667 D Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn2p 739949..741667 Saccharomyces cerevisiae 853025 NP_011641.1 CDS YGR125W NC_001139.8 742330 745440 D Ygr125wp; Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 742330..745440 Saccharomyces cerevisiae 853026 NP_011642.1 CDS YGR126W NC_001139.8 745840 746532 D Ygr126wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS 745840..746532 Saccharomyces cerevisiae 853027 NP_011643.1 CDS YGR127W NC_001139.8 746803 747741 D Ygr127wp; Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response 746803..747741 Saccharomyces cerevisiae 853028 NP_011644.1 CDS UTP8 NC_001139.8 747955 750096 R Utp8p; Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(747955..750096) Saccharomyces cerevisiae 853029 NP_011645.1 CDS SYF2 NC_001139.8 750405 751052 D Syf2p; Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression 750405..751052 Saccharomyces cerevisiae 853030 NP_011646.1 CDS YGR130C NC_001139.8 751399 753849 R Ygr130cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) complement(751399..753849) Saccharomyces cerevisiae 853031 NP_011647.1 CDS YGR131W NC_001139.8 754731 755255 D Ygr131wp; Protein of unknown function; expression induced in response to ketoconazole; promoter region contains a sterol regulatory element motif, which has been identified as a Upc2p-binding site 754731..755255 Saccharomyces cerevisiae 853032 NP_011648.1 CDS PHB1 NC_001139.8 755594 756457 R Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; Phb1p complement(755594..756457) Saccharomyces cerevisiae 853033 NP_011649.1 CDS PEX4 NC_001139.8 756901 757452 D Pex4p; Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis 756901..757452 Saccharomyces cerevisiae 853034 NP_011650.1 CDS CAF130 NC_001139.8 757775 761143 D Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation; Caf130p 757775..761143 Saccharomyces cerevisiae 853035 NP_011651.1 CDS PRE9 NC_001139.8 761397 762173 D Pre9p; 20S proteasome beta-type subunit; the only nonessential 20S subunit 761397..762173 Saccharomyces cerevisiae 853036 NP_011652.1 CDS LSB1 NC_001139.8 762433 763158 D Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; Lsb1p 762433..763158 Saccharomyces cerevisiae 853037 NP_011654.1 CDS TPO2 NC_001139.8 763767 765611 R Tpo2p; Polyamine transport protein specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; member of the major facilitator superfamily complement(763767..765611) Saccharomyces cerevisiae 853039 NP_011656.1 CDS CBF2 NC_001139.8 767434 770304 D Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; Cbf2p 767434..770304 Saccharomyces cerevisiae 853041 NP_011657.1 CDS VPS62 NC_001139.8 770574 771977 D Vps62p; Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins 770574..771977 Saccharomyces cerevisiae 853042 NP_011658.1 CDS BTN2 NC_001139.8 772459 773691 D v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; Btn2p 772459..773691 Saccharomyces cerevisiae 853043 NP_011659.1 CDS SKN1 NC_001139.8 775198 777513 D Skn1p; Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p 775198..777513 Saccharomyces cerevisiae 853045 NP_011660.1 CDS THI4 NC_001139.8 780404 781384 D Thiazole synthase, catalyzes formation of the thiazole moiety of thiamin pyrophosphate; required for thiamine biosynthesis and for mitochondrial genome stability; Thi4p 780404..781384 Saccharomyces cerevisiae 853047 NP_011661.1 CDS ENP2 NC_001139.8 781772 783895 D Essential nucleolar protein of unknown function; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p; Enp2p 781772..783895 Saccharomyces cerevisiae 853048 NP_011662.1 CDS YGR146C NC_001139.8 784228 784863 R Ygr146cp; Putative protein of unknown function; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of a temperature sensitive hsf1 mutant complement(784228..784863) Saccharomyces cerevisiae 853049 NP_878079.1 CDS YGR146C-A NC_001139.8 785281 785442 R Ygr146c-ap; Putative protein of unknown function complement(785281..785442) Saccharomyces cerevisiae 1466459 NP_011663.1 CDS NAT2 NC_001139.8 786059 786925 R Nat2p; N alpha-acetyl-transferase, transfers acetyl group from acetyl coenzyme A to the N-terminal methionine residues of proteins complement(786059..786925) Saccharomyces cerevisiae 853050 NP_011664.1 CDS RPL24B NC_001139.8 787317 787784 R Rpl24bp; Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate complement(787317..787784) Saccharomyces cerevisiae 853051 NP_011665.1 CDS YGR149W NC_001139.8 789036 790334 D Ygr149wp; Putative protein of unknown function; predicted to be an integal membrane protein 789036..790334 Saccharomyces cerevisiae 853052 NP_011666.1 CDS DMR1 NC_001139.8 790464 793058 R Protein of unknown function, contains PPR motifs; mutant has growth defects on both non-fermentable carbon sources and rich medium; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Dmr1p complement(790464..793058) Saccharomyces cerevisiae 853053 NP_011668.1 CDS RSR1 NC_001139.8 794679 795497 R Rsr1p; GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases complement(794679..795497) Saccharomyces cerevisiae 853056 NP_011669.1 CDS YGR153W NC_001139.8 796097 796750 D Ygr153wp; Putative protein of unknown function 796097..796750 Saccharomyces cerevisiae 853057 NP_011670.1 CDS GTO1 NC_001139.8 796803 797873 R Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization; Gto1p complement(796803..797873) Saccharomyces cerevisiae 853058 NP_011671.1 CDS CYS4 NC_001139.8 798548 800071 D Cystathionine beta-synthase, catalyzes the synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; Cys4p 798548..800071 Saccharomyces cerevisiae 853059 NP_011672.1 CDS PTI1 NC_001139.8 800551 801828 D Pti1p; Pta1p Interacting protein 800551..801828 Saccharomyces cerevisiae 853060 NP_011673.1 CDS CHO2 NC_001139.8 802445 805054 D Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis; Cho2p 802445..805054 Saccharomyces cerevisiae 853061 NP_011674.1 CDS MTR3 NC_001139.8 805274 806026 R Mtr3p; 3'5' exoribonuclease, exosome subunit; nucleolar protein involved in export of mRNA and ribosomal subunits; homologous to the E. coli exonuclease RNase PH complement(805274..806026) Saccharomyces cerevisiae 853062 NP_011675.1 CDS NSR1 NC_001139.8 806417 807661 R Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis; Nsr1p complement(806417..807661) Saccharomyces cerevisiae 853064 NP_011677.1 CDS RTS3 NC_001139.8 808634 809425 R Rts3p; Putative component of the protein phosphatase type 2A complex complement(808634..809425) Saccharomyces cerevisiae 853065 NP_001018033.1 CDS YGR161W-C NC_001139.8 810232 810510 D Ygr161w-cp; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species 810232..810510 Saccharomyces cerevisiae 3077355 NP_058163.1 CDS YGR161W-B NC_001139.8 811743 817056 D Ygr161w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(811743..813035,813037..817056) Saccharomyces cerevisiae 853067 NP_058164.1 CDS YGR161W-A NC_001139.8 811743 813059 D Ygr161w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 811743..813059 Saccharomyces cerevisiae 853066 NP_058165.1 CDS YGR161C-D NC_001139.8 817752 823020 R Ygr161c-dp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(817752..821714,821716..823020)) Saccharomyces cerevisiae 853068 NP_058166.1 CDS YGR161C-C NC_001139.8 821698 823020 R Ygr161c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(821698..823020) Saccharomyces cerevisiae 853069 NP_011678.1 CDS TIF4631 NC_001139.8 824064 826922 D Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p; Tif4631p 824064..826922 Saccharomyces cerevisiae 853071 NP_011679.1 CDS GTR2 NC_001139.8 827557 828582 D Gtr2p; Cytoplasmic GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; subunit of EGO complex, which is involved in microautophagy regulation, and of GSE complex, required for sorting of Gap1p; homolog of human RagC and RagD 827557..828582 Saccharomyces cerevisiae 853072 NP_011681.1 CDS MRPS35 NC_001139.8 829121 830158 D Mrps35p; Mitochondrial ribosomal protein of the small subunit 829121..830158 Saccharomyces cerevisiae 853075 NP_011682.1 CDS KRE11 NC_001139.8 830520 832202 D Protein involved in biosynthesis of cell wall beta-glucans; subunit of the TRAPP (transport protein particle) complex, which is involved in the late steps of endoplasmic reticulum to Golgi transport; Kre11p 830520..832202 Saccharomyces cerevisiae 853076 NP_011683.1 CDS CLC1 NC_001139.8 832461 833162 D Clc1p; Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function, two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component 832461..833162 Saccharomyces cerevisiae 853077 NP_011684.1 CDS YGR168C NC_001139.8 833357 834487 R Ygr168cp; Putative protein of unknown function; YGR168C is not an essential gene complement(833357..834487) Saccharomyces cerevisiae 853078 NP_011685.1 CDS PUS6 NC_001139.8 834694 835908 R Pus6p; Pseudouridine synthase responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability complement(834694..835908) Saccharomyces cerevisiae 853079 NP_878080.1 CDS YGR169C-A NC_001139.8 836392 836670 R Ygr169c-ap; Putative protein of unknown function complement(836392..836670) Saccharomyces cerevisiae 1466460 NP_011686.1 CDS PSD2 NC_001139.8 837147 840563 D Psd2p; Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine 837147..840563 Saccharomyces cerevisiae 853080 NP_011687.1 CDS MSM1 NC_001139.8 840829 842556 R Msm1p; Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p complement(840829..842556) Saccharomyces cerevisiae 853081 NP_011688.1 CDS YIP1 NC_001139.8 842850 843596 R Yip1p; Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles complement(842850..843596) Saccharomyces cerevisiae 853082 NP_011689.1 CDS RBG2 NC_001139.8 843859 844965 D Rbg2p; Protein with similarity to mammalian developmentally regulated GTP-binding protein 843859..844965 Saccharomyces cerevisiae 853083 NP_011690.1 CDS CBP4 NC_001139.8 845898 846410 R Mitochondrial protein required for assembly of ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); interacts with Cbp3p and function is partially redundant with that of Cbp3p; Cbp4p complement(845898..846410) Saccharomyces cerevisiae 853085 NP_878081.1 CDS YGR174W-A NC_001139.8 846660 846746 D Ygr174w-ap; Putative protein of unknown function 846660..846746 Saccharomyces cerevisiae 1466461 NP_011691.1 CDS ERG1 NC_001139.8 846938 848428 R Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine; Erg1p complement(846938..848428) Saccharomyces cerevisiae 853086 NP_011693.1 CDS ATF2 NC_001139.8 848834 850441 R Atf2p; Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important in brewing complement(848834..850441) Saccharomyces cerevisiae 853088 NP_011694.1 CDS PBP1 NC_001139.8 851052 853220 R Protein interacting with poly(A)-binding protein Pab1p; likely involved in controlling the extent of mRNA polyadenylation; forms a complex with Mkt1p that may regulate HO translation; interacts with Lsm12p in a copurification assay; Pbp1p complement(851052..853220) Saccharomyces cerevisiae 853089 NP_011695.1 CDS OKP1 NC_001139.8 853680 854900 R Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; Okp1p complement(853680..854900) Saccharomyces cerevisiae 853090 NP_011696.1 CDS RNR4 NC_001139.8 855269 856306 R Rnr4p; Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits complement(855269..856306) Saccharomyces cerevisiae 853091 NP_011697.1 CDS TIM13 NC_001139.8 858292 858609 D Tim13p; Mitochondrial intermembrane space protein, forms a complex with TIm8p that mediates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner 858292..858609 Saccharomyces cerevisiae 853093 NP_011699.1 CDS QCR9 NC_001139.8 859068 859481 R Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex; Qcr9p complement(join(859068..859265,859479..859481)) Saccharomyces cerevisiae 853095 NP_011700.1 CDS UBR1 NC_001139.8 859906 865758 R Ubr1p; Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle of the 26S proteasome complement(859906..865758) Saccharomyces cerevisiae 853096 NP_011701.1 CDS TYS1 NC_001139.8 866341 867525 R Tys1p; Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr complement(866341..867525) Saccharomyces cerevisiae 853097 NP_011702.1 CDS TFG1 NC_001139.8 867779 869986 D Tfg1p; TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 867779..869986 Saccharomyces cerevisiae 853098 NP_011703.1 CDS HGH1 NC_001139.8 870237 871421 R Protein of unknown function with similarity to human HMG1 and HMG2; localizes to the cytoplasm; Hgh1p complement(870237..871421) Saccharomyces cerevisiae 853099 NP_011704.1 CDS BUB1 NC_001139.8 872049 875114 R Protein kinase that forms a complex with Mad1p and Bub3p that is crucial in the checkpoint mechanism required to prevent cell cycle progression into anaphase in the presence of spindle damage, associates with centromere DNA via Skp1p; Bub1p complement(872049..875114) Saccharomyces cerevisiae 853100 NP_011705.1 CDS CRH1 NC_001139.8 876674 878197 R Crh1p; Cell wall protein that functions in the transfer of chitin to beta(1-6)glucan, putative chitin transglycosidase complement(876674..878197) Saccharomyces cerevisiae 853102 NP_011707.1 CDS HIP1 NC_001139.8 880425 882236 D Hip1p; High-affinity histidine permease, also involved in the transport of manganese ions 880425..882236 Saccharomyces cerevisiae 853104 NP_011708.1 CDS TDH3 NC_001139.8 882817 883815 R Tdh3p; Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall complement(882817..883815) Saccharomyces cerevisiae 853106 NP_011709.1 CDS PDX1 NC_001139.8 884514 885746 R Pdx1p; Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core complement(884514..885746) Saccharomyces cerevisiae 853107 NP_011710.1 CDS XKS1 NC_001139.8 886078 887880 R Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains; Xks1p complement(886078..887880) Saccharomyces cerevisiae 853108 NP_011711.1 CDS SKI6 NC_001139.8 888887 889627 D Ski6p; 3'-to-5' phosphorolytic exoribonuclease that is a subunit of the exosome; required for 3' processing of the 5.8S rRNA; involved in 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs 888887..889627 Saccharomyces cerevisiae 853109 NP_011712.1 CDS FYV8 NC_001139.8 889738 892191 R Protein of unknown function, required for survival upon exposure to K1 killer toxin; Fyv8p complement(889738..892191) Saccharomyces cerevisiae 853110 NP_011713.1 CDS SNG1 NC_001139.8 892502 894145 R Sng1p; Protein involved in nitrosoguanidine (MNNG) resistance; expression is regulated by transcription factors involved in multidrug resistance complement(892502..894145) Saccharomyces cerevisiae 853111 NP_011714.1 CDS YPP1 NC_001139.8 894698 897151 D Ypp1p; Cargo-transport protein involved in endocytosis; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene 894698..897151 Saccharomyces cerevisiae 853112 NP_011715.1 CDS PMT6 NC_001139.8 897507 899786 D Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases; Pmt6p 897507..899786 Saccharomyces cerevisiae 853113 NP_011716.1 CDS ELP2 NC_001139.8 899909 902275 R Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin; Elp2p complement(899909..902275) Saccharomyces cerevisiae 853114 NP_011717.2 CDS YGR201C NC_001139.8 902525 903202 R Ygr201cp; Putative protein of unknown function complement(902525..903202) Saccharomyces cerevisiae 853115 NP_011718.1 CDS PCT1 NC_001139.8 903479 904753 R Pct1p; Cholinephosphate cytidylyltransferase, also known as CTP:phosphocholine cytidylyltransferase, rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding complement(903479..904753) Saccharomyces cerevisiae 853116 NP_011719.1 CDS YGR203W NC_001139.8 905242 905688 D Ygr203wp; Putative protein with sequence similarity to protein tyrosine phosphatases of the CDC25-like phosphatase family; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 905242..905688 Saccharomyces cerevisiae 853117 NP_011720.1 CDS ADE3 NC_001139.8 905939 908779 D Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; Ade3p 905939..908779 Saccharomyces cerevisiae 853118 NP_878082.1 CDS YGR204C-A NC_001139.8 909066 909179 R Ygr204c-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(909066..909179) Saccharomyces cerevisiae 1466462 NP_011721.1 CDS YGR205W NC_001139.8 909218 910090 D Ygr205wp; ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases 909218..910090 Saccharomyces cerevisiae 853119 NP_011722.1 CDS MVB12 NC_001139.8 910437 910742 D Mvb12p; ESCRT-I subunit necessary for the efficient transport of cargo proteins to the vacuolar lumen; deletion mutant sensitive to rapamycin and nystatin; green fluorescent protein (GFP)-fusion protein localizes to endosome 910437..910742 Saccharomyces cerevisiae 853120 NP_011723.1 CDS YGR207C NC_001139.8 910848 911633 R Ygr207cp; Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavoprotein complex subunit ETF-beta; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(910848..911633) Saccharomyces cerevisiae 853121 NP_011724.1 CDS SER2 NC_001139.8 911888 912817 D Ser2p; Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source 911888..912817 Saccharomyces cerevisiae 853122 NP_011725.1 CDS TRX2 NC_001139.8 912918 913232 R Trx2p; Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance complement(912918..913232) Saccharomyces cerevisiae 853123 NP_011726.1 CDS YGR210C NC_001139.8 913508 914743 R Ygr210cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(913508..914743) Saccharomyces cerevisiae 853124 NP_011727.1 CDS ZPR1 NC_001139.8 915246 916706 D Zpr1p; Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p) 915246..916706 Saccharomyces cerevisiae 853125 NP_011728.1 CDS SLI1 NC_001139.8 917045 918451 D Sli1p; N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin 917045..918451 Saccharomyces cerevisiae 853126 NP_011729.1 CDS RTA1 NC_001139.8 918517 919470 R Rta1p; Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions complement(918517..919470) Saccharomyces cerevisiae 853127 NP_011730.1 CDS RPS0A NC_001139.8 920580 921793 D Rps0ap; Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal join(920580..920669,921125..921793) Saccharomyces cerevisiae 853128 NP_011731.1 CDS RSM27 NC_001139.8 922180 922512 D Rsm27p; Mitochondrial ribosomal protein of the small subunit 922180..922512 Saccharomyces cerevisiae 853129 NP_011732.1 CDS GPI1 NC_001139.8 922638 924467 R Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs; Gpi1p complement(922638..924467) Saccharomyces cerevisiae 853130 NP_011733.1 CDS CCH1 NC_001139.8 924699 930818 D Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together; Cch1p 924699..930818 Saccharomyces cerevisiae 853131 NP_011734.1 CDS CRM1 NC_001139.8 932544 935798 D Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; Crm1p 932544..935798 Saccharomyces cerevisiae 853133 NP_011736.1 CDS MRPL9 NC_001139.8 936077 936886 R Mrpl9p; Mitochondrial ribosomal protein of the large subunit complement(936077..936886) Saccharomyces cerevisiae 853135 NP_011737.1 CDS TOS2 NC_001139.8 937127 938995 R Tos2p; Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p complement(937127..938995) Saccharomyces cerevisiae 853136 NP_011738.1 CDS PET54 NC_001139.8 939926 940807 D Pet54p; Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet122p and Pet494p; also required for splicing of the COX1 intron AI5 beta; located in the mitochondrial inner membrane 939926..940807 Saccharomyces cerevisiae 853137 NP_011739.1 CDS HSV2 NC_001139.8 940872 942218 R Phosphatidylinositol 3,5-bisphosphate-binding protein, predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization; Hsv2p complement(940872..942218) Saccharomyces cerevisiae 853138 NP_011740.1 CDS AZR1 NC_001139.8 942809 944650 D Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole; Azr1p 942809..944650 Saccharomyces cerevisiae 853139 NP_011741.3 CDS AMA1 NC_001139.8 945148 947022 D Ama1p; Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis join(945148..946330,946424..947022) Saccharomyces cerevisiae 853140 NP_011743.1 CDS DIE2 NC_001139.8 947423 949000 D Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1; Die2p 947423..949000 Saccharomyces cerevisiae 853142 NP_011745.1 CDS SMI1 NC_001139.8 949385 950902 R Smi1p; Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity complement(949385..950902) Saccharomyces cerevisiae 853144 NP_011746.1 CDS BNS1 NC_001139.8 951897 952310 D Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis; Bns1p 951897..952310 Saccharomyces cerevisiae 853145 NP_011747.2 CDS PHB2 NC_001139.8 952551 953483 R Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; Phb2p complement(952551..953483) Saccharomyces cerevisiae 853146 NP_011748.1 CDS NAS6 NC_001139.8 953963 954649 D Regulatory, non-ATPase subunit of the 26S proteasome; homolog of the human oncoprotein gankyrin, which interacts with the retinoblastoma tumor suppressor (Rb) and cyclin-dependent kinase 4/6; Nas6p 953963..954649 Saccharomyces cerevisiae 853147 NP_011749.1 CDS PHO81 NC_001139.8 954677 958213 R Pho81p; Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p complement(954677..958213) Saccharomyces cerevisiae 853148 NP_011750.1 CDS YHB1 NC_001139.8 959908 961107 D Yhb1p; Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses 959908..961107 Saccharomyces cerevisiae 853149 NP_011751.1 CDS YGR235C NC_001139.8 961364 962065 R Ygr235cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(961364..962065) Saccharomyces cerevisiae 853150 NP_011752.2 CDS SPG1 NC_001139.8 962534 962821 R Spg1p; Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(962534..962821) Saccharomyces cerevisiae 853151 NP_011753.1 CDS YGR237C NC_001139.8 963303 965660 R Ygr237cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(963303..965660) Saccharomyces cerevisiae 853152 NP_011754.1 CDS KEL2 NC_001139.8 966044 968692 R Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; Kel2p complement(966044..968692) Saccharomyces cerevisiae 853153 NP_011755.1 CDS PEX21 NC_001139.8 969192 970058 R Pex21p; Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex18p complement(969192..970058) Saccharomyces cerevisiae 853154 NP_011756.1 CDS PFK1 NC_001139.8 970776 973739 R Pfk1p; Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes complement(970776..973739) Saccharomyces cerevisiae 853155 NP_878083.1 CDS YGR240C-A NC_001139.8 974582 974782 R Ygr240c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(974582..974782) Saccharomyces cerevisiae 1466463 NP_011757.1 CDS YAP1802 NC_001139.8 974880 976586 R Yap1802p; Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1801p, member of the AP180 protein family complement(974880..976586) Saccharomyces cerevisiae 853157 NP_011759.1 CDS FMP43 NC_001139.8 977341 977781 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp43p 977341..977781 Saccharomyces cerevisiae 853158 NP_011760.1 CDS LSC2 NC_001139.8 978041 979324 R Lsc2p; Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate complement(978041..979324) Saccharomyces cerevisiae 853159 NP_011761.1 CDS SDA1 NC_001139.8 979770 982073 R Sda1p; Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis complement(979770..982073) Saccharomyces cerevisiae 853160 NP_011762.1 CDS BRF1 NC_001139.8 982487 984277 R TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; Brf1p complement(982487..984277) Saccharomyces cerevisiae 853161 NP_011763.1 CDS CPD1 NC_001139.8 984971 985690 D Cyclic nucleotide phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; Cpd1p 984971..985690 Saccharomyces cerevisiae 853162 NP_011764.1 CDS SOL4 NC_001139.8 985977 986744 D Sol4p; 6-phosphogluconolactonase with similarity to Sol3p 985977..986744 Saccharomyces cerevisiae 853163 NP_011765.1 CDS MGA1 NC_001139.8 988054 989424 D Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants; Mga1p 988054..989424 Saccharomyces cerevisiae 853164 NP_011766.1 CDS YGR250C NC_001139.8 991181 993526 R Ygr250cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(991181..993526) Saccharomyces cerevisiae 853165 NP_011767.1 CDS YGR251W NC_001139.8 995644 996234 D Ygr251wp; Putative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the nucleolus; YGR251W is an essential gene 995644..996234 Saccharomyces cerevisiae 853166 NP_011768.1 CDS GCN5 NC_001139.8 996874 998193 D Histone acetyltransferase, acetylates N-terminal lysines on histones H2B and H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily; Gcn5p 996874..998193 Saccharomyces cerevisiae 853167 NP_011769.1 CDS PUP2 NC_001139.8 998363 999145 R Pup2p; Alpha subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta complement(998363..999145) Saccharomyces cerevisiae 853168 NP_011770.1 CDS ENO1 NC_001139.8 1000932 1002245 D Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; Eno1p 1000932..1002245 Saccharomyces cerevisiae 853169 NP_011771.1 CDS COQ6 NC_001139.8 1002528 1003967 R Putative flavin-dependent monooxygenase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; Coq6p complement(1002528..1003967) Saccharomyces cerevisiae 853170 NP_011772.1 CDS GND2 NC_001139.8 1004630 1006108 D Gnd2p; 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone 1004630..1006108 Saccharomyces cerevisiae 853172 NP_011773.1 CDS MTM1 NC_001139.8 1006211 1007311 R Mtm1p; Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor complement(1006211..1007311) Saccharomyces cerevisiae 853173 NP_011774.1 CDS RAD2 NC_001139.8 1007677 1010772 R Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein; Rad2p complement(1007677..1010772) Saccharomyces cerevisiae 853174 NP_011776.1 CDS TNA1 NC_001139.8 1012491 1014095 D Tna1p; High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) 1012491..1014095 Saccharomyces cerevisiae 853175 NP_011777.1 CDS APL6 NC_001139.8 1014327 1016756 R Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools; Apl6p complement(1014327..1016756) Saccharomyces cerevisiae 853177 NP_011778.1 CDS BUD32 NC_001139.8 1016980 1017765 R Protein kinase proposed to be involved in bud-site selection, telomere uncapping and elongation, and transcription; component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Bud32p complement(1016980..1017765) Saccharomyces cerevisiae 853178 NP_011779.1 CDS SAY1 NC_001139.8 1017973 1019247 R Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; Say1p complement(1017973..1019247) Saccharomyces cerevisiae 853179 NP_011780.1 CDS MES1 NC_001139.8 1019604 1021859 R Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs; Mes1p complement(1019604..1021859) Saccharomyces cerevisiae 853181 NP_011782.1 CDS YGR266W NC_001139.8 1022662 1024767 D Ygr266wp; Protein of unknown function, predicted to contain a single transmembrane domain; localized to both the mitochondrial outer membrane and the plasma membrane 1022662..1024767 Saccharomyces cerevisiae 853182 NP_011783.1 CDS FOL2 NC_001139.8 1025010 1025741 R GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway; Fol2p complement(1025010..1025741) Saccharomyces cerevisiae 853183 NP_011784.1 CDS HUA1 NC_001139.8 1026063 1026659 R Hua1p; Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly complement(1026063..1026659) Saccharomyces cerevisiae 853185 NP_011786.1 CDS YTA7 NC_001139.8 1027376 1031515 D Yta7p; Protein of unknown function, member of CDC48/PAS1/SEC18 family of ATPases, potentially phosphorylated by Cdc28p 1027376..1031515 Saccharomyces cerevisiae 853186 NP_011787.1 CDS SLH1 NC_001139.8 1031797 1037700 D Slh1p; Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses 1031797..1037700 Saccharomyces cerevisiae 853187 NP_076891.2 CDS EFG1 NC_001139.8 1037806 1038507 R Protein of unknown function; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; Efg1p complement(1037806..1038507) Saccharomyces cerevisiae 853188 NP_011789.1 CDS YGR273C NC_001139.8 1038721 1039245 R Ygr273cp; Putative protein of unknown function; deletion mutant has no readily detectable phenotype complement(1038721..1039245) Saccharomyces cerevisiae 853190 NP_011790.1 CDS TAF1 NC_001139.8 1039902 1043102 R Taf1p; TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation, has histone acetyltransferase activity, involved in promoter binding and G1/S progression complement(1039902..1043102) Saccharomyces cerevisiae 853191 NP_011791.2 CDS RTT102 NC_001139.8 1043283 1043756 D Rtt102p; Component of both the SWI/SNF and RSC chromatin remodeling complexes, suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition 1043283..1043756 Saccharomyces cerevisiae 853192 NP_011792.1 CDS RNH70 NC_001139.8 1043826 1045487 R Rnh70p; 3' exoribonuclease, required for 5S rRNA and tRNA-Arg3 maturation complement(1043826..1045487) Saccharomyces cerevisiae 853193 NP_011793.1 CDS YGR277C NC_001139.8 1045651 1046568 R Ygr277cp; Putative pantetheine-phosphate adenylyltransferase (PPAT) that catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; conserved in bacteria, humans, and plants; essential for viability complement(1045651..1046568) Saccharomyces cerevisiae 853194 NP_011794.1 CDS CWC22 NC_001139.8 1046738 1048471 D Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data; Cwc22p 1046738..1048471 Saccharomyces cerevisiae 853195 NP_011795.1 CDS SCW4 NC_001139.8 1048805 1049965 R Scw4p; Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating complement(1048805..1049965) Saccharomyces cerevisiae 853196 NP_011796.1 CDS PXR1 NC_001139.8 1050917 1051732 R Pxr1p; Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain complement(1050917..1051732) Saccharomyces cerevisiae 853197 NP_011797.1 CDS YOR1 NC_001139.8 1052831 1057264 D Yor1p; Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin 1052831..1057264 Saccharomyces cerevisiae 853198 NP_011798.1 CDS BGL2 NC_001139.8 1057790 1058731 R Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance; Bgl2p complement(1057790..1058731) Saccharomyces cerevisiae 853199 NP_011799.1 CDS YGR283C NC_001139.8 1059022 1060047 R Ygr283cp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; deletion mutant is resistant to fluconazole; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus complement(1059022..1060047) Saccharomyces cerevisiae 853200 NP_011800.1 CDS ERV29 NC_001139.8 1060665 1061597 R Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; Erv29p complement(1060665..1061597) Saccharomyces cerevisiae 853201 NP_011801.1 CDS ZUO1 NC_001139.8 1061859 1063160 R Zuo1p; Cytosolic ribosome-associated chaperone that acts, together with Ssz1p and the Ssb proteins, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p complement(1061859..1063160) Saccharomyces cerevisiae 853202 NP_011802.1 CDS BIO2 NC_001139.8 1063820 1064947 R Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant; Bio2p complement(1063820..1064947) Saccharomyces cerevisiae 853203 NP_011803.1 CDS YGR287C NC_001139.8 1067229 1068998 R Ygr287cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to alpha-D-glucosidase (maltase); authentic, non-tagged protein detected in purified mitochondria in high-throughput studies complement(1067229..1068998) Saccharomyces cerevisiae 853204 NP_011804.1 CDS MAL13 NC_001139.8 1070300 1071721 D Mal13p; MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C 1070300..1071721 Saccharomyces cerevisiae 853205 NP_011805.1 CDS MAL11 NC_001139.8 1073970 1075820 R Maltose permease, inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; Mal11p complement(1073970..1075820) Saccharomyces cerevisiae 853207 NP_011808.1 CDS MAL12 NC_001139.8 1076606 1078360 D Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; Mal12p 1076606..1078360 Saccharomyces cerevisiae 853209 NP_011810.1 CDS PAU12 NC_001139.8 1080313 1080675 D Pau12p 1080313..1080675 Saccharomyces cerevisiae 853210 NP_011811.1 CDS COS6 NC_001139.8 1081591 1082736 R Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos6p complement(1081591..1082736) Saccharomyces cerevisiae 853212 NP_011812.1 CDS YRF1-3 NC_001139.8 1084871 1090598 D Yrf1-3p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p join(1084871..1084889,1085038..1090598) Saccharomyces cerevisiae 853213 NP_011813.1 CDS YHL050C NC_001140.5 445 3310 R Yhl050cp; Putative protein of unknown function, potential Cdc28p substrate complement(join(445..1897,2670..3310)) Saccharomyces cerevisiae 856335 NP_011814.1 CDS YHL049C NC_001140.5 3725 4540 R Yhl049cp; Putative protein of unknown function complement(3725..4540) Saccharomyces cerevisiae 856336 NP_878084.1 CDS YHL048C-A NC_001140.5 5662 5796 R Yhl048c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(5662..5796) Saccharomyces cerevisiae 1466524 NP_011815.1 CDS COS8 NC_001140.5 6400 7545 D Nuclear membrane protein, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response; Cos8p 6400..7545 Saccharomyces cerevisiae 856337 NP_011816.1 CDS ARN2 NC_001140.5 8298 10211 R Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C; Arn2p complement(8298..10211) Saccharomyces cerevisiae 856338 NP_011817.1 CDS PAU13 NC_001140.5 11921 12283 R Putative protein of unknown function; not an essential gene; Pau13p complement(11921..12283) Saccharomyces cerevisiae 856339 NP_011819.1 CDS YHL044W NC_001140.5 13563 14270 D Yhl044wp; Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern 13563..14270 Saccharomyces cerevisiae 856341 NP_011820.1 CDS ECM34 NC_001140.5 14899 15411 D Putative protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Ecm34p 14899..15411 Saccharomyces cerevisiae 856342 NP_011821.1 CDS YHL042W NC_001140.5 15665 16117 D Yhl042wp; Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins 15665..16117 Saccharomyces cerevisiae 856343 NP_011823.1 CDS ARN1 NC_001140.5 19085 20968 R Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; Arn1p complement(19085..20968) Saccharomyces cerevisiae 856345 NP_011824.1 CDS YHL039W NC_001140.5 21780 23537 D Yhl039wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 21780..23537 Saccharomyces cerevisiae 856346 NP_011825.1 CDS CBP2 NC_001140.5 23614 25506 R Mitochondrial protein required for splicing of the group I intron aI5 of the COB pre-mRNA, binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene; Cbp2p complement(23614..25506) Saccharomyces cerevisiae 856347 NP_011827.1 CDS MUP3 NC_001140.5 26239 27879 D Mup3p; Low affinity methionine permease, similar to Mup1p 26239..27879 Saccharomyces cerevisiae 856349 NP_011828.1 CDS VMR1 NC_001140.5 27976 32754 R Vmr1p; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ATP-binding cassette (ABC) family; potential Cdc28p substrate; detected in purified mitochondria in high-throughput studies complement(27976..32754) Saccharomyces cerevisiae 856350 NP_011829.1 CDS SBP1 NC_001140.5 33191 34075 R Sbp1p; Putative RNA binding protein; involved in translational repression and found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 complement(33191..34075) Saccharomyces cerevisiae 856351 NP_011830.1 CDS RPL8A NC_001140.5 35253 36023 R Rpl8ap; Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits complement(35253..36023) Saccharomyces cerevisiae 856352 NP_011831.1 CDS GUT1 NC_001140.5 36377 38506 R Gut1p; Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p complement(36377..38506) Saccharomyces cerevisiae 856353 NP_011832.1 CDS GOS1 NC_001140.5 38813 39484 R v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28; Gos1p complement(38813..39484) Saccharomyces cerevisiae 856354 NP_011833.1 CDS ECM29 NC_001140.5 40082 45688 D Major component of the proteasome; tethers the proteasome core particle to the regulatory particle, and enhances the stability of the proteasome; Ecm29p 40082..45688 Saccharomyces cerevisiae 856355 NP_011834.1 CDS OCA5 NC_001140.5 45927 47966 R Oca5p; Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts complement(45927..47966) Saccharomyces cerevisiae 856356 NP_011835.1 CDS WSC4 NC_001140.5 48761 50578 D Wsc4p; ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 48761..50578 Saccharomyces cerevisiae 856357 NP_011836.1 CDS RIM101 NC_001140.5 51109 52986 D Transcriptional repressor involved in response to pH and in cell wall construction; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by proteolytic processing; similar to A. nidulans PacC; Rim101p 51109..52986 Saccharomyces cerevisiae 856358 NP_011837.2 CDS YHL026C NC_001140.5 53217 54164 R Yhl026cp; Putative protein of unknown function; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) complement(53217..54164) Saccharomyces cerevisiae 856359 NP_011838.1 CDS SNF6 NC_001140.5 54849 55847 D Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; Snf6p 54849..55847 Saccharomyces cerevisiae 856360 NP_011839.1 CDS RIM4 NC_001140.5 56647 58788 D Rim4p; Putative RNA-binding protein required for the expression of early and middle sporulation genes 56647..58788 Saccharomyces cerevisiae 856361 NP_011840.1 CDS RMD11 NC_001140.5 59121 62561 R Rmd11p; Protein required for sporulation complement(59121..62561) Saccharomyces cerevisiae 856362 NP_011841.1 CDS SPO11 NC_001140.5 62959 64155 R Spo11p; Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation complement(62959..64155) Saccharomyces cerevisiae 856364 NP_011842.1 CDS AIM17 NC_001140.5 64459 65856 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss (petite formation); Aim17p complement(64459..65856) Saccharomyces cerevisiae 856365 NP_011843.1 CDS OPI1 NC_001140.5 66239 67453 R Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; Opi1p complement(66239..67453) Saccharomyces cerevisiae 856366 NP_011844.1 CDS APM2 NC_001140.5 67728 69545 R Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport; Apm2p complement(67728..69545) Saccharomyces cerevisiae 856367 NP_011845.1 CDS YHL018W NC_001140.5 69705 70067 D Yhl018wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS 69705..70067 Saccharomyces cerevisiae 856368 NP_011846.1 CDS YHL017W NC_001140.5 70273 71871 D Yhl017wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles 70273..71871 Saccharomyces cerevisiae 856369 NP_011847.1 CDS DUR3 NC_001140.5 72034 74241 R Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway; Dur3p complement(72034..74241) Saccharomyces cerevisiae 856370 NP_878085.1 CDS YHL015W-A NC_001140.5 74696 74779 D Yhl015w-ap; Putative protein of unknown function 74696..74779 Saccharomyces cerevisiae 1466525 NP_011848.1 CDS RPS20 NC_001140.5 75409 75774 D Rps20p; Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins 75409..75774 Saccharomyces cerevisiae 856371 NP_011849.1 CDS YLF2 NC_001140.5 76094 77311 R Ylf2p; Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(76094..77311) Saccharomyces cerevisiae 856372 NP_011850.1 CDS OTU2 NC_001140.5 77427 78350 R Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; Otu2p complement(77427..78350) Saccharomyces cerevisiae 856373 NP_011851.1 CDS YHL012W NC_001140.5 78932 80413 D Yhl012wp; Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase 78932..80413 Saccharomyces cerevisiae 856374 NP_011852.1 CDS PRS3 NC_001140.5 80650 81612 R Prs3p; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes complement(80650..81612) Saccharomyces cerevisiae 856375 NP_011853.1 CDS YHL010C NC_001140.5 81960 83717 R Yhl010cp; Putative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences complement(81960..83717) Saccharomyces cerevisiae 856376 NP_011854.1 CDS YAP3 NC_001140.5 84064 85056 R Yap3p; Basic leucine zipper (bZIP) transcription factor complement(84064..85056) Saccharomyces cerevisiae 856377 NP_058133.1 CDS YHL009W-B NC_001140.5 85905 91314 D Yhl009w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(85905..86990,86992..91314) Saccharomyces cerevisiae 856380 NP_058134.1 CDS YHL009W-A NC_001140.5 85905 87146 D Yhl009w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 85905..87146 Saccharomyces cerevisiae 856379 NP_011855.1 CDS YHL008C NC_001140.5 92623 94506 R Yhl008cp; Putative protein of unknown function, does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole complement(92623..94506) Saccharomyces cerevisiae 856381 NP_011856.1 CDS STE20 NC_001140.5 95114 97933 R Ste20p; Signal transducing kinase of the PAK (p21-activated kinase) family, involved in pheromone response and pseudohyphal/invasive growth pathways, activated by Cdc42p; binds Ste4p at a GBB motif present in noncatalytic domains of PAK kinases complement(95114..97933) Saccharomyces cerevisiae 856382 NP_011857.2 CDS SHU1 NC_001140.5 98339 98791 R Shu1p; Protein of unassigned function involved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation; associates with Shu2p, Psy3p, and Csm2p complement(98339..98791) Saccharomyces cerevisiae 856383 NP_011859.1 CDS MRP4 NC_001140.5 99215 100399 D Mrp4p; Mitochondrial ribosomal protein of the small subunit 99215..100399 Saccharomyces cerevisiae 856384 NP_011860.1 CDS LAG1 NC_001140.5 100644 101879 R Lag1p; Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p complement(100644..101879) Saccharomyces cerevisiae 856386 NP_011861.1 CDS HSE1 NC_001140.5 102607 103965 D Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes; Hse1p 102607..103965 Saccharomyces cerevisiae 856387 NP_011862.1 CDS RPL14B NC_001140.5 104272 105086 D Rpl14bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similarity to rat L14 ribosomal protein join(104272..104400,104799..105086) Saccharomyces cerevisiae 856388 NP_011863.1 CDS OSH7 NC_001140.5 106050 107363 D Osh7p; Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability 106050..107363 Saccharomyces cerevisiae 856389 NP_011864.1 CDS QCR10 NC_001140.5 107821 108117 D Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain; Qcr10p join(107821..107826,107890..108117) Saccharomyces cerevisiae 856390 NP_011865.1 CDS LEU5 NC_001140.5 108807 109880 D Mitochondrial carrier protein involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized; Leu5p 108807..109880 Saccharomyces cerevisiae 856391 NP_011866.1 CDS YHR003C NC_001140.5 110023 111312 R Yhr003cp; Protein of unknown function, localized to the mitochondrial outer membrane complement(110023..111312) Saccharomyces cerevisiae 856392 NP_011867.1 CDS NEM1 NC_001140.5 111749 113089 R Nem1p; Catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic genes; required for normal nuclear envelope morphology and sporulation complement(111749..113089) Saccharomyces cerevisiae 856393 NP_011868.1 CDS GPA1 NC_001140.5 113494 114912 R Gpa1p; GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome complement(113494..114912) Saccharomyces cerevisiae 856394 NP_011869.1 CDS MRS11 NC_001140.5 115615 115896 R Mrs11p; Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that mediates insertion of hydrophobic proteins at the inner membrane, has homology to Mrs5p, which is also involved in this process complement(115615..115896) Saccharomyces cerevisiae 856395 NP_011870.1 CDS STP2 NC_001140.5 117809 119434 D Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; Stp2p 117809..119434 Saccharomyces cerevisiae 856397 NP_011871.1 CDS ERG11 NC_001140.5 120086 121678 R Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; Erg11p complement(120086..121678) Saccharomyces cerevisiae 856398 NP_878086.1 CDS YHR007C-A NC_001140.5 122545 122760 R Yhr007c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(122545..122760) Saccharomyces cerevisiae 1466526 NP_011872.1 CDS SOD2 NC_001140.5 122884 123585 R Sod2p; Mitochondrial superoxide dismutase, protects cells against oxygen toxicity; phosphorylated complement(122884..123585) Saccharomyces cerevisiae 856399 NP_011873.1 CDS YHR009C NC_001140.5 124104 125675 R Yhr009cp; Putative protein of unknown function; not an essential gene complement(124104..125675) Saccharomyces cerevisiae 856400 NP_011874.1 CDS RPL27A NC_001140.5 126515 127486 D Rpl27ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similarity to rat L27 ribosomal protein join(126515..126545,127107..127486) Saccharomyces cerevisiae 856401 NP_011875.1 CDS DIA4 NC_001140.5 127774 129114 D Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth; Dia4p 127774..129114 Saccharomyces cerevisiae 856402 NP_011876.1 CDS VPS29 NC_001140.5 129475 130442 D Vps29p; Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval join(129475..129522,129642..130442) Saccharomyces cerevisiae 856403 NP_011877.1 CDS ARD1 NC_001140.5 130724 131440 R Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing; Ard1p complement(130724..131440) Saccharomyces cerevisiae 856404 NP_011878.1 CDS SPO13 NC_001140.5 132040 132915 D Spo13p; Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II 132040..132915 Saccharomyces cerevisiae 856405 NP_011879.1 CDS MIP6 NC_001140.5 134547 136526 D Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; Mip6p 134547..136526 Saccharomyces cerevisiae 856408 NP_011880.1 CDS YSC84 NC_001140.5 136874 138448 R Ysc84p; Protein involved in the organization of the actin cytoskeleton; contains SH3 domain similar to Rvs167p complement(join(136874..138233,138402..138448)) Saccharomyces cerevisiae 856409 NP_011881.1 CDS YSC83 NC_001140.5 138687 139844 D Ysc83p; Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 138687..139844 Saccharomyces cerevisiae 856410 NP_011882.1 CDS ARG4 NC_001140.5 140004 141395 R Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway; Arg4p complement(140004..141395) Saccharomyces cerevisiae 856411 NP_011883.1 CDS DED81 NC_001140.5 141887 143551 R Ded81p; Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA complement(141887..143551) Saccharomyces cerevisiae 856412 NP_011884.1 CDS YHR020W NC_001140.5 143989 146055 D Yhr020wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene 143989..146055 Saccharomyces cerevisiae 856413 NP_011885.1 CDS RPS27B NC_001140.5 147864 148662 R Rps27bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Ap and has similarity to rat S27 ribosomal protein complement(join(147864..148109,148660..148662)) Saccharomyces cerevisiae 856415 NP_011886.1 CDS ECM12 NC_001140.5 149218 149673 D Non-essential protein of unknown function; Ecm12p 149218..149673 Saccharomyces cerevisiae 856416 NP_011887.1 CDS YHR022C NC_001140.5 149568 150338 R Yhr022cp; Putative protein of unknown function; YHR022C is not an essential gene. complement(149568..150338) Saccharomyces cerevisiae 856417 NP_878087.1 CDS YHR022C-A NC_001140.5 151210 151299 R Yhr022c-ap; Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(151210..151299) Saccharomyces cerevisiae 1466527 NP_011888.1 CDS MYO1 NC_001140.5 151659 157445 D Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively; Myo1p 151659..157445 Saccharomyces cerevisiae 856418 NP_011889.1 CDS MAS2 NC_001140.5 157737 159185 R Mas2p; Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins complement(157737..159185) Saccharomyces cerevisiae 856419 NP_011890.1 CDS THR1 NC_001140.5 159431 160504 D Thr1p; Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway 159431..160504 Saccharomyces cerevisiae 856420 NP_011891.1 CDS PPA1 NC_001140.5 160837 161478 D Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain; Ppa1p 160837..161478 Saccharomyces cerevisiae 856421 NP_011892.1 CDS RPN1 NC_001140.5 161723 164704 R Rpn1p; Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions complement(161723..164704) Saccharomyces cerevisiae 856422 NP_011893.1 CDS DAP2 NC_001140.5 164971 167427 R Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p; Dap2p complement(164971..167427) Saccharomyces cerevisiae 856423 NP_011894.1 CDS YHI9 NC_001140.5 167670 168554 R Yhi9p; Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production complement(167670..168554) Saccharomyces cerevisiae 856424 NP_011895.1 CDS SLT2 NC_001140.5 168883 170337 R Slt2p; Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway complement(168883..170337) Saccharomyces cerevisiae 856425 NP_011896.1 CDS RRM3 NC_001140.5 170792 172963 R Rrm3p; DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p complement(170792..172963) Saccharomyces cerevisiae 856426 NP_011897.1 CDS YHR032W NC_001140.5 173337 175082 D Yhr032wp; Putative protein of unknown function; putative substrate of the cAMP-dependent protein kinase (PKA) 173337..175082 Saccharomyces cerevisiae 856427 NP_011898.1 CDS YHR033W NC_001140.5 175541 176812 D Yhr033wp; Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm 175541..176812 Saccharomyces cerevisiae 856428 NP_011899.1 CDS PIH1 NC_001140.5 176958 177992 R Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, Rvb2p) and two rRNA processing factors (Rrp43p, Nop58p); Pih1p complement(176958..177992) Saccharomyces cerevisiae 856429 NP_011900.1 CDS YHR035W NC_001140.5 178212 180104 D Yhr035wp; Putative protein of unknown function; not an essential gene 178212..180104 Saccharomyces cerevisiae 856430 NP_011901.1 CDS BRL1 NC_001140.5 180338 181753 D Essential nuclear envelope integral membrane protein identified as a suppressor of a cold-sensitive mutant of CRM1, a karyopherin; homologous to and interacts with Brr6p, which is a nuclear envelope protein involved in nuclear export; Brl1p 180338..181753 Saccharomyces cerevisiae 856431 NP_011902.1 CDS PUT2 NC_001140.5 181970 183697 D Put2p; Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism 181970..183697 Saccharomyces cerevisiae 856432 NP_011903.1 CDS RRF1 NC_001140.5 184059 184751 D Rrf1p; Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria 184059..184751 Saccharomyces cerevisiae 856433 NP_011904.1 CDS MSC7 NC_001140.5 184868 186802 R Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids; Msc7p complement(184868..186802) Saccharomyces cerevisiae 856434 NP_011905.1 CDS VMA10 NC_001140.5 187166 187672 R Vma10p; Vacuolar H+ ATPase subunit G of the catalytic (V1) sector, involved in vacuolar acidification complement(join(187166..187507,187670..187672)) Saccharomyces cerevisiae 856435 NP_011906.1 CDS BCD1 NC_001140.5 187917 189017 D Essential protein required for the accumulation of box C/D snoRNA; Bcd1p 187917..189017 Saccharomyces cerevisiae 856436 NP_011907.1 CDS SRB2 NC_001140.5 189124 189857 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance; Srb2p complement(join(189124..189742,189844..189857)) Saccharomyces cerevisiae 856437 NP_011908.1 CDS NCP1 NC_001140.5 190536 192611 D Ncp1p; NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p 190536..192611 Saccharomyces cerevisiae 856438 NP_011909.1 CDS DOG2 NC_001140.5 192798 193538 R Dog2p; 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed complement(192798..193538) Saccharomyces cerevisiae 856439 NP_011910.1 CDS DOG1 NC_001140.5 194061 194801 R Dog1p; 2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified complement(194061..194801) Saccharomyces cerevisiae 856440 NP_011911.1 CDS YHR045W NC_001140.5 195544 197226 D Yhr045wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum 195544..197226 Saccharomyces cerevisiae 856441 NP_011912.1 CDS INM1 NC_001140.5 197391 198278 R Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate; Inm1p complement(197391..198278) Saccharomyces cerevisiae 856442 NP_011913.1 CDS AAP1 NC_001140.5 198733 201303 R Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation; Aap1p complement(198733..201303) Saccharomyces cerevisiae 856443 NP_011914.1 CDS YHK8 NC_001140.5 204600 206144 D Yhk8p; Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles 204600..206144 Saccharomyces cerevisiae 856444 NP_011915.1 CDS FSH1 NC_001140.5 206455 207186 D Serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to Fsh2p and Fsh3p; Fsh1p 206455..207186 Saccharomyces cerevisiae 856445 NP_011917.1 CDS SMF2 NC_001140.5 207648 209297 D Smf2p; Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins 207648..209297 Saccharomyces cerevisiae 856447 NP_878088.1 CDS YHR050W-A NC_001140.5 209469 209639 D Yhr050w-ap; Protein of unknown function; identified by expression profiling and mass spectrometry 209469..209639 Saccharomyces cerevisiae 1466528 NP_011918.1 CDS COX6 NC_001140.5 209699 210145 D Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels; Cox6p 209699..210145 Saccharomyces cerevisiae 856448 NP_011919.1 CDS CIC1 NC_001140.5 210842 211972 D Essential protein that interacts with proteasome components and has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles; Cic1p 210842..211972 Saccharomyces cerevisiae 856449 NP_011920.1 CDS CUP1-1 NC_001140.5 212537 212722 R Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; Cup1-1p complement(212537..212722) Saccharomyces cerevisiae 856450 NP_011921.1 CDS YHR054C NC_001140.5 213187 214251 R Yhr054cp; Putative protein of unknown function complement(213187..214251) Saccharomyces cerevisiae 856451 NP_011922.1 CDS CUP1-2 NC_001140.5 214535 214720 R Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; Cup1-2p complement(214535..214720) Saccharomyces cerevisiae 856452 NP_011923.2 CDS RSC30 NC_001140.5 215185 217836 R Rsc30p; Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc3p; null mutants are osmosensitive complement(215185..217836) Saccharomyces cerevisiae 856453 NP_011924.1 CDS CPR2 NC_001140.5 218228 218845 R Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; Cpr2p complement(218228..218845) Saccharomyces cerevisiae 856454 NP_011925.1 CDS MED6 NC_001140.5 218999 219886 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med6p complement(218999..219886) Saccharomyces cerevisiae 856455 NP_011926.1 CDS FYV4 NC_001140.5 220110 220502 D Protein of unknown function, required for survival upon exposure to K1 killer toxin; Fyv4p 220110..220502 Saccharomyces cerevisiae 856456 NP_011927.1 CDS VMA22 NC_001140.5 220727 221272 D Vma22p; Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) 220727..221272 Saccharomyces cerevisiae 856457 NP_011928.1 CDS GIC1 NC_001140.5 221536 222480 R Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; Gic1p complement(221536..222480) Saccharomyces cerevisiae 856458 NP_011929.1 CDS RPP1 NC_001140.5 222879 223760 R Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Rpp1p complement(222879..223760) Saccharomyces cerevisiae 856459 NP_011930.1 CDS PAN5 NC_001140.5 224032 225171 R Pan5p; 2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE complement(224032..225171) Saccharomyces cerevisiae 856460 NP_011931.2 CDS SSZ1 NC_001140.5 225527 227143 R Ssz1p; Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP complement(225527..227143) Saccharomyces cerevisiae 856461 NP_011932.2 CDS RRP3 NC_001140.5 227534 229039 R Rrp3p; Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity complement(227534..229039) Saccharomyces cerevisiae 856462 NP_011933.1 CDS SSF1 NC_001140.5 229337 230698 D Ssf1p; Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family 229337..230698 Saccharomyces cerevisiae 856463 NP_011934.1 CDS HTD2 NC_001140.5 230972 231814 D Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology; Htd2p 230972..231814 Saccharomyces cerevisiae 856464 NP_011935.1 CDS DYS1 NC_001140.5 232135 233298 D Dys1p; Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric 232135..233298 Saccharomyces cerevisiae 856465 NP_011936.1 CDS RRP4 NC_001140.5 233581 234660 R Rrp4p; 3'-5' exoribonuclease involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp43p and Dis3p complement(233581..234660) Saccharomyces cerevisiae 856466 NP_011937.1 CDS TRM5 NC_001140.5 234883 236382 D tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya; Trm5p 234883..236382 Saccharomyces cerevisiae 856467 NP_011938.1 CDS PCL5 NC_001140.5 237006 237695 D Pcl5p; Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity 237006..237695 Saccharomyces cerevisiae 856468 NP_011939.1 CDS ERG7 NC_001140.5 239100 241295 D Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis; Erg7p 239100..241295 Saccharomyces cerevisiae 856470 NP_058135.1 CDS NOP10 NC_001140.5 241666 241842 D Nop10p; Constituent of small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs, which are required for pseudouridylation and processing of pre-18S rRNA 241666..241842 Saccharomyces cerevisiae 856471 NP_011940.1 CDS OSH3 NC_001140.5 242584 245574 D Osh3p; Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability 242584..245574 Saccharomyces cerevisiae 856472 NP_011941.1 CDS QNS1 NC_001140.5 246195 248339 D Qns1p; Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide 246195..248339 Saccharomyces cerevisiae 856473 NP_011942.1 CDS PPE1 NC_001140.5 248441 249643 R Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein; Ppe1p complement(248441..249643) Saccharomyces cerevisiae 856474 NP_011943.2 CDS PTC7 NC_001140.5 251103 252227 D Ptc7p; Mitochondrially localized type 2C protein phosphatase; expression induced by growth on ethanol and by sustained osmotic stress; possible role in carbon source utilization in low oxygen environments join(251103..251157,251251..252227) Saccharomyces cerevisiae 856475 NP_011944.2 CDS NMD2 NC_001140.5 252376 255758 R Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance; Nmd2p complement(join(252376..255639,255753..255758)) Saccharomyces cerevisiae 856476 NP_011945.1 CDS YHR078W NC_001140.5 256362 258020 D Yhr078wp; High osmolarity-regulated gene of unknown function 256362..258020 Saccharomyces cerevisiae 856477 NP_011946.1 CDS IRE1 NC_001140.5 258246 261593 R Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress; Ire1p complement(258246..261593) Saccharomyces cerevisiae 856478 NP_011947.2 CDS SAE3 NC_001140.5 262194 262555 R Sae3p; Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p complement(join(262194..262356,262443..262555)) Saccharomyces cerevisiae 856479 NP_011948.1 CDS YHR080C NC_001140.5 262803 266840 R Yhr080cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(262803..266840) Saccharomyces cerevisiae 856480 NP_011949.1 CDS LRP1 NC_001140.5 267540 268094 D Substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs; required for telomere length maintenance; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination; Lrp1p 267540..268094 Saccharomyces cerevisiae 856481 NP_011950.1 CDS KSP1 NC_001140.5 268461 271550 R Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation; Ksp1p complement(268461..271550) Saccharomyces cerevisiae 856482 NP_011951.1 CDS SAM35 NC_001140.5 272629 273618 D Sam35p; Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane 272629..273618 Saccharomyces cerevisiae 856483 NP_011952.1 CDS STE12 NC_001140.5 274176 276242 D Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth; Ste12p 274176..276242 Saccharomyces cerevisiae 856484 NP_011953.1 CDS IPI1 NC_001140.5 276766 277770 D Ipi1p; Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles 276766..277770 Saccharomyces cerevisiae 856485 NP_011954.1 CDS NAM8 NC_001140.5 278155 279726 D RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA; Nam8p 278155..279726 Saccharomyces cerevisiae 856486 NP_878089.1 CDS YHR086W-A NC_001140.5 280233 280394 D Yhr086w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 280233..280394 Saccharomyces cerevisiae 1466529 NP_011955.1 CDS YHR087W NC_001140.5 280822 281157 D Yhr087wp; Protein of unknown function involved in RNA metabolism; this single domain protein has structural similarity to the N-terminal domain of SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1/YLR022C) 280822..281157 Saccharomyces cerevisiae 856487 NP_011956.1 CDS RPF1 NC_001140.5 281497 282384 D Rpf1p; Nucleolar protein involved in the assembly of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA 281497..282384 Saccharomyces cerevisiae 856488 NP_011957.1 CDS GAR1 NC_001140.5 282683 283300 R Protein component of the H/ACA snoRNP pseudouridylase complex, involved in the modification and cleavage of the 18S pre-rRNA; Gar1p complement(282683..283300) Saccharomyces cerevisiae 856489 NP_011958.1 CDS YNG2 NC_001140.5 283779 284627 R Yng2p; Subunit of the NuA4 histone acetyltransferase complex that acetylates histone H4 and H2A; has similarity to the human tumor suppressor ING1 complement(283779..284627) Saccharomyces cerevisiae 856490 NP_011959.1 CDS MSR1 NC_001140.5 284841 286772 R Msr1p; Mitochondrial arginyl-tRNA synthetase complement(284841..286772) Saccharomyces cerevisiae 856491 NP_011960.1 CDS HXT4 NC_001140.5 287132 288814 R Hxt4p; High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose complement(287132..288814) Saccharomyces cerevisiae 856492 NP_011962.1 CDS HXT1 NC_001140.5 290916 292628 R Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; Hxt1p complement(290916..292628) Saccharomyces cerevisiae 856494 NP_011964.1 CDS HXT5 NC_001140.5 294672 296450 R Hxt5p; Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs complement(294672..296450) Saccharomyces cerevisiae 856496 NP_011965.1 CDS YHR097C NC_001140.5 297388 298612 R Yhr097cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus complement(join(297388..298363,298488..298612)) Saccharomyces cerevisiae 856497 NP_011966.1 CDS SFB3 NC_001140.5 299148 301937 R Sfb3p; Member of the Sec24p family; forms a complex, with Sec23p, that is involved in sorting of Pma1p into COPII vesicles; peripheral ER membrane protein; potential Cdc28p substrate complement(299148..301937) Saccharomyces cerevisiae 856498 NP_011967.1 CDS TRA1 NC_001140.5 302764 313998 D Tra1p; Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation 302764..313998 Saccharomyces cerevisiae 856499 NP_011968.1 CDS YHR100C NC_001140.5 314119 314676 R Yhr100cp; Putative protein of unknown function, required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(314119..314676) Saccharomyces cerevisiae 856500 NP_011969.1 CDS BIG1 NC_001140.5 314877 315971 R Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan; Big1p complement(join(314877..315774,315862..315971)) Saccharomyces cerevisiae 856501 NP_011970.1 CDS KIC1 NC_001140.5 316575 319817 D Protein kinase of the PAK/Ste20 kinase family, required for cell integrity possibly through regulating 1,6-beta-glucan levels in the wall; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; Kic1p 316575..319817 Saccharomyces cerevisiae 856502 NP_011971.1 CDS SBE22 NC_001140.5 320417 322975 D Sbe22p; Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth 320417..322975 Saccharomyces cerevisiae 856503 NP_011972.1 CDS GRE3 NC_001140.5 323412 324395 D Gre3p; Aldose reductase involved in methylglyoxal, d-xylose and arabinose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway 323412..324395 Saccharomyces cerevisiae 856504 NP_011973.1 CDS YPT35 NC_001140.5 324769 325413 D Ypt35p; Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport 324769..325413 Saccharomyces cerevisiae 856505 NP_011974.1 CDS TRR2 NC_001140.5 325601 326629 D Trr2p; Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD 325601..326629 Saccharomyces cerevisiae 856506 NP_011975.1 CDS CDC12 NC_001140.5 326816 328039 R Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM; Cdc12p complement(326816..328039) Saccharomyces cerevisiae 856507 NP_011976.1 CDS GGA2 NC_001140.5 328306 330063 D Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; Gga2p 328306..330063 Saccharomyces cerevisiae 856508 NP_011977.1 CDS CTM1 NC_001140.5 330313 332070 D Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth; Ctm1p 330313..332070 Saccharomyces cerevisiae 856509 NP_011978.1 CDS ERP5 NC_001140.5 332285 332923 D Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; Erp5p 332285..332923 Saccharomyces cerevisiae 856510 NP_011979.1 CDS UBA4 NC_001140.5 333075 334397 D Uba4p; Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response 333075..334397 Saccharomyces cerevisiae 856511 NP_011980.1 CDS YHR112C NC_001140.5 334530 335666 R Yhr112cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(334530..335666) Saccharomyces cerevisiae 856512 NP_011981.1 CDS YHR113W NC_001140.5 336340 337812 D Yhr113wp; Cytoplasmic aspartyl aminopeptidase; cleaves unblocked N-terminal acidic amino acid residues from peptide substrates; forms a 12 subunit homo-oligomeric complex; M18 metalloprotease family member; may interact with ribosomes 336340..337812 Saccharomyces cerevisiae 856513 NP_011982.1 CDS BZZ1 NC_001140.5 338086 339987 D SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins; Bzz1p 338086..339987 Saccharomyces cerevisiae 856514 NP_011983.1 CDS DMA1 NC_001140.5 340112 341362 R Protein involved in regulating spindle position and orientation, functionally redundant with Dma2p; homolog of S. pombe Dma1 and H. sapiens Chfr; Dma1p complement(340112..341362) Saccharomyces cerevisiae 856515 NP_011984.1 CDS COX23 NC_001140.5 341668 342123 D Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; Cox23p 341668..342123 Saccharomyces cerevisiae 856516 NP_011985.1 CDS TOM71 NC_001140.5 342352 344271 D Tom71p; Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins 342352..344271 Saccharomyces cerevisiae 856517 NP_011986.1 CDS ORC6 NC_001140.5 344324 345631 R Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; Orc6p complement(344324..345631) Saccharomyces cerevisiae 856518 NP_011987.1 CDS SET1 NC_001140.5 346046 349288 D Set1p; Histone methyltransferase, subunit of the COMPASS (Set1C) complex which methylates histone H3 on lysine 4; required in transcriptional silencing near telomeres and at the silent mating type loci; contains a SET domain 346046..349288 Saccharomyces cerevisiae 856519 NP_011988.1 CDS MSH1 NC_001140.5 349577 352456 D Msh1p; DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis 349577..352456 Saccharomyces cerevisiae 856520 NP_011989.1 CDS LSM12 NC_001140.5 352759 353322 D Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; Lsm12p 352759..353322 Saccharomyces cerevisiae 856521 NP_011990.1 CDS YHR122W NC_001140.5 353628 354323 D Yhr122wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; may be involved in transcription; YHR122W is an essential gene 353628..354323 Saccharomyces cerevisiae 856522 NP_011991.1 CDS EPT1 NC_001140.5 354818 356084 D sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; Ept1p join(354818..354867,354959..356084) Saccharomyces cerevisiae 856523 NP_011992.1 CDS NDT80 NC_001140.5 356564 358447 D Ndt80p; Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) 356564..358447 Saccharomyces cerevisiae 856524 NP_011994.1 CDS YHR126C NC_001140.5 359705 360184 R Yhr126cp; Putative protein of unknown function; transcription dependent upon Azf1p complement(359705..360184) Saccharomyces cerevisiae 856527 NP_011995.1 CDS YHR127W NC_001140.5 360916 361647 D Yhr127wp; Protein of unknown function; localizes to the nucleus 360916..361647 Saccharomyces cerevisiae 856528 NP_011996.2 CDS FUR1 NC_001140.5 362118 362768 D Uracil phosphoribosyltransferase, synthesizes UMP from uracil; involved in the pyrimidine salvage pathway; Fur1p 362118..362768 Saccharomyces cerevisiae 856529 NP_011997.1 CDS ARP1 NC_001140.5 363002 364156 R Arp1p; Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin complement(363002..364156) Saccharomyces cerevisiae 856530 NP_011999.2 CDS YHR131C NC_001140.5 365343 367895 R Yhr131cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(365343..367895) Saccharomyces cerevisiae 856532 NP_012000.1 CDS ECM14 NC_001140.5 368505 369797 R Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly; Ecm14p complement(368505..369797) Saccharomyces cerevisiae 856533 NP_036194.1 CDS IGO2 NC_001140.5 370057 370452 D Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; Igo2p 370057..370452 Saccharomyces cerevisiae 856534 NP_012001.1 CDS NSG1 NC_001140.5 370725 371600 R Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; Nsg1p complement(370725..371600) Saccharomyces cerevisiae 856535 NP_012002.1 CDS WSS1 NC_001140.5 371752 372561 D Wss1p; Sumoylated protein of unknown function, identified based on genetic interactions with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response, processing stalled or collapsed replication forks 371752..372561 Saccharomyces cerevisiae 856536 NP_012003.1 CDS YCK1 NC_001140.5 372697 374313 R Yck1p; Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p complement(372697..374313) Saccharomyces cerevisiae 856537 NP_012004.1 CDS SPL2 NC_001140.5 374657 375103 R Spl2p; Protein with similarity to cyclin-dependent kinase inhibitors, overproduction suppresses a plc1 null mutation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(374657..375103) Saccharomyces cerevisiae 856538 NP_012005.1 CDS ARO9 NC_001140.5 375712 377253 D Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism; Aro9p 375712..377253 Saccharomyces cerevisiae 856539 NP_012006.1 CDS YHR138C NC_001140.5 377358 377702 R Yhr138cp; Putative protein of unknown function; has similarity to Pbi2p; double null mutant lacking Pbi2p and Yhr138p exhibits highly fragmented vacuoles complement(377358..377702) Saccharomyces cerevisiae 856540 NP_012007.1 CDS SPS100 NC_001140.5 378222 379202 R Sps100p; Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall complement(378222..379202) Saccharomyces cerevisiae 856541 NP_012009.1 CDS YHR140W NC_001140.5 380575 381294 D Yhr140wp; Putative integral membrane protein of unknown function 380575..381294 Saccharomyces cerevisiae 856543 NP_012010.1 CDS RPL42B NC_001140.5 381993 382754 R Rpl42bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl42Ap and has similarity to rat L44; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus complement(join(381993..382309,382751..382754)) Saccharomyces cerevisiae 856544 NP_012011.1 CDS CHS7 NC_001140.5 383541 384491 D Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER; Chs7p 383541..384491 Saccharomyces cerevisiae 856545 NP_012012.1 CDS DSE2 NC_001140.5 385513 386490 D Dse2p; Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP 385513..386490 Saccharomyces cerevisiae 856546 NP_012013.1 CDS RPC10 NC_001140.5 387236 387448 D Rpc10p; RNA polymerase subunit, found in RNA polymerase complexes I, II, and III 387236..387448 Saccharomyces cerevisiae 856547 NP_012014.1 CDS DCD1 NC_001140.5 387791 388729 R Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT cell cycle regulated; Dcd1p complement(387791..388729) Saccharomyces cerevisiae 856548 NP_012016.1 CDS CRP1 NC_001140.5 390303 391700 D Protein that binds to cruciform DNA structures; Crp1p 390303..391700 Saccharomyces cerevisiae 856551 NP_012017.1 CDS MRPL6 NC_001140.5 392642 393286 R Mrpl6p; Mitochondrial ribosomal protein of the large subunit complement(392642..393286) Saccharomyces cerevisiae 856552 NP_012018.1 CDS IMP3 NC_001140.5 393537 394088 D Imp3p; Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA 393537..394088 Saccharomyces cerevisiae 856553 NP_012019.1 CDS SKG6 NC_001140.5 394458 396662 R Skg6p; Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p complement(394458..396662) Saccharomyces cerevisiae 856554 NP_012020.1 CDS PEX28 NC_001140.5 397254 398993 D Pex28p; Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p 397254..398993 Saccharomyces cerevisiae 856555 NP_012021.1 CDS YHR151C NC_001140.5 399271 400851 R Yhr151cp; Putative protein of unknown function complement(399271..400851) Saccharomyces cerevisiae 856556 NP_012022.1 CDS SPO12 NC_001140.5 401437 401958 D Spo12p; Nucleolar protein of unknown function, positive regulator of exit from mitosis; involved in regulating the release of Cdc14p from the nucleolus in early anaphase; proposed to play similar role in meiosis 401437..401958 Saccharomyces cerevisiae 856557 NP_012023.1 CDS SPO16 NC_001140.5 402089 402685 R Spo16p; Protein of unknown function, required for spore formation complement(402089..402685) Saccharomyces cerevisiae 856558 NP_012024.1 CDS RTT107 NC_001140.5 402969 406181 D Rtt107p; Protein implicated in Mms22-dependent DNA repair during S phase, DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition 402969..406181 Saccharomyces cerevisiae 856559 NP_012025.1 CDS YSP1 NC_001140.5 407106 410792 D Ysp1p; Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone 407106..410792 Saccharomyces cerevisiae 856560 NP_012026.1 CDS LIN1 NC_001140.5 411387 412409 R Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication; Lin1p complement(411387..412409) Saccharomyces cerevisiae 856561 NP_012027.1 CDS REC104 NC_001140.5 412910 413458 D Rec104p; Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination 412910..413458 Saccharomyces cerevisiae 856562 NP_012028.1 CDS KEL1 NC_001140.5 413688 417182 R Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; Kel1p complement(413688..417182) Saccharomyces cerevisiae 856563 NP_012029.1 CDS YHR159W NC_001140.5 417552 419066 D Yhr159wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate 417552..419066 Saccharomyces cerevisiae 856564 NP_012030.1 CDS PEX18 NC_001140.5 419224 420075 R Pex18p; Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p complement(419224..420075) Saccharomyces cerevisiae 856565 NP_012031.1 CDS YAP1801 NC_001140.5 420376 422289 R Yap1801p; Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the AP180 protein family complement(420376..422289) Saccharomyces cerevisiae 856566 NP_012032.1 CDS YHR162W NC_001140.5 423075 423464 D Yhr162wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion 423075..423464 Saccharomyces cerevisiae 856567 NP_012033.2 CDS SOL3 NC_001140.5 423726 424475 D Sol3p; 6-phosphogluconolactonase, catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p 423726..424475 Saccharomyces cerevisiae 856568 NP_012034.1 CDS DNA2 NC_001140.5 424612 429180 R Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate; Dna2p complement(424612..429180) Saccharomyces cerevisiae 856569 NP_012035.1 CDS PRP8 NC_001140.5 429709 436950 R Prp8p; Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutations of human Prp8 cause retinitis pigmentosa complement(429709..436950) Saccharomyces cerevisiae 856570 NP_012036.1 CDS CDC23 NC_001140.5 437172 439052 R Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Cdc23p complement(437172..439052) Saccharomyces cerevisiae 856571 NP_012037.1 CDS THP2 NC_001140.5 439344 440129 D Thp2p; Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance 439344..440129 Saccharomyces cerevisiae 856572 NP_012038.1 CDS MTG2 NC_001140.5 440379 441878 D Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly; Mtg2p 440379..441878 Saccharomyces cerevisiae 856573 NP_012039.1 CDS DBP8 NC_001140.5 442182 443477 D Putative ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 40S ribosomal subunit; Dbp8p 442182..443477 Saccharomyces cerevisiae 856574 NP_012040.1 CDS NMD3 NC_001140.5 443829 445385 D Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex; Nmd3p 443829..445385 Saccharomyces cerevisiae 856575 NP_012041.1 CDS ATG7 NC_001140.5 445713 447605 D Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation; Atg7p 445713..447605 Saccharomyces cerevisiae 856576 NP_012042.1 CDS SPC97 NC_001140.5 448335 450806 D Spc97p; Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque 448335..450806 Saccharomyces cerevisiae 856577 NP_012044.1 CDS ENO2 NC_001140.5 451327 452640 D Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose; Eno2p 451327..452640 Saccharomyces cerevisiae 856579 NP_012045.1 CDS CTR2 NC_001140.5 452872 453441 D Putative low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation; Ctr2p 452872..453441 Saccharomyces cerevisiae 856580 NP_878090.1 CDS YHR175W-A NC_001140.5 453558 453707 D Yhr175w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 453558..453707 Saccharomyces cerevisiae 1466530 NP_012046.2 CDS FMO1 NC_001140.5 454229 455527 D Fmo1p; Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins 454229..455527 Saccharomyces cerevisiae 856581 NP_012047.1 CDS YHR177W NC_001140.5 456591 457952 D Yhr177wp; Putative protein of unknown function 456591..457952 Saccharomyces cerevisiae 856582 NP_012048.1 CDS STB5 NC_001140.5 459299 461530 D Stb5p; Activator of multidrug resistance genes, forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a PDRE (pleotropic drug resistance element) in vitro; binds Sin3p in a two-hybrid assay 459299..461530 Saccharomyces cerevisiae 856583 NP_012049.1 CDS OYE2 NC_001140.5 462502 463704 D Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism; Oye2p 462502..463704 Saccharomyces cerevisiae 856584 NP_012051.1 CDS SVP26 NC_001140.5 467228 467914 D Svp26p; Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment 467228..467914 Saccharomyces cerevisiae 856587 NP_012052.1 CDS YHR182W NC_001140.5 468219 470576 D Yhr182wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm 468219..470576 Saccharomyces cerevisiae 856588 NP_012053.1 CDS GND1 NC_001140.5 470960 472429 D Gnd1p; 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress 470960..472429 Saccharomyces cerevisiae 856589 NP_012054.1 CDS SSP1 NC_001140.5 472744 474459 D Ssp1p; Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis 472744..474459 Saccharomyces cerevisiae 856590 NP_012055.1 CDS PFS1 NC_001140.5 474627 475340 R Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation; Pfs1p complement(474627..475340) Saccharomyces cerevisiae 856591 NP_012056.1 CDS KOG1 NC_001140.5 475999 480672 R Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors; Kog1p complement(475999..480672) Saccharomyces cerevisiae 856593 NP_012057.1 CDS IKI1 NC_001140.5 480990 481919 D Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin; Iki1p 480990..481919 Saccharomyces cerevisiae 856594 NP_012058.1 CDS GPI16 NC_001140.5 482005 483837 R Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog; Gpi16p complement(482005..483837) Saccharomyces cerevisiae 856595 NP_012059.1 CDS PTH1 NC_001140.5 484028 484600 D Pth1p; One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth and for mitochondrial respiration 484028..484600 Saccharomyces cerevisiae 856596 NP_012060.1 CDS ERG9 NC_001140.5 484845 486179 D Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway; Erg9p 484845..486179 Saccharomyces cerevisiae 856597 NP_012061.1 CDS CTF8 NC_001140.5 486230 486631 R Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; Ctf8p complement(486230..486631) Saccharomyces cerevisiae 856598 NP_012062.1 CDS YHR192W NC_001140.5 486826 487662 D Yhr192wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS 486826..487662 Saccharomyces cerevisiae 856599 NP_012063.1 CDS EGD2 NC_001140.5 487712 488236 R Egd2p; Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes complement(487712..488236) Saccharomyces cerevisiae 856600 NP_012064.1 CDS MDM31 NC_001140.5 488657 490396 D Mitochondrial inner membrane protein with similarity to Mdm32p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; Mdm31p 488657..490396 Saccharomyces cerevisiae 856601 NP_012065.1 CDS NVJ1 NC_001140.5 490747 491712 D Nvj1p; Nuclear envelope protein that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) 490747..491712 Saccharomyces cerevisiae 856602 NP_012066.1 CDS UTP9 NC_001140.5 491931 493658 D Utp9p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA 491931..493658 Saccharomyces cerevisiae 856603 NP_012067.1 CDS RIX1 NC_001140.5 493896 496187 D Rix1p; Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles 493896..496187 Saccharomyces cerevisiae 856604 NP_012068.1 CDS AIM18 NC_001140.5 496315 497280 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim18p complement(496315..497280) Saccharomyces cerevisiae 856605 NP_012069.1 CDS AIM46 NC_001140.5 497490 498422 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim46p complement(497490..498422) Saccharomyces cerevisiae 856606 NP_976247.1 CDS YHR199C-A NC_001140.5 498571 498859 R Yhr199c-ap; Putative protein of unknown function; transcribed sequence appears to contain an intron complement(join(498571..498719,498787..498859)) Saccharomyces cerevisiae 2746858 NP_012070.1 CDS RPN10 NC_001140.5 499079 499885 D Rpn10p; Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein 499079..499885 Saccharomyces cerevisiae 856607 NP_012071.1 CDS PPX1 NC_001140.5 499950 501143 R Ppx1p; Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix complement(499950..501143) Saccharomyces cerevisiae 856608 NP_012072.1 CDS YHR202W NC_001140.5 502388 504196 D Yhr202wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization 502388..504196 Saccharomyces cerevisiae 856609 NP_012073.1 CDS RPS4B NC_001140.5 504476 505530 R Rps4bp; Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein complement(join(504476..505247,505517..505530)) Saccharomyces cerevisiae 856610 NP_012074.1 CDS MNL1 NC_001140.5 506319 508709 D Mnl1p; Alpha mannosidase-like protein of the endoplasmic reticulum required for degradation of glycoproteins but not for processing of N-linked oligosaccharides 506319..508709 Saccharomyces cerevisiae 856611 NP_012075.1 CDS SCH9 NC_001140.5 509363 511837 D Sch9p; Protein kinase involved in transcriptional activation of osmostress-responsive genes; regulates G1 progression, cAPK activity, nitrogen activation of the FGM pathway; involved in life span regulation; homologous to mammalian Akt/PKB 509363..511837 Saccharomyces cerevisiae 856612 NP_012076.1 CDS SKN7 NC_001140.5 512732 514600 D Skn7p; Nuclear response regulator and transcription factor, part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation 512732..514600 Saccharomyces cerevisiae 856613 NP_012077.1 CDS SET5 NC_001140.5 514905 516485 R Zinc-finger protein of unknown function, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus; Set5p complement(514905..516485) Saccharomyces cerevisiae 856614 NP_012078.1 CDS BAT1 NC_001140.5 517532 518713 D Mitochondrial branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase; Bat1p 517532..518713 Saccharomyces cerevisiae 856615 NP_012079.1 CDS CRG1 NC_001140.5 519437 520312 D Putative S-adenosylmethionine-dependent methyltransferase; mediates cantharidin resistance; Crg1p 519437..520312 Saccharomyces cerevisiae 856616 NP_012080.1 CDS YHR210C NC_001140.5 520712 521737 R Yhr210cp; Putative protein of unknown function; non-essential gene; highly expressed under anaeorbic conditions; sequence similarity to aldose 1-epimerases such as GAL10 complement(520712..521737) Saccharomyces cerevisiae 856617 NP_012081.1 CDS FLO5 NC_001140.5 525392 528619 D Lectin-like cell wall protein (flocculin) involved in flocculation, binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; similar to Flo1p; Flo5p 525392..528619 Saccharomyces cerevisiae 856618 NP_878091.1 CDS YHR212W-A NC_001140.5 538742 538945 D Yhr212w-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 538742..538945 Saccharomyces cerevisiae 1466531 NP_012083.1 CDS YHR213W NC_001140.5 539151 539747 D Yhr213wp; Putative protein of unknown function 539151..539747 Saccharomyces cerevisiae 856620 NP_878092.1 CDS YHR213W-A NC_001140.5 540549 540782 D Yhr213w-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 540549..540782 Saccharomyces cerevisiae 1466532 NP_878093.1 CDS YHR213W-B NC_001140.5 540800 541099 D Yhr213w-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 540800..541099 Saccharomyces cerevisiae 1466533 NP_012084.1 CDS YHR214W NC_001140.5 541651 542262 D Yhr214wp; Putative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein 541651..542262 Saccharomyces cerevisiae 856621 NP_012086.1 CDS YHR214C-B NC_001140.5 543964 549346 R Yhr214c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(543964..547929,547931..549346)) Saccharomyces cerevisiae 856623 NP_036195.1 CDS YHR214C-C NC_001140.5 547910 549346 R Yhr214c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(547910..549346) Saccharomyces cerevisiae 856624 NP_878094.1 CDS YHR214C-D NC_001140.5 550648 550941 R Yhr214c-dp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(550648..550941) Saccharomyces cerevisiae 1466534 NP_878095.1 CDS YHR214C-E NC_001140.5 551200 551499 R Yhr214c-ep; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(551200..551499) Saccharomyces cerevisiae 1466535 NP_012087.1 CDS PHO12 NC_001140.5 552099 553502 D Pho12p; One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; nearly identical to Pho11p; upregulated by phosphate starvation 552099..553502 Saccharomyces cerevisiae 856625 NP_012088.1 CDS IMD2 NC_001140.5 554396 555967 D Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; Imd2p 554396..555967 Saccharomyces cerevisiae 856626 NP_012090.1 CDS YHR218W NC_001140.5 558014 559924 D Yhr218wp; Helicase-like protein encoded within the telomeric Y' element join(558014..558615,558715..559924) Saccharomyces cerevisiae 856628 NP_012091.1 CDS YHR219W NC_001140.5 560173 562047 D Yhr219wp; Putative protein of unknown function with similarity to helicases; located in the telomere region on the right arm of chromosome VIII 560173..562047 Saccharomyces cerevisiae 856629 NP_012311.1 CDS YJL225C NC_001142.7 466 6130 R Yjl225cp; Putative protein of unknown function; similarity to DNA helicases that are also encoded within subtelomeric Y' elements and induced in telomerase deficient survivors complement(join(466..4581,4970..6130)) Saccharomyces cerevisiae 853231 NP_012312.1 CDS PAU1 NC_001142.7 8776 9138 R Pau1p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme complement(8776..9138) Saccharomyces cerevisiae 853232 NP_012313.1 CDS VTH2 NC_001142.7 11475 16124 D Vth2p; Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting 11475..16124 Saccharomyces cerevisiae 853233 NP_012314.1 CDS FSP2 NC_001142.7 16767 18536 R Protein of unknown function, expression is induced during nitrogen limitation; Fsp2p complement(16767..18536) Saccharomyces cerevisiae 853235 NP_012316.1 CDS HXT9 NC_001142.7 19497 21200 D Putative hexose transporter that is nearly identical to Hxt11p, has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p; Hxt9p 19497..21200 Saccharomyces cerevisiae 853236 NP_012317.1 CDS YJL218W NC_001142.7 21973 22563 D Yjl218wp; Putative protein of unknown function, similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene 21973..22563 Saccharomyces cerevisiae 853237 NP_012318.1 CDS YJL217W NC_001142.7 23133 23729 D Yjl217wp; Cytoplasmic protein of unknown function; expression induced by calcium shortage and via the copper sensing transciption factor Mac1p during conditons of copper deficiency; mRNA is cell cycle regulated, peaking in G1 phase 23133..23729 Saccharomyces cerevisiae 853238 NP_012319.1 CDS YJL216C NC_001142.7 24341 26086 R Yjl216cp; Protein of unknown function, similar to alpha-D-glucosidases; transcriptionally activated by both Pdr8p and Yrm1p, along with transporters and other genes involved in the pleiotropic drug resistance (PDR) phenomenon complement(24341..26086) Saccharomyces cerevisiae 853214 NP_012321.1 CDS HXT8 NC_001142.7 26887 28596 D Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose; Hxt8p 26887..28596 Saccharomyces cerevisiae 853216 NP_012322.1 CDS YJL213W NC_001142.7 32163 33158 D Yjl213wp; Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p 32163..33158 Saccharomyces cerevisiae 853217 NP_012323.1 CDS OPT1 NC_001142.7 33850 36249 R Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family; Opt1p complement(33850..36249) Saccharomyces cerevisiae 853218 NP_012325.1 CDS PEX2 NC_001142.7 36919 37734 D RING-finger peroxin, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import; Pex2p 36919..37734 Saccharomyces cerevisiae 853219 NP_012326.1 CDS CBP1 NC_001142.7 38005 39969 D Mitochondrial protein that interacts with the 5'-untranslated region of the COB mRNA and has a role in its stability and translation; found in a complex at the inner membrane along with Pet309p; Cbp1p 38005..39969 Saccharomyces cerevisiae 853221 NP_012327.1 CDS NUC1 NC_001142.7 40194 41183 R Nuc1p; Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy complement(40194..41183) Saccharomyces cerevisiae 853222 NP_012328.1 CDS LAA1 NC_001142.7 41389 47433 R Laa1p; AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene complement(41389..47433) Saccharomyces cerevisiae 853223 NP_012329.1 CDS YJL206C NC_001142.7 47659 49935 R Yjl206cp; Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment complement(47659..49935) Saccharomyces cerevisiae 853224 NP_012330.1 CDS NCE101 NC_001142.7 50139 50443 R Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences; Nce101p complement(join(50139..50268,50412..50443)) Saccharomyces cerevisiae 853225 NP_012331.1 CDS RCY1 NC_001142.7 50629 53151 R Rcy1p; F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth complement(50629..53151) Saccharomyces cerevisiae 853226 NP_012332.1 CDS PRP21 NC_001142.7 53341 54183 D Subunit of the SF3a splicing factor complex, required for spliceosome assembly; Prp21p 53341..54183 Saccharomyces cerevisiae 853227 NP_012334.1 CDS ECM25 NC_001142.7 54379 56178 D Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p; Ecm25p 54379..56178 Saccharomyces cerevisiae 853229 NP_012335.1 CDS ACO2 NC_001142.7 56444 58813 R Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol; Aco2p complement(56444..58813) Saccharomyces cerevisiae 853230 NP_012337.1 CDS PHO90 NC_001142.7 60843 63488 D Pho90p; Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth 60843..63488 Saccharomyces cerevisiae 853241 NP_012338.1 CDS UBP12 NC_001142.7 63804 67568 D Ubp12p; Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease present in the nucleus and cytoplasm that cleaves ubiquitin from ubiquitinated proteins 63804..67568 Saccharomyces cerevisiae 853242 NP_012339.1 CDS ELO1 NC_001142.7 67849 68781 R Elongase I, medium-chain acyl elongase, catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; Elo1p complement(67849..68781) Saccharomyces cerevisiae 853243 NP_012341.1 CDS CDC6 NC_001142.7 69337 70878 D Essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; Cdc6p 69337..70878 Saccharomyces cerevisiae 853244 NP_012342.1 CDS YJL193W NC_001142.7 71365 72573 D Yjl193wp; Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect 71365..72573 Saccharomyces cerevisiae 853246 NP_012343.1 CDS SOP4 NC_001142.7 72709 73413 R Sop4p; ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER complement(72709..73413) Saccharomyces cerevisiae 853247 NP_012344.1 CDS RPS14B NC_001142.7 73786 74610 D Rps14bp; Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins join(73786..73795,74204..74610) Saccharomyces cerevisiae 853248 NP_012345.1 CDS RPS22A NC_001142.7 74909 75301 R Rps22ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Bp and has similarity to E. coli S8 and rat S15a ribosomal proteins complement(74909..75301) Saccharomyces cerevisiae 853249 NP_012346.1 CDS RPL39 NC_001142.7 75932 76473 D Rpl39p; Protein component of the large (60S) ribosomal subunit, has similarity to rat L39 ribosomal protein; required for ribosome biogenesis; exhibits genetic interactions with SIS1 and PAB1 join(75932..75937,76324..76473) Saccharomyces cerevisiae 853250 NP_012348.1 CDS SWE1 NC_001142.7 76802 79261 R Swe1p; Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate complement(76802..79261) Saccharomyces cerevisiae 853252 NP_012349.1 CDS MNN5 NC_001142.7 80153 81913 D Mnn5p; Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment 80153..81913 Saccharomyces cerevisiae 853253 NP_012350.1 CDS YJL185C NC_001142.7 82093 82974 R Yjl185cp; Putative protein of unknown function; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene complement(82093..82974) Saccharomyces cerevisiae 853254 NP_012351.1 CDS GON7 NC_001142.7 83446 83817 D Protein proposed to be involved in the modification of N-linked oligosaccharides, osmotic stress response, telomere uncapping and elongation, transcription; component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Bud32p; Gon7p 83446..83817 Saccharomyces cerevisiae 853255 NP_012352.1 CDS MNN11 NC_001142.7 84066 85334 D Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p; Mnn11p 84066..85334 Saccharomyces cerevisiae 853256 NP_012354.1 CDS YJL181W NC_001142.7 85658 87493 D Yjl181wp; Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis 85658..87493 Saccharomyces cerevisiae 853257 NP_012355.1 CDS ATP12 NC_001142.7 87581 88558 R Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency; Atp12p complement(87581..88558) Saccharomyces cerevisiae 853259 NP_012356.1 CDS PFD1 NC_001142.7 88785 89114 D Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; Pfd1p 88785..89114 Saccharomyces cerevisiae 853260 NP_012357.2 CDS ATG27 NC_001142.7 89280 90095 R Type II membrane protein involved in autophagy; binds phosphatidylinositol 3-phosphate, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; Atg27p complement(89280..90095) Saccharomyces cerevisiae 853261 NP_012358.1 CDS RPL17B NC_001142.7 90784 91655 D Rpl17bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similarity to E. coli L22 and rat L17 ribosomal proteins join(90784..91092,91410..91655) Saccharomyces cerevisiae 853262 NP_012359.1 CDS SWI3 NC_001142.7 92051 94528 R Swi3p; Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2 complement(92051..94528) Saccharomyces cerevisiae 853264 NP_012361.1 CDS KRE9 NC_001142.7 95090 95920 D Kre9p; Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects 95090..95920 Saccharomyces cerevisiae 853265 NP_012362.1 CDS RFA3 NC_001142.7 96159 96527 R Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; Rfa3p complement(96159..96527) Saccharomyces cerevisiae 853266 NP_012363.1 CDS CPS1 NC_001142.7 97731 99461 D Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions; Cps1p 97731..99461 Saccharomyces cerevisiae 853267 NP_012364.1 CDS YJL171C NC_001142.7 99698 100888 R Yjl171cp; GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein complement(99698..100888) Saccharomyces cerevisiae 853268 NP_012365.2 CDS ASG7 NC_001142.7 101144 101773 R Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor; Asg7p complement(101144..101773) Saccharomyces cerevisiae 853269 NP_012367.1 CDS SET2 NC_001142.7 102221 104422 R Set2p; Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p complement(102221..104422) Saccharomyces cerevisiae 853271 NP_012368.1 CDS ERG20 NC_001142.7 105008 106066 D Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis; Erg20p 105008..106066 Saccharomyces cerevisiae 853272 NP_012369.1 CDS QCR8 NC_001142.7 106428 106712 D Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p; Qcr8p 106428..106712 Saccharomyces cerevisiae 853273 NP_012370.1 CDS HAL5 NC_001142.7 106888 109455 R Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; Hal5p complement(106888..109455) Saccharomyces cerevisiae 853274 NP_012371.1 CDS TPK1 NC_001142.7 109960 111153 R cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk2p and Tpk3p; Tpk1p complement(109960..111153) Saccharomyces cerevisiae 853275 NP_012372.1 CDS YJL163C NC_001142.7 111662 113329 R Yjl163cp; Putative protein of unknown function complement(111662..113329) Saccharomyces cerevisiae 853276 NP_012373.2 CDS JJJ2 NC_001142.7 113874 115625 R Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein; Jjj2p complement(113874..115625) Saccharomyces cerevisiae 853277 NP_012374.1 CDS FMP33 NC_001142.7 117241 117783 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp33p 117241..117783 Saccharomyces cerevisiae 853279 NP_012375.2 CDS YJL160C NC_001142.7 117958 118821 R Yjl160cp; Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis complement(117958..118821) Saccharomyces cerevisiae 853280 NP_012376.3 CDS HSP150 NC_001142.7 120445 121686 D O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; Hsp150p 120445..121686 Saccharomyces cerevisiae 853281 NP_012377.1 CDS CIS3 NC_001142.7 122261 122944 R Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family; Cis3p complement(122261..122944) Saccharomyces cerevisiae 853282 NP_012378.1 CDS FAR1 NC_001142.7 123832 126324 R Far1p; Cyclin-dependent kinase inhibitor that mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate complement(123832..126324) Saccharomyces cerevisiae 853283 NP_012379.1 CDS SSY5 NC_001142.7 126886 128949 R Ssy5p; Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes complement(126886..128949) Saccharomyces cerevisiae 853285 NP_012380.1 CDS FBP26 NC_001142.7 129282 130640 R Fructose-2,6-bisphosphatase, required for glucose metabolism; Fbp26p complement(129282..130640) Saccharomyces cerevisiae 853286 NP_012381.1 CDS VPS35 NC_001142.7 131098 133932 R Vps35p; Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval complement(131098..133932) Saccharomyces cerevisiae 853287 NP_012382.2 CDS INO1 NC_001142.7 134329 135930 R Inositol 1-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element; Ino1p complement(134329..135930) Saccharomyces cerevisiae 853288 NP_012384.1 CDS SNA3 NC_001142.7 136369 136770 R Sna3p; Integral membrane protein localized to vacuolar intralumenal vesicles, computational analysis of large-scale protein-protein interaction data suggests a possible role in either cell wall synthesis or protein-vacuolar targeting complement(136369..136770) Saccharomyces cerevisiae 853290 NP_012386.1 CDS DAS1 NC_001142.7 137376 139367 D Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C; null mutant suppresses dst1delta sensitivity for 6-azauracil; Das1p 137376..139367 Saccharomyces cerevisiae 853292 NP_012387.1 CDS RPA34 NC_001142.7 140434 141135 D Rpa34p; RNA polymerase I subunit A34.5 140434..141135 Saccharomyces cerevisiae 853293 NP_012388.1 CDS YJL147C NC_001142.7 141416 142564 R Yjl147cp; Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene complement(141416..142564) Saccharomyces cerevisiae 853294 NP_012389.1 CDS IDS2 NC_001142.7 143289 144698 D Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation; Ids2p 143289..144698 Saccharomyces cerevisiae 853295 NP_012390.1 CDS SFH5 NC_001142.7 145157 146041 D Sfh5p; Putative phosphatidylinositol transfer protein (PITP), exhibits phosphatidylinositol- but not phosphatidylcholine-transfer activity, localized to cytosol and microsomes, similar to Sec14p; may be PITP regulator rather than actual PITP 145157..146041 Saccharomyces cerevisiae 853296 NP_012391.1 CDS YJL144W NC_001142.7 146356 146670 D Yjl144wp; Cytoplasmic hydrophilin of unknown function, possibly involved in the dessication response; expression induced by osmotic stress, starvation and during stationary phase; GFP-fusion protein is induced by the DNA-damaging agent MMS 146356..146670 Saccharomyces cerevisiae 853297 NP_012392.1 CDS TIM17 NC_001142.7 147098 147574 D Tim17p; Essential constituent of the mitochondrial inner membrane presequence translocase; interacts with Pam18p to recruit the presequence translocase-associated motor (PAM complex) and also required for protein sorting during import 147098..147574 Saccharomyces cerevisiae 853298 NP_012394.1 CDS YAK1 NC_001142.7 147964 150387 R Yak1p; Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal complement(147964..150387) Saccharomyces cerevisiae 853300 NP_012395.1 CDS RPB4 NC_001142.7 150958 151623 D Rpb4p; RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; dispensable under some environmental conditions; involved in export of mRNA to cytoplasm under stress conditions; involved in telomere maintenance 150958..151623 Saccharomyces cerevisiae 853301 NP_012396.1 CDS YUR1 NC_001142.7 151710 152996 R Yur1p; Mannosyltransferase of the KTR1 family, involved in protein N-glycosylation; located in the Golgi apparatus complement(151710..152996) Saccharomyces cerevisiae 853302 NP_012397.1 CDS TIF2 NC_001142.7 153501 154688 R Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; Tif2p complement(153501..154688) Saccharomyces cerevisiae 853303 NP_012398.1 CDS GLG2 NC_001142.7 154982 156124 R Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; Glg2p complement(154982..156124) Saccharomyces cerevisiae 853304 NP_878101.1 CDS YJL136W-A NC_001142.7 156166 156249 D Yjl136w-ap; Identified by SAGE 156166..156249 Saccharomyces cerevisiae 1466464 NP_012399.1 CDS RPS21B NC_001142.7 156547 157270 R Rps21bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Ap and has similarity to rat S21 ribosomal protein complement(join(156547..156786,157247..157270)) Saccharomyces cerevisiae 853305 NP_012401.1 CDS LCB3 NC_001142.7 158185 159414 D Long-chain base-1-phosphate phosphatase with specificity for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; Lcb3p 158185..159414 Saccharomyces cerevisiae 853307 NP_878102.1 CDS YJL133C-A NC_001142.7 159620 159844 R Yjl133c-ap; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(159620..159844) Saccharomyces cerevisiae 1466465 NP_012402.1 CDS MRS3 NC_001142.7 160616 161560 D Mrs3p; Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs4p; functions under low-iron conditions; may transport other cations in addition to iron 160616..161560 Saccharomyces cerevisiae 853308 NP_012403.1 CDS YJL132W NC_001142.7 161911 164163 D Yjl132wp; Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene 161911..164163 Saccharomyces cerevisiae 853309 NP_012404.1 CDS AIM23 NC_001142.7 164275 165345 R Putative protein of unknown function; the authentic non-tagged protein is detected in highly purified mitochondria; null mutant is viable, displays increased frequency of mitochondrial genome loss (petite formation); Aim23p complement(164275..165345) Saccharomyces cerevisiae 853310 NP_012405.1 CDS URA2 NC_001142.7 165720 172364 R Ura2p; Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP complement(165720..172364) Saccharomyces cerevisiae 853311 NP_012406.1 CDS TRK1 NC_001142.7 173596 177303 R Trk1p; Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation complement(173596..177303) Saccharomyces cerevisiae 853312 NP_012407.1 CDS PBS2 NC_001142.7 178094 180100 R Pbs2p; MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress complement(178094..180100) Saccharomyces cerevisiae 853313 NP_878103.1 CDS YJL127C-B NC_001142.7 181549 181707 R Yjl127c-bp; Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii complement(181549..181707) Saccharomyces cerevisiae 1466466 NP_012408.1 CDS SPT10 NC_001142.7 182296 184218 R Spt10p; Putative histone acetylase, sequence-specific activator of histone genes, binds specifically and highly cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box complement(182296..184218) Saccharomyces cerevisiae 853315 NP_012409.1 CDS NIT2 NC_001142.7 184499 185422 D Nit2p; Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member 184499..185422 Saccharomyces cerevisiae 853316 NP_012410.1 CDS GCD14 NC_001142.7 185526 186677 R Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression; Gcd14p complement(185526..186677) Saccharomyces cerevisiae 853317 NP_012411.1 CDS LSM1 NC_001142.7 187125 187643 R Lsm1p; Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs complement(187125..187643) Saccharomyces cerevisiae 853318 NP_012412.1 CDS YJL123C NC_001142.7 188003 189439 R Yjl123cp; Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); YJL123C is a non-essential gene complement(188003..189439) Saccharomyces cerevisiae 853319 NP_012413.1 CDS ALB1 NC_001142.7 189715 190242 D Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p; Alb1p 189715..190242 Saccharomyces cerevisiae 853320 NP_012414.1 CDS RPE1 NC_001142.7 190373 191089 R Rpe1p; D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress complement(190373..191089) Saccharomyces cerevisiae 853322 NP_012417.1 CDS YJL118W NC_001142.7 191638 192297 D Yjl118wp; Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein 191638..192297 Saccharomyces cerevisiae 853323 NP_012418.1 CDS PHO86 NC_001142.7 192530 193465 D Pho86p; Endoplasmic reticulum (ER) resident protein required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles 192530..193465 Saccharomyces cerevisiae 853325 NP_012419.1 CDS NCA3 NC_001142.7 193859 194872 R Protein that functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p ) of the Fo-F1 ATP synthase; member of the SUN family; Nca3p complement(193859..194872) Saccharomyces cerevisiae 853326 NP_012420.1 CDS ASF1 NC_001142.7 196285 197124 D Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; Asf1p 196285..197124 Saccharomyces cerevisiae 853327 NP_012421.1 CDS YJL113W NC_001142.7 197913 203325 D Yjl113wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(197913..198998,199000..203325) Saccharomyces cerevisiae 853330 NP_012422.1 CDS YJL114W NC_001142.7 197913 199157 D Yjl114wp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 197913..199157 Saccharomyces cerevisiae 853329 NP_012423.1 CDS MDV1 NC_001142.7 205301 207445 D Peripheral protein of the cytosolic face of the mitochondrial outer membrane, required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; Mdv1p 205301..207445 Saccharomyces cerevisiae 853332 NP_012424.1 CDS CCT7 NC_001142.7 207873 209525 D Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct7p 207873..209525 Saccharomyces cerevisiae 853333 NP_012425.1 CDS GZF3 NC_001142.7 209918 211573 R Gzf3p; GATA zinc finger protein and Dal80p homolog that negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p complement(209918..211573) Saccharomyces cerevisiae 853334 NP_012426.1 CDS UTP10 NC_001142.7 211996 217305 R Utp10p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(211996..217305) Saccharomyces cerevisiae 853335 NP_012427.1 CDS PRM10 NC_001142.7 217701 218852 R Prm10p; Pheromone-regulated protein, predicted to have 5 transmembrane segments complement(217701..218852) Saccharomyces cerevisiae 853336 NP_012428.1 CDS YJL107C NC_001142.7 218849 220012 R Yjl107cp; Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi complement(218849..220012) Saccharomyces cerevisiae 853337 NP_012429.1 CDS IME2 NC_001142.7 221386 223323 D Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p; Ime2p 221386..223323 Saccharomyces cerevisiae 853338 NP_012430.1 CDS SET4 NC_001142.7 225051 226733 D Set4p; Protein of unknown function, contains a SET domain 225051..226733 Saccharomyces cerevisiae 853339 NP_012431.1 CDS PAM16 NC_001142.7 227323 227772 D Pam16p; Constituent of the mitochondrial import motor associated with the presequence translocase, along with Ssc1p, Tim44p, Mge1p, and Pam18p; forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains J-like domain 227323..227772 Saccharomyces cerevisiae 853340 NP_012432.1 CDS GSM1 NC_001142.7 229021 230877 R Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis; Gsm1p complement(229021..230877) Saccharomyces cerevisiae 853341 NP_012433.1 CDS MEF2 NC_001142.7 231297 233756 D Mitochondrial elongation factor involved in translational elongation; Mef2p 231297..233756 Saccharomyces cerevisiae 853342 NP_012434.1 CDS GSH1 NC_001142.7 234316 236352 R Gsh1p; Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury complement(234316..236352) Saccharomyces cerevisiae 853344 NP_012435.1 CDS LSB6 NC_001142.7 237259 239082 D Phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; Lsb6p 237259..239082 Saccharomyces cerevisiae 853345 NP_012436.1 CDS CHS6 NC_001142.7 239410 241650 D Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; Chs6p 239410..241650 Saccharomyces cerevisiae 853346 NP_012437.1 CDS SAP185 NC_001142.7 242078 245254 D Sap185p; Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p 242078..245254 Saccharomyces cerevisiae 853347 NP_012438.1 CDS PHS1 NC_001142.7 245587 246240 D Protein of unknown function; homolog of mammalian PTPLA; involved in sphingolipid biosynthesis, protein trafficking; required for cell viability; Phs1p 245587..246240 Saccharomyces cerevisiae 853348 NP_012439.2 CDS MRPL49 NC_001142.7 246486 246971 D Mrpl49p; Mitochondrial ribosomal protein of the large subunit 246486..246971 Saccharomyces cerevisiae 853349 NP_012440.1 CDS BCK1 NC_001142.7 247250 251686 D Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; Bck1p 247250..251686 Saccharomyces cerevisiae 853350 NP_012441.1 CDS KHA1 NC_001142.7 251816 254437 R Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies; Kha1p complement(251816..254437) Saccharomyces cerevisiae 853351 NP_012442.1 CDS TOK1 NC_001142.7 254732 256807 R Tok1p; Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin complement(254732..256807) Saccharomyces cerevisiae 853352 NP_012443.1 CDS SRS2 NC_001142.7 257418 260942 D Srs2p; DNA helicase and DNA-dependent ATPase involved in DNA repair, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; affects genome stability by suppressing unscheduled homologous recombination 257418..260942 Saccharomyces cerevisiae 853353 NP_012444.2 CDS GWT1 NC_001142.7 261075 262547 R Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors; Gwt1p complement(261075..262547) Saccharomyces cerevisiae 853354 NP_012445.1 CDS DPB11 NC_001142.7 262752 265046 R Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control; Dpb11p complement(262752..265046) Saccharomyces cerevisiae 853355 NP_012446.1 CDS SIP4 NC_001142.7 265921 268410 D Sip4p; C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus 265921..268410 Saccharomyces cerevisiae 853356 NP_012447.1 CDS ARG3 NC_001142.7 268794 269810 D Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine; Arg3p 268794..269810 Saccharomyces cerevisiae 853357 NP_012448.1 CDS TRL1 NC_001142.7 269997 272480 R tRNA ligase, required for tRNA splicing; composed of three essential domains containing the phosphodiesterase, polynucleotide kinase, and ligase activities required for ligation; localized at the inner membrane of the nuclear envelope; Trl1p complement(269997..272480) Saccharomyces cerevisiae 853358 NP_012450.1 CDS EXO70 NC_001142.7 272822 274693 D Exo70p; Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis 272822..274693 Saccharomyces cerevisiae 853359 NP_012451.1 CDS ALY2 NC_001142.7 274857 277997 R Cytoplasmic protein of unknown function that interacts with the cyclin Pcl7p; phosphorylated in vitro by the cyclin-CDK complex, Pcl7p-Pho85p; identified as a potential Cdc28p substrate; mRNA is cell cycle regulated, peaking in M phase; Aly2p complement(274857..277997) Saccharomyces cerevisiae 853361 NP_012452.1 CDS TAX4 NC_001142.7 278836 280650 D Tax4p; Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p 278836..280650 Saccharomyces cerevisiae 853362 NP_012453.1 CDS IML2 NC_001142.7 281180 283375 D Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Iml2p 281180..283375 Saccharomyces cerevisiae 853363 NP_012454.1 CDS ARP4 NC_001142.7 283797 285266 R Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; Arp4p complement(283797..285266) Saccharomyces cerevisiae 853364 NP_012455.1 CDS SCP160 NC_001142.7 285553 289221 R Scp160p; Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins complement(285553..289221) Saccharomyces cerevisiae 853365 NP_012456.1 CDS PRY1 NC_001142.7 289870 290769 R Pry1p; Protein of unknown function, has similarity to Pry2p and Pry3p and to the plant PR-1 class of pathogen related proteins complement(289870..290769) Saccharomyces cerevisiae 853366 NP_012457.1 CDS PRY3 NC_001142.7 291331 293976 R Pry3p; Protein of unknown function, has similarity to Pry1p and Pry2p and to the plant PR-1 class of pathogen related proteins complement(291331..293976) Saccharomyces cerevisiae 853367 NP_878104.1 CDS YJL077W-B NC_001142.7 294042 294140 D Yjl077w-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 294042..294140 Saccharomyces cerevisiae 1466467 NP_012458.1 CDS ICS3 NC_001142.7 294661 295056 R Ics3p complement(294661..295056) Saccharomyces cerevisiae 853368 NP_012459.1 CDS NET1 NC_001142.7 295240 298809 D Net1p; Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure 295240..298809 Saccharomyces cerevisiae 853369 NP_012461.1 CDS SMC3 NC_001142.7 299152 302844 R Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member; Smc3p complement(299152..302844) Saccharomyces cerevisiae 853371 NP_012462.1 CDS JEM1 NC_001142.7 303176 305113 D Jem1p; DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 303176..305113 Saccharomyces cerevisiae 853372 NP_012463.1 CDS PSF2 NC_001142.7 305216 305857 R Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery; Psf2p complement(305216..305857) Saccharomyces cerevisiae 853373 NP_012464.1 CDS ARG2 NC_001142.7 306127 307851 D Arg2p; Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p 306127..307851 Saccharomyces cerevisiae 853374 NP_012465.1 CDS YJL070C NC_001142.7 307966 310632 R Yjl070cp; Putative protein of unknown function with similarity to AMP deaminases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YJL070C is a non-essential gene complement(307966..310632) Saccharomyces cerevisiae 853375 NP_012466.1 CDS UTP18 NC_001142.7 310917 312701 R Utp18p; Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data complement(310917..312701) Saccharomyces cerevisiae 853376 NP_012467.1 CDS YJL068C NC_001142.7 313011 313910 R Yjl068cp; Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D complement(313011..313910) Saccharomyces cerevisiae 853377 NP_012469.1 CDS MPM1 NC_001142.7 314109 314867 R Mpm1p; Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches complement(314109..314867) Saccharomyces cerevisiae 853379 NP_012470.1 CDS DLS1 NC_001142.7 315049 315552 R Subunit of ISW2/yCHRAC chromatin accessibility complex along with Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; Dls1p complement(315049..315552) Saccharomyces cerevisiae 853381 NP_012472.1 CDS MRPL8 NC_001142.7 315754 316470 R Mrpl8p; Mitochondrial ribosomal protein of the large subunit complement(315754..316470) Saccharomyces cerevisiae 853382 NP_076894.1 CDS YJL062W-A NC_001142.7 316718 316975 D Yjl062w-ap; Putative protein of unknown function, identified based on comparison to related yeast species; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 316718..316975 Saccharomyces cerevisiae 853383 NP_012473.1 CDS LAS21 NC_001142.7 317279 319771 D Las21p; Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity 317279..319771 Saccharomyces cerevisiae 853384 NP_012474.1 CDS NUP82 NC_001142.7 320011 322152 D Nup82p; Nucleoporin, subunit of the nuclear pore complex (NPC); forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC 320011..322152 Saccharomyces cerevisiae 853385 NP_012475.1 CDS BNA3 NC_001142.7 323381 324715 D Bna3p; Arylformamidase, involved in biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; potential Cdc28p substrate 323381..324715 Saccharomyces cerevisiae 853386 NP_012476.1 CDS YHC3 NC_001142.7 324959 326185 D Yhc3p; Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) 324959..326185 Saccharomyces cerevisiae 853387 NP_012477.1 CDS BIT61 NC_001142.7 326237 327868 R Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; Bit61p complement(326237..327868) Saccharomyces cerevisiae 853388 NP_012478.1 CDS IKS1 NC_001142.7 328113 330116 R Putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p; Iks1p complement(328113..330116) Saccharomyces cerevisiae 853389 NP_012479.1 CDS ZAP1 NC_001142.7 330426 333068 R Zinc-regulated transcription factor, binds to zinc-responsive promoter elements to induce transcription of certain genes in the presence of zinc; regulates its own transcription; contains seven zinc-finger domains; Zap1p complement(330426..333068) Saccharomyces cerevisiae 853390 NP_012480.1 CDS YJL055W NC_001142.7 333352 334089 D Yjl055wp; Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU 333352..334089 Saccharomyces cerevisiae 853391 NP_012481.1 CDS TIM54 NC_001142.7 334260 335696 D Tim54p; Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane 334260..335696 Saccharomyces cerevisiae 853392 NP_012482.1 CDS PEP8 NC_001142.7 335893 337032 D Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; Pep8p 335893..337032 Saccharomyces cerevisiae 853393 NP_076895.1 CDS YJL052C-A NC_001142.7 337879 337998 R Yjl052c-ap; Putative protein of unknown function, identified based on comparison to related yeast species complement(337879..337998) Saccharomyces cerevisiae 853394 NP_012483.1 CDS TDH1 NC_001142.7 338266 339264 D Tdh1p; Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall 338266..339264 Saccharomyces cerevisiae 853395 NP_012484.1 CDS IRC8 NC_001142.7 339782 342250 D Irc8p; Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52 foci 339782..342250 Saccharomyces cerevisiae 853396 NP_012485.1 CDS MTR4 NC_001142.7 342517 345738 D Mtr4p; Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA 342517..345738 Saccharomyces cerevisiae 853397 NP_012486.1 CDS YJL049W NC_001142.7 345968 347320 D Yjl049wp; Putative protein of unknown function; YJL049W is a non-essential gene 345968..347320 Saccharomyces cerevisiae 853398 NP_012487.1 CDS UBX6 NC_001142.7 347442 348632 R Ubx6p; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline complement(347442..348632) Saccharomyces cerevisiae 853399 NP_878105.1 CDS YJL047C-A NC_001142.7 349039 349173 R Yjl047c-ap; Putative protein of unknown function complement(349039..349173) Saccharomyces cerevisiae 1466468 NP_012488.1 CDS RTT101 NC_001142.7 349575 352103 R Rtt101p; Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; deletion phenotype suggests a role in anaphase progression; interacts with Mms22p and implicated in Mms22-dependent DNA repair; modified by the ubiquitin-like protein, Rub1p complement(349575..352103) Saccharomyces cerevisiae 853400 NP_012489.2 CDS AIM22 NC_001142.7 352381 353610 D Putative lipoate-protein ligase A family member; null mutant displays respiratory growth defect, decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim22p 352381..353610 Saccharomyces cerevisiae 853401 NP_012490.1 CDS YJL045W NC_001142.7 356019 357923 D Yjl045wp; Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner 356019..357923 Saccharomyces cerevisiae 853405 NP_012491.1 CDS GYP6 NC_001142.7 358295 359671 R Gyp6p; GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport complement(358295..359671) Saccharomyces cerevisiae 853406 NP_012492.1 CDS YJL043W NC_001142.7 360125 360898 D Yjl043wp; Putative protein of unknown function; YJL043W is a non-essential gene 360125..360898 Saccharomyces cerevisiae 853407 NP_012493.1 CDS MHP1 NC_001142.7 361244 365440 D Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins; Mhp1p 361244..365440 Saccharomyces cerevisiae 853408 NP_012494.1 CDS NSP1 NC_001142.7 365779 368368 D Nsp1p; Essential component of the nuclear pore complex, which mediates nuclear import and export join(365779,365898..368368) Saccharomyces cerevisiae 853409 NP_012495.1 CDS NUP192 NC_001142.7 368743 373794 R Nup192p; Essential structural subunit of the nuclear pore complex (NPC), localizes to the nuclear periphery of nuclear pores, homologous to human p205 complement(368743..373794) Saccharomyces cerevisiae 853410 NP_012496.1 CDS YJL038C NC_001142.7 375110 375769 R Yjl038cp; Putative protein of unknown function; expression induced during sporulation and repressed during vegetative growth by Sum1p and Hst1p; similar to adjacent open reading frame, YJL037W complement(375110..375769) Saccharomyces cerevisiae 853413 NP_012497.1 CDS IRC18 NC_001142.7 376657 377331 D Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52 foci; Irc18p 376657..377331 Saccharomyces cerevisiae 853414 NP_012498.1 CDS SNX4 NC_001142.7 378820 380091 D Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX domain; forms complex with Snx41p and Atg20p; Snx4p 378820..380091 Saccharomyces cerevisiae 853416 NP_012499.1 CDS TAD2 NC_001142.7 380244 380996 R Tad2p; Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs complement(380244..380996) Saccharomyces cerevisiae 853417 NP_012500.1 CDS KAR2 NC_001142.7 381322 383370 D Kar2p; ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p 381322..383370 Saccharomyces cerevisiae 853418 NP_012501.1 CDS HCA4 NC_001142.7 383832 386144 D Putative nucleolar DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis; Hca4p 383832..386144 Saccharomyces cerevisiae 853419 NP_012503.2 CDS BET4 NC_001142.7 386363 387433 R Bet4p; Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p complement(join(386363..387343,387431..387433)) Saccharomyces cerevisiae 853421 NP_012504.1 CDS MAD2 NC_001142.7 387652 388242 D Mad2p; Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p 387652..388242 Saccharomyces cerevisiae 853422 NP_012505.1 CDS VPS53 NC_001142.7 388380 390848 R Vps53p; Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting complement(388380..390848) Saccharomyces cerevisiae 853423 NP_012506.1 CDS YJL028W NC_001142.7 391306 391641 D Yjl028wp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments 391306..391641 Saccharomyces cerevisiae 853425 NP_012507.1 CDS YJL027C NC_001142.7 391828 392244 R Yjl027cp; Putative protein of unknown function complement(391828..392244) Saccharomyces cerevisiae 853426 NP_012508.1 CDS RNR2 NC_001142.7 392399 393598 D Rnr2p; Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits 392399..393598 Saccharomyces cerevisiae 853427 NP_012509.1 CDS RRN7 NC_001142.7 393962 395506 D Rrn7p; Protein involved in the transcription of 35S rRNA genes by RNA polymerase I; component of the core factor (CF) complex also composed of Rrn11p, Rrn6p and TATA-binding protein 393962..395506 Saccharomyces cerevisiae 853428 NP_012510.1 CDS APS3 NC_001142.7 395926 396587 R Small subunit of the clathrin-associated adaptor complex AP-3, which is involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; Aps3p complement(join(395926..396488,396566..396587)) Saccharomyces cerevisiae 853429 NP_012511.1 CDS PET130 NC_001142.7 397350 398393 R Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Pet130p complement(397350..398393) Saccharomyces cerevisiae 853432 NP_012514.2 CDS BBC1 NC_001142.7 398932 402405 R Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches; Bbc1p complement(398932..402405) Saccharomyces cerevisiae 853433 NP_012515.2 CDS MPS3 NC_001142.7 402892 404940 D Mps3p; Essential integral membrane protein required for spindle pole body duplication and for nuclear fusion, localizes to the spindle pole body half bridge, interacts with DnaJ-like chaperone Jem1p and with centrin homolog Cdc31p 402892..404940 Saccharomyces cerevisiae 853434 NP_012518.2 CDS YJL016W NC_001142.7 405583 407268 D Yjl016wp; Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species 405583..407268 Saccharomyces cerevisiae 853436 NP_012520.1 CDS CCT3 NC_001142.7 407552 409156 D Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct3p 407552..409156 Saccharomyces cerevisiae 853438 NP_012521.1 CDS MAD3 NC_001142.7 409487 411034 R Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; Mad3p complement(409487..411034) Saccharomyces cerevisiae 853439 NP_012522.2 CDS VTC4 NC_001142.7 411228 413393 R Vtc4p; Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion complement(411228..413393) Saccharomyces cerevisiae 853441 NP_012523.1 CDS RPC17 NC_001142.7 414279 414764 R Rpc17p; RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex complement(414279..414764) Saccharomyces cerevisiae 853442 NP_012524.1 CDS NOP9 NC_001142.7 417556 419556 R Nop9p; Essential component of pre-40S ribosomes that is required for early cleavages of 35S pre-rRNA and hence formation of 18S rRNA; binds RNA in vitro and contains multiple pumilio-like repeats complement(417556..419556) Saccharomyces cerevisiae 853445 NP_012526.1 CDS CCT8 NC_001142.7 419951 421657 R Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct8p complement(419951..421657) Saccharomyces cerevisiae 853447 NP_012528.1 CDS CTK2 NC_001142.7 423132 424103 R Ctk2p; Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins complement(423132..424103) Saccharomyces cerevisiae 853450 NP_012529.1 CDS CYR1 NC_001142.7 425151 431231 D Cyr1p; Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation 425151..431231 Saccharomyces cerevisiae 853452 NP_012530.1 CDS SYS1 NC_001142.7 431583 432194 R Sys1p; Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation complement(431583..432194) Saccharomyces cerevisiae 853453 NP_012531.1 CDS COX16 NC_001142.7 432638 432994 D Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase; Cox16p 432638..432994 Saccharomyces cerevisiae 853454 NP_012532.1 CDS OST1 NC_001142.7 433215 434645 R Ost1p; Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins complement(433215..434645) Saccharomyces cerevisiae 853455 NP_012533.1 CDS PRE3 NC_001142.7 435157 435920 D Pre3p; 20S proteasome beta-type subunit, responsible for cleavage after acidic residues in peptides join(435157..435221,435338..435920) Saccharomyces cerevisiae 853456 NP_012534.1 CDS AVT1 NC_001142.7 436796 438604 D Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt1p 436796..438604 Saccharomyces cerevisiae 853457 NP_012535.1 CDS MPP10 NC_001142.7 438858 440639 D Mpp10p; Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p 438858..440639 Saccharomyces cerevisiae 853458 NP_012536.2 CDS YJR003C NC_001142.7 440987 442546 R Yjr003cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(440987..442546) Saccharomyces cerevisiae 853459 NP_012537.1 CDS SAG1 NC_001142.7 442902 444854 R Sag1p; Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor complement(442902..444854) Saccharomyces cerevisiae 853460 NP_012538.1 CDS APL1 NC_001142.7 445916 448018 D Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex; Apl1p 445916..448018 Saccharomyces cerevisiae 853461 NP_878106.1 CDS YJR005C-A NC_001142.7 448470 448751 R Yjr005c-ap; Putative protein of unknown function, originally identified as a syntenic homolog of an Ashbya gossypii gene complement(448470..448751) Saccharomyces cerevisiae 1466469 NP_012539.1 CDS POL31 NC_001142.7 449195 450658 D Pol31p; DNA polymerase III (delta) subunit, essential for cell viability; involved in DNA replication and DNA repair 449195..450658 Saccharomyces cerevisiae 853462 NP_012540.1 CDS SUI2 NC_001142.7 451013 451927 D Sui2p; Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP 451013..451927 Saccharomyces cerevisiae 853463 NP_012541.1 CDS YJR008W NC_001142.7 452423 453439 D Yjr008wp; Putative protein of unknown function; expression repressed by inosine and choline in an Opi1p-dependent manner; expression induced by mild heat-stress on a non-fermentable carbon source. 452423..453439 Saccharomyces cerevisiae 853464 NP_012542.1 CDS TDH2 NC_001142.7 453676 454674 R Tdh2p; Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall complement(453676..454674) Saccharomyces cerevisiae 853465 NP_012543.1 CDS MET3 NC_001142.7 456232 457767 D Met3p; ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism 456232..457767 Saccharomyces cerevisiae 853466 NP_012544.1 CDS SPC1 NC_001142.7 458070 458354 R Subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER), homolog of the SPC12 subunit of mammalian signal peptidase complex; Spc1p complement(458070..458354) Saccharomyces cerevisiae 853467 NP_012545.1 CDS YJR011C NC_001142.7 458634 459419 R Yjr011cp; Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS complement(458634..459419) Saccharomyces cerevisiae 853468 NP_012546.1 CDS YJR012C NC_001142.7 459788 460411 R Yjr012cp; Putative protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W complement(459788..460411) Saccharomyces cerevisiae 853469 NP_012547.2 CDS GPI14 NC_001142.7 460375 461586 D Gpi14p; Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M 460375..461586 Saccharomyces cerevisiae 853470 NP_012548.1 CDS TMA22 NC_001142.7 461822 462418 D Tma22p; Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1 461822..462418 Saccharomyces cerevisiae 853471 NP_012549.1 CDS YJR015W NC_001142.7 462714 464246 D Yjr015wp; Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene 462714..464246 Saccharomyces cerevisiae 853472 NP_012550.1 CDS ILV3 NC_001142.7 464444 466201 R Ilv3p; Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids complement(464444..466201) Saccharomyces cerevisiae 853473 NP_012551.2 CDS ESS1 NC_001142.7 466514 467026 R Peptidylprolyl-cis/trans-isomerase (PPIase) specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNA polymerase II large subunit (Rpo21p) C-terminal domain; Ess1p complement(466514..467026) Saccharomyces cerevisiae 853475 NP_012553.1 CDS TES1 NC_001142.7 467225 468274 R Tes1p; Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids complement(467225..468274) Saccharomyces cerevisiae 853477 NP_012555.1 CDS REC107 NC_001142.7 468548 469572 R Rec107p; Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation complement(join(468548..469176,469257..469572)) Saccharomyces cerevisiae 853478 NP_012556.2 CDS LSM8 NC_001142.7 469777 470106 D Lsm8p; Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA 469777..470106 Saccharomyces cerevisiae 853479 NP_012558.1 CDS MDE1 NC_001142.7 470223 470957 R Putative methylthio-ribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant; Mde1p complement(470223..470957) Saccharomyces cerevisiae 853481 NP_012559.1 CDS BNA1 NC_001142.7 471131 471664 R Bna1p; 3-hydroxyanthranilic acid dioxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway complement(471131..471664) Saccharomyces cerevisiae 853482 NP_012560.1 CDS YJR027W NC_001142.7 472753 478021 D Yjr027wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(472753..474054,474056..478021) Saccharomyces cerevisiae 853484 NP_012561.1 CDS YJR026W NC_001142.7 472753 474075 D Yjr026wp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 472753..474075 Saccharomyces cerevisiae 853483 NP_012562.1 CDS YJR029W NC_001142.7 478337 483605 D Yjr029wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(478337..479637,479639..483605) Saccharomyces cerevisiae 853486 NP_012563.1 CDS YJR028W NC_001142.7 478337 479659 D Yjr028wp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 478337..479659 Saccharomyces cerevisiae 853485 NP_012564.1 CDS YJR030C NC_001142.7 483952 486189 R Yjr030cp; Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; YJR030C is a non-essential gene complement(483952..486189) Saccharomyces cerevisiae 853487 NP_012565.1 CDS GEA1 NC_001142.7 486579 490805 R Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea2p; Gea1p complement(486579..490805) Saccharomyces cerevisiae 853488 NP_012566.1 CDS CPR7 NC_001142.7 491074 492255 D Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; Cpr7p 491074..492255 Saccharomyces cerevisiae 853489 NP_012567.1 CDS RAV1 NC_001142.7 492371 496444 R Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate; Rav1p complement(492371..496444) Saccharomyces cerevisiae 853490 NP_012568.1 CDS PET191 NC_001142.7 496676 497002 D Protein required for assembly of cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Pet191p 496676..497002 Saccharomyces cerevisiae 853491 NP_012569.1 CDS RAD26 NC_001142.7 497348 500605 D Rad26p; Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSB protein 497348..500605 Saccharomyces cerevisiae 853492 NP_012570.1 CDS HUL4 NC_001142.7 500706 503384 R Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability; Hul4p complement(500706..503384) Saccharomyces cerevisiae 853494 NP_012573.1 CDS YJR039W NC_001142.7 503929 507294 D Yjr039wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 503929..507294 Saccharomyces cerevisiae 853495 NP_012574.1 CDS GEF1 NC_001142.7 507739 510078 D Gef1p; Chloride channel localized to late- or post-Golgi vesicles, involved in iron metabolism; highly homologous to voltage-gated chloride channels in vertebrates 507739..510078 Saccharomyces cerevisiae 853497 NP_012575.1 CDS URB2 NC_001142.7 510232 513756 R Urb2p; Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis complement(510232..513756) Saccharomyces cerevisiae 853498 NP_012576.1 CDS NUP85 NC_001142.7 514048 516282 D Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking; Nup85p 514048..516282 Saccharomyces cerevisiae 853499 NP_012577.1 CDS POL32 NC_001142.7 516454 517506 R Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p; Pol32p complement(516454..517506) Saccharomyces cerevisiae 853500 NP_012578.1 CDS VPS55 NC_001142.7 518756 519178 R Vps55p; Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) complement(518756..519178) Saccharomyces cerevisiae 853502 NP_012579.1 CDS SSC1 NC_001142.7 519631 521595 R Ssc1p; Mitochondrial matrix ATPase, subunit of the presequence translocase-associated protein import motor (PAM) and of SceI endonuclease; involved in protein folding and translocation into the matrix; phosphorylated; member of HSP70 family complement(519631..521595) Saccharomyces cerevisiae 853503 NP_012580.1 CDS TAH11 NC_001142.7 522041 523855 D Tah11p; DNA replication licensing factor, required for pre-replication complex assembly 522041..523855 Saccharomyces cerevisiae 853504 NP_012581.1 CDS ANB1 NC_001142.7 524901 525374 R Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions; Anb1p complement(524901..525374) Saccharomyces cerevisiae 853506 NP_012582.1 CDS CYC1 NC_001142.7 526328 526657 D Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; Cyc1p 526328..526657 Saccharomyces cerevisiae 853507 NP_012583.1 CDS UTR1 NC_001142.7 526877 528469 R Utr1p; ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p) complement(526877..528469) Saccharomyces cerevisiae 853508 NP_012584.1 CDS ISY1 NC_001142.7 528690 529397 D Isy1p; Component of the spliceosome complex involved in pre-mRNA splicing, auxiliary splicing factor that may modulate Syf1p activity and help optimize splicing; isy1 syf2 double mutation activates the spindle checkpoint, causing cell cycle arrest 528690..529397 Saccharomyces cerevisiae 853509 NP_012585.1 CDS OSM1 NC_001142.7 529854 531359 D Osm1p; Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity 529854..531359 Saccharomyces cerevisiae 853510 NP_012586.1 CDS RAD7 NC_001142.7 532055 533752 D Rad7p; Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex 532055..533752 Saccharomyces cerevisiae 853512 NP_012587.1 CDS BFA1 NC_001142.7 534020 535744 D Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis; Bfa1p 534020..535744 Saccharomyces cerevisiae 853513 NP_012588.1 CDS YJR054W NC_001142.7 536049 537542 D Yjr054wp; Vacuolar protein of unknown function; potential Cdc28p substrate 536049..537542 Saccharomyces cerevisiae 853514 NP_012589.1 CDS HIT1 NC_001142.7 538765 539259 D Protein of unknown function, required for growth at high temperature; Hit1p 538765..539259 Saccharomyces cerevisiae 853516 NP_012590.1 CDS YJR056C NC_001142.7 541785 542495 R Yjr056cp complement(541785..542495) Saccharomyces cerevisiae 853518 NP_012591.1 CDS CDC8 NC_001142.7 544055 544705 D Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p; Cdc8p 544055..544705 Saccharomyces cerevisiae 853520 NP_012592.1 CDS APS2 NC_001142.7 544725 545168 R Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex; Aps2p complement(544725..545168) Saccharomyces cerevisiae 853521 NP_012593.1 CDS PTK2 NC_001142.7 545780 548236 D Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake; Ptk2p 545780..548236 Saccharomyces cerevisiae 853522 NP_012594.1 CDS CBF1 NC_001142.7 548752 549807 D Helix-loop-helix protein that binds the motif CACRTG, which is present at several sites including MET gene promoters and centromere DNA element I (CDEI); required for nucleosome positioning at this motif; targets Isw1p to DNA; Cbf1p 548752..549807 Saccharomyces cerevisiae 853523 NP_012595.1 CDS YJR061W NC_001142.7 550504 553311 D Yjr061wp; Putative protein of unknown function; non-essential gene with similarity to Mnn4, a putative membrane protein involved in glycosylation; transcription repressed by Rm101p 550504..553311 Saccharomyces cerevisiae 853524 NP_012596.1 CDS NTA1 NC_001142.7 553469 554842 R Nta1p; Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation complement(553469..554842) Saccharomyces cerevisiae 853525 NP_012597.1 CDS RPA12 NC_001142.7 555188 555565 D Rpa12p; RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex 555188..555565 Saccharomyces cerevisiae 853526 NP_012598.1 CDS CCT5 NC_001142.7 555907 557595 D Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; Cct5p 555907..557595 Saccharomyces cerevisiae 853527 NP_012599.1 CDS ARP3 NC_001142.7 557802 559151 R Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; Arp3p complement(557802..559151) Saccharomyces cerevisiae 853528 NP_012600.1 CDS TOR1 NC_001142.7 559409 566821 D Tor1p; PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis 559409..566821 Saccharomyces cerevisiae 853529 NP_012601.1 CDS YAE1 NC_001142.7 567012 567437 R Yae1p; Essential protein of unknown function complement(567012..567437) Saccharomyces cerevisiae 853530 NP_012602.1 CDS RFC2 NC_001142.7 567636 568697 D Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc2p 567636..568697 Saccharomyces cerevisiae 853531 NP_012603.1 CDS HAM1 NC_001142.7 568799 569392 R Ham1p; Conserved protein with deoxyribonucleoside triphosphate pyrophosphohydrolase activity, mediates exclusion of noncanonical purines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine complement(568799..569392) Saccharomyces cerevisiae 853532 NP_012604.1 CDS LIA1 NC_001142.7 569614 570591 R Lia1p; Deoxyhypusine hydroxylase, a HEAT-repeat containing metalloenzyme that catalyses hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning complement(569614..570591) Saccharomyces cerevisiae 853534 NP_012606.1 CDS NPA3 NC_001142.7 570960 572117 R Npa3p; Essential, conserved, cytoplasmic ATPase; phosphorylated by the Pcl1p-Pho85p kinase complex complement(570960..572117) Saccharomyces cerevisiae 853535 NP_012607.1 CDS OPI3 NC_001142.7 572308 572928 R Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis; Opi3p complement(572308..572928) Saccharomyces cerevisiae 853536 NP_012608.1 CDS MOG1 NC_001142.7 573088 573744 D Mog1p; Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p 573088..573744 Saccharomyces cerevisiae 853537 NP_012609.1 CDS HOC1 NC_001142.7 573974 575164 D Hoc1p; Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele 573974..575164 Saccharomyces cerevisiae 853538 NP_012610.1 CDS CDC11 NC_001142.7 575347 576594 R Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM; Cdc11p complement(575347..576594) Saccharomyces cerevisiae 853539 NP_012611.1 CDS MIR1 NC_001142.7 577248 578183 R Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated; Mir1p complement(577248..578183) Saccharomyces cerevisiae 853540 NP_012612.1 CDS BNA2 NC_001142.7 578853 580214 D Tryptophan 2,3-dioxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; Bna2p 578853..580214 Saccharomyces cerevisiae 853541 NP_012613.1 CDS YJR079W NC_001142.7 580198 581232 D Yjr079wp; Putative protein of unknown function; mutation results in impaired mitochondrial respiration join(580198..580340,581046..581232) Saccharomyces cerevisiae 853542 NP_012614.1 CDS AIM24 NC_001142.7 580425 581609 R Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss and reduced growth rate in minimal glycerol media; Aim24p complement(580425..581609) Saccharomyces cerevisiae 853543 NP_012615.1 CDS EAF6 NC_001142.7 581907 582248 R Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3; Eaf6p complement(581907..582248) Saccharomyces cerevisiae 853544 NP_012616.1 CDS ACF4 NC_001142.7 582601 583530 R Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate; Acf4p complement(582601..583530) Saccharomyces cerevisiae 853545 NP_012617.1 CDS CSN12 NC_001142.7 583726 584997 D Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; Csn12p 583726..584997 Saccharomyces cerevisiae 853546 NP_012618.1 CDS YJR085C NC_001142.7 585113 585430 R Yjr085cp; Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(585113..585430) Saccharomyces cerevisiae 853547 NP_012619.1 CDS STE18 NC_001142.7 586061 586393 D Ste18p; G protein gamma subunit, forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling 586061..586393 Saccharomyces cerevisiae 853548 NP_012621.1 CDS YJR088C NC_001142.7 586488 587366 R Yjr088cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum complement(586488..587366) Saccharomyces cerevisiae 853550 NP_012622.1 CDS BIR1 NC_001142.7 587711 590575 D Essential chromosomal passenger protein involved in coordinating cell cycle events for proper chromosome segregation; C-terminal region binds Sli15p, and the middle region, upon phosphorylation, localizes Cbf2p to the spindle at anaphase; Bir1p 587711..590575 Saccharomyces cerevisiae 853551 NP_012623.1 CDS GRR1 NC_001142.7 590865 594320 R Grr1p; F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification complement(590865..594320) Saccharomyces cerevisiae 853552 NP_012624.1 CDS JSN1 NC_001142.7 595054 598329 R Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells; Jsn1p complement(595054..598329) Saccharomyces cerevisiae 853553 NP_012625.2 CDS BUD4 NC_001142.7 598728 603074 D Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate; Bud4p 598728..603074 Saccharomyces cerevisiae 853554 NP_012626.1 CDS FIP1 NC_001142.7 603216 604199 R Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; Fip1p complement(603216..604199) Saccharomyces cerevisiae 853555 NP_012627.1 CDS IME1 NC_001142.7 604565 605647 R Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p; Ime1p complement(604565..605647) Saccharomyces cerevisiae 853556 NP_012628.1 CDS RPL43B NC_001142.7 608301 608854 D Rpl43bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl43Ap and has similarity to rat L37a ribosomal protein join(608301..608302,608578..608854) Saccharomyces cerevisiae 853557 NP_012629.1 CDS SFC1 NC_001142.7 609769 610737 D Sfc1p; Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization 609769..610737 Saccharomyces cerevisiae 853558 NP_012630.1 CDS YJR096W NC_001142.7 611191 612039 D Yjr096wp; Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS 611191..612039 Saccharomyces cerevisiae 853559 NP_012631.1 CDS JJJ3 NC_001142.7 612409 612927 D Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein; Jjj3p 612409..612927 Saccharomyces cerevisiae 853560 NP_012632.1 CDS YJR098C NC_001142.7 613182 615149 R Yjr098cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(613182..615149) Saccharomyces cerevisiae 853561 NP_012633.1 CDS YUH1 NC_001142.7 615569 616279 D Yuh1p; Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p 615569..616279 Saccharomyces cerevisiae 853562 NP_012634.1 CDS AIM25 NC_001142.7 616344 617327 R Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss (petite formation); similar to murine NOR1; Aim25p complement(616344..617327) Saccharomyces cerevisiae 853563 NP_012635.1 CDS RSM26 NC_001142.7 618227 619027 D Rsm26p; Mitochondrial ribosomal protein of the small subunit 618227..619027 Saccharomyces cerevisiae 853565 NP_012636.1 CDS VPS25 NC_001142.7 619150 619758 R Vps25p; Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome complement(619150..619758) Saccharomyces cerevisiae 853566 NP_012637.1 CDS URA8 NC_001142.7 620747 622441 D Ura8p; Minor CTP synthase isozyme (see also URA7), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis 620747..622441 Saccharomyces cerevisiae 853567 NP_012638.1 CDS SOD1 NC_001142.7 622542 623006 R Sod1p; Cytosolic superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans complement(622542..623006) Saccharomyces cerevisiae 853568 NP_012639.1 CDS ADO1 NC_001142.7 623573 624595 D Ado1p; Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle 623573..624595 Saccharomyces cerevisiae 853569 NP_012640.1 CDS ECM27 NC_001142.7 624830 627007 D Non-essential protein of unknown function; Ecm27p 624830..627007 Saccharomyces cerevisiae 853570 NP_012641.1 CDS YJR107W NC_001142.7 627333 628319 D Yjr107wp; Putative protein of unknown function; has sequence or structural similarity to lipases 627333..628319 Saccharomyces cerevisiae 853571 NP_012642.1 CDS ABM1 NC_001142.7 628706 629077 D Protein of unknown function, required for normal microtubule organization; Abm1p 628706..629077 Saccharomyces cerevisiae 853572 NP_012643.1 CDS CPA2 NC_001142.7 629579 632935 R Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; Cpa2p complement(629579..632935) Saccharomyces cerevisiae 853573 NP_012644.1 CDS YMR1 NC_001142.7 633609 635675 D Ymr1p; Phosphatidylinositol 3-phosphate [PI(3)P] phosphatase, regulates the localization and levels of PI(3)P; involved in cytoplasm to vacuole (CVT) transport; has similarity to the conserved myotubularin dual specificity phosphatase family 633609..635675 Saccharomyces cerevisiae 853574 NP_012645.1 CDS YJR111C NC_001142.7 635849 636700 R Yjr111cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria complement(635849..636700) Saccharomyces cerevisiae 853575 NP_012646.1 CDS NNF1 NC_001142.7 637024 637629 D Nnf1p; Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation 637024..637629 Saccharomyces cerevisiae 853576 NP_878107.1 CDS YJR112W-A NC_001142.7 637784 638162 D Yjr112w-ap; Putative protein of unknown function; identified based on homology to Ashbya gossypii join(637784..637803,637853..638162) Saccharomyces cerevisiae 1466470 NP_012647.1 CDS RSM7 NC_001142.7 638226 638969 R Rsm7p; Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein complement(638226..638969) Saccharomyces cerevisiae 853578 NP_012649.1 CDS YJR115W NC_001142.7 639936 640445 D Yjr115wp; Putative protein of unknown function 639936..640445 Saccharomyces cerevisiae 853579 NP_012650.1 CDS YJR116W NC_001142.7 640819 641658 D Yjr116wp; Putative protein of unknown function 640819..641658 Saccharomyces cerevisiae 853580 NP_012651.1 CDS STE24 NC_001142.7 642001 643362 D Ste24p; Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans 642001..643362 Saccharomyces cerevisiae 853581 NP_012652.1 CDS ILM1 NC_001142.7 643484 644095 R Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth; Ilm1p complement(643484..644095) Saccharomyces cerevisiae 853582 NP_012653.1 CDS JHD2 NC_001142.7 644298 646484 R JmjC domain family histone demethylase specific for H3-K4 (lysine at position 4 of the histone H3 protein); removes methyl groups specifically added by Set1p methyltransferase; Jhd2p complement(644298..646484) Saccharomyces cerevisiae 853583 NP_012654.1 CDS YJR120W NC_001142.7 647120 647470 D Yjr120wp; Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p 647120..647470 Saccharomyces cerevisiae 853584 NP_012655.1 CDS ATP2 NC_001142.7 647601 649136 D Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated; Atp2p 647601..649136 Saccharomyces cerevisiae 853585 NP_012656.1 CDS IBA57 NC_001142.7 649770 651263 D Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system; Iba57p 649770..651263 Saccharomyces cerevisiae 853586 NP_012657.1 CDS RPS5 NC_001142.7 651895 652572 D Rps5p; Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins 651895..652572 Saccharomyces cerevisiae 853587 NP_012658.1 CDS YJR124C NC_001142.7 652886 654232 R Yjr124cp; Putative protein of unknown function; expression induced under calcium shortage complement(652886..654232) Saccharomyces cerevisiae 853588 NP_012659.1 CDS ENT3 NC_001142.7 654731 655957 R Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p; Ent3p complement(654731..655957) Saccharomyces cerevisiae 853589 NP_012660.1 CDS VPS70 NC_001142.7 656248 658683 R Vps70p; Protein of unknown function involved in vacuolar protein sorting complement(656248..658683) Saccharomyces cerevisiae 853590 NP_012661.1 CDS RSF2 NC_001142.7 658911 663053 R Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; Rsf2p complement(658911..663053) Saccharomyces cerevisiae 853592 NP_012663.1 CDS YJR129C NC_001142.7 663994 665013 R Yjr129cp; Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(663994..665013) Saccharomyces cerevisiae 853593 NP_012664.1 CDS STR2 NC_001142.7 665212 667131 R Str2p; Cystathionine gamma-synthase, converts cysteine into cystathionine complement(665212..667131) Saccharomyces cerevisiae 853594 NP_012665.1 CDS MNS1 NC_001142.7 667638 669287 D Mns1p; Alpha-1,2-mannosidase involved in ER quality control; catalyzes the removal of one mannose residue from Man9GlcNAc to produce a single isomer of Man8GlcNAc in N-linked oligosaccharide biosynthesis; integral to ER membrane 667638..669287 Saccharomyces cerevisiae 853595 NP_012666.1 CDS NMD5 NC_001142.7 669516 672662 D Nmd5p; Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog 669516..672662 Saccharomyces cerevisiae 853596 NP_012667.1 CDS XPT1 NC_001142.7 672985 673614 D Xpt1p; Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine 672985..673614 Saccharomyces cerevisiae 853597 NP_012668.1 CDS SGM1 NC_001142.7 673723 675846 R Sgm1p; Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus complement(673723..675846) Saccharomyces cerevisiae 853598 NP_012669.1 CDS MCM22 NC_001142.7 676053 676772 R Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner; Mcm22p complement(676053..676772) Saccharomyces cerevisiae 853599 NP_058168.1 CDS TIM8 NC_001142.7 676965 677228 D Tim8p; Mitochondrial intermembrane space protein mediating import and insertion of polytopic inner membrane proteins; homolog of human DDP1 (deafness dystonia peptide 1) which is mutated in the X-linked Mohr-Tranebjaerg syndrome 676965..677228 Saccharomyces cerevisiae 853600 NP_012670.1 CDS YJR136C NC_001142.7 677435 678700 R Yjr136cp; Putative protein of unknown function; may interact with mitochondrial ribosomal protein Rsm23p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(677435..678700) Saccharomyces cerevisiae 853601 NP_116579.1 CDS ECM17 NC_001142.7 678951 683279 R Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine; Ecm17p complement(678951..683279) Saccharomyces cerevisiae 853602 NP_012672.1 CDS IML1 NC_001142.7 684561 689315 D Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; Iml1p 684561..689315 Saccharomyces cerevisiae 853603 NP_012673.1 CDS HOM6 NC_001142.7 689439 690518 R Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions; Hom6p complement(689439..690518) Saccharomyces cerevisiae 853604 NP_012674.1 CDS HIR3 NC_001142.7 690744 695690 R Transcriptional corepressor involved in the cell cycle-regulated transcription of histone genes HTA1, HTB1, HHT1, and HHT2; involved in position-dependent gene silencing and nucleosome reassembly; Hir3p complement(690744..695690) Saccharomyces cerevisiae 853605 NP_012675.1 CDS YJR141W NC_001142.7 695900 696943 D Yjr141wp; Putative protein of unknown function; may be involved in mRNA processing; YJR141W is an essential gene 695900..696943 Saccharomyces cerevisiae 853606 NP_012676.1 CDS YJR142W NC_001142.7 697135 698163 D Yjr142wp; Putative protein of unknown function; GST fusion protein is a Dbf2-Mob1 phosphoylation target in a proteome chip analysis; synthetic lethal with PH085 deletion 697135..698163 Saccharomyces cerevisiae 853607 NP_012677.1 CDS PMT4 NC_001142.7 698320 700608 R Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals; Pmt4p complement(698320..700608) Saccharomyces cerevisiae 853608 NP_012678.1 CDS MGM101 NC_001142.7 700876 701685 D Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage; Mgm101p 700876..701685 Saccharomyces cerevisiae 853609 NP_012679.1 CDS RPS4A NC_001142.7 702021 703062 R Rps4ap; Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein complement(join(702021..702792,703049..703062)) Saccharomyces cerevisiae 853610 NP_012681.1 CDS HMS2 NC_001142.7 704190 705266 D Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; Hms2p 704190..705266 Saccharomyces cerevisiae 853612 NP_012682.1 CDS BAT2 NC_001142.7 705738 706868 D Cytosolic branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; Bat2p 705738..706868 Saccharomyces cerevisiae 853613 NP_012683.1 CDS YJR149W NC_001142.7 707154 708368 D Yjr149wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 707154..708368 Saccharomyces cerevisiae 853614 NP_012684.1 CDS DAN1 NC_001142.7 708805 709701 R Dan1p; Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth complement(708805..709701) Saccharomyces cerevisiae 853615 NP_012685.1 CDS DAN4 NC_001142.7 712249 715734 R Dan4p; Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth complement(712249..715734) Saccharomyces cerevisiae 853616 NP_878108.1 CDS YJR151W-A NC_001142.7 717574 717624 D Yjr151w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 717574..717624 Saccharomyces cerevisiae 1466471 NP_012686.1 CDS DAL5 NC_001142.7 719660 721291 D Dal5p; Allantoin permease; ureidosuccinate permease; expression is constitutive but sensitive to nitrogen catabolite repression 719660..721291 Saccharomyces cerevisiae 853617 NP_012687.1 CDS PGU1 NC_001142.7 722809 723894 D Pgu1p; Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins 722809..723894 Saccharomyces cerevisiae 853618 NP_012688.1 CDS YJR154W NC_001142.7 725778 726818 D Yjr154wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 725778..726818 Saccharomyces cerevisiae 853619 NP_012689.1 CDS AAD10 NC_001142.7 727399 728265 D Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; Aad10p 727399..728265 Saccharomyces cerevisiae 853620 NP_012690.1 CDS THI11 NC_001142.7 728568 729590 R Thi11p; Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 complement(728568..729590) Saccharomyces cerevisiae 853621 NP_012692.1 CDS HXT16 NC_001142.7 732434 734137 D Protein of unknown function with similarity to hexose transporter family members, expression is repressed by high levels of glucose; Hxt16p 732434..734137 Saccharomyces cerevisiae 853623 NP_012693.1 CDS SOR1 NC_001142.7 736038 737111 D Sorbitol dehydrogenase; expression is induced in the presence of sorbitol; Sor1p 736038..737111 Saccharomyces cerevisiae 853624 NP_012694.1 CDS MPH3 NC_001142.7 738002 739810 R Mph3p; Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication complement(738002..739810) Saccharomyces cerevisiae 853625 NP_012695.1 CDS COS5 NC_001142.7 742842 743993 R Protein of unknown function, member the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos5p complement(742842..743993) Saccharomyces cerevisiae 853626 NP_012698.1 CDS PAU16 NC_001143.7 1811 2182 R Putative protein of unknown function; Pau16p complement(1811..2182) Saccharomyces cerevisiae 853656 NP_012700.1 CDS YKL222C NC_001143.7 3504 5621 R Ykl222cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine complement(3504..5621) Saccharomyces cerevisiae 853658 NP_012701.1 CDS MCH2 NC_001143.7 6108 7529 D Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport; Mch2p 6108..7529 Saccharomyces cerevisiae 853659 NP_012702.1 CDS FRE2 NC_001143.7 9092 11227 R Fre2p; Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels complement(9092..11227) Saccharomyces cerevisiae 853660 NP_012703.1 CDS COS9 NC_001143.7 14485 15708 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos9p 14485..15708 Saccharomyces cerevisiae 853661 NP_012704.1 CDS SRY1 NC_001143.7 17359 18339 R Sry1p; 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required for survival in the presence of hydroxyaspartate complement(17359..18339) Saccharomyces cerevisiae 853662 NP_012705.1 CDS JEN1 NC_001143.7 22234 24084 D Lactate transporter, required for uptake of lactate and pyruvate; phosphorylated; expression is derepressed by transcriptional activator Cat8p during respiratory growth, and repressed in the presence of glucose, fructose, and mannose; Jen1p 22234..24084 Saccharomyces cerevisiae 853663 NP_012706.1 CDS URA1 NC_001143.7 25216 26160 D Ura1p; Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid 25216..26160 Saccharomyces cerevisiae 853664 NP_012707.1 CDS YKL215C NC_001143.7 26828 30688 R Ykl215cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(26828..30688) Saccharomyces cerevisiae 853665 NP_012708.1 CDS YRA2 NC_001143.7 31083 31694 R Yra2p; Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus complement(31083..31694) Saccharomyces cerevisiae 853666 NP_012709.1 CDS DOA1 NC_001143.7 31961 34108 R WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; Doa1p complement(31961..34108) Saccharomyces cerevisiae 853667 NP_012710.1 CDS SAC1 NC_001143.7 34544 36415 D Sac1p; Phosphatidylinositol (PI) phosphatase, involved in hydrolysis of PI 3-phosphate, PI 4-phosphate and PI 3,5-bisphosphate to PI; membrane protein localizes to ER and Golgi; involved in protein trafficking, secretion and inositol metabolism 34544..36415 Saccharomyces cerevisiae 853668 NP_012711.1 CDS TRP3 NC_001143.7 36700 38154 R Trp3p; Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p complement(36700..38154) Saccharomyces cerevisiae 853669 NP_012712.1 CDS UBA1 NC_001143.7 39164 42238 D Ubiquitin activating enzyme (E1), involved in ubiquitin-mediated protein degradation and essential for viability; Uba1p 39164..42238 Saccharomyces cerevisiae 853670 NP_012713.1 CDS STE6 NC_001143.7 42424 46296 R Ste6p; Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells complement(42424..46296) Saccharomyces cerevisiae 853671 NP_012714.1 CDS CBT1 NC_001143.7 47158 47973 D Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p; Cbt1p 47158..47973 Saccharomyces cerevisiae 853673 NP_012715.3 CDS LRC3 NC_001143.7 48195 48956 D Putative protein of unknown function; non-essential gene; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Lrc3p 48195..48956 Saccharomyces cerevisiae 853628 NP_012716.1 CDS ADD66 NC_001143.7 49007 49810 R Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly; Add66p complement(49007..49810) Saccharomyces cerevisiae 853629 NP_012717.1 CDS LOS1 NC_001143.7 50052 53354 D Nuclear pore protein involved in nuclear export of pre-tRNA; Los1p 50052..53354 Saccharomyces cerevisiae 853630 NP_012718.1 CDS EAP1 NC_001143.7 53705 55603 D eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade; Eap1p 53705..55603 Saccharomyces cerevisiae 853631 NP_012719.2 CDS TOR2 NC_001143.7 55936 63360 R Tor2p; PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis complement(55936..63360) Saccharomyces cerevisiae 853632 NP_012721.1 CDS MNN4 NC_001143.7 63931 67467 R Putative positive regulator of mannosylphosphate transferase (Mnn6p), involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; Mnn4p complement(63931..67467) Saccharomyces cerevisiae 853634 NP_012723.2 CDS PTK1 NC_001143.7 68274 70223 R Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein; Ptk1p complement(68274..70223) Saccharomyces cerevisiae 853635 NP_012724.1 CDS PEX1 NC_001143.7 70739 73870 R Pex1p; AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis complement(70739..73870) Saccharomyces cerevisiae 853636 NP_012725.1 CDS YKT6 NC_001143.7 74937 75539 R Ykt6p; Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus complement(74937..75539) Saccharomyces cerevisiae 853638 NP_012726.2 CDS MIA40 NC_001143.7 75826 77037 D Mia40p; Essential protein of the mitochondrial intermembrane space (IMS); promotes retention of newly imported proteins; may do so by stabilizing client protein folding as part of a disulfide relay system or transferring metal to client proteins 75826..77037 Saccharomyces cerevisiae 853639 NP_012727.1 CDS MST1 NC_001143.7 77263 78651 R Mst1p; Mitochondrial threonyl-tRNA synthetase complement(77263..78651) Saccharomyces cerevisiae 853640 NP_012728.1 CDS SDS22 NC_001143.7 78871 79887 R Sds22p; Conserved nuclear regulatory subunit of Glc7p type 1 protein serine-threonine phosphatase (PP1), functions positively with Glc7p to promote dephosphorylation of nuclear substrates required for chromosome transmission during mitosis complement(78871..79887) Saccharomyces cerevisiae 853641 NP_012729.1 CDS ACP1 NC_001143.7 80165 80542 R Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p; Acp1p complement(80165..80542) Saccharomyces cerevisiae 853642 NP_012730.1 CDS DPH2 NC_001143.7 81040 82644 D Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p; Dph2p 81040..82644 Saccharomyces cerevisiae 853643 NP_012731.1 CDS CNB1 NC_001143.7 82952 83555 D Cnb1p; Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 join(82952..83003,83080..83555) Saccharomyces cerevisiae 853644 NP_012732.1 CDS HYM1 NC_001143.7 84709 85908 D Hym1p; Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response 84709..85908 Saccharomyces cerevisiae 853646 NP_012733.1 CDS PXA2 NC_001143.7 86230 88791 R Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins; Pxa2p complement(86230..88791) Saccharomyces cerevisiae 853647 NP_012734.1 CDS YKL187C NC_001143.7 89289 91541 R Ykl187cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies complement(89289..91541) Saccharomyces cerevisiae 853648 NP_012735.1 CDS MTR2 NC_001143.7 92749 93303 R mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA; Mtr2p complement(92749..93303) Saccharomyces cerevisiae 853649 NP_012736.1 CDS ASH1 NC_001143.7 94504 96270 D Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate; Ash1p 94504..96270 Saccharomyces cerevisiae 853650 NP_012737.1 CDS SPE1 NC_001143.7 96762 98162 D Spe1p; Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines 96762..98162 Saccharomyces cerevisiae 853651 NP_878109.1 CDS YKL183C-A NC_001143.7 98400 98612 R Ykl183c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(98400..98612) Saccharomyces cerevisiae 1500485 NP_012738.1 CDS LOT5 NC_001143.7 98726 99646 D Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; Lot5p 98726..99646 Saccharomyces cerevisiae 853652 NP_012739.1 CDS FAS1 NC_001143.7 100676 106831 D Fas1p; Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities 100676..106831 Saccharomyces cerevisiae 853653 NP_012740.1 CDS PRS1 NC_001143.7 107321 108604 D Prs1p; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes 107321..108604 Saccharomyces cerevisiae 853654 NP_012741.1 CDS RPL17A NC_001143.7 109274 110134 D Rpl17ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex) join(109274..109582,109889..110134) Saccharomyces cerevisiae 853674 NP_012742.1 CDS COY1 NC_001143.7 110469 112508 R Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function; Coy1p complement(110469..112508) Saccharomyces cerevisiae 853675 NP_012743.1 CDS STE3 NC_001143.7 113220 114632 R Ste3p; Receptor for a factor receptor, transcribed in alpha cells and required for mating by alpha cells, couples to MAP kinase cascade to mediate pheromone response; ligand bound receptors are endocytosed and recycled to the plasma membrane; GPCR complement(113220..114632) Saccharomyces cerevisiae 853677 NP_012745.1 CDS LST4 NC_001143.7 115505 117991 R Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface; Lst4p complement(115505..117991) Saccharomyces cerevisiae 853678 NP_012746.1 CDS ZRT3 NC_001143.7 118798 120309 D Zrt3p; Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency 118798..120309 Saccharomyces cerevisiae 853679 NP_012747.1 CDS TPO5 NC_001143.7 120385 122241 R Tpo5p; Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles complement(120385..122241) Saccharomyces cerevisiae 853680 NP_012748.1 CDS SNU114 NC_001143.7 122522 125548 D Snu114p; GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 122522..125548 Saccharomyces cerevisiae 853681 NP_012749.1 CDS EBP2 NC_001143.7 125764 127047 D Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles; Ebp2p 125764..127047 Saccharomyces cerevisiae 853682 NP_012750.1 CDS YKL171W NC_001143.7 127480 130266 D Ykl171wp; Putative protein of unknown function; predicted protein kinase; implicated in proteasome function; epitope-tagged protein localizes to the cytoplasm 127480..130266 Saccharomyces cerevisiae 853683 NP_012751.1 CDS MRPL38 NC_001143.7 130639 131055 D Mrpl38p; Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels 130639..131055 Saccharomyces cerevisiae 853684 NP_012753.2 CDS KKQ8 NC_001143.7 131293 133467 R Putative serine/threonine protein kinase with unknown cellular role; Kkq8p complement(131293..133467) Saccharomyces cerevisiae 853686 NP_012754.1 CDS MRP49 NC_001143.7 133726 134139 R Mrp49p; Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation complement(133726..134139) Saccharomyces cerevisiae 853687 NP_012755.1 CDS TPK3 NC_001143.7 134514 135710 R cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk1p and Tpk2p; Tpk3p complement(134514..135710) Saccharomyces cerevisiae 853688 NP_012756.1 CDS MCD4 NC_001143.7 137937 140696 R Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; Mcd4p complement(137937..140696) Saccharomyces cerevisiae 853690 NP_012757.1 CDS PIR1 NC_001143.7 141799 142824 R Pir1p; O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle complement(141799..142824) Saccharomyces cerevisiae 853692 NP_012758.1 CDS PIR3 NC_001143.7 144406 145383 D Pir3p; O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway 144406..145383 Saccharomyces cerevisiae 853693 NP_012760.1 CDS YKL162C NC_001143.7 147635 148843 R Ykl162cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion complement(147635..148843) Saccharomyces cerevisiae 853695 NP_012761.1 CDS YKL161C NC_001143.7 149391 150692 R Ykl161cp; Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; associates with Rlm1p complement(149391..150692) Saccharomyces cerevisiae 853696 NP_012762.1 CDS ELF1 NC_001143.7 153274 153711 D Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression; Elf1p 153274..153711 Saccharomyces cerevisiae 853697 NP_012763.1 CDS RCN1 NC_001143.7 153821 154456 R Rcn1p; Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region complement(153821..154456) Saccharomyces cerevisiae 853698 NP_012765.2 CDS APE2 NC_001143.7 154996 158186 D Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W; Ape2p join(154996..155276,155660..158186) Saccharomyces cerevisiae 853699 NP_012766.1 CDS RPS27A NC_001143.7 158619 159217 D Rps27ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein join(158619..158621,158972..159217) Saccharomyces cerevisiae 853700 NP_012767.1 CDS RSM22 NC_001143.7 159461 161347 R Rsm22p; Mitochondrial ribosomal protein of the small subunit complement(159461..161347) Saccharomyces cerevisiae 853701 NP_012768.1 CDS SRP102 NC_001143.7 161606 162340 D Srp102p; Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors Srp101p to the ER membrane 161606..162340 Saccharomyces cerevisiae 853702 NP_012770.1 CDS GPM1 NC_001143.7 163647 164390 R Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis; Gpm1p complement(163647..164390) Saccharomyces cerevisiae 853705 NP_012771.1 CDS YKL151C NC_001143.7 164922 165935 R Ykl151cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(164922..165935) Saccharomyces cerevisiae 853706 NP_012772.1 CDS MCR1 NC_001143.7 166549 167457 D Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis; Mcr1p 166549..167457 Saccharomyces cerevisiae 853707 NP_012773.1 CDS DBR1 NC_001143.7 167617 168834 R RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition; Dbr1p complement(167617..168834) Saccharomyces cerevisiae 853708 NP_012774.1 CDS SDH1 NC_001143.7 169212 171134 R Sdh1p; Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone complement(169212..171134) Saccharomyces cerevisiae 853709 NP_012776.1 CDS AVT3 NC_001143.7 171788 173866 D Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt3p 171788..173866 Saccharomyces cerevisiae 853710 NP_012777.1 CDS RPT1 NC_001143.7 174218 175621 D Rpt1p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p 174218..175621 Saccharomyces cerevisiae 853712 NP_012778.1 CDS RPC25 NC_001143.7 175848 176486 R Rpc25p; RNA polymerase III subunit C25 complement(175848..176486) Saccharomyces cerevisiae 853713 NP_012779.1 CDS LTV1 NC_001143.7 176786 178177 D Ltv1p; Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature 176786..178177 Saccharomyces cerevisiae 853714 NP_012780.1 CDS MRP8 NC_001143.7 178520 179179 D Putative mitochondrial ribosomal protein, has similarity to E. coli ribosomal protein S2; Mrp8p 178520..179179 Saccharomyces cerevisiae 853715 NP_012781.1 CDS SDH3 NC_001143.7 179672 180268 D Sdh3p; Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone 179672..180268 Saccharomyces cerevisiae 853716 NP_012782.1 CDS TGL1 NC_001143.7 180784 182430 D Tgl1p; Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes 180784..182430 Saccharomyces cerevisiae 853717 NP_012783.1 CDS CTK1 NC_001143.7 182963 184549 D Ctk1p; Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing 182963..184549 Saccharomyces cerevisiae 853718 NP_690844.1 CDS HSK3 NC_001143.7 184808 185017 R Hsk3p; Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis complement(184808..185017) Saccharomyces cerevisiae 853719 NP_012784.1 CDS MRPL31 NC_001143.7 185291 185686 R Mrpl31p; Mitochondrial ribosomal protein of the large subunit complement(185291..185686) Saccharomyces cerevisiae 853720 NP_012785.2 CDS CMC1 NC_001143.7 185962 186297 D Putative protein of unknown function; mutation results in growth defect on a non-fermentable (respiratory) carbon source; Cmc1p 185962..186297 Saccharomyces cerevisiae 853721 NP_012787.1 CDS APL2 NC_001143.7 186465 188645 R Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; Apl2p complement(186465..188645) Saccharomyces cerevisiae 853723 NP_012788.1 CDS OCT1 NC_001143.7 189129 191441 R Oct1p; Mitochondrial intermediate peptidase, cleaves N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis complement(189129..191441) Saccharomyces cerevisiae 853724 NP_012789.1 CDS YKL133C NC_001143.7 191678 193069 R Ykl133cp; Putative protein of unknown function complement(191678..193069) Saccharomyces cerevisiae 853725 NP_012790.1 CDS RMA1 NC_001143.7 193573 194865 R Rma1p; Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(193573..194865) Saccharomyces cerevisiae 853727 NP_012792.1 CDS SHE2 NC_001143.7 195287 196027 R She2p; RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud complement(195287..196027) Saccharomyces cerevisiae 853728 NP_012793.1 CDS MYO3 NC_001143.7 196348 200163 R One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; Myo3p complement(196348..200163) Saccharomyces cerevisiae 853729 NP_012794.1 CDS PMU1 NC_001143.7 200528 201415 R Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant; Pmu1p complement(200528..201415) Saccharomyces cerevisiae 853730 NP_012795.1 CDS PGM1 NC_001143.7 203185 204897 D Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; Pgm1p 203185..204897 Saccharomyces cerevisiae 853732 NP_012796.1 CDS YPK1 NC_001143.7 205351 207393 D Ypk1p; Serine/threonine protein kinase required for receptor-mediated endocytosis; involved in sphingolipid-mediated and cell integrity signaling pathways; localized to the bud neck, cytosol and plasma membrane; homolog of mammalian kinase SGK 205351..207393 Saccharomyces cerevisiae 853733 NP_012797.1 CDS RRN3 NC_001143.7 207891 209774 D Rrn3p; Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA 207891..209774 Saccharomyces cerevisiae 853734 NP_012798.1 CDS SSH4 NC_001143.7 210237 211976 D Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; Ssh4p 210237..211976 Saccharomyces cerevisiae 853735 NP_012800.1 CDS SRP21 NC_001143.7 212139 212642 R Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm; Srp21p complement(212139..212642) Saccharomyces cerevisiae 853737 NP_012801.1 CDS YKL121W NC_001143.7 213786 216344 D Ykl121wp; Putative protein of unknown function 213786..216344 Saccharomyces cerevisiae 853738 NP_012802.1 CDS OAC1 NC_001143.7 216988 217962 D Oac1p; Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, and thiosulfate; member of the mitochondrial carrier family 216988..217962 Saccharomyces cerevisiae 853739 NP_012803.1 CDS VPH2 NC_001143.7 218214 218861 R Vph2p; Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) complement(218214..218861) Saccharomyces cerevisiae 853741 NP_012805.1 CDS SBA1 NC_001143.7 219968 220618 D Sba1p; Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins and like p23 can regulate telomerase activity 219968..220618 Saccharomyces cerevisiae 853743 NP_012806.1 CDS PRR1 NC_001143.7 220988 222544 R Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; Prr1p complement(220988..222544) Saccharomyces cerevisiae 853744 NP_012808.1 CDS APN1 NC_001143.7 222996 224099 R Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; Apn1p complement(222996..224099) Saccharomyces cerevisiae 853746 NP_012809.1 CDS RAD27 NC_001143.7 224371 225519 R Rad27p; 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family complement(224371..225519) Saccharomyces cerevisiae 853747 NP_012810.1 CDS ABF1 NC_001143.7 226214 228409 D DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair; Abf1p 226214..228409 Saccharomyces cerevisiae 853748 NP_012812.1 CDS KTI12 NC_001143.7 228583 229524 R Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p; Kti12p complement(228583..229524) Saccharomyces cerevisiae 853750 NP_012813.1 CDS HAP4 NC_001143.7 231871 233535 D Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; Hap4p 231871..233535 Saccharomyces cerevisiae 853751 NP_012814.1 CDS SLD2 NC_001143.7 234070 235431 D Sld2p; Protein required for DNA replication, phosphorylated in S phase by S-phase cyclin-dependent kinases (Cdks), phosphorylation is essential for DNA replication and for complex formation with Dpb11p; potential Cdc28p substrate 234070..235431 Saccharomyces cerevisiae 853752 NP_012815.1 CDS YKL107W NC_001143.7 235785 236714 D Ykl107wp; Putative protein of unknown function 235785..236714 Saccharomyces cerevisiae 853753 NP_076898.1 CDS YKL106C-A NC_001143.7 236791 236910 R Ykl106c-ap; Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi complement(236791..236910) Saccharomyces cerevisiae 853754 NP_012816.1 CDS AAT1 NC_001143.7 237180 238535 D Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis; Aat1p 237180..238535 Saccharomyces cerevisiae 853755 NP_012817.1 CDS YKL105C NC_001143.7 238829 242227 R Ykl105cp; Putative protein of unknown function complement(238829..242227) Saccharomyces cerevisiae 853756 NP_012818.1 CDS GFA1 NC_001143.7 242864 245017 R Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; Gfa1p complement(242864..245017) Saccharomyces cerevisiae 853757 NP_012819.1 CDS LAP4 NC_001143.7 245782 247326 R Vacuolar aminopeptidase, often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; Lap4p complement(245782..247326) Saccharomyces cerevisiae 853758 NP_012821.1 CDS HSL1 NC_001143.7 248564 253120 D Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p; Hsl1p 248564..253120 Saccharomyces cerevisiae 853760 NP_012822.1 CDS YKL100C NC_001143.7 253341 255104 R Ykl100cp; Putative protein of unknown function with similarity to a human minor histocompatibility antigen; YKL100C is not an essential gene complement(253341..255104) Saccharomyces cerevisiae 853761 NP_012823.2 CDS UTP11 NC_001143.7 255364 256116 R Utp11p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(255364..256116) Saccharomyces cerevisiae 853762 NP_012824.1 CDS YKL098W NC_001143.7 256414 257487 D Ykl098wp; Putative protein of unknown function 256414..257487 Saccharomyces cerevisiae 853763 NP_878110.1 CDS YKL096C-B NC_001143.7 258717 258866 R Ykl096c-bp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(258717..258866) Saccharomyces cerevisiae 1500486 NP_012826.1 CDS CWP2 NC_001143.7 258897 259175 D Cwp2p; Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored 258897..259175 Saccharomyces cerevisiae 853765 NP_012827.1 CDS CWP1 NC_001143.7 260776 261495 D Cwp1p; Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization 260776..261495 Saccharomyces cerevisiae 853766 NP_012828.1 CDS YJU2 NC_001143.7 261921 262757 D Yju2p; Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ('nineteen complex') and acts after Prp2p to promote the first catalytic reaction of splicing 261921..262757 Saccharomyces cerevisiae 853767 NP_012829.1 CDS YJU3 NC_001143.7 262993 263934 D Yju3p; Serine hydrolase with sequence similarity to monoglyceride lipase (MGL), localizes to lipid particles 262993..263934 Saccharomyces cerevisiae 853768 NP_012830.1 CDS MBR1 NC_001143.7 264433 265452 D Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; Mbr1p 264433..265452 Saccharomyces cerevisiae 853769 NP_012831.1 CDS BUD2 NC_001143.7 265789 269103 R GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; Bud2p complement(265789..269103) Saccharomyces cerevisiae 853770 NP_012832.1 CDS YKL091C NC_001143.7 269362 270294 R Ykl091cp; Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus complement(269362..270294) Saccharomyces cerevisiae 853771 NP_012833.1 CDS CUE2 NC_001143.7 271522 272853 D Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination; Cue2p 271522..272853 Saccharomyces cerevisiae 853772 NP_012834.1 CDS MIF2 NC_001143.7 273038 274687 D Mif2p; Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p 273038..274687 Saccharomyces cerevisiae 853773 NP_012835.1 CDS YKL088W NC_001143.7 274927 276642 D Ykl088wp; Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p 274927..276642 Saccharomyces cerevisiae 853774 NP_012836.1 CDS CYT2 NC_001143.7 276833 277507 R Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1; Cyt2p complement(276833..277507) Saccharomyces cerevisiae 853775 NP_012837.1 CDS SRX1 NC_001143.7 277925 278308 D Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxins Tsa1p and Ahp1p that are formed upon exposure to oxidants; conserved in higher eukaryotes; Srx1p 277925..278308 Saccharomyces cerevisiae 853776 NP_012838.1 CDS MDH1 NC_001143.7 278767 279771 D Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated; Mdh1p 278767..279771 Saccharomyces cerevisiae 853777 NP_012839.1 CDS HOT13 NC_001143.7 280153 280503 D Mitochondrial intermembrane space protein, first component of a pathway mediating assembly of small TIM (Translocase of the Inner Membrane) complexes which escort hydrophobic inner membrane proteins en route to the TIM22 complex; Hot13p 280153..280503 Saccharomyces cerevisiae 853778 NP_012841.1 CDS RRP14 NC_001143.7 280669 281973 R Rrp14p; Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family complement(280669..281973) Saccharomyces cerevisiae 853780 NP_012842.1 CDS TEF4 NC_001143.7 282535 284099 D Translation elongation factor EF-1 gamma; Tef4p join(282535..282739,283066..284099) Saccharomyces cerevisiae 853781 NP_012843.1 CDS VMA5 NC_001143.7 284674 285852 D Vma5p; Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane 284674..285852 Saccharomyces cerevisiae 853782 NP_012844.1 CDS SMY1 NC_001143.7 286247 288217 D Smy1p; Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins 286247..288217 Saccharomyces cerevisiae 853783 NP_012845.1 CDS DHR2 NC_001143.7 288489 290696 D Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis; Dhr2p 288489..290696 Saccharomyces cerevisiae 853784 NP_012846.1 CDS YKL077W NC_001143.7 291097 292275 D Ykl077wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole 291097..292275 Saccharomyces cerevisiae 853785 NP_012848.1 CDS YKL075C NC_001143.7 292600 293952 R Ykl075cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin complement(292600..293952) Saccharomyces cerevisiae 853787 NP_012849.1 CDS MUD2 NC_001143.7 294254 295837 R Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65; Mud2p complement(294254..295837) Saccharomyces cerevisiae 853788 NP_012850.1 CDS LHS1 NC_001143.7 296074 298719 D Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway; Lhs1p 296074..298719 Saccharomyces cerevisiae 853789 NP_012851.1 CDS STB6 NC_001143.7 299226 301526 D Stb6p; Protein that binds Sin3p in a two-hybrid assay 299226..301526 Saccharomyces cerevisiae 853790 NP_012852.1 CDS YKL071W NC_001143.7 304758 305528 D Ykl071wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 304758..305528 Saccharomyces cerevisiae 853792 NP_012853.1 CDS YKL070W NC_001143.7 305855 306364 D Ykl070wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 305855..306364 Saccharomyces cerevisiae 853793 NP_012854.1 CDS YKL069W NC_001143.7 306929 307471 D Ykl069wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 306929..307471 Saccharomyces cerevisiae 853794 NP_878111.1 CDS YKL068W-A NC_001143.7 308850 309086 D Ykl068w-ap; Putative protein of unknown function; identified by homology to Ashbya gossypii 308850..309086 Saccharomyces cerevisiae 1500487 NP_012855.1 CDS NUP100 NC_001143.7 309843 312722 D Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup116p; Nup100p 309843..312722 Saccharomyces cerevisiae 853796 NP_012856.1 CDS YNK1 NC_001143.7 314456 314917 D Ynk1p; Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate 314456..314917 Saccharomyces cerevisiae 853798 NP_001032580.1 CDS YKL065W-A NC_001143.7 315598 315819 D Ykl065w-ap; Putative protein of unknown function 315598..315819 Saccharomyces cerevisiae 3799975 NP_012858.1 CDS YET1 NC_001143.7 316081 316701 R Yet1p; Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein complement(316081..316701) Saccharomyces cerevisiae 853800 NP_012859.1 CDS MNR2 NC_001143.7 317408 320317 D Putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; Mnr2p 317408..320317 Saccharomyces cerevisiae 853801 NP_012860.1 CDS YKL063C NC_001143.7 320659 321162 R Ykl063cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi complement(320659..321162) Saccharomyces cerevisiae 853802 NP_012861.1 CDS MSN4 NC_001143.7 322872 324764 D Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; Msn4p 322872..324764 Saccharomyces cerevisiae 853803 NP_012862.1 CDS YKL061W NC_001143.7 325415 325756 D Ykl061wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome 325415..325756 Saccharomyces cerevisiae 853804 NP_012863.1 CDS FBA1 NC_001143.7 326052 327131 R Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; Fba1p complement(326052..327131) Saccharomyces cerevisiae 853805 NP_012864.1 CDS MPE1 NC_001143.7 327762 329087 R Mpe1p; Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif complement(327762..329087) Saccharomyces cerevisiae 853806 NP_012865.1 CDS TOA2 NC_001143.7 329810 330178 D Toa2p; TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA 329810..330178 Saccharomyces cerevisiae 853807 NP_012866.1 CDS NUP120 NC_001143.7 330500 333613 R Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; Nup120p complement(330500..333613) Saccharomyces cerevisiae 853808 NP_012867.1 CDS TMA19 NC_001143.7 334056 334559 R Tma19p; Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress complement(334056..334559) Saccharomyces cerevisiae 853809 NP_012868.1 CDS OAR1 NC_001143.7 334965 335801 R Oar1p; Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p complement(334965..335801) Saccharomyces cerevisiae 853810 NP_012869.1 CDS DEF1 NC_001143.7 336181 338397 R RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; Def1p complement(336181..338397) Saccharomyces cerevisiae 853811 NP_012870.1 CDS MDM35 NC_001143.7 338826 339086 R Mitochondrial intermembrane space cysteine motif protein; mutation affects mitochondrial distribution and morphology; Mdm35p complement(338826..339086) Saccharomyces cerevisiae 853812 NP_012872.1 CDS ASK1 NC_001143.7 339312 340190 R Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; Ask1p complement(339312..340190) Saccharomyces cerevisiae 853814 NP_012873.1 CDS SFK1 NC_001143.7 340956 342017 D Sfk1p; Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane 340956..342017 Saccharomyces cerevisiae 853815 NP_012874.1 CDS YKL050C NC_001143.7 342496 345264 R Ykl050cp; Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p complement(342496..345264) Saccharomyces cerevisiae 853816 NP_012875.2 CDS CSE4 NC_001143.7 345719 346408 R Cse4p; Centromere protein that resembles histones, required for proper kinetochore function; homolog of human CENP-A complement(345719..346408) Saccharomyces cerevisiae 853817 NP_012876.1 CDS ELM1 NC_001143.7 346858 348780 R Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring; Elm1p complement(346858..348780) Saccharomyces cerevisiae 853818 NP_012877.1 CDS YKL047W NC_001143.7 349107 350657 D Ykl047wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 349107..350657 Saccharomyces cerevisiae 853819 NP_012878.1 CDS DCW1 NC_001143.7 350919 352268 R Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p; Dcw1p complement(350919..352268) Saccharomyces cerevisiae 853820 NP_012879.1 CDS PRI2 NC_001143.7 353137 354723 D Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair; Pri2p 353137..354723 Saccharomyces cerevisiae 853821 NP_012881.1 CDS PHD1 NC_001143.7 356392 357492 D Transcriptional activator that enhances pseudohyphal growth; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; Phd1p 356392..357492 Saccharomyces cerevisiae 853823 NP_012882.1 CDS SPC42 NC_001143.7 358119 359210 D Spc42p; Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane 358119..359210 Saccharomyces cerevisiae 853824 NP_012883.1 CDS VPS24 NC_001143.7 359786 360460 D Vps24p; One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway 359786..360460 Saccharomyces cerevisiae 853825 NP_012884.1 CDS NFU1 NC_001143.7 360701 361471 R Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria; Nfu1p complement(360701..361471) Saccharomyces cerevisiae 853826 NP_012885.2 CDS PTM1 NC_001143.7 362265 363836 D Ptm1p; Protein of unknown function, copurifies with late Golgi vesicles containing the v-SNARE Tlg2p 362265..363836 Saccharomyces cerevisiae 853827 NP_012886.1 CDS RGT1 NC_001143.7 365248 368760 D Rgt1p; Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor 365248..368760 Saccharomyces cerevisiae 853828 NP_012887.1 CDS AIM26 NC_001143.7 369008 369364 D Putative protein of unknown function; null mutant is viable and displays increased frequency of mitochondrial genome loss (petite formation); Aim26p 369008..369364 Saccharomyces cerevisiae 853829 NP_012889.1 CDS UGP1 NC_001143.7 369534 371033 D Ugp1p; UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p 369534..371033 Saccharomyces cerevisiae 853830 NP_012890.1 CDS TUL1 NC_001143.7 371472 373748 D Tul1p; Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes 371472..373748 Saccharomyces cerevisiae 853832 NP_012891.2 CDS YKL033W-A NC_001143.7 374148 374858 D Ykl033w-ap; Putative protein of unknown function; similar to uncharacterized proteins from other fungi 374148..374858 Saccharomyces cerevisiae 853833 NP_012892.1 CDS YKL033W NC_001143.7 375099 378215 D Ykl033wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YKL033W is an essential gene 375099..378215 Saccharomyces cerevisiae 853834 NP_012893.1 CDS IXR1 NC_001143.7 379708 381501 R Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b; Ixr1p complement(379708..381501) Saccharomyces cerevisiae 853836 NP_012896.1 CDS MAE1 NC_001143.7 382359 384368 R Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids; Mae1p complement(382359..384368) Saccharomyces cerevisiae 853839 NP_012897.1 CDS TFA1 NC_001143.7 385401 386849 D Tfa1p; TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening 385401..386849 Saccharomyces cerevisiae 853840 NP_012898.1 CDS YKL027W NC_001143.7 387205 388548 D Ykl027wp; Protein of unknown function, localized to the mitochondrial outer membrane 387205..388548 Saccharomyces cerevisiae 853841 NP_012899.1 CDS GPX1 NC_001143.7 389023 389526 R Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; Gpx1p complement(389023..389526) Saccharomyces cerevisiae 853842 NP_012900.1 CDS PAN3 NC_001143.7 389883 391922 R Pan3p; Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes complement(389883..391922) Saccharomyces cerevisiae 853843 NP_012901.1 CDS URA6 NC_001143.7 392169 392783 R Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP); Ura6p complement(392169..392783) Saccharomyces cerevisiae 853844 NP_001032581.1 CDS YKL023C-A NC_001143.7 392789 393016 R Ykl023c-ap; Putative protein of unknown function complement(392789..393016) Saccharomyces cerevisiae 3799976 NP_012902.1 CDS YKL023W NC_001143.7 393364 394197 D Ykl023wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 393364..394197 Saccharomyces cerevisiae 853845 NP_012903.1 CDS CDC16 NC_001143.7 394369 396891 R Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; Cdc16p complement(394369..396891) Saccharomyces cerevisiae 853846 NP_012904.1 CDS MAK11 NC_001143.7 396987 398393 R Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats; Mak11p complement(396987..398393) Saccharomyces cerevisiae 853847 NP_012905.1 CDS SPT23 NC_001143.7 398475 401723 R Spt23p; ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting complement(398475..401723) Saccharomyces cerevisiae 853848 NP_012906.1 CDS RAM2 NC_001143.7 402211 403161 D Ram2p; Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor 402211..403161 Saccharomyces cerevisiae 853849 NP_076899.1 CDS YKL018C-A NC_001143.7 403223 403522 R Ykl018c-ap; Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(403223..403522) Saccharomyces cerevisiae 853850 NP_012907.1 CDS SWD2 NC_001143.7 403745 404734 D Swd2p; Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination 403745..404734 Saccharomyces cerevisiae 853851 NP_012908.1 CDS HCS1 NC_001143.7 404833 406884 R Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities; Hcs1p complement(404833..406884) Saccharomyces cerevisiae 853852 NP_012909.1 CDS ATP7 NC_001143.7 407108 407632 R Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp7p complement(407108..407632) Saccharomyces cerevisiae 853853 NP_012910.1 CDS PUT3 NC_001143.7 408187 411126 D Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences and undergoes a conformational change to form the active state; has a Zn(2)-Cys(6) binuclear cluster domain; Put3p 408187..411126 Saccharomyces cerevisiae 853854 NP_012911.1 CDS URB1 NC_001143.7 411262 416556 R Urb1p; Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit complement(411262..416556) Saccharomyces cerevisiae 853855 NP_012912.1 CDS ARC19 NC_001143.7 417151 417666 R Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; Arc19p complement(417151..417666) Saccharomyces cerevisiae 853856 NP_012913.1 CDS PRP40 NC_001143.7 417953 419704 D U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex; Prp40p 417953..419704 Saccharomyces cerevisiae 853857 NP_012914.1 CDS CCE1 NC_001143.7 419798 420859 R Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA; Cce1p complement(419798..420859) Saccharomyces cerevisiae 853858 NP_012915.1 CDS UFD4 NC_001143.7 421067 425518 R Ufd4p; Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins complement(421067..425518) Saccharomyces cerevisiae 853859 NP_012916.1 CDS MRT4 NC_001143.7 425885 426595 D Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus; Mrt4p 425885..426595 Saccharomyces cerevisiae 853860 NP_012917.1 CDS LAC1 NC_001143.7 426938 428194 R Lac1p; Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p complement(426938..428194) Saccharomyces cerevisiae 853861 NP_012918.1 CDS CAP1 NC_001143.7 428945 429751 D Cap1p; Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches 428945..429751 Saccharomyces cerevisiae 853862 NP_012919.1 CDS SFT1 NC_001143.7 429841 430275 R Sft1p; Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment complement(join(429841..430098,430240..430275)) Saccharomyces cerevisiae 853863 NP_012920.1 CDS RPL14A NC_001143.7 431549 432363 D Rpl14ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth join(431549..431677,432076..432363) Saccharomyces cerevisiae 853864 NP_012921.1 CDS BYE1 NC_001143.7 432736 434520 R Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit; Bye1p complement(432736..434520) Saccharomyces cerevisiae 853865 NP_012922.1 CDS AUR1 NC_001143.7 435223 436428 D Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; Aur1p 435223..436428 Saccharomyces cerevisiae 853866 NP_012923.1 CDS MRP17 NC_001143.7 436740 437135 R Mrp17p; Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator complement(436740..437135) Saccharomyces cerevisiae 853867 NP_012924.2 CDS DID4 NC_001143.7 437421 438187 D Did4p; Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis join(437421..437480,437549..438187) Saccharomyces cerevisiae 853868 NP_012925.1 CDS MET14 NC_001143.7 438420 439028 R Met14p; Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism complement(438420..439028) Saccharomyces cerevisiae 853869 NP_012926.1 CDS VPS1 NC_001143.7 440251 442365 R Vps1p; Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, late Golgi-retention of some proteins, regulating peroxisome biogenesis complement(440251..442365) Saccharomyces cerevisiae 853870 NP_012927.1 CDS PAP1 NC_001143.7 442875 444581 D Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; Pap1p 442875..444581 Saccharomyces cerevisiae 853871 NP_012928.1 CDS OSH6 NC_001143.7 445024 446370 D Osh6p; Member of an oxysterol-binding protein family with overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery 445024..446370 Saccharomyces cerevisiae 853872 NP_012929.2 CDS ECM9 NC_001143.7 446442 447679 R Non-essential protein of unknown function; Ecm9p complement(join(446442..447349,447454..447679)) Saccharomyces cerevisiae 853873 NP_012930.2 CDS YKR005C NC_001143.7 448164 449810 R Ykr005cp; Putative protein of unknown function complement(join(448164..449594,449664..449810)) Saccharomyces cerevisiae 853874 NP_012931.2 CDS MRPL13 NC_001143.7 450066 450860 R Mrpl13p; Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation complement(450066..450860) Saccharomyces cerevisiae 853875 NP_012932.1 CDS MEH1 NC_001143.7 451077 451631 D Meh1p; Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification 451077..451631 Saccharomyces cerevisiae 853876 NP_012933.1 CDS RSC4 NC_001143.7 451844 453721 D Rsc4p; Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling 451844..453721 Saccharomyces cerevisiae 853877 NP_012934.1 CDS FOX2 NC_001143.7 453995 456697 R Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities; Fox2p complement(453995..456697) Saccharomyces cerevisiae 853878 NP_012935.1 CDS TOF2 NC_001143.7 458567 460882 R Tof2p; Nonessential mitochondrial protein of unknown function with sequence similarity to Net1p; identified as a topoisomerase I (Top1p) binding protein; displays synthetic genetic interactions with TOP1 and HPR1 complement(458567..460882) Saccharomyces cerevisiae 853880 NP_012936.1 CDS YKR011C NC_001143.7 461278 462339 R Ykr011cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus complement(461278..462339) Saccharomyces cerevisiae 853881 NP_012938.1 CDS PRY2 NC_001143.7 463602 464591 D Pry2p; Protein of unknown function, has similarity to Pry1p and Pry3p and to the plant PR-1 class of pathogen related proteins 463602..464591 Saccharomyces cerevisiae 853882 NP_012939.1 CDS YPT52 NC_001143.7 465010 465714 R Ypt52p; GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis complement(465010..465714) Saccharomyces cerevisiae 853884 NP_012940.1 CDS YKR015C NC_001143.7 466604 468310 R Ykr015cp; Putative protein of unknown function complement(466604..468310) Saccharomyces cerevisiae 853885 NP_012941.1 CDS AIM28 NC_001143.7 469360 470982 D Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss and severe growth defect in minimal glycerol media; Aim28p 469360..470982 Saccharomyces cerevisiae 853886 NP_012942.1 CDS YKR017C NC_001143.7 471337 472992 R Ykr017cp; Putative protein of unknown function; contains a RING finger motif complement(471337..472992) Saccharomyces cerevisiae 853887 NP_012943.1 CDS YKR018C NC_001143.7 473366 475543 R Ykr018cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(473366..475543) Saccharomyces cerevisiae 853888 NP_012944.1 CDS IRS4 NC_001143.7 475859 477706 R Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p; mutation confers an increase in rDNA silencing; Irs4p complement(475859..477706) Saccharomyces cerevisiae 853889 NP_012945.1 CDS VPS51 NC_001143.7 477981 478475 D Vps51p; Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p 477981..478475 Saccharomyces cerevisiae 853890 NP_012946.1 CDS ALY1 NC_001143.7 478877 481624 D Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; Aly1p 478877..481624 Saccharomyces cerevisiae 853891 NP_012947.1 CDS NTR2 NC_001143.7 481814 482782 R Ntr2p; Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly complement(481814..482782) Saccharomyces cerevisiae 853892 NP_012948.1 CDS YKR023W NC_001143.7 483062 484654 D Ykr023wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 483062..484654 Saccharomyces cerevisiae 853893 NP_012949.1 CDS DBP7 NC_001143.7 484787 487015 R Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; Dbp7p complement(484787..487015) Saccharomyces cerevisiae 853894 NP_012950.1 CDS RPC37 NC_001143.7 487413 488261 D Rpc37p; RNA polymerase III subunit C37 487413..488261 Saccharomyces cerevisiae 853895 NP_012951.1 CDS GCN3 NC_001143.7 488381 489298 R Gcn3p; Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression complement(488381..489298) Saccharomyces cerevisiae 853896 NP_012952.1 CDS BCH2 NC_001143.7 491007 493304 D Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; Bch2p 491007..493304 Saccharomyces cerevisiae 853898 NP_012953.1 CDS SAP190 NC_001143.7 493900 497196 D Sap190p; Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p 493900..497196 Saccharomyces cerevisiae 853899 NP_012954.1 CDS SET3 NC_001143.7 497220 499475 R Set3p; Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains complement(497220..499475) Saccharomyces cerevisiae 853900 NP_012955.1 CDS GMH1 NC_001143.7 499924 500745 D Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting; Gmh1p 499924..500745 Saccharomyces cerevisiae 853901 NP_012956.1 CDS SPO14 NC_001143.7 500986 506037 R Spo14p; Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis complement(500986..506037) Saccharomyces cerevisiae 853902 NP_012959.1 CDS DAL80 NC_001143.7 506540 507349 D Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p; Dal80p 506540..507349 Saccharomyces cerevisiae 853904 NP_012961.1 CDS DID2 NC_001143.7 507581 508195 D Did2p; Class E protein of the vacuolar protein-sorting (Vps) pathway, associates reversibly with the late endosome, has human ortholog that may be altered in breast tumors 507581..508195 Saccharomyces cerevisiae 853906 NP_012962.2 CDS CAF4 NC_001143.7 508344 510275 R WD40 repeat-containing protein associated with the CCR4-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; Caf4p complement(508344..510275) Saccharomyces cerevisiae 853908 NP_012963.1 CDS SPC34 NC_001143.7 510552 511439 R Spc34p; Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body complement(510552..511439) Saccharomyces cerevisiae 853909 NP_012964.2 CDS KAE1 NC_001143.7 511641 512801 R Putative glycoprotease proposed to be in transcription as a component of the EKC protein complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; also identified as a component of the KEOPS protein complex; Kae1p complement(511641..512801) Saccharomyces cerevisiae 853910 NP_012965.1 CDS GAP1 NC_001143.7 514705 516513 D General amino acid permease; localization to the plasma membrane is regulated by nitrogen source; Gap1p 514705..516513 Saccharomyces cerevisiae 853912 NP_012967.1 CDS YKR041W NC_001143.7 517840 518592 D Ykr041wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 517840..518592 Saccharomyces cerevisiae 853914 NP_012968.2 CDS UTH1 NC_001143.7 519169 520266 D Uth1p; Mitochondrial outer membrane and cell wall localized SUN family member required for mitochondrial autophagy; involved in the oxidative stress response, life span during starvation, mitochondrial biogenesis, and cell death 519169..520266 Saccharomyces cerevisiae 853916 NP_012969.1 CDS YKR043C NC_001143.7 520539 521354 R Ykr043cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus complement(520539..521354) Saccharomyces cerevisiae 853917 NP_012970.1 CDS UIP5 NC_001143.7 521657 522988 D Uip5p; Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates 521657..522988 Saccharomyces cerevisiae 853918 NP_012971.2 CDS YKR045C NC_001143.7 523060 523611 R Ykr045cp; Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm complement(523060..523611) Saccharomyces cerevisiae 853919 NP_012972.1 CDS PET10 NC_001143.7 523865 524716 R Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange; Pet10p complement(523865..524716) Saccharomyces cerevisiae 853920 NP_012974.1 CDS NAP1 NC_001143.7 525029 526282 R Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; Nap1p complement(525029..526282) Saccharomyces cerevisiae 853922 NP_012975.1 CDS FMP46 NC_001143.7 526472 526873 R Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp46p complement(526472..526873) Saccharomyces cerevisiae 853923 NP_012976.1 CDS TRK2 NC_001143.7 527457 530126 D Trk2p; Component of the Trk1p-Trk2p potassium transport system 527457..530126 Saccharomyces cerevisiae 853924 NP_012977.1 CDS YKR051W NC_001143.7 530588 531844 D Ykr051wp; Putative protein of unknown function 530588..531844 Saccharomyces cerevisiae 853925 NP_012978.1 CDS MRS4 NC_001143.7 532192 533106 R Mrs4p; Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs3p; functions under low-iron conditions; may transport other cations in addition to iron complement(532192..533106) Saccharomyces cerevisiae 853926 NP_012979.1 CDS YSR3 NC_001143.7 533709 534923 R Ysr3p; Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p complement(533709..534923) Saccharomyces cerevisiae 853927 NP_012980.1 CDS DYN1 NC_001143.7 535289 547567 R Dyn1p; Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p complement(535289..547567) Saccharomyces cerevisiae 853928 NP_012981.1 CDS RHO4 NC_001143.7 547858 548733 D Rho4p; Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity 547858..548733 Saccharomyces cerevisiae 853929 NP_012982.2 CDS TRM2 NC_001143.7 549090 551009 D tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair; Trm2p 549090..551009 Saccharomyces cerevisiae 853930 NP_012983.1 CDS RPS21A NC_001143.7 551299 551884 D Rps21ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Bp and has similarity to rat S21 ribosomal protein join(551299..551322,551645..551884) Saccharomyces cerevisiae 853931 NP_012984.2 CDS GLG1 NC_001143.7 552412 554262 D Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; Glg1p 552412..554262 Saccharomyces cerevisiae 853932 NP_012985.1 CDS TIF1 NC_001143.7 554629 555816 D Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; Tif1p 554629..555816 Saccharomyces cerevisiae 853933 NP_012986.1 CDS UTP30 NC_001143.7 556160 556984 D Utp30p; Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data 556160..556984 Saccharomyces cerevisiae 853934 NP_012987.1 CDS KTR2 NC_001143.7 557319 558596 D Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Ktr2p 557319..558596 Saccharomyces cerevisiae 853935 NP_012988.1 CDS TFA2 NC_001143.7 559308 560294 D Tfa2p; TFIIE small subunit, involved in RNA polymerase II transcription initiation 559308..560294 Saccharomyces cerevisiae 853936 NP_012989.1 CDS LAS1 NC_001143.7 560431 561939 R Las1p; Essential nuclear protein possibly involved in bud formation and morphogenesis; mutants require the SSD1-v allele for viability complement(560431..561939) Saccharomyces cerevisiae 853937 NP_012990.1 CDS OAF3 NC_001143.7 562189 564780 D Zinc cluster protein; regulates transcription in response to oleate levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Oaf3p 562189..564780 Saccharomyces cerevisiae 853938 NP_012991.1 CDS PAM17 NC_001143.7 564941 565534 R Presequence translocase-associated motor subunit, required for stable complex formation between cochaperones Pam16p and Pam18p, promotes association of Pam16p-Pam18p with the presequence translocase; Pam17p complement(564941..565534) Saccharomyces cerevisiae 853939 NP_012992.1 CDS CCP1 NC_001143.7 565755 566840 R Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress; Ccp1p complement(565755..566840) Saccharomyces cerevisiae 853940 NP_012993.1 CDS GPT2 NC_001143.7 567560 569791 D Gpt2p; Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis 567560..569791 Saccharomyces cerevisiae 853941 NP_012994.1 CDS BET3 NC_001143.7 569970 570551 R Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex; Bet3p complement(569970..570551) Saccharomyces cerevisiae 853942 NP_012995.1 CDS MET1 NC_001143.7 571254 573035 D S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis; Met1p 571254..573035 Saccharomyces cerevisiae 853943 NP_012996.1 CDS YKR070W NC_001143.7 573216 574274 D Ykr070wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 573216..574274 Saccharomyces cerevisiae 853944 NP_012997.1 CDS DRE2 NC_001143.7 574576 575622 R Protein of unknown function; mutation displays synthetic lethal interaction with the pol3-13 allele of CDC2; Dre2p complement(574576..575622) Saccharomyces cerevisiae 853945 NP_012998.1 CDS SIS2 NC_001143.7 576077 577765 R Sis2p; Negative regulatory subunit of the protein phosphatase 1 Ppz1p; involved in ion homeostasis and cell cycle progression complement(576077..577765) Saccharomyces cerevisiae 853946 NP_013000.1 CDS AIM29 NC_001143.7 578851 579318 D Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim29p 578851..579318 Saccharomyces cerevisiae 853949 NP_013001.1 CDS YKR075C NC_001143.7 579469 580392 R Ykr075cp; Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS complement(579469..580392) Saccharomyces cerevisiae 853950 NP_013002.1 CDS ECM4 NC_001143.7 581925 583037 D Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; Ecm4p 581925..583037 Saccharomyces cerevisiae 853951 NP_013003.1 CDS MSA2 NC_001143.7 583295 584386 D Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation; Msa2p 583295..584386 Saccharomyces cerevisiae 853952 NP_013004.1 CDS YKR078W NC_001143.7 584594 586351 D Ykr078wp; Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) 584594..586351 Saccharomyces cerevisiae 853953 NP_013005.1 CDS TRZ1 NC_001143.7 586431 588947 R tRNase Z, involved in RNA processing, has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif, homolog of the human candidate prostate cancer susceptibility gene ELAC2; Trz1p complement(586431..588947) Saccharomyces cerevisiae 853954 NP_013006.1 CDS MTD1 NC_001143.7 590037 590999 D NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline; Mtd1p 590037..590999 Saccharomyces cerevisiae 853955 NP_013007.1 CDS RPF2 NC_001143.7 591148 592182 R Rpf2p; Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles complement(591148..592182) Saccharomyces cerevisiae 853956 NP_013008.1 CDS NUP133 NC_001143.7 592467 595940 D Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; Nup133p 592467..595940 Saccharomyces cerevisiae 853957 NP_013009.1 CDS DAD2 NC_001143.7 596063 596464 R Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Dad2p complement(596063..596464) Saccharomyces cerevisiae 853958 NP_013010.1 CDS HBS1 NC_001143.7 596697 598532 R Hbs1p; GTP binding protein with sequence similarity to the elongation factor class of G proteins, EF-1alpha and Sup35p; associates with Dom34p, and shares a similar genetic relationship with genes that encode ribosomal protein components complement(596697..598532) Saccharomyces cerevisiae 853959 NP_013011.1 CDS MRPL20 NC_001143.7 598736 599323 R Mrpl20p; Mitochondrial ribosomal protein of the large subunit complement(598736..599323) Saccharomyces cerevisiae 853960 NP_013012.1 CDS PRP16 NC_001143.7 599499 602714 D RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity; Prp16p 599499..602714 Saccharomyces cerevisiae 853961 NP_013013.2 CDS OMA1 NC_001143.7 602837 603874 R Oma1p; Metalloendopeptidase of the mitochondrial inner membrane, involved in turnover of membrane-embedded proteins; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes complement(602837..603874) Saccharomyces cerevisiae 853962 NP_013014.1 CDS TVP38 NC_001143.7 604047 605060 R Tvp38p; Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(604047..605060) Saccharomyces cerevisiae 853963 NP_013015.1 CDS TGL4 NC_001143.7 605275 608007 R Triacylglycerol lipase involved in triacylglycerol mobilization and degradation; found in lipid particles; potential Cdc28p substrate; Tgl4p complement(605275..608007) Saccharomyces cerevisiae 853964 NP_013016.1 CDS PXL1 NC_001143.7 608588 610708 D Pxl1p; LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin 608588..610708 Saccharomyces cerevisiae 853965 NP_013017.2 CDS SRL3 NC_001143.7 611167 611907 D Srl3p; Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate 611167..611907 Saccharomyces cerevisiae 853966 NP_013018.1 CDS SRP40 NC_001143.7 612307 613527 R Srp40p; Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 complement(612307..613527) Saccharomyces cerevisiae 853967 NP_013019.1 CDS PTR2 NC_001143.7 615372 617177 D Ptr2p; Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p 615372..617177 Saccharomyces cerevisiae 853968 NP_013020.1 CDS RPL40B NC_001143.7 617638 618392 R Rpl40bp; Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes complement(join(617638..618016,618385..618392)) Saccharomyces cerevisiae 853969 NP_013021.1 CDS MLP1 NC_001143.7 619447 625074 D Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; Mlp1p 619447..625074 Saccharomyces cerevisiae 853970 NP_878113.2 CDS PCC1 NC_001143.7 625506 625847 D Proposed transcription factor involved in the expression of genes regulated by alpha-factor and galactose; component of the EKC/KEOPS protein complex with Kae1p, Gon7p, Bud32p, and Cgi121p; related to human cancer-testis antigens; Pcc1p join(625506..625543,625619..625847) Saccharomyces cerevisiae 1500489 NP_013022.1 CDS YKR096W NC_001143.7 626435 630022 D Ykr096wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc (PilT N terminus) domain 626435..630022 Saccharomyces cerevisiae 853971 NP_013023.1 CDS PCK1 NC_001143.7 630790 632439 D Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol; Pck1p 630790..632439 Saccharomyces cerevisiae 853972 NP_013024.1 CDS UBP11 NC_001143.7 632664 634817 R Ubp11p; Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins complement(632664..634817) Saccharomyces cerevisiae 853973 NP_013025.1 CDS BAS1 NC_001143.7 635489 637924 D Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes; Bas1p 635489..637924 Saccharomyces cerevisiae 853974 NP_013026.2 CDS SKG1 NC_001143.7 638539 639606 R Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell; Skg1p complement(638539..639606) Saccharomyces cerevisiae 853975 NP_013027.1 CDS SIR1 NC_001143.7 640106 642142 D Sir1p; Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin 640106..642142 Saccharomyces cerevisiae 853976 NP_013028.1 CDS FLO10 NC_001143.7 645994 649503 D Lectin-like protein with similarity to Flo1p, thought to be involved in flocculation; Flo10p 645994..649503 Saccharomyces cerevisiae 853977 NP_013029.1 CDS NFT1 NC_001143.7 652718 656374 D Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.; Nft1p 652718..656374 Saccharomyces cerevisiae 853978 NP_013030.1 CDS YKR104W NC_001143.7 656474 657394 D Ykr104wp; Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds 656474..657394 Saccharomyces cerevisiae 853979 NP_013031.1 CDS YKR105C NC_001143.7 658354 660102 R Ykr105cp; Putative transporter of the Major Facilitator Superfamily (MFS) complement(658354..660102) Saccharomyces cerevisiae 853980 NP_013032.1 CDS YKR106W NC_001143.7 661080 662927 D Ykr106wp; Protein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YCL073C 661080..662927 Saccharomyces cerevisiae 853981 NP_013033.1 CDS YLL067C NC_001144.4 585 4301 R Yll067cp; Putative protein of unknown function with similarity to helicases complement(join(585..3915,4015..4301)) Saccharomyces cerevisiae 850659 NP_878115.1 CDS YLL066W-B NC_001144.4 5605 5775 D Yll066w-bp; Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data 5605..5775 Saccharomyces cerevisiae 1466404 NP_013034.1 CDS YLL066C NC_001144.4 6120 9836 R Yll066cp; Putative protein of unknown function with similarity to helicases; YLL066C is not an essential gene complement(join(6120..9450,9550..9836)) Saccharomyces cerevisiae 850660 NP_013036.1 CDS PAU18 NC_001144.4 13083 13445 R Pau18p complement(13083..13445) Saccharomyces cerevisiae 850662 NP_013037.1 CDS AYT1 NC_001144.4 14648 16072 R Ayt1p; Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis complement(14648..16072) Saccharomyces cerevisiae 850663 NP_013038.1 CDS MHT1 NC_001144.4 16639 17613 R S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; Mht1p complement(16639..17613) Saccharomyces cerevisiae 850664 NP_013039.1 CDS MMP1 NC_001144.4 17956 19707 D Mmp1p; High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p 17956..19707 Saccharomyces cerevisiae 850665 NP_013040.1 CDS GTT2 NC_001144.4 21138 21839 R Gtt2p; Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p complement(21138..21839) Saccharomyces cerevisiae 850666 NP_013042.1 CDS YLL058W NC_001144.4 23569 25296 D Yll058wp; Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene 23569..25296 Saccharomyces cerevisiae 850668 NP_013043.1 CDS JLP1 NC_001144.4 25756 26994 R Jlp1p; Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) complement(25756..26994) Saccharomyces cerevisiae 850669 NP_013044.1 CDS YLL056C NC_001144.4 27409 28305 R Yll056cp; Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in pleiotropic drug resistance (PDR) phenomenon; YLL056C is not an essential gene complement(27409..28305) Saccharomyces cerevisiae 850670 NP_013045.1 CDS YCT1 NC_001144.4 30109 31704 D Yct1p; High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene 30109..31704 Saccharomyces cerevisiae 850671 NP_013046.1 CDS YLL054C NC_001144.4 32894 35203 R Yll054cp; Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene complement(32894..35203) Saccharomyces cerevisiae 850672 NP_013047.1 CDS YLL053C NC_001144.4 35502 35960 R Yll053cp; Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin complement(35502..35960) Saccharomyces cerevisiae 850673 NP_013048.1 CDS AQY2 NC_001144.4 35911 36360 R Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains; Aqy2p complement(35911..36360) Saccharomyces cerevisiae 850674 NP_013049.1 CDS FRE6 NC_001144.4 37332 39470 R Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; Fre6p complement(37332..39470) Saccharomyces cerevisiae 850675 NP_013050.1 CDS COF1 NC_001144.4 39803 40413 R Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments , thought to be regulated by phosphorylation at SER4, ubiquitous and essential in eukaryotes; Cof1p complement(join(39803..40220,40400..40413)) Saccharomyces cerevisiae 850676 NP_013051.1 CDS LDB18 NC_001144.4 40665 41204 D Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface; Ldb18p 40665..41204 Saccharomyces cerevisiae 850677 NP_013052.1 CDS YBT1 NC_001144.4 41279 46264 R Ybt1p; Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters complement(41279..46264) Saccharomyces cerevisiae 850678 NP_013054.1 CDS RNP1 NC_001144.4 46713 47462 R Rnp1p; Ribonucleoprotein that contains two RNA recognition motifs (RRM) complement(46713..47462) Saccharomyces cerevisiae 850680 NP_013055.1 CDS RPL8B NC_001144.4 47858 48628 R Rpl8bp; Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits complement(47858..48628) Saccharomyces cerevisiae 850682 NP_013057.1 CDS FPS1 NC_001144.4 49937 51946 D Plasma membrane glycerol channel, member of the major intrinsic protein (MIP) family of channel proteins; involved in efflux of glycerol and in uptake of the trivalent metalloids arsenite and antimonite; Fps1p 49937..51946 Saccharomyces cerevisiae 850683 NP_013058.1 CDS ATG10 NC_001144.4 52086 52589 R E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy; Atg10p complement(52086..52589) Saccharomyces cerevisiae 850684 NP_013059.1 CDS SDH2 NC_001144.4 53130 53930 R Sdh2p; Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone complement(53130..53930) Saccharomyces cerevisiae 850685 NP_013060.1 CDS VPS13 NC_001144.4 54210 63644 R Vps13p; Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention complement(54210..63644) Saccharomyces cerevisiae 850619 NP_013061.1 CDS UBI4 NC_001144.4 64061 65206 R Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; Ubi4p complement(64061..65206) Saccharomyces cerevisiae 850620 NP_013062.1 CDS ENT4 NC_001144.4 65774 66517 R Protein of unknown function, contains an N-terminal epsin-like domain; Ent4p complement(65774..66517) Saccharomyces cerevisiae 850621 NP_013064.1 CDS PRP19 NC_001144.4 66744 68255 R Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases; Prp19p complement(66744..68255) Saccharomyces cerevisiae 850623 NP_013065.1 CDS GRC3 NC_001144.4 68579 70477 D Protein of unknown function, required for cell growth and possibly involved in rRNA processing; mRNA is cell cycle regulated; Grc3p 68579..70477 Saccharomyces cerevisiae 850624 NP_013066.1 CDS RIX7 NC_001144.4 70632 73145 R Rix7p; Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions complement(70632..73145) Saccharomyces cerevisiae 850625 NP_013067.1 CDS IRC19 NC_001144.4 73408 74100 D Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52 foci; Irc19p 73408..74100 Saccharomyces cerevisiae 850626 NP_013068.1 CDS YLL032C NC_001144.4 74269 76746 R Yll032cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene complement(74269..76746) Saccharomyces cerevisiae 850627 NP_013069.1 CDS GPI13 NC_001144.4 77151 80204 R Gpi13p; ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein complement(77151..80204) Saccharomyces cerevisiae 850628 NP_013071.1 CDS RUP2 NC_001144.4 81460 83709 D Rup2p; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation 81460..83709 Saccharomyces cerevisiae 850630 NP_013072.1 CDS TPO1 NC_001144.4 84803 86563 D Tpo1p; Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane 84803..86563 Saccharomyces cerevisiae 850631 NP_013073.1 CDS ISA1 NC_001144.4 87402 88154 D Mitochondrial matrix protein involved in biogenesis of the iron-sulfur (Fe/S) cluster of Fe/S proteins, isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; Isa1p 87402..88154 Saccharomyces cerevisiae 850632 NP_013074.1 CDS HSP104 NC_001144.4 88622 91348 D Hsp104p; Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation 88622..91348 Saccharomyces cerevisiae 850633 NP_013075.1 CDS PAU17 NC_001144.4 94746 95120 D Putative protein of unknown function; YLL025W is not an essential gene; Pau17p 94746..95120 Saccharomyces cerevisiae 850635 NP_013076.1 CDS SSA2 NC_001144.4 95565 97484 R Ssa2p; ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall complement(95565..97484) Saccharomyces cerevisiae 850636 NP_013077.1 CDS YLL023C NC_001144.4 97996 98835 R Yll023cp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum complement(97996..98835) Saccharomyces cerevisiae 850637 NP_013078.1 CDS HIF1 NC_001144.4 99043 100200 R Non-essential component of the HAT-B histone acetyltransferase complex (Hat1p-Hat2p-Hif1p), localized to the nucleus; has a role in telomeric silencing; Hif1p complement(99043..100200) Saccharomyces cerevisiae 850638 NP_013079.1 CDS SPA2 NC_001144.4 100946 105346 D Spa2p; Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate 100946..105346 Saccharomyces cerevisiae 850639 NP_013081.1 CDS KNS1 NC_001144.4 105685 107898 R Nonessential putative protein kinase of unknown cellular role; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; Kns1p complement(105685..107898) Saccharomyces cerevisiae 850641 NP_013082.1 CDS COX19 NC_001144.4 108675 108971 R Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; Cox19p complement(108675..108971) Saccharomyces cerevisiae 850642 NP_013083.1 CDS DPS1 NC_001144.4 109901 111574 R Dps1p; Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation complement(109901..111574) Saccharomyces cerevisiae 850643 NP_013086.1 CDS BPT1 NC_001144.4 116431 121110 D Bpt1p; ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p 116431..121110 Saccharomyces cerevisiae 850645 NP_013087.1 CDS YLL014W NC_001144.4 121321 121647 D Yll014wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL014W is not an essential gene 121321..121647 Saccharomyces cerevisiae 850646 NP_013088.1 CDS PUF3 NC_001144.4 122074 124713 R Puf3p; Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins complement(122074..124713) Saccharomyces cerevisiae 850647 NP_013089.1 CDS YEH1 NC_001144.4 125533 127254 D Yeh1p; Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes 125533..127254 Saccharomyces cerevisiae 850648 NP_013090.1 CDS SOF1 NC_001144.4 127522 128991 D Sof1p; Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p 127522..128991 Saccharomyces cerevisiae 850649 NP_013091.1 CDS PSR1 NC_001144.4 129329 130612 R Psr1p; Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p complement(129329..130612) Saccharomyces cerevisiae 850650 NP_013092.1 CDS COX17 NC_001144.4 131204 131413 R Cox17p; Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase complement(131204..131413) Saccharomyces cerevisiae 850651 NP_013093.1 CDS DRS1 NC_001144.4 131728 133986 D Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles; Drs1p 131728..133986 Saccharomyces cerevisiae 850652 NP_013094.1 CDS YLL007C NC_001144.4 134301 136298 R Yll007cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene complement(134301..136298) Saccharomyces cerevisiae 850653 NP_878116.1 CDS YLL006W-A NC_001144.4 136344 136520 D Yll006w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 136344..136520 Saccharomyces cerevisiae 1466403 NP_013095.1 CDS MMM1 NC_001144.4 136585 137865 D Mmm1p; Mitochondrial outer membrane protein, component of the Mdm10-Mdm12-Mmm1 complex involved in import and assembly of outer membrane beta-barrel proteins 136585..137865 Saccharomyces cerevisiae 850654 NP_013096.1 CDS SPO75 NC_001144.4 137939 140545 R Spo75p; Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis complement(137939..140545) Saccharomyces cerevisiae 850655 NP_013097.1 CDS ORC3 NC_001144.4 141072 142922 D Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; Orc3p 141072..142922 Saccharomyces cerevisiae 850656 NP_013098.1 CDS SFI1 NC_001144.4 143200 146040 D Sfi1p; Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C 143200..146040 Saccharomyces cerevisiae 850657 NP_013099.1 CDS RTT109 NC_001144.4 146290 147600 D Rtt109p; Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase, acetylates H3-K56; plays a role in regulation of Ty1 transposition 146290..147600 Saccharomyces cerevisiae 850658 NP_013100.1 CDS DNM1 NC_001144.4 147889 150162 D Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis; Dnm1p 147889..150162 Saccharomyces cerevisiae 850686 NP_013101.1 CDS YLR001C NC_001144.4 151388 153976 R Ylr001cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR001C is not an essential gene complement(151388..153976) Saccharomyces cerevisiae 850687 NP_013102.1 CDS NOC3 NC_001144.4 154342 156333 R Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation; Noc3p complement(154342..156333) Saccharomyces cerevisiae 850688 NP_013103.1 CDS YLR003C NC_001144.4 156854 157729 R Ylr003cp; Putative protein of unknown function that may participate in DNA replication; green fluorescent protein (GFP)-fusion protein is localized to the nucleus; YLR003C is not an essential gene complement(156854..157729) Saccharomyces cerevisiae 850689 NP_013104.1 CDS THI73 NC_001144.4 157933 159504 R Thi73p; Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs complement(157933..159504) Saccharomyces cerevisiae 850690 NP_013105.1 CDS SSL1 NC_001144.4 160048 161433 D Ssl1p; Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p 160048..161433 Saccharomyces cerevisiae 850691 NP_013106.1 CDS SSK1 NC_001144.4 161754 163892 R Ssk1p; Cytoplasmic response regulator, part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate complement(161754..163892) Saccharomyces cerevisiae 850692 NP_013107.1 CDS NSE1 NC_001144.4 164391 165401 D Nse1p; Essential subunit of the Mms21-Smc5-Smc6 complex; nuclear protein required for DNA repair and growth 164391..165401 Saccharomyces cerevisiae 850693 NP_013108.1 CDS PAM18 NC_001144.4 165576 166082 R Pam18p; J-protein co-chaperone of the mitochondrial import motor associated with the presequence translocase, with Ssc1p, Tim44p, Mge1p, and Pam16p; stimulates ATPase activity of Ssc1p to drive mitochondrial import; activity is inhibited by Pam16p complement(165576..166082) Saccharomyces cerevisiae 850694 NP_013109.1 CDS RLP24 NC_001144.4 166536 167135 D Rlp24p; Essential protein with similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S ribosomal subunits and required for ribosomal large subunit biogenesis 166536..167135 Saccharomyces cerevisiae 850695 NP_013110.1 CDS TEN1 NC_001144.4 167319 167801 R Ten1p; Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p complement(167319..167801) Saccharomyces cerevisiae 850696 NP_013111.1 CDS LOT6 NC_001144.4 169102 169677 D Lot6p; FMN-dependent NAD(P)H:quinone reductase that may be involved in quinone detoxification; gene expression increases in cultures shifted to a lower temperature 169102..169677 Saccharomyces cerevisiae 850698 NP_013112.2 CDS YLR012C NC_001144.4 169981 170280 R Ylr012cp; Putative protein of unknown function; YLR012C is not an essential gene complement(169981..170280) Saccharomyces cerevisiae 850699 NP_013113.1 CDS GAT3 NC_001144.4 171338 171763 D Protein containing GATA family zinc finger motifs; Gat3p 171338..171763 Saccharomyces cerevisiae 850700 NP_013114.1 CDS PPR1 NC_001144.4 172267 174981 R Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p; Ppr1p complement(172267..174981) Saccharomyces cerevisiae 850701 NP_013115.1 CDS BRE2 NC_001144.4 175226 176743 D Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L; Bre2p 175226..176743 Saccharomyces cerevisiae 850702 NP_013116.1 CDS PML1 NC_001144.4 176801 177415 R Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p; Pml1p complement(176801..177415) Saccharomyces cerevisiae 850703 NP_013117.1 CDS MEU1 NC_001144.4 177607 178620 D Meu1p; Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression 177607..178620 Saccharomyces cerevisiae 850704 NP_013118.1 CDS POM34 NC_001144.4 178706 179605 R Pom34p; Integral membrane protein of the nuclear pore; has an important role in maintaining the architecture of the pore complex complement(178706..179605) Saccharomyces cerevisiae 850705 NP_013119.1 CDS PSR2 NC_001144.4 180287 181480 D Psr2p; Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p 180287..181480 Saccharomyces cerevisiae 850706 NP_013120.1 CDS YEH2 NC_001144.4 181788 183404 R Yeh2p; Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism complement(181788..183404) Saccharomyces cerevisiae 850707 NP_013121.1 CDS IRC25 NC_001144.4 183622 184161 D Irc25p; Chaperone component involved in 20S proteasome assembly; forms a heterodimer with Poc4p; IRC25 is a non-essential gene; null mutant displays increased levels of spontaneous Rad52 foci 183622..184161 Saccharomyces cerevisiae 850708 NP_013122.1 CDS SDO1 NC_001144.4 184173 184925 R Sdo1p; Essential protein involved in 60S ribosome maturation; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archae and eukaryotes complement(184173..184925) Saccharomyces cerevisiae 850709 NP_013123.1 CDS IZH3 NC_001144.4 185497 187128 R Membrane protein involved in zinc metabolism, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity; Izh3p complement(185497..187128) Saccharomyces cerevisiae 850710 NP_013124.1 CDS UBR2 NC_001144.4 187664 193282 R Ubr2p; Cytoplasmic ubiquitin-protein ligase (E3) complement(187664..193282) Saccharomyces cerevisiae 850711 NP_013125.1 CDS SNF7 NC_001144.4 194453 195175 D Snf7p; One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes 194453..195175 Saccharomyces cerevisiae 850712 NP_013126.1 CDS SED5 NC_001144.4 195451 196473 R cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins; Sed5p complement(195451..196473) Saccharomyces cerevisiae 850713 NP_013127.2 CDS AAT2 NC_001144.4 196830 198086 R Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells; Aat2p complement(196830..198086) Saccharomyces cerevisiae 850714 NP_013128.1 CDS ADE16 NC_001144.4 199541 201316 R Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine; Ade16p complement(199541..201316) Saccharomyces cerevisiae 850715 NP_013129.1 CDS RPL15A NC_001144.4 201977 202591 R Rpl15ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA complement(201977..202591) Saccharomyces cerevisiae 850716 NP_013130.1 CDS YLR030W NC_001144.4 203291 204082 D Ylr030wp; Putative protein of unknown function 203291..204082 Saccharomyces cerevisiae 850717 NP_013131.1 CDS YLR031W NC_001144.4 204226 204786 D Ylr031wp; Putative protein of unknown function 204226..204786 Saccharomyces cerevisiae 850718 NP_013132.1 CDS RAD5 NC_001144.4 204992 208501 D Rad5p; DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; contains RING finger domain 204992..208501 Saccharomyces cerevisiae 850719 NP_013133.1 CDS RSC58 NC_001144.4 208762 210270 D Rsc58p; Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance58KDa subunit 208762..210270 Saccharomyces cerevisiae 850720 NP_013134.1 CDS SMF3 NC_001144.4 210513 211934 R Smf3p; Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins complement(210513..211934) Saccharomyces cerevisiae 850721 NP_013135.1 CDS MLH2 NC_001144.4 212370 214457 R Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p; Mlh2p complement(212370..214457) Saccharomyces cerevisiae 850722 NP_013136.1 CDS YLR035C-A NC_001144.4 215441 218908 R Ylr035c-ap; Retrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); YLR035C-A is part of a mutant retrotransposon complement(215441..218908) Saccharomyces cerevisiae 850724 NP_013137.1 CDS YLR036C NC_001144.4 221522 222133 R Ylr036cp; Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein complement(221522..222133) Saccharomyces cerevisiae 850725 NP_013138.1 CDS DAN2 NC_001144.4 222686 223060 R Dan2p; Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth complement(222686..223060) Saccharomyces cerevisiae 850726 NP_013139.1 CDS COX12 NC_001144.4 224922 225173 R Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; Cox12p complement(224922..225173) Saccharomyces cerevisiae 850727 NP_013140.1 CDS RIC1 NC_001144.4 225427 228597 R Ric1p; Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes complement(225427..228597) Saccharomyces cerevisiae 850728 NP_013141.1 CDS YLR040C NC_001144.4 228909 229583 R Ylr040cp; Putative protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; YLR040C is not essential complement(228909..229583) Saccharomyces cerevisiae 850730 NP_013143.1 CDS YLR042C NC_001144.4 229967 230452 R Ylr042cp; Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene complement(229967..230452) Saccharomyces cerevisiae 850731 NP_013144.1 CDS TRX1 NC_001144.4 231703 232014 R Trx1p; Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance complement(231703..232014) Saccharomyces cerevisiae 850732 NP_013145.1 CDS PDC1 NC_001144.4 232391 234082 R Pdc1p; Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism complement(232391..234082) Saccharomyces cerevisiae 850733 NP_013146.1 CDS STU2 NC_001144.4 235038 237704 R Stu2p; Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p complement(235038..237704) Saccharomyces cerevisiae 850734 NP_013147.1 CDS RLA1 NC_001144.4 238002 238814 R Rla1p; Putative membrane protein, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance complement(238002..238814) Saccharomyces cerevisiae 850735 NP_013148.1 CDS FRE8 NC_001144.4 239348 241408 R Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p; Fre8p complement(239348..241408) Saccharomyces cerevisiae 850736 NP_013149.1 CDS RPS0B NC_001144.4 242233 243350 D Rps0bp; Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal join(242233..242322,242682..243350) Saccharomyces cerevisiae 850737 NP_013150.1 CDS YLR049C NC_001144.4 243887 245173 R Ylr049cp; Putative protein of unknown function complement(243887..245173) Saccharomyces cerevisiae 850738 NP_013151.1 CDS YLR050C NC_001144.4 245588 246073 R Ylr050cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene complement(245588..246073) Saccharomyces cerevisiae 850739 NP_013152.1 CDS FCF2 NC_001144.4 246325 246978 R Fcf2p; Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon complement(246325..246978) Saccharomyces cerevisiae 850740 NP_013153.1 CDS IES3 NC_001144.4 247202 247954 D Subunit of the INO80 chromatin remodeling complex; Ies3p 247202..247954 Saccharomyces cerevisiae 850741 NP_013154.1 CDS YLR053C NC_001144.4 248101 248427 R Ylr053cp; Putative protein of unknown function complement(248101..248427) Saccharomyces cerevisiae 850742 NP_013155.2 CDS OSW2 NC_001144.4 248718 250979 R Protein of unknown function proposed to be involved in the assembly of the spore wall; Osw2p complement(join(248718..250861,250949..250979)) Saccharomyces cerevisiae 850743 NP_013156.1 CDS SPT8 NC_001144.4 251273 253081 R Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters; Spt8p complement(251273..253081) Saccharomyces cerevisiae 850744 NP_013157.1 CDS ERG3 NC_001144.4 253862 254959 D Erg3p; C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources 253862..254959 Saccharomyces cerevisiae 850745 NP_013158.1 CDS YLR057W NC_001144.4 255307 257856 D Ylr057wp; Putative protein of unknown function; YLR050C is not an essential gene 255307..257856 Saccharomyces cerevisiae 850746 NP_013159.1 CDS SHM2 NC_001144.4 257993 259402 R Shm2p; Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis complement(257993..259402) Saccharomyces cerevisiae 850747 NP_013160.1 CDS REX2 NC_001144.4 259739 260548 R Rex2p; RNA exonuclease, required for U4 snRNA maturation; functions redundantly with Rnh70p in 5.8S rRNA maturation, and with Rnh70p and Rex3p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases complement(259739..260548) Saccharomyces cerevisiae 850748 NP_013161.1 CDS FRS1 NC_001144.4 260980 262767 D Frs1p; Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; sequence is evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase (Msf1p), but substrate binding is similar 260980..262767 Saccharomyces cerevisiae 850749 NP_013162.1 CDS RPL22A NC_001144.4 263195 263949 D Rpl22ap; Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein join(263195..263206,263596..263949) Saccharomyces cerevisiae 850750 NP_013164.1 CDS YLR063W NC_001144.4 264158 265255 D Ylr063wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene 264158..265255 Saccharomyces cerevisiae 850752 NP_013165.1 CDS YLR064W NC_001144.4 265457 266278 D Ylr064wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR064W is not an essential gene 265457..266278 Saccharomyces cerevisiae 850753 NP_013166.1 CDS YLR065C NC_001144.4 266374 266919 R Ylr065cp; Putative protein of unknown function; YLR065C is not an essential gene complement(266374..266919) Saccharomyces cerevisiae 850754 NP_013167.1 CDS SPC3 NC_001144.4 267170 267724 D Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; Spc3p 267170..267724 Saccharomyces cerevisiae 850755 NP_013168.1 CDS PET309 NC_001144.4 267814 270711 R Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane; Pet309p complement(267814..270711) Saccharomyces cerevisiae 850756 NP_013169.1 CDS FYV7 NC_001144.4 271009 271464 D Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in processing the 35S rRNA primary transcript to generate the 20S and 27SA2 pre-rRNA transcripts; Fyv7p 271009..271464 Saccharomyces cerevisiae 850757 NP_013170.1 CDS MEF1 NC_001144.4 271631 273916 R Mitochondrial elongation factor involved in translational elongation; Mef1p complement(271631..273916) Saccharomyces cerevisiae 850758 NP_013171.1 CDS XYL2 NC_001144.4 274142 275212 R Xylitol dehydrogenase, converts xylitol to D-xylulose in the endogenous xylose utilization pathway; Xyl2p complement(274142..275212) Saccharomyces cerevisiae 850759 NP_013172.1 CDS RGR1 NC_001144.4 275379 278627 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation; Rgr1p complement(275379..278627) Saccharomyces cerevisiae 850760 NP_013173.1 CDS YLR072W NC_001144.4 278863 280944 D Ylr072wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene 278863..280944 Saccharomyces cerevisiae 850761 NP_013174.1 CDS YLR073C NC_001144.4 281020 281622 R Ylr073cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; YLR073C is not an esssential gene complement(281020..281622) Saccharomyces cerevisiae 850762 NP_013175.1 CDS BUD20 NC_001144.4 281957 282457 R Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud20p complement(281957..282457) Saccharomyces cerevisiae 850763 NP_013176.1 CDS RPL10 NC_001144.4 282928 283593 D Rpl10p; Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family 282928..283593 Saccharomyces cerevisiae 850764 NP_013178.1 CDS FMP25 NC_001144.4 283873 285624 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp25p 283873..285624 Saccharomyces cerevisiae 850766 NP_013179.1 CDS BOS1 NC_001144.4 285737 286560 R v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi; Bos1p complement(join(285737..286468,286558..286560)) Saccharomyces cerevisiae 850767 NP_013180.1 CDS SIC1 NC_001144.4 286821 287675 D Sic1p; Inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 286821..287675 Saccharomyces cerevisiae 850768 NP_013181.1 CDS EMP46 NC_001144.4 287917 289251 D Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport; Emp46p 287917..289251 Saccharomyces cerevisiae 850769 NP_013182.1 CDS GAL2 NC_001144.4 290213 291937 D Galactose permease, required for utilization of galactose; also able to transport glucose; Gal2p 290213..291937 Saccharomyces cerevisiae 850770 NP_013183.1 CDS SRL2 NC_001144.4 292395 293573 R Srl2p; Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation complement(292395..293573) Saccharomyces cerevisiae 850771 NP_013184.1 CDS EMP70 NC_001144.4 294092 296095 R Protein whose 24kDa cleavage product is found in endosome-enriched membrane fractions, predicted to be a transmembrane protein; Emp70p complement(294092..296095) Saccharomyces cerevisiae 850772 NP_013185.1 CDS RAX2 NC_001144.4 296590 300252 R Rax2p; N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p complement(296590..300252) Saccharomyces cerevisiae 850773 NP_013186.1 CDS ARP6 NC_001144.4 300674 301990 R Arp6p; Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A complement(300674..301990) Saccharomyces cerevisiae 850774 NP_013187.1 CDS SMC4 NC_001144.4 302244 306500 D Subunit of the condensin complex, which reorganizes chromosomes during cell division, forms a stable complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity and promotes knotting of circular DNA; potential Cdc28p substrate; Smc4p 302244..306500 Saccharomyces cerevisiae 850775 NP_013188.1 CDS CSF1 NC_001144.4 306856 315732 R Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Csf1p complement(306856..315732) Saccharomyces cerevisiae 850776 NP_013189.1 CDS GAA1 NC_001144.4 316108 317952 D Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER; Gaa1p 316108..317952 Saccharomyces cerevisiae 850777 NP_013190.1 CDS ALT1 NC_001144.4 318238 320016 R Putative alanine transaminase (glutamic pyruvic transaminase); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Alt1p complement(318238..320016) Saccharomyces cerevisiae 850778 NP_013191.1 CDS XDJ1 NC_001144.4 320702 322081 D Xdj1p; Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 320702..322081 Saccharomyces cerevisiae 850779 NP_013192.1 CDS YLR091W NC_001144.4 322298 323179 D Ylr091wp; Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR091W is not an esssential gene 322298..323179 Saccharomyces cerevisiae 850780 NP_013193.1 CDS SUL2 NC_001144.4 323545 326226 D Sul2p; High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates 323545..326226 Saccharomyces cerevisiae 850781 NP_013194.2 CDS NYV1 NC_001144.4 326514 327416 R v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane; Nyv1p complement(join(326514..327259,327401..327416)) Saccharomyces cerevisiae 850782 NP_013195.1 CDS GIS3 NC_001144.4 327731 329239 R Gis3p complement(327731..329239) Saccharomyces cerevisiae 850783 NP_013196.1 CDS IOC2 NC_001144.4 329678 332116 R Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif; Ioc2p complement(329678..332116) Saccharomyces cerevisiae 850784 NP_013197.1 CDS KIN2 NC_001144.4 332591 336034 D Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p; Kin2p 332591..336034 Saccharomyces cerevisiae 850785 NP_013198.1 CDS HRT3 NC_001144.4 336232 337266 R Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies; Hrt3p complement(336232..337266) Saccharomyces cerevisiae 850786 NP_013199.1 CDS CHA4 NC_001144.4 337528 339474 R DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain; Cha4p complement(337528..339474) Saccharomyces cerevisiae 850787 NP_013200.1 CDS ICT1 NC_001144.4 339745 340929 R Protein of unknown function, null mutation leads to an increase in sensitivity to Calcofluor white; expression of the gene is induced in the presence of isooctane; Ict1p complement(339745..340929) Saccharomyces cerevisiae 850788 NP_076901.1 CDS YLR099W-A NC_001144.4 341326 341589 D Ylr099w-ap; Putative protein of unknown function 341326..341589 Saccharomyces cerevisiae 850789 NP_013201.1 CDS ERG27 NC_001144.4 341811 342854 D Erg27p; 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs 341811..342854 Saccharomyces cerevisiae 850790 NP_013203.1 CDS APC9 NC_001144.4 342971 343768 R Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Apc9p complement(342971..343768) Saccharomyces cerevisiae 850792 NP_013204.1 CDS CDC45 NC_001144.4 343990 345942 R DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes; Cdc45p complement(343990..345942) Saccharomyces cerevisiae 850793 NP_013205.1 CDS YLR104W NC_001144.4 346586 346981 D Ylr104wp; Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate 346586..346981 Saccharomyces cerevisiae 850794 NP_013206.1 CDS SEN2 NC_001144.4 347048 348181 R Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease; Sen2p complement(347048..348181) Saccharomyces cerevisiae 850795 NP_013207.1 CDS MDN1 NC_001144.4 349007 363739 R Mdn1p; Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm complement(349007..363739) Saccharomyces cerevisiae 850796 NP_013208.1 CDS REX3 NC_001144.4 364117 365331 D Rex3p; RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases 364117..365331 Saccharomyces cerevisiae 850797 NP_013209.1 CDS YLR108C NC_001144.4 366668 368125 R Ylr108cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene complement(366668..368125) Saccharomyces cerevisiae 850798 NP_013210.1 CDS AHP1 NC_001144.4 368782 369312 D Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p; Ahp1p 368782..369312 Saccharomyces cerevisiae 850799 NP_013211.1 CDS CCW12 NC_001144.4 369698 370099 R Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor; Ccw12p complement(369698..370099) Saccharomyces cerevisiae 850800 NP_013214.1 CDS HOG1 NC_001144.4 371621 372928 D Mitogen-activated protein kinase involved in osmoregulation via three independent osmosensors; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; Hog1p 371621..372928 Saccharomyces cerevisiae 850803 NP_013215.1 CDS AVL9 NC_001144.4 374945 377239 R Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; Avl9p complement(374945..377239) Saccharomyces cerevisiae 850805 NP_013216.1 CDS CFT2 NC_001144.4 377986 380565 D Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.; Cft2p 377986..380565 Saccharomyces cerevisiae 850806 NP_013217.1 CDS MSL5 NC_001144.4 380823 382253 D Msl5p; Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA 380823..382253 Saccharomyces cerevisiae 850807 NP_013218.1 CDS CLF1 NC_001144.4 382472 384535 R Essential splicesome assembly factor; contains multiple tetratricopeptide repeat (TPR) protein-binding motifs and interacts specifically with many spliceosome components, may serve as a scaffold during splicesome assembly; Clf1p complement(382472..384535) Saccharomyces cerevisiae 850808 NP_013219.1 CDS YLR118C NC_001144.4 384726 385409 R Ylr118cp; Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS complement(384726..385409) Saccharomyces cerevisiae 850809 NP_013220.1 CDS SRN2 NC_001144.4 385535 386176 D Srn2p; Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus 385535..386176 Saccharomyces cerevisiae 850810 NP_013221.1 CDS YPS1 NC_001144.4 386512 388221 R Yps1p; Aspartic protease, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor complement(386512..388221) Saccharomyces cerevisiae 850811 NP_013222.1 CDS YPS3 NC_001144.4 388745 390271 R Yps3p; Aspartic protease, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor complement(388745..390271) Saccharomyces cerevisiae 850812 NP_013226.1 CDS YLR125W NC_001144.4 393485 393895 D Ylr125wp; Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene 393485..393895 Saccharomyces cerevisiae 850816 NP_013227.1 CDS YLR126C NC_001144.4 394766 395521 R Ylr126cp; Putative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein complement(394766..395521) Saccharomyces cerevisiae 850817 NP_013228.1 CDS APC2 NC_001144.4 395759 398320 R Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p; Apc2p complement(395759..398320) Saccharomyces cerevisiae 850818 NP_013229.1 CDS DCN1 NC_001144.4 398531 399434 D Putative Nedd8 ligase; binds Nedd8; involved in cullin neddylation; not essential; similar to C.elegans DCN-1; contains UBA-like ubiquitin-binding domain and a DUF298 domain; Dcn1p join(398531..398533,398628..399434) Saccharomyces cerevisiae 850819 NP_013230.1 CDS DIP2 NC_001144.4 399658 402489 D Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex; Dip2p 399658..402489 Saccharomyces cerevisiae 850820 NP_013231.1 CDS ZRT2 NC_001144.4 402795 404063 R Zrt2p; Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor complement(402795..404063) Saccharomyces cerevisiae 850821 NP_013232.1 CDS ACE2 NC_001144.4 404511 406823 R Transcription factor that activates expression of early G1-specific genes, localizes to daughter cell nuclei after cytokinesis and delays G1 progression in daughters, localization is regulated by phosphorylation; potential Cdc28p substrate; Ace2p complement(404511..406823) Saccharomyces cerevisiae 850822 NP_013233.1 CDS YLR132C NC_001144.4 407284 408156 R Ylr132cp; Protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene complement(407284..408156) Saccharomyces cerevisiae 850823 NP_013234.1 CDS CKI1 NC_001144.4 408446 410194 D Cki1p; Choline kinase, catalyzing the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway 408446..410194 Saccharomyces cerevisiae 850824 NP_013235.1 CDS PDC5 NC_001144.4 410724 412415 D Pdc5p; Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism 410724..412415 Saccharomyces cerevisiae 850825 NP_013236.1 CDS SLX4 NC_001144.4 413282 415528 D Subunit of a complex, with Slx1p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p; Slx4p 413282..415528 Saccharomyces cerevisiae 850826 NP_013237.1 CDS TIS11 NC_001144.4 415802 416659 R mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; Tis11p complement(415802..416659) Saccharomyces cerevisiae 850827 NP_013238.1 CDS YLR137W NC_001144.4 417007 418110 D Ylr137wp; Putative protein of unknown function 417007..418110 Saccharomyces cerevisiae 850828 NP_013239.1 CDS NHA1 NC_001144.4 418438 421395 D Nha1p; Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH 418438..421395 Saccharomyces cerevisiae 850829 NP_013240.1 CDS SLS1 NC_001144.4 421543 423474 R Sls1p; Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery complement(421543..423474) Saccharomyces cerevisiae 850830 NP_013242.1 CDS RRN5 NC_001144.4 423684 424775 D Rrn5p; Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p 423684..424775 Saccharomyces cerevisiae 850832 NP_013243.1 CDS PUT1 NC_001144.4 425187 426617 D Put1p; Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source 425187..426617 Saccharomyces cerevisiae 850833 NP_013244.1 CDS YLR143W NC_001144.4 427330 429387 D Ylr143wp; Putative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene 427330..429387 Saccharomyces cerevisiae 850835 NP_013245.1 CDS ACF2 NC_001144.4 429678 432017 R Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; Acf2p complement(429678..432017) Saccharomyces cerevisiae 850836 NP_013246.1 CDS RMP1 NC_001144.4 432169 432774 D Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P; Rmp1p 432169..432774 Saccharomyces cerevisiae 850837 NP_013247.1 CDS SPE4 NC_001144.4 432824 433726 R Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid; Spe4p complement(432824..433726) Saccharomyces cerevisiae 850838 NP_001032582.1 CDS YLR146W-A NC_001144.4 433871 434059 D Ylr146w-ap; Putative protein of unknown function 433871..434059 Saccharomyces cerevisiae 3799977 NP_013248.1 CDS SMD3 NC_001144.4 434159 434464 R Smd3p; Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 complement(434159..434464) Saccharomyces cerevisiae 850839 NP_013249.1 CDS PEP3 NC_001144.4 434642 437398 D Pep3p; Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis 434642..437398 Saccharomyces cerevisiae 850840 NP_013250.1 CDS YLR149C NC_001144.4 437632 439824 R Ylr149cp; Putative protein of unknown function; YLR149C is not an essential gene complement(437632..439824) Saccharomyces cerevisiae 850841 NP_013251.1 CDS STM1 NC_001144.4 440468 441289 D Stm1p; Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations 440468..441289 Saccharomyces cerevisiae 850843 NP_013252.1 CDS PCD1 NC_001144.4 441716 442738 R Peroxisomal nudix pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; Pcd1p complement(441716..442738) Saccharomyces cerevisiae 850844 NP_013253.1 CDS YLR152C NC_001144.4 442959 444689 R Ylr152cp; Putative protein of unknown function; YLR152C is not an essential gene complement(442959..444689) Saccharomyces cerevisiae 850845 NP_013254.1 CDS ACS2 NC_001144.4 445525 447576 R Acs2p; Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions complement(445525..447576) Saccharomyces cerevisiae 850846 NP_013255.1 CDS RNH203 NC_001144.4 447983 448315 R Rnh203p; Ribonuclease H2 subunit, required for RNase H2 activity complement(447983..448315) Saccharomyces cerevisiae 850847 NP_690845.1 CDS TAR1 NC_001144.4 454697 455071 D Tar1p; Mitochondrial protein of unknown function, overexpression suppresses an rpo41 mutation affecting mitochondrial RNA polymerase; encoded within the 25S rRNA gene on the opposite strand 454697..455071 Saccharomyces cerevisiae 850849 NP_878121.1 CDS YLR154C-G NC_001144.4 462523 462672 R Ylr154c-gp; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 complement(462523..462672) Saccharomyces cerevisiae 1466409 NP_878122.1 CDS YLR154C-H NC_001144.4 468828 468959 R Ylr154c-hp; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-2 complement(468828..468959) Saccharomyces cerevisiae 1466410 NP_013256.1 CDS ASP3-1 NC_001144.4 469318 470406 R Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C; Asp3-1p complement(469318..470406) Saccharomyces cerevisiae 850850 NP_757337.1 CDS YLR156W NC_001144.4 472114 472458 D Ylr156wp; Putative protein of unknown function; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis 472114..472458 Saccharomyces cerevisiae 850851 NP_878123.1 CDS YLR156C-A NC_001144.4 472480 472611 R Ylr156c-ap; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-3 complement(472480..472611) Saccharomyces cerevisiae 1466411 NP_013258.1 CDS ASP3-2 NC_001144.4 472970 474058 R Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C; Asp3-2p complement(472970..474058) Saccharomyces cerevisiae 850852 NP_878124.1 CDS YLR157W-D NC_001144.4 475765 475977 D Ylr157w-dp; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 475765..475977 Saccharomyces cerevisiae 1466412 NP_058169.1 CDS YLR157C-B NC_001144.4 476334 481602 R Ylr157c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(476334..480296,480298..481602)) Saccharomyces cerevisiae 850853 NP_058170.1 CDS YLR157C-A NC_001144.4 480280 481602 R Ylr157c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(480280..481602) Saccharomyces cerevisiae 850854 NP_878125.1 CDS YLR157W-E NC_001144.4 481874 482038 D Ylr157w-ep; Putative protein of unknown function identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element 481874..482038 Saccharomyces cerevisiae 1466413 NP_878126.1 CDS YLR157C-C NC_001144.4 482060 482191 R Ylr157c-cp; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-4 complement(482060..482191) Saccharomyces cerevisiae 1466414 NP_013259.1 CDS ASP3-3 NC_001144.4 482550 483638 R Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C; Asp3-3p complement(482550..483638) Saccharomyces cerevisiae 850855 NP_757338.1 CDS YLR159W NC_001144.4 485346 485690 D Ylr159wp; Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 485346..485690 Saccharomyces cerevisiae 850856 NP_878127.1 CDS YLR159C-A NC_001144.4 485712 485843 R Ylr159c-ap; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-5 complement(485712..485843) Saccharomyces cerevisiae 1466415 NP_013261.1 CDS ASP3-4 NC_001144.4 486202 487290 R Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C; Asp3-4p complement(486202..487290) Saccharomyces cerevisiae 850857 NP_757339.1 CDS YLR161W NC_001144.4 488998 489342 D Ylr161wp; Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 488998..489342 Saccharomyces cerevisiae 850858 NP_013263.1 CDS YLR162W NC_001144.4 489574 489930 D Ylr162wp; Putative protein of unknown function; overexpression confers resistance to the antimicrobial peptide MiAMP1 489574..489930 Saccharomyces cerevisiae 850859 NP_878128.1 CDS YLR162W-A NC_001144.4 490407 490595 D Ylr162w-ap; Putative protein of unknown function identified by fungal homology comparisons and RT-PCR 490407..490595 Saccharomyces cerevisiae 1466416 NP_013264.1 CDS MAS1 NC_001144.4 491868 493256 R Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins; Mas1p complement(491868..493256) Saccharomyces cerevisiae 850860 NP_013265.1 CDS YLR164W NC_001144.4 493885 494391 D Ylr164wp; Mitochondrial inner membrane of unknown function; similar to Tim18p and Sdh4p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner 493885..494391 Saccharomyces cerevisiae 850861 NP_013266.1 CDS PUS5 NC_001144.4 494496 495260 R Pus5p; Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability complement(494496..495260) Saccharomyces cerevisiae 850862 NP_013267.1 CDS SEC10 NC_001144.4 495431 498046 R Sec10p; Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis complement(495431..498046) Saccharomyces cerevisiae 850863 NP_013268.1 CDS RPS31 NC_001144.4 498949 499407 D Rps31p; Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B 498949..499407 Saccharomyces cerevisiae 850864 NP_013269.1 CDS AIM30 NC_001144.4 499580 500272 R Putative protein of unknown function that may be involved in intramitochondrial sorting; similar to Ups1p and to human PRELI; GFP-tagged protein localizes to mitochondria; required for wild-type respiratory growth; Aim30p complement(499580..500272) Saccharomyces cerevisiae 850865 NP_013271.1 CDS APS1 NC_001144.4 500581 501051 R Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex; Aps1p complement(500581..501051) Saccharomyces cerevisiae 850868 NP_013273.1 CDS DPH5 NC_001144.4 501262 502164 R Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm; Dph5p complement(501262..502164) Saccharomyces cerevisiae 850869 NP_013274.1 CDS YLR173W NC_001144.4 502423 504249 D Ylr173wp; Putative protein of unknown function 502423..504249 Saccharomyces cerevisiae 850870 NP_013275.1 CDS IDP2 NC_001144.4 504593 505831 D Idp2p; Cytosolic NADP-specific isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose 504593..505831 Saccharomyces cerevisiae 850871 NP_013276.1 CDS CBF5 NC_001144.4 506136 507587 D Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; Cbf5p 506136..507587 Saccharomyces cerevisiae 850872 NP_013277.1 CDS RFX1 NC_001144.4 507799 510234 R Rfx1p; Protein involved in DNA damage and replication checkpoint pathway; recruits repressors Tup1p and Cyc8p to promoters of DNA damage-inducible genes; similar to a family of mammalian DNA binding RFX1-4 proteins complement(507799..510234) Saccharomyces cerevisiae 850873 NP_013278.1 CDS YLR177W NC_001144.4 511056 512942 D Ylr177wp; Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; YLR177W is not an essential gene 511056..512942 Saccharomyces cerevisiae 850874 NP_013279.1 CDS TFS1 NC_001144.4 513164 513823 R Tfs1p; Carboxypeptidase Y inhibitor, function requires acetylation by the NatB N-terminal acetyltransferase; phosphatidylethanolamine-binding protein involved in protein kinase A signaling pathway complement(513164..513823) Saccharomyces cerevisiae 850875 NP_013280.1 CDS YLR179C NC_001144.4 514110 514715 R Ylr179cp; Protein of unknown function, transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus; YLR179C is not essential complement(514110..514715) Saccharomyces cerevisiae 850876 NP_013281.1 CDS SAM1 NC_001144.4 515264 516412 D Sam1p; S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) 515264..516412 Saccharomyces cerevisiae 850877 NP_013282.1 CDS VTA1 NC_001144.4 516680 517672 R Vta1p; Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligimeriztion; binds to Vps4p, Vps20p, and Vps60p; may act at a late step in MVB formation complement(516680..517672) Saccharomyces cerevisiae 850878 NP_013283.1 CDS SWI6 NC_001144.4 517942 520353 D Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate; Swi6p 517942..520353 Saccharomyces cerevisiae 850879 NP_013284.1 CDS TOS4 NC_001144.4 520545 522014 R Transcription factor that binds to a number of promoter regions, particularly promoters of some genes involved in pheromone response and cell cycle; potential Cdc28p substrate; expression is induced in G1 by bound SBF; Tos4p complement(520545..522014) Saccharomyces cerevisiae 850880 NP_013286.1 CDS RPL37A NC_001144.4 522665 523290 D Rpl37ap; Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Bp and to rat L37 ribosomal protein join(522665..522671,523031..523290) Saccharomyces cerevisiae 850882 NP_013287.1 CDS EMG1 NC_001144.4 523634 524392 D Protein required for the maturation of the 18S rRNA and for 40S ribosome production; associated with spindle/microtubules; nuclear localization depends on physical interaction with Nop14p; may bind snoRNAs; Emg1p 523634..524392 Saccharomyces cerevisiae 850883 NP_013288.1 CDS SKG3 NC_001144.4 524867 527947 D Skg3p; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p 524867..527947 Saccharomyces cerevisiae 850884 NP_013289.1 CDS MDL1 NC_001144.4 528302 530389 D Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress; Mdl1p 528302..530389 Saccharomyces cerevisiae 850885 NP_013290.1 CDS ATG26 NC_001144.4 530799 534395 R UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids, involved in autophagy; Atg26p complement(530799..534395) Saccharomyces cerevisiae 850886 NP_013291.1 CDS MMR1 NC_001144.4 535216 536691 D Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p; Mmr1p 535216..536691 Saccharomyces cerevisiae 850887 NP_013292.1 CDS PEX13 NC_001144.4 537274 538434 D Pex13p; Integral peroxisomal membrane required for the translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p 537274..538434 Saccharomyces cerevisiae 850888 NP_013293.1 CDS HCR1 NC_001144.4 538794 539591 R Hcr1p; Dual function protein involved in translation initiation as a substoichiometric component of eukaryotic translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA complement(538794..539591) Saccharomyces cerevisiae 850889 NP_013294.1 CDS UPS1 NC_001144.4 540011 540538 R Ups1p; Mitochondrial intermembrane space protein that regulates alternative processing and sorting of Mgm1p and other proteins; required for normal mitochondrial morphology; ortholog of human PRELI complement(540011..540538) Saccharomyces cerevisiae 850890 NP_013295.1 CDS YLR194C NC_001144.4 540811 541575 R Ylr194cp; Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress complement(540811..541575) Saccharomyces cerevisiae 850891 NP_013296.1 CDS NMT1 NC_001144.4 541939 543306 R Nmt1p; N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction complement(541939..543306) Saccharomyces cerevisiae 850892 NP_013297.1 CDS PWP1 NC_001144.4 543970 545700 D Pwp1p; Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily 543970..545700 Saccharomyces cerevisiae 850893 NP_013298.1 CDS SIK1 NC_001144.4 546099 547613 D Sik1p; Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects 546099..547613 Saccharomyces cerevisiae 850894 NP_013300.2 CDS PBA1 NC_001144.4 547855 548771 R Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly; Pba1p complement(join(547855..548679,548766..548771)) Saccharomyces cerevisiae 850896 NP_013301.1 CDS YKE2 NC_001144.4 549014 549358 D Yke2p; Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin 549014..549358 Saccharomyces cerevisiae 850897 NP_013302.1 CDS COQ9 NC_001144.4 549513 550295 R Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; Coq9p complement(549513..550295) Saccharomyces cerevisiae 850898 NP_013304.1 CDS MSS51 NC_001144.4 550652 551962 R Nuclear encoded protein required for translation of COX1 mRNA; binds to Cox1 protein; Mss51p complement(550652..551962) Saccharomyces cerevisiae 850900 NP_013305.1 CDS QRI5 NC_001144.4 552272 552607 D Qri5p; Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA 552272..552607 Saccharomyces cerevisiae 850901 NP_013306.2 CDS HMX1 NC_001144.4 552726 553679 R Hmx1p; ER localized, heme-binding peroxidase involved in the degradation of heme; does not exhibit heme oxygenase activity despite similarity to heme oxygenases; expression regulated by AFT1 complement(552726..553679) Saccharomyces cerevisiae 850902 NP_013307.1 CDS ENT2 NC_001144.4 554580 556421 D Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; Ent2p 554580..556421 Saccharomyces cerevisiae 850903 NP_013308.1 CDS HRD3 NC_001144.4 556790 559291 D Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events; Hrd3p 556790..559291 Saccharomyces cerevisiae 850904 NP_013309.1 CDS SEC13 NC_001144.4 559553 560446 D Sec13p; Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles 559553..560446 Saccharomyces cerevisiae 850905 NP_013310.1 CDS PNP1 NC_001144.4 560799 561734 R Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway; Pnp1p complement(560799..561734) Saccharomyces cerevisiae 850906 NP_013311.1 CDS CLB4 NC_001144.4 562010 563392 D Clb4p; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation 562010..563392 Saccharomyces cerevisiae 850907 NP_013312.2 CDS YLR211C NC_001144.4 563794 564533 R Ylr211cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron complement(join(563794..564456,564516..564533)) Saccharomyces cerevisiae 850908 NP_013313.1 CDS TUB4 NC_001144.4 564862 566283 R Tub4p; Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body complement(564862..566283) Saccharomyces cerevisiae 850909 NP_013314.1 CDS CRR1 NC_001144.4 566656 567924 R Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation; Crr1p complement(566656..567924) Saccharomyces cerevisiae 850910 NP_013315.1 CDS FRE1 NC_001144.4 568569 570629 D Fre1p; Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels 568569..570629 Saccharomyces cerevisiae 850911 NP_013316.1 CDS CDC123 NC_001144.4 570778 571860 R Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein; Cdc123p complement(570778..571860) Saccharomyces cerevisiae 850912 NP_013317.1 CDS CPR6 NC_001144.4 572098 573213 R Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; Cpr6p complement(572098..573213) Saccharomyces cerevisiae 850914 NP_013319.1 CDS YLR218C NC_001144.4 573468 573920 R Ylr218cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR218C is not essential; mutants exhibit glycogen storage defects and growth defects on a non-fermentable carbon source complement(573468..573920) Saccharomyces cerevisiae 850915 NP_013320.1 CDS MSC3 NC_001144.4 574153 576339 D Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; Msc3p 574153..576339 Saccharomyces cerevisiae 850916 NP_013321.1 CDS CCC1 NC_001144.4 576827 577795 D Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; Ccc1p 576827..577795 Saccharomyces cerevisiae 850917 NP_013322.1 CDS RSA3 NC_001144.4 578364 579026 R Rsa3p; Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus complement(578364..579026) Saccharomyces cerevisiae 850918 NP_013323.1 CDS UTP13 NC_001144.4 579320 581773 R Utp13p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA complement(579320..581773) Saccharomyces cerevisiae 850919 NP_013324.1 CDS IFH1 NC_001144.4 582235 585492 R Ifh1p; Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate complement(582235..585492) Saccharomyces cerevisiae 850920 NP_013325.1 CDS YLR224W NC_001144.4 586466 587575 D Ylr224wp; F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene 586466..587575 Saccharomyces cerevisiae 850921 NP_013326.1 CDS YLR225C NC_001144.4 587697 588920 R Ylr225cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene complement(587697..588920) Saccharomyces cerevisiae 850922 NP_013327.1 CDS BUR2 NC_001144.4 589356 590543 D Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II; Bur2p 589356..590543 Saccharomyces cerevisiae 850923 NP_013328.1 CDS ADY4 NC_001144.4 590564 592045 R Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; Ady4p complement(590564..592045) Saccharomyces cerevisiae 850924 NP_058171.1 CDS YLR227W-B NC_001144.4 593440 598708 D Ylr227w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(593440..594744,594746..598708) Saccharomyces cerevisiae 850927 NP_058172.1 CDS YLR227W-A NC_001144.4 593440 594762 D Ylr227w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 593440..594762 Saccharomyces cerevisiae 850926 NP_013329.1 CDS ECM22 NC_001144.4 600021 602465 R Sterol regulatory element binding protein, regulates transcription of the sterol biosynthetic genes ERG2 and ERG3; member of the fungus-specific Zn[2]-Cys[6] binuclear cluster family of transcription factors; homologous to Upc2p; Ecm22p complement(600021..602465) Saccharomyces cerevisiae 850928 NP_013330.1 CDS CDC42 NC_001144.4 604214 604789 R Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins; Cdc42p complement(604214..604789) Saccharomyces cerevisiae 850930 NP_013332.1 CDS BNA5 NC_001144.4 605760 607121 R Kynureninase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; Bna5p complement(605760..607121) Saccharomyces cerevisiae 850933 NP_013334.1 CDS EST1 NC_001144.4 607427 609526 R TLC1 RNA-associated factor involved in telomere length regulation as the recruitment subunit of the telomerase holoenzyme, has a possible role in activating Est2p-TLC1-RNA bound to the telomere; Est1p complement(607427..609526) Saccharomyces cerevisiae 850934 NP_013335.1 CDS TOP3 NC_001144.4 609785 611755 D Top3p; DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination 609785..611755 Saccharomyces cerevisiae 850935 NP_013338.1 CDS THI7 NC_001144.4 612369 614165 D Thi7p; Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia 612369..614165 Saccharomyces cerevisiae 850938 NP_013339.1 CDS FAR10 NC_001144.4 614757 616193 D Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; Far10p 614757..616193 Saccharomyces cerevisiae 850939 NP_013340.1 CDS LIP2 NC_001144.4 616334 617320 R Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups; Lip2p complement(616334..617320) Saccharomyces cerevisiae 850940 NP_013341.1 CDS VPS34 NC_001144.4 617535 620162 D Vps34p; Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p 617535..620162 Saccharomyces cerevisiae 850941 NP_013342.1 CDS YLR241W NC_001144.4 620475 622823 D Ylr241wp; Putative protein of unknown function, may be involved in detoxification 620475..622823 Saccharomyces cerevisiae 850942 NP_013343.1 CDS ARV1 NC_001144.4 622920 623885 R Protein required for normal intracellular sterol distribution and for sphingolipid metabolism; similar to Nup120p and C. elegans R05H5.5 protein; Arv1p complement(622920..623885) Saccharomyces cerevisiae 850943 NP_013344.1 CDS YLR243W NC_001144.4 624205 625023 D Ylr243wp; Putative protein of unknown function; YLR243W is an essential gene 624205..625023 Saccharomyces cerevisiae 850944 NP_013345.1 CDS MAP1 NC_001144.4 625170 626333 R Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p; Map1p complement(625170..626333) Saccharomyces cerevisiae 850945 NP_013346.1 CDS CDD1 NC_001144.4 626504 626932 R Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm; Cdd1p complement(626504..626932) Saccharomyces cerevisiae 850946 NP_013347.1 CDS ERF2 NC_001144.4 627120 628199 D Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; Erf2p 627120..628199 Saccharomyces cerevisiae 850947 NP_013348.1 CDS IRC20 NC_001144.4 628686 633356 R Putative helicase; localized to mitochondria and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52 foci; Irc20p complement(628686..633356) Saccharomyces cerevisiae 850949 NP_013349.1 CDS RCK2 NC_001144.4 634254 636086 D Rck2p; Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations 634254..636086 Saccharomyces cerevisiae 850950 NP_013350.1 CDS YEF3 NC_001144.4 636782 639916 D Yef3p; Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP 636782..639916 Saccharomyces cerevisiae 850951 NP_013351.1 CDS SSP120 NC_001144.4 640319 641023 D Ssp120p; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern 640319..641023 Saccharomyces cerevisiae 850952 NP_013352.1 CDS SYM1 NC_001144.4 641466 642059 D Sym1p; Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 641466..642059 Saccharomyces cerevisiae 850953 NP_013354.1 CDS YLR253W NC_001144.4 642629 644338 D Ylr253wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene 642629..644338 Saccharomyces cerevisiae 850955 NP_013355.1 CDS NDL1 NC_001144.4 644406 644975 R Ndl1p; Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends complement(644406..644975) Saccharomyces cerevisiae 850956 NP_013357.1 CDS HAP1 NC_001144.4 646417 650925 D Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus; Hap1p 646417..650925 Saccharomyces cerevisiae 850958 NP_058173.1 CDS YLR256W-A NC_001144.4 651119 652441 D Ylr256w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 651119..652441 Saccharomyces cerevisiae 850959 NP_013358.1 CDS YLR257W NC_001144.4 658828 659793 D Ylr257wp; Putative protein of unknown function 658828..659793 Saccharomyces cerevisiae 850961 NP_013359.1 CDS GSY2 NC_001144.4 660718 662835 D Gsy2p; Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase 660718..662835 Saccharomyces cerevisiae 850962 NP_013360.1 CDS HSP60 NC_001144.4 663286 665004 R Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated; Hsp60p complement(663286..665004) Saccharomyces cerevisiae 850963 NP_013361.1 CDS LCB5 NC_001144.4 665846 667909 D Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; Lcb5p 665846..667909 Saccharomyces cerevisiae 850964 NP_013363.1 CDS YPT6 NC_001144.4 668246 668893 R Ypt6p; GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 complement(668246..668893) Saccharomyces cerevisiae 850966 NP_013364.1 CDS TMA7 NC_001144.4 669470 669664 R Tma7p; Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin complement(669470..669664) Saccharomyces cerevisiae 850967 NP_013365.1 CDS RED1 NC_001144.4 670342 672825 D Red1p; Protein component of the axial elements of the synaptonemal complex, involved in chromosome segregation during the first meiotic division; interacts with Hop1p; required for wild-type levels of Mek1p kinase activity 670342..672825 Saccharomyces cerevisiae 850968 NP_013366.1 CDS RPS28B NC_001144.4 673133 673336 D Rps28bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Ap and has similarity to rat S28 ribosomal protein 673133..673336 Saccharomyces cerevisiae 850969 NP_878129.1 CDS YLR264C-A NC_001144.4 673830 673946 R Ylr264c-ap; Putative protein of unknown function complement(673830..673946) Saccharomyces cerevisiae 1466417 NP_013367.1 CDS NEJ1 NC_001144.4 674429 675457 R Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p; Nej1p complement(674429..675457) Saccharomyces cerevisiae 850970 NP_013368.1 CDS PDR8 NC_001144.4 675621 677726 R Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; Pdr8p complement(675621..677726) Saccharomyces cerevisiae 850971 NP_013369.1 CDS BOP2 NC_001144.4 678214 679926 D Protein of unknown function, overproduction suppresses a pam1 slv3 double null mutation; Bop2p 678214..679926 Saccharomyces cerevisiae 850972 NP_013370.1 CDS SEC22 NC_001144.4 680202 680846 D Sec22p; R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog 680202..680846 Saccharomyces cerevisiae 850973 NP_013372.1 CDS DCS1 NC_001144.4 681188 682240 D Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; Dcs1p 681188..682240 Saccharomyces cerevisiae 850974 NP_013373.1 CDS YLR271W NC_001144.4 682739 683563 D Ylr271wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS 682739..683563 Saccharomyces cerevisiae 850976 NP_013374.1 CDS YCS4 NC_001144.4 683674 687204 R Ycs4p; Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin and silencing at the mating type locus complement(683674..687204) Saccharomyces cerevisiae 850977 NP_013375.1 CDS PIG1 NC_001144.4 689085 691031 R Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase; Pig1p complement(689085..691031) Saccharomyces cerevisiae 850979 NP_013376.1 CDS MCM5 NC_001144.4 691557 693884 D Mcm5p; Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase 691557..693884 Saccharomyces cerevisiae 850980 NP_013377.1 CDS SMD2 NC_001144.4 694380 694802 D Smd2p; Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 join(694380..694384,694475..694802) Saccharomyces cerevisiae 850981 NP_013378.1 CDS DBP9 NC_001144.4 695048 696832 R Dbp9p; ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subunit complement(695048..696832) Saccharomyces cerevisiae 850982 NP_013379.1 CDS YSH1 NC_001144.4 697158 699497 R Ysh1p; Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs complement(697158..699497) Saccharomyces cerevisiae 850983 NP_013380.1 CDS YLR278C NC_001144.4 700001 704026 R Zinc-cluster protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; YLR278C is not an essential gene; Ylr278cp complement(700001..704026) Saccharomyces cerevisiae 850984 NP_013383.1 CDS YLR281C NC_001144.4 704495 704962 R Ylr281cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene complement(704495..704962) Saccharomyces cerevisiae 850987 NP_013385.1 CDS YLR283W NC_001144.4 705188 706132 D Ylr283wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene 705188..706132 Saccharomyces cerevisiae 850988 NP_013386.1 CDS ECI1 NC_001144.4 706200 707042 R Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; Eci1p complement(706200..707042) Saccharomyces cerevisiae 850990 NP_013387.1 CDS NNT1 NC_001144.4 707362 708147 D Putative nicotinamide N-methyltransferase, has a role in rDNA silencing and in lifespan determination; Nnt1p 707362..708147 Saccharomyces cerevisiae 850991 NP_878130.1 CDS YLR285C-A NC_001144.4 708170 708340 R Ylr285c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(708170..708340) Saccharomyces cerevisiae 1466418 NP_013388.1 CDS CTS1 NC_001144.4 708450 710138 R Endochitinase, required for cell separation after mitosis; transcriptional activation during late G and early M cell cycle phases is mediated by transcription factor Ace2p; Cts1p complement(708450..710138) Saccharomyces cerevisiae 850992 NP_013389.1 CDS YLR287C NC_001144.4 710993 712060 R Ylr287cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene complement(710993..712060) Saccharomyces cerevisiae 850993 NP_013390.1 CDS RPS30A NC_001144.4 712539 713160 R Rps30ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Bp and has similarity to rat S30 ribosomal protein complement(join(712539..712727,713158..713160)) Saccharomyces cerevisiae 850994 NP_013391.1 CDS MEC3 NC_001144.4 713482 714906 R Mec3p; DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 complement(713482..714906) Saccharomyces cerevisiae 850995 NP_013392.1 CDS GUF1 NC_001144.4 715091 717028 D Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans; Guf1p 715091..717028 Saccharomyces cerevisiae 850996 NP_013393.1 CDS YLR290C NC_001144.4 717147 717980 R Ylr290cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene complement(717147..717980) Saccharomyces cerevisiae 850997 NP_013394.1 CDS GCD7 NC_001144.4 718319 719464 R Gcd7p; Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression complement(718319..719464) Saccharomyces cerevisiae 850998 NP_013395.1 CDS SEC72 NC_001144.4 719791 720372 R Sec72p; Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER complement(719791..720372) Saccharomyces cerevisiae 850999 NP_013396.1 CDS GSP1 NC_001144.4 720773 721432 R Gsp1p; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Prp20p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; yeast Gsp2p homolog complement(720773..721432) Saccharomyces cerevisiae 851000 NP_013398.1 CDS ATP14 NC_001144.4 722001 722375 R Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; Atp14p complement(722001..722375) Saccharomyces cerevisiae 851002 NP_013400.1 CDS YLR297W NC_001144.4 724046 724435 D Ylr297wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YLR297W is not an essential gene 724046..724435 Saccharomyces cerevisiae 851004 NP_013401.1 CDS YHC1 NC_001144.4 724723 725418 R Yhc1p; Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site complement(724723..725418) Saccharomyces cerevisiae 851005 NP_013402.1 CDS ECM38 NC_001144.4 726071 728053 D Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation; Ecm38p 726071..728053 Saccharomyces cerevisiae 851006 NP_013403.1 CDS EXG1 NC_001144.4 728957 730303 D Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; Exg1p 728957..730303 Saccharomyces cerevisiae 851007 NP_013404.1 CDS YLR301W NC_001144.4 730827 731561 D Ylr301wp; Protein of unknown function that interacts with Sec72p 730827..731561 Saccharomyces cerevisiae 851008 NP_013406.1 CDS MET17 NC_001144.4 732544 733878 D Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis; Met17p 732544..733878 Saccharomyces cerevisiae 851010 NP_013407.1 CDS ACO1 NC_001144.4 735214 737550 R Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy; Aco1p complement(735214..737550) Saccharomyces cerevisiae 851013 NP_013408.1 CDS STT4 NC_001144.4 738163 743865 R Stt4p; Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization complement(738163..743865) Saccharomyces cerevisiae 851014 NP_013409.1 CDS UBC12 NC_001144.4 744153 744853 D Ubc12p; Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes join(744153..744155,744290..744853) Saccharomyces cerevisiae 851015 NP_013410.1 CDS CDA1 NC_001144.4 745622 746527 D Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall; Cda1p 745622..746527 Saccharomyces cerevisiae 851016 NP_878131.1 CDS YLR307C-A NC_001144.4 746850 747113 R Ylr307c-ap; Putative protein of unknown function complement(746850..747113) Saccharomyces cerevisiae 1466419 NP_013411.1 CDS CDA2 NC_001144.4 747939 748877 D Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall; Cda2p 747939..748877 Saccharomyces cerevisiae 851017 NP_013412.1 CDS IMH1 NC_001144.4 749036 751771 R Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi; Imh1p complement(749036..751771) Saccharomyces cerevisiae 851018 NP_013413.1 CDS CDC25 NC_001144.4 752226 756995 R Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; Cdc25p complement(752226..756995) Saccharomyces cerevisiae 851019 NP_013415.1 CDS QNQ1 NC_001144.4 757639 758835 R Qnq1p; Protein of unknown function; null mutant quiescent and non-quiescent cells exhibit reduced reproductive capacity complement(757639..758835) Saccharomyces cerevisiae 851021 NP_013416.2 CDS MRPL15 NC_001144.4 759482 760243 D Mrpl15p; Mitochondrial ribosomal protein of the large subunit 759482..760243 Saccharomyces cerevisiae 851022 NP_013417.2 CDS SPH1 NC_001144.4 760357 762342 R Sph1p; Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p complement(760357..762342) Saccharomyces cerevisiae 851023 NP_013418.1 CDS CDC3 NC_001144.4 762575 764137 R Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM; Cdc3p complement(762575..764137) Saccharomyces cerevisiae 851024 NP_013419.1 CDS NKP2 NC_001144.4 764808 765269 D Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p- Nkp1p-Nkp2p-Ame1p-Mtw1p); Nkp2p 764808..765269 Saccharomyces cerevisiae 851025 NP_013420.2 CDS TAD3 NC_001144.4 765266 766358 R Tad3p; Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs complement(join(765266..766073,766130..766181,766250..766358)) Saccharomyces cerevisiae 851027 NP_013422.1 CDS EST2 NC_001144.4 766542 769196 D Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia; Est2p 766542..769196 Saccharomyces cerevisiae 851028 NP_013423.1 CDS BUD6 NC_001144.4 769318 771684 R Bud6p; Actin- and formin-interacting protein, involved in actin cable nucleation and polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate complement(769318..771684) Saccharomyces cerevisiae 851029 NP_013424.1 CDS MMS22 NC_001144.4 771940 776304 D Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks; Mms22p 771940..776304 Saccharomyces cerevisiae 851030 NP_013425.1 CDS SFH1 NC_001144.4 776584 777864 R Sfh1p; Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progressionand maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog complement(776584..777864) Saccharomyces cerevisiae 851032 NP_013427.1 CDS CWC24 NC_001144.4 778173 778952 R Essential protein, component of a complex containing Cef1p; has similarity to S. pombe Cwf24p; Cwc24p complement(778173..778952) Saccharomyces cerevisiae 851033 NP_013428.1 CDS PEX30 NC_001144.4 779215 780786 D Pex30p; Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p 779215..780786 Saccharomyces cerevisiae 851034 NP_013429.1 CDS RPL38 NC_001144.4 781143 781379 R Rpl38p; Protein component of the large (60S) ribosomal subunit, has similarity to rat L38 ribosomal protein complement(781143..781379) Saccharomyces cerevisiae 851035 NP_013430.1 CDS YLR326W NC_001144.4 782174 782896 D Ylr326wp 782174..782896 Saccharomyces cerevisiae 851036 NP_013431.1 CDS TMA10 NC_001144.4 783127 783387 R Tma10p; Protein of unknown function that associates with ribosomes complement(783127..783387) Saccharomyces cerevisiae 851037 NP_013432.1 CDS NMA1 NC_001144.4 784913 786118 D Nma1p; Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway 784913..786118 Saccharomyces cerevisiae 851039 NP_013433.2 CDS REC102 NC_001144.4 786442 787333 D Rec102p; Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination join(786442..786615,786713..787333) Saccharomyces cerevisiae 851040 NP_013434.1 CDS CHS5 NC_001144.4 787664 789679 D Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; Chs5p 787664..789679 Saccharomyces cerevisiae 851041 NP_013436.1 CDS MID2 NC_001144.4 790676 791806 D O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; Mid2p 790676..791806 Saccharomyces cerevisiae 851042 NP_013437.1 CDS RPS25B NC_001144.4 795573 795899 R Rps25bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein complement(795573..795899) Saccharomyces cerevisiae 851045 NP_013439.1 CDS NUP2 NC_001144.4 797430 799592 D Nup2p; Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization 797430..799592 Saccharomyces cerevisiae 851048 NP_013440.1 CDS SGD1 NC_001144.4 799697 802396 R Sgd1p; Essential nuclear protein with a possible role in the osmoregulatory glycerol response; interacts with phospholipase C (Plc1p); putative homolog of human NOM1 which is implicated in acute myeloid leukemia complement(799697..802396) Saccharomyces cerevisiae 851049 NP_013441.1 CDS VRP1 NC_001144.4 802653 805106 R Vrp1p; Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) complement(802653..805106) Saccharomyces cerevisiae 851051 NP_013444.1 CDS RPP0 NC_001144.4 805887 806825 D Rpp0p; Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302 805887..806825 Saccharomyces cerevisiae 851052 NP_013445.1 CDS SPO77 NC_001144.4 807385 808818 D Spo77p; Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis 807385..808818 Saccharomyces cerevisiae 851054 NP_013446.1 CDS FKS1 NC_001144.4 809997 815627 D Fks1p; Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling 809997..815627 Saccharomyces cerevisiae 851055 NP_878133.1 CDS YLR342W-A NC_001144.4 815810 815983 D Ylr342w-ap; Putative protein of unknown function 815810..815983 Saccharomyces cerevisiae 1466421 NP_013447.1 CDS GAS2 NC_001144.4 816094 817761 D Gas2p; 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p 816094..817761 Saccharomyces cerevisiae 851056 NP_013448.1 CDS RPL26A NC_001144.4 819312 820142 D Rpl26ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Bp and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA join(819312..819330,819778..820142) Saccharomyces cerevisiae 851058 NP_013449.1 CDS YLR345W NC_001144.4 820511 822040 D Ylr345wp; Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene 820511..822040 Saccharomyces cerevisiae 851059 NP_013450.1 CDS YLR346C NC_001144.4 822287 822592 R Ylr346cp; Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene complement(822287..822592) Saccharomyces cerevisiae 851060 NP_013451.1 CDS KAP95 NC_001144.4 823827 826412 R Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complex; Kap95p complement(823827..826412) Saccharomyces cerevisiae 851061 NP_013452.1 CDS DIC1 NC_001144.4 826976 827872 R Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix; Dic1p complement(826976..827872) Saccharomyces cerevisiae 851063 NP_013454.1 CDS ORM2 NC_001144.4 828729 829379 D Orm2p; Evolutionarily conserved protein with similarity to Orm1p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum 828729..829379 Saccharomyces cerevisiae 851064 NP_013455.1 CDS NIT3 NC_001144.4 829488 830363 R Nit3p; Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member complement(829488..830363) Saccharomyces cerevisiae 851065 NP_013456.1 CDS YLR352W NC_001144.4 831115 833538 D Ylr352wp; Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene 831115..833538 Saccharomyces cerevisiae 851066 NP_013457.1 CDS BUD8 NC_001144.4 834351 836162 D Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; Bud8p 834351..836162 Saccharomyces cerevisiae 851067 NP_013458.1 CDS TAL1 NC_001144.4 836349 837356 R Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; Tal1p complement(836349..837356) Saccharomyces cerevisiae 851068 NP_013459.1 CDS ILV5 NC_001144.4 838065 839252 R Ilv5p; Acetohydroxyacid reductoisomerase, mitochondrial protein involved in branched-chain amino acid biosynthesis, also required for maintenance of wild-type mitochondrial DNA and found in mitochondrial nucleoids complement(838065..839252) Saccharomyces cerevisiae 851069 NP_013460.1 CDS YLR356W NC_001144.4 840320 840913 D Ylr356wp; Putative protein of unknown function with similarity to SCM4; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR356W is not an essential gene 840320..840913 Saccharomyces cerevisiae 851070 NP_013461.1 CDS RSC2 NC_001144.4 841330 843999 D Rsc2p; Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance 841330..843999 Saccharomyces cerevisiae 851071 NP_013463.1 CDS ADE13 NC_001144.4 844281 845729 D Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency; Ade13p 844281..845729 Saccharomyces cerevisiae 851073 NP_013464.1 CDS VPS38 NC_001144.4 846102 847421 D Vps38p; Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity 846102..847421 Saccharomyces cerevisiae 851074 NP_013465.1 CDS DCR2 NC_001144.4 847387 849123 R Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START; Dcr2p complement(847387..849123) Saccharomyces cerevisiae 851075 NP_878134.1 CDS YLR361C-A NC_001144.4 849382 849678 R Ylr361c-ap; Putative protein of unknown function complement(849382..849678) Saccharomyces cerevisiae 1466422 NP_013466.1 CDS STE11 NC_001144.4 849865 852018 D Ste11p; Signal transducing MEK kinase involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p 849865..852018 Saccharomyces cerevisiae 851076 NP_013467.1 CDS NMD4 NC_001144.4 852494 853150 R Protein interacting with Nam7p, may be involved in the nonsense-mediated mRNA decay pathway; Nmd4p complement(852494..853150) Saccharomyces cerevisiae 851077 NP_076903.1 CDS YLR363W-A NC_001144.4 853460 853717 D Ylr363w-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 853460..853717 Saccharomyces cerevisiae 851078 NP_013468.1 CDS YLR364W NC_001144.4 854061 854390 D Ylr364wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR364W is not an essential gene 854061..854390 Saccharomyces cerevisiae 851079 NP_013471.1 CDS RPS22B NC_001144.4 856441 857316 D Rps22bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Ap and has similarity to E. coli S8 and rat S15a ribosomal proteins join(856441..856573,857057..857316) Saccharomyces cerevisiae 851082 NP_013472.1 CDS MDM30 NC_001144.4 857539 859335 D Mdm30p; F-box protein; physically associates with mitochondria and is required for normal mitochondrial fusion in rich medium, during sporulation, and in mating cells; promotes ubiquitin-mediated degradation of Gal4p in some strains 857539..859335 Saccharomyces cerevisiae 851083 NP_013473.1 CDS SSQ1 NC_001144.4 859551 861524 D Ssq1p; Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia 859551..861524 Saccharomyces cerevisiae 851084 NP_013474.1 CDS ARC18 NC_001144.4 861717 862253 R Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; Arc18p complement(861717..862253) Saccharomyces cerevisiae 851085 NP_013475.1 CDS ROM2 NC_001144.4 862713 866783 D Rom2p; GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP 862713..866783 Saccharomyces cerevisiae 851086 NP_013476.1 CDS SUR4 NC_001144.4 867353 868390 D Sur4p; Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis 867353..868390 Saccharomyces cerevisiae 851087 NP_013477.1 CDS VID22 NC_001144.4 868661 871366 R Vid22p; Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles complement(868661..871366) Saccharomyces cerevisiae 851088 NP_013479.1 CDS STP3 NC_001144.4 871696 872727 D Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; Stp3p 871696..872727 Saccharomyces cerevisiae 851089 NP_013480.1 CDS PSY3 NC_001144.4 872825 873553 R Psy3p; Protein of unknown function; deletion results in a mutator phenotype suggesting a role for this protein as a mutational suppressor; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C complement(872825..873553) Saccharomyces cerevisiae 851091 NP_013481.1 CDS FBP1 NC_001144.4 873745 874791 R Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; Fbp1p complement(873745..874791) Saccharomyces cerevisiae 851092 NP_013482.1 CDS SEC61 NC_001144.4 875735 877177 R Sec61p; Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER complement(875735..877177) Saccharomyces cerevisiae 851095 NP_013484.1 CDS CSR1 NC_001144.4 878282 879508 D Phosphatidylinositol transfer protein with a potential role in lipid turnover; interacts specifically with thioredoxin peroxidase (Tsa2p) and may have a role in oxidative stress resistance; Csr1p 878282..879508 Saccharomyces cerevisiae 851096 NP_013485.1 CDS CTF3 NC_001144.4 879723 881924 D Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; Ctf3p 879723..881924 Saccharomyces cerevisiae 851097 NP_013486.1 CDS NAM2 NC_001144.4 882067 884751 R Nam2p; Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance complement(882067..884751) Saccharomyces cerevisiae 851098 NP_013487.1 CDS SMC6 NC_001144.4 885288 888632 D Smc6p; Protein involved in structural maintenance of chromosomes; essential subunit of Mms21-Smc5-Smc6 complex; required for growth, DNA repair, interchromosomal and sister chromatid recombination; homologous to S. pombe rad18 885288..888632 Saccharomyces cerevisiae 851099 NP_013488.1 CDS IKI3 NC_001144.4 888851 892900 R Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD); Iki3p complement(888851..892900) Saccharomyces cerevisiae 851100 NP_013489.1 CDS SWC7 NC_001144.4 892992 893390 R Swc7p; Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin complement(892992..893390) Saccharomyces cerevisiae 851101 NP_013490.1 CDS VAC14 NC_001144.4 893628 896270 D Vac14p; Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p 893628..896270 Saccharomyces cerevisiae 851102 NP_013491.1 CDS REH1 NC_001144.4 896374 897672 R Reh1p; Protein of unknown function, similar to Rei1p but not involved in bud growth; contains dispersed C2H2 zinc finger domains complement(896374..897672) Saccharomyces cerevisiae 851103 NP_013492.1 CDS RPS29A NC_001144.4 898651 898821 D Rps29ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similarity to rat S29 and E. coli S14 ribosomal proteins 898651..898821 Saccharomyces cerevisiae 851104 NP_013493.2 CDS STE23 NC_001144.4 899577 902660 R Ste23p; Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; member of the insulin-degrading enzyme family complement(899577..902660) Saccharomyces cerevisiae 851105 NP_013494.1 CDS ECM19 NC_001144.4 903066 903404 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Ecm19p 903066..903404 Saccharomyces cerevisiae 851106 NP_013495.1 CDS CCW14 NC_001144.4 903724 904440 D Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall; Ccw14p 903724..904440 Saccharomyces cerevisiae 851107 NP_013496.1 CDS YLR392C NC_001144.4 904749 906305 R Ylr392cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR392C is not an essential gene complement(904749..906305) Saccharomyces cerevisiae 851108 NP_013497.1 CDS ATP10 NC_001144.4 907079 907918 D Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6; Atp10p 907079..907918 Saccharomyces cerevisiae 851109 NP_013498.1 CDS CST9 NC_001144.4 907950 909398 D SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate; Cst9p 907950..909398 Saccharomyces cerevisiae 851110 NP_013499.1 CDS COX8 NC_001144.4 909729 909965 R Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; Cox8p complement(909729..909965) Saccharomyces cerevisiae 851111 NP_013500.1 CDS VPS33 NC_001144.4 910235 912310 R Vps33p; ATP-binding protein that is a subunit of the HOPS complex and the CORVET tethering complex; essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport complement(910235..912310) Saccharomyces cerevisiae 851112 NP_013501.1 CDS AFG2 NC_001144.4 912550 914892 R Afg2p; ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; may be involved in degradation of aberrant mRNAs complement(912550..914892) Saccharomyces cerevisiae 851113 NP_013502.1 CDS SKI2 NC_001144.4 915156 919019 R Ski2p; Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses complement(915156..919019) Saccharomyces cerevisiae 851114 NP_013503.1 CDS BDF1 NC_001144.4 919536 921596 R Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; Bdf1p complement(919536..921596) Saccharomyces cerevisiae 851115 NP_013505.2 CDS DUS3 NC_001144.4 922442 924448 R Dus3p; Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region complement(922442..924448) Saccharomyces cerevisiae 851117 NP_013507.1 CDS SFP1 NC_001144.4 925567 927618 D Transcription factor that controls expression of many ribosome biogenesis genes in response to nutrients and stress, regulates G2/M transitions during mitotic cell cycle and DNA-damage response, involved in cell size modulation; Sfp1p 925567..927618 Saccharomyces cerevisiae 851119 NP_013508.1 CDS SEI1 NC_001144.4 928742 929599 D Seipin protein involved in lipid droplet morphology; null mutants form lipid droplet clusters, especially in stationary phase, which appear to bud chaotically from the ER; corresponding gene in humans (BSCL2) causes lipodystrophy; Sei1p 928742..929599 Saccharomyces cerevisiae 851120 NP_013509.1 CDS DUS4 NC_001144.4 929788 930891 D Dus4p; Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p 929788..930891 Saccharomyces cerevisiae 851121 NP_013510.1 CDS RPL31B NC_001144.4 931064 931754 R Rpl31bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p complement(join(931064..931348,931698..931754)) Saccharomyces cerevisiae 851122 NP_878135.1 CDS YLR406C-A NC_001144.4 932205 932354 R Ylr406c-ap; Putative protein of unknown function complement(932205..932354) Saccharomyces cerevisiae 1466423 NP_013511.1 CDS YLR407W NC_001144.4 932966 933655 D Ylr407wp; Putative protein of unknown function; YLR407W is not an essential gene 932966..933655 Saccharomyces cerevisiae 851123 NP_013512.1 CDS YLR408C NC_001144.4 933883 934251 R Ylr408cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene complement(933883..934251) Saccharomyces cerevisiae 851124 NP_013513.1 CDS UTP21 NC_001144.4 934412 937231 R Utp21p; Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data complement(934412..937231) Saccharomyces cerevisiae 851125 NP_013514.1 CDS VIP1 NC_001144.4 937539 940979 D Vip1p; Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of IP7 by Vip1p is important for phosphate signaling; likely involved in cortical actin cytoskeleton function, by analogy with S. pombe ortholog asp1 937539..940979 Saccharomyces cerevisiae 851126 NP_058174.1 CDS YLR410W-B NC_001144.4 941481 946794 D Ylr410w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(941481..942773,942775..946794) Saccharomyces cerevisiae 851128 NP_058175.1 CDS YLR410W-A NC_001144.4 941481 942797 D Ylr410w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 941481..942797 Saccharomyces cerevisiae 851127 NP_013515.1 CDS CTR3 NC_001144.4 947251 947976 D High-affinity copper transporter of the plasma membrane, acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae; Ctr3p 947251..947976 Saccharomyces cerevisiae 851129 NP_013516.1 CDS YLR412W NC_001144.4 948366 949190 D Ylr412wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene 948366..949190 Saccharomyces cerevisiae 851130 NP_878136.1 CDS YLR412C-A NC_001144.4 950264 950470 R Ylr412c-ap; Putative protein of unknown function complement(950264..950470) Saccharomyces cerevisiae 1466424 NP_013517.1 CDS YLR413W NC_001144.4 951153 953180 D Ylr413wp; Putative protein of unknown function; YLR413W is not an essential gene 951153..953180 Saccharomyces cerevisiae 851131 NP_013518.1 CDS YLR414C NC_001144.4 953350 954141 R Ylr414cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the bud and cytoplasm; Hog1p is required for transcriptional induction in response to cell wall damage; YLR414C is not an essential gene complement(953350..954141) Saccharomyces cerevisiae 851132 NP_013519.1 CDS YLR415C NC_001144.4 954256 954594 R Ylr415cp; Putative protein of unknown function; YLR415C is not an essential gene complement(954256..954594) Saccharomyces cerevisiae 851133 NP_013521.1 CDS VPS36 NC_001144.4 955007 956707 D Vps36p; Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome 955007..956707 Saccharomyces cerevisiae 851135 NP_013522.1 CDS CDC73 NC_001144.4 956911 958092 R Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinct from Srb-containing Pol II complexes; required for expression of certain genes, modification of some histones, and telomere maintenance; Cdc73p complement(956911..958092) Saccharomyces cerevisiae 851136 NP_013523.1 CDS YLR419W NC_001144.4 958425 962732 D Ylr419wp; Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene 958425..962732 Saccharomyces cerevisiae 851137 NP_013524.1 CDS URA4 NC_001144.4 963782 964876 D Ura4p; Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate 963782..964876 Saccharomyces cerevisiae 851139 NP_013525.1 CDS RPN13 NC_001144.4 965087 965557 R Subunit of the 19S regulatory particle of the 26S proteasome lid; Rpn13p complement(965087..965557) Saccharomyces cerevisiae 851140 NP_013526.1 CDS YLR422W NC_001144.4 965894 971692 D Ylr422wp; Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein 965894..971692 Saccharomyces cerevisiae 851141 NP_013527.1 CDS ATG17 NC_001144.4 971914 973167 R Scaffold potein responsible for pre-autophagosomal structure organization; interacts with and is required for activation of Apg1p protein kinase; involved in autophagy but not in the Cvt (cytoplasm to vacuole targeting) pathway; Atg17p complement(971914..973167) Saccharomyces cerevisiae 851142 NP_013528.1 CDS SPP382 NC_001144.4 973392 975518 D Spp382p; Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation 973392..975518 Saccharomyces cerevisiae 851143 NP_013529.1 CDS TUS1 NC_001144.4 982891 986814 D Tus1p; Guanine nucleotide exchange factor (GEF) that functions to modulate Rho1p activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate 982891..986814 Saccharomyces cerevisiae 851145 NP_013530.1 CDS YLR426W NC_001144.4 987059 988110 D Ylr426wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin join(987059..987138,987210..988110) Saccharomyces cerevisiae 851146 NP_013531.1 CDS MAG2 NC_001144.4 988425 990437 D Mag2p; Cytoplasmic protein of unknown function predicted to encode a DNA-3-methyladenine glycosidase II that catalyzes the hydrolysis of alkylated DNA 988425..990437 Saccharomyces cerevisiae 851147 NP_013533.1 CDS CRN1 NC_001144.4 990774 992729 D Crn1p; Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity 990774..992729 Saccharomyces cerevisiae 851148 NP_013534.1 CDS SEN1 NC_001144.4 993431 1000126 D Sen1p; Presumed helicase required for RNA polymerase II transcription termination and processing of RNAs; homolog of Senataxin which causes Ataxia-Oculomotor Apraxia 2 and a dominant form of amyotrophic lateral sclerosis 993431..1000126 Saccharomyces cerevisiae 851150 NP_013535.1 CDS ATG23 NC_001144.4 1000339 1001700 R Peripheral membrane protein, required for autophagy and for the cytoplasm-to-vacuole targeting (Cvt) pathway; Atg23p complement(1000339..1001700) Saccharomyces cerevisiae 851151 NP_013536.1 CDS IMD3 NC_001144.4 1002554 1004125 D Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed; Imd3p 1002554..1004125 Saccharomyces cerevisiae 851152 NP_013537.1 CDS CNA1 NC_001144.4 1004344 1006005 R Cna1p; Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 complement(1004344..1006005) Saccharomyces cerevisiae 851153 NP_013539.2 CDS TSR2 NC_001144.4 1006375 1006992 D Tsr2p; Protein with a potential role in pre-rRNA processing 1006375..1006992 Saccharomyces cerevisiae 851154 NP_013540.1 CDS ECM30 NC_001144.4 1007418 1011242 R Non-essential protein of unknown function; Ecm30p complement(1007418..1011242) Saccharomyces cerevisiae 851156 NP_013541.1 CDS YLR437C NC_001144.4 1011619 1012020 R Ylr437cp; Putative protein of unknown function; epitope tagged protein localizes to the cytoplasm complement(1011619..1012020) Saccharomyces cerevisiae 851157 NP_013542.1 CDS CAR2 NC_001144.4 1012498 1013772 D L-ornithine transaminase (OTAse), catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; Car2p 1012498..1013772 Saccharomyces cerevisiae 851158 NP_013543.1 CDS LSM3 NC_001144.4 1013906 1014175 R Lsm3p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA complement(1013906..1014175) Saccharomyces cerevisiae 851159 NP_013544.1 CDS MRPL4 NC_001144.4 1014488 1015447 D Mrpl4p; Mitochondrial ribosomal protein of the large subunit 1014488..1015447 Saccharomyces cerevisiae 851160 NP_013545.1 CDS SEC39 NC_001144.4 1015565 1017694 R Sec39p; Protein of unknown function proposed to be involved in protein secretion; interacts with Dsl1p and localizes to the ER and nuclear envelope complement(1015565..1017694) Saccharomyces cerevisiae 851161 NP_013546.1 CDS RPS1A NC_001144.4 1018138 1018905 R Rps1ap; Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein complement(1018138..1018905) Saccharomyces cerevisiae 851162 NP_013547.1 CDS SIR3 NC_001144.4 1019312 1022248 R Sir3p; Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p complement(1019312..1022248) Saccharomyces cerevisiae 851163 NP_013548.1 CDS ECM7 NC_001144.4 1022622 1023968 D Non-essential protein of unknown function; Ecm7p 1022622..1023968 Saccharomyces cerevisiae 851164 NP_013550.2 CDS YLR445W NC_001144.4 1024186 1024834 D Ylr445wp; Putative protein of unknown function; transcription is regulated by Ume6p and induced in response to alpha factor join(1024186..1024569,1024652..1024834) Saccharomyces cerevisiae 851166 NP_013551.1 CDS YLR446W NC_001144.4 1025211 1026512 D Ylr446wp; Putative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene 1025211..1026512 Saccharomyces cerevisiae 851167 NP_013552.1 CDS VMA6 NC_001144.4 1026853 1027890 R Vma6p; Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membrane complement(1026853..1027890) Saccharomyces cerevisiae 851168 NP_013553.1 CDS RPL6B NC_001144.4 1028850 1029764 D Rpl6bp; Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Ap and to rat L6 ribosomal protein; binds to 5.8S rRNA join(1028850..1028864,1029249..1029764) Saccharomyces cerevisiae 851169 NP_013554.1 CDS FPR4 NC_001144.4 1030830 1032008 D Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; Fpr4p 1030830..1032008 Saccharomyces cerevisiae 851170 NP_013555.1 CDS HMG2 NC_001144.4 1032624 1035761 D One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; Hmg2p 1032624..1035761 Saccharomyces cerevisiae 851171 NP_013556.1 CDS LEU3 NC_001144.4 1036090 1038750 D Zinc-finger transcription factor that regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; positively regulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis; Leu3p 1036090..1038750 Saccharomyces cerevisiae 851172 NP_013557.1 CDS SST2 NC_001144.4 1039268 1041364 R Sst2p; GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family complement(1039268..1041364) Saccharomyces cerevisiae 851173 NP_013558.1 CDS RIF2 NC_001144.4 1041797 1042984 R Rif2p; Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation complement(1041797..1042984) Saccharomyces cerevisiae 851174 NP_013559.1 CDS FMP27 NC_001144.4 1043996 1051882 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp27p 1043996..1051882 Saccharomyces cerevisiae 851175 NP_013560.1 CDS YLR455W NC_001144.4 1053627 1054541 D Ylr455wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) 1053627..1054541 Saccharomyces cerevisiae 851177 NP_013561.1 CDS YLR456W NC_001144.4 1055068 1055682 D Ylr456wp; Putative protein of unknown function 1055068..1055682 Saccharomyces cerevisiae 851178 NP_013562.1 CDS NBP1 NC_001144.4 1055810 1056769 R Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; Nbp1p complement(1055810..1056769) Saccharomyces cerevisiae 851180 NP_013564.1 CDS GAB1 NC_001144.4 1057332 1058516 D Gab1p; GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI 1057332..1058516 Saccharomyces cerevisiae 851181 NP_013565.1 CDS YLR460C NC_001144.4 1059755 1060885 R Ylr460cp; Putative protein of unknown function, possibly up-regulated by iodine complement(1059755..1060885) Saccharomyces cerevisiae 851182 NP_013566.1 CDS PAU4 NC_001144.4 1062917 1063279 D Pau4p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme 1062917..1063279 Saccharomyces cerevisiae 851183 NP_013567.1 CDS YLR462W NC_001144.4 1065954 1066562 D Ylr462wp; Putative protein of unknown function with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII 1065954..1066562 Saccharomyces cerevisiae 851184 NP_013569.1 CDS YLR464W NC_001144.4 1066570 1067499 D Ylr464wp; Putative protein of unknown function; intron is predicted but not detected experimentally; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C join(1066570..1067082,1067362..1067499) Saccharomyces cerevisiae 851185 NP_013571.1 CDS YRF1-4 NC_001144.4 1067085 1071233 D Yrf1-4p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 1067085..1071233 Saccharomyces cerevisiae 851187 NP_878137.1 CDS YLR466C-B NC_001144.4 1071592 1071708 R Ylr466c-bp; Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data complement(1071592..1071708) Saccharomyces cerevisiae 1466425 NP_013572.1 CDS YRF1-5 NC_001144.4 1072506 1077896 D Yrf1-5p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p 1072506..1077896 Saccharomyces cerevisiae 851189 NP_013573.1 CDS YML133C NC_001145.2 461 4684 R Yml133cp; Putative protein of unknown function with similarity to helicases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron complement(join(461..3791,3891..4684)) Saccharomyces cerevisiae 854906 NP_013574.1 CDS COS3 NC_001145.2 7244 8383 D Protein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos3p 7244..8383 Saccharomyces cerevisiae 854907 NP_013575.1 CDS YML131W NC_001145.2 10199 11296 D Yml131wp; Putative protein of unknown function with similarity to oxidoreductases; HOG1 and SKO1-dependent mRNA expression is induced after osmotic shock; GFP-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS 10199..11296 Saccharomyces cerevisiae 854908 NP_013576.1 CDS ERO1 NC_001145.2 11484 13175 R Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum; Ero1p complement(11484..13175) Saccharomyces cerevisiae 854909 NP_013577.1 CDS COX14 NC_001145.2 14542 14754 R Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; Cox14p complement(14542..14754) Saccharomyces cerevisiae 854910 NP_013578.1 CDS MSC1 NC_001145.2 15136 16677 R Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated; Msc1p complement(15136..16677) Saccharomyces cerevisiae 854911 NP_013579.1 CDS RSC9 NC_001145.2 17065 18810 D Rsc9p; Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway 17065..18810 Saccharomyces cerevisiae 854912 NP_013580.1 CDS ERG13 NC_001145.2 19061 20536 R Erg13p; 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis complement(19061..20536) Saccharomyces cerevisiae 854913 NP_013581.1 CDS PGA3 NC_001145.2 20762 21700 R Pga3p; Essential protein required for maturation of Gas1p and Pho8p, protein trafficking; GFP-fusion protein localizes to the endoplasmic reticulum; null mutants have a cell separation defect complement(20762..21700) Saccharomyces cerevisiae 854914 NP_013582.1 CDS TUB3 NC_001145.2 22049 23684 R Tub3p; Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p complement(join(22049..23361,23660..23684)) Saccharomyces cerevisiae 854915 NP_013583.1 CDS PHO84 NC_001145.2 24038 25801 R Pho84p; High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p complement(24038..25801) Saccharomyces cerevisiae 854916 NP_013585.1 CDS GTR1 NC_001145.2 26930 27862 D Gtr1p; Cytoplasmic GTP binding protein and negative regulator, with homolog Gtr2p, of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport; has homology to human RagA and RagB 26930..27862 Saccharomyces cerevisiae 854918 NP_013586.1 CDS NDI1 NC_001145.2 28266 29807 R Ndi1p; NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID complement(28266..29807) Saccharomyces cerevisiae 854919 NP_013587.1 CDS YML119W NC_001145.2 30611 31684 D Yml119wp; Putative protein of unknown funtion; YML119W is not an essential gene; potential Cdc28p substrate 30611..31684 Saccharomyces cerevisiae 854920 NP_013588.1 CDS NGL3 NC_001145.2 32334 33851 D Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p; Ngl3p 32334..33851 Saccharomyces cerevisiae 854921 NP_013589.1 CDS NAB6 NC_001145.2 34243 37647 D Putative RNA-binding protein, based on computational analysis of large-scale protein-protein interaction data; Nab6p 34243..37647 Saccharomyces cerevisiae 854922 NP_013591.1 CDS ATR1 NC_001145.2 38196 39824 D Multidrug efflux pump of the major facilitator superfamily, required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; Atr1p 38196..39824 Saccharomyces cerevisiae 854924 NP_013592.1 CDS VAN1 NC_001145.2 40187 41794 R Van1p; Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant complement(40187..41794) Saccharomyces cerevisiae 854925 NP_013593.1 CDS TAF8 NC_001145.2 42043 43575 R Taf8p; TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation complement(42043..43575) Saccharomyces cerevisiae 854926 NP_013594.1 CDS DAT1 NC_001145.2 44045 44791 D Dat1p; DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability 44045..44791 Saccharomyces cerevisiae 854927 NP_013595.1 CDS CTK3 NC_001145.2 45063 45953 D Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; Ctk3p 45063..45953 Saccharomyces cerevisiae 854928 NP_013596.1 CDS BUL2 NC_001145.2 46942 49704 D Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1; Bul2p 46942..49704 Saccharomyces cerevisiae 854929 NP_013597.1 CDS COQ5 NC_001145.2 50031 50954 R Coq5p; 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes complement(50031..50954) Saccharomyces cerevisiae 854930 NP_013598.1 CDS ZDS2 NC_001145.2 51640 54468 D Zds2p; Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; paralog of Zds1p 51640..54468 Saccharomyces cerevisiae 854931 NP_013599.1 CDS YML108W NC_001145.2 54793 55110 D Yml108wp; Putative protein of unknown function whose structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene 54793..55110 Saccharomyces cerevisiae 854863 NP_013600.2 CDS PML39 NC_001145.2 55265 56269 R Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes; Pml39p complement(55265..56269) Saccharomyces cerevisiae 854864 NP_013601.1 CDS URA5 NC_001145.2 56773 57453 D Ura5p; One of two orotate phosphoribosyltransferase isozymes (see also URA10) that catalyze the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate 56773..57453 Saccharomyces cerevisiae 854865 NP_013602.1 CDS SEC65 NC_001145.2 57866 58687 R Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19; Sec65p complement(57866..58687) Saccharomyces cerevisiae 854866 NP_013603.1 CDS MDM1 NC_001145.2 58939 62322 R Mdm1p; Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) complement(58939..62322) Saccharomyces cerevisiae 854867 NP_013604.1 CDS NUP188 NC_001145.2 62582 67549 R Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p; Nup188p complement(62582..67549) Saccharomyces cerevisiae 854868 NP_013605.1 CDS CAC2 NC_001145.2 68294 69700 D Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized histones onto recently replicated DNA, required for building functional kinetochores, conserved from yeast to humans; Cac2p 68294..69700 Saccharomyces cerevisiae 854869 NP_013607.1 CDS CUE4 NC_001145.2 69735 70088 R Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; Cue4p complement(69735..70088) Saccharomyces cerevisiae 854871 NP_878138.1 CDS YML100W-A NC_001145.2 70138 70311 D Yml100w-ap; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 70138..70311 Saccharomyces cerevisiae 1466496 NP_013608.1 CDS TSL1 NC_001145.2 70624 73920 D Tsl1p; Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function 70624..73920 Saccharomyces cerevisiae 854872 NP_013610.1 CDS ARG81 NC_001145.2 74398 77040 R Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p; Arg81p complement(74398..77040) Saccharomyces cerevisiae 854874 NP_013611.1 CDS TAF13 NC_001145.2 77267 77770 D Taf13p; TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors 77267..77770 Saccharomyces cerevisiae 854875 NP_013612.1 CDS VPS9 NC_001145.2 78335 79690 R Vps9p; A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin complement(78335..79690) Saccharomyces cerevisiae 854876 NP_013613.1 CDS YML096W NC_001145.2 79909 81486 D Yml096wp; Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 79909..81486 Saccharomyces cerevisiae 854877 NP_013614.1 CDS RAD10 NC_001145.2 81481 82113 R Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein; Rad10p complement(81481..82113) Saccharomyces cerevisiae 854878 NP_013616.1 CDS GIM5 NC_001145.2 82275 82849 D Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it; Gim5p join(82275..82290,82374..82849) Saccharomyces cerevisiae 854879 NP_013617.1 CDS UTP14 NC_001145.2 83090 85789 D Utp14p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA 83090..85789 Saccharomyces cerevisiae 854881 NP_013618.1 CDS PRE8 NC_001145.2 85987 86739 R Pre8p; 20S proteasome beta-type subunit complement(85987..86739) Saccharomyces cerevisiae 854882 NP_013619.1 CDS RPM2 NC_001145.2 87123 90731 R Rpm2p; Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus complement(87123..90731) Saccharomyces cerevisiae 854883 NP_013622.1 CDS UFO1 NC_001145.2 92235 94241 D Ufo1p; F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation 92235..94241 Saccharomyces cerevisiae 854886 NP_013623.1 CDS AIM33 NC_001145.2 94431 95369 R Putative protein of unknown function; null mutant displays increased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim33p complement(94431..95369) Saccharomyces cerevisiae 854887 NP_013624.1 CDS ALO1 NC_001145.2 95791 97371 R D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of D-erythroascorbic acid, which is protective against oxidative stress; Alo1p complement(95791..97371) Saccharomyces cerevisiae 854888 NP_013625.1 CDS TUB1 NC_001145.2 97941 99400 R Tub1p; Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules complement(join(97941..99259,99376..99400)) Saccharomyces cerevisiae 854889 NP_013627.1 CDS YML083C NC_001145.2 99794 101050 R Yml083cp; Putative protein of unknown function; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions complement(99794..101050) Saccharomyces cerevisiae 854891 NP_013628.1 CDS YML082W NC_001145.2 101862 103811 D Yml082wp; Putative protein predicted to have carbon-sulfur lyase activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YNML082W is not an essential gene 101862..103811 Saccharomyces cerevisiae 854892 NP_013629.1 CDS ATP18 NC_001145.2 103983 104162 R Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms; Atp18p complement(103983..104162) Saccharomyces cerevisiae 854893 NP_013630.1 CDS YML081W NC_001145.2 104777 108532 D Yml081wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YML081w is not an essential gene 104777..108532 Saccharomyces cerevisiae 854894 NP_013631.1 CDS DUS1 NC_001145.2 108806 110077 D Dus1p; Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 108806..110077 Saccharomyces cerevisiae 854895 NP_013632.1 CDS YML079W NC_001145.2 110247 110852 D Yml079wp; Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm 110247..110852 Saccharomyces cerevisiae 854896 NP_013633.1 CDS CPR3 NC_001145.2 111002 111550 D Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria; Cpr3p 111002..111550 Saccharomyces cerevisiae 854897 NP_013634.1 CDS BET5 NC_001145.2 111865 112344 D Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi; Bet5p 111865..112344 Saccharomyces cerevisiae 854898 NP_013635.1 CDS WAR1 NC_001145.2 112513 115347 R War1p; Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively complement(112513..115347) Saccharomyces cerevisiae 854899 NP_013636.1 CDS HMG1 NC_001145.2 115734 118898 R One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae; Hmg1p complement(115734..118898) Saccharomyces cerevisiae 854900 NP_013637.1 CDS FPR3 NC_001145.2 120089 121324 R Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; Fpr3p complement(120089..121324) Saccharomyces cerevisiae 854901 NP_013638.1 CDS RPL6A NC_001145.2 123227 124172 R Rpl6ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA complement(join(123227..123742,124158..124172)) Saccharomyces cerevisiae 854902 NP_013639.1 CDS TCB3 NC_001145.2 124730 129367 R Tcb3p; Lipid-binding protein, localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; GFP-fusion protein localizes to the cell periphery; C-termini of Tcb1p, Tcb2p and Tcb3p interact complement(124730..129367) Saccharomyces cerevisiae 854903 NP_013640.1 CDS COG8 NC_001145.2 129749 131572 R Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog8p complement(129749..131572) Saccharomyces cerevisiae 854904 NP_013641.1 CDS DAK1 NC_001145.2 133475 135229 D Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation; Dak1p 133475..135229 Saccharomyces cerevisiae 854932 NP_013642.1 CDS POB3 NC_001145.2 135500 137158 D Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which facilitates RNA Pol II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure; interacts with DNA polymerase alpha (Pol1p); Pob3p 135500..137158 Saccharomyces cerevisiae 854933 NP_013643.1 CDS ITT1 NC_001145.2 137550 138944 D Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins; Itt1p 137550..138944 Saccharomyces cerevisiae 854934 NP_013644.1 CDS ERV41 NC_001145.2 139063 140214 R Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein; Erv41p complement(join(139063..140090,140184..140214)) Saccharomyces cerevisiae 854935 NP_013645.1 CDS SMA2 NC_001145.2 140424 141533 R Sma2p; Protein of unknown function involved in the assembly of the prospore membrane during sporulation complement(140424..141533) Saccharomyces cerevisiae 854936 NP_013646.1 CDS ORC1 NC_001145.2 142210 144954 D Orc1p; Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity 142210..144954 Saccharomyces cerevisiae 854937 NP_013647.1 CDS TEM1 NC_001145.2 145139 145876 R Tem1p; GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis complement(145139..145876) Saccharomyces cerevisiae 854938 NP_013648.1 CDS RPS1B NC_001145.2 146482 147249 D Rps1bp; Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein 146482..147249 Saccharomyces cerevisiae 854939 NP_013649.1 CDS MFT1 NC_001145.2 147505 148683 R Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance; Mft1p complement(147505..148683) Saccharomyces cerevisiae 854940 NP_013650.1 CDS PIF1 NC_001145.2 148953 151532 R Pif1p; DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA complement(148953..151532) Saccharomyces cerevisiae 854941 NP_013651.1 CDS OGG1 NC_001145.2 151871 153001 D Ogg1p; Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA 151871..153001 Saccharomyces cerevisiae 854942 NP_013652.1 CDS NTE1 NC_001145.2 153219 158258 R Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine; Nte1p complement(153219..158258) Saccharomyces cerevisiae 854943 NP_219498.1 CDS HUG1 NC_001145.2 158760 158966 D Protein involved in the Mec1p-mediated checkpoint pathway that responds to DNA damage or replication arrest, transcription is induced by DNA damage; Hug1p 158760..158966 Saccharomyces cerevisiae 854944 NP_013653.1 CDS SML1 NC_001145.2 159383 159697 D Sml1p; Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase 159383..159697 Saccharomyces cerevisiae 854945 NP_013655.1 CDS CMP2 NC_001145.2 160180 161994 D Cmp2p; Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 160180..161994 Saccharomyces cerevisiae 854946 NP_013656.1 CDS IMD4 NC_001145.2 162194 164176 R Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed; Imd4p complement(join(162194..163308,163717..164176)) Saccharomyces cerevisiae 854948 NP_013657.1 CDS SPC2 NC_001145.2 164790 165326 D Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; Spc2p 164790..165326 Saccharomyces cerevisiae 854949 NP_013658.1 CDS CYB2 NC_001145.2 165533 167308 R Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions; Cyb2p complement(165533..167308) Saccharomyces cerevisiae 854950 NP_878139.1 CDS YML054C-A NC_001145.2 167623 167781 R Yml054c-ap; Putative protein of unknown function complement(167623..167781) Saccharomyces cerevisiae 1466497 NP_013659.1 CDS YML053C NC_001145.2 169116 169754 R Yml053cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YML053C is not an essential gene complement(169116..169754) Saccharomyces cerevisiae 854952 NP_013660.1 CDS SUR7 NC_001145.2 170402 171310 D Sur7p; Putative integral membrane protein; component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants 170402..171310 Saccharomyces cerevisiae 854953 NP_013661.1 CDS GAL80 NC_001145.2 171594 172901 D Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding; Gal80p 171594..172901 Saccharomyces cerevisiae 854954 NP_013662.1 CDS AIM32 NC_001145.2 173139 174074 D Putative protein of unknown function; non-essential gene; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim32p 173139..174074 Saccharomyces cerevisiae 854955 NP_013663.1 CDS RSE1 NC_001145.2 174220 178305 R Rse1p; Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport complement(174220..178305) Saccharomyces cerevisiae 854956 NP_013664.1 CDS GSF2 NC_001145.2 178426 179637 D Gsf2p; ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression 178426..179637 Saccharomyces cerevisiae 854957 NP_013666.1 CDS PRM6 NC_001145.2 180017 181075 R Prm6p; Pheromone-regulated protein, predicted to have 2 transmembrane segments; regulated by Ste12p during mating complement(180017..181075) Saccharomyces cerevisiae 854959 NP_013667.1 CDS PRP39 NC_001145.2 181474 183363 D U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats; Prp39p 181474..183363 Saccharomyces cerevisiae 854960 NP_013668.1 CDS YML045W NC_001145.2 184461 189729 D Yml045wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(184461..185765,185767..189729) Saccharomyces cerevisiae 854963 NP_058176.1 CDS YML045W-A NC_001145.2 184461 185783 D Yml045w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 184461..185783 Saccharomyces cerevisiae 854962 NP_013669.1 CDS RRN11 NC_001145.2 190244 191767 R Rrn11p; Protein required for rDNA transcription by RNA polymerase I, component of the core factor (CF) of rDNA transcription factor, which also contains Rrn6p and Rrn7p complement(190244..191767) Saccharomyces cerevisiae 854964 NP_013670.1 CDS CAT2 NC_001145.2 192788 194800 D Cat2p; Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes 192788..194800 Saccharomyces cerevisiae 854965 NP_013671.1 CDS VPS71 NC_001145.2 194913 195755 R Vps71p; Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting complement(194913..195755) Saccharomyces cerevisiae 854966 NP_013672.1 CDS YML039W NC_001145.2 196628 201896 D Yml039wp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(196628..197932,197934..201896) Saccharomyces cerevisiae 854969 NP_013673.1 CDS YML040W NC_001145.2 196628 197950 D Yml040wp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 196628..197950 Saccharomyces cerevisiae 854968 NP_013674.1 CDS YMD8 NC_001145.2 202775 204103 R Ymd8p; Putative nucleotide sugar transporter, has similarity to Vrg4p complement(202775..204103) Saccharomyces cerevisiae 854970 NP_013675.1 CDS YML037C NC_001145.2 204386 205408 R Yml037cp; Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene complement(204386..205408) Saccharomyces cerevisiae 854971 NP_013676.2 CDS CGI121 NC_001145.2 205642 206293 D Protein involved in telomere uncapping and elongation as component of the KEOPS protein complex with Bud32p, Kae1p, Pcc1p, and Gon7p; also shown to be a component of the EKC protein complex; homolog of human CGI-121; Cgi121p join(205642..206097,206204..206293) Saccharomyces cerevisiae 854972 NP_013677.1 CDS AMD1 NC_001145.2 206428 208860 R Amd1p; AMP deaminase, tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; may be involved in regulation of intracellular adenine nucleotide pools complement(206428..208860) Saccharomyces cerevisiae 854973 NP_013679.1 CDS SRC1 NC_001145.2 209525 212155 D Src1p; Inner nuclear membrane (INM) protein with a putative role in sister chromatid segregation, potentially phosphorylated by Cdc28p; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence join(209525..211444,211571..212155) Saccharomyces cerevisiae 854974 NP_013680.2 CDS RAD52 NC_001145.2 212515 213930 R Rad52p; Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis complement(212515..213930) Saccharomyces cerevisiae 854976 NP_013681.1 CDS NDC1 NC_001145.2 214189 216156 D Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II; Ndc1p 214189..216156 Saccharomyces cerevisiae 854977 NP_013682.1 CDS AIM31 NC_001145.2 216435 216914 D Putative protein of unknown function; GFP-fusion protein localizes to mitochondria; null mutant is viable and displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth rate in minimal glycerol media; Aim31p 216435..216914 Saccharomyces cerevisiae 854978 NP_013683.1 CDS USA1 NC_001145.2 217362 219878 D Usa1p; Protein involved in ER-associated protein degradation (ERAD); component of the Hrd1p complex; interacts with the U1 snRNP-specific protein, Snp1p 217362..219878 Saccharomyces cerevisiae 854979 NP_013684.1 CDS TSA1 NC_001145.2 220138 220728 D Ubiquitous housekeeping thioredoxin peroxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; mediates redox regulation of the nuclear localization of Yap1p; deletion results in mutator phenotype; Tsa1p 220138..220728 Saccharomyces cerevisiae 854980 NP_013685.1 CDS YOX1 NC_001145.2 221406 222563 D Yox1p; Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate 221406..222563 Saccharomyces cerevisiae 854981 NP_013686.1 CDS RPS18B NC_001145.2 222987 223828 R Rps18bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins complement(join(222987..223380,223782..223828)) Saccharomyces cerevisiae 854982 NP_013687.1 CDS YML6 NC_001145.2 224406 225365 R Yml6p; Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins complement(join(224406..225239,225339..225365)) Saccharomyces cerevisiae 854983 NP_013688.1 CDS RPS17A NC_001145.2 225889 226697 D Rps17ap; Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein join(225889..225891,226290..226697) Saccharomyces cerevisiae 854984 NP_013689.1 CDS NSE5 NC_001145.2 226994 228664 R Nse5p; Essential subunit of the Mms21-Smc5-Smc6 complex; required for cell viability and DNA repair complement(226994..228664) Saccharomyces cerevisiae 854985 NP_013690.1 CDS APT1 NC_001145.2 228937 229500 D Apt1p; Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis 228937..229500 Saccharomyces cerevisiae 854986 NP_013691.1 CDS UNG1 NC_001145.2 229734 230813 R Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus; Ung1p complement(229734..230813) Saccharomyces cerevisiae 854987 NP_013692.1 CDS YML020W NC_001145.2 231149 233143 D Yml020wp; Putative protein of unknown function 231149..233143 Saccharomyces cerevisiae 854988 NP_013693.1 CDS OST6 NC_001145.2 233457 234455 D Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p; Ost6p 233457..234455 Saccharomyces cerevisiae 854989 NP_013694.1 CDS YML018C NC_001145.2 234771 235952 R Yml018cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; YML018C is not an essential gene complement(234771..235952) Saccharomyces cerevisiae 854990 NP_013695.2 CDS PSP2 NC_001145.2 236588 238731 D Psp2p; Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing join(236588..236591,236954..238731) Saccharomyces cerevisiae 854991 NP_013696.1 CDS PPZ1 NC_001145.2 239458 241536 R Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance; Ppz1p complement(239458..241536) Saccharomyces cerevisiae 854992 NP_013697.1 CDS TAF11 NC_001145.2 241989 243029 R Taf11p; TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors complement(241989..243029) Saccharomyces cerevisiae 854993 NP_013698.1 CDS TRM9 NC_001145.2 243225 244064 D tRNA methyltransferase, catalyzes the esterification of modified uridine nucleotides in tRNAs, creating 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu); may have a role in stress response; Trm9p 243225..244064 Saccharomyces cerevisiae 854994 NP_013699.1 CDS UBX2 NC_001145.2 244149 245903 D Ubx2p; Protein involved in ER-associated protein degradation; proposed to coordinate the assembly of proteins involved in ERAD; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain 244149..245903 Saccharomyces cerevisiae 854995 NP_013701.1 CDS ERV25 NC_001145.2 246116 246751 D Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport; Erv25p 246116..246751 Saccharomyces cerevisiae 854997 NP_013702.1 CDS RAD33 NC_001145.2 246895 247428 R Rad33p; Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus complement(246895..247428) Saccharomyces cerevisiae 854998 NP_013703.1 CDS SPT5 NC_001145.2 247677 250868 D Spt5p; Protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation; Spt4p-Spt5p complex also plays a role in pre-mRNA processing 247677..250868 Saccharomyces cerevisiae 854999 NP_013705.1 CDS MRPL39 NC_001145.2 251304 251516 R Mrpl39p; Mitochondrial ribosomal protein of the large subunit complement(251304..251516) Saccharomyces cerevisiae 855002 NP_013706.1 CDS ERG6 NC_001145.2 251839 252990 R Erg6p; Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane complement(251839..252990) Saccharomyces cerevisiae 855003 NP_076904.1 CDS YML007C-A NC_001145.2 253162 253272 R Yml007c-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria complement(253162..253272) Saccharomyces cerevisiae 855004 NP_013707.1 CDS YAP1 NC_001145.2 253848 255800 D Yap1p; Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; mediates resistance to cadmium 253848..255800 Saccharomyces cerevisiae 855005 NP_013708.1 CDS GIS4 NC_001145.2 256092 258416 R Gis4p; CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway complement(256092..258416) Saccharomyces cerevisiae 855006 NP_013709.1 CDS TRM12 NC_001145.2 260221 261609 D Trm12p; S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA 260221..261609 Saccharomyces cerevisiae 855008 NP_013710.1 CDS GLO1 NC_001145.2 261705 262685 R Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress; Glo1p complement(261705..262685) Saccharomyces cerevisiae 855009 NP_013711.1 CDS YML003W NC_001145.2 263483 264355 D Yml003wp; Putative protein of unknown function 263483..264355 Saccharomyces cerevisiae 855010 NP_013712.1 CDS YML002W NC_001145.2 264541 266754 D Yml002wp; Putative protein of unknown function; expression induced by heat and by calcium shortage 264541..266754 Saccharomyces cerevisiae 855011 NP_013713.1 CDS YPT7 NC_001145.2 267174 267800 D Ypt7p; GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, similar to mammalian Rab7 267174..267800 Saccharomyces cerevisiae 855012 NP_013714.1 CDS CDC5 NC_001145.2 269019 271136 R Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate; Cdc5p complement(269019..271136) Saccharomyces cerevisiae 855013 NP_878140.1 CDS YMR001C-A NC_001145.2 271347 271577 R Ymr001c-ap; Putative protein of unknown function complement(271347..271577) Saccharomyces cerevisiae 1466498 NP_013715.1 CDS MIC17 NC_001145.2 272193 272663 D Mitochondrial intermembrane space cysteine motif protein; MIC17 is not an essential gene; Mic17p 272193..272663 Saccharomyces cerevisiae 855014 NP_013716.1 CDS AIM34 NC_001145.2 273118 273714 D Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim34p 273118..273714 Saccharomyces cerevisiae 855015 NP_013717.1 CDS MVP1 NC_001145.2 274017 275552 D Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; Mvp1p 274017..275552 Saccharomyces cerevisiae 855016 NP_013718.1 CDS TAF4 NC_001145.2 276045 277211 D Taf4p; TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate 276045..277211 Saccharomyces cerevisiae 855017 NP_013719.1 CDS PLB2 NC_001145.2 277561 279681 R Plb2p; Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine complement(277561..279681) Saccharomyces cerevisiae 855018 NP_013721.1 CDS PLB1 NC_001145.2 280590 282584 R Plb1p; Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol complement(280590..282584) Saccharomyces cerevisiae 855020 NP_013722.1 CDS ADI1 NC_001145.2 284101 284640 D Adi1p; Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions 284101..284640 Saccharomyces cerevisiae 855021 NP_013723.1 CDS YMR010W NC_001145.2 285099 286316 D Ymr010wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 285099..286316 Saccharomyces cerevisiae 855022 NP_013724.1 CDS HXT2 NC_001145.2 288078 289703 D Hxt2p; High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose 288078..289703 Saccharomyces cerevisiae 855023 NP_013725.1 CDS CLU1 NC_001145.2 291133 294966 D eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; Clu1p 291133..294966 Saccharomyces cerevisiae 855025 NP_013726.1 CDS SEC59 NC_001145.2 295178 296737 R Sec59p; Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation complement(295178..296737) Saccharomyces cerevisiae 855026 NP_013727.1 CDS BUD22 NC_001145.2 298867 300426 D Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud22p 298867..300426 Saccharomyces cerevisiae 855028 NP_013728.1 CDS ERG5 NC_001145.2 300868 302484 R Erg5p; C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs complement(300868..302484) Saccharomyces cerevisiae 855029 NP_013729.1 CDS SOK2 NC_001145.2 303235 305592 R Sok2p; Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors complement(303235..305592) Saccharomyces cerevisiae 855030 NP_013730.1 CDS SPO20 NC_001145.2 307488 308681 D Spo20p; Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog 307488..308681 Saccharomyces cerevisiae 855031 NP_013731.1 CDS YMR018W NC_001145.2 310207 311751 D Ymr018wp; Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene 310207..311751 Saccharomyces cerevisiae 855032 NP_013732.1 CDS STB4 NC_001145.2 312155 315004 D Stb4p; Protein that binds Sin3p in a two-hybrid assay; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins 312155..315004 Saccharomyces cerevisiae 855033 NP_013733.1 CDS FMS1 NC_001145.2 315376 316902 D Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis; Fms1p 315376..316902 Saccharomyces cerevisiae 855034 NP_013734.1 CDS MAC1 NC_001145.2 317164 318417 R Mac1p; Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport complement(317164..318417) Saccharomyces cerevisiae 855035 NP_013735.1 CDS UBC7 NC_001145.2 318679 319176 D Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly; Ubc7p 318679..319176 Saccharomyces cerevisiae 855036 NP_013736.1 CDS MSS1 NC_001145.2 319436 321016 R Mss1p; Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 complement(319436..321016) Saccharomyces cerevisiae 855037 NP_013737.1 CDS MRPL3 NC_001145.2 321874 323046 D Mrpl3p; Mitochondrial ribosomal protein of the large subunit 321874..323046 Saccharomyces cerevisiae 855039 NP_013738.1 CDS CSI1 NC_001145.2 323299 324186 D Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; Csi1p 323299..324186 Saccharomyces cerevisiae 855040 NP_013739.1 CDS PEX12 NC_001145.2 324235 325434 R Pex12p; C3HC4-type RING-finger peroxisomal membrane peroxin required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorders complement(324235..325434) Saccharomyces cerevisiae 855041 NP_013740.1 CDS YMR027W NC_001145.2 325876 327288 D Ymr027wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene 325876..327288 Saccharomyces cerevisiae 855042 NP_013741.1 CDS TAP42 NC_001145.2 327481 328581 D Tap42p; Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits 327481..328581 Saccharomyces cerevisiae 855043 NP_013742.1 CDS FAR8 NC_001145.2 328659 330230 R Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p; Far8p complement(328659..330230) Saccharomyces cerevisiae 855044 NP_013743.1 CDS RSF1 NC_001145.2 330792 331922 D Rsf1p; Protein required for respiratory growth; localized to both the nucleus and mitochondrion; mutant displays decreased transcription of specific nuclear and mitochondrial genes whose products are involved in respiratory growth 330792..331922 Saccharomyces cerevisiae 855045 NP_013744.1 CDS YMR031C NC_001145.2 332211 334742 R Ymr031cp; Protein of unknown function with similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; YMR031C is not an essential gene complement(332211..334742) Saccharomyces cerevisiae 855047 NP_013746.1 CDS HOF1 NC_001145.2 335297 337306 D Hof1p; Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p 335297..337306 Saccharomyces cerevisiae 855048 NP_878141.1 CDS YMR030W-A NC_001145.2 337312 337602 D Ymr030w-ap; Putative protein of unknown function 337312..337602 Saccharomyces cerevisiae 1466499 NP_013747.1 CDS ARP9 NC_001145.2 337787 339276 D Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation; Arp9p join(337787..337816,337903..339276) Saccharomyces cerevisiae 855049 NP_013748.1 CDS YMR034C NC_001145.2 339417 340721 R Ymr034cp; Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene complement(339417..340721) Saccharomyces cerevisiae 855050 NP_013749.1 CDS IMP2 NC_001145.2 341141 341674 D Imp2p; Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p 341141..341674 Saccharomyces cerevisiae 855051 NP_013750.1 CDS MIH1 NC_001145.2 341855 343519 R Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25; Mih1p complement(341855..343519) Saccharomyces cerevisiae 855052 NP_013751.1 CDS MSN2 NC_001145.2 344402 346516 R Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; Msn2p complement(344402..346516) Saccharomyces cerevisiae 855053 NP_013752.1 CDS CCS1 NC_001145.2 347510 348259 R Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; Ccs1p complement(347510..348259) Saccharomyces cerevisiae 855054 NP_013753.1 CDS SUB1 NC_001145.2 348643 349521 R Transcriptional coactivator, facilitates elongation by influencing enzymes that modify RNAP II, acts in a peroxide resistance pathway involving Rad2p; suppressor of TFIIB mutations; Sub1p complement(348643..349521) Saccharomyces cerevisiae 855055 NP_013754.1 CDS YET2 NC_001145.2 350380 350862 D Yet2p; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein 350380..350862 Saccharomyces cerevisiae 855056 NP_013755.1 CDS ARA2 NC_001145.2 350965 351972 R NAD-dependent arabinose dehydrogenase, involved in biosynthesis of erythroascorbic acid; similar to plant L-galactose dehydrogenase; Ara2p complement(350965..351972) Saccharomyces cerevisiae 855057 NP_013756.1 CDS ARG80 NC_001145.2 352602 353135 D Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p; Arg80p 352602..353135 Saccharomyces cerevisiae 855059 NP_013757.1 CDS MCM1 NC_001145.2 353870 354730 D Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; Mcm1p 353870..354730 Saccharomyces cerevisiae 855060 NP_013758.1 CDS IOC4 NC_001145.2 355383 356810 D Member of a complex (Isw1b) with Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PWWP motif; Ioc4p 355383..356810 Saccharomyces cerevisiae 855061 NP_013759.1 CDS YMR045C NC_001145.2 357358 362626 R Ymr045cp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(357358..361320,361322..362626)) Saccharomyces cerevisiae 855062 NP_013760.1 CDS YMR046C NC_001145.2 361304 362626 R Ymr046cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(361304..362626) Saccharomyces cerevisiae 855063 NP_013762.1 CDS NUP116 NC_001145.2 363363 366704 R Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup100p; Nup116p complement(363363..366704) Saccharomyces cerevisiae 855066 NP_013763.1 CDS CSM3 NC_001145.2 366980 367933 D Protein required for accurate chromosome segregation during meiosis; Csm3p 366980..367933 Saccharomyces cerevisiae 855067 NP_013764.1 CDS ERB1 NC_001145.2 368093 370516 R Protein required for maturation of the 25S and 5.8S ribosomal RNAs; constituent of 66S pre-ribosomal particles; homologous to mammalian Bop1; Erb1p complement(368093..370516) Saccharomyces cerevisiae 855068 NP_013765.1 CDS YMR050C NC_001145.2 373056 378324 R Ymr050cp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(373056..377018,377020..378324)) Saccharomyces cerevisiae 855070 NP_013766.1 CDS YMR051C NC_001145.2 377002 378324 R Ymr051cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(377002..378324) Saccharomyces cerevisiae 855071 NP_013767.1 CDS FAR3 NC_001145.2 379585 380199 D Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; Far3p 379585..380199 Saccharomyces cerevisiae 855073 NP_013769.1 CDS STB2 NC_001145.2 380345 382897 R Stb2p; Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p complement(380345..382897) Saccharomyces cerevisiae 855075 NP_013770.1 CDS STV1 NC_001145.2 383302 385974 D Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole; Stv1p 383302..385974 Saccharomyces cerevisiae 855076 NP_013771.1 CDS BUB2 NC_001145.2 386100 387020 R Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage; Bub2p complement(386100..387020) Saccharomyces cerevisiae 855077 NP_013772.1 CDS AAC1 NC_001145.2 387314 388243 R Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; Aac1p complement(387314..388243) Saccharomyces cerevisiae 855078 NP_013774.1 CDS FET3 NC_001145.2 388821 390731 D Fet3p; Ferro-O2-oxidoreductase required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases 388821..390731 Saccharomyces cerevisiae 855080 NP_013775.2 CDS SEN15 NC_001145.2 391098 391484 D Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen15p 391098..391484 Saccharomyces cerevisiae 855081 NP_013776.1 CDS SAM37 NC_001145.2 391531 392514 R Sam37p; Component of the mitochondrial outer membrane sorting and assembly machinery (SAM) complex; required for the sorting of some proteins to the outer membrane after import by the TOM complex complement(391531..392514) Saccharomyces cerevisiae 855082 NP_013777.1 CDS RNA14 NC_001145.2 392754 394787 D Rna14p; Cleavage and polyadenylation factor I (CF I) component involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex 392754..394787 Saccharomyces cerevisiae 855083 NP_013778.1 CDS ECM40 NC_001145.2 395053 396378 R Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine; Ecm40p complement(395053..396378) Saccharomyces cerevisiae 855084 NP_013779.1 CDS RIM9 NC_001145.2 397076 397795 D Rim9p; Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalI; putative membrane protein 397076..397795 Saccharomyces cerevisiae 855085 NP_013780.1 CDS AEP1 NC_001145.2 397902 399458 D Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase; Aep1p 397902..399458 Saccharomyces cerevisiae 855086 NP_013781.1 CDS KAR5 NC_001145.2 399701 401215 D Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone; Kar5p 399701..401215 Saccharomyces cerevisiae 855087 NP_013782.1 CDS SOV1 NC_001145.2 401540 404236 D Sov1p; Mitochondrial protein of unknown function 401540..404236 Saccharomyces cerevisiae 855088 NP_013783.1 CDS UBX4 NC_001145.2 404322 405572 R Ubx4p; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p complement(404322..405572) Saccharomyces cerevisiae 855089 NP_013784.1 CDS AVO2 NC_001145.2 406303 407583 D Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth; Avo2p 406303..407583 Saccharomyces cerevisiae 855090 NP_013785.1 CDS NAT4 NC_001145.2 407708 408565 D Nat4p; N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A 407708..408565 Saccharomyces cerevisiae 855091 NP_013786.1 CDS MOT3 NC_001145.2 409153 410625 D Nuclear transcription factor with two Cys2-His2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes; Mot3p 409153..410625 Saccharomyces cerevisiae 855092 NP_013787.1 CDS TVP18 NC_001145.2 410761 411264 R Tvp18p; Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments complement(410761..411264) Saccharomyces cerevisiae 855093 NP_013788.1 CDS ABF2 NC_001145.2 411568 412119 D Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; Abf2p 411568..412119 Saccharomyces cerevisiae 855094 NP_013789.1 CDS IRC21 NC_001145.2 412267 412872 R Putative protein of unknown function; proposed to be involved in resistance to carboplatin and cisplatin; shares similarity to a human cytochrome oxidoreductase; null mutant displays increased levels of spontaneous Rad52 foci; Irc21p complement(412267..412872) Saccharomyces cerevisiae 855095 NP_013790.1 CDS YMR074C NC_001145.2 413035 413472 R Ymr074cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR074C is not an essential gene complement(413035..413472) Saccharomyces cerevisiae 855096 NP_013791.1 CDS RCO1 NC_001145.2 413981 416035 D Rco1p; Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p 413981..416035 Saccharomyces cerevisiae 855097 NP_013793.1 CDS PDS5 NC_001145.2 416195 420028 R Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes in an interdependent manner, may function as a protein-protein interaction scaffold; Pds5p complement(416195..420028) Saccharomyces cerevisiae 855099 NP_013794.1 CDS VPS20 NC_001145.2 421483 422148 R Vps20p; Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes complement(421483..422148) Saccharomyces cerevisiae 855101 NP_013795.1 CDS CTF18 NC_001145.2 422502 424727 R Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint; Ctf18p complement(422502..424727) Saccharomyces cerevisiae 855102 NP_013796.1 CDS SEC14 NC_001145.2 424988 426058 D Sec14p; Phosphatidylinositol/phosphatidylcholine transfer protein involved in coordinate regulation of PtdIns and PtdCho metabolism, products of which are regulators in Golgi to plasma membrane transport; functionally homologous to mammalian PITPs join(424988..424996,425153..426058) Saccharomyces cerevisiae 855103 NP_013797.1 CDS NAM7 NC_001145.2 426711 429626 R Nam7p; ATP-dependent RNA helicase of the SFI superfamily, required for nonsense mediated mRNA decay and for efficient translation termination at nonsense codons; involved in telomere maintenance complement(426711..429626) Saccharomyces cerevisiae 855104 NP_013798.1 CDS ISF1 NC_001145.2 430078 431094 R Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; Isf1p complement(430078..431094) Saccharomyces cerevisiae 855105 NP_013800.1 CDS ADH3 NC_001145.2 434787 435914 D Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production; Adh3p 434787..435914 Saccharomyces cerevisiae 855107 NP_013801.1 CDS YMR084W NC_001145.2 436627 437415 D Ymr084wp; Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains 436627..437415 Saccharomyces cerevisiae 855108 NP_013802.1 CDS YMR085W NC_001145.2 437490 438788 D Ymr085wp; Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains 437490..438788 Saccharomyces cerevisiae 855109 NP_013803.1 CDS YMR086W NC_001145.2 439207 442089 D Ymr086wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; cAMP represses YMR086W expression 439207..442089 Saccharomyces cerevisiae 855110 NP_013805.1 CDS YMR087W NC_001145.2 442526 443380 D Ymr087wp; Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain 442526..443380 Saccharomyces cerevisiae 855112 NP_013806.1 CDS VBA1 NC_001145.2 443413 445101 R Vba1p; Permease of basic amino acids in the vacuolar membrane complement(443413..445101) Saccharomyces cerevisiae 855113 NP_013807.1 CDS YTA12 NC_001145.2 445608 448085 R Yta12p; Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes complement(445608..448085) Saccharomyces cerevisiae 855114 NP_013808.1 CDS YMR090W NC_001145.2 449244 449927 D Ymr090wp; Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR090W is not an essential gene 449244..449927 Saccharomyces cerevisiae 855115 NP_013809.1 CDS NPL6 NC_001145.2 450057 451364 R Npl6p; Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions; involved in nuclear protein import and maintenance of proper telomere length complement(450057..451364) Saccharomyces cerevisiae 855116 NP_013810.1 CDS AIP1 NC_001145.2 451631 453478 R Aip1p; Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats complement(451631..453478) Saccharomyces cerevisiae 855117 NP_013811.1 CDS UTP15 NC_001145.2 454014 455555 D Utp15p; Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA 454014..455555 Saccharomyces cerevisiae 855118 NP_013812.1 CDS CTF13 NC_001145.2 455824 457260 D Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis; Ctf13p 455824..457260 Saccharomyces cerevisiae 855119 NP_013813.1 CDS SNO1 NC_001145.2 457284 457958 R Sno1p; Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase complement(457284..457958) Saccharomyces cerevisiae 855120 NP_013814.1 CDS SNZ1 NC_001145.2 458407 459300 D Snz1p; Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins 458407..459300 Saccharomyces cerevisiae 855121 NP_013815.1 CDS MTG1 NC_001145.2 459423 460526 R Peripheral GTPase of the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals; Mtg1p complement(459423..460526) Saccharomyces cerevisiae 855122 NP_013816.1 CDS LRC4 NC_001145.2 460770 462608 R Protein of unknown function; non-tagged protein is detected in highly purified mitochondria; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Lrc4p complement(460770..462608) Saccharomyces cerevisiae 855123 NP_013817.1 CDS YMR099C NC_001145.2 463933 464826 R Ymr099cp; Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS complement(463933..464826) Saccharomyces cerevisiae 855125 NP_013818.1 CDS MUB1 NC_001145.2 466299 468161 D Protein of unknown function, deletion causes multi-budding phenotype; has similarity to Aspergillus nidulans samB gene; Mub1p 466299..468161 Saccharomyces cerevisiae 855126 NP_013819.1 CDS SRT1 NC_001145.2 468444 469475 R Srt1p; Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase complement(468444..469475) Saccharomyces cerevisiae 855127 NP_013820.1 CDS YMR102C NC_001145.2 469847 472351 R Ymr102cp; Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene complement(469847..472351) Saccharomyces cerevisiae 855128 NP_013822.1 CDS YPK2 NC_001145.2 473419 475452 R Ypk2p; Protein kinase with similarityto serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK complement(473419..475452) Saccharomyces cerevisiae 855130 NP_013823.1 CDS PGM2 NC_001145.2 475896 477605 R Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; Pgm2p complement(475896..477605) Saccharomyces cerevisiae 855131 NP_878142.1 CDS YMR105W-A NC_001145.2 478063 478257 D Ymr105w-ap; Putative protein of unknown function 478063..478257 Saccharomyces cerevisiae 1466500 NP_013824.1 CDS YKU80 NC_001145.2 478300 480189 R Yku80p; Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair complement(478300..480189) Saccharomyces cerevisiae 855132 NP_013825.1 CDS SPG4 NC_001145.2 483013 483360 D Spg4p; Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources 483013..483360 Saccharomyces cerevisiae 855134 NP_013826.1 CDS ILV2 NC_001145.2 484083 486146 D Ilv2p; Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control 484083..486146 Saccharomyces cerevisiae 855135 NP_013827.1 CDS MYO5 NC_001145.2 486586 490245 D One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; Myo5p 486586..490245 Saccharomyces cerevisiae 855136 NP_013828.1 CDS HFD1 NC_001145.2 490393 491991 R Putative fatty aldehyde dehydrogenase, located in the mitochondrial outer membrane and also in lipid particles; has similarity to human fatty aldehyde dehydrogenase (FALDH) which is implicated in Sjogren-Larsson syndrome; Hfd1p complement(490393..491991) Saccharomyces cerevisiae 855137 NP_013829.1 CDS YMR111C NC_001145.2 492403 493791 R Ymr111cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene complement(492403..493791) Saccharomyces cerevisiae 855138 NP_013830.1 CDS MED11 NC_001145.2 494099 494494 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein; Med11p complement(494099..494494) Saccharomyces cerevisiae 855139 NP_013831.1 CDS FOL3 NC_001145.2 494998 496281 D Fol3p; Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis 494998..496281 Saccharomyces cerevisiae 855140 NP_013832.1 CDS YMR114C NC_001145.2 496342 497448 R Ymr114cp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene complement(496342..497448) Saccharomyces cerevisiae 855141 NP_013833.1 CDS FMP24 NC_001145.2 497703 499208 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp24p 497703..499208 Saccharomyces cerevisiae 855142 NP_013834.1 CDS ASC1 NC_001145.2 499455 500687 R G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; represses Gcn4p in the absence of amino acid starvation; Asc1p complement(join(499455..499877,500151..500687)) Saccharomyces cerevisiae 855143 NP_013835.1 CDS SPC24 NC_001145.2 501249 501890 R Spc24p; Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering complement(501249..501890) Saccharomyces cerevisiae 855144 NP_013836.1 CDS YMR118C NC_001145.2 502143 502733 R Ymr118cp; Protein of unknown function with similarity to succinate dehydrogenase cytochrome b subunit; YMR118C is not an essential gene complement(502143..502733) Saccharomyces cerevisiae 855145 NP_013837.1 CDS ASI1 NC_001145.2 505332 507206 D Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; Asi1p 505332..507206 Saccharomyces cerevisiae 855147 NP_013839.1 CDS ADE17 NC_001145.2 507501 509279 R Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine; Ade17p complement(507501..509279) Saccharomyces cerevisiae 855149 NP_013840.1 CDS RPL15B NC_001145.2 509733 510347 R Rpl15bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA complement(509733..510347) Saccharomyces cerevisiae 855150 NP_061493.1 CDS YMR122W-A NC_001145.2 511314 511568 D Ymr122w-ap; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and endoplasmic reticulum 511314..511568 Saccharomyces cerevisiae 855152 NP_013842.1 CDS PKR1 NC_001145.2 513592 513960 D V-ATPase assembly factor, functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); overproduction confers resistance to Pichia farinosa killer toxin; Pkr1p 513592..513960 Saccharomyces cerevisiae 855153 NP_013843.1 CDS YMR124W NC_001145.2 514455 517286 D Ymr124wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene 514455..517286 Saccharomyces cerevisiae 855154 NP_013844.2 CDS STO1 NC_001145.2 517538 520445 D Sto1p; Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 join(517538..517562,517885..520445) Saccharomyces cerevisiae 855155 NP_013845.1 CDS DLT1 NC_001145.2 520760 521788 R Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA); Dlt1p complement(520760..521788) Saccharomyces cerevisiae 855156 NP_013846.1 CDS SAS2 NC_001145.2 522328 523344 R Sas2p; Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family complement(522328..523344) Saccharomyces cerevisiae 855157 NP_013847.1 CDS ECM16 NC_001145.2 523695 527498 D Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis; Ecm16p 523695..527498 Saccharomyces cerevisiae 855158 NP_013848.1 CDS POM152 NC_001145.2 527803 531816 D Pom152p; Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase 527803..531816 Saccharomyces cerevisiae 855159 NP_013849.1 CDS YMR130W NC_001145.2 532118 533026 D Ymr130wp; Putative protein of unknown function; YMR130W is not an essential gene 532118..533026 Saccharomyces cerevisiae 855160 NP_013850.1 CDS RRB1 NC_001145.2 533162 534697 R Rrb1p; Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p complement(533162..534697) Saccharomyces cerevisiae 855161 NP_013851.1 CDS JLP2 NC_001145.2 534943 535569 R Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein); Jlp2p complement(534943..535569) Saccharomyces cerevisiae 855162 NP_013852.1 CDS REC114 NC_001145.2 536206 537608 D Rec114p; Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division join(536206..537447,537564..537608) Saccharomyces cerevisiae 855163 NP_013853.1 CDS ERG29 NC_001145.2 537837 538550 D Protein that binds to and regulates Erg25p; localized to the ER; null mutant is viable in a respiratory defective background; synthetic lethal with mmt1 and mmt2 mutations; highly conserved in ascomycetes; Erg29p 537837..538550 Saccharomyces cerevisiae 855164 NP_013854.1 CDS GID8 NC_001145.2 538688 540055 R Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains LisH and CTLH domains, like Vid30p; dosage-dependent regulator of START; Gid8p complement(538688..540055) Saccharomyces cerevisiae 855166 NP_013856.1 CDS GAT2 NC_001145.2 541198 542880 D Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine; Gat2p 541198..542880 Saccharomyces cerevisiae 855167 NP_013857.1 CDS PSO2 NC_001145.2 542977 544962 R Required for a post-incision step in the repair of DNA single and double-strand breaks that result from interstrand crosslinks produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; Pso2p complement(542977..544962) Saccharomyces cerevisiae 855168 NP_013858.1 CDS CIN4 NC_001145.2 545154 545729 D GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; Cin4p 545154..545729 Saccharomyces cerevisiae 855169 NP_013859.1 CDS RIM11 NC_001145.2 546124 547236 D Rim11p; Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta 546124..547236 Saccharomyces cerevisiae 855170 NP_013860.1 CDS SIP5 NC_001145.2 547713 549182 D Sip5p; Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase 547713..549182 Saccharomyces cerevisiae 855171 NP_013862.1 CDS RPL13B NC_001145.2 550205 551206 R Rpl13bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein complement(join(550205..550800,551203..551206)) Saccharomyces cerevisiae 855173 NP_013863.2 CDS RPS16A NC_001145.2 551927 552902 D Rps16ap; Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins join(551927..551950,552495..552902) Saccharomyces cerevisiae 855174 NP_013864.1 CDS YMR144W NC_001145.2 553361 554389 D Ymr144wp; Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene 553361..554389 Saccharomyces cerevisiae 855175 NP_013865.1 CDS NDE1 NC_001145.2 554792 556474 R Nde1p; Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain complement(554792..556474) Saccharomyces cerevisiae 855176 NP_013866.1 CDS TIF34 NC_001145.2 557480 558523 R Tif34p; Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation complement(557480..558523) Saccharomyces cerevisiae 855177 NP_013867.1 CDS YMR147W NC_001145.2 559198 559869 D Ymr147wp; Putative protein of unknown function 559198..559869 Saccharomyces cerevisiae 855178 NP_013868.1 CDS YMR148W NC_001145.2 560365 560811 D Ymr148wp; Putative protein of unknown function; predicted to contain a transmembrane domain; YMR148W is not an essential gene 560365..560811 Saccharomyces cerevisiae 855179 NP_013869.1 CDS SWP1 NC_001145.2 560995 561855 D Swp1p; Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum 560995..561855 Saccharomyces cerevisiae 855180 NP_013870.1 CDS IMP1 NC_001145.2 561955 562527 R Imp1p; Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p complement(561955..562527) Saccharomyces cerevisiae 855182 NP_013872.1 CDS YIM1 NC_001145.2 563095 564192 D Yim1p; Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 563095..564192 Saccharomyces cerevisiae 855183 NP_013873.1 CDS NUP53 NC_001145.2 564434 565861 D Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex; Nup53p 564434..565861 Saccharomyces cerevisiae 855184 NP_013875.1 CDS RIM13 NC_001145.2 565998 568181 R Rim13p; Calpain-like cysteine protease involved in proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans palB complement(565998..568181) Saccharomyces cerevisiae 855186 NP_013876.1 CDS YMR155W NC_001145.2 568550 570193 D Ymr155wp; Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen 568550..570193 Saccharomyces cerevisiae 855187 NP_013877.1 CDS TPP1 NC_001145.2 570299 571015 R Tpp1p; DNA 3'-phosphatase that functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; has similarity to the l-2-haloacid dehalogenase superfamily complement(570299..571015) Saccharomyces cerevisiae 855188 NP_013878.1 CDS AIM36 NC_001145.2 571276 572043 R Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss and reduced growth rate in minimal glycerol media; Aim36p complement(571276..572043) Saccharomyces cerevisiae 855189 NP_013879.1 CDS MRPS8 NC_001145.2 572247 572714 D Mrps8p; Mitochondrial ribosomal protein of the small subunit 572247..572714 Saccharomyces cerevisiae 855190 NP_013880.1 CDS YMR158C-A NC_001145.2 573193 573330 R Ymr158c-ap; Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene complement(573193..573330) Saccharomyces cerevisiae 855192 NP_013882.1 CDS ATG16 NC_001145.2 574475 574927 R Protein that interacts with the Atg12p-Atg5p conjugate during formation of the pre-autophagosomal structure; essential for autophagy; Atg16p complement(574475..574927) Saccharomyces cerevisiae 855194 NP_013883.1 CDS YMR160W NC_001145.2 575065 577515 D Ymr160wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene 575065..577515 Saccharomyces cerevisiae 855195 NP_013884.1 CDS HLJ1 NC_001145.2 577717 578391 D Hlj1p; Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Hdj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ 577717..578391 Saccharomyces cerevisiae 855196 NP_013885.1 CDS DNF3 NC_001145.2 578950 583920 R Dnf3p; Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vessicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase complement(578950..583920) Saccharomyces cerevisiae 855197 NP_013886.1 CDS INP2 NC_001145.2 584270 586387 R Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene; Inp2p complement(584270..586387) Saccharomyces cerevisiae 855198 NP_013887.1 CDS MSS11 NC_001145.2 587273 589549 R Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of MUC1 and STA2 in response to nutritional signals; Mss11p complement(587273..589549) Saccharomyces cerevisiae 855200 NP_013888.1 CDS PAH1 NC_001145.2 590039 592627 R Pah1p; Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and Pi, responsible for de novo lipid synthesis; homologous to mammalian lipin 1 complement(590039..592627) Saccharomyces cerevisiae 855201 NP_013889.1 CDS YMR166C NC_001145.2 593366 594472 R Ymr166cp; Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene complement(593366..594472) Saccharomyces cerevisiae 855202 NP_013890.1 CDS MLH1 NC_001145.2 594885 597194 D Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer; Mlh1p 594885..597194 Saccharomyces cerevisiae 855203 NP_013891.1 CDS CEP3 NC_001145.2 597331 599157 R Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain; Cep3p complement(597331..599157) Saccharomyces cerevisiae 855204 NP_013892.1 CDS ALD3 NC_001145.2 599351 600871 R Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose; Ald3p complement(599351..600871) Saccharomyces cerevisiae 855205 NP_013893.1 CDS ALD2 NC_001145.2 601561 603081 R Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p; Ald2p complement(601561..603081) Saccharomyces cerevisiae 855206 NP_013894.1 CDS EAR1 NC_001145.2 603867 605519 R Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; mRNA is targeted to the bud via the mRNA transport system involving She2p: YMR171C is not an essential gene; Ear1p complement(603867..605519) Saccharomyces cerevisiae 855207 NP_013895.1 CDS HOT1 NC_001145.2 605980 608139 D Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p; Hot1p 605980..608139 Saccharomyces cerevisiae 855208 NP_013897.1 CDS DDR48 NC_001145.2 608688 609980 D Ddr48p; DNA damage-responsive protein, expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS 608688..609980 Saccharomyces cerevisiae 855210 NP_013899.1 CDS PAI3 NC_001145.2 610158 610364 R Pai3p; Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact complement(610158..610364) Saccharomyces cerevisiae 855212 NP_013900.1 CDS SIP18 NC_001145.2 611015 611254 D Sip18p; Protein of unknown function whose expression is induced by osmotic stress 611015..611254 Saccharomyces cerevisiae 855213 NP_878143.1 CDS YMR175W-A NC_001145.2 611313 611507 D Ymr175w-ap; Putative protein of unknown function 611313..611507 Saccharomyces cerevisiae 1466501 NP_013901.1 CDS ECM5 NC_001145.2 611739 615974 D Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; Ecm5p 611739..615974 Saccharomyces cerevisiae 855214 NP_013902.1 CDS MMT1 NC_001145.2 616565 618097 D Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p; Mmt1p 616565..618097 Saccharomyces cerevisiae 855215 NP_013903.1 CDS YMR178W NC_001145.2 618478 619302 D Ymr178wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR178W is not an essential gene 618478..619302 Saccharomyces cerevisiae 855216 NP_013904.1 CDS SPT21 NC_001145.2 619857 622133 D Spt21p; Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; involved in telomere maintenance 619857..622133 Saccharomyces cerevisiae 855217 NP_013905.1 CDS CTL1 NC_001145.2 622250 623212 R RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; Ctl1p complement(622250..623212) Saccharomyces cerevisiae 855218 NP_013906.1 CDS YMR181C NC_001145.2 623615 624079 R Ymr181cp; Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); YMR181C is not an essential gene complement(623615..624079) Saccharomyces cerevisiae 855219 NP_013907.1 CDS RGM1 NC_001145.2 624531 625166 R Rgm1p; Putative transcriptional repressor with proline-rich zinc fingers; overproduction impairs cell growth complement(624531..625166) Saccharomyces cerevisiae 855220 NP_878144.1 CDS YMR182W-A NC_001145.2 625810 625896 D Ymr182w-ap; Putative protein of unknown function 625810..625896 Saccharomyces cerevisiae 1466502 NP_013908.1 CDS SSO2 NC_001145.2 626920 627807 R Sso2p; Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p complement(626920..627807) Saccharomyces cerevisiae 855221 NP_013909.1 CDS ADD37 NC_001145.2 628188 628784 D Protein of unknown function involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; Add37p 628188..628784 Saccharomyces cerevisiae 855222 NP_013910.1 CDS YMR185W NC_001145.2 629024 631969 D Ymr185wp; Putative protein of unknown function; essential gene required for viability 629024..631969 Saccharomyces cerevisiae 855223 NP_013911.1 CDS HSC82 NC_001145.2 632354 634471 D Hsc82p; Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock 632354..634471 Saccharomyces cerevisiae 855224 NP_013912.1 CDS YMR187C NC_001145.2 634688 635983 R Ymr187cp; Putative protein of unknown function; YMR187C is not an essential gene complement(634688..635983) Saccharomyces cerevisiae 855225 NP_013913.1 CDS MRPS17 NC_001145.2 636290 637003 R Mrps17p; Mitochondrial ribosomal protein of the small subunit complement(636290..637003) Saccharomyces cerevisiae 855226 NP_013914.1 CDS GCV2 NC_001145.2 637499 640603 D P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm; Gcv2p 637499..640603 Saccharomyces cerevisiae 855227 NP_013915.1 CDS SGS1 NC_001145.2 640914 645257 R Sgs1p; Nucleolar DNA helicase of the RecQ family involved in maintenance of genome integrity, regulates chromosome synapsis and meiotic crossing over; has similarity to human BLM and WRN helicases implicated in Bloom and Werner syndromes complement(640914..645257) Saccharomyces cerevisiae 855228 NP_013916.2 CDS SPG5 NC_001145.2 645655 646776 D Spg5p; Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources 645655..646776 Saccharomyces cerevisiae 855229 NP_013917.1 CDS GYL1 NC_001145.2 647117 649279 D Putative GTPase activating protein (GAP) that may have a role in polarized exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; Gyl1p 647117..649279 Saccharomyces cerevisiae 855230 NP_013918.1 CDS MRPL24 NC_001145.2 650035 650811 D Mrpl24p; Mitochondrial ribosomal protein of the large subunit 650035..650811 Saccharomyces cerevisiae 855231 NP_013920.1 CDS RPL36A NC_001145.2 651144 651909 D Rpl36ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Bp and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA join(651144..651159,651623..651909) Saccharomyces cerevisiae 855232 NP_878145.1 CDS YMR194C-B NC_001145.2 652593 652886 R Ymr194c-bp; Putative protein of unknown function complement(join(652593..652774,652847..652886)) Saccharomyces cerevisiae 1466503 NP_013922.1 CDS ICY1 NC_001145.2 654033 654416 D Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; Icy1p 654033..654416 Saccharomyces cerevisiae 855235 NP_013923.1 CDS YMR196W NC_001145.2 655075 658341 D Ymr196wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene 655075..658341 Saccharomyces cerevisiae 855236 NP_013924.1 CDS VTI1 NC_001145.2 658544 659197 R Vti1p; Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways complement(658544..659197) Saccharomyces cerevisiae 855237 NP_013925.1 CDS CIK1 NC_001145.2 659744 661528 D Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p; Cik1p 659744..661528 Saccharomyces cerevisiae 855238 NP_013926.1 CDS CLN1 NC_001145.2 662643 664283 D G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); Cln1p 662643..664283 Saccharomyces cerevisiae 855239 NP_013927.1 CDS ROT1 NC_001145.2 664751 665521 D Rot1p; Essential ER membrane protein; may be involved in protein folding; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations 664751..665521 Saccharomyces cerevisiae 855240 NP_013928.1 CDS RAD14 NC_001145.2 665844 667043 R Rad14p; Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein complement(join(665844..666932,667017..667043)) Saccharomyces cerevisiae 855241 NP_013929.1 CDS ERG2 NC_001145.2 667536 668204 D Erg2p; C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis 667536..668204 Saccharomyces cerevisiae 855242 NP_013930.1 CDS TOM40 NC_001145.2 668491 669654 D Tom40p; Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore 668491..669654 Saccharomyces cerevisiae 855243 NP_013931.1 CDS INP1 NC_001145.2 670062 671324 R Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; Inp1p complement(670062..671324) Saccharomyces cerevisiae 855244 NP_013932.1 CDS PFK2 NC_001145.2 671886 674765 R Pfk2p; Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes complement(671886..674765) Saccharomyces cerevisiae 855245 NP_013933.1 CDS YMR206W NC_001145.2 675895 676836 D Ymr206wp; Putative protein of unknown function; YMR206W is not an essential gene 675895..676836 Saccharomyces cerevisiae 855246 NP_013934.1 CDS HFA1 NC_001145.2 677192 683563 R Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; Hfa1p complement(677192..683563) Saccharomyces cerevisiae 855247 NP_013935.1 CDS ERG12 NC_001145.2 684466 685797 D Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate; Erg12p 684466..685797 Saccharomyces cerevisiae 855248 NP_013936.1 CDS YMR209C NC_001145.2 685910 687283 R Ymr209cp; Putative protein of unknown function; YMR209C is not an essential gene complement(685910..687283) Saccharomyces cerevisiae 855249 NP_013937.1 CDS YMR210W NC_001145.2 687515 688864 D Ymr210wp; Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification 687515..688864 Saccharomyces cerevisiae 855250 NP_013938.1 CDS DML1 NC_001145.2 689082 690509 D Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family; Dml1p 689082..690509 Saccharomyces cerevisiae 855251 NP_013939.1 CDS EFR3 NC_001145.2 690694 693042 R Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies and is phosphorylated; Efr3p complement(690694..693042) Saccharomyces cerevisiae 855252 NP_013940.1 CDS CEF1 NC_001145.2 693380 695152 D Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p; Cef1p 693380..695152 Saccharomyces cerevisiae 855253 NP_013941.2 CDS SCJ1 NC_001145.2 695349 696482 D Scj1p; One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins 695349..696482 Saccharomyces cerevisiae 855254 NP_013942.1 CDS GAS3 NC_001145.2 696795 698369 D Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall; Gas3p 696795..698369 Saccharomyces cerevisiae 855255 NP_013943.1 CDS SKY1 NC_001145.2 698810 701038 R Sky1p; SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 complement(698810..701038) Saccharomyces cerevisiae 855256 NP_013944.1 CDS GUA1 NC_001145.2 701789 703366 D Gua1p; GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation 701789..703366 Saccharomyces cerevisiae 855257 NP_013945.1 CDS TRS130 NC_001145.2 703578 706886 R Trs130p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1 complement(703578..706886) Saccharomyces cerevisiae 855258 NP_013946.1 CDS ESC1 NC_001145.2 707132 712108 D Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p; Esc1p 707132..712108 Saccharomyces cerevisiae 855259 NP_013947.1 CDS ERG8 NC_001145.2 712315 713670 D Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate; Erg8p 712315..713670 Saccharomyces cerevisiae 855260 NP_013948.1 CDS FMP42 NC_001145.2 713930 715444 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp42p complement(713930..715444) Saccharomyces cerevisiae 855261 NP_013949.1 CDS FSH2 NC_001145.2 715637 716308 R Serine hydrolase that localizes to the cytoplasm; sequence is similar to Fsh1p and Fsh3p; Fsh2p complement(715637..716308) Saccharomyces cerevisiae 855262 NP_013950.1 CDS UBP8 NC_001145.2 716714 718129 D Ubp8p; Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B 716714..718129 Saccharomyces cerevisiae 855263 NP_013951.1 CDS MRE11 NC_001145.2 718574 720652 R Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved; Mre11p complement(718574..720652) Saccharomyces cerevisiae 855264 NP_013952.1 CDS MRPL44 NC_001145.2 720959 721402 R Mrpl44p; Mitochondrial ribosomal protein of the large subunit complement(join(720959..721197,721345..721402)) Saccharomyces cerevisiae 855265 NP_013953.1 CDS TMA29 NC_001145.2 721592 722395 R Tma29p; NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments complement(721592..722395) Saccharomyces cerevisiae 855266 NP_013954.1 CDS TAF7 NC_001145.2 722612 724384 R Taf7p; TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation complement(722612..724384) Saccharomyces cerevisiae 855267 NP_013955.1 CDS MTF1 NC_001145.2 724625 725650 D Mtf1p; Mitochondrial RNA polymerase specificity factor with structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors, interacts with mitochondrial core polymerase Rpo41p 724625..725650 Saccharomyces cerevisiae 855268 NP_013956.1 CDS RRP5 NC_001145.2 725933 731122 R Rrp5p; Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and N-terminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome complement(725933..731122) Saccharomyces cerevisiae 855269 NP_013957.1 CDS RPS10B NC_001145.2 732413 733140 D Rps10bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similarity to rat ribosomal protein S10 join(732413..732464,732875..733140) Saccharomyces cerevisiae 855270 NP_878146.1 CDS YMR230W-A NC_001145.2 733267 733455 D Ymr230w-ap; Putative protein of unknown function 733267..733455 Saccharomyces cerevisiae 1466504 NP_013958.1 CDS PEP5 NC_001145.2 733544 736633 D Pep5p; Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p 733544..736633 Saccharomyces cerevisiae 855271 NP_013959.1 CDS FUS2 NC_001145.2 736925 738958 D Fus2p; Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating 736925..738958 Saccharomyces cerevisiae 855272 NP_013960.1 CDS TRI1 NC_001145.2 739184 739864 D Tri1p; Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus 739184..739864 Saccharomyces cerevisiae 855273 NP_013961.1 CDS RNH1 NC_001145.2 740265 741311 D Rnh1p; Ribonuclease H1, removes RNA primers during Okazaki fragment synthesis; degrades RNA attached to the 5'-end of a DNA strand 740265..741311 Saccharomyces cerevisiae 855274 NP_013962.1 CDS RNA1 NC_001145.2 741511 742734 R Rna1p; GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport complement(741511..742734) Saccharomyces cerevisiae 855275 NP_013963.1 CDS TAF9 NC_001145.2 742970 743443 D Taf9p; Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 742970..743443 Saccharomyces cerevisiae 855276 NP_013964.1 CDS BCH1 NC_001145.2 743748 745922 D Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; Bch1p 743748..745922 Saccharomyces cerevisiae 855277 NP_013965.1 CDS DFG5 NC_001145.2 746352 747728 D Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p; Dfg5p 746352..747728 Saccharomyces cerevisiae 855278 NP_013966.1 CDS RNT1 NC_001145.2 748261 749676 R Rnt1p; RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end complement(748261..749676) Saccharomyces cerevisiae 855280 NP_013967.1 CDS CUS1 NC_001145.2 749929 751239 R Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p; Cus1p complement(749929..751239) Saccharomyces cerevisiae 855281 NP_013968.1 CDS YHM2 NC_001145.2 751960 752904 D Yhm2p; Mitochondrial DNA-binding protein, component of the mitochondrial nucleoid structure, involved in mtDNA replication and segregation of mitochondrial genomes; member of the mitochondrial carrier protein family 751960..752904 Saccharomyces cerevisiae 855282 NP_013969.3 CDS RPL20A NC_001145.2 753224 754219 R Rpl20ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similarity to rat L18a ribosomal protein complement(join(753224..753741,754219)) Saccharomyces cerevisiae 855283 NP_878147.1 CDS YMR242W-A NC_001145.2 754296 754385 D Ymr242w-ap; Putative protein of unknown function 754296..754385 Saccharomyces cerevisiae 1466505 NP_013970.1 CDS ZRC1 NC_001145.2 754837 756165 R Zrc1p; Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm complement(754837..756165) Saccharomyces cerevisiae 855284 NP_013971.1 CDS YMR244W NC_001145.2 757249 758316 D Ymr244wp; Putative protein of unknown function 757249..758316 Saccharomyces cerevisiae 855285 NP_013972.1 CDS YMR244C-A NC_001145.2 758516 758830 R Ymr244c-ap; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR244C-A is not an essential gene complement(758516..758830) Saccharomyces cerevisiae 855287 NP_013974.1 CDS FAA4 NC_001145.2 759806 761890 D Long chain fatty acyl-CoA synthetase, regulates protein modification during growth in the presence of ethanol, functions to incorporate palmitic acid into phospholipids and neutral lipids; Faa4p 759806..761890 Saccharomyces cerevisiae 855288 NP_013975.1 CDS RKR1 NC_001145.2 763350 768038 R Rkr1p; Nuclear RING domain protein with functional connections to chromatin modification; may interact with ribosomes, based on co-purification experiments; YMR247C is not an essential gene complement(763350..768038) Saccharomyces cerevisiae 855289 NP_878148.1 CDS YMR247W-A NC_001145.2 769282 769425 D Ymr247w-ap; Putative protein of unknown function 769282..769425 Saccharomyces cerevisiae 1466506 NP_013976.1 CDS GAD1 NC_001145.2 770800 772557 D Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress; Gad1p 770800..772557 Saccharomyces cerevisiae 855291 NP_013977.1 CDS GTO3 NC_001145.2 772914 774014 D Omega class glutathione transferase; putative cytosolic localization; Gto3p 772914..774014 Saccharomyces cerevisiae 855292 NP_013978.1 CDS HOR7 NC_001145.2 774751 774930 D Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; Hor7p 774751..774930 Saccharomyces cerevisiae 855293 NP_013979.1 CDS YMR252C NC_001145.2 775314 775718 R Ymr252cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene complement(775314..775718) Saccharomyces cerevisiae 855294 NP_013980.1 CDS YMR253C NC_001145.2 775945 777189 R Ymr253cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene complement(775945..777189) Saccharomyces cerevisiae 855295 NP_013982.1 CDS GFD1 NC_001145.2 778000 778566 D Gfd1p; Coiled-coiled protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation 778000..778566 Saccharomyces cerevisiae 855297 NP_013983.1 CDS COX7 NC_001145.2 778944 779126 R Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; Cox7p complement(778944..779126) Saccharomyces cerevisiae 855298 NP_013984.1 CDS PET111 NC_001145.2 779628 782030 R Pet111p; Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane complement(779628..782030) Saccharomyces cerevisiae 855299 NP_013985.1 CDS YMR258C NC_001145.2 782619 784280 R Ymr258cp; Protein of unknown function with similarity to F-box proteins; physically interacts with Skp1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR258C is not an essential gene complement(782619..784280) Saccharomyces cerevisiae 855300 NP_013986.1 CDS YMR259C NC_001145.2 784620 788882 R Ymr259cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene complement(784620..788882) Saccharomyces cerevisiae 855301 NP_013987.1 CDS TIF11 NC_001145.2 789377 789838 R Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3; Tif11p complement(789377..789838) Saccharomyces cerevisiae 855302 NP_013988.1 CDS TPS3 NC_001145.2 790204 793368 R Tps3p; Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway complement(790204..793368) Saccharomyces cerevisiae 855303 NP_013989.1 CDS YMR262W NC_001145.2 793725 794666 D Ymr262wp; Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene 793725..794666 Saccharomyces cerevisiae 855304 NP_013990.1 CDS SAP30 NC_001145.2 794918 795523 D Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance; Sap30p 794918..795523 Saccharomyces cerevisiae 855305 NP_013991.1 CDS CUE1 NC_001145.2 795804 796415 D Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; Cue1p 795804..796415 Saccharomyces cerevisiae 855306 NP_013992.1 CDS YMR265C NC_001145.2 796539 797924 R Ymr265cp; Putative protein of unknown function complement(796539..797924) Saccharomyces cerevisiae 855307 NP_013993.1 CDS RSN1 NC_001145.2 798517 801378 D Rsn1p; Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant 798517..801378 Saccharomyces cerevisiae 855308 NP_013994.1 CDS PPA2 NC_001145.2 801771 802703 D Ppa2p; Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate 801771..802703 Saccharomyces cerevisiae 855309 NP_013995.1 CDS PRP24 NC_001145.2 802887 804221 R Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling; Prp24p complement(802887..804221) Saccharomyces cerevisiae 855310 NP_013996.2 CDS TMA23 NC_001145.2 804455 805090 D Tma23p; Nucleolar protein of unknown function implicated in ribosome biogenesis; TMA23 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes 804455..805090 Saccharomyces cerevisiae 855311 NP_013997.1 CDS RRN9 NC_001145.2 805325 806422 R Rrn9p; Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I complement(805325..806422) Saccharomyces cerevisiae 855312 NP_013998.1 CDS URA10 NC_001145.2 806864 807547 R Ura10p; One of two orotate phosphoribosyltransferase isozymes (see also URA5) that catalyze the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate complement(806864..807547) Saccharomyces cerevisiae 855313 NP_013999.1 CDS SCS7 NC_001145.2 809622 810776 R Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth; Scs7p complement(809622..810776) Saccharomyces cerevisiae 855315 NP_878149.1 CDS YMR272W-B NC_001145.2 811088 811195 D Ymr272w-bp; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 811088..811195 Saccharomyces cerevisiae 1466507 NP_014000.1 CDS ZDS1 NC_001145.2 811232 813979 R Zds1p; Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A; implicated in mRNA nuclear export complement(811232..813979) Saccharomyces cerevisiae 855316 NP_014001.1 CDS RCE1 NC_001145.2 814363 815310 R Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone; Rce1p complement(814363..815310) Saccharomyces cerevisiae 855317 NP_014002.1 CDS BUL1 NC_001145.2 815650 818580 R Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; Bul1p complement(815650..818580) Saccharomyces cerevisiae 855318 NP_014003.1 CDS DSK2 NC_001145.2 818826 819947 D Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome; Dsk2p 818826..819947 Saccharomyces cerevisiae 855319 NP_014004.1 CDS FCP1 NC_001145.2 820255 822453 D Fcp1p; Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) 820255..822453 Saccharomyces cerevisiae 855320 NP_014005.1 CDS PGM3 NC_001145.2 822762 824630 D Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential; Pgm3p 822762..824630 Saccharomyces cerevisiae 855321 NP_014006.1 CDS YMR279C NC_001145.2 824728 826350 R Ymr279cp; Putative protein of unknown function; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene complement(824728..826350) Saccharomyces cerevisiae 855322 NP_014007.1 CDS CAT8 NC_001145.2 827027 831328 R Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; Cat8p complement(827027..831328) Saccharomyces cerevisiae 855323 NP_014008.1 CDS GPI12 NC_001145.2 832338 833252 D Gpi12p; ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp 832338..833252 Saccharomyces cerevisiae 855324 NP_014009.1 CDS AEP2 NC_001145.2 833355 835097 R Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader; Aep2p complement(833355..835097) Saccharomyces cerevisiae 855325 NP_014010.1 CDS RIT1 NC_001145.2 835325 836866 R Rit1p; 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA complement(835325..836866) Saccharomyces cerevisiae 855326 NP_014011.1 CDS YKU70 NC_001145.2 838186 839994 D Yku70p; Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair 838186..839994 Saccharomyces cerevisiae 855328 NP_014012.1 CDS NGL2 NC_001145.2 840143 841690 R Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p; Ngl2p complement(840143..841690) Saccharomyces cerevisiae 855329 NP_014013.1 CDS MRPL33 NC_001145.2 841941 842201 D Mrpl33p; Mitochondrial ribosomal protein of the large subunit 841941..842201 Saccharomyces cerevisiae 855330 NP_014014.1 CDS DSS1 NC_001145.2 842435 845344 R Dss1p; 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs complement(842435..845344) Saccharomyces cerevisiae 855331 NP_014015.1 CDS HSH155 NC_001145.2 845570 848485 D U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155; Hsh155p 845570..848485 Saccharomyces cerevisiae 855332 NP_014016.1 CDS ABZ2 NC_001145.2 848684 849808 D Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis; Abz2p 848684..849808 Saccharomyces cerevisiae 855333 NP_014017.1 CDS HAS1 NC_001145.2 850073 851590 R Has1p; ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles complement(850073..851590) Saccharomyces cerevisiae 855335 NP_014019.1 CDS YMR291W NC_001145.2 852629 854389 D Ymr291wp; Putative kinase of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR291W is not an essential gene 852629..854389 Saccharomyces cerevisiae 855336 NP_014020.1 CDS GOT1 NC_001145.2 854794 855292 D Got1p; Evolutionarily conserved non-essential protein present in early Golgi cisternae that may be involved in ER-Golgi transport at a step after vesicle tethering to Golgi membranes, exhibits membrane topology similar to that of Sft2p join(854794..854815,854898..855292) Saccharomyces cerevisiae 855337 NP_014021.1 CDS LRC6 NC_001145.2 855398 856792 R Putative protein similar to bacterial glutamyl-tRNA amidotransferases; non-tagged protein is detected in purified mitochondria; null mutant displays decreased frequency of mitochondrial genome loss (petite formation); Lrc6p complement(855398..856792) Saccharomyces cerevisiae 855338 NP_014022.1 CDS JNM1 NC_001145.2 856965 858086 D Jnm1p; Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B 856965..858086 Saccharomyces cerevisiae 855339 NP_014024.1 CDS YMR295C NC_001145.2 858296 858889 R Ymr295cp; Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene complement(858296..858889) Saccharomyces cerevisiae 855341 NP_014025.1 CDS LCB1 NC_001145.2 859214 860890 R Lcb1p; Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine complement(859214..860890) Saccharomyces cerevisiae 855342 NP_014026.1 CDS PRC1 NC_001145.2 861921 863519 D Vacuolar carboxypeptidase Y (proteinase C), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family; Prc1p 861921..863519 Saccharomyces cerevisiae 855343 NP_014027.1 CDS LIP1 NC_001145.2 863818 864270 D Lip1p; Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis 863818..864270 Saccharomyces cerevisiae 855344 NP_014028.1 CDS DYN3 NC_001145.2 864411 865349 R Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration; Dyn3p complement(864411..865349) Saccharomyces cerevisiae 855345 NP_014029.1 CDS ADE4 NC_001145.2 865558 867090 R Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; Ade4p complement(865558..867090) Saccharomyces cerevisiae 855346 NP_014030.1 CDS ATM1 NC_001145.2 867554 869626 R Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; Atm1p complement(867554..869626) Saccharomyces cerevisiae 855347 NP_014031.1 CDS YME2 NC_001145.2 870072 872624 R Yme2p; Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases complement(870072..872624) Saccharomyces cerevisiae 855348 NP_014032.1 CDS ADH2 NC_001145.2 873290 874336 R Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1; Adh2p complement(873290..874336) Saccharomyces cerevisiae 855349 NP_014033.1 CDS UBP15 NC_001145.2 874986 878678 D Ubp15p; Ubiquitin-specific protease that may play a role in ubiquitin precursor processing 874986..878678 Saccharomyces cerevisiae 855350 NP_014035.1 CDS SCW10 NC_001145.2 879062 880231 R Scw10p; Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p complement(879062..880231) Saccharomyces cerevisiae 855352 NP_014036.1 CDS FKS3 NC_001145.2 881158 886515 D Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fks3p 881158..886515 Saccharomyces cerevisiae 855353 NP_014038.1 CDS GAS1 NC_001145.2 887002 888681 D Gas1p; Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor 887002..888681 Saccharomyces cerevisiae 855355 NP_014039.1 CDS PSE1 NC_001145.2 888951 892220 R Pse1p; Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p complement(888951..892220) Saccharomyces cerevisiae 855356 NP_014040.1 CDS NIP1 NC_001145.2 892987 895425 R Subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection; Nip1p complement(892987..895425) Saccharomyces cerevisiae 855357 NP_014041.1 CDS YMR310C NC_001145.2 895715 896668 R Ymr310cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene complement(895715..896668) Saccharomyces cerevisiae 855358 NP_014042.1 CDS GLC8 NC_001145.2 896913 897602 R Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; Glc8p complement(896913..897602) Saccharomyces cerevisiae 855359 NP_014043.1 CDS ELP6 NC_001145.2 898404 899225 D Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity; Elp6p 898404..899225 Saccharomyces cerevisiae 855360 NP_014044.1 CDS TGL3 NC_001145.2 899378 901306 R Triacylglycerol lipase of the lipid particle, responsible for all the TAG lipase activity of the lipid particle; contains the consensus sequence motif GXSXG, which is found in lipolytic enzymes; Tgl3p complement(899378..901306) Saccharomyces cerevisiae 855361 NP_014045.1 CDS PRE5 NC_001145.2 901708 902412 D Pre5p; 20S proteasome alpha-type subunit 901708..902412 Saccharomyces cerevisiae 855362 NP_014046.1 CDS YMR315W NC_001145.2 902799 903848 D Ymr315wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YMR315W is not an essential gene 902799..903848 Saccharomyces cerevisiae 855363 NP_878150.1 CDS YMR315W-A NC_001145.2 904285 904392 D Ymr315w-ap; Putative protein of unknown function 904285..904392 Saccharomyces cerevisiae 1466508 NP_014047.1 CDS DIA1 NC_001145.2 904823 905833 D Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Dia1p 904823..905833 Saccharomyces cerevisiae 855364 NP_014050.1 CDS YMR317W NC_001145.2 907362 910784 D Ymr317wp; Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene 907362..910784 Saccharomyces cerevisiae 855366 NP_014051.1 CDS ADH6 NC_001145.2 911059 912141 R NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; Adh6p complement(911059..912141) Saccharomyces cerevisiae 855368 NP_014052.1 CDS FET4 NC_001145.2 912878 914536 R Low-affinity Fe(II) transporter of the plasma membrane; Fet4p complement(912878..914536) Saccharomyces cerevisiae 855369 NP_014054.1 CDS YMR321C NC_001145.2 917577 917894 R Ymr321cp; Putative protein of unknown function complement(917577..917894) Saccharomyces cerevisiae 855371 NP_014055.1 CDS SNO4 NC_001145.2 918364 919077 R Sno4p; Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Hsp33p; member of the DJ-1/ThiJ/PfpI superfamily; may have a role in pyridoxine metabolism complement(918364..919077) Saccharomyces cerevisiae 855372 NP_014056.1 CDS ERR3 NC_001145.2 920086 921399 D Protein of unknown function, has similarity to enolases; Err3p 920086..921399 Saccharomyces cerevisiae 855373 NP_014058.1 CDS PAU19 NC_001145.2 922639 923013 D Pau19p 922639..923013 Saccharomyces cerevisiae 855375 NP_014060.1 CDS YRF1-6 NC_001146.6 371 6098 R Yrf1-6p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p complement(join(371..5931,6080..6098)) Saccharomyces cerevisiae 855377 NP_014063.1 CDS COS1 NC_001146.6 8330 9475 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos1p 8330..9475 Saccharomyces cerevisiae 855380 NP_014064.1 CDS DDI3 NC_001146.6 11452 12129 D Ddi3p 11452..12129 Saccharomyces cerevisiae 855381 NP_014065.1 CDS SNO2 NC_001146.6 12208 12876 R Sno2p; Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin complement(12208..12876) Saccharomyces cerevisiae 855382 NP_014066.1 CDS SNZ2 NC_001146.6 13267 14163 D Snz2p; Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p 13267..14163 Saccharomyces cerevisiae 855383 NP_014067.1 CDS THI12 NC_001146.6 14832 15854 D Thi12p; Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 14832..15854 Saccharomyces cerevisiae 855384 NP_014068.1 CDS AAD14 NC_001146.6 16118 17248 R Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; Aad14p complement(16118..17248) Saccharomyces cerevisiae 855385 NP_014069.1 CDS RPD3 NC_001146.6 18001 19302 R Rpd3p; Histone deacetylase; regulates transcription and silencing complement(18001..19302) Saccharomyces cerevisiae 855386 NP_014070.1 CDS PEX6 NC_001146.6 19541 22633 R Pex6p; AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol complement(19541..22633) Saccharomyces cerevisiae 855387 NP_014071.1 CDS MDJ2 NC_001146.6 22834 23274 R Mdj2p; Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain complement(22834..23274) Saccharomyces cerevisiae 855388 NP_014072.1 CDS EGT2 NC_001146.6 24047 27172 D Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner; Egt2p 24047..27172 Saccharomyces cerevisiae 855389 NP_014073.1 CDS PFA3 NC_001146.6 27336 28346 R Pfa3p; Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions complement(27336..28346) Saccharomyces cerevisiae 855390 NP_014074.1 CDS FIG4 NC_001146.6 28738 31377 R Polyphosphatidylinositol phosphatase involved in the metabolism of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2); required for efficient mating; physically associates with and regulated by Vac14p; contains a SAC1-like domain; Fig4p complement(28738..31377) Saccharomyces cerevisiae 855392 NP_014076.1 CDS LEM3 NC_001146.6 31943 33187 D Membrane protein of the plasma membrane and ER, involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; Lem3p 31943..33187 Saccharomyces cerevisiae 855393 NP_014077.1 CDS KRE1 NC_001146.6 33293 34234 R Kre1p; Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor complement(33293..34234) Saccharomyces cerevisiae 855394 NP_014078.1 CDS VNX1 NC_001146.6 34695 37421 D Vnx1p; Low affinity vacuolar membrane localized monovalent cation/H+ antiporter; member of the calcium exchanger (CAX) family; potential Cdc28p substrate 34695..37421 Saccharomyces cerevisiae 855395 NP_014079.1 CDS YNL320W NC_001146.6 37699 38553 D Ynl320wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 37699..38553 Saccharomyces cerevisiae 855396 NP_014081.1 CDS HXT14 NC_001146.6 38706 40328 R Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Hxt14p complement(38706..40328) Saccharomyces cerevisiae 855398 NP_014082.1 CDS PFS2 NC_001146.6 40618 42015 D Pfs2p; Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex 40618..42015 Saccharomyces cerevisiae 855399 NP_014083.2 CDS PHA2 NC_001146.6 42070 43074 R Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway; Pha2p complement(42070..43074) Saccharomyces cerevisiae 855400 NP_014084.1 CDS ATP11 NC_001146.6 43323 44279 R Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; Atp11p complement(43323..44279) Saccharomyces cerevisiae 855401 NP_014085.1 CDS DAL82 NC_001146.6 44446 45213 D Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain; Dal82p 44446..45213 Saccharomyces cerevisiae 855402 NP_014086.1 CDS YNL313C NC_001146.6 45307 48021 R Ynl313cp; Putative protein of unknown function; identified as interacting with Kar2p, Grs1p, and Tub3p in high-throughput TAP-tagging experiments; YNL313C is an essential gene complement(45307..48021) Saccharomyces cerevisiae 855403 NP_014087.1 CDS RFA2 NC_001146.6 48286 49215 D Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; Rfa2p join(48286..48292,48401..49215) Saccharomyces cerevisiae 855404 NP_014088.1 CDS SKP2 NC_001146.6 49396 51687 R Skp2p; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; putative F-box protein; analysis of integrated high-throughput datasets predicts involvement in ubiquitin-dependent protein catabolism complement(49396..51687) Saccharomyces cerevisiae 855405 NP_014089.2 CDS ZIM17 NC_001146.6 51906 52430 R Zim17p; Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix complement(51906..52430) Saccharomyces cerevisiae 855406 NP_014090.2 CDS STB1 NC_001146.6 52661 53923 D Stb1p; Protein with a role in regulation of MBF-specific transcription at Start, phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p and binding is required for Stb1p function; expression is cell-cycle regulated 52661..53923 Saccharomyces cerevisiae 855407 NP_014091.1 CDS KRI1 NC_001146.6 54121 55896 R Kri1p; Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally interacts with Krr1p complement(54121..55896) Saccharomyces cerevisiae 855408 NP_014092.1 CDS MCK1 NC_001146.6 56446 57573 R Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry into meiosis; related to mammalian glycogen synthase kinases of the GSK-3 family; Mck1p complement(56446..57573) Saccharomyces cerevisiae 855409 NP_014093.1 CDS MRPS18 NC_001146.6 58155 58808 D Mrps18p; Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins 58155..58808 Saccharomyces cerevisiae 855410 NP_014094.1 CDS YNL305C NC_001146.6 58898 59791 R Ynl305cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL305C is not an essential gene complement(58898..59791) Saccharomyces cerevisiae 855411 NP_014095.2 CDS YPT11 NC_001146.6 60297 61550 D Ypt11p; Rab-type small GTPase that interacts with the C-terminal tail domain of Myo2p to mediate distribution of mitochondria to daughter cells 60297..61550 Saccharomyces cerevisiae 855412 NP_014097.1 CDS RPS19B NC_001146.6 61958 62943 R Rps19bp; Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Ap complement(join(61958..62372,62924..62943)) Saccharomyces cerevisiae 855414 NP_014098.1 CDS RPL18B NC_001146.6 63570 64562 R Rpl18bp; Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein complement(join(63570..64018,64451..64562)) Saccharomyces cerevisiae 855415 NP_014099.1 CDS YNL300W NC_001146.6 65744 66052 D Ynl300wp; Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid 65744..66052 Saccharomyces cerevisiae 855416 NP_014100.2 CDS TRF5 NC_001146.6 66517 68445 D Trf5p; Poly (A) polymerase involved in nuclear RNA quality control based on: homology with Trf4p, genetic interactions with TRF4 mutants, physical interaction with Mtr4p (TRAMP subunit), and by direct assay; disputed role as a sigma DNA polymerase 66517..68445 Saccharomyces cerevisiae 855417 NP_014101.1 CDS CLA4 NC_001146.6 68915 71443 D Cla4p; Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p 68915..71443 Saccharomyces cerevisiae 855418 NP_014102.1 CDS MON2 NC_001146.6 71673 76583 R Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins; Mon2p complement(71673..76583) Saccharomyces cerevisiae 855420 NP_014104.1 CDS YNL295W NC_001146.6 76946 78520 D Ynl295wp; Putative protein of unknown function 76946..78520 Saccharomyces cerevisiae 855421 NP_014105.1 CDS RIM21 NC_001146.6 78659 80260 R Rim21p; Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH complement(78659..80260) Saccharomyces cerevisiae 855422 NP_014106.1 CDS MSB3 NC_001146.6 80640 82541 D Msb3p; GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth 80640..82541 Saccharomyces cerevisiae 855423 NP_014107.1 CDS PUS4 NC_001146.6 82806 84017 D Pus4p; Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) 82806..84017 Saccharomyces cerevisiae 855424 NP_014108.1 CDS MID1 NC_001146.6 84169 85815 R N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer; Mid1p complement(84169..85815) Saccharomyces cerevisiae 855425 NP_014109.1 CDS RFC3 NC_001146.6 86218 87240 D Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc3p 86218..87240 Saccharomyces cerevisiae 855426 NP_014110.1 CDS PCL1 NC_001146.6 87897 88736 D Pho85 cyclin of the Pcl1,2-like subfamily, involved in entry into the mitotic cell cycle and regulation of morphogenesis, localizes to sites of polarized cell growth; Pcl1p 87897..88736 Saccharomyces cerevisiae 855427 NP_014111.1 CDS CAF40 NC_001146.6 90303 91424 D Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p; Caf40p 90303..91424 Saccharomyces cerevisiae 855428 NP_014112.1 CDS SEC21 NC_001146.6 91994 94801 D Sec21p; Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo 91994..94801 Saccharomyces cerevisiae 855429 NP_014113.1 CDS CUS2 NC_001146.6 95223 96080 D Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs); Cus2p 95223..96080 Saccharomyces cerevisiae 855430 NP_058178.1 CDS YNL284C-B NC_001146.6 96964 102232 R Ynl284c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(96964..100926,100928..102232)) Saccharomyces cerevisiae 855433 NP_058179.1 CDS YNL284C-A NC_001146.6 100910 102232 R Ynl284c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(100910..102232) Saccharomyces cerevisiae 855434 NP_014115.1 CDS MRPL10 NC_001146.6 103134 104102 R Mrpl10p; Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels complement(103134..104102) Saccharomyces cerevisiae 855436 NP_014116.1 CDS WSC2 NC_001146.6 105184 106695 R Wsc2p; Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response complement(105184..106695) Saccharomyces cerevisiae 855438 NP_014117.1 CDS POP3 NC_001146.6 107687 108274 D Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; Pop3p 107687..108274 Saccharomyces cerevisiae 855439 NP_014118.1 CDS HCH1 NC_001146.6 108467 108928 D Heat shock protein regulator that binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; Hch1p 108467..108928 Saccharomyces cerevisiae 855440 NP_014119.1 CDS ERG24 NC_001146.6 109095 110411 R Erg24p; C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions complement(109095..110411) Saccharomyces cerevisiae 855441 NP_014120.1 CDS PRM1 NC_001146.6 110917 112902 D Prm1p; Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p 110917..112902 Saccharomyces cerevisiae 855442 NP_014121.1 CDS CAF120 NC_001146.6 113271 116453 D Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation; Caf120p 113271..116453 Saccharomyces cerevisiae 855443 NP_878151.1 CDS YNL277W-A NC_001146.6 116679 116867 D Ynl277w-ap; Putative protein of unknown function 116679..116867 Saccharomyces cerevisiae 1466509 NP_014122.1 CDS MET2 NC_001146.6 117349 118809 D Met2p; L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway 117349..118809 Saccharomyces cerevisiae 855444 NP_014124.1 CDS BOR1 NC_001146.6 119268 120998 D Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1; Bor1p 119268..120998 Saccharomyces cerevisiae 855446 NP_014125.1 CDS GOR1 NC_001146.6 121118 122170 R Gor1p; Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(121118..122170) Saccharomyces cerevisiae 855447 NP_014126.1 CDS TOF1 NC_001146.6 122883 126599 D Tof1p; Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase 122883..126599 Saccharomyces cerevisiae 855448 NP_014127.1 CDS SEC2 NC_001146.6 126805 129084 R Sec2p; Guanyl-nucleotide exchange factor for the small G-protein Sec4p, located on cytoplasmic vesicles; essential for post-Golgi vesicle transport complement(126805..129084) Saccharomyces cerevisiae 855449 NP_014128.1 CDS BNI1 NC_001146.6 129523 135384 R Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1; Bni1p complement(129523..135384) Saccharomyces cerevisiae 855450 NP_014129.1 CDS ALP1 NC_001146.6 135941 137662 R Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; Alp1p complement(135941..137662) Saccharomyces cerevisiae 855451 NP_014130.1 CDS BSC4 NC_001146.6 137700 138095 D Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; Bsc4p 137700..138095 Saccharomyces cerevisiae 855452 NP_014131.1 CDS LYP1 NC_001146.6 138551 140386 D Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids; Lyp1p 138551..140386 Saccharomyces cerevisiae 855453 NP_014132.1 CDS PIK1 NC_001146.6 140879 144079 D Pik1p; Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokineses through the actin cytoskeleton 140879..144079 Saccharomyces cerevisiae 855454 NP_014134.1 CDS IST1 NC_001146.6 144281 145282 R Putative translation initiation factor, as suggested by computational analysis of large-scale protein-protein interaction data; Ist1p complement(join(144281..145150,145256..145282)) Saccharomyces cerevisiae 855456 NP_014135.1 CDS PDR17 NC_001146.6 145564 146616 R Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition; Pdr17p complement(145564..146616) Saccharomyces cerevisiae 855457 NP_014136.1 CDS YIF1 NC_001146.6 146897 147841 R Yif1p; Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles complement(146897..147841) Saccharomyces cerevisiae 855458 NP_014137.1 CDS POL2 NC_001146.6 148213 154881 D Pol2p; Catalytic subunit of DNA polymerase (II) epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair 148213..154881 Saccharomyces cerevisiae 855459 NP_014138.1 CDS ORC5 NC_001146.6 155101 156540 D Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; Orc5p 155101..156540 Saccharomyces cerevisiae 855460 NP_014139.1 CDS YNL260C NC_001146.6 156860 157456 R Ynl260cp; Putative protein of unknown function with similarity to a human protein overexpressed in oral cancers; localizes to the nucleus and cytoplasm; YNL260C is an essential gene complement(156860..157456) Saccharomyces cerevisiae 855461 NP_014140.1 CDS ATX1 NC_001146.6 157645 157866 R Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; Atx1p complement(157645..157866) Saccharomyces cerevisiae 855462 NP_014141.1 CDS DSL1 NC_001146.6 158110 160374 R Peripheral membrane protein required for Golgi-to-ER retrograde traffic; component of the ER target site that interacts with coatomer, the major component of the COPI vesicle protein coat; also interacts with Cin5p and Sec39p; Dsl1p complement(158110..160374) Saccharomyces cerevisiae 855463 NP_014142.1 CDS SIP3 NC_001146.6 160632 164321 R Sip3p; Protein that activates transcription through interaction with DNA-bound Snf1p, C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate complement(160632..164321) Saccharomyces cerevisiae 855464 NP_014143.2 CDS FOL1 NC_001146.6 164624 167098 D Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities; Fol1p 164624..167098 Saccharomyces cerevisiae 855465 NP_014144.1 CDS GIS2 NC_001146.6 167330 167791 R Protein with seven cysteine-rich CCHC zinc-finger motifs, similar to human CNBP, proposed to be involved in the RAS/cAMP signaling pathway; Gis2p complement(167330..167791) Saccharomyces cerevisiae 855466 NP_014145.1 CDS YNL254C NC_001146.6 168044 169249 R Ynl254cp complement(168044..169249) Saccharomyces cerevisiae 855467 NP_014146.1 CDS TEX1 NC_001146.6 170019 171287 D Tex1p; Protein involved in mRNA export, component of the transcription export (TREX) complex 170019..171287 Saccharomyces cerevisiae 855468 NP_014147.1 CDS MRPL17 NC_001146.6 171442 172287 R Mrpl17p; Mitochondrial ribosomal protein of the large subunit complement(171442..172287) Saccharomyces cerevisiae 855469 NP_014148.1 CDS NRD1 NC_001146.6 172589 174316 R RNA-binding protein that interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), required for transcription termination and 3' end maturation of nonpolyadenylated RNAs; Nrd1p complement(172589..174316) Saccharomyces cerevisiae 855470 NP_014149.1 CDS RAD50 NC_001146.6 175411 179349 D Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; Rad50p 175411..179349 Saccharomyces cerevisiae 855471 NP_014150.1 CDS MPA43 NC_001146.6 179395 181023 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Mpa43p complement(179395..181023) Saccharomyces cerevisiae 855472 NP_014151.1 CDS RPA49 NC_001146.6 181362 182609 R Rpa49p; RNA polymerase I subunit A49 complement(181362..182609) Saccharomyces cerevisiae 855473 NP_014152.1 CDS YNL247W NC_001146.6 182876 185179 D Ynl247wp; Essential protein of unknown function; may interact with ribosomes, based on co-purification experiments 182876..185179 Saccharomyces cerevisiae 855474 NP_014153.1 CDS VPS75 NC_001146.6 185461 186350 D Vps75p; NAP family histone chaperone; binds to histone H3-H4 tetramers and to histone acetyltransferase RTT109; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting join(185461..185492,185588..186350) Saccharomyces cerevisiae 855475 NP_014154.1 CDS CWC25 NC_001146.6 186347 186886 R Cwc25p; Component of a complex containing Cef1p, involved in pre-mRNA splicing; has similarity to S. pombe Cwf25p complement(186347..186886) Saccharomyces cerevisiae 855476 NP_014155.1 CDS SUI1 NC_001146.6 187171 187497 R Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase; Sui1p complement(187171..187497) Saccharomyces cerevisiae 855477 NP_014156.1 CDS SLA2 NC_001146.6 188052 190958 D Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo; Sla2p 188052..190958 Saccharomyces cerevisiae 855478 NP_014157.1 CDS ATG2 NC_001146.6 191325 196103 D Peripheral membrane protein required for the formation of cytosolic sequestering vesicles involved in vacuolar import through both the Cvt pathway and autophagy; interacts with Atg9p and is necessary for its trafficking; Atg2p 191325..196103 Saccharomyces cerevisiae 855479 NP_014158.1 CDS ZWF1 NC_001146.6 196427 197944 R Zwf1p; Glucose-6-phosphate dehydrogenase (G6PD), catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia complement(196427..197944) Saccharomyces cerevisiae 855480 NP_014159.1 CDS NAR1 NC_001146.6 198503 199978 R Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf; Nar1p complement(198503..199978) Saccharomyces cerevisiae 855481 NP_014160.2 CDS LAP3 NC_001146.6 200570 201934 D Lap3p; Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH 200570..201934 Saccharomyces cerevisiae 855482 NP_014161.1 CDS KEX2 NC_001146.6 202429 204873 D Subtilisin-like protease (proprotein convertase), a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway; Kex2p 202429..204873 Saccharomyces cerevisiae 855483 NP_014162.1 CDS YTP1 NC_001146.6 205189 206568 D Ytp1p; Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins 205189..206568 Saccharomyces cerevisiae 855484 NP_014163.1 CDS SIN4 NC_001146.6 206931 209855 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription; Sin4p 206931..209855 Saccharomyces cerevisiae 855485 NP_014165.1 CDS YNL234W NC_001146.6 210234 211514 D Ynl234wp; Similar to globins and has a functional heme-binding domain; involved in glucose signaling or metabolism; regulated by Rgt1p 210234..211514 Saccharomyces cerevisiae 855487 NP_014166.1 CDS BNI4 NC_001146.6 211923 214601 D Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; Bni4p 211923..214601 Saccharomyces cerevisiae 855488 NP_014167.1 CDS CSL4 NC_001146.6 214924 215802 D Subunit of the exosome, which is an essential complex present in both nucleus and cytoplasm that mediates RNA processing and degradation; Csl4p 214924..215802 Saccharomyces cerevisiae 855489 NP_014168.1 CDS PDR16 NC_001146.6 215988 217043 R Phosphatidylinositol transfer protein (PITP) controlled by the multiple drug resistance regulator Pdr1p, localizes to lipid particles and microsomes, controls levels of various lipids, may regulate lipid synthesis, homologous to Pdr17p; Pdr16p complement(215988..217043) Saccharomyces cerevisiae 855490 NP_014169.1 CDS ELA1 NC_001146.6 217524 218663 R Elongin A, F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degredation of the RNA Polymerase II subunit RPO21; subunit of the Elongin-Cullin-Socs (ECS) ligase complex; Ela1p complement(217524..218663) Saccharomyces cerevisiae 855491 NP_014170.1 CDS URE2 NC_001146.6 219138 220202 R Ure2p; Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; altered form of Ure2p creates [URE3] prion complement(219138..220202) Saccharomyces cerevisiae 855492 NP_014172.1 CDS JJJ1 NC_001146.6 220660 222432 R Jjj1p; Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis complement(220660..222432) Saccharomyces cerevisiae 855495 NP_014174.1 CDS CNM67 NC_001146.6 222725 224470 R Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; Cnm67p complement(222725..224470) Saccharomyces cerevisiae 855496 NP_014175.1 CDS SQS1 NC_001146.6 224797 227100 R Sqs1p; Protein of unknown function; overexpression antagonizes the suppression of splicing defects by spp382 mutants; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus complement(224797..227100) Saccharomyces cerevisiae 855497 NP_014176.2 CDS ATG4 NC_001146.6 227371 228855 D Cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation; mediates attachment of autophagosomes to microtubules through interactions with Tub1p and Tub2p; Atg4p 227371..228855 Saccharomyces cerevisiae 855498 NP_014177.1 CDS SSU72 NC_001146.6 229095 229715 D Transcription/RNA-processing factor that associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 of the RNA polymerase II C-terminal domain; affects start site selection in vivo; Ssu72p 229095..229715 Saccharomyces cerevisiae 855499 NP_014178.1 CDS POP1 NC_001146.6 231069 233696 R Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in RNase P; Pop1p complement(231069..233696) Saccharomyces cerevisiae 855500 NP_014179.1 CDS ADE12 NC_001146.6 234414 235715 D Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence; Ade12p 234414..235715 Saccharomyces cerevisiae 855501 NP_014180.1 CDS ALG9 NC_001146.6 235997 237664 R Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation; Alg9p complement(235997..237664) Saccharomyces cerevisiae 855502 NP_014181.1 CDS MGS1 NC_001146.6 238239 240002 D Protein with DNA-dependent ATPase and ssDNA annealing activities involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); Mgs1p 238239..240002 Saccharomyces cerevisiae 855503 NP_014182.1 CDS YNL217W NC_001146.6 240332 241312 D Ynl217wp 240332..241312 Saccharomyces cerevisiae 855504 NP_014183.1 CDS RAP1 NC_001146.6 241690 244173 D Rap1p; DNA-binding protein involved in either activation or repression of transcription, depending on binding site context; also binds telomere sequences and plays a role in telomeric position effect (silencing) and telomere structure 241690..244173 Saccharomyces cerevisiae 855505 NP_014184.1 CDS IES2 NC_001146.6 244469 245431 D Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; Ies2p 244469..245431 Saccharomyces cerevisiae 855506 NP_014185.1 CDS PEX17 NC_001146.6 245618 246217 D Pex17p; Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis 245618..246217 Saccharomyces cerevisiae 855507 NP_014186.1 CDS YNL213C NC_001146.6 246461 247105 R Ynl213cp; Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(246461..247105) Saccharomyces cerevisiae 855508 NP_014187.1 CDS VID27 NC_001146.6 247462 249810 D Vid27p; Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth 247462..249810 Saccharomyces cerevisiae 855509 NP_014188.1 CDS YNL211C NC_001146.6 250056 250316 R Ynl211cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene complement(250056..250316) Saccharomyces cerevisiae 855510 NP_014189.1 CDS MER1 NC_001146.6 250932 251744 D Protein with RNA-binding motifs required for meiosis-specific mRNA splicing; required for chromosome pairing and meiotic recombination; Mer1p 250932..251744 Saccharomyces cerevisiae 855511 NP_014190.1 CDS SSB2 NC_001146.6 252060 253901 D Ssb2p; Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; homolog of SSB1 252060..253901 Saccharomyces cerevisiae 855512 NP_014191.2 CDS YNL208W NC_001146.6 254419 255018 D Ynl208wp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi 254419..255018 Saccharomyces cerevisiae 855513 NP_014192.1 CDS RIO2 NC_001146.6 255354 256631 D Rio2p; Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p 255354..256631 Saccharomyces cerevisiae 855514 NP_014193.1 CDS RTT106 NC_001146.6 256789 258156 R Rtt106p; Protein with a role in regulation of Ty1 transposition complement(256789..258156) Saccharomyces cerevisiae 855515 NP_014195.1 CDS SPS18 NC_001146.6 258376 259278 R Sps18p; Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation complement(258376..259278) Saccharomyces cerevisiae 855517 NP_014197.2 CDS SPS19 NC_001146.6 259579 260457 D Sps19p; Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate 259579..260457 Saccharomyces cerevisiae 855518 NP_014198.1 CDS PSY2 NC_001146.6 260628 263204 R Putative subunit of an evolutionarily conserved protein phosphatase complex containing the catalytic subunit Pph3p and the regulatory subunit Psy4p; required for cisplatin and oxaliplatin resistance; localizes to nucleus; Psy2p complement(260628..263204) Saccharomyces cerevisiae 855520 NP_014199.1 CDS YNL200C NC_001146.6 263714 264454 R Ynl200cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(263714..264454) Saccharomyces cerevisiae 855521 NP_014200.1 CDS GCR2 NC_001146.6 264927 266531 R Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p; Gcr2p complement(264927..266531) Saccharomyces cerevisiae 855522 NP_014202.1 CDS WHI3 NC_001146.6 267609 269594 R Whi3p; RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start complement(267609..269594) Saccharomyces cerevisiae 855524 NP_014203.1 CDS SLZ1 NC_001146.6 270276 271172 R Sporulation-specific protein with a leucine zipper motif; Slz1p complement(270276..271172) Saccharomyces cerevisiae 855525 NP_014204.2 CDS YNL195C NC_001146.6 271524 272309 R Ynl195cp; Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(271524..272309) Saccharomyces cerevisiae 855526 NP_014205.1 CDS YNL194C NC_001146.6 272711 273616 R Ynl194cp; Integral membrane protein localized to eisosomes; sporulation and plasma membrane sphingolipid content are altered in mutants; has homologs SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS complement(272711..273616) Saccharomyces cerevisiae 855527 NP_014206.1 CDS YNL193W NC_001146.6 274369 276045 D Ynl193wp; Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis 274369..276045 Saccharomyces cerevisiae 855528 NP_014207.1 CDS CHS1 NC_001146.6 276503 279898 D Chs1p; Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor 276503..279898 Saccharomyces cerevisiae 855529 NP_014208.1 CDS DUG3 NC_001146.6 280433 281506 D Probable glutamine amidotransferase, forms a complex with Dug1p and Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); Dug3p 280433..281506 Saccharomyces cerevisiae 855530 NP_014209.1 CDS YNL190W NC_001146.6 282396 283010 D Ynl190wp; Cell wall protein of unknown function; proposed role as a hydrophilin induced by osmotic stress; contains a putative GPI-attachment site 282396..283010 Saccharomyces cerevisiae 855531 NP_014210.1 CDS SRP1 NC_001146.6 284261 285889 D Srp1p; Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation 284261..285889 Saccharomyces cerevisiae 855532 NP_014211.1 CDS KAR1 NC_001146.6 286309 287610 D Kar1p; Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p 286309..287610 Saccharomyces cerevisiae 855533 NP_014212.1 CDS YNL187W NC_001146.6 287996 289069 D Ynl187wp; Putative protein of unknown function; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; may interact with or be fuctionally redundant with Prp40p 287996..289069 Saccharomyces cerevisiae 855534 NP_014213.1 CDS UBP10 NC_001146.6 289500 291878 D Ubp10p; Ubiquitin-specific protease that deubiquitinates ubiquitin-protein moieties; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; primarily located in the nucleus 289500..291878 Saccharomyces cerevisiae 855535 NP_014214.1 CDS MRPL19 NC_001146.6 292195 292671 R Mrpl19p; Mitochondrial ribosomal protein of the large subunit complement(292195..292671) Saccharomyces cerevisiae 855536 NP_014216.1 CDS NPR1 NC_001146.6 293139 295511 R Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; Npr1p complement(293139..295511) Saccharomyces cerevisiae 855538 NP_014217.1 CDS IPI3 NC_001146.6 295962 297629 R Ipi3p; Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; highly conserved and contains WD40 motifs; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles complement(295962..297629) Saccharomyces cerevisiae 855539 NP_014218.1 CDS YNL181W NC_001146.6 298337 299560 D Ynl181wp; Putative oxidoreductase; required for cell viability 298337..299560 Saccharomyces cerevisiae 855540 NP_014219.1 CDS RHO5 NC_001146.6 299656 300651 R Rho5p; Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity complement(299656..300651) Saccharomyces cerevisiae 855541 NP_014221.1 CDS RPS3 NC_001146.6 302682 303404 D Rps3p; Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins 302682..303404 Saccharomyces cerevisiae 855543 NP_014222.1 CDS MRPL22 NC_001146.6 303688 304617 R Mrpl22p; Mitochondrial ribosomal protein of the large subunit complement(303688..304617) Saccharomyces cerevisiae 855544 NP_014223.1 CDS YNL176C NC_001146.6 305072 306982 R Ynl176cp; Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p complement(305072..306982) Saccharomyces cerevisiae 855545 NP_014224.1 CDS NOP13 NC_001146.6 307403 308614 R Protein of unknown function, localizes to the nucleolus and nucleoplasm; contains an RNA recognition motif (RRM) and has similarity to Nop12p, which is required for processing of pre-18S rRNA; Nop13p complement(307403..308614) Saccharomyces cerevisiae 855547 NP_014226.1 CDS MDG1 NC_001146.6 308959 310059 R Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; Mdg1p complement(308959..310059) Saccharomyces cerevisiae 855548 NP_014227.1 CDS APC1 NC_001146.6 310638 315884 D Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Apc1p 310638..315884 Saccharomyces cerevisiae 855549 NP_014230.1 CDS PSD1 NC_001146.6 316171 317673 R Psd1p; Phosphatidylserine decarboxylase of the mitochondrial inner membrane, converts phosphatidylserine to phosphatidylethanolamine complement(316171..317673) Saccharomyces cerevisiae 855552 NP_014231.1 CDS FMP41 NC_001146.6 318032 318811 R Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp41p complement(318032..318811) Saccharomyces cerevisiae 855553 NP_014232.1 CDS SKO1 NC_001146.6 319418 321361 R Sko1p; Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, forms a complex with Tup1p and Ssn6p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses complement(319418..321361) Saccharomyces cerevisiae 855554 NP_014233.1 CDS BNI5 NC_001146.6 322221 323567 R Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner; Bni5p complement(322221..323567) Saccharomyces cerevisiae 855555 NP_014234.1 CDS YNL165W NC_001146.6 323834 325054 D Ynl165wp; Putative protein of unknown function; YNL165W is not an essential gene 323834..325054 Saccharomyces cerevisiae 855556 NP_014235.1 CDS IBD2 NC_001146.6 325267 326322 R Ibd2p; Component of the BUB2-dependent spindle checkpoint pathway, interacts with Bfa1p and functions upstream of Bub2p and Bfa1p complement(325267..326322) Saccharomyces cerevisiae 855557 NP_014236.1 CDS RIA1 NC_001146.6 326743 330075 R Ria1p; Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p) complement(326743..330075) Saccharomyces cerevisiae 855558 NP_076906.1 CDS YNL162W-A NC_001146.6 330331 330549 D Ynl162w-ap; Putative protein of unknown function, identified by homology 330331..330549 Saccharomyces cerevisiae 855559 NP_014237.2 CDS RPL42A NC_001146.6 331324 332156 D Rpl42ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl42Bp and has similarity to rat L44 ribosomal protein join(331324..331327,331840..332156) Saccharomyces cerevisiae 855560 NP_014238.1 CDS CBK1 NC_001146.6 332599 334869 D Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation; Cbk1p 332599..334869 Saccharomyces cerevisiae 855561 NP_014239.1 CDS YGP1 NC_001146.6 336547 337611 D Ygp1p; Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p 336547..337611 Saccharomyces cerevisiae 855562 NP_014240.1 CDS ASI2 NC_001146.6 338480 339349 R Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; Asi2p complement(338480..339349) Saccharomyces cerevisiae 855563 NP_014241.1 CDS PGA1 NC_001146.6 339614 340210 D Pga1p; Essential protein required for maturation of Gas1p and Pho8p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; has synthetic genetic interations with secretory pathway genes 339614..340210 Saccharomyces cerevisiae 855564 NP_014242.1 CDS IGO1 NC_001146.6 340354 340860 D Igo1p 340354..340860 Saccharomyces cerevisiae 855565 NP_014243.1 CDS NSG2 NC_001146.6 341071 341970 R Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; Nsg2p complement(341071..341970) Saccharomyces cerevisiae 855566 NP_014244.1 CDS YNL155W NC_001146.6 342518 343342 D Ynl155wp; Putative protein of unknown function, contains DHHC domain, also predicted to have thiol-disulfide oxidoreductase active site 342518..343342 Saccharomyces cerevisiae 855567 NP_014245.1 CDS YCK2 NC_001146.6 343638 345278 R Yck2p; Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck1p complement(343638..345278) Saccharomyces cerevisiae 855568 NP_014246.1 CDS GIM3 NC_001146.6 345671 346060 R Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it; Gim3p complement(345671..346060) Saccharomyces cerevisiae 855569 NP_014247.1 CDS YNL152W NC_001146.6 346315 347544 D Ynl152wp; Putative protein of unknown function; cytoplasmically localized protein proposed to function in phospholipid binding; YBL107C is an essential gene 346315..347544 Saccharomyces cerevisiae 855570 NP_014248.1 CDS RPC31 NC_001146.6 347768 348523 R Rpc31p; RNA polymerase III subunit C31; contains HMG-like C-terminal domain complement(347768..348523) Saccharomyces cerevisiae 855571 NP_014250.1 CDS PGA2 NC_001146.6 349369 349758 R Pga2p; Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect complement(349369..349758) Saccharomyces cerevisiae 855573 NP_014251.1 CDS ALF1 NC_001146.6 349909 350673 R Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance; Alf1p complement(349909..350673) Saccharomyces cerevisiae 855574 NP_014252.2 CDS LSM7 NC_001146.6 350942 351385 D Lsm7p; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA join(350942..350959,351056..351385) Saccharomyces cerevisiae 855575 NP_878152.1 CDS YNL146C-A NC_001146.6 351388 351582 R Ynl146c-ap; Putative protein of unknown function complement(351388..351582) Saccharomyces cerevisiae 1466510 NP_014253.1 CDS YNL146W NC_001146.6 351717 352019 D Ynl146wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene 351717..352019 Saccharomyces cerevisiae 855576 NP_014254.1 CDS MFA2 NC_001146.6 352416 352532 D Mfa2p; Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 352416..352532 Saccharomyces cerevisiae 855577 NP_014255.1 CDS YNL144C NC_001146.6 352822 355044 R Ynl144cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YNL144C is not an essential gene complement(352822..355044) Saccharomyces cerevisiae 855578 NP_014257.1 CDS MEP2 NC_001146.6 357455 358954 D Mep2p; Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation 357455..358954 Saccharomyces cerevisiae 855580 NP_014258.1 CDS AAH1 NC_001146.6 359598 360641 D Adenine deaminase (adenine aminohydrolase), involved in purine salvage and nitrogen catabolism; Aah1p 359598..360641 Saccharomyces cerevisiae 855581 NP_014260.1 CDS RLR1 NC_001146.6 360926 365719 R Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids; Rlr1p complement(360926..365719) Saccharomyces cerevisiae 855583 NP_878153.1 CDS YSF3 NC_001146.6 366035 366414 D Ysf3p; Component of the U2 snRNP, associated with the SF3b complex; conserved in Ashbya gossypii join(366035..366037,366160..366414) Saccharomyces cerevisiae 1466511 NP_014261.1 CDS SRV2 NC_001146.6 366743 368323 D Srv2p; CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis 366743..368323 Saccharomyces cerevisiae 855584 NP_014262.1 CDS NAM9 NC_001146.6 368597 370057 R Nam9p; Mitochondrial ribosomal component of the small subunit complement(368597..370057) Saccharomyces cerevisiae 855585 NP_014263.1 CDS EAF7 NC_001146.6 370370 371647 D Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A; Eaf7p 370370..371647 Saccharomyces cerevisiae 855586 NP_014264.1 CDS FPR1 NC_001146.6 371884 372228 R Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; Fpr1p complement(371884..372228) Saccharomyces cerevisiae 855587 NP_014265.1 CDS YNL134C NC_001146.6 372453 373583 R Ynl134cp; Putative protein of unknown function with similarity to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced by the DNA-damaging agent MMS complement(372453..373583) Saccharomyces cerevisiae 855588 NP_014266.1 CDS FYV6 NC_001146.6 374173 374694 R Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining; Fyv6p complement(374173..374694) Saccharomyces cerevisiae 855589 NP_014267.1 CDS KRE33 NC_001146.6 375323 378493 D Kre33p; Essential protein of unknown function; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance 375323..378493 Saccharomyces cerevisiae 855591 NP_014268.1 CDS TOM22 NC_001146.6 378769 379227 D Tom22p; Component of the TOM (translocase of outer membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between the TOM and TIM complexes 378769..379227 Saccharomyces cerevisiae 855592 NP_014269.2 CDS CPT1 NC_001146.6 379560 380833 R Cpt1p; Cholinephosphotransferase, required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription complement(join(379560..380691,380784..380833)) Saccharomyces cerevisiae 855593 NP_878154.1 CDS YNL130C-A NC_001146.6 381247 381393 R Ynl130c-ap; Putative protein of unknown function complement(381247..381393) Saccharomyces cerevisiae 1466512 NP_014270.1 CDS NRK1 NC_001146.6 381482 382204 D Nrk1p; Nicotinamide riboside kinase, catalyzes the synthesis of nicotinamide nucleotide (NMN) from nicotinamide riboside; involved in a salvage pathway for NAD+ biosynthesis 381482..382204 Saccharomyces cerevisiae 855594 NP_014271.1 CDS TEP1 NC_001146.6 382361 383665 D Tep1p; Homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 that has lipid phosphatase activity and is linked to the phosphatidylinositol signaling pathway; plays a role in normal sporulation 382361..383665 Saccharomyces cerevisiae 855595 NP_014272.1 CDS FAR11 NC_001146.6 383989 386850 D Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; Far11p 383989..386850 Saccharomyces cerevisiae 855596 NP_014273.1 CDS SPC98 NC_001146.6 387229 389769 D Spc98p; Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque 387229..389769 Saccharomyces cerevisiae 855597 NP_014274.1 CDS ESBP6 NC_001146.6 390148 392169 R Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane; Esbp6p complement(390148..392169) Saccharomyces cerevisiae 855598 NP_014275.1 CDS NAF1 NC_001146.6 392894 394372 D Protein required for the assembly of box H/ACA snoRNPs and for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; has similarity to Gar1p and other RNA-binding proteins; Naf1p 392894..394372 Saccharomyces cerevisiae 855599 NP_014276.1 CDS NMA111 NC_001146.6 394687 397680 D Protein of unknown function which may contribute to lipid homeostasis and/or apoptosis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases; Nma111p 394687..397680 Saccharomyces cerevisiae 855600 NP_014277.1 CDS YNL122C NC_001146.6 398025 398372 R Ynl122cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene complement(398025..398372) Saccharomyces cerevisiae 855601 NP_014278.1 CDS TOM70 NC_001146.6 398686 400539 R Tom70p; Component (70 kDa) of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins complement(398686..400539) Saccharomyces cerevisiae 855602 NP_014280.1 CDS NCS2 NC_001146.6 401042 402523 D Protein with a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; Ncs2p 401042..402523 Saccharomyces cerevisiae 855603 NP_014281.1 CDS DCP2 NC_001146.6 402654 405566 R Dcp2p; Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; member of the Nudix hydrolase family complement(402654..405566) Saccharomyces cerevisiae 855605 NP_014282.1 CDS MLS1 NC_001146.6 406360 408024 D Mls1p; Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium 406360..408024 Saccharomyces cerevisiae 855606 NP_014283.1 CDS DMA2 NC_001146.6 408343 409911 D Protein involved in regulating spindle position and orientation, functionally redundant with Dma1p; homolog of S. pombe Dma1 and H. sapiens Chfr; Dma2p 408343..409911 Saccharomyces cerevisiae 855607 NP_014284.1 CDS YNL115C NC_001146.6 410121 412055 R Ynl115cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene complement(410121..412055) Saccharomyces cerevisiae 855608 NP_014286.1 CDS RPC19 NC_001146.6 412773 413201 D Rpc19p; RNA polymerase subunit, common to RNA polymerases I and III 412773..413201 Saccharomyces cerevisiae 855609 NP_014287.1 CDS DBP2 NC_001146.6 413641 416283 D Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated mRNA decay and rRNA processing; Dbp2p join(413641..414913,415916..416283) Saccharomyces cerevisiae 855611 NP_014288.1 CDS CYB5 NC_001146.6 416942 417304 R Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; required for sterol C5-6 and fatty acid desaturation; Cyb5p complement(416942..417304) Saccharomyces cerevisiae 855612 NP_014289.1 CDS NOP15 NC_001146.6 417828 418490 R Nop15p; Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm complement(417828..418490) Saccharomyces cerevisiae 855613 NP_014291.1 CDS YNL108C NC_001146.6 419016 419828 R Ynl108cp; Putative protein of unknown function with similarity to Tfc7p and prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus complement(419016..419828) Saccharomyces cerevisiae 855615 NP_014292.1 CDS YAF9 NC_001146.6 420100 420780 D Yaf9p; Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain 420100..420780 Saccharomyces cerevisiae 855616 NP_014293.1 CDS INP52 NC_001146.6 420946 424497 R Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; Inp52p complement(420946..424497) Saccharomyces cerevisiae 855618 NP_014295.1 CDS LEU4 NC_001146.6 424897 426756 R Leu4p; Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway complement(424897..426756) Saccharomyces cerevisiae 855619 NP_014296.2 CDS MET4 NC_001146.6 427737 429755 D Met4p; Lecine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p 427737..429755 Saccharomyces cerevisiae 855620 NP_014297.1 CDS POL1 NC_001146.6 430089 434495 D Pol1p; Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis 430089..434495 Saccharomyces cerevisiae 855621 NP_014298.1 CDS AVT4 NC_001146.6 435001 437142 D Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt4p 435001..437142 Saccharomyces cerevisiae 855622 NP_014299.1 CDS AIM37 NC_001146.6 437615 438319 D Putative protein of unknown function; non-tagged protein is detected in purified mitochondria; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim37p 437615..438319 Saccharomyces cerevisiae 855623 NP_014300.1 CDS OCA1 NC_001146.6 438569 439285 R Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA; Oca1p complement(438569..439285) Saccharomyces cerevisiae 855624 NP_014301.1 CDS RAS2 NC_001146.6 439604 440572 R Ras2p; GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes complement(439604..440572) Saccharomyces cerevisiae 855625 NP_878155.1 CDS YNL097C-B NC_001146.6 440799 440921 R Ynl097c-bp; Putative protein of unknown function complement(440799..440921) Saccharomyces cerevisiae 1466513 NP_014302.1 CDS PHO23 NC_001146.6 441368 442360 R Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; C-terminus has similarity to human candidate tumor suppressor p33(ING1); Pho23p complement(441368..442360) Saccharomyces cerevisiae 855626 NP_014303.1 CDS RPS7B NC_001146.6 443400 444317 R Rps7bp; Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins complement(join(443400..443828,444174..444317)) Saccharomyces cerevisiae 855628 NP_014304.1 CDS YNL095C NC_001146.6 444914 446842 R Ynl095cp; Putative protein of unknown function predicted to contain a transmembrane domain; YNL095C is not an essential gene complement(444914..446842) Saccharomyces cerevisiae 855629 NP_014305.1 CDS APP1 NC_001146.6 447613 449376 D Protein of unknown function, interacts with Rvs161p and Rvs167p; computational analysis of protein-protein interactions in large-scale studies suggests a possible role in actin filament organization; App1p 447613..449376 Saccharomyces cerevisiae 855630 NP_014306.1 CDS YPT53 NC_001146.6 449870 450532 D Ypt53p; GTPase, similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis 449870..450532 Saccharomyces cerevisiae 855631 NP_014307.1 CDS YNL092W NC_001146.6 450873 452075 D Ynl092wp; Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene 450873..452075 Saccharomyces cerevisiae 855632 NP_014308.1 CDS NST1 NC_001146.6 452410 456132 D Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1; Nst1p 452410..456132 Saccharomyces cerevisiae 855633 NP_014309.1 CDS RHO2 NC_001146.6 456567 457145 D Rho2p; Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly 456567..457145 Saccharomyces cerevisiae 855634 NP_014311.1 CDS TOP2 NC_001146.6 457706 461992 D Top2p; Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis 457706..461992 Saccharomyces cerevisiae 855636 NP_014312.1 CDS TCB2 NC_001146.6 462413 465949 D Tcb2p; Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud 462413..465949 Saccharomyces cerevisiae 855637 NP_014313.1 CDS YNL086W NC_001146.6 466336 466644 D Ynl086wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes 466336..466644 Saccharomyces cerevisiae 855638 NP_014314.1 CDS MKT1 NC_001146.6 467133 469625 D Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO endonuclease; involved in propagation of M2 dsRNA satellite of L-A virus; contains a DTG signature typical of retroviral proteases; Mkt1p 467133..469625 Saccharomyces cerevisiae 855639 NP_014315.1 CDS END3 NC_001146.6 470055 471104 R End3p; EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p complement(470055..471104) Saccharomyces cerevisiae 855640 NP_014316.2 CDS SAL1 NC_001146.6 471379 473016 D Sal1p; Probable transporter, member of the Ca2+-binding subfamily of the mitochondrial carrier family, with two EF-hand motifs; Pet9p and Sal1p have an overlapping function critical for viability; polymorphic in different S. cerevisiae strains 471379..473016 Saccharomyces cerevisiae 855641 NP_014317.2 CDS PMS1 NC_001146.6 473392 476013 D Pms1p; ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL 473392..476013 Saccharomyces cerevisiae 855642 NP_014318.1 CDS SWS2 NC_001146.6 476189 476620 R Sws2p; Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency complement(476189..476620) Saccharomyces cerevisiae 855643 NP_014319.1 CDS EOS1 NC_001146.6 476933 478033 R Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene; Eos1p complement(476933..478033) Saccharomyces cerevisiae 855644 NP_014320.1 CDS TPM1 NC_001146.6 478567 479166 R Tpm1p; Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently complement(478567..479166) Saccharomyces cerevisiae 855645 NP_014321.1 CDS NIS1 NC_001146.6 479769 480992 D Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network; Nis1p 479769..480992 Saccharomyces cerevisiae 855646 NP_014322.1 CDS APJ1 NC_001146.6 481392 482978 D Putative chaperone of the HSP40 (DNAJ) family; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Apj1p 481392..482978 Saccharomyces cerevisiae 855647 NP_014323.1 CDS MKS1 NC_001146.6 483557 485311 D Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling; Mks1p 483557..485311 Saccharomyces cerevisiae 855648 NP_014324.1 CDS IMP4 NC_001146.6 485608 486480 D Imp4p; Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs 485608..486480 Saccharomyces cerevisiae 855649 NP_014325.1 CDS MLF3 NC_001146.6 486767 488125 R Serine-rich protein of unknown function; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide; Mlf3p complement(486767..488125) Saccharomyces cerevisiae 855650 NP_014326.1 CDS MSK1 NC_001146.6 488387 490117 D Msk1p; Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria 488387..490117 Saccharomyces cerevisiae 855651 NP_014327.1 CDS RNH201 NC_001146.6 490318 491241 D Rnh201p; Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis; cooperates with Rad27p nuclease 490318..491241 Saccharomyces cerevisiae 855652 NP_014328.1 CDS LAT1 NC_001146.6 491524 492972 D Lat1p; Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA 491524..492972 Saccharomyces cerevisiae 855653 NP_014329.1 CDS TOM7 NC_001146.6 493367 493549 D Tom7p; Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex 493367..493549 Saccharomyces cerevisiae 855654 NP_014330.1 CDS RPL16B NC_001146.6 493957 495002 R Rpl16bp; N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Ap, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p complement(join(493957..494525,494975..495002)) Saccharomyces cerevisiae 855655 NP_014331.1 CDS FKH2 NC_001146.6 495702 498290 R Forkhead family transcription factor with a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; Fkh2p complement(495702..498290) Saccharomyces cerevisiae 855656 NP_878156.1 CDS YNL067W-B NC_001146.6 499418 499558 D Ynl067w-bp; Putative protein of unknown function 499418..499558 Saccharomyces cerevisiae 1466514 NP_014332.1 CDS RPL9B NC_001146.6 499682 500257 D Rpl9bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins 499682..500257 Saccharomyces cerevisiae 855658 NP_014333.1 CDS SUN4 NC_001146.6 501516 502778 D Sun4p; Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family 501516..502778 Saccharomyces cerevisiae 855659 NP_014334.1 CDS AQR1 NC_001146.6 503725 505485 D Plasma membrane multidrug transporter of the major facilitator superfamily, confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; Aqr1p 503725..505485 Saccharomyces cerevisiae 855660 NP_014335.1 CDS YDJ1 NC_001146.6 505869 507098 R Ydj1p; Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family complement(505869..507098) Saccharomyces cerevisiae 855661 NP_014336.1 CDS MTQ1 NC_001146.6 507759 508703 D S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene; Mtq1p 507759..508703 Saccharomyces cerevisiae 855662 NP_014337.1 CDS GCD10 NC_001146.6 508777 510213 R Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression; Gcd10p complement(508777..510213) Saccharomyces cerevisiae 855663 NP_014338.1 CDS NOP2 NC_001146.6 510541 512397 D Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; Nop2p 510541..512397 Saccharomyces cerevisiae 855664 NP_014339.1 CDS ARP5 NC_001146.6 512669 514936 R Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; Arp5p complement(512669..514936) Saccharomyces cerevisiae 855665 NP_014340.1 CDS YNL058C NC_001146.6 515764 516714 R Ynl058cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene complement(515764..516714) Saccharomyces cerevisiae 855667 NP_014342.1 CDS OCA2 NC_001146.6 517250 517843 D Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene; Oca2p 517250..517843 Saccharomyces cerevisiae 855668 NP_014343.1 CDS POR1 NC_001146.6 517995 518846 R Por1p; Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated complement(517995..518846) Saccharomyces cerevisiae 855669 NP_058180.1 CDS YNL054W-A NC_001146.6 519457 520779 D Ynl054w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 519457..520779 Saccharomyces cerevisiae 855671 NP_014344.1 CDS VAC7 NC_001146.6 526087 529584 D Vac7p; Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock 526087..529584 Saccharomyces cerevisiae 855673 NP_014345.1 CDS MSG5 NC_001146.6 529943 531412 D Msg5p; Dual-specificity protein phosphatase required for maintenance of a low level of signaling through the cell integrity pathway; regulates and is regulated by Slt2p; dephosphorylates Fus3p; required for adaptive response to pheromone 529943..531412 Saccharomyces cerevisiae 855674 NP_014346.1 CDS COX5A NC_001146.6 531726 532187 D Subunit Va of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; Cox5ap 531726..532187 Saccharomyces cerevisiae 855675 NP_014347.1 CDS COG5 NC_001146.6 532660 533871 D Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog5p 532660..533871 Saccharomyces cerevisiae 855676 NP_014348.1 CDS YNL050C NC_001146.6 534080 534983 R Ynl050cp; Putative protein of unknown function; YNL050c is not an essential gene complement(join(534080..534875,534967..534983)) Saccharomyces cerevisiae 855677 NP_014349.1 CDS SFB2 NC_001146.6 535282 537912 R Sfb2p; Probable component of COPII coated vesicles that binds to Sec23p; similar to and functionally redundant with Sec24p, but expressed at low levels; involved in ER to Golgi transport and in autophagy complement(535282..537912) Saccharomyces cerevisiae 855678 NP_014350.1 CDS ALG11 NC_001146.6 538174 539820 D Alpha-1,2-mannosyltransferase, catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER; Alg11p 538174..539820 Saccharomyces cerevisiae 855679 NP_014351.1 CDS SLM2 NC_001146.6 539912 541882 R Slm2p; Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex complement(539912..541882) Saccharomyces cerevisiae 855680 NP_014352.1 CDS YNL046W NC_001146.6 542305 542823 D Ynl046wp; Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) 542305..542823 Saccharomyces cerevisiae 855681 NP_014353.1 CDS YNL045W NC_001146.6 542964 544979 D Ynl045wp; Leucyl aminopeptidase (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; role in vivo is not defined; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 542964..544979 Saccharomyces cerevisiae 855682 NP_014354.2 CDS YIP3 NC_001146.6 545269 545878 D Yip3p; Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p join(545269..545292,545372..545878) Saccharomyces cerevisiae 855683 NP_878157.1 CDS YNL042W-B NC_001146.6 547114 547371 D Ynl042w-bp; Putative protein of unknown function 547114..547371 Saccharomyces cerevisiae 1466515 NP_014356.1 CDS BOP3 NC_001146.6 548101 549291 D Protein of unknown function, potential Cdc28p substrate; overproduction suppresses a pam1 slv3 double null mutation and confers resistance to methylmercury; Bop3p 548101..549291 Saccharomyces cerevisiae 855686 NP_014357.1 CDS COG6 NC_001146.6 549469 551988 R Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog6p complement(549469..551988) Saccharomyces cerevisiae 855687 NP_014358.1 CDS YNL040W NC_001146.6 553381 554751 D Ynl040wp; Putative protein of unknown function with strong similarity to alanyl-tRNA synthases from Eubacteria; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene 553381..554751 Saccharomyces cerevisiae 855688 NP_014359.1 CDS BDP1 NC_001146.6 555049 556833 D Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs; Bdp1p 555049..556833 Saccharomyces cerevisiae 855689 NP_014360.2 CDS GPI15 NC_001146.6 557021 557784 D Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; Gpi15p join(557021..557611,557686..557784) Saccharomyces cerevisiae 855690 NP_014361.1 CDS IDH1 NC_001146.6 557921 559003 R Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; Idh1p complement(557921..559003) Saccharomyces cerevisiae 855691 NP_014362.1 CDS NCE103 NC_001146.6 559815 560480 D Nce103p; Carbonic anhydrase; poorly transcribed under aerobic conditions and at an undetectable level under anaerobic conditions; involved in non-classical protein export pathway 559815..560480 Saccharomyces cerevisiae 855692 NP_014363.1 CDS YNL035C NC_001146.6 568523 569692 R Ynl035cp; Putative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene complement(568523..569692) Saccharomyces cerevisiae 855695 NP_014364.1 CDS YNL034W NC_001146.6 570478 572316 D Ynl034wp; Putative protein of unknown function; YNL034W is not an essential gene 570478..572316 Saccharomyces cerevisiae 855697 NP_014365.1 CDS YNL033W NC_001146.6 573000 573854 D Ynl033wp; Putative protein of unknown function 573000..573854 Saccharomyces cerevisiae 855698 NP_014366.1 CDS SIW14 NC_001146.6 574507 575352 D Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm; Siw14p 574507..575352 Saccharomyces cerevisiae 855699 NP_014367.1 CDS HHT2 NC_001146.6 575641 576051 R One of two identical histone H3 proteins (see also HHT1); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation; Hht2p complement(575641..576051) Saccharomyces cerevisiae 855700 NP_014368.1 CDS HHF2 NC_001146.6 576728 577039 D One of two identical histone H4 proteins (see also HHF1); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity; Hhf2p 576728..577039 Saccharomyces cerevisiae 855701 NP_014369.1 CDS KTR5 NC_001146.6 577206 578774 R Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Ktr5p complement(577206..578774) Saccharomyces cerevisiae 855703 NP_014371.1 CDS CRZ1 NC_001146.6 579581 581617 D Transcription factor that activates transcription of genes involved in stress response; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; Crz1p 579581..581617 Saccharomyces cerevisiae 855704 NP_014372.1 CDS SAM50 NC_001146.6 581921 583375 D Sam50p; Essential component of the Sorting and Assembly Machinery (SAM or Tob complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 581921..583375 Saccharomyces cerevisiae 855705 NP_014373.1 CDS SSN8 NC_001146.6 584321 585292 R Ssn8p; Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance complement(584321..585292) Saccharomyces cerevisiae 855706 NP_878158.1 CDS YNL024C-A NC_001146.6 586603 586821 R Ynl024c-ap; Putative protein of unknown function; YNL024C-A is an essential gene complement(586603..586821) Saccharomyces cerevisiae 1466516 NP_014374.1 CDS YNL024C NC_001146.6 587108 587848 R Ynl024cp; Putative protein of unknown function with seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL024C is not an essential gene complement(587108..587848) Saccharomyces cerevisiae 855707 NP_014375.1 CDS FAP1 NC_001146.6 588264 591161 R Protein that binds to Fpr1p (FKBP12), conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; has similarity to putative transcription factors, including D. melanogaster shuttle craft and human NFX1; Fap1p complement(588264..591161) Saccharomyces cerevisiae 855708 NP_014376.1 CDS YNL022C NC_001146.6 591428 592900 R Ynl022cp; Putative protein of unknown function with seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to a single spot in the nucleus; YNL022C is not an essential gene complement(591428..592900) Saccharomyces cerevisiae 855709 NP_014377.1 CDS HDA1 NC_001146.6 593228 595348 D Putative catalytic subunit of a class II histone deacetylase complex that also contains Hda2p and Hda3p; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; Hda1p 593228..595348 Saccharomyces cerevisiae 855710 NP_014378.1 CDS ARK1 NC_001146.6 595624 597540 R Serine/threonine protein kinase involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; Ark1p complement(595624..597540) Saccharomyces cerevisiae 855711 NP_014379.1 CDS YNL019C NC_001146.6 598377 599231 R Ynl019cp; Putative protein of unknown function complement(598377..599231) Saccharomyces cerevisiae 855712 NP_014380.1 CDS YNL018C NC_001146.6 599937 601775 R Ynl018cp; Putative protein of unknown function complement(599937..601775) Saccharomyces cerevisiae 855713 NP_014382.1 CDS PUB1 NC_001146.6 602908 604269 D Pub1p; Poly(A)+ RNA-binding protein, abundant mRNP-component protein that binds mRNA and is required for stability of a number of mRNAs; not reported to associate with polyribosomes 602908..604269 Saccharomyces cerevisiae 855716 NP_014383.1 CDS PBI2 NC_001146.6 605385 605612 D Pbi2p; Cytosolic inhibitor of vacuolar proteinase B, required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion 605385..605612 Saccharomyces cerevisiae 855717 NP_014384.1 CDS HEF3 NC_001146.6 606320 609454 D Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; Hef3p 606320..609454 Saccharomyces cerevisiae 855718 NP_014386.2 CDS SPO1 NC_001146.6 609687 611666 D Spo1p; Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation join(609687..609791,609876..611666) Saccharomyces cerevisiae 855720 NP_014387.1 CDS YNL011C NC_001146.6 611837 613171 R Ynl011cp; Putative protein of unknown function; YNL011C is not an essential gene complement(611837..613171) Saccharomyces cerevisiae 855721 NP_014388.1 CDS YNL010W NC_001146.6 613636 614361 D Ynl010wp; Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation 613636..614361 Saccharomyces cerevisiae 855722 NP_014389.1 CDS IDP3 NC_001146.6 614822 616084 D Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; Idp3p 614822..616084 Saccharomyces cerevisiae 855723 NP_014390.1 CDS ASI3 NC_001146.6 616212 618221 R Putative integral membrane E3 ubiquitin ligase; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; Asi3p complement(616212..618221) Saccharomyces cerevisiae 855724 NP_014391.1 CDS SIS1 NC_001146.6 618509 619567 R Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins; Sis1p complement(618509..619567) Saccharomyces cerevisiae 855725 NP_014392.1 CDS LST8 NC_001146.6 620069 620980 D Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat; Lst8p 620069..620980 Saccharomyces cerevisiae 855726 NP_014393.1 CDS MRP7 NC_001146.6 621315 622430 R Mrp7p; Mitochondrial ribosomal protein of the large subunit complement(621315..622430) Saccharomyces cerevisiae 855727 NP_014394.2 CDS HRB1 NC_001146.6 622917 624623 D Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p; Hrb1p join(622917..622946,623289..624623) Saccharomyces cerevisiae 855728 NP_014395.1 CDS PET8 NC_001146.6 624977 625831 R S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth; Pet8p complement(624977..625831) Saccharomyces cerevisiae 855729 NP_014396.1 CDS RLP7 NC_001146.6 626176 627144 R Rlp7p; Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs complement(626176..627144) Saccharomyces cerevisiae 855730 NP_014397.1 CDS DOM34 NC_001146.6 627458 628618 D Endoribonuclease; functions in no-go mRNA decay, protein translation to promote G1 progression and differentiation, required for meiotic cell division; similar to the eukaryotic Pelota; Dom34p 627458..628618 Saccharomyces cerevisiae 855731 NP_014398.1 CDS CIT1 NC_001146.6 629624 631063 R Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; Cit1p complement(629624..631063) Saccharomyces cerevisiae 855732 NP_014399.1 CDS ATO2 NC_001146.6 633010 633858 R Putative transmembrane protein involved in export of ammonia, a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Ady2p and Y. lipolytica Gpr1p; Ato2p complement(633010..633858) Saccharomyces cerevisiae 855736 NP_014400.1 CDS RPC34 NC_001146.6 634346 635299 R Rpc34p; RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex complement(634346..635299) Saccharomyces cerevisiae 855737 NP_014401.1 CDS YNR004W NC_001146.6 635945 636385 D Ynr004wp; Putative protein of unknown function; haploid disruptant exhibits slow growth rate on glucose-minimal medium at 15 C 635945..636385 Saccharomyces cerevisiae 855738 NP_014403.1 CDS VPS27 NC_001146.6 636988 638856 D Vps27p; Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) 636988..638856 Saccharomyces cerevisiae 855739 NP_014404.1 CDS ATG3 NC_001146.6 639184 640116 R E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; Atg3p complement(639184..640116) Saccharomyces cerevisiae 855741 NP_014405.1 CDS LRO1 NC_001146.6 640398 642383 D Lro1p; Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase 640398..642383 Saccharomyces cerevisiae 855742 NP_014406.1 CDS NRM1 NC_001146.6 642692 643441 D Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase; Nrm1p 642692..643441 Saccharomyces cerevisiae 855743 NP_014407.1 CDS CSE2 NC_001146.6 643746 644195 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Med9/10 module; required for regulation of RNA polymerase II activity; Cse2p 643746..644195 Saccharomyces cerevisiae 855744 NP_014408.1 CDS PRP2 NC_001146.6 644322 646952 R RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; Prp2p complement(644322..646952) Saccharomyces cerevisiae 855745 NP_014409.1 CDS URK1 NC_001146.6 647434 648939 D Urk1p; Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP 647434..648939 Saccharomyces cerevisiae 855746 NP_014410.1 CDS PHO91 NC_001146.6 649030 651714 R Pho91p; Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth complement(649030..651714) Saccharomyces cerevisiae 855747 NP_014411.1 CDS YNR014W NC_001146.6 652467 653105 D Ynr014wp; Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible 652467..653105 Saccharomyces cerevisiae 855748 NP_014412.1 CDS SMM1 NC_001146.6 653389 654543 D Smm1p; Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs 653389..654543 Saccharomyces cerevisiae 855749 NP_014413.1 CDS ACC1 NC_001146.6 654675 661376 R Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Acc1p complement(654675..661376) Saccharomyces cerevisiae 855750 NP_014414.1 CDS TIM23 NC_001146.6 662915 663583 D Tim23p; Essential protein of the mitochondrial inner membrane, component of the mitochondrial import system 662915..663583 Saccharomyces cerevisiae 855751 NP_014415.1 CDS AIM38 NC_001146.6 664272 664946 D Putative protein of unknown function; non-tagged protein is detected in purified mitochondria; null mutant displays decreased frequency of mitochondrial genome loss (petite formation) and severe growth defect in minimal glycerol media; Aim38p 664272..664946 Saccharomyces cerevisiae 855752 NP_014416.1 CDS ARE2 NC_001146.6 665341 667269 D Are2p; Acyl-CoA:sterol acyltransferase, isozyme of Are1p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen 665341..667269 Saccharomyces cerevisiae 855753 NP_014417.1 CDS ATP23 NC_001146.6 667412 668224 R Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; Atp23p complement(667412..668224) Saccharomyces cerevisiae 855754 NP_014418.1 CDS YNR021W NC_001146.6 668379 669593 D Ynr021wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene 668379..669593 Saccharomyces cerevisiae 855755 NP_014419.1 CDS MRPL50 NC_001146.6 669776 670195 R Mrpl50p; Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation complement(669776..670195) Saccharomyces cerevisiae 855756 NP_014420.1 CDS SNF12 NC_001146.6 670420 672120 D Snf12p; 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive 670420..672120 Saccharomyces cerevisiae 855757 NP_014421.1 CDS YNR024W NC_001146.6 672411 672971 D Ynr024wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus 672411..672971 Saccharomyces cerevisiae 855758 NP_014423.1 CDS SEC12 NC_001146.6 673276 674691 R Sec12p; Guanine nucleotide exchange factor (GEF); glycosylated integral membrane protein of the endoplasmic reticulum, important for the initiation of transport vesicle budding from the endoplasmic reticulum through activation of the GTPase Sar1p complement(673276..674691) Saccharomyces cerevisiae 855760 NP_014424.1 CDS BUD17 NC_001146.6 674925 675878 D Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; Bud17p 674925..675878 Saccharomyces cerevisiae 855761 NP_014425.1 CDS CPR8 NC_001146.6 676179 677105 D Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway; Cpr8p 676179..677105 Saccharomyces cerevisiae 855762 NP_014426.1 CDS YNR029C NC_001146.6 677201 678490 R Ynr029cp; Putative protein of unknown function, deletion confers reduced fitness in saline complement(677201..678490) Saccharomyces cerevisiae 855763 NP_014427.1 CDS ALG12 NC_001146.6 678801 680456 D Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation; Alg12p 678801..680456 Saccharomyces cerevisiae 855764 NP_014428.1 CDS SSK2 NC_001146.6 680696 685435 R Ssk2p; MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress complement(680696..685435) Saccharomyces cerevisiae 855765 NP_014429.1 CDS PPG1 NC_001146.6 686012 687118 D Putative serine/threonine protein phosphatase, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases; Ppg1p 686012..687118 Saccharomyces cerevisiae 855766 NP_014430.1 CDS HUB1 NC_001146.6 687245 687466 R Ubiquitin-like protein modifier, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear; Hub1p complement(687245..687466) Saccharomyces cerevisiae 855767 NP_014431.1 CDS ABZ1 NC_001146.6 687637 690000 D Para-aminobenzoate (PABA) synthase, has similarity to Escherichia coli PABA synthase components PabA and PabB; Abz1p 687637..690000 Saccharomyces cerevisiae 855768 NP_014432.1 CDS SOL1 NC_001146.6 690323 691288 D Sol1p; Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol2p, Sol3p, and Sol4p 690323..691288 Saccharomyces cerevisiae 855769 NP_061494.1 CDS YNR034W-A NC_001146.6 692563 692859 D Ynr034w-ap; Putative protein of unknown function; expression is regulated by Msn2p/Msn4p 692563..692859 Saccharomyces cerevisiae 855770 NP_014433.1 CDS ARC35 NC_001146.6 693021 694049 R Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin; Arc35p complement(693021..694049) Saccharomyces cerevisiae 855771 NP_014434.1 CDS YNR036C NC_001146.6 694363 694824 R Ynr036cp; Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins complement(694363..694824) Saccharomyces cerevisiae 855772 NP_014435.1 CDS RSM19 NC_001146.6 695054 695329 R Rsm19p; Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein complement(695054..695329) Saccharomyces cerevisiae 855773 NP_014436.1 CDS DBP6 NC_001146.6 695597 697486 D Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; Dbp6p 695597..697486 Saccharomyces cerevisiae 855774 NP_014437.1 CDS ZRG17 NC_001146.6 697616 699433 R Zrg17p; Endoplasmic reticulum protein of unknown function, transcription is induced under conditions of zinc deficiency; mutant phenotype suggests a role in uptake of zinc complement(697616..699433) Saccharomyces cerevisiae 855775 NP_014438.1 CDS YNR040W NC_001146.6 699692 700462 D Ynr040wp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 699692..700462 Saccharomyces cerevisiae 855776 NP_014439.1 CDS COQ2 NC_001146.6 700543 701661 R Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; Coq2p complement(700543..701661) Saccharomyces cerevisiae 855778 NP_014441.1 CDS MVD1 NC_001146.6 701897 703087 D Mvd1p; Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer 701897..703087 Saccharomyces cerevisiae 855779 NP_014442.1 CDS AGA1 NC_001146.6 703701 705878 D Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; Aga1p 703701..705878 Saccharomyces cerevisiae 855780 NP_014443.1 CDS PET494 NC_001146.6 706141 707610 D Pet494p; Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane 706141..707610 Saccharomyces cerevisiae 855781 NP_014444.1 CDS TRM112 NC_001146.6 707790 708197 D Trm112p; Subunit of an adoMet-dependent tRNA methyltransferase (MTase) complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; putative zinc binding subunit of other MTase-related proteins 707790..708197 Saccharomyces cerevisiae 855782 NP_014445.1 CDS YNR047W NC_001146.6 708525 711206 D Ynr047wp; Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate 708525..711206 Saccharomyces cerevisiae 855783 NP_014446.1 CDS YNR048W NC_001146.6 711632 712813 D Ynr048wp; Protein proposed to interact with phospholipid translocases, shares similarity to Cdc50p 711632..712813 Saccharomyces cerevisiae 855784 NP_014447.1 CDS MSO1 NC_001146.6 713025 713657 R Probable component of the secretory vesicle docking complex, acts at a late step in secretion; shows genetic and physical interactions with Sec1p and is enriched in microsomal membrane fractions; required for sporulation; Mso1p complement(713025..713657) Saccharomyces cerevisiae 855785 NP_014448.1 CDS LYS9 NC_001146.6 714050 715390 R Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, which is the seventh step in lysine biosynthesis pathway; Lys9p complement(714050..715390) Saccharomyces cerevisiae 855786 NP_014449.1 CDS BRE5 NC_001146.6 716782 718329 R Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A; Bre5p complement(716782..718329) Saccharomyces cerevisiae 855787 NP_014450.1 CDS POP2 NC_001146.6 719348 720649 R RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation; Pop2p complement(719348..720649) Saccharomyces cerevisiae 855788 NP_014451.1 CDS NOG2 NC_001146.6 721122 723113 R Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; Nog2p complement(join(721122..721772,722304..723113)) Saccharomyces cerevisiae 855789 NP_014452.1 CDS ESF2 NC_001146.6 723357 724307 R Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator; Esf2p complement(723357..724307) Saccharomyces cerevisiae 855790 NP_014453.1 CDS HOL1 NC_001146.6 728427 730187 R Putative transporter in the major facilitator superfamily (DHA1 family) of multidrug resistance transporters; mutations in membrane-spanning domains permit cation and histidinol uptake; Hol1p complement(728427..730187) Saccharomyces cerevisiae 855792 NP_014454.1 CDS BIO5 NC_001146.6 731618 733303 R Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; Bio5p complement(731618..733303) Saccharomyces cerevisiae 855793 NP_014455.1 CDS BIO4 NC_001146.6 733356 734069 R Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels; Bio4p complement(733356..734069) Saccharomyces cerevisiae 855794 NP_014456.1 CDS BIO3 NC_001146.6 734291 735733 D Bio3p; 7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis 734291..735733 Saccharomyces cerevisiae 855795 NP_014457.1 CDS MNT4 NC_001146.6 736803 738545 D Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation; Mnt4p 736803..738545 Saccharomyces cerevisiae 855796 NP_014458.1 CDS FRE4 NC_001146.6 739951 742110 D Fre4p; Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels 739951..742110 Saccharomyces cerevisiae 855797 NP_014459.1 CDS YNR061C NC_001146.6 742881 743540 R Ynr061cp; Putative protein of unknown function complement(742881..743540) Saccharomyces cerevisiae 855798 NP_014460.1 CDS YNR062C NC_001146.6 744360 745343 R Ynr062cp; Putative membrane protein of unknown function complement(744360..745343) Saccharomyces cerevisiae 855799 NP_014461.1 CDS YNR063W NC_001146.6 746943 748766 D Ynr063wp; Putative zinc-cluster protein of unknown function 746943..748766 Saccharomyces cerevisiae 855800 NP_014462.1 CDS YNR064C NC_001146.6 749136 750008 R Ynr064cp; Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides complement(749136..750008) Saccharomyces cerevisiae 855801 NP_014463.1 CDS YNR065C NC_001146.6 750350 753700 R Ynr065cp; Protein of unknown function; YNR065C is not an essential gene complement(750350..753700) Saccharomyces cerevisiae 855802 NP_014464.1 CDS YNR066C NC_001146.6 753725 755035 R Ynr066cp; Putative membrane-localized protein of unknown function complement(753725..755035) Saccharomyces cerevisiae 855803 NP_014465.1 CDS DSE4 NC_001146.6 755746 759099 R Dse4p; Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother complement(755746..759099) Saccharomyces cerevisiae 855804 NP_014466.1 CDS YNR068C NC_001146.6 760067 760885 R Ynr068cp; Putative protein of unknown function complement(760067..760885) Saccharomyces cerevisiae 855805 NP_014467.1 CDS BSC5 NC_001146.6 761123 762592 R Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; Bsc5p complement(761123..762592) Saccharomyces cerevisiae 855806 NP_014468.1 CDS YNR070W NC_001146.6 765375 769376 D Ynr070wp; Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 765375..769376 Saccharomyces cerevisiae 855807 NP_014469.1 CDS YNR071C NC_001146.6 770440 771468 R Ynr071cp; Putative protein of unknown function complement(770440..771468) Saccharomyces cerevisiae 855808 NP_014470.1 CDS HXT17 NC_001146.6 772657 774351 D Hxt17p; Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose 772657..774351 Saccharomyces cerevisiae 855809 NP_014471.1 CDS YNR073C NC_001146.6 774792 776300 R Ynr073cp; Putative mannitol dehydrogenase complement(774792..776300) Saccharomyces cerevisiae 855810 NP_014472.1 CDS AIF1 NC_001146.6 777602 778738 R Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase; Aif1p complement(777602..778738) Saccharomyces cerevisiae 855811 NP_014473.1 CDS COS10 NC_001146.6 779916 781040 D Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; Cos10p 779916..781040 Saccharomyces cerevisiae 855812 NP_878159.1 CDS YNR075C-A NC_001146.6 781511 781603 R Ynr075c-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(781511..781603) Saccharomyces cerevisiae 1466517 NP_014474.1 CDS PAU6 NC_001146.6 781918 782280 D Pau6p; Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme 781918..782280 Saccharomyces cerevisiae 855813 NP_878160.1 CDS YOL166W-A NC_001147.5 585 740 D Yol166w-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 585..740 Saccharomyces cerevisiae 1466472 NP_014477.1 CDS AAD15 NC_001147.5 1647 2078 R Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; Aad15p complement(1647..2078) Saccharomyces cerevisiae 853999 NP_878161.1 CDS YOL164W-A NC_001147.5 4130 4312 D Yol164w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 4130..4312 Saccharomyces cerevisiae 1466473 NP_014478.1 CDS BDS1 NC_001147.5 6175 8115 D Bds1p; Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources 6175..8115 Saccharomyces cerevisiae 854000 NP_014479.1 CDS YOL163W NC_001147.5 9596 10105 D Yol163wp; Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family 9596..10105 Saccharomyces cerevisiae 854001 NP_014480.1 CDS YOL162W NC_001147.5 10118 10765 D Yol162wp; Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family 10118..10765 Saccharomyces cerevisiae 854002 NP_014481.1 CDS PAU20 NC_001147.5 11548 11910 R Pau20p complement(11548..11910) Saccharomyces cerevisiae 854003 NP_076909.1 CDS YOL159C-A NC_001147.5 15232 15504 R Yol159c-ap; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species complement(15232..15504) Saccharomyces cerevisiae 854005 NP_014483.1 CDS YOL159C NC_001147.5 17280 17795 R Yol159cp; Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA complement(17280..17795) Saccharomyces cerevisiae 854006 NP_014484.1 CDS ENB1 NC_001147.5 19490 21310 R Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment; Enb1p complement(19490..21310) Saccharomyces cerevisiae 854007 NP_014485.1 CDS YOL157C NC_001147.5 22524 24293 R Yol157cp; Putative protein of unknown function complement(22524..24293) Saccharomyces cerevisiae 854008 NP_014486.1 CDS HXT11 NC_001147.5 25272 26975 D Putative hexose transporter that is nearly identical to Hxt9p, has similarity to major facilitator superfamily (MFS) transporters and is involved in pleiotropic drug resistance; Hxt11p 25272..26975 Saccharomyces cerevisiae 854009 NP_878162.1 CDS YOL155W-A NC_001147.5 27083 27217 D Yol155w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 27083..27217 Saccharomyces cerevisiae 1466474 NP_014487.1 CDS HPF1 NC_001147.5 28702 31605 R Hpf1p; Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines complement(28702..31605) Saccharomyces cerevisiae 854010 NP_014488.1 CDS ZPS1 NC_001147.5 34657 35406 D Zps1p; Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH 34657..35406 Saccharomyces cerevisiae 854011 NP_014489.2 CDS FRE7 NC_001147.5 40747 42609 D Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels; Fre7p 40747..42609 Saccharomyces cerevisiae 854013 NP_014490.1 CDS GRE2 NC_001147.5 43693 44721 D NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; Gre2p 43693..44721 Saccharomyces cerevisiae 854014 NP_014492.1 CDS DCP1 NC_001147.5 44937 45632 D Subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; Dcp1p 44937..45632 Saccharomyces cerevisiae 854016 NP_014493.1 CDS SPT20 NC_001147.5 45759 47573 R Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex; Spt20p complement(45759..47573) Saccharomyces cerevisiae 854017 NP_014494.1 CDS PEX11 NC_001147.5 47932 48642 R Pex11p; Peroxisomal membrane protein required for peroxisome proliferation and medium-chain fatty acid oxidation, most abundant protein in the peroxisomal membrane, regulated by Adr1p and Pip2p-Oaf1p, promoter contains ORE and UAS1-like elements complement(47932..48642) Saccharomyces cerevisiae 854018 NP_014495.2 CDS PSF3 NC_001147.5 48863 49447 D Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery; Psf3p 48863..49447 Saccharomyces cerevisiae 854019 NP_014496.1 CDS CTR9 NC_001147.5 49554 52787 R Ctr9p; Component of the Paf1p complex, which is a large complex that binds to and modulates the activity of RNA polymerase II and is required for expression of a subset of genes, including cyclin genes; contains TPR repeats complement(49554..52787) Saccharomyces cerevisiae 854020 NP_014497.1 CDS NOP8 NC_001147.5 53097 54551 D Nucleolar protein required for 60S ribosomal subunit biogenesis; Nop8p 53097..54551 Saccharomyces cerevisiae 854021 NP_014498.1 CDS RIB4 NC_001147.5 54594 55103 R Rib4p; Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin complement(54594..55103) Saccharomyces cerevisiae 854022 NP_014499.1 CDS RRP40 NC_001147.5 55557 56279 D Rrp40p; Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex 55557..56279 Saccharomyces cerevisiae 854023 NP_014500.1 CDS PPM2 NC_001147.5 56451 58538 D Putative carboxymethyltransferase, similar to Ppm1p but biochemical activity not yet demonstrated; required for the synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; Ppm2p 56451..58538 Saccharomyces cerevisiae 854024 NP_014501.1 CDS ARG8 NC_001147.5 58759 60030 D Arg8p; Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine 58759..60030 Saccharomyces cerevisiae 854025 NP_014502.1 CDS CDC33 NC_001147.5 60383 61024 R Cytoplasmic mRNA cap binding protein; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with the translation initiation factor eIF4G (Tif4631p or Tif4632p); Cdc33p complement(60383..61024) Saccharomyces cerevisiae 854026 NP_014503.1 CDS YOL138C NC_001147.5 61325 65350 R Yol138cp; Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the vacuole complement(61325..65350) Saccharomyces cerevisiae 853982 NP_014504.1 CDS BSC6 NC_001147.5 65621 67114 D Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; Bsc6p 65621..67114 Saccharomyces cerevisiae 853983 NP_014505.1 CDS PFK27 NC_001147.5 67561 68754 R Pfk27p; 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A complement(67561..68754) Saccharomyces cerevisiae 853984 NP_014506.1 CDS MED7 NC_001147.5 69376 70044 R Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med7p complement(69376..70044) Saccharomyces cerevisiae 853985 NP_014508.1 CDS HRT1 NC_001147.5 70325 70690 D RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF; Hrt1p 70325..70690 Saccharomyces cerevisiae 853986 NP_014509.1 CDS GAS4 NC_001147.5 71300 72715 D Gas4p; 1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall 71300..72715 Saccharomyces cerevisiae 853988 NP_014510.1 CDS YOL131W NC_001147.5 73031 73357 D Yol131wp; Putative protein of unknown function 73031..73357 Saccharomyces cerevisiae 853989 NP_014511.1 CDS ALR1 NC_001147.5 74400 76979 D Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; Alr1p 74400..76979 Saccharomyces cerevisiae 853990 NP_014512.1 CDS VPS68 NC_001147.5 77560 78114 D Vps68p; Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria 77560..78114 Saccharomyces cerevisiae 853991 NP_014513.1 CDS YGK3 NC_001147.5 78352 79479 R Ygk3p; Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation complement(78352..79479) Saccharomyces cerevisiae 853992 NP_014514.1 CDS RPL25 NC_001147.5 80348 81190 D Rpl25p; Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarity to E. coli L23 and rat L23a ribosomal proteins; binds to 26S rRNA via a conserved C-terminal motif join(80348..80360,80775..81190) Saccharomyces cerevisiae 853993 NP_014515.2 CDS MDH2 NC_001147.5 81787 82920 R Mdh2p; Cytoplasmic malate dehydrogenase, one of the three isozymes that catalyze interconversion of malate and oxaloacetate; involved in gluconeogenesis during growth on ethanol or acetate as carbon source; interacts with Pck1p and Fbp1p complement(81787..82920) Saccharomyces cerevisiae 853994 NP_014516.1 CDS TRM13 NC_001147.5 83834 85264 D Trm13p; 2'-O-methyltransferase responsible for modification of tRNA at position 4; exhibits no obvious similarity to other known methyltransferases 83834..85264 Saccharomyces cerevisiae 853995 NP_014517.1 CDS TRM11 NC_001147.5 85456 86757 R Trm11p; Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain complement(85456..86757) Saccharomyces cerevisiae 853996 NP_014518.1 CDS HRP1 NC_001147.5 87844 89448 D Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; Hrp1p 87844..89448 Saccharomyces cerevisiae 853997 NP_014519.1 CDS SMF1 NC_001147.5 89692 91419 R Smf1p; Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins complement(89692..91419) Saccharomyces cerevisiae 854027 NP_014520.1 CDS RPS19A NC_001147.5 92026 92850 R Rps19ap; Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Bp complement(join(92026..92440,92831..92850)) Saccharomyces cerevisiae 854028 NP_014521.1 CDS RPL18A NC_001147.5 93395 94402 R Rpl18ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl18Bp and has similarity to rat L18 ribosomal protein; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation complement(join(93395..93843,94291..94402)) Saccharomyces cerevisiae 854029 NP_014522.1 CDS MCH4 NC_001147.5 94856 96361 R Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport; Mch4p complement(94856..96361) Saccharomyces cerevisiae 854030 NP_014524.1 CDS RRI2 NC_001147.5 97551 99488 D Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response; Rri2p 97551..99488 Saccharomyces cerevisiae 854032 NP_014525.1 CDS MSN1 NC_001147.5 99809 100957 D Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; Msn1p 99809..100957 Saccharomyces cerevisiae 854033 NP_014526.1 CDS PAP2 NC_001147.5 101475 103229 D Pap2p; Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase 101475..103229 Saccharomyces cerevisiae 854034 NP_014527.1 CDS YOL114C NC_001147.5 103317 103925 R Yol114cp; Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene complement(103317..103925) Saccharomyces cerevisiae 854035 NP_014528.1 CDS SKM1 NC_001147.5 104326 106293 D Skm1p; Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; proposed to be a downstream effector of Cdc42p during polarized growth 104326..106293 Saccharomyces cerevisiae 854036 NP_014529.1 CDS MSB4 NC_001147.5 106710 108188 D Msb4p; GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization 106710..108188 Saccharomyces cerevisiae 854037 NP_014530.1 CDS MDY2 NC_001147.5 108258 108896 R Protein required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes and interacts with YOR164C; contains a ubiquitin-like (UBL) domain; Mdy2p complement(108258..108896) Saccharomyces cerevisiae 854038 NP_014531.1 CDS SHR5 NC_001147.5 109176 109889 D Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Shr5p 109176..109889 Saccharomyces cerevisiae 854039 NP_014532.1 CDS ZEO1 NC_001147.5 110297 110638 D Zeo1p; Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria 110297..110638 Saccharomyces cerevisiae 854040 NP_014533.1 CDS INO4 NC_001147.5 111431 111886 R Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain; Ino4p complement(111431..111886) Saccharomyces cerevisiae 854042 NP_014534.1 CDS YOL107W NC_001147.5 112102 113130 D Yol107wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein 112102..113130 Saccharomyces cerevisiae 854043 NP_014536.1 CDS WSC3 NC_001147.5 114138 115808 R Wsc3p; Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis complement(114138..115808) Saccharomyces cerevisiae 854046 NP_014537.1 CDS NDJ1 NC_001147.5 116396 117454 R Ndj1p; Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution (interference), sister chromatid cohesion at meiotic telomeres, and segregation of small chromosomes complement(116396..117454) Saccharomyces cerevisiae 854047 NP_058181.1 CDS YOL103W-B NC_001147.5 118000 123268 D Yol103w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(118000..119304,119306..123268) Saccharomyces cerevisiae 854049 NP_058182.1 CDS YOL103W-A NC_001147.5 118000 119322 D Yol103w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 118000..119322 Saccharomyces cerevisiae 854048 NP_014538.2 CDS ITR2 NC_001147.5 124001 125830 D Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively; Itr2p 124001..125830 Saccharomyces cerevisiae 854050 NP_014539.1 CDS TPT1 NC_001147.5 125997 126689 R tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide; Tpt1p complement(125997..126689) Saccharomyces cerevisiae 854051 NP_014540.1 CDS IZH4 NC_001147.5 126982 127920 R Membrane protein involved in zinc metabolism, member of the four-protein IZH family, expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; Izh4p complement(126982..127920) Saccharomyces cerevisiae 854052 NP_014541.1 CDS PKH2 NC_001147.5 129237 132482 D Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; Pkh2p 129237..132482 Saccharomyces cerevisiae 854053 NP_014543.1 CDS YOL098C NC_001147.5 132725 135838 R Yol098cp; Putative metalloprotease complement(132725..135838) Saccharomyces cerevisiae 854055 NP_878163.1 CDS YOL097W-A NC_001147.5 136220 136405 D Yol097w-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry 136220..136405 Saccharomyces cerevisiae 1466475 NP_014544.1 CDS WRS1 NC_001147.5 136527 137825 R Wrs1p; Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA complement(136527..137825) Saccharomyces cerevisiae 854056 NP_014545.2 CDS COQ3 NC_001147.5 138107 139045 R O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; Coq3p complement(138107..139045) Saccharomyces cerevisiae 854057 NP_014546.1 CDS HMI1 NC_001147.5 139227 141347 R Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD; Hmi1p complement(139227..141347) Saccharomyces cerevisiae 854058 NP_014547.1 CDS RFC4 NC_001147.5 141584 142555 R Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc4p complement(141584..142555) Saccharomyces cerevisiae 854059 NP_014548.1 CDS TRM10 NC_001147.5 142815 143696 D tRNA methyltransferase, methylates the N-1 position of guanosine in tRNAs; Trm10p 142815..143696 Saccharomyces cerevisiae 854060 NP_014549.1 CDS YOL092W NC_001147.5 144204 145130 D Yol092wp; Putative protein of unknown function; predicted to contain six transmembrane domains and is 58% similar to the uncharacterized ORF YBR147W; deletion mutant has no detectable phenotype 144204..145130 Saccharomyces cerevisiae 854061 NP_014550.1 CDS SPO21 NC_001147.5 145334 147163 D Spo21p; Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation 145334..147163 Saccharomyces cerevisiae 854062 NP_014551.1 CDS MSH2 NC_001147.5 147382 150276 D Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity; Msh2p-Msh6p binds to and hydrolyzes ATP; Msh2p 147382..150276 Saccharomyces cerevisiae 854063 NP_014552.1 CDS HAL9 NC_001147.5 150398 153490 R Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; Hal9p complement(150398..153490) Saccharomyces cerevisiae 854064 NP_014553.1 CDS MPD2 NC_001147.5 153912 154745 R Mpd2p; Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p complement(153912..154745) Saccharomyces cerevisiae 854065 NP_014554.1 CDS YOL087C NC_001147.5 155287 158637 R Yol087cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid complement(155287..158637) Saccharomyces cerevisiae 854066 NP_076910.1 CDS YOL086W-A NC_001147.5 159173 159445 D Yol086w-ap; Putative protein of unknown function; identified by homology 159173..159445 Saccharomyces cerevisiae 854067 NP_014555.1 CDS ADH1 NC_001147.5 159548 160594 R Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; Adh1p complement(159548..160594) Saccharomyces cerevisiae 854068 NP_014557.1 CDS PHM7 NC_001147.5 162356 165331 D Protein of unknown function, expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; Phm7p 162356..165331 Saccharomyces cerevisiae 854070 NP_014558.1 CDS YOL083W NC_001147.5 165714 166952 D Yol083wp 165714..166952 Saccharomyces cerevisiae 854071 NP_014559.1 CDS ATG19 NC_001147.5 168727 169974 D Protein involved in the cytoplasm-to-vacuole targeting pathway and in autophagy, recognizes cargo proteins and delivers them to the preautophagosomal structure for eventual engulfment by the autophagosome and degradation; Atg19p 168727..169974 Saccharomyces cerevisiae 854072 NP_014560.1 CDS IRA2 NC_001147.5 171070 180309 D Ira2p; GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin 171070..180309 Saccharomyces cerevisiae 854073 NP_014561.1 CDS REX4 NC_001147.5 180558 181427 R Rex4p; Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly complement(180558..181427) Saccharomyces cerevisiae 854075 NP_014563.1 CDS AVO1 NC_001147.5 181682 185212 D Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth; Avo1p 181682..185212 Saccharomyces cerevisiae 854076 NP_014564.1 CDS ATP19 NC_001147.5 185438 185644 D Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase; Atp19p 185438..185644 Saccharomyces cerevisiae 854077 NP_014565.1 CDS BRX1 NC_001147.5 185848 186723 R Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif; Brx1p complement(185848..186723) Saccharomyces cerevisiae 854078 NP_014566.1 CDS MDM20 NC_001147.5 187024 189414 D Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly; Mdm20p 187024..189414 Saccharomyces cerevisiae 854079 NP_014567.1 CDS YOL075C NC_001147.5 189658 193542 R Yol075cp; Putative ABC transporter complement(189658..193542) Saccharomyces cerevisiae 854080 NP_014568.1 CDS YOL073C NC_001147.5 193832 194800 R Yol073cp; Putative protein of unknown function complement(193832..194800) Saccharomyces cerevisiae 854081 NP_014569.1 CDS THP1 NC_001147.5 194970 196337 D Thp1p; Nuclear pore-associated protein, forms a complex with Sac3p that is involved in transcription and in mRNA export from the nucleus; contains a PAM domain implicated in protein-protein binding 194970..196337 Saccharomyces cerevisiae 854082 NP_014570.1 CDS EMI5 NC_001147.5 196507 196995 D Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; Emi5p 196507..196995 Saccharomyces cerevisiae 854083 NP_014571.1 CDS NBA1 NC_001147.5 197220 198725 R Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, and bud neck; potential Cdc28p substrate; Nba1p complement(197220..198725) Saccharomyces cerevisiae 854084 NP_014572.1 CDS NUF2 NC_001147.5 198942 200297 D Nuf2p; Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering 198942..200297 Saccharomyces cerevisiae 854085 NP_014573.1 CDS HST1 NC_001147.5 200368 201879 R NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; Hst1p complement(200368..201879) Saccharomyces cerevisiae 854086 NP_014574.1 CDS RTG1 NC_001147.5 201985 202518 R Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus; Rtg1p complement(201985..202518) Saccharomyces cerevisiae 854087 NP_014575.1 CDS RIB2 NC_001147.5 202696 204471 R Rib2p; DRAP deaminase, catalyzes the third step of the riboflavin biosynthesis pathway; cytoplasmic tRNA pseudouridine synthase involved in pseudouridylation of cytoplasmic tRNAs at position 32 complement(202696..204471) Saccharomyces cerevisiae 854088 NP_014576.1 CDS INP54 NC_001147.5 204731 205885 R Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins; Inp54p complement(204731..205885) Saccharomyces cerevisiae 854089 NP_014577.1 CDS MET22 NC_001147.5 206103 207176 R Met22p; Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway complement(206103..207176) Saccharomyces cerevisiae 854090 NP_014578.1 CDS CRT10 NC_001147.5 207392 210265 R Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat; Crt10p complement(207392..210265) Saccharomyces cerevisiae 854091 NP_014579.1 CDS APM4 NC_001147.5 210520 211995 R Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport; Apm4p complement(210520..211995) Saccharomyces cerevisiae 854092 NP_014580.1 CDS PRS5 NC_001147.5 212244 213734 D Prs5p; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes 212244..213734 Saccharomyces cerevisiae 854093 NP_014581.1 CDS MAM3 NC_001147.5 214017 216137 R Protein required for normal mitochondrial morphology, has similarity to hemolysins; Mam3p complement(214017..216137) Saccharomyces cerevisiae 854094 NP_014582.1 CDS GPD2 NC_001147.5 217126 218448 D NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; Gpd2p 217126..218448 Saccharomyces cerevisiae 854095 NP_014583.1 CDS ARG1 NC_001147.5 219210 220472 D Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate; Arg1p 219210..220472 Saccharomyces cerevisiae 854096 NP_014584.1 CDS YOL057W NC_001147.5 220766 222901 D Yol057wp; Putative metalloprotease 220766..222901 Saccharomyces cerevisiae 854097 NP_014585.1 CDS GPM3 NC_001147.5 223267 224178 D Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event; Gpm3p 223267..224178 Saccharomyces cerevisiae 854098 NP_014586.1 CDS THI20 NC_001147.5 224419 226074 R Thi20p; Multifunctional protein with both hydroxymethylpyrimidine kinase and thiaminase activities; involved in thiamine biosynthesis and also in thiamine degradation; member of a gene family with THI21 and THI22; functionally redundant with Thi21p complement(224419..226074) Saccharomyces cerevisiae 854099 NP_014587.1 CDS PSH1 NC_001147.5 228613 229833 D Psh1p; Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding protein 228613..229833 Saccharomyces cerevisiae 854102 NP_014588.1 CDS AIM39 NC_001147.5 230084 231271 D Putative protein of unknown function; YOL053W is not an essential gene; null mutant displays decreased frequency of mitochondrial genome loss (petite formation); Aim39p 230084..231271 Saccharomyces cerevisiae 854103 NP_014589.1 CDS DDR2 NC_001147.5 231569 231754 R Multistress response protein, expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; Ddr2p complement(231569..231754) Saccharomyces cerevisiae 854104 NP_014590.1 CDS SPE2 NC_001147.5 232445 233635 R Spe2p; S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically complement(232445..233635) Saccharomyces cerevisiae 854105 NP_014591.1 CDS GAL11 NC_001147.5 234939 238184 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; Gal11p 234939..238184 Saccharomyces cerevisiae 854106 NP_014593.1 CDS GSH2 NC_001147.5 238618 240093 D Gsh2p; Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock 238618..240093 Saccharomyces cerevisiae 854108 NP_014594.2 CDS YOL048C NC_001147.5 240203 241309 R Yol048cp; Putative protein of unknown function complement(join(240203..240946,241025..241309)) Saccharomyces cerevisiae 854109 NP_014595.2 CDS YOL047C NC_001147.5 241613 242746 R Yol047cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(join(241613..242440,242504..242746)) Saccharomyces cerevisiae 854110 NP_014597.1 CDS PSK2 NC_001147.5 243496 246801 D Psk2p; One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation) 243496..246801 Saccharomyces cerevisiae 854111 NP_014598.1 CDS PEX15 NC_001147.5 247149 248300 D Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly; Pex15p 247149..248300 Saccharomyces cerevisiae 854113 NP_014599.1 CDS NTG2 NC_001147.5 248391 249533 R Ntg2p; DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus complement(248391..249533) Saccharomyces cerevisiae 854114 NP_014600.1 CDS NGL1 NC_001147.5 249824 250915 D Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Ngl1p 249824..250915 Saccharomyces cerevisiae 854115 NP_014601.1 CDS NOP12 NC_001147.5 251266 252645 R Nucleolar protein, required for pre-25S rRNA processing; contains an RNA recognition motif (RRM) and has similarity to Nop13p, Nsr1p, and putative orthologs in Drosophila and S. pombe; Nop12p complement(251266..252645) Saccharomyces cerevisiae 854116 NP_014602.1 CDS RPS15 NC_001147.5 253148 253576 R Rps15p; Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins complement(253148..253576) Saccharomyces cerevisiae 854117 NP_014603.1 CDS RPP2A NC_001147.5 254296 254616 D Rpp2ap; Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm 254296..254616 Saccharomyces cerevisiae 854118 NP_878164.1 CDS YOL038C-A NC_001147.5 254925 255020 R Yol038c-ap; Putative protein of unknown function; identified by SAGE analysis complement(254925..255020) Saccharomyces cerevisiae 1466476 NP_014604.1 CDS PRE6 NC_001147.5 255336 256100 D Pre6p; 20S proteasome alpha-type subunit; green fluorescent protein (GFP)-fusion protein relocates from the cytosol to the mitochondrial outer surface upon oxidative stress 255336..256100 Saccharomyces cerevisiae 854119 NP_014606.1 CDS YOL036W NC_001147.5 256744 259029 D Yol036wp; Protein of unknown function; potential Cdc28p substrate 256744..259029 Saccharomyces cerevisiae 854120 NP_014608.1 CDS SMC5 NC_001147.5 259924 263205 D Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair; Smc5p 259924..263205 Saccharomyces cerevisiae 854123 NP_014609.1 CDS MSE1 NC_001147.5 263476 265086 D Mse1p; Mitochondrial glutamyl-tRNA synthetase, encoded by a nuclear gene 263476..265086 Saccharomyces cerevisiae 854124 NP_014610.1 CDS OPI10 NC_001147.5 265430 266170 D Protein with a possible role in phospholipid biosynthesis, based on inositol-excreting phenotype of the null mutant and its suppression by exogenous choline; Opi10p 265430..266170 Saccharomyces cerevisiae 854125 NP_014611.1 CDS SIL1 NC_001147.5 266265 267530 R Sil1p; Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein complement(266265..267530) Saccharomyces cerevisiae 854126 NP_014612.1 CDS GAS5 NC_001147.5 268188 269642 D Gas5p; 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall 268188..269642 Saccharomyces cerevisiae 854127 NP_014613.1 CDS YOL029C NC_001147.5 269816 270421 R Yol029cp; Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens complement(269816..270421) Saccharomyces cerevisiae 854128 NP_014614.1 CDS YAP7 NC_001147.5 270634 271371 R Yap7p; Putative basic leucine zipper (bZIP) transcription factor complement(270634..271371) Saccharomyces cerevisiae 854129 NP_014615.1 CDS MDM38 NC_001147.5 272004 273725 R Mitochondrial inner membrane protein, involved in membrane integration of a subset of mitochondrial proteins; required for K+/H+ exchange; associates with mitochondrial ribosomes; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome; Mdm38p complement(272004..273725) Saccharomyces cerevisiae 854130 NP_014616.1 CDS MIM1 NC_001147.5 274013 274354 R Mitochondrial outer membrane protein, required for assembly of the translocase of the outer membrane (TOM) complex and thereby for mitochondrial protein import; N terminus is exposed to the cytosol: transmembrane segment is highly conserved; Mim1p complement(274013..274354) Saccharomyces cerevisiae 854131 NP_014617.1 CDS LAG2 NC_001147.5 274958 276940 D Protein involved in determination of longevity; LAG2 gene is preferentially expressed in young cells; overexpression extends the mean and maximum life span of cells; Lag2p 274958..276940 Saccharomyces cerevisiae 854133 NP_014618.1 CDS YOL024W NC_001147.5 277087 277605 D Yol024wp; Putative protein of unknown function, predicted to have thiol-disulfide oxidoreductase active site 277087..277605 Saccharomyces cerevisiae 854134 NP_014619.1 CDS IFM1 NC_001147.5 278057 280087 D Mitochondrial translation initiation factor 2; Ifm1p 278057..280087 Saccharomyces cerevisiae 854135 NP_014620.1 CDS TSR4 NC_001147.5 280273 281499 R Tsr4p; Cytoplasmic protein of unknown function; essential gene in S288C, and non-essential with reduced growth rate in CEN.PK2; Null mutant accumulates 20S pre-rRNA complement(280273..281499) Saccharomyces cerevisiae 854136 NP_014621.1 CDS DIS3 NC_001147.5 282422 285427 R Dis3p; Catalytic component of the exosome, involved in RNA processing and degradation; binds Gsp1p/Ran and enhances the GEF activity of Srm1p; implicated in mitotic control; homologous to the E. coli RNase R of the RNase II family complement(282422..285427) Saccharomyces cerevisiae 854138 NP_014622.1 CDS TAT2 NC_001147.5 286173 287951 D Tat2p; High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance 286173..287951 Saccharomyces cerevisiae 854139 NP_878165.1 CDS YOL019W-A NC_001147.5 288421 288573 D Yol019w-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 288421..288573 Saccharomyces cerevisiae 1466477 NP_014623.1 CDS YOL019W NC_001147.5 288899 290554 D Yol019wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole 288899..290554 Saccharomyces cerevisiae 854141 NP_014624.1 CDS TLG2 NC_001147.5 290882 292075 R Tlg2p; Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation complement(290882..292075) Saccharomyces cerevisiae 854142 NP_014625.1 CDS ESC8 NC_001147.5 292530 294674 D Protein involved in telomeric and mating-type locus silencing, interacts with Sir2p and also interacts with the Gal11p, which is a component of the RNA pol II mediator complex; Esc8p 292530..294674 Saccharomyces cerevisiae 854143 NP_014626.1 CDS CMK2 NC_001147.5 294778 296121 R Cmk2p; Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk1p and mammalian Cam Kinase II complement(294778..296121) Saccharomyces cerevisiae 854144 NP_014627.1 CDS IRC10 NC_001147.5 297079 298839 D hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci; Irc10p 297079..298839 Saccharomyces cerevisiae 854145 NP_014628.1 CDS YOL014W NC_001147.5 299694 300068 D Yol014wp; Putative protein of unknown function 299694..300068 Saccharomyces cerevisiae 854146 NP_878166.1 CDS YOL013W-A NC_001147.5 301048 301239 D Yol013w-ap; Putative protein of unknown function; identified by SAGE 301048..301239 Saccharomyces cerevisiae 1466478 NP_014630.1 CDS HRD1 NC_001147.5 301381 303036 R Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; Hrd1p complement(301381..303036) Saccharomyces cerevisiae 854149 NP_014631.1 CDS HTZ1 NC_001147.5 303580 303984 R Htz1p; Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin complement(303580..303984) Saccharomyces cerevisiae 854150 NP_014632.1 CDS PLB3 NC_001147.5 305350 307410 D Plb3p; Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro 305350..307410 Saccharomyces cerevisiae 854151 NP_014633.1 CDS RCL1 NC_001147.5 307939 309042 D Rcl1p; RNA terminal phosphate cyclase-like protein involved in rRNA processing at sites A0, A1, and A2; does not possess detectable RNA cyclase activity 307939..309042 Saccharomyces cerevisiae 854152 NP_014634.1 CDS MDM12 NC_001147.5 309325 310140 R Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the Mdm10-Mdm12-Mmm1 complex involved in import and assembly of outer membrane beta-barrel proteins; Mdm12p complement(309325..310140) Saccharomyces cerevisiae 854153 NP_014635.1 CDS COQ10 NC_001147.5 310313 310936 D Coq10p; Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes 310313..310936 Saccharomyces cerevisiae 854154 NP_014636.1 CDS CSI2 NC_001147.5 311343 312368 R Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene; Csi2p complement(311343..312368) Saccharomyces cerevisiae 854155 NP_014637.1 CDS TOP1 NC_001147.5 313079 315388 R Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; Top1p complement(313079..315388) Saccharomyces cerevisiae 854156 NP_014638.1 CDS RPB11 NC_001147.5 315814 316176 R Rpb11p; RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit complement(315814..316176) Saccharomyces cerevisiae 854157 NP_014639.1 CDS SIN3 NC_001147.5 316939 321549 D Sin3p; Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity 316939..321549 Saccharomyces cerevisiae 854158 NP_014640.1 CDS PFA4 NC_001147.5 321859 322995 R Pfa4p; Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases complement(321859..322995) Saccharomyces cerevisiae 854159 NP_014641.2 CDS IZH2 NC_001147.5 323412 324365 R Plasma membrane protein involved in zinc metabolism and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc; Izh2p complement(323412..324365) Saccharomyces cerevisiae 854160 NP_014642.1 CDS PHO80 NC_001147.5 325250 326131 D Pho80p; Cyclin, negatively regulates phosphate metabolism; Pho80p-Pho85p (cyclin-CDK complex) phosphorylates Pho4p and Swi5p; deletion of PHO80 leads to aminoglycoside supersensitivity; truncated form of PHO80 affects vacuole inheritance 325250..326131 Saccharomyces cerevisiae 854161 NP_014643.1 CDS RRP6 NC_001147.5 326833 329034 D Rrp6p; Exonuclease component of the nuclear exosome; contributes to the quality-control system that retains and degrades aberrant mRNAs in the nucleus 326833..329034 Saccharomyces cerevisiae 854162 NP_014644.1 CDS ALG6 NC_001147.5 329418 331052 D Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease; Alg6p 329418..331052 Saccharomyces cerevisiae 854163 NP_014645.1 CDS YSP3 NC_001147.5 331456 332892 D Ysp3p; Putative precursor to the subtilisin-like protease III 331456..332892 Saccharomyces cerevisiae 854164 NP_014646.1 CDS UTP23 NC_001147.5 333593 334357 D Utp23p; Essential nucleolar protein that is a component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function 333593..334357 Saccharomyces cerevisiae 854165 NP_014647.1 CDS DNL4 NC_001147.5 334510 337344 R Dnl4p; DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth complement(334510..337344) Saccharomyces cerevisiae 854166 NP_014648.1 CDS TSR3 NC_001147.5 337681 338622 R Tsr3p; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; Null mutant accumulates 20S pre-rRNA complement(337681..338622) Saccharomyces cerevisiae 854167 NP_014649.1 CDS SGT2 NC_001147.5 338939 339979 R Sgt2p; Glutamine-rich cytoplasmic protein of unknown function; contains tetratricopeptide (TPR) repeats, which often mediate protein-protein interactions; has similarity to human SGT, which is a cochaperone that negatively regulates Hsp70 complement(338939..339979) Saccharomyces cerevisiae 854168 NP_014650.1 CDS SLG1 NC_001147.5 341279 342415 R Slg1p; Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway involved in maintenance of cell wall integrity; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response complement(341279..342415) Saccharomyces cerevisiae 854170 NP_014651.1 CDS YOR008C-A NC_001147.5 342858 343082 R Yor008c-ap; Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres complement(342858..343082) Saccharomyces cerevisiae 854171 NP_014652.1 CDS TIR4 NC_001147.5 344335 345798 D Tir4p; Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock 344335..345798 Saccharomyces cerevisiae 854173 NP_014653.1 CDS TIR2 NC_001147.5 346195 346950 R Tir2p; Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis complement(346195..346950) Saccharomyces cerevisiae 854174 NP_014654.1 CDS AUS1 NC_001147.5 349679 353863 D Transporter of the ATP-binding cassette family, involved in uptake of sterols and anaerobic growth; Aus1p 349679..353863 Saccharomyces cerevisiae 854175 NP_878167.1 CDS YOR011W-A NC_001147.5 355652 355858 D Yor011w-ap; Putative protein of unknown function 355652..355858 Saccharomyces cerevisiae 1466479 NP_014655.1 CDS YOR012W NC_001147.5 356543 356956 D Yor012wp; Putative protein of unknown function 356543..356956 Saccharomyces cerevisiae 854177 NP_014657.1 CDS RTS1 NC_001147.5 357674 359947 D Rts1p; B-type regulatory subunit of protein phosphatase 2A (PP2A); homolog of the mammalian B' subunit of PP2A 357674..359947 Saccharomyces cerevisiae 854179 NP_014659.1 CDS ERP4 NC_001147.5 360461 361084 R Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; Erp4p complement(360461..361084) Saccharomyces cerevisiae 854181 NP_014660.1 CDS PET127 NC_001147.5 361412 363814 D Protein with a role in mitochondrial RNA stability and/or processing, located in the mitochondrial membrane; Pet127p 361412..363814 Saccharomyces cerevisiae 854182 NP_014661.1 CDS ROD1 NC_001147.5 364369 366882 D Rod1p; Membrane protein; overexpression confers resistance to the GST substrate o-dinitrobenzene as well as to zinc and calcium; contains 2 PY motifs, which are required for Rod1p interaction with Rsp5p, a hect-type ubiquitin ligase 364369..366882 Saccharomyces cerevisiae 854183 NP_014662.1 CDS YOR019W NC_001147.5 368127 370319 D Yor019wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments 368127..370319 Saccharomyces cerevisiae 854184 NP_014663.1 CDS HSP10 NC_001147.5 370524 370844 R Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES; Hsp10p complement(370524..370844) Saccharomyces cerevisiae 854185 NP_878168.1 CDS YOR020W-A NC_001147.5 371685 371957 D Yor020w-ap; Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 371685..371957 Saccharomyces cerevisiae 1466480 NP_014664.1 CDS YOR021C NC_001147.5 372798 373439 R Yor021cp; Putative protein of unknown function; YOR021C is not an essential gene complement(372798..373439) Saccharomyces cerevisiae 854186 NP_014665.1 CDS YOR022C NC_001147.5 373710 375857 R Yor022cp; Protein with similarity to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(373710..375857) Saccharomyces cerevisiae 854187 NP_014666.1 CDS AHC1 NC_001147.5 376012 377712 R Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex; Ahc1p complement(376012..377712) Saccharomyces cerevisiae 854188 NP_014668.1 CDS HST3 NC_001147.5 378219 379562 D Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; Hst3p 378219..379562 Saccharomyces cerevisiae 854190 NP_014669.1 CDS BUB3 NC_001147.5 379781 380806 D Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; Bub3p 379781..380806 Saccharomyces cerevisiae 854191 NP_014670.1 CDS STI1 NC_001147.5 381053 382822 D Sti1p; Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones; activates the ATPase activity of Ssa1p; homolog of mammalian Hop protein 381053..382822 Saccharomyces cerevisiae 854192 NP_014671.1 CDS CIN5 NC_001147.5 383533 384420 R Cin5p; Basic leucine zipper transcriptional factor of the yAP-1 family that mediates pleiotropic drug resistance and salt tolerance; localizes constitutively to the nucleus complement(383533..384420) Saccharomyces cerevisiae 854193 NP_014673.1 CDS DFG16 NC_001147.5 386825 388684 D Probable multiple transmembrane protein, involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; required for accumulation of processed Rim101p; Dfg16p 386825..388684 Saccharomyces cerevisiae 854195 NP_014674.1 CDS CRS5 NC_001147.5 389213 389422 D Crs5p; Copper-binding metallothionein, required for wild-type copper resistance 389213..389422 Saccharomyces cerevisiae 854196 NP_014675.1 CDS HMS1 NC_001147.5 389771 391075 R Hms1p; Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant complement(389771..391075) Saccharomyces cerevisiae 854197 NP_878169.1 CDS YOR032W-A NC_001147.5 392176 392376 D Yor032w-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 392176..392376 Saccharomyces cerevisiae 1466481 NP_014676.1 CDS EXO1 NC_001147.5 392416 394524 R Exo1p; 5'-3' exonuclease and flap-endonuclease involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains complement(392416..394524) Saccharomyces cerevisiae 854198 NP_014677.1 CDS AKR2 NC_001147.5 394837 397086 R Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; Akr2p complement(394837..397086) Saccharomyces cerevisiae 854199 NP_878170.1 CDS YOR034C-A NC_001147.5 397426 397668 R Yor034c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(397426..397668) Saccharomyces cerevisiae 1466482 NP_014678.1 CDS SHE4 NC_001147.5 397735 400104 R She4p; Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization complement(397735..400104) Saccharomyces cerevisiae 854200 NP_014679.1 CDS PEP12 NC_001147.5 400348 401214 D Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin; Pep12p 400348..401214 Saccharomyces cerevisiae 854201 NP_014680.2 CDS CYC2 NC_001147.5 401555 402655 D Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p); Cyc2p 401555..402655 Saccharomyces cerevisiae 854202 NP_014681.1 CDS HIR2 NC_001147.5 402761 405388 R Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; Hir2p complement(402761..405388) Saccharomyces cerevisiae 854203 NP_014682.1 CDS CKB2 NC_001147.5 405768 406544 D Ckb2p; Beta' regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB1, the many substrates include transcription factors and all RNA polymerases 405768..406544 Saccharomyces cerevisiae 854204 NP_014683.1 CDS GLO4 NC_001147.5 407064 407921 D Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; Glo4p 407064..407921 Saccharomyces cerevisiae 854205 NP_014685.1 CDS CUE5 NC_001147.5 408425 409660 D Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Cue5p 408425..409660 Saccharomyces cerevisiae 854206 NP_014686.1 CDS WHI2 NC_001147.5 410870 412330 D Whi2p; Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression 410870..412330 Saccharomyces cerevisiae 854208 NP_014687.1 CDS IRC23 NC_001147.5 413007 413480 D Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52 foci; Irc23p 413007..413480 Saccharomyces cerevisiae 854209 NP_014688.1 CDS TOM6 NC_001147.5 413852 414037 D Tom6p; Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex 413852..414037 Saccharomyces cerevisiae 854210 NP_014689.1 CDS DBP5 NC_001147.5 414460 415908 R Dbp5p; Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family involved in mRNA export from the nucleus; involved in translation termination complement(414460..415908) Saccharomyces cerevisiae 854211 NP_014690.1 CDS STD1 NC_001147.5 416348 417682 R Std1p; Protein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt15p; acts as a regulator of the transcription factor Rgt1p complement(416348..417682) Saccharomyces cerevisiae 854212 NP_014691.1 CDS RAT1 NC_001147.5 418631 421651 R Rat1p; Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as mRNA transcription termination complement(418631..421651) Saccharomyces cerevisiae 854213 NP_014692.1 CDS RSB1 NC_001147.5 422669 423733 R Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane; Rsb1p complement(422669..423733) Saccharomyces cerevisiae 854214 NP_014694.1 CDS YOR051C NC_001147.5 424848 426086 R Yor051cp; Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts complement(424848..426086) Saccharomyces cerevisiae 854216 NP_014695.1 CDS YOR052C NC_001147.5 426774 427226 R Yor052cp; Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid complement(426774..427226) Saccharomyces cerevisiae 854217 NP_014697.1 CDS VHS3 NC_001147.5 427835 429859 R Vhs3p; Functionally redundant (see also SIS2) inhibitory subunit of Ppz1p, a PP1c-related ser/thr protein phosphatase Z isoform; synthetically lethal with sis2; putative phosphopantothenoylcysteine decarboxylase involved in coenzyme A biosynthesis complement(427835..429859) Saccharomyces cerevisiae 854220 NP_014699.1 CDS NOB1 NC_001147.5 430249 431628 R Nob1p; Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA complement(430249..431628) Saccharomyces cerevisiae 854221 NP_014700.1 CDS SGT1 NC_001147.5 432188 433375 D Sgt1p; Probable cochaperone, regulates activity of Cyr1p (adenylyl cyclase); involved in assembly of the kinetochore complex, associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex 432188..433375 Saccharomyces cerevisiae 854222 NP_116582.1 CDS ASE1 NC_001147.5 433690 436347 R Mitotic spindle midzone localized microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; Ase1p complement(433690..436347) Saccharomyces cerevisiae 854223 NP_014702.1 CDS YOR059C NC_001147.5 438908 440260 R Yor059cp complement(438908..440260) Saccharomyces cerevisiae 854225 NP_014703.1 CDS YOR060C NC_001147.5 440392 441165 R Yor060cp; Protein of unknown function required for cell viability; deletion mutant harbors aberrant mitochondria complement(440392..441165) Saccharomyces cerevisiae 854226 NP_014704.1 CDS CKA2 NC_001147.5 441536 442555 D Cka2p; Alpha' catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases 441536..442555 Saccharomyces cerevisiae 854227 NP_014705.1 CDS YOR062C NC_001147.5 442727 443533 R Yor062cp; Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS complement(442727..443533) Saccharomyces cerevisiae 854228 NP_014706.1 CDS RPL3 NC_001147.5 444688 445851 D Rpl3p; Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus 444688..445851 Saccharomyces cerevisiae 854229 NP_014707.1 CDS YNG1 NC_001147.5 446081 446740 R Yng1p; Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 complement(446081..446740) Saccharomyces cerevisiae 854230 NP_014708.1 CDS CYT1 NC_001147.5 447441 448370 D Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex; Cyt1p 447441..448370 Saccharomyces cerevisiae 854231 NP_014709.1 CDS MSA1 NC_001147.5 449438 451327 D Msa1p; Activator of G1-specific transcription factors, MBF and SBF, that regulates both the timing of G1-specific gene transcription, and cell cycle initiation; potential Cdc28p substrate 449438..451327 Saccharomyces cerevisiae 854232 NP_014710.1 CDS ALG8 NC_001147.5 451731 453464 R Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p; Alg8p complement(451731..453464) Saccharomyces cerevisiae 854233 NP_014712.2 CDS VPS5 NC_001147.5 453770 455797 D Vps5p; Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p 453770..455797 Saccharomyces cerevisiae 854235 NP_014711.1 CDS VAM10 NC_001147.5 453871 454215 R Vam10p; Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering complement(453871..454215) Saccharomyces cerevisiae 854234 NP_014713.1 CDS GYP1 NC_001147.5 455909 457822 R Gyp1p; Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion complement(455909..457822) Saccharomyces cerevisiae 854236 NP_014714.1 CDS THI71 NC_001147.5 459482 461278 R Transporter of thiamine or related compound; shares sequence similarity to Thi7p; proposed to be involved in 5-fluorocytosine sensitivity; Thi71p complement(459482..461278) Saccharomyces cerevisiae 854237 NP_878171.1 CDS YOR072W-B NC_001147.5 464471 464632 D Yor072w-bp; Putative protein of unknown function; identified by expression profiling and mass spectrometry 464471..464632 Saccharomyces cerevisiae 1466483 NP_014716.1 CDS SGO1 NC_001147.5 464773 466545 D Sgo1p; Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability 464773..466545 Saccharomyces cerevisiae 854240 NP_014717.2 CDS CDC21 NC_001147.5 466677 467591 R Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; Cdc21p complement(466677..467591) Saccharomyces cerevisiae 854241 NP_014718.1 CDS UFE1 NC_001147.5 468214 469254 D t-SNARE required for ER membrane fusion and vesicular traffic, integral membrane protein that constitutes with Sec20p and Use1p the trimeric acceptor for R/v-SNAREs on Golgi-derived vesicles at the ER; part of Dsl1p complex; Ufe1p 468214..469254 Saccharomyces cerevisiae 854242 NP_014719.1 CDS SKI7 NC_001147.5 469379 471622 R Ski7p; Putative GTPase that mediates interactions via its N-terminus between the exosome and Ski complex (Ski2p, Ski3p, Ski8p) which operate in the 3'-to-5' mRNA-decay pathway; cytoplasmic protein required for degrading nonstop mRNAs complement(469379..471622) Saccharomyces cerevisiae 854243 NP_014720.1 CDS RTS2 NC_001147.5 471901 472599 D Rts2p; Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication 471901..472599 Saccharomyces cerevisiae 854244 NP_014721.1 CDS BUD21 NC_001147.5 472727 473371 D Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern; Bud21p 472727..473371 Saccharomyces cerevisiae 854245 NP_014722.1 CDS ATX2 NC_001147.5 473477 474418 R Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation; Atx2p complement(473477..474418) Saccharomyces cerevisiae 854246 NP_014723.1 CDS DIA2 NC_001147.5 474554 476794 D Origin-binding F-box protein that forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication, involved in invasive and pseudohyphal growth; Dia2p 474554..476794 Saccharomyces cerevisiae 854247 NP_014724.1 CDS TGL5 NC_001147.5 476941 479190 R Triacylglycerol lipase involved in TAG mobilization; localizes to lipid particles; potential Cdc28p substrate; Tgl5p complement(476941..479190) Saccharomyces cerevisiae 854248 NP_014726.1 CDS WHI5 NC_001147.5 479535 480422 D Whi5p; Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 479535..480422 Saccharomyces cerevisiae 854249 NP_014727.1 CDS LPX1 NC_001147.5 480589 481752 D Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction; Lpx1p 480589..481752 Saccharomyces cerevisiae 854251 NP_014728.1 CDS OST3 NC_001147.5 482035 483087 D Ost3p; Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins 482035..483087 Saccharomyces cerevisiae 854252 NP_014729.1 CDS TCB1 NC_001147.5 483221 486781 R Tcb1p; Lipid-binding protein containing three calcium and lipid binding domains; non-tagged protein localizes to mitochondria and GFP-fusion protein localizes to the cell periphery; C-termini of Tcb1p, Tcb2p and Tcb3p interact complement(483221..486781) Saccharomyces cerevisiae 854253 NP_014730.2 CDS YVC1 NC_001147.5 487709 489736 D Yvc1p; Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock 487709..489736 Saccharomyces cerevisiae 854255 NP_014732.1 CDS VPS21 NC_001147.5 490198 490830 R Vps21p; GTPase required for transport during endocytosis and for correct sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog complement(490198..490830) Saccharomyces cerevisiae 854256 NP_014733.1 CDS PTC5 NC_001147.5 491125 492843 R Ptc5p; Mitochondrially localized type 2C protein phosphatase involved in regulation of pyruvate dehydrogenase activity; contains Mg2+/Mn2+-dependent casein phosphatase activity in vitro complement(491125..492843) Saccharomyces cerevisiae 854257 NP_014734.2 CDS TMA46 NC_001147.5 493434 494471 D Tma46p; Protein of unknown function that associates with ribosomes; interacts with GTPase Rbg1p 493434..494471 Saccharomyces cerevisiae 854258 NP_014735.1 CDS ECM3 NC_001147.5 495128 496969 D Non-essential protein of unknown function; Ecm3p 495128..496969 Saccharomyces cerevisiae 854259 NP_014736.1 CDS YOR093C NC_001147.5 497507 502453 R Yor093cp; Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents complement(497507..502453) Saccharomyces cerevisiae 854260 NP_014737.1 CDS ARF3 NC_001147.5 502796 503347 D Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; Arf3p 502796..503347 Saccharomyces cerevisiae 854261 NP_014738.1 CDS RKI1 NC_001147.5 503553 504329 R Rki1p; Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis complement(503553..504329) Saccharomyces cerevisiae 854262 NP_014739.1 CDS RPS7A NC_001147.5 505795 506768 D Rps7ap; Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins join(505795..505938,506340..506768) Saccharomyces cerevisiae 854263 NP_014740.1 CDS YOR097C NC_001147.5 506979 507506 R Yor097cp; Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene complement(506979..507506) Saccharomyces cerevisiae 854264 NP_014741.1 CDS NUP1 NC_001147.5 507949 511179 R Nup1p; Nuclear pore complex (NPC) subunit, involved in protein import/export and in export of RNAs, possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; potential Cdc28p substrate complement(507949..511179) Saccharomyces cerevisiae 854265 NP_014742.1 CDS KTR1 NC_001147.5 511826 513007 D Ktr1p; Alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family 511826..513007 Saccharomyces cerevisiae 854266 NP_014743.1 CDS CRC1 NC_001147.5 513296 514279 R Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation; Crc1p complement(513296..514279) Saccharomyces cerevisiae 854267 NP_014744.1 CDS RAS1 NC_001147.5 515245 516174 D Ras1p; GTPase involved in G-protein signaling in the adenylate cyclase activating pathway, plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes 515245..516174 Saccharomyces cerevisiae 854268 NP_014746.2 CDS OST2 NC_001147.5 516450 516842 R Ost2p; Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins complement(516450..516842) Saccharomyces cerevisiae 854270 NP_014747.1 CDS PIN2 NC_001147.5 517643 518491 D Protein that induces appearance of [PIN+] prion when overproduced; Pin2p 517643..518491 Saccharomyces cerevisiae 854271 NP_014749.1 CDS VAM3 NC_001147.5 519122 519973 D Vam3p; Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein trafficking; member of the syntaxin family of proteins 519122..519973 Saccharomyces cerevisiae 854273 NP_014750.1 CDS RGS2 NC_001147.5 521354 522283 D Rgs2p; Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p 521354..522283 Saccharomyces cerevisiae 854274 NP_014751.1 CDS LEU9 NC_001147.5 523028 524842 D Leu9p; Alpha-isopropylmalate synthase II (2-isopropylmalate synthase), catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant 523028..524842 Saccharomyces cerevisiae 854275 NP_014752.1 CDS INP53 NC_001147.5 525279 528602 D Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; Inp53p 525279..528602 Saccharomyces cerevisiae 854276 NP_014753.1 CDS TFC7 NC_001147.5 528942 530249 D Tfc7p; One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes 528942..530249 Saccharomyces cerevisiae 854277 NP_014754.1 CDS YOR111W NC_001147.5 530430 531128 D Yor111wp; Putative protein of unknown function 530430..531128 Saccharomyces cerevisiae 854278 NP_014755.1 CDS CEX1 NC_001147.5 531509 533794 D Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p; Cex1p 531509..533794 Saccharomyces cerevisiae 854279 NP_014756.1 CDS AZF1 NC_001147.5 534076 536820 D Zinc-finger transcription factor, involved in induction of CLN3 transcription in response to glucose; genetic and physical interactions indicate a possible role in mitochondrial transcription or genome maintenance; Azf1p 534076..536820 Saccharomyces cerevisiae 854280 NP_014757.1 CDS YOR114W NC_001147.5 537571 538455 D Yor114wp 537571..538455 Saccharomyces cerevisiae 854281 NP_014758.1 CDS TRS33 NC_001147.5 538660 539466 R Trs33p; One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic complement(538660..539466) Saccharomyces cerevisiae 854282 NP_014759.1 CDS RPO31 NC_001147.5 539764 544146 R Rpo31p; RNA polymerase III subunit C160, part of core enzyme; similar to bacterial beta-prime subunit complement(539764..544146) Saccharomyces cerevisiae 854283 NP_014760.1 CDS RPT5 NC_001147.5 545030 546334 D Rpt5p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription 545030..546334 Saccharomyces cerevisiae 854284 NP_014761.1 CDS YOR118W NC_001147.5 546858 548561 D Yor118wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR118W is not an essential gene 546858..548561 Saccharomyces cerevisiae 854285 NP_014762.1 CDS RIO1 NC_001147.5 548793 550247 R Rio1p; Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA complement(548793..550247) Saccharomyces cerevisiae 854286 NP_014763.1 CDS GCY1 NC_001147.5 551115 552053 D Putative NADP(+) coupled glycerol dehydrogenase, proposed to be involved in an alternative pathway for glycerol catabolism; member of the aldo-keto reductase (AKR) family; Gcy1p 551115..552053 Saccharomyces cerevisiae 854287 NP_014765.1 CDS PFY1 NC_001147.5 552299 552888 R Profilin, actin- and phosphatidylinositol 4,5-bisphosphate-binding protein, involved in cytoskeleton organization, required for normal timing of actin polymerization in response to thermal stress; localizes to plasma membrane and cytosol; Pfy1p complement(join(552299..552666,552876..552888)) Saccharomyces cerevisiae 854289 NP_014766.1 CDS LEO1 NC_001147.5 553177 554571 R Leo1p; Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation complement(553177..554571) Saccharomyces cerevisiae 854290 NP_014767.1 CDS UBP2 NC_001147.5 554825 558643 R Ubp2p; Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins, cleaves at the C terminus of ubiquitin fusions; capable of cleaving polyubiquitin and possesses isopeptidase activity complement(554825..558643) Saccharomyces cerevisiae 854291 NP_014768.2 CDS CAT5 NC_001147.5 559031 559732 R Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation; Cat5p complement(559031..559732) Saccharomyces cerevisiae 854292 NP_014769.1 CDS IAH1 NC_001147.5 559962 560678 R Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing; Iah1p complement(559962..560678) Saccharomyces cerevisiae 854293 NP_014770.1 CDS RGA1 NC_001147.5 561171 564194 D Rga1p; GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth 561171..564194 Saccharomyces cerevisiae 854294 NP_014771.1 CDS ADE2 NC_001147.5 564477 566192 R Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine; Ade2p complement(564477..566192) Saccharomyces cerevisiae 854295 NP_014772.1 CDS YOR129C NC_001147.5 566878 569559 R Yor129cp; Putative component of the outer plaque of the spindle pole body; may be involved in cation homeostasis or multidrug resistance complement(566878..569559) Saccharomyces cerevisiae 854296 NP_014773.1 CDS ORT1 NC_001147.5 569930 570808 R Ort1p; Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome complement(569930..570808) Saccharomyces cerevisiae 854297 NP_014774.1 CDS YOR131C NC_001147.5 572183 572839 R Yor131cp; Putative protein of unknown function; non-essential gene complement(572183..572839) Saccharomyces cerevisiae 854299 NP_014775.1 CDS VPS17 NC_001147.5 573176 574831 D Vps17p; Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation 573176..574831 Saccharomyces cerevisiae 854300 NP_014776.1 CDS EFT1 NC_001147.5 575099 577627 D Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; Eft1p 575099..577627 Saccharomyces cerevisiae 854301 NP_014777.1 CDS BAG7 NC_001147.5 578565 579794 D Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p; Bag7p 578565..579794 Saccharomyces cerevisiae 854302 NP_014779.1 CDS IDH2 NC_001147.5 580251 581360 D Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated; Idh2p 580251..581360 Saccharomyces cerevisiae 854303 NP_014780.1 CDS SIA1 NC_001147.5 581814 583682 R Sia1p; Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose complement(581814..583682) Saccharomyces cerevisiae 854305 NP_014781.1 CDS RUP1 NC_001147.5 584310 586325 R Rup1p; Protein involved in regulation of Rsp5p, which is an essential HECT ubiquitin ligase; required for binding of Rsp5p to Ubp2p; contains an UBA domain complement(584310..586325) Saccharomyces cerevisiae 854306 NP_014783.1 CDS SFL1 NC_001147.5 586982 589282 D Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; Sfl1p 586982..589282 Saccharomyces cerevisiae 854307 NP_014784.1 CDS ARP8 NC_001147.5 589943 592588 R Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; Arp8p complement(589943..592588) Saccharomyces cerevisiae 854309 NP_014785.1 CDS LSC1 NC_001147.5 593058 594047 D Lsc1p; Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated 593058..594047 Saccharomyces cerevisiae 854310 NP_058183.1 CDS YOR142W-B NC_001147.5 595113 600381 D Yor142w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(595113..596417,596419..600381) Saccharomyces cerevisiae 854313 NP_058184.1 CDS YOR142W-A NC_001147.5 595113 596435 D Yor142w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 595113..596435 Saccharomyces cerevisiae 854312 NP_014786.1 CDS THI80 NC_001147.5 601384 602343 R Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate); Thi80p complement(601384..602343) Saccharomyces cerevisiae 854314 NP_014787.1 CDS ELG1 NC_001147.5 602718 605093 R Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; involved in homologous recombination-mediated DNA repair; Elg1p complement(602718..605093) Saccharomyces cerevisiae 854315 NP_014788.1 CDS PNO1 NC_001147.5 605348 606172 R Pno1p; Essential nucleolar protein required for pre-18S rRNA processing, interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain complement(605348..606172) Saccharomyces cerevisiae 854317 NP_014790.2 CDS MDM32 NC_001147.5 606608 608476 D Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; Mdm32p 606608..608476 Saccharomyces cerevisiae 854318 NP_014791.1 CDS SPP2 NC_001147.5 608641 609198 R Spp2p; Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction complement(608641..609198) Saccharomyces cerevisiae 854319 NP_014792.1 CDS SMP3 NC_001147.5 609839 611389 R Smp3p; Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure complement(609839..611389) Saccharomyces cerevisiae 854320 NP_014793.1 CDS MRPL23 NC_001147.5 612000 612491 D Mrpl23p; Mitochondrial ribosomal protein of the large subunit 612000..612491 Saccharomyces cerevisiae 854321 NP_014794.1 CDS RPB2 NC_001147.5 612998 616672 R Rpb2p; RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit complement(612998..616672) Saccharomyces cerevisiae 854322 NP_014795.1 CDS YOR152C NC_001147.5 617519 618289 R Yor152cp; Putative protein of unknown function; has no similarity to any known protein; YOR152C is not an essential gene complement(617519..618289) Saccharomyces cerevisiae 854323 NP_014796.1 CDS PDR5 NC_001147.5 619841 624376 D Plasma membrane ATP-binding cassette (ABC) transporter, short-lived multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; Pdr5p 619841..624376 Saccharomyces cerevisiae 854324 NP_014797.1 CDS SLP1 NC_001147.5 624730 626493 D Slp1p; Integral membrane protein of unknown function; member of the SUN-like family of proteins 624730..626493 Saccharomyces cerevisiae 854325 NP_014798.1 CDS ISN1 NC_001147.5 626629 627981 R Isn1p; Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms complement(626629..627981) Saccharomyces cerevisiae 854326 NP_014799.1 CDS NFI1 NC_001147.5 628361 630541 R SUMO ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; involved in maintenance of proper telomere length; Nfi1p complement(628361..630541) Saccharomyces cerevisiae 854327 NP_014800.1 CDS PUP1 NC_001147.5 630967 631752 R Pup1p; Endopeptidase with trypsin-like activity that cleaves after basic residues; beta-type subunit of 20S proteasome synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z complement(630967..631752) Saccharomyces cerevisiae 854328 NP_014801.1 CDS PET123 NC_001147.5 632165 633121 D Pet123p; Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator 632165..633121 Saccharomyces cerevisiae 854329 NP_014802.1 CDS SME1 NC_001147.5 633283 633567 R Sme1p; Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E complement(633283..633567) Saccharomyces cerevisiae 854330 NP_014803.1 CDS MTR10 NC_001147.5 633840 636758 D Nuclear import receptor, mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; Mtr10p 633840..636758 Saccharomyces cerevisiae 854331 NP_014804.1 CDS PNS1 NC_001147.5 636940 638559 R Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport; Pns1p complement(636940..638559) Saccharomyces cerevisiae 854332 NP_878172.1 CDS YOR161C-C NC_001147.5 639122 639268 R Yor161c-cp; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(639122..639268) Saccharomyces cerevisiae 1466484 NP_014805.1 CDS YRR1 NC_001147.5 639561 641993 R Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; Yrr1p complement(639561..641993) Saccharomyces cerevisiae 854333 NP_014806.1 CDS DDP1 NC_001147.5 642742 643308 D Diadenosine polyphosphate hydrolase, member of the MutT family of nucleotide hydrolases with high specificity for diadenosine hexa- and pentaphosphates, required for efficient hydrolysis of diphosphorylated inositol polyphosphates; Ddp1p 642742..643308 Saccharomyces cerevisiae 854334 NP_014807.1 CDS YOR164C NC_001147.5 643395 644333 R Yor164cp; Protein of unknown function; interacts with Mdy2p complement(643395..644333) Saccharomyces cerevisiae 854335 NP_014808.1 CDS SEY1 NC_001147.5 644567 646897 D Sey1p; Protein of unknown function, contains two predicted GTP-binding motifs GXXXXGKS and DXXG near the N-terminus, homolog of the Arabidopsis gene RHD3 (Root Hair Defective) 644567..646897 Saccharomyces cerevisiae 854336 NP_014809.1 CDS SWT1 NC_001147.5 647127 648503 R Swt1p; Protein of unknown function; involved in transcription associated process; interacts genetically and physically with the TREX complex and RNA polymerase II; contains a PINc domain (PilT N terminus); localizes in the nucleus complement(647127..648503) Saccharomyces cerevisiae 854337 NP_014810.1 CDS RPS28A NC_001147.5 648805 649008 R Rps28ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Bp and has similarity to rat S28 ribosomal protein complement(648805..649008) Saccharomyces cerevisiae 854338 NP_014811.1 CDS GLN4 NC_001147.5 649304 651733 D Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions; Gln4p 649304..651733 Saccharomyces cerevisiae 854339 NP_014814.1 CDS LCB4 NC_001147.5 652011 653885 R Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; Lcb4p complement(652011..653885) Saccharomyces cerevisiae 854342 NP_014815.1 CDS YRM1 NC_001147.5 654211 656571 D Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes; Yrm1p 654211..656571 Saccharomyces cerevisiae 854343 NP_014816.2 CDS DCS2 NC_001147.5 657265 658326 D Non-essential, stress induced regulatory protein containing a HIT (histidine triad) motif; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway, similar to Dcs1p.; Dcs2p 657265..658326 Saccharomyces cerevisiae 854344 NP_014817.1 CDS MED4 NC_001147.5 658748 659602 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med4p 658748..659602 Saccharomyces cerevisiae 854345 NP_014818.1 CDS ALE1 NC_001147.5 659816 661675 R Lysophospholipid acyltransferase, partially redundant with Slc1p; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids; Ale1p complement(659816..661675) Saccharomyces cerevisiae 854346 NP_014819.1 CDS HEM15 NC_001147.5 662402 663583 D Hem15p; Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway 662402..663583 Saccharomyces cerevisiae 854347 NP_014820.1 CDS MPC54 NC_001147.5 665786 667180 R Mpc54p; Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate complement(665786..667180) Saccharomyces cerevisiae 854349 NP_014821.1 CDS GAC1 NC_001147.5 667861 670242 R Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; Gac1p complement(667861..670242) Saccharomyces cerevisiae 854350 NP_014822.1 CDS SYC1 NC_001147.5 671846 672412 R Syc1p; Subunit of the APT subcomplex of cleavage and polyadenylation factor, may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs complement(671846..672412) Saccharomyces cerevisiae 854351 NP_014823.1 CDS DCI1 NC_001147.5 674353 675168 R Peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, involved in fatty acid metabolism, contains peroxisome targeting signals at amino and carboxy termini; Dci1p complement(674353..675168) Saccharomyces cerevisiae 854352 NP_014824.1 CDS LAS17 NC_001147.5 675940 677841 D Las17p; Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) 675940..677841 Saccharomyces cerevisiae 854353 NP_014825.1 CDS RPS30B NC_001147.5 678192 678794 R Rps30bp; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Ap and has similarity to rat S30 ribosomal protein complement(join(678192..678380,678792..678794)) Saccharomyces cerevisiae 854354 NP_014826.1 CDS FYV12 NC_001147.5 678873 679262 D Protein of unknown function, required for survival upon exposure to K1 killer toxin; Fyv12p 678873..679262 Saccharomyces cerevisiae 854355 NP_014827.1 CDS SER1 NC_001147.5 679358 680545 D Ser1p; 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p 679358..680545 Saccharomyces cerevisiae 854356 NP_014828.1 CDS GSP2 NC_001147.5 681445 682107 R Gsp2p; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability complement(681445..682107) Saccharomyces cerevisiae 854357 NP_014829.1 CDS YOR186W NC_001147.5 683112 683546 D Yor186wp; Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent 683112..683546 Saccharomyces cerevisiae 854358 NP_014830.1 CDS TUF1 NC_001147.5 684031 685344 D Tuf1p; Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans 684031..685344 Saccharomyces cerevisiae 854359 NP_014831.1 CDS MSB1 NC_001147.5 685768 689181 D Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; Msb1p 685768..689181 Saccharomyces cerevisiae 854360 NP_014832.1 CDS IES4 NC_001147.5 689625 689975 D Ies4p; Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses 689625..689975 Saccharomyces cerevisiae 854361 NP_014833.1 CDS SPR1 NC_001147.5 690696 692033 D Spr1p; Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance 690696..692033 Saccharomyces cerevisiae 854362 NP_014834.1 CDS RIS1 NC_001147.5 692476 697335 D Ris1p; Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching, contains an N-terminal domain that interacts with Sir4p and a C-terminal SNF2 domain 692476..697335 Saccharomyces cerevisiae 854363 NP_014835.1 CDS THI72 NC_001147.5 698769 700568 R Transporter of thiamine or related compound; shares sequence similarity with Thi7p; Thi72p complement(698769..700568) Saccharomyces cerevisiae 854364 NP_878173.1 CDS YOR192C-C NC_001147.5 703989 704225 R Yor192c-cp; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(703989..704225) Saccharomyces cerevisiae 1466485 NP_058185.1 CDS YOR192C-B NC_001147.5 704420 709733 R Yor192c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(704420..708439,708441..709733)) Saccharomyces cerevisiae 854365 NP_058186.1 CDS YOR192C-A NC_001147.5 708417 709733 R Yor192c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(708417..709733) Saccharomyces cerevisiae 854366 NP_014836.1 CDS PEX27 NC_001147.5 710447 711577 D Pex27p; Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p 710447..711577 Saccharomyces cerevisiae 854368 NP_014837.1 CDS TOA1 NC_001147.5 711684 712544 R Toa1p; TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA complement(711684..712544) Saccharomyces cerevisiae 854369 NP_014838.1 CDS SLK19 NC_001147.5 712867 715332 D Slk19p; Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate 712867..715332 Saccharomyces cerevisiae 854370 NP_014839.1 CDS LIP5 NC_001147.5 715594 716838 R Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase; Lip5p complement(715594..716838) Saccharomyces cerevisiae 854371 NP_014840.2 CDS MCA1 NC_001147.5 717087 718385 D Putative cysteine protease similar to mammalian caspases, involved in regulation of apoptosis upon hydrogen peroxide treatment; Mca1p 717087..718385 Saccharomyces cerevisiae 854372 NP_014841.1 CDS BFR1 NC_001147.5 718654 720066 R Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity; Bfr1p complement(718654..720066) Saccharomyces cerevisiae 854373 NP_014844.1 CDS MRM1 NC_001147.5 720471 721709 R Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA; Mrm1p complement(720471..721709) Saccharomyces cerevisiae 854376 NP_014845.1 CDS HIS3 NC_001147.5 721947 722609 D Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p; His3p 721947..722609 Saccharomyces cerevisiae 854377 NP_014847.1 CDS DED1 NC_001147.5 722912 724726 D Ded1p; ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility 722912..724726 Saccharomyces cerevisiae 854379 NP_014848.1 CDS LRC5 NC_001147.5 725565 727235 R Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays decreased frequency of mitochondrial genome loss and severe growth defect in minimal glycerol media; Lrc5p complement(725565..727235) Saccharomyces cerevisiae 854380 NP_014849.1 CDS NOC2 NC_001147.5 727513 729645 D Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors; Noc2p 727513..729645 Saccharomyces cerevisiae 854381 NP_014850.1 CDS RET1 NC_001147.5 730009 733458 R Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs; Ret1p complement(730009..733458) Saccharomyces cerevisiae 854382 NP_014851.1 CDS PTP2 NC_001147.5 733926 736178 D Ptp2p; Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus 733926..736178 Saccharomyces cerevisiae 854383 NP_014852.1 CDS NPT1 NC_001147.5 736438 737727 R Npt1p; Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus complement(736438..737727) Saccharomyces cerevisiae 854384 NP_014853.1 CDS RPB10 NC_001147.5 738321 738533 D Rpb10p; RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III 738321..738533 Saccharomyces cerevisiae 854385 NP_014854.2 CDS MGM1 NC_001147.5 738925 741570 R Mitochondrial GTPase related to dynamin, present in a complex containing Ugo1p and Fzo1p; required for normal morphology of cristae and for stability of Tim11p; homolog of human OPA1 involved in autosomal dominant optic atrophy; Mgm1p complement(738925..741570) Saccharomyces cerevisiae 854386 NP_014855.1 CDS STE4 NC_001147.5 742911 744182 D Ste4p; G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats 742911..744182 Saccharomyces cerevisiae 854387 NP_014856.1 CDS SAS5 NC_001147.5 744536 745282 R Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity; Sas5p complement(744536..745282) Saccharomyces cerevisiae 854388 NP_014857.1 CDS YOR214C NC_001147.5 745594 746304 R Yor214cp; Putative protein of unknown function; YOR214C is not an essential gene complement(745594..746304) Saccharomyces cerevisiae 854389 NP_014858.1 CDS AIM41 NC_001147.5 746726 747283 R Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays altered rate of mitochondrial loss and reduced growth rate in minimal glycerol media; Aim41p complement(746726..747283) Saccharomyces cerevisiae 854390 NP_014859.1 CDS RUD3 NC_001147.5 747526 748980 R Rud3p; Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 complement(747526..748980) Saccharomyces cerevisiae 854391 NP_014860.1 CDS RFC1 NC_001147.5 749302 751887 D Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc1p 749302..751887 Saccharomyces cerevisiae 854392 NP_014862.1 CDS STE13 NC_001147.5 752215 755010 R Ste13p; Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor complement(752215..755010) Saccharomyces cerevisiae 854394 NP_014863.1 CDS RCN2 NC_001147.5 755329 756126 D Rcn2p; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS 755329..756126 Saccharomyces cerevisiae 854395 NP_014864.2 CDS MCT1 NC_001147.5 756477 757559 R Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling; Mct1p complement(756477..757559) Saccharomyces cerevisiae 854396 NP_014865.1 CDS ODC2 NC_001147.5 758331 759254 D Odc2p; Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism 758331..759254 Saccharomyces cerevisiae 854397 NP_014866.1 CDS YOR223W NC_001147.5 759783 760661 D Yor223wp; Putative protein of unknown function 759783..760661 Saccharomyces cerevisiae 854398 NP_014867.1 CDS RPB8 NC_001147.5 760826 761266 R Rpb8p; RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III complement(760826..761266) Saccharomyces cerevisiae 854399 NP_014869.1 CDS ISU2 NC_001147.5 761615 762085 R Isu2p; Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable complement(761615..762085) Saccharomyces cerevisiae 854401 NP_014870.1 CDS YOR227W NC_001147.5 762826 766566 D Yor227wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 762826..766566 Saccharomyces cerevisiae 854402 NP_014871.1 CDS YOR228C NC_001147.5 766870 767778 R Yor228cp; Protein of unknown function, localized to the mitochondrial outer membrane complement(766870..767778) Saccharomyces cerevisiae 854403 NP_014872.1 CDS WTM2 NC_001147.5 768410 769813 D Wtm2p; Transcriptional repressor involved in regulation of meiosis and silencing; contains WD repeats 768410..769813 Saccharomyces cerevisiae 854404 NP_014873.1 CDS WTM1 NC_001147.5 770801 772114 D Wtm1p; Transcriptional repressor involved in regulation of meiosis and silencing, required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats 770801..772114 Saccharomyces cerevisiae 854405 NP_014874.1 CDS MKK1 NC_001147.5 772602 774128 D Mkk1p; Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p 772602..774128 Saccharomyces cerevisiae 854406 NP_014875.1 CDS MGE1 NC_001147.5 774574 775260 D Protein of the mitochondrial matrix involved in protein import into mitochondria; acts as a cochaperone and a nucleotide release factor for Ssc1p; homolog of E. coli GrpE; Mge1p 774574..775260 Saccharomyces cerevisiae 854407 NP_014876.1 CDS KIN4 NC_001147.5 775847 778249 D Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; Kin4p 775847..778249 Saccharomyces cerevisiae 854408 NP_014877.1 CDS RPL33B NC_001147.5 778556 779406 R Rpl33bp; Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has similarity to rat L35a; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable complement(join(778556..778860,779388..779406)) Saccharomyces cerevisiae 854409 NP_014879.1 CDS DFR1 NC_001147.5 780907 781542 D Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function; Dfr1p 780907..781542 Saccharomyces cerevisiae 854411 NP_014880.1 CDS HES1 NC_001147.5 781995 783299 D Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); Hes1p 781995..783299 Saccharomyces cerevisiae 854412 NP_014881.2 CDS YOR238W NC_001147.5 783678 784589 D Yor238wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 783678..784589 Saccharomyces cerevisiae 854413 NP_014882.2 CDS ABP140 NC_001147.5 784858 786745 D Nonessential protein that binds actin filaments and localizes to actin patches and cables, has similarity to S-adenosylmethionine (AdoMet)-dependent methyltransferases; Abp140p join(784858..785688,785690..786745) Saccharomyces cerevisiae 854414 NP_014884.1 CDS MET7 NC_001147.5 786996 788642 D Met7p; Folylpolyglutamate synthetase, catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA 786996..788642 Saccharomyces cerevisiae 854415 NP_014885.1 CDS SSP2 NC_001147.5 788743 789858 R Ssp2p; Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis complement(788743..789858) Saccharomyces cerevisiae 854416 NP_014886.1 CDS PUS7 NC_001147.5 790212 792242 R Pus7p; Pseudouridine synthase, catalyzes pseudouridylation at position 35 in U2 snRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); Asp(256) mutation abolishes activity; conserved in archaea, some bacteria, and vertebrates complement(790212..792242) Saccharomyces cerevisiae 854417 NP_014887.1 CDS ESA1 NC_001147.5 792532 793869 D Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression; Esa1p 792532..793869 Saccharomyces cerevisiae 854418 NP_014888.1 CDS DGA1 NC_001147.5 794077 795333 R Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles; Dga1p complement(794077..795333) Saccharomyces cerevisiae 854419 NP_014889.1 CDS YOR246C NC_001147.5 795802 796794 R Yor246cp; Protein with similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts complement(795802..796794) Saccharomyces cerevisiae 854420 NP_014890.1 CDS SRL1 NC_001147.5 797677 798309 D Srl1p; Mannoprotein that exhibits a tight association with the cell wall, required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants 797677..798309 Saccharomyces cerevisiae 854421 NP_014892.1 CDS APC5 NC_001147.5 798675 800732 R Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; Apc5p complement(798675..800732) Saccharomyces cerevisiae 854423 NP_014893.1 CDS CLP1 NC_001147.5 800971 802308 R Subunit of cleavage factor I (CFI), involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation; Clp1p complement(800971..802308) Saccharomyces cerevisiae 854424 NP_014894.1 CDS YOR251C NC_001147.5 802551 803465 R Yor251cp; Putative protein of unknown function with similarity to human thiosulfate sulfurtransferase, an enzyme that catalyzes the transfer of sulfur from thiosulfate to cyanide, forming sulfite and thiocyanate; YOR251C is a non-essential gene complement(802551..803465) Saccharomyces cerevisiae 854425 NP_014895.2 CDS TMA16 NC_001147.5 803668 804204 D Tma16p; Protein of unknown function that associates with ribosomes 803668..804204 Saccharomyces cerevisiae 854426 NP_014896.1 CDS NAT5 NC_001147.5 804378 804908 D Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing; Nat5p 804378..804908 Saccharomyces cerevisiae 854427 NP_014897.1 CDS SEC63 NC_001147.5 805033 807024 R Sec63p; Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER complement(805033..807024) Saccharomyces cerevisiae 854428 NP_014898.1 CDS OSW1 NC_001147.5 807272 808108 D Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p; Osw1p 807272..808108 Saccharomyces cerevisiae 854429 NP_014899.1 CDS TRE2 NC_001147.5 808254 810683 R Tre2p; Protein that functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; has similarity to transferrin receptors; inviability of null mutant in systematic studies is due to proximity to CDC31 complement(808254..810683) Saccharomyces cerevisiae 854430 NP_014900.1 CDS CDC31 NC_001147.5 811008 811493 D Cdc31p; Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p 811008..811493 Saccharomyces cerevisiae 854431 NP_014901.1 CDS HNT3 NC_001147.5 811671 812324 D Member of the third branch of the histidine triad (HIT) superfamily of nucleotide-binding proteins; similar to Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; Hnt3p 811671..812324 Saccharomyces cerevisiae 854432 NP_014902.1 CDS RPT4 NC_001147.5 812395 813708 R Rpt4p; One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for spindle pole body duplication; localized mainly to the nucleus throughout the cell cycle complement(812395..813708) Saccharomyces cerevisiae 854433 NP_014903.1 CDS GCD1 NC_001147.5 813984 815720 D Gcd1p; Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression 813984..815720 Saccharomyces cerevisiae 854434 NP_014904.1 CDS RPN8 NC_001147.5 815915 816931 R Rpn8p; Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p complement(815915..816931) Saccharomyces cerevisiae 854435 NP_014905.1 CDS YOR262W NC_001147.5 817291 818334 D Yor262wp; Cytoplasmic protein of unknown function; essential gene with similarity to YLR243W; contains an ATP/GTP binding site motif 817291..818334 Saccharomyces cerevisiae 854436 NP_014907.1 CDS DSE3 NC_001147.5 818865 820157 D Dse3p; Daughter cell-specific protein, may help establish daughter fate 818865..820157 Saccharomyces cerevisiae 854437 NP_014908.1 CDS RBL2 NC_001147.5 820453 820773 D Rbl2p; Protein involved in microtubule morphogenesis, required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A 820453..820773 Saccharomyces cerevisiae 854439 NP_014909.1 CDS PNT1 NC_001147.5 821022 822293 D Pnt1p; Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine 821022..822293 Saccharomyces cerevisiae 854440 NP_014910.1 CDS HRK1 NC_001147.5 822587 824866 R Protein kinase implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; Hrk1p complement(822587..824866) Saccharomyces cerevisiae 854441 NP_014911.1 CDS YOR268C NC_001147.5 825533 825931 R Yor268cp; Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene complement(825533..825931) Saccharomyces cerevisiae 854442 NP_014912.1 CDS PAC1 NC_001147.5 826384 827868 D Protein involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; synthetic lethal with bni1; homolog of human LIS1; Pac1p 826384..827868 Saccharomyces cerevisiae 854443 NP_014913.1 CDS VPH1 NC_001147.5 828051 830573 R Vph1p; Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes complement(828051..830573) Saccharomyces cerevisiae 854444 NP_014914.1 CDS FSF1 NC_001147.5 831058 832041 R Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis; Fsf1p complement(831058..832041) Saccharomyces cerevisiae 854445 NP_014915.1 CDS YTM1 NC_001147.5 832812 834194 D Ytm1p; Constituent of 66S pre-ribosomal particles, required for maturation of the large ribosomal subunit 832812..834194 Saccharomyces cerevisiae 854446 NP_014916.1 CDS TPO4 NC_001147.5 834451 836430 R Tpo4p; Polyamine transport protein, recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane; member of the major facilitator superfamily complement(834451..836430) Saccharomyces cerevisiae 854447 NP_014917.1 CDS MOD5 NC_001147.5 837673 838959 D Mod5p; Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms 837673..838959 Saccharomyces cerevisiae 854448 NP_014918.1 CDS RIM20 NC_001147.5 839083 841068 R Rim20p; Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation complement(839083..841068) Saccharomyces cerevisiae 854449 NP_014919.1 CDS CAF20 NC_001147.5 841332 841817 D Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E; Caf20p 841332..841817 Saccharomyces cerevisiae 854450 NP_014921.1 CDS HEM4 NC_001147.5 842816 843643 D Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in the heme biosynthetic pathway; Hem4p 842816..843643 Saccharomyces cerevisiae 854452 NP_014922.1 CDS RFM1 NC_001147.5 843697 844629 R Rfm1p; DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance complement(843697..844629) Saccharomyces cerevisiae 854453 NP_014923.1 CDS FSH3 NC_001147.5 844991 845791 R Serine hydrolase; sequence is similar to Fsh1p and Fsh2p; Fsh3p complement(844991..845791) Saccharomyces cerevisiae 854454 NP_014924.1 CDS PLP2 NC_001147.5 846268 847128 R Plp2p; Essential protein with similarity to phosducins, which are G-protein regulators; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP complement(846268..847128) Saccharomyces cerevisiae 854456 NP_014926.1 CDS YOR283W NC_001147.5 847452 848144 D Yor283wp; Putative protein of unknown function with some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene 847452..848144 Saccharomyces cerevisiae 854457 NP_014927.1 CDS HUA2 NC_001147.5 848477 849208 D Hua2p; Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly 848477..849208 Saccharomyces cerevisiae 854458 NP_014928.1 CDS YOR285W NC_001147.5 849634 850053 D Yor285wp; Protein of unknown function, localized to the mitochondrial outer membrane 849634..850053 Saccharomyces cerevisiae 854459 NP_014929.1 CDS AIM42 NC_001147.5 850279 850728 D Protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss and reduced growth rate in minimal glycerol media; Aim42p 850279..850728 Saccharomyces cerevisiae 854460 NP_014930.1 CDS YOR287C NC_001147.5 850936 851838 R Yor287cp; Putative protein of unknown function; may play a role in the ribosome and rRNA biosynthesis based on expression profiles and mutant phenotype complement(850936..851838) Saccharomyces cerevisiae 854461 NP_014931.1 CDS MPD1 NC_001147.5 852120 853076 R Mpd1p; Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation complement(852120..853076) Saccharomyces cerevisiae 854462 NP_014932.1 CDS YOR289W NC_001147.5 853356 854111 D Yor289wp; Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 853356..854111 Saccharomyces cerevisiae 854463 NP_014933.1 CDS SNF2 NC_001147.5 855146 860257 R Snf2p; Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p complement(855146..860257) Saccharomyces cerevisiae 854465 NP_014934.1 CDS YOR291W NC_001147.5 861174 865592 D Yor291wp; Putative protein of unknown function; shares sequence similarity with the type V P-type ATPase Spf1p; YOR291W is not an essential protein 861174..865592 Saccharomyces cerevisiae 854466 NP_014935.1 CDS YOR292C NC_001147.5 865652 866581 R Yor292cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene complement(865652..866581) Saccharomyces cerevisiae 854467 NP_014936.1 CDS RPS10A NC_001147.5 867097 867851 D Rps10ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similarity to rat ribosomal protein S10 join(867097..867148,867586..867851) Saccharomyces cerevisiae 854468 NP_878174.1 CDS YOR293C-A NC_001147.5 867997 868146 R Yor293c-ap; Identified by expression profiling and mass spectrometry complement(867997..868146) Saccharomyces cerevisiae 1466486 NP_014937.1 CDS RRS1 NC_001147.5 868339 868950 D Rrs1p; Essential protein that binds ribosomal protein L11 and is required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; mouse homolog shows altered expression in Huntington's disease model mice 868339..868950 Saccharomyces cerevisiae 854469 NP_014938.1 CDS UAF30 NC_001147.5 869207 869893 D Uaf30p; Subunit of UAF (upstream activation factor), which is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate 869207..869893 Saccharomyces cerevisiae 854470 NP_014939.1 CDS YOR296W NC_001147.5 870201 874070 D Yor296wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene 870201..874070 Saccharomyces cerevisiae 854471 NP_014940.1 CDS TIM18 NC_001147.5 874742 875320 R Tim18p; Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane; may function to stabilize the complex complement(874742..875320) Saccharomyces cerevisiae 854472 NP_014941.1 CDS MUM3 NC_001147.5 875598 877037 D Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases; Mum3p 875598..877037 Saccharomyces cerevisiae 854473 NP_014942.2 CDS MBF1 NC_001147.5 877229 877684 R Transcriptional coactivator that bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; Mbf1p complement(877229..877684) Saccharomyces cerevisiae 854474 NP_014943.1 CDS BUD7 NC_001147.5 878433 880673 D Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; Bud7p 878433..880673 Saccharomyces cerevisiae 854475 NP_014945.1 CDS RAX1 NC_001147.5 880963 882270 D Rax1p; Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily 880963..882270 Saccharomyces cerevisiae 854477 NP_014946.1 CDS YOR302W NC_001147.5 882763 882840 D Yor302wp; CPA1 uORF , Arginine attenuator peptide, regulates translation of the CPA1 mRNA 882763..882840 Saccharomyces cerevisiae 854478 NP_014947.1 CDS CPA1 NC_001147.5 882897 884132 D Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader; Cpa1p 882897..884132 Saccharomyces cerevisiae 854479 NP_014948.1 CDS ISW2 NC_001147.5 884512 887874 D Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; Isw2p 884512..887874 Saccharomyces cerevisiae 854480 NP_014949.1 CDS YOR304C-A NC_001147.5 888518 888748 R Yor304c-ap; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck complement(888518..888748) Saccharomyces cerevisiae 854481 NP_014950.1 CDS YOR305W NC_001147.5 889020 889748 D Yor305wp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene 889020..889748 Saccharomyces cerevisiae 854482 NP_014951.1 CDS MCH5 NC_001147.5 889865 891430 R Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport; Mch5p complement(889865..891430) Saccharomyces cerevisiae 854483 NP_014952.1 CDS SLY41 NC_001147.5 892728 894089 R Sly41p; Protein involved in ER-to-Golgi transport complement(892728..894089) Saccharomyces cerevisiae 854484 NP_014953.1 CDS SNU66 NC_001147.5 894621 896384 R Snu66p; Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; has homology to human SART-1 and to an S. pombe protein; snu66 null mutation confers cold-sensitivity but is not lethal at normal growth temperatures complement(894621..896384) Saccharomyces cerevisiae 854485 NP_014955.1 CDS NOP58 NC_001147.5 896822 898357 R Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; Nop58p complement(896822..898357) Saccharomyces cerevisiae 854487 NP_014956.1 CDS HSD1 NC_001147.5 899053 899925 R Hsd1p; Endoplasmic reticulum (ER)-resident membrane protein, overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation complement(899053..899925) Saccharomyces cerevisiae 854488 NP_014957.2 CDS RPL20B NC_001147.5 900247 901191 R Rpl20bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similarity to rat L18a ribosomal protein complement(join(900247..900764,901191)) Saccharomyces cerevisiae 854489 NP_014958.1 CDS SPS4 NC_001147.5 901855 902871 R Sps4p; Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage complement(901855..902871) Saccharomyces cerevisiae 854490 NP_014960.1 CDS SFG1 NC_001147.5 904757 905797 D Sfg1p; Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate 904757..905797 Saccharomyces cerevisiae 854493 NP_014961.1 CDS COT1 NC_001147.5 906233 907552 R Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; Cot1p complement(906233..907552) Saccharomyces cerevisiae 854494 NP_878175.1 CDS YOR316C-A NC_001147.5 907723 907932 R Yor316c-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR complement(907723..907932) Saccharomyces cerevisiae 1466487 NP_014962.1 CDS FAA1 NC_001147.5 909340 911442 D Long chain fatty acyl-CoA synthetase with a preference for C12:0-C16:0 fatty acids; involved in the activation of imported fatty acids; localized to both lipid particles and mitochondrial outer membrane; essential for stationary phase; Faa1p 909340..911442 Saccharomyces cerevisiae 854495 NP_014964.1 CDS HSH49 NC_001147.5 912819 913460 D U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM); Hsh49p 912819..913460 Saccharomyces cerevisiae 854497 NP_014965.1 CDS GNT1 NC_001147.5 913616 915091 R N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus; Gnt1p complement(913616..915091) Saccharomyces cerevisiae 854498 NP_014966.1 CDS PMT3 NC_001147.5 916027 918288 D Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; target for new antifungals; Pmt3p 916027..918288 Saccharomyces cerevisiae 854499 NP_014967.1 CDS LDB19 NC_001147.5 918603 921059 R Protein of unknown function involved in maintenance of proper telomere length; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface; Ldb19p complement(918603..921059) Saccharomyces cerevisiae 854500 NP_014968.1 CDS PRO2 NC_001147.5 921532 922902 R Pro2p; Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis complement(921532..922902) Saccharomyces cerevisiae 854501 NP_014969.1 CDS FRT1 NC_001147.5 923229 925037 R Tail-anchored endoplasmic reticulum membrane protein that is a substrate of the phosphatase calcineurin, interacts with homolog Frt2p, promotes cell growth in conditions of high Na+, alkaline pH, and cell wall stress; Frt1p complement(923229..925037) Saccharomyces cerevisiae 854503 NP_014971.1 CDS MYO2 NC_001147.5 925718 930442 D One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; Myo2p 925718..930442 Saccharomyces cerevisiae 854504 NP_014972.1 CDS SNC2 NC_001147.5 930731 931078 R Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; Snc2p complement(930731..931078) Saccharomyces cerevisiae 854505 NP_014973.1 CDS PDR10 NC_001147.5 931800 936494 D Pdr10p; ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p 931800..936494 Saccharomyces cerevisiae 854506 NP_014974.1 CDS SCD5 NC_001147.5 936728 939346 R Scd5p; Protein required for normal cortical actin organization and endocytosis; multicopy suppressor of clathrin deficiency; acts as a targeting subunit for protein phosphatase type 1 complement(936728..939346) Saccharomyces cerevisiae 854507 NP_014975.2 CDS MIP1 NC_001147.5 939618 943382 R Mip1p; Catalytic subunit of the mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome. complement(939618..943382) Saccharomyces cerevisiae 854508 NP_014977.1 CDS VMA4 NC_001147.5 943653 944354 D Vma4p; Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane 943653..944354 Saccharomyces cerevisiae 854509 NP_014979.1 CDS MRS2 NC_001147.5 944593 946005 D Mrs2p; Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns 944593..946005 Saccharomyces cerevisiae 854511 NP_014980.1 CDS ALA1 NC_001147.5 946230 949106 R Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog; Ala1p complement(946230..949106) Saccharomyces cerevisiae 854513 NP_014981.1 CDS KRE5 NC_001147.5 949770 953867 D Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects; Kre5p 949770..953867 Saccharomyces cerevisiae 854514 NP_014982.1 CDS TEA1 NC_001147.5 954341 956620 D Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein; Tea1p 954341..956620 Saccharomyces cerevisiae 854515 NP_014983.1 CDS YOR338W NC_001147.5 956895 957986 D Yor338wp; Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen 956895..957986 Saccharomyces cerevisiae 854516 NP_014984.1 CDS UBC11 NC_001147.5 958359 958829 R Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2; Ubc11p complement(958359..958829) Saccharomyces cerevisiae 854517 NP_014985.1 CDS RPA43 NC_001147.5 959199 960179 R Rpa43p; RNA polymerase I subunit A43 complement(959199..960179) Saccharomyces cerevisiae 854518 NP_014986.1 CDS RPA190 NC_001147.5 960984 965978 D Rpa190p; RNA polymerase I subunit; largest subunit of RNA polymerase I 960984..965978 Saccharomyces cerevisiae 854519 NP_014987.1 CDS YOR342C NC_001147.5 966663 967622 R Yor342cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus complement(966663..967622) Saccharomyces cerevisiae 854520 NP_620389.1 CDS YOR343W-B NC_001147.5 970575 975888 D Yor343w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(970575..971868,971870..975888) Saccharomyces cerevisiae 854523 NP_620390.1 CDS YOR343W-A NC_001147.5 970575 971891 D Yor343w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 970575..971891 Saccharomyces cerevisiae 854522 NP_014989.1 CDS TYE7 NC_001147.5 977191 978066 R Tye7p; Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression complement(977191..978066) Saccharomyces cerevisiae 854525 NP_014991.1 CDS REV1 NC_001147.5 981825 984782 D Rev1p; Deoxycytidyl transferase, forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; involved in repair of abasic sites in damaged DNA 981825..984782 Saccharomyces cerevisiae 854527 NP_014992.1 CDS PYK2 NC_001147.5 984939 986459 R Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by glucose, and Pyk2p may be active under low glycolytic flux; Pyk2p complement(984939..986459) Saccharomyces cerevisiae 854529 NP_014993.1 CDS PUT4 NC_001147.5 986896 988779 R Put4p; Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells complement(986896..988779) Saccharomyces cerevisiae 854530 NP_014994.1 CDS CIN1 NC_001147.5 989786 992830 D Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; Cin1p 989786..992830 Saccharomyces cerevisiae 854531 NP_014995.1 CDS MNE1 NC_001147.5 992861 994852 R Mne1p; Mitochondrial protein similar to Lucilia illustris mitochondrial cytochrome oxidase complement(992861..994852) Saccharomyces cerevisiae 854532 NP_014996.1 CDS MEK1 NC_001147.5 995015 996508 R Mek1p; Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids complement(995015..996508) Saccharomyces cerevisiae 854533 NP_014997.1 CDS YOR352W NC_001147.5 997210 998241 D Yor352wp; Putative protein of unknown function; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus 997210..998241 Saccharomyces cerevisiae 854534 NP_014998.1 CDS SOG2 NC_001147.5 998450 1000825 R Sog2p; Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis complement(998450..1000825) Saccharomyces cerevisiae 854535 NP_014999.1 CDS MSC6 NC_001147.5 1001144 1003222 R Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Msc6p complement(1001144..1003222) Saccharomyces cerevisiae 854536 NP_015000.1 CDS GDS1 NC_001147.5 1005135 1006703 D Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Gds1p 1005135..1006703 Saccharomyces cerevisiae 854537 NP_015001.1 CDS YOR356W NC_001147.5 1007219 1009114 D Yor356wp; Mitochondrial protein with similarity to flavoprotein-type oxidoreductases; found in a large supramolecular complex with other mitochondrial dehydrogenases 1007219..1009114 Saccharomyces cerevisiae 854538 NP_015002.1 CDS SNX3 NC_001147.5 1009222 1009710 R Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p; Snx3p complement(1009222..1009710) Saccharomyces cerevisiae 854539 NP_015003.1 CDS HAP5 NC_001147.5 1010159 1010887 D Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex; Hap5p 1010159..1010887 Saccharomyces cerevisiae 854540 NP_015004.1 CDS VTS1 NC_001147.5 1011187 1012758 D Vts1p; Post-transcriptional gene regulator, RNA-binding protein containing a SAM domain; shows genetic interactions with Vti1p, which is a v-SNARE involved in cis-Golgi membrane traffic 1011187..1012758 Saccharomyces cerevisiae 854541 NP_015005.1 CDS PDE2 NC_001147.5 1013239 1014819 R Pde2p; High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon complement(1013239..1014819) Saccharomyces cerevisiae 854542 NP_015006.1 CDS PRT1 NC_001147.5 1015359 1017650 R Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; Prt1p complement(1015359..1017650) Saccharomyces cerevisiae 854543 NP_015007.1 CDS PRE10 NC_001147.5 1017878 1018744 R Pre10p; 20S proteasome alpha-type subunit complement(1017878..1018744) Saccharomyces cerevisiae 854544 NP_015008.1 CDS PIP2 NC_001147.5 1020220 1023210 R Pip2p; Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes complement(1020220..1023210) Saccharomyces cerevisiae 854545 NP_015010.1 CDS YOR365C NC_001147.5 1023459 1025570 R Yor365cp; Putative protein of unknown function; YOR365C is not an essential protein complement(1023459..1025570) Saccharomyces cerevisiae 854548 NP_015012.1 CDS SCP1 NC_001147.5 1026005 1026607 D Scp1p; Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin 1026005..1026607 Saccharomyces cerevisiae 854549 NP_015013.1 CDS RAD17 NC_001147.5 1026841 1028046 D Rad17p; Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins 1026841..1028046 Saccharomyces cerevisiae 854550 NP_015014.1 CDS RPS12 NC_001147.5 1028192 1028623 R Rps12p; Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 complement(1028192..1028623) Saccharomyces cerevisiae 854551 NP_015015.1 CDS MRS6 NC_001147.5 1029181 1030992 R Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); Mrs6p complement(1029181..1030992) Saccharomyces cerevisiae 854552 NP_015016.1 CDS GPB1 NC_001147.5 1031487 1034180 R Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb2p; Gpb1p complement(1031487..1034180) Saccharomyces cerevisiae 854553 NP_015017.1 CDS NDD1 NC_001147.5 1034805 1036469 R Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes; Ndd1p complement(1034805..1036469) Saccharomyces cerevisiae 854554 NP_015018.1 CDS NUD1 NC_001147.5 1036832 1039387 D Nud1p; Component of the spindle pole body outer plaque, required for exit from mitosis 1036832..1039387 Saccharomyces cerevisiae 854555 NP_015019.1 CDS ALD4 NC_001147.5 1039838 1041397 D Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; Ald4p 1039838..1041397 Saccharomyces cerevisiae 854556 NP_015020.1 CDS GDH1 NC_001147.5 1041676 1043040 R NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; Gdh1p complement(1041676..1043040) Saccharomyces cerevisiae 854557 NP_878176.1 CDS YOR376W-A NC_001147.5 1045194 1045349 D Yor376w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 1045194..1045349 Saccharomyces cerevisiae 1466488 NP_015022.1 CDS ATF1 NC_001147.5 1046224 1047801 D Atf1p; Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation 1046224..1047801 Saccharomyces cerevisiae 854559 NP_015023.1 CDS YOR378W NC_001147.5 1049509 1051056 D Yor378wp; Putative protein of unknown function; belongs to the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene 1049509..1051056 Saccharomyces cerevisiae 854560 NP_015025.1 CDS RDR1 NC_001147.5 1051288 1052928 D Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins; Rdr1p 1051288..1052928 Saccharomyces cerevisiae 854562 NP_015026.1 CDS FRE3 NC_001147.5 1055543 1057678 D Fre3p; Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels 1055543..1057678 Saccharomyces cerevisiae 854563 NP_878177.1 CDS YOR381W-A NC_001147.5 1058421 1058588 D Yor381w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 1058421..1058588 Saccharomyces cerevisiae 1466489 NP_015027.1 CDS FIT2 NC_001147.5 1059529 1059990 D Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall; Fit2p 1059529..1059990 Saccharomyces cerevisiae 854564 NP_015028.1 CDS FIT3 NC_001147.5 1060439 1061053 R Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall; Fit3p complement(1060439..1061053) Saccharomyces cerevisiae 854565 NP_015029.1 CDS FRE5 NC_001147.5 1061562 1063646 D Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fre5p 1061562..1063646 Saccharomyces cerevisiae 854566 NP_015030.1 CDS YOR385W NC_001147.5 1065041 1065913 D Yor385wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene 1065041..1065913 Saccharomyces cerevisiae 854567 NP_015031.1 CDS PHR1 NC_001147.5 1066837 1068534 D Phr1p; DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p 1066837..1068534 Saccharomyces cerevisiae 854568 NP_015032.1 CDS YOR387C NC_001147.5 1069619 1070239 R Yor387cp; Putative protein of unknown function; regulated by the metal-responsive Aft1p transcription factor; highly inducible in zinc-depleted conditions; localizes to the soluble fraction complement(1069619..1070239) Saccharomyces cerevisiae 854569 NP_015033.1 CDS FDH1 NC_001147.5 1071791 1072921 R NAD(+)-dependent formate dehydrogenase, may protect cells from exogenous formate; Fdh1p complement(1071791..1072921) Saccharomyces cerevisiae 854570 NP_015034.1 CDS YOR389W NC_001147.5 1074211 1076085 D Yor389wp; Putative protein of unknown function; expression regulated by copper levels 1074211..1076085 Saccharomyces cerevisiae 854571 NP_015035.1 CDS YOR390W NC_001147.5 1076782 1077909 D Yor390wp; Putative protein of unknown function 1076782..1077909 Saccharomyces cerevisiae 854572 NP_015036.1 CDS HSP33 NC_001147.5 1078543 1079256 R Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; Hsp33p complement(1078543..1079256) Saccharomyces cerevisiae 854573 NP_015038.1 CDS ERR1 NC_001147.5 1080274 1081587 D Protein of unknown function, has similarity to enolases; Err1p 1080274..1081587 Saccharomyces cerevisiae 854575 NP_015039.1 CDS PAU21 NC_001147.5 1082716 1083210 D Pau21p 1082716..1083210 Saccharomyces cerevisiae 854576 NP_878178.1 CDS YOR394C-A NC_001147.5 1084200 1084367 R Yor394c-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(1084200..1084367) Saccharomyces cerevisiae 1466490 NP_061495.2 CDS YRF1-8 NC_001147.5 1085471 1090861 D Yrf1-8p; One of several telomeric Y' element-encoded DNA helicases, known as Y'-Help1 (Y'-HELicase Protein 1) 1085471..1090861 Saccharomyces cerevisiae 854577 NP_015040.1 CDS YRF1-7 NC_001148.3 280 6007 R Yrf1-7p; Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p complement(join(280..5840,5989..6007)) Saccharomyces cerevisiae 855846 NP_015041.1 CDS PAU22 NC_001148.3 7933 8427 R Pau22p complement(7933..8427) Saccharomyces cerevisiae 855847 NP_015042.1 CDS ERR2 NC_001148.3 9557 10870 R Protein of unknown function, has similarity to enolases; Err2p complement(9557..10870) Saccharomyces cerevisiae 855848 NP_015043.1 CDS HSP32 NC_001148.3 11887 12600 D Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; Hsp32p 11887..12600 Saccharomyces cerevisiae 855849 NP_015044.1 CDS YPL279C NC_001148.3 13228 14355 R Ypl279cp; Putative protein of unknown function complement(13228..14355) Saccharomyces cerevisiae 855850 NP_015045.1 CDS YPL278C NC_001148.3 15053 15355 R Ypl278cp; Putative protein of unknown function; gene expression regulated by copper levels complement(15053..15355) Saccharomyces cerevisiae 855851 NP_015046.1 CDS YPL277C NC_001148.3 15405 16868 R Ypl277cp; Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels complement(15405..16868) Saccharomyces cerevisiae 855852 NP_015049.1 CDS SAM3 NC_001148.3 22938 24701 D Sam3p; High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p 22938..24701 Saccharomyces cerevisiae 855854 NP_015050.1 CDS SAM4 NC_001148.3 25087 26064 D Sam4p; S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio 25087..26064 Saccharomyces cerevisiae 855855 NP_015051.1 CDS YPL272C NC_001148.3 26611 28164 R Ypl272cp; Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene complement(26611..28164) Saccharomyces cerevisiae 855856 NP_015052.1 CDS ATP15 NC_001148.3 30079 30267 D Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated; Atp15p 30079..30267 Saccharomyces cerevisiae 855857 NP_015053.2 CDS MDL2 NC_001148.3 30482 32803 D Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter; Mdl2p 30482..32803 Saccharomyces cerevisiae 855858 NP_015054.1 CDS KAR9 NC_001148.3 33013 34947 D Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase; Kar9p 33013..34947 Saccharomyces cerevisiae 855859 NP_015055.1 CDS PLC1 NC_001148.3 35236 37845 D Plc1p; Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinetochore function and pseudohyphal differentiation 35236..37845 Saccharomyces cerevisiae 855860 NP_015056.1 CDS ACM1 NC_001148.3 38169 38798 D Cell cycle regulated protein of unknown function; associated with Cdh1p and may supress the APC/C[Cdh1]-mediated proteolysis of mitotic cyclins; Acm1p 38169..38798 Saccharomyces cerevisiae 855861 NP_015057.1 CDS DIM1 NC_001148.3 39121 40077 D Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing; Dim1p 39121..40077 Saccharomyces cerevisiae 855862 NP_015058.1 CDS DIP5 NC_001148.3 41043 42869 D Dip5p; Dicarboxylic amino acid permease, mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly 41043..42869 Saccharomyces cerevisiae 855863 NP_015059.1 CDS YPL264C NC_001148.3 43283 44344 R Ypl264cp; Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene complement(43283..44344) Saccharomyces cerevisiae 855864 NP_015060.1 CDS KEL3 NC_001148.3 44551 46506 R Kel3p; Cytoplasmic protein of unknown function complement(44551..46506) Saccharomyces cerevisiae 855865 NP_015061.1 CDS FUM1 NC_001148.3 47336 48802 D Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial localization determined by the N-terminal mitochondrial targeting sequence and protein conformation; phosphorylated in mitochondria; Fum1p 47336..48802 Saccharomyces cerevisiae 855866 NP_015063.1 CDS YPL260W NC_001148.3 49303 50958 D Ypl260wp; Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene 49303..50958 Saccharomyces cerevisiae 855867 NP_015064.1 CDS APM1 NC_001148.3 51244 52671 R Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting; Apm1p complement(51244..52671) Saccharomyces cerevisiae 855869 NP_015065.1 CDS THI21 NC_001148.3 53498 55153 R Thi21p; Hydroxymethylpyrimidine phosphate kinase, involved in the last steps in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p complement(53498..55153) Saccharomyces cerevisiae 855870 NP_058189.1 CDS YPL257W-B NC_001148.3 56748 62016 D Ypl257w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(56748..58052,58054..62016) Saccharomyces cerevisiae 855817 NP_058190.1 CDS YPL257W-A NC_001148.3 56748 58070 D Ypl257w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 56748..58070 Saccharomyces cerevisiae 855816 NP_015066.1 CDS YPL257W NC_001148.3 63279 63860 D Ypl257wp; Putative protein of unknown function;; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene 63279..63860 Saccharomyces cerevisiae 855818 NP_015067.1 CDS CLN2 NC_001148.3 64977 66614 R G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); Cln2p complement(64977..66614) Saccharomyces cerevisiae 855819 NP_015068.1 CDS BBP1 NC_001148.3 67725 68882 D Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p; Bbp1p 67725..68882 Saccharomyces cerevisiae 855820 NP_015069.1 CDS HFI1 NC_001148.3 69485 70951 D Hfi1p; Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions 69485..70951 Saccharomyces cerevisiae 855821 NP_015070.1 CDS VIK1 NC_001148.3 71063 73006 R Vik1p; Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p complement(71063..73006) Saccharomyces cerevisiae 855822 NP_015071.1 CDS YAH1 NC_001148.3 73363 73881 R Yah1p; Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin complement(73363..73881) Saccharomyces cerevisiae 855824 NP_015073.1 CDS ICY2 NC_001148.3 74309 74719 R Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; Icy2p complement(74309..74719) Saccharomyces cerevisiae 855825 NP_015074.1 CDS RPL36B NC_001148.3 75699 76239 R Rpl36bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Ap and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA complement(join(75699..75985,76224..76239)) Saccharomyces cerevisiae 855826 NP_015075.1 CDS GYP5 NC_001148.3 76669 79353 R Gyp5p; GTPase-activating protein (GAP) for yeast Rab family members, involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p complement(76669..79353) Saccharomyces cerevisiae 855827 NP_015076.1 CDS GAL4 NC_001148.3 79711 82356 R Gal4p; DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p complement(79711..82356) Saccharomyces cerevisiae 855828 NP_015077.1 CDS YPL247C NC_001148.3 82625 84196 R Ypl247cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; YPL247C is not an essential gene complement(82625..84196) Saccharomyces cerevisiae 855829 NP_015078.1 CDS RBD2 NC_001148.3 84509 85297 R Rbd2p; Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p complement(84509..85297) Saccharomyces cerevisiae 855830 NP_015079.1 CDS YPL245W NC_001148.3 85586 86950 D Ypl245wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm 85586..86950 Saccharomyces cerevisiae 855831 NP_015080.1 CDS HUT1 NC_001148.3 87014 88033 R Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1; Hut1p complement(87014..88033) Saccharomyces cerevisiae 855832 NP_015081.1 CDS SRP68 NC_001148.3 88517 90316 D Srp68p; Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane 88517..90316 Saccharomyces cerevisiae 855833 NP_015082.1 CDS IQG1 NC_001148.3 90622 95109 R Iqg1p; Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family complement(90622..95109) Saccharomyces cerevisiae 855834 NP_015083.1 CDS CIN2 NC_001148.3 95372 96258 R Tubulin folding factor C (putative) involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; Cin2p complement(join(95372..96153,96234..96258)) Saccharomyces cerevisiae 855835 NP_015084.1 CDS HSP82 NC_001148.3 96496 98625 R Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p; Hsp82p complement(96496..98625) Saccharomyces cerevisiae 855836 NP_015085.1 CDS YAR1 NC_001148.3 99484 100086 D Yar1p; Cytoplasmic ankyrin-repeat containing protein of unknown function, proposed to link the processes of 40S ribosomal subunit biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock 99484..100086 Saccharomyces cerevisiae 855837 NP_015087.1 CDS SUI3 NC_001148.3 100496 101353 D Sui3p; Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding 100496..101353 Saccharomyces cerevisiae 855838 NP_015088.1 CDS YPL236C NC_001148.3 101608 102702 R Ypl236cp; Putative protein kinase that exhibits Akr1p-dependent palmitoylation complement(101608..102702) Saccharomyces cerevisiae 855840 NP_015089.1 CDS RVB2 NC_001148.3 103232 104647 D Rvb2p; Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family 103232..104647 Saccharomyces cerevisiae 855841 NP_015090.1 CDS TFP3 NC_001148.3 104946 105440 R Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen; Tfp3p complement(104946..105440) Saccharomyces cerevisiae 855842 NP_015091.1 CDS NSL1 NC_001148.3 106172 106822 D Nsl1p; Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation 106172..106822 Saccharomyces cerevisiae 855843 NP_015092.1 CDS SSO1 NC_001148.3 107275 108147 D Sso1p; Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p 107275..108147 Saccharomyces cerevisiae 855844 NP_015093.1 CDS FAS2 NC_001148.3 108652 114315 D Fas2p; Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities; phosphorylated 108652..114315 Saccharomyces cerevisiae 855845 NP_015094.1 CDS USV1 NC_001148.3 115312 116487 D Usv1p; Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in protein folding, ATP binding, and cell wall biosynthesis 115312..116487 Saccharomyces cerevisiae 855871 NP_015095.1 CDS YPL229W NC_001148.3 117067 117687 D Ypl229wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL229W is not an essential gene 117067..117687 Saccharomyces cerevisiae 855872 NP_015096.1 CDS CET1 NC_001148.3 118382 120031 D Cet1p; Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 118382..120031 Saccharomyces cerevisiae 855873 NP_015097.1 CDS ALG5 NC_001148.3 120163 121167 R UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum; Alg5p complement(120163..121167) Saccharomyces cerevisiae 855874 NP_015098.1 CDS NEW1 NC_001148.3 121767 125357 D New1p; ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein 121767..125357 Saccharomyces cerevisiae 855875 NP_015099.1 CDS YPL225W NC_001148.3 126006 126446 D Ypl225wp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 126006..126446 Saccharomyces cerevisiae 855876 NP_015100.1 CDS MMT2 NC_001148.3 126738 128087 R Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p; Mmt2p complement(126738..128087) Saccharomyces cerevisiae 855877 NP_015101.1 CDS GRE1 NC_001148.3 128631 129137 R Hydrophilin of unknown function; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; Gre1p complement(128631..129137) Saccharomyces cerevisiae 855878 NP_015102.1 CDS FMP40 NC_001148.3 130161 132227 D Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp40p 130161..132227 Saccharomyces cerevisiae 855879 NP_015103.1 CDS FLC1 NC_001148.3 133042 135423 D Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; Flc1p 133042..135423 Saccharomyces cerevisiae 855880 NP_015104.1 CDS RPL1A NC_001148.3 135789 136442 D Rpl1ap; N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal 135789..136442 Saccharomyces cerevisiae 855881 NP_015105.1 CDS PCL8 NC_001148.3 136749 138227 D Pcl8p; Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation 136749..138227 Saccharomyces cerevisiae 855882 NP_015106.1 CDS SAR1 NC_001148.3 138697 139408 D Sar1p; GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport join(138697..138724,138864..139408) Saccharomyces cerevisiae 855883 NP_015107.1 CDS BMS1 NC_001148.3 139619 143170 R Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p; Bms1p complement(139619..143170) Saccharomyces cerevisiae 855884 NP_015108.1 CDS YPL216W NC_001148.3 143820 147128 D Ypl216wp; Putative protein of unknown function; YPL216W is not an essential gene 143820..147128 Saccharomyces cerevisiae 855885 NP_015109.1 CDS CBP3 NC_001148.3 147415 148422 D Mitochondrial protein required for assembly of ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); interacts with Cbp4p and function is partially redundant with that of Cbp4p; Cbp3p 147415..148422 Saccharomyces cerevisiae 855886 NP_015110.1 CDS THI6 NC_001148.3 148568 150190 R Thi6p; Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern complement(148568..150190) Saccharomyces cerevisiae 855887 NP_015111.1 CDS LEA1 NC_001148.3 150613 151329 D Lea1p; Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; invovled in telomere maintenance; putative homolog of human U2A' snRNP protein 150613..151329 Saccharomyces cerevisiae 855888 NP_015112.1 CDS PUS1 NC_001148.3 151514 153148 R tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA; Pus1p complement(151514..153148) Saccharomyces cerevisiae 855889 NP_015113.1 CDS NIP7 NC_001148.3 153494 154039 D Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p; Nip7p 153494..154039 Saccharomyces cerevisiae 855890 NP_015114.2 CDS SRP72 NC_001148.3 154289 156211 R Srp72p; Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane complement(154289..156211) Saccharomyces cerevisiae 855891 NP_015115.1 CDS IPL1 NC_001148.3 156489 157592 R Ipl1p; Aurora kinase involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; associates with Sli15p, which promotes Ipl1p association with mitotic spindle complement(156489..157592) Saccharomyces cerevisiae 855892 NP_015116.1 CDS RKM1 NC_001148.3 157841 159592 D SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit protein L23a (RPL23A and RPL23B); Rkm1p 157841..159592 Saccharomyces cerevisiae 855893 NP_015117.1 CDS TYW1 NC_001148.3 159908 162340 D Tyw1p; Protein required for the synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions 159908..162340 Saccharomyces cerevisiae 855894 NP_015118.1 CDS YPL206C NC_001148.3 162631 163596 R Ypl206cp; Protein that plays a role in metabolism of glycerophosphocholine to phosphatidylcholine; contains glycerophosphodiester phosphodiesterase motifs complement(162631..163596) Saccharomyces cerevisiae 855895 NP_015120.1 CDS HRR25 NC_001148.3 164275 165759 D Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta); Hrr25p 164275..165759 Saccharomyces cerevisiae 855897 NP_015121.1 CDS TPK2 NC_001148.3 166255 167397 D cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk1p and Tpk3p; Tpk2p 166255..167397 Saccharomyces cerevisiae 855898 NP_015122.1 CDS AFT2 NC_001148.3 168087 169337 R Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; similar to Aft1p; Aft2p complement(168087..169337) Saccharomyces cerevisiae 855899 NP_015123.1 CDS YIG1 NC_001148.3 169769 171154 R Yig1p; Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol complement(169769..171154) Saccharomyces cerevisiae 855900 NP_015124.1 CDS CSM4 NC_001148.3 171483 171953 D Protein required for accurate chromosome segregation during meiosis; Csm4p 171483..171953 Saccharomyces cerevisiae 855901 NP_015125.1 CDS YPL199C NC_001148.3 172032 172754 R Ypl199cp complement(172032..172754) Saccharomyces cerevisiae 855902 NP_015126.1 CDS RPL7B NC_001148.3 173151 174701 D Rpl7bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) join(173151..173161,173571..173664,174072..174701) Saccharomyces cerevisiae 855903 NP_015128.1 CDS OXR1 NC_001148.3 175042 175863 D Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes but not in prokaryotes; Oxr1p 175042..175863 Saccharomyces cerevisiae 855905 NP_015129.1 CDS APL5 NC_001148.3 176222 179020 D Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function; Apl5p 176222..179020 Saccharomyces cerevisiae 855906 NP_015130.1 CDS DDC1 NC_001148.3 179276 181114 D Ddc1p; DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate 179276..181114 Saccharomyces cerevisiae 855907 NP_015131.1 CDS RSA1 NC_001148.3 181402 182547 D Rsa1p; Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly 181402..182547 Saccharomyces cerevisiae 855908 NP_015132.1 CDS PRM3 NC_001148.3 182654 183055 R Prm3p; Pheromone-regulated protein required for karyogamy; localizes to the inner membrane of the nuclear envelope complement(182654..183055) Saccharomyces cerevisiae 855909 NP_015133.1 CDS YPL191C NC_001148.3 183596 184678 R Ypl191cp; Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm complement(183596..184678) Saccharomyces cerevisiae 855910 NP_015134.1 CDS NAB3 NC_001148.3 185316 187724 R Single stranded RNA binding protein; acidic ribonucleoprotein; required for termination of non-poly(A) transcripts and efficient splicing; interacts with Nrd1p; Nab3p complement(185316..187724) Saccharomyces cerevisiae 855911 NP_878179.1 CDS COA2 NC_001148.3 188306 188512 R Putative cytochrome oxidase assembly factor; identified by homology to Ashbya gossypii; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; Coa2p complement(188306..188512) Saccharomyces cerevisiae 1466518 NP_015135.1 CDS GUP2 NC_001148.3 189153 190982 D Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog; Gup2p 189153..190982 Saccharomyces cerevisiae 855912 NP_015136.1 CDS POS5 NC_001148.3 191405 192649 D Pos5p; Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress 191405..192649 Saccharomyces cerevisiae 855913 NP_015137.1 CDS MF(ALPHA)1 NC_001148.3 193647 194144 D Mf(alpha)1p; Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor 193647..194144 Saccharomyces cerevisiae 855914 NP_015138.1 CDS UIP4 NC_001148.3 194511 195425 R Uip4p; Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope complement(194511..195425) Saccharomyces cerevisiae 855916 NP_015140.1 CDS MRN1 NC_001148.3 195949 197787 R Putative RNA binding protein; Mrn1p complement(195949..197787) Saccharomyces cerevisiae 855917 NP_015141.1 CDS YPL183W-A NC_001148.3 199094 199375 D Ypl183w-ap; Homolog of the prokaryotic ribosomal protein L36, likely to be a mitochondrial ribosomal protein coded in the nuclear genome 199094..199375 Saccharomyces cerevisiae 855918 NP_015142.1 CDS RTT10 NC_001148.3 199494 202535 R Rtt10p; Cytoplasmic protein of unknown function, plays a role in restricting Ty1 transposition complement(199494..202535) Saccharomyces cerevisiae 855919 NP_015144.1 CDS CTI6 NC_001148.3 203420 204940 D Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain; Cti6p 203420..204940 Saccharomyces cerevisiae 855920 NP_015145.1 CDS TCO89 NC_001148.3 205247 207646 D Tco89p; Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin 205247..207646 Saccharomyces cerevisiae 855922 NP_015146.1 CDS PPQ1 NC_001148.3 208156 209805 D Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors; Ppq1p 208156..209805 Saccharomyces cerevisiae 855923 NP_015147.1 CDS CBC2 NC_001148.3 212157 212783 D Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif; Cbc2p 212157..212783 Saccharomyces cerevisiae 855925 NP_015148.1 CDS CUP9 NC_001148.3 213041 213961 R Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; Cup9p complement(213041..213961) Saccharomyces cerevisiae 855926 NP_015149.1 CDS TRE1 NC_001148.3 216011 218362 R Tre1p; Plasma membrane protein that binds to Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; function is redundant with that of Tre2p; has similarity to transferrin receptors complement(216011..218362) Saccharomyces cerevisiae 855927 NP_015150.2 CDS SPT14 NC_001148.3 218629 220087 D UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins; Spt14p join(218629..218645,218746..220087) Saccharomyces cerevisiae 855928 NP_015151.1 CDS NIP100 NC_001148.3 220166 222772 R Nip100p; Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) complement(220166..222772) Saccharomyces cerevisiae 855929 NP_015152.1 CDS MRPL40 NC_001148.3 223142 224035 D Mrpl40p; Mitochondrial ribosomal protein of the large subunit 223142..224035 Saccharomyces cerevisiae 855930 NP_015153.1 CDS COX10 NC_001148.3 224352 225740 R Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders; Cox10p complement(224352..225740) Saccharomyces cerevisiae 855931 NP_015154.1 CDS OYE3 NC_001148.3 226168 227370 R Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism; Oye3p complement(226168..227370) Saccharomyces cerevisiae 855932 NP_015155.1 CDS DAP1 NC_001148.3 228313 228771 D Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis; Dap1p 228313..228771 Saccharomyces cerevisiae 855933 NP_015156.1 CDS MEX67 NC_001148.3 229038 230837 R Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP; Mex67p complement(229038..230837) Saccharomyces cerevisiae 855934 NP_015157.1 CDS YPL168W NC_001148.3 231216 232508 D Ypl168wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated 231216..232508 Saccharomyces cerevisiae 855935 NP_015158.1 CDS REV3 NC_001148.3 232592 237106 R Rev3p; Catalytic subunit of DNA polymerase zeta, which is involved in DNA repair and translesion synthesis; required for mutagenesis induced by DNA damage complement(232592..237106) Saccharomyces cerevisiae 855936 NP_015159.1 CDS ATG29 NC_001148.3 237337 237978 D Protein specifically required for autophagy; may function in autophagosome formation at the pre-autophagosomal structure in collaboration with other autophagy proteins; Atg29p 237337..237978 Saccharomyces cerevisiae 855937 NP_015160.1 CDS SET6 NC_001148.3 237955 239076 R Set6p; Protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability complement(237955..239076) Saccharomyces cerevisiae 855938 NP_015161.1 CDS MLH3 NC_001148.3 239349 241496 R Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability; Mlh3p complement(239349..241496) Saccharomyces cerevisiae 855939 NP_015162.1 CDS SVS1 NC_001148.3 241917 242699 R Svs1p; Cell wall and vacuolar protein, required for wild-type resistance to vanadate complement(241917..242699) Saccharomyces cerevisiae 855940 NP_015163.1 CDS YPL162C NC_001148.3 243205 244026 R Ypl162cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology complement(243205..244026) Saccharomyces cerevisiae 855941 NP_015164.1 CDS BEM4 NC_001148.3 244318 246219 R Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length; Bem4p complement(244318..246219) Saccharomyces cerevisiae 855942 NP_015165.1 CDS CDC60 NC_001148.3 246989 250261 D Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA; Cdc60p 246989..250261 Saccharomyces cerevisiae 855943 NP_015166.1 CDS PET20 NC_001148.3 250906 251667 R Pet20p; Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome complement(250906..251667) Saccharomyces cerevisiae 855944 NP_015167.1 CDS AIM44 NC_001148.3 252033 254309 R Protein of unknown function; null mutant displays increased frequency of mitochondrial genome loss (petite formation) and reduced growth rate in minimal glycerol media; Aim44p complement(252033..254309) Saccharomyces cerevisiae 855945 NP_015168.1 CDS TGS1 NC_001148.3 254813 255760 D Trimethyl guanosine synthase, conserved nucleolar methyl transferase responsible for conversion of the m(7)G cap structure of snRNAs and snoRNAs to m(2,2,7)G; also required for ribosome synthesis and nucleolar morphology; Tgs1p 254813..255760 Saccharomyces cerevisiae 855946 NP_015169.1 CDS PRM4 NC_001148.3 255912 256766 R Prm4p; Pheromone-regulated protein, predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift complement(255912..256766) Saccharomyces cerevisiae 855947 NP_015170.1 CDS KIP2 NC_001148.3 257215 259335 R Kip2p; Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle complement(257215..259335) Saccharomyces cerevisiae 855948 NP_015171.1 CDS PEP4 NC_001148.3 259713 260930 R Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates; Pep4p complement(259713..260930) Saccharomyces cerevisiae 855949 NP_015172.1 CDS RAD53 NC_001148.3 261726 264191 R Rad53p; Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication complement(261726..264191) Saccharomyces cerevisiae 855950 NP_878180.1 CDS YPL152W-A NC_001148.3 264601 264699 D Ypl152w-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching 264601..264699 Saccharomyces cerevisiae 1466519 NP_015173.1 CDS RRD2 NC_001148.3 265027 266103 D Rrd2p; Activator of the phosphotyrosyl phosphatase activity of PP2A,peptidyl-prolyl cis/trans-isomerase; regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex 265027..266103 Saccharomyces cerevisiae 855951 NP_015174.1 CDS PRP46 NC_001148.3 266179 267534 R Splicing factor that is found in the Cef1p subcomplex of the spliceosome; Prp46p complement(266179..267534) Saccharomyces cerevisiae 855952 NP_015175.1 CDS YPL150W NC_001148.3 268187 270892 D Ypl150wp; Putative protein kinase of unknown cellular role 268187..270892 Saccharomyces cerevisiae 855953 NP_015176.1 CDS ATG5 NC_001148.3 271309 272193 D Conserved autophagy-related protein that undergoes conjugation with Atg12p and then associates with Atg16p to form a cytosolic complex essential for autophagosome formation; Atg5p 271309..272193 Saccharomyces cerevisiae 855954 NP_015177.2 CDS PPT2 NC_001148.3 272293 272814 R Ppt2p; Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation complement(272293..272814) Saccharomyces cerevisiae 855955 NP_015178.1 CDS PXA1 NC_001148.3 273254 275866 D Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins; Pxa1p 273254..275866 Saccharomyces cerevisiae 855956 NP_015179.1 CDS NOP53 NC_001148.3 276161 277528 R Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired; Nop53p complement(276161..277528) Saccharomyces cerevisiae 855957 NP_015180.1 CDS KES1 NC_001148.3 278394 279698 R Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; Kes1p complement(278394..279698) Saccharomyces cerevisiae 855958 NP_015181.1 CDS POC4 NC_001148.3 280479 280925 D Poc4p; Chaperone component involved in 20S proteasome assembly; forms a heterodimer with Irc25p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutant is viable, exhibits shortened telomeres 280479..280925 Saccharomyces cerevisiae 855959 NP_015182.1 CDS RPL33A NC_001148.3 282121 282969 D Rpl33ap; N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable join(282121..282139,282665..282969) Saccharomyces cerevisiae 855960 NP_015184.1 CDS YPL141C NC_001148.3 283463 286060 R Ypl141cp; Putative protein kinase; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL141C is not an essential gene complement(283463..286060) Saccharomyces cerevisiae 855962 NP_015185.1 CDS MKK2 NC_001148.3 287513 289033 R Mkk2p; Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p complement(287513..289033) Saccharomyces cerevisiae 855963 NP_015186.1 CDS UME1 NC_001148.3 289668 291050 R Ume1p; Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p complement(289668..291050) Saccharomyces cerevisiae 855964 NP_015187.1 CDS SPP1 NC_001148.3 291365 292426 R Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; Spp1p complement(291365..292426) Saccharomyces cerevisiae 855965 NP_015188.1 CDS GIP3 NC_001148.3 292816 296646 R Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; Gip3p complement(292816..296646) Saccharomyces cerevisiae 855967 NP_015190.1 CDS ISU1 NC_001148.3 297552 298049 D Isu1p; Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable 297552..298049 Saccharomyces cerevisiae 855968 NP_015191.1 CDS ODC1 NC_001148.3 298570 299502 R Odc1p; Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation complement(298570..299502) Saccharomyces cerevisiae 855969 NP_015192.1 CDS RDS2 NC_001148.3 299887 301227 R Zinc cluster transcriptional activator involved in conferring resistance to ketoconazole; Rds2p complement(299887..301227) Saccharomyces cerevisiae 855970 NP_015193.1 CDS COX11 NC_001148.3 301715 302617 D Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p; Cox11p 301715..302617 Saccharomyces cerevisiae 855971 NP_015194.1 CDS RPL5 NC_001148.3 303120 304013 D Rpl5p; Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly 303120..304013 Saccharomyces cerevisiae 855972 NP_015195.1 CDS SPO19 NC_001148.3 304386 305057 D Spo19p; Meiosis-specific protein of unknown function, involved in completion of nuclear divisions; identified as a weak high-copy suppressor of the spo1-1 ts mutation; putative GPI-dependent cell-wall protein 304386..305057 Saccharomyces cerevisiae 855973 NP_015196.1 CDS TAF14 NC_001148.3 305297 306136 D Taf14p; Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes, involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain join(305297..305305,305411..306136) Saccharomyces cerevisiae 855974 NP_015197.1 CDS TBF1 NC_001148.3 306532 308220 R Telobox-containing general regulatory factor; binds to TTAGGG repeats within subtelomeric anti-silencing regions (STARs) and possibly throughout the genome and mediates their insulating capacity by blocking silent chromatin propagation; Tbf1p complement(306532..308220) Saccharomyces cerevisiae 855975 NP_015198.1 CDS HHO1 NC_001148.3 308827 309603 R Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation; Hho1p complement(308827..309603) Saccharomyces cerevisiae 855976 NP_015199.1 CDS NAN1 NC_001148.3 310209 312899 D U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA; Nan1p 310209..312899 Saccharomyces cerevisiae 855977 NP_015200.1 CDS KAP120 NC_001148.3 313387 316485 D Karyopherin with a role in the assembly or export of 60S ribosomal subunits; Kap120p 313387..316485 Saccharomyces cerevisiae 855978 NP_015201.1 CDS SPC29 NC_001148.3 316754 317515 D Spc29p; Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication 316754..317515 Saccharomyces cerevisiae 855979 NP_015202.1 CDS RNY1 NC_001148.3 317640 318944 R Rny1p; RNAse; member of the T(2) family of endoribonucleases complement(317640..318944) Saccharomyces cerevisiae 855980 NP_015203.1 CDS TFB2 NC_001148.3 319225 320766 R Tfb2p; Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH complement(319225..320766) Saccharomyces cerevisiae 855981 NP_015204.1 CDS MEI5 NC_001148.3 320961 321629 R Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p; Mei5p complement(320961..321629) Saccharomyces cerevisiae 855982 NP_015205.1 CDS VPS30 NC_001148.3 322070 323743 D Vps30p; Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; ortholog of the higher eukaryotic gene Beclin 1 322070..323743 Saccharomyces cerevisiae 855983 NP_878181.1 CDS YPL119C-A NC_001148.3 324023 324286 R Ypl119c-ap; Putative protein of unknown function; identified by expression profiling and mass spectrometry complement(324023..324286) Saccharomyces cerevisiae 1466520 NP_015206.1 CDS DBP1 NC_001148.3 324410 326263 R Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; Dbp1p complement(324410..326263) Saccharomyces cerevisiae 855984 NP_015207.1 CDS MRP51 NC_001148.3 326627 327661 D Mrp51p; Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences 326627..327661 Saccharomyces cerevisiae 855985 NP_015208.1 CDS IDI1 NC_001148.3 327863 328729 R Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; Idi1p complement(327863..328729) Saccharomyces cerevisiae 855986 NP_015209.1 CDS HOS3 NC_001148.3 329605 331698 D Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats; Hos3p 329605..331698 Saccharomyces cerevisiae 855987 NP_015210.1 CDS BEM3 NC_001148.3 332099 335485 R Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly; Bem3p complement(332099..335485) Saccharomyces cerevisiae 855988 NP_015212.1 CDS YPL113C NC_001148.3 335952 337142 R Ypl113cp; Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene complement(335952..337142) Saccharomyces cerevisiae 855990 NP_015213.1 CDS PEX25 NC_001148.3 337435 338619 R Pex25p; Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p complement(337435..338619) Saccharomyces cerevisiae 855991 NP_015214.1 CDS CAR1 NC_001148.3 339943 340944 D Car1p; Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance 339943..340944 Saccharomyces cerevisiae 855993 NP_015215.1 CDS GDE1 NC_001148.3 341067 344738 R Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes; Gde1p complement(341067..344738) Saccharomyces cerevisiae 855994 NP_015216.3 CDS YPL109C NC_001148.3 345263 347388 R Ypl109cp; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(join(345263..345443,345596..347388)) Saccharomyces cerevisiae 855995 NP_015217.1 CDS YPL108W NC_001148.3 348443 348949 D Ypl108wp; Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS 348443..348949 Saccharomyces cerevisiae 855996 NP_015218.1 CDS YPL107W NC_001148.3 349116 349862 D Ypl107wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene 349116..349862 Saccharomyces cerevisiae 855997 NP_015219.1 CDS SSE1 NC_001148.3 350191 352272 R Sse1p; ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm complement(350191..352272) Saccharomyces cerevisiae 855998 NP_015220.1 CDS YPL105C NC_001148.3 352860 355409 R Ypl105cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(352860..355409) Saccharomyces cerevisiae 855999 NP_015221.1 CDS MSD1 NC_001148.3 355697 357673 D Msd1p; Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene 355697..357673 Saccharomyces cerevisiae 856000 NP_015222.1 CDS FMP30 NC_001148.3 357997 359403 R Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Fmp30p complement(357997..359403) Saccharomyces cerevisiae 856001 NP_015224.1 CDS ELP4 NC_001148.3 360206 361576 D Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity; Elp4p 360206..361576 Saccharomyces cerevisiae 856002 NP_015225.1 CDS ATG21 NC_001148.3 361869 363359 D Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; Atg21p 361869..363359 Saccharomyces cerevisiae 856004 NP_015226.1 CDS AIM43 NC_001148.3 363517 364065 R Protein of unknown function;non-tagged protein detected in purified mitochondria in high-throughput studies; null mutant displays increased frequency of mitochondrial genome loss and severe growth defect in minimal glycerol media; Aim43p complement(363517..364065) Saccharomyces cerevisiae 856005 NP_015227.1 CDS MGR2 NC_001148.3 364383 364724 R Protein required for growth of cells lacking the mitochondrial genome; Mgr2p complement(364383..364724) Saccharomyces cerevisiae 856006 NP_015228.1 CDS MSY1 NC_001148.3 364949 366427 D Msy1p; Mitochondrial tyrosyl-tRNA synthetase 364949..366427 Saccharomyces cerevisiae 856007 NP_690846.1 CDS ERI1 NC_001148.3 366526 366732 R Eri1p; Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein complement(366526..366732) Saccharomyces cerevisiae 856008 NP_015229.1 CDS PNG1 NC_001148.3 366924 368015 D Png1p; Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation, localizes to the cytoplasm and nucleus, interacts with the DNA repair protein Rad23p 366924..368015 Saccharomyces cerevisiae 856009 NP_015230.1 CDS EEB1 NC_001148.3 368068 369438 R Eeb1p; Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short chain esterase activity; may be involved in lipid metabolism and detoxification complement(368068..369438) Saccharomyces cerevisiae 856010 NP_015231.2 CDS SEC62 NC_001148.3 369836 370660 R Sec62p; Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER complement(369836..370660) Saccharomyces cerevisiae 856011 NP_015232.1 CDS NOG1 NC_001148.3 370975 372918 D Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins; Nog1p 370975..372918 Saccharomyces cerevisiae 856012 NP_015233.1 CDS SSU1 NC_001148.3 373790 375166 D Ssu1p; Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein 373790..375166 Saccharomyces cerevisiae 856013 NP_015234.1 CDS GLR1 NC_001148.3 375499 376950 D Glr1p; Cytosolic and mitochondrial glutathione oxidoreductase, converts oxidized glutathione to reduced glutathione 375499..376950 Saccharomyces cerevisiae 856014 NP_015235.1 CDS RPS6A NC_001148.3 377288 378392 R Rps6ap; Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein complement(join(377288..377992,378387..378392)) Saccharomyces cerevisiae 856015 NP_015236.1 CDS RLM1 NC_001148.3 379117 381147 R Rlm1p; MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p complement(379117..381147) Saccharomyces cerevisiae 856016 NP_015237.1 CDS YPL088W NC_001148.3 381962 382990 D Ypl088wp; Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance 381962..382990 Saccharomyces cerevisiae 856017 NP_015238.1 CDS YDC1 NC_001148.3 383452 384405 D Ydc1p; Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity 383452..384405 Saccharomyces cerevisiae 856018 NP_015239.1 CDS ELP3 NC_001148.3 384770 386443 R Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin; Elp3p complement(384770..386443) Saccharomyces cerevisiae 856019 NP_015240.1 CDS SEC16 NC_001148.3 387064 393651 D Sec16p; COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p 387064..393651 Saccharomyces cerevisiae 856020 NP_015241.1 CDS BRO1 NC_001148.3 394035 396569 D Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes; Bro1p 394035..396569 Saccharomyces cerevisiae 856021 NP_015242.1 CDS SEN54 NC_001148.3 396699 398102 R Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen54p complement(396699..398102) Saccharomyces cerevisiae 856022 NP_015243.1 CDS MOT1 NC_001148.3 398477 404080 R Mot1p; Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity complement(398477..404080) Saccharomyces cerevisiae 856023 NP_015244.1 CDS RPS9A NC_001148.3 404947 406041 D Rps9ap; Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Bp and has similarity to E. coli S4 and rat S9 ribosomal proteins join(404947..404953,405455..406041) Saccharomyces cerevisiae 856024 NP_015246.1 CDS RPL21B NC_001148.3 406633 407536 D Rpl21bp; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat L21 ribosomal protein join(406633..406643,407065..407536) Saccharomyces cerevisiae 856026 NP_015247.1 CDS ATP4 NC_001148.3 408007 408741 R Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated; Atp4p complement(408007..408741) Saccharomyces cerevisiae 856027 NP_015248.1 CDS YPL077C NC_001148.3 409365 410087 R Ypl077cp; Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene complement(409365..410087) Saccharomyces cerevisiae 856028 NP_015249.1 CDS GPI2 NC_001148.3 410440 411282 D Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; Gpi2p 410440..411282 Saccharomyces cerevisiae 856029 NP_015250.1 CDS GCR1 NC_001148.3 412251 415359 D Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p; Gcr1p join(412251..412258,413010..415359) Saccharomyces cerevisiae 856030 NP_015251.1 CDS YTA6 NC_001148.3 415760 418024 D Yta6p; Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localized to the cortex of mother cells but not to daughter cells 415760..418024 Saccharomyces cerevisiae 856031 NP_015253.1 CDS UBP16 NC_001148.3 418509 420008 D Ubp16p; Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria 418509..420008 Saccharomyces cerevisiae 856032 NP_015254.1 CDS YPL071C NC_001148.3 420045 420515 R Ypl071cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus complement(420045..420515) Saccharomyces cerevisiae 856034 NP_015255.1 CDS MUK1 NC_001148.3 420945 422783 D Muk1p; Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation 420945..422783 Saccharomyces cerevisiae 856035 NP_015256.1 CDS BTS1 NC_001148.3 422882 423889 R Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic; Bts1p complement(422882..423889) Saccharomyces cerevisiae 856036 NP_015257.1 CDS YPL068C NC_001148.3 424212 425093 R Ypl068cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS complement(424212..425093) Saccharomyces cerevisiae 856037 NP_015258.1 CDS YPL067C NC_001148.3 425248 425844 R Ypl067cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene complement(425248..425844) Saccharomyces cerevisiae 856038 NP_015259.1 CDS YPL066W NC_001148.3 426230 427669 D Ypl066wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source 426230..427669 Saccharomyces cerevisiae 856039 NP_015260.1 CDS VPS28 NC_001148.3 427924 428652 D Vps28p; Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; involved in transport of precursors for soluble vacuolar hydrolases from the late endosome to the vacuole 427924..428652 Saccharomyces cerevisiae 856040 NP_015261.1 CDS CWC27 NC_001148.3 428708 429613 R Cwc27p; Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p complement(428708..429613) Saccharomyces cerevisiae 856041 NP_015262.1 CDS TIM50 NC_001148.3 429936 431366 D Tim50p; Constituent of the mitochondrial inner membrane presequence translocase (TIM23 complex); may promote binding of incoming precursor proteins to the intermembrane space domain of Tom22p during translocation 429936..431366 Saccharomyces cerevisiae 856042 NP_015264.1 CDS ALD6 NC_001148.3 432585 434087 D Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress; Ald6p 432585..434087 Saccharomyces cerevisiae 856044 NP_015265.1 CDS LPE10 NC_001148.3 434520 435761 D Mitochondrial inner membrane magnesium transporter, involved in maintenance of magnesium concentrations inside mitochondria; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p; Lpe10p 434520..435761 Saccharomyces cerevisiae 856045 NP_015266.1 CDS GRX5 NC_001148.3 444576 445028 D Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p; Grx5p 444576..445028 Saccharomyces cerevisiae 856048 NP_015267.1 CDS PDR12 NC_001148.3 445839 450374 R Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity; Pdr12p complement(445839..450374) Saccharomyces cerevisiae 856049 NP_015268.1 CDS SUR1 NC_001148.3 451906 453054 R Sur1p; Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p complement(451906..453054) Saccharomyces cerevisiae 856050 NP_015269.1 CDS YPL056C NC_001148.3 453427 453732 R Ypl056cp; Putative protein of unknown function; deletion mutant is fluconazole resistant complement(453427..453732) Saccharomyces cerevisiae 856051 NP_015270.1 CDS LGE1 NC_001148.3 453989 454987 R Protein of unknown function; null mutant forms abnormally large cells; Lge1p complement(453989..454987) Saccharomyces cerevisiae 856052 NP_015271.1 CDS LEE1 NC_001148.3 455756 456661 D Zinc-finger protein of unknown function; Lee1p 455756..456661 Saccharomyces cerevisiae 856053 NP_015272.1 CDS KTR6 NC_001148.3 457115 458455 R Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; Ktr6p complement(457115..458455) Saccharomyces cerevisiae 856054 NP_015273.2 CDS OAZ1 NC_001148.3 458796 459675 D Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to regulate ubiquitin-independent degradation; ribosomal frameshifting during synthesis of Oaz1p and its ubiquitin-mediated degradation are both polyamine-regulated; Oaz1p join(458796..459002,459004..459675) Saccharomyces cerevisiae 856055 NP_015274.1 CDS ARL3 NC_001148.3 459960 460556 D GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor; Arl3p 459960..460556 Saccharomyces cerevisiae 856056 NP_015275.1 CDS MNN9 NC_001148.3 460776 461963 R Subunit of Golgi mannosyltransferase complex also containing Anp1p, Mnn10p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; Mnn9p complement(460776..461963) Saccharomyces cerevisiae 856057 NP_015276.1 CDS DIG1 NC_001148.3 462478 463836 R Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription; Dig1p complement(462478..463836) Saccharomyces cerevisiae 856058 NP_015277.1 CDS CAM1 NC_001148.3 464398 465645 D Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma; Cam1p 464398..465645 Saccharomyces cerevisiae 856059 NP_015278.1 CDS SGF11 NC_001148.3 465959 466258 D Sgf11p; Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation 465959..466258 Saccharomyces cerevisiae 856060 NP_015279.1 CDS ELC1 NC_001148.3 466641 466940 R Elongin C, forms heterodimer with Ela1p that participates in transcription elongation; required for ubiquitin-dependent degradation of the RNA Polymerase II subunit RPO21; expression dramatically upregulated during sporulation; Elc1p complement(466641..466940) Saccharomyces cerevisiae 856061 NP_015280.1 CDS VPS16 NC_001148.3 467257 469653 D Vps16p; Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages 467257..469653 Saccharomyces cerevisiae 856062 NP_015282.1 CDS NOP4 NC_001148.3 469936 471993 D Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs); Nop4p 469936..471993 Saccharomyces cerevisiae 856063 NP_015283.1 CDS SSN3 NC_001148.3 473037 474704 R Ssn3p; Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression complement(473037..474704) Saccharomyces cerevisiae 856065 NP_015284.1 CDS YPL041C NC_001148.3 475115 475738 R Ypl041cp; Protein of unknown function involved in maintenance of proper telomere length complement(475115..475738) Saccharomyces cerevisiae 856066 NP_015285.1 CDS ISM1 NC_001148.3 475990 478998 R Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth; Ism1p complement(475990..478998) Saccharomyces cerevisiae 856067 NP_015286.1 CDS YPL039W NC_001148.3 479222 480172 D Ypl039wp; Putative protein of unknown function; YPL039W is not an essential gene 479222..480172 Saccharomyces cerevisiae 856068 NP_878182.1 CDS YPL038W-A NC_001148.3 480179 480370 D Ypl038w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 480179..480370 Saccharomyces cerevisiae 1466521 NP_015287.1 CDS MET31 NC_001148.3 480532 481065 D Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met32p; Met31p 480532..481065 Saccharomyces cerevisiae 856069 NP_015288.1 CDS EGD1 NC_001148.3 481425 481898 R Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; Egd1p complement(481425..481898) Saccharomyces cerevisiae 856070 NP_015289.1 CDS PMA2 NC_001148.3 482841 485684 D Pma2p; Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential 482841..485684 Saccharomyces cerevisiae 856071 NP_015291.1 CDS YPL034W NC_001148.3 486710 487207 D Ypl034wp; Putative protein of unknown function; YPL034W is not essential gene 486710..487207 Saccharomyces cerevisiae 856072 NP_015292.1 CDS YPL033C NC_001148.3 487360 488205 R Ypl033cp; Putative protein of unknown function; may be involved in DNA metabolism; expression is induced by Kar4p complement(487360..488205) Saccharomyces cerevisiae 856074 NP_015293.1 CDS SVL3 NC_001148.3 488885 491362 R Svl3p; Protein of unknown function, mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck complement(488885..491362) Saccharomyces cerevisiae 856075 NP_015294.1 CDS PHO85 NC_001148.3 492016 493035 R Pho85p; Cyclin-dependent kinase, with ten cyclin partners; involved in environmental stress response; in phosphate-rich conditions, Pho85p-Pho80p complex phosphorylates Pho4p which in turn represses PHO5 complement(join(492016..492916,493019..493035)) Saccharomyces cerevisiae 856076 NP_015295.1 CDS TRM44 NC_001148.3 493541 495244 D Trm44p; Putative tRNA U44 2'-O-methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene 493541..495244 Saccharomyces cerevisiae 856077 NP_015296.1 CDS SUV3 NC_001148.3 495504 497717 D Suv3p; ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs 495504..497717 Saccharomyces cerevisiae 856078 NP_015297.1 CDS ERG10 NC_001148.3 498094 499290 D Erg10p; Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis 498094..499290 Saccharomyces cerevisiae 856079 NP_015298.1 CDS SMA1 NC_001148.3 499663 500400 D Sma1p; Protein of unknown function involved in the assembly of the prospore membrane during sporulation 499663..500400 Saccharomyces cerevisiae 856080 NP_015299.1 CDS SKS1 NC_001148.3 500673 502181 R Sks1p; Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway complement(500673..502181) Saccharomyces cerevisiae 856081 NP_015301.1 CDS RMI1 NC_001148.3 503515 504240 D Rmi1p; Involved in response to DNA damage; null mutants have increased rates of recombination and delayed S phase; interacts physically and genetically with Sgs1p (RecQ family member) and Top3p (topoisomerase III) 503515..504240 Saccharomyces cerevisiae 856083 NP_015302.1 CDS MET12 NC_001148.3 504337 506310 R Met12p; Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway complement(504337..506310) Saccharomyces cerevisiae 856084 NP_015303.1 CDS RAD1 NC_001148.3 506695 509997 D Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein; Rad1p 506695..509997 Saccharomyces cerevisiae 856085 NP_015304.1 CDS ECM23 NC_001148.3 511099 511662 D Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p; Ecm23p 511099..511662 Saccharomyces cerevisiae 856086 NP_015305.1 CDS ULP1 NC_001148.3 512311 514176 R Ulp1p; Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates; specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase complement(512311..514176) Saccharomyces cerevisiae 856087 NP_015306.1 CDS VTC3 NC_001148.3 514509 517016 R Vtc3p; Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion complement(514509..517016) Saccharomyces cerevisiae 856088 NP_015307.1 CDS CTF19 NC_001148.3 517649 518758 D Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; Ctf19p 517649..518758 Saccharomyces cerevisiae 856089 NP_015308.1 CDS IRC15 NC_001148.3 518732 520231 R Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; null mutant displays increased levels of spontaneous Rad52 foci; Irc15p complement(518732..520231) Saccharomyces cerevisiae 856090 NP_015309.1 CDS SWI1 NC_001148.3 521011 524955 D Swi1p; Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2 521011..524955 Saccharomyces cerevisiae 856091 NP_015310.1 CDS HST2 NC_001148.3 525807 526880 R Hst2p; Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro complement(525807..526880) Saccharomyces cerevisiae 856092 NP_015311.1 CDS YPL014W NC_001148.3 527544 528689 D Ypl014wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus 527544..528689 Saccharomyces cerevisiae 856093 NP_015312.1 CDS MRPS16 NC_001148.3 528982 529347 R Mrps16p; Mitochondrial ribosomal protein of the small subunit complement(528982..529347) Saccharomyces cerevisiae 856094 NP_015313.1 CDS RRP12 NC_001148.3 529720 533406 D Rrp12p; Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; contains HEAT-repeats 529720..533406 Saccharomyces cerevisiae 856095 NP_015314.1 CDS TAF3 NC_001148.3 533639 534700 R Taf3p; TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation complement(533639..534700) Saccharomyces cerevisiae 856096 NP_015315.1 CDS RET3 NC_001148.3 535015 535584 D Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER; Ret3p 535015..535584 Saccharomyces cerevisiae 856097 NP_015316.1 CDS YPL009C NC_001148.3 535817 538933 R Ypl009cp; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL009C is not an essential gene complement(535817..538933) Saccharomyces cerevisiae 856098 NP_015317.1 CDS CHL1 NC_001148.3 539382 541967 D Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p; Chl1p 539382..541967 Saccharomyces cerevisiae 856099 NP_015318.1 CDS TFC8 NC_001148.3 542079 543845 R Tfc8p; One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 complement(542079..543845) Saccharomyces cerevisiae 856100 NP_015319.1 CDS NCR1 NC_001148.3 544628 548140 D Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein; Ncr1p 544628..548140 Saccharomyces cerevisiae 856101 NP_015320.1 CDS AEP3 NC_001148.3 548483 550303 D Peripheral mitochondrial inner membrane protein, located on the matrix face of the membrane; stabilizes the bicistronic AAP1-ATP6 mRNA encoding subunits 6 and 8 of the ATP synthase complex; Aep3p 548483..550303 Saccharomyces cerevisiae 856102 NP_015321.1 CDS LSP1 NC_001148.3 550629 551654 R Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; Lsp1p complement(550629..551654) Saccharomyces cerevisiae 856103 NP_015322.1 CDS ULA1 NC_001148.3 552017 553405 D Ula1p; Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation 552017..553405 Saccharomyces cerevisiae 856104 NP_015323.1 CDS SNF8 NC_001148.3 553624 554325 R Snf8p; Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression complement(553624..554325) Saccharomyces cerevisiae 856105 NP_015324.1 CDS HAT1 NC_001148.3 554602 555726 D Hat1p; Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair 554602..555726 Saccharomyces cerevisiae 856106 NP_015325.1 CDS CIT3 NC_001148.3 556374 557834 D Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate; Cit3p 556374..557834 Saccharomyces cerevisiae 856107 NP_015326.1 CDS PDH1 NC_001148.3 558382 559932 D Pdh1p; Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate 558382..559932 Saccharomyces cerevisiae 856108 NP_015328.1 CDS YPR003C NC_001148.3 561501 563765 R Ypr003cp; Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene complement(561501..563765) Saccharomyces cerevisiae 856111 NP_015329.1 CDS AIM45 NC_001148.3 564004 565038 R Protein with similarity to mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with yeast frataxin Yfh1p; null mutant displays increased frequency of mitochondrial genome loss (petite formation); Aim45p complement(564004..565038) Saccharomyces cerevisiae 856112 NP_015330.1 CDS HAL1 NC_001148.3 565784 566668 R Hal1p; Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p complement(565784..566668) Saccharomyces cerevisiae 856113 NP_015331.1 CDS ICL2 NC_001148.3 567266 568993 R Icl2p; 2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol complement(567266..568993) Saccharomyces cerevisiae 856114 NP_015332.1 CDS REC8 NC_001148.3 569333 571375 R Rec8p; Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p complement(569333..571375) Saccharomyces cerevisiae 856115 NP_015333.1 CDS HAA1 NC_001148.3 573015 575099 D Transcriptional activator involved in the transcription of TPO2, HSP30 and other genes encoding membrane stress proteins; involved in adaptation to weak acid stress; Haa1p 573015..575099 Saccharomyces cerevisiae 856117 NP_015334.1 CDS SUT2 NC_001148.3 576549 577355 D Sut2p; Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p 576549..577355 Saccharomyces cerevisiae 856118 NP_015335.1 CDS RPA135 NC_001148.3 577582 581193 R Rpa135p; RNA polymerase I subunit A135 complement(577582..581193) Saccharomyces cerevisiae 856119 NP_001091638.1 CDS YPR010C-A NC_001148.3 582370 582731 R Ypr010c-ap; Putative protein of unknown function; conserved among Saccharomyces sensu stricto species complement(join(582370..582555,582699..582731)) Saccharomyces cerevisiae 5142379 NP_015336.1 CDS YPR011C NC_001148.3 583059 584039 R Ypr011cp; Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies complement(583059..584039) Saccharomyces cerevisiae 856121 NP_015338.1 CDS YPR013C NC_001148.3 584629 585582 R Ypr013cp; Putative zinc finger protein; YPR013C is not an essential gene complement(584629..585582) Saccharomyces cerevisiae 856123 NP_015340.1 CDS YPR015C NC_001148.3 590280 591023 R Ypr015cp; Putative protein of unknown function complement(590280..591023) Saccharomyces cerevisiae 856125 NP_015341.1 CDS TIF6 NC_001148.3 592329 593066 R Tif6p; Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits complement(592329..593066) Saccharomyces cerevisiae 856126 NP_015342.1 CDS DSS4 NC_001148.3 593483 593914 R Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol; Dss4p complement(593483..593914) Saccharomyces cerevisiae 856128 NP_015343.1 CDS RLF2 NC_001148.3 594473 596293 D Rlf2p; Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) with Cac2p and Msi1p that assembles newly synthesized histones onto recently replicated DNA; involved in the maintenance of transcriptionally silent chromatin 594473..596293 Saccharomyces cerevisiae 856129 NP_015344.1 CDS MCM4 NC_001148.3 596747 599548 D Mcm4p; Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt the DNA prior to replication; accumulates in the nucleus in G1; homolog of S. pombe Cdc21p 596747..599548 Saccharomyces cerevisiae 856130 NP_015345.1 CDS ATP20 NC_001148.3 599867 600214 D Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex; Atp20p 599867..600214 Saccharomyces cerevisiae 856131 NP_015346.1 CDS AGC1 NC_001148.3 600646 603354 R Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis; Agc1p complement(600646..603354) Saccharomyces cerevisiae 856132 NP_015347.1 CDS YPR022C NC_001148.3 603908 607309 R Ypr022cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS complement(603908..607309) Saccharomyces cerevisiae 856133 NP_015348.1 CDS EAF3 NC_001148.3 608823 610028 R Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; Eaf3p complement(608823..610028) Saccharomyces cerevisiae 856134 NP_015349.1 CDS YME1 NC_001148.3 610478 612721 D Yme1p; Subunit, with Mgr1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; mutation causes an elevated rate of mitochondrial turnover 610478..612721 Saccharomyces cerevisiae 856135 NP_015350.1 CDS CCL1 NC_001148.3 613374 614555 R Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters; Ccl1p complement(613374..614555) Saccharomyces cerevisiae 856136 NP_015351.1 CDS ATH1 NC_001148.3 615376 619011 D Ath1p; Acid trehalase required for utilization of extracellular trehalose 615376..619011 Saccharomyces cerevisiae 856137 NP_015352.1 CDS YPR027C NC_001148.3 620422 621255 R Ypr027cp; Putative protein of unknown function complement(620422..621255) Saccharomyces cerevisiae 856138 NP_015353.1 CDS YOP1 NC_001148.3 623524 624199 D Yop1p; Membrane protein that interacts with Yip1p to mediate membrane traffic; overexpression results in cell death and accumulation of internal cell membranes; regulates vesicular traffic in stressed cells join(623524..623574,623708..624199) Saccharomyces cerevisiae 856140 NP_015354.1 CDS APL4 NC_001148.3 624466 626964 R Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport; Apl4p complement(624466..626964) Saccharomyces cerevisiae 856141 NP_015355.1 CDS CSR2 NC_001148.3 627877 631242 D Nuclear protein with a potential regulatory role in utilization of galactose and nonfermentable carbon sources; overproduction suppresses the lethality at high temperature of a chs5 spa2 double null mutation; potential Cdc28p substrate; Csr2p 627877..631242 Saccharomyces cerevisiae 856142 NP_015356.1 CDS NTO1 NC_001148.3 631512 633758 D Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; Nto1p 631512..633758 Saccharomyces cerevisiae 856143 NP_015357.1 CDS SRO7 NC_001148.3 634120 637221 D Sro7p; Putative effector of Rab GTPase Sec4p, forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro77p and Drosophila lgl tumor suppressor 634120..637221 Saccharomyces cerevisiae 856144 NP_015358.1 CDS HTS1 NC_001148.3 637376 639016 R Hts1p; Cytoplasmic and mitochondrial histidine tRNA synthetase; encoded by a single nuclear gene that specifies two messages; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence complement(637376..639016) Saccharomyces cerevisiae 856145 NP_015359.1 CDS ARP7 NC_001148.3 639522 640955 D Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation; Arp7p 639522..640955 Saccharomyces cerevisiae 856146 NP_015360.1 CDS GLN1 NC_001148.3 642205 643317 D Glutamine synthetase (GS), synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; Gln1p 642205..643317 Saccharomyces cerevisiae 856147 NP_015361.1 CDS VMA13 NC_001148.3 643833 645269 D Vma13p; Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase 643833..645269 Saccharomyces cerevisiae 856148 NP_690847.1 CDS YPR036W-A NC_001148.3 645947 646150 D Ypr036w-ap; Protein of unknown function; transcription is regulated by Pdr1p 645947..646150 Saccharomyces cerevisiae 856149 NP_015362.1 CDS ERV2 NC_001148.3 646445 647035 R Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER; Erv2p complement(646445..647035) Saccharomyces cerevisiae 856152 NP_015365.1 CDS TIP41 NC_001148.3 647302 648372 D Tip41p; Protein that interacts physically and genetically with Tap42p, which regulates protein phosphatase 2A; component of the TOR (target of rapamycin) signaling pathway 647302..648372 Saccharomyces cerevisiae 856153 NP_015366.1 CDS TIF5 NC_001148.3 648701 649918 D Translation initiation factor eIF-5; N-terminal domain functions as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP; C-terminal domain is the core of ribosomal preinitiation complex formation; Tif5p 648701..649918 Saccharomyces cerevisiae 856154 NP_015367.1 CDS PUF2 NC_001148.3 650432 653659 R Puf2p; Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins complement(650432..653659) Saccharomyces cerevisiae 856155 NP_015368.1 CDS RPL43A NC_001148.3 654163 654844 D Rpl43ap; Protein component of the large (60S) ribosomal subunit, identical to Rpl43Bp and has similarity to rat L37a ribosomal protein; null mutation confers a dominant lethal phenotype join(654163..654164,654568..654844) Saccharomyces cerevisiae 856156 NP_015370.1 CDS MNI2 NC_001148.3 655137 656549 R Mni2p; Deletion mutant is synthetically lethal with MEN mutants complement(655137..656549) Saccharomyces cerevisiae 856158 NP_015371.1 CDS MCM16 NC_001148.3 656796 657341 D Protein involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; Mcm16p 656796..657341 Saccharomyces cerevisiae 856159 NP_015372.2 CDS MSF1 NC_001148.3 657526 658935 D Msf1p; Mitochondrial phenylalanyl-tRNA synthetase alpha subunit, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase 657526..658935 Saccharomyces cerevisiae 856160 NP_015373.1 CDS TAH18 NC_001148.3 659179 661050 D Tah18p; Protein with a potential role in DNA replication; displays synthetic lethal genetic interaction with the pol3-13 allele of POL3, which encodes DNA polymerase delta 659179..661050 Saccharomyces cerevisiae 856161 NP_015374.1 CDS ATG11 NC_001148.3 661134 664670 R Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy); Atg11p complement(661134..664670) Saccharomyces cerevisiae 856162 NP_015376.1 CDS MAK3 NC_001148.3 664957 665487 D Mak3p; Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus 664957..665487 Saccharomyces cerevisiae 856163 NP_015377.1 CDS NHP6A NC_001148.3 665690 665971 R Nhp6ap; High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Bp; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters complement(665690..665971) Saccharomyces cerevisiae 856165 NP_015379.1 CDS SMK1 NC_001148.3 666277 667443 D Smk1p; Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p 666277..667443 Saccharomyces cerevisiae 856167 NP_015380.1 CDS SEC8 NC_001148.3 667673 670870 D Sec8p; Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis 667673..670870 Saccharomyces cerevisiae 856168 NP_015381.1 CDS TFB4 NC_001148.3 671123 672139 D Tfb4p; Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p 671123..672139 Saccharomyces cerevisiae 856169 NP_015382.1 CDS BRR1 NC_001148.3 672468 673493 D snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed; Brr1p 672468..673493 Saccharomyces cerevisiae 856170 NP_015383.1 CDS YMC1 NC_001148.3 673748 674671 D Ymc1p; Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc2p 673748..674671 Saccharomyces cerevisiae 856171 NP_015385.1 CDS ARO7 NC_001148.3 674858 675628 R Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis; Aro7p complement(674858..675628) Saccharomyces cerevisiae 856173 NP_015386.1 CDS JID1 NC_001148.3 675974 676879 R Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae; Jid1p complement(675974..676879) Saccharomyces cerevisiae 856174 NP_015387.1 CDS FCY1 NC_001148.3 677162 677638 D Fcy1p; Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) 677162..677638 Saccharomyces cerevisiae 856175 NP_015388.1 CDS YPR063C NC_001148.3 677809 678317 R Ypr063cp; ER-localized protein of unknown function complement(join(677809..678190,678277..678317)) Saccharomyces cerevisiae 856176 NP_015390.1 CDS ROX1 NC_001148.3 679690 680796 D Rox1p; Heme-dependent repressor of hypoxic genes; contains an HMG domain that is responsible for DNA bending activity 679690..680796 Saccharomyces cerevisiae 856178 NP_015391.1 CDS UBA3 NC_001148.3 681210 682109 D Uba3p; Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern 681210..682109 Saccharomyces cerevisiae 856179 NP_015392.1 CDS ISA2 NC_001148.3 682217 682774 D Protein required for maturation of mitochondrial and cytosolic Fe/S proteins, localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations; Isa2p 682217..682774 Saccharomyces cerevisiae 856180 NP_015393.1 CDS HOS1 NC_001148.3 682938 684350 R Putative class I histone deacetylase (HDAC) with sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; deletion results in increased histone acetylation at rDNA repeats; interacts with the Tup1p-Ssn6p corepressor complex; Hos1p complement(682938..684350) Saccharomyces cerevisiae 856181 NP_015394.1 CDS SPE3 NC_001148.3 684553 685434 R Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells; Spe3p complement(684553..685434) Saccharomyces cerevisiae 856182 NP_015395.1 CDS MED1 NC_001148.3 685895 687595 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; Med1p 685895..687595 Saccharomyces cerevisiae 856183 NP_015396.1 CDS YPR071W NC_001148.3 688169 688804 D Ypr071wp; Putative membrane protein; YPR071W is not an essential gene 688169..688804 Saccharomyces cerevisiae 856184 NP_015397.1 CDS NOT5 NC_001148.3 690104 691786 D Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation; Not5p 690104..691786 Saccharomyces cerevisiae 856186 NP_015398.1 CDS LTP1 NC_001148.3 691930 692415 R Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine; Ltp1p complement(691930..692415) Saccharomyces cerevisiae 856187 NP_015399.1 CDS TKL1 NC_001148.3 692793 694835 R Transketolase, similar to Tkl2p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; Tkl1p complement(692793..694835) Saccharomyces cerevisiae 856188 NP_015400.1 CDS OPY2 NC_001148.3 695734 696816 R Opy2p; Integral membrane protein that functions in the signaling branch of the high-osmolarity glycerol (HOG) pathway; interacts with Ste50p; overproduction blocks cell cycle arrest in the presence of mating pheromone complement(695734..696816) Saccharomyces cerevisiae 856191 NP_015403.1 CDS YPR078C NC_001148.3 697144 698262 R Ypr078cp; Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible complement(697144..698262) Saccharomyces cerevisiae 856193 NP_015404.1 CDS MRL1 NC_001148.3 698867 700012 D Mrl1p; Membrane protein with similarity to mammalian mannose-6-phosphate receptors, possibly functions as a sorting receptor in the delivery of vacuolar hydrolases 698867..700012 Saccharomyces cerevisiae 856194 NP_015405.1 CDS TEF1 NC_001148.3 700592 701968 D Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; Tef1p 700592..701968 Saccharomyces cerevisiae 856195 NP_015406.1 CDS GRS2 NC_001148.3 702112 703968 R Protein with sequence similarity to Grs1p, which is a glycyl-tRNA synthetase; cannot substitute for Grs1p; possible pseudogene that is expressed at very low levels; Grs2p complement(702112..703968) Saccharomyces cerevisiae 856196 NP_015407.1 CDS DIB1 NC_001148.3 704223 704654 R Dib1p; 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein complement(704223..704654) Saccharomyces cerevisiae 856197 NP_015408.1 CDS MDM36 NC_001148.3 704852 706591 D Protein required for normal mitochondrial morphology and inheritance; Mdm36p 704852..706591 Saccharomyces cerevisiae 856198 NP_015409.1 CDS YPR084W NC_001148.3 706970 708340 D Ypr084wp; Putative protein of unknown function 706970..708340 Saccharomyces cerevisiae 856199 NP_015410.2 CDS YPR085C NC_001148.3 708495 709826 R Ypr085cp; Putative protein of unknown function; YPR085C is an essential gene complement(708495..709826) Saccharomyces cerevisiae 856200 NP_015411.1 CDS SUA7 NC_001148.3 710099 711136 D Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II; Sua7p 710099..711136 Saccharomyces cerevisiae 856201 NP_015413.1 CDS SRP54 NC_001148.3 711401 713026 R Srp54p; Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain complement(711401..713026) Saccharomyces cerevisiae 856203 NP_015414.2 CDS YPR089W NC_001148.3 713273 715939 D Ypr089wp; Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p 713273..715939 Saccharomyces cerevisiae 856204 NP_015416.1 CDS YPR091C NC_001148.3 716154 718466 R Ypr091cp; Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YPR091C is not an essential gene complement(716154..718466) Saccharomyces cerevisiae 856207 NP_015418.2 CDS ASR1 NC_001148.3 719556 720422 R Protein involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain; Asr1p complement(719556..720422) Saccharomyces cerevisiae 856208 NP_015419.1 CDS RDS3 NC_001148.3 720635 720958 D Zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance; Rds3p 720635..720958 Saccharomyces cerevisiae 856209 NP_015420.1 CDS SYT1 NC_001148.3 721035 724715 R Syt1p; Guanine nucleotide exchange factor (GEF) for Arf proteins; involved in vesicular transport; suppressor of ypt3 mutations; member of the Sec7-domain family complement(721035..724715) Saccharomyces cerevisiae 856210 NP_015421.1 CDS YPR096C NC_001148.3 724840 725142 R Ypr096cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments complement(724840..725142) Saccharomyces cerevisiae 856211 NP_015422.1 CDS YPR097W NC_001148.3 725392 728613 D Ypr097wp; Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies 725392..728613 Saccharomyces cerevisiae 856212 NP_015423.2 CDS YPR098C NC_001148.3 728945 729526 R Ypr098cp; Protein of unknown function, localized to the mitochondrial outer membrane complement(join(728945..729383,729480..729526)) Saccharomyces cerevisiae 856213 NP_015425.1 CDS MRPL51 NC_001148.3 729789 730211 D Mrpl51p; Mitochondrial ribosomal protein of the large subunit 729789..730211 Saccharomyces cerevisiae 856214 NP_015426.1 CDS SNT309 NC_001148.3 730490 731017 D Snt309p; Component of NineTeen complex (NTC) containing Prp19p involved in mRNA splicing, interacts physically and genetically with Prp19p 730490..731017 Saccharomyces cerevisiae 856216 NP_015427.1 CDS RPL11A NC_001148.3 731222 731746 R Rpl11ap; Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11 complement(731222..731746) Saccharomyces cerevisiae 856217 NP_015428.1 CDS PRE2 NC_001148.3 732347 733210 D Pre2p; 20S proteasome beta-type subunit, responsible for the chymotryptic activity of the proteasome 732347..733210 Saccharomyces cerevisiae 856218 NP_015429.1 CDS FHL1 NC_001148.3 733621 736431 R Transcriptional activator with similarity to DNA-binding domain of Drosophila forkhead but unable to bind DNA in vitro; required for rRNA processing; isolated as a suppressor of splicing factor prp4; Fhl1p complement(733621..736431) Saccharomyces cerevisiae 856219 NP_015430.1 CDS COG4 NC_001148.3 736982 739567 R Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; Cog4p complement(736982..739567) Saccharomyces cerevisiae 856220 NP_015431.1 CDS ISR1 NC_001148.3 740059 741390 D Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C; Isr1p 740059..741390 Saccharomyces cerevisiae 856221 NP_015432.1 CDS YTH1 NC_001148.3 741436 742062 R Yth1p; Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation complement(741436..742062) Saccharomyces cerevisiae 856222 NP_015433.1 CDS RPN7 NC_001148.3 742452 743741 D Rpn7p; Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits 742452..743741 Saccharomyces cerevisiae 856223 NP_878183.1 CDS YPR108W-A NC_001148.3 744173 744385 D Ypr108w-ap; Putative protein of unknown function; identified by fungal homology and RT-PCR 744173..744385 Saccharomyces cerevisiae 1466522 NP_015434.1 CDS YPR109W NC_001148.3 744687 745571 D Ypr109wp; Predicted membrane protein; dipoid deletion strain has high budding index 744687..745571 Saccharomyces cerevisiae 856225 NP_015435.1 CDS RPC40 NC_001148.3 745826 746833 R Rpc40p; RNA polymerase subunit, common to RNA polymerase I and III complement(745826..746833) Saccharomyces cerevisiae 856226 NP_015436.1 CDS DBF20 NC_001148.3 747304 748998 D Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; Dbf20p 747304..748998 Saccharomyces cerevisiae 856227 NP_015437.1 CDS MRD1 NC_001148.3 749254 751917 R Mrd1p; Essential conserved protein that associates with 35S precursor rRNA and is required for its initial processing at the A(0)-A(2) cleavage sites, shows partial nucleolar localization, contains five consensus RNA-binding domains complement(749254..751917) Saccharomyces cerevisiae 856228 NP_015438.1 CDS PIS1 NC_001148.3 752255 752917 D Pis1p; Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins 752255..752917 Saccharomyces cerevisiae 856229 NP_015439.1 CDS YPR114W NC_001148.3 753299 754246 D Ypr114wp; Putative protein of unknown function 753299..754246 Saccharomyces cerevisiae 856230 NP_015440.1 CDS YPR115W NC_001148.3 754875 758126 D Ypr115wp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm 754875..758126 Saccharomyces cerevisiae 856231 NP_015441.1 CDS YPR116W NC_001148.3 758646 759479 D Ypr116wp; Putative protein of unknown function 758646..759479 Saccharomyces cerevisiae 856232 NP_015442.1 CDS YPR117W NC_001148.3 760023 767492 D Ypr117wp; Putative protein of unknown function 760023..767492 Saccharomyces cerevisiae 856233 NP_015443.1 CDS MRI1 NC_001148.3 767750 768985 D Mri1p; Methylthioribose-1-phosphate isomerase, catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway 767750..768985 Saccharomyces cerevisiae 856234 NP_015444.1 CDS CLB2 NC_001148.3 771651 773126 D Clb2p; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome 771651..773126 Saccharomyces cerevisiae 856236 NP_015445.1 CDS CLB5 NC_001148.3 773873 775180 R Clb5p; B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase complement(773873..775180) Saccharomyces cerevisiae 856237 NP_015446.1 CDS THI22 NC_001148.3 778579 780297 D Thi22p; Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis 778579..780297 Saccharomyces cerevisiae 856239 NP_015447.1 CDS AXL1 NC_001148.3 782041 785667 D Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells; Axl1p 782041..785667 Saccharomyces cerevisiae 856240 NP_015449.1 CDS CTR1 NC_001148.3 786204 787424 D High-affinity copper transporter of the plasma membrane, mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; Ctr1p 786204..787424 Saccharomyces cerevisiae 856241 NP_015450.1 CDS YLH47 NC_001148.3 787957 789321 D Ylh47p; Mitochondrial inner membrane protein exposed to the mitochondrial matrix, associates with mitochondrial ribosomes, NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome 787957..789321 Saccharomyces cerevisiae 856243 NP_015452.1 CDS YPR127W NC_001148.3 790079 791116 D Ypr127wp; Putative protein of unknown function; expression is activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus 790079..791116 Saccharomyces cerevisiae 856245 NP_015453.1 CDS ANT1 NC_001148.3 791214 792200 R Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation; Ant1p complement(791214..792200) Saccharomyces cerevisiae 856246 NP_015454.1 CDS SCD6 NC_001148.3 792683 793732 D Scd6p; Protein containing an Lsm domain, may bind RNA and have a role in RNA processing; overproduction suppresses a null mutation in CHC1, which encodes the heavy chain of clathrin 792683..793732 Saccharomyces cerevisiae 856247 NP_015456.2 CDS NAT3 NC_001148.3 793907 794494 R Nat3p; Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met complement(793907..794494) Saccharomyces cerevisiae 856249 NP_015457.1 CDS RPS23B NC_001148.3 794961 795763 D Rps23bp; Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Ap and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal join(794961..795025,795391..795763) Saccharomyces cerevisiae 856250 NP_015458.1 CDS SPN1 NC_001148.3 795974 797206 R Spn1p; Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II, probable transcriptional elongation factor; metazoan homologs contain an acidic N terminus; mutations in the gene confer an Spt- phenotype complement(795974..797206) Saccharomyces cerevisiae 856251 NP_015459.1 CDS TOM5 NC_001148.3 797553 797705 D Tom5p; Small mitochondrial outer membrane protein crucial to a binding relay for the import of proteins into mitochondria; subunit on the outer mouth of the TOM channel that accepts precursors from the receptors Tom20p and Tom22p 797553..797705 Saccharomyces cerevisiae 856252 NP_015460.1 CDS MSS18 NC_001148.3 798047 798853 D Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions; Mss18p 798047..798853 Saccharomyces cerevisiae 856253 NP_015461.1 CDS CTF4 NC_001148.3 799230 802013 D Ctf4p; Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion 799230..802013 Saccharomyces cerevisiae 856254 NP_015463.1 CDS RRP9 NC_001148.3 802355 804076 D Rrp9p; Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein 802355..804076 Saccharomyces cerevisiae 856255 NP_058191.1 CDS YPR137C-B NC_001148.3 804997 810265 R Ypr137c-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(804997..808959,808961..810265)) Saccharomyces cerevisiae 856257 NP_058192.1 CDS YPR137C-A NC_001148.3 808943 810265 R Ypr137c-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(808943..810265) Saccharomyces cerevisiae 856258 NP_015464.1 CDS MEP3 NC_001148.3 810980 812449 R Mep3p; Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease complement(810980..812449) Saccharomyces cerevisiae 856260 NP_015465.1 CDS VPS66 NC_001148.3 813152 814054 R Vps66p; Cytoplasmic protein of unknown function involved in vacuolar protein sorting. complement(813152..814054) Saccharomyces cerevisiae 856261 NP_015466.1 CDS TAZ1 NC_001148.3 814387 815532 D Taz1p; Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; similar to human tafazzin, which is implicated in Barth syndrome 814387..815532 Saccharomyces cerevisiae 856262 NP_015467.1 CDS KAR3 NC_001148.3 815730 817919 R Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate; Kar3p complement(815730..817919) Saccharomyces cerevisiae 856263 NP_015469.1 CDS RRP15 NC_001148.3 818319 819071 D Rrp15p; Nucleolar protein, constituent of pre-60S ribosomal particles; required for processing of the 27S pre-rRNA at the A2 site to yield 5.8S and 25S rRNA 818319..819071 Saccharomyces cerevisiae 856264 NP_015470.1 CDS NOC4 NC_001148.3 819761 821419 R Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits; Noc4p complement(819761..821419) Saccharomyces cerevisiae 856267 NP_015471.1 CDS ASN1 NC_001148.3 822616 824334 D Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; Asn1p 822616..824334 Saccharomyces cerevisiae 856268 NP_001032572.1 CDS YPR145C-A NC_001148.3 824686 824922 R Ypr145c-ap; Putative protein of unknown function complement(824686..824922) Saccharomyces cerevisiae 3799978 NP_015473.1 CDS YPR147C NC_001148.3 825641 826555 R Ypr147cp; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS complement(825641..826555) Saccharomyces cerevisiae 856270 NP_015474.1 CDS YPR148C NC_001148.3 826829 828136 R Ypr148cp; Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern complement(826829..828136) Saccharomyces cerevisiae 856271 NP_015475.1 CDS NCE102 NC_001148.3 829914 830435 D Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); Nce102p 829914..830435 Saccharomyces cerevisiae 856272 NP_015477.1 CDS SUE1 NC_001148.3 831051 831671 R Sue1p; Mitochondrial protein required for degradation of unstable forms of cytochrome c complement(831051..831671) Saccharomyces cerevisiae 856274 NP_015478.1 CDS URN1 NC_001148.3 832057 833454 R Urn1p; Pre-mRNA splicing factor associated with the U2-U5-U6 snRNPs, the RES complex, and the Prp19-associated complex (NTC); null mutation displays synthetic genetic interactions with mutations affecting U2 snRNA and pre-mRNA splicing factors complement(832057..833454) Saccharomyces cerevisiae 856275 NP_015479.2 CDS YPR153W NC_001148.3 833685 834241 D Ypr153wp; Putative protein of unknown function join(833685..833689,833824..834241) Saccharomyces cerevisiae 856276 NP_015480.1 CDS PIN3 NC_001148.3 834561 835208 D Protein that induces appearance of [PIN+] prion when overproduced; Pin3p 834561..835208 Saccharomyces cerevisiae 856277 NP_015481.1 CDS NCA2 NC_001148.3 835559 837409 R Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p; Nca2p complement(835559..837409) Saccharomyces cerevisiae 856278 NP_015482.1 CDS TPO3 NC_001148.3 837905 839773 R Tpo3p; Polyamine transport protein specific for spermine; localizes to the plasma membrane; member of the major facilitator superfamily complement(837905..839773) Saccharomyces cerevisiae 856279 NP_015483.1 CDS YPR157W NC_001148.3 841262 842665 D Ypr157wp; Putative protein of unknown function 841262..842665 Saccharomyces cerevisiae 856280 NP_015484.1 CDS YPR158W NC_001148.3 843258 844016 D Ypr158wp; Putative protein of unknown function 843258..844016 Saccharomyces cerevisiae 856281 NP_058193.1 CDS YPR158W-B NC_001148.3 844705 849976 D Ypr158w-bp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes join(844705..846009,846011..849976) Saccharomyces cerevisiae 856283 NP_058194.1 CDS YPR158W-A NC_001148.3 844705 846027 D Ypr158w-ap; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag 844705..846027 Saccharomyces cerevisiae 856282 NP_058195.1 CDS YPR158C-D NC_001148.3 850985 856253 R Ypr158c-dp; Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes complement(join(850985..854947,854949..856253)) Saccharomyces cerevisiae 856284 NP_058196.1 CDS YPR158C-C NC_001148.3 854931 856253 R Ypr158c-cp; Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag complement(854931..856253) Saccharomyces cerevisiae 856285 NP_015485.1 CDS KRE6 NC_001148.3 857579 859741 D Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functionally redundant with Skn1p; Kre6p 857579..859741 Saccharomyces cerevisiae 856287 NP_878184.1 CDS YPR159C-A NC_001148.3 860310 860411 R Ypr159c-ap; Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching complement(860310..860411) Saccharomyces cerevisiae 1466523 NP_015486.1 CDS GPH1 NC_001148.3 861302 864010 D Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway; Gph1p 861302..864010 Saccharomyces cerevisiae 856289 NP_015487.1 CDS SGV1 NC_001148.3 864445 866418 R Sgv1p; Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p, which is the largest subunit of RNA polymerase II; regulated by Cak1p complement(864445..866418) Saccharomyces cerevisiae 856290 NP_015488.1 CDS ORC4 NC_001148.3 866711 868300 R Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; Orc4p complement(866711..868300) Saccharomyces cerevisiae 856291 NP_015489.1 CDS TIF3 NC_001148.3 868641 869951 R Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA; Tif3p complement(868641..869951) Saccharomyces cerevisiae 856292 NP_015490.1 CDS MMS1 NC_001148.3 870699 874922 D Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition; Mms1p 870699..874922 Saccharomyces cerevisiae 856293 NP_015491.1 CDS RHO1 NC_001148.3 875364 875993 D Rho1p; GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) 875364..875993 Saccharomyces cerevisiae 856294 NP_015492.1 CDS MRP2 NC_001148.3 876278 876625 R Mrp2p; Mitochondrial ribosomal protein of the small subunit complement(876278..876625) Saccharomyces cerevisiae 856295 NP_015493.1 CDS MET16 NC_001148.3 876843 877628 R Met16p; 3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism complement(876843..877628) Saccharomyces cerevisiae 856296 NP_015494.1 CDS NUT2 NC_001148.3 878072 878545 D Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; Nut2p 878072..878545 Saccharomyces cerevisiae 856297 NP_015495.2 CDS JIP5 NC_001148.3 878686 880164 D Nucleolar protein of unknown function, exhibits a physical interaction with Bre1p; Jip5p 878686..880164 Saccharomyces cerevisiae 856298 NP_001106949.1 CDS YPR170W-B NC_001148.3 883235 883591 D Ypr170w-bp; Putative protein of unknown function, conserved in fungi; partially overlaps the dubious genes YPR169W-A, YPR170W-A and YRP170C join(883235..883383,883483..883591) Saccharomyces cerevisiae 5848745 NP_015497.1 CDS BSP1 NC_001148.3 883824 885554 D Bsp1p; Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton 883824..885554 Saccharomyces cerevisiae 856301 NP_015498.1 CDS YPR172W NC_001148.3 885792 886394 D Ypr172wp; Protein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance 885792..886394 Saccharomyces cerevisiae 856302 NP_015499.1 CDS VPS4 NC_001148.3 886520 887833 R Vps4p; AAA-type ATPase that is regulated by Vta1p; required for late endosome to vacuole transport; catalyzes the release of an endosomal membrane-associated class E VPS protein complex; regulates cellular sterol metabolism complement(886520..887833) Saccharomyces cerevisiae 856303 NP_015500.1 CDS YPR174C NC_001148.3 888039 888704 R Ypr174cp; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate complement(888039..888704) Saccharomyces cerevisiae 856304 NP_015501.2 CDS DPB2 NC_001148.3 888970 891039 D Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate; Dpb2p 888970..891039 Saccharomyces cerevisiae 856305 NP_015502.1 CDS BET2 NC_001148.3 891097 892074 R Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p; Bet2p complement(891097..892074) Saccharomyces cerevisiae 856306 NP_015504.1 CDS PRP4 NC_001148.3 892328 893725 D Splicing factor, component of the U4/U6-U5 snRNP complex; Prp4p 892328..893725 Saccharomyces cerevisiae 856307 NP_015505.1 CDS HDA3 NC_001148.3 893793 895760 R Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p; Hda3p complement(893793..895760) Saccharomyces cerevisiae 856309 NP_015506.1 CDS AOS1 NC_001148.3 895957 897000 D Nuclear protein that acts as a heterodimer with Uba2p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; Aos1p 895957..897000 Saccharomyces cerevisiae 856310 NP_015507.1 CDS SEC23 NC_001148.3 897357 899663 R Sec23p; GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p complement(897357..899663) Saccharomyces cerevisiae 856311 NP_015508.1 CDS SMX3 NC_001148.3 900190 900450 D Smx3p; Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F 900190..900450 Saccharomyces cerevisiae 856312 NP_015509.1 CDS DPM1 NC_001148.3 900751 901554 D Dpm1p; Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation 900751..901554 Saccharomyces cerevisiae 856313 NP_015510.1 CDS GDB1 NC_001148.3 902040 906650 D Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation; phosphorylated in mitochondria; Gdb1p 902040..906650 Saccharomyces cerevisiae 856314 NP_015511.1 CDS ATG13 NC_001148.3 907214 909430 D Phosphorylated protein that interacts with Vac8p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway and autophagy; Atg13p 907214..909430 Saccharomyces cerevisiae 856315 NP_015512.1 CDS PZF1 NC_001148.3 909729 911018 R Transcription factor IIIA (TFIIIA), essential protein with nine C2H2 Zn-fingers, binds the 5S rRNA gene through the zinc finger domain and directs assembly of a multiprotein initiation complex for RNA polymerase III; also binds DNA; Pzf1p complement(909729..911018) Saccharomyces cerevisiae 856316 NP_015513.1 CDS RPO26 NC_001148.3 911253 911796 D Rpo26p; RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit join(911253..911272,911349..911796) Saccharomyces cerevisiae 856317 NP_015514.1 CDS MLC2 NC_001148.3 911984 912475 R Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring; Mlc2p complement(911984..912475) Saccharomyces cerevisiae 856318 NP_015515.1 CDS SKI3 NC_001148.3 912660 916958 D Ski3p; Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski2p and Ski8p; required for repressing propagation of dsRNA viruses 912660..916958 Saccharomyces cerevisiae 856319 NP_015516.1 CDS RPC82 NC_001148.3 917073 919037 R Rpc82p; RNA polymerase III subunit C82 complement(917073..919037) Saccharomyces cerevisiae 856320 NP_015517.1 CDS QCR2 NC_001148.3 919377 920483 D Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme; Qcr2p 919377..920483 Saccharomyces cerevisiae 856321 NP_015518.1 CDS AQY1 NC_001148.3 921856 922773 D Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance; Aqy1p 921856..922773 Saccharomyces cerevisiae 856322 NP_015519.1 CDS HPA2 NC_001148.3 922905 923375 R Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; Hpa2p complement(922905..923375) Saccharomyces cerevisiae 856323 NP_015520.1 CDS OPT2 NC_001148.3 924300 926933 R Opt2p; Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans complement(924300..926933) Saccharomyces cerevisiae 856324 NP_015522.1 CDS YPR196W NC_001148.3 931372 932784 D Ypr196wp; Putative maltose activator 931372..932784 Saccharomyces cerevisiae 856326 NP_015524.1 CDS SGE1 NC_001148.3 934030 935661 D Sge1p; Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations 934030..935661 Saccharomyces cerevisiae 856327 NP_015525.1 CDS ARR1 NC_001148.3 938144 939028 R Transcriptional activator of the bZIP family, required for transcription of genes involved in resistance to arsenic compounds; Arr1p complement(938144..939028) Saccharomyces cerevisiae 856329 NP_015526.1 CDS ARR2 NC_001148.3 939275 939667 R Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p; Arr2p complement(939275..939667) Saccharomyces cerevisiae 856330 NP_015527.1 CDS ARR3 NC_001148.3 939918 941132 D Arsenite transporter of the plasma membrane, required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite; Arr3p 939918..941132 Saccharomyces cerevisiae 856331 NP_015528.1 CDS YPR202W NC_001148.3 943028 943892 D Ypr202wp; Putative protein of unknown function with similarity to telomere-encoded helicases; YPR202W is not an essential gene; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo join(943028..943046,943195..943892) Saccharomyces cerevisiae 856332 NP_015529.1 CDS YPR203W NC_001148.3 943876 944184 D Ypr203wp; Putative protein of unknown function 943876..944184 Saccharomyces cerevisiae 856333 NP_015530.1 CDS YPR204W NC_001148.3 944599 947697 D Ypr204wp; Helicase-like protein encoded within the telomeric Y' element 944599..947697 Saccharomyces cerevisiae 856334 TP(UGG)A tRNA TRN1 NC_001133.7 139154 139256 D tRNA-Pro join(139154..139189,139221..139256) Saccharomyces cerevisiae 851227 TA(UGC)A tRNA TA(UGC)A NC_001133.7 166268 166340 D tRNA-Ala 166268..166340 Saccharomyces cerevisiae 851270 TL(CAA)A tRNA SUP56 NC_001133.7 181135 181248 D tRNA-Leu join(181135..181172,181205..181248) Saccharomyces cerevisiae 851277 TS(AGA)A tRNA TS(AGA)A NC_001133.7 182516 182597 R tRNA-Ser complement(182516..182597) Saccharomyces cerevisiae 851278 TL(UAA)B1 tRNA TL(UAA)B1 NC_001134.7 9583 9666 D tRNA-Leu 9583..9666 Saccharomyces cerevisiae 852166 TF(GAA)B tRNA TF(GAA)B NC_001134.7 36395 36485 D tRNA-Phe join(36395..36431,36450..36485) Saccharomyces cerevisiae 852176 TI(AAU)B tRNA TI(AAU)B NC_001134.7 197497 197570 D tRNA-Ile 197497..197570 Saccharomyces cerevisiae 852267 TG(GCC)B tRNA TG(GCC)B NC_001134.7 197632 197702 R tRNA-Gly complement(197632..197702) Saccharomyces cerevisiae 852268 TS(AGA)B tRNA TS(AGA)B NC_001134.7 227078 227159 D tRNA-Ser 227078..227159 Saccharomyces cerevisiae 852281 TT(AGU)B tRNA TT(AGU)B NC_001134.7 266378 266450 D tRNA-Thr 266378..266450 Saccharomyces cerevisiae 852301 TV(UAC)B tRNA TV(UAC)B NC_001134.7 326791 326864 R tRNA-Val complement(326791..326864) Saccharomyces cerevisiae 852333 TL(UAA)B2 tRNA TL(UAA)B2 NC_001134.7 347601 347684 D tRNA-Leu 347601..347684 Saccharomyces cerevisiae 852345 TQ(UUG)B tRNA TQ(UUG)B NC_001134.7 350825 350896 D tRNA-Gln 350825..350896 Saccharomyces cerevisiae 852347 TR(UCU)B tRNA TR(UCU)B NC_001134.7 405872 405943 D tRNA-Arg 405872..405943 Saccharomyces cerevisiae 852374 TD(GUC)B tRNA TD(GUC)B NC_001134.7 405954 406025 D tRNA-Asp 405954..406025 Saccharomyces cerevisiae 852375 TC(GCA)B tRNA TC(GCA)B NC_001134.7 643002 643073 R tRNA-Cys complement(643002..643073) Saccharomyces cerevisiae 852509 TE(UUC)B tRNA TE(UUC)B NC_001134.7 645162 645233 D tRNA-Glu 645162..645233 Saccharomyces cerevisiae 852510 TE(UUC)C tRNA TE(UUC)C NC_001135.4 82462 82533 R tRNA-Glu complement(82462..82533) Saccharomyces cerevisiae 850337 TL(CAA)C tRNA SUP53 NC_001135.4 90859 90972 D tRNA-Leu join(90859..90896,90929..90972) Saccharomyces cerevisiae 850341 TP(AGG)C tRNA SUF2 NC_001135.4 123575 123646 R tRNA-Pro complement(123575..123646) Saccharomyces cerevisiae 850363 TN(GUU)C tRNA TN(GUU)C NC_001135.4 127714 127787 D tRNA-Asn 127714..127787 Saccharomyces cerevisiae 850365 TG(GCC)C tRNA SUF16 NC_001135.4 142699 142769 R tRNA-Gly complement(142699..142769) Saccharomyces cerevisiae 850374 TM(CAU)C tRNA TM(CAU)C NC_001135.4 149918 149989 D tRNA-Met 149918..149989 Saccharomyces cerevisiae 850378 TK(CUU)C tRNA TK(CUU)C NC_001135.4 151282 151354 R tRNA-Lys complement(151282..151354) Saccharomyces cerevisiae 850379 TQ(UUG)C tRNA TQ(UUG)C NC_001135.4 168297 168368 R tRNA-Gln complement(168297..168368) Saccharomyces cerevisiae 850393 TS(CGA)C tRNA SUP61 NC_001135.4 227938 228038 D tRNA-Ser join(227938..227974,227994..228038) Saccharomyces cerevisiae 850426 TT(AGU)C tRNA TT(AGU)C NC_001135.4 295481 295553 D tRNA-Thr 295481..295553 Saccharomyces cerevisiae 850461 TG(GCC)D1 tRNA TG(GCC)D1 NC_001136.8 83549 83619 D tRNA-Gly 83549..83619 Saccharomyces cerevisiae 851316 TK(UUU)D tRNA TK(UUU)D NC_001136.8 359577 359672 D tRNA-Lys join(359577..359613,359637..359672) Saccharomyces cerevisiae 851505 TA(AGC)D tRNA TA(AGC)D NC_001136.8 410376 410448 D tRNA-Ala 410376..410448 Saccharomyces cerevisiae 851538 TT(AGU)D tRNA TT(AGU)D NC_001136.8 434261 434333 R tRNA-Thr complement(434261..434333) Saccharomyces cerevisiae 851555 TS(AGA)D1 tRNA TS(AGA)D1 NC_001136.8 437769 437850 D tRNA-Ser 437769..437850 Saccharomyces cerevisiae 851556 TV(UAC)D tRNA TV(UAC)D NC_001136.8 488794 488867 R tRNA-Val complement(488794..488867) Saccharomyces cerevisiae 851586 TL(UAA)D tRNA TL(UAA)D NC_001136.8 519740 519823 D tRNA-Leu 519740..519823 Saccharomyces cerevisiae 851601 TQ(UUG)D1 tRNA TQ(UUG)D1 NC_001136.8 520969 521040 D tRNA-Gln 520969..521040 Saccharomyces cerevisiae 851603 TR(UCU)D tRNA TR(UCU)D NC_001136.8 568880 568951 D tRNA-Arg 568880..568951 Saccharomyces cerevisiae 851629 TD(GUC)D tRNA TD(GUC)D NC_001136.8 568962 569033 D tRNA-Asp 568962..569033 Saccharomyces cerevisiae 851630 TR(ACG)D tRNA TR(ACG)D NC_001136.8 619966 620038 R tRNA-Arg complement(619966..620038) Saccharomyces cerevisiae 851662 TQ(UUG)D2 tRNA TQ(UUG)D2 NC_001136.8 645150 645221 R tRNA-Gln complement(645150..645221) Saccharomyces cerevisiae 851673 TI(AAU)D tRNA TI(AAU)D NC_001136.8 668004 668077 R tRNA-Ile complement(668004..668077) Saccharomyces cerevisiae 851684 TQ(UUG)D3 tRNA TQ(UUG)D3 NC_001136.8 802728 802799 R tRNA-Gln complement(802728..802799) Saccharomyces cerevisiae 851749 TI(UAU)D tRNA TI(UAU)D NC_001136.8 884358 884490 D tRNA-Ile join(884358..884394,884455..884490) Saccharomyces cerevisiae 851796 TY(GUA)D tRNA SUP2 NC_001136.8 946308 946396 D tRNA-Tyr join(946308..946346,946361..946396) Saccharomyces cerevisiae 851828 TS(AGA)D2 tRNA TS(AGA)D2 NC_001136.8 980970 981051 R tRNA-Ser complement(980970..981051) Saccharomyces cerevisiae 851849 TG(GCC)D2 tRNA TG(GCC)D2 NC_001136.8 992828 992898 D tRNA-Gly 992828..992898 Saccharomyces cerevisiae 851854 TE(CUC)D tRNA TE(CUC)D NC_001136.8 1017203 1017274 R tRNA-Glu complement(1017203..1017274) Saccharomyces cerevisiae 851871 TV(CAC)D tRNA TV(CAC)D NC_001136.8 1075468 1075540 R tRNA-Val complement(1075468..1075540) Saccharomyces cerevisiae 851901 TF(GAA)D tRNA TF(GAA)D NC_001136.8 1095367 1095458 R tRNA-Phe complement(join(1095367..1095402,1095422..1095458)) Saccharomyces cerevisiae 851912 TX(XXX)D tRNA TX(XXX)D NC_001136.8 1150836 1150935 R tRNA-OTHER complement(1150836..1150935) Saccharomyces cerevisiae 851941 TM(CAU)D tRNA EMT1 NC_001136.8 1175823 1175895 D tRNA-Met 1175823..1175895 Saccharomyces cerevisiae 851951 TK(CUU)D1 tRNA TK(CUU)D1 NC_001136.8 1201742 1201814 D tRNA-Lys 1201742..1201814 Saccharomyces cerevisiae 851966 TG(CCC)D tRNA SUF3 NC_001136.8 1257000 1257071 R tRNA-Gly complement(1257000..1257071) Saccharomyces cerevisiae 851999 TS(AGA)D3 tRNA TS(AGA)D3 NC_001136.8 1305622 1305704 D tRNA-Ser 1305622..1305704 Saccharomyces cerevisiae 852029 TK(CUU)D2 tRNA TK(CUU)D2 NC_001136.8 1352458 1352530 R tRNA-Lys complement(1352458..1352530) Saccharomyces cerevisiae 852055 TL(CAA)D tRNA TL(CAA)D NC_001136.8 1461707 1461821 R tRNA-Leu complement(join(1461707..1461750,1461784..1461821)) Saccharomyces cerevisiae 852118 TT(AGU)I1 tRNA TT(AGU)I1 NC_001141.1 175028 175100 D tRNA-Thr 175028..175100 Saccharomyces cerevisiae 854705 TI(AAU)I1 tRNA TI(AAU)I1 NC_001141.1 183437 183510 R tRNA-Ile complement(183437..183510) Saccharomyces cerevisiae 854712 TE(CUC)I tRNA TE(CUC)I NC_001141.1 197589 197660 D tRNA-Glu 197589..197660 Saccharomyces cerevisiae 854720 TI(AAU)I2 tRNA TI(AAU)I2 NC_001141.1 210662 210735 D tRNA-Ile 210662..210735 Saccharomyces cerevisiae 854730 TS(UGA)I tRNA SUP17 NC_001141.1 248847 248928 D tRNA-Ser 248847..248928 Saccharomyces cerevisiae 854754 TK(CUU)I tRNA TK(CUU)I NC_001141.1 300227 300298 R tRNA-Lys complement(300227..300298) Saccharomyces cerevisiae 854782 TD(GUC)I1 tRNA TD(GUC)I1 NC_001141.1 324301 324372 R tRNA-Asp complement(324301..324372) Saccharomyces cerevisiae 854798 TT(AGU)I2 tRNA TT(AGU)I2 NC_001141.1 325746 325818 D tRNA-Thr 325746..325818 Saccharomyces cerevisiae 854800 TD(GUC)I2 tRNA TD(GUC)I2 NC_001141.1 336346 336417 R tRNA-Asp complement(336346..336417) Saccharomyces cerevisiae 854807 TE(UUC)I tRNA TE(UUC)I NC_001141.1 370414 370485 D tRNA-Glu 370414..370485 Saccharomyces cerevisiae 854823 TP(UGG)Q tRNA TP(UGG)Q NC_001224.1 731 802 D tRNA-Pro 731..802 Saccharomyces cerevisiae 854578 TW(UCA)Q tRNA TW(UCA)Q NC_001224.1 9374 9447 D tRNA-Trp 9374..9447 Saccharomyces cerevisiae 854581 TE(UUC)Q tRNA TE(UUC)Q NC_001224.1 35373 35444 D tRNA-Glu 35373..35444 Saccharomyces cerevisiae 854603 TS(UGA)Q2 tRNA TS(UGA)Q2 NC_001224.1 48201 48290 D tRNA-Ser 48201..48290 Saccharomyces cerevisiae 854585 TT(UGU)Q1 tRNA TT(UGU)Q1 NC_001224.1 63862 63937 D tRNA-Thr 63862..63937 Saccharomyces cerevisiae 854591 TC(GCA)Q tRNA TC(GCA)Q NC_001224.1 64415 64490 D tRNA-Cys 64415..64490 Saccharomyces cerevisiae 854606 TH(GUG)Q tRNA TH(GUG)Q NC_001224.1 64596 64670 D tRNA-His 64596..64670 Saccharomyces cerevisiae 854607 TL(UAA)Q tRNA TL(UAA)Q NC_001224.1 66095 66179 D tRNA-Leu 66095..66179 Saccharomyces cerevisiae 854609 TQ(UUG)Q tRNA TQ(UUG)Q NC_001224.1 66210 66285 D tRNA-Gln 66210..66285 Saccharomyces cerevisiae 854610 TK(UUU)Q tRNA TK(UUU)Q NC_001224.1 67061 67134 D tRNA-Lys 67061..67134 Saccharomyces cerevisiae 854611 TR(UCU)Q1 tRNA TR(UCU)Q1 NC_001224.1 67309 67381 D tRNA-Arg 67309..67381 Saccharomyces cerevisiae 854612 TG(UCC)Q tRNA TG(UCC)Q NC_001224.1 67468 67542 D tRNA-Gly 67468..67542 Saccharomyces cerevisiae 854613 TD(GUC)Q tRNA TD(GUC)Q NC_001224.1 68322 68396 D tRNA-Asp 68322..68396 Saccharomyces cerevisiae 854614 TS(GCU)Q1 tRNA TS(GCU)Q1 NC_001224.1 69203 69288 D tRNA-Ser 69203..69288 Saccharomyces cerevisiae 854615 TR(ACG)Q2 tRNA TR(ACG)Q2 NC_001224.1 69289 69362 D tRNA-Arg 69289..69362 Saccharomyces cerevisiae 854616 TA(UGC)Q tRNA TA(UGC)Q NC_001224.1 69846 69921 D tRNA-Ala 69846..69921 Saccharomyces cerevisiae 854617 TI(GAU)Q tRNA TI(GAU)Q NC_001224.1 70162 70237 D tRNA-Ile 70162..70237 Saccharomyces cerevisiae 854618 TY(GUA)Q tRNA TY(GUA)Q NC_001224.1 70824 70907 D tRNA-Tyr 70824..70907 Saccharomyces cerevisiae 854619 TN(GUU)Q tRNA TN(GUU)Q NC_001224.1 71433 71503 D tRNA-Asn 71433..71503 Saccharomyces cerevisiae 854620 TM(CAU)Q1 tRNA TM(CAU)Q1 NC_001224.1 72630 72705 D tRNA-Met 72630..72705 Saccharomyces cerevisiae 854621 TF(GAA)Q tRNA TF(GAA)Q NC_001224.1 77431 77505 D tRNA-Phe 77431..77505 Saccharomyces cerevisiae 854624 TT(XXX)Q2 tRNA TT(XXX)Q2 NC_001224.1 78089 78162 R tRNA-Thr complement(78089..78162) Saccharomyces cerevisiae 854625 TV(UAC)Q tRNA TV(UAC)Q NC_001224.1 78533 78608 D tRNA-Val 78533..78608 Saccharomyces cerevisiae 854626 TM(CAU)Q2 tRNA TM(CAU)Q2 NC_001224.1 85035 85112 D tRNA-Met 85035..85112 Saccharomyces cerevisiae 854628 TG(GCC)E tRNA TG(GCC)E NC_001137.2 61890 61960 R tRNA-Gly complement(61890..61960) Saccharomyces cerevisiae 856661 TS(AGA)E tRNA TS(AGA)E NC_001137.2 86604 86685 D tRNA-Ser 86604..86685 Saccharomyces cerevisiae 856679 TM(CAU)E tRNA IMT4 NC_001137.2 100133 100204 D tRNA-Met 100133..100204 Saccharomyces cerevisiae 856685 TQ(UUG)E2 tRNA TQ(UUG)E2 NC_001137.2 131082 131153 R tRNA-Gln complement(131082..131153) Saccharomyces cerevisiae 856703 TK(CUU)E1 tRNA TK(CUU)E1 NC_001137.2 135425 135497 R tRNA-Lys complement(135425..135497) Saccharomyces cerevisiae 856706 TR(UCU)E tRNA TR(UCU)E NC_001137.2 138666 138737 D tRNA-Arg 138666..138737 Saccharomyces cerevisiae 856708 TE(UUC)E1 tRNA TE(UUC)E1 NC_001137.2 177098 177169 D tRNA-Glu 177098..177169 Saccharomyces cerevisiae 856730 TH(GUG)E1 tRNA TH(GUG)E1 NC_001137.2 207356 207427 D tRNA-His 207356..207427 Saccharomyces cerevisiae 856747 TQ(UUG)E1 tRNA TQ(UUG)E1 NC_001137.2 250285 250356 D tRNA-Gln 250285..250356 Saccharomyces cerevisiae 856773 TS(UGA)E tRNA SUP19 NC_001137.2 288441 288522 R tRNA-Ser complement(288441..288522) Saccharomyces cerevisiae 856795 TA(UGC)E tRNA TA(UGC)E NC_001137.2 312019 312091 R tRNA-Ala complement(312019..312091) Saccharomyces cerevisiae 856808 TE(UUC)E2 tRNA TE(UUC)E2 NC_001137.2 354930 355001 D tRNA-Glu 354930..355001 Saccharomyces cerevisiae 856833 TH(GUG)E2 tRNA TH(GUG)E2 NC_001137.2 434537 434608 R tRNA-His complement(434537..434608) Saccharomyces cerevisiae 856871 TK(CUU)E2 tRNA TK(CUU)E2 NC_001137.2 435748 435820 R tRNA-Lys complement(435748..435820) Saccharomyces cerevisiae 856872 TV(AAC)E1 tRNA TV(AAC)E1 NC_001137.2 438696 438769 D tRNA-Val 438696..438769 Saccharomyces cerevisiae 856875 TI(AAU)E1 tRNA TI(AAU)E1 NC_001137.2 443198 443271 R tRNA-Ile complement(443198..443271) Saccharomyces cerevisiae 856878 TV(AAC)E2 tRNA TV(AAC)E2 NC_001137.2 469452 469525 D tRNA-Val 469452..469525 Saccharomyces cerevisiae 856894 TE(UUC)E3 tRNA TE(UUC)E3 NC_001137.2 487326 487397 R tRNA-Glu complement(487326..487397) Saccharomyces cerevisiae 856902 TR(ACG)E tRNA TR(ACG)E NC_001137.2 492347 492419 R tRNA-Arg complement(492347..492419) Saccharomyces cerevisiae 856905 TI(AAU)E2 tRNA TI(AAU)E2 NC_001137.2 551280 551353 R tRNA-Ile complement(551280..551353) Saccharomyces cerevisiae 856929 TP(UGG)F tRNA SUF9 NC_001138.4 101370 101472 D tRNA-Pro join(101370..101405,101437..101472) Saccharomyces cerevisiae 850526 TN(GUU)F tRNA TN(GUU)F NC_001138.4 137481 137554 R tRNA-Asn complement(137481..137554) Saccharomyces cerevisiae 850546 TF(GAA)F tRNA TF(GAA)F NC_001138.4 157910 158001 R tRNA-Phe complement(join(157910..157945,157965..158001)) Saccharomyces cerevisiae 850557 TG(GCC)F1 tRNA SUF20 NC_001138.4 162222 162292 D tRNA-Gly 162222..162292 Saccharomyces cerevisiae 850560 TY(GUA)F1 tRNA SUP11 NC_001138.4 167430 167518 D tRNA-Tyr join(167430..167468,167483..167518) Saccharomyces cerevisiae 850564 TG(GCC)F2 tRNA TG(GCC)F2 NC_001138.4 180966 181036 R tRNA-Gly complement(180966..181036) Saccharomyces cerevisiae 850571 TS(GCU)F tRNA TS(GCU)F NC_001138.4 191501 191601 R tRNA-Ser complement(join(191501..191545,191565..191601)) Saccharomyces cerevisiae 850575 TA(AGC)F tRNA TA(AGC)F NC_001138.4 204912 204984 R tRNA-Ala complement(204912..204984) Saccharomyces cerevisiae 850582 TY(GUA)F2 tRNA SUP6 NC_001138.4 210607 210695 R tRNA-Tyr complement(join(210607..210642,210657..210695)) Saccharomyces cerevisiae 850586 TK(CUU)F tRNA TK(CUU)F NC_001138.4 226676 226747 R tRNA-Lys complement(226676..226747) Saccharomyces cerevisiae 850596 TV(AAC)G3 tRNA TV(AAC)G3 NC_001139.8 73828 73901 R tRNA-Val complement(73828..73901) Saccharomyces cerevisiae 852627 TH(GUG)G1 tRNA TH(GUG)G1 NC_001139.8 110629 110700 R tRNA-His complement(110629..110700) Saccharomyces cerevisiae 852668 TK(UUU)G1 tRNA TK(UUU)G1 NC_001139.8 115492 115587 D tRNA-Lys join(115492..115528,115552..115587) Saccharomyces cerevisiae 852671 TK(CUU)G1 tRNA TK(CUU)G1 NC_001139.8 122273 122345 D tRNA-Lys 122273..122345 Saccharomyces cerevisiae 852674 TK(CUU)G2 tRNA TK(CUU)G2 NC_001139.8 185720 185792 R tRNA-Lys complement(185720..185792) Saccharomyces cerevisiae 852706 TL(CAA)G1 tRNA TL(CAA)G1 NC_001139.8 205527 205640 D tRNA-Leu join(205527..205564,205597..205640) Saccharomyces cerevisiae 852718 TW(CCA)G1 tRNA TW(CCA)G1 NC_001139.8 287356 287461 R tRNA-Trp complement(join(287356..287391,287426..287461)) Saccharomyces cerevisiae 852759 TH(GUG)G2 tRNA TH(GUG)G2 NC_001139.8 319784 319855 D tRNA-His 319784..319855 Saccharomyces cerevisiae 852781 TE(UUC)G1 tRNA SOE1 NC_001139.8 328586 328657 D tRNA-Glu 328586..328657 Saccharomyces cerevisiae 852784 TE(UUC)G2 tRNA TE(UUC)G2 NC_001139.8 401530 401601 R tRNA-Glu complement(401530..401601) Saccharomyces cerevisiae 852827 TR(UCU)G1 tRNA TR(UCU)G1 NC_001139.8 405473 405544 D tRNA-Arg 405473..405544 Saccharomyces cerevisiae 852831 TV(AAC)G1 tRNA TV(AAC)G1 NC_001139.8 412297 412370 R tRNA-Val complement(412297..412370) Saccharomyces cerevisiae 852836 TL(CAA)G2 tRNA SUP54 NC_001139.8 423098 423211 D tRNA-Leu join(423098..423135,423168..423211) Saccharomyces cerevisiae 852843 TF(GAA)G tRNA TF(GAA)G NC_001139.8 440721 440812 R tRNA-Phe complement(join(440721..440756,440776..440812)) Saccharomyces cerevisiae 852855 TD(GUC)G1 tRNA TD(GUC)G1 NC_001139.8 531614 531685 D tRNA-Asp 531614..531685 Saccharomyces cerevisiae 852907 TE(UUC)G3 tRNA TE(UUC)G3 NC_001139.8 541854 541925 D tRNA-Glu 541854..541925 Saccharomyces cerevisiae 852914 TD(GUC)G2 tRNA TD(GUC)G2 NC_001139.8 544581 544652 R tRNA-Asp complement(544581..544652) Saccharomyces cerevisiae 852917 TS(AGA)G tRNA TS(AGA)G NC_001139.8 561666 561747 R tRNA-Ser complement(561666..561747) Saccharomyces cerevisiae 852927 TT(UGU)G1 tRNA TT(UGU)G1 NC_001139.8 661753 661824 D tRNA-Thr 661753..661824 Saccharomyces cerevisiae 852981 TL(GAG)G tRNA TL(GAG)G NC_001139.8 700679 700760 D tRNA-Leu 700679..700760 Saccharomyces cerevisiae 852999 TK(UUU)G2 tRNA TK(UUU)G2 NC_001139.8 700957 701052 R tRNA-Lys complement(join(700957..700992,701016..701052)) Saccharomyces cerevisiae 853000 TC(GCA)G tRNA TC(GCA)G NC_001139.8 707112 707183 R tRNA-Cys complement(707112..707183) Saccharomyces cerevisiae 853004 TN(GUU)G tRNA TN(GUU)G NC_001139.8 731142 731215 D tRNA-Asn 731142..731215 Saccharomyces cerevisiae 853018 TR(UCU)G3 tRNA TR(UCU)G3 NC_001139.8 736345 736416 D tRNA-Arg 736345..736416 Saccharomyces cerevisiae 853022 TI(AAU)G tRNA TI(AAU)G NC_001139.8 739127 739200 D tRNA-Ile 739127..739200 Saccharomyces cerevisiae 853024 TA(AGC)G tRNA TA(AGC)G NC_001139.8 774354 774426 D tRNA-Ala 774354..774426 Saccharomyces cerevisiae 853044 TG(UCC)G tRNA SUF4 NC_001139.8 779621 779692 D tRNA-Gly 779621..779692 Saccharomyces cerevisiae 853046 TA(UGC)G tRNA TA(UGC)G NC_001139.8 794422 794494 D tRNA-Ala 794422..794494 Saccharomyces cerevisiae 853054 TV(AAC)G2 tRNA TV(AAC)G2 NC_001139.8 823487 823560 D tRNA-Val 823487..823560 Saccharomyces cerevisiae 853070 TR(UCU)G2 tRNA TR(UCU)G2 NC_001139.8 828728 828799 R tRNA-Arg complement(828728..828799) Saccharomyces cerevisiae 853074 TG(GCC)G1 tRNA TG(GCC)G1 NC_001139.8 845654 845724 D tRNA-Gly 845654..845724 Saccharomyces cerevisiae 853084 TL(CAA)G3 tRNA TL(CAA)G3 NC_001139.8 857383 857496 R tRNA-Leu complement(join(857383..857426,857459..857496)) Saccharomyces cerevisiae 853092 TK(CUU)G3 tRNA TK(CUU)G3 NC_001139.8 876399 876471 D tRNA-Lys 876399..876471 Saccharomyces cerevisiae 853101 TW(CCA)G2 tRNA TW(CCA)G2 NC_001139.8 878715 878820 R tRNA-Trp complement(join(878715..878750,878785..878820)) Saccharomyces cerevisiae 853103 TG(GCC)G2 tRNA TG(GCC)G2 NC_001139.8 930956 931026 R tRNA-Gly complement(930956..931026) Saccharomyces cerevisiae 853132 TT(UGU)G2 tRNA TT(UGU)G2 NC_001139.8 1004222 1004293 D tRNA-Thr 1004222..1004293 Saccharomyces cerevisiae 853171 TH(GUG)H tRNA TH(GUG)H NC_001140.5 62753 62824 D tRNA-His 62753..62824 Saccharomyces cerevisiae 856363 TV(AAC)H tRNA TV(AAC)H NC_001140.5 85294 85367 R tRNA-Val complement(85294..85367) Saccharomyces cerevisiae 856378 TT(AGU)H tRNA TT(AGU)H NC_001140.5 116102 116174 R tRNA-Thr complement(116102..116174) Saccharomyces cerevisiae 856396 TS(AGA)H tRNA TS(AGA)H NC_001140.5 133019 133100 R tRNA-Ser complement(133019..133100) Saccharomyces cerevisiae 856406 TQ(UUG)H tRNA TQ(UUG)H NC_001140.5 134314 134385 D tRNA-Gln 134314..134385 Saccharomyces cerevisiae 856407 TA(AGC)H tRNA TA(AGC)H NC_001140.5 146235 146307 R tRNA-Ala complement(146235..146307) Saccharomyces cerevisiae 856414 TF(GAA)H1 tRNA TF(GAA)H1 NC_001140.5 237850 237941 R tRNA-Phe complement(join(237850..237885,237905..237941)) Saccharomyces cerevisiae 856469 TF(GAA)H2 tRNA TF(GAA)H2 NC_001140.5 358481 358572 R tRNA-Phe complement(join(358481..358516,358536..358572)) Saccharomyces cerevisiae 856525 TP(UGG)H tRNA SUF8 NC_001140.5 388896 388998 R tRNA-Pro complement(join(388896..388931,388963..388998)) Saccharomyces cerevisiae 856549 TT(UGU)H tRNA TT(UGU)H NC_001140.5 466990 467061 D tRNA-Thr 466990..467061 Saccharomyces cerevisiae 856586 TV(CAC)H tRNA TV(CAC)H NC_001140.5 475706 475778 R tRNA-Val complement(475706..475778) Saccharomyces cerevisiae 856592 TT(AGU)J tRNA TT(AGU)J NC_001142.7 59100 59172 R tRNA-Thr complement(59100..59172) Saccharomyces cerevisiae 853239 TE(UUC)J tRNA TE(UUC)J NC_001142.7 115935 116006 R tRNA-Glu complement(115935..116006) Saccharomyces cerevisiae 853278 TA(AGC)J tRNA TA(AGC)J NC_001142.7 197311 197383 R tRNA-Ala complement(197311..197383) Saccharomyces cerevisiae 853328 TD(GUC)J1 tRNA TD(GUC)J1 NC_001142.7 204731 204802 D tRNA-Asp 204731..204802 Saccharomyces cerevisiae 853331 TR(ACG)J tRNA TR(ACG)J NC_001142.7 233935 234007 D tRNA-Arg 233935..234007 Saccharomyces cerevisiae 853343 TY(GUA)J1 tRNA SUP7 NC_001142.7 354239 354327 D tRNA-Tyr join(354239..354277,354292..354327) Saccharomyces cerevisiae 853402 TR(UCU)J1 tRNA TR(UCU)J1 NC_001142.7 355369 355440 D tRNA-Arg 355369..355440 Saccharomyces cerevisiae 853403 TD(GUC)J2 tRNA TD(GUC)J2 NC_001142.7 355451 355522 D tRNA-Asp 355451..355522 Saccharomyces cerevisiae 853404 TD(GUC)J3 tRNA TD(GUC)J3 NC_001142.7 374419 374490 R tRNA-Asp complement(374419..374490) Saccharomyces cerevisiae 853411 TR(UCU)J2 tRNA TR(UCU)J2 NC_001142.7 374501 374572 R tRNA-Arg complement(374501..374572) Saccharomyces cerevisiae 853412 TV(AAC)J tRNA TV(AAC)J NC_001142.7 378355 378428 D tRNA-Val 378355..378428 Saccharomyces cerevisiae 853415 TM(CAU)J1 tRNA EMT5 NC_001142.7 391038 391110 R tRNA-Met complement(391038..391110) Saccharomyces cerevisiae 853424 TG(GCC)J1 tRNA TG(GCC)J1 NC_001142.7 396721 396791 R tRNA-Gly complement(396721..396791) Saccharomyces cerevisiae 853430 TK(CUU)J tRNA TK(CUU)J NC_001142.7 414960 415032 D tRNA-Lys 414960..415032 Saccharomyces cerevisiae 853443 TW(CCA)J tRNA TW(CCA)J NC_001142.7 415925 416030 R tRNA-Trp complement(join(415925..415960,415995..416030)) Saccharomyces cerevisiae 853444 TM(CAU)J2 tRNA EMT3 NC_001142.7 422931 423003 D tRNA-Met 422931..423003 Saccharomyces cerevisiae 853448 TL(UAA)J tRNA SUP51 NC_001142.7 424426 424509 R tRNA-Leu complement(424426..424509) Saccharomyces cerevisiae 853451 TM(CAU)J3 tRNA IMT3 NC_001142.7 517806 517877 R tRNA-Met complement(517806..517877) Saccharomyces cerevisiae 853501 TS(AGA)J tRNA TS(AGA)J NC_001142.7 524005 524086 R tRNA-Ser complement(524005..524086) Saccharomyces cerevisiae 853505 TG(GCC)J2 tRNA TG(GCC)J2 NC_001142.7 531821 531891 D tRNA-Gly 531821..531891 Saccharomyces cerevisiae 853511 TR(CCU)J tRNA HSX1 NC_001142.7 538548 538619 D tRNA-Arg 538548..538619 Saccharomyces cerevisiae 853515 TD(GUC)J4 tRNA TD(GUC)J4 NC_001142.7 541501 541572 D tRNA-Asp 541501..541572 Saccharomyces cerevisiae 853517 TY(GUA)J2 tRNA SUP4 NC_001142.7 542949 543037 R tRNA-Tyr complement(join(542949..542984,542999..543037)) Saccharomyces cerevisiae 853519 TL(UAG)J tRNA TL(UAG)J NC_001142.7 617912 618012 D tRNA-Leu join(617912..617949,617969..618012) Saccharomyces cerevisiae 853564 TT(CGU)K tRNA TRT2 NC_001143.7 46736 46807 R tRNA-Thr complement(46736..46807) Saccharomyces cerevisiae 853672 TN(GUU)K tRNA TN(GUU)K NC_001143.7 74629 74702 D tRNA-Asn 74629..74702 Saccharomyces cerevisiae 853637 TL(UAA)K tRNA TL(UAA)K NC_001143.7 84213 84296 D tRNA-Leu 84213..84296 Saccharomyces cerevisiae 853645 TE(UUC)K tRNA TE(UUC)K NC_001143.7 141023 141094 R tRNA-Glu complement(141023..141094) Saccharomyces cerevisiae 853691 TR(UCU)K tRNA TR(UCU)K NC_001143.7 162492 162563 R tRNA-Arg complement(162492..162563) Saccharomyces cerevisiae 853703 TK(CUU)K tRNA TK(CUU)K NC_001143.7 202643 202715 D tRNA-Lys 202643..202715 Saccharomyces cerevisiae 853731 TA(AGC)K1 tRNA TA(AGC)K1 NC_001143.7 219539 219611 D tRNA-Ala 219539..219611 Saccharomyces cerevisiae 853742 TW(CCA)K tRNA TW(CCA)K NC_001143.7 302562 302667 D tRNA-Trp join(302562..302597,302632..302667) Saccharomyces cerevisiae 853791 TV(AAC)K1 tRNA TV(AAC)K1 NC_001143.7 307788 307861 R tRNA-Val complement(307788..307861) Saccharomyces cerevisiae 853795 TH(GUG)K tRNA TH(GUG)K NC_001143.7 313045 313116 R tRNA-His complement(313045..313116) Saccharomyces cerevisiae 853797 TV(AAC)K2 tRNA TV(AAC)K2 NC_001143.7 379323 379396 D tRNA-Val 379323..379396 Saccharomyces cerevisiae 853835 TL(CAA)K tRNA TL(CAA)K NC_001143.7 458200 458313 D tRNA-Leu join(458200..458237,458270..458313) Saccharomyces cerevisiae 853879 TR(ACG)K tRNA TR(ACG)K NC_001143.7 490611 490683 D tRNA-Arg 490611..490683 Saccharomyces cerevisiae 853897 TD(GUC)K tRNA TD(GUC)K NC_001143.7 512974 513045 R tRNA-Asp complement(512974..513045) Saccharomyces cerevisiae 853911 TA(AGC)K2 tRNA TA(AGC)K2 NC_001143.7 517630 517702 D tRNA-Ala 517630..517702 Saccharomyces cerevisiae 853913 TK(UUU)K tRNA TK(UUU)K NC_001143.7 578607 578702 D tRNA-Lys join(578607..578643,578667..578702) Saccharomyces cerevisiae 853948 TP(UGG)L tRNA TP(UGG)L NC_001144.4 92547 92649 D tRNA-Pro join(92547..92582,92614..92649) Saccharomyces cerevisiae 850634 TS(AGA)L tRNA TS(AGA)L NC_001144.4 167943 168024 R tRNA-Ser complement(167943..168024) Saccharomyces cerevisiae 850697 TA(UGC)L tRNA TA(UGC)L NC_001144.4 214884 214956 R tRNA-Ala complement(214884..214956) Saccharomyces cerevisiae 850723 TR(ACG)L tRNA TR(ACG)L NC_001144.4 374356 374428 D tRNA-Arg 374356..374428 Saccharomyces cerevisiae 850804 TD(GUC)L1 tRNA TD(GUC)L1 NC_001144.4 427133 427204 D tRNA-Asp 427133..427204 Saccharomyces cerevisiae 850834 TQ(UUG)L tRNA TQ(UUG)L NC_001144.4 448651 448722 R tRNA-Gln complement(448651..448722) Saccharomyces cerevisiae 850848 TL(UAG)L1 tRNA TL(UAG)L1 NC_001144.4 592521 592621 R tRNA-Leu complement(join(592521..592564,592584..592621)) Saccharomyces cerevisiae 850925 TI(UAU)L tRNA TI(UAU)L NC_001144.4 605302 605434 R tRNA-Ile complement(join(605302..605337,605398..605434)) Saccharomyces cerevisiae 850931 TL(CAA)L tRNA TL(CAA)L NC_001144.4 628385 628499 D tRNA-Leu join(628385..628422,628456..628499) Saccharomyces cerevisiae 850948 TA(AGC)L tRNA TA(AGC)L NC_001144.4 656936 657008 R tRNA-Ala complement(656936..657008) Saccharomyces cerevisiae 850960 TV(AAC)L tRNA TV(AAC)L NC_001144.4 687861 687934 R tRNA-Val complement(687861..687934) Saccharomyces cerevisiae 850978 TL(UAG)L2 tRNA TL(UAG)L2 NC_001144.4 732092 732192 D tRNA-Leu join(732092..732129,732149..732192) Saccharomyces cerevisiae 851009 TI(AAU)L1 tRNA TI(AAU)L1 NC_001144.4 734804 734877 D tRNA-Ile 734804..734877 Saccharomyces cerevisiae 851012 TS(GCU)L tRNA TS(GCU)L NC_001144.4 784354 784453 D tRNA-Ser join(784354..784389,784410..784453) Saccharomyces cerevisiae 851038 TD(GUC)L2 tRNA TD(GUC)L2 NC_001144.4 793918 793989 D tRNA-Asp 793918..793989 Saccharomyces cerevisiae 851044 TE(UUC)L tRNA TE(UUC)L NC_001144.4 797178 797249 D tRNA-Glu 797178..797249 Saccharomyces cerevisiae 851047 TR(CCG)L tRNA TRR4 NC_001144.4 818609 818680 D tRNA-Arg 818609..818680 Saccharomyces cerevisiae 851057 TK(UUU)L tRNA TK(UUU)L NC_001144.4 875375 875470 D tRNA-Lys join(875375..875411,875435..875470) Saccharomyces cerevisiae 851093 TL(UAA)L tRNA TL(UAA)L NC_001144.4 962969 963052 R tRNA-Leu complement(962969..963052) Saccharomyces cerevisiae 851138 TN(GUU)L tRNA TN(GUU)L NC_001144.4 975980 976053 R tRNA-Asn complement(975980..976053) Saccharomyces cerevisiae 851144 TI(AAU)L2 tRNA TI(AAU)L2 NC_001144.4 1052069 1052142 R tRNA-Ile complement(1052069..1052142) Saccharomyces cerevisiae 851176 TR(UCU)M2 tRNA TR(UCU)M2 NC_001145.2 131825 131896 R tRNA-Arg complement(131825..131896) Saccharomyces cerevisiae 854905 TY(GUA)M1 tRNA SUP5 NC_001145.2 168795 168883 D tRNA-Tyr join(168795..168833,168848..168883) Saccharomyces cerevisiae 854951 TG(GCC)M tRNA TG(GCC)M NC_001145.2 183898 183968 R tRNA-Gly complement(183898..183968) Saccharomyces cerevisiae 854961 TP(UGG)M tRNA SUF7 NC_001145.2 196068 196170 R tRNA-Pro complement(join(196068..196103,196135..196170)) Saccharomyces cerevisiae 854967 TS(AGA)M tRNA TS(AGA)M NC_001145.2 259158 259239 R tRNA-Ser complement(259158..259239) Saccharomyces cerevisiae 855007 TE(UUC)M tRNA TE(UUC)M NC_001145.2 290800 290871 D tRNA-Glu 290800..290871 Saccharomyces cerevisiae 855024 TA(AGC)M1 tRNA TA(AGC)M1 NC_001145.2 321146 321218 R tRNA-Ala complement(321146..321218) Saccharomyces cerevisiae 855038 TF(GAA)M tRNA TF(GAA)M NC_001145.2 352279 352369 D tRNA-Phe join(352279..352315,352334..352369) Saccharomyces cerevisiae 855058 TH(GUG)M tRNA TH(GUG)M NC_001145.2 363063 363134 D tRNA-His 363063..363134 Saccharomyces cerevisiae 855065 TV(AAC)M1 tRNA TV(AAC)M1 NC_001145.2 372444 372517 R tRNA-Val complement(372444..372517) Saccharomyces cerevisiae 855069 TW(CCA)M tRNA TW(CCA)M NC_001145.2 379302 379407 R tRNA-Trp complement(join(379302..379337,379372..379407)) Saccharomyces cerevisiae 855072 TV(AAC)M2 tRNA TV(AAC)M2 NC_001145.2 420587 420660 R tRNA-Val complement(420587..420660) Saccharomyces cerevisiae 855100 TD(GUC)M tRNA TD(GUC)M NC_001145.2 463553 463624 D tRNA-Asp 463553..463624 Saccharomyces cerevisiae 855124 TK(CUU)M tRNA TK(CUU)M NC_001145.2 480620 480692 R tRNA-Lys complement(480620..480692) Saccharomyces cerevisiae 855133 TL(CAA)M tRNA TL(CAA)M NC_001145.2 504894 505007 D tRNA-Leu join(504894..504931,504964..505007) Saccharomyces cerevisiae 855146 TM(CAU)M tRNA EMT4 NC_001145.2 572882 572954 R tRNA-Met complement(572882..572954) Saccharomyces cerevisiae 855191 TV(AAC)M3 tRNA TV(AAC)M3 NC_001145.2 586635 586708 R tRNA-Val complement(586635..586708) Saccharomyces cerevisiae 855199 TR(UCU)M1 tRNA TR(UCU)M1 NC_001145.2 747891 747962 R tRNA-Arg complement(747891..747962) Saccharomyces cerevisiae 855279 TA(AGC)M2 tRNA TA(AGC)M2 NC_001145.2 768368 768440 R tRNA-Ala complement(768368..768440) Saccharomyces cerevisiae 855290 TQ(CUG)M tRNA CDC65 NC_001145.2 808245 808316 R tRNA-Gln complement(808245..808316) Saccharomyces cerevisiae 855314 TY(GUA)M2 tRNA SUP8 NC_001145.2 837927 838015 D tRNA-Tyr join(837927..837965,837980..838015) Saccharomyces cerevisiae 855327 TG(UCC)N tRNA SUF6 NC_001146.6 96241 96312 R tRNA-Gly complement(96241..96312) Saccharomyces cerevisiae 855432 TN(GUU)N1 tRNA TN(GUU)N1 NC_001146.6 102716 102789 D tRNA-Asn 102716..102789 Saccharomyces cerevisiae 855435 TT(AGU)N1 tRNA TT(AGU)N1 NC_001146.6 104805 104877 D tRNA-Thr 104805..104877 Saccharomyces cerevisiae 855437 TF(GAA)N tRNA TF(GAA)N NC_001146.6 374871 374961 D tRNA-Phe join(374871..374907,374926..374961) Saccharomyces cerevisiae 855590 TL(CAA)N tRNA TL(CAA)N NC_001146.6 443008 443121 D tRNA-Leu join(443008..443045,443078..443121) Saccharomyces cerevisiae 855627 TD(GUC)N tRNA TD(GUC)N NC_001146.6 519100 519170 R tRNA-Asp complement(519100..519170) Saccharomyces cerevisiae 855670 TP(UGG)N1 tRNA TP(UGG)N1 NC_001146.6 547095 547197 D tRNA-Pro join(547095..547130,547162..547197) Saccharomyces cerevisiae 855685 TT(AGU)N2 tRNA TT(AGU)N2 NC_001146.6 560694 560766 R tRNA-Thr complement(560694..560766) Saccharomyces cerevisiae 855693 TP(UGG)N2 tRNA TP(UGG)N2 NC_001146.6 568116 568218 D tRNA-Pro join(568116..568151,568183..568218) Saccharomyces cerevisiae 855694 TI(AAU)N1 tRNA TI(AAU)N1 NC_001146.6 569868 569941 R tRNA-Ile complement(569868..569941) Saccharomyces cerevisiae 855696 TI(AAU)N2 tRNA TI(AAU)N2 NC_001146.6 602313 602386 D tRNA-Ile 602313..602386 Saccharomyces cerevisiae 855714 TP(AGG)N tRNA SUF10 NC_001146.6 631848 631919 R tRNA-Pro complement(631848..631919) Saccharomyces cerevisiae 855734 TN(GUU)N2 tRNA TN(GUU)N2 NC_001146.6 632601 632674 D tRNA-Asn 632601..632674 Saccharomyces cerevisiae 855735 TL(UAA)N tRNA TL(UAA)N NC_001146.6 726135 726218 R tRNA-Leu complement(726135..726218) Saccharomyces cerevisiae 855791 TG(UCC)O tRNA SUF1 NC_001147.5 110962 111033 R tRNA-Gly complement(110962..111033) Saccharomyces cerevisiae 854041 TT(AGU)O1 tRNA TT(AGU)O1 NC_001147.5 113802 113874 D tRNA-Thr 113802..113874 Saccharomyces cerevisiae 854045 TG(GCC)O1 tRNA TG(GCC)O1 NC_001147.5 226610 226680 R tRNA-Gly complement(226610..226680) Saccharomyces cerevisiae 854100 TN(GUU)O1 tRNA TN(GUU)O1 NC_001147.5 228330 228403 D tRNA-Asn 228330..228403 Saccharomyces cerevisiae 854101 TS(GCU)O tRNA TS(GCU)O NC_001147.5 274674 274774 D tRNA-Ser join(274674..274710,274730..274774) Saccharomyces cerevisiae 854132 TG(GCC)O2 tRNA SUF17 NC_001147.5 282165 282235 D tRNA-Gly 282165..282235 Saccharomyces cerevisiae 854137 TY(GUA)O tRNA SUP3 NC_001147.5 288193 288281 D tRNA-Tyr join(288193..288231,288246..288281) Saccharomyces cerevisiae 854140 TP(UGG)O1 tRNA TP(UGG)O1 NC_001147.5 301098 301199 D tRNA-Pro join(301098..301133,301164..301199) Saccharomyces cerevisiae 854148 TR(ACG)O tRNA TR(ACG)O NC_001147.5 340300 340372 R tRNA-Arg complement(340300..340372) Saccharomyces cerevisiae 854169 TT(AGU)O2 tRNA TT(AGU)O2 NC_001147.5 354041 354113 R tRNA-Thr complement(354041..354113) Saccharomyces cerevisiae 854176 TK(UUU)O tRNA TK(UUU)O NC_001147.5 438645 438740 D tRNA-Lys join(438645..438681,438705..438740) Saccharomyces cerevisiae 854224 TP(UGG)O2 tRNA SUF11 NC_001147.5 464452 464553 D tRNA-Pro join(464452..464487,464518..464553) Saccharomyces cerevisiae 854239 TN(GUU)O2 tRNA TN(GUU)O2 NC_001147.5 487441 487514 D tRNA-Asn 487441..487514 Saccharomyces cerevisiae 854254 TD(GUC)O tRNA TD(GUC)O NC_001147.5 571959 572030 D tRNA-Asp 571959..572030 Saccharomyces cerevisiae 854298 TG(CCC)O tRNA SUF5 NC_001147.5 594355 594426 R tRNA-Gly complement(594355..594426) Saccharomyces cerevisiae 854311 TV(AAC)O tRNA TV(AAC)O NC_001147.5 663813 663886 R tRNA-Val complement(663813..663886) Saccharomyces cerevisiae 854348 TM(CAU)O1 tRNA IMT1 NC_001147.5 710202 710273 D tRNA-Met 710202..710273 Saccharomyces cerevisiae 854367 TA(UGC)O tRNA TA(UGC)O NC_001147.5 854186 854258 R tRNA-Ala complement(854186..854258) Saccharomyces cerevisiae 854464 TM(CAU)O2 tRNA EMT2 NC_001147.5 976418 976490 D tRNA-Met 976418..976490 Saccharomyces cerevisiae 854524 TP(UGG)O3 tRNA TP(UGG)O3 NC_001147.5 980680 980784 D tRNA-Pro join(980680..980715,980749..980784) Saccharomyces cerevisiae 854526 TW(CCA)P tRNA TW(CCA)P NC_001148.3 56169 56274 R tRNA-Trp complement(join(56169..56204,56239..56274)) Saccharomyces cerevisiae 855815 TE(UUC)P tRNA TE(UUC)P NC_001148.3 210191 210262 R tRNA-Glu complement(210191..210262) Saccharomyces cerevisiae 855924 TM(CAU)P tRNA IMT2 NC_001148.3 338847 338918 R tRNA-Met complement(338847..338918) Saccharomyces cerevisiae 855992 TC(GCA)P1 tRNA TC(GCA)P1 NC_001148.3 435890 435961 R tRNA-Cys complement(435890..435961) Saccharomyces cerevisiae 856046 TF(GAA)P1 tRNA TF(GAA)P1 NC_001148.3 560195 560286 R tRNA-Phe complement(join(560195..560230,560250..560286)) Saccharomyces cerevisiae 856109 TG(GCC)P1 tRNA TG(GCC)P1 NC_001148.3 572266 572336 D tRNA-Gly 572266..572336 Saccharomyces cerevisiae 856116 TK(CUU)P tRNA TK(CUU)P NC_001148.3 582059 582131 D tRNA-Lys 582059..582131 Saccharomyces cerevisiae 856120 TF(GAA)P2 tRNA TF(GAA)P2 NC_001148.3 622537 622628 R tRNA-Phe complement(join(622537..622572,622592..622628)) Saccharomyces cerevisiae 856139 TS(UGA)P tRNA SUP16 NC_001148.3 689562 689643 D tRNA-Ser 689562..689643 Saccharomyces cerevisiae 856185 TT(UGU)P tRNA TT(UGU)P NC_001148.3 744282 744353 D tRNA-Thr 744282..744353 Saccharomyces cerevisiae 856224 TK(UUU)P tRNA TK(UUU)P NC_001148.3 769205 769300 R tRNA-Lys complement(join(769205..769240,769264..769300)) Saccharomyces cerevisiae 856235 TC(GCA)P2 tRNA TC(GCA)P2 NC_001148.3 775763 775834 R tRNA-Cys complement(775763..775834) Saccharomyces cerevisiae 856238 TN(GUU)P tRNA TN(GUU)P NC_001148.3 810672 810745 D tRNA-Asn 810672..810745 Saccharomyces cerevisiae 856259 TI(AAU)P1 tRNA TI(AAU)P1 NC_001148.3 819525 819598 D tRNA-Ile 819525..819598 Saccharomyces cerevisiae 856266 TA(AGC)P tRNA TA(AGC)P NC_001148.3 856898 856970 D tRNA-Ala 856898..856970 Saccharomyces cerevisiae 856286 TG(GCC)P2 tRNA TG(GCC)P2 NC_001148.3 860375 860445 D tRNA-Gly 860375..860445 Saccharomyces cerevisiae 856288 TI(AAU)P2 tRNA TI(AAU)P2 NC_001148.3 880292 880365 R tRNA-Ile complement(880292..880365) Saccharomyces cerevisiae 856299 4932000001 rRNA 4932000001 NC_001224.1 6546 8194 D 15S_RRNA 6546..8194 Saccharomyces cerevisiae 4932000002 rRNA 4932000002 NC_001224.1 58009 62447 D 21S_RRNA 58009..62447 Saccharomyces cerevisiae 4932000003 rRNA 4932000003 NC_001144.4 451576 458433 R RDN37-1 complement(451576..458433) Saccharomyces cerevisiae 4932000004 rRNA 4932000004 NC_001144.4 451576 451786 R ETS2-1 complement(451576..451786) Saccharomyces cerevisiae 4932000005 rRNA 4932000005 NC_001144.4 451787 455182 R RDN25-1 complement(451787..455182) Saccharomyces cerevisiae 4932000006 rRNA 4932000006 NC_001144.4 455183 455414 R ITS2-1 complement(455183..455414) Saccharomyces cerevisiae 4932000007 rRNA 4932000007 NC_001144.4 455415 455572 R RDN58-1 complement(455415..455572) Saccharomyces cerevisiae 4932000008 rRNA 4932000008 NC_001144.4 455573 455933 R ITS1-1 complement(455573..455933) Saccharomyces cerevisiae 4932000009 rRNA 4932000009 NC_001144.4 455934 457733 R RDN18-1 complement(455934..457733) Saccharomyces cerevisiae 4932000010 rRNA 4932000010 NC_001144.4 457734 458433 R ETS1-1 complement(457734..458433) Saccharomyces cerevisiae 4932000011 rRNA 4932000011 NC_001144.4 458434 459676 R NTS2-1 complement(458434..459676) Saccharomyces cerevisiae 4932000012 rRNA 4932000012 NC_001144.4 459677 459797 D RDN5-1 459677..459797 Saccharomyces cerevisiae 4932000013 rRNA 4932000013 NC_001144.4 459798 460712 R NTS1-2 complement(459798..460712) Saccharomyces cerevisiae 4932000014 rRNA 4932000014 NC_001144.4 460713 467570 R RDN37-2 complement(460713..467570) Saccharomyces cerevisiae 4932000015 rRNA 4932000015 NC_001144.4 460713 460923 R ETS2-2 complement(460713..460923) Saccharomyces cerevisiae 4932000016 rRNA 4932000016 NC_001144.4 460924 464319 R RDN25-2 complement(460924..464319) Saccharomyces cerevisiae 4932000017 rRNA 4932000017 NC_001144.4 464320 464551 R ITS2-2 complement(464320..464551) Saccharomyces cerevisiae 4932000018 rRNA 4932000018 NC_001144.4 464552 464709 R RDN58-2 complement(464552..464709) Saccharomyces cerevisiae 4932000019 rRNA 4932000019 NC_001144.4 464710 465070 R ITS1-2 complement(464710..465070) Saccharomyces cerevisiae 4932000020 rRNA 4932000020 NC_001144.4 465071 466870 R RDN18-2 complement(465071..466870) Saccharomyces cerevisiae 4932000021 rRNA 4932000021 NC_001144.4 466871 467570 R ETS1-2 complement(466871..467570) Saccharomyces cerevisiae 4932000022 rRNA 4932000022 NC_001144.4 467571 468813 R NTS2-2 complement(467571..468813) Saccharomyces cerevisiae 4932000023 rRNA 4932000023 NC_001144.4 468814 468932 D RDN5-2 468814..468932 Saccharomyces cerevisiae 4932000024 rRNA 4932000024 NC_001144.4 472466 472584 D RDN5-3 472466..472584 Saccharomyces cerevisiae 4932000025 rRNA 4932000025 NC_001144.4 482046 482164 D RDN5-4 482046..482164 Saccharomyces cerevisiae 4932000026 rRNA 4932000026 NC_001144.4 485698 485816 D RDN5-5 485698..485816 Saccharomyces cerevisiae 4932000027 rRNA 4932000027 NC_001144.4 489350 489470 D RDN5-6 489350..489470 Saccharomyces cerevisiae